LOCUS       DAWHXZ010000001        38711 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000001 DAWHXZ010000000
VERSION     DAWHXZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38711
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.001"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            294..938
                     /gene="rplC"
                     /locus_tag="WIE35_000001"
     CDS             294..938
                     /gene="rplC"
                     /locus_tag="WIE35_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ4152996.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            938..1558
                     /gene="rplD"
                     /locus_tag="WIE35_000002"
     CDS             938..1558
                     /gene="rplD"
                     /locus_tag="WIE35_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ4152997.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1555..1869
                     /gene="rplW"
                     /locus_tag="WIE35_000003"
     CDS             1555..1869
                     /gene="rplW"
                     /locus_tag="WIE35_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ4152998.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            1875..2708
                     /gene="rplB"
                     /locus_tag="WIE35_000004"
     CDS             1875..2708
                     /gene="rplB"
                     /locus_tag="WIE35_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ4152999.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     SNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQVGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2715..2993
                     /gene="rpsS"
                     /locus_tag="WIE35_000005"
     CDS             2715..2993
                     /gene="rpsS"
                     /locus_tag="WIE35_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ4153000.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3002..3331
                     /gene="rplV"
                     /locus_tag="WIE35_000006"
     CDS             3002..3331
                     /gene="rplV"
                     /locus_tag="WIE35_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ4153001.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3341..4033
                     /gene="rpsC"
                     /locus_tag="WIE35_000007"
     CDS             3341..4033
                     /gene="rpsC"
                     /locus_tag="WIE35_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ4153002.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4017..4433
                     /gene="rplP"
                     /locus_tag="WIE35_000008"
     CDS             4017..4433
                     /gene="rplP"
                     /locus_tag="WIE35_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ4153003.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4433..4624
                     /gene="rpmC"
                     /locus_tag="WIE35_000009"
     CDS             4433..4624
                     /gene="rpmC"
                     /locus_tag="WIE35_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ4153004.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4624..4887
                     /gene="rpsQ"
                     /locus_tag="WIE35_000010"
     CDS             4624..4887
                     /gene="rpsQ"
                     /locus_tag="WIE35_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ4153005.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5109..5477
                     /gene="rplN"
                     /locus_tag="WIE35_000011"
     CDS             5109..5477
                     /gene="rplN"
                     /locus_tag="WIE35_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ4153006.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5489..5812
                     /gene="rplX"
                     /locus_tag="WIE35_000012"
     CDS             5489..5812
                     /gene="rplX"
                     /locus_tag="WIE35_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ4153007.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5822..6361
                     /gene="rplE"
                     /locus_tag="WIE35_000013"
     CDS             5822..6361
                     /gene="rplE"
                     /locus_tag="WIE35_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ4153008.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6364..6669
                     /gene="rpsN"
                     /locus_tag="WIE35_000014"
     CDS             6364..6669
                     /gene="rpsN"
                     /locus_tag="WIE35_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ4153009.1"
                     /translation="MAKKALINRNLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6685..7077
                     /gene="rpsH"
                     /locus_tag="WIE35_000015"
     CDS             6685..7077
                     /gene="rpsH"
                     /locus_tag="WIE35_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ4153010.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYADRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7091..7624
                     /gene="rplF"
                     /locus_tag="WIE35_000016"
     CDS             7091..7624
                     /gene="rplF"
                     /locus_tag="WIE35_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ4153011.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7638..7991
                     /gene="rplR"
                     /locus_tag="WIE35_000017"
     CDS             7638..7991
                     /gene="rplR"
                     /locus_tag="WIE35_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ4153012.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8010..8528
                     /gene="rpsE"
                     /locus_tag="WIE35_000018"
     CDS             8010..8528
                     /gene="rpsE"
                     /locus_tag="WIE35_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ4153013.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8521..8706
                     /gene="rpmD"
                     /locus_tag="WIE35_000019"
     CDS             8521..8706
                     /gene="rpmD"
                     /locus_tag="WIE35_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ4153014.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8708..9142
                     /gene="rplO"
                     /locus_tag="WIE35_000020"
     CDS             8708..9142
                     /gene="rplO"
                     /locus_tag="WIE35_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ4153015.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9154..10464
                     /gene="secY"
                     /locus_tag="WIE35_000021"
     CDS             9154..10464
                     /gene="secY"
                     /locus_tag="WIE35_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ4153016.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10469..10687
                     /gene="infA"
                     /locus_tag="WIE35_000022"
     CDS             10469..10687
                     /gene="infA"
                     /locus_tag="WIE35_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ4153017.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10708..10821
                     /gene="rpmJ"
                     /locus_tag="WIE35_000023"
     CDS             10708..10821
                     /gene="rpmJ"
                     /locus_tag="WIE35_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ4153018.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            10887..11249
                     /gene="rpsM"
                     /locus_tag="WIE35_000024"
     CDS             10887..11249
                     /gene="rpsM"
                     /locus_tag="WIE35_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ4153019.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11269..11664
                     /gene="rpsK"
                     /locus_tag="WIE35_000025"
     CDS             11269..11664
                     /gene="rpsK"
                     /locus_tag="WIE35_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ4153020.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11684..12304
                     /gene="rpsD"
                     /locus_tag="WIE35_000026"
     CDS             11684..12304
                     /gene="rpsD"
                     /locus_tag="WIE35_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ4153021.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12330..13316
                     /gene="rpoA"
                     /locus_tag="WIE35_000027"
     CDS             12330..13316
                     /gene="rpoA"
                     /locus_tag="WIE35_000027"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ4153022.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13340..13708
                     /gene="rplQ"
                     /locus_tag="WIE35_000028"
     CDS             13340..13708
                     /gene="rplQ"
                     /locus_tag="WIE35_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ4153023.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            13855..14568
                     /gene="minC"
                     /locus_tag="WIE35_000029"
     CDS             13855..14568
                     /gene="minC"
                     /locus_tag="WIE35_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ4153024.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSDERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14597..15412
                     /gene="minD"
                     /locus_tag="WIE35_000030"
     CDS             14597..15412
                     /gene="minD"
                     /locus_tag="WIE35_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ4153025.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15416..15679
                     /gene="minE"
                     /locus_tag="WIE35_000031"
     CDS             15416..15679
                     /gene="minE"
                     /locus_tag="WIE35_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ4153026.1"
                     /translation="MSLIELLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVDVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15683..16603
                     /gene="oxyR"
                     /locus_tag="WIE35_000032"
     CDS             15683..16603
                     /gene="oxyR"
                     /locus_tag="WIE35_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ4153027.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(16600..16791)
                     /locus_tag="WIE35_000033"
     CDS             complement(16600..16791)
                     /locus_tag="WIE35_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153028.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(16933..19770)
                     /locus_tag="WIE35_000034"
     CDS             complement(16933..19770)
                     /locus_tag="WIE35_000034"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ4153029.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHTFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
     gene            complement(19867..20676)
                     /gene="zupT"
                     /locus_tag="WIE35_000035"
     CDS             complement(19867..20676)
                     /gene="zupT"
                     /locus_tag="WIE35_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ4153030.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTTGMAVIAVSLVLFHF"
     gene            complement(20857..22113)
                     /locus_tag="WIE35_000036"
     CDS             complement(20857..22113)
                     /locus_tag="WIE35_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ4153031.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCHLFTENLYKLCQEKGVQF
                     HFNQTISRIDHNGLRIKAVETKTGRFEADAVVCALGCFSRTVLAQLDLDLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTHFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(22192..23583)
                     /locus_tag="WIE35_000037"
     CDS             complement(22192..23583)
                     /locus_tag="WIE35_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ4153032.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVLLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWITAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(23940..24716)
                     /gene="lpxA"
                     /locus_tag="WIE35_000038"
     CDS             complement(23940..24716)
                     /gene="lpxA"
                     /locus_tag="WIE35_000038"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ4153033.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKNVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSTRGIIR"
     gene            complement(24759..25439)
                     /locus_tag="WIE35_000039"
     CDS             complement(24759..25439)
                     /locus_tag="WIE35_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEZ4153034.1"
                     /translation="MERYKNAVGEDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDRNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDVTGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINKFRNRLAHHEPICFKGAIKDTNYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(25646..26095)
                     /gene="fabZ"
                     /locus_tag="WIE35_000040"
     CDS             complement(25646..26095)
                     /gene="fabZ"
                     /locus_tag="WIE35_000040"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ4153035.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            complement(26129..27175)
                     /gene="lpxD"
                     /locus_tag="WIE35_000041"
     CDS             complement(26129..27175)
                     /gene="lpxD"
                     /locus_tag="WIE35_000041"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ4153036.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCRLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(27208..27708)
                     /locus_tag="WIE35_000042"
     CDS             complement(27208..27708)
                     /locus_tag="WIE35_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ4153037.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKVQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(27774..30167)
                     /gene="bamA"
                     /locus_tag="WIE35_000043"
     CDS             complement(27774..30167)
                     /gene="bamA"
                     /locus_tag="WIE35_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ4153038.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKQYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEIGYANGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(30224..31564)
                     /gene="rseP"
                     /locus_tag="WIE35_000044"
     CDS             complement(30224..31564)
                     /gene="rseP"
                     /locus_tag="WIE35_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ4153039.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(31599..32834)
                     /locus_tag="WIE35_000045"
     CDS             complement(31599..32834)
                     /locus_tag="WIE35_000045"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ4153040.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPA
                     QAVKHPNWSMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(32839..33636)
                     /locus_tag="WIE35_000046"
     CDS             complement(32839..33636)
                     /locus_tag="WIE35_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ4153041.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLSGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(33639..34385)
                     /locus_tag="WIE35_000047"
     CDS             complement(33639..34385)
                     /locus_tag="WIE35_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ4153042.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(34441..34998)
                     /gene="frr"
                     /locus_tag="WIE35_000048"
     CDS             complement(34441..34998)
                     /gene="frr"
                     /locus_tag="WIE35_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ4153043.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(35178..36305)
                     /locus_tag="WIE35_000049"
     CDS             complement(35178..36305)
                     /locus_tag="WIE35_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ4153044.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(36379..36454)
                     /locus_tag="WIE35_000050"
     tRNA            complement(36379..36454)
                     /locus_tag="WIE35_000050"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36419..36421),aa:Thr,seq:cgt)
     gene            36561..36755
                     /locus_tag="WIE35_000051"
     CDS             36561..36755
                     /locus_tag="WIE35_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HEZ4153045.1"
                     /translation="MFVNEKDPYAALFAGLVFLTLPFALAVHDAFALAFGRAGLLVSV
                     SAGVAVGTVLFGLCSVCLRF"
     gene            36752..37033
                     /locus_tag="WIE35_000052"
     CDS             36752..37033
                     /locus_tag="WIE35_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153046.1"
                     /translation="MNLVVSAGLTKLAYKKMMRRHSRYALFLSGVAACAAAAVAWIFE
                     LLLGSGALGGLRGEAVLEYAFAVWLVAMLTLPKRLTRAPVQPVVFHRKK"
     gene            37129..37752
                     /gene="rsmG"
                     /locus_tag="WIE35_000053"
     CDS             37129..37752
                     /gene="rsmG"
                     /locus_tag="WIE35_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ4153047.1"
                     /translation="MERKERLQSGIAAMGLDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQGEIDHLPQDVCVEKVQRLDVPGLDAERHIVILRKR"
     gene            37851..38624
                     /locus_tag="WIE35_000054"
     CDS             37851..38624
                     /locus_tag="WIE35_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ4153048.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELAARVSGK"
BASE COUNT         9087 a         8744 c        10656 g        10224 t
ORIGIN      
        1 tttgacgaac tgagcatccc cgattacagt catcaaccat attccggttt gttggagaaa
       61 gatgcatacg ctgtgatgac cggataccga ccctttaaaa gagtctgacc ctatgccgtc
      121 tgaaaattca aaacgcttca gacggcatat tgaagatatt tctgatattt ctgttgatat
      181 ttctttaact tgtcagatat aatgccgagc ttggtacatt tgtgccaagt ttaactttgt
      241 ctgaaagaca ggccaatcgt agcctgtccc tttactttaa aaggaaaata atcatgactt
      301 taggtctggt tggacgcaaa gttggtatga cccgcgtgtt cgacgaacag ggtgtttctg
      361 ttccggtaac cgttttggat atgtctgcca accgcgttac acaagtaaaa tccaaagata
      421 ctgacggcta tactgccgtt caagttacct ttggtcagaa aaaagccaat cgtgtcaaca
      481 aagccgaagc cgggcacttt gcaaaagcag gtgttgaagc cggtcgcggt ttgattgagt
      541 ttgctttgac tgaagaaaaa ctggctgaat tgaaagctgg tgacgaaatc accgtttcta
      601 tgtttgaagt cggtcaactg gtcgatgtaa ccggtacctc taaaggtaaa ggtttctccg
      661 gcacgattaa acgtcataac ttcggcgccc aacgtacttc ccacggtaac tcccgctctc
      721 accgcgttcc aggttctatc ggtatggcac aagacccggg tcgcgtgttc cccggtaagc
      781 gcatggccgg ccaatacggc aacaccaagg caactgttca aaaattggaa gttgtccgtg
      841 ttgatgcaga acgccaactg ctgttggtta agggtgctgt tccgggtgcg gtcaacagcg
      901 atgttgtagt tcgtcccagc gtgaaagtag gtgcgtaatg gaattgaaag taattgacgc
      961 taaaggacaa gtttcaggca gcctgtctgt ttctgatgct ttgttcgccc gcgaatacaa
     1021 tgaagcgttg gttcatcagc tggtaaatgc ctacttggca aacgcccgct ccggtaaccg
     1081 cgctcaaaaa acccgtgccg aagtaaaaca ctcaaccaaa aaaccatggc gtcaaaaagg
     1141 taccggccgt gcccgttccg gtatgacttc ttctccgctg tggcgtaaag gtggtcgcgc
     1201 gttcccgaac aaacccgacg aaaacttcac tcaaaaagta aaccgcaaaa tgtaccgtgc
     1261 cggtatggcg actattctgt cccaattgac tcgtgacgag cgtttgtttg cgattgaggc
     1321 gttgactgcc gaaactccta aaaccaaagt ttttgctgaa caagtgaaaa atctgggtct
     1381 agagcaagtg ttgtttgtaa ccaaacagct cgacgagaat gtttacttgg cttcacgcaa
     1441 cttgccaaac gtgttggttt tggaagctca acaagttgat ccttacagct tgctgcgtta
     1501 caaaaaagta atcatcacta aagatgcagt tgcacaatta gaggagcaat gggtatgaat
     1561 caacaacgtt tgactcaagt gattttggca cctatcgttt ctgaaaaaag caacgtattg
     1621 gctgaaaaac gcaaccaaat gacgtttaaa gttttggcaa atgcaaccaa acctgaaatc
     1681 aaagcggctg ttgagctgct gtttggtgtt caagttgctt ctgtaactac cgttacaact
     1741 aaaggcaaaa ctaagcgttt tggtcgtact ttgggccgcc gcagcgatgt taaaaaagct
     1801 tatgtaagct tggctgccgg tcaagagttg gatttggaag ccgctgctgc agctgcagat
     1861 aaggaataaa caaaatggca atcgttaaaa tgaagccgac ctctgcaggc cgtcgcggca
     1921 tggttcgcgt ggtaacagaa ggtttgcaca aaggtgcgcc ttatgcacct ttgctcgaaa
     1981 agaaaaattc tactgccggt cgtaacagta atggtcatat caccacccgt cacaaaggcg
     2041 gcggtcataa acaccattac cgtgttgtag actttaaacg taacaaagac ggcattcctg
     2101 ctaaggtaga gcgtatcgaa tacgatccta accgtactgc cttcatcgca ctgttgtgct
     2161 atgcagacgg cgagcgtcgt tacatcatcg ctcctcgcgg tattcaagtc ggtgccgtat
     2221 tggtttccgg tgctgaagct gccatcaaag taggcaacac cctgccgatc cgcaacattc
     2281 ccgttggtac gactatccac tgtatcgaaa tgaaacccgg taaaggtgct caaatcgcac
     2341 gttctgccgg tgcttctgcg gtattgctgg ctaaagaagg cgcgtacgct caagtccgtc
     2401 tgcgctctgg cgaagttcgt aaaatcaacg taaattgccg tgcgaccatc ggtgaagttg
     2461 gtaacgaaga gcaaagcctg aaaaaaatcg gtaaagccgg tgccaaccgt tggcgcggta
     2521 ttcgtccgac cgtacgcggt gttgtcatga atcctgtcga tcacccgcat ggtggtggtg
     2581 aaggccgtac gggcgaggcc cgcgaaccgg tcagcccatg gggtactcct gctaaaggct
     2641 accgcactcg taataacaaa cgcacggata acatgattgt tcgtcgccgt tactcaaata
     2701 aaggttaatt tagtatggct cgttcattga aaaaaggccc atatgtagac ctgcatttgc
     2761 tgaaaaaagt agatgctgct cgcgcaagca acgacaaacg cccgattaaa acctggtctc
     2821 gtcgttctac cattctgcct gattttatcg gtctgaccat tgctgtgcac aacggccgca
     2881 cccatgtgcc tgtgtttatc agcgacaata tggttggtca taaattaggc gaattctcat
     2941 tgacccgtac ctttaaaggc cacttggccg ataaaaaggc taaaaagaaa taaggtgaat
     3001 catgagagta aatgcacaac ataaaaatgc ccgtatctct gctcaaaagg ctcgtttggt
     3061 agctgatttg attcgtggta aagacgttgc ccaagctttg aatattttgg ctttcagtcc
     3121 taaaaaaggt gccgagctga ttaaaaaagt attggagtca gctattgcta atgccgagca
     3181 caataacggt gcggacattg atgaactgaa agtggtaact atctttgttg acaaaggccc
     3241 aagcttgaaa cgttttcaag ctcgcgccaa aggtcgcggt aaccgcatcg aaaaacaaac
     3301 ttgtcatatc aatgtgacag tgggtaacta aggaaaagct atgggacaaa agattaaccc
     3361 tacaggcttt cgcctggcgg taactaaaga ctgggcttca aaatggtttg ctaaaagcac
     3421 cgacttttct actgttttga agcaggatat cgatgttcgc aattatttgc gtcaaaaatt
     3481 ggccaatgct tcggttggtc gagtggttat tgaacgccct gcaaaatctg cacgcattac
     3541 cattcactcc gctcgtccgg gtgtggttat cggtaaaaaa ggtgaggata tcgaggtttt
     3601 gaaacgtgac ttgcaagtct tgatgggtgt acctgttcat gtaaatattg aagagattcg
     3661 ccgtcctgag ttggatgctc aaattattgc tgacggtatt gcccagcagt tggaaaagcg
     3721 cgttcaattc cgtcgtgcta tgaaacgagc aatgcaaaat gcaatgcgtt ctggtgctaa
     3781 aggcattaag attatgactt caggccgtct gaatggtgcg gatattgccc gtagcgaatg
     3841 gtatcgtgaa ggtcgtgtgc cactgcatac tttacgtgca aatgtagatt atgcaaccag
     3901 cgaagcgcac accacatatg gtgtattggg tctgaaagtt tgggtttata cggaaggcaa
     3961 tattaaatct tccaaacctg aacatgagag taaacaaaga aaggcaggta gacgtaatgc
     4021 tgcagccaac tagactgaaa taccgtaagc aacaaaaggg tcgcaatact ggcatcgcta
     4081 ctcgcggtaa taaggtaagt ttcggtgagt tcggcttgaa agccgtaggt cgtggtcgtt
     4141 tgactgcccg tcaaatcgaa gctgctcgtc gtgcaatgac ccgtcatatc aaacgtggtg
     4201 gtcgtatttg gattcgtgta ttccctgata aaccgattac tgaaaagcct attcaagttc
     4261 gtatgggtgg cggtaaaggt aacgtggaat attacattgc cgaaattaaa ccaggtaaag
     4321 tgttgtatga aatggatggc gttccagagg aactggctcg tgaagcattc gagttggctg
     4381 ctgccaaatt gcctattcct acaacctttg tagtaagaca ggtgggtcaa taatgaaagc
     4441 aaatgaattg aaagacaaat ccgttgagca gttgaatgca gatttgttgg acttgttgaa
     4501 agctcagttt ggcttacgta tgcaaaacgc taccggtcaa ttaggcaaac caagtgaatt
     4561 gaaacgtgta cgtcgcgata ttgctcgtat taaaaccgtt ttaactgaaa aaggtgctaa
     4621 gtaatgagcg aaactaaaaa tgttcgtact ttgcaaggca aagtagtaag cgacaaaatg
     4681 gataaaaccg taacagtatt ggttgagcgt aaagtaaaac atccgctgta tggtaagatt
     4741 attcggttat ctactaaaat ccatgcccat gatgaaaata atcaatatgg aattggtgat
     4801 gtggttgtta tatcggaatc ccgtccattg tcaaaaacta aatcttgggt tgtcagtgag
     4861 ctggttgaga aagcacgttc tatttaagaa ttaaagcaac gtgcttggaa tgggaaacga
     4921 agtattgcag caaatttaat ttgcgtgtaa acttcgtttc ctgtctttca gtttcttctg
     4981 gaagtttctt ccctttcggg gtccaagact ggtttacttg aaccgcaagg tttcatttaa
     5041 taagcagcgg ctttgctgta agttatctga aagtggtaaa ttaagttggt taatttaaag
     5101 gtaataacat gattcaaatg cagaccatct tagatgtggc tgataactct ggtgcgcgtc
     5161 gcgtaatgtg tatcaaggta ttgggcggat ctaagcgtcg ctacgcttct gttggcgata
     5221 ttattaaagt ggcagttaaa gatgcggctc cgcgtggccg tgtcaaaaaa ggcgatgtat
     5281 ataatgcggt agttgttcgt actgctaagg gtgtacgtcg tcctgatggt gcgttaatta
     5341 aattcgataa caatgccgcc gtgttactga ataataaact tgaacctttg ggtactcgta
     5401 tctttggtcc ggtaacccgt gaattgcgta ctgagcgatt tatgaaaatc gtttcattgg
     5461 cacctgaagt attataagga atggcacgat gaataaaatc attaaaggcg atagggttgt
     5521 agtaattgct ggtaaggata aaggtaagca gggtcaagta gttcgagtgt tgggtgataa
     5581 agttgttgtt gagggcgtta atgttgtaaa acgccatcaa aaacctaatc caatgcgtgg
     5641 cattgagggc ggtattatta ctaaagaaat gcctttggat atttctaata tcgcaatcct
     5701 gaatccggaa actaataaag cggaccgtgt tggtattaag ctgattgaaa atgaaggcaa
     5761 agttaaacgc gttcgtttct tcaaatcaaa tggctctatc attggggcat aaggagataa
     5821 catggctcgg ttgagagagt tttataaaga gacagttgtt cctgaattgg ttaaacaatt
     5881 tggttacaaa tcagtaatgg aagtcccgcg tattgaaaaa atcaccttga atatgggtgt
     5941 gggtgaggct gttgctgaca aaaaagttat ggaacatgct gtttccgatt tagagaaaat
     6001 tgccggtcaa aaaccggttg ttactgttgc ccgtaaatct atcgcaggtt ttaaaatccg
     6061 tgataactat ccggttggtt gcaaagtaac attgcgtcgt gatcaaatgt ttgaattctt
     6121 ggatcgtttg attactattg cattacctcg cgtacgtgac ttccgtggtg tgagcggtaa
     6181 atcatttgat ggccgtggca attacaatat gggtgttcgt gagcaaatta tttttccgga
     6241 aattgaatac gataaaattg atgctttgcg tggtttgaat attactatta ctactacagc
     6301 aaaaaccgat gaggaagcga aagctttatt gtcattgttt aaatttccgt tcaaaggata
     6361 atcatggcta agaaagcact tattaatcgt aatctgaaac gtcaagcttt ggctaaaaaa
     6421 tatgcggcta aacgcgcggc aattaaagcg gtaatcaatg attcgaatgc aactgaggaa
     6481 gagcgttttg aggctcgttt gaggtttcaa tccattcctc gtaatgcggc acctgtgcgt
     6541 caacgtcgtc gttgtgcttt gacaggtcgc cctcgtggta ctttccgtaa atttggtttg
     6601 ggtcgtatta aaatccgtga aatcgccatg cgtggcgaaa ttccgggtgt tgttaaagcc
     6661 agctggtaat aggagtaatt aagaatgagt atgcatgatc ctatttccga tatgttgact
     6721 cgtatccgca atgcgcaacg tgctaataaa gcagcggttg caatgccttc ttcaaaatta
     6781 aagtgtgcta ttgcaaaggt attgaaagaa gaaggatata ttgaggactt cgcagtttca
     6841 tctgacgtaa agtctatatt ggaaattcaa ttaaaatact atgcagatcg tcctgtaatt
     6901 gaacaaatca agcgtgtatc tcgccccggt ttgcgtattt ataaagcgtc tagtgagatt
     6961 ccaagtgtta tgaatggctt gggtattgct attgttagta cttctaaagg tgtaatgact
     7021 gatcgtaaag cacgttctca aggtgttggt ggtgagttgt tatgcattgt agcctagtgg
     7081 aggaaaagaa atgtcacgtg tcgcaaaaaa cccagtgact gttcccgctg gtgtagaagt
     7141 aaaatttgga acagaggcat tagttattaa gggtaagaac ggtgaattgt cttttccttt
     7201 gcattctgat gtagccattg aatttaatga tggcaaattg gcttttgttg cgaataacag
     7261 cagtaaacaa gcaaatgcaa tgtctggtac tgctcgcgca ttagtcagca atatggttaa
     7321 aggtgtttca gaaggttttg agaaaagatt gcaattgata ggtgtgggtt atcgtgctca
     7381 agcacaaggt aaaatcttga atctgtcttt gggtttttct catccgatcg tatatgaaat
     7441 gcctgaaggt gtctccgttc aaactcctag ccaaacagag attgttttaa ccggctcgga
     7501 taaacaagtt gttggtcaag ttgctgctga gattcgtgcg ttccgtgctc ctgagcctta
     7561 taaaggtaaa ggtgttcgct atgtaggaga agtagtggta atgaaagaag ccaagaaaaa
     7621 ataattgagg ttcactaatg gataaacata caacccgact ccgtcgtgca cgcaaaaccc
     7681 gtgctcgtat tgcggacttg aaaatggtaa gattatgtgt gttccgaagc aataatcata
     7741 tttatgctca agtaattagt gctgaaggtg ataaagtatt ggctcaagcc tctacattgg
     7801 aagctgaggt gcgcggtagt ctgaaatctg gaagcaatgt tgaagcagct gcaatagttg
     7861 gtaaacgtat cgctgagaag gctaaagcag caggtgtaga aaaggttgct tttgatcgtt
     7921 caggtttcca atatcacggt cgtgtgaagg ctttggctga agctgctcgt gaaaatggtt
     7981 taagcttcta aatatttgga gactttcaga tggcaaaaca tgaaattgaa gaacgcggtg
     8041 acggtctgat tgaaaagatg gtcgcagtta accgtgtaac caaagtagtt aaaggtggtc
     8101 gcattatggc tttctctgcg ctaactgttg ttggtgatgg agatggtcgt attggtatgg
     8161 gtaaaggtaa gtcaaaagaa gtgcctgttg cagtccaaaa ggcgatggat caagcacgac
     8221 gctctatgat taaggtacca ttaaaaaatg gtacgattca tcatgaggtt attggtcgac
     8281 atggtgctac taaagtattt atgcagcctg ctaaagaggg tagtggcgta aaagccggtg
     8341 gacctatgcg tttggttttt gatgctatgg gcattcataa tatctccgcc aaagtgcacg
     8401 gatctactaa cccatataat atcgtacgtg caacattaga tggtttgtct aagttgcata
     8461 ctcctgctga tatcgcagcc aaacgtggct tgacagtgga agacattttg ggagttaacc
     8521 atggctgaac aaaaaaagat tagggttaca ttggttaaaa gcctgattgg tacaattgaa
     8581 tctcatcgtg catgtgcacg cggtttaggt ttgcgtcgtc gcgagcatac ggtagaggtt
     8641 ttagataccc ctgaaaaccg tggtatgatt aataaaatca gctacttgtt gaaagtggag
     8701 tcttgatatg tttttgaata caattcaacc tgctgttggt gctacgcatg ctggtcgtcg
     8761 tgttggacgc ggtattggta gtggtcttgg caaaacgggt ggtcgtggtc ataaaggtca
     8821 aaagagccgg tctggtgggt ttcataaggt gggtttcgag ggtggtcaaa tgcccttgca
     8881 acgacgcctc cctaaaagag gttttaaatc tttaacagca tcagctaatg cacagcttcg
     8941 tttaagtgaa ctggaatcaa ttgctgttaa tgagattgat attttggtct taaagcaagc
     9001 gggtctgatt gcatctacag tctctaatgt taaagttatt gcttctggtg aaatttctaa
     9061 ggcagttgct ttgaagggta ttaaagttac caaaggtgcg agagctgcta tcgaggctgt
     9121 tggtggtaag attgaaatgt aaggtttaat attgtggcta atcaacaaac gtcatcaggt
     9181 tcatccaaat ttggagatct taagaaacgt cttttgtttc tatttggagc attgattgtt
     9241 tttcgaattg gtgcccatat acccgtacct ggagttgatg ctgttgcttt agctaaatta
     9301 tacgaaagcg ctggaaacgg catcctggga atattgaata tgttttccgg tgggtcgtta
     9361 gagcgcttta gtatatttgc aataggaatt atgccatata tttcagcttc tattattgta
     9421 cagctcgctt ctgaaatttt gccatcattg aaggctttaa aaaaagaagg ggaggctggt
     9481 agaaaggtaa ttacgaaata tactaggtat ggtactgttt tgttagcaat tcttcaaagt
     9541 ctaggtgttg catctttcgt atttcagcaa ggaattgttg taacaagttc atttgagttt
     9601 catgtttcca cggtagtttc tttggtaacg ggaaccatgt ttcttatgtg gcttggggag
     9661 caaattactg aaaggggtat cgggaacggt atttctttaa tcattacggc aggtattgct
     9721 tcaggtattc cttcgggtat tgcaaagctg gttacactga cgaaccaagg ttctatgagc
     9781 atgcttacgg cgttgtttat tgtatttggt gccttattat taatttattt ggttgtatac
     9841 tttgaaagtg cacagcggaa gattcctatt cattatgcaa aacgccagtt taatggtagg
     9901 gcgggtagtc aaaatacgca tatgcctttc aagttgaata tggctggtgt tattccccca
     9961 atttttgctt ccagtattat tctatttcca tctactcttt taggttggtt tggttcggct
    10021 gatacaaata gtgttttgca caaaatagct ggattgttac aacacggtca attgctgtat
    10081 atggctttat ttgcagcgac agttattttc ttttgttatt tttatacggc tttggttttt
    10141 agccctaaag aaatggcaga gaatttaaaa aagagtggtg cttttgttcc tgggattaga
    10201 cctggtgagc agacctctag gtatttagaa aaagttgtat tacgtttgac attgtttgga
    10261 gctctttata ttacaactat ttgtttaatt ccagagttct taactacggt tttaaatgta
    10321 cctttttatt tgggtggcac gtctttgttg attctagttg ttgtaacgat ggattttagt
    10381 acacaaataa attcgtatag gcttactcaa cagtatgata agttaatgac tcgttcagaa
    10441 atgaaatcat tttctcggaa atagaattat ggcgaaagaa gatactatcc aaatgcaagg
    10501 tgaaattctt gaaactttac ctaatgcaac atttaaagta aaacttgaga atgaccatat
    10561 tgtattgggt catatttctg ggaagatgcg gatgcattac attcgtattt ctccgggaga
    10621 taaggtcaca gtagagctga caccttatga tctaactagg gctcgaatcg ttttcagagc
    10681 aagataaacc aataaaagga aaataaaatg cgtgtacaac catctgttaa gaaaatttgc
    10741 cgaaattgca agattattcg tcgaaatcgt gtagttcgtg taatttgtac tgatctccgt
    10801 cacaaacagc gtcaaggtta atggaatatt tcttttaatg tgattctgtg atatagtgac
    10861 acactttgcc ctaaaaagga aaaaatatgg ctcgtattgc aggggtaaat atccctaata
    10921 acgcacacat cgtaattggt cttcaggcta tttacggtat tggtgctact cgtgctaaat
    10981 tgatttgtga ggctgcaaat attgcgcctg atactaaagc aaaagatttg gacgagactc
    11041 aattagatgc tttgcgtgac caagttgcca agtatgaagt agaaggtgat ttgcgtcgtg
    11101 aggtaactat gagtatcaag cgattgatgg acatgggctg ctatcgtggc ttccgtcatc
    11161 gtcgcggctt accatgccgc ggtcaacgca ctcgtacaaa tgcgcgtacc cgcaaaggtc
    11221 cgcgtaaagc gattgctggt aagaaataaa ttttaaggaa ttttattaat ggctaaagca
    11281 aacacagctt cacgtgtacg taaaaaagta cgtaaaaccg tgagtgaggg tattgtgcac
    11341 gttcatgcat ctttcaacaa taccatcatt acaatcactg accgtcaagg caatgcgttg
    11401 tcttgggcta cctctggcgg cgctggtttt aaaggttctc gtaaaagtac accatttgca
    11461 gcacaagttg cagcagaagc agctggtaaa gttgcccaag agtatggcgt taaaaattta
    11521 gaggttcgta ttaaaggtcc aggtccaggt cgtgaatcct ctgtacgtgc tttgaatgct
    11581 cttggtttca agattaccag cattactgac gttaccccgt tgcctcataa cggttgccgt
    11641 ccgcctaaaa aacgtcgtat ttaatattgg agtgatttga aacatggcac gttatattgg
    11701 ccctaaatgt aagttggcac gtcgcgaagg tacggatttg tttttgaaga gtgcgcgccg
    11761 ctctttggat tctaaatgta aaattgattc cgctcccggt cagcatggtg caaaaaaacc
    11821 gcgtttgtca gactatggtt tgcagttgcg tgaaaaacaa aaaatccgcc gtatttatgg
    11881 cgtattagaa cgtcagttcc gtcgttattt cgcagaagct gatcgtcgta aaggttctac
    11941 cggcgagttg ctgttgcagt tgctggaatc tcgtttggat aatgtcgttt atcgtatggg
    12001 tttcggttct acccgagctg aagcaagaca gcttgtttct cataaggcga tagttgtgaa
    12061 tggacaagtt gtcaatattc cttctttcca agtgaaagct ggtgatgttg tctcagttcg
    12121 tgaaaaagcc aaaaaacagg tacgtattca agaagcattg ggtttggcaa ctcaaatcgg
    12181 cttgccgggt tgggtttctg tagatgcgga taaacttgag ggtgtgttca aaaacatgcc
    12241 ggatcgctcg gaattgaccg gtgatattaa tgaacagctg gtggtagagt tctactctaa
    12301 ataatgctag ctcagtgagg gacagttaaa tgcagaatag cacaaccgaa tttttgaaac
    12361 ctcgtcaaat tgatgtaaat actttttctg caactcgtgc aaaagtatct atgcagccat
    12421 ttgaacgtgg tttcggtcat accttaggta atgctttgcg ccgtatctta ctgtcatcca
    12481 tgaatggttt tgctcctact gaagtagcta ttgccggtgt attacacgaa tattctactg
    12541 ttgatggtat tcaggaagat gttgttgaca ttttgctgaa tattaaaggt attgtgttta
    12601 aactccatgg tcgtagccaa gttcaacttg tgttgaagaa atcaggttca ggtgtcgtat
    12661 ctgccggtga tattgagttg ccgcatgatg tagaaattct gaatcctggt catgtcattt
    12721 gtcatttggc tgataacggt caaattgaga tggaaattaa agtagagcaa ggtcgtggtt
    12781 atcaatctgt ttcaggtcgt caggtagttc gtgatgagaa ccgtcagatt ggtgcaatcc
    12841 agttggatgc gagcttttcg cccatcagcc gtgttagctt tgaggttgaa cctgcacgtg
    12901 tagagcagcg gacggatctt gataagttgg ttttggatat cgaaaccgac ggttctattg
    12961 atcctgagga agctgtacgc agtgcggcac gtattttgat tgatcagatg tctatttttg
    13021 ctgatttgca gggtacgcct gtggaggagg ttgaagaaaa agcacctcct atcgaccctg
    13081 ttcttttgcg tccggtggat gatctggaat tgacagtacg ttcagctaat tgtttgaaag
    13141 ctgaggatat ttattatatt ggcgatttga ttcaacgcac tgaaaccgag cttcttaaaa
    13201 cgccgaattt gggacgtaaa tctttgaatg agattaagga agtattggca tctaaaggtt
    13261 tgacactggg ttctaagttg gaagcatggc cacctgtagg cttggaaaag ccttaatgaa
    13321 gaattaaagg ataattgata tgcgtcatcg taatggcaat cgcaaattaa accgtaccag
    13381 cagtcatcgt gctgcaatgc tgcgtaatat ggcgaattca ttattgactc acgaaactat
    13441 tgtaacaact ctgcctaagg ccaaggaatt gcgccgtgta gtagagccgt tgattacatt
    13501 gggtaaaaag ccgtcattgg caaaccgccg tttggcattt gaccgtactc gcgaccgtga
    13561 tgttgtagta aaactgtttg gcgatttggg tcctcgtttt actgctcgta acggtggtta
    13621 tgttcgggtg ttgaaatacg gattccgtaa aggtgataat gcacctctgg cactggttga
    13681 attggttgac aaaccggctg ctgagtaatt ttagtcatat aacgccatct gccgaaaagc
    13741 aggtggcgtt atttttgcaa tatctgatag gtaatagggt attggctatc atgtttaaaa
    13801 tattaattga atagctaagg tttgcgcggt aaacttacat cattaaaaaa ttctatgatg
    13861 gtttatataa tgaatgcctt cgatataaag tcgacaaaga tggacgtatt gtctatatct
    13921 ttgcatacat cagacttgtt tgatttggaa gatgtgctgg tcaaattggg caagaagttt
    13981 caagagtctg gtgttgttcc atttgttctg gatgttcaag agtttgatta tcccgagtct
    14041 ttggatcttg ctgcattggt ttcgttgttt tcaaggcatg gtatgcaaat tttgggtctg
    14101 aagcattctg atgaacgttg ggctgctgcg gctatgaagt atcatttgct gttttgtctg
    14161 tctcattcgg aaaatgttaa agaactgggt caggttgagg tgcagaaaac ggaggatggt
    14221 cagaaagcaa ggaaaacagt attgattaca tcccctgtcc gtaccggtca gcaggtttat
    14281 gccgaagatg gcgatttgat tgttacgggg gcggtcagcc agggggcaga attgattgcg
    14341 gatggcaata tacatattta tgcgccgatg agggggcgtg ctttggccgg cgccaagggt
    14401 gatacttctg cccgcatatt tatccactcc atgcaggcag aactggtttc tgtggcgggt
    14461 atttaccgta attttgaaca ggatttgccg gaccatctgc acaagcagcc ggtacagata
    14521 ttgttgcagg ataaccgatt ggttatcagt gcaattggct cagagtaatt gtttgatatt
    14581 ttaaaaagga aatattgtgg caaaaattat tgtagtaact tcaggtaagg gcggtgtcgg
    14641 taaaacgact accagtgcca gtattgcgac aggtttggca ttacgcggat ataaaactgc
    14701 ggtaattgat tttgatgtgg gtttgcgtaa cctcgacctc attatgggtt gcgagcgtcg
    14761 tgtcgtttat gacctgatca atgtcattca gggggaggcg acgctcaacc aggctttgat
    14821 taaagataaa aattgtgaaa acctgtttat tttgccggct tcccagactc gggataaaga
    14881 cgctttgaca cgcgagggcg tagaaaaagt gatgcaggag ctgtccggca agaaaatggg
    14941 ctttgagtat attatttgcg actctcctgc cggtattgag cagggtgcat tgatggcgtt
    15001 gtattttgct gatgaagcca ttgtaacgac caatcccgag gtttccagtg tgcgtgactc
    15061 cgacaggatt ttgggaattt tgcaaagcaa atcccataag gcagagcaag gcggttcggt
    15121 taaagaacat ctgttgatta cgcgttattc tcccgaacgt gtggcaaaag gcgaaatgct
    15181 gtctgtacag gatatttgcg atattctgca tattcctttg ctgggtgtga ttcccgaatc
    15241 ccaaaacgtc ttgcaggcat ccaattccgg agaaccggtc atccatcagg acagcgtggc
    15301 ggcttccgag gcatataagg acgttattgc ccgtcttttg ggcgagaacc gtgaaatgcg
    15361 tttcttggaa gctgagaaaa aaagcttctt caaacgtctg ttcggaggat gaggtatgtc
    15421 attaatcgaa cttttattcg gcagaaagca gaaaacggca accgttgccc gcgaccgcct
    15481 tcaaatcatc attgcccaag agcgcgccca agaaggtcag gctccggatt acctgccgac
    15541 tttacgtaaa gagttgatgg aagtcctgtc caaatatgtg gatgtttcat tagacaatat
    15601 ccgtatttcc caggaaaaac aggatggtat ggatgtgctt gagttgaaca ttactttgcc
    15661 ggaacagaaa aaggtatagg acatgacctt aaccgaattg cggtacatcg tcgcagtcgc
    15721 ccaagaacgt catttcggca gggcggcgcg gcgttgtttt gtcagccagc ccactttgtc
    15781 tattgccatt aagaaattgg aagaagagct tgccgtctct ttgtttgacc ggagcagcaa
    15841 cgatattatt acgaccgagg cgggggaacg tatcgttgca caggcgcgta aggtgttgga
    15901 agaggcagag cttatcaggc atttggcaaa tgaagaacaa aacgagctgg agggtgcgtt
    15961 caaactcggg ctgattttta cggttgcgcc gtacctgctg ccgaaactga ttgtctcgtt
    16021 gcgccgtact gcaccgaaaa tgcctttgat gttggaagag aattacacgc atactttgac
    16081 cgagtcgctc aaacgcgggg acgttgacgc gattatcgtt gccgaaccgt ttcaagagcc
    16141 gggcattgtt accgaaccct tgtatgacga accgtttttc gtgattgtcc cgaaagggca
    16201 ttcatttgag gaactggatg ccgtttcgcc acggatgctg ggtgaggagc aggttttgct
    16261 gctgacggaa ggcaactgta tgcgggatca ggtactctca agctgttccg aattggcggc
    16321 gaaacaacgc atacaggggt tgaccaatac attgcagggc agctcgatca atacaatccg
    16381 ccatatggtc gccagcggtt tggcaatcag cgtgttaccg gcaaccgcgc tgaccgaaaa
    16441 cgatcatatg ctgttcagca ttattccgtt tgaaggtacg ccgccaagcc ggcgggtcgt
    16501 attggcgtac cgccgcaatt ttgtccgtcc gaaggcgttg tcggcgatga aggcggcgat
    16561 tatgcagtcg cagcttcacg gggtaagttt tatccgcgac taggcgcagg cattgttttc
    16621 aaaacgccat ttccctgagc cgacaacacg gtatgccaag atattgccgt catcatcgat
    16681 tttgagtata gcatcgccac ggaaactgcc gtcctgaaga tattcgactt ttgcatcact
    16741 gtgaatgttt tcatcagtgc cgatgcaatg ccatgtatag tggattaaca aaaaccagta
    16801 cggcgttgcc tcaccttgcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg
    16861 atttttgtta atccactata aaagaggccg tctgaaaaac atttttcaga cggccttgtt
    16921 gattcaatca aatcagtctt tcaacttcga caactgagtt tgcacttttg ccattttgtc
    16981 ttccaattcc gccaaatcgg ctttgtcttt ttccaccaaa tgcgccgggg ctttttcggt
    17041 gtagccgggt ttggagagtt tggcgttgag tttgtccaag gctttttgca gcttctcggc
    17101 ttctttgctc aaacgggcgg tttcggcagc tttgtcgatt tcgactttca acatcaggcg
    17161 cgcgccgttg cagacggcga cgggcgcgtc ttcgctttcg ggtagggcgg cgacttgctg
    17221 tgcttcggtc aggcgggtca tcatcggcag gtatttgagg tagtccgcca agtcgtctgc
    17281 gctttcgaca aacagcgggg ctttcacgtt gggctggatg cccatttcgc cgcgcaggtt
    17341 gcggactgcg ccgatcaaat cctgcaacac ggtcatttgc ccgaatgccg tctgaacaat
    17401 ctcgccgccg tcggcttcgg ggaagcgggc gagcatgatg ctgtcggcgg ttttcgcgtc
    17461 gcacataggg gcgacggttt gccacagttc ttcggtgatg aacgggataa tcgggtgcag
    17521 caggcgcagg gcggcttcga gtacgcgcaa taaggtatgg cgtgtggcgc gttggcggct
    17581 ggcgcagccg gtttgaagct gcactttggc aagttccaaa taccagtcgc aatagtcgtt
    17641 ccacacgaag ctgtacaggg tttccgccgc caaatcaaag cggtaggttt cgtaggcttg
    17701 cgtaacctgc tcgatggtct gattcagacg gcctacaatc cacatatcgg ggaaggaata
    17761 gccgcgcggt tcggtggcgg ttgcgccgta gccgcagtct tggttttcgg tgttcatcaa
    17821 gacgaagttg gtggcgttcc agattttgtt gcagaagttg cggtagcctt cggcgcgttt
    17881 gaagtcgaag ttcacgctgc gccccaagct ggcgtagctc gccatggtga aacgcaatgc
    17941 gtccgcgccc atactcggaa tgccttcggg aaagagtttt ttggtggctt cttccacttt
    18001 cggcgcggtt tcgggtttgc gcagaccggt ggtacgtttc accagcagtt tttccaagtc
    18061 gatgccgtcg atcaaatcca cagggtcaat gacgttgcct tcggatttgg acattttttt
    18121 gccttcgtgg tcgcgcacga tgccgtggat gtacacgtct ttaaacggta ctttgccggt
    18181 aaagtgggtg gtcatcataa tcatgcgcgc cacccagaag aagatgattt cgtagccggt
    18241 gaccaagacg ttggacggca ggaaggcttt gagttcgtcg gtttcagacg gccagccgag
    18301 cgtggagaac ggcacgagtg cggaggagaa ccatgtgtcc aatacgtctt cttctcgggt
    18361 caagcctgtt ttgccggctt gtttttcggc ttcttcctga ttgcgggcaa cgtacacatt
    18421 gccttcgttg tcgtaccatg cagggatttg atgcccccac cacagttggc gcgagatgca
    18481 ccagtcttgg atgttgttca tccattggtt gtaggtgttg acccagtttt cggggataaa
    18541 gcgcaccgcg ccgctgtcga cggctttttt ggctttgtca gcaaggctta agcctttgaa
    18601 ttcgctgtca ggctcgccgc cgtttggcgt ggcggacatg gcgacaaacc attggctggt
    18661 cagcatcggc tcaatcacgg aaccggtacg gtcgcctttc ggcgtcatca gcgtgtgcgc
    18721 tttgatttcg accaagaaac cttgttcctg caaatcggca accatttgtt tgcgcgcggc
    18781 aaagcggtcc aagcctgcgt atttttcagg cagggcaaag cttggttgcg cttcgccttt
    18841 gaagttgaac acttcggcgt ttgccaatac tttggcttcc aagtcgaaca cattaaccaa
    18901 gcgcgtatcg tggcgtttgc cgacttcgta gtcgttgaag tcgtgcgcag gcgtgatttt
    18961 cacgcagccg gtgccgaagt ctttttcaac gtattcgtcg gcaatcacgg gaatggtgcg
    19021 gccggtcagc ggcaggatta attctttgcc gattaagtgg gtataacgtt cgtcttcggg
    19081 attgacggca acggcaacgt cgcccaacag cgtttcggga cgggtggtgg cgacgatgac
    19141 ggcttcggca ggattgtccg ccagcggata gcggatgtgc cacatagagc cttgttcttc
    19201 cacgctttcc acttccaaat cagacactgc ggtacccaat accggatccc agttcaccaa
    19261 gcgtttgccg cgataaatca agccctgttc atacaggcgt acgaacactt cggttacggt
    19321 ttcggcgcgt acatcgtcca ttgtgaaata ctcgcgcgtc cagtcggcag aacagcccac
    19381 acggcgcatc tgctgcgtaa tcgtgccgcc ggaaacttct ttccattccc acactttttc
    19441 caagaatttt tcacgaccca agtcatgacg ggacacgttt tgcgcggcaa gctgacgctc
    19501 gaccacgatt tgcgtggcga tgcccgcgtg gtcggtgccg ggaatccagg cggtgttgca
    19561 gcctttcatg cggtagtagc gggtcagacc gtccataatg gtttggttga aggtatggcc
    19621 catgtgcagc gtgccggtta cgttgggcgg cggcagttgg atggaaaaag acggtttggt
    19681 caaatccata tcgggctgga aataaccttg gctttcccag ttttgatagt gtttggattc
    19741 gatttcggcg ggggagtatt tgtctaacat gatggaactt tgtgaaatta aggttatttt
    19801 tgatgtgcgg attataacgc aaaaaggccg tctgaatcat ttcagacggc ctttggcata
    19861 cgggttttaa aaatggaaca ataccaggct gacggcaatc accgccatac ccgttgtcag
    19921 gccgtaaacg gtttcatggc cgtctgaata gcgtttggcg gccggcagca gctcgtccaa
    19981 cgccaaaaac accatcacac cggctatcac gccgaatacc gaaccaaaca cggcaggcga
    20041 caaaaacggc tgcaaaacca aatagcccaa agccgccccc aacggctcgg ccaagccgga
    20101 tagcagacac gcccacaccg ttttcttacg gctgcgggtg gcaaaataaa ccggcgcggc
    20161 gatggaaatg ccctccggaa tattatggat ggcaatcgcc aaggccaaag gcatcccgac
    20221 cgccggattt tccaatgtgg caaaaaacgt cgccaagcct tcggggaaat tgtgcgcagt
    20281 aatcgcaaac gccgccatca tgccgactcg cgcgatatgg cggcgtttgc tttcttgaaa
    20341 cgacgggtct tgcgcgtcta aagtttcatg cgggttcggc accagacggt caatcagcgc
    20401 aatgccgccc atcccggcca aaaatgccat ggtcgccgcc gcaaacgcgt ggtctttatc
    20461 ataaatttca gcgaacgcct cgctggactt actgaaaatc tccgtcaggg aaacatacac
    20521 cattgcaccg ccggcaaatg ccaaaccaaa cgacaacaca cgcggattgg gcgttttgga
    20581 aaacattacc aagccgctgc ctaatacggt aaacaaaccg gcagccaacg tgatggaaaa
    20641 ggcaacggcc aaattggaca tcgaaaaatc gggcatgaga aaacctgcgc taaaagcggg
    20701 gacaggttca gactaacact ttttaatgcg tatgataata gttattattt attttattgg
    20761 ttggatacac ggattttgaa acaaaaggcc gtctgaaaaa tagttttcag acggccttta
    20821 catttgaaat gccactaaac cttatcgctt tccagcttaa gcctgatagc gcgacaggct
    20881 caaatcgtcg ctgcggattt cggtgtcttt gccgctcacg atatcggcgg ttaattttgc
    20941 cgaacccagc gacatggtcc agcctaaagt accgtggccg gtattcagaa acaggttgtc
    21001 aaagtgggtg cgaccgatta acggcgtgct gtcgggcgtc atcggtctga ggccgctcca
    21061 gaacgatgct tggctcaaat cgccgccttc cgggaacaag tcgttgacga ccaaagccaa
    21121 ggtttcgcgg cgtttttcgg gcagtttgat ttcgtagccc gacaattctg ccatgccgcc
    21181 gacgcggatt ctgttgtcaa agcgcgtgat agcgactttg tagctttcat ccaaaacggt
    21241 ggacaccggc gcgccgtctg aattggtgac cggcagggtc aaggaatagc ctttgacggg
    21301 ataaatcggc agatcgaggt ctaactgcgc caacacagtc ctactgaagc agccgagcgc
    21361 acagacaacg gcatctgctt caaaccgccc tgttttggtt tcaacggctt tgatgcgcag
    21421 cccgttatgg tcgatgcggc tgatggtttg gttgaaatga aactgtacgc ccttttcctg
    21481 acacaatttg tataggtttt cggtgaagag gtggcagtcg ccggtcgcat ctgcaggcag
    21541 gtgcaggccg ccggcaattt tggcggtaac gcgtgccagc gcaggctcaa attctgcaca
    21601 ttcttcgggt ttcagacggc ggtacggcac gccgtagcgt tccaaaacgg caatgtcttg
    21661 ttctgccgct tcaacttctt tagcctgacg gaaaatctgc aacgtccctt ttttgcgttc
    21721 ctcaaaattc atgccggttt gcgcttcaaa acggcggaac atttcacggc tatattcgga
    21781 aatcctgacc atgcgctctt tattggtttg atagtgcgct gccgtgcagt tttgcagcat
    21841 ttgccacagc cattcgattt gatacaggct gccgtcgggg cgaaacagca aaggcggatg
    21901 gcttttaaac agccatttca gtgctttggt cggaataccg ggtgcagccc aaggcgtggt
    21961 atagccgtaa gaaagctggc ctgcattggc aaaactggtt tccatcgcca caccctcggc
    22021 gcggtcgatg accgttactt catgtcctgc ctctgccaga taccacgcgg aagacacgcc
    22081 ggcaacaccc gcacctaaaa caagcacttt catgtttctc cctccggctt tttcaaaaca
    22141 gacttaatat gccgtgccgt ctgaatattc ggattcagac ggcctcggat attaatgcgg
    22201 caattcgcca tttgtgattt tttgtttgaa gtcgcgcgtt tcattgacga tgactttcgc
    22261 catcaataaa agtgcaatca ggttgggcaa tgccatcaag ccgttgaatg tgtccgaagc
    22321 cagccacacc aaatcaaggc tcaacacggt acccagcata acggaagaaa cataaccgac
    22381 gcggtacaaa ccggcaaatt tctcgccgaa aacatacacc gcgcattttt cgccgtaata
    22441 gcaccagccg agaatggttg agtaggcaaa gaaaatcagg ccgatggtaa caatccagcc
    22501 gccgatgccg ggcagcattt tttggaatgt gacggttgtc agtgccgcgc cgctcacttc
    22561 aggtttgaca aactcgccgc ccgcgccgag cagtcccatt accaacacga tgccggtaat
    22621 cgagcaaacg acgatggtat ccaaaaacgt accggtcata gaaaccaagg cctgacggac
    22681 gggatggtcg gttttcgcgg ctgcggcggc aataggcgca gaacccatac ccgcctcatt
    22741 ggagaacacg ccgcgcgcca cgccgtagcg gatgaccgta ccgatagcgc cgcccgccac
    22801 tgcctgcgcg ctgaacgcat cggagaaaat cagcttgacg gcaggcatca gtgcatcgga
    22861 attaatcgcg ataatggaaa gaccgcccaa cacataaaac accgccatag caggcacgat
    22921 gaaagaagcg gctttggcga tgcctttaat accacctaaa acgacaacgg cagtcagaac
    22981 ggtcaacgta atgccggtat aggcaggttc gataccgaag ctggtttgca ccgcctgtgc
    23041 aaccgagttg gactgcaccg agctgccgat accgaaggaa gcgaatgtgc cgaacagcgc
    23101 aaatgcaacg gccatccatt tccagttttt gcccaagcct ttttcgatgt aatacatcgg
    23161 gccgccggac atttcgcctt tggaattgtt gacgcggtat ttcaccgcca acacgccctc
    23221 gccgtatttg gtggccatac cgaaaatggc ggttatccac atccaaaata ccgcgcccgg
    23281 gccgccggtt accaccgcag tcgccacgcc ggcgatgttg cccgtaccga tggtggcgga
    23341 cagcgcggtc atcaacgccg caaaatggga aatatcgcct tcgtggcctt cgccgccttc
    23401 atacttcttt gacggcacaa acgcctgttt cagcgcatag cccaacatag tgaactgcaa
    23461 gcctttcagc aaaacggtca gcacaatgcc cgtgccgacc agcagcatca acataacaga
    23521 tccccagacc cagccgctga cggtttcgaa aaaggctttg agattgtcta aaaacaattg
    23581 catggctttc tcctttgtct gttttatttt taaaacacca cttttgtagt gtcctataat
    23641 ttcagcacag aatattcaat aagacaatat gttcttttga aaaatacttt tggttttttc
    23701 gccgaaagca ggtcggttca agctgcggaa attgtttgca attctttaaa agcagcggcg
    23761 gaggtcacaa tgaaatgtcc gaatggggat gtggcgggcg gcagaaatca tcaatgctgc
    23821 cgactgccat acttctgaaa tctacaaaat gatgcatcga tcaaacaata taccgcttta
    23881 aaaaaaccga tgccgtctga aacgctttcg gggcttcaga cggcatcaaa aggatacggt
    23941 cagcggatga tgccgcgcgt cgattgtgcg aaaaagtctc ggaatacggc aagctcggct
    24001 tgggtttcgg cgcggcggag gatgtctgcc ttggcctctt caaacggaat gccgcgatgg
    24061 tagagggttt tgtacacgtt tttgacggcg gaaatctgct ctgcggtaaa accgttgcgg
    24121 cgcatgcctt cgctgttgag ccccgccggt tcggcgcggt agcccgatgc cataaagtag
    24181 ggcggcacgt ctttgtgtac gcctgcggca aacgcggtca tggcgtagtc gccgatgcgg
    24241 cagaattgga aaaccagcgt gtagccgccc aaaacgacgt agtcgccgac ggtaacgtgt
    24301 ccggcaagcg aggcgttgtt ggcgaagatg gtgtggttgc cgaccacgca gtcgtgcgcg
    24361 aggtggcagt acgccataat ccagttgtcg tcgccgatac gggtttcgcc gatgccggtt
    24421 accgtgccta aattaaaggt ggtgaattcg cggatggtgt taccgttgcc gataatcagc
    24481 ttggtcggct cgtcgcggta ttttttgtcc tgcggaattt cgccgaggct ggcaaattgg
    24541 aaaatgcggt tgttttcgcc gatgctggtg tggccgttga tgacggcgtg cggaccgatt
    24601 tcggtattcg caccgatttg gacgttgggg ccgataacgg tgtacgcgcc gactttgacg
    24661 ccggagtcga gttcggcttt ggggtcgatg acggcggtcg ggtggatgag ggtcatgttt
    24721 tgaatactcc gctgcttgtt aagacaaata atagaaaatt acagtttgtc gatttcatcg
    24781 cagaccgcaa tcacttgatc gctaaaatgg ctaaatactg aagcagtatc tacatccatg
    24841 taattaagca gttcaaaaat agattggtga atatttctag cgtagttggt atcttttatt
    24901 gcacctttaa aacaaatcgg ctcgtgatgt gccaaacgat tacggaattt gttaatattg
    24961 gaaagttctc gaaaaatcca tttttggtta tattgcacac ttggtgtaga tttgggcttt
    25021 tttggaaaaa ctttcatcaa tactttccct gtaacatcaa attgggcatc tttccctcct
    25081 gcaaacagat accgccaaaa accgaatcct agtcctgcaa ccaattggtt gtggtcaaac
    25141 tttcccccgc tattattttg tattttagat aatgcaacct tgattaattc agcagattct
    25201 ttcgtgccat ttcttaaaaa gcaaccttga tattttaatg cgggattcgt ctgtggctgt
    25261 atgctgttat ataaccagtt tctgtcttta aacgcctgct gaaggcaaat gtcaattttg
    25321 tttcttaaaa caatctcaaa tattgaaaca acatgaaaaa gttctcttga taaagataaa
    25381 ttcaataaat acaattcagc tgctttgtct tccccaacgg catttttata ccgttccatt
    25441 ctgaaatcag aaaaaatact acagcattcc ttataatcca cgctaacacc tgattcttta
    25501 tttttaaatt gacgtttcaa actatatttg atacagttag gtaaaactaa tccccaccgt
    25561 gtccctgggt ttgaataaac ctaaaccggt gggttttctt tttattaggt cgtctgaaac
    25621 tttcagacga cctttttctg aacactcaaa ccacgcgttt ggcacacatg atgatggctt
    25681 cgacggcgac ttgcccgtcc actttggcaa cggcgttgaa tttgccgatg ccgcgccggc
    25741 tggtcagcag ctcgacttca aagacgagtt ggtcgccggg gatgacttgg cgtttgaagc
    25801 gggcttcgtc tatgccggcg aagaagaaga attcgttttc tttgcgcccg ccctcgctca
    25861 aaatcgccaa cgtgccgcac gcctgcgcca tcgcttcgat gatgagtacg ccgggcatca
    25921 cgggcaggtc gggaaagtgg ccttggaact gcggttcgtt tatggttacg tttttgatgg
    25981 cggtcagggt tttcatcggc tcgaaggcgg tgatgcggtc gagctggaga aacggatagc
    26041 ggtgggggat gagtttttgg atgtctttgg cttcgatggg gagttgtacg tccatgttcg
    26101 tcgtattcct tgaataaagt cggtttggtt atttgctgtc ttgaccggca tctgaaagct
    26161 gctgctccag tgttttgagg cgtttgttca tttcgcttaa gcggtggatg taaacggcgt
    26221 tgcgcgccca ttctttatgg gtggacatcg ggaagatgcc ggcgaggtgt ttgccgcttt
    26281 cggtaatgct gtgggtgacg gacgtgccgc cgccgatggt ggttttgtcg gcgatttcga
    26341 tgtgtccgac cgtaccgacc ccgccgccga tgatgcagta gctgcctatg gttacgctgc
    26401 cggagatgcc ggttttggcg gcgatgacgg tgtgcgaacc gattttgcag ttgtgcccga
    26461 tttggacttg gttgtcgatt ttggtgccgt tgccgacggt ggtgtcgctc atcgcgccgc
    26521 ggtcgatgtt ggtgttcgag ccgatttcta cgtcgtcgcc cagcgttacc gcgccggttt
    26581 gcgggatttt gaaccacgaa tcgccggcaa aggcgagtcc gaaaccgtcc gcgccgatga
    26641 ccgcgccgct gtggatttcg acgcgtctgc cgagtgtgca gccgtaataa acgacggcgt
    26701 tgggatgcag gacgacttcg tcgcccagtc tgcaatcgtg ttggacgacg gcgtttgcca
    26761 agatgcggca gccttcgccg agtacggtgt tcgcgccgat gtaggcgttc gcaccgattt
    26821 cgcagcttgc ggggacggtc gcgcccggtt cgacgacggc ggtcggatgg atgccgccgc
    26881 gcgctttgac gacgggtgaa aacaggcgcg cgactttggc gaaatagaga taggggtcgt
    26941 cggcgacaat caggttgcgc ccttcaaatc cgtctgccgc tttggcggaa acgatgaccg
    27001 cgcccgcgct gctgtcgtgg acttcggctt tgtatttcgg attggcaagg aagctgatgt
    27061 gttccgcctg cgcgtctgcg agcgggcgca cggcggtaac ggaaatgtcc tcgccgcgcc
    27121 attcgccgcc gagccgcgcg gtgatttggg acagggtgta ggtggccgga atcatggttt
    27181 tcctgttcgg tatgccgtct gaaagggtca gcgggcgttc atttctttaa tgacgctgtc
    27241 ggtaacgtcg tattgggtgt tgacgtaaat cacgttctgc aaaatgacat cgtaaccttc
    27301 ctgtttggcg attttgacga tgacgcggtt ggcgttttgc tggagggagg caaactcttc
    27361 gttgcggcgg aggttgtagt cttcttcaaa ctgcgcctgt tttttgcgga acgctgcgac
    27421 cagcccgcgc catttttctt cggcttgcac cttttttgcg tttctgagtt tgccttcggc
    27481 aagctgcctt tccaaatcca gaccttcgcg ttgcagtttt tgcaattcgt cctgacgagc
    27541 ggaaaattcg ctgtccagcg ttttttgaat cttgcgcgcc tgcttggatt cgaggtagat
    27601 gcgctcggtg ttgataaagc cgattttttg gaaggtgtcg gcgtgcgcgc ctgcggtgca
    27661 gcacaaaccg atcagagccg cggcaaacgc gcgggtcaaa cgggtcatgg taaaactcct
    27721 tcgaatgttg ccgcgaaatg ccgtctgaag ggcttcagac ggcatttgcg ggattagaac
    27781 gtcgtgccga gttggaattg gaagcgttgg atttcgtctt ccggtttttt cttcagcggg
    27841 taggcgtagc tgaatttcat cgggcctaaa ggcgagagcc aggtaaccgc gccgccggcg
    27901 gaatagcgca attcgttggt aaaggtggat ttatgggtat tgccggcgcc gtaaatgttt
    27961 tgaaccctgc cgccggtcgc ggaactgctg ttgtcgtcgt aggttttgcc gtcccacacg
    28021 ctgcctgcgt cggcaaacag gctcaggcgg acggtgcgcg cgtctttcgc gccgggcatc
    28081 gggaagagca gctcggcgga gacgttggct tttttgttgc cgccgtagct gattttttcg
    28141 ccgtattcgt catagacttt cggaccaagt gtgccgcttt cgtaacctcg taccgaaccc
    28201 aggccgccgc cgtagaagtt ttcaaagaag gggatttctt tggttttgcc gtagccgttg
    28261 gcatagccga tttcgccgcc aagcatcagt gtgaaggttt tgctcagggg gaagaaccag
    28321 gtttggttgt gggtggcgga gtagtattgc agtttgctgc cgggcagggc gatttcggcg
    28381 ttcacgcccg tcaggtagcc gcgcgtcggc cataacgcgc tgtcggtttt gttgcgcccc
    28441 cagccgacgg tacctttgta cagccagcct ttgaagctgc cgtctgtgcc gtcggttttg
    28501 ccgtattgtt tgataaagtc ggcatagtgt ttgggcgctt tgttgtaggt gttgacggtc
    28561 aggtgttctg ccaccaaacc gaaattcacg cggtcgtatt cggtaacagg cacgctcatg
    28621 cggatgcctg cgcctgccgt ggtggtttta tattgtttga tgctggtcga tgctttgcgc
    28681 gggtcgaagg cttttccgta aacatcgtag cctaggctga ccccgtctgc cgtgaagtac
    28741 gggtcggtaa acgacagcga gccgttgagc gtggttttgc ttcgtgaggc gcgcagggcg
    28801 gccgacttgc ccgtaccgaa caggttgtct tgggaaacgc ctgcggacat gaccaggccg
    28861 gtatcctgta cccagcccgc gctcaagtcg agcgagccgg tggaacgttc ggtcaggctc
    28921 atgttcaaat cgactttgtc gggtgtgccg gcaagcggga cggcatcaaa ctgtacgttg
    28981 tcgaagtagc ccaaaagctc gacgcgctct ttggagcgtt gcagcttgga ggtgtcgtaa
    29041 ggcgcggatt ccatttggcg caattcgcgg cgcacgactt cgtcgcgggt tttgttgttg
    29101 ccggtgatgt ggatttcgtt gacgtagatt ttccggcccg gttcgatgtg caggacgaaa
    29161 tcgacggttt tggtttcggc gtttggcagc ggctgtacgc tgatttcgct gtatgcgtag
    29221 cctgccgagc ccatgcggtt ctgaatctca cccaaaacgg cggtcatctg ctggcgttcg
    29281 taccatttgc cgggcttcat ggtcagcagt ttttccagtt cggctttggg gacttcgttg
    29341 gtgtcgcctt cgatggagac tttgccccaa cggaaacgtc cgccttcgtg gacggtgatt
    29401 ttgatggtct gcttggtttt gtcttcgttg gtttggatgt cggtatcgag gatgcggaaa
    29461 tcgaagtagc cgttgttctg gtagaagtcg gttacttttt ccatgtcttg ggcaaatttc
    29521 tgctcgttga attggttgct tcgtgtcagc catgtccaaa tgccgccttc ggtcagcgac
    29581 atctgccgca tcagtttgcg gtcggaatag acttggttgc cttcaaattc gatgtcggtg
    29641 attttggcgg atttgccctc gtcaatcgtg atgtcgatgt cgacgcggtt gcgggcgagt
    29701 ttggttactt tgggcgtgat ttggatattg agtttgccgc gcccgaggta ttcttctttc
    29761 aggccggcga ctgcctgatt gagtgtcgcc tgattaaagt attgcgactg cgccagcccg
    29821 aacgattcga ggtttttctt aatggcgtcg ttttgcagca tttttgcgcc ggtgatgttg
    29881 agcgagccga tggtggggcg ttcgataacg gtcagcagga gctgcccgtc cgcagtttcg
    29941 acgcgtacgt cgtcaaagaa accggtggcg tacaggcttt tgatgatggc actgccgtgt
    30001 gtgtcgttgt aggtgtcgcc gactttgacg ggcaggtagt tgaatacggt actcggctcg
    30061 gtacgctgca agccttcgac gcggatgtct tggatggtga agtcggcaag tgccaaaggc
    30121 gatatgccca agaccatcag tgcggaagca atctgtttca gtttcattgt cagttccttg
    30181 tggtgcggaa tgcggtttca gacggcattc cgaaacgtaa aatctaaccg agcagccggg
    30241 taacgtcgtt gaagaaggcg accgccatca tcagcatcat gagggcaagc ccgaagcgca
    30301 aaccgatgtt ttggacgcgt tcgcccaaag gtttgccgcg tatccattcg gcagtataaa
    30361 acacgaggtg gccgccgtcc aaaacgggga cgggcagcag gttcagcacg ccgaggctga
    30421 tgctgaccag tgccaaaaat tccaaataac tttgcaagcc gagttcggcg gactgtccgg
    30481 caatgtcggc aatggtcagc ggcccggaaa tatggctgac ggaggcgttg ccgctgatta
    30541 gtttgccgaa aaatttgagg gttgtccacg agtgggaaac ggttttttcc cagcccatgc
    30601 cgaatgcgcg gataacagac ggacggtagc tgcggcggat ttgcgcgtcc cacgccctgt
    30661 ccggctgcgg acggaggccg acgcgcccga tcagggtgtg gtcgggctgt tcgacagtat
    30721 cggggcggat gtcggcggta tgggtttgtc cggcgcgttc gtaggtcagg gtgatttttt
    30781 tgccggggct ttggcgggtc aggtttgccc attcctgcca tgaggcgatg ggtttgccgt
    30841 cggcggcagt cagcctgtcg cccggtttca ggcctgcttt ttcggcgggg ctgccttttt
    30901 ccacgccgcc ggcaacggtt gtgattttaa agggcatcag tccgatgtag ccttggtttt
    30961 ttgcgatttt accggcttcc ggcgtgcctg cggcatcgat ggtgcggacg gtttgcgcgc
    31021 ccgatgccgt ctgaacgccg acggcgactt tgccggcttc gaggttgagg acgatttcgg
    31081 tttgcgcgct gccccaatct gcaacgggtg tgccgttgac ggattgtatt ttgtcgccgc
    31141 tttggaagcc ggcgcgggcg gcaatggtgt cgggttcgac tgtgccgaca taggggcgca
    31201 gttcggttac gccgaaggaa aagctcagtc cgtacagcaa aaccgccagt gcgagattgg
    31261 tcagcgggcc ggcggcgacg atggcgatgc gtttggcggg gtgttgtttg tcaaaagcgt
    31321 agggtaaatc ggcttctgat acttcgcctt cgcgcgtatc gaccattttg acgtaaccgc
    31381 ccaacggaat cggggcgagg caccattcgg tgtcgccgcg ctttcgggtg aaaaacggtt
    31441 tgccgaagcc gacggaaaag cgtacgactt tgacgccgca caatctggca acgatgtagt
    31501 gtccgaactc gtgcaggctg accaaaatca ggatggcgaa gataaaagct agaagggtgt
    31561 gcaaatggtt ttcctttgat aacggtgttc agacggcatc agcgcagtgt gccgataaat
    31621 gcccgcgctt gtgcgcgtgt ccgggcatct tgcgccaaga gcccccctat gtcgcctatg
    31681 ccgtctgaaa agtcttgtga aagacaatgg gcgacggttt tggcaatgtc ggtaaactta
    31741 atctgtccgt ccaaaaaggc ggcgacggcg gcttcgttgg cggcgttcaa tacgcagggc
    31801 gcggctccgc ctgcgttcat ggcttcatag gcgagcttca ggcaggggaa gcggtcaaag
    31861 tcgggctttt ggaaggtcag cgcggacaat gcgtcgaaat ccaggtcgcc gacacccgaa
    31921 tcgatgcgct cgggcaaacc caaacaataa gcaatgggcg tacgcatatc gggattgccc
    31981 agttgcgcca acacggagcc gtcgcggtag cgcaccatgc tgtgtatcac ggattgcgga
    32041 tggatgacga cttcgagctt gtcaggcgga cagttgaaca gccaatgcgc ttcaatcagc
    32101 tccaaacctt tgttcatcat ggtggcggaa tcgacggaga ttttgcgccc catgctccaa
    32161 ttggggtgtt tgaccgcttg ggcgggcgta atgctgtcga acgtgtttaa atcggcggtc
    32221 agaaacgggc cgccggaagc ggtcaggata atcgaagcga tgccgtgttc gttcagacgg
    32281 ccggcgtaat cgcgcggcaa aacttggaaa acggcgttgt gttcgctgtc gacgggcagc
    32341 actgccgcgc cgtttgcacg ggcggtttcc ataaacaacg cgccggaaac caccagcgtc
    32401 tctttgttcg ccagataaat ggttttgcct ttttgcgccg ctgcgagcgc ggaaggcagc
    32461 cccactgccc cgacgatggc gcacatgaca ccgctgactt cgtcggcaga cgcaacgtca
    32521 accaatgcct gcgcgccgtg taaaacctgc gtcgccgtgc cgtcgcgttt caacagggct
    32581 tcaagccggg cggcgtgttc cgcatcggca acgacggcat attcggggcg gaacgtttga
    32641 cattgagccg ccaatttctc gacctgctta tgccctgcca gcgcgaatac gcggaatttt
    32701 tcggggtggc gggagacaac gtccagcgtg ctttcgccta tgctgccggt actgcctaat
    32761 atggtcagga cttgtggtgt cataatgagg ataactttat accggatgcc gtctgaagcg
    32821 ttttcagacg gcatagaatc aatttaaaac cgacatcatc gccgcataga cgctgataac
    32881 ggcaatcagg ctgtcggtac ggtcgaacac gccgccgtgt ccggacagca ggttgctgct
    32941 gtctttaatg cccgccgcgc gcttgagcca gctttccaac aggtcgccgc atacgctgac
    33001 aacggtcagc accaaaccga ttaacacggt atcgaaccag cctgtatcga atgccagcca
    33061 gccggcactt cgtacggcgg tcatgtacac tgccacgcaa accgcgccgc cgattgcacc
    33121 ttcccagctt ttgccggggc tgattgccgg tgcgattttg tgtttgccga acgccttgcc
    33181 gctgaaatac gcgcaaatat cggcaaccca caccaaaccc atcacggcga gcagcggcag
    33241 ggcatcatcg ggatgcgggc gcagtgatac gagcgcgaac caaaacggca tgaccagaag
    33301 ccagccgacg gcataaacct gccaaccgcc gttgagcctc catttgaatc tcaaccataa
    33361 aggcataacg gcgagccaaa atgccaaaac aacataccaa accaaattag gcagcatcca
    33421 gccgcccgca taggcaacca cgccgaaaac caaggttgcg gcgaggtaat ggttggtttt
    33481 aattttgcac aaaccgccca tacgggcata ttcccacaag gcaatcaggg caatcagtcc
    33541 gcaaaatgca gcccacaacc attgcggcgc gtaaaacagc atgcccagca tcagcggcag
    33601 cagccacatg gcggttatta cccgttgttt cagcatattc agttcctttg ctgttcgata
    33661 ggcagttgct cggaggtgcg tccgaaccgc cgttcgcgtt tttggaacga agcgacggca
    33721 tcgtccaaag ccttgccgtc aaaatcgggc cacaaaatat cggtgaaata cagttctgca
    33781 tatgccatct gccagagcag gaaattgctg atgcgcgttt cgccgccggt gcggatgaac
    33841 aaatccggtt ccggtgcatc gcccagcatc aagtgtttcg ccagcgtgtc ttctgtaatc
    33901 tcggatacgc cttcggcaat cagtttgttt gccgcctgca aaatatccca gcggccgccg
    33961 taatcggcgg caatgctcag ggtcaggccg gtattgtttg ccgtcaacgc ctccgcttct
    34021 tcgatgcctt gcagaatctg ccggttgaag cgttcgcggc tgcccagtat tttcaggcgc
    34081 atattgtttt cgtgcagacg gcgtacctgt ttttgcaaag cctgtaaaaa cagccccatc
    34141 aggaacgaaa cttcgtcttc ggggcggcgc cagttttcgg ttgaaaaggc aaacacggtc
    34201 agatattgca cacccagttt ggcgcaatgc ttcaccatat tttccaatgc gtccaaaccg
    34261 cgtttgtgtc ccattatgcg cgggaggaaa cgttttttcg cccaacggcc gttgccgtcc
    34321 ataatcacgg cgatatgctt gggaatggcg gtgtgttcca aaacggcctg cgtgctgctt
    34381 ttcatgtctg cctttcgcgg ttcggcattc aaatgccgtc tgaacgccga accgtgcagg
    34441 ttaaattgcc atcaaatctt cttctttggc agtcaggagt ttgtcggctt cggtaatgta
    34501 tttgtcggtc agtttttgaa ccgcttcttc gccgcgacgt gcctcgtctt cggaaatttc
    34561 tttgtctttg aggagttttt tgatgtggtc gttggcatcg cggcgcacgt tgcggataga
    34621 gacgcggcct tcttccgctt cgccgcgtac gactttaatc aggtctttgc ggcgttcctc
    34681 ggtcagcatg ggcatcggca cgcggatcag gtcgccgaca gctgccgggt tcagccccaa
    34741 gtttgaatcg cggatggctt tctcgacttt ggccgccata ttgccctcaa acggtttcac
    34801 gccgatggtg cgcgcgtcca gaagcgttac gttggcaact tggctgacgg ggaccatgct
    34861 gccccagtat tcgacttcca cttggtcgag caggccggta tgcgcgcggc cggtacgcac
    34921 tttcgccaga ttttctttca gtacttcgac cgaacgctgc attttgcctt cggctgtttt
    34981 ttgaatatcg ttgatcatat tgttctttcg gtgggataag gtgggcggga gaccgtctga
    35041 acgcgtttca aacctttcag acggcatgaa aactgttaac tgcgaatagt accgttattc
    35101 gggcatgacg acaaggcagg cggattgggg atgccgtctg aagcggcagg cgtttcagac
    35161 ggcatcgtgt ccgaccgtca gccgtgttcc cgtgtttcaa gcaggctttg gcgcaggtgt
    35221 tggcgttcgt gggcatccag ccatttgcgg cgggtgcgtt gcagcaggat gacgagggcg
    35281 gaaatttcct gacgcatatt ggtgctgagc cagaggaagc cctgccattg gtagtggagg
    35341 tgttcggcga gggcttccag ttcggggttg atggcggtgt cgatgcggat gcggcgggcg
    35401 tgtctgccgt tgataagggc gacggtttgt tgcaggtcgg tttggagcag tgtgaagtgg
    35461 cggtcaagca gccggatttc gctgccgttg agtttgggag attgcagctt ggcggcagtg
    35521 gtcaggagca gctcggtggt gttgacaatt ttacggtggg cgtgctgcat ggcttccatc
    35581 atggcggggc tgatgcggct ttcgcccgat gtggcggcga ggtggctgcg gcttttgacc
    35641 atgcgtgcgt tgatttggcg cattttaacc atattctgct ccaaacgttc gcgcgtcata
    35701 cgcctgccgt tgctgatttc ggcaatcatt ttgctgcagt cggtcaggtt gtcggcaagc
    35761 atgaaacgcc acatcagtgt ggatttcagc ggcagcagtt tggcggcggc gatggcgatg
    35821 gccgcgccga tgaggacgtt catcgcgcgc atcaggccgc tgtcgaacca ttcgctgccg
    35881 ttgtcgccga tgagcatgca catcgtcagc cccgccagca tagggacgta gccgtttttg
    35941 ccgaccgccg cccagccggc cagtgcgctt gccgtgccga cggtgaggta gaagaggagg
    36001 ttgccgtgga aataatgctg gttcagccat aaaacgccca aacccgcgcc cagcccgatg
    36061 accgtgccga gcatacgttc caccgccttg gagtaaatcg ccccttgaaa ctggagcatg
    36121 ccgaggacga cgaagacggt catgcctatc cattcgccgt gttggaggtg gagcagccgg
    36181 gcgagtgcgg tggcgaacag gacggccccg ccgagccgca cggcgtgtat gaggcggcgg
    36241 tggcggtagc gttcgtagga gttgagccag cggctgacga ggcggttgcg ttgcgaggtg
    36301 ttcatatcgg ttgccgtctg aagcggaaat gtgaaaaagc acaggcttcc cgaggaaggg
    36361 agggtctgtg cttggtattg gtgccggaga agggaatcga acccccgacc ttcgcgttac
    36421 gaatgcgctg ctctaccgac tgagctacac cggcgttttt tcgttatgat atatatgaac
    36481 ggttgtttgt gcaacttttc tggcgggcgg caaggcagtg cgcggtataa tgcggtctgc
    36541 ttcggaagag ggggacggcg atgtttgtga acgagaaaga tccttatgcg gctctgtttg
    36601 cggggctggt gtttttgacg ttgccgtttg cgttggcggt gcatgatgcc tttgcgcttg
    36661 cgttcggacg ggcagggctg ctggtgtcgg tgtcggccgg cgtggcggtt gggacggtac
    36721 tgtttggttt gtgttcggtg tgtttgcgtt tttgaacctg gtcgtgtcgg caggcctgac
    36781 gaagctggcg tacaaaaaga tgatgcggcg gcattcccgt tacgcgctgt ttctgtcggg
    36841 cgtggcggct tgcgcggcgg cagcggtggc ttggattttc gagctgctgc ttggcagtgg
    36901 ggctttgggc gggctgcgtg gggaggcggt gttggaatat gcgtttgccg tgtggctggt
    36961 ggcgatgctg acgctgccca aacgcctgac gcgcgcgccg gtgcagccgg tggtgtttca
    37021 caggaaaaaa taggttggaa cgggaaatgc cgtctgaacc ccgacacgcg gtttcagacg
    37081 gcatgttttt ccgttaacat tacgcctgaa tatggacagg aagcagatat ggaacgcaaa
    37141 gaacgcctgc aatcaggcat tgccgcgatg gggctggata tttcggaaac ggcgcaggac
    37201 aggcttttgg cctatgtgga tttgttgaaa aagtggaaca aaacctacaa tctgaccgcc
    37261 ctgcgcgacg aggaaaaaat gattgtccat catcttttgg acagcctgac gctgctgccc
    37321 catatcgagg gtgtgcaaac gatgctggat gtcggttcgg gcggcggtca gcccggcatt
    37381 ccggcggcgg tgtgccgtcc ggatgtgcag ataacccttt tggatgcgaa tacgaagaaa
    37441 acggcttttt tacagcaggc ggttatcgag ttggggttgg acaatgtgcg cgtggtatcc
    37501 ggacgcgtgg aggcggtttc ggacgtgcgt gccgacgtgg ttaccagccg tgcgtttgca
    37561 gaactggcgg attttgtgtc gtggacggtg catctgttga aagacggcgg ctactgggcg
    37621 gcgatgaagg gcgtgtatcc gcagggggaa atcgaccacc tgccgcagga tgtgtgcgtc
    37681 gaaaaagtcc aaaggcttga cgtgccgggc ttggatgcgg aacgccatat cgtcatactc
    37741 cgcaagcgtt gagcgcgctt cagacggcat gaataccttt tttgtgcgga taaaggtaaa
    37801 attccgcact gtttttcttt tttcaacatc agacgggaca cgggcgggac atgagtgcga
    37861 acatccttgc catcgccaat cagaagggcg gtgtgggcaa aacgacgacg acggtaaatt
    37921 tggcggcttc gctggcatcg cgcggcaaac gcgtgctggt ggtcgatttg gatccgcagg
    37981 gcaatgcgac gacgggcagc ggcatcgaca aggcgagttt gcagtccggc gtttatcagg
    38041 tcttattggg cgatgcggac gtgcagtcgg cggcggtacg cagcaaagag ggcggatacg
    38101 ctgtgttggg tgcgaaccgc gcgctggccg gcgcggaaat cgagttggta caggaaatcg
    38161 cccgggaagt gcgtttgaaa aacgcgctca aggcagtggc ggaagattac gactttatcc
    38221 tgatcgactg cccgccttcg ctgacgctgt tgacgcttaa cgggctggtg gcagccggcg
    38281 gcgtgattgt gccgatgttg tgcgaatatt acgcgctgga agggatttcc gatttgattg
    38341 cgaccgtgcg caaaattcgt caggcggtca atcccgattt ggacatcacg ggcatcgtgc
    38401 gcacgatgta cgacagccgc agcaggctgg ttgccgaagt cagcgaacag ttgcgcagcc
    38461 atttcgggga tttgctgttt gaaaccgtca tcccgcgcaa tatccgcctt gcggaagcgc
    38521 cgagccacgg tatgccggtg atggcttatg atgcgcaggc aaagggtacc aaggcgtatc
    38581 ttgccttggc ggacgagctg gcggcgaggg tgtcggggaa ataggtcaat ccaaatcggg
    38641 ctgcccgtgc ctttatgctg tttggccggg tgcgttaata tggcggatta aaataaaaat
    38701 acttatatcg t
//
LOCUS       DAWHXZ010000002        28877 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000002 DAWHXZ010000000
VERSION     DAWHXZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28877
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.002"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            184..540
                     /locus_tag="WIE35_000055"
     CDS             184..540
                     /locus_tag="WIE35_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ4153049.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGVYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            531..1013
                     /locus_tag="WIE35_000056"
     CDS             531..1013
                     /locus_tag="WIE35_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ4153050.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            1026..1619
                     /locus_tag="WIE35_000057"
     CDS             1026..1619
                     /locus_tag="WIE35_000057"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ4153051.1"
                     /translation="MASIQNLYETVIGVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEDLHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            1609..2865
                     /gene="nuoD"
                     /locus_tag="WIE35_000058"
     CDS             1609..2865
                     /gene="nuoD"
                     /locus_tag="WIE35_000058"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ4153052.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            2865..3338
                     /gene="nuoE"
                     /locus_tag="WIE35_000059"
     CDS             2865..3338
                     /gene="nuoE"
                     /locus_tag="WIE35_000059"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ4153053.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            3728..5028
                     /gene="nuoF"
                     /locus_tag="WIE35_000060"
                     /pseudo
     CDS             3728..5028
                     /gene="nuoF"
                     /locus_tag="WIE35_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
     gene            5028..5465
                     /locus_tag="WIE35_000061"
     CDS             5028..5465
                     /locus_tag="WIE35_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153054.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICWKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            5755..8016
                     /locus_tag="WIE35_000062"
     CDS             5755..8016
                     /locus_tag="WIE35_000062"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ4153055.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGSLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFSYEGLYHESRLKK
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELLMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     TEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLSSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDAIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            8019..9095
                     /gene="nuoH"
                     /locus_tag="WIE35_000063"
     CDS             8019..9095
                     /gene="nuoH"
                     /locus_tag="WIE35_000063"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ4153056.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            9176..9655
                     /gene="nuoI"
                     /locus_tag="WIE35_000064"
     CDS             9176..9655
                     /gene="nuoI"
                     /locus_tag="WIE35_000064"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ4153057.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            9667..10338
                     /locus_tag="WIE35_000065"
     CDS             9667..10338
                     /locus_tag="WIE35_000065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ4153058.1"
                     /translation="MTFSAILFYILATIVLYGALRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTINPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            10335..10640
                     /gene="nuoK"
                     /locus_tag="WIE35_000066"
     CDS             10335..10640
                     /gene="nuoK"
                     /locus_tag="WIE35_000066"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ4153059.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLNKLKG"
     gene            10935..11807
                     /locus_tag="WIE35_000067"
     CDS             10935..11807
                     /locus_tag="WIE35_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEZ4153060.1"
                     /translation="MSFSIALPLVYLFIGILIGKTPFRKIKGTFSVILSRWIIPFVIV
                     YNITTYRDGMQYVMLWMIISMGLIILIGKRVDKDPVWNLCLCYLNIGWLGLPIVATLF
                     GDEAAQVLISAYVGSSLFGNSVGVGLLLNNSNFISGLKKTLKSPPVVALVIGMFGIPL
                     GHFVADYFGEVYFFSKIAMGILGMVILGIWLAEIKLNLNDLKKSIKPFLIKNILFIGI
                     VFLFIQIAAYFDLKLILENQKTLYLIPLLPPAANIIVLETAYLKTGRSASMIAYGTIF
                     SLIAISIYILLVQI"
     gene            11808..13832
                     /gene="nuoL"
                     /locus_tag="WIE35_000068"
     CDS             11808..13832
                     /gene="nuoL"
                     /locus_tag="WIE35_000068"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ4153061.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYLAI
                     EPMLYGDFFKDVIFVNTDAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            14016..15179
                     /locus_tag="WIE35_000069"
     CDS             14016..15179
                     /locus_tag="WIE35_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ4153062.1"
                     /translation="MTQLFLHSDTPNEKELISIYEKAVSEAVELYIFSAYLTHWDVKN
                     KLNSKLQEFKFIFGQDFGLSKKEAIRKVLKWLPSHLKFTLMVAQGIQGFHPKAVFWKN
                     DKNEYYALIGSSNLTHAAFNSNYEANILTKISEQDFIKVKSWADEIAMKSIPVSEDWL
                     EEYQEAEINYKKSTVSQSLMDKLFMEMPNYNQELIAARRKQMRNHQTVCNQLKSLIKQ
                     CAAGKIDNDEFYVEFNKLWSWKSENKGEGVGNRFQDQTWKRTGKSSDFRKLCIAIQSV
                     FDAPLAERDNVVAKQIDWLKECGVSTRGSVFSEMLCQEYPDRYPVLNAPIKKFLEKNK
                     FKSAKGASEGSRYIDLSMKLRALLAMQSEIKNLAELDVLVQAEYRDSTDIDWE"
     gene            15289..16785
                     /locus_tag="WIE35_000070"
     CDS             15289..16785
                     /locus_tag="WIE35_000070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ4153063.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLSTGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGEIRNPHVADM
                     KDINCREFAVLAVLAAAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            16795..18240
                     /gene="nuoN"
                     /locus_tag="WIE35_000071"
     CDS             16795..18240
                     /gene="nuoN"
                     /locus_tag="WIE35_000071"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ4153064.1"
                     /translation="MNWSDLNLMPAMPEIVLLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYARPYNQVRG
                     IFKGEFYTLSLFALLGMGVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVG
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKVAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARLAAGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            18352..18651
                     /locus_tag="WIE35_000072"
     CDS             18352..18651
                     /locus_tag="WIE35_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ4153065.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHNQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(18760..19656)
                     /locus_tag="WIE35_000073"
     CDS             complement(18760..19656)
                     /locus_tag="WIE35_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ4153066.1"
                     /translation="MNPTNDLKAWQLRAQAQTELLLERFLPSEGEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIQYDEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(19640..19864)
                     /locus_tag="WIE35_000074"
     CDS             complement(19640..19864)
                     /locus_tag="WIE35_000074"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ4153067.1"
                     /translation="MKKNAPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGLKELNLESDE"
     gene            complement(20111..21034)
                     /gene="rsgA"
                     /locus_tag="WIE35_000075"
     CDS             complement(20111..21034)
                     /gene="rsgA"
                     /locus_tag="WIE35_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ4153068.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIEAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            complement(21039..22895)
                     /locus_tag="WIE35_000076"
     CDS             complement(21039..22895)
                     /locus_tag="WIE35_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ4153069.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAY
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVSQGEIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(22921..23058)
                     /locus_tag="WIE35_000077"
     CDS             complement(22921..23058)
                     /locus_tag="WIE35_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153070.1"
                     /translation="MPSEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(23058..23642)
                     /gene="ruvA"
                     /locus_tag="WIE35_000078"
     CDS             complement(23058..23642)
                     /gene="ruvA"
                     /locus_tag="WIE35_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ4153071.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            complement(23724..24050)
                     /locus_tag="WIE35_000079"
     CDS             complement(23724..24050)
                     /locus_tag="WIE35_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ4153072.1"
                     /translation="MNIKHLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(24112..24831)
                     /locus_tag="WIE35_000080"
     CDS             complement(24112..24831)
                     /locus_tag="WIE35_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ4153073.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSALV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNLASSASNPNLSVEKRHYEYVVKMGPFSSQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            complement(24828..24950)
                     /locus_tag="WIE35_000081"
     CDS             complement(24828..24950)
                     /locus_tag="WIE35_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ4153074.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            complement(24961..25425)
                     /gene="trmL"
                     /locus_tag="WIE35_000082"
     CDS             complement(24961..25425)
                     /gene="trmL"
                     /locus_tag="WIE35_000082"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ4153075.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(25490..25987)
                     /locus_tag="WIE35_000083"
     CDS             complement(25490..25987)
                     /locus_tag="WIE35_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ4153076.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     GERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKSGANRICCWTLAR
                     TPMKK"
     gene            26082..26957
                     /gene="bioH"
                     /locus_tag="WIE35_000084"
     CDS             26082..26957
                     /gene="bioH"
                     /locus_tag="WIE35_000084"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ4153077.1"
                     /translation="MRYAAKSSRQPAQMPSETPDEPWHNTQRLIVGASAMRRQQERKS
                     MPDAVKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGDAPFAQPFDIEAAAD
                     AVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARLTADEDYPEGLAAP
                     ALGKMVGAFRTDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARCGTPSALQEALDAA
                     ERADARHLLDKIDVPVLLVFGSKDAITPPRMGEYLHRRLKGSRLVVMEKAAHAPFLSH
                     AEAFAALYRDFVEGV"
     gene            26959..27747
                     /locus_tag="WIE35_000085"
     CDS             26959..27747
                     /locus_tag="WIE35_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ4153078.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKYILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFTGKGVVQHCQSPIAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(27828..27929)
                     /locus_tag="WIE35_000086"
                     /pseudo
     CDS             complement(27828..27929)
                     /locus_tag="WIE35_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            27918..28154
                     /locus_tag="WIE35_000087"
     CDS             27918..28154
                     /locus_tag="WIE35_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153079.1"
                     /translation="MFVNPLYADAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTHATL
                     LALDWQGNKPLGAEELADLKPLYKDLKNNIGNIV"
     gene            28485..>28877
                     /locus_tag="WIE35_000088"
     CDS             28485..>28877
                     /locus_tag="WIE35_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HEZ4153080.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYDSGLTKTSTAL"
BASE COUNT         6471 a         7096 c         7934 g         7376 t
ORIGIN      
        1 cacgaattac ggtgtaaaaa tttatatgac cttataaaat caaataagaa tcgttatcat
       61 aacatgattg tatttattgg gtttttttgg gcgttttgcc gatatttacc ttttaatggt
      121 ttttgaaatt cgctaaaata cgaaattatt gtagaaattt tgttaacgga tttgggtgta
      181 accatgttgt ccgcttactt tcccgtcttt gtctttatcc ttgtcggtct tgcagccggc
      241 gtactgttta tcctgctcgg cacaattttg ggcccgaaac gtcactatgc cgaaaaagac
      301 gcgccttacg aatgcggttt tgaagccttt gaaaacgcaa ggatgaagtt cgacgtgcgc
      361 tattacctcg tcgccatcct cttcatcctg tttgatttgg aggtcgcgtt tatgctgccg
      421 tgggctgtcg tgttcaaaga tttgggcgtg tacggcttct ggtctatgct ggtgtttatc
      481 gtcgttctga cggtaggctt tgtttacgaa tggaaaaaag gtgcgctgga atgggaatag
      541 aaggcgtttt gaaaaaaggt ttcatcacca ccagcgcgga tacggtgctg aactatatgc
      601 gtaccggttc attgtggccg gttactttcg gcttggcctg ctgcgccgtg gaaatgatgc
      661 atgcaggtat ggcgcgttat gaccttgacc gtttcggcat cattttccgt ccgtccccac
      721 gtcaggccga cctgatgatt gtggcaggta cgctgaccaa caaaatggcg cccgccctgc
      781 gccgcgtata cgaccagctc gccgagccgc gttgggtatt gtctatgggc tcgtgtgcca
      841 acggcggcgg ttattatcac tattcttatt ccgttgtgcg cggtgccgac cgcgtcgtgc
      901 cggtagacgt ttatgtgccg ggttgtccgc cgactgcgga agccctgatt tacggcctga
      961 ttcagctcca acaaaaaatc aagcgcactt ccaccatcgc gcgtgacgag taaggagagg
     1021 acgatatggc aagcattcaa aatttatacg agaccgtcat cggtgtgctg ggcaatcagg
     1081 caggcaaagt catttcagct ttgggcgaga ttaccgtcga gtgtctgccc gaacactata
     1141 tctcagtcat gaccgcattg cgcgaccatg aagatttgca tttcgagctt ctggtcgatt
     1201 tgtgcggtgt cgattacagc acttacaaaa acgaagtatg gcagggcaaa cgctttgccg
     1261 tcgtcagcca gctgctttcc gttaaaaaca atcaacgcat ccgcgtacgc gtctgggttt
     1321 cagacgacga cttccccgta gtcgaatccg tagtcgatat ttacaacagc gcggattggt
     1381 acgaacgcga agccttcgat atgtacggca tcatgttcaa caaccacccg gacttgcgcc
     1441 gcatcctgac cgattacggc tttgtcggac atccgttccg caaagacttc ccgatttccg
     1501 gctatgtgga aatgcgttac gacgaagagc aaaaacgcgt gatttaccaa cctgttacca
     1561 ttgagccgcg cgagatcacg ccgcgtatcg tccgtgagga gaactacggt ggccaataaa
     1621 ttaagaaact acaccatcaa cttcggcccg caacaccctg cggcgcacgg cgtattgcgt
     1681 atgattttgg agttggaggg cgaaaccatc gtccgtgccg atccgcatat cggcctcctg
     1741 caccgcggta ctgaaaaact ggctgaaacc aaaacctatc tgcaagccct gccctatatg
     1801 gaccgcttgg actacgtttc catgatggtc aatgagcagg cgtattgttt ggcagtagaa
     1861 aaacttgccg gtatcgatgt acctatccgc gcccagtata tccgtgtgat gtttgccgaa
     1921 gtaacgcgca tcctcaatca cttgatgggc atcggttcgc atgccttcga catcggcgcg
     1981 atgaccgcca tcctctacgc cttccgcgac cgcgaagagc tgatggactt gtacgaagcc
     2041 gtttccggcg cgcgtatgca cgcagcctac ttccgtcccg gcggcgttta ccgcgacctg
     2101 cccgacttca tgcctaaata cgagagcagc aaattccgca atgccaaagt attgaagcag
     2161 ctcaacgaat cccgcgaagg caccatgctc gactttatcg atgccttctg cgaacgcttc
     2221 cccaaaaata tcgacacact cgaaaccctc ctgaccgaca accgcatctg gaaacagcgt
     2281 accgtcggca tcggcgtcgt ctcccccgaa cgcgccatgc aaaaaggctt taccggcgtg
     2341 atgttgcgcg gttcgggcgt ggaatgggac gtgcgtaaga cacagcctta cgaagtgtac
     2401 gacaaaatgg atttcgacat ccccgtcggc gtcaacggcg actgttacga ccgttacctc
     2461 tgccgtatgg aagaaatgcg tcaatccgta cgcatcatca aacaatgttc cgagtggttg
     2521 cgtgtcaatc cgggtccggt cattaccaca aaccacaaat ttgctccgcc caaacgtacc
     2581 gaaatgaaaa caggtatgga agacctgatt caccatttca aactctttac cgagggtatg
     2641 cacgttcccg agggtgagac ctacaccgct gtcgaacatc cgaaaggcga gttcggcgtt
     2701 tacatcattt cagacggcgc aaacaaaccc taccgcctga aaatccgcgc acccggcttc
     2761 gcccatctgc aaggcatgga cgaaatggca aaaggccaca tgctcgccga cgtcgttgcc
     2821 atcatcggta cgcaggacat cgtattcggg gaggttgacc gataatgtta tccgcagagt
     2881 ccttaaaaca aatcgacaca gaactggcga aatatcccgc cgaccaacgc cgttccgcca
     2941 tcatgggtgc gttgcgtatc gcccaaaccg aaaaaggctg gctcgccccc gaaaccatcg
     3001 cttttgttgc cgactatatc ggcatcacgc ctgcacaagc ctacgaagtc gccactttct
     3061 acaatatgta cgaccttgag cctgtcggca aatacaaact gaccgtttgt accaacctgc
     3121 cctgcgccct gcgcggcggt atggctaccg gcgaatacct caaacaaaaa ctcggtatcg
     3181 gctacggcga aactacccct gacggcaagt ttacccttgt cgaaggcgaa tgcatgggcg
     3241 catgcggcga cgctcccgtt atgctggtca acaaccacag catgtgcagc tttatgaccg
     3301 aagaagcgat tgagaagaaa ctggcggagt tggagtaggt cgtctgaaac gacgatttaa
     3361 acgtaggtcg gatacttgta gccgacagag tgggtaaaaa ggcaaaatgt cggatttaag
     3421 aatccgccct actgaaatac cgaaatgccg tcattcccgc gcaggcggga atccaccggt
     3481 aagattcggt ttctgaattt aataagacat tgcttaccat tgaggatgga ttcccgcctg
     3541 cgcgggaatg acgacagaca agcaagtggt cgagatccaa caaaaacgat taaaggtcgt
     3601 ctgaaaatat cgatttgata aactagattt tatttcagac gacgttacaa gccggtacac
     3661 aaagacatct taaggtcgtc tgaaacagcg gccgcaaccg atacgaaaac aaacaggcac
     3721 accaaaaatg gctatttacc aatcaggcgt gatttttgac caagtggata ccgccaatcc
     3781 cgattgctgg acattggacg aatacgtcaa acgtggcggt tataccgccc tgcgtaaaat
     3841 cctgtccgaa aacatctcgc aaaccgatgt gattgacgaa gtcaaaacct ccggcttgcg
     3901 cggtcgtggc ggtgcgggct tcccgaccgg tttgaaatgg agctttatgc cccgttcttt
     3961 ccccggcgaa aagtacgtcg tttgcaacac cgacgaaggc gagccgggta catttaaaga
     4021 ccgcgacatt attatgttca accccatgcc ctgattgaag gcatgattat cgccggttac
     4081 gcgatgggcg cgaaagccgg ttacaactat atccacggcg aaatttttga aggctaccaa
     4141 cgctttgagg ccgctttgga gcaggcgcgt gccgcaggct ttttgggtaa aaatattttg
     4201 ggttcggatt ttgaattcga actcttcgcc caccacggct acggtgcata catctgcggc
     4261 gaagaaaccg cattgcttga atcgctggaa ggcaaaaaag gccagccgcg ctttaaaccg
     4321 ccattccctg cttcgttcgg cctgtacggc aaaccgacta ccatcaacaa taccgaaact
     4381 ttctcctccg tttcattcat tatccgtgac ggcggacagg catttgccga taaaggtatt
     4441 ccgaatgcag gcggtaccaa attattctgt atttccggcc atgtcgagcg tccgggcaac
     4501 tatgaagtgc cattgggcac accgtttgcc gaagtcttga aaatggcagg cggtatgcgc
     4561 ggcggcaaaa aactcaaggc cgttattcct ggcggttcgt ccgcgcccgt attgcctgcc
     4621 gacatcatga tgcagacaaa tatggactac gactcgattt ccaaagcagg ctcgatgctg
     4681 ggttctggcg cgattatcgt catggacgaa gacgtgtgca tggtgaaagc ccttgagcgc
     4741 ctgagctact tctactacga cgagtcttgc ggccaatgta cgccttgccg agaaggcacc
     4801 ggctggcttt accgcatcgt ccaccgcatc gtagaaggca aaggccgcat ggaagatttg
     4861 gatttgctgg attctgtcgg caaccaaatg gcaggccgca ccatctgcgc ccttgccgat
     4921 gccgccgtct tccccgtccg aagctttacc aagcatttcc gtgatgagtt tgtgcattac
     4981 atcgaacacg gcggaccgat gaaagagcat aagtggggag ggtggtaatg gtggaagcta
     5041 aaatttttat tctatacggt gcagccaaca aaggtaagag tacgacactc aatacgcttt
     5101 ttaatcagat ttgttggaaa ttttctaaat ttctagtctt ttttgaaaga catggaaatg
     5161 gcttagattt tgttgcagta tttgatcatg aaggtcagag aattggtttt tattcatctg
     5221 gtgataatga atacgaggtt aggagaaatt tatacaaact ttattcgcat aattgtgatt
     5281 ttatttttgg cacgtcaagg acacggggtg gtagttgcga tgcagtagga tgttatgcag
     5341 agttattgca tggcgatgta aatataatta attggtgtga aaagtttgag cctacagatg
     5401 aagacaatga gcgtgctgtt aaagagttat ttaagtcatt taaaaatata ataaatgagt
     5461 tatagtttta gttggtttta tattggttaa aagcaaaatg ctaaaaattt aactttgccg
     5521 tcattcccgc gtaggcggga atccatagtg gaatttacag aacccgatat ttgaaaagca
     5581 gttgccgaaa ttcaaaaaat ggattcccgc ctgtgcggga atgacggcaa ggtggcagta
     5641 aacgttttaa acagtattga ttgtcaatga aactcaaaag gccgtctgaa acccattttt
     5701 cagacgacct ccataaaaga ttatttatca aatacccgta actaggaacg aaccatgtta
     5761 caaatcgaaa tcgacggcaa acaagtatct gtggagcagg gcgcgacggt gattgaagcc
     5821 gcgcacaagc tcggtactta tattccgcat ttctgttacc acaaaaaact ttccatcgcc
     5881 gccaactgcc gtatgtgtct ggtgaacgta gaaaaagccc caaaacccct gcctgcctgt
     5941 gccacgccgg ttacagacgg catgattgtg cgtacgcatt cggcaaaagc ccgagaggcg
     6001 caggaaggcg tgatggagtt cctgctcatc aaccatccgc ttgattgtcc gacctgcgac
     6061 caaggcgggg aatgccagtt gcaggatttg gcggtgggct acggaaaaac caccagccgc
     6121 tacaccgaag aaaaacgttc cgtcgtcggc aaagatatgg ggtccttggt ttccgccgag
     6181 gaaatgagcc gctgtatcca ctgcacccgc tgcgttcgtt tcactgaaga aatcgccggt
     6241 ttgcaggaaa ttgcgatggt gaatcgcggc gaacactccg aaatcatgcc ctttatcggc
     6301 aaaacggtgg aaaccgaatt gtcgggcaac gtcattgatt tgtgtcccgt cggcgcgctg
     6361 accagcaaac cgttccgctt caatgcgcgt acttgggaat tgaaccgccg caaatccgtt
     6421 tccgcccacg acgctttggg cagcaacctg attgtacaaa ccaaagacca taccgtccgc
     6481 cgcgtgttgc cgctggaaaa tgaagcgatt aacgaatgtt ggctgtctga ccgcgaccgt
     6541 ttttcctacg aaggcctgta tcacgaaagc cgtctgaaaa aaccgaaaat caaacagggc
     6601 ggcgagtgga tggacgtgga ttggaaaacc gcgttggaat atgtccgcag cgcgattgaa
     6661 tgtatcgcca aagacggcaa ccaaaaccaa gtcggcattt gggcgaaccc gatgaatacg
     6721 gttgaagagc tgtatctggc caaaaaactt gccgacggct tgggtgttaa aaactttgcc
     6781 acccgtttgc gccaacaaga caaacgtctt tcagacggcc ttaaaggtgc gcaatggttg
     6841 ggacaaagca ttgaatcgct ggcggacaac gatgccgtat tggtagtcgg tgcgaacctg
     6901 cgcaaagaac agccgctcct gaccgcccgc ctgcgccgcg ccgccaaaga ccgtatggcg
     6961 ttgagtgtat tggccggcag taaagaagag ttgttgatgc cgcttctctc tcaagaagcc
     7021 gcacatcccg acgagtgggc aggccgtctg aaaaacctgt ctgccaatgc ggaacacgcc
     7081 gttaccgcca gcctgaaaaa cgctgaaaaa gcagcggtga ttttgggcgc ggaagtgcaa
     7141 aaccatcctg attacgccgc catttatgcc gccgcgcaag agctggcgga tgcaaccggc
     7201 gcggtactgg gtattttgcc gcaagccgcc aacagcgtcg gtgcggacgt attgggcgtg
     7261 aactcaggtg agagcgttac cgaaatggca aacgcgccga aacaggcagt cttgctgctc
     7321 aacgtcgagc ctgaaattga tacggttgac ggtgcaaaag ccgtagccgc gttgaaacag
     7381 gcaaaaagcg tgatggcgtt tacaccgttt gtcagcgaaa cgctgctgga cgtgtgcgac
     7441 gtgttgttgc cgattgcgcc gtttaccgaa acctcaggca gcttcatcaa tatggaaggc
     7501 cgtctgcaat ctttccacgg cgtggtacaa ggcttcggcg attcgcgtcc gatgtggaaa
     7561 gtgttgcgcg tattgggcaa tctgtttgac ctgaaaggtt ttgaatacca cgataccgct
     7621 gcgattttga aagacgcgct ggatgcagaa agcctgtcgt ccaaactgga caaccgcagt
     7681 acatggacag gggagggcgt tcagacggca tcagaccgcc tcgtccgtgt cggcggcgtc
     7741 ggtatttatc acaccgatgc catcgtacgc cgttccgcac cgttgcaaga aaccagccat
     7801 gccgccgtgc ctgccgcgcg tgtaaatccg aacacattgg cacgcttggg cctgcaagac
     7861 ggacaaaccg ctgtcgccaa acaaaacggc gcaagcgtat cggttgctgt caaagccgat
     7921 gccggtttgc ctgaaaacgt ggtgcatctg ccgctgcata ccgaaaatgc cgcgctgggt
     7981 gcgttgatgg acactattga actggcggga gcttgatcat gcaagaatgg ttccaaaacc
     8041 tctttgccgc aacgctcggt ctgggcgatt tgggcatcac cgtaggcttg gtggtatccg
     8101 tcatcgtcaa aatcgtgatt atcctgattc cgctgattct gaccgttgcc tacctgacgt
     8161 atttcgaacg taaagtcatc ggcttcatgc agcttcgcgt cggtccgaac gtaaccggcc
     8221 cgtggggtct gattcagccg tttgccgacg tgttcaaact cttgtttaaa gaggtaaccc
     8281 gtccgaagct gtcaaacaaa gccctgttct acatcggccc gattatgtcg cttgctccgt
     8341 ctttcgcagc atgggcagtg attccgttca acgaagaatg ggtgctgacc aacatcaata
     8401 tcggcctttt gtacatcctg atgattacct cgctgtcggt ttacggcgtg atcatcgcgg
     8461 gctgggcttc caactccaaa tattcgttct tgggtgcaat gcgtgcttcc gcacaaagca
     8521 tttcctacga gattgccatg agtgccgcgc tggtgtgcgt tgtgatggta tcgggcagca
     8581 tgaacttctc cgacatcgtt gccgcacaag ccaaaggcat cgcgggcggt tccgtgttct
     8641 cgtggaactg gctgccgctc ttccccatct tcatcgttta tctgatttcc gccgttgccg
     8701 aaaccaaccg tgcgccgttt gacgtggcag agggtgagtc tgaaatcgtt gccggtcacc
     8761 acgtcgaata ttccggcttc gcgttcgcgc tgttcttcct tgccgaatac attttcatga
     8821 ttctgattgc cgcgctgaca tcgctgatgt tcctcggcgg ctggttgtcc ccgttcccgc
     8881 aaagttgggg ctttatcggc acaccttccg cattctggat gttcgtgaaa atggcggcgg
     8941 tgttgtactg gtacctgtgg atccgtgcaa ccttcccgcg ctaccgttac gaccaaatca
     9001 tgcgtttggg ctggaaagtg ctgattccga tcggcttcgc ctacatcgtg gttttgggcg
     9061 tgtggatgat ttcaccgctg aatttgtgga aataagtttc agacggcatc ttgaggccgt
     9121 ctgaacaaag cgattttgaa tacctaacga aatccctgtt ttgagggaac ataatatggc
     9181 taacttagta aaaacctttc tgcttggcga attggtaaaa ggtatgggcg taacgctcaa
     9241 aaactttttc gcccgcaaag acacgattta ttttcccgaa gagaaaacgc cgcaatccgt
     9301 gcgtttccgc ggcctgcacg cgcagcggcg gtatccgaac ggtgaggaac gctgcattgc
     9361 gtgtaagttg tgcgaggcgg tgtgtccggc aatggcgatc aatatcgaat cggaagaacg
     9421 cgaagacggc acccgccgca ccaagcgtta cgacatcgac ctgaccaagt gcatcttctg
     9481 cggcttctgc gaagaggcct gcccaaccga tgcgattgtg gaaacgcata tttttgaata
     9541 ccacggcgag aaaaaaggcg acttgcacat gaccaagccg atcctcttgg caatcggcga
     9601 caaatacgaa gccgaaatcg ccaaacgcaa agccgctgac gcgccgtacc gttaaggagc
     9661 agacgaatga ctttttccgc gattctgttc tatatccttg ccaccatcgt cttatacggc
     9721 gcgctgcgta ccgttaccgc taaaaaccct gtccacgccg ctttgcatct ggtgctgacc
     9781 ttctgcgtga gcgcgatgct ttggatgctg atgcaggctg agtttttggg cgtgacgctg
     9841 gtggtggttt acgtcggcgc cgtgatggtg ttgttcctgt tcgtcgtgat gatgttgaac
     9901 atcgacattg aagaaatgcg tgccggtttc tggcggcacg cgcctgttgc cggtgtggtc
     9961 ggcacattgt tggcggttgc gctgatcctg attctggtca acccgaaaac cgaccttgcc
    10021 gcatttggtc tgatgaaaga cattcctgcc gattacaaca atatccgcga tttgggcagc
    10081 cgtatttata ccgactatct gttgccgttt gaattggcgg cggtattgct gttgttgggt
    10141 atggtggcgg cgattgcgct ggttcaccgt aaaaccatca atccgaaacg tatggatcct
    10201 gccgaccagg ttaaagtacg cgccgaccaa ggccgtatgc gtctggtgaa aatggaagcg
    10261 gtcaaaccgc aagtcgaatc tgccgaagaa agcgaagttt cagacggtct caagccggaa
    10321 ggggagggca aagcatgatt accttgacgc attatctggt attgggcgcg ctcctgttcg
    10381 gtatcagcgc gatgggcatc tttatgaacc gcaaaaacgt gctggtcttg ctgatgtcca
    10441 tcgagctgat gcttttggcg gtgaacttca actttatcgc cttctcgcaa catttgggcg
    10501 ataccgccgg acagattttc gtattcttcg tactgaccgt tgccgctgcc gaatctgcca
    10561 tcggtttggc gattatggta ttggtgtacc gcaaccgaca aacaatcaac gttgccgatt
    10621 tgaacaaact taaaggttga tttggaattt ttaaagaaaa aaggagatag ataatgtttg
    10681 atattgctgc tgttggatta ggtgcagcag gagttagctt aatcaaacag ttacaggtaa
    10741 aaactcttgt ttgccatatc aaggcacttt ggtctccaca cgcccatata tgcataaatt
    10801 gtggattagt atgagtgaaa gagaatgttt acaatttcaa gggctcattg gcagccattg
    10861 ggctgtatgg cgggtaattg ggtaaaacaa tgggtttttg aaaattttag aatttcagaa
    10921 agtggagagt gattatgtca ttttcaattg ctcttccatt ggtatatctg tttattggca
    10981 ttttgattgg gaaaacccct tttagaaaaa ttaaaggcac tttttctgta atattgagca
    11041 gatggattat cccttttgtt attgtctata acataacgac atatcgtgat ggtatgcagt
    11101 atgttatgtt atggatgatt atttcgatgg ggttgattat cctaatcggg aaaagagttg
    11161 ataaagatcc tgtttggaat ttgtgtttgt gctatttgaa catcggatgg ttggggcttc
    11221 ccatagttgc aaccttgttt ggagatgagg cggcgcaagt gcttatttcg gcatacgtgg
    11281 gaagttcttt attcggaaat tcggtcgggg tcgggttgtt attgaataat agtaatttca
    11341 tatccggatt gaaaaagact ttaaaatccc ctcccgttgt tgcattggta ataggaatgt
    11401 tcggtattcc gctagggcat tttgtagcag attattttgg ggaagtttat tttttctcga
    11461 aaatagcaat gggaatattg gggatggtga tactcggtat ttggctggct gagataaaac
    11521 taaatttgaa tgatttgaag aaaagtatta aaccgtttct aataaaaaac attttattta
    11581 tagggattgt ttttcttttt atccaaatag ctgcatattt tgacttgaag ttgattcttg
    11641 aaaaccaaaa aacattgtat ttaataccgt tgctgccgcc tgctgccaat attattgtgt
    11701 tggaaacggc atatttgaaa acagggcgtt cggcttctat gattgcgtat ggtacgattt
    11761 ttagtttaat cgcaatcagc atttatattt tactggtgca aatttaaatg aacgacatga
    11821 ctttatatct catcatcgcc ctcgtgcctt tggcaggctc gctgattgcg ggtttgttcg
    11881 gcaacaaaat cggacgtgcc ggcgcgcata cggttacgat actcggcgtg gctgtgtccg
    11941 ccgtgctgtc ggcttatgtg ctgtggggat tcctcaatgg cagccgtgcc aagtttgacg
    12001 aaaacgtcta tacctggctg acaatgggcg gcttggattt ttccgtcggc ttcttggttg
    12061 atacgatgac ggcgatgatg atggtcgtgg taacgggcgt gtcgttgatg gtgcatatct
    12121 acaccatcgg ctatatgcac gacgaaaaag tcggctacca acgcttcttc agctacattt
    12181 ctttgtttac attcagcatg ttgatgctga ttatgagcaa caacttcatc cagctcttct
    12241 tcggttggga agcggtgggt ttggtgtcgt atctcttgat cggtttctat ttcaaacgcc
    12301 cgagcgcgac atttgccaac ctgaaagcct ttttgatcaa ccgtgtcggc gacttcggct
    12361 ttttgctcgg tatcggcttg gtgcttgcct atttcggcgg cagcctgcgc tatcaggacg
    12421 tattcgccta tctgcccaac gtccaaaatg ccaccatcca gcttttcccc ggcgtggaat
    12481 ggtctttgat taccgtaact tgtttacttc tgtttgtcgg tgcgatgggt aaatcggcac
    12541 aattcccgct gcatgtctgg ctgcccgatt cgatggaagg cccgaccccg atttccgcat
    12601 tgattcacgc cgcaaccatg gttaccgccg gtctgttcat ggtatcgcgt atgtcgccga
    12661 tttatgagat gagcagcact gcgctgtctg tcatcatggt aatcggtgcg attactgctc
    12721 tgtttatggg tttcttgggc gtgattcaaa acgacatcaa acgtgtggtt gcgtattcca
    12781 ccctgtcgca actgggctac atgaccgtgg ctctgggcgc gtctgcctat tccgtggcga
    12841 tgttccatgt gatgacccac gccttcttta aagccttgct gttcttggcg gcgggcagcg
    12901 cgattatcgg tatgcaccat gaccaagata tgcgccacat gggcaatctg aaaaaataca
    12961 tgccgattac ttggctgacc atgctgatcg gtaacttgtc gctgatcggt acgccgttct
    13021 tctccggctt ctattccaaa gattcgatta tcgaagcggc gaaatacagc accctgccgg
    13081 gcagcggctt tgcctatttt gccgtcctcg ccagcgtttt tgttactgcg ttctacgcat
    13141 tccgccaata ctttatggtg ttccacggcg aagagaaatg gcgcagcttg cctgaacatc
    13201 atgacgatca tcatggcgaa gaacatcaca gcttgggcaa aaacgacaat ccgcacgaaa
    13261 gcccgttggt tgttaccctg cctttgattt tgcttgccat tccgtccgtc atcatcggct
    13321 accttgccat cgagcctatg ctttacggcg atttcttcaa agatgtgatt ttcgtcaata
    13381 ccgacgcgca tccgaccatg cacatcatga aggaagagtt ccacggcgca ttggcaatgg
    13441 tgtcgcacag cctgcattcg cccgtactct accttgctat cgcaggcgtg ttgagcgcat
    13501 ggcttttgta cgtcaaactg ccgcacctgc cagcgaaaat tgcacagacg ttccgtccga
    13561 tttacgtttt gtttgaaaac aaatactacc tcgacgccct gtatttcaac gttttcgcca
    13621 aaggcacacg cgcattgggt accttcttct ggaaagtcgg cgataccgcc attatcgata
    13681 acggcatcgt caacggctct gccaaactgg tcggcgcgat tgccgcgcaa gtgcgtaaag
    13741 cccaaaccgg ctttatctac acctacgccg ccgctatggt attcggcgta ttggtactgc
    13801 tcggcatgac cttctgggga ttgttcagat aaaggcggag tttcagacga cctgttcata
    13861 gttttgtaga ggtcgtctga aaagtttgag aagtgggcat taagtcaaag ggtggaacaa
    13921 aatggtaccc aacaggcgtg aaatagccaa catgcaaatt atctgtttcc taaatttttt
    13981 aggcaatctc aacaaatata gaggagtcaa aagggatgac acagttattt ttacattcag
    14041 atacaccaaa tgagaaggaa ctgatttcaa tatatgagaa agctgtttca gaggcagtag
    14101 agctgtatat tttctctgcg tatctcactc attgggatgt aaaaaataag ctcaatagca
    14161 aattgcaaga gtttaaattt atttttggac aagatttcgg attgtctaaa aaagaggcta
    14221 ttcggaaagt gttgaaatgg cttccgtccc atttgaaatt cacactgatg gtagctcaag
    14281 gcattcaggg ttttcatcct aaagctgtat tttggaagaa tgataagaat gaatattatg
    14341 ctttaatcgg ttcttcaaat ttgactcatg ctgcgtttaa tagcaattat gaagcaaata
    14401 ttttgactaa aatttccgag caggatttta taaaagtcaa atcttgggct gatgaaattg
    14461 cgatgaaatc tatcccagta tcggaagatt ggcttgagga gtatcaggaa gctgaaatta
    14521 attataagaa atcaactgtt agccaatcat tgatggataa gttatttatg gaaatgccaa
    14581 attataatca agaactgatt gcagcacgga gaaaacagat gagaaaccat caaactgtgt
    14641 gtaatcaatt aaaaagtctt attaagcaat gtgctgcagg aaagatagac aatgatgagt
    14701 tttatgtgga atttaataaa ttatggtctt ggaaaagcga aaataaagga gaaggggtag
    14761 gtaatcgatt tcaagatcaa acgtggaaaa ggaccggaaa gtcttcagat tttagaaaat
    14821 tatgcattgc tatccaatca gtttttgatg caccacttgc tgagagagat aatgttgttg
    14881 ccaagcaaat tgattggcta aaagaatgtg gagtttccac tcgtggttca gttttttctg
    14941 aaatgctttg tcaagaatat ccagatagat atccagtgtt aaacgctcct attaaaaaat
    15001 ttttagaaaa aaataaattt aagtctgcaa aaggagcaag tgaaggctct agatatattg
    15061 atttaagtat gaagttaaga gctttactag caatgcagtc agaaataaaa aatttggctg
    15121 aattagatgt tttagtgcag gcagaatatc gcgatagtac agatattgat tgggagtgag
    15181 taaagagggg agagagtatt catccctttt tcagacgacc tcctgaaacg tcgtctgaca
    15241 aactaacccg cactacgata tttttatttt aaccacaggt taaccactat gttttccaac
    15301 tacctactca gcttggcaat atggataccc atcgccgcag gtgtgctggt tttgtcgacc
    15361 ggtaaggaca gccgtgcgcc gctggcgcgt gtgcttgcct ttatgggtgc gcttgccggt
    15421 ttcttggtaa cgctgcccct gtttaccggt ttcgaccgtt tgagcggcgg ctatcaattt
    15481 accgagttcc acgagtggat tccgctgctg aaaatcaact atgcattggg cgtggacggt
    15541 atttcagtgc tctttatcat cttgaatgcg tttattacgc tgctggtggt attggccggt
    15601 tgggaagtca ttcagaaacg tccggcgcag tatatggccg ctttcctgat gatgtcgggt
    15661 ttgattaacg gcgcgtttgc cgctcaggat gcgattctgt tttatgtgtt ctttgagggt
    15721 atgctgattc cgctgtacct gattatcggt gtatggggcg gcccgcgccg cgtctatgcg
    15781 tcggtcaagc tgttcctcta tacgctgatg ggttcactct tgatgctggt tgccatggtt
    15841 tacctgtact atcaaacagg cagcttctct attgtcgatt tccaaaacat caaacagatt
    15901 ccgttgggcg tacagcagct tttgtttgtg gcattcttcc tgtcatttgc cgtaaaagtg
    15961 ccgatgttcc ccgtacacac ttggctgccg gatgcccacg ttgaagcacc gaccggcggt
    16021 tcgatggtgt tggctgcgat tacgctgaaa ctgggtgcgt atggtttctt gcgctttatc
    16081 ctgccgatta tgccggatgc ggcacgctat tttgcccccg tgatcatcgt attgagtctg
    16141 attgccgtga tttatatcgg tatggtggct ttggtgcaaa ccgatatgaa aaaactggtg
    16201 gcgtattcgt ccatcagcca tatgggtttt gtaacgcttg ggatgttttt gtttgttgac
    16261 gggcagttgg acgactgggc attgaaaggc gcggtcattc agatgatttc gcacggtttc
    16321 gtgtcggcgg cgatgtttat gtgcatcggt gtgatgtacg accgcctgca cacgcgcaat
    16381 atcgccgatt acggcggcgt ggtcaacgtg atgcccaagt ttgcggcgtt tatgatgttg
    16441 ttcggtatgg cgaacgccgg tttgccggcg acttccggct tcgtgggcga gtttatggtg
    16501 attatgggcg cggttaaagt gaatttctgg gtgggcgcgt tggcggctat gacgctgatt
    16561 tacggcgcgt cttataccct gtggatgtac aaacgcgtga ttttcggcga gattcgcaat
    16621 ccgcacgttg ccgatatgaa agacatcaat tgccgcgaat ttgcggtttt ggcggttttg
    16681 gcggcggctg ttttgggtat gggcctgtat ccgaacgcgt ttatcgaagt ggtacatcag
    16741 gcggcaaacg atttgattgc ccatgtggca caaagcaaga tttgaggtgt gtaaatgaac
    16801 tggtctgatt tgaatttaat gcccgccatg cccgaaatcg tgctgctgtc gctgctggtg
    16861 ttattgttgc tggcagactt gtgggtcagt gatgacaaac gccgttggac gcattacggc
    16921 gcgttggcaa cggtggcggt tacggctgtg gtgcagcttg ccgtttggga acagggcagc
    16981 acgtcttcgt tcaacggcat gtatattgca gacggtatgt cgcgtttggc aaaaatggtt
    17041 ttatatgcct tgacctttgc cctgtttgtc tatgccaggc cctacaacca agtgcgcggt
    17101 attttcaaag gcgagtttta caccctgtcg ctgtttgccc tcttgggtat gggcgtgatg
    17161 gtgagcgcgg ggcatttttt aactgcctat atcggtttgg aactcttgtc gcttgccctt
    17221 tacgccctga ttgccctgcg ccgcgattcc ggctttgccg ccgaagccgc cttgaaatat
    17281 tttgttctag gcgcgctggc atccggcctg ctgctctacg gtatttctat ggtttacggc
    17341 gcaaccggtt cgctggaatt tgccggcgtg cttgcctctt ccttcaatga agaagccaac
    17401 gaatggctgt tgaaactggg tctggtgttt atcgtcgtcg ccgttgggtt caaactcggc
    17461 gcggtaccgt tccatatgtg ggtgcccgac gtgtatcacg gcgcgcccac ttctgttacc
    17521 gccctagtcg gcactgcccc gaaagtcgcc gccgtcgttt tcgctttccg catcctcgtt
    17581 accgggctgg gaaccgtgca tcatgactgg tctctgatgt ttgccctgct tgccgccgcc
    17641 tcgctgctgg tcggcaacct tgccgccatc atgcagacca atatcaaacg tatgctcgcc
    17701 tattccaccg tatcgcatat gggtttcatc ctgttggcgt ttatggcggg cgcggtcggc
    17761 tttgcggcgg gtctctatta cgccattacc tacgcgctga tggcggcggc agggttcggc
    17821 gtgttgatgg tgttgtcgga tggggacaac gagtgcgaaa acatcagcga tttggcaggg
    17881 ctgaaccaac accgcgtatg gcttgccttt ttgatgttac tggttatgtt ctctatggcg
    17941 ggcattccgc cgctgatggg tttttacgcc aaatttggcg tgattatggc actcttaaaa
    18001 caaggctatg tttggttgtc ggtgtttgcc gtcgtgatgt cgctgattgg cgcgttctac
    18061 tacctgcgcg tggtcaaagt catgtatttt gacgaatccg gccgcgcccg tctcgccgcc
    18121 ggcggcaaca atgccgcaaa attcctcttg agcgtcaacg cgctgctgtt ggtgctgtgg
    18181 ggcattatgc cgcaaaccgt tatcgactgg tgcgccaagg cgttggaaaa cacgctgtaa
    18241 gccgccgcaa cggcagccgt gtcagaggct gccgtttttg ttaagatatg ccgttccgca
    18301 atgcagttca gacggcatcg ccgccgacaa cgcccaaaca gaaagcccac catgaccgca
    18361 tccatgtaca tccttttggt cttggcactc atctttgcca acgccccctt cctcacgacc
    18421 agactgttcg gcgtggccgc gctcaagcgc aaacatttcg gacaccactt gatcgagctg
    18481 gcggcaggtt tcgcgctgac cgccgttctt gcctacatcc tcgaatcccg tgcgggagcg
    18541 gtacacaatc agggttggga gttctacgcc accgtcgtct gcctgtacct gatttttgcg
    18601 tttccctgtt tcgtgtggcg gtatttttgg cacacgcgca acagggaata ggcaggcata
    18661 ggaatgccgt ctgaaaccct ttcagacggc atttgtttca ttcaagtgca ggccggcatc
    18721 gctgtgccgg cacgtttcag ccggcgatat acgccggttt taatatttgc gggcgactgc
    18781 aaattctgcc aactgccgca ggcgcagggc tttgtcgccg aagggttcga gcagcgcgac
    18841 cgcttcggca accagtttgt gtgcgtatga gcgcgccgct tccaagccca tcagtttcac
    18901 ataagtcggc ttgtcgttgt ctgcgtcttt gcccgccgtt ttgcccaaag tcgccgtgtc
    18961 cgcttcacaa tccaacacat cgtcaatgac ttggaacgcc agccccagtt ttgccgcata
    19021 atcgtccaat accgccagac tgtcttcaga cagattcggg caggacatcg cccccaataa
    19081 aaccgccgca cggattagcg cacccgtttt caggctgtgc atctgttcca aatcggcttg
    19141 aaccatttgt ttgccgacat tcgccaaatc gattgcctga ccgcccgcca tgcccgcgct
    19201 gccgcccgct ttcgccaaca ccgacaacat tgccaactgg cgtgctgcgg gcagttctgt
    19261 cggacggctt aacacgtcga aggcttgggt ttgcaaagcg tcgccgacca gtagcgcggt
    19321 ggcttcgtcg tattgtatat ggcaagtcgg tttgccgcgc cgcaggctgt cgttgtccat
    19381 cgccggcata tcgtcgtgaa ccaaagaata gacgtggatc atttcgattg ccgccattgc
    19441 ctgtccgacc gcatccgcca cggctccgcc caattccgaa gccgccagca ccagcatcgg
    19501 acgcagacgt ttgcccccgt ccaaagccgc ataacgcatg gcttcgtgca gtgtgtgcgg
    19561 tatttccccc tcagacggta aaaaccgttc aagcagcagc tctgtttgcg cctgcgccct
    19621 aagctgccat gctttcaaat cattcgtcgg attcaaggtt caactccttc agcccgtccg
    19681 catccaaaac ctgtaatttc tgttcgactt gtgccagttt ggtttggcag tacctgacca
    19741 gctcgttgcc ttcctgatag gcggcaagcg cgtcttccaa gggcatttcg ccctgcatag
    19801 actgcgtcag cgattcgagg cgcgacaagg cttcttcaaa cgatttcggg gcgtttttct
    19861 tcatcgtatt tccttttcgg ttgaaacccc gccctttagg gcggcaggat cagactttat
    19921 ttgggagggg tgtaaccctt tccaaatcag ggcaatacat agggcggtgc tttatgtgcc
    19981 gtcctgtgtg ttggaacata gtttcggatg ttccggtaaa aagcggattg tagcattttt
    20041 gaaaaacgga tgccgtctga aacccgaatc cggcttcaga cggcattttt tccgcccagg
    20101 cggcaaggcg ttacccgggc agttcgtcgg tgatgccctg caaaaaggcg aggcgttcgg
    20161 ggcttgccgc cccggtttgc gcggcggctt tgaaggcgca gccgggttcg gcgcggtggg
    20221 tgcagttgtg gaagcggcat tgcccgacaa ggtggcggaa atcggggaaa tagcgcggca
    20281 aatcggcggc ttggaggtgg tgtaaaccaa attcttgcaa acccggggag tcgatgagtt
    20341 gggtttcgcc gttcaaatca taaagccggg cgtgggtggt ggtgtgtttt cccgagtcga
    20401 gtgcggcgga aatgtcgccg gtgcgggcgg tttggctgcc caaaagggcg ttggtcaggg
    20461 tggatttgcc cataccgctc tgcccgagca ggatgttgct gtgcccttgc agggcggggc
    20521 gcaggctgcc ggcgttttcc agtgcgcggg tttcgatgac gggataaccc agcgtttcgt
    20581 agaatttgag tttttcgcgc caaagggcgg tttcgggcag gtcggctttg ttcaggacga
    20641 tgacggcttc aataccggcg gcttcggcgg caagcagggc gcgttgcagc agccgcacgc
    20701 tcggactcgg gacggcggcg gttacgatga ggagttgggt aacgttggcg gcgatgagtt
    20761 tggttttcca cgcgtcttgg cggtagagca ggctttggcg cggtaaaaaa tcttcaatca
    20821 caacttgttc ggcgttgacg gggctgatgc ggacacggtc gccgcaggcg aaatcgacgc
    20881 gttttttgcg ggtgctggct tcgtaggttg tgccgtcggg cgtgcgcacg atgtagcggc
    20941 ggccgtagct ggcggtaatt tgggcggtgt cgttcatggt ttctttgggg ttgggtgtgg
    21001 aaatgccgtc tgaaaacggg tgttcggacg gcatcggttc agtcgtgctg ccactcgacg
    21061 tgttcgttga ggaagccgcc gctctggtgc gcccagagtt tggcgtaaag cccgcgtttt
    21121 tcgaggagtt cggcgtgtgt gccttcttcg atgatgcggc ctttgtcgag gacgacgagc
    21181 ctgtccattg cggcgatggt ggagaggcgg tgggcgatgg cgatgacggt tttgccgtcc
    21241 atcattttgt cgaggctttc ctggatggcg gcttcgactt cggaatcgag cgcgctggtg
    21301 gcttcgtcga gcagcaggat gggcgcgtct ttgagcatca cgcgggcaat ggcgatgcgc
    21361 tggcgttgcc cgccggagag tttcacgccg cgttcgccga cgtgcgcgtc atagccgcgc
    21421 cgccctttgg catcggaaag ttcggggatg aagccggcgg cttcggcgcg ttcggcggcg
    21481 gaaaccattt cggcatcggt cgcgtcgggg cggccgtaaa taatgttgtc gcgcacggaa
    21541 cggtgcagca gcgaggtatc ttgcgtgacc aaaccgattt gggcgcgtaa agattcttgg
    21601 gtaacgccgc ttatgtcctg cccgtcgatc gaaaccgtgc cgctttgcgg ttcgtagaag
    21661 cgcaaaagca ggttgacgat ggtggatttg cccgcgccgc tgcgtccgat caagccgact
    21721 ttttcgcccg ggcggatggt gaggttgaag ccgttgagca gcggtttgcc ggcttcgtag
    21781 cagaaatcga tgtgttcaaa tttgatttcg ccttgcgaca ctttcagcgg cagggcgttc
    21841 ggcttgtcga ggatggtgtg cggtttggac agggttgcca tgccgtcgcc gacggtgccg
    21901 atgttttcaa acagccgcgc ggattcccac ataatgtatt gcgacagccc gttgacgcgc
    21961 aacgccatcg cggtggcggt ggcgaccgcg cccacgccga cctgcccgtt gtgccagagc
    22021 cagatgccca gtgcggcggt ggagagggtc agggaggtgt tgacgatgaa gctgcacgaa
    22081 tgcagcagcg tcgccagccg catttgggcg cgcaccgtaa ccataaattc ttccatcgac
    22141 tgcttggcat aggcggcttc gcgcgcgccg tgggagaaga gtttgacggt ggcgatattg
    22201 gaataggcat cggtaatgcg gccggtcatc agcgagcggg catccgcctg ccatgcggcg
    22261 gtttgcccca atttgggaat cagcaggcgc atcaccgaag cgaaaccgac aatccagccg
    22321 ataaagggca gcagcagcca tgagtcgagc gaggcgagaa tcacgccgga ggtaatgaaa
    22381 tacaccgaca cataaacgac catatcggca accgtcatca ccgcgtcgcg caacgccagc
    22441 gcggtctgca tgactttggc ggacacgcgt ccggcaaatt cgtcctgata aaaaccgagg
    22501 ctttggttca gcatcaggcg gtggaagttc cagcgcaggc gcatagggaa cacgccctga
    22561 agggtttgca ggcgcacgtt ggacgcggca tacgcccaca cgaccgaaaa caccatcatc
    22621 gccgccattg ccgccagttc ccaacctttt tcggcaaaca gttcggcggg cgcgtatttg
    22681 ccgagccact ccacgatttt gcccataaat tgaaaaacca gggcttccat aatgccgatg
    22741 ccggcggtca gcgcagccag ggcggctatc catttccgca cgccggccat gctgctccag
    22801 acaaaccgcc acaagccttt ttctggcgtt ttcggggcgg cttcgggata agggtcgatt
    22861 cgggactcga accaggaaaa tattttgttc aacattgttt tcgatttcgg taaaacagtt
    22921 tcagacggca tcaaacacaa tgccgtctga aaggaaggac aataacgcca ttttacggga
    22981 aaagccgtcg ggaagacagc gcgaggcgga aacgcagggt ttcgtcaggg caaacgccgc
    23041 gccgccttca gacggcatta tttcagcagg tttttcaaag caaggcgcac gccttcgccc
    23101 acgtccgtcc ctttcggaac gcctttgacc gccgcttttg cttcgcgttc gctgtaaccc
    23161 agtgcaagca gtgtgctgac gatgtcttcc gtttcgtcgg cggcgggtgc ggcggcaaac
    23221 agcccgtccg ttaccgtatg cgcgaccagc ttgccgcgca gttccaagac catacgttcg
    23281 gcggtttttt tgccgattcc cggcgcggaa gacaggcgtt tgacatcttc ttctgcaacc
    23341 gcccgcgcca gctcgtctgc cgtcatcgct gacaaaatgc ccaaagccgt tttcgcgccg
    23401 atgccgccga ccttaatcag ttggcggaag gtcttgcgtt cttccgcagt ggcaaaacca
    23461 aataaaagat gtgcgtcttc ccgaatgata agctgggtaa acagttgtac gctttcaccc
    23521 acgggcggca ggttgtagaa ggtctgcatc gatacgtcgg cctcatagcc gacaccgttg
    23581 acatcgatga cgatttgcgg agggtttttt tcaaccagtt tgccggtcag tctgctgatc
    23641 atgtatgaag gatcctgaag tgtcgggtgc aaaatgccgt ctgaaaccgg ttcgggcttc
    23701 agacggcacg gattgtatca aattcagtcg tcgcggcggg aggaaatcac gcggccggta
    23761 cgggcatcga caacgacttt gtattcctgt ccgtttttga cgatttcgac atcatagtgc
    23821 ggacggccgt tgtcgtgttc gagatcgatg tcggtgattt tgccgccgac acgcgccaac
    23881 gctgcttttt cggcttgggc gcggctgatg attttgtctt gtttgctgtg ttggtgtgcg
    23941 gcgtgtccgt ggtcgtcatc gccgtgtccg tcgtggtggg cgagtgcggg ggcggaaatg
    24001 cccatcagtg cggttgcggc ggcggtcaag agaaggtgtt tgatgttcat attttgcctt
    24061 tgtaaatcgt gggttggaaa atgtggatat taataaggta tcaaataacc gtcagccggc
    24121 agtcaatacc gcccgaacca taccgcgcgc ctgagcttcg gcttcggcgg cgcgttcctg
    24181 cgaggaaaac ggccccattt tgacgacata ttcgtaatgg cgtttttcaa ccgataggtt
    24241 cgggtttgat gccgatgaag ccaggttttg ggcggcttgg ttcagatagg cttgtgcttc
    24301 gtgttccgta ccgaaagatt tcaagtcgat gaagatgtct ttgttttcgg caaccagtgc
    24361 ggattggccc gggacgattt gttcgatttt gacgtgcgcc gtcccttggc tgacaaagcc
    24421 caatttttgc gcggcggctt tggatacgtc gatgatgcgg ttgccgtgga agggaccgcg
    24481 gtcgttgacg cggacgatga cgcttttgcc gtttttggta ttggttacgc gcacatagct
    24541 ggggatgggc agggttttgt gggcggcggt aaaggcgttc atatcgtatc gttctccgcc
    24601 ggaagttttg cgcccgtgaa acctgccgcc gtaccacgag gcgttgccgg tttgcgtgaa
    24661 ttcggcgact tggtttttcg gcgtgtagcg ttttccggcg actttgtagc tgcggttggc
    24721 ggaggcgtgc agtttttctg ccttgaccac tgcgtcggcg gatgccgtct gaagggagtg
    24781 tgtgccgaat gcggcggtga gaaggaaaag ggtttttcgg gttaaagtca aaacgtgttc
    24841 cgttcttgag ttgaagacga atgggcatca tgcccgccgg atacgttccg aaccgccgta
    24901 cagtgcggac ggcggttcgg aatgtgtccg gataggtttt cagacggcat gaacttgcgt
    24961 tcaaacgccg cctgcgtaac cgtgttgccg ccacgcttca aagagaatca cggcaacggt
    25021 attggaaagg ttcatactcc ggctgccggg ctgcatcggc aggcggattt tttgcgcggc
    25081 gggcaggctg tcgaggatgt cggcaggcag tccgcgcgtt tccggcccga acagtaaaac
    25141 atcgcctttc tgaaacgcgg tttcatcggg gcgcgccgtg cctttggtgg tcagggcgaa
    25201 aatgcgcctg cctgcgagtg ccttgaggca gtcgtcgaag ttttcgtgca ccgtcaggct
    25261 ggcgaattcg tggtagtcga gcccggcgcg tttcattttg gcggaatcca atgggaagcc
    25321 gagcggtttg acaaggtgca aatccgcgcc ggtattggcg cacaggcgga tgatattgcc
    25381 cgtgttcggc gggatttccg gttggtataa aacgatggta aacataaata tcaatcactt
    25441 ataggcgcgt aaccttgcca caaggcggat ggggtgtcaa aaaatttagt tattttttca
    25501 ttggcgtgcg tgccagcgtc cagcagcaga ttcggtttgc gcccgatttt ttcagcgtct
    25561 ttgccagttc gtccagcgtc gcgccggtgg taaagacatc gtcgattaac aaaatattac
    25621 agttttccgg tatcggtgtg cggatttcaa aggcgttttt gatgtttcgc cgccgttcgc
    25681 cgcctttgag cgtgctttgc ggcgggcggt ggtgtcggaa aacggtgtgt cggggcagta
    25741 tctgccagcc gtagcgttgt gccagcagcc cgacgatgct ttcactttgg ttgaacccgc
    25801 gttgcagcag ccgctccctg cttagcggta cgggcaggac gaaatcgaaa cattcgtctg
    25861 caagccggtc gggcggattc tgcatcatca ggtctgccag cggctgcacc atgctcaaat
    25921 cagccaagtg cttcagcgcg tgtatcatat tgctgacggg cggttcgtaa tgcagcgaag
    25981 cccacatccg gtcgaatgcg ggcggttttt tctgacagcc gccgcacacc gcgccgcctt
    26041 gaacgtgcat gaaacacagg gggcagctgt ttgccgcgtc ggtgcggtat gccgccaaat
    26101 cgtcgcggca gccggcgcag atgccgtctg aaacgccgga cgaaccgtgg cataatacgc
    26161 aacgcctgat agtgggcgcg tctgcgatgc gccgccaaca agagagaaaa tccatgcctg
    26221 atgccgtcaa aaaagtttac ctgatacacg gttggggggc gaaccgccac gtgttcgacg
    26281 atttgatgcc gcgcctgcct gcaacgtggc cggtgtccgc cgtcgatttg cccggacacg
    26341 gcgacgcgcc cttcgcgcaa cccttcgata ttgaggcagc cgccgacgct gttgccgcac
    26401 aaatcgatac gcccgccgac attctcggct ggtcgctcgg cggattggtc gcgctgtatc
    26461 tggcggcgcg ccatcccgac aaagtccgtt cgctctgcct gacggcgagt ttcgcacggc
    26521 tgacggctga cgaagactat cccgaagggc ttgccgcgcc tgcattgggc aaaatggtcg
    26581 gcgcgttccg aacggattat gccaaacata tcaaacagtt tctacaactc cagcttttgc
    26641 acacgcctga tgcggacgga atcataggca gaatcctgcc cgatttggcg cgctgcggca
    26701 cgccctccgc cttacaggaa gctttggacg cggcggaaag ggcggatgcg cggcatttgt
    26761 tggacaagat agatgttccg gtactgctgg tgttcggtag caaagacgcg attacgccgc
    26821 cgcgcatggg cgagtatctg caccgccgtt tgaaaggcag caggctggtt gtgatggaaa
    26881 aggcggcgca tgcgccgttt ttgagccatg cggaagcgtt tgccgcgctg taccgcgact
    26941 ttgtcgaagg ggtttgacat gaatccgcag gacaaatgct ggcaggttca ccgccatctc
    27001 gccgaatatg ccgaccaacg cctgacactc gtccgcaacg cgcccaagta tatcctgctt
    27061 gcaggtgcgg atgcggacat cagccgcagc ctgctggcga aacgctatcc gcaggcggta
    27121 tttgaagaat atgattcccg tgcggatttt ttggcggctg ccgctgccgc ccgcaaaggc
    27181 ggtttttggc aaaggtttac gggtaagggc gtggtgcaac actgccaatc cccgatcgcg
    27241 ccgctgcccg aagcgtgtgc cgatatgttg tggtcgaatc tcggactgtt ggcggcggaa
    27301 caaatccttc ctgtgctgca caactgggcg cgcgccttga agacggacgg gctgctgttt
    27361 tttacctgct tcgggcgcga cacttttacc gaactgaaat gccgtctgaa agaaaacggc
    27421 attgaaagcc gcagcgtgat gtttcccgat atgcacgact tgggcgatat gcttgctgaa
    27481 aacggctttt acgaccccgt taccgatacg gcgaagctgg tgttggatta caaaaaggcg
    27541 gaaacgtttt gggcggatat ggacacgctg ggcgtttggc gggcgatggc gtggaacgat
    27601 gaaaacgccg cgcgttcgtg tgtcgggaca atatttgaac gggaaggcgg tttgagtatt
    27661 acgctggaaa cagtgtacgg acacgccgtt aaaaaactga tgctgccgca aggggagaac
    27721 gtggtgcagt tttttccgaa gagatgatgt gcagatgccg tctgaagccg tttccaagtt
    27781 tcagacggca tttgtttgtg aaaaccgaca gaactaaagg aaatgccgat gtatagtgaa
    27841 ttaaatttaa accagtacag cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc
    27901 ttcgccgcct tgtcctgatt tttgttaatc cactatatgc tgatgccgga gacgtatatt
    27961 gcgtctataa catcagactg aagcagtaca ctgcctgcca ggttacccga gttgaagaac
    28021 acggtggcaa aaaaacacat gcgaccctgc tggctttgga ctggcagggc aacaaaccgc
    28081 ttggggcgga ggagctggcg gatttgaaac cactttacaa agacttaaag aataatattg
    28141 gaaatattgt atgaacaaaa aattaaacta tatttttatg ttggactgtt tagggttggt
    28201 gatattgttt acttgtataa tagctacttt tgaaagagat tatggattta aaatttttac
    28261 taattctaag aggcctgaat tttattattg gattggaatg ttttattatg gaattatttc
    28321 ttgctggttt gattatcaat taatttcaac aaaggcgaat tcgtataaaa gaaaagttaa
    28381 acaatataaa attttttcag taatattttc agttttgata tttatttcta ctatagtaaa
    28441 actttaaatt ttggagcaaa aatttatgag cgattcaatt gaatatgtat tgggaacgcg
    28501 gtctgcacat gtataaggca agtgctgtcg tgccaacggg atatgtacgg gttgggaata
    28561 ccgcgccgct ggtcggcgaa gacacgcaac ggtatgcctc tttttggggc gacggctacg
    28621 acgtgtaccg tcagttgaga tggcagcaga tacccgaaaa acagagaaag gcattcaaaa
    28681 aagccgccaa aagcaaaaag accgtgatgt ttgccggacg ggaatacggc atatccaaac
    28741 agaatttgag cgatgtttgg gatgattttg aagacgcgat ggaactgaag gcgtttccct
    28801 gcctgtcttc gctgtttctg accaaatggc ataaaaatct atatgatagt ggattaacaa
    28861 aaaccagtac agcgttg
//
LOCUS       DAWHXZ010000003        25654 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000003 DAWHXZ010000000
VERSION     DAWHXZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25654
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.003"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            194..1036
                     /locus_tag="WIE35_000089"
     CDS             194..1036
                     /locus_tag="WIE35_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ4153081.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     NDRVAVIATLPLTRDESWSQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
     gene            1065..1529
                     /gene="nrdR"
                     /locus_tag="WIE35_000090"
     CDS             1065..1529
                     /gene="nrdR"
                     /locus_tag="WIE35_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ4153082.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPNCGKRFGTLETA
                     ELKMPAVIGTDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSTELADMVLKELLEHNTEAAVRFAALHKRFDNPSDFASWLAQAVKTDRKA"
     gene            1562..2671
                     /gene="ribD"
                     /locus_tag="WIE35_000091"
     CDS             1562..2671
                     /gene="ribD"
                     /locus_tag="WIE35_000091"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ4153083.1"
                     /translation="MFSDTDISMMEIALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLAMLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPQLNVRDFPTLRQP
                     ARIVLDSRLRLPPYSHLVTDGQSPTLIATLERDEDKLRPYREHAHIRILMPSETADSK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGSGKDLLSLP
                     ENRAALSAPPLWTPVSTEILGHDIKTVFRKNDNAF"
     gene            2866..4284
                     /locus_tag="WIE35_000092"
     CDS             2866..4284
                     /locus_tag="WIE35_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ4153084.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSSQLVSKLAILLLLLTV
                     GLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIPI
                     ALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFRA
                     IEENAPPARLSATAEFAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPPL
                     FCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACATSFWL
                     FFAFKTESSCRLWQPLKRLPLYTHTLLCLTSSAAYTCFGTPANYPLFAGVWAAYLAGC
                     ILRHRKDLHKLFHYLKKQGFPL"
     gene            4281..4865
                     /locus_tag="WIE35_000093"
     CDS             4281..4865
                     /locus_tag="WIE35_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ4153085.1"
                     /translation="MKIVLTTSMAGLGGTETATVRLGRLLRQHGHDIMLASSDGPLLK
                     EAQALGIRWQPVDFYRGGLFGYLKGMIAYARMLRREQPDIIDCQMARVVPACAIAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPADRIVYAY
                     NALPPPRNFLSGKPSKAASCSARCRVWTPSAPCI"
     gene            4817..5353
                     /locus_tag="WIE35_000094"
     CDS             4817..5353
                     /locus_tag="WIE35_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ4153086.1"
                     /translation="MLGTLSRLDTVRAVHLMLDIFKKMVDRDMPVRLNVAGIGEEMDN
                     LKAQAERLGIDGKVTFLGGVRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILEAGLYD
                     TPVVTYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPLLREAMGKRLHAHVA
                     ALCSDDEIYRTTMAAYTM"
     gene            5373..6540
                     /gene="pglH"
                     /locus_tag="WIE35_000095"
                     /pseudo
     CDS             5373..6540
                     /gene="pglH"
                     /locus_tag="WIE35_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
     gene            6533..8110
                     /locus_tag="WIE35_000096"
     CDS             6533..8110
                     /locus_tag="WIE35_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEZ4153087.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLILAYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYI
                     DHYSFWLDMKILFLTVRKVFIKEGISAEGEATMPYFTGNQTNEKNNILILSAGRRVEL
                     VQDFQTEAARFSDGISVFATDLNPRMSSACHVSDGSFEVPPISTDSYIDSIFNLAVRE
                     NIGLIIPTIDTELQKLADERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRS
                     PEIYDRKKLVFPCFAKPYDGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFS
                     EFTVDMYYDRQCRLKCAIPRKRLEVRSGEISKGVTCKNSLYQTLLEKMAVLQGARGCI
                     TAQFFYNEETGEFYGVEINPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWEN
                     NLIMLRYDAKVLVHEND"
     gene            8097..8783
                     /locus_tag="WIE35_000097"
     CDS             8097..8783
                     /locus_tag="WIE35_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ4153088.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDGKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSLQ
                     DLVPIIDSNT"
     gene            8831..9025
                     /locus_tag="WIE35_000098"
     CDS             8831..9025
                     /locus_tag="WIE35_000098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153089.1"
                     /translation="MTKYYSWGVNGFPPYVFDTIWKRWKLNGRGGGYRVRHPGRHDRR
                     GQSGKAAAAQKPRDRDGISD"
     gene            9149..10324
                     /locus_tag="WIE35_000099"
     CDS             9149..10324
                     /locus_tag="WIE35_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ4153090.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECRKFEKEF
                     AAFAGTRYAVALSNGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAIIVVHLAGMPAEMDGIMALAKEHNLWVVEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKELWSKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTVCRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKSFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTDGEIAFCKEHIEAVLTEATR"
     gene            10372..12282
                     /gene="pglD"
                     /locus_tag="WIE35_000100"
     CDS             10372..12282
                     /gene="pglD"
                     /locus_tag="WIE35_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ4153091.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTAPLTIGLFVRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGQAGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDNRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAVEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVETFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPAFEKQIAEGGPITLTHQDITRYFMTIPEAAQLVIQAGAMGKGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADEAMLPWHELSVLLDRIRAACDRYDQQAIRTLLINAPTGFTPSDGICDL
                     LWVRETHRKNAV"
     gene            12338..13630
                     /locus_tag="WIE35_000101"
     CDS             12338..13630
                     /locus_tag="WIE35_000101"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ4153092.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     AEVNAVFADVFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDSEMARLD
                     ALAQEHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTR
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(13682..14518)
                     /locus_tag="WIE35_000102"
     CDS             complement(13682..14518)
                     /locus_tag="WIE35_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ4153093.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYAFLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RH"
     gene            complement(14914..15117)
                     /locus_tag="WIE35_000103"
     CDS             complement(14914..15117)
                     /locus_tag="WIE35_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153094.1"
                     /translation="MDMTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(15095..15328)
                     /locus_tag="WIE35_000104"
     CDS             complement(15095..15328)
                     /locus_tag="WIE35_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ4153095.1"
                     /translation="MEAIVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            15866..19300
                     /gene="dnaE"
                     /locus_tag="WIE35_000105"
     CDS             15866..19300
                     /gene="dnaE"
                     /locus_tag="WIE35_000105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ4153096.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPVGAADVWIGNPNAPDKPFRAMLIIRNDAGYQRLSELLT
                     EAYVGKDRNVHHAELNPEWLENGDNSGLICLSGAHYGEVGMNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPDQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQVAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDLPRWSESEKLAEEKTVIGFYLSGHPFGPYAQEVCQIAPTKLDR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGSQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLS
                     SHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            19578..22826
                     /gene="cas9"
                     /locus_tag="WIE35_000106"
     CDS             19578..22826
                     /gene="cas9"
                     /locus_tag="WIE35_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEZ4153097.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEEENPIRLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVANNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEK
                     QKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAER
                     FIWLTKLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKG
                     LRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSSELQDEIGTAFSLFKTD
                     EDITGRLKDRVQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIYLPPIPADKIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLVRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKECNLNDTRYVNRFLCQFVA
                     DHILLTGKGKRRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGKVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGAHKDTLRSAKRFVKHN
                     EKISVKRVWLTEIKLADLENMVNYKNGREIELYEALKARLEAYGGNAKQAFDPKDNPF
                     YKKGGQLVKAVRVEKTQESGVLLNKKNAYTIADNGDMVRVDVFCKVDKKGKNQYFIVP
                     IYAWQVAENILPDIDCKGYRIDDSYTFCFSLHKYDLIAFQKDEKSKVEFAYYINCDSS
                     NGRFYLAWHDKGSKEQQFRISTQNLVLIQKYQVNELGKEIRPCRLKKRPPVR"
     gene            22892..23806
                     /gene="cas1"
                     /locus_tag="WIE35_000107"
     CDS             22892..23806
                     /gene="cas1"
                     /locus_tag="WIE35_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEZ4153098.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QFWQHIVRQKILNQAFIADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARTLTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKAELTPGIKQHLIKTLHYQISIERQHFSTLAAIDKMVS
                     SFQAGVTDKNAKQLKLPEILPLKEYRYE"
     gene            23799..24125
                     /gene="cas2"
                     /locus_tag="WIE35_000108"
     CDS             23799..24125
                     /gene="cas2"
                     /locus_tag="WIE35_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEZ4153099.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     repeat_region   24189..25280
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=24189..24224
                     /rpt_unit_seq="attgtagcactgcgaaatgagaatgggagctacaac"
BASE COUNT         6441 a         7439 c         6314 g         5460 t
ORIGIN      
        1 gaacgaaaaa caaccaaaac cggacaagtc ggattcccgc ctgcgcggga atgacggaat
       61 cttaagtttc cgtctttgtt ttctgttttc tgttttcgag ggaataacgg ggaacaagcc
      121 gtatttcaga cggcattttc agttcggggt ataatccgaa tacttgcgac catctgaatc
      181 attgggacaa accatgtgtc aactgctggg catgaactgc aatacgccga ccgatattat
      241 gttttccttt gaaggcttcc gccgcagggg cggcattacc gaccaccatg ccgacggttt
      301 cggcatcggc tttttcgaag gcaaaggcgt gcgcctgttc catgacgaca aacccagtgc
      361 caattcgccc gtcgccgacc tcgtgcgcgc ctaccagatt aaatcggaaa acgtcgtcgc
      421 ccatatccgc aaagcatcac aaggacaaac ctcgctggcg aacacccatc cctttatgcg
      481 cgaaatgtgg ggcggctact ggctgtttgc ccacaacgga catttgattg atttttttcc
      541 cgaacagggc gaatttttcc accccgtcgg cacaaccgat tccgaacgcg cgttctgcca
      601 catcctcaac cgcctgcgca cccgctttgc cgcccgtccc gacgacgaca cgctgtttga
      661 cgcgattgcg gggctgacgc acgaaatccg caagttcggg ctgtttaact ttatgctttc
      721 agacggcatt gccctgtttg cccacgccag cacactactg cactacatcg tccgccaagc
      781 cccgttcggc aaggcgcgcc tgctcgacga cgacgtgatg gtcgattttg ccgaagtaac
      841 cacgcccaac gaccgcgtcg ccgttatcgc caccctgccg ctgacccgcg acgaatcatg
      901 gtcccaactt gccgtgaacg aactggtcat gttccgcgaa ggcaacatcg tccgacacga
      961 ccgtcccgaa aaccccgtct atatgagtgc cgaagaaggt ctggaaatcg cccgcgccgc
     1021 cggcgtatcg gtttgacgtt cagacggcat caggaagaaa cgctatgaaa tgcccgtttt
     1081 gcgcccaccc cgacacccgc gtcgccgatt cgcgtctgat ggaagaacgc aacgccgtgc
     1141 gccgccgccg ccactgcccc aactgcggca aacgcttcgg cacgctcgaa accgccgaac
     1201 tcaaaatgcc cgccgtcatc ggcacggaca aaaaacgctc gccctttaat gcacaacgcc
     1261 tccgcaacga cctgaccgcc gccgcccgaa aatccgccct gacacccgaa cagatcgacg
     1321 aaaccgtccg cctgacggaa cacaggctct acacttcggg tcagcgcgac atcccctcca
     1381 ccgaacttgc cgacatggtg ctaaaagagc tgcttgagca caacacggaa gccgccgtcc
     1441 gcttcgccgc cctgcacaaa cgcttcgaca atccgtcaga ctttgcctcg tggctggcgc
     1501 aagccgtcaa aacagaccgc aaagcctgat tctccccaac ccatactgat acggtatccc
     1561 tatgttttcg gacacagata tatccatgat ggaaatcgcc ctccgacttg ccgctttggg
     1621 acgtttttcc acttcgccca atccgcgcgt cggctgcgtt atcgcacacg gcagccaaat
     1681 tgtcgggcaa ggcttccacg tcaaagcggg cgaaccgcac gccgaagtcc acgccctgcg
     1741 tcaggcgggc gaaatggcac aaggcgcgac cgcctttgtt accctcgaac cgtgcagcca
     1801 ttacgggcgt accccgccct gcgccgaagc tctgctccgt tccggcgtaa cacgcgtcgt
     1861 tgccgccatg cgcgacccca acccgctggt tgccggcaaa gggcttgcca tgctcgaagc
     1921 agcaggcatc aagacggaat gcggtttact cgaacatcag gcaagggaac tcaaccgagg
     1981 cttcctgtcg cgcatcgaac gccgccgccc cttcgtccgc ctcaaatgcg ccgtttcgct
     2041 ggacggcaaa accgcccttt cagacggcag cagcttttgg attaccggcg aagacgcgcg
     2101 tgccgacgta caggttttgc gtgccgaaag ctgcgcggtg ctgaccggca tcggcacggt
     2161 gttggcggac aatccccaac tcaacgtccg cgacttccca accctgcgcc aacccgcgcg
     2221 catcgtgtta gacagccgcc tgcgcctgcc cccgtacagc catttggtta ccgacggaca
     2281 atctccgacc cttatcgcca ccttggaacg cgatgaagac aaactccgcc cctatcggga
     2341 acacgcacac atccgtatcc tgatgccgtc tgaaacggca gacagcaaaa tcgacctgca
     2401 ccacctgatg cgcctccttg ctgacgaagg tttcggcgaa atcatggtcg aagcaggctc
     2461 cgaactcaca tccgcatttt tggcagaaaa tctggcagac gaaatcgtcc tgtaccgttc
     2521 gcccaaaatc ctcggcagcg gcaaagacct gctttccctg cccgaaaacc gcgccgccct
     2581 ttccgcaccg cccttgtgga cacccgtttc caccgaaatc ctcggacacg acatcaaaac
     2641 cgtgttccgg aaaaacgata acgcctttta aagggtttgc gccgtttcac tatataataa
     2701 cgccgataaa aaacggcccc gttcagaccg ccgcccgccc ggaaaagcgc aacccggact
     2761 gccgcacccc gccggcagcc gcaacggact gaaagccgtc aaattccgat caagaaaggc
     2821 ttcagacggc acaggcagca tcccgccgcc gcccggacat caaaaatgga cacaaaagaa
     2881 atcctcggct acgcggcagg ctcgatcggc agcgcggttt tagccgtcat catcctgccg
     2941 ctgctgtcgt ggtatttccc tgccgacgac atcgggcgca tcgtgctgat gcagacggcg
     3001 gcgggactga cggtgtcggt attgtgcctc gggctggatc aggcatacgt ccgcgaatac
     3061 tatgccgccg ccgacaaaga cactttgttc aaaaccctgt tcctgccgcc gctgctgtct
     3121 gccgccgcga tagccgccct gctgctttcc cgcccgtccc tgccgtctga aatcctgttt
     3181 tcgctcgacg atgccgccgc cggcatcggg ctggtgctgt ttgaactgag cttcctgccc
     3241 atccgctttc tcttactggt tttgcgtatg gaaggacgcg cccttgcctt ttcgtcctcg
     3301 caactcgtgt ccaagctcgc catcctgctg ctgctgctga cggtcgggct gctgcacttt
     3361 ccggcgaaca ccgccgtcct gaccgccgtt tacgcgctgg caaaccttgc cgccgccgcc
     3421 tttttgctgt ttcaaaaccg atgccgtctg aaggccgtcc ggcgcgcacc gttttcatcc
     3481 gccgtcctgc atcgcggcct gcgctacggc ataccgatcg cactaagcag catcgcctat
     3541 tgggggctgg catccgccga ccgtttgttc ctgaaaaaat atgccggcct agaacagctc
     3601 ggcgtttatt cgatgggtat ttcgttcggc ggggcggcat tattgttcca aagcatcttt
     3661 tcaacggtct ggacaccgta tattttccgc gcaatcgaag aaaacgcccc gcccgcccgt
     3721 ctctcagcaa cggcagaatt cgccgccgcc ctgcttgcct ccgccctctg cctgaccggc
     3781 attttctcgc ccctcgcctc cctcctgctg ccggaaaact acgccgccgt ccggtttatc
     3841 gtcgtatcgt gtatgctgcc tccgctgttt tgcacgctgg tagaaatcag cggcatcggt
     3901 ttgaacgtcg tccgaaaaac acgcccgatc gcgctcgcca ccttgggcgc gctggcggca
     3961 aacctgctgc tgctggggct tgccgtaccg tccggcggcg cgcgcggcgc ggcggttgcc
     4021 tgtgccacct cattctggtt gttttttgcc ttcaagaccg aaagctcctg ccgcctgtgg
     4081 cagccgctca aacgcctgcc gctttatacg cacacattgc tctgcctgac ctcctcggcg
     4141 gcctacacct gcttcggcac accggcaaac taccccctgt ttgccggcgt atgggcggca
     4201 tatctggcag gctgcatcct gcgccaccgg aaagatttgc acaaactgtt tcattatttg
     4261 aaaaaacaag gtttcccatt atgaaaatcg ttttgaccac atctatggca ggcttgggcg
     4321 gcacggaaac cgccaccgtc cgcctcggca ggctactcag gcagcacgga cacgacatca
     4381 tgcttgcctc ttcggacgga ccgttgctca aagaggcgca agccttgggc atacgttggc
     4441 aacccgtcga tttctatcgc ggcggccttt tcggctacct caaaggtatg attgcctacg
     4501 cccgaatgct gcggcgcgaa caacccgaca tcatcgactg ccaaatggcg cgcgtcgtac
     4561 ccgcctgcgc cattgccgcc aaaatcgttt cgccgaaaac caaaatcatc taccactcgc
     4621 acggtttgga cgcggcaacc taccccaaaa tcgccaaact cttcgacaag ctgggcgtgt
     4681 acatcatcgg taactgcaaa cacgaacgcg aaaaactcat ccgccacggt ttccccgccg
     4741 accggattgt ctacgcctac aacgcactcc cccccccccg gaatttcctt tctggaaaac
     4801 cgtcaaaggc tgcgtcatgc tcggcacgct gtcgcgtctg gacaccgtcc gcgccgtgca
     4861 tctgatgctg gacattttca aaaaaatggt cgaccgcgat atgcccgtac gcctgaacgt
     4921 ggcgggcata ggcgaagaaa tggacaattt gaaagcgcag gcggaacgtc tgggcataga
     4981 cggcaaagtt accttcctcg gcggcgtgcg cgatttgacc gcctacttca aagacgtgga
     5041 tattttggtg aacacgcccc actgcatcgg cgaccacggc gcaggtgtgg gcaacaacat
     5101 cttagaagcc ggactttacg acacacccgt cgttacctac gatatggcgg gcatttccga
     5161 aatggtcgtc aacggggaaa cgggttactg catccccttc ggcgacgaag aagcctttat
     5221 cgccgccgta gacacgctcg tcagacaacc gctgttgcgc gaagcaatgg gcaaaaggct
     5281 gcacgcacac gtcgccgcac tctgttccga cgacgaaatc taccgtacca ccatggcggc
     5341 ctataccatg taatgccgtc tgaaagccgc cgatgaacat caccatagtc gccccttatt
     5401 gctcgctgcc gtccgaaccg tatttcaacc gcttttggta tctggcagaa cgattgtcgc
     5461 aatcgcacga cgtgttgctg attaccagcc gtttccgcca ctacgacaaa tcattcagac
     5521 ggcatgaaga tgctgccgcc acttcaaacg gcaggctgcg cgtcaagctg ctggacgaac
     5581 ctggctaccg aaaaaacgtc tccctcgccc gcgtcgccag ccaccgcgta ttcgtccgca
     5641 atttggcacg ctggctgcac agcccgcagg cggcggaaca ggacatcgtc tattctgcct
     5701 atccgctgat ggcgaccaac ctgctgttgg gcaaacacaa agcgcgtttg ggctacaaac
     5761 tgattgtcga tgtgcaggac gtatggccgg agtctttctc ctcggtcgta ccatttttga
     5821 aaaaagtacc gcacaagctg ctgccctttg cttcacgcgc caaccgtgcc taccgctgcg
     5881 ccgacgcgct ggttgccgta tcgcagactt atctcgaccg cgccaaagaa gccaatccga
     5941 acgtacccgg cgaagccgtc tatatcggta cggattttgc cgccatcgcc cccccccccg
     6001 cgattccgct ccaaaaccac ccgccttttc tacttgggaa cgctcagtta cagctacgac
     6061 gtggaaaccg tgtgcaaagg tgttcggaaa cttttagacg acggcgaaaa cgtcgaattg
     6121 cacattatgg gcggaggacc cgatttggag aaactcaaac aatatgaaaa tcgcgccatc
     6181 aagttttacg gttatttacc ctattctgaa atgatgtcga ttgccaaagg ctgcgacatt
     6241 gccgtcaacg ccatccatag ccacgccatg cagtcggtta ccaacaaact ctccgactat
     6301 atggctttgc aaaaaccgat tctgaacagc cagaccaatg ccgaagtcct tagcctcttg
     6361 aacctgctgc cgcacgaaaa ctaccgttcc ggcgacgtgg acagcttcgt tcaagccgcc
     6421 aaagatattt tgaaacgcaa aaacgacccc gttcagtccg atgaaatcgt ccgccgcttc
     6481 aggcgcgaca tttcctatcg gaaaatcgtc aacctgattg aaagattggc aaatgagtaa
     6541 agccgtcaaa cgcctgttcg acatcatcgc atccgcatcg gggctgattg tcctctcacc
     6601 cgttttcctg attttggcgt acctcatccg caagaatctg ggttcgcccg tcttcttcat
     6661 tcaagaacgc cccggaaagg acggaaaacc ttttaaaatg gtcaaattcc gttccatgcg
     6721 cgacgcgctt gattcagacg gcattccgct gcccgacagc gagcggctca cacccttcgg
     6781 caaaaaactg cgtgccgcta gtttggacga actgcctgaa ttatggaatg tcttaaaagg
     6841 cgacatgagc ctggtcggcc cgcgcccgct gctgatgcaa tatctgccgc tgtacgacaa
     6901 cttccaaaac cgccgccacg aaatgaaacc cggcattacc ggctgggcgc aggtcaacgg
     6961 gcgcaacgcg ctttcgtggg acgaaaaatt cgcctgcgat gtttggtata tcgaccatta
     7021 cagtttttgg ctggatatga aaatcctgtt tttgacagtc agaaaagtct ttatcaaaga
     7081 agggatttcg gcggaagggg aagccactat gccttatttc actggaaatc aaactaatga
     7141 aaaaaataat atactgattc tgtcggcagg tcgccgtgtc gaattggtgc aggacttcca
     7201 aaccgaagcc gcacgttttt cagacggcat cagcgttttt gccactgacc tcaacccccg
     7261 catgtcttct gcctgccatg tttcggacgg ttcatttgaa gtgccgccga ttagtaccga
     7321 ttcatatatc gacagcattt tcaatctggc ggtacgcgaa aatatcggtt taatcatccc
     7381 aaccatagac accgaactgc aaaagctggc agacgagcgc gaacgctttg aagcggcaag
     7441 tgtccacatc gtcgtttccg attcggactt catcgcacaa tgccgcgaca aacggaaaac
     7501 cgccaccttg tttgcccgct acggcatacg ctcacccgaa atttacgacc gcaagaagtt
     7561 ggttttccct tgcttcgcca agccttatga cggcagccgc gccatcggtg caaaacggat
     7621 tgacacacct gccgacctga caccggaagt cttggcagac accaaattaa tgttctgtca
     7681 gtttatcgac atcgaaaata aattttccga gtttaccgtc gatatgtatt acgaccgtca
     7741 atgccgtctg aaatgcgcca tcccgcgaaa acgcttggaa gtgcgctccg gcgaaatcag
     7801 caaaggcgtt acctgcaaaa acagcctcta ccaaacgctg ctggaaaaaa tggcggtatt
     7861 gcagggcgcg cgcggctgta tcacggcgca gttcttctac aacgaagaaa ccggcgaatt
     7921 ctacggcgtg gaaatcaacc cccgcttcgg cggtggcttc ccgctgacgt atgccgcagg
     7981 cggcaattat ccgagccggt tgatgcgcga atacctcggc gacgaagaaa tcccgttttc
     8041 agacgactgg gaaaacaacc tcattatgtt gcgctacgat gccaaggtct tggttcatga
     8101 aaacgattaa tcctgaaacc accgtcatcg tcttcgactt ggacgatacc ctgtattcgg
     8161 aatacgaata caagctctcc ggtatccgtt cggtcatcga taccgttgcc gcactgtatc
     8221 ccgattggaa ttcagacaac ctatggcgca acatcgaccc cgacggcaaa gattggctgg
     8281 acaaattgtg ccgccactgt ggttttaatg aatcggaaaa acaggttctt ttgtggcaat
     8341 accggctgca ccgcccgacg ctgactccgt atgctccgcc ggatttcctg tccgaactga
     8401 ccgcgccctt cgccgcacgc gcgctgatta ccgacggaag aagcctgacc caacgcctga
     8461 agctggaagc cttgggtctg tcttccctgt ttgacgacat cctcatctct gaagcctgct
     8521 cttcggaaaa gcccgacggc aaacgcttcc gccatcttca agacaaatat gccgacaagg
     8581 caggctgctt catctatatc ggcgacaaca tttccaaaga ctttatcgcg ccgaacactt
     8641 tgggctggat aaccatagga ttgcttccct ccggacagaa catccaccgc caccagcctg
     8701 aaagttttaa cacagaatat catcccgact tttgggttaa ctcccttcaa gatttagttc
     8761 ctataattga ttcaaatact taaccattac ataatcaaac atgcactcac aatattaaaa
     8821 ggactatttc atgacaaaat attattcatg gggcgtaaac ggctttccgc cctacgtttt
     8881 cgacaccatt tggaaacgat ggaagttgaa tgggcgcggg ggcggttatc gtgtgcgaca
     8941 tcccggacgg catgaccgtc gcgggcaatc cggcaaagcc gctgccgcgc aaaaaccccg
     9001 agaccgcgac ggcataagcg attaaaatac acccccgtac agaccgattt tggcaacacc
     9061 tgcggcgcgc gcccgattct tcggaacacg cccccttcag acggcataga gtcagaaatg
     9121 ccgtctgaaa accgacggac caaacaccat gctgaacacc tccctttccc cgtggccctg
     9181 cttcacccaa gaagaagccg atgccgtttc caaagtcctg ctgtccaaca aagtcaacta
     9241 ctggacggga aacgaatgcc gcaaatttga aaaagaattc gccgcctttg ccggcacgcg
     9301 gtacgccgtc gccctttcca acggcacgct ggcactcgat gtcgcgctca aagcaatggg
     9361 cataggcgcg ggcgacgatg tgattgttac ctcgcgcact ttcctggctt ccgcatcctg
     9421 cattgtgaac gcgggcgcaa atccggtgtt tgccgatgtg gatttgaaca gccaaaacat
     9481 cagcgcggaa accgtcaaag ccgtgctgac cccgaatacc aaggcgatta tcgtggttca
     9541 ccttgccggt atgcctgccg aaatggatgg cattatggct ttggcaaaag aacataatct
     9601 ttgggttgtc gaagactgcg cccaagcgca cggcgcgaaa tacaaaggca aatccgtcgg
     9661 cagcatcgga cacgtcggcg cgtggtcgtt ctgccaagac aaaatcatga ccacgggcgg
     9721 cgaaggcggc atggttacca ccaacgacaa agaactgtgg agcaaaatgt ggtcgtacaa
     9781 agaccacggc aaaagctatg acgccgtgta ccaccgcgaa cacgcccctg gcttccgctg
     9841 gctgcacgaa agcttcggca caaactggcg tatgatggaa atgcaggcgg caatcgggcg
     9901 cattcagctc aaacgcctgc ccgaatggac tgtctgccgt caagcgcacg ccgccaagct
     9961 ggcggaaagc ctaggcaaat tcaaaagtat ccgcttggtt gaagtcgccg actacatcgg
    10021 acacgcgcaa tataagttct acgccttcgt caaacccgaa cacctcaaag acggctggac
    10081 gcgcgaccgc atcgtcaacg aactgaacgc gcgcaaagtc ccctgctatc aaggtagctg
    10141 ctccgaagtg tatttggaga aatccttcga caacacgcct tggcgtccga aagagcggct
    10201 gaaaaatgct gtcgagttgg gcgacaccag cctgatgttc ttagtacatc cgacgctgac
    10261 cgacggcgaa atcgcgttct gcaaagaaca catcgaagcc gtattgaccg aagccacacg
    10321 ataacccttc agacggcata tgccgcctga aagcacatac cgccccacga tatgactctg
    10381 gaaaccctgc ttgccctacc gcgcaacatc aagaaaatct gtttcctcat acacgatttt
    10441 ctgatgattt tcattgcctt ttggtttacc caaagcctga aggccgacta ctcgaacgaa
    10501 tggttcgacc ctgccaactg gcagtctttc ctattgaccg cgccgttgac catcggcctg
    10561 ttcgtcagaa tggggctcta ccgtgccgtt acacgcttca tcagcttccg catcctctcc
    10621 accgcgctgg caggcagcct cgcctccgcc gtattgttct tcctcaacac attgattttt
    10681 gaagaaaggc tgcgcctcgc cctgccgatt gtctatttct tactgctgtt tgtttccgtg
    10741 accggctcgc gtatggtttt gcgcggactg ttgtccgaac accccaaaaa acagatgatc
    10801 cccgtcatca tttacggcgc ggggcaggcg ggcagacagc ttctcgaggc cgtcaaacaa
    10861 atgcgcgaat attccgccgc cgcctttgtg gacgacgacc ccaaactgtg gcacaccgtc
    10921 atctacgacc ttgccgttta ccagcccgat gccatcgcct tcctcatcga acgctacggc
    10981 gtggaaaaaa tcctactcgc catccccggc gcgacccagg aacaacgccg ccgaatcatc
    11041 aacaaactgg aagcctatcc gtgcgaagtg ttgaccattc ccggaatgaa agacctgatg
    11101 gacgggaaaa tcagcatcgg cacgctcaaa aaaatctctg tgtccgacct gctcgggcgt
    11161 gattccgtcg cgcccgacaa ccgcctgatg agtgccgaca tcgaaggcaa aaccgtcatg
    11221 gtaaccggcg cgggcggctc catcggttcg gaactctgcc gccagattat ccgccgccgc
    11281 cccgaaaagc tgctgctgtt cgagttatcc gaattcgccc tgtacgccgt cgaaaaagaa
    11341 ttgcgcgaaa cctgcatcca aaaacgcctc gacaccgaaa tcctgccctt tctcggttcg
    11401 gtgcaaaacc gcacgctgct cgaacacgtc atgaccgcct tttccgttgc gaccgtctat
    11461 cacgccgccg cctacaaaca cgtccccatg gtcgagttca acaccgtcga aggcatacgc
    11521 aacaacatct tcggcacact cgagtgcgcg cttgccgcca cgacatcggg cgtggaaact
    11581 ttcgtcctca tctccaccga caaagccgtc cgccccacca acaccatggg tgccagcaaa
    11641 cgcatggcgg aactctgcct tcaggcgctc gccgccgaac ccggacaaaa aacccgcttc
    11701 agcatggtgc gtttcggcaa tgttttaggt tcgtccggct ccgtcgtccc cgcatttgaa
    11761 aaacaaattg ccgaaggcgg cccgattacc ctgacccacc aagacattac ccgctacttc
    11821 atgaccattc ccgaagcggc gcaactggtt atccaagccg gcgcgatggg caaaggcggc
    11881 gacgtattcg tcctcgatat gggcgaatcc gtcaaaatca tcgaccttgc ccgccaaatg
    11941 attaccttaa gcggcctcaa acccaaaaca cccgaacaac ccgacggcga catcgaaatc
    12001 ctcattaccg gactgcgtcc cggcgaaaaa ctctacgaag agctgctcat cggcgacaac
    12061 gtccgcaaaa ccggccatcc gcgcattatg acggctgacg aggcgatgct gccgtggcac
    12121 gagctctctg tcctgctcga ccgcatccgt gcggcctgcg accgttacga ccagcaggca
    12181 atccgcaccc tgctcatcaa cgccccgacc ggctttaccc caagcgacgg tatctgcgac
    12241 ctgctttggg tacgagaaac acacagaaaa aatgccgtct gaaccttcag acggcataac
    12301 gtacaaacca acctacctta cacacacgga gtttgacatg cagttctcag cattcggcga
    12361 aaaattcacg caacacagcg gcatcctcca actgatggac gacctcggcg acgcgctcaa
    12421 aagcgacaag cccgtcaata tgctcggcgg cggcaatccg gcgcgaatcg ccgaagtcaa
    12481 tgccgtgttc gccgacgtat tttccaaact ggcagcagaa catgccgtcg aaaacatcgg
    12541 caactactcc aatcctcaag gagatgccgc gctgattgac gcgctgaccg ccttcctcaa
    12601 ccgcgaatac ggctggaacc tgaccgccga caatatcgcg ctgaccaacg gttcgcaaaa
    12661 cgcgtttttc tatttattca acctcttcgg cggcaaattc aacctttcag acggcacatc
    12721 cgcagaaaaa gccattttgt tgccgctcgc gcccgaatac atcggctatg ccgacgtgca
    12781 tgtcgaaggg cagcacttcg tttccgtcaa gcccaaaatc gaaaacgtcg aacacgaagg
    12841 cgaagcaggc ttcttcaaat accgcgtgga ctttgacgca ctggaaaacc tgcccgaact
    12901 caaagcgggc aaaatcggcg cgatttgctg ttcgcgcccg accaacccga ccggcaacgt
    12961 gctgaccgac agcgaaatgg cgcggctgga cgctttggcg caagaacacg gcattccgct
    13021 gattatcgac aacgcctacg gaatgccgtt ccccaacatc atttacagcg acgtaacgct
    13081 gaattggcat gaaaacatca tcctctgctt cagcctttcc aaagtcggtc tgccgggcgt
    13141 gcgcaccggc atcatcgtcg ccgcgcccga agtcgtcaaa gccgtcagca gcctgaacgc
    13201 gattgtgaac cttgccccca cgcgcttcgg cgcggccatc gccgccccgc tactggaaag
    13261 cggcgagatg aaacggcttg ccgaccaagt catccggccg ttttaccgca atcaggcgca
    13321 aaccgccgtc tcgctgctca agcgcgagct gggcgcgtac ccgatgaaaa tccacaaacc
    13381 cgaaggcgcg attttcctgt ggctctggtt tgaaaacctg cccgtttctt cgcaaaccct
    13441 gtacgaaatg ctcaaagccg aaggtacgct gattattccg ggcgaacatt tcttcgtcgg
    13501 catcgacacg cgggattatc cgcacgcggg cgagtgcatc cgtatgagca tcgcgcagga
    13561 cgctcaaacg ctggaaaaag gcattgccgc catcggtaaa accgtccgaa aactgtacga
    13621 caacgtttaa accgcaaaaa atgccgtctg aaggcctttc agacggcatt tttatccgca
    13681 ttcaatgtcg ggaaaaatgc gcccacactg gcctgcagtt ttccggcagt tcgggacacg
    13741 cgccgaggat gccgccgctg aagtcgttta aacggtggaa gtcgctgccc gcactggcga
    13801 gcatgccgaa gcgttctgcc aaaaatgcgt agttgaggcg gtcgtttttg cagcagttgc
    13861 cgctgtggac ttcgatgccc gcgccgccga ggtttttaaa ttcttcaaac agattgcgct
    13921 tggcggtagc ggacaaatca tagcgcatcg gatgggcgat gactgccatg ccgcccgcgc
    13981 cgttgacggc ggagacgcag tcttccagcg tcgcccattc gtgtcggacg gcgcaggatt
    14041 tgccgtcgcc caagtatttg gtgaacgcct gctgcttgtt tttgacgtgt cccacccgaa
    14101 tgaggaactc ggcgacgtgt gtgcggctga ccatttcttt gtttgccgcc agcgcgagcg
    14161 cgccgtcgta tgcgcccgaa atgccgcgtt tttccagctt ggcggcgatg gcttcgaggc
    14221 gtttcagacg gccttgccgc acttgcgcca acaggttttg caggttttcg tcttgttcgt
    14281 caaagtctaa gccgacaacg tgtatggtac gcccgcgcca cgttacggag atttccacgc
    14341 cgttaatcag gcgcaaaccg agcctgtcgg cttcggcacg cgcttcggcg atgccgccgg
    14401 tgtggtcgtg gtcggtcaac gccagcagcg tgcagccgtt ttgatgcgcg aggcgcacaa
    14461 cttcggcggg ggagagcata ccgtcggaaa cggtggaatg gcagtgcagg tctatcataa
    14521 ggcgttatgt gcgtggtaaa tggtgggcag ggtgtttcaa tattgtcggg cggtgtggcg
    14581 gcggtgattt caaaaaacag gtgtatggca aatgcaaagg aaaagccgct atgccgtcat
    14641 tccagcgcag gcgggaatcc agactttgat ttattggaaa tgtttaaggt taaccgctat
    14701 ttcaaacttc cggattcctg ctttcgaggg aatggcgata tgaccgtttt caattttaat
    14761 ctactataaa agactgtctg aaaacgtggt tttatagtga attaaattta aaccggtaca
    14821 gcgttggctc gccttgccgt actatttgta ctgtctgcgg ctcgccgcct tgtcctgatt
    14881 tttgttaatt cactatatca agccgaaccg ttttcagacg gcatcgtccg accaacccgc
    14941 ttctttcaat ttccgccgtt gcgcgtcgta gcgggcctta tccgcccaat aaatcgtctg
    15001 aatacacgcc tcgccgcagt cgctgccgca gcattcccac gactcgggtc ggacgggttc
    15061 gtctaaaagc ggctcgccca aaagggcttc ggctttatgc ttcagggtca tatccatcgg
    15121 cgatttccaa gcgcgcaccg tcaaactcga tgacttcgcc gccgcgtatt ttggcggttt
    15181 tacgggtttc ggtttcgccg ttgcgcgaca ccaacccttc ggcaatcaac gccttcgcct
    15241 gtccgccgct ttcggcaagt ccggccaatt tcaagaggtc gcacaaggcg atgtattcgt
    15301 tgtcttcgag atagacgatg gcttccatga tgtccccttg cagaaagaga ccgttattgt
    15361 agcacctgcc gccgccgtac ccaaaattac cgcaaaacca gcgatgtatc cgcaccgcct
    15421 gttccgtaaa agtaaaaatg ccgtctgaaa ccccgtatgc cgcaatccgt tcaaagaaat
    15481 cctgcccaac ggcagactgc aaatcctgtt ccccgacgaa tccgcattga cgctgatgca
    15541 catcctcaaa cacgaactgc ccgatacacc ggcaatcggc atcaaaacga aatcaaaaac
    15601 ctgtttgaac gtaaaattaa tttaactgct tgccatccgt ccgaataagg tatatagttc
    15661 cttataacta gtttgatagt cctttatatc tatcaatact ccttgggaag cctccaccac
    15721 acggtaggag gcattttttt gccgtcgtaa aagctcaaaa acatttgcag gtcatgccgt
    15781 ctgaatctga aacggcatga cctacaccgc catctaaaga caaccctgct acaatacgcc
    15841 ttttattgtt cacgccgatt ttgccatgac cgagccgacc tacatccccc tgcgcctgca
    15901 taccgagttc tcaattaccg acggtatggt gcggattaaa aaactgattg ccaaagcgca
    15961 ggaatacggt ttgcctgctt tgggcatcag cgatttgatg aacgaattcg gtttggtgaa
    16021 attctataaa gcctgccgca gtgcggggat taagcctgtc ggggcggcgg acgtgtggat
    16081 aggcaatccg aatgcgcccg acaagccgtt ccgcgccatg ttgattatcc gcaacgatgc
    16141 gggctatcag cgtttgagtg agcttttgac agaggcctat gtcggtaaag accgcaatgt
    16201 ccatcatgcg gaactcaacc ccgaatggct ggaaaacggc gacaacagcg gcttgatttg
    16261 tttgagcggc gcgcattacg gcgaagtggg catgaatctg ttgaacggca atgaagacgc
    16321 ggcgcgcgcg gcggcgttga aatatgcggc atggtttccc gatgcgtttt atttagagct
    16381 gcaacgcctg cccgaacgcc ccgaatggga ggcttgcgtt tccggcagcg tgaagctggc
    16441 ggaggaactg ggtttgccgg tggtggcgac gcatccgacg cagtttatga accgcgacga
    16501 tttcaacgcg cacgaggcgc gggtgtgtat cgcgggcggc tgggtattga cggacaaaaa
    16561 acgtccgcgc gatttcacgc cggaccagtt tttcattccg ccggaaacga tgctggagcg
    16621 tttcgccgac ttgcccgaag ccttggaaaa cacggtggaa atcgccaaac gctgcaacct
    16681 gcacatcaca ttgggcaaaa acttcctgcc ccttttcccc acgcccgacg gtttatcgcg
    16741 cgatgactat ctcatcaaac tctcaaacaa gggcttgcag gaacgcatgg ttcagcttta
    16801 tcccgacgag gcggagcgtg cggcaaaaat gccggaatat caggaacgtt tggattttga
    16861 gctgaacatc atcatccaga tgaaattccc cggctatttc cttatcgtac aagactttat
    16921 caactgggcg aaaacgcacg gctgtccggt cgggccggga cgcggttcgg gcgcgggttc
    16981 gctggtggcg tattcgctga aaattaccga cctcgacccg ctcaaatacg cgctgctgtt
    17041 cgaacgtttc ttgaaccctg aacgcgtttc catgcccgac ttcgacgtgg acttctgtca
    17101 ggcaaaccgc ggccgcgtga ttgaatatgt acgcgaaaaa tacggtgcgg aagcagtcag
    17161 ccagattgtt accttcggca cgatgtcgtc caaagcagtg attcgcgacg tggggcgcgt
    17221 actggaactg ccgtttatgc tgtgcgacaa actgtccaag ctgattccgt tggaagccaa
    17281 caaacctttg ggtttggacg acgcgatgaa ggcggagccg cagattcagg aattaatcga
    17341 agcggaagaa gcggacgaac tgattacgct ggcgaaaaag ctggaagatt tgacgcgcgg
    17401 tttgggtatg cacgcgggcg gcgtgttgat tgcgccgggc aagatttccg attacagccc
    17461 cgtgtatcag gcggacgaat ccgcctcgcc cgtatccatg tacgacaagg gcgacgtgga
    17521 agacgtgggt ttggtgaaat tcgacttttt aggtctgcgc aacctgacca tcatcgaaat
    17581 ggcgcagaac aacatcaaaa acaccaccgg cgacatcatc gatgtcggca aaattccgct
    17641 tgacgaccag gttgcctacc aaatcttccg cgatgcgaac accaccgccg tgttccagtt
    17701 cgagtcgaca ggcatgaaga aaatgctgaa aacagcgcat acgaccaagt ttgaagaact
    17761 tatcgccttc gtatcgctct accgacccgg cccgatggac aatattcccg acttcgtcgc
    17821 gcgtatgaaa ggtcaagaat tccaatacat ccacccgcta ctcgaaggca tcctcgcacc
    17881 aacctacggg attatggtgt atcaggaaca agtgatgcag gcggcgcaga ttatcggcgg
    17941 ctactcgctc ggcggcgcgg acctgctgcg ccgcgcgatg ggtaagaaaa agcccgaaga
    18001 aatggtgaaa caccgcgaaa tcttcgccga aggcgcggca aaacaaggca tttcgcgcga
    18061 aaaatccgac gaaatcttca actatatgga aaaattcgcc ggctacggtt tcaacaaatc
    18121 ccacgccgcc gcctacgccc tgatttccta ccagaccgca tggctcaagg cgcactaccc
    18181 cgccgaattt atggcggcga ccatgtcgtc cgaattggac aacaccgacc agctcaagca
    18241 tttctacgac gactgccgcg ccaacggcat cgagttcctg ccgcccgaca tcaacgaatc
    18301 cgactaccgc tttacgccgt atccggacat gaaaatccgc tacgcgctcg gcgcgattaa
    18361 aggcacgggc gaagccgccg tcgaatccat caccgccgcg cggcaaagcg gcggcaagtt
    18421 taccggcctg ttggacttct gcgagcgcgt cggcaaagaa cacatgaacc gccgcaccct
    18481 cgaagccctg atacgcggcg gcgcgttcga cagtatcgaa cccaaccgcg ccatgctctt
    18541 ggcgaacatc gacctcgcta tggacaacgc cgaccaaaaa gccgccaacg ccaatcaggg
    18601 cgggctgttc gacatgatgg aagacgccat cgaaccggtg cggctcatcg acctgccgag
    18661 gtggagcgaa tcggaaaaac tcgccgaaga aaaaaccgtc atcggctttt acctgtccgg
    18721 ccacccgttc ggcccgtatg cccaagaagt ctgccaaatc gcaccgacca aattagaccg
    18781 tctgaagccg caagacagcg tgcgcctcgc cggattcgtt accgccgtgc gtacgatgat
    18841 gggcaagcgc ggcaaaatcg ccttcgtcag ccttgaagat ttgagcgggc aggttgaaat
    18901 catggtcggc agtcagacgc tggaaaactg cgccgactgc ctcaaagccg accaagtgct
    18961 gattatcgaa tccaaagtca gccgcgacga ctacggcggc ggcgacggac tgcgcatcat
    19021 ggcaaaccaa gtcatgaccc tgcaaacggc acgcgaacgc tacgcccgca gcctcagcct
    19081 cgccctcgcc ccgcatcacg acatcggcgg actggtacgg ctgctctcct cccaccaact
    19141 gcccgacacg ccgcgcatcc cgctgcaact gtcgtatgcc aacgaaaaag cgtcgggcag
    19201 gcttcaagtg ccgccgaaat ggacggttac accgagctcc gcattgttcg gcgaactgga
    19261 aacattgctc ggcagccggt cggtgcgcgt caactggtaa cccaaaatat aaatgccgtc
    19321 tgaagcccaa aatcggtttc agacggcatt ttttaaccga aataaacgat gccccttaaa
    19381 gcagaagctt taaggggcag agcgttgcgg cacatctttt cagacggcct tattgtagca
    19441 acggttctca tttcgcagtg cgacaatatg tttgggattc tagccgttgt gagatgataa
    19501 gtcaataatc tatttgtata taaataagac aataagatat attatcatta tttgcaacgt
    19561 tgtttacgga gtaataaatg gctgccttca aacctaatcc aatcaactac atcctcggcc
    19621 tcgatatcgg catcgcatcc gtcggctggg cgatggtaga aattgacgaa gaagaaaacc
    19681 ccatccgcct gattgatttg ggcgtgcgcg tatttgagcg tgccgaagta ccgaaaacag
    19741 gcgactccct tgccatggca aggcgtttgg cgcgcagtgt ccgccgcctg acccgccgtc
    19801 gcgcccatcg cctgcttcgg gcccgccgcc tattgaaacg cgaaggcgta ttacaagccg
    19861 ctgattttga cgaaaacggc ttgatcaaat ccttaccgaa tacaccatgg caacttcgcg
    19921 cagccgcatt agaccgcaaa ctgacgcctt tagagtggtc ggcagtcttg ttgcatttaa
    19981 tcaaacaccg cggttatttg tcgcaaagaa aaaacgaggg cgaaactgcc gataaagagc
    20041 ttggcgcttt gcttaaaggc gtggccaaca atgcccatgc cttacagaca ggcgatttcc
    20101 gcacaccggc cgaattggct ttaaataaat ttgagaaaga aagcggccat atccgcaatc
    20161 agcgcggcga ttattcgcat acgttcagcc gcaaagattt acaggcggag ctgattttgc
    20221 tgtttgaaaa acaaaaagaa tttggcaatc cgcatgtttc aggcggcctt aaagaaggta
    20281 ttgaaaccct actgatgacg caacgccctg ccctgtccgg cgatgccgtt caaaaaatgt
    20341 tggggcattg caccttcgaa ccggcagagc cgaaagccgc taaaaacacc tacacagccg
    20401 aacgtttcat ctggctgacc aagctgaaca acctgcgtat tttagagcaa ggcagcgagc
    20461 ggccattgac cgataccgaa cgcgccacgc ttatggacga gccatacaga aaatccaaac
    20521 tgacttacgc acaagcccgt aagctgctgg gtttagaaga taccgccttt ttcaaaggct
    20581 tgcgctatgg taaagacaat gccgaagcct caacattgat ggaaatgaag gcctaccatg
    20641 ccatcagccg tgcactggaa aaagaaggat tgaaagataa aaaatcccca ttaaaccttt
    20701 cttccgaatt acaagatgaa atcggcacgg cattctccct gttcaaaacc gatgaagaca
    20761 ttacaggccg tctgaaagac cgtgttcagc ccgaaatctt agaagcgctg ttgaaacaca
    20821 tcagcttcga taagttcgtc caaatttcct tgaaagcatt gcgccgaatt gtgcctctaa
    20881 tggagcaagg caaacgttac gatgaagcct gcgccgaaat ctacggagac cattacggca
    20941 agaaaaatac ggaagaaaaa atttatctgc cgcccatccc tgccgacaag atccgcaacc
    21001 ccgtcgtctt gcgcgcctta tctcaagcac gtaaggtcat taacggcgtg gtacgccgtt
    21061 acggctcccc agctcgtatc catattgaaa cggcaaggga agtaggtaaa tcgtttaaag
    21121 accgcaaaga aatcgaaaaa cgccaagaag aaaaccgcaa agaccgggaa aaagccgccg
    21181 ccaaattccg agagtatttc cccaattttg tcggcgaacc caaatcaaaa gatattctga
    21241 aactgcgcct gtacgagcaa caacacggca aatgcctgta ttcgggcaaa gaaatcaact
    21301 tagtccgtct gaacgaaaaa ggctatgtcg aaatcgacca tgccctgccg ttttcgcgca
    21361 catgggacga cagtttcaac aataaagtgc tggtattggg cagcgaaaac caaaacaaag
    21421 gcaatcaaac cccttacgaa tacttcaacg gcaaagacaa cagccgcgaa tggcaggaat
    21481 ttaaagcgcg tgtcgaaacc agccgtttcc cgcgcagtaa aaaacaacgg attctgctgc
    21541 aaaaattcga tgaagacggc tttaaagaat gcaatctgaa cgacacgcgc tacgtcaacc
    21601 gtttcctgtg ccaatttgtt gccgaccata tattgctgac aggtaaaggg aaaagacgtg
    21661 tctttgcctc aaacggacaa attaccaatc tgttgcgcgg cttttgggga ttgcgcaaag
    21721 tgcgtgcgga aaacgaccgc catcacgcct tggacgctgt agtcgttgcc tgctcgaccg
    21781 ttgccatgca gcagaaaatt acccgttttg tacgctataa agagatgaac gcgtttgacg
    21841 gtaaaaccat agacaaagaa acaggaaaag tgctgcatca aaaaacacac ttcccacaac
    21901 cttgggaatt tttcgcacaa gaagtcatga ttcgcgtctt cggcaaaccg gacggcaaac
    21961 ccgaattcga agaagccgat accccagaaa aactgcgcac gttgcttgcc gaaaaattat
    22021 catctcgccc cgaagccgta cacgaatacg ttacgccact gtttgtttca cgcgcgccca
    22081 atcggaagat gagcggtgca cataaagata ctttgagatc tgctaaacga tttgttaaac
    22141 ataatgaaaa aattagtgtt aaacgagtat ggttaaccga aatcaagttg gccgaccttg
    22201 aaaatatggt taattataaa aatggtagag agattgaatt atatgaggct cttaaggcgc
    22261 gtttagaggc atatggaggt aatgctaaac aagcatttga ccctaaggac aatccgtttt
    22321 ataaaaaggg aggacaactg gttaaagctg tgagggtcga aaaaacccaa gagagcggag
    22381 tcttattaaa taaaaaaaat gcttatacca ttgcagataa tggagacatg gtacgtgttg
    22441 atgtgttctg taaagtagat aagaaaggaa aaaatcagta ttttattgtt cccatctatg
    22501 cttggcaggt tgctgaaaac attcttcccg atattgattg taagggatac cggattgatg
    22561 atagctatac attctgtttt agcttgcata agtatgatct gattgctttt caaaaagatg
    22621 aaaaatctaa agtagaattc gcctactata tcaactgtga tagctctaat ggacgattct
    22681 atttagcttg gcatgataaa ggctctaaag aacagcaatt ccgtattagc acccaaaatc
    22741 ttgtattgat acaaaaatac caagttaacg aactgggcaa agaaatcaga ccatgccgtc
    22801 tgaaaaaacg cccacctgtc cgttaaatta atctgtccct gcttcagacg gcctgaagca
    22861 gggatttttt atatcaaaat aaggggaaaa catgacttgg cgcagcctac ttattcaaaa
    22921 cggcggcaaa ctatccttgc aacgccggca actgctgatt caacaaaacg gcgagtccca
    22981 taccgtacca ttagaagaca tcgccgttat catcatcgaa aatcgcgaaa ccctgattac
    23041 cgccccgctc ctatcagcct tagctgaaca tggcgcaacc ctgctgacct gcgacgagca
    23101 gttcctgccc tgcggacaat ggctgcctta tgcccaatat caccgccagc ttaaaatcct
    23161 caagctgcaa ctgaacataa gcgaaccctt aaaaaagcaa ttctggcaac atatcgtccg
    23221 ccaaaaaatc ctgaaccaag catttatcgc agacgaaaca ggcaatgatc ttgcagccaa
    23281 acggctgcgc actttggcat ctgaagtccg ttcgggcgat acaggaaacc gtgaagccca
    23341 agctgccgcc ctctacttcc aagccctctt tggcgaaaaa tttacccgta atgacaacaa
    23401 tgccgtcaat gccgcattga actacaccta cgccgtgcta cgggccgcag ttgcacgcac
    23461 cctgacctta tacggctggc tgcctgcatt aggcttgttt catagaagcg aactcaatcc
    23521 attcaatctc gccgatgact ttatcgaacc cttacgccca ttagccgacc tgaccgtgat
    23581 acatttgtac gaacaaggcc gtctgaaagc cgaactgacc cccggcatca aacaacacct
    23641 tatcaaaacc cttcactatc aaatcagcat cgaacggcaa catttcagca ccttggctgc
    23701 catcgacaaa atggtttcgt catttcaagc cggtgtaacc gataaaaacg ccaaacaact
    23761 gaaactgcct gaaatcctac cattgaaaga ataccgatat gagtgaggcc aaatttatga
    23821 ggattatcgt cttcttcgac ctaccggtta tcacggcggc aaagcgcaaa gccgccaatc
    23881 aattccgcca gtttttatta aaagacggat accaaatgct ccagctttcc gtatacagcc
    23941 gtatcgtcaa aggccgcgat tcgttgcaaa aacaccacaa tagactatgt gcaaaccttc
    24001 cgcaagaagg ctcaatccgc tgtttggaga taacagaaaa gcaatatgcc gccatgaaac
    24061 tgctgttggg cgagctgaaa acccaagaaa aaaaggtcaa ttcagaccaa ttattgttat
    24121 tttaagccca ttttttcaga ctaaataaaa cgggaaatcc ttatagaata aggatttccc
    24181 gttggggtat tgtagcactg cgaaatgaga atgggagcta caacaaaagt ttatgacttg
    24241 tacgaatccg cagaattgta gcactgcgaa atgagaatgg gagctacaac ccctgattgc
    24301 aggtgaagat gaactgtatg attgtagcac tgcgaaatga gaatgggagc tacaacaaaa
    24361 tcgtcggcct gctccagcac gacctgattg tagcactgcg aaatgagaat gggagctaca
    24421 acctacccgc tcggcgtata tgaggcaatc taattgtagc actgcgaaat gagaatggga
    24481 gctacaacgc gcacgattga cggcagcaga cgagccaaat tgtagcactg cgaaatgaga
    24541 atgggagcta caacagcggt ggtagaatcg gatttttaga gtgaattgta gcactgcgaa
    24601 atgagaatgg gagctacaac cgtttttcag gtcgaattat cgaaccttca attgtagcac
    24661 tgcgaaatga gaatgggagc tacaacctcg aatggcgagt acttgacgct caatacattg
    24721 tagcactgcg aaatgagaat gggagctaca accaggtgct tgggcgtaat tttatccgtt
    24781 ggattgtagc actgcgaaat gagaatggga gctacaaccc ttttgcaaca atcgtgcttt
    24841 atttgtcaat tgtagcactg cgaaatgaga atgggagcta caacttcctg tcggcttttt
    24901 cggctgcttt gcggattgta gcactgcgaa atgagaatgg gagctacaac tatgcttggg
    24961 tttgagtggg cgattgatga attgtagcac tgcgaaatga gaatgggagc tacaaccttg
    25021 gcacgagatg gtcgccgtat taaattattg tagcactgcg aaatgagaat gggagctaca
    25081 actttatggc tattcacctg tcgagcaaat caattgtagc actgcgaaat gagaatggga
    25141 gctacaacaa gaagattggg aaatgtatct cacggaagat tgtagcactg cgaaatgaga
    25201 atgggagcta caaccggttt gaacagcagt atatgggaag aaacattgta gcactgcgaa
    25261 atgagaatgg gcgctacaac cagccgttgc gataagcgaa cgtgcgttaa agtcattgag
    25321 acacagtgca attatggcaa gaaaggcgac agatattgtg tcacagattt gaaataagaa
    25381 aaaaccgccc actcacagag taggcggaaa acttgcgttt ttttgattgt ggtcgggtta
    25441 gctactgcat tgacttaaca gcaaagcaac cgctaaaatg aacttgtggt tcacttcaac
    25501 ggacagcagc aactttttcc cacgcataag gaatttaagc attttagctt ctcctagtga
    25561 agttaatcac acaggcgaga gccgaacaaa aatccccgtt gcagcgggga tttttgctgt
    25621 tccctgtttg atgattaaac taaaatcttg caaa
//
LOCUS       DAWHXZ010000004        23412 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000004 DAWHXZ010000000
VERSION     DAWHXZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23412
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.004"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(339..761)
                     /locus_tag="WIE35_000109"
     CDS             complement(339..761)
                     /locus_tag="WIE35_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ4153100.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(879..1550)
                     /locus_tag="WIE35_000110"
     CDS             complement(879..1550)
                     /locus_tag="WIE35_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ4153101.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPSGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1566..2192)
                     /locus_tag="WIE35_000111"
     CDS             complement(1566..2192)
                     /locus_tag="WIE35_000111"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ4153102.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNRETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCARYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2299..3105)
                     /locus_tag="WIE35_000112"
     CDS             complement(2299..3105)
                     /locus_tag="WIE35_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ4153103.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAASEPAAASAAQGDTSSIG
                     STMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMM
                     KFLQEQQAKAVEKHKAEAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQP
                     TKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYI
                     PSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3364..3804)
                     /locus_tag="WIE35_000113"
     CDS             complement(3364..3804)
                     /locus_tag="WIE35_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ4153104.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3867..5273)
                     /locus_tag="WIE35_000114"
     CDS             complement(3867..5273)
                     /locus_tag="WIE35_000114"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ4153105.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5443..6558
                     /gene="lptF"
                     /locus_tag="WIE35_000115"
     CDS             5443..6558
                     /gene="lptF"
                     /locus_tag="WIE35_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ4153106.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6555..7625
                     /gene="lptG"
                     /locus_tag="WIE35_000116"
     CDS             6555..7625
                     /gene="lptG"
                     /locus_tag="WIE35_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ4153107.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7935..10520
                     /gene="acnB"
                     /locus_tag="WIE35_000117"
     CDS             7935..10520
                     /gene="acnB"
                     /locus_tag="WIE35_000117"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ4153108.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10662..11657
                     /locus_tag="WIE35_000118"
     CDS             10662..11657
                     /locus_tag="WIE35_000118"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ4153109.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEIFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(11726..11827)
                     /locus_tag="WIE35_000119"
                     /pseudo
     CDS             complement(11726..11827)
                     /locus_tag="WIE35_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11881..12894)
                     /gene="ilvC"
                     /locus_tag="WIE35_000120"
     CDS             complement(11881..12894)
                     /gene="ilvC"
                     /locus_tag="WIE35_000120"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ4153110.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(12974..13267)
                     /locus_tag="WIE35_000121"
     CDS             complement(12974..13267)
                     /locus_tag="WIE35_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ4153111.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            complement(13334..13825)
                     /gene="ilvN"
                     /locus_tag="WIE35_000122"
     CDS             complement(13334..13825)
                     /gene="ilvN"
                     /locus_tag="WIE35_000122"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ4153112.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(13836..15563)
                     /gene="ilvB"
                     /locus_tag="WIE35_000123"
     CDS             complement(13836..15563)
                     /gene="ilvB"
                     /locus_tag="WIE35_000123"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ4153113.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSSDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDTLGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
     gene            complement(16272..16356)
                     /locus_tag="WIE35_000124"
     tRNA            complement(16272..16356)
                     /locus_tag="WIE35_000124"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16320..16322),aa:Leu,seq:caa)
     gene            16632..17285
                     /locus_tag="WIE35_000125"
     CDS             16632..17285
                     /locus_tag="WIE35_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ4153114.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            17364..18017
                     /locus_tag="WIE35_000126"
     CDS             17364..18017
                     /locus_tag="WIE35_000126"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ4153115.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            18121..19020
                     /locus_tag="WIE35_000127"
     CDS             18121..19020
                     /locus_tag="WIE35_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ4153116.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            19017..20306
                     /gene="hisD"
                     /locus_tag="WIE35_000128"
     CDS             19017..20306
                     /gene="hisD"
                     /locus_tag="WIE35_000128"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ4153117.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            20352..21449
                     /locus_tag="WIE35_000129"
     CDS             20352..21449
                     /locus_tag="WIE35_000129"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ4153118.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            21446..22363
                     /gene="hisB"
                     /locus_tag="WIE35_000130"
     CDS             21446..22363
                     /gene="hisB"
                     /locus_tag="WIE35_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ4153119.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPNARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            22458..23327
                     /locus_tag="WIE35_000131"
     CDS             22458..23327
                     /locus_tag="WIE35_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ4153120.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQVGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT         5697 a         6505 c         5824 g         5386 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactataatt caataaatta atatatggct taaaataacg
       61 ggattctcgc ctcccgcccg cccgcagaag caggcgggta tcattttaaa acgcggcatt
      121 taaaatttga ccgaaaattg ttgacaatcc ggaatcaagt ctgcacaata ccccgacaag
      181 tccaagtatt ataaaggctg aataaagagg aaacagcagg cagatatatt cgggaggtgc
      241 agtccgaata tatctgcttt tttatgcgcc tccggattgc ctgccgcacc tttcccttca
      301 gacggcatca gccgtttccc cataatgccg cccgatgcct atttatctgc cccggcaatt
      361 tcaaaactgt gggtaatctt tgccgctttg cccaacataa tcgaagccga acagtatttt
      421 tcggcagaca tctgaacggc gcgctcaatg gccgattctt tcaaatcatg cccgaatact
      481 ttgaaatgga tgtggatttc ggtaaacacg cgcggcgcat cgtccgcccg tttcgccgta
      541 accgtcgcac ggcagtcagt cactttctga cgctgttttt cggcaatcat caccacatcg
      601 atgctcgaac agcccgccac gcccaacagc agcatttcca aagggctggg cccgcgcttg
      661 gccgcacctt ctgccgccga accctccata acgacgctgt gcccgccttc cgtcgtgccg
      721 acaaagcaca ttccgtctat ccattttgat gtaacctgca tggtgtcatt cctgaaaata
      781 gcgttaaaac cgctttgcat atggcgttat tgtaaacaat ttcaagcggc ttatgcagaa
      841 atatggacaa aacggcaaaa aaacacttga aaaccgattt acggtttggc ggactggccg
      901 ttgatctgca ccgatttgag tttcagcgta taggttttgc cgtcgtcggt atagccgatt
      961 tgtgccggaa tattgttcag ggacggtgcg aagaaataca ttaccgtatc gtcgccgcgc
     1021 cgcacccgat atttgacgac ttcggtttcc acgccgccta tgctgtattt tcctgtaccc
     1081 gccttattca aaccgccgac ggaataaagt tttttgccgt tggtgatttt cagccccgag
     1141 gggagtttcg cgtcatttgc cgccaactgc caggcaagcg tgaacaaatc catagccttg
     1201 gggctttgct cggttttgct ctcgcccgct ttgccgtagg ttacgctgcc gtcggcgaat
     1261 ttggcttccg catacagttt gcccctgcgt atgtctctat agtaggtagg gtgcagggta
     1321 ttgccgacaa ccgtaccgcc ggactcgaaa cggatattgt atagcggcac tttaatcgtc
     1381 gaaacgattt tgtaagcatt gccgctgcgt tcaaatgtca tcgtggcggg aatgccgtag
     1441 ctgccggaat agtgcagcac ggcggattgg ggcagccctg ccgcatacgc gcacggcagg
     1501 gcggcggaca aaatggcggc ggaaaatata tttttaaaag tcttcatcat ttgctcccgc
     1561 ccggtttacg ccgtcaggaa acgggcggca tcggcgtttt ccgaatttct gacgcggttt
     1621 ccctcgataa tcaggcggcc ggcggcaaaa tcggcaacgg ctttcggata aagtttatgc
     1681 tcgacagcca aaacccgtgc ggcaatatcg tctgccgtat cgccgtcaag tatcggcaca
     1741 atcccttgcg atacaatcgg gccgcaatcc agttcggcag taacaaagtg aatcgtacag
     1801 cctgcaacgc ggcaacccgc ttccaaagcg cgttcgtgcg tatgaagtcc ggtaaacgag
     1861 ggcaggatgg acgggtgaat gttcatcagc ctgccttcgt aacgggcgca aaactcgggg
     1921 gtcagaatcc gcataaaacc tgccaaaacc accaagtcgg gttgatatgc gtcgattttt
     1981 tccatcatgg ccgtatcgaa ggcaagtcgt gattcaaagt ttttatgatt caggctatcg
     2041 gtcgggatgc cgcgttcggc cgcccattgc aaaccggcag ccgtttctct gttgctcaac
     2101 acggcggcaa tgcggacgtt gggaatggcg gcattgacga ttgcctgcat attgctgccg
     2161 cgtccggaaa tcaggatgac gatgtttttc ataatggtac gcttttgaaa gggatgccat
     2221 ctgaaccgct gtttggtggt ttcagacggc atttgccgta aaaatgcccg aaaacctgtt
     2281 tcgggcatgg attcggactt aatttacttt tttgatgtcg acttgagccg gctgcttggc
     2341 gggcgcgttt tcgggtgcgc cgattttgac cagtttcaca tcaaatacca aagtggcgtt
     2401 cgggccgatt ttgtcgcccg caccctgttc gcggtaggca aggttggacg ggatgtagaa
     2461 cgtggcttcg ccgccttctt tcagaagctg tacgccttcg gtccaacccg gaatcacttg
     2521 gctcaaaggg aaggtgaccg ggccgccgtt ggctttgctg ctgtcgaata ccgtaccgtc
     2581 aatcaggcgg ccttcgtatt ccacggtaac gatgtcgtct ttggtcggct gtttgccttc
     2641 gccctgtttg gtgattttgt attgcaggcc ggaagcagtg gtcttcacgc cgtctttggc
     2701 ggcattttct ttcagaaagg cttcgccttt ttctttattg gccttcgctt ccgccttgtg
     2761 tttttctacg gctttagcct gttgttcctg aaggaatttc atcatgactt cctgagcctg
     2821 ctcttcggtc attttgattt ctttgccgtc atacactgcc tgcatggctt cggtaaagac
     2881 tttcaaatcg atttccgcac cctgttcctt catttgcttc agggagcgtc cgatgtccac
     2941 gcccatcgca tagcttgcct gctgcatcgt gctgccgatc gaagaggtgt cgccctgcgc
     3001 ggcagaagcg gcggcaggtt cggatgcggc ttcttttttg ccacaggcgg aaagtgccaa
     3061 agcggcggaa agggtcagtg cgctgatttt gaaaatggtg ttcatgatgg atcttcgctg
     3121 tcgataaggt cggaaaaacg ggattatagc cgagtttgaa tgtttcaaca cacaggatga
     3181 cacataaagc gtcaatcgtg tgttgccctg ttttggaagg gattgaacct tccaaaataa
     3241 gttttgattc taccgccccg agggacagat gtccaagtgg cggggttcaa ccgataagga
     3301 aattttaatc aaatagaatc aagcctgttt aaattttgta aatgcggcat ttcagacggc
     3361 attttatgcc ttgccctcca tgccgtgatg ttcgatggca aaaccgcttc ggcggtaggc
     3421 ggtaaagcgt tcgcgcgcgt cggcgagatc ttccaagctg ttgccgacga tttccaaaac
     3481 gcgcgcgggc aagaccggag cggtgttcca aaaaccgtcg gacaggttca aaacagtcat
     3541 gccttcggga attcggggcg gattgccgcc gcaagcaagc aggacggatg tttcagacgg
     3601 catggcttct tccatttccc aaatttcgtg cggaatgaaa ctctcggctt cgtattgcca
     3661 aagcattttg tccaattcct gaagccgccc gaacgaatcg gaccacacca gtatcctgcc
     3721 gccgtcccga atcgcacggg caatcaggcg gcaggtgaaa atcggaactt gggcaacgtg
     3781 cgtgtaaaag gtggctttcg gcatattgtt tgagcatttg gcaggacgat gccgtctgaa
     3841 aggcttcaga cggcattgtg gcaaaattaa agattccgca gatagttcag cagcaaggga
     3901 acgggacggc cagtcgcacc tttttccgct ccggatttcc acgccgtacc cgcgatgtca
     3961 aggtgcgccc acgggtagcc ttcggtgaag taagacagga atgttgcggc ggtaatcgtg
     4021 cccgcgccgg gcgtgccgat gtttggaatg tcggcaaagt tggatttgag ttggtctttg
     4081 taggtctcaa agagcggcag ttgccatgct ttgtcgtcca cgttgtagga agcggcaagc
     4141 aggctgtcga tcaaatcctg attgttgccc atcacgccgc tgacatcgtg ccccaaggca
     4201 acaatacacg cgccggtcag ggtggcgacg tcgatgacgg ctttgggttt gaactgctcg
     4261 gcgtaagtga gcgcgtcgca caaaatcaga cggccttcgg catcggtgtt caacacttcg
     4321 atggtcagac ctttcatact tttcacgaca tcgcccggtt tgtttgccgc gccggaaggc
     4381 atattttcac aagtggcgac gacggcaatc aggttaatcg gcagttgcag tttgacggcg
     4441 gcgcagaagg tgctgatgac ggttgccgcg ccgcacatat caaacttcat ttcgtccatg
     4501 ttcaggccgg gcttgaggga gatgccgccg gtgtcgaagg taatgccttt gccgaccaat
     4561 accacaggcg cggcttcttt gtcggctgca ccgaaatagc tcagttcgac caaatagggg
     4621 gcttccgcgc tgcctttggc gaccgaccaa aacgaaccca tgttttcttt gatgtagtct
     4681 ttttcgatga ttttggcgtg cgcgcccagt ttttcggctt cggctttggc ggtgcgcgct
     4741 aaaaattcgg gcgtgcattc gttgggcgcg gcgttgccca agtcgcggca gaggctttgt
     4801 ccgtaaactt gcgcttcggc gacgcgcaag gcttctttaa cgacggcttc gtgtgcgctg
     4861 tggaacacgg cggtttcaaa tttggcgggc ttggcttctt ttttgtagcg gtcgaaacgg
     4921 taggcggcgt tgccgaacgc aatcgcaaac gcttcggcta cggcggcggc ttgcgcttca
     4981 tcgaaggcat ggatgtccac attgaccgtt tcctgatttt gcgcccattt ggcggcttcg
     5041 gcggcggcct tgttcaatgc ggcgcggccg gtgcttttca gacagcatac ggcaacagcc
     5101 tgcaaaccgt tgcctgtcgg gatttttgtg tcggcaaaat tttgaccttc ttcaagcgaa
     5161 gacaaaaggg caagggcggt cggatggttt aattgcgcct cttcggtgca gacaaataac
     5221 tgtgcgcctg cctgctgttc ctgcaagatt tcggtttttg tgctaaattc cacgtttatt
     5281 ctcctgattg agacggttgt cggtagtttt cggacggcct ttcgctcaaa agaccgtctg
     5341 aagacggctg gcacgattgt accccatttg aagcaccgtc tgaaaccttg cgcggacaat
     5401 ccgcctgcgc cgaaccgctt accgccccct gaccgcgatt ctatgattta tcaaagaaac
     5461 ctcatcaaag aactctcttt taccgccgtc ggcattttcg tcgtcctctt ggcggtattg
     5521 gtctccacgc aggcaatcaa cctgctcggc cgtgccgccg acgggcgtgt cgccatcgat
     5581 gccgtgttgg cattggtcgg cttctgggtc atcggtatga cgccgctttt gctggtgttg
     5641 accgcattta tcagtacgtt gaccgtgttg acccgctact ggcgcgacag cgaaatgtcg
     5701 gtctggctat cctgcggatt ggcattgaaa caatggatac gcccggtgat gcagtttgcc
     5761 gtgccgtttg ccgttttggt tgccgtcatg cagctttggg tgataccgtg ggcagagcta
     5821 cgcagccgcg aatacgctga aatcctgaag cagaagcagg aattgtcttt ggtggaggca
     5881 ggcgggttca acagtttggg caagcgcaac ggcagggttt attttgtcga aaccttcgat
     5941 accgaatccg gcatcatgaa aaacctgttc ctgcgcgaac aggacaaaaa cggcggcgac
     6001 aacatcatct tcgccaaaga aggtaacttc tcgctgaacg acaacaaacg cacgctcgaa
     6061 ttgcgccacg gctaccgtta cagcggcacg cccggacgcg ccgactacaa tcaggtttcc
     6121 ttccaaaaac tcaacctgat tatcagcacc acgccaaaac tcatcgaccc cgtttcccac
     6181 cgccgtacca ttccgaccgc ccaactgatt ggcagcagca acccgcaaca tcaggcggaa
     6241 ttgatgtggc gcatctcgct gaccgtcagc gtcctcctac tctgcctgct tgccgtgccg
     6301 ctttcctatt tcaacccgcg cagcggacat acctacaata tcttgattgc catcggtttg
     6361 tttttaattt accaaaacgg gctgaccctg ctttttgaag ccgtggaaga cggcaaaatc
     6421 catttttggc tcggactgct gcctatgcac atcatcatgt tcgtcatcgc aatcgtactt
     6481 ctgcgcgtcc gcagcatgcc cagccagccc ttctggcagg cggttggcaa aagtctgaca
     6541 ttgaaaggcg gaaaatgaac ctgatttcac gttacatcat ccgtcaaatg gcggttatgg
     6601 cggtttacgc gctccttgcc ttcctcgctt tgtacagctt ttttgaaatc ctgtacgaaa
     6661 ccggcaacct cggcaaaggc agttacggca tatgggaaat gctgggctac accgccctca
     6721 aaatgcccgc ccgcgcctac gaactgatgc ccctcgccgt ccttatcggc ggactggtct
     6781 ctctcagcca gcttgccgcc ggcagcgaac tgaccgtcat caaagccagc ggcatgagca
     6841 ccaaaaagct gctgttgatt ctgtcgcagt tcggttttat ttttgctatt gccaccgtcg
     6901 cgctcggcga atgggttgcg cccacactga gccaaaaagc cgaaaacatc aaagccgccg
     6961 ccatcaacgg caaaatcagc accggcaata ccggcctttg gctgaaagaa aaaaacagca
     7021 ttatcaatgt gcgcgaaatg ttgcccgacc atacgctttt gggcatcaaa atttgggcgc
     7081 gcaacgataa aaacgaattg gcagaggcag tggaagccga ttccgccgtt ttgaacagcg
     7141 acggcagttg gcagttgaaa aacatccgcc gcagcacgct tggcgaagac aaagtcgagg
     7201 tctctattgc ggctgaagaa aactggccga tttccgtcaa acgcaacctg atggacgtat
     7261 tgctcgtcaa acccgaccaa atgtccgtcg gcgaactgac cacctacatc cgccacctcc
     7321 aaaacaacag ccaaaacacc cgaatctacg ccatcgcatg gtggcgcaaa ttggtttacc
     7381 ccgccgcagc ctgggtgatg gcgctcgtcg cctttgcctt taccccgcaa accacccgcc
     7441 acggcaatat gggcttaaaa ctcttcggcg gcatctgtct cggattgctg ttccaccttg
     7501 ccggacggct cttcgggttt accagccaac tctacggcat cccgcccttc ctcgccggcg
     7561 cactacctac catagccttc gccttgctcg ctgtttggct gatacgcaaa caggaaaaac
     7621 gttgaaccaa tgccgtctga cccccttcgg acggcattgg tttttattat catattcctg
     7681 tagacagata gccgttccct attatagtgg attaacaaaa accagtacgg cgttgcctcg
     7741 ccttgccgta ctattgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
     7801 actatacatt acctgtcata acagttccat ttttgttaaa actagtctat gatagcggta
     7861 caaatattgt ttacaatatt taacgcaaat catttgcaac ccgacaaaag aaaaacagaa
     7921 aaaggaacaa agagatgtta gaagcctatc gtaaagccgc cgccgagcgc gccgccctcg
     7981 gcattcccgc cctccctttg aacgcgcagc aaaccgccga tttggttgag ctgctgaaaa
     8041 gcccgcccgc aggcgaaggc gagttcttgg tcgaactgct tgcccaccgt gttccgcccg
     8101 gtgtggacga tgccgccaaa gtcaaagcct cattcctggc tgccgttgcc gaaggcagcg
     8161 cgtccagccc gctgatctcc cccgaatatg cgaccgaact cttaggtacg atgctcggcg
     8221 gttacaatat ccacgcctta atcgaactct tggacgacga caaactcgcg cccattgccg
     8281 ccaaaggctt gaaacatacg cttctgatgt tcgattcctt ccacgacgtt caggaaaaag
     8341 ccgaaaaagg caacaaatac gcgcaagaag ttttgcaatc ttgggcagat gccgaatggt
     8401 tcgcctcacg cgccaaagtt cccgaaaaaa tcaccgttac cgttttcaaa gttgacggcg
     8461 aaaccaatac agacgacctc tcccccgcgc ccgacgcgtg gagtcgtccc gatattccgc
     8521 tgcacgcgct ggcaatgctg aaaaacccgc gcgacggcat cacgcccgac aaaccgggcg
     8581 aagtcggtcc gattaaattg ttggaagaac tcaaagccaa aggccatccg gttgcttacg
     8641 tcggcgacgt ggtcggtact ggttcttcac gcaaatccgc gaccaactcc gtcatttggc
     8701 ataccggcga agacattccg ttcgtgccga acaaacgctt cggcggcgta tgtttgggcg
     8761 gcaaaatcgc gccgattttc ttcaataccc aagaagattc cggcgcgctg ccgattgaag
     8821 tcgatgtatc tgctctaaaa atgggcgatg tcgtcgatat cctgccttat gaaggcaaaa
     8881 tcgtgaaaaa cggcgagact gttgccgagt ttgaattgaa atcacaagta ttgctggacg
     8941 aagtgcaagc cggcggccgt atcaacctga ttatcggccg aggtctgacc gctaaagcgc
     9001 gcgaagccct gaaactgcct gcctctactg cattccgcct gccgcaagcg cctgccgaaa
     9061 gcaaagctgg tttcaccttg gcgcaaaaaa tggtcggccg cgcctgcggt cttcccgaag
     9121 gacaaggcgt gcgtccgggt acttactgcg aaccgcgtat gacgacggtc ggctcgcaag
     9181 acacgaccgg cccgatgacc cgcgacgagt tgaaagactt ggcttgtttg ggcttctccg
     9241 ccgatatggt gatgcagtct ttctgccaca ccgccgccta tccgaaacct gtcgatgtaa
     9301 aaacccataa agaactgccc gcctttattt ccacccgtgg cggcgtgtca ctgcgtccgg
     9361 gcgacggcgt catccactcg tggctcaacc gcctgctact gcccgatacc gtcggcaccg
     9421 gcggcgacag ccatacccgt ttccccatcg gtatttcctt ccccgccggc tccggcttgg
     9481 ttgcctttgc cgccgcaacg ggcgtaatgc cgctcgatat gcccgagtct gtattggtac
     9541 gcttcagcgg caagctgcaa ccgggcgtaa ccctgcgcga tttggtgaac gccatcccgc
     9601 tgtacgcaat caaacaaggt ttgctgaccg ttgccaaagc cggtaagaaa aacatcttct
     9661 ccggccgcat cctcgaaatc gaaggcctgc ctgatttgaa agtggaacaa gcctttgaat
     9721 tgaccgacgc atccgccgaa cgctccgccg ccggctgtac cgtgaagctc aacaaagagc
     9781 cgattatcga gtacatgaaa tccaacgtcg tgttgatgaa aaacatgatt gccaacggct
     9841 atcaagaccc gcgcactttg gaacgccgca tcaaagctat ggaaaaatgg ctggcaaatc
     9901 ccgagttgct cgaagcggat aaagatgccg aatacgccgc cgtgattgaa atcaacatgg
     9961 acgacatcaa agagccgatt atcgcctgcc cgaacgaccc ggacgacgtg tgcttcatgt
    10021 ccgaacgctc cggcaccaaa atcgacgaag tattcatcgg ttcgtgtatg accaacatcg
    10081 gccacttccg cgccgcctcc aaacttttgg aaggcaaggc agacaccccc gtccgcctgt
    10141 ggattgcgcc gccgaccaaa atggacgcga aacaattgtc cgacgaagga cactacggcg
    10201 tactcggacg tgccggcgcg cgtatggaaa tgccgggttg ctccttatgt atgggtaatc
    10261 aggcgcaagt acgcgaaggt gcgaccgtta tgtccacctc cacccgcaac ttcccgaacc
    10321 gtttgggtaa aaacaccttt gtttacctcg gttcggcgga attggcagcg atttgctcca
    10381 aactgggtaa aatcccgacc gttgaagaat atcaagccaa tatcggcatc atcaacgaac
    10441 agggcgataa aatctaccgc tatatgaact tcaacgaaat cgacagctac aacgaagtag
    10501 ccgagaccgt gaacgtttaa tccccgtcat ccgtatgaag taagggattg accgcaatgc
    10561 cgtctgaaca accttcagac ggcattgcaa cattccgcta acccttcttt ccgcaaacgc
    10621 tgcaaatacg gcgttcacgc ccccacataa aggaaacgac agtgaacctg aaaaaccgcc
    10681 attttctgaa acttttagac ttcacgccgg aagaaatcac cgcctacctc gaccttgccg
    10741 ccgaattgaa agccgccaaa aaagcagggc gcgagattca gcggatgaaa gggaaaaaca
    10801 tcgccctgat ttttgaaaaa acctctactc ggacgcgctg cgcgtttgaa gtcgccgcgc
    10861 gcgatcaagg cgcgggagtg acttatttag agccgtccgc cagccaaatc gggcataagg
    10921 aaagcatcaa agacaccgcc cgcgtgttgg gcaggatgta cgatgccatc gaatatcgcg
    10981 gtttcggtca ggaagttgtt gaagaattgg cgaaatacgc gggcgtaccc gtgttcaacg
    11041 ggctgaccaa cgagttccat cccacacaaa tgcttgccga cgcactgact atgcgcgaac
    11101 acagcggcaa acctttgaac caaaccgcgt ttgcctacgt cggcgacgcg cgttacaaca
    11161 tgggcaattc cctgctgatt ttaggggcaa aattggggat ggacgtgcgt atcggcgcac
    11221 cgcaaagcct gtggccgtct gaaggcatta ttgccgccgc acacgccgcc gccaaagaaa
    11281 ccggcgcaaa aattaccctg accgaaaacg cgcatgaagc cgtgaagaat gttgatttta
    11341 ttcataccga tgtgtgggtc agcatgggcg agccgaaaga agtctggcag gaacgcatcg
    11401 atttgctgaa agattaccgc gttacgcccg aactgatggc ggcatcgggc aatccgcaag
    11461 tcaaattcat gcactgcctg cccgccttcc acaaccgcga aaccaaagtc ggcgaatgga
    11521 tttacgaaac cttcgggctg aacggtgtgg aagttacaga agaaatattc gaaagccccg
    11581 ccagcatcgt gttcgatcag gcggaaaacc gtatgcacac gattaaagcg gtaatggtcg
    11641 cggctctggg cgactgacag aactgtgcct gtttaaattc atccgcaaca cagataccgt
    11701 ctgaacacga tgttcagacg gtatccatat atagtggatt aaatttaaac cagtacggcg
    11761 ttgcctcgcc ttgccgtact atttgtactg tctgcggctt cgtcgccttg tcctgatttt
    11821 tgttaatcca ctataaaaaa actgcctaca cgatgtgtag gtagtcccgt ttgaaaacaa
    11881 tcagtttttg tcttggtcaa ccaatttgtt ggcagtaatc caaggcatca tggcacgcag
    11941 ttgtgcgccg actttttcaa cttggtggtc ggcattcaga cggcggcggg cagtcataga
    12001 cgcatagttg acattaccct cttggataaa catttttgcg tattcgccgg tttgaatgcg
    12061 tttcagggca ttgcgcatgg cttctttgct ggaagcattg accacttcag ggccggtaac
    12121 gtattcgccg tactccgcat tgttggaaat ggagtagttc atattggcaa taccgccttc
    12181 gaaaatcagg tcaacaatca gtttcatttc gtgcagacat tcgaagtaag ccatttcagg
    12241 cgcgtaaccg gcttcggtca gggtttcaaa acccgccttg atcaactcga ccacgccgcc
    12301 gcacaatacg gcttgttcgc cgaacagatc ggtttcggtt tcttcgcgga aagtggtttc
    12361 aatcacaccg cctttggtgc cgccgttggc agccgcataa gacagggcga tgtctttggc
    12421 tttgccggaa ttgtcttggt aaacggcaat cagagaaggc acgccgccgc cgcgtttgta
    12481 ttcactgcgt acggtatggc ccggaccttt gggggcaacc ataatcacgt ccaagtcggc
    12541 acgcggaacg atttggttgt agtgcacgtt gaagccgtgt gcaaatgcca gcgttgcgcc
    12601 ttctttcaaa ttggctgcaa cttcggcgtg atagacggca ggcatggttt cgtcaggcag
    12661 cagcagcata acgacatcgg cttctttggt cgcttcagca acggttttga cgacatggcc
    12721 ggctgcttcg gcttttttcc aagaagaacc ttggcgcaga ccaatcacca cgtttacacc
    12781 cgaatctttc aggttggcgg catgggcatg accttgcgaa ccgtaaccga tgatggcaac
    12841 ggttttgcct ttgattaggg acagatcggc atctttatcg taatagactt gcatttgatt
    12901 tcctttaagg taaatggttg tcgaagcctt aaaatgttga gcggcttcgg acgggttaaa
    12961 cagagtgtgc cgcttaatcg gcaacttcat tcatcaatac gatttccaac gcttcggttt
    13021 tgccgtcgac ggactggacg aaggcttgga aatgcgcgct ggcgttatgt tcgtcaatag
    13081 ctgcttgaga tttccaattt tccacgaaaa caaaacggtt cggtttgccg atttcctgat
    13141 ggagatcgta gctgatgttg ccctcttctg cacggctggc tttgaccagt tctttaaact
    13201 gtgctgccag tgtttctgtg tattccggtt tgacggtaac cagtgcgaca attttaatgt
    13261 tcgacataaa tctctcctgc cgttcgtttt tcagacggca ttcaaatacc gtgccgtctg
    13321 aaaggttacg gcgttaaatt ttcaaaatac gctcaccgcg accgatgccg gccgcgcctg
    13381 tgcgtacggt ttccaaaatt tgggcgcgtc cgaccgtttc caaaaaggaa tccagcttgt
    13441 ctgtcgagcc ggtaatttca atcgtatagc tgcggtcggt tacgtcgatg atgctgcccc
    13501 ggtagatttc ggtcaagcgt aaaaattcgt cgcggtcttt gccggcggca cggactttta
    13561 ccaacatcag ttcgcgttcg acaaaacggc tttcgttcaa atcgaccact ttaatcactt
    13621 caatcaattt attgagttgc ttggtgattt gttcgatgac ctgctcgtcg ccgtgggtaa
    13681 cgatggtcat acgggacagg gttttgtctt cggtcggcgc aaccgccaaa gaatcgatat
    13741 tgtaatcgcg tgcagagaac aaaccgacca cgcggctcat cgcacctgat tcgttttcaa
    13801 tcagaacaga taagatatgt cgcatttgcc tctccttacg ccttttcgtc cgcacgcata
    13861 tgcggcggaa gtaccatttc gtccaaacct ttgccgttac cgaccatggg catcacattc
    13921 tgtttctggt cggtcaggaa gtcgataaac accagcctgt ctttttggtt caatgcttcc
    13981 aacaacgcac cttccacatc agacttcttg tccacgcgga tgccgatatg accgtatgcc
    14041 tcggcaagtt tgacgaaatc gggcaaagaa tcgaaataag tttccgactc tcggccgccg
    14101 taatatattt cctgccactg gcgtaccata ccgagataac cgttgttcag cgtaatgacg
    14161 ttaaccggaa tccgatattg gaaacaggtg gacaactctt ggatgttcat ctgaatcgag
    14221 ccgtcgccgg taatacagaa tacgtcttga tccggggcgg caagttttgc accaatcgca
    14281 taaggcagac ccacgcccat cgtacccaaa ccgccggaat tgagccattg gcgcggacgt
    14341 tcgaagggat aatattgagc cgcaaacatt tgatgctgcc ctacatccga tgtgatgatt
    14401 gccgaattgc cggtaatctc ggcaagcttc tgaatcacat attggggctt gataatttcg
    14461 ctgccgttgt caaaccacaa gcaatctcgg gaacgccatt cctctatggt tttccaccat
    14521 ttgcccaaag tatcttcaga cggcacggac tcttgttttt gccacagcgc aaccatctcg
    14581 gacaaaacgt ttttcacgtc gccgacaatc ggaatgtccg ccttcacgcg tttggcgatg
    14641 ctggaaggat cgacatcgat atggataacc ttcttcgcct tctcgaaaaa tttggacggt
    14701 acggaaacca cacggtcgtc aaaacgcgca cctacggcaa gaacgacatc cgcattctgc
    14761 atggcaaggt ttgcctcgta agtaccgtgc ataccgagca taccgaggaa ttggcggtcg
    14821 ctggaaggat aagcgcccaa gcccatcagc gtacccgtgc acggagcacc cgtcattcgg
    14881 acaaatcggg tcagctcttc agaagcatta cccaacacca cgccgccgcc aaaatagacg
    14941 actggacgtt tggcagatgc caacatctgc acggcctttt taatctgacc gatatgccct
    15001 tgaacaaccg gttgatacga acggataaaa atgtcttcct gaggatagct gaatttcgcc
    15061 atcgcctgcg taacatcttt cgggacatca accaccacag gccccggtcg gccgcttgcg
    15121 gcaatttgga acgccttttt aatggtttcc gccaactcat tgatatccgt aaccaagaaa
    15181 ttgtgtttga cgcacggacg ggtaataccc accgtatcaa cttcttggaa cgcatccgta
    15241 ccaatcaggg aattgcctac ctgcccgctg atgaccacca tcggaatcga atccgtatag
    15301 gcagtagcaa taccggtcag tgcattggta acgcccgggc cggatgtaac caatgccacg
    15361 cccaccttac cgctgacgcg cgcatacgca tctgccgcgt gtactgccgc ctgctcgtga
    15421 cgggtcagaa tgtgcttgaa tttattgagt tggaaaaggg catcgtagat ttcgataacc
    15481 gcaccgccgg gataaccgaa aacgtactcg acaccttcgg ctttgagact ctgcactatg
    15541 atttgcgcgc ctgatagctg cataacgacc tcttttatac ggtttcaaac caatagggac
    15601 aaaccgcttt gccacagcac ctgtaatgca attccaccaa gcagcgattt agggtacgcg
    15661 cattggggga acacggcaac agacggatta tccaatcaat tggaaaggaa cacagagttt
    15721 gtgaaaaaga gtggaaacga taacgcaaac cgacagttca atcaagaaaa atctttcatc
    15781 ttttaatatt ttttgaaagc agagaaatta ttgattgatt ttaaaagaat aaaatcagga
    15841 gtaccttttt tgaaagatgg aaattgttga cagtttgtgt aggaggggca gatgtgaaaa
    15901 acccttcttc gatatcaaga attgtaaaat ttacagggtt tcatcccaat aaagactcgg
    15961 gatattgatt gaacttgatt tcatttttga tatatcaaaa atattcccaa ccatacttcc
    16021 tgaaaatggc tcattgcacc ggactgtatt ggacggcatt gacagaacca agagggctaa
    16081 caacgactta atatattgat tgtatagtgg attaacaaaa atcaggacaa ggcgacgaag
    16141 ctgcagacag tacaaatagt acggcaagga gaggcaacgc cgtactggtt taaatttaat
    16201 ccactatatt tagtcttatc tatttcatta aacagcaata gacaaaaaaa taaccgctcc
    16261 aaaagcggtt gtggtgccca gggtcggact cgaaccgaca caccttgcgg cgggggattt
    16321 tgagtcccct gcgtctacca atttcgccac ctgggctggt gaagaagtcg tcattataat
    16381 ggcttttgaa attctgtaaa cctttttttg aaattatttt atctgttttt attttatttt
    16441 tgattttaaa tagaattttt attattttaa tcttactgtt ctttccgctc caaagattct
    16501 gtatgattcg gcaattcctg ccgtgcagac aacgtaaaaa aatactacat taaatctgcc
    16561 aaacgcgtta agatgaaaat attcaaattc cgtacgaatc aggttttgct atttattctt
    16621 gggagattgt catgttttcc gtaccgcgtt cctttttgcc gggcgttttc gtacttgccg
    16681 cgcttgccgc ctgcaaacct caagacaaca gtgcggcgca agccgcttct tcaagtgcat
    16741 ccgcgtcagc tgcggaaaat gcggcaaagc cgcaaacgcg cggtacggat atgcgtaagg
    16801 aagacatcgg cggcgatttc acgctgaccg acggcgaagg caagcctttc aacctgagcg
    16861 atttgaaagg caaggtcgtg attctgtctt tcggctttac gcactgtccc gatgtctgcc
    16921 cgacagagct tttgacgtac agcgacacgt tgaagcagtt gggcgggcag gctaaggacg
    16981 tgaaagtggt gttcgtcagc atcgatccgg aacgcgacac gcctgaaatc atcggcaagt
    17041 atgccaaaca gttcaatccg gactttatcg gtctgacggc aacgggcggc caaaacctgc
    17101 cggtcatcaa gcagcaatac cgcgtggttt ctgccaaagt caatcaaaaa gacgacagcg
    17161 aaaactattt ggtcgaccac tcttccggtg cgtatctcat cgacaaaaac ggtgaggttg
    17221 ccattttctc gccttacgga agcgagccgg aaacgattgc tgccgatgta aggaccctgc
    17281 tctgataaaa ccgtatgccg tctgcaccgt cggcgcctat tcagacggca ttattgtttc
    17341 aaccgacaaa ggacatccac accatgcagg ataatgcttt gaccatcgcc ttatccaagg
    17401 ggcgcatttt tgaggagacg ctgccgctgc ttgccgctgc cggcattgtt ccgactgaag
    17461 agcctgaaaa atcgcgcaag ctgattatcg ggacgaacca tgaaaacatc cgccttgtca
    17521 ttgtccgcgc aaccgatgtg ccgacttatg tccgctacgg cgcggcggac ttcggcattg
    17581 cgggcaaaga cgtgctgatc gaacacggcg gcacggggct ttaccggcct ttggatttgg
    17641 agattgccaa gtgccgcatg atggttgctg tgcgtaaagg gtttgattac gaagcagctt
    17701 cgcaacccgg atgccgtctg aagattgcca cgaagtatcc tgaaatcgcg gcatctcatt
    17761 ttgccggcaa gggtgtccat gtggacatta tcaaactgta cggctcgatg gaacttgcgc
    17821 cgctggtcgg cttgagcgat gcgattgtgg acttggtttc gacgggcaac accttgaagg
    17881 caaacggctt ggaagcagtc gaacacatcg tcgacatttc cagccgcctg gtggtcaaca
    17941 aggctgcttt gaaaacgaaa tacgcgctgc tggagccgat tattcaggcg ttcggcggcg
    18001 cagtgaaggc gaagtaagca tccatttgaa taaagatgcg ttttcagacg accctatccg
    18061 ttcccgccga caggtcgtct gaaaatatca ccggcagtaa actgtatagg agaagttaaa
    18121 atggttgcaa aaataaaaaa attctcagat tcaacccttt ccgttttgaa taacggcgag
    18181 cgtcggtttt atgtctattg tctgaccgac ctgaaaaaag acaaaatcct ctacatcggc
    18241 aaaggctgcg gtaatcgtat cttcgagcat gaatgggttg ctagtcgttc acaagaccca
    18301 gtctccggcg agattatcga tcggaaactc aaagccatct ccaaatgcaa gaaactcggt
    18361 cgctatatca tcagctatca tctgactgaa gtcgaagcac tcgccgccga atctgcctta
    18421 attcattttg ttaaatctgt cttgggtaaa aaactcaaaa ataaaattgc cgggcatggt
    18481 ccgggtggta ttagcgtaga agaactagat cgccgctttg gattctcttc tctcccactt
    18541 aacgagatta accccgacgg gctgattctc gccatcaaaa tccacaatgc tttcgattta
    18601 gatactgacg aagaattaga ctaccttttc gacaaccaag acgatgccaa cctcaaatcg
    18661 cgtacgttgg gcaactgggt tatcggtaaa gatgttgctt caaaagtgaa atacgttatc
    18721 ggcgttcaca ccggtctgca aaacgctgtt gtcagtgcat acgaagtgga cggttttgaa
    18781 acaatggttg aggaaaccaa aaacggtaga aaacaatccc gttaccgttt ccgcactacc
    18841 tctcgtagcg aagaggtatt agccaaactc ggtctgcaac aaaaatgcct gcccgaattg
    18901 aagtttggta gcgggggaga aaaagcgtat atcagaccca aaacagagac agaaactgaa
    18961 caagagaata ttcagacgac ccccaatcca aaaataaaaa aggaaaaaac caaatcatga
    19021 aaaaactcaa cacccaatcg cccgatttcc aagccggact caaagccctg ctggcttttg
    19081 aaaccgcgca aaaccccgaa accgaacgca tcgtcgccga catttgcgcc gacgtgcaaa
    19141 agcgcggcga tgcggctttg attgaataca ccaacaaatt cgatcagaca aacgctaaaa
    19201 gcatcgatga tttaatactc acgcaagccg atttgaacgc ggcgttcgag cgcattccga
    19261 acgacgttca gacggcattg cagaccgccg cccgccgtgt cgaaagctac caccaacgcc
    19321 aaaaaatgga atcgtggagc tacaccgatg aagacggcac gctgttggga caacagatta
    19381 ccccgcttga ccgcgtcggc atttacgtcc ccggcggcaa ggcggcatat ccgagttccg
    19441 tcatcatgaa cgccatgccc gcccacgtcg caggcgtgaa agaaatcatc atggtcgtgc
    19501 cgacaccgaa aggcgaacgc aacgacatcg tgcttgccgc cgcatacgtc gccggcgtaa
    19561 ccaaagtctt caccgtcggc ggcgcgcagg cggttgccgc cctcgcctac ggcacggaaa
    19621 ccatccccca agtcgataaa atcaccggtc cgggcaacgc cttcgtcgcc gccgccaaac
    19681 gccgcgtgtt cggcgtggtc ggcatcgaca tggtggcggg gccgtctgaa atcctggtca
    19741 tcgccgacgg cacgacacct gccgattggg tggcgatgga tttgttcagc caggccgaac
    19801 acgacgaaat tgcccaagcc atcttaatcg gcacgtcgca agcgtatctc gacgaagtag
    19861 aagccgctat ggaccgcctg atcgaaacta tgccgcgccg cgacatcatc gaagcctcgc
    19921 tcggcaacag gggcgcgatg atactcgcca aagacttgga cgaagcctgc gaaatcgcca
    19981 actacatttc ccccgaacac ttggaactgt cagtcgaaaa tccgcaggaa tgggcgaaaa
    20041 aaatccgcca cgccggtgcg attttcatgg gacgctacac cggcgaaagc ctcggcgact
    20101 actgcgccgg tccaaaccat gtattgccca ccagccgaac cgcccgcttt tcctcgcctt
    20161 tggggacata tgatttccaa aaacgctcca gcctgattca ggtttcggaa cagggcgcgc
    20221 aaaaattagg cgaaaccgcc agcgtgctgg cacacggcga aagcctgacc gcccacgccc
    20281 gcgcggcaga gttccgtatg aaataatgcc gaaacggcgt acaggcatat tccaaccatt
    20341 aaggaaacac gatgaaatcc gtccgctcct tcatccgcga cgacatacaa gctatgtcgg
    20401 catatcagat tgccgacgtt ccgcccggct ttgccaaact cgattcgatg gaaagtcccg
    20461 tccacccttt tgccggacat gaaacgctgt tgcaggaatg gcaggcacgg cttgccgccg
    20521 cgcccatcca tctttacccc aatccctccg gcagcggttt acaggaagca ttacgttcgg
    20581 cgttcgacat tcccgactgc gccgacatcg cgctgggcaa cggctcggac gagctgatac
    20641 agttcatcac gatgctgacc gccaaaccgg gcgcggcaat gttggcagcc gaacccagtt
    20701 tcgtcatgta ccgccacaac gccgcgctgt acggcatgga ttatgtcggc gttccactga
    20761 acggagattt caccctcaac ctgcccgccg tcctcgaagc cgtcaggaag caccgccctg
    20821 ccctgacctt tatcgcctac cccaacaacc ccaccggcgt atgtttcacg cgtgccgaaa
    20881 tcgaagccgt catcgaagct tcagacggca tcgtcgtcgt cgacgaagcc tacggcgcat
    20941 tcaacggcga cagcttcctg ccgcaggcgg gcagcattcc caacctcatc gtcatgcgta
    21001 ccgtcagcaa aatcggtttt gccggactgc gtatcggcta tgcggcaggc tgtcccgaag
    21061 tcatcggcga actgcaaaaa atcctgccgc cctacaatat gaaccaactg agcctgacca
    21121 ccgccaaact cgccctgcaa cactacggca tcatctctgc caacatcgac agcctgaaaa
    21181 acgaacgcga acggatgttc gccgaattgg gcaaaatatg ccgtctgaac acctttccaa
    21241 gtcaggcaaa cttcattacc atacgcgtac ccgatgccga tttgttgttt gacacgctca
    21301 aacaaaaccg catcttggtt aaaaaactgc atggcgcgca cccgcttttg gaacactgcc
    21361 tgcgcattac cgtaggcagc cccgcacaaa acgatgccgt tctcaacatc attcgccaac
    21421 tttactgcca accaacggat ttcctatgaa tttgactaaa acacaacgcc aactgcacaa
    21481 ctttctgacc ctcgcccaag aagcaggttc gctgtccaag ctcgccaaac tctgcggcta
    21541 ccgtaccccc gtcgcactct acaaactcaa acaacgcctt gaaaagcagg cagaagaccc
    21601 aaatgcacgc ggcatccgtc ccagcctgat ggcaaaactc gaaaaacaca ccggcaaacc
    21661 caaaggctgg ctcgacagaa aacaccgcga acgcactgtc cccgaaaccg ccgcagaaag
    21721 caccggaact gccgaaaccc aaattgccga aaccgcatct gctgccggct gccgcagcgt
    21781 taccgtcaac cgcaatacct gcgaaaccca aatcaccgtc tccatcaacc tcgacggcag
    21841 cggcaaaagc aggctggata ccggcgtacc cttcctcgaa cacatgatcg atcaaatcgc
    21901 ccgccacggc atgattgaca tcgacatcag ctgcaaaggc gacctgcaca tcgacgacca
    21961 ccacaccgcc gaagacatcg gcatcacact cggacaagca atccggcagg cactcggcga
    22021 caaaaaaggc atccgccgtt acggacattc ctacgtcccg ctcgacgaag ccctcagccg
    22081 cgtcgtcatc gacctttccg gccgccccgg actcgtgtac aacatcgaat ttacccgcgc
    22141 actaatcgga cgtttcgatg tcgatttgtt tgaagaattt ttccacggca tcgtcaacca
    22201 cagtatgatg accctgcaca tcgacaacct cagcggcaaa aacgcccacc atcaggcgga
    22261 aaccgtattc aaagccttcg ggcgcgccct gcgtatggca gtcgaacacg acccgcgcat
    22321 ggcaggacag accccctcga ccaaaggcac gctgaccgca taaaaaacca taccgtctga
    22381 aacacccgca ggcttttcag acggtatcgg aacagataag attacactac actacaaaca
    22441 gaaaaggagt aaacatcatg tccgcaaacg aatacacaca aatcggctgg ataggcttag
    22501 ggcaaatggg tctgcctatg gtaacgcggc tcttggacgg cggcatcgaa gtcggcgtat
    22561 acaaccgctc gcccgacaaa actgccccca tctccgccaa aggcgcaaaa gtttacggca
    22621 acaccgccga actcgtccgc gactatcccg tcattttcct gatggtttcc gactatgccg
    22681 ccgtgtgcga catcctgaac ggagtccgcg acggattggc cggcaaaatc atcgtcaaca
    22741 tgagcaccat ctccccgacc gaaaacctcg ccgtcaaagc acttgtcgaa gccgcaggcg
    22801 gacagtttgc cgaagcaccc gtttccggat cggtcgggcc cgccaccaac ggcacgctgc
    22861 tgattctgtt cggcggcagc gaagccgttt taaacccgct gcaaaaaata ttttccctcg
    22921 tcggcaaaaa aaccttccat ttcggcgatg tcggcaaagg ttcgggcgcg aaactcgtct
    22981 tgaactcgct cttgggcatt ttcggcgaag cgtacagcga agcgatgctg atggcgcggc
    23041 agttcggcat cgataccgac accatcgtcg aagccatcgg cggctcggca atggactcgc
    23101 ccatgttcca aaccaaaaaa tccctgtggg caaaccgcga attcccaccc gccttcgccc
    23161 tcaaacacgc ctccaaagac ctcaacctcg ccgtcaaaga gcttgaacag gtaggcaaca
    23221 ccctgcccgc cgtcgaaacc gttgctgcca gctaccgcaa agcagtcgaa gccggctacg
    23281 gcgaacagga cgtttccggc gtttacctga aattggcaga acactgattg ccttttccaa
    23341 acacaatgcc gtctgaacat atttcagacg gcatttttat caccccacgc ttaaaatcag
    23401 tcccgattat ga
//
LOCUS       DAWHXZ010000005        22076 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000005 DAWHXZ010000000
VERSION     DAWHXZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22076
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.005"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(145..891)
                     /locus_tag="WIE35_000132"
     CDS             complement(145..891)
                     /locus_tag="WIE35_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ4153121.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(1070..1741)
                     /locus_tag="WIE35_000133"
     CDS             complement(1070..1741)
                     /locus_tag="WIE35_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ4153122.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1810..2481)
                     /gene="rpiA"
                     /locus_tag="WIE35_000134"
     CDS             complement(1810..2481)
                     /gene="rpiA"
                     /locus_tag="WIE35_000134"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ4153123.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFICIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLMMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2558..3040)
                     /locus_tag="WIE35_000135"
     CDS             complement(2558..3040)
                     /locus_tag="WIE35_000135"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ4153124.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDEDSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(3072..3761)
                     /locus_tag="WIE35_000136"
     CDS             complement(3072..3761)
                     /locus_tag="WIE35_000136"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ4153125.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPVADTLKRADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDVRNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3758..4492)
                     /gene="dnaQ"
                     /locus_tag="WIE35_000137"
     CDS             complement(3758..4492)
                     /gene="dnaQ"
                     /locus_tag="WIE35_000137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ4153126.1"
                     /translation="MIQRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGKVYLAMTRRQFDLMGEAEEEEPAAKPVASAEMKLGAKLKVIKADEAELAAHG
                     QYLDGLGEACIWRKEAVPSENGGADA"
     gene            complement(4650..5960)
                     /locus_tag="WIE35_000138"
     CDS             complement(4650..5960)
                     /locus_tag="WIE35_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ4153127.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRYKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5950..6135)
                     /gene="ccoS"
                     /locus_tag="WIE35_000139"
     CDS             complement(5950..6135)
                     /gene="ccoS"
                     /locus_tag="WIE35_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ4153128.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6135..6434)
                     /locus_tag="WIE35_000140"
     CDS             complement(6135..6434)
                     /locus_tag="WIE35_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153129.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6785..7981
                     /locus_tag="WIE35_000141"
     CDS             6785..7981
                     /locus_tag="WIE35_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ4153130.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTAGSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(8617..10458)
                     /locus_tag="WIE35_000142"
     CDS             complement(8617..10458)
                     /locus_tag="WIE35_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ4153131.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPRAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            10549..11019
                     /locus_tag="WIE35_000143"
     CDS             10549..11019
                     /locus_tag="WIE35_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ4153132.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSVLEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(11090..11902)
                     /gene="hpnC"
                     /locus_tag="WIE35_000144"
     CDS             complement(11090..11902)
                     /gene="hpnC"
                     /locus_tag="WIE35_000144"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ4153133.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLDALRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(11989..12471)
                     /locus_tag="WIE35_000145"
     CDS             complement(11989..12471)
                     /locus_tag="WIE35_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ4153134.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDSELVRIEDVGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            12944..13210
                     /locus_tag="WIE35_000146"
     CDS             12944..13210
                     /locus_tag="WIE35_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153135.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPAAEAAAT
                     EAPAAEAPTAEAAATEAPAAEAPAAEAAATEAPAAEAPAAEAAK"
     gene            13320..14687
                     /locus_tag="WIE35_000147"
     CDS             13320..14687
                     /locus_tag="WIE35_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ4153136.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLIIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(14868..16853)
                     /locus_tag="WIE35_000148"
     CDS             complement(14868..16853)
                     /locus_tag="WIE35_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ4153137.1"
                     /translation="MISLFFSILYTSPLLAVDYVYDNSKLTNEQIDRLKKLRDKSSEY
                     WKQETYLLKPTNGSSQNSLKVPALYSKTHQFKNINNSKKISFYDKEYTEDYLVGFARG
                     FGVEKRNGEEEKTLQQYFKDCVNTGNSNNDKCEQPSFENSGPRLIKSDIFALAPQIKN
                     SHINSEILSVGNYIEWLRPTLNKLPSWQEHLYSGLDPFHYIEVTDNSHVIGQTISLDE
                     FRLENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKEDYAGGARFRFAYDLKDKVS
                     ETPVLTFEKNITGTSDIIFENPIDDLKSLDGHQIIKVNGTADKHAFRLSGKHQKGIYT
                     LSLQQRPEGFFTKVQERDDISIYAQQAQAANTLFALRLNDKNSDIFDRTLPRKGLWLR
                     VIDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQLSVGLMSGQAEQRSTFRNP
                     DTDNLTTGNVKGFGAGIYATWHQLQDKQTGAYADSWVQYQRFRHRINTEDATERFTSK
                     GITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQLTYLGVNGKFSDSENAHVNLLGSR
                     QLQTRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVEIDGERRVINNKTAIESQL
                     GVAVKIKSHLTLQASFNRQTSKYHQAKQGALNLQWTF"
     gene            complement(17269..17715)
                     /gene="smpB"
                     /locus_tag="WIE35_000149"
     CDS             complement(17269..17715)
                     /gene="smpB"
                     /locus_tag="WIE35_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ4153138.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(17770..18780)
                     /gene="waaF"
                     /locus_tag="WIE35_000150"
     CDS             complement(17770..18780)
                     /gene="waaF"
                     /locus_tag="WIE35_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ4153139.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAVIALAT
                     GIAKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(18911..18986)
                     /locus_tag="WIE35_000151"
     tRNA            complement(18911..18986)
                     /locus_tag="WIE35_000151"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18951..18953),aa:Asn,seq:gtt)
     gene            complement(19177..19929)
                     /locus_tag="WIE35_000152"
     CDS             complement(19177..19929)
                     /locus_tag="WIE35_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ4153140.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEVRYPDSLNELVCAAIATPLGEMLAVFGGKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFQCRLKTFATPLEMI
                     GTAFQKQAWDALLAIPYGETRSYKEQAQHLGNPKAVRAVAAANGQNKVSVLIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            20240..20764
                     /locus_tag="WIE35_000153"
     CDS             20240..20764
                     /locus_tag="WIE35_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ4153141.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGDNAPPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVRRRN
                     AASLQLSRCASIADDPSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSAASA
                     ALCGTSSSVSTAAA"
     gene            complement(20853..21132)
                     /locus_tag="WIE35_000154"
                     /pseudo
     CDS             complement(20853..21132)
                     /locus_tag="WIE35_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(21504..>22076)
                     /locus_tag="WIE35_000155"
     CDS             complement(21504..>22076)
                     /locus_tag="WIE35_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEZ4153142.1"
                     /translation="KQGHIAYPHLAINPVHTFAPALLELTQEVWDEGNEYFPPTSFQI
                     SNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAILDKHGVQYDLQWSCSGQ
                     PFLTQAGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKAIAKELIELGPSNATIHQ
                     INENVRLDDIPKLSAVYEGILARLLAGNAV"
BASE COUNT         5467 a         6314 c         5096 g         5199 t
ORIGIN      
        1 ggggcttcag caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg
       61 gtttttgtta atccacgata aatttgccac aaaaaagctg cctcaaatga atacccgggc
      121 agctttttgt tgatatgact ccaatcagcg gtgttgcgga ttgtaacgtt tttccaaacg
      181 caggaatatc cagcctaaga aagtcgtcat caacagataa atcagggcga cggtgtaaag
      241 cggttcttca taaaccgaat accggcccgt aatcgtattc tgaacatacg ccaactccgc
      301 cacagcaatg accgacagca gcgagctgtc tttcaagagc gtgatgaact cgctcgccaa
      361 aggcggcagc atgcggcgca atgcctgcgg cagaatcaca tagcgcatcg cctgcggata
      421 ggtcagcccc aaagaacgcg ccgcctccat ctgtcctttg tctatagact ggatgcccgc
      481 gcggaaaatc tcacagatat acgcccccga gttggcgatc agtgccaaag aaccggcaat
      541 cagcggcccg tatccgcgac gcagcgcgat tgccgcctcg ccgctgacca aaatgccgtc
      601 tgaaggatgg acgaaaaacg gaaaccacac atacgcccaa atcacaatct gcacaaacag
      661 cggcgtaccc cggaacagcg taacatacag cagcgaaact ttacgcaacg cccacgccag
      721 cacgcgcatc ggcgcaccgg ctttttccaa gtgaatcagg cgcgccaacg ccaacaacag
      781 acccaatacc gaaccgcccg ccgtcgccac gaccgtcagc cccaaggtcg tcagtgcgcc
      841 gtaaaggaac atccagcggt attcgtaaat aatgtcaaaa cgaaaatcca tataccgtcc
      901 gtatcaaaaa ccggcggaac tgccgccgtt gcaaaataat ccgccatttt accgtaaaaa
      961 ccgccgcctg aactttttta tcgcggcaga cggcggttgc gcgtctccgc aaaaatgcag
     1021 ggcgcgcggt tttcagacgg catttgccgt tcaaagccgt gcggtgtctt taccaaatgc
     1081 ccaaccattc gcccacggca tccatccaat ccttattgcc cccgccgctc ctgcctgctc
     1141 ggcggtacgc ccacggcgct tgcggatttt tagctttcca caatcctttg cgttcccttt
     1201 ccgcctgaat ttgagcgtcg gcatagtcgg caaaatccgc cttatcctgc tgttctttag
     1261 cataactttt ataatgccac gccgccccgt cctgcacctg catcaggttc aaatcggttt
     1321 tgccgacaga aacctgcgcc acttcgcgct ggtagcggtc ggtgtcgaac acgcgcacgc
     1381 tgactttcct gccttccgcc gccgcgcgca ggttgtcgcg cgaacgcgtg ccgtaagcct
     1441 gtttcatctc cggcgcgtcg atatacgcca tccggatttt gtgtttcgcg ccgtcgccgt
     1501 cgataacgtg aagggtgtcg ccgtcataga ctttggacac cgtgcctgtg tagcggtggc
     1561 cggatttcgc cgatgctcgg cggcgggcgg gcgcgtcgga acccgcgtcc cctgccgcgc
     1621 cgagtacgtc gagtacggca accgccgtcc gcaccgcctc gctgccgtac cccgtataac
     1681 ccaacgcacc caaaagcgac agggcgacgg gaagccattt catgattttt ttaatctgca
     1741 tatttttcaa atgccgatgc cgtctgaaca tctctgaacc ggatttcaga cggcatctta
     1801 acgtcaggat tacccttggc agggatagat gactttcgca ccctcttccg tccccaaaat
     1861 caacacatcg gcggcatcgc gggcgaatat gccgttttcg agtacgccgg tgattttgtt
     1921 gatttcgtct tccatcatca gcggtttgtc gatattcaag ccgtggacat cgacgatttg
     1981 gttgccgtaa aacgtggtgt agccgatacg cagttcgggc tgtccgccca tagcgagcag
     2041 tttgcgcgaa acaagggaac gcgcgctttc gacgacttcc actggcagag ggaatttgcc
     2101 caaacgtgaa acatatttgc tttcatccgc aatgcagatg aatttttctg atgcgctggc
     2161 gacgattttt tcgttgaggt gcgcgccgcc gccgccttta atcatttgca gggcgtggtt
     2221 cacttcatcc gcaccgtcga tatagaccgc caaccccgat acttcgttca aagaaacgac
     2281 gggaatatca tattgggcaa gcagttcgct ggattttttg gaagtagata ccgcgccttt
     2341 gatttttttg ccgctcttac ccaaggcttc gatgaaaaag ttgatggtcg agccggtacc
     2401 gatgccgatg tattcgtttt cgggtacgaa ttcgactgct ttttcggcgg cgatgcgctt
     2461 gagttcgtct tgtgtcgtca tatttttgtc ctttgggaaa ccgtatcaac aaacagccgt
     2521 catcttaaca tttttttgca cgtcctgccc gccgcgttca aatccgtacc agcaataccg
     2581 ccgcctgcgc ctctatgcct tccatgcgcc cgagataacc gagtttttcg ttggttttgc
     2641 ctttgatgtt gacgcacgaa atgtctatgc ccaaatcggc ggcgatgttg gcacgcattt
     2701 gcggaatatg cggcgcgagt ttgggtttct gtgcaatcac ggtcgtatcg acattgaccg
     2761 cctgccaacc ctgcgcctga acgctttgat acgccgcacg caaaaggacg cggctgtcct
     2821 catctttgaa ctctgcggcg gtgtcgggga aatggctgcc gatatcgccc aaacctgccg
     2881 caccgagcag cgcgtcggta acggcgtgca gcagcgcatc ggcatcggag tgtccgagca
     2941 gccctttttc aaatgggatt tcaactccgc caagtatcag ctttctgcct tcggtcagtt
     3001 ggtggacatc gtagccctgt ccgatacgga tgttcgtcat cgtttgtgtt cctgatggtt
     3061 tgaattgaag ttcagacggc atcgagcagc agcctgacga tgtatgcgtc ctgcggctgc
     3121 gtcagtttca aattgcgcac gtcgccctgt atcagtagcg gacgcacacc caatttttcc
     3181 acggcggacg cttcatcggt aatgccgtcc aagttttccg cagccaatgc gcggtgcagc
     3241 agcccggcgc ggaaaagctg cggcgtttgc gcctgccaaa ggctcgtccg ctcgacggtt
     3301 gcactaatgt tcccaccgtc cgcgcgcttg agcgtatcgg caacgggaat tgccaaaatc
     3361 ccgccttcgg cggcgttgcc cgcctgttct atcaaacgcg tcagagcttc agacggcagg
     3421 cagcagcgcg cggcatcgtg taccaaaata ttgtcggttt ccgccgccaa accgatttcc
     3481 aacagttttg ccacaccgtt tcggacggtt tcggcgcggg tctgtccgcc gtttttccac
     3541 acccgaacct gtggaaatgc cgtctgaacc ttatcggcaa acgtgtcttc gggcgagacg
     3601 acaacgacgg tcaaatcgac gccctcatgc cgttcaaaaa tcccaatcgt atgttctaaa
     3661 acggttttgc ttccgatttc gacatactgc ttaggtttgt ccgcaccgaa acgcgccccg
     3721 atgccggcgg cgggaatcag cgcgatattt ttgcgcttca tgcgtccgcc ccgccgtttt
     3781 ccgacggcac ggcttccttg cgccagatac aggcttcgcc caagccgtcc aaatattgcc
     3841 cgtgcgccgc cagttcggct tcatccgcct taatcacttt cagtttggca ccgagtttca
     3901 tttcggcaga tgcaacaggt ttggctgccg gctcttcctc ctcggcctcc cccatcaggt
     3961 cgaactgccg ccgcgtcata gcaagataga ctttgcccaa aagttcgcag tcgatcaatg
     4021 cgccgtgcag gacgcgcttg ctgcggtcga cggaaaaacg gttgcacaag gcatccaggc
     4081 tggctttctg cccggggaac atttcgcgcg ccatcgccag ggtatcggta acggtacagc
     4141 cgagttcctc gacagtcggc agccccatcc ggcggaactc catattgagg aagcccacgt
     4201 cgaatttggc attgtggata atcagttccg caccgcgcag gaaatcggca atctgcctgc
     4261 cgacctctgc aaacggcggc gcgtttttcc cttccaaaac ctctatcgtc agaccgtgca
     4321 ccctcgccgc ctcttcgggc atatcgcgct cggggtggac atagaggtgc aggtttttgt
     4381 cggtcatttg gcggttgacc atttctaaac cggcaaactc gaccaagcgg tcgccgccgt
     4441 cggcatacag accggtggtt tcggtatcga ggatgatttg gcgttgaatc atgtctgctt
     4501 ctttcttcaa attttccatc tgctgactat tttttaagca atatattttt ctgttttcat
     4561 ttcaatgcac aaacccactt attcacagtg tgttcacaac attgggcagg cggattgtgt
     4621 attttgggga caattttttc agacggcatt caaggttttt tcctgattgc cgccgcgcct
     4681 aaaaaccgcc tttcgcgctt aatcaaaaat accgacaacg gaatattgcc caaagcgaca
     4741 atcagataca acaaggaaat gctgtcaaac aaaaacagca acaccgcgct caaaacggcg
     4801 gcggaaacca taaaaatacc gttaacgata ttgttggcgg caacggcgcg ggcgcggaaa
     4861 gtctcgctac tggcggtttg cagccaagta tagagcggaa cggagaaaaa tccgccgaaa
     4921 aagccgatca gcgtcatcac cgccatcacg ggatatgccc atccttgcga taaaaaccaa
     4981 aaaatgccgt tcagcccttc aaaacggtgt ccgtgcgtca gccacaccaa aaccaggccg
     5041 caaaccgtca aacccaacgc accaaccgtt acccaagcca acatcagccg ttccctgctg
     5101 aacttggcac acagtaccga accggcggca ataccgatgg aaaacagggc aagcatcagg
     5161 ttgaaaacat tgtcgttgcc gcccaaatgg atttgggtaa aggtcggcag ttgcgtagta
     5221 tagaccgcgc cgacaaacca aaaccacgaa ataccgataa tagcggtaaa gacgggtttg
     5281 taccgcaccg tttcacgaag cagggatttt gtgccacgga caatattcca ctcaatttgg
     5341 gtatcggcag ccttggcggg gacggacggc ataaacaggc tgccgaccgt gcctccgacg
     5401 gcgaccagca aaaccagtat cccgacaata taaggcggta cacctgccac cgccgttccc
     5461 aaaatctgac cgaacaggat ggcgacaaac gtacccgatt caatcaggct gttgcccatc
     5521 atcaactctt tgtcgtcgag ataatcgggc aggatggcgt atttcagcgg cccgaacagc
     5581 gtcgattgcg cgcccatgca aaacagacac gccaaaagca gcggggcaga ccggatataa
     5641 aacccgtatg ccgccaccgc cataatgatc atttccagca ccttgaccca acgcgccaaa
     5701 acggccttgt cgaatttgtt acccaactgc cccgacagcg aggaaaacag gaaatacggc
     5761 aaaataaaca acaacgcgcc caagttcaac atctgtccgg caggcaggaa gccgttttgc
     5821 cccaaaccgt aaaacccaat catcacaaac agcgcggttt tgaacacatt gtcgttgaac
     5881 gcgccgagaa actgcgtagc gaaaagaggt gcgaaacggc ggcttttaac cagtcccaaa
     5941 ccgccttttt tagcgtacat cgttttccct ctctttttca atcagtttac ttgtcgaatc
     6001 atcatccatc aggatgcggt gcgccggccc ttccaagtcg tcaaactgcc cgtttttgcc
     6061 cgaccaccaa aaaaaccagc cgatgacaaa cgccaaaata atgctgatgg gcaccaatat
     6121 aaacatgctt tccatcacat attccctgtc aaatcgttca aaacaaaagt ctgccccgac
     6181 acggtcagat attcgttacg caaagttccg acgggagctt cgtcaaaaaa cagctcgata
     6241 cggtctttga ccacgcgcca atattgggga atttccgtct gaccgaacgg cgacaggaca
     6301 tgattttcca ttccgccttc aagtttgacg gcaaaacgcc cgctttgcgg ccgtgcttcc
     6361 gattcgtcgt cggcaagcag gatgaaaaag cctatatgcc gtcccgattg gtcatgaata
     6421 ctgaaataat gcataaattt cccacccgcc ttttttcaga cgacaccaac taaaaacagg
     6481 gcgaatgtac cagtttggac gggaagaatg caaagaaatt ctccctcccc cagccgaaaa
     6541 caccggcaaa ccgcatatcc cccttttttc cgtcaaaata cccgacttcc gccattttca
     6601 cgcaaacgcc cgattaagcc aagcaattgc aaagattttt tgttagaata gcctgcttct
     6661 tttatcaacc gtttcagacg gccccactac tttcccgcct aggaaggcaa aacggattcg
     6721 gcacgaatcc ggttagtatc cgtgtccgat tccaatgccg tctgaaactt tccggagtaa
     6781 gaaaatgtcc caaaaattga tcttggtttt gaactgcggc agctcgtccc tcaaaggcgc
     6841 ggtcctggat aacggcagcg gcgaagtcct gctcagctgc cttgccgaaa aactcaacct
     6901 gcccgatgcc tacatcacat tcaaagtaaa cggcgaaaaa cacaaagtcg atctgtccgc
     6961 acatcccgac cacaccggcg cggtcgaagc cctgatggaa gaactcaaag cccacggcct
     7021 cgacagccgc atcggcgcca tcggccaccg cgtcgtcagc ggcggcgaac tgtacagcga
     7081 atccatcctc gttgacgacg aagtcattgc cggcatcgaa aaatgcatcc cgctcgcccc
     7141 cctgcacaac cccgcccacc tcttgggcct gcgtgccgcg caaagcattt tcaaaggcct
     7201 gcccaacgtc gtcgtattcg atacctcctt ccaccaaacc atgcccgaag tcgcctacaa
     7261 atacgccgtt ccgcaggagt tgtatgaaaa atacggcctg cgccgttacg gcgcgcacgg
     7321 taccagctac cgcttcgtcg ccgacgaaac cgcgcgcttc ctcggcaaag acaaaaaaga
     7381 cctgcgtatg gtcattgccc acttgggcaa cggcgcgtcc atcaccgccg tcgccaacgg
     7441 cgaatcgcgc gacaccagta tgggcctgac cccgctggaa gggctggtaa tgggtacgcg
     7501 cagcggcgac atcgatcctt ccgtattcgg cttcctcgcc gaaaacgcca atatgaccat
     7561 cgcccaaatc actgaaatgc tgaataaaaa atccggtctg ctcggcattt ccggcctgtc
     7621 caacgactgc cgcaccattg aagaagaagc cgccaagggg cataaaggcg cgaaattggc
     7681 cttggatatg tttatctacc gccttgccaa atacatcggc agtatggcgg ttgccgcagg
     7741 cggtttggac gcactggtct ttaccggcgg catcggcgaa aactccgaca tcatccgcga
     7801 acgcgtgatc ggctacttgg gcttcctcgg tctgaacatc gaccaagaag ccaacctgaa
     7861 ggcacgcttc ggcaacgccg gcgtgattac cactgccggc agcaaagccg ttgccgtggt
     7921 cattccgacc aacgaggaat tgatgattgc acacgatacc gcccgcttga gcggtctgta
     7981 agattcaatc cccacgcgaa ctgcctccga aaacggaggc agttttttta tccggctttc
     8041 catgcttaaa cagcactgcc tcttttcaga cattgacggt tgcagccgct tacctgaacc
     8101 ttatagtgga ttaacaaaaa ccagtacagc gttgcctcgc cttagctcaa agagaacgat
     8161 tctctaaggt gctcaagcac caagtaaatc ggttccgtac tatttgtact gtctgcggct
     8221 tcgtcgcctt gtcctgattt ttgttaatcc actatatccg actttccata cttaaacagc
     8281 actgcctctt ttcagacatt gacggttgca gccgcccacc tgaaccttaa tgattgacta
     8341 ttttttaatc atgttattat tttccataaa atacatggca ttaagatgtt tttccacaaa
     8401 agatacacac accggcaaac accggctgtg tttatctttt cttatgccta ttttttaatc
     8461 atcgtatttt tatcttttaa tttcaatacg caaactaact tatacacacg gttttcacat
     8521 ctttagactg cttccgtgtg tatagtggat attgccgttt tcctttctga caaaaatgcc
     8581 gtctgagaac ttcagacggc atttgaaaca tcggaatcag cggttttgtt cataccactc
     8641 gataaacttg tctgctttga caaaacccag cagcggctcg ctgcggctgc cgtcggagcg
     8701 gacgacaaac acgcccggcg gcccgaacag accgtattct ttcaacaacg cctgatgttc
     8761 aggcgtgttg gcggttacgt cgatttggaa aaagcgttcc atatcaaccg cctgatgcac
     8821 ttccggctga ttgagcgtgt atgccgccat ttctttgcag gaaatacacc agtcggcata
     8881 aaaatccaaa acgacgggtt tgtcgggatg ttctttcaac gccgtatcca tcgctgcctt
     8941 cagcgcggca gtatcggcaa acattttgcc gtgttccgaa gatttgcctg cttcggctgg
     9001 tggattgagg gtcaggaaat ggtgcagcgc ggtcgttttg ccgtttgcgc cctgccagcc
     9061 gaaccacgcg ccgcctatca gcaatatgcc gcccaatgcg aatgccacag ctttcggacg
     9121 gcgtttctgc ctgcgtccgt tgaccagcag catacaggca ggaaccagca tcagcagcgt
     9181 gtacagcgcg acgacgagat aatagggcaa gtgcggcgtg gcgaggtaaa cggcgacggc
     9241 aagcaggata aagccgaaag cgtatttgac ggcattcatc caatcgcctg ccctaggcag
     9301 gatatgcccg ccgaacgtgc cgatggcaat cagcggaacg ccggtgccca acgccaaagt
     9361 gtaaagtgcc aaaccgccta aaaccgcatc gcccgtctga ccgatgtagc ccaaagcaaa
     9421 tgccagcggc ggggcgacgc acggcccgac aatcagcgcg gacaatatgc ccataataaa
     9481 gacggaaacg attttaccgc ctgaaagcct gctgctctgg ttttggaaat acgactgcac
     9541 ggcgttggga agctggatgt tgaacagccc gaacatagac agtgccaaga cgaccattaa
     9601 agccgatgcc gccaacacca cccaagcctg ctgcaaccat acggtcagca gcgcgcccgt
     9661 cagtccggca acaatgccga ccagcgtata agtcagagcc aaaccctgaa cataaacgac
     9721 ggacagcaca aacgcccgcg ccttgcccgc ctttttgtcg ccgaccacaa tactggaaac
     9781 aatcggcaac aggggataca tacaggcggt aaaactcaga cccaaaccgg cgagaaaaaa
     9841 cgccaaaaga ttggcgttga gcgtatccca agacagcttg aaacggctgt cgccgccctc
     9901 atcccccttc gggggcggca acgccccgct gccgttttga gaggaaggct gcaaaaagcg
     9961 gtctttggcg gatgccggtt cgtcggtttg cggatggtaa gtgccgttgc cggaaatatc
    10021 aaactcggta tccacgggcg gatagcacac gccggcttcg gcacagccct gataggtcaa
    10081 aaccaattta tacggttcgc cgacagcctt tgcataagga aaggcaacct gcgcttcgtg
    10141 atggtaaacc gtctgcctgc cgaaaaactc gtcttccttc tcttcgccct tgctgaaaga
    10201 aggctgtccc aacaaatccg ccggatcggt cttgccgacg attttcgcct gatacatata
    10261 gtatccgtcg gcaatcctga aacggacgtt cacaccgtcg tcggcaacgg caagctccgg
    10321 cacgaatgcc ttttccggcg gcagcagatc gttcgcatcc agcgcgaaag ctcgtccgca
    10381 caacatcaaa aatacggcga acaggcaaat cagttttttc ataatcgaat ccgtttcaga
    10441 caaataattt gtctgcatta taaatggtaa ggttgacggt gggatttaat ttatgtaaaa
    10501 cccgccatta tccgaaccta tttccataaa catcttaccg aacccgccat gtacgatgtc
    10561 aatacccacg atgtccgccg ctttttcgcc cgcgtgtggc agcagcggct caatccgctg
    10621 caactgagcg tactggaaca gaaagccctc cgcattatcg aagcccatcc cgaataccac
    10681 cgttatctcg aacgcatcga agaccatctg gacaccgact ggctacccga aaacggcgaa
    10741 agcaacccct tcctgcatat gtcgctgcat ttgtccgtcc aagaacaggc gggcatagac
    10801 cagccgcacg gcatacgcgc aatccacgac accctgtgcg ccaaacgcgg ctggctggaa
    10861 gccgaacacg aaatgatgga ggcactggcg gaaacactgt ggacggcgca acgctacggc
    10921 accggtttgg atgtcaattt ctacatgacc cgactgcgca aactcatcgg cttgggtgca
    10981 gaagatcaag ctaggttgaa cccgcatgaa atcgcctgac cacaccaacc gcctgcaaaa
    11041 tgccatctga agcggaacaa cccctttcag acggcatgca tttcccccat catttccaca
    11101 acgcgcgttt cagcataatc aaccaatcct tcttatccaa aacggggcgt tgtgcaaaca
    11161 catcgtatcg gcacgcgtcc agtttctgca aaatcaactg cgcccccaac acaatcatac
    11221 ggagttccaa accgatacgc cctttcaatt cgcgcgctaa aggcgaaccc gccttcagca
    11281 tacgaaatgc gcgccggcac tcatacgcca tcagccgctg aaacgccgca tccgccctac
    11341 cctccgcaat ctgtccttcg gaaacaccga atttcaacaa atcgtcctgc ggaatataaa
    11401 ccctgccctt ttgccaatcc acagccacat cctgccaaaa attcaccagt tgcaaagccg
    11461 tacagatgcc gtcgctttgc gccacgcaca ccgcatccgt tttcccgtac aaagccagca
    11521 taatgcgtcc gacagggttg gcggaacgcc ggcagtaatc ggccagttcg ccgaaatgcg
    11581 cgtaccgcgt tttaaccacg tcctgcgaaa acgccgaaag caaatcataa aacggctgca
    11641 aatccaaacc gaacggcaca accgcctcgg catccaatcg tgcaatcaaa ggatgcgccg
    11701 accggccgcc cgatgccaac acgtccaact cgcgccgcaa agcatccaac cccgccaacc
    11761 tggcttcaga cggcatactg ccctcgtccg ccatatcgtc cgccgtccgt gcaaacgcat
    11821 acaccgcatg aaccggcttc ctcaacctgc gcggcaaaat cagcgaaccg acgggaaaat
    11881 tttcataatg cccaaccgac ataccttctc catccatcaa acaaaatgcc gtctgaaacg
    11941 gaacaaaccc ttttcagacg gcatcagata cctccaagct gccggcaatc agtggtggtg
    12001 atgaccgtgc gggccgtgga catgaccgtg tgcgatttcc tcatcggatg catcgcgcac
    12061 gctttcaacc gtagccttaa aacggatttt catgcctgcc aaaggatggt tgccgtccac
    12121 caccgccttg ccgtcggcaa catcggttac acgatagacg acaacatcgc cggtttcagg
    12181 atcgtcggct tcaaacatca tgccgacttc gacttcaaca gggaacacgc ccacatcttc
    12241 aatacgaacc aactccgaat cctgctcgcc gaacgcatcg tcgggcgaaa gcgccacatc
    12301 gaccgtatcg ccggcatcct taccgtgcaa cgcctcttcc accaaaggga aaatgccgtc
    12361 gtaaccgccg tgcagatacg caatcggttc ttcggttttg tccaaaagct gattgttggc
    12421 atcatacatc tcataatgca gcgaaaccac ggaattcttc acgatagcca tatttgtcct
    12481 ttcaggaaca gcagattgat tacaggcgca ttctaacaca accgccgcgc cggccgatta
    12541 ccgttaacct gttcataaac tgtacagcac atatttcaat gtaaattttt gttattttat
    12601 tgcgatgtaa cttttttaca acattattaa aaccattccg acctgtctgc cgactttccc
    12661 aatccgcctt aataaatcat acaagatact gaaattatat taatctctat aatatttatc
    12721 cctatcgaat ttttaacagc aaaaccgttt tacaggattt atcaatccgc ccgccagaaa
    12781 acttttcatt caaacctttt tcccatctgt acgacattgc aatcccttat tccatagtgc
    12841 ataattacgc aaattcagcg atgaatttcc aacccggttt gtagtatggt cgataaagac
    12901 ctatttgttt caataattta aattggttct aaaggttact aaaatgaaaa aatccctgtt
    12961 tgccgctgct ttgttgtctt tggtcctggc agcctgcggc ggtgaaaaag ccgctgaagc
    13021 tcccgctacc gaagcacctg ccgccgaagc tcccgctgct gaagctgccg ctactgaagc
    13081 acctgccgcc gaagctccca ctgctgaagc tgccgctact gaagcacctg ccgccgaagc
    13141 tcccgctgct gaagctgccg ctaccgaagc acctgccgct gaagctcctg ctgccgaagc
    13201 tgcaaaataa gcattttccg cttgcaaaaa agcaggatac gttcagtatc ctgctttttt
    13261 gatttttcag acggcatcag attcccttcc tcaatcttct ccctaccctt ccgacaaaca
    13321 tgcctgacct tcataccgaa ttttcccgac tcctgccggc agatgaaatt gccgaacttt
    13381 ctccgacgct tttaaaagac cagcgcaacc gctttacgtc tgcaccagac atcattttgc
    13441 agccgcgcag cgttgaaagc gtgcaaacca ttatgcgttt ttgttacgaa caccgtattc
    13501 cggttacgcc gcaaggcggc aataccggtt tgtgcggcgc ggcagtatcg gaaaacggcg
    13561 tattgctgaa cctttccaaa ctcaaccgca tccgcagcat caatttgtca gacaactgca
    13621 taaccgtcga agcaggttcc gtactccaaa ccgtccaaca ggcagccgaa gcctcaaaca
    13681 ggctgttccc actcagtctc gccagcgaag gctcctgtca aatcggcggc aacatcgcct
    13741 gcaatgccgg aggtttgaac gtattgcgtt acggcacgat gcgcgacctg attatcggtt
    13801 tggaagtcgt cctccccaac ggcgagctgg tttcccatct ccatcccctg cataaaaaca
    13861 ccaccggcta cgacctgcgc catctgttta tcggcagcga aggcacactg ggcatcatca
    13921 ctgccgccac actcaagctt tttgcccgtc cctcggacaa ggcaacggca tgggtcggca
    13981 tacccgacat cgaatccgcc gtccgcctgc tgaccgaaac ccaagctcac tttgccgaac
    14041 gcctatgcag ttttgagctg atcggccgtt ttgccgccga gttgtcttcc gaattcagca
    14101 aattccccct gccgacacat tcagaatggc atattttact tgagttgacc gactcattac
    14161 ccgacagcaa tcttgatgat cggctcgtcg aatttcttta taaaaaagga tttaccgaca
    14221 gcgtattggc acaaagcgaa caagaacgta tccatatgtg ggcattgcgc gaaaacatct
    14281 ccgcctccca acgcaaactg ggcaccagca tcaaacacga tattgccgtt cctatcggac
    14341 gcgttgccga ctttgtccgc cggtgcgcca aagatttgga acagaatttt aaaggcatac
    14401 aaatcgtctg cttcggacat cttggcgacg gcagcctgca ctacaatact ttcctgcccg
    14461 aaatcctcag caatgaagtc tatcgttacg aaaacgacat caacgacgca gtttatcgca
    14521 acgttcttgc ctgcaacggc acgattgccg ccgaacacgg cataggtatc atcaaaaaac
    14581 agtggctgga caaagtacgc acgcctgccg aaatcgccct gatgaaaagc atcaaacaac
    14641 accttgatcc atataacatt atgaatccgg gcaaactgct tccgtaaccg gcatttctga
    14701 tttgcataca caacaaagaa agggacaata gatccgattg tcggtttagc gcgagctcgt
    14761 gagtgcggta aaaaattggt ggaaatgaca cgaaaaatga ccgcattttt aaaataaaaa
    14821 aatcggcagt gaatttccct gccgatttta ttttgttaca acttaactta aaacgtccac
    14881 tgcaaattca acgcaccttg tttagcctga tgatatttgc ttgtttggcg gttgaatgag
    14941 gcttgtaaag ttaagtgaga tttgattttc actgctacac ctaattggct ctcaattgcc
    15001 gtcttattgt ttatcactcg acgctctccg tctatttcca caccgaaagg tttgttgtgg
    15061 taaagcgcgt tcacagcggc gaaaggttca atagcgatat ttttatagag tgaaaattga
    15121 gctttagctt gaacgccaac ccgagtttgt aattggcggg agccaagtaa attcacgtgg
    15181 gcattttcgc tatcgctgaa ttttccgttt acccccaaat aagtcaattg tgcctgtggt
    15241 tgtaggtaaa cacgaaggct gttgcccttt ttagtgaagt gttctgccaa taaagcattg
    15301 taacccgctt caattgaggc ggtaatacct tttgaagtaa aacgttctgt agcatcttca
    15361 gtgttaatgc ggtggcggaa gcgttgatat tgtacccagc tatccgcata cgcacctgtc
    15421 tgtttgtcct gaagctgatg ccaagtggca taaatgcccg ccccaaagcc tttcacattt
    15481 cccgttgtaa gattgtctgt atctggattg cggaaagtgc tgcgttgttc tgcttgtccg
    15541 ctcattaagc caacggaaag ttgattactt tcgttttgcc atgtgaatac ttcgccaccg
    15601 agttgcacac ctttacgata gccttctaca ggtgctgttt tgccttgcac ccattggttg
    15661 gaatgtccgt caatcacacg caaccacaag cctttgcgtg gtaaagtgcg gtcaaaaata
    15721 tcgctgtttt tgtcgttcaa acgcaaggcg aataaggtat tggcggcttg agcctgttgt
    15781 gcataaatcg aaatatcatc gcgttcttgc actttggtaa aaaagccttc tgggcgttgt
    15841 tgtaaagaaa gcgtataaat tcccttttgg tgtttgccag aaagacggaa tgcgtgttta
    15901 tctgctgtgc catttacttt gataatttga tgcccgtcga gactttttaa atcgtctatt
    15961 ggattttcga agatgatgtc ggaagtgcca gttatatttt tttcaaaagt taaaactggt
    16021 gtttcagaga ctttatcttt tagatcgtaa gcaaaacgaa aacgagctcc gccagcataa
    16081 tcttctttta cgagtaaact ttcactttta gtattaaaac ggatgtctgc attcgttgtt
    16141 tttaattccc caacatcaga atcccaacgg ggttcccaga gagaattttc taagcggaat
    16201 tcatccaaac taattgtttg tccgataacg tgtgagttgt ctgtaacctc aatatagtgg
    16261 aatggatcta aaccagaata gagatgctct tgccaactag gaagcttatt aagtgttggg
    16321 cgaagccatt cgatataatt acctacagat aaaatttcgc tgttgatatg gctatttttt
    16381 atttgtggag ctaaggcgaa aatatcgctc tttattaatc ttgggccaga gttttcgaag
    16441 gatggttgtt cacacttatc attattggaa ttcccagtat ttacacagtc cttaaaatat
    16501 tgctggagtg ttttttcttc ttccccattt cttttttcaa ccccgaaacc tcgggcgaag
    16561 ccaacaaggt aatcttcagt gtattccttg tcataaaaag aaatcttttt tgagttgttg
    16621 atgtttttga attggtgtgt tttagagtat agtgcgggaa ctttgagaga gttctgagag
    16681 ctaccattcg tcggttttag aagataagtt tcttgtttcc agtattcgct gcttttatct
    16741 ctaagttttt ttaagcgatc aatttgctca tttgttaatt tagaattgtc gtaaacgtaa
    16801 tcaacagcca aaagcggaga ggtataaaga atagaaaaaa atagacttat aataaatgat
    16861 ttttttaaac ttctgcttgc ttgcttcgag tttcataata aattttcctt tgtcaagtaa
    16921 aaataaatgg ggcgtggatt ttagcataaa actgaacaaa aaatgtcatt tagctcacat
    16981 ttttctctat ttatttctta tttattaaag gtaaacgttt gctttttgct attttgtcaa
    17041 gccagtttga aaatgtgtat aattgccctc gttatttaca aaaatttcag gaaaaatgac
    17101 cgcactttac ccttggctaa tgccaattta tcatcaaatt gctcaaacct ttgacgaagg
    17161 tttggggcat catgctgtgc tgattaaagc ggatgctggt ttaggtgtag aacgtttaca
    17221 catcaggcgg cagccttgcc cataccctct gaagcactgt ttccacaatc agcgcgtatg
    17281 cttaatcaac cgctgtttct cgcgtttcca atccgcctct ttcatactct gacgtttgtc
    17341 gtgctgtttc ttaccttttg ccaaaccgat ttccatcttg atttttccgc gcgaaaaatg
    17401 caaatccagc ggcacgatgg tgtagccggc acgttcggtt ttgccgatta atttgttgat
    17461 ttccgactgg ttcaacaaga gcttgcgcgg acgtacggca tctggtttaa tgtgtgtcga
    17521 ggctgtgggc aaagccgtaa tatggcagcc gaccagataa aacgcgtctt ttttccaata
    17581 gatataactc tctttaagct gtacgcgcgc ggcgcggatt gctttgactt cccagccttc
    17641 caagaccaaa ccggcttcaa tccggtcttc aatgaaaaaa tcgtgaaatg cttttttatt
    17701 gttcgcaata gccataaaca tcctatcaat atccgccgtc agacggcata aacccgaaaa
    17761 cagaacccat cataccgcct cttcaaccgc ctgcacaatc ttctcgggat acagcctgtt
    17821 gaggcagtcg gtatgcccca gcggacattc ccgcttaaaa cagggcgaac attccaagtg
    17881 caggctgacg attttcgccc tgtcgctcaa aggcggcgta tgcgtcgggc tggaagaacc
    17941 gtaaaccgcc accaccttcc tgcccaatgc cgccgccaag tgcatcagcc cgctgtcgtt
    18001 gcacacaacc gtgtccgcca acgacagcaa atccattgcc tgcgacaaat cggttttgcc
    18061 gcacaaattg acacacatac cgtctgaaag gcagttgatt tcctcggcaa tttcatcatc
    18121 tttttgcgaa ccgaacagcc aaacctgcca acccgccgcc agataatgtt tgcccaactc
    18181 ggcaaaatgc cttgtcggcc aacgctttgc cggcccgaat tccgcgcccg ggcaaaatgc
    18241 caaaacaggc tttccaagat tcaaaccgaa agtttcgaca gaaatttccc gccgccgttc
    18301 atcaatggaa aactcgggga atcccgaatg cccgtcaaaa tcttcctgac tcggatgcgc
    18361 gagagccgta tatctgtcca ccatcaaagg cagacgttcc ttatccagcc tgcgtatatc
    18421 gttcaacaga aaataacggc tttcaccgac ataacccgtc cttttggcaa tacccgttgc
    18481 tagcgcgatg actgccgatt tcaaagaacc gggcaacacg ataacctgat cgtatccgcg
    18541 ccgccccaaa tccctgccga cccgccagcg gcgtttcaac tccaacgcac cgtgtccgaa
    18601 cggattctca agaatttcat tcacttccgg catacgctcg aacaccgcca tcgaccactt
    18661 cggcgcgaac acatcaatcg tgcaaccggg gtgaagttcc ttcaaacggc ggaacaaggg
    18721 ctgggtcatc acgcagtcgc ctatccaact gggagaaata atcaggattt tgatggacat
    18781 aacaagaaac cgaaatcaga caggcagaat tttaccgcga aaccgttgga aaacctatct
    18841 tgtctcattc cgaacgccgt acgtgcaaat atgaaaaagc ccgaacattc aagttcgggc
    18901 ttcaaaattc tggctccccg acctgggctc gaaccaggga cctgcggatt aacagtccgt
    18961 cgctctaccg actgagctat cggggaatgg ggcgtattat agcgtccgga aaaaatgtgt
    19021 caatccttaa ttttggaaaa atgggcgaca aaacgacaag catatgaatc agaaagacat
    19081 taagaccgat gccttaaaag gattgccgtt gtatgaattt ccacagccgt catcacacca
    19141 tatttaagcc cgatgagccg ttctgccctc ccccgcttaa aacaatgccg tctgaacttc
    19201 gccgcgttcc aaagccagta aaaactgttt gcggttcaaa ccgcctgcgt agccggtcag
    19261 tttgccgtcg ctgccaatga cgcggtggca gggaatcagg acagacacct tgttctgccc
    19321 gttggcggcg gcaacggcgc ggacggcttt ggggttgccc aaatgctgcg cctgttcctt
    19381 gtagctgcgc gtttcaccgt aaggaatcgc caaaagcgcg tcccacgcct gtttttgaaa
    19441 cgccgtacca atcatttcca aaggcgtggc aaaggttttc agacggcatt ggaaatacaa
    19501 atccaattcc tgccgcaaaa gttgcgtccg ctcatcctcc cgaaacacaa accgtccgcg
    19561 caaggctttt tggacggcgg caatttcctg ttccaaatgc ttctgcccga caaattccag
    19621 caaacacaag cctttaccgc cgaacaccgc cagcatctcg cccaaaggcg tggcaatggc
    19681 ggcacacacc agctcgttca agctgtcggg ataacgcact tccaacagac ggatggctcg
    19741 gcggatacgg acatattctt caggcgcgca gccgatattg tcccaaaaat cccgatcgaa
    19801 ctgcccggct tcgcattccg tcagattggg atgcggcata acgccgcact caaaaacccg
    19861 ggtttcgagg caatggcgga tttcatccca ttttgacggc agattgttta aggaaggcag
    19921 ggtaatcatt cgtttgctcc gtatctctat catggatttg gcggcaaaat ccccaattct
    19981 cgtcattccc gcacgccgat gcaggaacgg gctatgacgt aaatcttgag ggttgggttg
    20041 cggcaatgcc taaatatgcg atatttctaa agcctcaggg aaaggaatta aagacttcag
    20101 acgacctgca aagggtcgtc tgaaaccacg atttttgcat ttacgcattc cggcacatca
    20161 tccaaccgtt tcggcacatt cctgccgccg ttgacagcct ataatgaatc cacttattca
    20221 tcaagcaaag gaatcatcta tgcaaacccg catcctctcc gccgtactgc tggctttttc
    20281 aaccgctgcc tttgccgggg gcgcattcac gctgcaattc gacaacccgt ccgaagacgg
    20341 cggcttcacg caaaaccagc ttttgagtgc gccttacggc ttcggctgtt caggcgacaa
    20401 tgctccgccc gcgctgtcgt ggaaaaatcc gcccgccggg acaaaaagtt tcgtcctgac
    20461 cgtttacgat aaagacgcgc cgaccggact gggctggatg caccgggtgg tcgccgacat
    20521 tcccgccgat gtccgccgcc gcaatgcggc ctcgctgcaa ttaagccgct gcgccagcat
    20581 cgccgacgac ccgtccgcag ccatatcggc ggtaatcagt ttgcggattt gccgcatcag
    20641 gttgacgcct tcgtacacgg caaaaccgat gccgtcatgc tgcaaccacg ccaacacgcc
    20701 gcaaagcgcg gcctccgccg cattgtgcgg cacttcttca tccgtcagca ccgccgccgc
    20761 ataatcaaac gccgcgccca tccgcccagg gtgcggcagc ttcaccgcat cgcacactgc
    20821 ctgcgccgtc ccgtattgtg cggcaaacct ttctacggtt tcctgttcga aagcaatcca
    20881 ttgcgcctga tagaggccgt ctgaatcggg aatattaata acgtcaaacg tctgtccgcc
    20941 tgccaacgca accgcctcac ccgccgcaat cctcaattcg cgcctcgccc accgcagctt
    21001 cttgcttccg cgccgcacga taagcacagc tggttcatat accgccacgc tgcggtacaa
    21061 ggcggtatga tgttgcacga tgccgcccaa agcacccaat cgttcgcgcg tatgaaagta
    21121 atttgacggc actattatct ccaacacgca gggccgcaca taaggcgcag ccctgtttac
    21181 cctgatttgg aaggggttac ttcccaaaaa taaaccaaaa tcccactgcc ccgaatggcc
    21241 gagtttcaac catattccgt cggtgtgcct gcaacttgac tccctcccct gtgggggagg
    21301 gttggggaga gggtaaaacg gggcagatac agacaatatt tccgttaccg ccccgatgcc
    21361 ctctccctaa ccctctccca cgggagaggg aatggattgc cgttgaaata aatcgctcta
    21421 cataaaaaat caatgtgtta tctcaaaccc acgttaggtc taagcaaatg gtcggacatc
    21481 cgtatccggc aagcaagctg ctttcagacg gcatttccag ccaacaagcg cgccaatatc
    21541 ccctcataca ccgcagacag cttcggaata tcgtccagcc gcacgttttc gttgatttgg
    21601 tggatggtgg cattggacgg gcctaattcg atgagttctt ttgcaatggc tttgatgaag
    21661 cgtccgtccg aagtgccgcc ggtggtggac aattcggcct caatgccgca ggtttcggca
    21721 atggctgcgc gtgccacgtc ggtcagtttg cccgcttggg tcagaaaggg ctgccccgaa
    21781 cacgaccact gcaaatcgta ttgcacgccg tgtttgtcca aaatggcgtg gacgcgttgt
    21841 ttcagccctg cttcggtgga ctcggtggag aagcggaaat tgaatttgac gttcaactcg
    21901 cccggaatga cgttggtcgc gcctgtgccg ccgttgatat tggaaatttg aaagctggtt
    21961 ggcgggaaat attcgttgcc ttcgtcccag acttcctgcg tcagctctaa caaggccggg
    22021 gcaaaagtat gcacgggatt gattgccaaa tgcggatagg caatatggcc ttgctt
//
LOCUS       DAWHXZ010000006        21746 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000006 DAWHXZ010000000
VERSION     DAWHXZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21746
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.006"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..92)
                     /locus_tag="WIE35_000156"
     CDS             complement(<1..92)
                     /locus_tag="WIE35_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ4153143.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSD"
     gene            complement(82..1413)
                     /locus_tag="WIE35_000157"
     CDS             complement(82..1413)
                     /locus_tag="WIE35_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ4153144.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAVAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAAINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDMARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(1413..3584)
                     /locus_tag="WIE35_000158"
     CDS             complement(1413..3584)
                     /locus_tag="WIE35_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ4153145.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPYTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALVWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            3739..4321
                     /locus_tag="WIE35_000159"
                     /pseudo
     CDS             3739..4321
                     /locus_tag="WIE35_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4365..4634)
                     /locus_tag="WIE35_000160"
     CDS             complement(4365..4634)
                     /locus_tag="WIE35_000160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153146.1"
                     /translation="MPKTVRRYGEPRRAVGGGAVKRSYLTAHKSYRQAVILMAKAGIG
                     ADAVRDMCHAELSAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(4574..4939)
                     /locus_tag="WIE35_000161"
     CDS             complement(4574..4939)
                     /locus_tag="WIE35_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153147.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     CLKPSAATASPDAPSAAEQ"
     gene            complement(4943..5320)
                     /locus_tag="WIE35_000162"
     CDS             complement(4943..5320)
                     /locus_tag="WIE35_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ4153148.1"
                     /translation="MSDATYTGAVIMEVNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEGRRTSYLDCFTVDTS
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(5385..6794)
                     /locus_tag="WIE35_000163"
     CDS             complement(5385..6794)
                     /locus_tag="WIE35_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ4153149.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVAGRLKTAITAADVTVTAS
                     GSGVAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANVFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVFNAIEQRGCIGVLGMSAALSTATTATGKINDGRI
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPWFAECNNA
                     LYNGLTPLTAVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(6797..6994)
                     /locus_tag="WIE35_000164"
     CDS             complement(6797..6994)
                     /locus_tag="WIE35_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ4153150.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(6991..7641)
                     /locus_tag="WIE35_000165"
     CDS             complement(6991..7641)
                     /locus_tag="WIE35_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ4153151.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(7631..8053)
                     /locus_tag="WIE35_000166"
     CDS             complement(7631..8053)
                     /locus_tag="WIE35_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ4153152.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(8053..8451)
                     /locus_tag="WIE35_000167"
     CDS             complement(8053..8451)
                     /locus_tag="WIE35_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153153.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
     gene            complement(8516..9424)
                     /locus_tag="WIE35_000168"
     CDS             complement(8516..9424)
                     /locus_tag="WIE35_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ4153154.1"
                     /translation="MDKSAILTAITAAFRKEFQTGLESVKPDYTAVAMTIPSNTATNT
                     YAWLGKFPQMREWVGSRQIEKMNKQAMSLDNKKFEATVGVARTDIEDDQVGMYRPMMA
                     AMGESAAALPDTLVWGLLKKGKTTVGYDGQYFFDTDHPVYEKSDGTGQNTPHSNLTTG
                     TDNDAPTFYVVDDTKTLKPLIFQNRTDSEFETKFDPSKSDRVFMEDEYLYGSRRRCNA
                     GFGLWQLMHMAEKTALTRENLAAIIVKMQKIKADGGYVLNVKPSLLVVPPELEDKARE
                     LLEADKINGTTNTFKGRLKLHVCVHL"
     gene            complement(9424..10485)
                     /locus_tag="WIE35_000169"
     CDS             complement(9424..10485)
                     /locus_tag="WIE35_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153155.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKSETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAEGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            complement(10716..11132)
                     /locus_tag="WIE35_000170"
     CDS             complement(10716..11132)
                     /locus_tag="WIE35_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ4153156.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            complement(11260..12585)
                     /locus_tag="WIE35_000171"
     CDS             complement(11260..12585)
                     /locus_tag="WIE35_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ4153157.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLGNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQSFGTDLYAMLPDLIHEPEYIFYDKSRGKESFVLLGK
                     PLEGNEKYRLMAVLKYLPQYDSIFLDSFRRADEKEIVKSKKRYEMKKGY"
     gene            complement(12572..14140)
                     /locus_tag="WIE35_000172"
     CDS             complement(12572..14140)
                     /locus_tag="WIE35_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ4153158.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            complement(14140..15762)
                     /gene="terL"
                     /locus_tag="WIE35_000173"
     CDS             complement(14140..15762)
                     /gene="terL"
                     /locus_tag="WIE35_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ4153159.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDALPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIATANDVKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWRGVKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            15774..16286
                     /locus_tag="WIE35_000174"
     CDS             15774..16286
                     /locus_tag="WIE35_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153160.1"
                     /translation="MTGFISNAFIGLKEAGSLLKGFNQLKTEAEVAQKTIELNQIIAG
                     IQTDLFEAQSSYTAAISRIDELEKELVAVKNWSTEKQRYQLYELAPGSFVYRLKTEMA
                     NSEPIHDICPQCYEQGVKSILQFNGYEGAFHKYSCSRCQTIILGERLKSDVEVFSVGG
                     SRYGRLIDGY"
     gene            complement(16283..16789)
                     /locus_tag="WIE35_000175"
     CDS             complement(16283..16789)
                     /locus_tag="WIE35_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ4153161.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     AEVEKKFG"
     gene            complement(16792..16989)
                     /locus_tag="WIE35_000176"
     CDS             complement(16792..16989)
                     /locus_tag="WIE35_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153162.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(16986..17252)
                     /locus_tag="WIE35_000177"
     CDS             complement(16986..17252)
                     /locus_tag="WIE35_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153163.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            complement(17264..17602)
                     /locus_tag="WIE35_000178"
     CDS             complement(17264..17602)
                     /locus_tag="WIE35_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153164.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            complement(17599..17817)
                     /locus_tag="WIE35_000179"
     CDS             complement(17599..17817)
                     /locus_tag="WIE35_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153165.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            17830..18000
                     /locus_tag="WIE35_000180"
     CDS             17830..18000
                     /locus_tag="WIE35_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ4153166.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            complement(18045..18350)
                     /locus_tag="WIE35_000181"
     CDS             complement(18045..18350)
                     /locus_tag="WIE35_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153167.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTSAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(18247..18666)
                     /locus_tag="WIE35_000182"
     CDS             complement(18247..18666)
                     /locus_tag="WIE35_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153168.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKSAFAKQQVVIE
                     KMKRDKAQAMLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYKRALGYGN"
     gene            complement(18638..18874)
                     /locus_tag="WIE35_000183"
     CDS             complement(18638..18874)
                     /locus_tag="WIE35_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153169.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETASE"
     gene            complement(18877..19041)
                     /locus_tag="WIE35_000184"
     CDS             complement(18877..19041)
                     /locus_tag="WIE35_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153170.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            complement(19044..19250)
                     /locus_tag="WIE35_000185"
     CDS             complement(19044..19250)
                     /locus_tag="WIE35_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153171.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            complement(19247..19789)
                     /locus_tag="WIE35_000186"
     CDS             complement(19247..19789)
                     /locus_tag="WIE35_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ4153172.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNSAAGKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGHHSRLAYAQAGGIVEPFFISNDTDLALFKTTK
                     WGICRAIADAIAMKLGAARV"
     gene            complement(20097..20525)
                     /locus_tag="WIE35_000187"
     CDS             complement(20097..20525)
                     /locus_tag="WIE35_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ4153173.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(20506..20940)
                     /locus_tag="WIE35_000188"
     CDS             complement(20506..20940)
                     /locus_tag="WIE35_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ4153174.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
     gene            complement(21097..>21746)
                     /locus_tag="WIE35_000189"
     CDS             complement(21097..>21746)
                     /locus_tag="WIE35_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ4153175.1"
                     /translation="QMTAQKEDSQTMPYQPPTEAEEVLEALVNRRNQPADMRTAEKNR
                     LHQVHETQVGSVKQLIAHFDRLIDELDKQIDNHTHTHFDGKDQVAEQIKGIGSITTAT
                     LMAMLPELGRLSHKRIAGLAGIAPHPREGGETKFKSRCFGGRSAVRKALYMATVAATR
                     FEPLIRDFHQRPLSEGKPYKVAVTACMRKLLTISNARMRDYFAENDTAENGIRTA"
BASE COUNT         4728 a         6141 c         5298 g         5579 t
ORIGIN      
        1 aagtcgctgt cgatgtcgta gcgctcccag tggcggtgct ctttaccgcc cacgcgcacc
       61 gacacggcat agccgtatga attattttgc atagctgttg accaaagtgc cgcgcttgat
      121 aaacgcgggg tggtggatat ggggattgag ccgcaccagc tctgctgcgc gggccatatc
      181 gccgtaaaac tcgtgggcga tttggtgtat cgtaccgtcg attggggctt ggcgcacaat
      241 cagcggcggc ttttggttga tggccgccgc aaccaacgca ttcagacggc ctgccgccgc
      301 gcgcagggat tctgccgttt ggtaagcctc ggtatacacg gcgttggccg tcagcccacc
      361 agactcggca gcagccgtct gcaccgcccg caaggcggca atctcggcct gcatacggcg
      421 gcgcatggcg cggttaacct caatcaaatc gggcgcggtc atctcttcgc catgcgcctc
      481 gattaatgcc gttgccaccg acaacagcga tgacgtggat aacaggcgca ccgcctgcgc
      541 taccggctgc acctgtttgg cggttaaccg gttcaggcgg ttttgcaggc cgtctgaaaa
      601 gcggccggtc agtaaattat cagggatagc ggcggcacgg tcggcaacag ccacagcccc
      661 gtcaaaccgc tgtcgcggcg accaaccggc atggtgcatg gcattatcgg ccaaacccgc
      721 ctcacggcgt atgccagtat ctaccatgac cgatatatcc gcaaacagct tggccgagcc
      781 gtttttaaac gccgctgcgc tgtatccgcc ccgatcggga aaggcaattt tgtccaaatc
      841 aaacaaacgg cgcaccgcac caaacgtacc cgatgccgca ctccaaatgc ccagcgcgct
      901 gccccataaa gccgatacgc ccgcatccac cgccaacacc gcatcaacaa agccgatagc
      961 cgcctcgcgg taggtgtcga tatcagcaat caacgcctca agctcgacca aaaaggcgtt
     1021 ttcaaaaaca aagatttcct gcgcttcggc cgcctcgcgg aaagtaatat cgatgcccgc
     1081 ataatccaca taatcggcct catgtcggta actccatgat gccgcaatca tgttgtgcat
     1141 ccgcccccaa acagggtgca ccagcacgcc gccgcccggc tgctccagcg catccagcag
     1201 ctttttcaga cggcctgcat agcccttgcc ccaaaacacc gcattaatct gcacctgccg
     1261 cccggtcatg cccatgtctt caaggtcgat accctgcaca aacggccgcg catgctcggc
     1321 caatgccttg ccgttgctct cgtccaccac ctcaatatca aagccgacgc ccttgtaaga
     1381 tgcgtcctgc aataaggtat gccatccgct cattattgac ccgctccacg gccgaacatg
     1441 gccacttgat accgcgatac ctcattcgcg ataacacgtc cgtcgatatt caggctcaca
     1501 ttggtattga tggtctgact tgccgacgcc atcgcacttt gcacttgtgc caaacctgtt
     1561 gtaactgcag ccgtatcctg cgccaatgct gcctgatacg ccgccgtctg ctgctgaatg
     1621 gctgcctgat acgccgccgt ctgagttgaa aacaacggag acaacatctt atcggtaggc
     1681 tgcgctgacg gagcagccaa atactgaggg acaccacccc gcgcctgttc ccgcttcggc
     1741 gcaggttcaa atcggatacc gttgtttttc agacgattaa tcatttcagg agttgtcttg
     1801 gtaccacgca aaccctttgg caatgtgccg tcacccaaag actcggaata cagcaacata
     1861 cctaacaaac ccgcccgttt aactaacgct ggattattca tgagcccgct accggcagat
     1921 tttccccatg acagcaactt accgcctgcc gctgtggcac ctgctgccac gccgcctgcg
     1981 gaagccttac cccataccaa cgctttacta cctacatctt tcagaaagcc tgcaccttta
     2041 ccgccaccgg cggttaataa cattgcggca gacgccgctg ttgccgccgt cgtcaatgct
     2101 tgcagggcta gagtggcatt agggtattcc gctgccagct ttttaaacga ggtttccata
     2161 tccaccaatg ttcccgtcag actttcccgc aacattgcca gcgattcctg ttgctcttgt
     2221 tgcgccgctg ccgacaacat tcgcgcctcg ttgttcttag caatttttcc gttatccaac
     2281 gttacgccag ccaacgaagc catatattca cggatttgcg tcatatccgt tgcagccagc
     2341 aaaccttgtt ttgcctgcaa atcaggcagc agttgcgcca aaagcgcgcc cttaagcata
     2401 tttgcctgct ccgccgccgt cttatcgcct gcagccgcgc gtttcttata atcttggtat
     2461 tgcttatcct ttactagcat ggcatcggca agacgggaca acacctgcac tgcgttttcg
     2521 ccgttttgct tgccttgcac aaccgagcct atccaatcga cacctttctt cgggtcattt
     2581 ggatttgcca tcttcttcag acgacctatc gtgtaaggcg acagagtttt actcaaaaga
     2641 ttttgcacat tagtcgccgc ttcggcagga ctgcccgatt tattcgccgc agattgtaag
     2701 agtgagagca ggtagtccaa accgccgaca ccattcatcc ctgcctgttg cgcggcaggg
     2761 agcaggctcg gcagctcccg aaccatatcc cgcacctcga aagtgccgtc taaacccgat
     2821 tgcaagacgt gctcaagccc gagttgcagg tctttaccgc tcatgccgcc atctttcagg
     2881 gttttaatca gttttgccgt atcctcgcca ctgccttctg aggcaagtgc aaaagcatat
     2941 gccgcctgcg cttcattctt ggtttgggca aaattcagac tggtggtcat catgccgctg
     3001 attaaatcca aagccttatc gtgggtcccg ccatttttct cgacaagttc aagtgccaaa
     3061 tccttgatct gttgcgcacc ttcagttgca atccacgctg ccgatttact gttatcctca
     3121 ataaatgcct gtctggacac gcggttgatg ttctcatcaa gctgctttct gttgtccata
     3181 gcaggcttaa gcaccgtata cgctgccgcg ccaccagcca ccgcagctgc accgaatctt
     3241 ccgatttttc ccatcttgtc cgcaaatccc gtgccctgtt tcagttccgc gttcagctcg
     3301 cgaattcggt tacgcgtagc gaccgccgcc cgtgccaaat cattttgtga tgccttgccg
     3361 ctgcgcgcca aacggttaaa cgcagcctgt gtcagcatga tttcgcgttg aatctgtttt
     3421 tctgagcgga tgccggtcaa cgcataagta tggctttgcc gttcaagttg cgcccgcgat
     3481 ttcgcggtac gatctaattg tcgttgagta tcagccagta gccgtctcgc tccgtcatct
     3541 cgggcggtta acaccaacga cagtttcatg ttcccgtttg ccataaaaag cagcctttaa
     3601 acgtattaac tcaaggtcaa ttatcgttta aaggctgttt aagggaggcg gtatgggctt
     3661 caaccccgtt ttcgtgcttt atttgtttgt tgcgttataa taccagaatg ataaccattt
     3721 tattcaaagg gatattttat gaatatgaaa aaattgattt ccgcaatttg tgtttcaatt
     3781 gttttatcag cctgcaacca acaatcaaaa acggcacaag ccgaagaacc tgtccaaagt
     3841 atccaggctg ctgattgtac cgccccaatg gacatcacag ttgaacaata tctcatcaat
     3901 ttggagcaag catttaaaac tcagaacgtc tcaacaaaaa tccataataa aaatattgtc
     3961 aagaccgatt gtggttatga ccttactttg gtaatggatt ttggggcgat tgcgctcaaa
     4021 ctggacgagc agcaaaaaat tagagctatc tcagtaggct acattttaaa aaccgacgga
     4081 gagaaaggac aaaatctagt caataatgcc ataaatggat tacacagtat tcaggcagtt
     4141 ctgtctttaa ctaccacaga caaattgggc gaatcggaag caggaaaaca acttcgtcaa
     4201 agaatccaat cagacaggag caacagcgca aaaagacgtt ccggcagatg gtattttata
     4261 tagcgttgtt tttgaaaaag aaacaaacac cattgcaata atcggcagaa aacaacccta
     4321 accgtcaaat aaaaaaaagg tcgtctgaaa ctcagacgac ctttttattt tccctccggc
     4381 tttctcagcc gtttcgacac aatcactccc tcttccttcg gcgtttttat gcccaggctc
     4441 gacaggatgt cttcaaacca tgccgacagc tcggcgtggc acatatcgcg cactgcgtcc
     4501 gcccctatcc ccgcctttgc catcaggatg accgcctgac ggtagctttt gtgtgccgtc
     4561 aggtagctgc gttttactgc tccgccgccg acggcgcgtc ggggctcgcc gtagcggcgg
     4621 acggttttag gcacaaatcc tcctgagccg ccatcacggt atggtagtct tcggtagaga
     4681 ggttttccat cagccactgc gccgtcagga tgtcggatgg gataccttgg gcttcaatct
     4741 gctgcgacca gtaggtcagc gtctctagga cgtcgcgttg cagcagttcg gcttttgtgg
     4801 cgttttcggg cagctcgggc aagtcatcaa tcagggcgaa cgccgccagt tcgccgccga
     4861 cggtcagtgg cttgaggcgt aattcagata cgactttgcc attgtaggtc agcccgtggg
     4921 tcagtttgac ggtaatcacg cattactcct tgattttgtg caaagcaatc atctcaatgt
     4981 cgatgcgtgc ctcgttatcg acttcatatt gctcgctggt atcgacggta aagcagtcga
     5041 ggtaggaagt gcggcgacct tcgtcgttga tggggtaaat cgtgattttc gccttggtga
     5101 tgttgtccca gtcgatttcc gtaccgtcga tcggaatggc ggcggtaacg cttaatttgt
     5161 gttcggttac gccgtcacaa taaccgttga ctcggccgtt gcggttcatc gttttgaccg
     5221 gcttgcggcc tgtagtggtt tgcggtttga tgctcacaat ctcgatatcg cggccgttca
     5281 cctccataat caccgcgccg gtataggtag cgtcgctcat gctttatcct tttttaaggt
     5341 ttcagacggc ctttaaacct gttttaaacg ccgtctgaaa agggttacaa aatcaaatca
     5401 atgcgcccgg caaagacgtg caggccgttg accacatcgg cggggataat ggcattaaca
     5461 cggttggggt cgttttgcgc acgcgccacc accagcttgc ctttgttggc ctcggcgttt
     5521 tcgatgattt cggcttggtc gagcttaatc agcacgtcca aaatctcgct cttaaccttg
     5581 ggcagcaggc ggtcgctcaa tttgtcgcgc ggaaaacgca gggcaatgcg ctctttaacg
     5641 ctgcggcgca cataatccag cgtgcgaatg gtggtaatgt cgagtagtgc cgggtcgtcg
     5701 gtgttgttgg ccgacttggt ataggttgat acggcacgca taatctgcac gcggttgttg
     5761 accgctgtga gcggggtcaa gccgttgtac agcgcattgt tgcattctgc aaaccacggc
     5821 cattgcgcat caggtgtaac ggccagccct ttgatttcca gcgtgttcag cggcttggca
     5881 gggtcttctt caaaggccaa caccgccgca taacctgcgg cgatgatgcc gtttggctct
     5941 accgcacctt tgtaccaagc acaggtaatg cggccgtcgt tgattttgcc ggtagcggtg
     6001 gtggccgtgc tcaaggccgc actcataccc aatacgccga tacagccgcg ctgctcgatg
     6061 gcgttgaata cgttggtaat atggttgctc aaggctttgg cgttggcgtc atcgctaaac
     6121 gggctgcaaa taatgtgata atgcttgccc gccactttgg acaaggccgt ggcaatgtcc
     6181 gcatttttgg caccgccggt aaagacattg gcttgataag tcaggccggt attgccggtg
     6241 ctcacggtta agccgctctc gttgccgatc tcgcctttgt gtttggccgt cagcgtaacg
     6301 gctacgccgc tgccggatgc ggtaacggtt acatcggcgg cagtgatggc ggttttcaga
     6361 cggcctgcca ctgtggcggc ggtctcgccg gtgttaacgg ccgtgcttac ctgcttgccg
     6421 ccaatcgtga tttccaccac gcccggcgcg gtggccgtgc cggaaagggt aacggttgcg
     6481 gttgcctgca cgcctgcgct gtggtcggca ataccgataa cggtcaaatc caaataaggg
     6541 ttgttggcaa atgcttggcg caccatcaaa tgcgccagcg agccttgtcc gaacaaatcg
     6601 gccgcctcgg catcgctaaa tagttgcacc ggctctaagg cgggctgtat gcccgcggtc
     6661 agcatgggtg caaccatcaa taccttttgc ggattttgcg gcaaaccttg cacggcattg
     6721 cgggtgttaa attcaatata ctgcccgggc acgcggatgc tgcccggaat cgtgtcaaaa
     6781 tcaatatggg gcatcattac tctcccttag tattgcgggt tttggtattt ggggcggcat
     6841 cctcaaccac caccaaatcg ccgtcatcaa tcatgcggcg gtaatacagg ctgttgccgt
     6901 ccacctccac cggctcttgg ccgatatatt cgtgcgggtt gtgttcggtc ggcacacgca
     6961 ggccatctgc cgccgttact ttgattttgc tcattgctta tccttaaggg ttaggtttac
     7021 aggtatttca tcggcggatt gcgggtcgta aattttgccg tccaacccct cgaaatcagg
     7081 ccacggctcg ctcaatgtac cctgatactt ggtaaagata tggttggggt cgtcgggatt
     7141 gtcggtgcgt tcggggtagc ggtcattttc caacccgcag gtgttaaagc ggatggcata
     7201 ctcaaccgca tatatgctta ctgcggcgtt ttgcaccagc acatgattgg caatcgcgcg
     7261 caccgctttg ggcaccaagc cgcggctatc ggcaaaaccg agccgctggc cgtcaagcag
     7321 gcggcgaaca gcgcggatta aatcgttgct gccgatttcg cggctgtcga tgccgccttg
     7381 ccgctgcgcc tgctcgttgc gcagattgcg ggccgccacc atcaccacaa attcggcggt
     7441 atcctgataa cgtccgcata cgccgccggt gctggcaggc tcaactttgc tgccgccata
     7501 cgttacccaa accgcaggca gcgtatggat ttgccccgcc aaatcgtcgg cctcgccgtt
     7561 gtagctttta accgtgcgca ccatgcgacc caagccccgt ttcagacggt ctgttatcgc
     7621 ttgctcaata tcagtgatca cgcccgaaca ccttattttt accgtttaca aacacaatac
     7681 cgtcttcgcc cgcggccact tcttcgccgc tatcatccac gcccagctgc acttcgcctt
     7741 tggccatagc acgcagcagg tcgagcacgt cgattttgta gcggttgcgg atttcgtcgg
     7801 taatcaacac gccctgagcc gccgtcagac ggtagcgggc aatgtcgcag caaaggcgca
     7861 ccaagatggg cggcagatcc tcaaaaggtc gtctgaaccg ccccagatac gcgtcgattt
     7921 cggcagtggc gtccaccagc gcggtttgtg cgacctcgcg gtcaatcagc ccctcgttgt
     7981 tgcggtcggt gagctgcaag acttccagct caccgaaacg cgcaaccata tcctcaaccg
     8041 tcgcgtatgc cattactcga ccgccttgcg ttgcagcata ggctcggcgc agattgcctt
     8101 ccacaccgct tcgccgactt cggcgcgctt cacttcacgc cagccgccgt caaacagcag
     8161 gccgccgcgc caaaattctt tgccgtctgc gccggtactg acgagcatca catcgcggct
     8221 gtccgccaaa gcgtcggcgg cgcgttgcgt atgctgcact ttgagttcgg caagttcggc
     8281 ggacagtgcc tttttgtcgt cttcggcttt ttccaaggct gtggtcagca tttcgacatc
     8341 gtttcgggcg gcggcaagct ctgcctgcac ggcgtccaat tcggctttga tgtcttcaaa
     8401 cgacggggcg gcggtttcgg cggtttctgg tttgttgttg gtttttgcca tgatgactcc
     8461 ttgtttcaga cggcctttaa ataggtgtta aaggtcgtct gaaaacggtt cggggttaca
     8521 ggtggacgca aacgtgcagt ttcagacggc ctttgaaggt attggtcgtg ccgttgattt
     8581 tgtcggcttc caataattca cgggctttgt cttccagctc gggcggaacg accaagaggc
     8641 tgggcttcac attcaacaca tagccaccgt cggctttgat tttctgcatt ttgacgataa
     8701 tggcggcgag gttttcgcgg gtcagcgcgg ttttttccgc catgtgcatc agttgccaca
     8761 aaccgaaacc ggcattgcag cggcggcgcg atccgtacag gtattcgtct tccataaaca
     8821 cgcggtcgga tttggacggg tcgaatttgg tttcaaactc ggagtcggtg cggttttgga
     8881 aaatcagcgg cttcagggtc ttggtgtcat cgacgacgta gaaggtcggc gcgtcgttgt
     8941 ccgtaccggt cgtcagattg gagtgcggcg tattctgacc cgtgccgtcc gatttctcgt
     9001 aaacggggtg gtcggtatcg aagaaatatt ggccgtcata accgacagtg gttttacctt
     9061 ttttgagcag accccacacc agcgtatcgg gcagggcggc ggcggactcg cccattgccg
     9121 ccatcatcgg gcggtacatg ccgacttggt cgtcttcgat gtccgtacgc gctacgccga
     9181 cggttgcttc aaactttttg ttgtcgaggc tcatcgcctg cttgttcatt ttttcgattt
     9241 ggcgcgagcc gacccattcg cgcatttggg ggaatttgcc gagccacgca taagtattgg
     9301 ttgccgtatt ggacggaatg gtcatcgcaa cggcggtata gtcgggcttg acggattcca
     9361 aacccgtttg aaattctttg cggaatgctg ccgtgattgc ggtcaaaatc gctgatttgt
     9421 ccattactta ccttcgcttt ctttgatttt tacaaattct tcgccggaca tgcccagcat
     9481 ttttgctgcg gctgcctctt ccgcagtcag tgcggcgacg cgttcgtcgg gtgctttacc
     9541 gcccgtttgc gagcctgcca gtgcagcgac cggctgggcg ttttcaataa agccggtcaa
     9601 aaatgccaag ccgcccggct gtttcaatac gccttctgcc cactccttct gagcaggcag
     9661 caatttgcct gaagtcagcg cggcggtaat caattcgttg cctttgtctg cttcctgctt
     9721 ggcagtcagc gcggcgactt tgctttgcag ctcttgaacc actgagatag gcgcgtattt
     9781 agtcaaatca ggcttggcgg tttgcgccgt caatgccgcg atgcggctgt ctttttccgc
     9841 cagctgcgcg aacacgtcgg cagacaatgc cacgtctttc ggcttggctt ccacgagcgc
     9901 ggacaatgcc gccttcagtt cttcttcgcc cgcatcaggc agaccgaaca gttgctgtaa
     9961 caactctttc atagggtttt gctccgtttc cgattttaaa atttgcgccg acgctgccgc
    10021 cagcacctcg tccataccgt ccaacgcggg gaaatttgtc agcgcggcgt gaaaaatttt
    10081 gcttacatat ccctttgtgt cataggaaaa cacagcagag atgtagcgat actcttttgc
    10141 ggcaattgcc gcagccgcct tgtccgtcca ctccacttcg gcaaacatgc ctttaggcgt
    10201 gaactccagc caacgcatcc aaccggcggc aggtgcaggt tgtccgtttt tctctttgta
    10261 gagcgtctgg tgttcataat cgacaaccaa ctgattgcgc gagctgttgg ccaacaacgc
    10321 gacatcatga ccgttttctt cggtcagata ccacgcaggg acatcagtcg gacgaccgtc
    10381 tactgcgcga aattcgccat atggcagcag ttggattcgc ccgtcttttg gctgcacctc
    10441 gaaactgcac acggcaagta gggttttttg tgcatttttg gacatgtgta cctctctcaa
    10501 tacggtcatt ttgcctattt gaaaaaacaa caaagagtag gcgtgcttca aaccctcccg
    10561 ccattttttt aaaacctgtc cgcgcgatac acaattaatc gttttcaggg gctttttaga
    10621 agcgtctagg attgattttt atatcttggg caggggtttg tattacccgc ataaaaaaac
    10681 gcgctaaaaa gcgcgttttt gagggtttta taaattcacg gtatcagacc cgaaaaataa
    10741 tcctgcacat cgtccatcaa agcctgtttg tcgtcgtccg tcagcgtcaa aaattcccgt
    10801 tgcggaatcc gaactttgcg gttgcgcccc gccataccgc cgaagttgtg gatggcggca
    10861 tagacgatat tcgtaccgac aagggcggta tcgttgtctg acagtgtgga aaaactgtct
    10921 ttcagacgac ccgaatccga aagcggcttg ccgtcgcggt attttagccc caaccatttc
    10981 ggacggcctg cgtagcggaa attgagcttg accgccgtgt gcatggtttc ggacagtcgg
    11041 cgcatcagca ggtagcggtt ttcgatcccg ttgccaagcc gctcgatttg gtttaggacg
    11101 acaaagatat tgtctatttt gacatcaatc atggtattat cctgcctagg ctactaaaaa
    11161 agcggtgaat ttgtctgccc tccgcagccc ttatgcagtc ctcggactgg gattggtatc
    11221 cgttacataa gcgagagtgt gtcgggattg cagtccgact tagtagcctt ttttcatttc
    11281 atagcgtttc ttacttttca caatttcttt ttcatccgcc ctgcgaaacg aatccaaaaa
    11341 aatcgaatcg tactgtggca gatatttcaa aactgccatc aatctgtatt tttcgttccc
    11401 ctccaacggt tttccgagca aaacaaaact ttcttttcct ctgcttttgt cataaaaaat
    11461 atattcgggc tcatgaatca gatcggggag catggcatat aaatctgttc caaacgattg
    11521 cccctcccga ctgtccacct gtttgaccaa tgtatcatca gacagccaca ccgtcgctcg
    11581 tgtcatacct gccaattctt gcgtttcctt gctcaataca cccgcagcaa atttaagctg
    11641 tcttgatagc gcatttcgga ttttgatttt ctgctcttta tcgggcttgc catcaatatc
    11701 caaacgttgc ttgacttcat aaaactcttt ttcaagctgt ttaaagctgg ttttaaaatc
    11761 cgcaccaccc atttccgctt tggcaaattg atgcgccaac gcacggtcgt acttgtctaa
    11821 atcggggcgg tagttcattc gtccggcgtt gtaatcaaat cctcgatcgg tcgtgtacag
    11881 actgccatcc ggtgctttat aggcaagggt cagataagta tcgccttttt tgttgtagat
    11941 tttatgggtc tcgactagat tgtccgccgt gctttgcccg acaatccgcc cctggcgttc
    12001 cacatcccgc tccgacagcg cgatgaccga gcaacggcag ttgtagccgt tgggcgggta
    12061 aaacgttgcc caaaacgggt cgtcgtagcg gtacaccagc ccgtctattg ccgaatgcgc
    12121 cggacgggtg cggctgtcgc ctaccgcgtc atacatccaa tagggccgtg catcaatatt
    12181 tgccatatat ccttgatatt gaccggcgtt gtaggcagtt tgcatgttgg tacggtaaat
    12241 cgtctccaac ctccgcggcg aaccgaatac ctcgccggtg gctgggtcga tgatatcctt
    12301 accgttatgc ccgttcggat gcagccagcc cttgttgccg agcagattca agataccttt
    12361 tcgccaatcg tcaaacgact gtccactttt tgccgattca accatcgaag ttttgatgtc
    12421 gttgagcata tccaagtcgg tcatgcgggc aatcgtatag actttggcaa tttcggaggc
    12481 tgtcagattg cggtagctct ccgccgtaac ctttttactt tccagccact cgattgcctt
    12541 ttttggcggc agacttaagg caaatcccaa atcaggcgcg ggcatggtct tgtcccaaaa
    12601 tatcgctgat aaacaaggcc tgctgcatat aggtacgcag tttcgcgttg tccaaattcg
    12661 gataaagcgc attcagtgcg gcatctgcct cctcgtagct gttgcaagca ttaagtgcgg
    12721 caaccgcctg acgcaccatc gggttgagct gagaattgaa atcgggctca accagcgcgt
    12781 catccaacgc gccgtccaat atttcctgat gcctgcccgt agccttagat ggtacggtgt
    12841 gggcggataa agccgccaat gcagttctgt ttatcggatt gtccggtacc tgccgcacca
    12901 acacagcctc accctcctgc acatctggaa tgaccagttt gtcgcgcacc cagctttcgg
    12961 ggatttgtac gccgacatcc accagtttcg ggatagcgtc ggcaaagacc gcgatgtctt
    13021 tcggctcgcg cgtgtcaaat tcaaatttcg gcacgcggtt tgggtcggca ttgggatagt
    13081 tgatttgcag gaacggtccg atgatttggc ttgtgatggt ttgcgccacc tgttttgcgt
    13141 ccgacaccag caaatcgcgg cgtacctcgt tgtggatatt gcccagcgcg ttggtgctgg
    13201 attttccgtc cgcaccgctg gttagcgttt gccccaaaat cagccgcgcc gccgattttt
    13261 cgcaccagtc ggccatctgc aaaaacggat tgctggttgc catcgtaccg cttgcagcgt
    13321 tgtgcagctc gatttccata ccttctggca tgatgcctgc cgcgttgtga ccgatttccg
    13381 ccaccgctcg aagcagggtg tttttttcct ctttggttgc gcccgcgccg tatttgccga
    13441 tacggatggg catgccgtac agctccaaaa actcggcaaa atcgtggacg gcgtagtgtt
    13501 tgaacatata cagccaggaa agcgtgcgga aaagcccgtt gcgcgcctgc tggacgctgc
    13561 gcgatttttg ggtatgaacg acccagccca gcggccacaa cgcttcgcct tccggatttt
    13621 cgcgggtacg cagcagcagc ccgttgtctt tgtcccattt gaaccagctt tgcgggcggt
    13681 ggataaagtt tcggggtagg taaaggccgt ctgaaaatac ccactcgacc tccaacgcag
    13741 aaaatccgtg ccctaccgcg tccatcaaat ccataatcag gtcttcgagg gtaggcaggc
    13801 tgtccatcat ttcgtaggct tggtcggaca gcttttcttc ttcgggcgtc gcatttcgcg
    13861 gcggggcgac gcgccagttg agcgtcagca gcgcgcgttt gcgcgtcccc atatttgccg
    13921 cgatgtcgct gtcgcgctcc tcaatgtccg cgaaaagctc gtgttgggcg cggatgtcgc
    13981 cgctttccgc atcctcgaag agggcccgca tcttttgcgg tgtaataaaa ttggacggat
    14041 gctcggcgat aacccgaccg gtcgccgtaa tttgagccac gtccgtctgc aatgcagctt
    14101 cgggcttttg gattttagtt ttattgttct tttttgccat tatcggctcc atttactttt
    14161 tatctcgtca tcaaaatcgt tatcggtagg gctttgccat tctatcggca cacaattggc
    14221 caccgccccc gaccagagca tatgcactgc atcaggacca tcatcatgat cagcctttgg
    14281 aaaatggcgg aactgctgta tcagcgtctg ttggctctca ttcaacagaa tcaaaccgtt
    14341 cgccatgtga ggctgtaaag tctcaatccg caagagcttg tccgataccg gcttgaccgc
    14401 ccgcgccggg acaggtattc cacgcgccgc gctgcgcttg accagctcat ctttcaaaaa
    14461 ttcctgaaac tgaaccgtct caacaaacca cagtttgcaa cgatattgac ggtgcaatct
    14521 gataacgtcc tcaataatca aatcagggag acgtttttta atctgagctt ccacgacata
    14581 cagtttgccg gtttcacgtt gataaccgcc aatgataatc gccgacgggt cacggctcgc
    14641 ccccgcttta cccagcgacg ggtcgagcgc accgaaatac accaaatcgg acggcaggtc
    14701 gttccaaaac ttcatcgact tggcaaacgg cgcatcttcg ccgctgaccg ggtcgttttg
    14761 atactcgctg tcaaatgtcg catgaccgtc acgggcgcgg attttcatca gcgcgagtac
    14821 gccgcgagcc gcccagcttg tttgcgcgcc gcgctccatc tcgtctttat tggcgagata
    14881 aaacgcctcg gccaccgtct cgccgtcgtt tcggaaaagt tcctcccatc tgtcccacaa
    14941 gtccatgcgg tcgggccagc gtttcatcgc cttaaactta acaccccgcc aaaacggatt
    15001 attcagggtg cggtttagca cgctgtcgta atgcaaaatc gtgccgatat aaatcacatc
    15061 gtatttctga ccgacaccgc ccaaaggcaa tacagtctta gtcagccacg cattgagctt
    15121 gtcgcgctgt tcggggttgc ggacttgctc gtcattctca atatcgtcca atatggtcag
    15181 gtcaggacgg taagggccgt gacgcaaacc gcgcagcttt ttaccgctac cggccacttg
    15241 gactttaacg tcattggccg tcgcaatcgt accggcctgc catacgcggc cctgcccgca
    15301 tacttccgga aagtcggttt tcaagcgcgg attaaattcc aattccgcct tgatggcttc
    15361 gagcatcggg tatgcctgat ctatgctgtc catcacaata acggcataat gtttttggcc
    15421 tgtcacaata caccacagcg taaacaactg agtaacctgc gtcgacttac cctcgccacg
    15481 cggcgcaccc accgcctcat tttccccttt gggggagcgg ataatctccg gcagacggct
    15541 gaataaaaac gcatgcagtt cggatttttc aggggagcgg atataatgcg gaaagtaggt
    15601 gttgacgaaa tactcgtaac cgcctaccgg gtcaaacacc ttggcacggc gtgcagcaat
    15661 agccttgggc aacgcatcaa agccgtccac ttccgcttcg ataacttggc gcaaactggc
    15721 ggccagttcg gcaagggatt tgaggaattc ttttgttttc atgagtattt ataatgactg
    15781 gattcatttc taacgcattt ataggcctaa aagaagcagg atcactttta aaagggttta
    15841 accagctcaa aacagaggca gaggtagcgc aaaaaaccat cgaacttaat caaatcattg
    15901 ctggtattca aactgattta tttgaagccc aatcttctta tacggcggca attagccgta
    15961 tagatgagtt ggaaaaagaa ctggtggcag taaaaaattg gtctacagag aagcaacgct
    16021 accaattgta tgaacttgct cctggttctt tcgtctatcg attgaaaaca gagatggcga
    16081 acagcgagcc aattcatgat atttgtcccc aatgttacga gcagggtgtc aaatcgattc
    16141 ttcaattcaa tgggtatgaa ggtgcttttc ataaatattc ctgttcccgt tgccaaacca
    16201 taatattggg agagcgttta aagtcagatg ttgaagtatt ttcagtagga ggatcacgtt
    16261 atgggagatt gatagacggc tattaaccaa acttcttctc cacttccgcc ccaaacggct
    16321 ccaatacctc cacaaaggca ggcaaatgtt tgggatgttt ttcttgcaca aacgccatca
    16381 agaactcaat caattccaaa gccgtcgcca gttttgacgt ttccggcatc acacgcgcat
    16441 tggccgatac ggttttcgta aacgcatcgg ccaggctggc caacagcttg gcgcggtcgg
    16501 acgggggcaa atcttcggta ctcgaatcct gcagcatcgt catcgtgctg ttgtactgca
    16561 ccataaaacc ggccaacatc gcacggctca agtcctcaat accgccgccg gccaaagtgt
    16621 aggcggcgcg catcttatcc cagtcgtcgc ctttttcctt atccgcacgt ttccacgcac
    16681 gcgcagtggt ctgcgggatt tcgcacatca aggccgccgt ttccaaagtt tgctcgccgc
    16741 tcacatagag ccgacgtaac ttttcacgga tttcttgcgg gtgagccata attacagtcc
    16801 cattttcgct ttaagcaatt cccagccgac cgtaatcacg ccgccgccca gtgcgccgaa
    16861 tgtaatggcc gtgcgtttcg tgtcttggcg gatttgcgca atttccgcct gcatttcttt
    16921 ctgatttttc agcgtttgat cagtcttgtt ttcaatacgc gccaaggctt ccaaaatcgg
    16981 gtcgctcatg atttgtctgc tttcctgtcc agtttttcgt ttactttttc caacttgttt
    17041 tcgatgcgtc ccaacgaagc cgcaatattg tcgcggtctg ccttggcatc ttgcttggtg
    17101 tgataagaga gcttgaccgc gtgcagctcc tctttcaggt cgtcgatacg cttgtccgcc
    17161 tctttcagac ggcctgaaat gccgttgacc caaaaccaaa atgccgccgt cgcaatcggc
    17221 cacagggttc tgaaaccaaa ttcaaagtcc atttaaaacc cctttaaacc ggcacatcgc
    17281 cgaatacgat acggacggcg taaccgtcag ggcgattgct ggcaatctcc agtccatcac
    17341 catcattaca aacacagtaa tacgccccca aagtttgcca aaccgcacgt ttaaaggtgt
    17401 catagttcgt aattggatat tcaaggtgaa acgtcgtctg aaaatcctta tccatgcgta
    17461 ccgcgtactc aatgcctgcc ttatccaaca gatcggaaac atgaatgaca aacggctctt
    17521 gctcgcgtgc gcggcttaag cccaattcca aatcggcatg gcgcacagcc aactgacgtt
    17581 caactaattc acggtaggtc attctttgat acccattaaa tattttgtcc gcttgtacaa
    17641 tttcttaacc cacgaaatat ttacaaatgt ataaaccttt gctacaactt cgccgtcata
    17701 gtgcgcattt tctcgtgcag cccgaaattt cgccctggct tcttcagggt tgtccgccca
    17761 aatgctcaat gaccaggact tgccgttaaa gcggtaagaa aacgtgtact cattcatagg
    17821 agaaacctta tgtattttga aatctataaa gacgcaaaag gcgaataccg ttggcgtttg
    17881 aaagcagcca accatgaaat catcgctcag ggcgaaggct acaccagcaa gcaaaactgt
    17941 cagcacgcag tcgatttgct gaaaagcact accgccgcta cccctgtaaa agaggtataa
    18001 aatccgcttt caccctcagc ccgcgcccta cgcgggcttt tttgtcagtt tccgactttg
    18061 cgggagtgat tgcccgccca gtcgcgccaa gcctgatttt gattttccaa ctccgccaca
    18121 tagccgccaa actcggcggc gtgttccaac agcgtggccg tcttgccgtc tttcggcgga
    18181 tttgggcgta ccggcgcgac catcaatgcg gcaggcggtg tcggcatgac cgccttttcg
    18241 acaaccttaa tttccgtagc cgagggcgcg cttgtagagc tgcaagccgt gatggccaaa
    18301 gccgtcaata caaccgccgc ctgcattttt acggtcttga gtaaggacat tttcgatttc
    18361 ctttttattt tccgttttca gacggctgac ttccgcctgt tttttcgcca aagccatgcc
    18421 gacggcgtgc gccttgactt catatttttt cgcttccgcg cgcgcctgtt ccagctcgcg
    18481 tgcatagttt tgagccgaca acagcatggc ttgcgccttg tcgcgcttca ttttctcaat
    18541 gaccacctgc tgcttcgcaa acgccgactt atagccttga tggtgcgaca ccgccaaacc
    18601 cgtgccgaca agcgcgataa tggcaattgg ttgccagtta ttcgctagca gtttcacgag
    18661 attcattctc gacctcctga cgtttgacgc tgaccaacga acgcgccacc gcatagccgc
    18721 cgacaatgcc caaatacacc gcccaaatct ccgccgatgg gtcgggcaac atcacaaatt
    18781 taaacgtccc cgccgcgcag gcgacgtttg cccacagctt cgagtgcgac acattgcctg
    18841 tcgccgggtt tttaaaaata tccaaaatac gcattgctat tccacacttt tggtttgcag
    18901 gtgccgtttc agcatttcct gataattggc cagttcgccc tccgcaaatt caaacgccgc
    18961 caagtctgcc tgttcgcttg cctcacggct tttggccgac cacagcccaa tcatcttttc
    19021 gtaaaacgca acctgtccca tgattaacga cgattcttgc gtttgcgcgc agcccgtttc
    19081 gcggctgcca cgcctgagtt tcttaaattt gggcgtggat aggtgtgtat cggacgaaca
    19141 tttggcatct gtataagtgc gcggtttacc tgctcattac taggattttc tccaatcaat
    19201 tttgtaagaa aataggaagc agcttctttc aatttaccaa taatattcat actcttgccg
    19261 cccccaattt catcgcaatc gcgtccgcaa tcgcgcggca gatgccccat ttggtcgtct
    19321 taaacaaggc caaatcagtg tcgttgctga tgaaaaaagg ctcaacaatg ccgccagcct
    19381 gcgcataagc caggcgcgaa tgatggcccg cattgtcggg tttaaagccg tcttcgccgc
    19441 gcagtttcca gccggttttc ttggcaacag ctttgctcaa cacctgacac cagcgtttgt
    19501 ttttaacggt actcaacgcc tcaatgcccg tagctgcttt accggccgcc gagttggtat
    19561 ggaactcaat cgccacatcc gagccgcgaa tcagttttac agcttcacgc agcggcatat
    19621 tgcctttgcc tgtgccgtcg gttttaacag tcaaaccgta gtcatcgcgc aaaatagcag
    19681 ccacaatgtt gcgcatatcc tgcgccaagt ccgcctcacg gtcgctgccg ttgaccgcac
    19741 ccgggtcggt gttgctgtgt ccagcggtta aggttacggt tttgcccatt catcatctcc
    19801 aaaaaatatt gattgcatat agtggattaa caaaaatcag gacaaggcga cgaagccgca
    19861 gacagtacag atagtacgga accgattcac ttggtgcttc agcaccttag agaatcgttc
    19921 tctttgagct aaggcgaggc aacgccgtac tggtttttgt taatccacta taacatttga
    19981 aaaccccatt aaaccgtctt taacccatcg tcccgaacgg tagatgtttc acccctgcaa
    20041 accaaaaaaa agaccgtctg aatacagacg gccaaagcct ggtcacacat cactgcctaa
    20101 aataaagctg cctgctgtgc cgcaggtcgg ctcatttcat tgataatcgt atatcccgtt
    20161 cgtgaagaga tgccgtattt agggcatagc ttcgtcatcg ccataagccc gctctcctta
    20221 tcaatatcgc gcaatttgac aaactcctga taaaacctat ggtttctcaa ctgtattaac
    20281 gccttgccgc accgtgggac atacaattcc tcgccaccat atacctgcaa cagctcatgt
    20341 gttttcactt cgccgatggc ttcgaccaaa attgccaaac gctcggtgtc caccttgccc
    20401 ttaccaaatt taaaccgcgc cccgccaatc gccttgacca gctgttccgt cgctgccagt
    20461 ccgatgacat ccacaatgtc caacacggta tccggcaata aatgttcaac tttttcgaac
    20521 cccatcatcc ccacccgctt tttccgtttt cctgttttcc gcaatctgca acgcagcaac
    20581 cagtttatgt agctgcgtat cgtctaaata ttcgacctta tccttaccaa acatccgccg
    20641 cgccattgcg tgtgcatagt tccaatgttt gccgccgacg gtcagcaggg cttcgacttt
    20701 gtccaacatt gccgctgatg atgtccgacg cagatgtggt ttaccgtgtg ggttaccttt
    20761 tgctttaggc ttaaatccgt gcgaccgcat atcagcgaca acagactcaa gttcggaaac
    20821 atccatatcc gcacacgacc gcttgcccgt cacgcgctcc aacaccgcgc gataggtacc
    20881 gtcgtccaag cccagctcct tttgggcaat tttaattttt gcaatcaacg cccggcgcat
    20941 ctcaaaccca taaaacacaa tatattgact aattaacgca tattatacca ataaaataca
    21001 atatatagta ttaagccgat gttttttgcg aaacggacag acatgaaaaa gcaactgtat
    21061 ttttcacccc gtcgggcaaa aataccaaaa ctcaaatcaa gccgtccgga taccgttttc
    21121 ggcggtatcg ttttcggcaa aataatcacg catccgggca ttcgatatcg tcagcagttt
    21181 gcgcatacat gccgtaacgg caaccttata cggcttaccc tcggacagcg ggcgttggtg
    21241 gaaatcccga ataagcggtt caaaacgtgt cgctgccacg gtagccatat acagtgcttt
    21301 acgcaccgca gaccttccgc caaagcagcg gcttttgaat ttggtttccc cgccctccct
    21361 cgggtgcggg gcaatgccgg ccaaacccgc tatccgtttg tgcgacagcc gccccaattc
    21421 gggcaacatc gccatcagcg tagccgtcgt tatcgaaccg atgcctttga tttgctccgc
    21481 cacttggtct ttgccgtcaa aatgcgtgtg ggtgtggttg tcgatttgtt tgtccaattc
    21541 gtcaatcagc cggtcaaaat gggcaatcag ttgtttgacg cttccgactt gcgtttcgtg
    21601 aacctgatgc agacggtttt tctcggcagt ccgcatatcc gccggttggt tgcggcggtt
    21661 aaccaaggct tccaacactt cttccgcttc ggtgggcggt tggtagggca tggtttgcga
    21721 atcttctttc tgtgccgtca tctgtg
//
LOCUS       DAWHXZ010000007        20530 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000007 DAWHXZ010000000
VERSION     DAWHXZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20530
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.007"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(45..1226)
                     /gene="odhB"
                     /locus_tag="WIE35_000190"
     CDS             complement(45..1226)
                     /gene="odhB"
                     /locus_tag="WIE35_000190"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ4153176.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            complement(1326..4154)
                     /locus_tag="WIE35_000191"
     CDS             complement(1326..4154)
                     /locus_tag="WIE35_000191"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ4153177.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDTVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVVGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(4359..5642)
                     /gene="gltA"
                     /locus_tag="WIE35_000192"
     CDS             complement(4359..5642)
                     /gene="gltA"
                     /locus_tag="WIE35_000192"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ4153178.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(5754..6002)
                     /locus_tag="WIE35_000193"
     CDS             complement(5754..6002)
                     /locus_tag="WIE35_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ4153179.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6006..6713)
                     /locus_tag="WIE35_000194"
     CDS             complement(6006..6713)
                     /locus_tag="WIE35_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ4153180.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITSERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6834..8597)
                     /gene="sdhA"
                     /locus_tag="WIE35_000195"
     CDS             complement(6834..8597)
                     /gene="sdhA"
                     /locus_tag="WIE35_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ4153181.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8600..8941)
                     /gene="sdhD"
                     /locus_tag="WIE35_000196"
     CDS             complement(8600..8941)
                     /gene="sdhD"
                     /locus_tag="WIE35_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ4153182.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(8935..9312)
                     /gene="sdhC"
                     /locus_tag="WIE35_000197"
     CDS             complement(8935..9312)
                     /gene="sdhC"
                     /locus_tag="WIE35_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ4153183.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9591..10994)
                     /locus_tag="WIE35_000198"
     CDS             complement(9591..10994)
                     /locus_tag="WIE35_000198"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ4153184.1"
                     /translation="MKKIQADVVVLGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDSFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAS
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11243..11980)
                     /locus_tag="WIE35_000199"
     CDS             complement(11243..11980)
                     /locus_tag="WIE35_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ4153185.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12259..14535)
                     /gene="metE"
                     /locus_tag="WIE35_000200"
     CDS             complement(12259..14535)
                     /gene="metE"
                     /locus_tag="WIE35_000200"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ4153186.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQVAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTGFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLSKVNAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKNQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARSAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(14673..15551)
                     /gene="metF"
                     /locus_tag="WIE35_000201"
     CDS             complement(14673..15551)
                     /gene="metF"
                     /locus_tag="WIE35_000201"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ4153187.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15751..16026
                     /locus_tag="WIE35_000202"
     CDS             15751..16026
                     /locus_tag="WIE35_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ4153188.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16026..16151
                     /gene="ykgO"
                     /locus_tag="WIE35_000203"
     CDS             16026..16151
                     /gene="ykgO"
                     /locus_tag="WIE35_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ4153189.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(16363..16563)
                     /locus_tag="WIE35_000204"
     CDS             complement(16363..16563)
                     /locus_tag="WIE35_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153190.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALREVAEISAWVLLG
                     AAAAMLFWYLFMLYFKHIPDSY"
     gene            16713..17852
                     /locus_tag="WIE35_000205"
     CDS             16713..17852
                     /locus_tag="WIE35_000205"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ4153191.1"
                     /translation="MSQNASVGIVMPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPLVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            17849..18430
                     /gene="metW"
                     /locus_tag="WIE35_000206"
     CDS             17849..18430
                     /gene="metW"
                     /locus_tag="WIE35_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ4153192.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTSNRQVKHFPNLLGSLAFYRVG"
     gene            18547..19383
                     /locus_tag="WIE35_000207"
     CDS             18547..19383
                     /locus_tag="WIE35_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ4153193.1"
                     /translation="MKNKTSSLLLWLTAIMLTACSPNKNDKTKEVGASAASSSASSAS
                     SQSDLQPAASAPDNVKQAESAPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALSHHE
                     LETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDALME
                     QERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYIGQL
                     PGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNPNLG
                     RR"
     gene            complement(19433..19993)
                     /gene="efp"
                     /locus_tag="WIE35_000208"
     CDS             complement(19433..19993)
                     /gene="efp"
                     /locus_tag="WIE35_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ4153194.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(20036..>20530)
                     /gene="earP"
                     /locus_tag="WIE35_000209"
     CDS             complement(20036..>20530)
                     /gene="earP"
                     /locus_tag="WIE35_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ4153195.1"
                     /translation="LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLA
                     DCAVIRGEDSFVRAQLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETASAHRR
                     LSDDLNGGEALSATQRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKH
                     QKIR"
BASE COUNT         5048 a         5197 c         5178 g         5107 t
ORIGIN      
        1 tcagacggcc ttttcattaa caaataaaag gccgtctgaa acgattacag atccaacaac
       61 aggcgggctg ggtcttccaa cgcgtcttta atggctacca aggtcaatac agcttcgcgg
      121 ccgtcaatga tacggtggtc gtaagacaga gccagataca tcattggacg gacaacaact
      181 tggccgtttt caaccacagc gcgctcttta gtggcgtgca tacccaaaat cgcagattga
      241 ggcggattga tgatcggggt ggacatcata gaaccgaaag taccgccgtt ggtaatactg
      301 aatgtaccgc cggtcagatc ttcgatagcg attttgccgt ctttggcttt tttcgcgtaa
      361 tcaacaattg cttgttcgat gtcggcaatg ctcatttggt cggcatcgcg caggattggc
      421 acaaccaaac cgcgtgggct gccaatcgcg ataccgatgt cgaagtagcc gtggtacaca
      481 atgtctttgc cgtcaacaga agcattcaca accgggtatt ttttcagggc tgcaacagcg
      541 gctttaacga agaaggacat aaagcccagt ttcacgccgt gttctttctc gaatttttct
      601 ttgtacttcg cacgcaagtc catgattggt ttcatgttga cttcgttgaa tgtagtcaga
      661 atggcgtttt cttgttgaga agccaagagg cgttctgcaa cacgggcacg caggcggctc
      721 atcggtacgc gttcttcagg acgtgcgccg gcaggaagtg caacggcagg agcagcggct
      781 gcggcaggtt tggctgcggc attttgtacg tcttctttca atacgcgacc gtcacggccg
      841 gaaccttgca atgcgttcac gtcaacaccg ctctctgcag ccagtttggc ggcggcaggc
      901 atagcggcgt tgttttgtgc agcagcagga gcggcggccg gggcagcttc ggcaggagcg
      961 gcggcaggtg cttcagcagc ggcagtagca gcggtatcga tacgtgccaa aacttggtcg
     1021 gcaacaacgg tttcaccgtc ttgcgctacg atttcaacca atacgccggc ttgtggagaa
     1081 ggtacttcca aaaccacttt gtccgtttcg atatcgatca ggatttcgtc acgggcaact
     1141 gcttcgccaa cttttttctt ccattccaag agcgtgcctt cagatacgct ttcagacagc
     1201 ataggtactt ttacatcaat aatcattttg tgtctccaat ggccctttca gacggcctgt
     1261 tgtgtgtttt tcattctgca tatgggcatt ccgcctgagc agcctcagac ggaatgctgc
     1321 ttggtttaca acgccaaagc gtcttcaacc aattgtttca attgagcaat gtgtttgctt
     1381 gagtagccca ctgcaggcga tgcgctgctt ggacgaccgg cataagacag tttttgctct
     1441 tcgctgataa cgtcttcgat gcggtggcgg atttggtaga acgcgccttg gtttttcggc
     1501 tcttcttgcg cccaaaccac agattttgcg ttcggatatt tcgccagttc ggctttaacc
     1561 tcatcgtatg ggaacggata cagctgctca acgcggacaa tcgcaacatc atcttccagt
     1621 ttacgttcgg ctcgaccggc ttccaagtca tagtaaacct gaccggcaca caataccacg
     1681 cgtttcacgc tgtcgttgct tgcgcgttcg gcggtatcgc cgacaaccgg acggaaagtc
     1741 gaaccttcgg tgaagttttc cagcgggctc attgcccctt tgaatcgcaa caggcgtttg
     1801 gacatgaaaa ttaccagcgg tttgcggtat gaacctaaga cttgacgttg caagaggtgg
     1861 aacatttgcg acgcttcaga tggcatgatg acttgcatat tgttctcaga acacagttgc
     1921 aaccagcgtt ctacgcgtgc agaagagtgc tcagggcctt gaccgtcgta accgtgcggc
     1981 aggatggtgg tcagaccgca caagcgaccc catttggttt cgcctgaaga caggaattgg
     2041 tcaatagtca cttgcgcgcc gttggcgaag tcaccgaatt gagcttccca aatggtgagc
     2101 ttgtcaggag cggagcaggc aaagccgtac tcgaacgcca tcacggcctc ttcgttcaag
     2161 atggagtcga taaccaggaa ctcgcccatt ccttcgccca tatggcgcag gggaacataa
     2221 gtaccgtcgt cccatttttc gcgtttttga tcgtgcaata cggcgtggcg gtgcgagaac
     2281 gtaccgcggc cggagtcctc accggagata cgcacgccat gacctttggt tacgaggctg
     2341 gcgtatgcca aggtttcggc cataccccaa tctatggcct gtttgccgga tgccatggct
     2401 ttacgcgctt caatcacacg ttttgcagtc ggatgcaggg caaagccttc cggtacggcg
     2461 gtaaacttct cagtgagacg ctcaatatct gcggcaggca aaccggtttc gatgtgttcg
     2521 cgccaatctt tgccttggta tttgctccag tcgatttgcg tgcgttgaaa gttgctcaac
     2581 gttgtttgct cgacgtgttc gcctttgtcc aaagcatcac ggtaggcttg gatgtaaccg
     2641 tctgcctcgg cttgggttac cacgccttcg gcaatcagtt gctcggtgta caaagcacgc
     2701 gcaccggggt gttgcgatac ttttttgtac atcatcggtt gggtcaaggt cggatcatcg
     2761 ccctcgttgt gaccccattt acggtagcag acaacgtcaa tcacgatgtc tttatggaat
     2821 tttttgcggt aatccaaagc ggcttggatg gcaaagcaaa cacgctcagg atcatcgccg
     2881 ttcacatgga taaccggagc ggaaaccatt tttgcgatat cggtacagtg tacggttgaa
     2941 cgggtatcgc ggatatcgga agtggtaaag ccgatttgat tgttgatgac gatatgaacc
     3001 gtaccgccgg tggtataacc gcgcgtttta gacaggttga atgtcgcttg gttgacaccc
     3061 aaaccgataa atgcggagtc gccgtgaatc aatacgggca agactttatc gcggccgttt
     3121 tcgcccaaac gtttttgctt ggcgcgtgca gaaccttcca ctaccggatt gacgatttcc
     3181 aagtgggacg ggttgaacgc caaagaaaca tgcatcgggc cgtgcggggt agcaatatcg
     3241 gagctgaagc ccatatggta tttcacgtcg ccgctgggca gtttgatttc ggcacgacct
     3301 tcaaattcgg caaacaaatc gccgggtttt ttgcccaaaa tgttcaccaa aacattcaga
     3361 cggccacggt gcgccatacc gatgatgact tcttccacgc cgtctttacc ggcgttttga
     3421 atcaggtagt tcaaaccggc aatcgcgctt tcgccgcctt cgacaccgaa acgtttctga
     3481 ccgacatatt tggtatgcag ataacgttcc aaagtctcgg cagcagtcat ctctttcaag
     3541 atacggcgtt tttgatcggc attgtaatgc ggtgtggaca atacgctttc aaaataattg
     3601 cgtacccagc ggcgctcttc ggtattggga atatagatat attccaatgc gatgtggccg
     3661 cagtaggttt gtttgaggtt gctgatgatt tgggacaaag gcagtttgcc gcgattggca
     3721 aaatcgccct cgcccatatt gaattgaagc gccatatcgg catcagacag accgtggaat
     3781 ttcggatcga gggcttcaat atcgcgcggg gggatacgtt tgagcggatc aagttgggct
     3841 gcacccacgc cttggatacg ataggcagaa atcagccgta aaacgctgac ttgctttttc
     3901 agcattgcct catccgcacc gcccgcaacg gcagatgcaa ttttcttttt cgccaaagta
     3961 acaaatgatt cgcgaatcgg tgtgtgtgcg acatcgacag caaccgtccc cggctgtttg
     4021 ctcaaatcgg tgaaatactg cttccatttt tcatcaaccg tatcggggtt ttccaaaaaa
     4081 gcctcgtaca attcctcaat gtagggtgcg ttcgaaccga acaggtaaga gaaattgagt
     4141 ttttcgtcca tcatggcgtg cgctcttttc tttaaaatat aaacaaaggg tagaagtccg
     4201 cgtcctgacg gctaaactga tgccgtctga aaagacattt tattctaccc tattttaaac
     4261 cggattgctt gtcttgattt catccgacta caatcagacg gattcgggaa acaatccggt
     4321 tctctgatat tgcctgtttg acaatatatt aatgtatgtt acctctcgcc tggcggcaca
     4381 taatcgcggc gttccgaacc ggtataaagc tggcgcgggc ggccgatttt cagcgaagga
     4441 tcgctaatca tctcgtgcca gtgcgaaatc cagcccacgc tgcgcgacag ggcgaagatg
     4501 acggtaaaca tttcggtcgg gatgcccagc gcggacagga cgatgccgga atagaaatcg
     4561 acgtttggat acagtttgcg ttcgataaag aacgggtctt tcagcgcaat ctgttccaat
     4621 tccatcgcca gtttgaattt cggactgtct tccaagccca attccttcaa aacttcatag
     4681 caggtttcgc gcataatgct ggcacgcgga tccatattgc ggtacacgcg gtgaccgaag
     4741 cccatcagac ggtatttgcg ttgtttcaca ccttccatgt atgcggcaac attagacaca
     4801 tcgccgattt cgtccaacat tttcaacacg gcttcgttcg caccgccgtg cgaagcaccc
     4861 cacaggcagg cgataccggc agcaatacag gcaaacggat tcgcacccga agagcctgcc
     4921 agacggacgg ttgaagttga ggcgttttgc tcgtggtcgg catgcaaaat aaagatgcgg
     4981 tcgagcgcgc gtgccaaaac gggattgggt ttgtagtctt cacacggcgt ggcgaacatc
     5041 atatgaagga agttttcgga ataagaaaga ttattcttcg gataattgaa cggcagaccg
     5101 tttgaatagc ggtagcacat tgccgcaatg gtcgggattt tagaaatcag gcggtaaatc
     5161 gcgattttgc ggtgttcggg attgctaatg tccaagctgt cttggtagaa cgcagacagt
     5221 gcgccgacca cgccgaccat catcgccatc ggatgcgcgt cgcggcggaa gccccggaag
     5281 aaccaagtca gctgttcatg caccatcgtg tggcggcgga cggtattgtc aaattctgcc
     5341 ttttgctcgg gagtcggcag ttcgccgtaa atcaacaggt agcagacttc caaataatcg
     5401 gacttttcgg ccagctgctc gatggggtat ccgcgataat aaagcaagcc ttgatcgccg
     5461 tcgatgtaag taattttaga ctcacagctt gcggttgaaa caaatccggg gtcgaaggaa
     5521 aacaaacctg tattttttgt cagcccccga atgtcaacca catcgtgccc gatgctggct
     5581 tccaataccg gcagctccaa acctgcctga cccggtacgt tgagtttgat tgatttggac
     5641 atattattgc tcctttgcaa gttataaccc acgttactgc atccgcagca cgttctttgc
     5701 cttcagacgg cttttgcatt ttgaaatctg cctgcaggca gatttcagat gcctcatgcc
     5761 cgtctgattt tttcaagcat cgggataagg tgccctttgt ccgtttccga atgcccgtta
     5821 atcaaggcaa gcaattcttg atcttgaaat tcaaggattt cggaaaactc ggacagctct
     5881 ttatcgctca aatgctcgaa ttctttttcc ataaacctgc cgaagattaa atctaactcc
     5941 aacaatcccc ggcgggtttg aaaacggatt ttccgtttgg caatatcgtc aaaaaccatc
     6001 atttcttaaa cggcccgttt caacataatc tctttaatct taccgatggc tcgggtcgga
     6061 ttcaagtgtt tgggacatac gtctacgcag ttcataatgg tgtggcaacg gaacaaacgg
     6121 tatgggtcgt tcagattatc caaacgctca ctagtgatgg tatctcggct gtccgcaatg
     6181 aagcggtaag cattcagcaa accggacgga ccaacgaatt tatcagggtt ccaccaaaat
     6241 gacgggcagg cagtcgaaca gcaggcgcac aaaatacact cgtacaaacc gtccaactct
     6301 ttacgctctt cctgagtttg cagacgctct ttgtccgcat caatcggatt atcgttgaca
     6361 acataaggtt tgacggaatg gtattgtttg aagaactggg tcatatccac aatcaggtca
     6421 cggataacag gcagacctgg cagaggacga attttaaccg gctgtttcaa gccacgcaga
     6481 tcggtcaaac acgccaagcc gtttttgccg ttgatgttca taccgtcaga accgcaaatg
     6541 ccttcgcggc aggagcggcg gaaagacaag gtatcgtctt gtgctttcag gcgtaccaaa
     6601 gcatccaaaa gtttcacgtc ggtcggttcc aattccaact cgtaacgctg catataaggc
     6661 ttggcatcaa catccgggtt gtaacggtaa atttcaaaac tcattttttc catgagaagt
     6721 gttccttttc tcatcaactg cccaatgatt ttgtattata aattcaagca gttcaatgtg
     6781 ggtatggtta gatttcagac gagcttatga ggcgaaaacc gtctgaaaac gcatcaataa
     6841 acgcgcttgg ccggtttgat gtattccacg ctcaaaggct tggtatgtac cggtttgtaa
     6901 gacaaagtat tggtatctga atggtacagc gtatgtttca tccagttttc atcatcgcgc
     6961 tcaggatggt cgtctgaagc gtgcgcaccg cgtgattctt tacgtgcttc ggcagacacc
     7021 aaagtcgctt tcgccacttc aattaggtta tccaattcca aagcctcgat acgcgcggta
     7081 ttccacactt tgctcttgtc tttgatttcg gtacgtttca cacgctcggc aatcgccatg
     7141 acttctcgaa cgcctttgct cagaatctca tcagtacgga acacgccggc gtgcagttgt
     7201 acggagcgtt gcaattcgcg acgcaatgca tcaacgtttt cgccatcagt ttgattgtcc
     7261 aaacgctcga tacgttggcg ggtcagctcg cccgcatccg caggcaaagg tttccagtcg
     7321 ctttgctctt tgatgaattt aatcatgctg tcgccggcag ctttaccgaa taccaccaag
     7381 tccaacaggg agttggtacc caagcggttc gcaccgtgta cggaagcaca agcgcactca
     7441 cctgccgcat acagaccttt tacaggcact tcgtattcgt cgccttgagg aacgacaact
     7501 tcgccatggt agttggtcgg aataccgccc atcatatagt gggtagtcgg cacaacggga
     7561 atcgggtctt taatcggatc gataccggcg aactgaatgg aaatctcgcg gatgcccggc
     7621 agtttttcca taattttttc tgcgccgata tggtcgattt tcagtaagac atggtctttg
     7681 tttttaccgc agccgcgacc ttcgtagatt tccatcgcca tcgcgcggga aacaacgtcg
     7741 cgagaagcca agtcttttac ggtcggcgca tagcgttcca taaagcgttc gccgtcggca
     7801 ttcaacagaa taccgccctc gccgcgtacg ccttcggtaa tcaacacgcc cgcacctgcc
     7861 acgccggtcg ggtggaattg ccagaattcc atgtcttcca acgggatacc tgcacgcgcg
     7921 caaataccca aaccatcgcc ggtattcata taggcattgg tagaagaggc ataaatacgg
     7981 ccgccgccgc cggtagcaaa catcacagct ttagcgtgga aaatataaac ttcgccggtt
     8041 tccatttcca tggcggttac gccgacgaca tcgccgtttt catcacgaat caaatcttgt
     8101 gccgtccatt ccacaaagaa ttgcgtattg gcacggacgt tttgttggta caaagtatgc
     8161 agcatcgcat gacctgtacg gtcggcaacc gcacaggcgc gttctaccgc gcgtttaccg
     8221 tgttcggcag tatggccgcc gaaaggacgc tgataaattt taccgctttc cacacggtca
     8281 aaaggcatac ccatgtgttc caactcaatc accgcttcag gcgcagcgcg gcacataaac
     8341 tcaatcgcat cttggtcgcc cagccagtcg gaacctttca cggtatcgta catgtgccag
     8401 tcccaacggt cctcctgcac attacccaaa gaggcggaaa taccgccctg cgccgctacg
     8461 gtatgcgaac gggtcgggaa cactttagac aaaacggcac aattcagacc ggatttggat
     8521 aattggaggg ctgcgcgtaa acctgcacca ccaccgccga caatcacggc atcaaacttg
     8581 cgaacaggaa aacccatact tacccccaaa ttactttaat tgaatacacc aagcagccga
     8641 ccagccagac gatggtggca acctgcaaaa acaaacgcac gccgaagggt ttgatatagt
     8701 ccatccacaa atcgcggata cccacccaag cgtgcaagaa tacggcgatg aagctcactt
     8761 gggtaaatac ttttacccaa gtttgactaa aaaatgcctg ccatgccgaa tattctttag
     8821 gcagggaaaa tagaaccact aaaagtgcaa cggtataaat caacataata accgcagtcg
     8881 cacgttgcat cgcccaatcg cgcaaaccgt aatgggcacc ggtcaattta cgttctacca
     8941 taacaacgct cccaaaacga cagtcaaaac caatgcagaa gcaaatacgg ctttagcggt
     9001 attgcgcgca gtattcagct caaggccttt gtgcgcatcc aaaaataaaa agcggatacc
     9061 ggcgagagaa tggtgcagat aagcccacaa tacaccgatt aaaaccagct tgaccaaagg
     9121 atgggaaaca atggcacggt aagtttcaaa tgcagactct tgactcaggg taccggacag
     9181 gaaatacagc aggaaaggca gcataataaa cagcccgacc ccactgatgc ggtgaaggat
     9241 ggaaactatc ccgggtatcg gcagacggat gttcggcaaa tccagataaa caggacgcgg
     9301 tttggcagac atagtcaatt cctctgtagt tatatatcga ctcaaaaacc gtaaacccat
     9361 tgctacactg ggttacataa acgttaattt acctgtttta tcccattttg aacagtgtat
     9421 tttaaaaaca aactgataga ttttgacaaa cacgcgtgga aacagcagat taaaagaaag
     9481 atttttagtt ttgccgcccc cttcccgaca cggtatcagc atccgacccc atccccaaaa
     9541 acaaaatgcc gtctgaaaaa atttcagacg gcattgtttt gccatatcgg tcaggctttc
     9601 aatttcgcat cggcatcgcg caacgcggta cgcagacctt cctcgataac gggatggtag
     9661 aacggcatat ccagcatttg cggaacggtc atcttcattt gatgcgccca tgccaacaag
     9721 tgcgccaaat gttcggcggc agggcctacg atttccgcgc cgataaagcg gccggtggct
     9781 ttttcggcat acaggcgcat atggcctttg tttaccagca tcacgcggct gcggccttgg
     9841 tttttgaacg atacttcgcc gatgacaaat tcgtcggctt ggtattgcgc ggcaacctgc
     9901 gcgtatttca gaccgacaaa gccgatttgc ggactggtaa acaccacgcc gatggtgctg
     9961 cggcgcaaac cgccgccgat attcgggtag cggctcgcgt tatcgccggc aatcttgcct
    10021 tggtcggcag cttcatgcag cagaggcagt tggttggacg catcgcctgc gatgaagata
    10081 tgcggaatac tggtctgcat ggtcagcgga tcagcaacag gtacgccgcg cgcatctttt
    10141 tcgatattga tattttccaa accgatattg tcaacgttcg gacggcggcc cacggctgcc
    10201 agcatatatt cggcaacaaa tacgcctttt tcgccatcct gctcccaatg gacttctaca
    10261 ttgccgtctg catcgagctt gacctcggtt ttagcatcca aatgcagttt caattcttcg
    10321 ccgaacacgg ctttcgcctc gtctgaaacg acggggtcgg aaatgccgcc gatgattccg
    10381 cccaaaccga aaatttcaac tttcacgccc aaacggtgca atgcctgacc cagttccaaa
    10441 ccgataacac ccggcccgaa cacggcaacg cgcttaggca gcgtatccca tgagaaaacg
    10501 tcatcgttga taatcaaacg attgcccaaa gactgccatt gcggcaggat gacgggacgc
    10561 gaaccggtag caatcacgaa actttttgcc gtaatttgag tatgctcgtc gatttggacg
    10621 gtatgctcgt cgataaattt agccgaaccc atgatgcgct tgtcggcagg ccactcttcc
    10681 acatcggcaa cgacaaagcc gacaaaacgg tcacgctcgg atttaacgcg ctgcatgacc
    10741 tcttcaccgt tgacgacgat gctgtctttg tccaaatgca cgccgaacga gtcggtatgc
    10801 aatgcgtggt ggcgcgcttc tgcggcggca atcaagagtt tggaaggcat acaacccacg
    10861 cgcgcgcagg ttgtgccgaa cacattgttt tcaatcaggt aaacattatc cgaatgtaaa
    10921 cgggcattgc gaaacgcacc cataccggca gtaccgccgc cgagtacgac gacatccgct
    10981 tgaatttttt tcataatctt tgtccttccg ttttcgtctg aaacattgcc gacacagaac
    11041 atatcggttt cagacggcat ttaaatattt tcccaatcaa aaacatcatt cagataaata
    11101 aaaacaaaat ttcgattgag tgcaacaata tccaaattct cttataaata tagagttatt
    11161 cggagcagca gtgcagaacc gcccgaataa ctctatccgg tatatcagac agtttaatca
    11221 aaccgcctta agcaaacagc tatcagtttt tagccaagta agcttccaaa tcttcgctgc
    11281 cgccgatgta tttgccgccg atgaagactt gaggggcagt catcttgccg gtaatggcgc
    11341 gaacggaagt aacggttgca tctttgccca atacgatttc ttcgtaagac aaacctttgt
    11401 cttgcaaagc ttgtttggct ttagcgcaga attggcaacc tggtttagtg aaaattgcca
    11461 cagactcttg agccttccaa tcgggagcaa cgaattgcag catagtatct gcatcggata
    11521 ctttgaacgg atcgcccggt tcttcaggtt cgatgaacat tttttcaacc acgccgtcgt
    11581 taaccagcat ggagtaacgc caagagcgtt taccgaagcc caagtcttct ttaccgacca
    11641 gcatacccat accttcggta aattcgccgt tgccatcagg aatcatgtag atgttgtcgg
    11701 attcttcttc ggcagcccaa gcgttcatta cgaacgtatc gtttacagat acgcagtaga
    11761 ttgcgtcaac gccgttttct ttgaacgcgc cgaacaattc gttgtaacgc ggcaggtgtg
    11821 aagaagaaca agtcggggta aatgcaccgg gcagggagaa tacgactact tttttgcctt
    11881 tgaacaaatc atcggtagac acatctttcc aagtgtcgcc gacgcgggtg cggaatacta
    11941 cggaaggtac tttttgaccg gtacgatctt gcaaagccat tttaagctcc ttaatagatt
    12001 ggtttgagaa ataattgtga atcgctgggc attctacccc atgcaaaatg atttgtataa
    12061 tttatagttc aaataatata aattatttaa aactatcaaa cacaactccg atttgcctgt
    12121 ttcccacata taaagccgaa tgagaaaaaa acaagctgca atgaaaagat tttccggcaa
    12181 ctgcgccctt gcgccgcaaa tcaggacaaa ataccgtctg aaaggctttc agacggtatt
    12241 tattcatacg gtctcggctt atttcgccaa ttcggcacgc agttttcggg ttacgttcat
    12301 catcacttgg agttgttcca gagtttcttt ccagccacgt gttttcaggc cgcagtcagg
    12361 attcacccac agacgctcaa ccggtacaac ctcgatggct ttgcgcaaca ggtgctcaac
    12421 ttcggcttca gtcggtacgc gcgggctgtg gatgtcgtaa acccccgggc cgatgtcgtt
    12481 cgggtatttg aattcgccga acgcggtcaa gagttccata tcggaacgtg aagtttcgat
    12541 ggtgatgacg tcagcatcca tagcggcgat ggccggcaag atgtcgttga actcggagta
    12601 acacatatgg gtgtggatct gagtgctgtc ttcacaaccg gcagaggaca ggcggaaaga
    12661 ttcgcccgcc cagttcaggt aggcatccca atcggcacgt ttcaaaggca gaccttcgcg
    12721 gatggcaggt tcgtcgattt ggatgacttt gatgcctgct ttttccagat ccaatacttc
    12781 gtcgttcaga gccagtgcga tttgtttgca cacggtagcg cggggaatat cgttgcggac
    12841 gaaagaccat tgcagaatgg ttacagggcc ggtcaacata cctttcatcg ggcgtttggt
    12901 caggctttga gcgtaagtag accaagccac ggtcatggct tcaggacggc ttacgtcgcc
    12961 aaagataatc ggtggtttca cgcagcgtga gccgtagctt tgtacccagc cgtattgagt
    13021 gaatgcaaaa ccgctcaaca attcgccgaa gtattcaacc atgtcgttac gctcggcttc
    13081 gccgtgtacc agtacgtcca agtccagttt ttcttgctct tcaaccacca aggcgatttc
    13141 ttttttcatc gcggcttcgt aatcggcggc agacagttcg ccttttttga aggctgagcg
    13201 tgcctggcgg atttcggtgg tttgcgggaa agaaccgatg ttggtcgtcg gcagcagagg
    13261 taggttcaac catgcttgtt gcgctttgat acggtcggca aatggagatt tgcgttggtc
    13321 tgcgttggca ggcaaatcgg ccaggcgttt ggcaacgtct gcacgatgga tttcgctgct
    13381 gttggcacgg gagtcggcag cagcttggct ggcggcgagt tcttcggcaa cagaatcacg
    13441 gccttcgttc aatgcagctt tcagaacgcg caattcttgg tttttttgca gggtgaatgc
    13501 caaccaagag tacaggtcgg gtttgttggc tttcagtttt tcttcaactg acaagtcaaa
    13561 tggagtgtgc agcagcgagc aagagctgga aatccacaaa cggtcaccca gtttggcttg
    13621 cagaggctcg acagtttcca aaactttgtt caggttggcg cgccaaatgt tgcggccgtc
    13681 aatcacgccg gcagacagga ctttgtcgta gtcggcgaac gcgtccagtt gctcgggggc
    13741 gcgtaccaag tcgatgtgca gaccgtcaac aggcagggct ttcaacaatg cggcgtgttc
    13801 ggcaacagaa ccgaagtaag tgctcaacag gattttggcg ttgactttgc tcaaagtagc
    13861 gtaaacgtct ttgtaggctt ccacccattc tttaggcaaa tcgacagcca aagcaggctc
    13921 gtcgatttga atccactcgg caccggcttc aaccaaagca gtcaggattt caacgtaaac
    13981 aggcaacagt ttaggcaaca ggctcagacg gtcgaattcg acggcgcctt tttctttacc
    14041 cacccacagg aaagtcaacg gacctacaac ggtcggtttg gctttcagac cgagggcttg
    14101 ggcttcttgc agttgttgaa catagtgttt ggcattggct ttgaatccgg tatcggcgtg
    14161 gaattcaggc accaagtagt ggtagttggt gtcgaaccat ttggtcattt cgatagcgaa
    14221 ttggtctttg ttaccgcgcg ccagttggaa gaattgttcc aaagacaggt tttggctgtc
    14281 gaagccgaag cgggcgggaa tcgcgccggt ggcgacttgc aggtcgagga tgtggtcgta
    14341 gaaagtgaaa tcgcctacgg caacgaaatc ggcgttggca gcgacctggt gtttccagtt
    14401 tttctcgcgc aagtctttag caacagccag caattcttgc tcgctgattt ctttgcgcca
    14461 gtatttttct tgtgcgaatt tcaattcgcg gaaggcgccg acacgcggga agcctgagaa
    14521 atgtaatgtt gtcatgttga actctccagt tggattttgt aaggaccggt tcaatccgaa
    14581 tcggccggta ttgtaaatca gaatggctcg gatggggcgg tgttttgctt tttagacacc
    14641 ttagatgccg tctgaaactg tttgatacgg ctttaagggc gcacgcctaa aatatggcaa
    14701 atggcgtaag tcagctcgct gcggttaagc gtatagaagt ggaaatcttt cacgccttcg
    14761 cgggacagga ctttgaccat atcgatggcg atacttgccg ccaccagatt gcgcgtacct
    14821 tggtcgtcgt ccaaaccttc atacatttgc gacagccagc ttgggatttt gacgttggtt
    14881 acttgagcca ttttaccgag ctgcttgaag ttggtaacag gcaaaatacc ggggacgatt
    14941 tccacatcga tacccaacat cacgcagcgg tcgcggaagc gcaggtagcg ttccacatcg
    15001 aagaagaatt gggtgatgac gtggttcgcg cccgcatcga ttttgcgttt caaattaatc
    15061 aggtcggctt gtgcggattt cgcttcggga tgcacttcgg gatatgctgc tacagagatg
    15121 tcgaagtcgg cgacggagcg taatagctta accaagtctt cggcgtaaaa cggttttttc
    15181 tcataaccgg ccggctcgtc tccgcgcagg gcgacaatgc ggcggatgcc gctgtcccaa
    15241 taatctttgg caatttggcg caattcgtcg ggagaagcgt cgataccggt caggtgaggc
    15301 gcggcttcca agccggtttc ctgtttgatg cgtttgacga tgccgtgcgt gcggtcgcgt
    15361 tcgccggaat ttgcgccgta agttacggat acgaacttgg gatgcagggt ttgcagacgg
    15421 tggatggagt cccacagcat cgtttccatt tgttcgtttt tcggcgggaa aaattcaaac
    15481 gaaacgttaa tgtcgccttt caaatcagaa agactgttat ttaacgcatt gatttctttt
    15541 gcgtaattca tggctgcgta ccttttatta tcttttatat gatgtttcag gcaacataat
    15601 aaagttgcgc caaacatgag tcaatttcaa aattttcaga tttatcatga aaatttctaa
    15661 tatattacaa aaatagttaa atttccttgt acagcccttc taaactatgc tagagtttgt
    15721 tatgttataa cactgaaagc aaggcaatat atgaaaccaa atattcatcc tgacaattat
    15781 cgaaccgtcc tttttttcga cagcagcgcc aatgaaggct ggttaatccg ctcctgtgcc
    15841 gaaacacacg gaaaaacgat ggtttggaca gacggcaaag aatatccgct gttttcgctg
    15901 gatacttctt ccgcatccca tcccgtctat accggcaagc agcgtaacgt caacaccgaa
    15961 ggacgcgcca gcaagttcaa ccaacgtttc caatccgtca tgtcatcgtt tagaaaggac
    16021 aaataatgca ggttctgtct tcactcaaaa ccgccaaaca acgccaccgc gactgccaaa
    16081 tcgtcagacg caggggcaaa gtttacgtca tctgcaaaag caatccgcgt tttaaagcgc
    16141 gccaacgctg actccctgcc cttcctgctt cggttgtata gtggattaac aaaaatcagg
    16201 acaaggcgac gaagccgcag acagtacaaa tagtacggca aggcgaggca acgccgtact
    16261 ggtttaaatt taatccacta taaagcacct cctaccgcaa gcccttttca tcaaatatgc
    16321 cgtctgaaac agaaaaatgc gtttcagacg gcattttttc cgtcaatacg aatccggaat
    16381 gtgtttgaaa tacagcataa acagatacca aaacagcatc gcggcggctg caccgagcag
    16441 tacccatgcg ctgatttcgg cgacttcgcg cagggcgcgt ttttgcctgc ggctgaaaag
    16501 gcggttgata atcaacgcgg caagtgcgaa aaagaaaaaa atacggaaaa gtctgcctat
    16561 catgtcggga agcggtggag gtcgtctgaa aaagtatgat tcgtgtgcgg cggatgaggg
    16621 tcaaacgcgt ccggctgctt gtccgaccgt gtcaaaaagt catgccgttt gcgatataat
    16681 aacgcttttc gccgcatttg ttggaacagg atatgagtca aaatgcctcg gtgggcattg
    16741 taatgcccca aaaaattccg tttgagatgc cgctggtttt ggaaaacggt aaaactttgc
    16801 cgcgtttcga tctgatgatt gaaacctacg gcgagctgaa tgctgaaaaa aacaatgcgg
    16861 ttttaatctg ccacgcgctg tcgggcaacc atcatgttgc gggcaggcat tcggcggaag
    16921 ataaatatac gggctggtgg gacaatatgg tcggacctgg caaaccgatt gatacggaac
    16981 gttttttcgt ggtcggtttg aataatctgg gcggctgcga cggcagcagc gggcctttgt
    17041 cgattaatcc tgaaacgggc agggaatacg gcgcggattt tccgctggtt acggtgaagg
    17101 actgggtaaa atcccaagcc gcgcttgccg attatctcgg catcgggcaa tgggcggcgg
    17161 ttgtcggcgg cagcttgggc ggcatgcagg ctttgcagtg ggcgatttcc tatcccgaac
    17221 gcgtgcgcca cgccttggtg attgcgtccg cgccgaaact gtccacgcaa aatatcgcgt
    17281 tcaacgatgt ggcgcgtcag gcgattttga ccgatcccga tttcaacgaa ggacattacc
    17341 gcagccgcaa caccgttccc gctcggggct tgcggattgc ccgcatgatg gggcacatca
    17401 cctatcttgc cgaagacggt ttgggcaaaa aattcggacg cgatttgcgt tccaacggct
    17461 atcaatacgg ctatggcgtt gaatttgaag tagaatccta tctgcgctat caaggcgaca
    17521 aattcgtcgg gcggtttgat gccaacacct atttgctgat gaccaaggct ttggactatt
    17581 tcgatccggc ggcggatttc ggcaacagcc tgacccgcgc cgtgcaggat gttcaggcaa
    17641 aattctttgt cgccagcttc agcaccgatt ggcgtttcgc gcccgaacgt tcgcacgaac
    17701 tggtcaaggc cctgattgcc gcccaaaaat ccgtgcagta tatcgaagtc aaatccgcac
    17761 acgggcacga tgccttttta atggaagacg aagcctatat gcgtgcggtc gccgcctata
    17821 tgaacaacgt ttataaggaa tgtcagcaat gaacctgcgc gatgatttgc aactgattta
    17881 cgaccggata cccgaaggca gccgcgtctt ggacttgggc tgcggcgacg gcgaactgct
    17941 tgccgcattg gtcgaacaca aaaaatgcag cggctacggc atcgaaatcg acaccgacag
    18001 cgtcatcgcc gcgatgtcgc gcggcgtaaa tgttatccaa gccgatttgg aagaaggttt
    18061 aaccgcattc aacgatcaaa gttttgatgt gattgtgttg agccaaacca tccaagcgat
    18121 gcagaatacc gaaaaaatcc tgcgctgcct gatgcgcgtt gccaaacagg cgattgtcag
    18181 cttcccgaat ttcggctact ggcgcaaccg cgtccaaatc gcactcggcg gacatatgcc
    18241 ggtttccgaa cgtatgccct accattggta cgacacgccc aatatccatt ggtgcacgct
    18301 caaagatttt gatttgttgt gcgccaaaaa caacatccgc gtcctcgaac gcgccgtgat
    18361 gacgagcaac agacaggtca aacatttccc caaccttttg ggcagcctgg cgttttaccg
    18421 cgtcgggtaa gacaggttga gaaccttgtc ggcaataaca ggttcttcgg ttctattaac
    18481 aacaggccgt ctgaaaaatg aaacggtagg ttttcagacg gcctctttaa tactggaaac
    18541 aatagaatga aaaacaaaac ctcatcactt ctcttatggc ttaccgcaat catgctgacc
    18601 gcgtgttctc cgaacaaaaa cgataaaacc aaagaagtcg gtgcatctgc tgcttcgtcc
    18661 tccgcgtcat cagcttcttc ccaatccgat ttgcaaccgg ccgcatccgc ccctgataac
    18721 gtcaagcagg cagaaagcgc gccgccgtca aattgcaccg acctgcaccc cgccaccggc
    18781 attgacgatc tcatgcagca aatcgccgaa cacatcgact cggactgtct gtttgccctt
    18841 tcccatcacg aactggaaac ccgtttcggc ttacccggcg gcggctatga caacatacag
    18901 cggctgctgt ttcccgacat ccgccctgaa gatcccgact accatcagaa aatcatactg
    18961 gcaattgaag acttgcgtta cggaaagcgc acgatcagcc ggcaggcaca agatgccttg
    19021 atggaacagg aacgccgcct ccgagaagcg acgctgttgc tgatacaggg cagtcaagaa
    19081 acccgcggac aaggcgagga gccgaaacgc acgcgttatt ttgaagtttc ggcaacccct
    19141 gcctattcga gccggcacaa caacggactt ggcggcaatt tccaatacat cggccaattg
    19201 cccggctatc tgaaaataca cggagaaatg cttgaaaacc aatcactctt ccggctgtcc
    19261 aaccgtgaac gcaatcccga caaaccgttt ttagacatcc attttgacga aaatggcaaa
    19321 atcacgcgta ttgtcgttta cgaaaaaaac atctacttca atccaaactt ggggcgaaga
    19381 taaaaaaatg ccgtctgaaa cgttttcaga cggcattttt caatgcaaca aatcaagcgc
    19441 gtttgcggaa ttcgccggtg cgggtatcga tttcgacttt gtcgccgttt tcgatgtaag
    19501 acatcacttg gatttcggtg ccgccgacca ggcgcgcggt tttcatcact ttgccggaag
    19561 tgtcgccttt gacggcaggc tcggtgtact cgacttcgcg cacgatgatg gtgggcagtt
    19621 cgacagaaat ggggttgcct tcgtagaagg taacttcgca ttggtcttcc ataccgtcaa
    19681 cgatgaattt caacgcgtcg ccgatgttgt cggcttcgat ttcgtattgg ttgaattctt
    19741 catccataaa gacgtacatc gggtcggcaa agtagctgta cgtacagttt ttgcgggaca
    19801 ggatgaccac gtcgaatttg tcgtcggctt tgtaaatggt ttcggaagcc gcgccggtca
    19861 gcaggttttt cagtttcatg ctgactttgg cggaagagcg gccgcctttg atgtattcgg
    19921 ttttttgaac gaccatagga tcgttgccga ccataaatac attgccggcg cgcagttctt
    19981 gtgctgtttt cattgtgatt ttccaatcaa atcggttgtc gtaaaacgcg ctattctagc
    20041 gtattttttg atgctttgaa acaaaggcgg cgagtttttc ggatgcggaa ggctgcccga
    20101 aaagataacg gctccaatcc tccgcgcctt gccgccagcc gttttgatgt tgttgcagga
    20161 tttgccaaca ttcgaggcgt tgtgttgcgg ataaagcctc tccgccgttg aggtcgtctg
    20221 aaaggcggcg gtgtgccgat gcggtttcgg gcgtgtagaa accgtgtgcc ttatcccaaa
    20281 aggcgtggag tttgtcgaga tggacattct cgtcttgcgg atagatgtgc caaaagaagg
    20341 gttttccggc aagctgggcg cgcacgaaac tgtcttcgcc gcggatgacg gcgcagtcgg
    20401 caaggtgcag cagtttgtcg aagtcctgtt gcggcacgaa agggattttg acgaggcgga
    20461 cggatgccgt ctgaaaaaca tcgccgtcgt tttgcagggc atcttgcgga ataacgccgc
    20521 tttgtttgag
//
LOCUS       DAWHXZ010000008        20341 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000008 DAWHXZ010000000
VERSION     DAWHXZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20341
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.008"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..553)
                     /locus_tag="WIE35_000210"
     CDS             complement(<1..553)
                     /locus_tag="WIE35_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153196.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDI"
     gene            complement(667..1059)
                     /locus_tag="WIE35_000211"
     CDS             complement(667..1059)
                     /locus_tag="WIE35_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ4153197.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(1267..1659)
                     /locus_tag="WIE35_000212"
     CDS             complement(1267..1659)
                     /locus_tag="WIE35_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ4153198.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(1776..2186)
                     /locus_tag="WIE35_000213"
     CDS             complement(1776..2186)
                     /locus_tag="WIE35_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ4153199.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(2200..2637)
                     /locus_tag="WIE35_000214"
     CDS             complement(2200..2637)
                     /locus_tag="WIE35_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ4153200.1"
                     /translation="MKISIFWLYNAFILKRSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(3052..4350)
                     /locus_tag="WIE35_000215"
     CDS             complement(3052..4350)
                     /locus_tag="WIE35_000215"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ4153201.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDVVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVEACKPIYETMPGWRESTFGVKDYGA
                     LPENAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(4454..5605)
                     /locus_tag="WIE35_000216"
     CDS             complement(4454..5605)
                     /locus_tag="WIE35_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ4153202.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTETVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPTIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            5940..7319
                     /gene="norM"
                     /locus_tag="WIE35_000217"
     CDS             5940..7319
                     /gene="norM"
                     /locus_tag="WIE35_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ4153203.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            7498..8538
                     /gene="murB"
                     /locus_tag="WIE35_000218"
     CDS             7498..8538
                     /gene="murB"
                     /locus_tag="WIE35_000218"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ4153204.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVIGAEKAAALLEQHPDMPHYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDKQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            8550..9200
                     /locus_tag="WIE35_000219"
     CDS             8550..9200
                     /locus_tag="WIE35_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ4153205.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            9255..10022
                     /locus_tag="WIE35_000220"
     CDS             9255..10022
                     /locus_tag="WIE35_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ4153206.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGFPAPDFAPDDSMGKIIRTVCDNS"
     gene            10082..10648
                     /locus_tag="WIE35_000221"
     CDS             10082..10648
                     /locus_tag="WIE35_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ4153207.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            10668..11558
                     /locus_tag="WIE35_000222"
     CDS             10668..11558
                     /locus_tag="WIE35_000222"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ4153208.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            11583..12341
                     /locus_tag="WIE35_000223"
     CDS             11583..12341
                     /locus_tag="WIE35_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ4153209.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITSE
                     NRWESDTSRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(12396..13061)
                     /locus_tag="WIE35_000224"
     CDS             complement(12396..13061)
                     /locus_tag="WIE35_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ4153210.1"
                     /translation="MTVLDREQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(13261..14157)
                     /locus_tag="WIE35_000225"
     CDS             complement(13261..14157)
                     /locus_tag="WIE35_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ4153211.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTVARLTMTVYLPAEQKGTHMS
                     RFVALMEQHSEALDFAQLHRLTAEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEMKDGAYGYSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            14226..15383
                     /locus_tag="WIE35_000226"
     CDS             14226..15383
                     /locus_tag="WIE35_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ4153212.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFTLHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLQPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKATGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPMDCWLVLRGIKTLALRMEAHCKNALEIARRLEDHPAIEKVFH
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(15446..16447)
                     /gene="hemB"
                     /locus_tag="WIE35_000227"
     CDS             complement(15446..16447)
                     /gene="hemB"
                     /locus_tag="WIE35_000227"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ4153213.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAEEAYNPE
                     GLVPAAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIREGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(16630..16914)
                     /gene="yhbY"
                     /locus_tag="WIE35_000228"
     CDS             complement(16630..16914)
                     /gene="yhbY"
                     /locus_tag="WIE35_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ4153214.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            17024..17644
                     /locus_tag="WIE35_000229"
     CDS             17024..17644
                     /locus_tag="WIE35_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ4153215.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINDKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGSSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            17708..19675
                     /gene="ftsH"
                     /locus_tag="WIE35_000230"
     CDS             17708..19675
                     /gene="ftsH"
                     /locus_tag="WIE35_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ4153216.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            19896..20327
                     /locus_tag="WIE35_000231"
     CDS             19896..20327
                     /locus_tag="WIE35_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ4153217.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
BASE COUNT         4894 a         5726 c         5098 g         4623 t
ORIGIN      
        1 ctatatcaga taaaacagcg gtaaaattgc cgtctgaaac caactctgcg ccatacggga
       61 cacgctctgc acgtctattt gcctccgata tatcctccct actgtaggaa cgtctgccga
      121 tttgcacgaa atcggcacct ctagcaccct tcaggatggc ggacgttatc cgttcttggg
      181 attctgcaag acggtcgacg gcattataca gacgctcggt ctgataccct tccgaagcaa
      241 cctgcttcag gtattccaaa taaccgtaac ccaacgcccc tccgacaagc agggtcgtca
      301 acgctaccag cgttgccgaa cggattttag ggggcatatc ttgaagcata tctctaccga
      361 accgaaccaa agtctcttta aagtcggact ctacacgcgt agaaccctcg gaaatcttga
      421 agttcaactc atactgcttc cgtttgtccg ctgtcaaccg cttgaggttg tcatccccgt
      481 attggacggt tgacacaacc ctgtacagac tgtcctgtat ttcgaccagc gtctgtgcaa
      541 agttggcggt cataactgga ttcccaaccc ttgccgccga ttctgatact caaaacggta
      601 tccaggcgtt ccagcagggc ttcgggagac agcagtgcgt attcttcaat attttccata
      661 ggttgattat agcagaacct tatacctgtt acagacaagg ttgtcgggat ttgaacgtcc
      721 gtcgagcttg gttcgtatag agccgtaggg ggtgtttagg gctttggcgg cagcttgtac
      781 gctggggaac gtgagtgttt cgcctgtggc ggtgtcgagg atggagacag gtttttggtt
      841 ttggggaggc gtattttcct tcattttaag tttcccgcac tccggacacc catactttga
      901 ctttatagct gtgtcgtaat acaaaagttg gacaaccccg tgcgtaggac atctaacact
      961 ggaaggttta taaatgccac tgaactccaa caattctaaa tgtggaaatt tctcttgaaa
     1021 gcgtgatttg gcggtatcga aagtcattcg cttacccata atttgtctcc ataggttatg
     1081 tgcaaatgga ttacacaacc aaaatataga aattttcaaa ttttttgaga cctacctgaa
     1141 atcctgcgca agcacaatgt aaagaaaaat ggtatacgtc tttactaaat ctttacataa
     1201 attaactcta atcgggattt gaacgtccgt cgagcttggt tcgtaggatt tagggggttg
     1261 tgaaccttac ctatcgtacc caagcacttt gtacctgttt gaaataaggt tgtcgggcga
     1321 cgttcgaccc ttgagcctgt gattgatttg ttggaaatgt acacctaaag cagcacctgc
     1381 ggcagtaacg cttgggaacg tgagtgtttc gttggtcgtt gtgtcgagga tgaggagggg
     1441 cttccctaca tttggaggtg tttgggtttt tgacatctta gcacactcag gacacccgta
     1501 ctttgacttt ataagggcat taaatgtcga ataacgaacg ttcccgtgca taggacaatt
     1561 aacggttact gggtaattca caccgtcaaa gccggttaga gagatattag ggaatatatc
     1621 agtaagttca gaagctgctt ggttgaaaga tttggccatt tgagactcca aaattgattg
     1681 tcaaatggat ttaatagcaa atttataaaa atgtcaaatt tttcgagaag cacctgatag
     1741 tggcgagggg tatacactgt taaaaagtgt atacgtcatt tagtgcagat gtgaacctta
     1801 tacctccctg caagtaaagt ctctgtgtga acccgacctt ttatacggtc agtgatagct
     1861 gctgggttta tacccaatga ccttgcagca gatgctcttg aggggaatgt aagtgtttcg
     1921 ccagttacag tatcctccaa tactgtattt ttagataatt tatctattgt atcttctgga
     1981 attttgtggg aattgacacc ataagatgca cattttggac acccactttt agagtccaac
     2041 atacttttga atgtagagca agtaaccctc ccgtgtatag gacagacgat taccgtcggg
     2101 taacgaacgc cattgaactc cacaagattc aaatgtggaa atttagaggt tagcttagaa
     2161 actgcttgag taaatgtaag tgccatagtg gttgtgaact tactttacca acctataacg
     2221 atacctaatc aatcggtcaa acggagtgcg tccgcttaaa cgtacagcta aagtgctgtt
     2281 ggagcaaccg ataaatttac tggcagccag tgtacttgca aactcgtaga tttcccctgt
     2341 ctctgtgtcc tccaacctaa tggcaacggc gtttggagag ggcttttcgg gatttcgagt
     2401 acattcagga catcctgttg aggatttcat ggcagcttgt agattcccgt aaacaaccga
     2461 cccatgcaaa gggcattgga ttgttaccgg ttcgtagttt ttagtgaact tcaaaaccgt
     2521 cttatcagga aatttactgg aaaaccttaa ctgaacatct tgtagcgaga gtgattttgg
     2581 cattatagac ctttttaaaa tgaaagcatt atacaaccaa aatatagaaa ttttcaaatt
     2641 ttctctgaac gcactggtat ccgatggagc taaatgtaaa gaaaaagggt atacgtcttt
     2701 actaaatatt tacacatttt aactgtatag tgtttgacaa acaacaggca taggcgcata
     2761 atgcacccgt agttagatag atagatagta aggagataac caatgactta gcacctgccc
     2821 cctctctctc gcgcaccccg cccctcgctt gcggctaatg ggctttgcgc gacatataca
     2881 ctttcatctc ctgcatggct taaccgccta tgcaatagta cacacatcaa caaatcaggc
     2941 tggcacgtct tggcattggc tgacgtacca ttgtagcccg aaatgttgat accctactat
     3001 ttatgaccaa cggcggatta gggcttcaga cggcaatttt actgcaaaac cttatgcgaa
     3061 cggatgatgc agcacaatcg tttcttctcg gtcggggccg gtggagacga tggcgaccgg
     3121 cgcgccgcag acttcttcaa tccgtttcaa atatgctttg gcgttttcgg gcaatgcgcc
     3181 gtagtccttc acgccgaaag tggattcacg ccagccgggc atggtttcgt aaatcggctt
     3241 gcaggcttct accgcatcag agccgcaagg caggatgtcg gttttgccgc cgtcgggcag
     3301 ttcgtagccg acgcagatat tgatggtttc aacgccgtcc attacatcga gtttggtaat
     3361 gcacataccg gaaatgccgt tgatttggat ggagcgtttc agggcggcgg catcaaacca
     3421 gccgcagcgg cgcgcacgac cggttacaga accgaattcg tgtccgcgct ccgccaaacc
     3481 tgcgcctact tcgtcgaaca attcggtcgg gaacgggccc gaaccgacgc gcgtggtata
     3541 ggctttgacg atgcccaaaa cataatccag catttgaggg cctacgcccg cgcctgccga
     3601 agccgcgccg gcgaggcagt tggacgaggt aacgaaggga taagtgccgt agtcgatgtc
     3661 caacaacgca ccttgcgcgc cttcaaacag cagtttttcg ccgtttttgt ttttctcgtt
     3721 caacacgcgg gacacgtcgg taatcatcgg cgcaatgcgc ggcgcgactt tttcgataac
     3781 cgccatcacg tcttccgctt taaccggttc ggcattgtgc agatgttgca gttggacgtt
     3841 gtaataagca aggacgacat ccagtttttc acgcagtttt tcaggatgca gcaaatcggc
     3901 ggcgcgaatg gcgcggcgtg ccactttgtc ttcgtaggca gggccgatgc cgcggccggt
     3961 cgtgccgatt ttgcctttgc cgcgcgatgc ttcgcgggct tggtcgagcg cgatgtgata
     4021 aggcaggatc agcgggcagg tcggcgcgat tttcagacgg ccttcgacgt ttttcacgcc
     4081 tgccgcgttc aactcgtcga tttcgcccaa cagggcttcg ggggagacga caacgcccga
     4141 accgatgaag cagtccaaac tttcatgcag gatgccgctc ggaatcaggc gcaaaatggt
     4201 ttttttgccg ccgacaacca aggtatggcc cgcattgtgg ccgccttgga agcgcaccac
     4261 gccgccggct tcttccgcca gccagtcaac gattttacct ttaccctcgt cgccccactg
     4321 tgcgccgatt actacaacat ttttagccat agccatataa cctatcgata ttaaaaatta
     4381 ttgcggaaat ccccaaaccg gtgcggcttt gcgccccgcc acgatttgcc tttcatccgc
     4441 catgaacggg tatttagcgt ttcacgacct gccaaacgcc gtccgtcttt ttcagacggc
     4501 ctgcaagctc ttccgaaaca ttgtgtccga taccgtagtc aatcacgaca cactgccctt
     4561 gttcacgcaa ggcttcgact gcttcgcgcg ccgcttcggc atcttccgca tcgaccaaca
     4621 cggcgggctg ccgttcgatg gtgggcaaac gcccgataaa gctgcgcaag tcgaaactga
     4681 atcccgttgc cgggcgcgcc ctgccgaaat atccgcccaa tccgtcataa cgcccgccgc
     4741 gcgcgaccgc gtcgtggaaa tcggcggcat aggcggcata cagcaagccc gtgtggtaat
     4801 tgtcgacacg cagctcggac aagtcgatat ggatttcaca atcggggaat gcgtcgcaca
     4861 ccgcctgcaa ttcgcccaac gcgccgccga ccgccgacaa atccggcaac cgtccgcgcg
     4921 cgtcggacaa cacttcacgc ccgccgtaca ggcgcggcag cagcgagaat gcttttgccc
     4981 acataccgtc cagcttccaa gccttgacct gcgcttcgac cgtctcggta tctttatcct
     5041 gcatcaaggc aagcagcgtt gcagactgcc ccgcatccaa atgcgccgca tcggacaagg
     5101 cgcgaaatat gccgatatgc cccagcgaaa gcagcacttt gcccatatcg gcaattttca
     5161 tgcttttcag catcaggtct atcagctcga tgtcgccacg gatgtcggca aaaccgtaca
     5221 tttccgcccc tgcctgcaag ggttcgcgca tattcagcag accgtcggac tgcgcgtgca
     5281 acaccggacc ggcataacac aaccggttaa tcccttggtt ggcggataaa agatgggcat
     5341 cgatacgcgc cacctgcggc gtgatgtcgg cgcgtatgcc caactgcctg ccgctgagcc
     5401 tgtccgttac caaaatggtt ttcagggaaa gccccgcatc gatatgcgtc agcagggaat
     5461 gtgcgtactc catcagcgga ggctgtacca gttcataacc gtgtacgcgg aacagtgcca
     5521 acaactgctc cctcgcgctt tcaagctgcc gcgcgttcgt gggcagtacg tcggcgatat
     5581 gttccggaag ctgccatgtc tgcatgagaa tctgccttct attttatcat gtaaatacaa
     5641 aaggctgccc gatccgcaat cagacggcat ctttgccgca acgcatcacg ccgctgtcaa
     5701 aacggcgatg cttccttccg attcagaatc cgaacagccc ctgtccgacg gctcaatgcc
     5761 gtcaagtcgt taaaaccaaa ctttaccata aaatacacac aatctgaata tacttttata
     5821 ccctgtatcc gccgtctgac ggcacggcat ttttttacta tataatgtgc gccattattt
     5881 ctcatcactt cctgctgccg ttttctgctt cagacggcat ttttgttgat tgaacactta
     5941 tgctgctcga cctcaaccgc ttttcctttt ccgtcttcct gaaagaagtc cgcctgctga
     6001 ccgctcttgc cctgcccatg ctgttggcgc aggtcgcgca ggtgggcatc ggttttgtcg
     6061 ataccgtgat ggcgggcggt gcgggcaagg aagacttggc ggcggtggct ttgggcagca
     6121 gcgcgtttgc cacggtttat attaccttta tgggcattat ggcggcgctg aacccgatga
     6181 ttgcccagct ttacggcgcg ggtaaaaccg acgaagtggg cgaaacggga cggcagggga
     6241 tttggttcgg gctgtttttg ggcgtgttcg gcatggtctt gatgtgggcg gcgattacgc
     6301 cgttccgcaa ctggctgacc ttgagcgatt atgtggaagg cacaatggcg cagtatatgc
     6361 tgttcaccag cttggcgatg ccggcggcaa tggtacaccg cgcactgcac gcctacgcct
     6421 ccagcctgaa ccgcccgcgc ctgattatgt tggtcagctt tgcggcgttt gtgttgaacg
     6481 tgccgctgaa ctatattttc gtttacggca aattcggtat gcccgctttg ggcggcgcag
     6541 gctgcggact ggcgacgatg gcggtgtttt ggttcagcgc gctggcattg tggatttata
     6601 tcgccaagga aaatttcttc cgcccattcg gactgacggc gaaattcggc aaaccggatt
     6661 gggcggtgtt caaacagatt tggaaaatcg gcgcacccat cgggctgtct tattttttgg
     6721 aagccagcgc gttttcgttt atcgtgtttt tgattgcgcc tttcggcgag gattatgtgg
     6781 cggcgcagca ggtcggcatc agtttgtcgg ggattctcta tatgattccg caaagcgtcg
     6841 gctcggcggg gacggtgcgc atcggctttt cgcttgggcg gcgcgaattt tcgcgggcgc
     6901 gttatatttc gggcgtgtca ctggtgttag gatggatgct cgccgtgatt accgtgcttt
     6961 ccttggtatt attccgttcg ccgctggtaa gtatgtacaa caatgatccg gcggttttaa
     7021 gcatcgccgc caccgtctta ctgttcgccg gcttgttcca accggcagac ttcacccaat
     7081 gtatcgcctc ctacgccttg cgcggctaca aagttacaaa ggtgccgatg ttcatccacg
     7141 ccgccgcctt ttggggctgc ggtctgctgc cgggctacct gctcgcctac cgtttcgata
     7201 tgggcattta cggcttctgg acggcattga ttgcctcgct caccatcgcc gccatcgcct
     7261 tggtgtggtg cttggaattg tgcagtaggg agatggtcag atcgcataag gccgtctgaa
     7321 aactccgccg attaaaaata tagtggatta acaaaaacca gtacggcgtt acctcgcctt
     7381 gccgtactat ttgtactgtc tgcggcttcg ttgccttgtc ctgatttttg ttaatccact
     7441 atagaaacga tgggctgaag cccgccactg gcaatactca ttttaaagtt caaacccatg
     7501 caacccatcc gataccgaac cgaccttacc ccctacaaca ctttcggcct gcgcgcccaa
     7561 gcccgagcct ttattgcgct tgaacacgcc gacgggttgc gcgacatcgt ccggctaccc
     7621 gagttcaacc gagatactgt tttatggctg ggcggcggca gcaatatcct tttgatggag
     7681 gactacgccg gtctggtcgt acatatggaa aacaaaggca tacgcgaaat tgctcgttca
     7741 gacggcatgg ttctgattga agcgcaggcg ggcgaaatct ggcacgattt tgtcctgcac
     7801 gccgtcgcgc tgggtttgaa cggtttggaa aacctgagcc tgattccggg tacggtcggc
     7861 gcgtcgcccg tgcagaacat cggcgcatac ggcgtagagg cgaaagatgt gattcacagc
     7921 gtgcgctgct tcgatttgga tacggaaacc tttgtaaccc tttccaatgc cgactgccgc
     7981 ttcgcctacc gcgaaagcct gttcaagcag gaaggtaaag ggcgttatgt gattgtttcg
     8041 gtcgtatttg cattaaaaac gcattttgtg ccgaacttgg gttacggcga tttggcggcc
     8101 gccgttgccg aactgagcgc gggcagggtg ccgacggcga aagatgtttc cgatgcagtg
     8161 tgtgcaatcc gcaacagtaa acttcctaat cctaacgtgc ttggtaatgt cggcagtttc
     8221 tttaaaaatc ccgtcatcgg cgcggaaaaa gctgccgccc tgttggagca gcatcccgat
     8281 atgccgcatt atccgcagcc ggacggttcg gtcaaactcg ccgccggctg gctgatcgac
     8341 caatgtcgtc tgaaaggctt ccaaatcggc ggcgcggcgg tacacgacaa acaggcattg
     8401 gttttggtaa acaaaaacaa cgcctcggca aacgatgtcc ggcagttggc gcaacacatc
     8461 aaatttacag tatttgctcg gtttcaggta gaattgcacg ccgaacccaa ttggctgcct
     8521 gcttcgttca gcctgtaaat ccaaggagta tgcccgtgac ccgccccgcc aaaatcaata
     8581 cttacacacg catcatcgac gccagccttg cgcttttcaa cgaggaaggc gagcgcaaca
     8641 tcagtaccaa ccatatcgcg gcgcatttgg gcatcagtcc gggcaatctc tattaccact
     8701 tccgcaacaa agacgaaatc atcgtccaac tgttcaaacg ttacagcgaa gccctgcttg
     8761 cctacctcaa cgaagccgtg ttgccgtctg atgtggaaga ctccatcaac tatatggcgg
     8821 gcatttacga cgtgatgtgg gaataccgct tcctattcag cgacgtaaac accctgcttg
     8881 cacgcagtgc cgaattgttg ggcgaacaca acaccttcac ccaagccaaa gtctccccgc
     8941 tcttggtcaa cctgctgacc cagctcaacg gtctgaacat catccaagcc gaccaaaccg
     9001 ctatgaacga tctcgccgtc aatatgtgga tggttacgaa atactggttc gacttcgaca
     9061 gctccctgcg cggccgcacc aagctgaccg aagactccaa agcacgcggc atccgccgca
     9121 ccttaagcct ccttcgtcct tatcttttgc ccgaacaccg caagaaatac gaccggaaaa
     9181 tcggcaacgg caacccgtaa acccacagcg tccgaatcag ccttcagacg gcacttaccc
     9241 gacggtatca gcgaatgcgc gcagtccccc cgccccatat ctccatcaca ggcctaggtt
     9301 acctcggcct gccgctggca caaaagtttt accaacacgg cagccgcgtc gccgccataa
     9361 aacgcagcct gacttcggac gacatcaacc tgcccataca tctcgacacc ttcgacctcg
     9421 gcagcaccga tgcctttcag acggcattgt ggcggcacca cgccgacaaa cccgtctggt
     9481 tcttcctgct accgccctcc tcgctgacgc attacgccga caccgtcaaa caatgggcag
     9541 aacttgcccg ggcgtgcaac gtgcaacacc tgattttcac aagcagcacc agcgtttacg
     9601 gcgatacagc gcgcgaatgc gacgaaaccg ccacacccga tccgcaaacc gagtccgccc
     9661 gccaaatcct cgccgccgaa caacacctgc tcgacagcgg catctccaac atcgacatcc
     9721 tgcggctggg cgggctttat tgcgccgaac gccatcccgt cagccgcctt gttcaaaagc
     9781 aaaacatccc gggcggcaac cgccccatca acatcgtcca ccgtaatatc gccgtcgaaa
     9841 gcctgtttca gacggcattt aaccccggcg gcaggcggct gaaaaacatt atcgaaccgc
     9901 gccacccgac acgacgcgaa ttctacacgg aagaagccgc caaactcggt ttcccggctc
     9961 ctgatttcgc acccgacgac agcatgggca aaatcatccg taccgtttgc gataacagct
    10021 gaagcctgta aaataagcgt caacagcaac aaacaggcat tctccgcccc gatcagaaaa
    10081 tatgtccgcc ctcctcccca tcatcaaccg cctgattctg caaagcccgg acagccgctc
    10141 ggaacttgcc gcctttgcag gcaaaacact gaccctgaac attgccgggc tgaaactggc
    10201 gggacgcatc acggaagacg gtttgctctc ggcgggaaac ggctttgcag acaccgaaat
    10261 taccttccgc aacagcgcgg tacagaaaat cctccaaggc ggcgaacccg gggcgggcga
    10321 catcgggctc gaaggcgacc tcatcctcgg catcgcggta ctgtccctgc tcggcagcct
    10381 gcgttcccgc gcatcggacg aattggcacg gattttcggc acgcaggcag acatcggcag
    10441 ccgtgccgcc gacatcggac acggcatcaa acaaatcggc aggaacatcg ccgaacaaat
    10501 cggcggattt tcccgcgaat ccgagtccgc aaacatcggc aacgaagccc ttgccgactg
    10561 cctcgacgaa ataagcagac tgcgcgacgg cgtggaacgc ctcaacgaac gcctcgaccg
    10621 gctcgaacgc gacatttgga tagactaacc ttcagacggc atccgacatg aacagccctt
    10681 ttcacaacat cggcatcgta acccgcccca acacgcccga catccaagac accgcacaca
    10741 cgctgattac ctttttgaag cagcacggct ttaccgtcta tctcgacgaa gtcggcataa
    10801 aggaaggctg catctatacc caagacaccg tcggctgcca tatcgtcaac aagaccgaac
    10861 tggggcaata ctgcgacctg gtcgccgttt taggcggaga cggcaccttt ctctccgtcg
    10921 cccgcgaaat cgccctgcgc gccgttccga ttatcggcat caaccaaggg catttgggct
    10981 tcctgaccca aattccccgc gaatatatga cggacaagct attgcccgtt ttagaaggga
    11041 aataccttgc cgaagagcgc atcctgattg aggccgcact catccgcgaa ggcaaaaccg
    11101 ccgaacgcgc catcgccctc aacgatgccg tcctctcccg tggcggtgcc ggacagatga
    11161 ttgagtttga agtcttcgtc aatcgggaat tcgtctatac ccagcgttcg gacgggctga
    11221 ttgtctccac ccccaccgga tcgaccgcct attcgcttgc cgccggcggc cccatcatgc
    11281 aggcaggatt gcacgccttc acgctcgtcc ccatctgccc gcaatccatg accaaccgcc
    11341 ccatcgccat tccagacacg tccgaaatcg aaatcctcgt tacccaaggc ggcgacgcgc
    11401 gcgtccattt cgacggtcaa acccatatcg acgtgcaaaa cctcgaccgc atcaccatcc
    11461 gccgctaccg aaacccctta cgcatcctac atccgaccga ctaccaatat ttcaaaaccc
    11521 tgcgccaaaa actgcactgg ggcgaacaat tggtctgaac cgacccttac cggaaaacac
    11581 ctatgaatcc aaaaactccc cccgaccata cagaagatgc cgtgcgcctg tccaaacgca
    11641 tggcgcaact ggggctttgt tcgcgccgcg aagccgacgg ctatatcgaa cagggttggg
    11701 taacggtcaa cggcaaaacc gccgtactcg gtcagaaagt ttcgccggca gaccgtatcg
    11761 aactaaacaa gaaagcccac gaacagcagg cggcacgcat taccatcctg ctgaacaaac
    11821 ccgtcggcta tgtcagcgca caagcggaaa aaggctataa atccgccgcc gaactgatta
    11881 cctctgaaaa ccgctgggaa agtgacacca gccgcatccg tttcgatccg aaacacaaaa
    11941 tcggtcttgc ccccgccggc aggctcgata tcgattcagt cggattactg gtgttgacgc
    12001 aggacggccg tatcgccaag cagcttatcg gcgaaaacag cggcagtgaa aaagaatatt
    12061 tggtgcgcgt gcgcggaaaa ttggacgaaa aaggacttgc cttactgaat cacggattga
    12121 gtttggacgg tgagaaactg cgtcccgccc aagtagaatg gcaaaacgaa gaccaactgc
    12181 gcttcgtgtt gaaacagggc aaaaagcggc aaatccgccg tatgtgcgaa ctggtcggac
    12241 tgcgcgtcgt cgggctgaaa cgcatccgca tgggtaaggt caagctcggc aggctgccgc
    12301 ccggcaaatg gcgttatctc gcccccggtg aatcgtttta aataagcatt aaaaaatgcc
    12361 gtctgaaaat ccttgcgttt tcagacggca ttgccttatg cccaaataac ggtttcttcc
    12421 aagggcctgc gcgccttcgt ggcgatttcc tgaacgcggt aaccgaatgt cgcggcgacg
    12481 gacacgcccc attctgccgc atcgaacaaa ccgaactgcc cggacaatac gcgttccata
    12541 tcggcatagt tgaaaccttc caccgggcag gaatcgatac ctgccatcgc cgcacccgtc
    12601 atcatgttgc ccaacgcgat ataggtctgg cggcaacacc agtcaaacaa ggcgcgcgaa
    12661 tcgtccaaaa tcttgatgtc gtcggcttga aacgcctgat acctcgccaa agattttgcc
    12721 atggcatccg gttcggtaac gccgcgccgt ttgaggcttt ccaacataaa cgggctgtcg
    12781 aagcgggcat ttttctttgc caaaaacacc accaaatgac tggcggtatc caaagcatcc
    12841 gccatacccc aagaaaacgg cttgattgcc tgtcggattt cggggttttg aaccacaaca
    12901 aactgccaag gctccgaacc gaccgaactg ggcgacaaac gcccgagttc taaaataaac
    12961 tgaaaatcct cggcactgat tttgcgtgcc gcatcgtaat gccggcatga tttacggttt
    13021 ttaaatgcgg atagaacctg ctctctgtct aataccgtca tagtgcatcc ttttattact
    13081 ggtattgcaa attgatttca aaccgatacg gcactgccgc accatacaat ccgtcctcgc
    13141 catcaagctt catccggact ttacccatct ctctacggct gggaaaacaa agcggcggca
    13201 gactcgggaa taacgcccca atgcctttca gacggcattt gggcatcatt gcaaacgcgc
    13261 ctacgggtag gcgatatagg cataagccga atggttgtgg atagactcga aattctcact
    13321 ctcgacgacg aaactcttga tgcgtttgtc ggcgatcagc gaagtagcga catcacgcac
    13381 catatcttcc acgaatttcg ggttttcgta ggctttttcg gtaacatatt tttcatcggg
    13441 gcgtttgagc aaaccgtaga gttggcagct tgcctgcgcc tccacataat caatgacttc
    13501 ctcgataccg acttctgcat cggcagtcag gctgacggta acatgcgaac gctggttgtg
    13561 cgcgccatat tgggaaattt ctttggaaca cgggcaaagc gaggttacgg gaatcatgac
    13621 cttcatactg tagccgtatg ccccgtcttt catttcgccc gtgagacaga catcataatc
    13681 cagtaaggac cgaataccgg aaaccggcgc ggttttcttg cggaaaaacg gaaaagaaac
    13741 gctgattttg ccggcgcggg aatctaaaag cgcgaccatt tcggcagtca gcctgtgcaa
    13801 ttgtgcaaaa tccaaggctt cagaatgttg ctccatcaat gcgacaaaac gcgacatatg
    13861 cgtccccttc tgctcagcag gcaggtaaac cgtcatggtc agacgggcaa cggtggactg
    13921 gatgccttct gcagtttgca gggtaatcgg aaagcgcagg tctttgatac cgacctgatt
    13981 gatcggcaga ttgcgcaaat ctcggctgga ttgcacgtct gcaatagtgt tcatgagttg
    14041 tttttcctta atattcatgt aattgttgag atgtgtgcat ggtatggcgt ttccgccggg
    14101 gaatataccg tcaatctgca aatgcgagat tatatcactc gcttttagca gctgcattga
    14161 ttgtttctat ttttcagata gtgataaaat cttcgattat tcacatctac ataggcagag
    14221 accaaatgaa attcgccacg aaagccattc attccagcta cgattgcgac gaacacaacc
    14281 gcgcgctgat gccgccgatt tatcaaaaca gtatgtttac gttgcacgag attggcgaaa
    14341 atgtgcctta ccgttattcg cgcctgagca acccgacccg tcagattttg gaagacaccg
    14401 ttgccgattt ggaacacggt gcggcaggct ttgcgttttc cagcggtatg gcggggattg
    14461 atgccgtatg gcgtaccttc ctgcaaccgg gcgataccat cgtcgcggtc gccgatattt
    14521 acggcggcgc gtatgactta ttggtcgatg tttatcaaaa atggggggta aatgttgttt
    14581 ttgccgattt aggcaatccc gacaatttgg acgaattgct caaagcgcac aaggtcaaac
    14641 tggtttggct ggaaacgccg tccaatccgc ttttacgctt ggtagacatc aaagcccttg
    14701 ccgcaaaagc caaagcgacc ggcgcgctgg tcggcatcga caacaccttt gccacgccgt
    14761 atctgcaaca gccgttggat atgggttgcg attttgtgtt ccattccgct accaaatatt
    14821 tgtgcggcca ttccgacgta ttgatgggca tcgtcgttgc caaaaccaaa gagctggctc
    14881 agcctttgca cgatatgatg gtgcataccg gtgcgattgc aggcccaatg gactgctggc
    14941 tggtgttgcg cggcatcaaa acgcttgccc tgcgtatgga ggcacactgc aaaaacgcac
    15001 tcgaaatcgc gcgccgtctg gaagaccatc ctgccattga aaaagtgttc catcccggcc
    15061 tgccgtcgca cgaacattat gaactcgccc aagcgcaaat gcctaaaggc atcggcggcg
    15121 tggtgacggt ttatctcaaa aacgacacgc gcgaagcggc aaacagcgtg attaaaaaca
    15181 tgaaactggt caaaatggct tccagcctcg gcggcgtgga aagtttggtc aaccattgct
    15241 attcccagtc ccacagcggc gtgccgcata atgtgaaaat ggaaatgggc atcaaagtcg
    15301 gcctgctgcg tttctccatc ggcatcgaag acgcggacga tatttggaac gatatttccg
    15361 ccgcactcga tacgactttg taaactgtaa aaatgccgtc tgaaaccatg gtttcagacg
    15421 gcatttcaat taacccggcc gaaaatcaac gcttcaacat ctttgccgcc tcaatagcgt
    15481 aataggtcaa aatcccgtcc gcacccgcac gtttgaatgc cagcaggctt tccaaaacca
    15541 ctttgccgcc gtccagccag ccgttggcaa tcgctgcctg caacatcgcg tattctcccg
    15601 aaacctgata ggcataagtc ggtacgccga actcgtcctt cacgcggcgg acaacgtcca
    15661 aatacggtaa accgggcttc accatcacca tatccgcacc ttcccgaata tcgagcgcca
    15721 cttcattcag cgcctcatcg gtatttgccg gatccatctg gtaggttttc ttgtccgcct
    15781 tgcccaaatt gcccgaactg cccaccgcat cgcggaacgg cccgtaaaac gccgaagcat
    15841 atttggcgga atacgccata atccgcgtgt ggatatgccc ggcatcctcc aacgcctcgc
    15901 gaatcgcacc gatacgcccg tccatcatat cggaaggggc aaccacctgt gcgcccgctt
    15961 cagcgtggca caaagcctgc ttgaccaaaa cctctacggt ttcatcgttc atcacataac
    16021 cgttttcgtc cgtcagcccg tcctgaccgt gaaccgtata aggatcgagc gcgacatccg
    16081 tcataatgcc cagttcggga aacctctcgc gcagggcgcg gacggctgcc ggcacgagtc
    16141 cttcgggatt gtacgcttcc tctgcacgct cggttttgtt tgccgtaacc acgggaaaga
    16201 gtgccaacat cggaataccg agctttaccg cctcttccgc cgtaaacagc agcctgtcca
    16261 aactttgacg cttcacaccc ggcatagaag gcacatcctc ctcgcgcgcc gacccctcca
    16321 atacgaacac cggataaatc aaatcatcgg cggtcagcgt gtgttcgcgc atcaggcggc
    16381 gtgaaaaatc gtccctgcgc atacggcgca tacgcgaagc cggaacattg cggtaaggaa
    16441 actgcataag ccctccaatc atcctgtcaa caattcaaac catacggaag ccgccgcaac
    16501 ttgcagacag catcgggcgg caatcaaaat ccgttttcac cggaaagggt ttcggcccgc
    16561 cgaaatgccg cgccgaagga cggcgtcccg cccattaaag aacaatagca aaacaacagc
    16621 ttcaggctgt cagccttcga tattcttacg ccacaatacc aaaagtttcc cgatatgctg
    16681 aacaggttgc gcgtcaaccg cctcacataa ggcagtgcag atttcgatac gttcggcacg
    16741 gtcgtcgccg aatacgcgca ctttaatcag ctcatgcgcc gtcaatgccg catcggtttc
    16801 cttgatgacc gcgtccgtca gaccctgctg accgaccatc acaacaggat ggagatggtg
    16861 cgcgcgcgct ttcagttcca aaatttcttt ggtgttcaat ttggtatccg tcattttcct
    16921 actgcttgaa taaaaagaat aatgcggcat tgtacgcgat ttggcacgac atcggcaaaa
    16981 ttaacgtaca atacgcggtt tccattccga cccgcgaacc actatggctg tacgttccaa
    17041 atcctcaaaa gcgtggctgc acgaacacgt caacgaccac tacgtccata tggcgcaaaa
    17101 agacggctac cgcgcccgtg ccgcatacaa acttttggaa atcaacgaca aagacaaatt
    17161 aatcaaaccc ggcacggtac ttgccgactt gggcagcgcg ccgggaagct ggtcgcaggt
    17221 tgccgccaag ctgacgggtt cttccggagc agttttcgcc ttggacatcc tgcctatgga
    17281 agccataggg ggcgtctcct tcattcaggg cgacttccgc gagaacgacg tactggcaca
    17341 attcgaaacc ttgttggaca accgcccgct cgaccttgta atttgcgata tggcgcccaa
    17401 tatgtcggga aacgccgtaa gcgatcaggc acgcagcttt tatctgtgcg aactggcttt
    17461 agacttcgcc tcgcaacacc tgaaaaccgg cggcagcttt ttggtcaaag tctttcaggg
    17521 tgcaggctat caggaataca tggcagccat gcgcgaaatt ttcggcacgg tgcagacgcg
    17581 caaacccgaa gcctcgcgca atcgctccag tgagatttat ttattgggca aaaataaacg
    17641 ctgacaatac agacggcgtg ctttacaatc atttccgttt tacacctcaa ttatggagcc
    17701 ttgctaagtg gggaacacct ttaaatcaat ccttgtctgg gtcgccttgg gtatcggcct
    17761 gatggctgcg ttcaacgctt tagacggtaa aaaagaagac aacgggcaaa tcgaatattc
    17821 tcagttcatc caacaggtca acaacggcga agtatccggc gtcaacatcg aaggatccgt
    17881 cgtcagcggc tacctgatta agggcgagcg caccgacaaa agcaccttct tcaccaacgc
    17941 gcctttggac gacaacctga ttaaaacact gctcgacaaa aacgtccgtg taaaagtaac
    18001 gccggaagaa aaaccgagcg cgctggctgc cctgttttac agcctgctgc ccgtcctgct
    18061 gctgattggc gcgtggttct actttatgcg tatgcagacg ggcggcggcg gcaaaggcgg
    18121 cgcattctca ttcggcaaaa gccgcgcccg cctactggac aaagatgcca acaaagtgac
    18181 ctttgccgat gtcgccggct gcgacgaagc caaagaagaa gtgcaggaaa tcgtcgatta
    18241 cctcaaagcg ccgaaccgct atcaaagcct cggcggtcgc gtgccgcgcg gcatcctgct
    18301 ggcgggcagc ccgggtacgg gtaagacgct tttggcaaaa gccattgcag gcgaagccgg
    18361 cgtgccgttc ttcagcattt ccggttccga ctttgtcgaa atgttcgtcg gtgtcggtgc
    18421 gagccgcgtc cgcgatatgt tcgagcaggc gaagaaaaac gctccctgca tcatctttat
    18481 tgatgaaatc gacgcagtcg gccgccaacg cggcgcaggt ttgggcggcg gcaatgatga
    18541 gcgcgagcaa acattaaacc aattgttggt tgaaatggac ggttttgaga gcaatcagac
    18601 tgtaattgtg attgcggcaa ccaaccgccc cgacgtactc gatcctgcgc tgcaacgccc
    18661 cggccgtttc gaccgccaag tggttgtccc cctgccggac atccgggggc gcgaacagat
    18721 tttgaacgtc cattctaaaa aagtgccttt ggacaaatct gtggatttat tgtccctcgc
    18781 gcgcggcacg ccgggttttt ccggcgcgga tttggcgaac ttggtcaacg aagccgccct
    18841 gtttgccggc cgccgcaata aagtcaaagt cgatcagagc gattttgaag acgccaaaga
    18901 caaaatctat atgggtccgg aacgccgcag tatggtgatg cacgaagacg aaaaacgtgc
    18961 gacggcgtat cacgaatccg gacacgcgat tgttgccgaa agcctgccct ttaccgaccc
    19021 cgtccacaaa gtaaccatta tgccgcgcgg acgtgcgctg ggtctgactt ggcagcttcc
    19081 ggagcgcgac cgcatcagta tgtataaaga tcagatgttg agccagctct ccatcctgtt
    19141 cggcggacgg attgccgaag acatcttcgt cggacgcatc tccaccggcg catcaaacga
    19201 ctttgaacgc gcaacccaaa tggcgcgcga aatggtaacg cgctacggca tgagcgacaa
    19261 aatgggcgtg atggtttatg cggaaaacga aggcgaagtc ttcttaggac gcagcgtaac
    19321 ccgttcgcaa aacatttccg agaaaaccca gcaggacatc gatgcggaaa tccgccggat
    19381 tttggacgag cagtatcagg ttgcctacaa aatcctcgat gaaaaccgcg acaagatgga
    19441 aaccatgtgc aaagccctga tggaatggga aaccatcgac cgcgatcagg tactggaaat
    19501 catggcgggc aaacaaccca gtccgcccaa ggattacagc cacaacctgc gcgagaatgc
    19561 ggacgcggcg gaagataacg cgccgcacgc tccgactcgg gaagaaaccg aagcacctgc
    19621 cccggcagac accgcttcga cagagtccga gcagcagcct gaaaacaagg cttaacttcc
    19681 cgaacaaacg gcagcccgca agctgccgtt ttcccatcct gttttcagac ggcattttcc
    19741 gctcaatgcc gtctgaaacg ccaaagccgt atcggttttt gcggatctgc gatatggcgg
    19801 tttaaattga aatcagacga ggcaaggctg taccggttta aaattaaacc gctataatac
    19861 cgcctttcga atacacgccc gcattggtga tgcctgtgaa aaaagttgaa aaaaacatct
    19921 tggtgctgca cggcgcggac aaaatgttcg agctggtcga taaggttgaa gactatccgc
    19981 actttctgcc gtggtacagc aagaccgagg tcatcgggcg tagcggcaac gaactgaagg
    20041 cgcggctgtt tatggactat atgcacgttc gccaatcatt cgccacgcac aaccgcaaca
    20101 ttccgggcag ggaaatccgt atggaactgc tcgagggtcc gttcaaaacc ttacgtggaa
    20161 cgtggaaatt tatcgatttg ggcgacgata tgtgcaaaat cgaattcaat ttggaatacg
    20221 atttttccaa tgccgttttg tccgccttaa tttcccccgt cttcaaccac ctttccgcca
    20281 cgctggtcga agcgttcgtc aaagaggcag accgccgtta tgcttgaaat tgagattgtg
    20341 t
//
LOCUS       DAWHXZ010000009        19800 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000009 DAWHXZ010000000
VERSION     DAWHXZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19800
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.009"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(599..3205)
                     /gene="acnD"
                     /locus_tag="WIE35_000232"
     CDS             complement(599..3205)
                     /gene="acnD"
                     /locus_tag="WIE35_000232"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ4153218.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRLGLKRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKTQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(3283..4071)
                     /locus_tag="WIE35_000233"
     CDS             complement(3283..4071)
                     /locus_tag="WIE35_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ4153219.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLTDERNPLYQMIISMF"
     gene            complement(4232..5386)
                     /gene="prpC"
                     /locus_tag="WIE35_000234"
     CDS             complement(4232..5386)
                     /gene="prpC"
                     /locus_tag="WIE35_000234"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ4153220.1"
                     /translation="MTETTQTPTLKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(5472..6347)
                     /gene="prpB"
                     /locus_tag="WIE35_000235"
     CDS             complement(5472..6347)
                     /gene="prpB"
                     /locus_tag="WIE35_000235"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ4153221.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(6973..8283)
                     /locus_tag="WIE35_000236"
     CDS             complement(6973..8283)
                     /locus_tag="WIE35_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ4153222.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(8422..9600)
                     /gene="ftsZ"
                     /locus_tag="WIE35_000237"
     CDS             complement(8422..9600)
                     /gene="ftsZ"
                     /locus_tag="WIE35_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ4153223.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(9719..10963)
                     /gene="ftsA"
                     /locus_tag="WIE35_000238"
     CDS             complement(9719..10963)
                     /gene="ftsA"
                     /locus_tag="WIE35_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ4153224.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(11049..11777)
                     /locus_tag="WIE35_000239"
     CDS             complement(11049..11777)
                     /locus_tag="WIE35_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ4153225.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(11767..12681)
                     /locus_tag="WIE35_000240"
     CDS             complement(11767..12681)
                     /locus_tag="WIE35_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ4153226.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(12795..14204)
                     /locus_tag="WIE35_000241"
     CDS             complement(12795..14204)
                     /locus_tag="WIE35_000241"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ4153227.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(14359..15426)
                     /gene="murG"
                     /locus_tag="WIE35_000242"
     CDS             complement(14359..15426)
                     /gene="murG"
                     /locus_tag="WIE35_000242"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ4153228.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(15430..16755)
                     /locus_tag="WIE35_000243"
     CDS             complement(15430..16755)
                     /locus_tag="WIE35_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ4153229.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(16881..18218)
                     /locus_tag="WIE35_000244"
     CDS             complement(16881..18218)
                     /locus_tag="WIE35_000244"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ4153230.1"
                     /translation="MTLENKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLSPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATKDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGKKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(18308..18580)
                     /locus_tag="WIE35_000245"
     CDS             complement(18308..18580)
                     /locus_tag="WIE35_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ4153231.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRQDLVLAQEWFGKACQNGDQDGCDIYQRLKAGY"
     gene            complement(18695..19777)
                     /locus_tag="WIE35_000246"
     CDS             complement(18695..19777)
                     /locus_tag="WIE35_000246"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ4153232.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIASLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         4735 a         5479 c         4990 g         4596 t
ORIGIN      
        1 gataaaaagg tttcctcaat tccactttct ggattcccgc ctgcgcggga atgacgattt
       61 acagtatagt ggattaaatt tatagtggat taacaaaaac cagtacagcg ttggctcgcc
      121 ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact
      181 atctgtactg tctgcggctt cgccgccttg tcccgatttt tgttaatcct ctataccatg
      241 tcaattcagc ctttatttcg cgggcaaagc ccacgctaca cccactttcc agaagtacat
      301 taggctgcca ctccgacaaa gccgtttgat tccgcaaact gtcggggttc gccacaatct
      361 acggctactg tgttgtgata cggcagatta ttaaaccccg tcattcccgc gcaggcggga
      421 atccaaattt gtccgcacag aaactcatcg gataaataag gtttcctcaa ttccactttc
      481 tggattcccg cctgcgcggg aatgacgatt tacagtagat gcctgccata tcgagaatta
      541 cgtgaatgag caaaatgttg aacccgaccc acgctacgtc ttgcttttca gacgacctct
      601 aagccgcgtt cccttccaaa aaatcctgtg caaaccgttg caacacgccg ccggcttcat
      661 ataccaatac ttcttctgca gtatcgaggc ggcaggtaac gggaacttcg acggtttctc
      721 cgtttttacg gtgaatcacg agggtcaggt cgcagcgcgg tgtgcgttcg ccgaccacgt
      781 cgtaggtttc cgtaccgtcc agttgcaggg tatggcggtt ggtgtcgggt ttgaactgca
      841 gcggcaacac gcccatgccg ataaggttgg tgcggtggat acgctcgaag ccttcggcaa
      901 caatcgcttc tacgccggcg aggcgtacgc cttttgcagc ccagtcgcgg cttgagcctt
      961 gaccatagtc cgcaccggca atgatgatga gcggctgttt gcggttcata taggtttcga
     1021 tggcttccca catgcgcatg gtttcgcctt cgggttcgac gcgggcgaac gagccttggc
     1081 gcacgctgcc gtcttcgttt ttcaccattt cgttaaacag tttcggattg gcgaaggtag
     1141 cgcgttgggc ggtcaagtgg tcgccgcggt gggttgcgta agagttgaag tcttcttcag
     1201 gcaaacccat tttcgccaaa tactcgcctg cggcactgac ggccaaaatc gcattggacg
     1261 gcgagaggtg gtcggtggtg atgttgtcgg gcaaaatcgc cagcggacgc atacctctta
     1321 atgtgcgttc ccctgccagt gcgccttccc agtaaggtgg gcggcggata taggtggaca
     1381 tcggacgcca gtcgtacagc ggactaggtg ctttttgcgc tgtgccggtg tcgaacatcg
     1441 gtacatacac atcgcggaac tgctgcggtt tcacatattc ggcaacgacg gcatcgattt
     1501 cttcatcggc aggccaaatg tctttcaggc ggatttcctt gccgtctgca acgccgagta
     1561 cgtcgttttc aatatcgaaa cggatactgc ctgccagcgc gtaggcaacg accaacggag
     1621 gcgaagcgag gaaagcctgt ttcgcatacg gatggacacg gccgtcgaag ttgcggttgc
     1681 ctgacagtac ggcggtggcg tacaaatcgc ggtcgatgat ttctttctgg gttttcggat
     1741 ccagcgcgcc actcatgccg ttacaggtgg tgcaggcgaa ggcgacgata ccgaagccga
     1801 gtttttccat ttcgggcagc aggcctgctt ctttcaaata gatttcggct acttttgaac
     1861 ccggggcaaa tgaagatttc acccaaggtt tgcgtttcaa gccgaggcgg ttggcattgc
     1921 gtgccaacag cgcggcggca acgacgttgc gcgggttgga agtgttggtg caactggtaa
     1981 tcgcggcgat gatgaccgcg ccgtcgggca tttgtccgtc cgaaggctct tcgtaaggct
     2041 tcgccagccc tttcgccgcc aaatcggcgg tcgcaaaacg ggcatgcggg ttacttggac
     2101 ctgccatatt gcgcgttacg ctgctcaaat caaatttcaa aacgcgagga taaacggcgg
     2161 ttttcaaggc atctgcccat aagcctgcgg ttttggcgta ggtttccacc aatttcacct
     2221 gcgcgtcgtc gcgtccggtc agtttcaaat aatcaatggt ttgctcatca atagcgaaca
     2281 tcgcggcagt cgcgccgaac tccggcgtca tgttggaaat ggtcgcgcgg tcgccgatag
     2341 acaggcttct cgcgccctcg ccgaagaatt cgacaaacgc cccgaccacg cgctctttgc
     2401 gcagaaactc ggtcagcgcc aacacaatat ccgtcgccgt aatgcccgcc tgccgtttgc
     2461 cggtcagctc cacaccgaca atatcgggca ggcgcatcat ggacgcgcgt cccagcatta
     2521 cggtttccgc ttccaatccg cccacaccca cggaaatcac gcccagcgca tcgacgtgcg
     2581 gcgtgtgcga atccgtgccg acgcaggtat cggggaacgc cacgccgttt ttaacttgaa
     2641 caaccggcga cattttttcc agattgattt ggtgcatgat gccgttgccc gccggaatca
     2701 cgtccacatt ttcaaatgcg gtttttgtcc agttgataaa gtggaaacgg tcttcgttac
     2761 gtctgtcttc gatttcgcgg tttttgcgga aggcatcagg atcgtaaccg ccgcattcaa
     2821 cggccaaaga gtggtcgaca atgagctggg tttgcaccac cggattcact ttggcaggat
     2881 cgccgccttt ttcggcaatc gcatcgcgca gacctgccaa atccaccaac gcggtctgcc
     2941 ccagaatatc gtggcacacc acccgcgccg gataccaagg aaagtcgatt tcctgttttc
     3001 cctcaatcag ctgacccagc cagctttgca gcgtcggcaa atcgactttg tccgcgcggt
     3061 tgaccaaatt ctccgccaaa atgcggctcg tgtaaggcag cttgtcgtaa gagccgggct
     3121 tgatgtcctc acacgccgca cgcgcgtcgt agtattccaa atccgtaccg ggcagcggtt
     3181 tgcggtaacg ttggttggca gccatgtcgg ttctcctggg gatctgtttt tcttgtggtt
     3241 tgaggtttca gccgacgttt tgaaggggtc gtctgaaagg gtttaaaaca tcgaaataat
     3301 catctgatac agcggatttc tctcgtctgt caacaatttc acagccatcg aaatgctgat
     3361 gacaatcagc agcggcttaa tcagcttcga accgaagcgg acggcaaatc tcgcacctaa
     3421 attcgcaccg acaaacgcac cgaccgccat cgttgccgca atcgggaaaa taatcgaacc
     3481 gtgcagcagg aataccgata gcgaaccaag attgcaggca acgttcgcca atttggtgta
     3541 agacatcgcg ttcaacagct tgcagccgag caaaacaata aaggcaatca gaaaaaacga
     3601 gccgacaccc ggtccgaaca caccgtcgta aaaacccaaa agtggtgcaa ccgtcagacc
     3661 gaacagaaaa aaagacattc tggctttgcc ttccttactg ccgtcgagct tgggcgaaaa
     3721 cacaaaatac agcgcgacaa atatcaacaa aaccggcacg accgccagca gaatatcttt
     3781 ggaaaccaag ctgaccgata atgcaccgac cacgccgcct gcaaacgatg ctgccgcaat
     3841 cgggagacct ttcttccaat caatcaaacc tttgcgtgca aaagaaaccg tagccgaaaa
     3901 cgtagcagcg gctgcttgca gcttgttggt ggcaattgcc gacacgggag gaatacctgc
     3961 caacaagagt gcaggcagcg taatcaaacc acccccaccc gcaatcgcat cgataaatcc
     4021 ggcaatcatc gcaaccaaac ccaaagcgag tattatgtat aaatcttcca tgtttcttat
     4081 cctgttactt gcaccaatac aggattatct tcttctatta ggttaaattt ttaaacttat
     4141 ttcagacaac ctttccaatc aggcaaggtc gtctgaaatc ctcagctttg cataccgaaa
     4201 ttaaagaaca aactgctatt gcattcttca attatcgttc ttcaatctcc acaaacgcca
     4261 aatcttcagg gcctgtgtag tttgcgctcg gacggatgat tttgccgtct ttgcgttgct
     4321 caagaacgtg tgcgctccaa ccggttgtac gggaaattac gaacagcggt gtgaacatag
     4381 cggtcggtac gcccaatttt tggtaggaaa cggcagagaa ccagtccaga ttcgggaaca
     4441 tttttttctc ttcccacatc acgctttcca aacgttcggc aatgtcaaag aggcgcatat
     4501 cgccggtttc tttgctcaaa ccgcgtgcca cttctttaat gacaacgttg cgagggtcgg
     4561 aaatggtgta caccggatga ccgaaaccga tcacgatttc tttgcggccg atgcgttcgc
     4621 ggatgtcggc ttcagcttcg tcggcattgc ggtagcgttt ttgaatatcg taagccactt
     4681 cgttcgcgcc gccgtgtttc ggacctttca acgcgccgat tgctccggta atgctggagt
     4741 acatatcaga gcctgtaccg gcgatcacgc gggcggtaaa ggtagaagcg ttgaactcgt
     4801 gttcggcata cagaatcagt gaaacgtgca tggctttgat gtgtgattcg cttgggcgtt
     4861 tgccgtgcaa cagttgcagg aaatgaccgc cgatggtctc ttcgtcgctt tcaacctcaa
     4921 tgcgtttgcc gttgtgcgaa tattgatacc agtacaagag gatgctgccg aggctggcga
     4981 tcagtttgtc ggcgatgtcg cgcgcttcac tttccggatg gctttcacgt tcaggatgaa
     5041 cgcagcccag catggatacg ccggtacgca ttacgtccat cggatgggta tgtgcaggca
     5101 ggctttccaa aactttaatc acacggatag gcaggccgcg catggatttg agcttggttt
     5161 tataagcggc cagctcgaat ttgttgggca gatggccgtg aatcagcagg tgggcgactt
     5221 cttcaaactc gcatttttgt gccaaatcca gaatgtcgta accgcgatag ctcaaatcgt
     5281 tgccggtacg gccaacggta cacaaagcgg tattaccggc cgcaacgcca gaaagcgcaa
     5341 cggatttttt aggtttgagg gtcggggttt gagtagtttc agtcatggta tttctccttt
     5401 gtgtttttat gggtttcggg ttttcagacg accgatgcgg atttgttgaa aggcagtctg
     5461 aaagcggtaa atcatttttg aaacaattta tccagttttt gctcgaaggc atgatagttc
     5521 agatgctcgt acagctcggc acgggtttgc atactgtcca ccaccgccgc ctgagtgcca
     5581 tcgcgcataa tcgcttcgta aacattcaga gcggctttgc ttgctgcacg gaacgatgac
     5641 agcggataca gcaccagcga cacgccgttt tcagccagct cgctttgggt ataaagcgga
     5701 gtggaaccaa actcggtaat gttcgccaac acgggcactt tcaccgcatc tgcaaattgg
     5761 cggtacatgt tcaaatcggt catggcttca gggaaaatca tgtccgcacc ggcttcgaca
     5821 caagcttggg cgcgttcgat agcggcatcc aaaccttcta ccgccagcgc atcggtacgc
     5881 gccataatca cgaagttctc atcaacgcgc gcatctacgg cagctttgat acggtcgacc
     5941 atttcatctt tagatacaat ggctttgttc ggacggtggc cgcagcgttt ttgcgctacc
     6001 tgatcttcga tgtgaaccgc tgcaacaccg gcgcgttcaa agttgcgaat ggtacgggca
     6061 atattgaatg caccgcccca acccacatcg atgtccacca gcagaggcgt atccacgttg
     6121 tccgtaatgc gtcgtgcgtc gatcagcaca tcttccattg tggtaatgcc caaatcaggg
     6181 ataccgcaag aacaggctgc cacgccgccg ccggacagat agatggcttt gaaaccgctt
     6241 tgggtggcca atcgtgcaaa ataagcattg acgcaaccgg cgacggcaag cggattcgat
     6301 tctttcacgg cttggcggaa acgtgctccg gcagagtgtt gactcatcat atttctcctt
     6361 tatagacttt ttttcagtat ggacaggctt ccatcacatt cggacggcaa aacacagcca
     6421 tccgacgcgt cgggcatccc aatccaatca ttaaaaatat atgggaaaaa ttatcttatt
     6481 gatatttaaa acgaatcaaa gaaaaacagc agaccgttcg gaattatgcg gcaaaaccgc
     6541 agacaagaag aaaacaaggg gattattcag aaaaggggaa aacatctgaa ttggtttcat
     6601 agtaatgttc ctttgtagtg ttatgtagtt ttatttttcg ataatctaaa acacaagact
     6661 gaaaatgtca aacattttta aggagactca ttacataatt ttaatatttt attttaattc
     6721 catgattaaa ctagattaat tttgaattat ttgtttcaat aaaattgtca ggaaatcaag
     6781 aaaaattttc gataatcaaa atcctgaaaa cttataaaat attttataca taaaaacaat
     6841 gtattatttt taagcaatcc gatgtatttt attatatatt tgatttttat ataatttaat
     6901 aaaaattaaa tatatcggca aacaaaatgc cgtctgaaaa cctttcagac ggcattttta
     6961 atatcgaatc aatcagccca aataccagaa tttcagtgcc cacaatacgg caacaatcca
     7021 taccataggc ggaacgtctt tggtgcggcg gcataaaagt ttaaccacgg catagctgat
     7081 gaagccgaag gcgatgccgt ctgcaatcga ataggtaaac ggcatgaaga caatggtcag
     7141 gaatgcgggt gcggcttccg tcatatcgtc ccaatcaata tccctcgcac tgcggagcat
     7201 ctgcgtgccg acataaagca gggcgggcgc ggtggcaaaa gcgggaacac ttttcgccaa
     7261 aggtgaaaac atcaggcagg cgagcatcaa tacgccgacg gtaaccgccg tcaggccggt
     7321 ccgtccgcct gccgatacgc ccgccgcgct ttccacataa ggcgtggtgg aagaagtacc
     7381 caaagccgca cctgccacaa tggcggtaga gtctgcaagc agtgcgcgtt tcaggcgggg
     7441 cagcttaccg tccaccagca gcccggcacg gtgggatatg ccgaccagcg ttccggtact
     7501 gtcaaataga tcgaccaaga agaagacgaa aatcacactg accatgctga cggtaaacag
     7561 gccttcaaaa tccatctgca taaaagtcgg cgcaatgctc ggtacttcgc cgatgatgcc
     7621 gtgaaattca ttcaaaccca tcaggctggc aatgacggta atggtcaaga tggtgatgat
     7681 gattgcgcct tgaacgcgga aatgtcccaa tacgaccacc atagcaaaac cgaacaatgc
     7741 caacaacgcg gacggctgat gaatatcgcc caaaccgacc aaggttgccg gattggcaac
     7801 gataatgcct gcgcctttca gggaaatcag tgccaaaaac aaaccgatac cggcagcaat
     7861 cgacattttc aaacccatag gcagtgcgtt gaccagcatt tccctgactt taaaaaagct
     7921 gaacaggata aaaatcagac cggagatgaa caccgcaccc aacgcaacct gccaaggcac
     7981 gcccataccc ttaacgacgg caaaggtgaa ataggcattc agccccatcc ccggtgcgag
     8041 tgcaatcgga tagttgccga caaaacccat aacaaaacag ccgatggcag acgcgataca
     8101 ggtagcgacg aataccgccc ccatatccat gccggtctcg cccaaaatca gagggttgac
     8161 gataacgatg tagcacatcg tcaaaaaagt tgtcaaaccc gccatcaact cggtacgcac
     8221 cgtcgtaccg tttgccttca gcttaaaaat cccgtccaac agtgtttgtt ttgaagtgtc
     8281 catatctgaa agtctgtatc aagtggaaaa atctgaatcc gccgaccgca aaccgtacga
     8341 aattgcaaca tcttgaaaaa catacctccc aaaccggaca aaccgattcg ggaggcagca
     8401 ggtttacggg caaacagcac attatttgtc tgaattgtgt tgacgacgca aaatcgcagg
     8461 gatttcaaag tcgtcaagta cggactgatt gtcgaaatcc gcagcggtaa ggttcatcgt
     8521 gcggataccg cgattggtgc ggatcatacc ttcgacattg tggctttgct cctgtttgga
     8581 cggagcaacc gcttctacct cccttgccgg aacaaaatcg accgcgcctt tttctttcag
     8641 accggtagcg ataatggtaa tccggatggc atcttcgctc atggtctcgt cttcagccgc
     8701 accgaatttg cattccaaat cgggatgcgc gctttggttg acgattttca tgacttcgga
     8761 caactcggac attttcaagc aacccggagc agtcgtaata ttgaccagca caccgcgcgc
     8821 tccgtccaag gttacatcgt ccagcagcgg actggaaatg gcctggtcgg tcgccatacg
     8881 cgcacggtcg ataccttggg cataacccga acccatcata gcgataccgc ggttgctcat
     8941 cacggttttc acgtcggcaa agtcgaggtt gatgatttcg ctcgggcaag ttaccacttc
     9001 ggaaatgcct gcgaccgcat cgcgcaatac attgtcggcg gcacggaagg cttcgcgcat
     9061 cgttacgtct tcacccaatg cagtcatcag tttgtcgttc gggatgatga tcagcgaatc
     9121 gacgtgttct ttcaactgtt ccaaccctgc ctgtgcgaca tggacgcgct taccttcata
     9181 tgcgaacggt cgggtaacca cggcaacggt cagaatgccc aaagacttgg caatctcagc
     9241 aacaaccggc gcggaaccgg taccggtacc gccgcccata ccggtcgtga taaacagcat
     9301 attcgcaccg cgaatggctt cttcaatggc ttcccggtct tcctgggctg ccgcacggcc
     9361 gatatcggga ttcgcgcccg cgcccaaacc gcgtgtcaga ttcgtaccca actggattct
     9421 cttcgccgca tggttttttg ccagagactg cgcatccgta ttggcactga taaactccac
     9481 accgcgcaca ttgttggcaa ccatgttatt gattgcattg caaccgccgc cgcccaagcc
     9541 gattacttta atcaccgcag ggctgactgc cgattctgcc acgtcgtaaa caaattccat
     9601 tcaaaaactc ctgctcgccc cattcagagg acggtttaaa taaattatta ttcattatat
     9661 aagaagtatc ttgctgctgg caaaatactt ctcacctgtc aaacggcaat ccacctgttc
     9721 agaagctgtt ttcaatccac cgtttcaatc ttgccaacaa accgccgccc ccttccctct
     9781 cttgcactgc accgttttcc ggctgcggca agtttccttc cagcttgcat gctgcatgaa
     9841 gcagcccgat ggcggtagaa aagcgcggtg tgcggacgcg gtcggacaaa ccgcccattt
     9901 cttggggcgc accggtgcgt acaggcaaat cgaagatttt ttcggcaaat tccacaatcc
     9961 cggtcatcat ggacacaccg ccggtcagaa cgatacccgc attcagcact tctttgggga
    10021 aacccgattt ttgcagctcg cccagcacta cgccaaaaat ctcctgaata cgtgcgctga
    10081 tgattgctgc caaaacctta ctggaaacct gacgcgatgt ccggtcaccc acgcccggaa
    10141 cttcaatcat ctcacccaag ccttccgtat cgcatgatgc cacgccataa tggattttaa
    10201 tgtactcggc ggcatcgaga ggtgttctca acgatttgga caaatctttg gtaatcagat
    10261 taccaccggc cggaatgacg gacgtatggc ggatggcacc gttcatataa acggcaatat
    10321 cggtcgttcc gccaccaatg tcgatgacgc atacgccgag gtctttttca tcttcagtca
    10381 gcaccgcctg cccgcttgcc aacggctgaa gcatgatctg atcgcttttc aaaccgcacc
    10441 gctcgataca tttttggaca ttctgcactg ccgtacttgc accggtaatg atgtgcaccc
    10501 gcgtatccag acgcacaccg ctcataccga tgggctccct cacgccaagt tgggtgtcaa
    10561 taatgtagtc ttgaaccacg gcatcgagaa ttttttgatc gggcgggata ttgattgcct
    10621 ttgccgtttc aatggcgcga tcgatgtctg cctgcgtgac ttccccatct ttaattttaa
    10681 ccacaccttg cgaattgaga ctgcggatgt ggttgcctgc gatacctgtg gtaacgtgag
    10741 taattttggt atccgccatc agctcggcat cattgaccgc ctgcctgatg gcttggacgg
    10801 tggcatcgat attggttacc atgcccgcgc gcaagccccg tgaaggagcc tgccccaaac
    10861 cgacgatgtt gattttgtcg tcatcttgaa cttccccgat cagtgcgagg actttagacg
    10921 taccgatatc cagtacgctg atgtatcttt gctgctgttc cattgttcgt ctgctcttaa
    10981 aactgattga aatttgcgtc gcaccgtttc agacggcacg gccgtaatct gtccgatacc
    11041 tgttcccact attcttcgga ttctttttcg ggtaaaccgt cggaagcata gcggactgaa
    11101 aatccgtcct tatacctcat atccacatag gataaccgat ttttattttt acgcaacaga
    11161 tgctgccacg cttcggtaaa aagccggagg cgtttcatct cgttttccct tccgagcctg
    11221 acggtgatgc cgttgtccaa aacgacaatc cacgccgaac gtgccgtata ggtcatctct
    11281 ttgatgccca aaccctgttt tgccaaaaca gtcgaaaatt cgtcataacg gcggagcatt
    11341 tcggcagacg ttccttccgc gcctctgaat accggcattc cgggtctgtc caagcgggct
    11401 tcaaaaacat tgccttcgcc gtccaccaag gcatggtcgc cccaacgcgc gaccggcttg
    11461 cgctcggtca ggacgacctc aaccgtgtcg ggaaaacggc ggcgcaccat gaccgacgca
    11521 atccacggat accggcggta ggcctcctgt gcgccattga tgtccgtcct caaaatattc
    11581 ccatggatgt attctttcgc caaactgccc aatgctttct tatcggaata aaccaggttg
    11641 cccttcagcg acacctgctt gacgggcaga tgattcgaat tgtaaaacca aaccagcccg
    11701 gacgcagcaa gcagcatcgc catcatgaca agcagccagc gcgtcagccg ttccatcgct
    11761 tcggcattat cccacatgtg cggtcttcaa aatttcaata cataaatcgg caaaacccac
    11821 gcccgtaacg gcagcggatt tcggtactaa actatggctc gtcataccgg gcagggtgtt
    11881 gatttccaac agatagagtt tgccgtcggt atctttgagg aaatcgacgc gcacgcagcc
    11941 ttccgcaccg attgcctgcg cgccgcgaac cgccagttcg cgcatcaggc tttcttcggc
    12001 ttcggtcaga tcttccgaag gacattgata aatggtgtcg tcgcggttgt acttggcttc
    12061 gtagtcgtaa aactcggtcg cggggatgat gtgtatgccg ggcaggcctt tgccgttcag
    12121 gacggggcag gaatattcgc cgccgccgat aaaccgttcg gcaatgattt cgccctgaag
    12181 gtgtttcaat tcttcgtaaa cgcttttcag acggcctttt tctttgactt ttaccacacc
    12241 gacgctgctg ccttcggccg ccggtttcac aaacatcggc aggcccaatt tttcttcgac
    12301 ggcatcgaaa tcagtgtcgt cgtgcaggac ggcgaactcg ggaacgggca gccccaatgc
    12361 ctgccaaatc agtttgcagc ggtatttgtc catgccgatg gcggaggcgg cgacaccgct
    12421 gccggtatag ggaatgccca acagttccaa cgcgccttga accgccccgt cttcgccgta
    12481 agtaccgtga aggatgttga atgccgtctg aaaaccttgt gccttcaatt cagacaatgg
    12541 ggtttccttg ggatcgaagg cgtatgcgtc tatgcctttg ctttttaaag cgttcaaaat
    12601 ggcggtgccg ctgtccagcg agatttctcg ttcgctggaa aaaccgccca tcaatacggc
    12661 cactttgcca aaattctgca ttgttttttg ttctttcctg attgctttat gcttgttgcc
    12721 agaggtcgtc tgaaacctga tttgcggttt cagacgacct ttatatgatg ttccgtctgt
    12781 caggagggtg tgcctcaaat ctgtttcgac aattccagta gcgcggcggg aacgcggttg
    12841 atgcttcccg cgcccatatt caacacgatg tcgccgtcct gcaaaacgtt caacagcatt
    12901 tcgggcagat cggcaacgtt ttcgcagtaa atcggctcga gtttgcccaa cacgcggatg
    12961 gcgcgggcaa gagcgcggga atcggcggcg gcaatcggct cttcaccggc ggcataaact
    13021 tcggtcagca ccagcgcgtc aacggtattg aggactttgg taaagtcttc aaacaaatcg
    13081 cgcgtgcggg tatagcggtg cggctggaag gcgagtacca aacgtttttc cggatacgcg
    13141 ccgcgtgcgg cggcaagggt cgccgccatt tcgacggggt ggtgtccgta gtcgtccacc
    13201 aagagcgcgg tcccgccgtt tggcaacttg atgtcgccgt atttttggaa gcggcggccg
    13261 acgccttcaa agccgagcaa gcctttttgg atcgcttcaa ccgatgcgcc gacttccagc
    13321 gccacgccga tggctgccaa tgcgttcagc acgttgtgtc tgccgggcat attcagcacg
    13381 acttcaaacg acccctgctc atgtcctttc atttgaacat ggacggtgaa tttcatttgc
    13441 gcgccgacgt tttcgatgtc ggtggcgtag atgtcggcgg tatcgtccaa accgtaagta
    13501 gcataaggtt tgctcacttt gggcaaaatc gcgcggacgt gttcgctgtc aatacacaaa
    13561 aaggctttgc cgtagaaggg catacggtgg atgaaatcga taaacgcctg atgcagcttc
    13621 tcaacactgt gcccgtaggt atccatatgg tcttcgtcga tattggtaac gacggacata
    13681 atcggtgtca ggtgcagaaa ggatgcatcc gactcgtcgg cttcggcaac gatgtattcg
    13741 cctttgccca agcgggcgtt ggtgcctgcg gcgttgagtt tgccgccgat aacgaaagtc
    13801 gggtcaagtc ctgccgcgcc gaggatggag gcggtcaggc tggtggtcgt ggttttgccg
    13861 tgcgtgccgg caatggcgat gccgtcacgg aagcgcatca actccgccaa catcagggcg
    13921 cgcggaataa cgggaatttg ctgctccaac gcagcgacaa cttcgggatt ttcttttttg
    13981 acggcggtag aggtaacgac gacatccgca ccgttaacgt gttctgcggt atggccggga
    14041 taaacttgaa tgcccaggct gcccaaatgc tcggtagcgg catttcgcgc ctgatccgaa
    14101 ccggaaactt taaaacccaa attgtgcaag acttcggcga tgccgctcat gccgacgccg
    14161 ccgataccga caaaatggat gttggtaact cgatttttca tcataatgtt gcgttccggt
    14221 tggtttttag tgcgtaaagg cgttatttta aagggctgac cgtttgcgcg ccatagtttt
    14281 ctgacaaata tatagcggat tgaaataaaa aacatccatg ccgtctgaac ggcttttcag
    14341 acggcatggt tcggcagttt acgccgcaca cgcaatcgcg gcttcggcaa cgtcgtccgc
    14401 actgtgcggc agtgccaaag tacgggcgtt ttctgcccat ttgagacatt tttcgcggtt
    14461 caggctgccg aggatttcgg cgagtttttc cgccgtcaac tgggtttgcg gcaacagcaa
    14521 tcccgcctcc gcctgcacca taaaacgtgc gttggcggtt tgatggtcat caacggcgtg
    14581 aggatacggc actaacaacg cacccaatcc cgccgccgtc aactcggcaa tcgtcagcgc
    14641 gccggcacgg caaatcacca aatcggcatc gcggtaggcg gacaccatgt cggtaataaa
    14701 ttccacacat tcggctttca cgcccagcgc gtcgtaatcc gcctgcaagc tgcccagctt
    14761 gccccgtccc gattggtggt acatctgcgg acgcgcattg tcgggcagca aagccaatgc
    14821 ctgcggtacg gttttgttca aaacgtccgc gcccaaactg ccgccgacca ccaaaatttt
    14881 cagacggcct tcacgccctt ggaagcgttc ggcaggcacg ggcaggttgc taatatcggc
    14941 gcggacgggg ttgccgacca agccgccttc gtggctgaac gctttcggaa aagcgtacaa
    15001 cacccgcttc gcccagcgcg acaggtggcg gttggacaaa cctgccacgg cgttttgctc
    15061 gtgaatcaca atcggcacgc ctaatagctt cgccgccaaa ccgccgggga aggtaacgaa
    15121 gccgccgaag ccgatgacgc actcgacacg gtgtttgcgg ataatccgct gcgcttcgcg
    15181 gacggtttga tacaaagtaa acggcagcat cagcttgcgt ttgatgccgt tgccgcgcac
    15241 gcctttaatc gccagcgttt ccaagcgtat gccgtattgc ggcacgatac gctcttccat
    15301 cgaatccttg ctgcccagcc aaatcacatg atggccgcgc gcgcgcaatg aatccgccac
    15361 cgccagcgcg gggaaaatat gtccgcccgt tccgcccgcc atcagcataa aggttttacc
    15421 gcccatgatt tactccaccc gataaccgcg cattttccgg cggttttcat aatctatacg
    15481 caacagcagc atcatgctga tcagcatgaa aaagactgac gaaccgccat aggacatcaa
    15541 cggcagcgtc agacctttgg tcggcaaagc accgatgttc acaccgatat tgaagaaact
    15601 ttggataccg atccaaatgc cgatacccga agcgatatag gcgttgaaag tcaaacccaa
    15661 atcgcgcgac tgcttgccga tggaaaacgc ccgcaccacc agccagccgt aacagaatat
    15721 cagcacacac ataccgaaga aaccgaattc ttcggcgatg atggcaaaaa taaaatcggt
    15781 atgcgcttcc ggcagaaagc cgcgtttgct caaactcgca cccaaaccca taccgaacca
    15841 ctctccgcgc ccgattgcca tcagagagtg ggtaagctgg tagccggcac cctgagggtc
    15901 tttccacggg tccaaaaatg ccactacccg ctgcacacgg tagggagcgg cggtaatcat
    15961 cagcaccatc ccgcccaaga cgctgcctac caggacgaaa aaatatttcc acggcaatcc
    16021 tgccaaaaac agcattccaa cggcaatgac ggtaatgacg acaaacgaac cgaaatccgg
    16081 ctgtaccatt atcagcacca aaccgaacgc caccagcata atcggcagga tgatcgcccg
    16141 gaaacggccg tacatttcta atgtttcacg acgtgcctgc ggattggtgg cggacatgat
    16201 cagattggcc gtcccccgcc aaatcgactg ccaacccaaa ctttccatgc tgcgcaacac
    16261 ttcttcacgg cgcgtgaaca ggcttgccaa ataaaggatg accgccagct tgaacagctc
    16321 ggtcggctgg aaattcaacg gacccaaagg tatccaacgg gtcgcgccat tgatttcgcg
    16381 cccggcaatc aatacgacta ccagcaacag gccggatagg gcaaaaatcc acggcacaag
    16441 ccgccgccat gtcctcatcc tgcaaagaaa ccataaaaaa ccgctcgcta tcaagccggc
    16501 aacgacgaac cccgcctgtc tggtcaaata gaaaaactga tcgccgcctt ccttcgatgc
    16561 caaatacaca gaagccgaat aaatcatcag caggctgaac gccgtcatca gcaccaccat
    16621 ccacaaaagc ggcgcgtcga atttcctgcc gtcgcgcaca atcggtctgt cgagcagcag
    16681 agtgtggaca ccgtcgccca cttttaccaa tacttccgaa atcttcaaaa aaaccacctg
    16741 ccagtctgtt tgcacctgcc gcaaagggca aaaatttcag acggcagaca atgccgtccg
    16801 aacatacgat acatcccaaa tcggtattct aaatctttac ttgccgccca acaatgacgg
    16861 cgtttgcatt tcagacggca tcacaaagcc ttaaacgctt cgataaacac ttccgaacgg
    16921 tgcgcgtagc ctttaaacat atcaaaactc gcacaagccg ggctgagcag cacgatatcg
    16981 cacgcttcgg cttgggcata tgccgtctga acggcttctt ccaaagtggc gcagtcggtc
    17041 atattcagac cgcagccgtc caaatcgcgg cggatttgcg gcgcatcaac gccgatcagg
    17101 aacacgcctt ttgccttgcc ggcaagcgcg tcgcgcagag gcgtgaagtc ctgccctttg
    17161 cccatgccgc ctaaaatcac gaagagcgga ttttgcaaac cggcaatcgc ggcggcggtc
    17221 gcgccgacat tcgtgccttt gctgtcatca atgaatacca cgccgttttt cttgccgatt
    17281 ttttccacgc ggtgcggcag gccttggaag gttttgacgt gttcaagcaa tgcttcgcgc
    17341 ggcaaaccga cggcttcgca caaagccacg gcagccatga cgttggcggc gttgtgcaag
    17401 ccttgcaacg gaatgtcttt cgtggcaatc aaatcttcat tgccttgttt caggcggcct
    17461 gtctcacgtt ccaaccaaaa atcggcttcg tgttccaacg aaaaccattt tacctcgcgc
    17521 ccggcacgtt tcatggcacg gcagaacgca tcgtccgcat tcaaaacctg cacaccgtcg
    17581 ccacggaaaa tcttggcttt ggtatgcgcg tagtcgagca agtcgtcgta gcggtcgaga
    17641 tggtcttcgg aaatattcag taccgtcgcc gccgtcgggc tcaggctttc ggtgttttcc
    17701 agttggaagc tggaaagctc caacacccac acgtccgcct ttttgccttc gcgctgccat
    17761 tccgcctcca aaaccggcgt gccgatattg cccgcgataa cggtatccag cccgcacttg
    17821 atacagagat agccgaccag gctcgttacc gtggttttgc cgttgctgcc ggtaatcgca
    17881 attaccttgt cgccccggcg gttcacaatg tccgccagca attcgatgtc gcccaacacg
    17941 cgtccgccgt tttgcttgaa cgcctcaata tccggctgac gctcgctgat gccggggctg
    18001 agtgccaaaa tatcgaaacc gttgtccagc gcatctttca gacggcctgt gtaaaacacc
    18061 agcccgtcaa acatcttacc gatttgcgac acgcgctcgg gcttcaactc cgcatcataa
    18121 gcggccacct ccgcgccgtt tttgcgcaga taggcaatca tggaaatacc cgtaccgccg
    18181 agtccggcga cgaggatttt tttgttttca agcgtcattt tggttttgtc ctaaaacaaa
    18241 tcatattggg caggtggggg gatgtccgcc cctgcccaag ccgctttcag acggcatcgc
    18301 gggctgttca ataacctgcc ttcaggcgtt ggtaaatgtc gcagccgtct tggtctccgt
    18361 tttgacaagc cttaccaaac cattcttgag cgagcacaag gtcttggcgc acgccgcgtc
    18421 ctttgtaata cattgcgccc aaattgtatt gggctgctgc atttccctgt gctgccgcct
    18481 gcaagttttc ccgaaaatcc gatacgtcat ccgcccacac cgcttggttc aagcccaagg
    18541 caatcagggc ggcggcaagc catttgactg tctgtttcat ggttttactt ctgttttagt
    18601 ataagccgcc gttaacgata gggctgggcg gattgtcgcc gcaggtttat tgcgcgttca
    18661 aatgccgtct gaaaggtgtt cagacggcat aggttcagcg gattttgagg gtactcaaac
    18721 cgatcaacac caagacgatg gtaataatcc aaaagcggac gacgacttgg gtttctttcc
    18781 agcctttttg ttcgtagtgg tgatggatgg gcgccatcag gaagatgcgt tttttggttt
    18841 tcttatacca gccaacctga agcataacgg atacggcttc tacgacaaat aatccgccca
    18901 taatgacgag gacaaactct tggcggacga tgacggcgac ggtaccgagc gcggcaccca
    18961 atgccaatgc accgacatcg cccataaaga cttgcgcggg ataggcgtta aaccacaaga
    19021 aaccgaggca cgcgccgcac atggcggtac agaaaatcac cacttcgttt gcgccggcaa
    19081 cgtaaggtaa ttgcaggtat tgggcaaatt gtgagtggcc gctggcatag gcgaagatgg
    19141 cgaggccggc ggcaacgagg acgacgggga aggtcgcaag gccgtccaag ccgtcggtga
    19201 ggttgacggc attggatgtg ccgacgatgg tcaggtaaga caacaccaaa aagccgacca
    19261 cgcccagcgg cagggcgatt tgtttgaaga acgggacaat caaaatattg ttggcggaat
    19321 tggcggcaag gtaaaacaat gccaaactgg cgataatggc aacgcttgac tgccacacca
    19381 ttttgaattt ggcggacacg ccgttggggt ctttatagac gactttgcgc cagtcgtcgt
    19441 aaaaaccgag tgcgcccgtg gcgagcaata cgcccaagag aatccagata tacgggtttg
    19501 cccagttgcc ccacaacagg gtggacacgg taatggcggt cagaatcagc gaaccgccca
    19561 tcgtcggcgt gccgtttttg acgaggtggg tttgcggacc gtcggtacgc actgcctgcc
    19621 cgcatttgag cgcggtcagc ctgcgtatcg tccacgggcc gaacatcagg gaaaacgcta
    19681 aggcggtcaa cgccgccatg acggcgcgga atgtggtgta ttgaaaaata ttcagaccgg
    19741 ttaaccagtt gctgaaatgt gcgagccata aaaacatggg gcttcctttt tttgttttgt
//
LOCUS       DAWHXZ010000010        19737 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000010 DAWHXZ010000000
VERSION     DAWHXZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19737
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.010"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(5..220)
                     /gene="nqrM"
                     /locus_tag="WIE35_000247"
     CDS             complement(5..220)
                     /gene="nqrM"
                     /locus_tag="WIE35_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ4153233.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(246..1301)
                     /locus_tag="WIE35_000248"
     CDS             complement(246..1301)
                     /locus_tag="WIE35_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ4153234.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(1454..2671)
                     /gene="nqrF"
                     /locus_tag="WIE35_000249"
     CDS             complement(1454..2671)
                     /gene="nqrF"
                     /locus_tag="WIE35_000249"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ4153235.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVSVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(2685..3278)
                     /gene="nqrE"
                     /locus_tag="WIE35_000250"
     CDS             complement(2685..3278)
                     /gene="nqrE"
                     /locus_tag="WIE35_000250"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ4153236.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(3282..3908)
                     /locus_tag="WIE35_000251"
     CDS             complement(3282..3908)
                     /locus_tag="WIE35_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ4153237.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(3908..4684)
                     /locus_tag="WIE35_000252"
     CDS             complement(3908..4684)
                     /locus_tag="WIE35_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ4153238.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(4677..5909)
                     /locus_tag="WIE35_000253"
     CDS             complement(4677..5909)
                     /locus_tag="WIE35_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ4153239.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGVQAFGALTPDLLQQNIANDWHYTLAD
                     ALGINMSSEASVLGKMLFGAIYFLPIYATVFAVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(5912..7255)
                     /locus_tag="WIE35_000254"
     CDS             complement(5912..7255)
                     /locus_tag="WIE35_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ4153240.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAAPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVVALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(7603..9024)
                     /locus_tag="WIE35_000255"
     CDS             complement(7603..9024)
                     /locus_tag="WIE35_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ4153241.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(9382..9741)
                     /locus_tag="WIE35_000256"
     CDS             complement(9382..9741)
                     /locus_tag="WIE35_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ4153242.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(9738..10343)
                     /locus_tag="WIE35_000257"
     CDS             complement(9738..10343)
                     /locus_tag="WIE35_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ4153243.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGILQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(10390..10872)
                     /locus_tag="WIE35_000258"
     CDS             complement(10390..10872)
                     /locus_tag="WIE35_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ4153244.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      11076..11173
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11384..12484
                     /gene="gcvT"
                     /locus_tag="WIE35_000259"
     CDS             11384..12484
                     /gene="gcvT"
                     /locus_tag="WIE35_000259"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ4153245.1"
                     /translation="MITLKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYCIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVNNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQQA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLDKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEVDVRVLKLPFVRNGQKQFD"
     gene            12645..13031
                     /gene="gcvH"
                     /locus_tag="WIE35_000260"
     CDS             12645..13031
                     /gene="gcvH"
                     /locus_tag="WIE35_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ4153246.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDSYGAGW
                     FFKIKPVNPADYDGLLTAEQYAGEVD"
     gene            13178..14425
                     /locus_tag="WIE35_000261"
     CDS             13178..14425
                     /locus_tag="WIE35_000261"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ4153247.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            14702..15127
                     /locus_tag="WIE35_000262"
                     /pseudo
     CDS             14702..15127
                     /locus_tag="WIE35_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            15133..16362
                     /locus_tag="WIE35_000263"
                     /pseudo
     CDS             15133..16362
                     /locus_tag="WIE35_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            16420..17040
                     /locus_tag="WIE35_000264"
     CDS             16420..17040
                     /locus_tag="WIE35_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ4153248.1"
                     /translation="MTTHHVELRNVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALPPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            17129..17260
                     /locus_tag="WIE35_000265"
                     /pseudo
     CDS             17129..17260
                     /locus_tag="WIE35_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            17257..17751
                     /locus_tag="WIE35_000266"
     CDS             17257..17751
                     /locus_tag="WIE35_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ4153249.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(17839..19500)
                     /locus_tag="WIE35_000267"
     CDS             complement(17839..19500)
                     /locus_tag="WIE35_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ4153250.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSETGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTKKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GVGKDGEPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGMKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMIAAEAVFPLLEHLEEAEGFDSGKEAADYQQRFEQSWLYRELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHYGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
BASE COUNT         4751 a         4969 c         5366 g         4651 t
ORIGIN      
        1 ggcgttaacg gtcaacccgt atgccgcctg cctgtttttc ttcatccagt ttcttttgca
       61 gggtgtcgca aggtgtgtcg cagtcgcaca tttttttcat acccaaggca gtaatgccgc
      121 cgcaactgcc tttgatgctg cgtttggaga aaatatagcc gaccgccata ccgatgatga
      181 cggtcaggaa gatgccgaag gtaaggagca gggttttcat ggtgtttcct aatcggtttg
      241 tatgtttagc ggagcagttt ttcaaattcg gaagacatgg cggtgcggta gccgccttta
      301 tccctgacaa tcaggaaaac agcgagtttt tcgcgctctg ccagctttaa ggcttcggtt
      361 tcgcccaata cgaataatcc tgtggacaag ccgtccgccg tcatcgcact gtctgcgacc
      421 acgctgatgg aggcgaggtt gtggctgatg ggtcgtttgt tgttcgggtt gatgatatgg
      481 gagaggcgtt tgccgttttt atcgacgtgg aaaatacggt aatcgccgga agtggcaagc
      541 gaacggttgt tcagcgggac gataatctgc gtattgccgc cttggacgat attgggctgc
      601 tcgataccga tgcgccacgg ttcgccgcgc gcgtttttgc ctttgccgtg caactcgccg
      661 ccgatttcga ccagataatt ttgaatgccg tatttttcca gttcgcccgc aactttatca
      721 acgccgaagc ctttggcaat cgaagataaa tccaaatagg ccttggggtg ggttttgctc
      781 aaggaagcgt aatctttgcc ttgtttcaaa atgattttgt ctatgcccgt ataagatgcc
      841 gcctgtttga tttgttccgg cgacggttca cgggtaacgg atttgtcggg gccgaatccc
      901 caaaggttga ccaaggggcc gacggttacg tccagcgcgc cgtgtgtcag gcggttcagg
      961 cggacggctt cggcagtaac gtgtgcgaag tcgcttgaaa tgcggagggg cttgccggct
     1021 gtgtgttggt tgaaccggct gatttcggag tcgggctgat aggtggacat ctgccggttg
     1081 acttctttaa gcgcgtcatc gatgcgtttt tgtatttcgg caggtgaggg gagtttgtcc
     1141 cgattatttg aaaggtattt gacggtatag gtcgtgccca tcgtttcgcc ttgcagggta
     1201 acggtttgcg cggtttgttc cgaacaggcg ttcaggaaga tgaaacccag ggcaaatatc
     1261 aagacgcgga taaagttcgg caggcgtgtt tcagacggca tagtgtttga cggttttggc
     1321 aaatggtttg aattatatcg caaaacggcc ggtatgtttc tatgccgatg ccgtctgaag
     1381 ggtgttcgga tggcatcggc atagaaaaag gaagaaaccg aggtttcttc cttttgtatt
     1441 tgaagccgaa tatttaaccg ccgaaatcgt ccaagaggat gttttcgtct tccacgccca
     1501 agtctttgag cattttgatg acggactggt tcataatcgg agggccgcac atataaaatt
     1561 cgcagtcttc cggtgcttcg tggtttttca ggtggttttc gtaaaccacg ttgtgaatga
     1621 agcccgtgta gccgtcccag ttgtcttccg gcagcgggtc ggacagggcg acgtgccacg
     1681 tgaagttcgg gaactctgcc gcgagttggt caaagtcttc gacatagaac atctcgcgtt
     1741 tggaacgtgc gccgtaccag aaggtaatct tacgtttgga gttcaaacgt ttcaactggt
     1801 cgaaaatgtg ggaacgcatc ggagccatac ccgcaccgcc gccgataaat accatttcgg
     1861 catcggtgtc tttggcgaaa aattcgccga acgggccgga aatcgtaact ttgtcgccgg
     1921 gtttgagcga ccagatgtag gacgacattt gtcccggagg cgcatcaggt acgcgcgggg
     1981 gcggcgtggc gatacgcacg ttcagcataa tgatgccttt ttcttcagga tacgaagcca
     2041 tagagtaggc acgcaaaatc ggctcgtcca ctttggaaac gtattgccac aaattgtatt
     2101 tgtcccagtc ttcgtgatat tccttaggaa tgtcgaagtc tttgtaggca acagtgtgag
     2161 gaggagcttc aatttgaatg tagccgccgg cgcggaaggg gacttcttcg ccttcgggaa
     2221 tggcaagctt gagttcttta atgaacgtgg ctttgttatc gttggagatg acggtgcatt
     2281 cccatttttt cacgccgaac acttcttcgg gcacttcgat gtccatgtcg gttttgacgc
     2341 tgacttggca cgacagacgg cagccttcgc gtgcttcgcg tttgctgatg tgggacagct
     2401 cggtcggcag gatgtcgccg ccgccgcttt ttacgacgac gcggcattgt ccgcacgaac
     2461 cgcccccgcc gcaggcggag gggataaaga tgccttcgtt ggcaagcgcg cccaagagtt
     2521 tgccgccggc gggcatcgtc agctcttttt cgccgttgac tttgatggtg atgtcgcctt
     2581 cgctgaccag tttggatttg gcaaatagaa tcatcagtgc caaaaccaaa acgatgacgg
     2641 taaacatcac gatacctaaa ataatctcca taccggtccc tttcttataa ctggatgccg
     2701 gagaacgaca taaacgccat cgccatcagg ccggcggcga taaaggtaat gcccaagcct
     2761 ttgaggcctt tgggagcgtc cgaatatttc attttttcgg taatgcccgc caaagcgaca
     2821 atcgccaaca tccagcccaa gcccgcgccg aagccgtata caacggactc gccgaagttg
     2881 tattcgcgtt gcgccataaa cgatacggcg ccgaaaatcg cgcagttcac ggtaatcagc
     2941 ggcaggtaga tgcccaacgc gttatagagg gcggggacga atttatccaa gaacatttcc
     3001 aaaatctgca ccaaagcggc aatcacgccg atgaaggtga tgaatttcaa aaaggtcaaa
     3061 tccacgcctt cgacaatcgc gccgtctttg agcagcgagt aaacgagttg gttgacaggg
     3121 acggacagcc cgagtacgaa aattaccgcc acacccaaac cgaatgcggt ggatactttt
     3181 ttggataccg ccaaaaacgt gcacataccc aaaaagaagg atagtgccat attttcaatg
     3241 aagacggatt tgatgaagag gctcaaatag tgttccatag cttattcctc cgcctgttcg
     3301 ggtttccagg tacgcagtcc ccaaatcaaa aagccgatga tgaagaacgc gctgggggcg
     3361 agcaggaaca agccgttggt ctgataccag ccgccgtcct gcacggtttg gaaaacggtg
     3421 tagcccaaga gtttgcccga gccgatcagt tcgcggacgg tggcgacgac aagcagcatc
     3481 atcccgtagc ccgcgccgtt gccgatgccg tcgatcaggc tttccagcgg cggctctttc
     3541 atcgcaaatg cttcggcgcg gcccatcacg atacagttgg taataatcag accgacgaat
     3601 acggaaagct gtttggacaa ttcgtaggca aatgcctgca agagttggtc gaccagcgta
     3661 accagcgacg cgataatcgc catttgcacg ataatacgga tgctgttggg gatgtagttg
     3721 cgtaccagcg aaatgaagaa gctggaaaaa ccggttacca aagctacgga aatacccatc
     3781 acgatggccg tctgaagttt ggtggtaacc gccaaagccg aacaaatacc caaaacctgc
     3841 aaggcaatcg ggttgttgtc gataaagggt gaaaacatca aatgtttcaa gcgtttcata
     3901 tcagccatta ttgcgctcct gctgatttca atttgttcag gtaggggata tagccgtttt
     3961 cgccgaacca gtaggcgaac gaaccttgca cgcctttgga tgtcagcgat gcgccggaga
     4021 gggcatctac gccgtgttct ttgtccgaac ccgcgccttt gccgacgtgc agggcgagtt
     4081 tgccttgtcc gtcaaacagt tttttgccga cgaatttttg ctgccacaac ggattgccga
     4141 tttcgccgcc caagcccggg gtttcgcctt gttcgtagta ggtaatgccg ttgatggtgt
     4201 tgccgtcggg ctggatggcg acaaagccgt acatgaccga ccacaaaccg ttaccgtgca
     4261 taggcaggat gatttgcccg attttgccgt cttcgccttt taccaaataa acctcggtgt
     4321 atttggcacg gcttttgatg cctgccaaat cgtcttcggg tttgatgcgg atgctttggg
     4381 cagggtcttt gcctgcgatg cgcgcgctga agtctttagg cgcatcggcg acgtattcgc
     4441 cggtcgccaa atcgacaaca cgttgctcga tacgctcggc aaaggtttta ccgatgtcgg
     4501 tgtccttatc catcaaaccg gctacgctca agatatagcc ttgtttgtct tggagttttt
     4561 gtttctcttg gatgggtttc aagccgacga ccgcaccggc aacgatgacc gagcaaatca
     4621 ggctgaccgc caacacgaca atcagcgtac cgctgaagct gtctttatcg aatttcttag
     4681 ccattgctgc gcgcctttct gcgtttgatg ttcgcttgtg cgacgaaata gtcgaaaatc
     4741 ggggcaaaca ggttggcaaa cagaatcgcc aacatcatgc cttcggggta agccggattg
     4801 accacgcgga ttaatacgca catcacaccg atcagtgcgc cgtaccacca tttgccgaca
     4861 ttggtaaagg aagcggaaac ggggtcggtc gccataaaca gcataccgat ggcgaagccg
     4921 ccgacgacca aatgccagta ccaaggcata gcaaacatag cgttggtgtc cgaaccgatg
     4981 aagttgaaca gcgaagacat cgcaatcata ccgatcatca cgccggcaat aatgcgccaa
     5041 gaagcgatgc gggcaaacac gataaacgcg ccgccgatta agagtgccaa agtggagact
     5101 tcgccgatgg agccgggcag tttgccgata aacgcgtcca tccaagtgat ggtttgaccg
     5161 gttacggcgt ttttcaggcc gtctgcaccg tgtgccgccc attgcgccag cgcggttgcg
     5221 ccggaatagc cgtcaaccgc cgtccaaacc gcatcgccgc tcaagttggc ggggtaggcg
     5281 aagaacagga aggcgcggcc tgccagcgca gggttcataa agtttttacc cgtaccgccg
     5341 aatacttctt tcgcaaccac aacgccgaaa gaaataccca aagccgcctg ccacagcggc
     5401 agcgtgggcg gaacgattaa ggcaaacaga atcgaggtaa caaagaaacc ttcgttgatt
     5461 tcgtgtttgc gcacggtggc gaacaagact tcccagaaac cgccgacggc aaatacggtc
     5521 gcgtaaatcg gcaggaagta aatcgcgccg aacagcattt tgcccaacac gctcgcttca
     5581 gacgacatat tgatgcccaa agcgtcggca agggtgtaat gccagtcgtt ggcgatgttt
     5641 tgttgcagca aatcaggcgt taacgcaccg aatgcctgta cgccgacgtt gtacataccg
     5701 tagaacatgg cagggaacaa agccagccac accaaaatca tcatgcgttt ggagtcgagc
     5761 gcatcgcgga cgtgtgccgc tttgcgcgtt accgcaccgg atgtatagaa aatcgtcgcc
     5821 gcagcttcgt agagggcata ccatttttca tgtttgccgc ctggcaggaa gtgcggttcg
     5881 attttttcca gaaaatgttt caagcccata atcagccttc cttctcaatg gtttccagca
     5941 ccttacgcaa cagcgggccg tattcgtatt tgcccgggca gacgaagctg cacaaagcga
     6001 ggtcttcttc gtccaattcc aagcaaccca atgcctgcgc gctgtcggta tcgccgacga
     6061 ttaaatcgcg caaaagcagg gtaggcagga tgtctagcgg cattacgcgc tcgtaagtac
     6121 caatcggcac catggcgcgg tcgccaccgt tgacggctgt cgtgaacttg aagagtttgt
     6181 ttttcaggaa atgaccgagg gtcgtacgcg tgatcgagta tttgtccggt tgtggcgcaa
     6241 cccagccgaa cagttcttta ctgcggcctt cttcgataac ggaaatctga ttgtggtagc
     6301 gtcccaaata atcgtgcgcg ccttgtgcaa tcgcgccgtt caataccgaa ccggaaatca
     6361 cgcggttgtc tgcgtcaacc aattcgcccg cagtaatttg cgatactttc gcacccaaaa
     6421 cggtacgcaa gaggcgtggt ttgttgactt gagaaccacc caaggcaacc acgcgctcgg
     6481 tgttcagacg gcctgttgca aacaaacgtc cgatggcaat tacatcttga taattgatgg
     6541 tccaaacggt tttgttcgca ccgacaggct caatgaaatg aatgtgcgtg ccactcaaac
     6601 cggcaggatg cgggccgcca aattcatgag tttcgatgtt ggcggcgttt tcagacggca
     6661 cgtctgcgcc tgctgcttta cacacatgga ttttacgctc ggtcaaacgg ctcaatacca
     6721 gcaaacctcg tctgaaatcc tcggcggctt ctttgatcac aaccacaggg tctgccgcca
     6781 gcggattggt gtccatcgca ttgacgaaga tggcgaacgg cgcggcatcg acggcaggga
     6841 ttttgctgaa cggacgggta cgcagcgcag tccacaaacc ggattggatc agattgcggc
     6901 gcacttcttc gccgcttaag tttgccaacg cttcgggtgc gtagcgttca aactcgattt
     6961 cgtcgttgcc ttcaacggca atcacgactg actgaagtac gcgcttttcg ccgcgatgga
     7021 tggcggcgat tttgccagaa gccggcgcgg taaacaccac gcccggattc tttttgtctt
     7081 caaacagcac ttggcctttt ttgacggcat cgccttcctt gactttcatc gaggggcgca
     7141 taccggcata ttcttcgcca agcaacgcga cttcggtaat ggccgggccg tcataaatga
     7201 cttgctccgg tctgcccgcg atgggcaggt ttagaccttt tttgatttta atcatatatt
     7261 tgcattactt gtgatggtta aggtaaaaac ggcgtgtttt gataccgtgt cgcgtggcat
     7321 caaaagcatt gaataaatta atgtagcaaa gtgttagatt ctatcaggaa ttgtacctgt
     7381 ttgtcagatt tgctgctttt ttccttgcgg aagccgtttt tatagtggat taaatttaaa
     7441 ccagtacggc gttgcctcgc cttgccgtac tatttgtact gtctgcggct tcgtcgcctt
     7501 gtcctgattt ttgttaatcc actataattg tcggaagggg ggatattgat ttgattatgc
     7561 cggaatttaa aatgccgtct gaatgttcag acggcatagc gtttacagca gtttgaaaac
     7621 gaaaaagata agggtatgta cgatgaagac gggtgtcagg aaggcgaccg accacatcat
     7681 atagccgaag aaagtcggca tcggtacgcc gcgctgttcg gcaatggcct tgaccatgaa
     7741 gttcggtgcg ttgccgatgt aggtcagtgc gcccatgaat accgaaccca tagaaaccgc
     7801 cagcagcgaa tgaaacaggg tacccgtcat caaggcttgg gcatcgccgc ccgccatatt
     7861 gaaaaaaacg agataagtgg gcgcgttatc caagaatgcc gacaatatgc cgctcatcca
     7921 aaaatacatc acattaatcg gatgacctgc cgtatcgtga accagcgata ccaccccgcc
     7981 cagcgcgcct gcctcgcctg ctttcagaat gctcaggacg ggaaagatgg tgatgaagat
     8041 gccgaggaag agtttgccca cttcggcgat gggttcaaag ttgaattcgt tgcctgcgcg
     8101 gacttgtttg ggcgtgattg ccatagatac ggcggtcaat gcaatcagga tgacatcgcg
     8161 gacgaggttt tgcagggcgt aacggctgcc gaggatttca aatcccgggt gttcgggttt
     8221 ccaaaggccg gacattagaa ccgcgccgac cacgcccgaa agcaggagga agttccattt
     8281 gccgaagatg gcgatttttt cgggtttttc ctgttgtgcc ggcgtatctt gtgcaatgct
     8341 ttcctgtttg aagaaacggt tgtcgatgaa atagaaggcg gtcaacagga cagcggtgct
     8401 gatcaggacg ggggcgaaca tatgtttgac cgtccacatg aaatctacgc ctttgaggaa
     8461 gccgaggaag agtggggggt cgcccaaagg ggtcagaccg ccgccgatgt ttgcaaccaa
     8521 gaaaatgaag aagatgacga tgtgcacgcg gcgggtacgg ttttggttgg ctttcagcag
     8581 cggacgaatc atcagcattg ctgcgccggt cgttcccatg atagaggcaa gtgcagtacc
     8641 gacggcaagc agggcggtgt tgagcttggg tgtgccgttc aagtcgcccc aaaccaaaat
     8701 gccgcctgaa atggtgtaca gggcaagcag cagcaggatg aaggggatgt attcttcaac
     8761 gagtgcgtgt gcgacggtat ggataccggc ggacgcgcca aaaaccaaac tgaacgggat
     8821 gaggaagagc aatgtccaaa aggcggtaat tttgccgtaa tggtgatgcc aggtatgcga
     8881 aaaaaacaag ggacccaatg cgatagacag caaaatcagg gcaaagggca ggccccacag
     8941 caggtttagg tttgcgccgt ccaaatctgc ggcgtaaacc gatgctggga aaagcattag
     9001 tgaaaacagg ggtaggtggc gcatcgtgtt tcctcgattc aagcactgcc ttgcgcggcg
     9061 cgtgggagtg atacaggcac cgtgccgccc ggacataggc ggactgtgct gcctgtttgt
     9121 ttttgaaaaa agttttccga ttgattgtaa agtaaataat caggataaac cagtttccca
     9181 aaccggaagg cggcgggaag gcggattgcc gtgcttggga atatatgttc tttttgataa
     9241 ataattttat ttaaaacaaa taaatatatg tctttaataa ataatctatc gaaaacgaaa
     9301 aatgaattta ttttaacata tatttgcaat gaaacaggtt tgccccccgt ttgtttgccc
     9361 ttatcccttt cagtacggca ttcaagattc gggcctgcgc cacatccata tggcgacaag
     9421 ggaacaaaaa accgatgaaa ccgccccgac ccaccagcgt tggggaaact gccaaaacat
     9481 tatcaggcag gatgcggtca tcatactgat ggcgaatatt ttggctttgc gcggcactgc
     9541 gccgttttgt tcccagttat gaaccatcgg accgaaatag cggtgccggt gcagccagcg
     9601 gtgaaagcgc ggggatgcct ttgcccagca ggcggcggag agcagtacga acggcgtggt
     9661 cggcaacagc ggcaaaaaaa tgccgatgat acccaacagc agggaaatgc agccgcaggc
     9721 aattaaaaga taacgtatca ttttgaaata tttttcttat tgtgcggata agggcaggat
     9781 gtgataccga gttttgccca gccttcatgt cccatttttt ccagcagggc gatattgcgt
     9841 tcgaatattg ccgaagcgtc gggaaaggct tgtgcggctt tggcaatgct gtcttcgcgg
     9901 atcagatgca gtgtcggata gggagaacgg ttggtgtagt tgccgatgtc gtcgggttcg
     9961 gtattctgaa agcggaattg gggatggaac ggtgcgattt ggaggatgcc ttccaagccg
    10021 ttttcgacaa cggcggcatc ggcaatatcg agcatatcgt tgaatacgtc gaaatcgggg
    10081 aatagggtcg ggtgaaccag cagggtggtt tccagttcgg tggcgggtgt attgcccagt
    10141 cgctgcagtt cttcgtccaa gtcttccaaa aaaccgtcaa ggtgtttggc ttcgctgatc
    10201 gcgatgcgga caaggttttt aacgtggggg gctttggcaa agggacacag gttcagaccg
    10261 atgacggctt tttccaacca ttgtccggtg tgttcggcaa cagcatcttt attttcggaa
    10321 gtattgatat tcattattgt catgtaaatg tgtttgcaga ttgcacgtgc gggaaaatcg
    10381 ggaagggcac tattccttca gcaggtggtt gagtggcagg gaggtggtgt gtttgatttc
    10441 ttttaaaaca aagctcgatt gggcatcctg tacgccgtgg tgggacagga gcgtatccaa
    10501 aacaaaatgg gaaaacgcgt tcatatcggt aaaaaacgcc tgaagcaggt agtcggtttc
    10561 ccctgtcagg gcgaagcagc tcaagacttc aggccatttt cgaaccgatg cggcaaagtc
    10621 ttcccgcgcg tcttttgctt tgcggatgga aacgcggata aatgcctgaa gccccaggtt
    10681 gacggattcc ggagacagca gcgcggcata ttggcggacg atgccggcat cttccaactg
    10741 cttcagacgg cgcaggcacg gagaaggcga aagtgcgaca cgttcggaca gttcgacatt
    10801 ggtcagcctg ccgttttcct ggagaacctg taagatttta atatcggttt tgtctaaagt
    10861 gagttggggc atatttgcgt tccgttttaa ggaattcgga ttgtctgtcc gtatgtttgc
    10921 ggcaatccgc acagatggag accatattaa catataaaaa gttataccgt catccgggac
    10981 aaattttgtt ttcggaaaat catgtgaaaa cagaggcggt cggtttgcat ctcttttaag
    11041 acggcttgcc caaaccgccg attcaagaca taatcgggaa atgtgcagga gagtgttaca
    11101 cccaactaca atgtaaccac cgaaggcgca gacaccctta aatcgctcag gtatcaggga
    11161 ctgcacattg aaacaaacaa tctggagagc ggcgttggaa taacgtccac cgaaggggag
    11221 aaggccgtct gaaccaccat tcagacaacc gcgcaaagca gtgagcagac tggtttgcca
    11281 tcatgcggat acggccgaaa atctcaggtt caaggacaga tagggtcatc cgcgcacagg
    11341 tgcgcgggtg gcatctgaac aaaaaatccg gagaaacttg agaatgatta ctctgaaaac
    11401 caccccattt catcaagccc atcaagatgc aggcgcgaag ctggtagatt ttgccggctg
    11461 ggagctgccc atccattatg gttcgcaaat cgccgaacac gaagccgtgc gcaccgacgc
    11521 aggtatgttt gacgtatccc atatgcttgt taccgacgta gcaggcgcaa atgccaaagc
    11581 ctttttccgc aaattgattg ccaacgatgt cgccaagctc gcttttgtcg gcaaagccct
    11641 ttattccgct ttgctcaacg acaacggcgg cgtgattgac gacttgatcg tttaccgcac
    11701 caatgaagcc gaaacccaat actgcatcgt gtccaacggc gcgactcgcg aaaaagatac
    11761 ggcacaattc cacaaagtcg gacaagagtt cggcgtttct ttcaatccgc gctacgacct
    11821 cggcatgctc gccgtacaag gcccgaaagc catcgagaaa ctcctgaccg tcaaacccga
    11881 atgggcagat gtcgtcaaca acctcaaacc gttccaaggc gcggatttgg gcaacgactg
    11941 gtttgtcgcc cgcaccggct acaccggcga agacggcgtc gaagtcatcc tgcccggcac
    12001 cgaagccgtc gcattcttca aagccctgca acaagccggc gtacaaccct gcggcctcgg
    12061 cgcgcgcgat accctgcgca tggaagccgg tatgaacctc tacggcaacg atatggacga
    12121 cgacaccagc ccgctcgaag caggcatggg ttggacggtt gatttgaaag acgaaagccg
    12181 cgattttgtc ggtaaagccg ccttgctggc attgaaagaa aaaggcgttg ccgtcaaaca
    12241 ggtcggtctg ttgctcgaca aaggcggcat cctgcgcgcg catatggaag tgttgaccga
    12301 caaaggcaaa ggcgaaacca ccagcggcgt attctctcca agtctgaaac aatccatcgc
    12361 catcgcccgc gtgccgaaag actttgacgg cgataccgcc aaagttctga ttcgcggcaa
    12421 agaagtggac gtacgcgtac tgaagctgcc gtttgtgcgc aacggacaga aacagtttga
    12481 ttgatgcggt ttcagacggc attttcattt catatgccgt ctgaaagcag gttttaattg
    12541 ttgtccgata cggacgtttg tagaaagcat tgaacaaggc atctgtggat attgattcat
    12601 gcagatgccg tctgaaaata acccctatca atgagtatca aaccatgagc aacaacatcc
    12661 cggccgaact gaaatacgtt gccagccatg aatggctgcg ccttgaagaa gacggtacga
    12721 ttaccgtcgg cattacccac cacgcgcaag agctgttggg cgacatcgtg ttcgtcgagc
    12781 tgcccgaagt cggcgcgaac cttgccgctg aagagcaagc cggtgtggtt gagtctgtaa
    12841 aagccgcgtc cgacgtgtac gcaccgattg caggcgaagt cgttgccgtc aacgaagatt
    12901 tgccaagtgc tccggaaact gccaacagcg attcttacgg cgcaggctgg ttcttcaaaa
    12961 tcaaacctgt caatcctgcc gattacgacg gtctgctgac tgccgaacaa tacgcgggcg
    13021 aagtggacta aatctctatc ctgataatgg gatgactgat ttgagaaact gcactttcag
    13081 acggcaagga tgcgtttgga agtgcagttt ttgtctgtgt cttcaaagtt cagttaggct
    13141 ataataacgg tctatattca tctttaccga ttttttcatg caacttaccg ctgtcggact
    13201 caatcatcaa accgcacctt taagcatacg ggaaaagctg gcgtttgccg ccgccgccct
    13261 gcctaaagcc gtccgcaatc ttgcccgaag caatgcggca acggaggcgg taatcctttc
    13321 tacctgcaac cgcaccgagc tttactgcgt cggtgattcg gaagaaatca tccgatggct
    13381 tgccgattac cacagtttgc cgattgaaga aatccgtccg tatctgtacg cgctggatat
    13441 gcaggaaacc gtgcgccatg ccttccgcgt tgcctgcggc ttggattcga tggtgttggg
    13501 cgagccgcag attttaggac agattaagga tgcggtcagg gttgctcaag agcaggaaag
    13561 tatgggtaag aaactcaatg ccctgttcca aaaaaccttt tctgttgcta aagaggtccg
    13621 taccgatact gccgtcggcg aaaactcggt ttccatggct tccgcttccg tcaaattggc
    13681 ggaacagatt tttcccgaca tcggcgattt gaatgtcttg tttatcggcg caggcgaaat
    13741 gattgagctg gttgccactt attttgccgc caaaagtccc cggctgatga cggttgccaa
    13801 ccggacgctg gcgcgtgcac aggagttgtg cgacaagctc ggtgtcaacg ccgaaccgtg
    13861 cctgctgtcc gatctgcctg ccattctgca cgattacgac gtagtggttt cttcaacggc
    13921 aagccagttg cccattgtcg gcaaaggcat ggtggagcgt gcattgaaac aaaggcagag
    13981 tatgccgttg ttcatgcttg atttggcagt gccgcgtgac attgaagcgg aagtcggcga
    14041 tttgaatgat gcctatcttt atacggtgga cgatatggtc aacatcgtcc aaagcggcaa
    14101 ggaggcaagg cagaaggccg ccgccgccgc cgaaacgctg gtatccgaga aggttgccga
    14161 atttgtcagg cagcagcagg gcaggcagag cgtccccttg attaaagcct tgcgggacga
    14221 gggcgagaaa gcgcgcaagc aggtgttgga aaatgcgatg aaacagcttg ccaaaggcgc
    14281 aacggcagaa gaggttttgg aacggctgtc cgtccaactg accaacaagc tgctgcattc
    14341 gccgacccaa actttgaata aggcggggga agaagataaa gatttggttc atgccgtcgc
    14401 gcagatttat catttggaca aataacggtg cgccggaaaa ggatgccgtc tgaaggggtt
    14461 tcagacggca tatttttgtg ctgtctgaca acatcgtgaa atcccacatt atatcgatgt
    14521 aatcacaaag tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
    14581 cagatagtac ggcaaggcga ggcaacgctg tactggttta aatttaatcc actatattat
    14641 cccgtatgcg gattggtttt aagatttgta aatttgattt gcatcaaaaa atcgccgata
    14701 gatgattcat ataatatcaa tattaaagag tatcggtata tcggggatag tcatgtcctg
    14761 tttttcaatc aaacgtatgt ccgcgtttcg ggcgcggata acggcgtttt ttgccgcctt
    14821 tgtctttttg acggcggcac tgcccgctta tgcggagcgt ctgcctgatt ttctggcgaa
    14881 aatacagcct tcggaaattt ttccgggtgc ggaccgttac ggcaagccgg aaggtaagcc
    14941 tatggttgcc cgcgtttaca aaggcgatga gcagctcggt ttggtctata tcacaaccga
    15001 tgtggtcaat acgcgcggtt attcgagcaa accgattgat acgctgatgg cgttggctaa
    15061 agacggtacg atagccggag cgaaattggt tgatcaccat gagtcgatta tgctgatcgg
    15121 tatcccgcat ttgcccgcgc ccgggcgggc gatacgctca aactggcttc cggcgtatat
    15181 caaaccaaac ttcacatcga caaaccgatt acgattgaag ggcctgccga ccgttctgca
    15241 accatcgaag gagacaggag cgggcgtacc atagccgtac acgcgccgga cgtaacgctc
    15301 cgcaacctga ccgttacccg ttccggtatg agcctgcccg caatggatgc cggtatttat
    15361 ctcgaagaaa ctgccccgcg cgccctgatt gaacacaata atattttgga taattcggtc
    15421 ggcgtctatc tgcatggttc tgccgatgcg atggtgcggg agaataaaat cgtcggcgac
    15481 gcgactttgc gcgtgaacga gcgcggcaat ggcgttaccg tttggaacgc gcccggcgcg
    15541 caggtcgtcg gcaacgatat ttccaaaggg cgggacggca ttttttccaa taccagcacg
    15601 cacaacacct ataaaaacaa ccgcttcagc gatttgcgtt tcgccgtcca ctatatgtac
    15661 accaacgaca gcgaaatcag cggcaatatt tccgtgggca acaatatggg ctatgtgctg
    15721 atgttttccg agcggctcaa agtgtttgac aatatcgccg tcggcagccg cgaccaaggc
    15781 atcatgctca actatgtcaa ctattccgat attcacgaca acattatcaa caaagcgggc
    15841 aagtgcgttt ttgcctacaa tgccaactac gataaactgt ccgccaatca ttttgaaaac
    15901 tgccaaatcg gcatacactt taccgccgcc atcgaaggca cgtcactgca cgacaattcc
    15961 tttatcaaca acgaaagcca ggtcaaatac gtcagcacgc gctttctcga ctggagcgag
    16021 ggcggacacg gcaactattg gagcgacaac agcgcgttcg atttgaacgg cgacggcttc
    16081 ggagacagcg cgtaccgtcc caacggcatc atcgaccaaa tcatctggcg cgcacccgta
    16141 tcgcgcctct tgatgaacag tcccgcaatc agcatcgtca aatgggcgca ggcgcagttt
    16201 cccgccgttc tgcctggcgg cgtggtggac agcaaaccgc tgatgaagcc ttatgccccc
    16261 aaaattcaaa cccgttatca ggcgatgaag gacgggctgc tcaaagaagc cgaaacgcgg
    16321 cagttggaat ggggcagggc ggaaaacggt tctttgaact agtctgtttc agacggcatc
    16381 cggattcaaa tgccgtctga aaacacaaaa ggaaaaacca tgaccacaca tcatgtcgaa
    16441 ttgaggaatg taaccaaacg gttcggggcg caaaaagcgg tcaaccaagt tgatttggtt
    16501 ttgaaggcag gagaaagcgt cgggcttgcc ggacacaacg gcgcgggcaa gtccaccatt
    16561 atgaagctga tactcgggct gattaccccg accgaaggcg aagtgatgct tttgggcgaa
    16621 cgtaccggta gcaaagcggg ggcgcggctt cgcagccaaa tcggctacct gcccgaaacc
    16681 gttgcgctgc ccccttcgct gaccggcatc gaaacgctgg atttttatgc caaacttaaa
    16741 aaacagccgc tcacgcagaa ccgggggctg cttgagcgtg tcggtatttc acaggcggca
    16801 caccgccgcg tcggcactta ttctaaaggg atgcgccaac gccttgcctt ggcacaagcc
    16861 ctgctgggcg agcccaaagt cctgctgttt gacgaaccga caaccggcct cgaccctgca
    16921 tcacgacaaa tgttttacga agtcgtgcgc gaactcaacg ggcgcggcgc gaccgtattg
    16981 ctcagcaccc acgcccttgc cgagttggac ggacacgccg accgcattat cgtggattaa
    17041 atttaaacca gtacggcgtt acctcgcctt gccgtactat ttgtactgtc tgcggcttcg
    17101 ttgccttgtc ctgatttttg ttaatccact atatgcgggt atggcgggtt tgagcggaca
    17161 aatcagcctg accgtccccg ttttgctgac cgctcaggtt ttatgggtta tcattccgct
    17221 tgttttggca gccggaattt ttagaaagcg acaaatatga aaaaaaccct gttggcaatt
    17281 gttgccgttt ccgccttaag tgcctgccgg caggcggaag agggaccgcc gcctttaccc
    17341 cggcagatta gcgaccgttc ggtcggacac tattgcagta tgaacctgac cgaacacaac
    17401 ggccccaaag cccagatttt cttgaacggc aaacccgatc agcccgtttg gttctccacc
    17461 atcaagcaga tgttcggcta taccaagctg cccgaagagc ctaaaggcat ccgcgtgatt
    17521 tacgttaccg atatgggcaa tgttaccgat tggacgaatc ccaatgccga cacggagtgg
    17581 atggatgcga aaaaagcctt ttacgtcatc gacagcggct ttatcggcgg tatgggtgcg
    17641 gaagacgcgc tgccgttcgg caacaaggaa caggctgaaa aatttgcaaa ggataaaggc
    17701 ggtaaggttg tcggtttcga cgatatgcct gatacctata ttttcaaata atattgaaaa
    17761 aaccggccga aactttaaaa acgagtttcg gtcggttttt cttttcttgt tcggtatagt
    17821 gtcggcagga aagaaccttc acatcccgcc gtaattcggc ccgctcgcgc cttcggggca
    17881 aatccaagtg atgttttgcg tcgggtcttt gatgtcgcag gttttgcagt gcacgcagtt
    17941 tgccgcgttg atttgcaggc gcggattgcc gttttcttca acaatttcgt acacgccggc
    18001 cggacaatag cgcgtttcgg gcgaggcgta ttctttgtag ttcacgtcta tcatcgtttg
    18061 cggattgttc agcaccaaat ggtcgggctg gttttcttcg tgcgcgagat tggcaaggaa
    18121 gacgctgctc aagcggtcga aggtcaacac gccgtcgggt ttcggataat caatcggttt
    18181 gcacgcggcg gcttttttga gctgctcgtt gtctttgccg taatgtttca aggtccacgg
    18241 ggctttgcct ctgaaaatca tctggtcgat gccggtatag attgagccga ggtaaacgcc
    18301 ccatttgaat gacggacgga cattgcgcgc ggcgtaaagc tctcgataca gccagctttg
    18361 ttcaaaacgt tgctgataat ccgccgcttc tttgccgctg tcgaaaccct ccgcttcttc
    18421 aaggtgttcc aacaagggga acacggcttc ggcggcgatc atagcggatt tcatcgcggt
    18481 atggatgcct ttgatgcgcg gcatattgag gaaacctgcc gcatcgccga ttaaaacgcc
    18541 gcctttgaac gagagcttcg gcaaactttg caaaccgcct tcaatcagcg aacgcgcgcc
    18601 gtaagcaatg cggcggccgc cttcaaaggt tttgcggatt tcgggatggg ttttgaaacg
    18661 ttggaactct tcaaacggcg acagataagg attttgatag tccaaaccga ccacgaagcc
    18721 gacggcgact ttgttgtcgt cgaaatggta aacaaacgcg ccgccgtagg ttttgctgtc
    18781 cagcggccag cctgcgctgt gcaccaccaa accgggctga tgctgttcgg acggcacttc
    18841 ccaaacttct ttcatgccca agccgtaagt ttgcggctgg ctgttttggt cgagtttgaa
    18901 gcgttcgatg atttgtttgg aaagcgaacc tcggcagccc tcggcaaaca gggtttgccg
    18961 tgcccaaagc tccatgccgg gctggaatga atcggtcggc tcgccgtctt tgcccacgcc
    19021 catattgccg gtcgcaatgc ctttgaccga accgtcttcg tgatacagta cttcggcagc
    19081 ggcaaagcca ggatagattt ccacgcccat attttccgcc tgctccgcca accagcgcac
    19141 gacttcgccc aagctgacga tgtagttgcc gtgattgtcg aaattcgggg taataggcag
    19201 attgaacgcc tttttcttag tcaggaacaa tactttgtcc tgcgttaccg tacgcgtcag
    19261 cggcgcgcct ttttctttcc aatcgggaat cagctcgttc agcgcaatcg gatcgataac
    19321 cgcgcccgcc agcgaatgcg cgcccgtctc agaacctttc tccaccacgc aaacgctgat
    19381 ttcgcgcccg ttttgttcgg caagctgctt gagtttgatg gcggcagaca aacccgacgg
    19441 gcctgcgccg acaatcacga catcgtattg catactgtcg cgggtgatgg attctgtcat
    19501 ggcggttcct gtgtatttat tattgaattg caaatccgta attatacaac gggaacatat
    19561 agttaccaaa tacaacaaag gtcgtctgaa aaccatattt tcggttttca gacgaccttt
    19621 gtcgaaattt caataagcac gccaccattt tacctgtccg accgcaaact ccgtctgacg
    19681 tttcggactg cgtgtgaaaa acgccttatc cccgccggca tccctccctt tcggcac
//
LOCUS       DAWHXZ010000011        19415 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000011 DAWHXZ010000000
VERSION     DAWHXZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.011"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..127)
                     /locus_tag="WIE35_000268"
     CDS             complement(<1..127)
                     /locus_tag="WIE35_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ4153251.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQK"
     gene            complement(190..1008)
                     /gene="hslO"
                     /locus_tag="WIE35_000269"
                     /pseudo
     CDS             complement(190..1008)
                     /gene="hslO"
                     /locus_tag="WIE35_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
     gene            1253..2008
                     /locus_tag="WIE35_000270"
     CDS             1253..2008
                     /locus_tag="WIE35_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ4153252.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            2027..2245
                     /locus_tag="WIE35_000271"
     CDS             2027..2245
                     /locus_tag="WIE35_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153253.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            2390..2734
                     /locus_tag="WIE35_000272"
     CDS             2390..2734
                     /locus_tag="WIE35_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ4153254.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            2734..3426
                     /locus_tag="WIE35_000273"
     CDS             2734..3426
                     /locus_tag="WIE35_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ4153255.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            3493..4713
                     /gene="argJ"
                     /locus_tag="WIE35_000274"
     CDS             3493..4713
                     /gene="argJ"
                     /locus_tag="WIE35_000274"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ4153256.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLSFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            4773..6209
                     /locus_tag="WIE35_000275"
     CDS             4773..6209
                     /locus_tag="WIE35_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ4153257.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLAAGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLTAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTAKRECRLKIKTPPIKRRQPP"
     gene            6364..7755
                     /gene="hrpA"
                     /locus_tag="WIE35_000276"
     CDS             6364..7755
                     /gene="hrpA"
                     /locus_tag="WIE35_000276"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ4153258.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     RRLAALPKPEFDNTLPVHEKLEEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            7856..8983
                     /locus_tag="WIE35_000277"
     CDS             7856..8983
                     /locus_tag="WIE35_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153259.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            9161..10737
                     /gene="lpt3"
                     /locus_tag="WIE35_000278"
                     /pseudo
     CDS             9161..10737
                     /gene="lpt3"
                     /locus_tag="WIE35_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
     gene            10839..13919
                     /locus_tag="WIE35_000279"
     CDS             10839..13919
                     /locus_tag="WIE35_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ4153260.1"
                     /translation="MDARSNPVNVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEIRQLTSSESQGDQDLAAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVPYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYHERLP
                     NFYTADAVSDDLHPTNPQQTAPSPVGEGWGEGKTVAAQTNFSATAANPLPNPLPQERE
                     QSAAASAVSNDLHPANPQQTAPSPVGEGWGEGKTVAAQTNFSATAANPLPQEGEQSAA
                     ASALSDDPQPQKQPASQKGRLKPLPLADIRTFQAWLKTAERENPRLLFLSRDDLMQHA
                     AAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRISPAALEWLVPG
                     MLREKIQLLIKALPKQIRRICVPVPDFITKFLESNPDRQAAIIPQLAHFIAKSAGDMR
                     IFEQIDQDAWAVQELPEHCYLNLRIIDDGGQELAGGRKLHELQQQLGQAAATTFRDNT
                     QEFERDNVTAWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALRLCDTIEAAEQAH
                     RQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGED
                     ELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRL
                     QTLLAPSFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEK
                     TDSLVKQGLPISDGLAGFKWMIEELRVSLFAQELKTPYPVSVKRLMKEWERLNK"
     gene            13978..15585
                     /locus_tag="WIE35_000280"
     CDS             13978..15585
                     /locus_tag="WIE35_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ4153261.1"
                     /translation="MKSENNYERIVQNSKKFQHSQKNEMQKRITDLLQQLTNGIHERD
                     QIMAVSLLGAIAGHNTFLFGPPGTAKSLISRRLACAFENPQYFEYLMNRFSTPEEVFG
                     PVSIKALKEDRYLRKIEGYLPTADFAFLDEIWKSSPAILNNLLTIINEHIFKNGDERF
                     NVPLKALIAASNEIPPENQGLEALYDRFIIRLLVPPIEQEENFNRLLDSKPSSDKPEI
                     SEDLRISYHELAKWREQLHKVRLSTDTLLIIKYIREELAAQFDKLGVYVSDRRWQRAA
                     VLLKASAFCNGRKETNHSDVILLKYCLWTTPENRESVEQIVMNAIKSCGFDTGIDLAE
                     LDREKESLDKEINKELYHSQDVYETVEMSGKKYFPVQAVFKNVHNSHANKELKIYVPY
                     SKFRSKEKFYPIDSTGNEIQEIECEFDGQGCCKLELKVRGGHWNEQYKPLKFKPNILF
                     HKGDKKSDVNERLIHSLTQSISDIRKQLIDTQKQVEKKYEKYQQYLQSPFVAQADIDV
                     AISGILDQIDKLNLRIKDCERLESLCR"
     gene            15576..17036
                     /locus_tag="WIE35_000281"
     CDS             15576..17036
                     /locus_tag="WIE35_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEZ4153262.1"
                     /translation="MSLNRKLSEQIRWIKASLQIDYADLFKQHGSLNGQIQQRLSDWQ
                     YQAKLALLQKNPFILHEEKLKKHQQTEIFNQQSVDDYRYFCKLAGVPFEKEFWQQELT
                     QSKNSKPNKQNLPLKLLTGKWQQQLDYAKSQWYLKQLNQLRQEFIDKLKQELDTIRQL
                     AEQLEQFGFEAGLWFDNSIGNLSQQNIEEMKRWLNYLTQDKNAQQIAELLGKMRQIEQ
                     SEKIEQVKQTVYTKSPQIDVNSREEIIGLRLGKDLEYVLPSELALMADEETSTLFDLK
                     FLESKLMCFELQGMTYCDAPTEIIVEQKSQEDEKLGPMILCVDTSGSMVGLPENIAKA
                     MALFLGAKAKSENRSCFVINFSTGIETFELTSKTGISNLIAFLRQSFHGGTDAAPALR
                     HALKMMEQEIYQKADLLMISDFVMNSLPDDLLASIEIQRETGNQFNSLVIGDAFMSKR
                     LKTHFDREWIYNPNVQTIQELVQFKKDIFNKQVVNS"
     gene            complement(17390..17863)
                     /locus_tag="WIE35_000282"
                     /pseudo
     CDS             complement(17390..17863)
                     /locus_tag="WIE35_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            18095..18307
                     /locus_tag="WIE35_000283"
     CDS             18095..18307
                     /locus_tag="WIE35_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153263.1"
                     /translation="MPHIHPLHLFPPHIAERGLLYFQQGKVGAVQKTSTGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            18604..18759
                     /locus_tag="WIE35_000284"
     CDS             18604..18759
                     /locus_tag="WIE35_000284"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ4153264.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(18849..19298)
                     /locus_tag="WIE35_000285"
     CDS             complement(18849..19298)
                     /locus_tag="WIE35_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ4153265.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         5152 a         5363 c         4503 g         4397 t
ORIGIN      
        1 ttttttgacg gttcaggtat tgttcgatcg gctgccaatc aatcacctgg tccaacttca
       61 atagcgggaa acggtcgatg tgtttggcaa tcatggcttg ggcggtttgt tgaaagaagg
      121 tgctcatgag aaatccccta aatgtcttgg tgggaattta ggggattttg gggaattttg
      181 caaaggtctc ttgctgtggc agaaatcgca atcgacttcg atgctgcctt gttccaccac
      241 cacgccgccg acttcttccc cgcccagcat cagcagcata tcgctgactt tgccgcgcga
      301 acaggtgcat gaaaattcaa acgtttccgg ctcgaacacg cgcggcggcg tttcgtggaa
      361 caggcggtac aaaacatgtt gcgcatccaa ttctgccagc tcgtccgccg tcagtgtacg
      421 cgccagcgta ctgacgtgtt cccatgcctc ttcatccaac accgcttcag gcagacgctg
      481 caccagcaga ccgcccgccg cttcgtcgct tgcagacagg acgatgtgcg tatcgatttg
      541 ttcggaacgc ttcatatagt tcgccaacat ttgcgcgata ccgccgcctt ccaaaggcac
      601 tacgccctgc cagggttcgc cgtctttggg ctgcagcgtc agcacgaata cgccgccctc
      661 gcccaaaagg tcgccgaggc tttcgtcatc ggctatttct gcggtttcgt cccaacgcgc
      721 ggttgcacgg acggtacggt cggaagccgc ttccgcaacc agcattttca gccgcccccg
      781 cccctgaacc tgcacaatca gcgtgccttc gtttttgagg ttgcccgaca gcaacacacc
      841 cgccgccaac aactcaccca aagcgcggcg gatggcggcg ggatagtttt tctgttttac
      901 aatgtgctgc cacacgtttt ccagacggac gtgcagcccg cgcacgggca tatcgtcgaa
      961 gataaagcgg gtacgcacat cggcgcggtt gatggcggtt tgattcatga ttttctctga
     1021 ctgattgttc ggatggcggc tatatggttg cggtcggcgc gaaaacaaga cggacggcgg
     1081 atgcgcttcc caaattatca ataaattata taaaaatcaa catattaact caatctaaca
     1141 agccgttttt tgccaaacag ccgttttttt atatacaatc aacaagatat tttcgactga
     1201 tacagcataa catcgcacgg cggcacgatg cctcctgcgc ggaaacaccg atatggattc
     1261 ttttttcaaa ccggcagttt gggcggtttt gtggctgatg tttgccgtcc gccccgccct
     1321 tgccgacgag ttgaccaacc tgctcagcag ccgcgagcag attctcagac agtttgccga
     1381 agacgaacag cccgttttac ccatcaaccg agcccccgcc cggcgggcgg gcaatgccga
     1441 cgaactcatc ggcagcgcga tggggcttaa cgaacagccc gttttacccg tcaaccgagt
     1501 ccccgcccgg cgggcgggca atgccgacga actcatcggc aacgcgatgg ggcttaacga
     1561 acagcccgtt ttacccgtca accgagcccc cgcccggcgg gcgggcaatg ccgacgaact
     1621 catcggcaac gcgatgggac ttttgggtat tgcctaccgc tacggcggca catcggtttc
     1681 taccggtttt gactgcagcg gcttcatgca gcacatcttc aaacgcgcca tgggcatcaa
     1741 cctgccgcgc acgtcggcag aacaggcacg gatgggtacg ccggttgccc gaagcgaatt
     1801 gcagcccgga gatatggtgt ttttccgcac gctcggcggc agccgcattt cccatgtcgg
     1861 actttatatc ggcaacaacc gcttcatcca cgcgccgcgc acggggaaaa atatcgaaat
     1921 caccagcctg agccacaaat attggagcgg caaatacgcg ttcgcccgcc gggtcaagaa
     1981 aaacgacccg tcccgctttc tgaactgatt tcccaaggaa tacgcaatga gtatgcccga
     2041 aatgcccaaa tggtacgacg atgacggaca gatcgtgtcc tgtaccgaaa aggtcaaagt
     2101 gatgtccgaa aatatggccg agctgtatca gacggcacaa gacgcgtttg aagacgcgct
     2161 gctgatgggt tgcggcgaac gtcaattgcg cgattacctg ctcgcgctga ttgaaggttt
     2221 ggaaaatccc taccgcaaag tctgaacacg ccccggttgc tgcggcacgg tttatccgtg
     2281 ccgtttttgc gtttgtgcgc ggcttcggct tttcagacgg catatttgac gttatgatta
     2341 aacagttaac aagatttatc acaacgccgt caaaagacag acacacaaca tgaacatcat
     2401 ccgcgcgctc ctcatcatcc tcggctgcct cgccgccggc gaaaccgccg ttttcctagc
     2461 aggcatcaaa ctgcccggca gcatcgtcgg catgggcgtg ctgtttgcgc ttttgcaggc
     2521 gggttgggtc aaaacgtctt ggctacaaca gcttaccgac gcgctgatgg cgaatctgac
     2581 gctgtttctc gtgccgccct gcgtggcggt catcagctat ttggatttga ttgccgacga
     2641 ttggttttcg atactggttt ccgcctccgc cagcactttg tgcgtactgc tggttacagg
     2701 caaggttcac cgctggatac ggagcattat ctgatgaacg aaatcctcag gcagcccagc
     2761 gttctgcttt tcctcacgct tgccgtgtac gcgcttgcga ttatcgtgcg cacgcgcacg
     2821 ggcaatatct tctgcaaccc cgtactcgtc agcactatcg tgctgattgc ctacctgaaa
     2881 atcctcgaca tcgattatgc ggtgtaccac aacgccgcgc aattcattga tttttggctg
     2941 aaacccgccg tcgtcgtgct tgccgtgccg ctctaccaaa accgccgtaa aatcttcaac
     3001 cagtggctgc ccgtcatcgt ttcacagctt gcgggcagcg ttacgggcat tgttacgggg
     3061 atgtattttg ccaaatggct gggcgcggaa cgcgaagtcg tcctctcgct cgcgtccaaa
     3121 tctgttacca accccatcgc cattgaaatc acccgctcca tcggcggcat tcccgccatt
     3181 accgccgcca ccgtcatcat tgccggtctg gtcggacaga ttgccggtta caaaatgctg
     3241 aagaacacag tcgtcatgcc ctcgtccgtg ggtatgtcgc tcggcacggc ttcgcacgcg
     3301 atggggattg ccgcctcgct cgaacgcagc cgccgtatgg cggcatacgc gggactgggg
     3361 ctgacgttca acggcgtact gaccgcgctg attgcgccgc tgctcatccc cgttttggga
     3421 ttttgaaccc gtttcagacg gcatttcagc ccatgctgtc tgaacgccga cacactcgca
     3481 aggagaaccg ttatggctgt caacctgacc gaaaaaaccg ccgaacaact gcccgacatc
     3541 gacggcattg ccctctacac cgcccaagca ggcgtgaaga agcccgggca taccgacctg
     3601 acactgattg ccgtagccgc cggcagcacc gtcggtgcag tcttcacgac caaccgtttc
     3661 tgtgccgcgc ccgtccacat cgccaaatcg caccttttcg acgaagacgg cgtgcgcgcc
     3721 ctcatcatca acacgggcaa cgccaacgcg ggtacgggcg cacagggcag aatcgatgct
     3781 ttggcagtgt gtgccgccgc cgcccggcaa atcggctgca aaccgaacca ggtgctgccc
     3841 ttctccaccg gcgtgattct cgaaccgctg cccgcagaca aaatcatcgc cgcgctgccc
     3901 aaaatgcagc ctgccttctg gaacgaagcg gcacgcgcca tcatgaccac cgacaccgtg
     3961 cccaaagccg cctcgcgcga aggcaaggtc ggcgacaaac acaccgtccg cgccacgggc
     4021 atcgccaaag gctcgggcat gattcatccc aatatggcga ccatgctcag tttcatcgcc
     4081 accgatgcca aagtttccca acccgtcctc caactgatga cgcaggaaat cgccgacgaa
     4141 accttcaaca ccatcaccgt tgacggcgac accagcacca acgacagctt cgtcatcatc
     4201 gccaccggca aaaacagcca aagcgaaatc gacaacatcg ccgacccgcg ttacgcccaa
     4261 ctcaaagaat tgttgtgcag cctcgcgctc gaactcgccc aagccatcgt ccgcgacggc
     4321 gaaggtgcga ccaagttcat caccgtccgc gtcgaaaacg ccaaaacccg cgacgaagcc
     4381 cgccaagccg cctacgccgt ggcacgttcg ccgctggtca aaaccgcctt tttcgcctcc
     4441 gaccccaacc tcggcagact gctcgccgcc atcggttatg ccggcgttgc cgacctcgat
     4501 accgacctcg tggaaatgta tctcgacgat attttggttg ccgaacacgg cggacgcgcc
     4561 gcaagctaca ccgaagcaca agggcaggcg gtgatgtcga aggccgaaat caccgtccgc
     4621 atcaagctgc atcgcggaca agccgccgcc accgtctata cctgcgacct gtcgcacgga
     4681 tacgtttcca tcaacgccga ctaccgttcc tgacccgaca cggcttcaga cggcatacat
     4741 aaaatgccgt ctgaaccgcc ggacaacata ccatgacctc cacattcccc cgccgcctcg
     4801 cccgcaaaat ccgccaaacc cgccgcctgt cgcgcaaaag catcgccttt ctgttccttt
     4861 tggcaggttc ggcactcgtc gccctgaccg cgctgttttt tgcccatctt gccgattttg
     4921 cgctggaact gaacgccaaa ctggttcaac aatacccgtg gttcgcgtgg gtcgcgcttc
     4981 ctttgggttt accgcttatt gcgtggctca cacgcaaatt cgcccccttc accgccggca
     5041 gcggcatccc gcaggtcatc gcctcactgt cgctgcccta cggcgcacag aaaacgcggc
     5101 tgatccgcct cgggcagaca ctgctgaaga ttccgctgac ctttttgggt atgctgttcg
     5161 gcgcgtccat cggacgcgaa ggtccgtccg tgcaggtcgg cgcggcagtg atgggcgcgt
     5221 ggggcgcgtg gtgcaagaaa cacggcttgg cattcaaagg gatgcaggaa aacgatttga
     5281 tggcggcggg cgcggcgggc ggtttggcag ccgcgttcaa cgcgccgctg gcgggcgtga
     5341 ttttcgccat tgaggaactc gggcgcggca tcatgttgcg ctgggaacgg cagattctgc
     5401 tcggcgtact cgccgccggt ttcatacagg tcgccattca gggcaacaac ccgtattttt
     5461 ccggcttcaa cggcggcgta ttggaacata tctttctgtg ggtcgcactg tccggcctgg
     5521 tttgcggcgc ggcgggcggg ctgttcggac gtttgctcta tcgcggtgcg gcggcgtttg
     5581 caccgcgcaa gatacgcggc ttcatccgca accgtccgct gctgctggcg gcactgatgg
     5641 ggctgctgct cgccctgctc ggcacgttct accaaggcaa aacctacggc accggctacc
     5701 acgaagccgc ccaagccctg cacggcatct acgaagcccc cttcggactc accgccgcca
     5761 aatggctcgc caccgtattc agctattggg caggcattcc gggcggcatt ttcactccct
     5821 cgctgaccat aggcgcggtt ttgggcgagc atatcgccgc catcgccgac atatcgcagg
     5881 gtgcaaacat catcgtcctc atctgcatgg cggcatttct ggcgggcgcg acacaatccc
     5941 cgattacttc cgccgtcgtc gtcatggaaa tgacgggcgg acaaagcctg ctgttttgga
     6001 tgctgattgc ctgcattttc gcctcgcagg tttcgcgcca gttttcgccg cgtccgttct
     6061 accacgcatc gggaatgcgc ttccgccagc gcgtgcttca agaaaccgcc gcccaaaccg
     6121 gcaatgcgcc cgcaagaccg caaacagcaa aacgggaatg ccgtctgaaa attaaaacgc
     6181 ccccgatcaa acgccggcag ccgccttgat tcgaataccg ttccgccgcc gcttgaaatt
     6241 tcagcaacaa tgccgtctga acgacagaat gcggttttca gacggcattt ccccatcccg
     6301 atattgccta aacaaaaccg aagcgtttgc tataattgca ctttttaccg cacacgcacg
     6361 cccatgtttc ccgatttctc ccaaaccctc tccaaagacc gccacttcct gcaatccgcc
     6421 ttcaaaaatc ccaacaaata cggcggtttg tccaaagtcg aagaaaaata ccgaaaatcg
     6481 cacgaaatct ttttgaggcg tttggccgca ttgcccaaac ccgaattcga caacaccctg
     6541 cccgttcacg aaaaactcga agaaatcaaa aaaaccgttg ccgaacatca ggtaacgatt
     6601 atttgcggcg aaaccggttc gggcaagacc actcagttgc ccaagatttg tttggaactc
     6661 gggcgtgggg cggcggggct gatcgggcat acccagccgc gccgtttggc cgcgcgctcc
     6721 gtggcagagc ggattgccga agagctgaaa tccgaaatcg gcagcgcggt cggctataaa
     6781 gtacgcttca ccgaccacac ctcgcgcgat gcctgcgtca agctgatgac cgacggcatc
     6841 ctgctggcgg aaacgcagac cgaccgttat ctcgccgcct acgacacgat tatcatcgac
     6901 gaagcgcacg agcgcagcct gaacatcgac ttccttttgg gctatttgaa acaactcctg
     6961 ccgcgccgcc ccgatttgaa agtcatcatc acctcggcaa cgatagacgc agaacgcttc
     7021 tcccgacact tcaacggcgc gcccgtttta gaagtgagcg gacggacgta tcccgtcgaa
     7081 atcctctacc gaccgctgac cggcaaagac gaagacgacg cagaagtgga gttgaccgac
     7141 gcgattgtcg atgcggcgga cgaattagcg cgacacggcg aaggcgatat tttggtattc
     7201 ctgccgggcg agcgcgaaat ccgcgaagcc gccgaagccc tgcgcaaatc cacgctgcgc
     7261 cgcaacgacg aaatcctgcc cctgttcgca cgcctgtcgc acgccgagca gcacaaaatc
     7321 ttccacccct caggcgcgaa acgccgcatc gtattggcaa ccaacgtcgc cgaaacctcg
     7381 cttaccgtgc cgggcatcaa atacgtcatc gacaccggcc tcgcgcgtgt taaacgctat
     7441 tccgcacggg cgaaagtgga gcagcttcat atcgaaaaaa tctcccaagc cgccgcccgc
     7501 caacgatccg gccgctgcgg acgcgtctcc gcaggcgtgt gtatccgact gttttcagaa
     7561 gaagatttca acagtcgccc cgaatttacc gaccccgaaa tcgtccgcag caacctcgcc
     7621 gccgtcatcc tgcgcatggc atcgctgaaa ctcggcgacg tagcggcatt cccattttta
     7681 gaaatgcccg attcgcggta tattaatgat gggtttcagg tgttgctgga attaggggcg
     7741 gtggaggccg cctgaaaaca agtaggatat acacttctac ctgacaaaga ccaacaaacg
     7801 ataaaactac ggcacatcta gatgcccaac ctacaacatt gcacacggta aaaacatgaa
     7861 acacttactc atcgattttg aaaacgtcca gccgcaaaac ttagacaaat tactaaccga
     7921 aaatacccat atttggctat ttataggtgt attacacaaa atgttaccta ttagtctggt
     7981 gcaatcccta ctacgtttcg gcgaacgtgt ccatcttgtc cagttacaaa aaacagggaa
     8041 aaacgcattg gatttttacc tgtcctatta cctcggacaa attaccgcca cagaccccaa
     8101 tgcccaaatc ggcatactct cgcgtgatgg aggatacgat gttctggtcg aacatatttt
     8161 gaaaaaccac caggcgaagg gtatcgtgcg cctagccaat atagatgaag tacaacatca
     8221 gaaaattact accgaaccgc cgtcagcatt gctggaaaac actcctcagc ctgaaaccac
     8281 cctcaaacca cagcaaccat taacttccta tttccaagca gccctaactg cactgcgccg
     8341 ccccgacgct ttccgcccct gccgcctgca taacctgcga caaaatctgc gtaagcatat
     8401 tttgagtgat ttgtttaaag aaaaaaccga tgaagaatgc gaaataacca ctgctaacgt
     8461 tatcaataaa ctcaaagcac aaaacttcat cagcattgat gaacaggaaa ccgtttccta
     8521 ccatctcagt gataatgatt tgttacaaag aatccaacgc catattttaa gccaacgtcc
     8581 caaaacctac gctgattttc aagccgtcgt gcaaaaccga gcagatgcac ttcacttaac
     8641 agtcggtacc aacgacattc aatcctttgc gcgacatttg cgcgaccaaa acctgatccg
     8701 ccaaaacaat gggaaaattg aatatgcacc gtttactgaa cctaaaccac agccaacgcc
     8761 caagcagcct aaaaaaaccg catgggaacc tgatgaaatt atttggaaaa aagtgattgc
     8821 cgcgttatcg ttaaagaacc gtcctaataa aaccaaaact ttacgcaata caatccaggc
     8881 actcacaaaa tccaatgcac aagaaactga caaactgcta caacatttac aagataccca
     8941 agtcctacgt attgacggta ctaaaattgt gtatatcaag taggatgggc atttatgcct
     9001 gacctacaaa accaaaaaca acctaccacc cttaatcaac tccacaaacc cttttcagac
     9061 aacctcgttt tttgaaaaac aatctgtaaa cagatagctg ctgaagaata ccgttgccga
     9121 gccccaaaac ccgtcctgca acttttattg tgaacttccc attatgaaaa aatccctttt
     9181 cgttctcttt ctgtattcgt ccctacttac tgccagcgaa attgcttatc gctttgtatt
     9241 cggaattgaa accttaccgg ctgcaaaaat ggcggaaacg tttgcgctga catttgtgat
     9301 tgctgcgctg tatctgtttg cgcgttataa ggcgacgcgt ttgttgattg cggtgttttt
     9361 tgcgttcagc attattgcca acaatgtgca ttacgcggtt tatcaaagct ggataacggg
     9421 cattaattat tggctgatgc tgaagagatt accgaagtcg gcggcgcggg cgcgtcgatg
     9481 ttggatcagt tgtggctgcc tgcgttgtgg ggcgtgttgg aagtcatgtt gttttgcagc
     9541 cttgccaagt tccaccgtaa gacgcatttt tctgccgata tactgtttgc cttcctaatg
     9601 ctgatgattt tcgtgcgttc gttcgacacg aaacaagagc acggtatttc gcccaaaccg
     9661 acatacagcc gcatcaaagc caattatttc agcttcggtt attttgtcgg acgcgtgttg
     9721 ccgtatcagt tgtttgattt aagcaggatt cccgccttta agcagcctgc tccaagcaaa
     9781 atcaggcagg gcagtgttca aaatatcgtc ctgattatgg gcgaaagcga aagcgcggcg
     9841 catttgaagc tgtttggcta cggacgcgaa acttcgccgt ttttaacccg gctgtcgcaa
     9901 gccgatttta agccgattgt gaaacaaagt tattccgcag gctttatgac tgcagtgtcc
     9961 ctgcccagtt ttttcaatgc gataccgcac gccaacggct tggaacaaat cagcggcggc
    10021 gataccaata tgttccgcct cgccaaagag cagggctatg agacgtattt ttacagtgct
    10081 caggctgaaa accaaatggc aattttgaac ttaatcggta agaaatggat agaccatctg
    10141 attcaaccga cgcagcttgg ctacggcaac ggcgacaata tgcccgatga gaagctgctg
    10201 ccgttgttcg acaaaatcaa tttgcagcag ggcaagcatt ttatcgtgtt gcaccaacgt
    10261 ggttcgcacg ccccatacgg cgcattgttg cagcctcaag ataaagtatt cggcgaagcc
    10321 gatattgtgg ataagtacga caacaccatc cacaaaaccg accaaatgat tcaaaccgta
    10381 ttcgagcagc tgcaaaagca gcctgacggc aactggctgt ttgcctatac ctccgatcat
    10441 ggccagtatg ttcgccaaga tatctacaat caaggcacgg tgcagcccga cagctatctc
    10501 gtgccgctag tgttgtacag cccggataag gccgtgcaac aggctgccaa ccaggctttt
    10561 gcgccttgcg agattgcctt ccatcagcag ctttcaacgt tcctgattca cacgttgggc
    10621 tacgatatgc cggtttcagg ttgtcgcgaa ggctcggtaa cgggcaacct gattacgggt
    10681 gatgcaggca gcttgaacat tcgcgacggc aaggcggaat atgtttatcc gcaatgagtg
    10741 gcgtaaaaac caataaagac aaatttagat gatgtcgggg aagatgcccg accgacaaga
    10801 ctatgcaaaa tatgaaaaac caagtacgcg gatcaggcat ggatgcccga tccaatccgg
    10861 tcaatgtttc agacggcctg caaaacagtt cgggtcatat cggtaccaac acgcgttacc
    10921 gcctgaccaa actcggcgaa caaatggcgc gcctgcccat cgacccgaaa atcgcgcgca
    10981 ttttgctggc ggcgaagaaa cacgactgca tggcggaaat attggtgata gcgtccgcgc
    11041 tgtcgattca agacccgcgc gagcggccgt tggaagcgcg cgatgccgcc gccaaggcac
    11101 acgagcgttt taccgacaag caatccgact tccttgccta tctgaacatt tgggacagct
    11161 tccagcgcga gcgcgacaaa ggtttgtcca acaagcagtt ggtgcaatgg tgccgtcaat
    11221 atttcctgtc gcacctgagg atgcgcgagt ggcgcgagtt gcaccaccag cttgcccaaa
    11281 ccgcgattga aatgggtttg accaccaagg aagcggcttt cagacggcct cccgaaatca
    11341 ggcagctcac gtcgtctgaa agccaaggcg accaagattt ggcggccaaa ctcaaacaaa
    11401 aacaactgga taagaagcaa caccgcgccc aaatccgcgc cgccaaagaa gcgggctacg
    11461 aacaaatcca ccgcgccctg ctgaccggcc ttatcgccaa cgtcggcatg aaatcgcccg
    11521 acggcaacga ctacaccggc gcacgcggca gccgttttca tcttttcccg gcatcagccc
    11581 tgttcaaagc caagcccaaa tgggtgatgg cggcagaatt ggttgaaacc acgcgccttt
    11641 acgcgcgcga cgtcgccgtt atccagcccg aatggataga gcaggaagcg ccgcacctcg
    11701 tccgctatca ttatttcgag ccgcattggg aacaaaaacg cggcgaagtc gtcgccagcg
    11761 aacgcgtgac gctttacggt ctgaccgtat tgccgcgccg ccccgtgcct tacggcaaag
    11821 ttgcgcccga agaagcacgc gaaatcttta tccgcagcgc attggtggcg caggaatgcg
    11881 atttgaaagc ggattttttt gtccacaaca aaaagctgat taaagaaatt accgaactcg
    11941 aacacaaatc gcgcaagcaa gacgtattgg tcgacgacga agccctgttt gcgttttatc
    12001 acgaacggct gcccaatttt tatacggcgg acgcggtttc agacgacctg catcctacaa
    12061 atccgcagca aactgccccc tcccccgtgg gggagggctg gggagagggc aaaacagttg
    12121 ccgcacaaac caacttttcc gcaaccgcag caaaccctct ccctaaccct ctcccacagg
    12181 agagggagca gagtgccgca gcttcagcgg tttcaaacga cctgcatcct gcaaatcccc
    12241 aacaaaccgc cccctccccc gtgggggagg gctggggaga gggcaaaaca gttgccgcac
    12301 aaaccaactt ctccgcaacc gcagcaaacc ctctcccaca ggagggggaa cagagtgccg
    12361 cagcttcagc actttcagac gaccctcaac cccaaaagca gccagcatct caaaaaggcc
    12421 gtctgaaacc cctccccctc gccgacatcc gcaccttcca agcctggctc aaaaccgccg
    12481 aacgcgagaa cccgcgcctg ctgttcctca gccgcgacga tttgatgcaa cacgccgccg
    12541 cacacattac cgaagaacaa ttccccaaat tctggcaaac cgcagacggc aaattcaaac
    12601 tcagctaccg cttcgagccg caccacccgc tcgacggcgt taccctcacc ctgccgctca
    12661 ccgtgctcaa ccgcatcagc cccgccgccc tcgaatggct cgtgcccggt atgttgcgcg
    12721 agaaaatcca gctgctgatt aaagcactgc cgaagcaaat ccgccgcatt tgcgtgcccg
    12781 tacccgattt cattaccaaa tttctcgaaa gcaatcccga ccgccaagcg gccatcattc
    12841 cccagttggc gcactttatc gccaaaagcg caggcgatat gcggattttc gagcaaatcg
    12901 accaagacgc atgggcggtg caagaattgc ccgaacactg ctatctgaat ctgcgcatta
    12961 tcgacgacgg cggacaagag cttgccggcg gccgcaaact gcacgaattg caacaacagc
    13021 tcggccaagc cgccgccacc accttccgcg acaacaccca agaattcgag cgcgacaacg
    13081 ttaccgcatg ggacatcggc accctacccg aatccatcaa attcgcacgc ggcaaacaac
    13141 agctcaccgg ctatctcggt ctgcaaaaag aaaaagacgg ccgcatcgcc ctgcgcctgt
    13201 gtgacaccat cgaagccgcc gagcaggcac accgtcaagg cgtcatcgaa ttgatgaagc
    13261 tgcaattaaa agagcaggta aaagacctga acaaaggcat ccaaggcttc acccaagccg
    13321 ccatgctgct caaacacatc aacgccgaca ccctgcgcga cgacctcacc caagccgtct
    13381 gcgaccgcgc ctttatcggc gaagacgagc tgccgcgcaa cgaaaaagcc ttcaaagaac
    13441 aaatcaaacg cgcccgcagc cgcctgcccg ccgtcaaaga agctctcagt cgttacctgc
    13501 aggaaaccgc cgccgcctat gccgaactga acggcaaact cggcaaacac ccgctcaccc
    13561 atctcctaag actacgcctg caaaccctgc ttgcccccag cttcgccacc cgcaccccgt
    13621 gggcacaatg gccgcgcctc cccatctatc tcaaagccat gaccctgcgc ctcgaaaaat
    13681 acagcagcaa ccccgcccgc gacgcagccc gcgaagccga tatccaagag ctggaacaaa
    13741 tgtggcagga aaaaaccgat agcttggtga aacaaggatt gcccatttca gacggccttg
    13801 cagggtttaa atggatgatt gaagaattga gggtgtcgct gtttgcgcag gaattgaaga
    13861 caccgtatcc ggtgtcggtg aagcggctga tgaaggagtg ggagaggttg aataagtagt
    13921 gatattatcc aatacattta caggaaaatt tctaacttac ataaaaggta caactcaatg
    13981 aaaagcgaaa ataattatga acgtattgtt caaaattcta aaaaattcca acactctcag
    14041 aagaatgaga tgcagaaacg cataacagat ttgctgcaac aactcacaaa tggcattcat
    14101 gaacgcgacc aaattatggc ggtttcccta cttggtgcga ttgccggaca taatactttc
    14161 ctatttggac cgccgggaac cgcgaaaagt ctgatttccc gccgtttggc atgtgcattt
    14221 gaaaatccgc aatactttga atacttgatg aaccgtttca gcacacctga agaagtattc
    14281 ggacctgtat ctattaaggc attgaaagaa gaccgatatc tccgaaaaat cgaaggctat
    14341 ttgccaactg ccgattttgc atttttggat gaaatttgga aatccagtcc tgcaattttg
    14401 aataacctgc tgaccattat caacgagcat atttttaaaa acggcgatga gaggtttaat
    14461 gtgccactta aagccctgat tgcggcttcc aacgaaattc cacctgaaaa tcaaggttta
    14521 gaggcactgt atgaccgctt tattatccgt ctgcttgtgc cgccgattga acaagaagag
    14581 aacttcaacc gtttgctgga tagcaaacca agcagcgaca aacctgaaat cagtgaagat
    14641 ttacggatta gttatcatga gcttgccaaa tggcgtgaac aactccacaa agtcaggcta
    14701 agtacagata cattactgat tattaagtat atcagagaag aattagcagc ccagtttgat
    14761 aagttaggcg tttatgtatc cgaccgacgt tggcaacgtg cagccgtatt attaaaggct
    14821 tctgcttttt gcaatggacg caaagaaacc aatcatagcg atgtgatctt gctgaaatat
    14881 tgtctgtgga caacacctga aaatcgagag tcagtggaac aaattgtgat gaatgccatt
    14941 aaatcctgtg ggtttgatac aggtattgat ctggctgaat tagatagaga aaaagagagt
    15001 ttggataagg aaataaataa ggaactttat cattcacaag atgtttatga gacggttgaa
    15061 atgagtggaa aaaaatattt tccagtacag gctgttttca aaaacgtaca taatagtcat
    15121 gctaataaag aacttaagat ttatgttcca tatagcaaat ttcgtagtaa agaaaaattt
    15181 tatccaatag atagtacagg aaatgaaatt caagaaattg aatgtgaatt tgatgggcaa
    15241 ggttgctgta aattggaatt gaaagtgcgg ggtggtcact ggaatgagca atataagcca
    15301 ttaaaattta aacccaatat cttatttcac aaaggagata aaaaatcaga tgttaacgaa
    15361 cgtctaattc attcactaac ccaatctata tctgatattc gcaaacaatt gattgataca
    15421 cagaaacaag ttgagaaaaa atacgaaaaa taccagcagt acctgcaaag cccctttgtt
    15481 gcccaagcag atattgatgt tgctatatca ggtattctag accaaatcga taaactcaat
    15541 ttgcggatta aagactgcga acgtttggag tctttatgtc gttaaacaga aaattatccg
    15601 aacaaataag atggattaaa gcttctctac aaatagatta tgcagattta tttaaacagc
    15661 atggttcgtt aaatggacag attcagcaac gtttatcaga ttggcaatat caggccaaac
    15721 tggctttgtt gcagaaaaac ccatttatat tacatgaaga aaagctaaaa aaacaccaac
    15781 aaacagaaat ttttaatcaa cagtctgttg atgattatcg gtatttttgc aaattagcag
    15841 gcgtgccatt tgaaaaagaa ttctggcagc aggaattaac acaaagcaaa aattcaaaac
    15901 ccaataaaca gaatttgcct ttgaaattgc taacaggaaa atggcagcag caattagact
    15961 atgccaaatc tcaatggtat ttaaagcaac ttaatcaatt acgacaggaa tttatagata
    16021 agttaaaaca ggaactcgac actatccgac agcttgcaga acagttagag caatttggtt
    16081 ttgaagcagg gctgtggttt gataacagta tcggcaattt aagccagcaa aatattgaag
    16141 aaatgaaacg ctggctgaat tacttgaccc aagataagaa tgcccaacag attgctgaat
    16201 tactaggcaa aatgcgccaa atcgaacaat ctgaaaaaat cgaacaggta aaacagactg
    16261 tttacacaaa aagcccacaa attgatgtga attcgcgtga agaaattatc ggattacgtt
    16321 taggtaaaga tttagagtat gtattgccgt ctgaattggc tttaatggca gatgaagaaa
    16381 catcgacttt atttgatttg aagtttttgg aatccaaatt aatgtgtttt gaattgcaag
    16441 gaatgaccta ttgcgatgcc ccaactgaaa ttatagttga acaaaaaagc caagaggatg
    16501 aaaagctcgg accaatgatt ttatgtgtag ataccagtgg ttcaatggtt ggtctacctg
    16561 aaaatattgc caaagcaatg gctttatttt taggagcaaa agctaaaagc gaaaaccgtt
    16621 cttgttttgt tatcaatttt tccactggaa tagaaacttt tgaattaaca agtaaaacag
    16681 ggatttccaa tttgattgct tttttacgtc aatcctttca tggtggtaca gatgctgccc
    16741 cagctttacg tcatgcgcta aaaatgatgg agcaagaaat ctatcaaaag gcagacttat
    16801 taatgatttc cgattttgtg atgaacagct tacctgatga tttgttggca tcaatcgaaa
    16861 tacaacgaga aaccggtaat cagtttaatt ctttggttat tggtgatgct tttatgagta
    16921 aacgcctaaa aacacatttt gatcgtgagt ggatatacaa tcctaatgtt cagacaattc
    16981 aagaattagt acagttcaag aaagatattt tcaataaaca agttgtcaac tcataacact
    17041 atcctactca tatgtatttt tggaagatac caccctatac aacttggaat ccatacgcag
    17101 aatatgtgtg gactttctaa actactcccc tttccaccct aaaaaaggtc gtctgaaacc
    17161 cattttcaga cgaccttttt ccacttcgca aaacaagaat tcccacccct ctccccaaac
    17221 gtcgcccgaa ccagtaaact ccccatattt caacaacttc ttggaagcca accatgcccc
    17281 gtatccaccc tcctcacctg ttcccgcccc atatcgccga acgcggcctt ttgtattttc
    17341 agcagggcaa ggttcgcgat gtccgaaaaa cttccgccgg gcgttatcgg gcctggcgcc
    17401 gtttgctgcc ggcggtctga ataatggtgg cgtcaacgac ggcagcggat gctttctcta
    17461 cttttaggtt tttttcggtc agttggcggt taatcagttt gagcaattcg gacagggtgt
    17521 cgtcttgcgc cagccagttg cggtaacggc ataaggtgct gtaatcgggg atgctcagtt
    17581 cgtcaaagcg gcaaaacagg ttgaagtcga tgcgggtgat gaggctgtgc tcgagttcgg
    17641 gatcggagag gctgtgccat tgtccgagca gaacggcttt gaacatggac aacagcgggt
    17701 aggcgggacg accgcggtgg tctcggaggt aacgggtttt ttgacggttc aggtattgtt
    17761 cgatcggttg ccaatcaatc acctggtcca acttcaatag tgggaaacgg tcgatgtgtt
    17821 tggcaatcat ggcttgggcg gtttgctgaa agaaggtgct catgagaaat ctcctaaatg
    17881 tcttggtggg aatttagggg attttgggga gttttgcaaa ggtctcagtc tttttaaacc
    17941 acagcccttc ccaactaaac caaaaggtcg tctgaaccct attttcagac gaccttttgc
    18001 cactttgtaa aacaaatctt cccaccatcc tctccccaaa catcgcccga accagtaaac
    18061 ttctcatatt tcaacaactt cttggaagca aaccatgccc catatccacc cgctccacct
    18121 gttcccgccc catatcgccg aacgcggact gctctatttt cagcagggca aagttggtgc
    18181 tgtccaaaaa acttccaccg ggcgttatcg ggcggaggta tgcggtttgg aaaactattg
    18241 ggtatggctg aagctggata gtgatttgta tattaaagac gaaagctgcg attgccctta
    18301 tgtctaaagg tgcaaacata ccttaaatta ctatattaca taggcaaaat acaagcctat
    18361 aacgaattgg aaacaaaatg ccgtctgaaa gcattataaa atctgttcac cttttcagat
    18421 gagtaatgta cacccctata caatttttgc tactttaccc cataaatcca cggctaaaga
    18481 tatccttatt atgtcctatg atttatcgaa acgacttgta atcggcttag catcaagtgc
    18541 cctattcgac ttatccgaat cggataatat atttagaatg gaaggggcag aaacctatag
    18601 gcaatatcag agagaaaaac aaaaccatcc cctaaaaaag gcgttgtctt tccatttatt
    18661 aaaaaacttc tgtcaatcaa tgaaataaac ccaaacgacc caacgattgg gtttattctt
    18721 ttatccagaa acaatccaga tacagattac gagtcataac tataggctta atattacacg
    18781 attctcattc catcaaggcg gaaaaccgca caaatactga aacactatcg atcgatttgt
    18841 aaacaagcct acttaagtaa cttgcagtcc ttatcatttc ctttaaaata atccagcccg
    18901 tcactacacg aactggcgga cttcttgcaa ataaaggtta ctagattttc attcatctta
    18961 ataataaaag gatttttatc tttatctatg gctaccgcct tcaacatgaa tttactgtct
    19021 aaggccccgc gcgcgattcc attcaaacgg atacaaaagc cttctgcctc tttaatcggc
    19081 aaacttggcc atttggtaga tgtttgttta aatctcccat tctgcagata aaacttttcc
    19141 ataaaatgtg cattttctaa caaggctgcc cgcactgcat ttatctttgc tttctcaaca
    19201 taattgcgat agctcggata aacaattaaa gcaagtacag acaatatcaa gaccactgat
    19261 attaattcaa ccagcgtaaa cccccgatta tcagtcatta ctttacttcc aataagaaca
    19321 gattattcaa catatttctt tgaacagact tactatccca ttcagcagta tgcatatttc
    19381 ccactctatt ttttatccgc gggcagcact ggttt
//
LOCUS       DAWHXZ010000012        18807 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000012 DAWHXZ010000000
VERSION     DAWHXZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18807
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.012"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(79..770)
                     /locus_tag="WIE35_000286"
                     /pseudo
     CDS             complement(79..770)
                     /locus_tag="WIE35_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1076..1633)
                     /locus_tag="WIE35_000287"
     CDS             complement(1076..1633)
                     /locus_tag="WIE35_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153266.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(1877..2035)
                     /locus_tag="WIE35_000288"
     CDS             complement(1877..2035)
                     /locus_tag="WIE35_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153267.1"
                     /translation="MKTPLPICTLSALAACALSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2032..2874)
                     /locus_tag="WIE35_000289"
     CDS             complement(2032..2874)
                     /locus_tag="WIE35_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ4153268.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKTQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3112..4032
                     /gene="pyrB"
                     /locus_tag="WIE35_000290"
     CDS             3112..4032
                     /gene="pyrB"
                     /locus_tag="WIE35_000290"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ4153269.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLDASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4042..4500
                     /locus_tag="WIE35_000291"
     CDS             4042..4500
                     /locus_tag="WIE35_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ4153270.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4565..5230
                     /locus_tag="WIE35_000292"
     CDS             4565..5230
                     /locus_tag="WIE35_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153271.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALDLGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5293..6510)
                     /locus_tag="WIE35_000293"
     CDS             complement(5293..6510)
                     /locus_tag="WIE35_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ4153272.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFDRSE"
     gene            6652..7197
                     /gene="def"
                     /locus_tag="WIE35_000294"
     CDS             6652..7197
                     /gene="def"
                     /locus_tag="WIE35_000294"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ4153273.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7284..8210
                     /locus_tag="WIE35_000295"
     CDS             7284..8210
                     /locus_tag="WIE35_000295"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ4153274.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDVPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIRPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGAKL"
     gene            8289..9548
                     /gene="rsmB"
                     /locus_tag="WIE35_000296"
     CDS             8289..9548
                     /gene="rsmB"
                     /locus_tag="WIE35_000296"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ4153275.1"
                     /translation="MSMALAQKLAADSVAAVAEGRNLQDVLAEIRAAHPQLTAQENGA
                     LQDIAYGCQRYLGSLKHMLGQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLVAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9514..10113
                     /locus_tag="WIE35_000297"
     CDS             9514..10113
                     /locus_tag="WIE35_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ4153276.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10113..12230
                     /locus_tag="WIE35_000298"
     CDS             10113..12230
                     /locus_tag="WIE35_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ4153277.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     AGSFTQLSLYNAASGKIEKSINPHKLDQPFPDKARWEKIQQAGSVRDLESIGGVLYAQ
                     GWLSAGTHNGRDYALFFRQPIPKDVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLL
                     IASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKL
                     FNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGRLKTFNKAAEQ
                     ILGMSLVPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVQVEYAAPDDAKILL
                     GKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAERL
                     AWKLGGKLDEQDAQILTRSIDTIIKQVAALKEMVEAFRNYARAPSLKLENQDLNALIG
                     DVLALYEAGPCRFEAELADEPLMMAADTTAMRQVLHNIFKNAAEAAEEADMPEVRVKS
                     EAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRIS
                     LSNQDAGGACVRIILPKTVETYA"
     gene            12223..13500
                     /locus_tag="WIE35_000299"
     CDS             12223..13500
                     /locus_tag="WIE35_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ4153278.1"
                     /translation="MRSSDILIVDDEVGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKHGAAQIETGPVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPTELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13591..14778
                     /gene="dprA"
                     /locus_tag="WIE35_000300"
     CDS             13591..14778
                     /gene="dprA"
                     /locus_tag="WIE35_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ4153279.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLRRFGSASNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGEKGIPVVSGMASGIDT
                     AAHQGALQAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14804..15265
                     /locus_tag="WIE35_000301"
     CDS             14804..15265
                     /locus_tag="WIE35_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ4153280.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15344..17650
                     /gene="topA"
                     /locus_tag="WIE35_000302"
     CDS             15344..17650
                     /gene="topA"
                     /locus_tag="WIE35_000302"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ4153281.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17723..18418)
                     /locus_tag="WIE35_000303"
     CDS             complement(17723..18418)
                     /locus_tag="WIE35_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153282.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFVPEMN
                     RILSDFKTTRGFK"
     gene            complement(18438..>18807)
                     /locus_tag="WIE35_000304"
     CDS             complement(18438..>18807)
                     /locus_tag="WIE35_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153283.1"
                     /translation="KNKEILSACILCITLIAFSWAWIIIYRPNTKRFFLSLLIVWGKL
                     LLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRNLLN
                     LCCLHKDFSTPSKNIEVNRS"
BASE COUNT         4602 a         4872 c         5186 g         4147 t
ORIGIN      
        1 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat ataaaataaa
       61 tgggcaaaaa tcggtttatt atcgtttttg ccgcatttgg atttgttcta ccgtaaaacg
      121 tgtttgacga acgggattct tattaaaaaa catctgattt ctaacaaaat cagtattttt
      181 tggcacgatg gctaaaattt ttccttccat ttcgccatca cgtgttttcc atgcgctcaa
      241 gaattgtgat ttgctcattg agacgtgccc cagcgatgga tcagccagca aaacagtttc
      301 tccgttaata ccgttcaata ccgaaaaatg gttgttttta cggtatttta aatacacaat
      361 tacaggaatt tttagttgta ccaactgttc aaatggcaaa gcataacctt gtgcttcaaa
      421 acccagttcg ggcattatgc gttgcatatc atcaaaagaa gcacgcattt gggttttatc
      481 cattttgtcc aagatttccg cttcagaata atgtctgcca taaaaattat tcagtaacgt
      541 ggcaatcgaa gccgcgccgc aagaaaaatc caaatcttgt tttactatgc cggaatctcg
      601 ccgtgctttc caacttcgta catggatgtt ttggtaagaa gcggggggtc aacaaacata
      661 ggccaagcaa aaactatatt tggggcgaaa ccaatcaaag ccgcataatt tatcaattta
      721 taaagatttt ttatcataat atgtatacgc ggaataaacg catgaaacat aaaaaaataa
      781 aatcatatgc aattttattg catgaataaa tatgaataaa atagataatg atgggagtaa
      841 atacgccatg tattttggaa gtttaaattt attaataata aaataattta tcgtagcgca
      901 aataaatccc aaaattggaa cgattagaaa aaaaattaat acacccatca tcatgcaaac
      961 tctatattaa taaatagcta actaattcta ttgtggaaat tagttagcta actaaaagtt
     1021 attaatgatt atttccgaga attgactgca ttgttggcag cattggcccc aaaacctagt
     1081 gcatgaatac ccggtctcca tgccaaattc ccagccaatc cgcctccggc agcagcagcc
     1141 agccctgttt tgccgctact cctgttgccg caccgattcc tgccgcagta gccgcgcctt
     1201 gcgcagttcc taatttacca tgattataca aattagcacc atgatactcc catgcaccta
     1261 atgcaccgcc aaaagcagcg gctgcaataa tgggaacaaa ttcaccttgt gtttctttca
     1321 tttcagcctg tgataattga attgctttca cattttggct gtcaaaaact tggctgtcta
     1381 aattttgcgc cattacaggt gtaatcatca tagccattac agttgcaatt ttcgttgcgc
     1441 tggtttgcac ataaatagga ttagcaaatt cgctttgatt gcgttcagtg ttgatgtagc
     1501 taatactgct ttctagtttg aatttaccct tgtcagtcaa taattcttcc aaacttaaag
     1561 gtaagtcagc ataagcaatt tggctggcaa cagtcaaaat aaaatctatt agacatttgt
     1621 gtttttgcat catttcgttt gattttctag tttttgagaa tgatacaaag ttttttacaa
     1681 agtaaagagt cactctgaaa aacatttttt cattataaat caaaatattg atagaataaa
     1741 tagcgagcat cgattcacgg tgtactttag tgcaaagcgt acaacgcgag cctgaaccac
     1801 cagccgcaac aggaaaagaa agccgataga gtgcaatgct tgccaacgtg caagcgagct
     1861 tgcaagaacg cttggctcaa cgagagcagg caagacagaa agcagaaagc aggataggag
     1921 cggtaacgca aaggtctcgg gctttgattt cgccgtaaac cctgctgccg ccttgtccgg
     1981 aaagggcgca ggcggcgagt gccgacaggg tgcagatggg gaggggggtt ttcatttggg
     2041 gtcgcaacgg aagtggtatg cgcagatttc aaaaccgttt ttgaaataca ggcggtgcgc
     2101 gtcggcacgg tcgtggttga cgtggacgtt gaggtggatt ttggttaccc ctgtttccgc
     2161 gccgattttg cggacttctt ccaaaaggcg cgaggcgtag cctttgcggc ggctttgcgg
     2221 cagggtaacg atgtcatcga tgtggatgtg gcggccgctg gcgagggtgc aggcttcgcg
     2281 gaagccgcag acggcgacgg cattgtgttt gccttcttca aaaataccca gcaggcggta
     2341 gccttggggg cgttgggttt tgttgatctg ttcggtaaag cggttgatgt cggtcagggc
     2401 ggaacgcaaa acgctcaagg ctgcaaaggc ggtggcggtg tcgtccgcgc cgatttcgcg
     2461 caaaacgtag gatgcgcccg aggcggtctg ttcctgtgct ttctcggcgg cgtgtttttc
     2521 ttcgattgcc tgtgccagca tgacgtgttc gtcggcaggg ttgttttgtc cgccctgttc
     2581 gcgttcttcg agcagggctt tgcagtcgat gacgcgcagg tcgttgtcgg cggcaaagtc
     2641 catcaggaag cggaacattt gggggttgtc ggtttccagt tttttatcga cggcgacgca
     2701 gcggatgttg tccaccagca tggggcgcac ccatttcgaa taggaaagcc tgtggtcttt
     2761 ggtgaacgag gacagaatgc cgcacaggcg ttccgcccag tcgctgggac ggaaaatctt
     2821 gccggaactc gttgtgccgt ggatgacgac ttcgtagggg ttgcagacta acatggcggc
     2881 ttcctgaaaa gaaatgtctg gcgcgattat accttatgct tatgcgggcg tgtttggata
     2941 tgccgtctga aaagtacggg attcgtgcgg taaaactttg cggcggcaaa tgtgcgataa
     3001 tacgcgccgt attgccgctt ttgcgaagct gttccgcaaa catacgggcg gcgtggacga
     3061 cgtataaccg gatacccgcc tgacgcgggt tttttacgga agggggcaaa aatgcctaat
     3121 ccgctttaca gacagcacat catctcgatt tcggatttgt cgcgcgaaca gttggaatgc
     3181 ctgcttcaga cggcattgaa gctgaaggcg catccgcgcg gcgatttgtt ggaaggcaaa
     3241 cttatcggtt cgtgcttctt tgaaccgtcc acgcgcacga ggctgtcgtt tgaaacggcg
     3301 gtgcagcggc tgggcggcaa ggtcatcggc ttttcggacg gcgcgaatac cagtgccaaa
     3361 aaaggcgaga cgcttgccga taccgcccgc atcatttccg gctacaccga cgcaatcatc
     3421 caacgccacc ccaaagacgg cgcggcgcgc gtggcagcgg agttttcgcg cgtccccgtc
     3481 atcaacgccg gcgacggcac caaccagcac cccagccaga ccttgttgga tctggtaacg
     3541 atttatgaaa cgcagggacg tttggacaag ctcaaaatcg ccatggcggg cgacttgaaa
     3601 tacggacgta ccgtgcattc gctttgtcag gcgttgaaac gctggggctg cgaatttgcc
     3661 tttgtttcgc cgcccagcct cgcgatgccc gactacatta ccgaagagtt ggacgaagcc
     3721 ggctgccgat accgtatcct cggtagtttg gaagaagcgg cggaatgggc ggacatcctg
     3781 tatatgaccc gtgttcaacg cgaacgtttc gacgaacagg aatttgccaa aatccaaggc
     3841 aaattcaatc tcgacgcatc catgcttgcc cgcgccaaac cgaacctgcg cgtgctgcac
     3901 cctttgccgc gcgtggacga aatccatccc gatgtcgatg ctacgccgca tgcctattat
     3961 ttcgagcagg cgaccaacgg cgtttatgcg cgtatggcga tattgtcgct ggtgttgaac
     4021 gaagaagtgt gaggaaccga tatggaaacc ccgaaactca gtgtcgaagc cattgaaaaa
     4081 ggtacggtta tcgaccatat tcccgccggc agggggctga ccatcctgcg ccagttcaaa
     4141 cttttgcact acggcaacgc ggtaaccgtg ggcttcaacc tgcccagcaa aacccaaggc
     4201 agcaaagaca tcatcaaaat caaaggcgtg tgcttggacg acaaagccgc cgaccgcctc
     4261 gccctgttcg cccccgaagc ggtggtcaat accatcgaca atttcaaggt cgtgcagaag
     4321 cggcatttga acctgcccga tgaaattgcc gaagtgttcc gttgcccgaa cacgaattgc
     4381 gccggccacg gcgagccggt caaaagccgg ttttatgtta aaaaacacaa cgggcagacg
     4441 cggctgaaat gccattactg cgaaaaaacc tacagccggg attcggtggc ggaagcctga
     4501 cggattccct taaaccgagc gggcggcatt tcgtctgccg cttgttttgc caatctgaaa
     4561 tggaatgatg atgcacgctt ctgtccaaag ccgtttcgca ccgatacttt atgttttgat
     4621 tttctttgcc ggttttttga ccgcgcaaat ttggttcaat cagaaagcct atactgaaga
     4681 gctgcctccg cttctgtccg cattgtccgc cgtcgcgctg gtgtggctgg cgtgggcgtt
     4741 cgtgtcggcg cgttcaaagg ctaaggcgga aaagttctac cgcgaaaaaa tgatacagaa
     4801 cgaaagcata caccccgtcc tgcacgcttc tttgcaacac ttggaacaca agccgcaaat
     4861 gctcgccctg ctggtcaaaa accacggcaa agggatggcg gaacaggtca ggttcaaggc
     4921 ggaagtgctg cccgacgacg aagacgcgcg cacgattgcc gccgagttgg caaaaatgga
     4981 tatgtttgca ttggggacgg acgcggtcgc ctcgggcgaa acctatggac gcgtgttcgc
     5041 cgatattttc gagttgtcgg cggctttgga agggcgcgcg ttcaaaggaa tgttgaaact
     5101 gacggcggaa tataaaaaca tcttcggcga tgcctgccgt tcggaaacgg cgttggattt
     5161 gggcgcgctc aatcaggcgt tgcaggagat ttcaaaaaca tcggaaaagt ccaaacggat
     5221 attttattga agatggaaaa atgccgtctg aaacggaagg tgtttcagac ggcatttttg
     5281 tcggatgatt aattattcgg agcggtcgaa gccaaacttc acgcggctgc ggccctgatc
     5341 cggtatattg tccaaatcgc gtcccggatt ggcggcggtg tcgcctacgg aaatatcgga
     5401 gatgttttcc aaaatgatgg cggacgacag gtgttcggag gtgcggtaaa ccattgccaa
     5461 gcccacttct tcggcaggag tggaaatcag ctcgacggta tccctgcttt tgaaattgtt
     5521 ggagaggtcg acctgcatcg ttttcttgcg tttgtagagg ctcaaaaccg tgcctttgtc
     5581 caaaccgtcc gcctcgcctt tgtcgatggt gatggtttga aactggccgg caatccttgt
     5641 accttcaaac acggaaacga ttttagcctg aaccgggcgg gacggttcgt gcggcatcat
     5701 gttgaagcgg tcggtgtctt ccggcatttt catcaggtag tcgccctgct gtatttcgga
     5761 aatggcggtt tcgaccacca gcggctgtat cgaaggggtg cgcagcgggg taatcaaagg
     5821 atgggtgcgg gtatggtatt cgttgtcttt cggccgttct ccagcctgtt tcgagcgttg
     5881 ttcgaggaca gagtcggtat agtcgaggga gcgcacgatg ccgctgaatg cgacttcctg
     5941 cccgaggaat ttacccgtat ccggatcggt gatgttttta ttgattcggt aggtcaggta
     6001 gcggcccggc tctttcaggc ctttggtgta aaccctggtg cctttggtgt acagcagcct
     6061 gccttccggg cccgagagca ggcgcggcgc ggcagcggtt tctttgcggg aaacgatttg
     6121 cgggtgccgc ataaagatgc ggtagaagtt gacatcgatg gcgggaatac cgtatccgga
     6181 cacttcctta tccggactca ttttgacgac ggggatgccg tctgtctgtt ccaagccgag
     6241 gcgcggttcg ccgtcaacgt ggcgcaacac caatacctgg tccggataaa tcaggtcggg
     6301 attgtggatt tgatcccggt tcgcgtccca caggcggccc cattgccacg ggctgtacag
     6361 gtatttgccc gaaatgcccc acagggtgtc gccctgtttg accgtgtagc gttccggcgc
     6421 gttcgggcgc acctccaaat ttgccgccaa agtttgtgtt gagaatgcca tacctgccgc
     6481 gcagagcagg gttataatac gacgttgcat aaccgttccc cttatctgat aaatttcggt
     6541 ttgtcttgct tgattgggtt ggaaaaagcg gcggcagccc ctcgggatgt gccgcgtgat
     6601 aaaaaatgtt ccgcatttta acatcgaatt atccgcacca tcacggtaat tatgaaaaac
     6661 aggcggcgta tccgccgaag gaaagagaaa attatggctt tattgaatat cttgcaatat
     6721 cccgacgagc gtctgcacac ggtggcaaag cctgtcgaac aagtcgacga gcgcatccgg
     6781 aagctgattg ccgatatgtt tgaaacgatg tacgaatcgc gcggcatcgg gctggcggcg
     6841 acgcaggtcg atgtgcacga gcgcgtggtc gtgatggatt tgaccgaaga ccgcagcgaa
     6901 ccgcgcgtgt tcatcaaccc cgtcatcgtt gaaaaagacg gcgaaaccac ttacgaagag
     6961 ggctgcctgt ccgtgccggg catttacgac accgtaaccc gcgccgaacg cgtcaaggtc
     7021 gaggctttga acgaaaaagg cgaaaagttc acgctggagg cggacggctt gctggcgatt
     7081 tgcgtgcagc acgagttgga tcacctgatg ggcatcgtgt ttgtcgaacg cctgtcgcag
     7141 ctcaagcagg ggcggattaa gaccaagctg aaaaaacgtc agaaacatac gatttgaccc
     7201 ttttgccgtg ccgtctgaac gctgcaaagt tttcagacgg cacggtcttg tccgacaatt
     7261 ttacgcacac gcaggaacac gctatgaaag tcatcttcgc cggcacgccc gattttgccg
     7321 ccgccgcctt aagagccgtt gccgccgccg gttttgaaat cccgctggtg ctgacccagc
     7381 ccgaccgtcc gaaagggcgc ggtatgcaac tgactgcccc gcccgtcaaa caagccgcgc
     7441 tggaactcgg tttgcgcgtc gaacagcccg aaaaactgcg taacaacgcc gaagccctgc
     7501 aaatgctcaa agaggtcgag gcagacgtaa tggtggttgc cgcctacggt ttgattctgc
     7561 cgcaagacgt gttggatgtg ccgaaacacg gctgcctcaa tatccacgcc tcgctgctgc
     7621 cccgttggcg cggcgcggcg ccgattcagc gcgcgattga ggcaggcgat gccgaaaccg
     7681 gtgtgtgcat tatgcagatg gacatcggtt tggacaccgg cgatgtggtc agcgaacacc
     7741 gctacgccat ccgacctacc gataccgcca acgaagtcca cgacgcgctg atggaaatcg
     7801 gtgcggcggc ggttgttgcc gatttgcaac agcttcaaag caaaggccgt ctgaacgcgg
     7861 tcaaacagcc cgaagaaggt gttacttacg cccaaaaact aagcaaagaa gaggcgcgta
     7921 tcgattggag cgaaagcgcg gcggttatcg aacgcaaaat ccgcgccttc aaccccgtgc
     7981 ctgccgcgtg ggttgagtat cagggcaagc cgatgaaaat ccggcgggcg gaagtggtgg
     8041 cgcaacaagg cgcggcaggc gaagtgttgt cctgttcggc ggacggtttg gtcgttgcct
     8101 gcggcgaaaa cgcgctgaag attaccgaat tgcagcctgc cggcggcagg cggatgaata
     8161 tcgcggcgtt tgcggcggga cggcatatcg aagcaggggc gaagctgtaa atcccttcag
     8221 acggcattcc gatccgcaaa cgggaatgcc gtctgaaacc atcagtcgaa gaaagcgaat
     8281 cacataatat gagtatggca cttgcccaaa aacttgccgc cgacagcgtt gccgcggttg
     8341 ccgaagggcg caatcttcag gatgttttgg cggaaatccg cgcggcgcat ccgcagctga
     8401 cggcgcagga aaacggcgcg ttgcaggata ttgcctacgg ttgccaacgt tatttgggca
     8461 gtttgaagca catgcttggt caaatgctga agaagccgat tgacaatccg cagctcgaaa
     8521 gcctgctttt ggcagcgttg taccaactgc attacacgcg caacgcgccg cacgccgtgg
     8581 tcaacgaagc tgttgaaagc atcgccaaaa tcggacgcgg gcagtaccgt tcgtttgcca
     8641 acgcggtttt gcgccgcttt ttgcgcgaac gcgacaagct tgcagcttcc tgtaaaaaag
     8701 acgatgtggc gaaacacaac ctgccgctgt ggtgggtggc ttacctgaaa aaccattatc
     8761 cgaaacactg gcacaacatc actgccgcgc tgcaatccca tccgccgatg acgttgcgcg
     8821 tcaaccgccg acacggcaat gccgaaagct atttggaaaa actggtggcg gaaggtatcg
     8881 cggctaaggc gttggacgaa tatgcggtta cgttggaaga agccgtgccg gtaaaccgcc
     8941 tgcccggttt ttcagacggc atcgtttcgg tacaggactt cggcgcgcag caggcggcgt
     9001 atctccttaa cccgaaagac ggagaacgga ttttggacgc gtgcgccgcg ccgggcggca
     9061 agacggggca tatcttggaa ctggcggatt gccgcgttac cgccttggac atcgatgcag
     9121 gccgtctgaa gcgtgtggag gacaatatcg cgcgcctggg ctttcagacg gcatcggcgg
     9181 cgtgtgccga tgcgcgggat ttggcggcgt ggtatgatgg aaagcctttc gacgcaatcc
     9241 ttgccgacgt gccatgcacc gcttccggcg tggcgcggcg caatcccgac gtgaaatggc
     9301 tgcgccgtcc gaccgacgcg ctcaaaaccg cccgccagca ggaagccctg ctggacgcac
     9361 tttggcagac gctgaaacag ggcggcagaa tgctgcttgc cacctgttcc gtgttcgtcg
     9421 aagaaaacga cgggcaattg caaaaattcc tcaaccgcca tgccgatgcc gaaccgatcg
     9481 aatcgcgggt gcttttaccg aacaaacacc aagatggctt ttattacgcg cttattcaaa
     9541 agcattaaac aatggcttgt gctgctgccg atgctttccg ttttgccgga cgcggcggcg
     9601 gaggggatag atgtgagccg cgccgaagcg aggataaccg acggcgggca gctttccatc
     9661 agcagccgct tccaaaccga gctgcccgac cagctccaac aggcgttgcg ccggggcgtg
     9721 ccgctcaact ttaccttaag ctggcagctt tccgccccga taatcgcttc ttatcggttt
     9781 aaattggggc aactgattgg cgatgacgac aatattgact acaaactgag tttccatccg
     9841 ctgaccaacc gctaccgcgt taccgtcggc gcgttttcga cagactacga caccttggat
     9901 gcggcattgc gcgcgaccgg cgcggttgcc aactggaaag tcctgaacaa aggcgcgctg
     9961 tccggtgcgg aagcagggga aaccaaggcg gaaatccgcc tgacgctgtc cacttcaaaa
    10021 ctgcccaagc cttttcaaat caatgcattg acttctcaaa actggcattt ggattcgggt
    10081 tggaaacctc taaacatcat cgggaacaaa taatgcgccg ttttctaccg atcgcagcca
    10141 tatgcgccgt cgtcctgttg tacggactga cggcggcaac cggcagcacc agttcgctgg
    10201 cggattattt ctggtggata gtcgcattca gcgcaatgct gctgctggtg ttgtccgccg
    10261 ttttggcacg ttatgtcata ttgctgttga aagacaggcg cgacggcgta ttcggttcgc
    10321 agattgccaa acgcctttcc gggatgttca cgctggtcgc cgtattgccc ggcgtgtttc
    10381 tgttcggcgt ttccgcacag ttcatcaacg gtacgattaa ttcgtggttc ggcaacgaca
    10441 cccacgaagc cctcgaacgc agcctcaatt tgagcaagtc cgcattgaat ctggcggcag
    10501 acaacgccct cggcaacgcc gttcctgtgc agatagacct catcggcgcg gcttccctgc
    10561 ccggggatat gggcagggtg ctggaacatt acgccggcag ctttacccag ttgtcccttt
    10621 acaatgccgc aagcggcaaa atcgaaaaaa gcatcaaccc gcacaagctc gatcagccgt
    10681 ttccggataa ggcgcgttgg gaaaaaatcc aacaggcggg ttcggtcagg gatttggaaa
    10741 gcataggcgg cgtattgtac gcgcagggct ggctgtcggc aggtacgcac aacgggcgcg
    10801 attacgccct gtttttccgc cagccgattc ccaaagacgt ggcagaggat gccgtcttaa
    10861 tcgaaaaggc gcgggcgaaa tatgccgagt tgagttacag caaaaaaggt ctgcagacct
    10921 ttttcctggc taccctgctg attgcctcgc ttttatcgat ttttcttgcg ctggtgatgg
    10981 cattgtattt tgcccgccgt ttcgtcgaac ccgtcctatc gcttgccgag ggggcgaagg
    11041 cggtggcgca aggcgatttc agccaaaccc gtcctgtttt acgcaatgat gagttcgggc
    11101 gtttgaccaa actgttcaac catatgaccg agcagttgtc catcgccaaa gaagcggacg
    11161 agcgcaacag gaggcgcgag gaagccgcca gacattatct cgaatgcgtg ttggaggggc
    11221 tgaccacggg cgtggtggtg tttgacgaac aaggccgtct gaaaaccttt aacaaagcgg
    11281 cggaacagat tttgggtatg tcgctcgtgc cgttgtgggg cagcagccgg cacggttggc
    11341 acggcgtttc ggcgcagcag tccctgcttg ccgaagtgtt tgccgccatc ggcgcggcgg
    11401 caggtacgga caaaccggtc caagtggaat atgccgcgcc ggacgatgcc aaaatcctgc
    11461 tgggcaaggc gacggtattg cccgaagaca acggcaacgg cgtggtgatg gtgattgacg
    11521 acatcaccgt attgatacac gcgcaaaaag aagcggcgtg gggcgaagtg gccaaacgtc
    11581 tggcacacga aatccgcaat ccgctcacgc ccatccagct ttccgccgaa cggctggcgt
    11641 ggaaattggg cgggaagctg gacgagcagg acgcgcaaat cctgacgcgt tcgatcgaca
    11701 ccatcatcaa acaggtggcg gcgttaaaag aaatggtcga agcattccgc aattacgcgc
    11761 gcgcgccgtc gctcaaattg gaaaatcagg atttgaacgc cttaatcggc gatgttttgg
    11821 ctctgtacga agccggcccg tgccggtttg aggcggaact tgccgacgaa ccgctgatga
    11881 tggcggcgga tacgaccgcc atgcggcagg tgctgcacaa tattttcaaa aatgccgccg
    11941 aagcggcgga agaagccgat atgcccgaag tcagggtaaa atcggaagcg gggcaggacg
    12001 gacggattgt cctgacggtt tgcgacaacg gcaaagggtt cggcagggaa atgctgcaca
    12061 acgccttcga gccgtatgta acggacaaac cggcgggaac gggattggga ctgcccgtgg
    12121 tgaaaaaaat cattgaagaa cacggcggac gcatcagcct gagcaatcag gatgcgggtg
    12181 gggcgtgtgt cagaatcatc ttgccaaaaa cggtagaaac ttatgcgtag cagcgatatt
    12241 ttaattgtag acgacgaagt cggcatccgc gacctgctgt cggaaatcct gcaggacgaa
    12301 ggctattcgg tcgcattggc ggaaaatgcc gaagaggcgc gcaagctgcg ccatcaggcg
    12361 cgtcccgcga tggtgctgct ggatatttgg atgcccgatt gcgacggcat cacgcttttg
    12421 aaggagtggg cgaaaaacgg gcagctcaat atgccggtgg tgatgatgag cgggcatgcc
    12481 agcatcgata ccgccgtaga agcgaccaaa atcggcgcga tcgatttttt ggaaaaaccg
    12541 atttccctgc aaaagctgct gtctgccgtc gaaaacgcgt tgaaacatgg tgcggcgcaa
    12601 atcgaaacgg gaccggtgtt cgacaagctg ggcaacagtg ccgcgattca ggagatgaac
    12661 cgcgaagtcg aagcggcggc aaagcgcacg tcccctgtat ttttgacggg cgaagcaggt
    12721 tcgccgtttg aaaccgtggc gcgctatttc cataaaaacg gtacgccgtg ggtcagtccg
    12781 gcaagggtcg aatatctgat tgatatgccg acggaactgt tgcagaaagc tgaaggcggt
    12841 gttttatatg tgggcgatat tgcccaatac agccgcaaca tccaagccgg cattgccttt
    12901 attgtcggca aggcggaaca ccgccgcgtc agggtggtcg catcgggcag cagggcggca
    12961 ggttcagacg gtattgcctg cgaggaaaag ctggcggaac tgctgtcgga gtcggtcgtc
    13021 cgcattccgc cgctgcgtat gcagcatgaa gatattccct tcctgataca ggggattgcc
    13081 tgcaatgtgg cggaaagtca aaagattgcg cccgccgcat tcagtgaaga ggcacttgcc
    13141 gcattgaccc gttacgactg gccgggaaat ttcgaccaac tgcaaagcgt cgttgcaacg
    13201 ctgttgttgg aggcggacgg acaggaaatc ggcgcggcgg cggtttcttc ccttttgggg
    13261 cagaatgtgc ctgccgaggg agcggaagat atggtgggcg ggtttaattt caacctgccc
    13321 ctgcgcgaat tgagggagga ggtggagcgg cgttatttcg agtaccacat cgcccaagaa
    13381 ggtcagaata tgagcaaagt ggcgcagaaa gtcggtttgg aacgcacgca cctttaccgc
    13441 aaactcaaac agctcggcat cggcgtttcg cgccgggcgg gggaaaaaac cgaagaatag
    13501 gcccggacgg ccggttttcc ggctgcgggc ttttgttttc agacggcatt tggtgcaaat
    13561 gccgtctgaa atcgtaaggg gacggatttt atgacagagg acgaacgttt cgcgtggctg
    13621 caattggcgt ttacgcccta tatcggcgcg gaaagcttcc tgctgctgct gcgccgtttc
    13681 ggcagcgcgt caaacgccct gtccgcaccg gcggaacagg tcgccccggc ggtgcggcac
    13741 aagcaggcgg ccgaggcttg gcgcaatgcg gaaaaacgcg ctttggcgcg gcaggcggca
    13801 gaagcggcgt tggaatggga aatgcgggac ggatgccgcc tgatgctgct tcaggatgaa
    13861 gatttccccg aaatgctgac gcaggggctg accgcgccgc cggttttgtt tttgcgcggc
    13921 aacgtgcggc tgctgcacaa gccttccgcc gccatcgtcg gcagccgcca tgccacgccg
    13981 caggcgatgc ggattgccaa agatttcggc agggcgttgg gagagaaagg cattcccgtt
    14041 gtgtcgggta tggcttcggg catcgatacc gccgcccatc agggtgcgtt gcaggcggaa
    14101 ggcggcacca tcgccgtgtg ggggacgggc atagaccgca tttatccgcc gtccaataaa
    14161 aaccttgcct atgaaatcgc cgaaaaagga ctgattgtca gcgagttccc catcggcacg
    14221 cgcccgtatg ccggcaattt tccgcgccgc aaccgcctga ttgccgcgct gtcgcaggtt
    14281 acgctggtgg ttgaagccgc cttggaatcc ggttcgctga ttaccgccgg cttggcggca
    14341 gaaatggggc gcgaagtgat ggcggtaccc ggctcgatag acaatccgca cagcaaaggc
    14401 tgccacaaac tgattaaaga cggcgcgaaa ctgacggaat gtttggacga catcctgaac
    14461 gaatgcccgg ggctattgca aaatacgggt gcttcatcat attctataaa taaggatacg
    14521 cccgacacgg gccgccgcgc cgttcagacg gcatacgccc cgccgcctgc cgcaaaaatg
    14581 ccgtctgaag gggcggcagg cggcacagcc gtcggcggca tactggataa aatgggtttc
    14641 gaccccatcc atcccgacgt gcttgccgga cagttggcta tgcctgccgc agatttgtat
    14701 gccgcactgt tggaattgga attggacggc agcgttgccg caatgcccgg cggcagatac
    14761 cagcgtatcc gaacttgaac gcactttata ttaaggaaca cgaatgaccg aagtcatcgc
    14821 ctacctcatc gaacatttcc aagatttcga tacctgtccg ccgcccgaag acttgggtat
    14881 gctgcttgaa gaagcgggtt tcgatacgat ggaaatcggc aacaccctga tgatgatgga
    14941 agtattgctc aacagctccg aattttccgc cgaacctgcc gacagcggcg cattgcgcgt
    15001 gtacagcaaa gaagaaaccg acaacctgcc gcaggaagtg atggggctga tgcagtatct
    15061 gattgaagaa aaagccgtca gctgcgaaca gcgggaaatc atcatccacg cgctgatgca
    15121 cattccgggc gacgaaatta ccgtagatac cgccaaagtg ctgaccctgc tgcttttatg
    15181 ggcaaacaag agcgagctgc ccgtgttggt cggcgacgag ctgatgagcg cgcttttact
    15241 cgacaacaaa cccacgatga actgaagcgg cttcagacgg cattatcctg ctaacctgcc
    15301 gtctgaaacg ccggcatcaa aaccaccatc cagagaacga caaatggcga aaaacctatt
    15361 aatcgtcgaa tccccgtcca aagccaaaac cctgaaaaaa tatttgggcg gcgattttga
    15421 aatccttgca tcctacggac acgtccgcga cctcgtcccc aaaagcggcg cggtcgatcc
    15481 cgacaacggc tttgcgatga aataccaact catcagccgc aacggcaaac acgtcgatgc
    15541 catcgtcgcc ggtgccaaag aagctgaaaa catctacctc gccaccgacc cggataggga
    15601 aggcgaagcc atttcctggc atcttttgga aatcctcaaa tccaaacgcg gcttgaaaaa
    15661 catcaagccg cagcgtgtcg tgttccacga aatcaccaaa aacgccgtgc tcgatgccgt
    15721 tgcccatccg cgcgaaatcg aaatggactt ggtggatgca caacaagccc gtcgcgcttt
    15781 ggactatttg gtcggcttta acctttcgcc attgttatgg aaaaaaatcc gccgcggttt
    15841 gagcgcaggc cgcgtgcaaa gccccgcttt gcgcctgatt tgtgagcgcg aaaacgaaat
    15901 ccgcgcgttt gaagcgcagg aatattggac ggtgcatctc gacagccaca aaggccgcag
    15961 caagttcacc gccaaactcg cccaatacaa cggcgcgaaa ctcgaacaat tcgacctgcc
    16021 gaacgaagcc gcgcaagccg acgtgttgaa agaactcgaa ggcaaagagg ccgtcgttac
    16081 cgccattgaa aagaaaaagc gcagccgcaa tcctgccgcg ccgttcacca cttctaccat
    16141 gcagcaggat gccgtgcgca aactcggctt caccaccgac cgcaccatgc gtaccgccca
    16201 acagctttat gaaggtatag acgtagggca gggcgcgatc ggtctgatta cctatatgcg
    16261 tactgacagc gtgaatttgg cggatgaagc gttaaccgaa atccgccatt acatcgaaaa
    16321 caaaatcggc aaagaatatc tgccgagtgc cgccaaacaa tacaaaacca aatccaaaaa
    16381 cgcccaagaa gcgcacgaag ccatccgtcc gacttccgtg taccgtacgc ccgaaagcgt
    16441 caaacccttc ctgagcgcag accagttcaa actctatcaa atgatttggc agcgtaccgt
    16501 cgcctgtcag atgacgcctg ccaaattcga ccaaaccacc gtcgatatta ccgtcggcaa
    16561 aggcgtattc cgcgtaaccg gacaagtgca aaccttcgca ggcttcttga gcgtttatga
    16621 agaaagcagc gacgatgaag aaggcgaaga cagcaaaaaa ctgcccgaaa tgagcgaagg
    16681 cgataaattg cccgtggaca aactctacgg cgaacaacac tttaccactc cgccgccgcg
    16741 ctacaacgaa gccacgctgg ttaaagccct cgaagaatac ggcatcggcc gcccctcgac
    16801 ctacgccagc atcatctcca cgctcaaaga ccgcgaatac gttacccttg agcaaaaacg
    16861 cttcatgccc accgacacag gcgacatcgt caacaaattc ctgaccgaac acttcgccca
    16921 atacgtcgat taccacttca ccgccaaact cgaagaccag cttgacgaaa ttgccgacgg
    16981 caaacgccaa tggattccct tgatggacaa attctggaaa ccgttcatca aacaagtgga
    17041 agaaaaagaa ggcatcgaac gcgccaaatt taccacgcag gaacttgatg aaacctgccc
    17101 gaaatgcggc gaacacaaac tgcaaatcaa attcggcaaa atgggtcgtt ttgttgcgtg
    17161 tgccggttat cccgagtgca gctacacgcg caatgtcaac gaaaccgccg aagaggccgc
    17221 cgagcgcatc gccaaagccg aagccgaaca ggccgaactc gacggacgcg agtgcccgaa
    17281 atgtggcggt cgcctagtgt acaaatacag ccgcaccggc agcaaattca tcggctgcgc
    17341 caactatccg aaatgcaaac acgtcgagcc gctggaaaaa ccgaaagata ccggcgtcca
    17401 gtgtccgcaa tgcaaaaaag gcaacctcgt cgagcgcaaa tcccgctacg gcaaactgtt
    17461 ttacagttgc agcacctatc ccgactgcaa ctacgccact tggaacccgc ccgttgccga
    17521 agaatgcccg aactgccatt ggccggtctt gaccatcaaa accaccaaac gctggggcgt
    17581 cgaaaaagtc tgcccgcaaa aagaatgcgg ctggaaagaa cagattgaac cgcccgcgcc
    17641 gaaggagtaa gattaggttg gtttgaaaga gaaaaggtcg tctgaaaaat tttcagacga
    17701 cctttgcttt tctgtgattg gtttatttga atccgcgtgt tgttttaaag tccgataaaa
    17761 tccggttcat ttcaggcaca aacaaggcga tgtaatcgta agatagaccg cgactggcac
    17821 tgggatgggg aaagcagacg acttcgcaat cttcaaacga ttggaatttg acattgaaac
    17881 gtgtaccgtc aaattctttt tgcaccgtct ccagcggttt ggtctgctta ccgaccaact
    17941 gctcgaagcg tggcagtaca ttttggttgt tcagaaaatc cgccaacctg ctgcccatga
    18001 agaggatgac tttcggacgc agtttttcga tgtggtagag aaaattatcg atgtgctcgg
    18061 gttgtgtgaa cttgtcggga ttgtcgatag tgttgccctg tgtagcagcc cagttggttt
    18121 gaaccaggga tttttcaaat gcaccgccca atccattttc gtctaagggg tgtccccaca
    18181 tttcaaacca attttttatc gtattgtcgt aacgccactt ttttgcctgc tctccgaaat
    18241 agagggattt gtttgcaaat gtatggtcga ttttgttttc agggagtttg tattcacctg
    18301 ctacataagc agcctcatcg gctttactcc aaccccattc atagccacaa atcattaagc
    18361 catgtttgtc gttgtagcct ttgaacaggc tgttgctcaa attcaaatcc ttcatcatga
    18421 actcttcctt ttaaaattta agagcgattg acttcaatgt ttttagatgg ggtggaaaaa
    18481 tccttgtgta ggcaacataa attcaataaa tttcttgata attcgaaacc tactaatagc
    18541 gcacctataa aagctttttc attacgttca gcatgacggt cacgtcgttc atatttttta
    18601 cgcttgctgt tccctgttat tacagctaag ccaagtgata tggcgagaat tgcccaaaca
    18661 atagtactta ataacaattt tccccatact atcaataggg aaagaaaaaa ccttttagta
    18721 ttaggtcgat agattataat ccatgcccat gaaaatgcaa taagagttat acataagata
    18781 caagctgata ggatttcttt attttta
//
LOCUS       DAWHXZ010000013        18585 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000013 DAWHXZ010000000
VERSION     DAWHXZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.013"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..671)
                     /gene="hpnD"
                     /locus_tag="WIE35_000305"
     CDS             complement(<1..671)
                     /gene="hpnD"
                     /locus_tag="WIE35_000305"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ4153284.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDDQTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMRRFDVPASVILQCSPTGNFAELMAFQIKRARETYCE
                     AVSLLP"
     gene            842..2704
                     /gene="hscA"
                     /locus_tag="WIE35_000306"
     CDS             842..2704
                     /gene="hscA"
                     /locus_tag="WIE35_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ4153285.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGGNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMPIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMSNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLEAEELQQI
                     RQGIADLQGRVKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            2780..3427
                     /locus_tag="WIE35_000307"
     CDS             2780..3427
                     /locus_tag="WIE35_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ4153286.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRTELFAVDENLVKRLFLSLRGEIVFPKNETA
                     ESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSKIRL
                     FKEAYLRKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPR"
     gene            3463..4224
                     /locus_tag="WIE35_000308"
     CDS             3463..4224
                     /locus_tag="WIE35_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ4153287.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESICV
                     PVDDISDWMFVCNGIPYGGFTVQAMRGQMTEEERTEHDAAWGIDFGDPGQILLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNSDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGASPASTTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            4280..4621
                     /gene="fdx"
                     /locus_tag="WIE35_000309"
     CDS             4280..4621
                     /gene="fdx"
                     /locus_tag="WIE35_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ4153288.1"
                     /translation="MPKITVLPHATLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(4908..5495)
                     /locus_tag="WIE35_000310"
     CDS             complement(4908..5495)
                     /locus_tag="WIE35_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ4153289.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLSGSGGGFT
                     VTAESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            5708..5905
                     /gene="iscX"
                     /locus_tag="WIE35_000311"
     CDS             5708..5905
                     /gene="iscX"
                     /locus_tag="WIE35_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ4153290.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(5973..6374)
                     /locus_tag="WIE35_000312"
     CDS             complement(5973..6374)
                     /locus_tag="WIE35_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ4153291.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            6585..7544
                     /locus_tag="WIE35_000313"
     CDS             6585..7544
                     /locus_tag="WIE35_000313"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ4153292.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            7612..8922
                     /gene="tilS"
                     /locus_tag="WIE35_000314"
     CDS             7612..8922
                     /gene="tilS"
                     /locus_tag="WIE35_000314"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ4153293.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKICVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHLLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDKLRFLKDRQISGNLR
                     EILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPA
                     LRPFWPVVADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF"
     gene            8973..9758
                     /locus_tag="WIE35_000315"
     CDS             8973..9758
                     /locus_tag="WIE35_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ4153294.1"
                     /translation="MKHISSANNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEAKMPSEEVRKLTAVLPEDGVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVVLGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLARYEDRVFATALREEKQAVLYNEDLREPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            9768..9965
                     /locus_tag="WIE35_000316"
     CDS             9768..9965
                     /locus_tag="WIE35_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153295.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            10235..10579
                     /locus_tag="WIE35_000317"
     CDS             10235..10579
                     /locus_tag="WIE35_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153296.1"
                     /translation="MRIFYDPTRAIYDSGADYLTREKHRFVVIANSAWGLLLNLSCYY
                     DKVLEKRKIPFGKQEIDDDMDKVSAHKRKFKDISEIKVGDGWEYPFNYEQGMKELDEV
                     LLKYIPFFEEER"
     gene            10591..10896
                     /locus_tag="WIE35_000318"
     CDS             10591..10896
                     /locus_tag="WIE35_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153297.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            10958..12334
                     /gene="mpl"
                     /locus_tag="WIE35_000319"
     CDS             10958..12334
                     /gene="mpl"
                     /locus_tag="WIE35_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ4153298.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     KAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKNVKRRMEIKGTVNGI
                     TVYDDFAHHPTAIETTIEGLRQRVGNARILAVLEPRSNTMKLGTMKSALPESLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            12541..13593
                     /gene="bioB"
                     /locus_tag="WIE35_000320"
     CDS             12541..13593
                     /gene="bioB"
                     /locus_tag="WIE35_000320"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ4153299.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
                     GEVAEVEKASGIKVDY"
     gene            complement(13672..14306)
                     /locus_tag="WIE35_000321"
                     /pseudo
     CDS             complement(13672..14306)
                     /locus_tag="WIE35_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            14629..14889
                     /locus_tag="WIE35_000322"
     CDS             14629..14889
                     /locus_tag="WIE35_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ4153300.1"
                     /translation="MTKAVRRKNVQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(14952..16811)
                     /gene="ilvD"
                     /locus_tag="WIE35_000323"
     CDS             complement(14952..16811)
                     /gene="ilvD"
                     /locus_tag="WIE35_000323"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ4153301.1"
                     /translation="MPDYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPHSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDIAQIER"
     gene            complement(16983..>18585)
                     /gene="cysI"
                     /locus_tag="WIE35_000324"
     CDS             complement(16983..>18585)
                     /gene="cysI"
                     /locus_tag="WIE35_000324"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ4153302.1"
                     /translation="HGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIE
                     LDKFARENSYYRSIRLTNRQTFQFHGVPKTKLQTMHRLLHKLGLDSIATAADMNRNVL
                     CTSNPIESALHQQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGK
                     TYLPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGGGQLAGFNVLAGGGLSMEHGNTKT
                     YPNISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVE
                     RRMGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEI
                     AKIHKGDFRITANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPT
                     CPLAMAEAERVLPDFIDDLDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKA
                     VGRYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRT
                     GIVKPVLNAPVDFWDASKAVAIARA"
BASE COUNT         4321 a         4833 c         5185 g         4246 t
ORIGIN      
        1 ggcagcagcg atacggcttc gcagtaggtt tcacgggcgc gtttgatttg gaacgccatc
       61 aattcggcaa aattgcccgt cgggctgcat tgcaaaatca cgcttgcggg tacgtcaaac
      121 cgccgcattt cctccatcgg caggtaaatc cgtcccctgc gcgcgtcttc gccgacatcg
      181 cggatgatgt tggtcagttg cagcgcaagt cccatcttgt cggcgtattc cagcgtttgg
      241 tcgtctgaaa accccaaaat ccgcgcaatc aggcagccga ccacgcctgc gacgcggtgg
      301 caatacagtt tcaattcttc aaaactgccg taacgggctt gaaccaaatc catctgcatc
      361 ccgtcgatta aggcttccag ttcatatttc ggcagcttga aggtttcctt aacttgccgc
      421 aaggcctgat tgacggggtg ttccggcatc gcgccgccga ataccttgtc caaatcgccg
      481 cgccaccagt tcaatgtcgc ctgtgcaaca tcggggttgg aacattcgtc aaccacatcg
      541 tccaattcgc ggcaaaaagc atataaaacc gttaccgcat cccgtttttc ctgagtcagg
      601 aaacggaagc ccgacaaaaa actggagcgg ctttcttctg ctttttggcg gcaatagtcg
      661 agtcctttca cgatttatat tcctaatgat gggcgggaaa ggcggatttt atcggcattt
      721 ggcggtagag ggcaatttcg gcggcacgac ctaatcctta gcggtttgct caactatcgg
      781 cgcaaattct gttaaaatgc cgctttcctt cctttacaca ccgcaccgac aggcagaatt
      841 tatggctctt ttgcagattt cagaaccggg tatgtccgcc gccccgcacc ggcaccgttt
      901 ggcggcaggc atcgatttgg gtacgaccaa cagcttggtc gccaccgtcc gcagcggcag
      961 tgccgcctgc ctgcccgatg ccgaagggcg cgttaccctg ccttccgtcg tccgctatct
     1021 ggaaaacggc ggcattgaag tcggcaaaac cgccctgtcc gcccaaaaaa ccgacccgct
     1081 gaacaccgtc agctccgcca aacgccttat cgggcggacg cttgccgatc tgcatcaaaa
     1141 tacgcactac ctgccttacc gtttcggcgg caatcaacgc gttatcgaac tgcatacgcg
     1201 gcagggggtg aaaacgcctg tcgaagtgtc ggcggaaatc ctcaaaaccc ttaaatcgcg
     1261 cgccgaagaa accttgggcg gcgatttggt cggcgtggtg attaccgtcc ccgcctattt
     1321 cgacgatgcc caacgccagg ccaccaaaga tgccgcgcgt ctggcgggtt tgaacgtatt
     1381 gcgcctgctc aacgaaccca ccgccgccgc aatcgcctac gggctggaca acgcctcgga
     1441 aggcacgttt gtcgtgtacg acttaggggg cggcacattc gacgtatccg tattgcaact
     1501 gaccaaagga ctgtttgaag tcaaagccac cggcggcaac agcgcgttgg gcggcgacga
     1561 tttcgatcac cgcctgttct gccgcctgct cgaacaaaac ggactctccc aactcaacga
     1621 acaagacagc caactcctgc tctcgctcgt ccgcgccgcc aaagaacaat taaccacgca
     1681 aaccgaagcg cgcattcagg cgacgctttc agacggcatg ccaatcgaca caagcatcag
     1741 tcgcgccgag ttccacaacc tgacgcagca tttggtgatg aaaacgctcg aaccggtcac
     1801 acaggcgttg aaagatgccg gtgtcggtaa aaacgaagtc aaaggcgtga ttatggtcgg
     1861 cggttcgacc cgtatgctgc acgtccaaca ggcagtcgcc accttcttcg gacaaacccc
     1921 gctgaacaac ctcaatcccg acgaagtcgt cgcgctcggc gccgccatac aggcaaacgt
     1981 cctcgcaggc aacaaaaccg acggcgaatg gctgctgctg gacgttacac ctttgtcgct
     2041 cggtttggaa acctacggcg gcttggcgga aaaaatcatc ccgcgcaatt ccaccatccc
     2101 caccgcgcgc gcgcaggact ttactacctt caaagacggt cagaccgcga tgacgataca
     2161 cgtcgtacaa ggcgagcgcg agctcgtttc cgactgccgc agccttgcca aattcaccct
     2221 gcgcggcatt ccgcctatgg cggcgggtgc ggcgcgtatc cgcgtaacct tccaaatcga
     2281 tgcggacggt ctgctgtccg tttccgccca agaacaaagc accggcgtac aggcgcaaat
     2341 cgaagtcaaa ccttcctacg gcttggacga cgacaccatc acccaaatgc tcaaagacag
     2401 catgagcaac gccgccgaag acatggcggc acgtgcccga gccgaagccg tggtcgaagc
     2461 cgaaagcctg accgatgccg tcaacgccgc cctcgagttg gacagcgatt tgctggaagc
     2521 cgaagaattg caacagattc ggcaaggcat cgccgatttg caaggccgtg tgaaagacgg
     2581 aaaagccgaa gacatccgtg ccgccgccgc caaactcggc agtattaccg acaatttcgc
     2641 cgccaaacgc atgaaccgca acatccaacg cgcgttgact ggtcaaagcg ttgataatat
     2701 ttgacggcaa atggtttcag acggcataga tataacctga taccgtctga aggctcgaaa
     2761 acacttgaaa aacatcgata tggaaaagtc aggcattgtc tatctgatga aaaccgtcat
     2821 caagggcgtg tataaaatcg gcatttcgga cgtaagcaat tttgaaggca gaatgcgcca
     2881 tttggaaaac aacggctatg cgaacgttgc cggattggaa cgcatcctcg ccgtcaaaac
     2941 cgacaattac aaagaaaaag aaaacctgct ccatgaaatt tttagtaaaa gcaggaccga
     3001 attgttcgcc gtggacgaga atcttgtaaa acgtttgttt ttatcgcttc gcggcgaaat
     3061 cgtgttcccg aaaaacgaaa cggcggaatc ggaatttgaa aaaagcgtcc acgaacgcag
     3121 gcaggaaggg aatgccggat ccggtcgcaa acaactgctt gatttggtac ggcgcggaca
     3181 ccgggaatac ccttacgcgc tgccccggct tttggcgggc gcggcatcct acaagccgaa
     3241 aaaatcgaaa atccgccttt ttaaagaagc atatttacgc aaaagcggta cgaggctgac
     3301 cgacgaaatt gcagacggca tccatattta cacctgtttt tcgcgggcgg atttggaaaa
     3361 agcctattcc gaatatttgg aacttttcaa atccgaatcg gatgccgaag gcagaaaacc
     3421 acggtaaggt gcaaacagat accgtacacg ttgaggagca gtatgatggg cgattccgtc
     3481 atttattatg tagaacaggc agacgaaccg gtaaaccgtg ccggcgaacg cgcccgtaaa
     3541 acattcaaat acttttggcg cgagcttttt tgggaacgcc gccgcatcat tcccgcctta
     3601 gattttgcca tggtcaaagc cccttttttc caagacggcg aagacggcga aatttgcgaa
     3661 catatgtgga tcgacgatat ttattttgac ggtctttaca tttatggcgt gctgaacaat
     3721 gaacccggcg aactgaccaa tgtcgaacaa ggcgaaagca tttgcgttcc ggttgacgac
     3781 atcagcgact ggatgttcgt gtgcaacggc attccctacg gcggctttac cgtacaggca
     3841 atgcgcgggc agatgacgga agaggagcgc accgaacacg atgccgcatg gggaatcgat
     3901 ttcggcgatc ccgggcagat attgctggtg tatgaagaaa aagaacatcc cgaaaatctg
     3961 gaagagcatc cgatgtgccg gaactgtatt gacgattttc ggcaacagtt gtcccaaaat
     4021 tcggattttc tgcatgagca ggacgaagac ggctatacgc cgcttcatca tgaagccatc
     4081 gcaggaaatg cacttatggt tcaagccatg cttgaatatg gcgcaagtcc tgcctcaacg
     4141 acatcggaag gctataccgc cctcgatttt gcccgcctgg cgggctggca aaatgttgcc
     4201 gacctgctcg aaccgagaca ttaggcagac agttttccga aaaacggacg caaccacttt
     4261 ttacagaaag acaataaaaa tgcccaaaat taccgtactt ccacacgcga cgttatgccc
     4321 cgagggtgca gtcatcgata acgcgccgga aggcaaaacc gtcctcgacg tactgctcga
     4381 ccacgacatc gaagtcgatc acgcctgcga aaaatcctgc gcctgcacca cctgccacgt
     4441 catcatccgc aaaggtttcg acagtttgga agaaccgacc gaattggaag aagacctgct
     4501 cgaccaagca tggggtttgg aagccgattc gcgcctgagt tgtcaggcgg ttgtcgccgg
     4561 cgaggatttg attgtggaaa tccccaaata caccatcaac cacgcgcgcg aagaacactg
     4621 aaaacaggcc gtctgaagcc ggcacgcttc agacggcatt gttgcgcgga taaggcgcaa
     4681 tcgcccgaaa acaggcgttc gtacaggcgg aactttcgat tctatagcga attaacaaaa
     4741 atcaggacaa ggcggcgagc cgcagacagt acaaatagta cggcaaggcg aggcaacgct
     4801 gtaccggttt aaatttaatt cactatatat tgatttttat cggttttctg acggaacgga
     4861 ttggtgcggc atccgaggcg gattactcgg gcgcgatgca cgggtattta tcggttttgc
     4921 agccggaaaa accgccggcg ggttactttc agggcggcgg tcgggcggta tgccggatag
     4981 cgttccactt tcgcttctat attgattttg attggttttc tgacggaatg acccgatgcg
     5041 gcatccggga cggcttgtgt tttttcctgc ccgcctgccg gattttccca tccttgcgtg
     5101 aaaccgaaag agacggcggc gcggcggaca agctcgagat agcgtccttc aagctccggg
     5161 caggcggcgg acacgctttc tgccgtaacc gtgaagccgc cgcccgaccc ggaaaggcgt
     5221 tccgcaatct gcgtgtagat acgccaacgt tgggcgcgca acattgccgc atcgcaaccg
     5281 gcagccgctt tatccaaggc aatcaggccg cgccggtttt ggtggtgaag gcaaaccgtt
     5341 actctggaga ggatatgttg cccgtccaat atttgataca gtttgcgtat cagcagaatc
     5401 aggcggtcaa actcctccat ctccgacagg gaatcaggat ggtcggaagc ggtataaacc
     5461 agtttggaca attttgcggc aaacatattg ttcatcaatc ttccttgtcg gttgaaaccc
     5521 cgctcttcgg ggcggtagaa tcagatttgt ttgggaggga caactcctcc cagatcagga
     5581 cgacacatag gctggtgctt gatgtgttgt ccggcgagtt gaaacattca gccatcctca
     5641 aggtgcggca gttttgccga aacatattct acacggcttc aatgccggac gataaaagga
     5701 aattcatatg aaatggaccg acacccggcg tatcgccgaa gaactctatg acctgcacgg
     5761 cgaaaccatc gatcccagaa ccgtgcgctt tacccaactg cgcgacctga ttatggcatt
     5821 gcccgaattt gatgacgatc ccgcccgttg cggcgaacgt attctcgaag ccgtgcagca
     5881 ggcatggata gacgaggcag aataagtttc gggaatgccg tctgaaatac ggcggtacgc
     5941 ggttcgtgct tctgtttgca gtgggaacgg ttttaccagt ctcctttttt cagcctatcc
     6001 agttggcggc ggtcgcgctt ggtcggtctg ccgtcgggat gggcggaagt gatgcggctg
     6061 aattggtcga gctgtttgcg ctcttccctc aatgctgccg tttttgcgtc ttcttcatac
     6121 agaagccgcg cctcggatgc cgggcggcgt tggtggttca aacctttaac cttgatttta
     6181 tagggaaggg aattgagcgt caggtcgata atatcgccga tgtctatggt tttactgttt
     6241 ttgaccttcg agccgtttac ttgaacccta cccagttcga tgtgcttttg cgcaagggaa
     6301 cgggtcttga aaaaacgtgc cgcccaaagc catttgtcca gccgcatggc ggaagaatcg
     6361 tgcttgtctt tcatacgatt ttgtttgaaa taattgaatt tgtttcgagt ttagcataag
     6421 atacgccgcc tataactagt atatatgcac taatccactg ttttccatgc tgtccgaaca
     6481 caaaaagagg gtgtggaaaa gccgttttgg acaataaatt aactgcggaa tatgcacaaa
     6541 tagcgtatga tagcggcaga atctgttgat gagagcttca ttctatgaaa cctgtttttt
     6601 tggattttga acaacccata gccgaactga ccaacaaaat cgatgagctg cgtttcgtcc
     6661 aagacgagtc tgccgtcgat atttcggacg aaatacaccg tttgcagaaa aaaagcaacg
     6721 acctgaccaa atcgattttc agcaaactca cacccgccca aatttcacag gtttcccggc
     6781 atccgcagcg tccctatact ttggattaca ttgaggcact gtttaccgat tttgaagaac
     6841 tgcacggcga ccgccacttt gccgacgatt atgcgattgt cggcggattg gcgcgtttca
     6901 acggacaaag cgtgatggtc gtcgggcatc agaaagggcg cgacaccaaa gaaaaaatcc
     6961 gccgcaactt cggtatgccc cgtcctgaag gctaccgcaa agccctgcgc ctgatgaaga
     7021 cggcagaaaa attcggcttg cccgtcatga cctttatcga tacgccgggc gcgtatcccg
     7081 gcatcggcgc ggaagaacgc ggacagtcgg aagccatcgg caaaaacctg tacgaactga
     7141 cgcgcctgca cgtccccgtt ttgtgtaccg tcatcggcga agggggctcg ggcggtgcgt
     7201 tggcggtcgc cgtaggcgat tacgtcaata tgctgcaata ttcgacctat tctgttatct
     7261 cccccgaagg ctgcgcttcg attttgtgga aaaccgccga aaaggcggcg gatgcggctc
     7321 aggctttggg cattactgct gaccgcctgc aaaagctgga cttggtcgat accgtcatca
     7381 aagaaccatt gggcggcgcg catcgggatt tcgggcaaac catgaaaaac gtaaaagccg
     7441 ttttggaaaa acaactgcac gaagcgcaaa gcatcccgct tgccgatttg ctttcgcgcc
     7501 gtttcgaccg cattatggct tacggcaaat tttcggaaca ataattcagg tagaacaagc
     7561 agcaagcagt ttgtctgaaa ctgcttgctt tttctttatc gggacggaac cgtgctgact
     7621 ttagatgcgt ttgagcaatg cttgaaggat tgttttcctc aaggtctgaa tggaaaaaaa
     7681 acagcggtgg cattaagcgg cggcttggat tccgtcgttt tgctgcatct gcttgtccgc
     7741 gccgggaaaa aaggcggttt tattccggat gcattgcata tccatcacgg cttgagtccc
     7801 cgtgccgacg attgggcgga tttctgccaa aactattgcg atatgctcgg ggtggggctg
     7861 gaaacggtta agatctgcgt ggaaaaaaac ggtttgggca tcgaggcggc ggcgcggcaa
     7921 aagcgttatg ccgcgtttgc cgaaaaaggc tttgacgttt tggcgttggc gcaccacagg
     7981 gacgatcaaa tcgaaacctt tatgctggcg gtcgcgcgcg gcggcggttt gcgcgctttg
     8041 gcggctatgc ccgccgtccg cccttttggg gaaaaaggca ttatctggcg gcccttgctg
     8101 cctttttcgc gccaagatat atgggattat gcccaaaaac acggtttgcc gaatatcgag
     8161 gacgaaagca atagcgatac ggcttatttg cgaaaccgct tccggcaccg tattttgccc
     8221 gaactttcgg cgcagattcc ccatttcggg cggcatctgt tgaacaatgt ccgtgccttg
     8281 caggaggatt tggctttgtt ggacgaggtc gtcgttcaag actgccgttg ggtttgcgga
     8341 gccggttatt tcgatacggc gcggtggctg acgttttccc cgcgccggaa aacccatatt
     8401 ttgcggcatt ttttgaagga aaacggcatt cccgtgccga atcagaatgc ccttgccgac
     8461 attgcccggg ttttgacgga ggcaaaaacc ggacgctgga acgtgcaagg ctttgaattg
     8521 catcattatg caggcaggct gtttgtgttc cgaccggaaa aaacggataa actgcggttt
     8581 ttgaaagaca ggcagataag cggaaattta agggaaatat tggcggggca gggatttgtt
     8641 ttaaaacgtc atccgttcgg acttcccggg catcttttgg agcaggacgg aatcttgcgg
     8701 acggttgcgg catcggacag gctggctgtc ggcggtatcc ataaggatgt gaagaaaatc
     8761 cttcaggaaa agcgggtttt gcccgcgctg cgcccgtttt ggccggtcgt cgccgacaag
     8821 gacaaccgtc cgctggcgtt ggcaaattac ggtacggatt ttcaaatctc ggtttcagac
     8881 ggcattttgc ccgtccatcc ggattttccc gttttatttt gatggaatcg gaatcgccgt
     8941 tacctattat atattcttga aaaggcggta aagtgaagca tatcagttcg gcaaacaatg
     9001 aacacatcag acacctgcac cgcctgttgt ctcaagggaa gttcagacgg caatacgccc
     9061 aaaccgtttt ggagggcgtg cacctgcttc aggttttcct gcaatccggc gggatgccgg
     9121 tcggggtata tattcccgaa gcgaaaatgc cgtctgaaga agtccgtaaa ttgacggcgg
     9181 ttttgccgga agacggggtt ttttccgttt cagacggcat attgaagaaa atcagcagcc
     9241 tgacttgcgc ggatgatgtg cttgcgctga ttgatattcc ggatgcgggt gctttgccgg
     9301 ccggcggcga ctgtgtggtt ttggacggcg tgcaagatcc gggcaatgtc ggcacggtgt
     9361 tgcggagcgc ggcggcggca ggaatcggcg cggtcgtttt gggcaaaggt tgtgcggacg
     9421 cgtggtcgcc caaagtgctg cgagccggaa tgggcgcgca tttcttgtcg gagatttatc
     9481 cgcaggcgga tttggaaata tggttggcgc gttatgaaga ccgtgtgttt gccaccgcgt
     9541 tgcgtgagga aaaacaggcg gttctgtata acgaagattt gcgcgaaccg acagcctggg
     9601 tgtttggcaa cgaaggcgcg ggtgtcggca aagcggtttt agacagggcg gacaagtgcg
     9661 tcaggatacc gatgcacgat gcaaccgagt ctttaaatgt cgcgatggcg gcgacaatct
     9721 gcctgtttga acagatgcgg cagcggtcgg cgtattgagg aaaagaaatg ccgtctgaaa
     9781 aaatctatta cggcgtattg attttcttat gtatcgcttc tatgctgctg tcgccgtttt
     9841 tttatgcggg tgctttgaag cccaagaagg cggcattgcg gaaggacggg cagtggaaac
     9901 tcatcttatt gtccaatacc gtggcggcgg cggttttggc ttgggtgtgg tggaaatggt
     9961 tttgacagat attgctcaaa atcgtgctaa tggaatccga acaaataaag aattttgtaa
    10021 aaaatttgtt aaatcaacga attaaagttt tgtggaaaac aaaacagctc taagcaaata
    10081 gggcgtttgt cggtaaatac ggaagagttg cggcattatc gggcatcttt aacaagtagt
    10141 gccgtcttgg caggcaatcg gtttttatgg gcagcttgca aaatcgcgga tataaaattg
    10201 cgaatcggtt aaagtgtggg gacgctatga aaaattgcga attttttatg acccgacaag
    10261 ggcaatctat gatagcggtg cagattattt aaccagagaa aaacatagat tcgtcgtaat
    10321 tgcaaatagt gcttgggggc tattgcttaa tttatcttgt tattatgaca aggttttgga
    10381 aaagcggaaa ataccgttcg gcaaacagga aattgatgac gatatggaca aagtgtccgc
    10441 ccataagcgg aagtttaaag atatttctga aatcaaagta ggggatggtt gggaataccc
    10501 gttcaattat gagcagggaa tgaaagaatt agatgaagtg ctattgaaat acattccctt
    10561 ttttgaagaa gaacgataaa ggaggttgat atgcgcgtat ctaaaataat tggaagtatg
    10621 ttgcttgtta cagcggttca gaccgtattt tcggcaaatg tttacgcgtg ccgccataat
    10681 ggtaaaacca gttacagcca aactccggga aaacattgta ccaacgcggg tttggggcgg
    10741 gatcgggtgt acagttcggt tagaccggca gtaaaagaca gggcggaaga cgcaggggtc
    10801 ggcgattatt cggacacggt gagggacgaa cacgtccaaa atccgagaga gaatacgcat
    10861 aaggatgctt cgaataccgg tgcaaagacg cattgattga aaccgaatca gccatgacgg
    10921 cgttgaataa tagaatttga acgattaggg aactctgatg aaacacatcc acattatcgg
    10981 tatcggcggc acgtttatgg gtgggattgc cgccattgcc aaagaagcag ggtttgaagt
    11041 cagcggttgc gatgcgaaga tgtatccgcc gatgagcacc cagctcgaag ccttgggcat
    11101 aggcgtacac gaaggcttcg acgcggcgca gttggaggaa tttaaagccg acgtttacgt
    11161 catcggcaat gtcgccaagc gcgggatgga tgtggttgag gcgattttga accgcgggct
    11221 gccttatatt tccggtccgc aatggctggc tgaaaacgtg ctgcaccatc attgggtact
    11281 cggtgtggcg gggacgcacg gcaaaacgac caccgcgtcc atgctcgcat gggtcttgga
    11341 atatgccgga ctcgcaccgg gcttccttat cggcggcgta ccggaaaact tcagcgtttc
    11401 cgcccgcctg ccgcaaacgc cgcgccaaga cccgaacagc caatcgccgt ttttcgtcat
    11461 tgaagccgac gaatacgaca ccgcgttttt cgacaaacgc tccaaattcg tgcattaccg
    11521 tccgcgtacc gccgtgttga acaatctgga attcgaccac gccgacatct tcgccgattt
    11581 gggcgcgata cagacccagt tccaccacct cgtgcgtacc gtgccgtctg aaggcctcat
    11641 cgtctgcaac ggacggcagc aaagcctgca agacactttg gacaaaggct gctggacgcc
    11701 ggtggaaaaa ttcggcacgg aacacggctg gcaggccggc gaagccaatg ccgatggctc
    11761 gttcgacgtg ttgcttgacg gcaaaaaagc cggacacgtc gcttggagtt tgatgggcgg
    11821 acacaaccgc atgaacgcgc tcgccgtcat cgccgccgcg cgtcatgccg gagtcgacat
    11881 tcagacggcc tgcgaagcct tgagcacgtt taaaaacgtc aaacgccgca tggaaatcaa
    11941 aggcacggta aacggcatca ctgtttacga cgacttcgcc caccatccga ccgccatcga
    12001 aaccaccata gaaggtctgc gtcagcgcgt cggaaacgcc cgcatcctcg ccgtcctcga
    12061 gccgcgttcc aacacgatga agctgggcac gatgaagtcc gccctgcccg aaagcctcaa
    12121 agaagccgac caagtgttct gctacgccgg cggtgtggac tggaacgtcg ccgaagccct
    12181 cgcacctttg ggcggcaggc tgcacgtcgg caaggatttc gatgccttcg ttgccgaaat
    12241 tgtgaaaaac gcccgaaccg gcgaccatat tttggtgatg agcaacggcg gtttcggcgg
    12301 aatacacacc aaactgctgg acgctttgag atagcccggg cgatgccgtc tgaaagccct
    12361 tcagacggca tcgtccggct gcgcggcaca aaggcggaaa aaccgtttgc cccgtatttt
    12421 caaacgcgtt acacttgccg ccgctgtttt cagccatttg attacccgca accgccgtca
    12481 ttgcgccggc ggtttgcctg tcagcgtcat tgcgccgctg taaatacgaa agaacacatt
    12541 atgaccgtat cccccgtcgc cttgcgccgt aagaccgagt gcaagcctca tcccaccgcg
    12601 cgctattgga aaaaatgcga tgtcgaagcc ctgttcggac ttcccttcct cgacctcatt
    12661 taccaagccg ccgaaatcca ccgccaaaat ttcaacccgc gcgaaatcca gctttccacg
    12721 ctgttgtcca tcaaaaccgg cggctgtccc gaagattgcg cctactgtcc gcaatcggcg
    12781 caccacaaca ccaatctggg caaagaacag atgatggatg tggatgaaat cgtcgaaaaa
    12841 gccaaaatcg ccaaatcgcg cggcgcaagc cggttttgca tgggcgcggc gtggcgcggc
    12901 cccaaaccca aagacgtgga gacggtttcc gcaatcatca aagccgtcaa gggcttgggt
    12961 atggaaacct gcggcacgtt cggtatgctc gaagaaggta tggcggaaga cttgaaagag
    13021 gcgggcttgg attattacaa ccacaacctc gacaccgacc ccgaccgcta caacgacatc
    13081 atccatacac gccggcatga agaccgtatg gacaccttgg gcaaagtccg caacgccggt
    13141 ttgaaagtct gctgcggcgg catcgtcggg atgaacgaaa cccgcgccga acgcgcaggg
    13201 ctgattgcca gcctcgccaa cctcgacccg cagcccgaaa gcgtgccgat taaccggttg
    13261 gtcaaagtgg aaggtacgcc gcttgccgat gccgaggatt tggactggac ggaatttgtc
    13321 cgcaccatcg ccgtggcgcg gattacgatg ccgcaaagtt atgtccgact gtcggcaggg
    13381 cgcagcaata tgccggaagc gatgcaggcg atgtgcttta tggcgggcgc gaactcgatt
    13441 ttttacggcg acaagctgct gaccacgggc aatccggatg aagacggcga cagaatcctg
    13501 atggaaaagc tcaacctgta tcccttacag tttgagctgg aaggcgaggt cgccgaagtg
    13561 gaaaaagcct ctgggattaa agtggattat tgacgattgc aaaaatgccg tctgaaaccc
    13621 gaaaaaaggc tttcagacga catttgttcg gacggtattt ccaatatctt tttaccggcg
    13681 cgtgatgttg ccgtcgggcg agacatccag cccgttctcg ttggggaagt cgctgcggtt
    13741 cagataaata atccgcccga taacggtttt tcagggtcga ttttggggat tttatcgtcg
    13801 gcttgggtaa tagggatgat agttccggaa ataaatttac cctgtttgtt ggtgataatt
    13861 ttgaaaatcg gcgcaatgcc gctgatgccg gaggtgttga ttgcgccgta ggtggcaaag
    13921 ttgccgccgc tgtaagagat gaagcggtcg cggtaaagtt cgacggcgcg tgtaacgtgc
    13981 ggcccctgcc cgaatacgac atccgcgccg gaatcgacgg caagccgtgc aaactcgacg
    14041 acgttgcccc tgttttcccc atagaagatt tcggtatcga acggcaggtg ttccgcctgt
    14101 ttcccttccg cgccgccgtg gaacattaca atgacgatgt cggctttctg tttggttttg
    14161 gtaatccgtt ttctaacttt ggcataatcg ttcagtttga cggcggcaag gttgggggcg
    14221 aaggagacga agccggatct tacgccgttt ttcttcagga tggcggtttc aaacctgttt
    14281 tcgatgcccg aatatttgat gttcaattcg tcaaggttcg ccctttatgc cgtttccgca
    14341 ccgcaaaccg ccggggtcaa gccctcggcg catcctgccg gaacggaatc cccgtgctgc
    14401 cgattgacgg ttcaaacccc gcgcccgttt caaataccgg cgatgtggac ggacaggatg
    14461 cgcctgacga aaagacagcc gataccgttt ccattatcgg cgtgggcgac attatgctcg
    14521 gcagcaatta tccggtcgat tacctgcccg ataccaatat tctgaaaaac gtcgaatctg
    14581 ccttgcaaga cgcggacatt accgtcggca acctcgaagg cacgctgttt gacgaaggcg
    14641 gtacgccgaa aaaatgtgca accccccaaa atatgctatg cattccgaac gccctccgca
    14701 tacgggcaat accttgccga cgcgggattc gactacctca gcttcgccaa caaccacagc
    14761 aacgacttcg gcgcgcaagg catcacggca acggcggcga gcggcagctc ttttacatac
    14821 tcgatcgcgc taaagccgct gccgataacg aggccaaaat tgcggaaaat accgccatcg
    14881 cccagataaa tttgtccatc atcagacctt tactgttcag acgagacagc atttgccgca
    14941 cgttttgggg cttatctttc gatttgcgct atgtcgcgca ccgcgccttt gtcggcggaa
    15001 gtcgccatcg cgccgtaagc tcttaatgcg gcggagacgt agcggtcgcg gtttttaggc
    15061 ttccatgctt tgctgccgcg cgcttccatt tcggcacggc gtgcggcaag ctcttcatcg
    15121 gaaatggcaa ggtggatgct gcggttgggg atgtcgattt cgacggtatc gccttcgtgt
    15181 accaaaccga tcgcgccacc ttccgccgct tcgggcgagg cgtgtccgat ggacaaacct
    15241 gatgtgccgc cggagaagcg tccgtcggtt aagagagcgc aggctttgcc gaggccttta
    15301 gatttcaggt aggaagtcgg atacagcatt tcctgcatgc ccgggccgcc tttcgggcct
    15361 tcgtagcgga tgatgacgat gtcgccggcg acgatttggt tgcccaaaat gccttctact
    15421 gcgtcttctt ggctttcaaa cacgcgggcg cggccggtga atttgaggat gctctcgtcc
    15481 acgcctgcgg tttttaccac gcagccgcgc tcggcgatgt tgccgaacaa gaccgccaaa
    15541 ccgccgtctt gcgagtaggc gtgtgccacg tcgcggatac agcctttttc gcggtcgagg
    15601 tcgagggttt tccacatacg gttttgcgag aacgcttggg tggtgcgtac gccgccgggc
    15661 gcggctttga agcgttcgat ggcgcgggtg ttttcagggt tggttacgtc ccatttttca
    15721 atcgcgtctt tcagcgtcgg tgcgtggata gtgtgcacgt cggtgtgcag cttgcccgct
    15781 ttgtccaatt ctttcaggat ggcgaaaatg ccgccggcgc ggtgcacgtc ttccatatag
    15841 tagtcgtggt tgttgggtgc ggttttgcag atgcagggca caacgcggct taagcggtca
    15901 atgtcggcca ttttgaaatc gacaccggct tcgttggcaa cggccaacag gtgcaaaatg
    15961 gtattggtgc tgccgcccat cgcaatatcc atcgtcatag cgttttcaaa cgcttttttg
    16021 gtggcaatgc tgtgcggcag cacggtttcg tcgttttgtt cgtaataacg tttggtgatt
    16081 tcgacaatca tacgtcctgc ttcgaggaac aattctttac ggcctgcgtg ggtcgccaaa
    16141 tacgaaccgt tgccgggtag ggacagaccg agtgcttcgg tcaggcagtt catcgaattt
    16201 gccgtaaaca tacccgaaca cgaaccgcag gtcgggcagg cgttttgttc gacttcctcg
    16261 acttgccggt tgctgacatt gtcgtccgcc gattcgatca tcgcgtcaat caagtccaaa
    16321 cggcgttcgg gttggatgtt tgccacgccg ataactttgc ccgcttccat cgggccaccg
    16381 gaaacgaaga tggtcgggat attcaggcgc atggcggcaa tcagcattcc cggggtgatt
    16441 ttgtcgcagt tggaaatgca caccagcgcg tcggcgcagt gggcgttgac catgtattcg
    16501 atggagtcgg caatcaaatc gcggctgggc agggaataca gcatgccgct gtgtcccata
    16561 gcgatgccgt cgtcgatggc gatggtgttg aattctttgg cgatggcgcc ggctttttcg
    16621 atttcgcggg caaccagctg gcccatattg tgcaggtgga catgacctgg cacgaattgg
    16681 gtgaacgagt tggcaacggc gataatcggt ttgccgaagt cggtttccat cacgccggtg
    16741 gcgcgccaca atgcgcgcgc gcccgccata ttgcgaccgt gggtggaggt tttggagcgg
    16801 tagtccggca tagtgtgttt ccttgtctgc tatgccgtct gaacggcaaa aggctttcag
    16861 acggcatttt tgttgtgttt tgaaatgaaa aaaaaggtat tttataccaa gtatcggaat
    16921 tttgcgggat tgaaacggcg tgcggcaaaa aagaaaatcc ccgcaggaat gcggggacgg
    16981 gttcaggcgc gggcaatcgc gacggctttg gatgcgtccc aaaaatcaac gggggcattt
    17041 aatacgggtt tgacgatgcc cgtccgtatg gcgaaatcgc cgaaaccttc gccgatattg
    17101 cgttctgccg cccatttgcc gatcaggtcg tccaattcgg caaggatttc gggcagggtg
    17161 atgttttctt tgtaaaggcg ggggatgcgt acgccttcac ggtcgccgcc gatatggagg
    17221 ttgtagcgtc cgacggcttt gccgaccagc ccgatttccg ccaacattgc ccgtccgcag
    17281 ccgttggggc agccggtaat gcgggtaacg atatagtcgt ccgacgtgcc gtgtttcgcc
    17341 ataatcttat ccagatcgtc gatgaagtct ggcagcacgc gttcggcttc cgccattgcc
    17401 aaggggcagg tcggaaagga aacgcaggac atcgcatttt cgcgcagctt gctgacatcg
    17461 ttgcggatta atccgtatgt tcgggcaagt tcttcgattt ttgctttgtc tgcttcggca
    17521 acatttgcca cgatgaggtt ttggtttgcg gtaatgcgga aatcgccttt gtggattttg
    17581 gcgatttcca acacgccggt cagaagctgt ttcccgcctt cgtcaaccaa acgcccgctt
    17641 tcgatgaaaa gggtcaaatg ccagttgccg tctatgcctt tcacccagcc gatgcggtcg
    17701 ccgcgcccgg taaatttgaa cgggcgtatg ggttcgaacg gcatacccat acggcgttcg
    17761 acttccgcgc ggaagttgtc caagcccata ttttgaatgg tgtagcgggt gcgggcgttt
    17821 ttgcggtcgc tgcggttgcc gaagtcgcgc tgtgtggtta ccaccgcttc ggcggccttc
    17881 agcgcgtgtt ccggaggtac gaaacccagt tccagtgaaa tgttcggata ggttttggtg
    17941 ttgccgtgtt ccattgaaag cccaccgcct gccaaaacat taaaaccggc aagctgtccg
    18001 ccgccgtctg aaatggcgac aaaatccaag tcgttgccgt agcagtccac atcatttaag
    18061 ggcgggatga cgactgcggt tttgaatttg cgcggcagat aggttttgcc caaaatcggc
    18121 tcgtcttctt gaaggaagtc gtcggaactt tgaacttttt tgccgtccac ccaaacatcc
    18181 agataaccgc gcgtgcgcgg cagcaagtgt tcggaaatct ttttcgcgta ttcgtaagcc
    18241 tgttggtgga gtgccgactc gatgggattg gaggtgcaaa gcacgttgcg gttcatatcc
    18301 gccgccgtgg cgatggaatc caaacccagt ttgtgcaaga ggcggtgcat cgtctgcaac
    18361 ttggttttcg gcacgccgtg aaattggaag gtttgccggt tggtcagcct gatggatcgg
    18421 taataactgt tttcgcgggc gaatttgtcc aattctatcc attgggacgg cttgatgatc
    18481 ccacccggca gccggcagcg caaaagcata aatttcaagg gttcgagttt tgcctcggcg
    18541 cgttcggcgc ggatatcgcg gtcatcctgc tcatacatgc cgtgg
//
LOCUS       DAWHXZ010000014        17987 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000014 DAWHXZ010000000
VERSION     DAWHXZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17987
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.014"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            1..203
                     /locus_tag="WIE35_000325"
                     /pseudo
     CDS             1..203
                     /locus_tag="WIE35_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(680..1051)
                     /locus_tag="WIE35_000326"
     CDS             complement(680..1051)
                     /locus_tag="WIE35_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153303.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITPEGLITKNSNPK
                     LFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLLNDDAYIEFAYSEDFYVLPEIKIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(1185..1628)
                     /locus_tag="WIE35_000327"
     CDS             complement(1185..1628)
                     /locus_tag="WIE35_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153304.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1671..2540)
                     /locus_tag="WIE35_000328"
     CDS             complement(1671..2540)
                     /locus_tag="WIE35_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ4153305.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNSKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLINPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            2704..3627
                     /locus_tag="WIE35_000329"
     CDS             2704..3627
                     /locus_tag="WIE35_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ4153306.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALALTRTDLPYPLDVVSRLVRGEKFFTTQEVEGV
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(3705..4346)
                     /locus_tag="WIE35_000330"
     CDS             complement(3705..4346)
                     /locus_tag="WIE35_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ4153307.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVSARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGNSEGTHDYGHGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(4401..5192)
                     /locus_tag="WIE35_000331"
     CDS             complement(4401..5192)
                     /locus_tag="WIE35_000331"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ4153308.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFESEKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFYL"
     gene            5358..5433
                     /locus_tag="WIE35_000332"
     tRNA            5358..5433
                     /locus_tag="WIE35_000332"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5391..5393,aa:Thr,seq:tgt)
     gene            5575..6366
                     /gene="panB"
                     /locus_tag="WIE35_000333"
     CDS             5575..6366
                     /gene="panB"
                     /locus_tag="WIE35_000333"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ4153309.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTESVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTVKFVKNFMQGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            6356..6532
                     /locus_tag="WIE35_000334"
     CDS             6356..6532
                     /locus_tag="WIE35_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153310.1"
                     /translation="MQTEQGLLPMPSESRFRRHFVLPCAARIIGAFCRAGSPKDKDYR
                     YANHTYHSRTARVA"
     gene            6489..7325
                     /locus_tag="WIE35_000335"
     CDS             6489..7325
                     /locus_tag="WIE35_000335"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ4153311.1"
                     /translation="MQIIHTIQELRAWRKNAGTVAFVPTMGNLHEGHLALVREAKKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFLGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEICRVDTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            7490..9328
                     /locus_tag="WIE35_000336"
     CDS             7490..9328
                     /locus_tag="WIE35_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153312.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPVGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            9468..10049
                     /locus_tag="WIE35_000337"
     CDS             9468..10049
                     /locus_tag="WIE35_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ4153313.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            10059..10904
                     /gene="ispE"
                     /locus_tag="WIE35_000338"
     CDS             10059..10904
                     /gene="ispE"
                     /locus_tag="WIE35_000338"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ4153314.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCSRTTQQLIDLGATLGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRDSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            10912..10987
                     /locus_tag="WIE35_000339"
     tRNA            10912..10987
                     /locus_tag="WIE35_000339"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10945..10947,aa:Gln,seq:ttg)
     gene            11000..11075
                     /locus_tag="WIE35_000340"
     tRNA            11000..11075
                     /locus_tag="WIE35_000340"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11033..11035,aa:Gln,seq:ttg)
     gene            11084..11159
                     /locus_tag="WIE35_000341"
     tRNA            11084..11159
                     /locus_tag="WIE35_000341"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11117..11119,aa:Gln,seq:ttg)
     gene            11327..12310
                     /locus_tag="WIE35_000342"
     CDS             11327..12310
                     /locus_tag="WIE35_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ4153315.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            12377..12949
                     /locus_tag="WIE35_000343"
     CDS             12377..12949
                     /locus_tag="WIE35_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ4153316.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTTVEVLALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(13074..14243)
                     /locus_tag="WIE35_000344"
     CDS             complement(13074..14243)
                     /locus_tag="WIE35_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ4153317.1"
                     /translation="MTAHKILPVLLSIILGISHATAASPVPNRPTAHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTITEKEIVALENVEKRSRWQRLWMRLTGQ"
     gene            14391..15917
                     /gene="ilvA"
                     /locus_tag="WIE35_000345"
     CDS             14391..15917
                     /gene="ilvA"
                     /locus_tag="WIE35_000345"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ4153318.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(15974..17047)
                     /locus_tag="WIE35_000346"
     CDS             complement(15974..17047)
                     /locus_tag="WIE35_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ4153319.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKEQICTKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(17044..17904)
                     /gene="cysW"
                     /locus_tag="WIE35_000347"
     CDS             complement(17044..17904)
                     /gene="cysW"
                     /locus_tag="WIE35_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ4153320.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
BASE COUNT         4268 a         4092 c         4999 g         4628 t
ORIGIN      
        1 acacattttg ccgaacgaca aaaccatatt aatggaattg agaacttttg gaaccaggca
       61 aaacgtcatt tacgcaagtt taacggcatt cccaaagcgc attttgagct gtatttaaag
      121 gagtgcgaat ggcgttttaa caacagtgag ataaaagttc ttgttccatt ttaaaacaat
      181 tagtaaaatc aagtttatcc tagttatcta ggacagcccc taaacttttt ggaagcatct
      241 ctaatttatt aatatcgttc atatccccta cacacagaaa aaatcttggg gaatttcctt
      301 taatcttttt aataaatctt cacaaagcct aaaatgattt aattttagtt tctttttatt
      361 tttttgataa attctagata acctcgctta ttaggaaaaa tttcaggata tattcctttt
      421 tcttctaaag aagtatagtg gattaaattt aaatcaggac aaggcgagga agccgcagac
      481 agtacaaata gtacggcaag gcgaggcaac gccgtactaa tccactatat atatccattc
      541 atatagagaa aaattcttat acccaatata aaacaaggtc tttcattata ttttgaaaaa
      601 cctagaagct ttgtatctat tggtttatca aaattaataa ttattgcaga agacatgtag
      661 gctaataatc ttgatttatc tatattttat taattatata tttaaccccg aagtcaatat
      721 agaaatttag tgattttaaa gttctcttat caattttaat ctcagggagt acataaaaat
      781 cctcagaata ggcaaactct atatatgcat catcattcaa taataattca ataatatttt
      841 tcatttcatc cgaaagaaat gtaaccatct catattgaca tttattttta gataccattt
      901 ttgaatattg attaaataat tttggattac tatttttagt aattaatcct tccggagtaa
      961 taaaaccact ttgtataaag tcatcactta tattaacagc cttccgaaaa aaaatataat
     1021 cacactcttt gttatcaata taaaatctca tactctttaa taatgaaata taagaatttc
     1081 cagaattaca agcaatttga aaaaacatca ttactcctaa aactcataaa tagtaaggaa
     1141 tatatatata tgcatattcc ttactatttt ttcacaaatt atgcttatct atgcttattt
     1201 ccataaaaag catgccctcg aattttttga cttgagttct ggaaatgaga ataatttgaa
     1261 tggatttcct tctgtccatg tctaataaca ttttgtcttc cccaagcttg gtttgctacc
     1321 gctcttgctt gttgagatgt tcgtgcatag acattagctc ctcttgataa agcttgtgct
     1381 gcaacacgct gcgtaaccca acgctgcgca aggaatctgc ccgcatgcaa aattcctatt
     1441 gccaccaacg gtgccgcagc cccttcagtt tctttcatct ccttttgaga aagctccgcc
     1501 aactgcatcg gcgcatctgc ctgcgtgtgg aacacttggt tttcaaatgt ctgattgtcc
     1561 aagccgtttg ccattgcggg ggcaatcata gacagcatca ttacggctgc ggtgatttgt
     1621 tttttcataa taactccttt ggattacaag gttggaaaat caaaacctgc ttaaaatgta
     1681 tgctgtacgc caaatttcag ttcggaactg ctttgccctg aaacgttgaa acgtgcggat
     1741 gcgtttaaag ccgtggtttt ggtgaaaccg aaacctgtgc cgaaatgagc gtaggtggat
     1801 gtgtttcttg cggtttcttt tttgccgtct atgcggtcgg gctgtgtgcc cagccattgg
     1861 atgcctccgg tcaggctgat tctgtcgttg gcggcaaatg agatgttggg gttgatcagc
     1921 aggtagttgc ccgatttgta gcggatgccg tctgaaaggg ttttgctgcc gttgatacgg
     1981 taggcggcgg tgagggaaag gacaatcgga tctatggctt tgtaggtggt ggcgccgatg
     2041 agccaggatt ttcccgacga ggctttgttg cgcgattttt cgtaaaccgt gctttcaaga
     2101 aagctgatta gggcggggtt tttgtcgtct ttaaggaaag tgtggctgat gccgagggat
     2161 acgtcggaca tccgtttgtt gcgggttttg ctgttgccgt cgagtttgcg ttcttcgtgc
     2221 cacagatagc tgccgctgcc gtaaatgtcg gtattcccgg tcagtccgta gcgcaaaccg
     2281 agcgtgccaa cgagcatatc ggtattgctg ccgttttctt ggatttcggt cggaatgggg
     2341 ataaacgagg ttgcgccggt ttggatgtaa accggtgcgg caagttcggc gcggttgttt
     2401 tcgctattca ggtaggtaag ggaagtttcc agtttccatt tgcccttgtc agtcattatg
     2461 tcttcaatcg tcaagggcag gtcggcataa gcggataaag gcaggatggc gggcaaggcg
     2521 ggcaaaaaga tgcgcttcat atttctccta ttgatattaa ttcttattta ctgataataa
     2581 tgaatatccg ataatcttgt ctaaataaat ttttaaatca taaaaaacaa tatgtttgtt
     2641 tttgtcaatt atgtttcgat atgccgtctg aaaagtttgt gaaaaacggt tacaatccgc
     2701 cgcatgaaaa aacgcaataa tcctcttccg ctgttgaacg gtgtcaaacc cagttatttg
     2761 gtgctgccgc atgaaaagca gttttacggg ctgccgctgc tgcattttct gtgcatccgc
     2821 tttccttttg tgggcgcgga cgattggcgc aggcggttga acagcggttt tgtggtcggt
     2881 tcggatggtg cggcgttgga cgaacatact ttgttcgagc cgggtaaggt gatgttttat
     2941 tatcgggaaa ccagcagtga ggatgagccg cgtattccgt ttgaagaaaa gattttgcat
     3001 attgatgagc atttgattgt ggtggacaag ccgcattttc tgcccgtcat ccccagcggg
     3061 cgttttttaa gggaaacgct gctcacacgt ctgcgcctgc gtcccgaatt gcagcatttg
     3121 aatgttgaag acattacgcc gctgcaccgc ttggacaagg atacggcagg cgtgatgttg
     3181 ttgtcgcaca atcctgccac gcgcggggct tatcagacga tgtttcaaaa caaaacggta
     3241 tggaaaacgt atgaggcgct tgcgctgaca aggacggatt tgccgtatcc gctcgatgtg
     3301 gtttcgcgtt tggtaagggg tgaaaaattt tttacaacgc aagaggtgga aggcgtgcca
     3361 aacgcacata cgacggtcga actgattgaa aacagggggg aattcagcct ttaccgcctt
     3421 acgccgcata ccggcaagaa acaccagttg cgcgtgcata tgatgggctt gggtatgccg
     3481 ctgttgaatg acgcgctcta tcccgtgccg tctgaagcgg gcagcgagga ttatcggaaa
     3541 cctttgaagc tattggcaaa aaagattgcg tttgcagatc ctttgtcggg tcgggaaagg
     3601 gtgttttgca gcggattttg cctataatga gagggaacgc aaacccgtat cggcggatgc
     3661 caccgatacg ggttttacat tatcgatgcc gtctgaaagg ggctttatcc gttcaggcat
     3721 acgggcgcga agcggaggat ggtgtcgcgc aagacgatga gtttgccgtg aaagaaatgc
     3781 gtggaaccgg cgatggtaat gacgggcaaa tcttgcggtt ccgcccattt cagggctttc
     3841 ccgatttcga cgacttcgtc ttccgcaccg tgtatcatca gggttttggc aacgttggga
     3901 acggcggacg gttccggacg gtcggtatag tggcagactg ccgcgccgat gagcagtaac
     3961 aggtcgggta tgcgcgcttg tgcggcaaat gtggcgacat agccgccgaa ggagaagccg
     4021 gataaggcaa attcgggggc ttcgggatgt tgggcgcggg catagtcgat gacggcgagg
     4081 cagtcttgcg tttcgccgtg tccgtaatcg tgtgtgcctt cgctattgcc tacgccgcga
     4141 aggttgggca ggtagcagtg gaagccgagt ttgcttaagg ctttggcggc agtttggatg
     4201 actttgttgg tgtttgtccc gccttgtagg gggttgggat gattgatgac ggcaacaccg
     4261 cgtgccgata cttgttcgga cgggatatgg atggtttcta aaatgccggc aggaccggat
     4321 atgtgtatgg tttcgggttt cagcataatg atttcctctt gattttccgc atgttccaat
     4381 gcggtcatga agatagttgc ttaaaggtag aaatgcttgc cgttgttggg gttggacgct
     4441 ttcaggccgg caagcatgcg gtggaagtcc aatccgtatg cgttgctcag tttgtcggca
     4501 cgttttttga gtttgtctat gttttgctct ttggggctgc tttggaaggc cattaccgcg
     4561 acattgccgt ggctttcggc aggaagttcc aatacgcgcc cttcaaaaac gctcaacaac
     4621 cgttcgatga agcgttgata gcgtttgtcg ccactccacc agttggtaac gaatatgccg
     4681 tctgaagaga gtgcgtttcg gcagtctcgg aagaacggtt cttcaaccag cgtatcgatg
     4741 atttgttcgc cgtcgaatcc gtccaccaag atgatatcag tgttgtggcg gaagactttg
     4801 atgtactctg caccatccgc ttcaataatt tcaaatttct cactctcgaa aggcaactca
     4861 aacaggttgc gggcaatggc gataacctgc gggttgatat ctacggcagt ttgacgcgtg
     4921 tcgggcaggt aggtgtctat ccagcgcgca aacgagccgc cgcccaagcc gatttgggtg
     4981 atatgctgcg gaagcgcgtc ggtaaacagc agccatccca tcatcgcgcg gctgtaagag
     5041 agtaccagct cggacgggtg gtcgaggttc attgagcttt gaacggtgtc gctgcccaag
     5101 tgaagcgaac ggatattgcc ttcttcggaa atgccgactt cgggaaagcc ggattttgca
     5161 ggacgcaggc ggcggtaggg atgtcttgcc atcggtgtga acggtcggtt tgaaaggcgg
     5221 atattttatc agaatgccga tgcttgtcct gcttcaaatt aatttctttt caaataaatt
     5281 acttattcgg atttgccggg gctttcggat aaattccttg ccaaagtgtg aagttgcctg
     5341 cataattcgc cttctttgcc ggtatagctc agttggtaga gcacctgact tgtaatcagg
     5401 gggtcccgag ttcgactctt ggtgccggca ccagatttga cagtccgatt gtgtaaaaca
     5461 gtcgggctgt tttgcatttg cggcacggtc ggtggcaggg ctgcgggcat ttcactataa
     5521 tagcccgttt caaactgaaa aggccgtctg aaaagggcgg ggtaacaata tctgatgatt
     5581 actgtgaaca cactgcaaaa aatgaaggcg gcgggcgaga aaatcgctat gctgaccgct
     5641 tacgaatcca gttttgccgc gctgatggac gatgccggcg tggatgtgct gctggttggg
     5701 gattctttgg gaatggcggt tcaagggcgg cagtcgacgc tgcccgtcag cctgcgcgat
     5761 atgtgttatc acaccgaatg tgtagcacgc ggtgcaaaaa atgcgatgat tgtcagcgat
     5821 ttgccgtttg gtgcatatca gcagagtaag gagcaggcgt ttgccgccgc cgccgaactg
     5881 atggctgccg gcgcgcatat ggtcaaactc gaaggcggtg tgtggatggc ggaaacgacc
     5941 gaatttctgc aaatgcgcgg tattccggtc tgtgcccaca tcggtctgac cccgcaatcc
     6001 gtgtttgcct tcggcggata caaggttcag gggcgcggcg gcaaggcgca ggcgttgctt
     6061 aacgatgcca aggcgcatga tgatgcgggg gcggcggtcg tgctgatgga gtgtgttcct
     6121 gcggaactgg caaaaaaggt aactgaatcc gtttcctgtc cgaccatcgg catcggcgcg
     6181 ggtgtcgatt gcgacgggca ggttttggtg atgcacgata tgctcggcat tttcccgggt
     6241 aagacggtga aattcgtcaa aaactttatg caggggcaaa gcagcattca ggcggcggtt
     6301 cgggcgtatg ttgccgaagt caaagccaaa accttccctg ctgcggaaca tatttttgca
     6361 gactgaacaa ggtttgctgc cgatgccgtc tgaaagccgt ttcagacggc attttgtttt
     6421 gccgtgcgcg gcgcgtataa tcggcgcgtt ttgtcgggca ggaagcccga aggataagga
     6481 ttaccgttat gcaaatcata cataccattc aagaactgcg cgcgtggcgt aaaaatgcgg
     6541 gaacggtggc atttgtgccg accatgggca atctgcacga agggcatctt gccctcgttc
     6601 gcgaagccaa aaaacgcgcc gacaacgttg ttgtcagcat attcgtcaac cgcctgcaat
     6661 tcggtcaggg cgaggatttc gacaaatatc cgcgtacttt gcaacaggat gcggacaagc
     6721 tggaagccga gggtgttgcc gttgttttcg cgcccgatga gaaagaactc tatccgaacg
     6781 tggaacagcg ttacaacgtc gaaccgccca atctgcaaaa tgagttgtgc ggcaagtttc
     6841 gtccgggaca tttcctcggg gtggcgacgg tggtgtccaa gctgttcaat attgtcgcgc
     6901 cggatgttgc ctgtttcggc aaaaaagact atcagcagct tgccgtgatt aaaggtttga
     6961 ccgaggattt gaattttgat gttgaaatag tgcctgtcga tacggggcgc gcggaagacg
     7021 gtttggcact ctccagccgc aaccagtatt tgagtgcggc ggaacgcgac gaagcaccgc
     7081 gcctgtaccg cgaattaaag gctgttgccg aatccttggc gcagggcagt ttggattatg
     7141 caggtttgga aaaacgtgcc gtccaatccc tgacagaata cggctgggtg gtcgattatg
     7201 tcgaaatctg ccgcgtcgat acgctcgaag tggcgcgggc gggagataag aaactggtgg
     7261 tcttggccgc cgcccgtctg gggacgacgc gcctgattga caatttggaa ataaaactcc
     7321 cttaaaccgc aagcgtcggg aatgccgtct gaagcggatt tgcgtttcag acggcattta
     7381 tttttttgaa cggggtttcg caaatctaca aacgattctg cttgtgataa agttacgcct
     7441 gattatatgc cgtctgaaag ttcggacggc tgtcggataa aggaagatta tgttacccgc
     7501 ccgtttcacc attttatctg tgctcgcggc agccctgctt gccgggcagg cgtatgccgc
     7561 cggcgcggcg gatgcgaagc cgccgaagga agtcggaaag gttttcagaa agcagcagcg
     7621 ttacagcgag gaagaaatca aaaacgaacg cgcacggctt gcggcagtgg gcgagcgggt
     7681 taatcagata tttacgttgc tgggagggga aaccgccttg caaaaggggc aggcgggaac
     7741 ggctctggca acctatatgc tgatgttgga acgcacaaaa tcccccgaag tcgccgaacg
     7801 cgccttggaa atggccgtgt cgctgaacgc gtttgaacag gcggaaatga tttatcagaa
     7861 atggcggcag attgagccta taccgggtga ggcgcaaaaa cgggcggggt ggctgcggaa
     7921 cgtgctgagg gaaagaggaa atcagcatct ggacggactg gaagaagtgc tggctcaggc
     7981 ggacgaagga cagaaccgca gggtgttttt attgttggca caagccgccg tgcaacagga
     8041 cgggttggcg caaaaagcat cgaaagcggt tcgccgcgcg gcgttgaaat atgaacatct
     8101 gcccgaagcg gcggttgccg atgtggtgtt cagcgtacag ggacgcgaaa aggaaaaggc
     8161 aatcggagct ttgcagcgtt tggcgaagct cgatacggaa atattgcccc ccactttaat
     8221 gacgttgcgt ctgactgcac gcaaatatcc cgaaatactc gacggctttt tcgagcagac
     8281 agacacccaa aacctttcgg ccgtctggca ggaaatggaa attatgaatc tggtttccct
     8341 gcacaggttg gatgatgcct atgcgcgttt gaacgtgctg ttggaacgca atccgaatgc
     8401 agacctgtat attcaggcag cgatattggc ggcaaaccga aaagaaggtg cttccgttat
     8461 cgacggctac gccgaaaagg catacggcag ggggacgggg gaacagcggg gcagggcggc
     8521 aatgacggcg gcgatgatat atgccgacag aagggattac accaaagtca ggcagtggct
     8581 gaaaaaagtg tccgcgccgg aatacctgtt cgacaaaggt gtgctggcgg ctgcggcggc
     8641 tgtcgagttg gacggcggca gggcggcttt gcggcagatc ggcagggtgc ggaaacttcc
     8701 cgaacagcag gggcggtatt ttacggcaga caatttgtcc aaaatacaga tgctcgccct
     8761 gtcgaagctg cccgacaaac gggaggcttt gagggggttg gacaagatta tcgaaaaacc
     8821 gcctgtcggc agcaatacag agttacaggc agaggcattg gtacagcggt cagttgttta
     8881 cgaccggctt ggcaagcgga aaaaaatgat ttcagatctt gaaagggcgt tcaggcttgc
     8941 acccgataac gctcagatta tgaataatct gggctacagc ctgctttccg attccaaacg
     9001 tttggacgaa ggcttcgccc tgcttcagac ggcataccaa atcaacccgg acgataccgc
     9061 tgtcaacgac agcataggct gggcgtatta cctgaaaggc gacgcggaaa gcgcgctgcc
     9121 gtatctgcgg tattcgtttg aaaacgaccc cgagcccgaa gttgccgccc atttgggcga
     9181 agtgttgtgg gcattgggcg aacgcgatca ggcggttgac gtatggacgc aggcggcaca
     9241 ccttacggga gacaagaaaa tatggcggga aacgctcaaa cgtcacggca tcgcattgcc
     9301 ccaaccttcc cgcaaacccc ggaaataatg caggtccatc ctttcagacg gcataaggtt
     9361 tgccgggaag ctgcggcatt cgggcaaacg gcacgcagtt cgcacgcgtt ttgcacggca
     9421 caccgaaccc atcggccggc aggatggcat ccgttaagga aattctgatg aaacacactg
     9481 tatccgcatc ggtcatcctg cttttgaccg cttgcgcgca attacctcaa aataacgaaa
     9541 acctgtggca gccgtccgaa cacacccgca gtttcacggc agaagggcgg ttggcagtga
     9601 aagcggaagg gaaaggttcg tatgcaaatt tcgattggac ataccaaccg cccgtggaaa
     9661 ccatcaatat caatacccct ttgggcagta cgctcgggca gttgtgtcaa gacagggacg
     9721 gcgcattggc agtggacggc aaaggaaatg tctatcaggc ggaaagtgcg gaagaattga
     9781 gcaggcagct ggtcggtttc aaactgccaa tccaatatct gcatatctgg gcagatggca
     9841 ggcgtgtggc gggcgcgcct taccgcatcc tgccggacgg catattggaa caatacggtt
     9901 ggactgtcgg cagaaccgcc gacagtgggg ggcaagtccg aacgttgcaa ctgaataacg
     9961 gaaatttgaa catcaggctg gttttcaccg aaatcggtat gccgtctgaa accgaaaccc
    10021 cggaacgctg tgcggcgcgc acgagataag gcggacagat gaatattgcg gacggacggc
    10081 aggcgtttcc cgcacctgca aaactgaatc tcgatttgag gattaccggc aggcgggaag
    10141 acggttatca caatatcgaa agcatattct gcctgataga tttgcaggat accgtatatt
    10201 tgaaaccgag ggacgacggc aaaatcatcc tgcacaatcc tgtcggcgga ataccgcagg
    10261 aagccgattt gagctaccgt gccgcatcgt tgctgcaaaa atatgcgcgc aaccccgccg
    10321 gcgtggaaat atggctggac aaaaaaatcc cgacaggggc gggtttgggc ggcggaagct
    10381 cggatgcggc aaccgttttg ctggtgttga accgttggtg gcagtgttcc cgaacgacgc
    10441 agcagctgat tgacttgggc gcaactttgg gtgcggacgt accatttttt attttcggaa
    10501 aaaatgcttt tgcaagcggg ataggtgaaa aactgatagg aatggatatt ccgaaacagt
    10561 ggtatgtcat cgtcaaaccg cccgtccacg tttccactgc aaaaattttc acacacgaag
    10621 gcttgacacg aaattccgcc tcaagcataa tgccgacttt ccaaaacctg caaccgttta
    10681 gaaatgatat gcaggcagtt gtatttaaag aataccctga agtttggaaa gcctattccg
    10741 aattgtcccg atatggattt gccttaatga caggttctgg tgcgtgtgta ttcacggcgt
    10801 gtcaagatag ggatagcgca tacaatatat accgacaagt ttcaggtttg tacgaggcat
    10861 atttggcaga gggtctttca aaacatcctt tgttgtccgt ataaacattg ttggggagtc
    10921 gtcaagcggt taagacactg gattttgatt ccagcatgcg aaggttcgaa tccttcctcc
    10981 ccagccaagc caaacgagtt ggggagtcgt caagcggtta agacactgga ttttgattcc
    11041 agcatgcgaa ggttcgaatc cttcctcccc agccagccct ttttggggag tcgtcaagcg
    11101 gttaagacac tggattttga ttccagcatg cgaaggttcg aatccttcct ccccagccag
    11161 ataaaagcgt gtaagcctgc ttacacgctt tttatttcat agaaataaaa atattgaaat
    11221 gcctttgttt gtgtcggatg ttgcaggtat aatgtcgggc ttggtacaag cagagggaag
    11281 cattgtgttt tctgagcgga agttaaacat aaaatcaggt gagaatatgg ctgcgtacga
    11341 cagtttgatg gtatttacag gcaatgccaa tcccgaattg gcacaacgtg ttgtcaggca
    11401 tttggacatt tctttgggca atgcttccgt atccaagttt tcagacggcg aagttgccgt
    11461 cgaactgttg gaaaacgtac gcgggcgcga tgttttcatc cttcagccga cctgtgcgcc
    11521 gaccaatgac aacctgatgg aaatcctgac gatggcggat gcactgaagc gtgcttcggc
    11581 aggtcgtatt accacagcca ttccgtattt cggctatgcg cgccaagacc gccgtccgcg
    11641 ttccgtccgc gttccgattt ctgccaaact ggtggcaaat atgctgtatt cggcagggat
    11701 cgaccgtgtt ttgactgtcg atttgcatgc cgaccagatt caaggtttct tcgatattcc
    11761 agtggacaat atttatgcca ccccgattct gttgaacgac atcaaacaac agcggattga
    11821 aaatctgacc gtcgtcagcc cggacatcgg cggtgtcgtc cgcgcccgcg ccgtggcaaa
    11881 atccctgaat gccgacttgg caatcatcga caaacgccgc ccgaaagcca atgtggcgga
    11941 agtcatgaac atcatcggcg atattcaagg cagaacctgt ctgattgtgg acgatatgat
    12001 tgacactgca aatacgctgt gcaaagccgc cgtcgctctg aaagagcggg gggcggaacg
    12061 tgtcttggca tatgccagcc acgccgtatt ctccggagag gcggtcagcc gtatcgcctc
    12121 atccgaaatc gaccaggtgg tcgtaaccga taccattcct ttgtctgaag cggctaaaaa
    12181 ctgcgaccgt atccgtcagg taacgattgc cggtctgttg gccgaaaccg tccgccgcat
    12241 cagcaatgaa gaatccgtct catatctttt caatgaagaa gtgatgacag gcagtatgtt
    12301 gctgccataa gcccgaagcc gtcttaagct ggtcgcggcc gatgacggcg attttaccta
    12361 acttggagta tttaacatga cttatgaaat tcaagcctct gttcgtgaag cacaaggcac
    12421 tggtgcgagc cgccgcctgc gtcgcgaagg ccaaatcccc ggcattctgt acggtgaagg
    12481 tcaagagcct gttgcaatcg ctgtggatca caaaaccgta ttctacgcat tggaaaaaga
    12541 atctttccat accgcgttga ttaaattgtc tctgaacggc gaaaccaaag acgttatcgt
    12601 ccgtgatttc caaatgcacc cgttccgccg cgaagttcaa cacatcgact tccaagctgt
    12661 gaaagccgat caacttgtac gcatccgtgt tcccctgcac atcgttaacg ctgaaaattc
    12721 ccaagcggtc aaactgcaag gcggccgtgt atctctgttg aacacgactg ttgaagtgct
    12781 tgcattgcct gccaatatcc ctgctttctt ggatttggat tgtgccgaag tggttgccgg
    12841 cgacattctg catctgtccg acatcaaact gcctgaaggt gtagaaagcg tttccttgaa
    12901 acgtaacgaa aatctggctg ttgccaccgt taccggtaaa aaacgctaat tgatttcagc
    12961 agcagggcgg cgcgtatgca atacgtaccg ccctgttgtt ttatgccgtc tgaaccgtgt
    13021 ttcagacggc atttctttat ttgttggaaa aacgggatat ttgaaacggc agattactgt
    13081 cccgtcagac gcatccaaag cctttgccac cggcttcttt tttctacatt ttccagtgcg
    13141 acaatttctt tttcggtaat ggtgtatccg ttttgcctga ttttgatttt tcctaaaatc
    13201 tgtccctttt ttaccggggc gggaatcggc tgtatggttt ctagaatttg ttctgccatt
    13261 ttcgcttcct tatgtggcag agtgatgtag gcttctttga ggaagcctgc gcggacggtt
    13321 tttttgctgc ctccggaaat ttggatttgg gcaacggttt tgcctttcgg atatattttg
    13381 ggcgtatcga aggcctgcaa tgcccagttc agcagcttgc tgttgtctga tgcgcgtgtt
    13441 tccgccgatt ccgaacccaa tgtgatgaca aggatgtgcc tgccgttgcc ggagtatgac
    13501 acggcaaggt tgtagccgcc gctttctgtg tgtccggctt tcagaccgtt tacattgttg
    13561 tccctatata aaaggatatt gcggttgttt tgctctatat ttttgaattt gaaagatttg
    13621 atggaaaaca gcgggtaata ttccggaaag tcgcgcatca atgcttcaga cagctgggcg
    13681 aggtctttgg cggtggaaac ctgtccttct ctactcaagc ctgtcgggtt tttgaataca
    13741 gtgttcttca tgcccaagcg tcgggcttct ttgttcattt gttgcacaaa attttcaatc
    13801 gagccgttgc ccagccggcc ggcaagggtt agggcggcat cgtttgcgga tagggcaatc
    13861 atgcctttta agagtttgtc ggtgctgacc gtatcgccgg gacgtacaaa cattctgctt
    13921 ccttctgaag cccatgcgga ttcgggtatt tttaagtttt cttcagattg gatatttccc
    13981 gatttcatgt ttttgaaaac caaatatgcg gtcatcagtt gggttagtgc cgccggttcg
    14041 acaggggtat tgatgttttt ggcggataaa gtctgtctgc tttgaaggtc gataacgatg
    14101 tgtgccgctg tgagggtttc gggtgtttgg agcgtggggg cggcgtgtgc cgtcggtctg
    14161 ttgggcacgg gcgatgcagc cgttgcgtga gaaatgccta agatgatgga aagcaggacg
    14221 ggcaggattt tatgtgctgt catgaaatat tctaattgtg tgcgtgtttc agtctgccga
    14281 ttatacgctt agggtgtctg atcgggcgga tttttcttgc tttcgcgccg tcttgggcgt
    14341 atggttttgg gttttgcgat tttaataaac cgattatccc atattgaatt atgaacacgc
    14401 cccttcctta ttccaattac ctcatccgca tcctgacggc atctgtctat gatgtcgcgg
    14461 tcgaaacgcc tttggaaccg gcacgcagcc tttctgtacg tttgaaaaac aacatccttt
    14521 tgaaacgcga agatttgcag ccggtttttt cgttcaaaat acgcggcgcg tacaacaaaa
    14581 tgtccaagtt gccgaaagat gcgctcgctt gcggcgtgat tgcggcaagc gcgggcaatc
    14641 atgctcaagg cgtggcattg tccgcacagc gtttgggctg ccgtgccgtt atcgttatgc
    14701 cggagactac gccgaaaatc aaagtggatg cggttaagag ccgtggcggc gaggtggttt
    14761 tgcggggcgt ttcatacaac gatgcctacg attatgcgat ggagttggcg gaaaaagaag
    14821 ggttaaccta tatcgcgccg tttgatgatc ctgatgtgat tgcgggacag gggacggtgg
    14881 ggatggaaat tgtcagccag catcccgatc caatccgcgc cgtattcgta ccgataggcg
    14941 gtggcggttt ggcggcgggc gtggcggcat ttatcaagca ggtccgtccc gaaatcaaag
    15001 ttatcggcgt tcagaccaac gattcctgct gtatgaagca gtcggtcgaa gcgggtgaaa
    15061 tcgtccattt gaaagatgtc gggctgtttt cagacggcac tgcggtcaaa gtcgtcggaa
    15121 acgaaacctt ccgcctctgc aaagaacttt tggatgaaat cattacagtc gataccgatg
    15181 cggtttgcgg cgcggtcaag gatattttcg atgacacgcg cagcattacc gagccggcgg
    15241 gcgcgttggc gttggcgggt ctgaaagcct atatcgcccg agaaggcgcg gaaaaccaaa
    15301 ccctgattgc cgttaccagc ggtgcgaata tgaattttca ccgtttgcgc catgtgtcgg
    15361 agcgtagtga attgggcgag ggcaacgaag gtatttttgc cgttaccatc cccgaagaac
    15421 gcggcagctt ccttaagttt gtcaatatat tgggaaatag gaatattacc gagttcaact
    15481 accgctacgg agacgatgaa aaagcgcata tctttgtcgg acttcaagcg gcaggtccgc
    15541 aggatttggc ggttatcggt agccggttgg atgaggcggg attgcccaat gtcgacctga
    15601 ccgatgatga gattgccaaa atccatatcc gctatatggt cggagggcgg acggacaaag
    15661 tagaaaacga gcgtctggtc agttttgagt ttccggagcg tccgggggcg ttggcgcgtt
    15721 ttttgaacca tatgcagggc ggttggaaca ttacgctctt ccattaccgc aaccacggtg
    15781 cggattacgg gcggattttg gtcggtatcg atgtgccgcc gcacgatgcc gccgcatttg
    15841 acggtttctt ggaaagtctg ggatacagct atcacgagga aacgcaaaat gccgcgtaca
    15901 agctgtttct tgcctgacgc ttgaaagcac aatgccgtct gaaagccttt cagacggcat
    15961 tgcgctttca tggttaaatc gaatattcaa tcagttcgtt ttgagagaag acataaacct
    16021 gtttcggaat cagcttcaat tctttgcctt cggcgattgg gtaacgcgcg gcatcgctgc
    16081 cagccagcgt gatatgcacg tcttgtttgc cgtgtttcac caaaatatgc gtcaatgccc
    16141 cgacggcgtg gactttttcg atttcggcac aaatcatcgg tgtttcgtgt tcggcggcga
    16201 tctgccattc gtgcgggcgg atatagccgg tggcggtttg ttcctgccat ttgtattgtg
    16261 cgtccaattt ccacgcgaag ccgttataat gccagaagcc tttttcgata cgtccttcaa
    16321 aagcgtcggt ttcgccgagg aactcggtaa cgaaggcatt ttcgggtttg cggtaaatag
    16381 cttcggcgct gccggtttgt tcgattttgc cgtggttcat cacgacgatt tcgtcggaaa
    16441 cttcgagggc ttcttcttgg tcgtgcgtaa ccagaatgct ggttacaccc aggttgtgat
    16501 ggatgtcgcg cagccaggtg cgtaattctt tgcgtacttt ggcgtccaac gcgccgaagg
    16561 gttcgtccaa aagcaggagt ttcggttcga ccgcaagcgc acgggcgagg gcgatgcgct
    16621 ggcgttgccc gccggagagt tggtgcggat aggattttgc caaatgcgag agttgtacga
    16681 gtttcagcaa ttcttcgact ttagtgcaga tttgttcttt ggacgggcgt tcggacttgg
    16741 gcaatacggt caaaccgaaa gcgacgttgt caaacacgtt catgtggcgg aagagggcgt
    16801 agtgttggaa cacgaagccg actttgcgct cgcggacgtg tttggcggtt acgtcttgcc
    16861 catcaaacag aatcttgccg ccgtcggcgt tttccagtcc ggcgataatg cgtaaaagcg
    16921 tggttttgcc gcagccggac gggccgagca gggaaacgag tttgccggtg gggacgttga
    16981 ggttgatgtt tttcagcgcg tgaaaattgc cgaagtgttt gtttaagttt tggatggtga
    17041 tactcatact gcgttccttt cggcggcggc gagttttttg tcttgtaatt tggtgatgat
    17101 gttctgcacc gccagcgtcg ccagtgccaa aagtgccaat acgccggaga gggcgaatgc
    17161 gccggtgaag ttgtattcgt tgtagaagat ttcgaccaaa agcgggacgg tgttggtttc
    17221 gccgcgtatg tgtcccgata ccacgctgac cgcgccgaac tcgcccatcg cgcgggcgtt
    17281 ggtgaggatg atgccgtaga gtaacgccca tttgatgttg ggcagggtaa cgcgccaaaa
    17341 catctgccag ccgcttgcgc cgagtatcaa tgccgcctgt tcttcgctgt cgccctgagc
    17401 ctgcatcagc gggatgattt cgcgcgcgac aaaggggaag gtaacgaaca gcgtcgccag
    17461 aataatgccg gggatggcga agataatctg tatgccttgc gcttcgagcc agccacccaa
    17521 tgccgtgtgc gcgccgaaca ataagacgaa cattaaaccg gccaccacgg gcgatacgga
    17581 aaacggcaaa tcgagcaggg tagtcagcaa ctgcttgccg cgaaaatcaa aacgggtcag
    17641 cagccacgcc atcgccacgc ccaagacggc attgacgggg acgacaatca gcgcggtaat
    17701 cagcgtcaat ttgatggcag accacgcttc gggatcgttt aaggatttca ggtacaaatc
    17761 ccaaccgcct tttaaggctt cgtaaaatac ggcgacgagc ggcacgacca gcatcagcag
    17821 cagaaagccc agcgcggcgg caatcagcaa cacgcgcagc cgacgcggtt cggtcaggtt
    17881 gggattggcg gaatagggtt tcatggcggt ggtgttctct ggttggtttt aaaatgtttg
    17941 gaggtcgtct gaaaaactgt gtttttggga gcggattcgt tttcaga
//
LOCUS       DAWHXZ010000015        17823 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000015 DAWHXZ010000000
VERSION     DAWHXZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17823
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.015"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..555)
                     /gene="rfbC"
                     /locus_tag="WIE35_000348"
     CDS             complement(<1..555)
                     /gene="rfbC"
                     /locus_tag="WIE35_000348"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ4153321.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLPLSR"
     gene            complement(609..1475)
                     /gene="rfbA"
                     /locus_tag="WIE35_000349"
     CDS             complement(609..1475)
                     /gene="rfbA"
                     /locus_tag="WIE35_000349"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ4153322.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            complement(1581..2621)
                     /gene="rfbB"
                     /locus_tag="WIE35_000350"
     CDS             complement(1581..2621)
                     /gene="rfbB"
                     /locus_tag="WIE35_000350"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ4153323.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIQNTQDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIDSAGEF
                     IQTNIVGTFNLLEAARAYRQQMPSEKHEAFRFHHISTDEVYGDLSGTDDLFTETAPYA
                     PSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKP
                     LPVYGDGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDARYAVDTAKIRRDLGWQPLETFESGLRKTVQ
                     WYLDNKTRRQNA"
     gene            complement(2622..3665)
                     /gene="galE"
                     /locus_tag="WIE35_000351"
                     /pseudo
     CDS             complement(2622..3665)
                     /gene="galE"
                     /locus_tag="WIE35_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(3658..4660)
                     /gene="dcm"
                     /locus_tag="WIE35_000352"
                     /pseudo
     CDS             complement(3658..4660)
                     /gene="dcm"
                     /locus_tag="WIE35_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(4691..6964)
                     /locus_tag="WIE35_000353"
     CDS             complement(4691..6964)
                     /locus_tag="WIE35_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ4153324.1"
                     /translation="MNITQILSQELSATTAQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIACRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALGRLKEAADTDAITVFARNLKDLLLVAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(7029..7679)
                     /locus_tag="WIE35_000354"
     CDS             complement(7029..7679)
                     /locus_tag="WIE35_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ4153325.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMHQFEDMDKAYEYYNSLP"
     gene            complement(7676..8473)
                     /locus_tag="WIE35_000355"
     CDS             complement(7676..8473)
                     /locus_tag="WIE35_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ4153326.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(8473..9636)
                     /gene="ctrB"
                     /locus_tag="WIE35_000356"
     CDS             complement(8473..9636)
                     /gene="ctrB"
                     /locus_tag="WIE35_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ4153327.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(9651..10826)
                     /gene="ctrA"
                     /locus_tag="WIE35_000357"
     CDS             complement(9651..10826)
                     /gene="ctrA"
                     /locus_tag="WIE35_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ4153328.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            10969..12102
                     /gene="neuC"
                     /locus_tag="WIE35_000358"
     CDS             10969..12102
                     /gene="neuC"
                     /locus_tag="WIE35_000358"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ4153329.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTYKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGAAVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            12106..12792
                     /gene="neuA"
                     /locus_tag="WIE35_000359"
     CDS             12106..12792
                     /gene="neuA"
                     /locus_tag="WIE35_000359"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ4153330.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            12793..13842
                     /gene="siaC"
                     /locus_tag="WIE35_000360"
     CDS             12793..13842
                     /gene="siaC"
                     /locus_tag="WIE35_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ4153331.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIS"
     gene            13994..17107
                     /locus_tag="WIE35_000361"
     CDS             13994..17107
                     /locus_tag="WIE35_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ4153332.1"
                     /translation="MAVIIFVNGIRAVNGLVKSSINTANAFAEEGLDVHLINFVGNIT
                     GAEHLSPPFHLHPNVKTSSIIDLFNDIPENVSCRNIPFYSIHQQFFKAEYSAHYKHVL
                     MKIESLLSEEDSIIFTHPLQLEMYRLANNNIKSKAKLIVQIHGNYMEEIHNYEILARN
                     IDYVDYLQTVSDEMLEEMHSHFKIKKDKLVFIPNITYPISLEKKEADFFIKDNEDIDN
                     AQKFKRISIVGSIQPRKNQLDAIKIINKIKNENYILQIYGKSINKDYFELIKKYIKDN
                     KLQNRILFKGESSEQEIYENTDILIMTSQSEGFGYIFLEGMVYDIPILAYNFKYGAND
                     FSNYNENASVFKTGDISGMAKKIIELLNNPEKYKELVQYNHNRFLKEYAKDVVMAKYF
                     TILPRSFNNVSLSSAFSRKELDEFQNITFSIEDSNDLAHIWNFELTNPAQNMNFFALV
                     GKRKFPMDAHIQGTQCTIKIAHKKTGNLLSLLLKKRNQLNLSRGYTLIAEDNSYEKYI
                     GAISNKGNFEIIANKKNSLVTINKSTLELHEIPHELHQNKLLIALPNMQTPLKITDDN
                     LIPIQASIKLEKIGNTYYPCFLPSGIFNNICLDYGEESKIINFSKYSYKYIYDSIRHI
                     EQHTDISDIIVCNVYSWELIRASVIESLMEFTGKWEKHFQTSPKIDYRFDHEGKRSMD
                     DVFSEETFIMEFPRKNGIDKKTAAFQNIPNSIVMEYPQTNGYSMRSHSLKSNVVAAKH
                     FLEKLNKIKVDIKFKKHDLANIKKMNRIIYEHLGININIEAFLKPRLEKFKREEKYFH
                     DFFKRNNFKEVIFPSTYWNPGIICAAHKQGIKVSDIQYAAITPYHPAYFKSPKSHYVA
                     DKLFLWSEYWNHELLPNPTREIGSGAAYWYALDDVRFSEKLNYDYIFLSQSRISSRLL
                     SFAIEFALKNPQLQLLFSKHPDENIDLKNRIIPDNLIISTESSIQGINESRVAVGVYS
                     TSLFEALACGKQTFVVKYPGYEIMSNEIDSGLFFAVETPEEMLEKTSPNWVAVADIEN
                     QFFGQEK"
     gene            17188..17823
                     /locus_tag="WIE35_000362"
     CDS             17188..17823
                     /locus_tag="WIE35_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ4153333.1"
                     /translation="MYSEQGINNTINISTTSLTNATQLTVIGNNNSVYIGNNCKIVSS
                     NIRLKGNNITLFIADDVEIMGLVCSLHSDCSLQIQAKTTMGNGEITIAEKGKISIGKD
                     CMLAHGYEIRNTDMHPIYSLENGERINHGKDVIIGNHVWLGRNVTILKGVCIPNNVVV
                     GSHTVLYKSFKEPNCVIAGSPAKIVKENIVWGRKMYHSTMYDDPTLNEFYK"
BASE COUNT         5092 a         4037 c         3774 g         4920 t
ORIGIN      
        1 tctggaaagc ggaaggcgga gcttttcggc ttgcgcccac gttttgccgg caaggtcttt
       61 gggcgacagc agcggcgcgg tttgaagcgg ccagcctatg ccgactgtcg ggtcgttcca
      121 tattaaaacc tgttcggctt caggcttgta atagtcggtg catttgtaca cgacttccgc
      181 cgcgtcgccc aaaacgcaaa acccgtgcgc gaagccttcg ggtatccaaa gctggcattg
      241 gttttgcgcg gacagggttg cgcccgccca tttgccgaaa gtcggcgaac cttcgcgcat
      301 atcgacggcc acgtcgaaca cttcgccgac aactacgcgt acgagtttgc cttgtgtgtt
      361 ttcggtttgg tagtgcaggc cgcgcaatac gcctttgccg gatttggagt ggttttcctg
      421 cacgaaggtg cgttcgcaga cttgggtttt aaaccactcg tcgcggaagg tttccataaa
      481 aaagccgcgc gcgtcgccga agacttgggg ctcaagcagt tttacgtcag gaatggcggt
      541 atcaatgatg ttcatctttt tatctttcat ctaaaggccg tctgaaaagt ttcagacggc
      601 ctcaaacatt attttttcaa caggcgcagc aaatattggc cgtattggtt tttcgccatc
      661 gggcgcgcca attcttccag tttttcatcg gaaagccaac cgttgcgcca agcgatttct
      721 tccaggcagg cgatgtgcag gttttggatg ttttgcacgg tttggacgaa cgaagccgcc
      781 tcgtgcaggc tttcgtgcgt accggtgtcc agccacgcga aaccgcgtcc caatatttga
      841 acggagagcg agccgtcttc caaatacatc cggttgaggt cggaaatttc caattcgccg
      901 cgtgcggacg gtttgagctg tttggcgaac tcgacggcgc ggttgtcgta gaaatacaag
      961 ccggttaccg cccaatcgga tttgggccgt tgcggttttt cttcgatgga aacggcgcgg
     1021 aagttttcgt taaattcaac cacgccgaaa cgttcggggt ttttgacctg ataagcaaac
     1081 acggttgcgc cgtgcgtttg cgctgccgcc tgtttcaatg tttgcgtaaa cgactgaccg
     1141 taaaaaatat tgtcgcccaa aaccaagcaa acattgtcgt tgccgataaa ttcttcgccg
     1201 atgataaatg cctgtgccaa gccgtccgga ctgggttgca cggcataact gatggaaatg
     1261 ccgaaatcgc tgccgtcgcc aagcaggcgt ttgaaagagg cgttgtcttc aggcgcggta
     1321 atcaccaaaa tatcgcggat tcccgccagc atcaaaaccg acaaggggta ataaatcatc
     1381 ggtttgtcgt acacgggcag gagctgtttg gatacgccgc gcgtgatggg gtagaggcgc
     1441 gtgccgctgc cgcctgccag tatgatgcct ttcatctttt ctttcttcct ttgcgatggg
     1501 ttttcagatg gcattgcgtc gggatgccgt ctgaaaactt attttccggt gcctaaacgt
     1561 tccaaacgat agctgccgtt ttatgcgttt tgccgccgag ttttgttgtc caagtaccat
     1621 tgcactgttt tgcggaggcc ggattcaaag gtttccaaag gctgccagcc caaatcccgt
     1681 ctgattttcg ccgtatcgac ggcgtagcgg gcatcgtggc cagggcggtc ttgtacgaaa
     1741 gtaatcaaat cttcataacg cgccacgccg gccggttttt cgggagcgag ttcttccagc
     1801 agggcgcaga tggttttgac gacttcaata ttggcttttt cattgtggcc gccgatattg
     1861 taggtttcgc cgacaacacc ttcggtaaca acctgataca gtgcgcgcgc gtggtcttcg
     1921 acaaacagcc agtcgcggat ttgcataccg tcgccgtaca caggcagcgg tttgccgtca
     1981 agcgcgttca gaatcatcaa aggaatgagt ttttccggaa aatggtaagg accgtagttg
     2041 ttggagcagt tggttacaat ggtcggcaag ccgtaagtac gcaaccacgc gcggacgagg
     2101 tggtcgctgg acgctttaga ggcagagtaa gggctggacg gcgcgtaggg cgcggtttcg
     2161 gtaaacaaat cgtctgtgcc gcttaaatcg ccataaactt catcggtaga aatatggtgg
     2221 aaacggaagg cttcgtgctt ttcagacggc atttgctgcc ggtaggcacg cgcagcttca
     2281 agtaggttga atgtgccgac gatattggtt tggataaact cgcctgccga atcgatagag
     2341 cggtcgacat ggctttccgc tgccaagtgc atcacggcat cgggccggtg ttgtgcgaat
     2401 acgcggtcga gttcggcgcg gtcgcaaata tccacttgct caaaagcgta gcggggatta
     2461 tcggcaatgt cggtcagcga ttcgagattg cccgcgtagg tcagtttgtc gaggttgacg
     2521 acggaatctt gggtgttttg aatgatatgg cggacaacgg cagagccgat aaagcccgcg
     2581 ccgccggtaa cgaggatggt ttttttgttt gcggtttgca tttaatcccc atatccgttg
     2641 gggttgcggc tgacccaacg ccacgaatcc tccatcattt gcgcaaggct gcgtttggtt
     2701 tcccaacctg tctcttgttt ggtatgggac gggtcggcaa aggaacacgc caagtctccg
     2761 gcgcgtcggg gttggatttg atagggaata tttaagccgg atgcggcttc aaaggcacgg
     2821 atgatttcca aaaccgaata ggcgcgcccc gaacccaagt tgaacaaatg tacgccggca
     2881 acaccgcctt tcgccttcat tgccgcgata tgcccttctg ccaaatccat cacatgaata
     2941 tagtcgcgca ttcccgtacc gtccggggtc ggataatcgc cgccgaatac cgacagttgc
     3001 ggcagcctgc ccgaagccac ttggcagata tagggcaaaa ggttgttggg aataccgttg
     3061 ggctgttcac cgatcaagcc gctttcgtgc gccccaatcg ggttgaaata gcgcaacaaa
     3121 atcacgctcc aacgcggatc ggccttttgg atgtcggtta acatccgttc caccatcgct
     3181 ttggatgcgc cataaggatt agcggtatcg cccgggcgca tatcttccgt atagggcatt
     3241 ttacgtatca tggcgcgacg cattaaagta aacgccaaag cgcgtttata ctggttatcc
     3301 agtttcccta tatttttacg ttacaaatca agttgttggt attcatcgtg aaaatagaat
     3361 ttttcggcat aacgctgact cataaaaccg gcatgcgggc tacctgaaaa aatcattgcg
     3421 ccatcatcgt catttaatgt ttcatggttg ttttctatta atgctaaagc aatttggtaa
     3481 ttaattgcca aaatatcgtt agtaatcaca cgatagccgc gttttttggc ttcgtaggca
     3541 aaggaacagc ctccactaaa tacatctgca actgtatcta catcagacgg aagctggtca
     3601 caaatccagg aagctatttt ctctttatta ccgatatagt taattttcgg atattgctta
     3661 ttcattttca ctcattttta aaatactttc agcaatagct tttgccaata aaggcggtac
     3721 ggcgttacca acctgctgct gctgggcaat tttgctgccg caaaaaataa aattatcagg
     3781 gaaagattgt aaggcagcta attcacgaac ggttaacgcc cgattctgtt catagtgaaa
     3841 aactttgcgc atatctcctg taatacaaac ggctggtttt gttgctgttg taacggatgt
     3901 atttacggat atcacctgtt ttcggacgta atggttcagg aatatcgtta cggttacctc
     3961 catttttaac aaatgccatt ttttctaaca tttgtgccga atgattcata gcttcatgat
     4021 ttgcaacgtg tggattgctt tcgccagcag ccagttttgg aaaatgtcct attgctgatc
     4081 caacagtctg atgggaaatc tgcaaaggtt cgggaaagga aattttgcct ttatccctcc
     4141 tcccgataaa tatcactcgg ctacgtatct gaggaacacc gaaatcggct gcactcagta
     4201 tcttacattc caccgaataa ccgatattct gaaatgcttg aataatctca atacgtgttt
     4261 tacctgaatt gtgtgtatag agtcgcgcta cattttccat aacaaaaaaa tatggttgga
     4321 caattttaac tattcggaca aactctttaa ataaatggtt gcgtgggtca tctgtaaatg
     4381 tccgtccaat ctttcctgcc atactaaaac cttgacaagg tggtcctcca ataatcaaat
     4441 caactgcttg tccgttaaga caattgatta aatcttgttc ggttagtgtg gttaaatctt
     4501 tttgcagtaa ttgatgatgg gggaagttgg tacggtaagt ctgacaataa tcagactcca
     4561 tttcaacaga aagcaattgt tggaatccgg cttgttcaaa acccaaggat aggcctcctg
     4621 ctcctgagaa aaggtcaata taggtaagtg gtgtgtgcat aagtcaaaat ccataaactc
     4681 ttcgattatt ttaccgcttc agcttcgcaa acgcatccgc catcgccgaa ttggctgggg
     4741 cgcggtcgtt gcgttggggt ttgcggccgg ctgtccgttc gcggctgcgg ttttcagacg
     4801 gcattttatg ttttgcgccg cccggttcgt catccaagcg catggtcagc gcgatgcgtt
     4861 tgcgtgcagc atcgacttcc agcactttca ctttcaccac gtcgccagct ttcaccactt
     4921 cgcgcgggtc ttggacgaac ttgttggaca gggcggagat gtgcaccaag ccgtcctgat
     4981 ggacgccgat gtccacgaac gcgccgaagt tggcgacgtt ggaaaccacg ccttcgagta
     5041 tcataccgac ttgcaagtcg ctgatttcgt ggataccttc ggcaaacgat gccgtctgaa
     5101 actcgccgcg cggatcacgg ccgggttttt ccagttcgga caggatgtcc aaaatggtcg
     5161 gcaggccgaa gcgttcgtcg gtgaagtcgg acgctttgat ttgcttcacg cgctcgcggt
     5221 tgccgatgag ttcggcggcg ctaatgcctt gttgcgccag cattttggcg acgacgggat
     5281 aggcttcggg gtggacggcg ctcgcgtcca acggctcttt gccgccgtta atccgcaaaa
     5341 agcccgccgc ctgctcgaag gttttttcgc ccaaacgcgg tactttcagc aattttttgc
     5401 ggctgtcgaa cgcgccgttt tcatcgcggt aggcgacaat gttttgggca agggtttgat
     5461 tcaaaccgga aattcgcgcc aagagcgggg cggaggcggt gttcacgtcc acgccgacgg
     5521 cgttcacgca gtcttcgacc accgcgtcca gcgatttggc gagctggctt tggttcacgt
     5581 cgtgctgata ctgccccacg ccgatggatt tggggtcgat tttgaccaac tcggcaagcg
     5641 ggtcttgcag cctgcgggcg atggacaccg cgccgcgcag ggaaacgtcc aagtcgggga
     5701 actcgcgcgc cgccagttcg gacgcggaat aaatcgacgc gccggcttcg gacacgacga
     5761 ttttatgcag ccccattttc gacatttcac gcaccagttc gcccgcgatt ttgtcggttt
     5821 cacggctggc ggtgccgtta ccgatggcga tgagcttcac gccgtgctgt ttaatcaggc
     5881 gcgacagcgt tgccaacata ttgttttctt gatgcaaata gacgatgacg gtatccagca
     5941 gcttgcccgt atcatccacc acggcgcatt tcacgccgtt gcggtagccg gggtcgagac
     6001 ccagcgtggt cagccgtccg gcgggcgcga cgagcagcaa gtctttgaga ttgcgggcga
     6061 acacggtaat cgcgtcggtg tcggcggctt ctttcagacg gcctagggct tcaagttcca
     6121 acgacaaaaa gattttcgcg cgccaagtca gacgcacggt atcgcgcagc catttgtggc
     6181 cgtctgaaac cttgaagcgg caggcgatga tttgctcgta ttcgctttgc tgcgtaatcg
     6241 gcgtgtcgtc gggctgatat ttgagcgcga tgttcaacac gccttcgttg cgtccgcgca
     6301 aaaccgctag cgcacgatgg ctgggcatcg cacgaatagg ttcgcggtgg tcaaaataat
     6361 cgctgaattt ttcgccttcg gtttctttgc ctccaacaac ttgcgcgtgg atttcggctt
     6421 cgttccacag cttgtcgcgc agcgtgccga tgagttccgc gtcttcggca aactgctcca
     6481 tcagaatcgc acgcgcgccg tccaacgcgg ctttggcatc ggggacgttt tcgttcaggt
     6541 agccttgtgc ggcggcttcc acgtcctgcg actgctcggc aagcaacacg tccgccagcg
     6601 gctgcaaacc gtgttcgcgc gcgatttgcg ctttggtgcg gcgtttgggt ttgtagggca
     6661 gatacaggtc ttccagcgcg gttttgttat cggcggcttc gatttgcgcc ctgaggtcgt
     6721 ctgaaagctt gccttgctct tcaatgcttt ttaacacaac ggctttgcgc tcttccaact
     6781 cgcgcaggta ttgcagccgc tcggcaagcc ggcgcagctg cgtgtcgtcc agcccgccgg
     6841 tggcttcttt acggtaacgc gcgataaacg gcacggttgc gccgtcgtct aaaagctcaa
     6901 tagcggcatt gatttgcgcg gtagtcgcgg aaagttcttg ggaaagaatt tgagtaatgt
     6961 tcatcaatag aattccaacg gacaggccgt ctgaaatttc agacggcctg atttaaaaaa
     7021 caatcgcttt aaggcagcga attataatat tcgtaggctt tgtccatatc ttcaaactgg
     7081 tgcatatgcc ctttttccag caccatcgca ttatcgcaat attgcttcat ggcgctgtgg
     7141 ctgtgcgaca ccaagatgat ggaacggtct ttgcgctttt caaacaactc gtacttacat
     7201 ttatcggcaa agcgcgagtc accaactgca atcacttcgt caatcaaata gcaatcaaac
     7261 tctaccgcca acgacaaagc aaatgccaaa cgcgccttca tacctgaaga ataacgcttg
     7321 accggctcgt aaagatattg ccccaactcg gaaaactctt cggtaaagtt cttcacataa
     7381 tcgatatcga cattgtaaat ccggcagatg aaacgcaaat tgtccatacc ggtcagacta
     7441 ccttgaaacg caccggagaa tgccaaaggc caagaaatac tcattgtccg cttgatttca
     7501 cccgtggtcg gcggctcgac accactgatc aaacggatga gcgtcgattt acctgcaccg
     7561 ttgcggccga gaataccgat tttctcgccc ttctccattt tgaagctaat atcgtgcaag
     7621 actgtccgcc aaccttggcg ggtcagatag cgtttggaaa cgtgttcaac tgaaatcatt
     7681 gcggctcgac tcctttactg aatttactga ccatcgccaa gccaaacaac aacaacacca
     7741 gattgcacaa tacgatatac caaggatttt cataggtaat tacatcgctg ccaaaatatc
     7801 cggcacggaa catttctgtg ccatgcacca tcggaatcat taatgcatat tcttgtacct
     7861 tgggcggcaa attatgcaca aaaaagaacg caccggataa cggcatcatc acaaaagtca
     7921 atgtgcccca aatcttgcca aacggctcga aattaaaggc aatcgaacaa atcaccaaac
     7981 ccaaaccaat cgcaaaaaaa gccatcaaaa gccaagccat cagcatataa aacatatctg
     8041 ccggcatttc aatccagcca atcgcaatca ataccgccat aatcacaatc tgcgcaatgg
     8101 ttgcaccagc aatttccaaa atcatgcgcg ccaagatggt atccaaaact cttacattgc
     8161 ggtgataaag caagctggca tttgaagaaa tcgacccaac tgcccgtttt gaggcattac
     8221 gccacatcat caacatcgga tagccagtaa tcgcaaatgc gacaatattc aaagttgaat
     8281 atcggtctgc ccttaaaaat ttccacatca agacgataac gaatgtcatc agcaacggct
     8341 caacaaacag ccataaaaag ccaatattat tgcgaccgta acgggtgata atttcccgca
     8401 tcaacagcgc accgattacg cgcctttgaa tggctaaaga ttcccaaaat gatgttttat
     8461 gcaaggcttt catcagtttt tatgctcacg aatgctggca gtcaacaggc tcaaaatacc
     8521 ataaaccatc aagccgataa tcagagtggc aacaatgttg tataaccgtt taggctcatg
     8581 tgccaaatcc ggcaggctcg gttgcgagat cacttccaaa taaagctgct gacggtctgc
     8641 ttcaaccttg gcactttcca aagaagtcat ggcggctgcc aactgctgct ctgccaactg
     8701 gttttccaaa tacacacgct gatattcggc agcctgatta gacaacgaag aatgcccacc
     8761 gcccgaaatg gcacgtaact gttggtcaat ttctttacgc aagctctgct cacgcgcctg
     8821 caaacccgga atctgcggat tctccggagt gactgctttc acctgatcca gctgggtttg
     8881 aatcacaatc aattcatctt gcagcttgga aaccaacccc atttgcactt ccgattgcgc
     8941 tttcaaatca aaaacgccat tggcaatccg gtaatccgtc agattctgag aggcttcctt
     9001 tacccgctct gccgccgttt tcactacttc ttccgcatag cgcaccgtat cagcacgtgc
     9061 acgatcgttc aactggttaa tcaatgcttc accttgtttt aacaaagcct cattgatttt
     9121 cttagattcc agcgcatcaa aggaagttac attcaacgtg gaaatacccg aaaccgtatc
     9181 aaaattgatc atcacctgat ttttatagta ttgataaaaa gcctcttcct cgccacggaa
     9241 cccaaaccca ttaaagcggc tgaacgcatc acctttggtt tcataaaact cacgcaccgg
     9301 caagatttta cgcagttcat ccaaagacga gcgcgaacgc atatactccc caaccgtgta
     9361 aatatcatct tgcgcacggg caaaacctgt gccctgcaaa atggcaccca ggccattgag
     9421 agaagattgg cttttaggcg agcgcaccac aaagctcgat tgcgacgtaa aacgatcgga
     9481 agcgaagaag ccgaaataca ccaacgaaat taccgtaggg ataatcaccg ttacccaaaa
     9541 taaagggctt agctttttaa tccaactttt ctttttcggc tttttacgct cggctttggt
     9601 ttcagtggca actgccacag gaagttgctc agacatctta attactcaca ttaattagtt
     9661 aaattattaa tactgttcgc gccactggta accggcgaga acacaaacga caaaaatttc
     9721 tgcacttcag ccaacggcgc attcgacaca tacaatacat ctttattctt cacaggaaag
     9781 cgctgcatag aaaatagcga atgcgcatca gccatattca cacgatatac cgttggaatc
     9841 tctgcctcac tgccataacc ttgagcaatc catttatcct gacgttctgc cggcaattcc
     9901 accaatggcg tatagcggaa cacaaacaca ccacgcgcat cagaacggcg atcttgcaaa
     9961 ccgcccatac ggccaatggc ttcagaaagc gataagcctc tggctgaaaa accgatttct
    10021 tgtgttctcc ccaccgcacc catagacgta aaggtatagg gattggtaat catggtaacc
    10081 acatcaccgc gacgcagcaa aatattttgt cgcggatttg caactaaatc ttccaaggca
    10141 acagttcgta ctacattgcc acgtgtcagc tgcacattcg tatcctgcac atttgccgtt
    10201 gaaccaccta ccgcagccac cgcatccaac acacgctcac cggctgccgt cagcggcata
    10261 cgcacactat tcccagcacg aatcaccgac acattcgccg cattattctg caccaaacgc
    10321 accatcactt gtggctgatt ggccattttt ttcaggcggc ctttaataat ttcctgaacc
    10381 tgaccaggcg ttttaccgac caccgaaata tcgccaacaa acggcacaga aaccgtacca
    10441 cgtgccgtga ccaactgctc tggcaactta gtttgatgcg cactacccga gcccatcgaa
    10501 gaaaggccac caccaaacaa tactgccggc ggcgcttccc aaatcataat atccaataca
    10561 tcaccaatat ttagcgtacc agccgaagca taaccatcgc caaactgagt gaatgactga
    10621 tttatctgag ccttatataa taactgagca accgtatgat tcacatcaat cagctccact
    10681 tcaggaattt gaacttcaga ttgttgccct aaagagacaa ttttttttgc gctggggcct
    10741 gatgaaggaa tcgcagagca tcctacaatt aaacttccac acaataataa tactgcgtga
    10801 cgaatataaa atttcacttt aaacacaagc caaatcctaa tataattata aatggcctaa
    10861 ttatagcact taatcgaaat aaatttatga gtacgtagag tataattagt attcttcttt
    10921 ccaacttcct tatacttata tacttatata cttatagatt ctaaaatcat gaaaagaatt
    10981 ctttgcatta caggtaccag agccgacttc ggcaagctaa aacctttatt agcctatatt
    11041 gaaaatcacc cagaccttga attgcatttg attgtaactg gtatgcatat gatgaaaaca
    11101 tatggcagaa cctacaagga agtaactcga gaaaactatc aacatacata tctgttttca
    11161 aatcaaatcc aaggtgaacc aatgggtgcc gttttaggca ataccattac gtttatctct
    11221 cgtctatctg atgaaattga acctgatatg gtcatgattc acggcgaccg tttagaagca
    11281 ctagcaggcg cagctgtagg tgcattaagc agccgtttag tttgccatat cgaaggtggt
    11341 gaactatctg gtacagtaga tgactccatt cgtcattcta ttagtaaact ttctcatatc
    11401 cacttggtag caaatgaaca agctgtcact cgcctagtgc aaatgggaga aaaaagaaag
    11461 catattcaca tcatcggctc ccccgattta gatgttatgg cctcttccac cctcccatcc
    11521 ttagaagaag tcaaagaata ttacggttta ccatacgaaa attatggtat ttctatgttt
    11581 caccccgtga ctacagaagc acatttaatg ccacaatatg cggcccaata tttcaaagca
    11641 ttagaattaa gtggccaaaa tatcattagc atctacccta ataatgacac tggcactgaa
    11701 agtattctgc aagaattatt aaaatatcaa tctgataaat ttattgcatt tccttccatt
    11761 cgatttgaat attttttagt cttattgaaa catgctaaat ttatggtcgg caactcaagt
    11821 gcaggtatta gagaagctcc tctctacggt gtcccttcaa ttgatgttgg tacacgccaa
    11881 agtaaccgcc atatgggaaa atctattatt catacagatt atgaaactaa aaatatcttt
    11941 gatgcgattc aacaagcatg cagtttaggc aaatttgaag cagatgatac ctttaatggc
    12001 ggagatactc gcaccagcac agaaagattt gctgaagtaa tcaacaatcc tgaaacgtgg
    12061 aatgtttctg ctcaaaaacg ttttatcgat ttgaatcttt aaattatgga aaaacaaaat
    12121 attgcggtta tacttgcgcg ccaaaactcc aaaggattgc cattaaaaaa tctccggaaa
    12181 atgaatggca tatcattact tggtcataca attaatgctg ctatatcatc aaagtgtttt
    12241 gaccgcataa ttgtttcgac tgatggcggg ttaattgcag aagaagctaa aaatttcggt
    12301 gtcgaagtcg tcctacgccc tgcagagctg gcctccgata cagccagctc tatttcaggt
    12361 gtaatacatg ctttagaaac aattggcagt aattccggca cagtaaccct attacaacca
    12421 accagtccat tacgcacagg ggctcatatt cgtgaagctt tttctctatt tgatgagaaa
    12481 ataaaaggat ccgttgtctc tgcatgccca atggagcatc atccactaaa aaccctgctt
    12541 caaatcaata atggcgaata tgcccccatg cgccatctaa gcgatttgga gcagcctcgc
    12601 caacaattac ctcaggcatt taggcctaat ggtgcaattt acattaatga tactgcttca
    12661 ctaattgcaa ataattgttt ttttatcgcc ccaaccaaac tttatattat gtctcatcaa
    12721 gactctatcg atattgatac tgagcttgat ttacaacagg cagaaaacat tcttaatcac
    12781 aaggaaagct aaatgcaaaa caacaacgaa tttaaaattg gtaatcgttc agtaggttac
    12841 aaccacgaac cattgattat ctgtgaaatc ggcatcaatc atgaaggctc tttaaaaaca
    12901 gcttttgaaa tggttgatgc tgcctataat gcaggcgctg aagttgttaa acatcaaaca
    12961 cacatcgttg aagacgaaat gtctgatgag gccaaacaag tcattccagg caatgcagat
    13021 gtctctattt atgaaattat ggaacgttgc gccctgaatg aagaagatga gattaaatta
    13081 aaagaatacg tagagagtaa gggtatgatt tttatcagta ctcctttctc tcgtgcagct
    13141 gctttacgat tacaacgtat ggatattcca gcatataaaa tcggctctgg cgaatgtaat
    13201 aactacccat taattaaact ggtggcctct tttggtaagc ctattattct ctctaccggc
    13261 atgaattcta ttgaaagcat caaaaagtcg gtagaaatta ttcgagaagc aggggtacct
    13321 tatgctttgc ttcactgtac caacatctac ccaacccctt acgaagatgt tcgattgggt
    13381 ggtatgaacg atttatctga agcctttcca gacgcaatca ttggcctgtc tgaccatacc
    13441 ttagataact atgcttgctt aggagcagta gctttaggcg gttcgatttt agagcgtcac
    13501 tttactgacc gcatggatcg cccaggtccg gatattgtat gctctatgaa tccggatact
    13561 tttaaagagc tcaagcaagg cgctcatgct ttaaaattgg cacgcggcgg caaaaaagac
    13621 acgattatcg cgggagaaaa gccaactaaa gatttcgcct ttgcatctgt cgtagcagat
    13681 aaagacatta aaaaaggaga actgttgtcc ggagataacc tatgggttaa acgcccaggc
    13741 aatggagact tcagcgtcaa cgaatatgaa acattatttg gtaaggtcgc tgcttgcaat
    13801 attcgcaaag gtgctcaaat caaaaaaact gatattagtt aataataaaa tagattaagc
    13861 tattcttaaa ttcagaatat tgcttatcta tattaaaaat ttctaatttt taaggttctg
    13921 attgaaatca gaaccttatt tcaactatta ctttttactc ataatcgaat tatatacttt
    13981 aggactttat aatatggctg ttattatatt tgttaacgga attcgggctg taaatggcct
    14041 tgttaaatca tctatcaata ctgcaaacgc ttttgctgaa gaaggactgg atgttcattt
    14101 aattaatttt gttggcaata ttactggagc agagcattta tcccccccat tccacttaca
    14161 tcccaatgtc aaaacctcca gcatcataga tttatttaat gacattccag aaaatgttag
    14221 ctgccgaaat attccttttt attctatcca tcaacaattc ttcaaagccg aatacagtgc
    14281 ccactataag catgttttga tgaaaattga atctttatta tctgaagaag atagcattat
    14341 cttcactcat cctcttcaac tggaaatgta tcgtttagcg aataataata ttaagtcaaa
    14401 agccaagcta attgtacaaa ttcatggtaa ctatatggaa gaaatccata actatgaaat
    14461 tttggcacga aatatcgatt atgttgatta tcttcaaacg gtatctgatg aaatgctgga
    14521 agaaatgcat tcccatttca aaatcaaaaa agacaaatta gtttttattc caaacatcac
    14581 ttatcccatt tcattagaaa aaaaagaagc tgatttcttt attaaggata atgaagacat
    14641 tgataatgct cagaaattta aacgtatctc tattgttggc agtattcagc caagaaaaaa
    14701 ccaattggat gccattaaaa tcatcaataa aattaaaaat gaaaattaca ttttacagat
    14761 atatggcaaa tctattaata aagattactt tgaattaatt aaaaaatata ttaaagacaa
    14821 taagttacaa aaccgtatct tattcaaagg tgaatcttcc gagcaggaaa tttatgagaa
    14881 tacagatatc ctaatcatga catctcaaag cgaaggcttt ggttatatat ttctagaggg
    14941 tatggtgtac gatatcccta tccttgccta taattttaaa tatggagcga atgattttag
    15001 caattataat gaaaacgctt cagtttttaa aactggtgat atttctggaa tggcaaaaaa
    15061 aataattgag ctattaaata acccagaaaa atataaagaa ttagttcaat ataatcacaa
    15121 tcgcttctta aaagaatatg caaaagatgt ggttatggct aaatatttca ctattcttcc
    15181 gcgcagcttt aataacgtat cattatcgtc tgctttcagc cgaaaagaat tggacgaatt
    15241 ccaaaatatt actttttcta ttgaagattc taatgattta gctcatattt ggaatttcga
    15301 gctaaccaat cctgcacaaa atatgaattt ttttgcttta gttggcaagc gaaaatttcc
    15361 aatggatgct catatccaag gaacacagtg tacgattaag atagctcata aaaagacagg
    15421 gaatttattg tcgcttttac taaaaaaacg aaatcagttg aatttatcaa ggggatatac
    15481 cttaattgca gaagataata gctatgaaaa atatattgga gcaatatcta ataaaggtaa
    15541 ctttgaaatt attgcaaata aaaagaactc attagttact ataaacaaaa gtaccttaga
    15601 gttgcatgag attccccatg aactacatca gaataaatta ctgattgctt tacccaacat
    15661 gcaaacgcct ctaaaaatta ctgatgataa tttaatacct atccaagcct ccataaaatt
    15721 agaaaagatt ggaaatactt attacccatg tttcttgcca tctggcatat ttaataatat
    15781 ctgcttagat tacggtgaag aatccaaaat tattaatttt agtaaatatt cttataaata
    15841 tatctatgac tcaattcgtc atattgagca acatacagat atatcggata ttatcgtttg
    15901 caatgtttat tcttgggaac ttattcgtgc ctcagttatt gagagcctta tggaatttac
    15961 cggaaaatgg gaaaaacact ttcagacttc tcctaaaatt gattatcgat ttgatcatga
    16021 aggtaagcgt tcgatggatg atgtcttttc agaagaaaca tttattatgg aatttccgcg
    16081 taaaaatggt atagataaga aaacagcagc cttccaaaat ataccaaaca gtattgtaat
    16141 ggagtatccg cagaccaatg gttacagtat gcgcagtcat tcactgaaaa gtaatgtagt
    16201 tgcggcaaaa cattttcttg aaaaattaaa taaaattaag gtagatatta aatttaaaaa
    16261 gcatgacctt gcaaacatca aaaaaatgaa tcgaattatt tatgagcatt taggcattaa
    16321 cataaatatc gaagcatttc taaaaccacg attagaaaaa tttaagcgtg aagaaaaata
    16381 ttttcatgat ttcttcaaaa gaaataattt taaagaggta atttttccaa gcacttattg
    16441 gaatccaggt attatttgtg ctgcacataa acaaggtatt aaggtatctg atattcaata
    16501 tgctgccatt actccttatc atcctgcgta ttttaaatca ccaaaatcac attacgttgc
    16561 tgataaattg ttcttatggt ctgaatattg gaatcatgag cttttaccaa atccaacacg
    16621 agagattggt tctggtgccg catattggta tgcattagat gatgtgagat tttcagaaaa
    16681 actgaattat gactatatct ttctatctca aagtaggatt tcttcgcgct tgcttagttt
    16741 tgcaattgag tttgcattaa aaaatcctca actacagctt ttattttcta agcatccaga
    16801 tgaaaatata gatttaaaga acagaattat tcctgataat cttataatct ccacggaatc
    16861 ttctatacaa ggcatcaatg aatctcgcgt tgctgtaggt gtttattcaa ctagcttatt
    16921 tgaggcatta gcatgcggca aacaaacttt tgttgttaaa tatccgggat atgaaattat
    16981 gtcaaatgaa atagattcag ggttattctt tgcagtagaa acacctgaag aaatgcttga
    17041 gaaaacaagc ccgaattggg tggctgtggc agatattgaa aaccagtttt ttggccaaga
    17101 aaaataattt ttaagttaat ctttaaaagt gcaatatttt tcttatttta aatattgcac
    17161 tttattcttt taatcatagg aactcacatg tattctgaac agggaattaa taatactatt
    17221 aacatatcta ctacatcttt aaccaatgcc acgcaactaa ctgtgatcgg gaataataac
    17281 tctgtatata taggtaataa ttgtaaaatc gtcagctcca atattagatt aaaaggaaat
    17341 aatataacct tatttatagc tgatgatgta gaaatcatgg gactagtatg ttcccttcat
    17401 tcggactgct ctttacaaat tcaagccaag accactatgg gcaatggaga aattaccatt
    17461 gctgaaaaag gtaagattag tattggcaaa gattgtatgc tggcacatgg atatgaaatt
    17521 agaaatactg atatgcatcc aatttattct ttagaaaatg gcgaacgaat aaaccatggt
    17581 aaagacgtta ttatcggtaa tcacgtgtgg cttggccgca atgttactat attaaaaggt
    17641 gtatgtattc caaataatgt agttgtaggt tctcatactg tgctatataa aagttttaaa
    17701 gaacctaatt gtgtaatagc aggttcacct gcaaaaatag taaaagaaaa tattgtatgg
    17761 ggaagaaaaa tgtatcattc aactatgtat gatgacccta ctttaaatga attttataaa
    17821 taa
//
LOCUS       DAWHXZ010000016        17735 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000016 DAWHXZ010000000
VERSION     DAWHXZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17735
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.016"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..408)
                     /locus_tag="WIE35_000363"
     CDS             complement(<1..408)
                     /locus_tag="WIE35_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153334.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWK"
     gene            complement(431..1909)
                     /locus_tag="WIE35_000364"
     CDS             complement(431..1909)
                     /locus_tag="WIE35_000364"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ4153335.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDNVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(1934..3679)
                     /locus_tag="WIE35_000365"
     CDS             complement(1934..3679)
                     /locus_tag="WIE35_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ4153336.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(3740..4003)
                     /gene="ftsL"
                     /locus_tag="WIE35_000366"
     CDS             complement(3740..4003)
                     /gene="ftsL"
                     /locus_tag="WIE35_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ4153337.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(4000..4986)
                     /gene="rsmH"
                     /locus_tag="WIE35_000367"
     CDS             complement(4000..4986)
                     /gene="rsmH"
                     /locus_tag="WIE35_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ4153338.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGSIPEQSQRKTSEWQ"
     gene            complement(4983..5438)
                     /gene="mraZ"
                     /locus_tag="WIE35_000368"
     CDS             complement(4983..5438)
                     /gene="mraZ"
                     /locus_tag="WIE35_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ4153339.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            5717..6934
                     /locus_tag="WIE35_000369"
     CDS             5717..6934
                     /locus_tag="WIE35_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ4153340.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGVD
                     WAGFRFGDICHKL"
     gene            7066..7142
                     /locus_tag="WIE35_000370"
     tRNA            7066..7142
                     /locus_tag="WIE35_000370"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7100..7102,aa:Met,seq:cat)
     gene            7150..7225
                     /locus_tag="WIE35_000371"
     tRNA            7150..7225
                     /locus_tag="WIE35_000371"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Ala,seq:ggc)
     gene            complement(7507..8328)
                     /locus_tag="WIE35_000372"
     CDS             complement(7507..8328)
                     /locus_tag="WIE35_000372"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ4153341.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(8361..9005)
                     /locus_tag="WIE35_000373"
     CDS             complement(8361..9005)
                     /locus_tag="WIE35_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ4153342.1"
                     /translation="MKLKHLLPLLLSAALSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYRIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(9008..9871)
                     /gene="ftsN"
                     /locus_tag="WIE35_000374"
     CDS             complement(9008..9871)
                     /gene="ftsN"
                     /locus_tag="WIE35_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ4153343.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPV
                     PSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAAKEAEQSDAEKAADKQPVADKA
                     DEVEEKADEPEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            complement(9977..11473)
                     /locus_tag="WIE35_000375"
     CDS             complement(9977..11473)
                     /locus_tag="WIE35_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ4153344.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSHRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(11487..11807)
                     /locus_tag="WIE35_000376"
     CDS             complement(11487..11807)
                     /locus_tag="WIE35_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ4153345.1"
                     /translation="MFGKQLFEEVGSKISETIVNSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            12291..13817
                     /gene="putP"
                     /locus_tag="WIE35_000377"
     CDS             12291..13817
                     /gene="putP"
                     /locus_tag="WIE35_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ4153346.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIVYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            14068..17673
                     /gene="putA"
                     /locus_tag="WIE35_000378"
     CDS             14068..17673
                     /gene="putA"
                     /locus_tag="WIE35_000378"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ4153347.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWVHG
                     GSLLQAFCALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4134 a         5138 c         4379 g         4084 t
ORIGIN      
        1 tttccaaact gtcaagccaa aaatgaaaaa taatgcctgc acaaaataaa gcgacccggg
       61 aagattgccg acaaattcac gcgcctcggc aggattgctt tccaatatcg aaccgactat
      121 ctgataagac ggcgcaccat acaaccagcc gaccggcaaa tatagggcgg ttgtgccgac
      181 gtaaatcagt aaaacaactg aagaaacgcg cgggaaaccg cgtgccaata ataataaaaa
      241 tacaaccgaa gccaaagcac ccacggcaga ataacggtag ccgtattcat attccaagtg
      301 ataacctgat gcgatggccg cgccaaaaca aaaggctaaa agcgcgcaaa tgcccaatgt
      361 attttttaat gtctgatcca tattttttat tcgggcggat tgtttcatac gatgccgtcc
      421 gaatatccca ttatttacga gttaacaaag cctgtccgac gatttcaaga tcggaaaaac
      481 ggtgcttcac gccttgtaca tcctgatagt tttcatgccc tttgccggca atcaggatga
      541 tgtcgtttgc ggcggcttgt tcaaccgcat aacggatggc ggcggcacgg tcggcttcga
      601 cgcattcggg cgcgggaacg gcaggcagga tgtcgttgat gatgtcgtgc ggattttcca
      661 aacgcggatt gtcgctggtg acgacgactt tatccgcgcc ctgtacggct gccgcgccca
      721 tcagcgggcg tttgccgcga tcgcggttgc cgccgcaacc gaatacgcac cacaaagccg
      781 caccctgcgg tttgatttcc tgcaaggtgg cgagtgcttt ttccaatgcg tcgggcgtat
      841 gggcataatc gacaacgacc aaaggtttgc cgctgttcat gatgcagtcc atgcgccctg
      901 aagcgggacg gatttttgcc agcacatcca atacattatc aagcggatag ccgttggcgc
      961 acagcaaggc gatgcaggcg gcgaggtttt gcgcgttgaa ccgtccgagc aggcgcgtgc
     1021 ggcatttccc ttcgccccac ggggtttgga atacagctgc tatgccgtct gaagaggcgg
     1081 tgaaatcgat aatgcggatg tcggcgtgtt cgctgaagcc gtagctataa acggccaaat
     1141 cgggacagtc ttttttcaga cgacctacga gttccgcgcc gtattcgtca tccacgttga
     1201 tgactgcgtg tttcaagccg tgccagtaaa acaggcgcga cttgatggca ccgtaggctt
     1261 ccatcgtgcc gtggtagtcg aggtggtcgc gggtgaggtt ggtaaagatt gcgctgcgga
     1321 atgacacgcc gttgacgcgc gactggtcaa gcccgtggct ggagacttcc atcgcggcga
     1381 ctgttgcgcc ttgttgacgg aaacggtaga gcagggtttg gacatcgacg ggggcgggtg
     1441 tggtatgcgt ggtttcttcc aatgcacccc aaaagccgtt gccgaccgtg ccgacaatgg
     1501 cggttttttc gcccaacaaa tcggcagctt gcgccagcca ttgcgtgatg gaggttttgc
     1561 cgttggttcc ggttacgccc cagaatttga gaccgtctga aacgttgccg taaacttgcg
     1621 ccgccaatat gccggcacgg tgtttcaaat ctttgatgcc ttgattgggg actttccatt
     1681 cgggattcca cgcaaatttg ccgtcgtcgt cccaaaaaac aaaagccgcg ccgttggcaa
     1741 cggcggcggg gatataactg cgtccgtcgg catattcgcc cggacaggca acgaaaatat
     1801 cgccttgttt gatttggcgg ctgtctgaat gcaacaaacg ccctgccgcg tttgcacacg
     1861 acagggtcgg gatgccggtt tcagccaaag gggttaactt gctgaacata aaacaatctc
     1921 gttgatactc ggattaagac ggtgttttga cggctgcggc ggtcaggggc ttggtcgggg
     1981 aaatgcccaa gatgttcagg ctgccgccca taattttttt gaagggcggc cctgccacta
     2041 cgccgccgta ataaccgtgg gcagtcggtt cgtcaatggt taccgccaca atcacacggg
     2101 ggtttttggc gggggcaaaa ccgataaagg tagcgatgtg tttgttgtcg gcataacgcc
     2161 cgttgacgaa cttgcgcgcc gtgccggttt tcgcgccgac atcgaaaccg tccaccgcac
     2221 ccgccgtacc ggtgccgccc ggctcggtca cggaaaccat cagattgcgt acctcgcgcg
     2281 cggtcgattc tttgaatatg cgtttgcctt gcggcgcaac cgcctgtttt tcaaagctga
     2341 ccggcagcaa aacgccgtcg tgcgtcagtg cggtataggc gcgcgccaat tgcagcaggc
     2401 tcaattgcag gccgtaaccg aaagacatcg tcgcctgttc aataggccgc cacctgcgcc
     2461 aatttctcaa caaacctgca gtttcgcccg gaaagcccga gtgcatacgc acaccgatgc
     2521 ccaactcatg atagaagtca tacatttctt cggcaccgaa acgcgcagac agtttgcttg
     2581 tgccgacgtt ggacgatttc tgcatgatgc cgcgcacatc caaagagggg taaacatggg
     2641 tatcgcgcac gggagacggt ccgattttat aaggctgcgt attcagccgt tcgttcaaat
     2701 cggttttgcc cgcatccaat gctttcgcaa tcacaaacgg tttgattgcc gaaccgggtt
     2761 cgatcatatc ggttacggca cggttgcgcc gctgttcgct gtctgcccgg ccgggcctgt
     2821 tgggatcgta ggcgggcgta ttggccaagg cgaggatttc ccccgtgcgg gcatccaaaa
     2881 ccaccaccgt tccggctttt gcctgatggt attcgaccgc cttgttcaac tcttcatagg
     2941 ccaaggtctg aatcctctga tcgagggaaa ggatgatgtc tttgccgttt ttcggggctt
     3001 tattgcgcgg ggagtccaag ctgtccacaa tattgccctg ccggtcccgc aaaacgactt
     3061 ccgcgccgtc ttcgccatgc aggctgtctt caagcgaaag ttccaaacct tcctgacctt
     3121 tgccgtcaat atcggtaaat ccgatgacgt gtgcaaacag gttgcccatc gggtaatggc
     3181 gttttaattc tttttcaaat acaaagtttt ccaaacccaa ggctttgacc tcttcggcaa
     3241 ccttgggatc gagctgccgc ttaatccaga taaacgactt gcctttctgt tcgagcttgt
     3301 tcctcaaaac atcaaccggc acatcgacaa gctcggacag gcgttccaat tgtgcggcag
     3361 acggcatttc cttcatctct ttaggcacgg caaacaggga ctccgtcggc gcactcaacg
     3421 ccaaaaccgc accgttccgg tccgaaaccg taccgcgtgt agccggcaat gtttgagtcc
     3481 gcacaatccg gttgtcgccc tgttctttca aaaagttata cgttaccgtc tgcagataca
     3541 gtccgcgagc aatcagaccg gcaaacaaga ccgctattgc catcaggacg aagctgatcc
     3601 gtccgttact ggtcatcggc tttttgacct gctcttcttt gggcagcatc cgaggcttat
     3661 attcgctctt aatcaacatt tttacttctc gttattatta tcctgacgca ggaatccgat
     3721 tccggcacac aggctgcttc tatctttgat gcgccaccat aaaggtattg cccgaaaccg
     3781 gcggatggag gttttgtttt tctgccgccg ccctgatcgc ttcgtggttc gccaaacgcg
     3841 cctgttgcag cctcatttgc gcataatcct gctccaaggc gatttcctgt tttttcgcct
     3901 tgtccaaagc tgtgaagttg agcctgtact ggttttgctg catcacaacg gaaaaagcgg
     3961 aaacgcacac cgcaagcagc agaaggaaat tcaatttgtt cattgccatt cagacgtttt
     4021 tctctgtgat tgttccggta tcgaaccggc agtccgctcc gccacacgca aaaccgcact
     4081 tctcgccctc ggattggcgg caatttccgc ctcacccggc tttaatgccc tgcccacgat
     4141 tttcaggggc ggctcgggca aatccgcttc cctgaccgcc gcccagcgcg gcaggggcgc
     4201 gtgttgcgaa tattttttga caaactgctt cacaatgcgg tcttccaacg aatggaaagc
     4261 aatgaccgcc aaacgtccgc cctctttcag acgacacatg acctgcggca atactgcccc
     4321 tacttcttca agctcgcggt taataaagat gcggaccgcc tggaaggtgc gcgtcgcagg
     4381 atcctgcccc cgctcgcgag tacggacgtt ttgtgccacg atctgcgcca gcttgcgggt
     4441 tgtatcgatt ggactttccg cccgttgcgc aacaatggcg cgcgcaatct ggcggctaaa
     4501 ccgctcttca ccataattct tgattacctc gtgcaaatcc tgttccgacg caaccgctat
     4561 ccactctgcg gcagacatac cgcgcgtcgt atccatacgc atatccaaag gggcatcgaa
     4621 acggaagctg aaaccgcggc tgccgtcatc gatttgcggg gacgaaatcc ccaaatcaaa
     4681 cagcgcaccg tccaccttgc cgatacccaa accgtccaat gccgtctgaa acgaagcaaa
     4741 accgccatgc acgacaccga cccgtttgtc cgaacgcgcc agctcttctg ccacagcaat
     4801 cgcctgaggg tctttgtcga aaacaatcaa ccgccccgca tcgcccaaac gcgacaaaat
     4861 cagccgggaa tgccctcccc tgccgaacgt accgtccaca tagacaccgt cttcgcgcac
     4921 ggcaagcgca tccaccgcct cattcagcaa gaccgtgata tgccggtaac tttctgctcc
     4981 actcacaatt gcaaatccgt ctgacccaac tggaaggcaa gttcgtcagg atcgtcatcc
     5041 aaagcctgaa ccatctcagc ctcccactgc tcgcgacccc aaagctccaa acggttggca
     5101 cgaccgacca aaacgacttc acggtcgaaa tccacccttt tcctcagtcc ggcaggaagc
     5161 agcacccggc cggcgctgtc ccattccaaa atttccgcgt tatgcagcaa aagattttga
     5221 aaccgccgca aaacagggtt atccgccact tttaagttta aaagttgcgc cgcaaccttt
     5281 tcccactccg caacagggta catcaacagc ttgtgtttcg actcgagcgt taccactacg
     5341 gcaggcgtat agaggcgcga cagaatgtca cggaatttgg caggaacagc caaccgcccc
     5401 ttactgtcga tgcttaattc gtgtgcgccg ccgaacatga catgtcccaa gccgaaatca
     5461 aaatcacaag ggtaaaagag acactttgcc ccacaattcc ccaccaatcg acactataag
     5521 aaattttaaa cactcggtca aatcagggca tgaaaactca ttaacatatc tgaaattttt
     5581 attcctttta aaaacaataa gataaaaaat gacgacaacg gcacggcggg tgcggtacag
     5641 aataatcgaa ccaataaaca actatatatg attaatttaa taatataaac acaatatata
     5701 gtattaagat aaagccatga cagcacccgt accaacgtgt aatatgtcgg gaaatccaat
     5761 aaatttacac aagctaacac ttatcatgcc cctccccctc ccctctcccg aagcacggca
     5821 attctcgctc aaactgcaaa ccctcattgc cgaaaaaatc ggcaaacacg gcaactggat
     5881 tccattttca cgttttatgg aattggtttt atacgctccg caatacggct actacaccgg
     5941 cggcagccat aaaatcggca ataccgggga ttttattact gcaccgaccc tcacctctct
     6001 gtttgcacag acactggcac gccaacttca agaacttcta tcccaaacgg cgggcaatat
     6061 ctatgaattc ggcgcgggaa ccggacagct tgccgccgat ttgttgggca gcatttcgga
     6121 cggcatcagc cgttactata ttattgaaat atcgccggag ctggcagcac gtcagaaaaa
     6181 cctgattcaa gcccgcgcac cggaagcatc tcaaaaagtt gtccacttga ccgcacttcc
     6241 cgaagcgttt gacggcatca tcatcggcaa cgaagtactc gatgccatgc ctgtcgaaat
     6301 cgtccgtaaa aatgaaggcg gctcattcga gcatgtcggc gtttgcctag ataatgaccg
     6361 ttttacctat tcggcacgac cgctgcacga cttacagcta tctgccttgg cttccctcta
     6421 ttttcctcaa acagattatc cctacaccag cgaactacat ccgcaacaat atgcctttat
     6481 ccgcaccctt gcctcaagac ttgaacgcgg gggtatgata ttcatcgact acggttttga
     6541 tgcagcgcag tattaccacc ctcaacgcaa tcaaggtact ctgatcggac actaccgaca
     6601 tcacgttatc cacaatcctt ttgacttcat cgggttggcc gacctgaccg cgcatgtcaa
     6661 ctttaccgac attgcccaag ccggtacgga tgccggatta gatttgatag gttaccttcc
     6721 ccaatcccat ttcttattga acctgggcat taccgagcta ttggcacaga cggggaaaac
     6781 ggattcggca gcctatatcc gtgaagctgc tgccgttcag aaactgattg accagcatga
     6841 aatgggcgaa ctgtttaaag tcatcgcatt cggaaaaaat atcggcgtcg actgggcagg
     6901 attccgcttc ggcgacatct gccacaaact ctaaccctca tgccgcctga atccgcttca
     6961 gacgacataa actttttaac atttaaaaac agtcaactaa ttcaaaatta aaaaatacgg
     7021 cttgtcaaaa aaacagaaaa acatataata gcgtcttcac gaaacggcga attagctcag
     7081 tcggttagag cagaggaatc ataatccttg tgtccggggt tcgagtccct gattcgccac
     7141 caaattttcg ggggtatagc tcagttggta gagcgcttgc atggcatgca agaggtcagc
     7201 ggttcgattc cgcttacctc caccagataa aaaagcacag accgtaaaaa ggtatgtgct
     7261 tttttattgc ctgattgcca gcaaataaag aataaaccac tgccttcaaa acaggcaatc
     7321 gactttaaac ctatcgcccc gcctgtcctg attttatagt gaattaaatt taaaccggta
     7381 cagcgttggc tcgccttgcc gtactatctg cggcttcgtc gccttgtcct gatttttgtt
     7441 aattcactat atcagcccgc cagacaaacc cgacccgaat aatgtcttca ggtcgggttt
     7501 atggtttcat tcccaactta tccagcctga cagccacaat ataatgatgg caataccaaa
     7561 aacaatgcgg taataggcaa aaggaatata atttttcttg gaaacaaacc tcagcaacgc
     7621 ttttaccgct accaagcctg aaacaaaggc agcaataaag cctatcagaa tcaaaccgac
     7681 atcatgcagg gtgaaaaatc ggtaatgttt caggacatca taagccgttg cggcaaccat
     7741 catcggcaca gccaagaaaa acgagaattc tgtcgcagtt ttccgttcga tgccccaaag
     7801 catcccgccc ataatcgtac tgcccgaacg ggacgtaccc ggaaccagtg caaacacttg
     7861 ggcaacgccg atcatcaagg catcaatcgg acgcaatgca tcaacatcgg caattttagg
     7921 ctctgctcgg ctttggcgtt tctccaccca caaaataaaa aaaccgccca aaaccagcat
     7981 gactgcaaca ctcaaggggt taaacagata ctctttgatt tgtttgccga acaacagccc
     8041 catcacggcg gcaggtataa aagcaatggc aagattaagg acgaagcggt tggctttccg
     8101 gtcttttccc aagccgtgca acacattgct gaaacgttgc cggtattcaa acactaccgc
     8161 caaaaccgca ccgagctgga tggcaatttc aaaaaccttg tgattgctgt gaaaaccaat
     8221 cagattgccg aacacaatca aatgtccggt gctggaaatc ggtaaaaatt cggtaaaacc
     8281 ttctaccaag cccatcatca gggctttcag gacaatcaga aaatccattg cttgcgcttc
     8341 tttcggatac gggagttcgg ctatttctgt acagcagggg tctgacgctt gcgttcttcc
     8401 ctgactttgg caaccaattc tttaatcgta tgaacgccgc cgtcaaagcc gttattgaag
     8461 ataacgcggt attttccgcc gacaataacg gtcggcgtgc tgtcgatgcg gtattgttcc
     8521 gtcagtttct gcatttttaa tgcggcggcg gcagcttcgg gggaatcata ggcgcgcatc
     8581 agttttttgc cgtcaaagcc tttttgagac aaagcccatt ttccggcaac agccctgttt
     8641 tccaagcgga ttttttgttc gtaaactgct ttaaacacgg cagggtttgc ctgatatttc
     8701 aaacccgaca aattgacggc agccgccatc ctggccagac cgagcatttc aggctgccag
     8761 accacgtgct ccgtcctcaa ataggcatca gacggcaatg ccttgcccag tttcaataac
     8821 aaaggatcga aatgatggca atgtacgcag aaatagccga aaaattccaa aacctcaatt
     8881 ttacccgact gttcttgagg aatgggttta tccaacacaa gatagtcttc cccttccgtc
     8941 agggcatatg cctgcgcgga caacgctgcc gacagcagca gcggcaacag atgtttgagc
     9001 ttcataatta tttactttcg atagaacgga tcaggctggc gacttcatgt tttttcaact
     9061 cgtcctgcat ttttttcacc gcatcggcag acatattgcc gctttgcacc cggtaaagcg
     9121 ttttatgtcc cgcctgataa ccgaccacct tggaagatat gcccaagatt gccagtttgg
     9181 cacgctgtcc ttccgcgctc cggcggtcgg catacgcgcc catttgcaga taatgcgttg
     9241 cttccgcctt gtcgggcgtt ttcattttct gcacttcttt ggcagcggca ctgcgcgctt
     9301 tttcgatgct gccgctgttg aggatttgtt ccggggtcgg tttgggtgca actttttcct
     9361 tctccgcctt tttctcttct tttgaagctt ttttctctgt ttctttagat ggttttaccg
     9421 cttgtttttt aaccgtttcg gcatctttct tctgcgcttt ttccccgacg gtttgttcac
     9481 gctcttccgt cagtgctttc ttgcgcactg cctgtccgtc cgacttttcc cgctccggct
     9541 cgtccgcctt ttcctcaacc tcgtcggctt tgtcggcaac gggctgcttg tcggcagctt
     9601 tttccgcatc cgactgctct gcctctttcg cagcatccgg ttcggacaag gcgttttgat
     9661 cggccggttc aggttggatg tcttccttag gctggttttt cggtttcagg atttccgttt
     9721 ctgcaggctg cttcgacgga accgggattt tgaacgcatt ttgaccgctc tggttcagat
     9781 aaaacaaaat accggcaata atgaccgtcg ccagtatcaa accgaagaaa aaaccggaca
     9841 gaccttttcc ggattgggaa aatttgttca taaacatacc ttaatgtgtt tcagacggca
     9901 ttaacgccgt ttgcttacgg gcggatattc taacaatatc gccatatttg ggcaaaacct
     9961 gcttccattc ccattcctat aaagcacgac ggaaacctat ggctttcatg acgtggcttc
    10021 tgccgacttc ttcgtcgccc gccaaatccg ccaatgtacg cgccacgcgc ataatgcggt
    10081 ggaagctgcg ggcggaaagg gagagttttt ccagcaggcc gcccaatgct tcctgcgctt
    10141 ctttttgaat gcgggcttgg gaatcgagtt cgctgacact caaagcggcg ttgactttgc
    10201 cctgccgcgc gtattgtttg tcgcgggcgg cgataacgcg ttccaaaacg gacgcgctgc
    10261 tttcacccgc ttcctgctgc atcagttcgg cggcggacag gctggggact tcgatggtca
    10321 aatcgatgcg gtcgagcagc gggccggaaa ttttgctgcg gtaacgcgcg acgctttcgg
    10381 gcgtgcagcg gcagggtttg acgggatgcc cgagataacc gcacgggcag gggttcatgg
    10441 cggcaacaag ttggaatttg gcaggataga cggcttggcg cgccgcgcgg gaaatgtgga
    10501 tttcgccgtt ttccaacggt tcgcgcaaaa cttccaaaac tttgcggtcg aactcgggca
    10561 gctcgtccaa aaacaatacg ccgtggtgcg ccaatgaaat ctcgcccgga cgcgggtcgg
    10621 aaccgccgcc gaccatagcc gccgcgctgg cactgtgatg cggactgcgg aaaggacggt
    10681 ggctgtcgag ttgttgctgg tggttgggca ggagcgaacg caatgcccaa acttcaacca
    10741 attcgtcttc ggtcagcggc ggcaggatgc ctggcagccg ttgggagagc atagacttgc
    10801 ccgttcccgg cggacccatc atcaagaggc tgtgtccgcc tgcggcagcg atttccaaag
    10861 caaggcgggc agtgtgctga cctttcacat cacacaaatc aggttgtccg ccatgttcag
    10921 acggcatttg cggtacggaa cattcggttt gcgccaaagg ttcgataccg ttcaaatggg
    10981 cggcgacttc gcccaaagag cgcgcgccgt aaacggtaat gccgcgcatc acggcggctt
    11041 gtcctgcgtt ttcttcaggc aaaacaaatg cacgttttgc ctgcataccc tgccacgcca
    11101 tcgccaacgc gccgcgcacg gggcgcaaca gccccgacag tgccaattcc cccgcaaact
    11161 cgtattccgc cagtttttcg ggcgcaacct gccccgatgc ggcaaggatg ccgattgcaa
    11221 tcggcaaatc gaaacgcccc gactctttgg gcaggtcggc gggggcgagg ttgacggtga
    11281 tttttttggc ggggaattca aaaccgcttt gaataatggc ggcacggacg cggtcgcgac
    11341 tttctttgac ttccgtatcg ggcagtccga cgatgttgaa atgtggcagg ccgttggcaa
    11401 ggtgggcttc cacttcgacc aacggcgcat tcataccgct caaggcgcgg ctgtaaacca
    11461 aggcaagcga catatttcag acggccttat tcgccggctt cggtttgctg cctgatttcg
    11521 gcgacggctt cttcggcagc ggcttcagcc gcttccaatg ctgcccgttc gggattttgc
    11581 gcggcttcga gttttgccaa acgcgcttcc aaagccgcca gtttggtacg ggttttgatt
    11641 aaaacctgct gctggatgtc gaattcttcg cgcgtaacca aatccatacg gttgaacgcg
    11701 ccgcccagca tcgccttaat atttttttcc acatctttgg cagggctgtt gacgatggtt
    11761 tcgctgattt tcgaaccgac ttcttcaaaa agctgcttgc cgaacataat ctgtatcctt
    11821 cctgaacata tcaaattcaa tcggctattg tataaggaaa aatgccgtct gaaaacgggc
    11881 ggcggataat cggcaaaaca taccgcgcct cctttgcggt tgctaaatta cacctcaaaa
    11941 cacctgccgc ctgaaaacgg atttcatatt ccgcgcaacg ccagattaca agacactaca
    12001 aaacaatata ctgttttaaa tgatttttcc gacgcgcatc gtttcaaact cggcttttta
    12061 agccattaag tgctttgcaa acaacagaaa ttgggttatg ctgaaacgga ttatttacaa
    12121 tttcatatag ttttattaca tatcttattg tgattgaaga taatttatcc gaatccccct
    12181 ttcgggtatc cggattttcc gttgtacttt tatttagaaa aacatttcag gcgcaagttg
    12241 cttgcaattt caaagcctgt aattctgaag ttttagacga ggaatagcac atgaatccca
    12301 tgtacattac ttttgcaatc tatctggttg cagtccttct catcgggctt gccgcctatt
    12361 tttctacgcg caatttcgac gattatattt tgggcgggcg cagcctaggc ccgtttgtta
    12421 ccgcgatgtc ggcaggcgcg tccgatatgt ccggctggct gctgatgggt ctgccgggcg
    12481 cgatttacct gagcggtttg aatgaggctt ggattgccat cggcctcttg gtcggcgcgt
    12541 atttcaactg gctcttggtg gcgggccgcc tgcgcgtgca taccgaatac gccaacaatg
    12601 cgctgacgct gccggattat ttcttccacc gcttcggcgc gggcggacac ttgatgaaag
    12661 tggtttccgc actaattatc ttgtttttct tcacaattta ctgtgcttcc ggcattgtgg
    12721 ccggcgcaac cctgttccaa agtctgtttg aaggtatgac ttacaaccag gcaatgtggc
    12781 tgggtgcggg cgcgaccatc gtctatacct tcttgggcgg tttcttggca gtcagctgga
    12841 cggatacgct gcaagcatct ttgatgattt tcgccctgat tttaacgccg gtgatggtct
    12901 atctgggctt gggcggcgcg gaacagatgt ctgccgcgat tcaaagcgtc gccgcaggca
    12961 cgggcaaaga atacggcagc ctgtttgccg gtacgaccgt catcggcatc atttccaccg
    13021 ccgcatgggg cttgggctat ttcggacagc cgcacatttt ggcgcgcttt atggcggccg
    13081 aaagcgcgaa atcgctggta tccgcacgcc gcatcggcat gacttggatg gcgttgtgcc
    13141 ttgcgggcgc ggtagcggtc ggttatttcg gcattgcgta tttcggtgca aatcccgaca
    13201 aagtttcttc tatgagcggc aaccacgaac gcatcttcat cgcgctttcc accctgctgt
    13261 tcaacccttg gatcgccggc attattttga gcgcgatttt ggcggcggtg atgtccaccc
    13321 tgtcctgcca gcttttggtt tgctccagcg cgattaccga agacttctac aaaggctttt
    13381 tgcgtaaaaa cgcgcaacag tcggaactgg tatgggtcgg ccgcctgatg gtcttggcga
    13441 ttgccgtgat ttccatcctg attgcttccg atccgaacag caaagtattg ggactggtgt
    13501 cttacgcgtg ggcaggtttc ggtgcggcat tcggcccgat tgtgattctg tccgtattgt
    13561 ggaaacgcat cactgcctac ggcgcgttgt cgggtatggt tgcaggtgcc ttaaccgtgg
    13621 tcgtatgggc ggaatgggtg aaaaaaccgg ctcaagcggc aggcgaaagc ggcttgttga
    13681 cgatgtacga aatcgtcccg ggctttattg tttgcttgat cgttgccgtg ttggtttccc
    13741 tgttcaacaa agaaccttcg cgcgaaaccc aagaacgctt tgaaaaagcc gacgcagatt
    13801 accgcgccgc ccgataaacc cctttcagac ggcacggcat cccggttcgt gccgtctgaa
    13861 cccgtttccg ggtcaaacac aaataaacct tgcctgcata caggccttat ttgtgtttta
    13921 cccgtttcat tttcaaagaa acggaagcac ctaccctgcc cgcatttgaa gccttgtatt
    13981 tcatctgcgg atttattacg atattaccgt attcagaccg caccgatgcc gtctgaaccc
    14041 cccgaaaaac tttggagaat ccaaaaaatg tttcattttg catttccggc acaaactgcc
    14101 ctgcgccaag cgataaccga tgcctaccgc cgtaatgaaa tcgaagccgt acaggatatg
    14161 ttgcaacgtg cacagatgag cgacgaagag cgcaacgccg cctccgagct tgcccgccgt
    14221 ttggttaccc aagtccgcgc cggccgcacc aaagccggcg gcgtggatgc gctgatgcac
    14281 gagttttcac tctccagcga agaaggcatc gcgctgatgt gtctggcaga agccctgctg
    14341 cgtatccccg acaacgccac gcgcgaccgc ctgattgccg acaagatttc agacggcaac
    14401 tggaaaagcc atttgaacaa cagcccttcc ctcttcgtca acgccgccgc ctggggcctg
    14461 ctgattaccg gcaaactgac cgccacaaac gacagacaaa tgagttccgc actcagccgc
    14521 ctgatcagca aaggcggcgc accgctcatc cgccaaggcg taaattacgc catgcgcctg
    14581 ttgggcaaac agttcgtaac cggacagacc attgaagaag ccctgcaaaa cggcaaagaa
    14641 cgcgaaaaaa tgggctaccg cttctccttc gatatgttgg gcgaagccgc ctacacccaa
    14701 gccgatgccg accgctacta ccgcgactat gtcgaagcca tccacgccat cggcaaagat
    14761 gcggcaggac aaggcgttta cgaaggcaac ggcatttccg tcaaactctc cgccatacat
    14821 ccgcgctact cgcgcgccca acacggccgt gtgatgggcg aactgttgcc gcgcctgaaa
    14881 gagctgttcc ttttgggtaa aaaatacgat atcggtatca acatcgatgc cgaagaagcc
    14941 aaccgtctgg agctgtcttt ggatttgatg gaggcactcg tttccgatcc cgatttggca
    15001 ggctacaaag gcatcggttt cgttgttcaa gcctaccaaa aacgttgtcc gttcgttatc
    15061 gactacctga tcgaccttgc ccgccgcaac aaccaaaaac taatgatccg cctcgtcaaa
    15121 ggcgcgtatt gggacagcga aatcaaatgg gcgcaagtgg acggcttgaa cggctatccg
    15181 acctacaccc gcaaagtcca caccgacatc tcctacctcg cctgcgcgcg caaactgctt
    15241 tccgcgcaag acgcggtatt tccgcaattc gccacccaca acgcctacac cttgggcgca
    15301 atctaccaaa tgggcaaagg caaagacttt gaacaccaat gtctgcacgg catgggcgaa
    15361 accctgtacg accaagtcgt cggcccgcaa aacttaggcc gccgcgtgcg cgtgtacgcc
    15421 ccggtcggca cacacgaaac cctgctcgcc tacttggtgc gccgcctgtt ggaaaacggc
    15481 gcgaactcgt ctttcgtcaa ccaaatcgtc gatgaaaaca tcagcatcga cacgctcatc
    15541 cgcagcccgt tcgacaccat cgccgaacaa ggcatccacc tgcacaacgc cctgccgctg
    15601 ccgcgcgatt tgtacggcaa atgccgtctg aactcgcaag gcgtggactt gagcaacgaa
    15661 aacgtattgc agcagcttca agaacagatg aacaaagccg ccgcgcaaga cttccacgcc
    15721 gcatccatcg tcaacggcaa agcccgcgat gtcggcgaag cgcaaccgat taaaaaccct
    15781 gccgaccacg acgacatcgt cggcacagtc agctttgccg atgccgcgct tgcccaagaa
    15841 gcggttggcg cagccgttgc cgcgttcccc gaatggagtg cgacacctgc cgccgaacgc
    15901 gccgcctgcc tgcgccgttt cgccgacctg ctcgaacagc acacccccgc gctgatgatg
    15961 cttgccgtgc gcgaagcagg caaaacgctg aacaacgcca ttgccgaagt gcgcgaagcc
    16021 gtcgatttct gccgctacta cgcaaacgaa gccgaacata ccctgcctca agacgcaaaa
    16081 gccgtcggcg cgattgtcgc catcagcccg tggaacttcc cgctcgccat ctttaccggc
    16141 gaagtcgttt ccgcattggc ggcaggcaac accgtcatcg ccaaacccgc cgaacaaacc
    16201 agcctgattg ccggttatgc cgtttccctc atgcacgaag ccggcatccc gacttccgcc
    16261 ctgcaactcg tcctcggcgc aggcgacgtg ggtgcggcat tgaccaacga tgcccgcatc
    16321 ggcggcgtga ttttcaccgg ctcgaccgaa gtggcgcgcc tgatcaacaa agcccttgcc
    16381 aaacgcggcg acaatcccgt cctgattgcc gaaaccggcg gacaaaacgc catgattgtc
    16441 gattccaccg cacttgccga acaagtctgc gccgacgtat tgaactccgc cttcgacagc
    16501 gcgggacaac gctgctccgc cctgcgtatc ttgtgcgtcc aagaagacgt tgccgaccgt
    16561 atgctcgaca tgatcaaagg cgcgatggac gaactcgtcg tcggcaaacc gattcagctc
    16621 actaccgatg tcggccccgt catcgatgcc gaagcacagc aaaacctgtt gaaccacatc
    16681 aacaaaatga aaggcgttgc caagtcttac cacgaagtca aaaccgctgc cgatgtcgat
    16741 tccgaaaaat ccacgttcgt ccgccccatc ctgtttgaat tgaacaacct caacgaactg
    16801 caacgcgaag tcttcggtcc cgtcctgcac gtcgtccgct accgcgccga caaactcgac
    16861 agcgtcatcg accaaatcaa cagcaaaggc tacgccctga cccacggcgt acacagccgc
    16921 atcgaaggca cagtacgcca catccgcagc cgcatcgaag ccggcaacgt ttacgtcaac
    16981 cgcaacatcg tcggcgcagt cgtcggcgta cagcccttcg gcggacacgg tctgtccggc
    17041 acaggcccca aagcaggcgg ttcgttctac ctgcaaaaac tgacccgcat ccccgaatgg
    17101 gttgccccga ccctgagcca aatcggacag gcagacgaag ccgcgctcaa acgcctcgaa
    17161 gcactggttc acaaactgcc gttcaacgcc gaagagaaaa aagccgcagc ggccgctttg
    17221 ggacacgccc gcatccgcac cctgcgccgt gccgaaaccg tccttaccgg cccgaccggc
    17281 gagcgcaaca gcatctcatg gcacgcgcca aaacgcgtct gggtacacgg cggcagcctc
    17341 ctgcaagcct tctgcgccct aaccgaactt gccgcctccg gcgtacaggc agtggtcgaa
    17401 cccgacagcc cgctggcttc ctacaccgcc gacttggaag gtctgctgct ggtcaacggc
    17461 aaacccgaaa ccgccgacat cagccacgtt gccgccctgt cgcctttgga cagcgcgcgc
    17521 aaacaggaac ttgccgccca cgacggcgca ctcatccgca tcctcccttc ggaaaacgga
    17581 ctcgacatcc tgcaagtgtt tgaagaaatc tcttgcagcg tcaacaccac agccgccggc
    17641 ggcaacgcca gcctgatggc ggttgccgac tgattttacc gaaatccccg agcgcggccc
    17701 gtgaaccaat gccgtctgaa aacctttcag acggc
//
LOCUS       DAWHXZ010000017        17310 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000017 DAWHXZ010000000
VERSION     DAWHXZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.017"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            49..124
                     /locus_tag="WIE35_000379"
     tRNA            49..124
                     /locus_tag="WIE35_000379"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82..84,aa:Trp,seq:cca)
     gene            239..517
                     /gene="secE"
                     /locus_tag="WIE35_000380"
     CDS             239..517
                     /gene="secE"
                     /locus_tag="WIE35_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ4153348.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            519..1055
                     /gene="nusG"
                     /locus_tag="WIE35_000381"
     CDS             519..1055
                     /gene="nusG"
                     /locus_tag="WIE35_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ4153349.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1156..1590
                     /gene="rplK"
                     /locus_tag="WIE35_000382"
     CDS             1156..1590
                     /gene="rplK"
                     /locus_tag="WIE35_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ4153350.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1590..2285
                     /gene="rplA"
                     /locus_tag="WIE35_000383"
     CDS             1590..2285
                     /gene="rplA"
                     /locus_tag="WIE35_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ4153351.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKTTRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2515..3015
                     /gene="rplJ"
                     /locus_tag="WIE35_000384"
     CDS             2515..3015
                     /gene="rplJ"
                     /locus_tag="WIE35_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ4153352.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3073..3444
                     /gene="rplL"
                     /locus_tag="WIE35_000385"
     CDS             3073..3444
                     /gene="rplL"
                     /locus_tag="WIE35_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ4153353.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3635..7813
                     /gene="rpoB"
                     /locus_tag="WIE35_000386"
     CDS             3635..7813
                     /gene="rpoB"
                     /locus_tag="WIE35_000386"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ4153354.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVADRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDKDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNKLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            7970..12145
                     /gene="rpoC"
                     /locus_tag="WIE35_000387"
     CDS             7970..12145
                     /gene="rpoC"
                     /locus_tag="WIE35_000387"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ4153355.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVNQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGAAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12437..12694
                     /locus_tag="WIE35_000388"
     CDS             12437..12694
                     /locus_tag="WIE35_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153356.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGNRMGMPINIG"
     gene            12691..12840
                     /locus_tag="WIE35_000389"
     CDS             12691..12840
                     /locus_tag="WIE35_000389"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ4153357.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            13019..13390
                     /gene="rpsL"
                     /locus_tag="WIE35_000390"
     CDS             13019..13390
                     /gene="rpsL"
                     /locus_tag="WIE35_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ4153358.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13508..13978
                     /gene="rpsG"
                     /locus_tag="WIE35_000391"
     CDS             13508..13978
                     /gene="rpsG"
                     /locus_tag="WIE35_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ4153359.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            13997..16102
                     /gene="fusA"
                     /locus_tag="WIE35_000392"
     CDS             13997..16102
                     /gene="fusA"
                     /locus_tag="WIE35_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ4153360.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            16190..>17310
                     /gene="tuf"
                     /locus_tag="WIE35_000393"
     CDS             16190..>17310
                     /gene="tuf"
                     /locus_tag="WIE35_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ4153361.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLR"
BASE COUNT         4603 a         3901 c         4478 g         4328 t
ORIGIN      
        1 cgtaccgtgg gtgccggcgt ggtttcttct gttatcgctt aagtttagag gccaatagct
       61 caattggtag agtatcggtc tccaaaaccg agggttgggg gttcgagacc ctcttggcct
      121 gccaaataaa aaattaaccg gccttgtgtc ggttaatttt tttgtatttg ttatttagta
      181 aactctcttg ccatttacat ggattgagaa tagacagatg ctatgatgga taaataatat
      241 gacagaacat acgcctgaaa aaaagaacgt taaagtggat caactggttg ttcaagataa
      301 agaatctgca tctaattccg gtaaggaagg gttttttgca tatttctcaa attcttggtc
      361 cgaattcaaa aaggtggttt ggcctaagcg tgaagatgct gtcagaatga ctgtatttgt
      421 tatagtgttt gttgctgtgc tttctatatt tatctatgcg gcagatacag caatttcgtg
      481 gttatttttt gatgtattgc tgagaaggga aggttgagat gtcgaaaaaa tggtatgttg
      541 tacaggcgta ttcggggttt gagaagaatg tccaacgaat attggaagag cgcattgccc
      601 gtgaggagat gggagattat ttcggacaaa ttctggtgcc tgtagagaaa gttgttgata
      661 tccgcaatgg tcgtaagact attagtgaaa gaaagtcata tcctggttat gtgctagttg
      721 agatggaaat gacagatgac tcttggcatc ttgtaaaaag caccccccgt gtttccggtt
      781 ttattggagg gagggctaat agacctacgc cgattagtca gagagaggct gaaattattt
      841 tacagcaggt tcagaccggc atagagaagc cgaaaccaaa agttgaattt gaggtcggtc
      901 aacaggttcg tgtaaatgaa gggccgtttg cggattttaa cggggtggtt gaggaggtca
      961 attatgaacg gaataagtta cgcgtgtctg ttcagatatt tggtagagaa acacccgttg
     1021 agctggagtt cagccaggtt gaaaagatta actgattttt atacttgaaa aaaaagcaat
     1081 aagaggatag aatcaaaaat taacttgggg agcggaaatg gttccgcgtc ttacccgttt
     1141 ttaggagttc gttaagtggc aaagaaaatt atcggctata ttaaactgca aattcctgca
     1201 ggtaaagcca atccatctcc tccggttggt cctgctttgg gtcagcgcgg tttgaatatt
     1261 atggaatttt gtaaggcatt taatgctgca acccaaggta tggagcctgg cttaccgatt
     1321 ccggttgtga ttactgcatt tgcagataaa tcattcacat ttgtgatgaa aaccccgcca
     1381 gcttctatct tgttgaaaaa ggctgccggt ttgcaaaaag gtagttctaa tcctctgacc
     1441 aacaaagtgg gtaaattgac ccgtgcccag ttggaagaaa ttgctaaaac taaagatcct
     1501 gatttgactg ctgctgactt ggatgcggct gtccgtacta tagcaggttc tgctcgctca
     1561 atgggcttgg atgtggaggg tgttgtataa tggctaaagt atctaaacgc ttgaaagctc
     1621 ttcgctcttc tgtggaagcc aataaattat atgcaattga tgaagcaatt gctttggtaa
     1681 aaaaagcagc gactgctaaa tttgacgagt ctgttgacgt atctttcaac ttgggcgttg
     1741 atccgcgtaa atctgaccaa gttatccgtg gttcggtcgt tctgcctaaa ggcaccggta
     1801 agacaacccg tgtggctgta tttactcaag gtgcaaatgc agaagctgct aaagaagctg
     1861 gtgcagatat cgtcggtttc gaagatttgg ctgctgaaat caaagcaggc aatctgaact
     1921 ttgacgttgt tattgcttct cccgatgcaa tgcgtattgt tggtcaattg ggtaccatct
     1981 tgggccctcg tggtctgatg ccaaacccta aagtaggtac ggttacccct aatgttgccg
     2041 aagcagtaaa aaatgcaaaa gcaggtcaag tgcagtaccg taccgacaag gcaggtattg
     2101 ttcatgcaac gattggtcgt gcttcattcg ctgaagctga tttgaaagag aactttgatg
     2161 cgttgctgga tgctatcgtt aaagccaagc ctgctgccgc taaaggtcag tatctgaaaa
     2221 aagttgctgt gtctagcacc atgggtttgg gtattcgcgt tgatacatca agcgtaaata
     2281 actaatctta aggaattttc aagcagtttg gttttctggg ctgcttgaat ttgggctact
     2341 taaaattaag tagatgtcca agaccgtagg gatcgtaaga tttaatcgta actgccctac
     2401 gcagacggta gtcctgaaac acattgcaag attgcttgta agatgtcttt ttaggttacc
     2461 gcgctggtgg gatatcgttt tggtatcctg tttataaaca gtgggaggta gaccttgagt
     2521 ctcaatattg aaaccaagaa agtggcggtc gaggaaatta gcgcggcaat tgctaatgct
     2581 caaaccctcg tagtcgctga atatcgcggt atcagtgttt ccagtatgac tgagcttcgt
     2641 gcgaatgcac gtaaagaagg cgtttatttg cgcgttctga aaaatacttt ggctcgtcgt
     2701 gcagtgcaag gtacttcatt tgcagaattg gccgatcaaa tggttggtcc gttggtttac
     2761 gctgcttctg aagatgctgt tgctgctgct aaagtgttgc accaattcgc gaaaaaagat
     2821 gacaaaattg tcgttaaagc cggttcttac aatggcgaag taatgaatgc tgctcaggtt
     2881 gctgagttgg cttctattcc gagccgcgaa gagctgttgt ccaaactgtt gttcgttatg
     2941 caagctcctg tatcgggctt tgcgcgcggt ttggctgctt tggcagagaa aaaagccggc
     3001 gaagaagccg cttaatcgat tttgtttctg ttaatcaatt attttttaat acaatatttg
     3061 gagtaaaata gcatggctat tactaaagaa gacattttgg aagcagttgg ttctttgacc
     3121 gtaatggaat tgaacgactt ggttaaagct tttgaagaaa aattcggtgt ttctgctgct
     3181 gctgttgcag ttgcaggtcc tgctggtgcc ggtgctgccg atgctgaaga aaaaaccgaa
     3241 tttgatgtcg ttttggcttc tgccggcgat caaaaagtcg gcgtgattaa agttgtccgt
     3301 gcaattaccg gtttgggtct gaaagaagct aaagacatcg ttgacggtgc acctaaaacc
     3361 attaaagagg gtgtttctaa agctgaagcc gaagacatcc aaaaacaact ggaagaagcc
     3421 ggcgctaaag tcgaaatcaa ataatttgat gcttcttatg aaggctggca gttttctgcc
     3481 agccttattt tgcttcttaa aataagcatt aagtattgtt tacatttatt tgcatagttt
     3541 ttatcaaatc attgcaaata aatgtaaaca tcagattgat gcgtgccgtt gctttcagac
     3601 ggcctattat tgaaaattac ttttcggagt gtatatgagc tattcgttta ccgagaaaaa
     3661 acgtatccgt aagagttttg caaagcggga aaatgttttg gaagttcctt tcctgctggc
     3721 aacccaaatt gattcttatg cgaagttttt gcagctggaa aatgcttttg acaaacgtac
     3781 cgatgacggt ctgcaggcgg catttaattc tattttcccg attgtgagcc ataacggtta
     3841 tgcgcgattg gagtttgtgc attacacatt gggcgagcct ttgttcgata ttcccgaatg
     3901 tcagttgcgc ggaatcactt atgcagcccc cttgcgcgcg cgtatccgtt tggtgatttt
     3961 ggataaggaa gcatctaaac cgacggtaaa agaagttcgt gaaaacgaag tgtatatggg
     4021 cgaaattccg ttgatgaccc cgagcggttc ttttgtgatt aacggcacag agcgtgtgat
     4081 tgtctcccag ttgcaccgtt cgcccggtgt attcttcgag catgacaaag gtaagacgca
     4141 ctcttccggc aaattgttat tctccgcccg catcattccc taccgtggtt catggttgga
     4201 ttttgaattt gatccgaaag atttgctgta tttccgtatc gaccgccgcc gtaaaatgcc
     4261 ggtaacgatt ttgttgaagg ctttgggcta caacaatgag caaatcttgg atattttcta
     4321 cgacaaagaa acgttctatt tgtcttcaaa cggtgttcaa accgatttgg tcgcagaccg
     4381 tctgaaaggc gaaactgcca aggtcgatat cttggataaa gaaggcaatg tattggttgc
     4441 caaaggtaag cgcattactg cgaaaaatat ccgtgatatt accaatgcag gcctgacccg
     4501 tttggatgta gaaccggaaa gcctgctggg caaagcattg gctgccgatc tgattgattc
     4561 ggaaaccggc gaggtattgg cttctgccaa tgatgaaatt acagaagagt tgttggccaa
     4621 atttgatatc aacggcgtaa aagaaattac gaccctttat atcaatgagc tggatcaggg
     4681 tgcttatatc tccaatacct tgcgtacgga tgagactgcc ggccggcagg cggctcgtgt
     4741 tgcgatttac cgtatgatgc gtccgggcga accgcccacc gaagaggcgg tcgagcaatt
     4801 gtttaaccgc ttgttcttca gtgaagacag ctacgatctg tcccgcgtag gccgtatgaa
     4861 atttaatacg cgcacatacg aacaaaaact gtccgaagcc caacaaaact cttggtacgg
     4921 ccgcctgctg aacgaaacgt ttgccggtgc tgccgacaaa ggcggttatg tcctgagcgt
     4981 cgaagatatt gtcgcctcga ttgcgacttt ggtcgagttg cgtaacggcc atggcgaagt
     5041 ggacgatatc gatcacttgg gcaaccgccg agtacgttcg gtaggcgagc tgactgaaaa
     5101 ccaattccgt agcggtttgg cccgtgtgga acgtgccgta aaagaacgtt tgaatcaggc
     5161 ggaatcagaa aacttgatgc cgcacgattt gattaatgca aaacctgttt ctgccgctat
     5221 taaagaattc ttcggctcca gccaattgag tcagtttatg gatcagacca accccttgtc
     5281 tgaagtaacc cataaacgcc gtgtatctgc attgggtccg ggcggtttga cccgcgaacg
     5341 tgcaggattt gaggtgcggg acgtgcatcc gacccactac ggtcgcgtat gtccgattga
     5401 aacgcctgaa ggtccgaaca tcggtttgat caactcattg tccgtttatg cgcgcaccaa
     5461 tgattacggt ttcttggaaa cgccttaccg ccgcgttatc gacggcaaag taactgaaga
     5521 aatcgactac ttgtctgcca tcgaagaagg ccgctatgtg attgcacagg cgaatgccga
     5581 tttggacaaa gacggcaatc tgattggcga cttggttact tgtcgtgaaa aaggcgaaac
     5641 cattatggca acgcctgacc gcgttcaata tatggacgtg gcaaccggtc aagtggtatc
     5701 cgttgcagca tccctgattc cgttcttgga acacgatgac gcgaaccgcg cattgatggg
     5761 tgcgaatatg caacgtcagg cagtgccttg cttgcgtcct gaaaaaccga tggtcggtac
     5821 cggtatcgag cgttccgttg ccgttgactc tgctactgca atcgttgccc gccgaggcgg
     5881 cgtggtcgag tatgtcgatg ccaaccgtgt tgtgattcgt gtccatgacg acgaagcgac
     5941 tgccggcgaa gtgggtgtcg atatttacaa cttggttaaa tttacccgtt ccaaccagtc
     6001 taccaatatc aatcagcgtc ctgccgtcaa agccggcgat gttttgcaac gcggcgattt
     6061 ggtggccgat ggcgcgtcca ccgatttggg cgaattggct ttgggtcaaa atatgaccat
     6121 cgccttcatg ccgtggaacg gttacaacta cgaagactcg attctgattt ccgaaaaagt
     6181 ggctgcggac gaccgctata cttcgattca cattgaggaa ttgaatgtcg ttgcccgcga
     6241 taccaagctg ggtgcggaag acattacccg cgatattccg aacttgtccg agcgtatgca
     6301 aaaccgtttg gacgaatccg gtatcgttta catcggtgcg gaagtagaag ccggcgatgt
     6361 gttggtaggc aaggtaacgc ctaaaggcga aacccagctg acgccggaag aaaaactgct
     6421 gcgcgccatc ttcggtgaaa aagcatctga cgtaaaagat acttcattgc gtatgcctac
     6481 cggcatgagc ggtaccgtta tcgacgttca agtcttcact cgtgaaggta ttcaacgcga
     6541 caaacgtgct caatccatta tcgattccga gttgaaacgc taccgtttgg atttgaacga
     6601 ccaattgcgt attttcgaca acgacgcatt cgaccgtatc gagcgtatga ttgtcggtca
     6661 gaaagccaac ggcggcccga tgaagctggc caaaggcagt gaaatcacga ccgaatatct
     6721 ggcgggtctg ccgagcaggc acgattggtt cgatatccgt ctgaccgatg aagatttggc
     6781 caagcagttg gaactgatta aagtgagcct gcaacaaaaa cgcgaagaag cggacgagtt
     6841 atacgaaatc aagaagaaaa aactgaccca aggcgacgaa ttgcaacccg gcgtacaaaa
     6901 aatggtgaaa gtttttatcg ccatcaaacg ccgtctgcaa gccggcgaca aaatggcggg
     6961 ccgccacggt aacaaaggcg tggtatcgcg cattctgcca gtggaagaca tgccttacat
     7021 ggcggacggc cgtccggtag acatcgtact gaacccattg ggcgtacctt cccgtatgaa
     7081 catcggtcag attttggaag ttcacttggg ttgggcagca aaaggtatcg gcgagcgtat
     7141 cgaccgtatg ctgaaagagc aacgcaaagc aggcgagttg cgcgagttct tgaacaaact
     7201 ctacaacggc agcggcaaga aagaagattt ggatgccctg actgatgaag aaatcatcga
     7261 actggcctcc aacctgcgca aaggtgcatc tttcgcctct cctgtattcg acggtgcgaa
     7321 agagtctgaa atccgcgaaa tgttgaactt ggcttatccg agcgacgatc ctgaggttga
     7381 aaaactgggc ttcaacgaca gtaaaaccca aatcacgctg tatgacggcc gttcaggcga
     7441 agcatttgac cgcaaggtta cagtaggtgt gatgcactat ctgaaactgc accacttggt
     7501 tgacgaaaaa atgcacgcgc gttctaccgg tccgtacagt ctggttaccc agcagccttt
     7561 gggcggtaaa gcccagttcg gcggccaacg tttcggtgag atggaggttt gggcactgga
     7621 agcatacggc gcagcctaca cgctgcaaga gatgctgact gtgaagtctg acgacgtgaa
     7681 cggccgtacc aaaatgtacg aaaacatcgt caaaggcgaa cacaaaatcg atgccggtat
     7741 gcccgagtcc ttcaacgtat tggtcaaaga gattcgctca ctgggcttgg atatcgattt
     7801 ggaacgttac taaacaaaag ttttcagacg gcctttcagg gtcgtctgaa aaagtggttt
     7861 cagaataaga atgaagcaat cggcatttag gccgtctgaa atcaaaagta ccgtttcccc
     7921 atatcgaaaa tccgccatgc ggtaaaaata cttccttcaa ggagcaaaaa tgaatttgtt
     7981 gaacttattt aatccgttgc aaactgccgg catggaagaa gagtttgatg ccattaaaat
     8041 cggtattgcc tctcccgaaa ccatccgctc atggtcttat ggcgaagtta aaaaacctga
     8101 aaccatcaac taccgtacgt tcaaacctga gcgtgacggt ttgttctgtg ccaaaatctt
     8161 tggcccggtc aaagactacg aatgcttgtg cggaaaatac aaacgcttga aatttaaagg
     8221 tgtaacttgt gaaaaatgcg gcgtggaagt aaccctgtcc aaagtgcgcc gcgaacgcat
     8281 gggccatatc gaattggctg cgcccgtcgc acatatttgg ttcttaaaat ccctgccttc
     8341 ccgcttgggt atggtgttag acatgacttt gcgcgatatc gaacgcgtat tgtactttga
     8401 agcatttgtg gtaaccgatc ccggtatgac tccgctgcaa cgccgccaat tgctgactga
     8461 agacgattac tacaacaagc tggacgaata cggcgacgac ttcgatgcca aaatgggtgc
     8521 agaaggtatc cgcgaattgc tgcgtacctt gaatgtagca ggcgaaatcg aaatcctgcg
     8581 ccaagagttg gaatcgaccg gttccgacac caaaatcaaa aaaatcgcca aacgcttgaa
     8641 agtattggaa gctttccatc gttccggtat gaagctggaa tggatgatta tggatgtgct
     8701 gccggtattg ccgcctgatt tgcgtccgtt ggttccattg gatggcggtc gttttgccac
     8761 ttccgatttg aacgatttgt accgtcgcgt catcaaccgt aacaaccgtc tgaaacgtct
     8821 gttggaactg catgcgcccg acattatcgt ccgcaacgaa aaacgtatgt tgcaagaagc
     8881 agttgactcg ctgttggata acggccgtcg cggtaaagcc atgaccggcg ccaacaaacg
     8941 cccgctgaaa tcattggcag acatgattaa aggtaaaggc ggtcgcttcc gtcaaaacct
     9001 gttgggcaaa cgtgtggact actctggccg ttccgtgatt accgtaggcc catacctgcg
     9061 tctgcaccaa tgcggtctgc cgaaaaaaat ggctttggaa ttgttcaaac cgttcatttt
     9121 ccacaaattg gaaaaacaag gtttggcttc tactgttaaa gctgcgaaaa aattggtaga
     9181 acaagaagta cctgaagtat gggatatctt ggaagaagtc atccgcgaac atccgattat
     9241 gttgaaccgt gcgccaaccc tgcaccgttt gggtattcaa gcgttcgaac ccatcctgat
     9301 tgaaggtaaa gccattcagt tgcatccgtt ggtatgtgcc gcatttaacg ccgactttga
     9361 cggcgaccaa atggcggtac acgttccatt gagcttggaa gcacaaatgg aagcacgcac
     9421 gctgatgctg gcttcaaaca acgtattgtc tcctgccaac ggcgaaccga ttatcgtacc
     9481 ttcccaagac atcgtattgg gtctgtacta catgacccgc gaccgtatca atgccaaagg
     9541 cgagggcagc ctgtttgccg atgtgaaaga agtgcatcgc gcataccata ccaaacaggt
     9601 cgagctgggt acgaaaatta ccgtacgtct gcgcgaatgg gtgaaaaacg aagcaggtga
     9661 gtttgagcct gtcgttaacc gttacgaaac aaccgtcggc cgtgcattgt tgagcgaaat
     9721 cctgccgaaa ggcctgccgt ttgaatatgt caacaaagcg ttgaagaaaa aagaaatttc
     9781 taaactgatt aacgcatcgt tccgcctgtg cggcttgcgc gatacggtta tctttgctga
     9841 ccacctgatg tacaccggtt tcggatttgc ggcaaaaggc ggtatttcca ttgccgttga
     9901 cgatatggaa attccaaaag aaaaagcggc cttgctggct gaagccaatg ccgaggttaa
     9961 agaaatcgaa gaccaatacc gtcaaggttt ggttaccaac ggcgaacgct acaacaaggt
    10021 ggtcgatatt tggggtcgtg ccggcgataa aatcgctaaa gcgatgatgg acaacctgtc
    10081 caaacagaaa gttatcgacc gtgacggcaa cgaagtcaat caagagtcat tcaactccat
    10141 ctatatgatg gcggactccg gtgcccgtgg ttctgcggct cagattaaac agttgtccgg
    10201 tatgcgcggg ttgatggcta aacctgacgg ctcgattatt gaaacgccga ttacctcaaa
    10261 cttccgcgaa ggtttgaccg tattgcaata ctttattgcg actcacggtg cgcgtaaagg
    10321 tttggcggat accgcgttaa aaaccgcgaa ctccggttac ctgacccgtc gtctggtaga
    10381 cgtaactcaa gatttggtcg ttgttgaaga cgattgcggt acttcagacg gctttgtcat
    10441 gaaggcagtg gtacaaggcg gtgatgtgat tgaagcattg cgcgatcgta ttttgggccg
    10501 tgttactgcg tctgacgttg tcgatccgtc aagcggcgag accttggttg aagccggtac
    10561 gttgttgact gaaaaactgg tggatatgat tgaccaatcc ggtgtcgatg aagtcaaagt
    10621 ccgtactccg attacttgta aaacccgcca cggtttgtgc gcgcactgtt atggtcgcga
    10681 cttggcgcgc ggtaagctgg ttaacgccgg tgaagcagtt ggtgtgattg ctgcacaatc
    10741 cattggtgaa ccgggtaccc agttgaccat gcgtacgttc cacattggtg gtgcggcatc
    10801 ccgtgcggca gcagccagcc aagtggaagc caaatccaac ggtacggcac gtttcagcag
    10861 ccagatgcgt tacgttgcca acaacaaagg tgagttggtt gtcatcggtc gctcttgcga
    10921 agtcgtgatt catgatgaca tcggccgtga acgcgaacgc cacaaagtac cttacggtgc
    10981 catcctgctg gtacaagacg gagcggccat taaagccggt caaaccttgg caacctggga
    11041 tccgcatacc cgtccgatga tcaccgaaca tgcaggtatg gtgaaatttg agaacgtgga
    11101 agagggtgtt accgttgcga aacaaactga cgatgtaacc ggtttgtcca ctctggtagt
    11161 gattgacggt aaacgtcgtt cctctagtgc ttctaaactg ctgcgtccga ctgtaaaatt
    11221 gttggatgaa aacggcgtgg aaatctgtat tcccggtacg tccactccgg tttcgatggc
    11281 attccccgtt ggtgcggtga ttaccgtacg cgaaggtcag gaaatcggta aaggcgacgt
    11341 attggcgcgt atcccgcaag cctcttccaa aacccgcgac attaccggtg gtctgccgcg
    11401 cgttgccgaa ttgtttgaag cacgcgtgcc gaaagatgcc ggtatgttgg cggaaattac
    11461 cggtaccgtt tccttcggca aagagaccaa aggcaagcaa cgtctgatta ttaccgacgt
    11521 ggacggcgta gcatacgaga ctttgatttc caaagagaaa cagattttgg tacacgacgg
    11581 tcaagtggta aaccgcggcg aaaccattgt ggacggcgcg gtcgatccgc acgatattct
    11641 gcgtctgcaa ggtatcgaag cactggcacg ctacattgtc caagaggtgc aagaggttta
    11701 ccgtctgcaa ggtgtgaaga tttctgataa acacatcgaa gtcatcatcc gtcaaatgtt
    11761 gcgccgtgtg aacattgcgg atgccggcga aaccggcttc attaccggag agcaggtcga
    11821 acgcggcgat gtgatggcgg ccaatgaaaa agctttggaa gaaggcaaag aaccggcgcg
    11881 ttacgaaaac gtattgctgg gtattaccaa agcttccctg tctaccgaca gcttcatttc
    11941 tgctgcatcg ttccaagaaa cgacccgtgt tctgaccgaa gccgcgatta tgggcaaaca
    12001 agacgaactg cgcggtttaa aagagaacgt catcgtcggc cgcctgattc ctgccggtac
    12061 cggtttgact tatcaccgca gccgtcatca gcaatggcaa ggggtagagc aggagactgc
    12121 tgaaacccaa gtaacggatg aataatcttc ggcgcgttta ttcaatacaa aaccgcaagc
    12181 tttaagcttg cggtttttct ttgtccgatt aaggcaaaaa caagcgtttt cgtcattttg
    12241 aggcgtgtgg attattcctt aggtattttc gggccggaga ccaacgaggt ggcgggtgtc
    12301 gtcggtacgt ccggagacca aaataacttt gccagggatg ttggtttcgg cggtcaaaaa
    12361 aagtagcgtc ttaatgtttt ccatttaaac aaatgtcgtc tgaaacttca gacggcattt
    12421 cctttaaaaa ataaatatga aacccagaaa tctctttttt gcaggctgcc tgctgacttc
    12481 ggcgacgttt gccgaggata tcggcgtacc tgtcgaactg attaacgtcg gtaatcggat
    12541 tgcgatgccg tctgaagggg aaagcctcgc cctcctgccg tttgccgagg atgtaccgcc
    12601 ggttcgcgat gcaatgccgt ctgaagttcc taaaagcgcg gcaggcggcg atgttcgggg
    12661 taaccggatg ggaatgccga ttaacatcgg atgagcgcgg ctttatggca taaaaaactg
    12721 tcgtggaaag gatttacacc ccaaataaat ttccgttaca acaagatcaa cagcaatatg
    12781 cccgcctttt attcgcgcag cggcaaggaa cggtttgtca gtatagaaaa aacgtattga
    12841 cagtattttc ttcagtcgtc cgactgattg tgagggatgt tggtaaatat ttatcggcaa
    12901 acaagaaaat catctttctt cttgtcgtta tgcttgactg tatacttgca ataaaaatat
    12961 aattccactc ttgccgacat ggtgtcggca agtatttaac tcaacaggac gagaaaatat
    13021 gccaactatc aaccaattgg tacgcaaagg ccgtcaaaag cccgtgtacg taaacaaagt
    13081 gcccgcactg gaagcctgcc cgcaaaaacg cggcgtgtgc acccgtgtat acacgactac
    13141 ccctaaaaaa cctaactctg cattgcgtaa agtatgtaaa gtccgcctga ccaacggttt
    13201 tgaagtcatt tcatacatcg gcggcgaagg ccacaacctg caagagcaca gtgtcgtatt
    13261 gattcgcggc ggtcgtgtaa aagacttgcc aggtgtgcgt taccacactg tacgcggttc
    13321 tttggatact gcaggtgtta aagaccgtaa acaagcccgt tccaaatatg gtgctaagcg
    13381 tcctaaataa ttactgggac ttaaataggc acgtcggccg cctaagcaga acaacggccg
    13441 agtaagtgaa tactcaattg ggtattcatg ggaatagacc cgactgaata gattaaagga
    13501 aattaaaatg ccaagacgta gagaagtccc caagcgcgac gtactgccag atcctaaatt
    13561 cggcagcgtc gagttgacca aattcatgaa cgtattgatg attgacggta aaaaatccgt
    13621 tgccgagcgt atcgtttacg gtgcgttgga acagattgag aaaaaaaccg gcaaagtagc
    13681 aatcgaagta tttaacgaag ccattgcaaa cgccaaacct atcgtggaag tgaaaagccg
    13741 ccgtgtaggt ggtgcaaact accaagttcc tgttgaagtt cgtccttcac gccgtttggc
    13801 tttggcaatg cgctgggttc gcgatgcggc ccgcaaacgt ggtgagaaat ccatggacct
    13861 gcgtttggca ggcgaattga ttgatgcgtc cgaaggccgt ggcggtgcgt tgaaaaaacg
    13921 tgaagaagta caccgtatgg ctgaagccaa caaagcattc tctcacttcc gtttctaatt
    13981 ttgaaaggct aataaaatgg ctcgtaagac cccgatcagc ctgtaccgta acatcggtat
    14041 ttccgcccat atcgatgcgg gtaaaaccac gacgacagaa cgtattttgt tctataccgg
    14101 tttgacccac aagctgggcg aagtgcatga cggtgcggct actaccgact acatggaaca
    14161 agagcaagag cgcggtatta ccattacctc cgctgccgtt acttcctact ggtccggtat
    14221 ggcgaaacaa ttccccgagc accgcttcaa catcatcgac accccgggac acgttgactt
    14281 taccgtagag gtagagcgtt ctatgcgtgt attggacggc gcggtaatgg tttactgcgc
    14341 ggtgggcggt gttcaacccc aatctgaaac cgtatggcgg caagccaaca aataccaagt
    14401 gccgcgcttg gcgtttgtca ataaaatgga ccgtcagggt gccaacttct tccgcgttgt
    14461 cgagcaaatg aaaacccgtt tgcgcgcaaa ccctgtacct atcgtcattc cggttggtgc
    14521 ggaagacaac ttcagcggtg tggttgattt gttgaaaatg aaatccatca tttggaatga
    14581 agccgataaa ggtacaacct ttacctatgg cgatattcct gccgaattgg tcgaaactgc
    14641 cgaagaatgg cgtcaaaata tgattgaagc cgcagccgaa gccagcgaag aactgatgga
    14701 caaatactta ggcggcgacg agctgaccga agaagaaatc gtaggcgcgt tgcgtcaacg
    14761 tactttggca ggcgaaattc agcctatgct gtgtggttct gcatttaaaa acaaaggtgt
    14821 tcaacgtatg ttggacgcag ttgtagaatt gctgccagct cctaccgata ttcctccggt
    14881 tcaaggtgtc aacccgaata ccgaggaagc cgacagccgt caagccagcg atgaagagaa
    14941 attctctgca ttggcgttca aaatgttgaa cgacaaatac gtcggtcagc tgacctttat
    15001 ccgcgtttac tcaggcgtag taaaatccgg cgataccgta ttgaactccg taaaaggcac
    15061 tcgcgaacgt atcggtcgtt tggtacaaat gactgccgca gaccgtactg aaatcgaaga
    15121 agtacgcgcc ggcgacatcg cagccgctat tggtctgaaa gacgttacta ccggtgaaac
    15181 cttgtgtgcg gaaagcgcgc cgattatctt ggaacgtatg gaattccccg agccggtaat
    15241 ccatattgcc gttgagccga aaaccaaagc cgaccaagag aaaatgggta tcgccctgaa
    15301 ccgcttggct aaagaagacc cttctttccg tgtccgtaca gacgaagaat ccggtcaaac
    15361 cattatttcc ggtatgggtg agctgcactt ggaaattatt gttgaccgta tgaaacgcga
    15421 attcggtgtg gaagcaaata tcggtgcgcc tcaagtggct taccgtgaaa ctatccgcaa
    15481 agccgttaaa gccgaataca aacatgcaaa acaatccggt ggtaaaggtc aatacggtca
    15541 cgttgtgatt gaaatggaac ctatggaacc gggtggtgaa ggttacgagt ttatcgatga
    15601 aattaaaggt ggtgtgattc ctcgcgaatt tattccgtct gtcgataaag gtatccgcga
    15661 tacgttgcct aacggtatcg ttgccggcta tcctgtagtt gacgtacgta tccgtctggt
    15721 attcggttct taccatgatg tcgactcttc ccaattggca tttgaattgg ctgcttctca
    15781 agcgtttaaa gaaggtatgc gtcaagcatc tcccgccctg cttgagccga ttatggcagt
    15841 tgaagtggaa accccggaag aatacatggg cgacgtaatg ggcgacttga accgccgtcg
    15901 cggtgttgta ttgggtatgg atgatgacgg tatcggcggt aaaaaagtcc gtgccgaagt
    15961 acctctggca gaaatgttcg gttactcgac cgacctgcgt tctgcaaccc aaggccgcgc
    16021 tacttactct atggagttca agaaatattc tgaagctcct gctcacatag ctgctgctgt
    16081 aactgaagcc cgtaaaggct aatcaggcaa ataggccgtc cgaaaggctg aaatgatttt
    16141 tcagacggca ttgttcttta atcgatcttt aatgtaaagg aattagctca tggctaagga
    16201 aaaattcgaa cgtagcaaac cgcacgtaaa cgttggcacc atcggtcacg ttgaccatgg
    16261 taaaaccacc ctgactgccg ctttgactac tattttggct aaaaaattcg gtggtgctgc
    16321 aaaagcttac gaccaaatcg acaacgcacc cgaagaaaaa gcacgcggta ttaccattaa
    16381 cacctcgcac gtggaatacg aaaccgaaac ccgccactac gcacacgtag actgcccggg
    16441 gcacgccgac tacgttaaaa acatgattac cggcgccgca caaatggacg gtgcaatcct
    16501 ggtatgttcc gcagccgacg gccctatgcc gcaaacccgc gaacacatcc tgctggcccg
    16561 tcaagtaggc gtaccttaca tcatcgtgtt catgaacaaa tgcgacatgg tcgacgatgc
    16621 cgagctgttg gaactggttg aaatggaaat ccgcgacctg ctgtccagct acgacttccc
    16681 cggcgacgac tgcccgattg tacaaggttc tgcactgaaa gccttggaag gcgatgccgc
    16741 ttatgaagag aaaatcttcg aactggctgc cgcattggac agctacatcc cgactcccga
    16801 gcgtgccgtg gacaaaccgt tcctgctgcc tatcgaagac gtgttctcca tttccggccg
    16861 aggtacagta gtaaccggcc gtgtagagcg cggtatcatc cacgttggtg acgagatcga
    16921 aatcgtcggt ctgaaagaaa cccaaaaaac cacctgtacc ggtgttgaaa tgttccgcaa
    16981 actgctggac gaaggtcagg cgggcgacaa cgtaggcgta ttgctgcgcg gtaccaaacg
    17041 tgaagacgtg gaacgcggtc aggtattggc caaaccgggt actatcactc ctcacaccaa
    17101 attcaaagca gaagtgtacg tactgagcaa agaagagggc ggccgccata ctccgttctt
    17161 cgccaactac cgtccgcaat tctacttccg taccaccgac gtaaccggcg cggttacttt
    17221 ggaagaaggt gtggaaatgg taatgccggg tgagaacgta accattaccg tagaactgat
    17281 tgcgcctatc gctatggaag aaggcctgcg
//
LOCUS       DAWHXZ010000018        17130 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000018 DAWHXZ010000000
VERSION     DAWHXZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17130
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.018"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..325
                     /locus_tag="WIE35_000394"
     CDS             <1..325
                     /locus_tag="WIE35_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T cell stimulation protein TspA"
                     /protein_id="HEZ4153362.1"
                     /translation="TEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNN
                     ADTGFPSESVGSDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALA
                     QELGI"
     gene            complement(498..914)
                     /gene="gloA"
                     /locus_tag="WIE35_000395"
     CDS             complement(498..914)
                     /gene="gloA"
                     /locus_tag="WIE35_000395"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ4153363.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(939..2108)
                     /gene="lapB"
                     /locus_tag="WIE35_000396"
     CDS             complement(939..2108)
                     /gene="lapB"
                     /locus_tag="WIE35_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ4153364.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(2119..2433)
                     /locus_tag="WIE35_000397"
     CDS             complement(2119..2433)
                     /locus_tag="WIE35_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ4153365.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKVDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            2743..3741
                     /gene="ilvE"
                     /locus_tag="WIE35_000398"
     CDS             2743..3741
                     /gene="ilvE"
                     /locus_tag="WIE35_000398"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ4153366.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYSSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALIE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(3926..4711)
                     /gene="fabI"
                     /locus_tag="WIE35_000399"
     CDS             complement(3926..4711)
                     /gene="fabI"
                     /locus_tag="WIE35_000399"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ4153367.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELGSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(4866..5687)
                     /gene="dapD"
                     /locus_tag="WIE35_000400"
     CDS             complement(4866..5687)
                     /gene="dapD"
                     /locus_tag="WIE35_000400"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ4153368.1"
                     /translation="MSLQNIIETAFENRADINPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEILNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(5854..7497)
                     /gene="pgi"
                     /locus_tag="WIE35_000401"
     CDS             complement(5854..7497)
                     /gene="pgi"
                     /locus_tag="WIE35_000401"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ4153369.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALEFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHITVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPVWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            8007..9239
                     /gene="pilG"
                     /locus_tag="WIE35_000402"
     CDS             8007..9239
                     /gene="pilG"
                     /locus_tag="WIE35_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ4153370.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            9313..10173
                     /locus_tag="WIE35_000403"
     CDS             9313..10173
                     /locus_tag="WIE35_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ4153371.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            10175..10807
                     /locus_tag="WIE35_000404"
     CDS             10175..10807
                     /locus_tag="WIE35_000404"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ4153372.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            10800..11009
                     /locus_tag="WIE35_000405"
     CDS             10800..11009
                     /locus_tag="WIE35_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ4153373.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(11094..12770)
                     /gene="pilB"
                     /locus_tag="WIE35_000406"
     CDS             complement(11094..12770)
                     /gene="pilB"
                     /locus_tag="WIE35_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ4153374.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYKESQAGKEVLPILFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
     gene            complement(13169..13240)
                     /locus_tag="WIE35_000407"
     tRNA            complement(13169..13240)
                     /locus_tag="WIE35_000407"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13205..13207),aa:Arg,seq:cct)
     gene            complement(13260..13706)
                     /locus_tag="WIE35_000408"
     CDS             complement(13260..13706)
                     /locus_tag="WIE35_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ4153375.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(13711..14685)
                     /gene="ispB"
                     /locus_tag="WIE35_000409"
     CDS             complement(13711..14685)
                     /gene="ispB"
                     /locus_tag="WIE35_000409"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ4153376.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(14701..14889)
                     /locus_tag="WIE35_000410"
     CDS             complement(14701..14889)
                     /locus_tag="WIE35_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153377.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            14909..15217
                     /gene="rplU"
                     /locus_tag="WIE35_000411"
     CDS             14909..15217
                     /gene="rplU"
                     /locus_tag="WIE35_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ4153378.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            15242..15514
                     /gene="rpmA"
                     /locus_tag="WIE35_000412"
     CDS             15242..15514
                     /gene="rpmA"
                     /locus_tag="WIE35_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ4153379.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            15902..17086
                     /gene="ubiM"
                     /locus_tag="WIE35_000413"
     CDS             15902..17086
                     /gene="ubiM"
                     /locus_tag="WIE35_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ4153380.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTAVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGASSL
                     LEKYSNKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
BASE COUNT         4166 a         4743 c         4302 g         3919 t
ORIGIN      
        1 aacggaaatc actttggaaa cgcctgattc caacacctct gaggcagacg ctttgcccga
       61 cttcctgaaa gacggcgagg aggaaacggt agattggagc atctacctct cggaagaaaa
      121 tatcccaaat aatgcagata ccggtttccc ttcggaatct gtaggttctg acgcgccttc
      181 cgaagcgaaa tacgaccttg ccgaaatgta tctcgaaatc ggcgaccgcg atgccgctgc
      241 cgagacagtg cagaaattgc tggaagaagc ggaaggcgac gtactcaaac gtgcccaagc
      301 attggcgcag gaattgggta tttgattccc aactgccctt tcgcagatca aggatgccgt
      361 ttcagacggc atcttttttt gccttatcgg tgtaacggat aaagtttgaa ccggcacagg
      421 ctcaaacagc aggtcgacgg caacaaaatg ccgtctgaaa cccctaaagg cttcagacgg
      481 cattggcggc ggcggtatca ggcagtttga taggcaaccg aatcgtcgcc gcttttcttt
      541 tgaatgaact cgattttgta tccgtcgggg tcttcgacga aggctatcac ggttgtgccg
      601 tgtttcatcg ggccggcttc gcggacgacg tttccgccct gccgcttcac acgttcgcag
      661 gcttcgtagg catcgtccac ttcaaccgcg atgtgtccgt aggcgttgcc caagtcgtat
      721 cgttccgtat cccagttgtg cgtcagttcc aaaaccgtgc tgtcggtttc atcgccgtaa
      781 ccgacgaagg caagggtaaa tctgccttcg ggataatctt ttcggcggag cagtttcata
      841 cccaaaacgt tttggtagaa atcgagggat ttttcgagat tgcccacgcg gagcatagta
      901 tggagtaagc gcattttttg tgttcctttc ggtggtggtt aaacttcgat tttattcggg
      961 gtaaacgtct gccatttgtt gcaggcgggg cagtgccaga aaaagacttg tgatttgaag
     1021 tggcagtttc ggcaccggta catcacgctg cgctgtagct gccgtccgat aaccgaacgc
     1081 atcatatcgg catcggcttt ccaagccgga tccaaatcgc tgagtttcaa accaagcagg
     1141 cggtacacgc cgttgaggtc gggcttgcgg cggacaagct cgacggcggt ttgcgcggct
     1201 tctttctcgc acttaagcag cagggatttc tcgtacacga cattgatcag gtcaagttcg
     1261 ggaaacgtct gcatatatcc tgtcagacgg ttcaagcctt cttcaggttt tccctgcgcg
     1321 gcataggctt cgtaaagctt ctcgccgacc atgctcaagt atgcatggtt ttgctgctcg
     1381 atggcggcat aggcttcgac ggcggcaggg aaattgcctt gtcggtgttc gatgtcgccc
     1441 aaaatcatgt tggcacgggt gcattttttg ttggcttcga gtgccttgcc gacattgaaa
     1501 cgcgcgacat cgaaattgga cttgaacagc gcggcttggg caagttcgca ataaaactgg
     1561 gcgatttcaa actgataggt ctgatcgtca tggctgagca gccgggcggt ttcaaccgct
     1621 ttttcccaat ccctgtcctg ttggtagata ttgagcaggt gctgtctggc ttcacgcgcc
     1681 attttaccgt cttgcagccc caaaaaaatc tgttcggcac gatcgaccaa ccccgcactt
     1741 tggtagtttt gcgccaattc aaacaggacg cgcgcgcgct tttcgccgac cgtatcggga
     1801 gaatcgagca ttgtccggtg tatgttgatg gctttgtcgt tttcgccacg ctgacggtaa
     1861 agcttgccta gggtgaggtt taggtcgtat gactgaggct gctgatcaat gacctctgcc
     1921 aattccctgg cggcacgtcc gctgttgcgg tcaaccaagg catccagact tttataaaat
     1981 cccgacggta tgctttttgc ctgctttaat acagtcttca tatccacacg ggcggcaaac
     2041 cagcccatcg cgaagaaaac gggcaaaagg ataatcggca gcaggataat ccacaattca
     2101 ttgtccatat cggctttctc aaggctgttt ggcagattcg ggcgcatttt gcgccggtgg
     2161 tgcggtcagc tctttccccg tcaaacgcgc atttttcttt acttcggcac gcaacctgcc
     2221 gttctcgcca cgtaacgaca acaaccgtcc gaacaaggca aacattccga acacgatgcc
     2281 gacgacaaac gcgccgaaca atacgacaat cagcggcaaa tcgactttct gtccgggcag
     2341 gtaggagaag gtaacggcat ccgtattaat gacggcaagc agcaggaaga gcagcaggat
     2401 aatgattttg atgacggtat agataagttt catgtttgct ccgtaaataa aaacgccgcc
     2461 cggacagctt cgcactctga accaagattg cgctagttta aacgatttgc acggtttcgg
     2521 ataggatgcg gcagcgtgtt cggacgacat acggagtatg cgattgccga caattttacc
     2581 aaatacaccg ttcctttcca tttgaaaaat aacggattgg acaccgcatc gacagaaaaa
     2641 cccgccgcgc acttgtcaaa accctgtttg caggcgtatc tttacaatct tcaaattcaa
     2701 accgttcatt gaaacatatc agaataagaa aggctttaca tcatgagcag acccgtaccc
     2761 gccgtattcg gcagcgtttt tcacagtcaa atgcccgtcc tcgcctaccg cgaaggcaaa
     2821 tggcagccga ccgaatggca atcttcccaa gacctctccc tcgcaccggg tgcgcacgcc
     2881 ctgcactaca gcagcgaatg tttcgaggga ctgaaagcct tccgtcaggc agacggcaaa
     2941 atcgtactgt tccgtccgac tgccaacatc gcgcgtatgc ggcaaagtgc ggacattttg
     3001 cacctgccgc gccccgaaac ccaagcctat ctcgacgcgc taatcgaatt ggtcaaacgc
     3061 gccgccgatg aaattcccga tgcgcctgcc gccctgtacc tgcgtccgac cttaatcggt
     3121 accgatcccg tcatcggcaa ggccggttct ccttccgaaa ccgccctgct gtatattttg
     3181 gcttcccccg tcggcgacta tttcaaagtc ggctcgcccg tcaaaatttt agtggaaacc
     3241 gaacacatcc gctgcgcccc gcatatgggc cgcgtcaaat gcggcggcaa ctacgcttcc
     3301 gccatgcact gggtgctgaa ggcgaaagcc gaatacggcg caaaccaagt cctcttctgc
     3361 ccgaacggcg acgtgcagga aaccggcgcg tccaacttta tcctgattaa cggcgatgaa
     3421 atcattacca aaccgctgac cgacgaattt ttgcacggcg taacccgcga ttccgtactg
     3481 acggttgcca aagatttggg ctataccgtc agcgaacgca atttcacggt tgacgaactc
     3541 aaagctgcgg tggaaaacgg tgcggaggcg attctgaccg gtacggcagc cgtcatctcg
     3601 cccgttactt ccttcgtcat cggcggcaaa gaaatcgaag tgaaaagcca agaacgcggc
     3661 tatgccatcc gtaaggcgat taccgacatc cagtatggtt tggcggaaga caaatacggc
     3721 tggctggttg aagtgtgctg atgctttaaa taaaaaaatg ccgtctgaaa cccgtttggc
     3781 gtttcagacg gcattttcgc gttcggacca tttccgctgc acctgcagca agtcgggcat
     3841 ttccctagaa atacgcttca gacggcaaac cgccgcccga aaccgatacg cggtacggac
     3901 ggcgcacgga tttgaaaacg gcggattatc cctcggtgct caaggcatta atgctgtaac
     3961 cgccgtcaac gtaagtgatt tcgccggtaa tgccggacga caggtcggac agcaggaagg
     4021 cggcggtatt gccgacttct tcaatggtaa cgttgcggcg cagcggattg tgggcggcga
     4081 cgtgtcccaa aagtttgccg aaatcggcga taccggaggc ggcaagcgtt ttaatcgggc
     4141 cggcggaaat gccgttgcag cggatgcctt ctttgcccag acaggcggcg gtaaaacgga
     4201 tgcccgcctc aaggctggct tttgccatac ccatcacgtt gtaatttgga atcgcgcgca
     4261 ccgcgcccaa gtagctcagg gcaacgatgg cggagtttct gccgcgcatc atcggacggg
     4321 cggcttttgc caatgcgggc aggctgtatg cggaaatttc gtgtgcggtg ttgaacgctt
     4381 cgcggctgat gctgtcgagg aagtcgccgc tcaaggcttc tttcggcgcg aagccgatgg
     4441 aatgcaccaa accgtccaag ccgtcccaat gtttgcccaa gtcggcgaac acttggttga
     4501 tttcgtcgtc gctggcgaca tcgcagcgga atacgagttc ggaacccaat tccgccgcca
     4561 ttttgcggac gcgctcttcc agtttgtcca caacgtaggt aaacgccagt tccgcgcctt
     4621 gttcgcggca ggctttggcg atgccataag cgatggaacg ctcggaaatc atgccggtaa
     4681 tcagaatttt tttgccttgc agaaaaccca ttttgtatcc ttcaaatagt gttcggttca
     4741 ttctaaaccg ttgattatag caaattgtcc ctgtttctgt gttttcacgt tgcagcatgc
     4801 aaacggcaat gccgtccgaa gcggatttca gacggcatcg gacgtttcaa atacggttta
     4861 aggcatcaga tgccgcgcaa caattcgttc acgctggttt tcgcacgggt ttgcgcgtcc
     4921 acgcgtttga cgatgacggc gcagtaaagg ctgtggctgc cgtctttgga aggcatactg
     4981 ccggacacga cgaccgaacc ggccggtacg cggccttggt agatttcgcc ggtggtgcgg
     5041 tcgaagattt tggtggattg accgatgaac acgcccatag aaatcacgct gccttcttcg
     5101 acaatcacgc cctcaacgat ttcggaacgc gcaccgatga agcaattgtc ttcaataatg
     5161 gttgggctgg cttgcagggg ttcgagtacg ccgccgatgc cgacaccgcc gctcaagtgg
     5221 acgtttttgc cgatttgcgc gcaagagccg acggtcgccc aagtatcgac catcgcacct
     5281 tcgtcaacgt atgcgccgat gttgacgtaa gacggcatca gcacgacatt tttcgccaca
     5341 aagctgccgc gtcgggcaac cgcacccgga acggcgcgga agcctgcgtt tttgaactcg
     5401 tcttcagacc agtcggcaaa cttggtcggc actttgtcga agtatttgtt cacaccgtcg
     5461 ttgaggattt cgttgtcttg gatgcggaag gacagcaaca cggctttttt cgcccattcg
     5521 ttaactttcc attcgcccac gcccaagcgt tcggcgacgc gcagtttgcc ggaatcgagt
     5581 tggcggatgg tttccaacac ggcttcttta acttcgggag taacggtggt cggattaatg
     5641 tccgcgcggt tttcaaaggc ggtttcgata atgttttgca aagacataat gtttccttat
     5701 gtgagatgtt tcgtgggacg cttgccggta ttttcaagca gtatgttcat ttgaaatgaa
     5761 tgcgctattg tgccacgcaa acgcatattc gtcagcaata cgcgggcggt tgccgaacat
     5821 tggcgtttcg gaatcgggcg ggcggcagta cctttatgcc gcaccgccgc ctttcagacg
     5881 gcattcgcgg taaaacgcca tcagcccttc ggtcgaggca tcgtgtacgg acgtgccgcc
     5941 ttccagttcg ccgatgatgg tttttgccaa ctgtttgccg tattccaccc cccactgatc
     6001 gaaggggttg acgttccata tcgcgccttg gacgaaggtt ttgtgttcgt aagccgccat
     6061 cagcataccc aaattgtagg gcgtgaggcg gtcgagcagg atgctgttgc tggggcggtt
     6121 gccagggaac tctttgtgcg gcgcgaggcg ttcgcgttcc gcttcgggca aatctgccag
     6181 ttcggcgcgt gcttcgtcca aggttttgcc cttcatcagg gcttccgctt gggcaaaggc
     6241 gttggcaacg gtaaaacggc tgcgtccgtc ctccacgccc tgcgccgtca tcgggacgat
     6301 aaagtcgcag ggaatcaggc gcgtgccttg atggagcagt tggaaatagg cgtgctggca
     6361 gttgacccct tcaccgccga acacgatgcc gcccgttttg cacacggcgg gactgccgtc
     6421 tgaagcgcgg cttttgccca aactctccat atcgagctgg ttcagccaca ccggcagcag
     6481 gcgcaggttg tggctgtacg gaacggcggt ctgcccgtcc gcgtgttgga aattgttata
     6541 ccacacggca atcagtgcca tcagaacggg aatgttgcgg cgcggcggcg tgtggaaaaa
     6601 atggctgtcc atcgcgtgcg cccccgccaa caactcgcgg aaacgcgccc cgccgaccgc
     6661 aaccatcacg ggcaaaccga cgggcgacca gacggaatag cgtccgccca cccagtcgta
     6721 catcgcaaac acgcgttccg ccgcgatacc aaaagccgca gctgccgcag tgtcggcaga
     6781 caccgcgcaa aaatggcacg ccgtttcgga ttccgagaac cccgcaccgc gataccacgc
     6841 cttgactgcc tgtgcattga gcagggtttc cggtgttttg aaggacttgc tggcaacgca
     6901 aaacactgtc gtttcggggt tcagacggca taaaaccgca tccaggcagg caggatcggc
     6961 gttggcggca aaatggacgg tgatatgccg tctgaacggc tcaagtgcct gcacgcacat
     7021 tgccggcccg aggtcggatc cgcctatgcc gatgtggaca aaatccgtaa tccgttttcc
     7081 ggttgtcccc tgatacgaac cgtcgtccaa actgtgtgca aactccaacg cacgatttaa
     7141 ctcgcggcgg atttcgggca acacgtccct gccgtccgca taaacggcat ccgcaccgtc
     7201 gggtaggcgc aaagccgtat gcagcgcggc acgcccctcg ctgccgttga ctttcgcacc
     7261 cgtccgcaaa gcacgcattt tcccttccaa atccgccgtc tccgcaagac ggcagagcag
     7321 ttgcagcgta tcttcgccca aacggttttt gctgtaatcg aacaacatcc cgtccaaacg
     7381 ctcatgcata cgctcaaaac ggtcgggttc gcaggcaaag cggtcgcgca aaaggacatg
     7441 acgcgtatcc tgataatggc gttcgagcgc ataccatgca cgggtaaaag cattcatctg
     7501 ttttccttga tttttcagaa ccggattaaa atgtagcaga atgtagttta acaaacggca
     7561 gcggctttgg cgaatctccg gaacaccgcc cgccgcgtcc aatagcacct tatttccata
     7621 tggcgaaaat atagtgaatt aacaaaaatc aggacaaggc ggcgagccgc aaacagtaca
     7681 aatagtacgg aaccgattca cttggtgctt cagcacctta gagaatcgtt ctctcttgag
     7741 ctaaggcgag ccaacgctgt accggttttt gttaattcac tataccctcc ggccggcatt
     7801 tgacccgcaa caatatcctg caagatttat tgtgtacgca taaatgccgg acagccgcct
     7861 aaataaggta taatcgcatc cgattctgtc ccgtttgccg cccgcaggcg ggatgtcggc
     7921 ggttttcatc ttaccgggaa cacgccggta ctgtttttta acgatgtttc ttacatttta
     7981 ttccaattac tttacggggc tagaatatgg ctaaaaacgg aggattttct ttgttcgcaa
     8041 agaaagaaaa acgctttatc tttgaaggca ggcattccgc ctccgacaaa ctggtcaacg
     8101 gcgaagtatc cgcgtttacc gaagaagagg cgcgcaaaaa actggcaaaa cgcggcatcc
     8161 gcccgttgca gattacccgt gtgaaaacaa gctccaagcg caaaatcaca caagaagaca
     8221 tcaccgtttt cacccgccag ctgtccacga tgattaaagc gggcctgccg ctgatgcagg
     8281 catttgaaat cgtggcgcgc ggacacggca acccgtctat gacggaaatg ctgatggaaa
     8341 tccgaggcga agtggaacag ggcagctcgt tgagccgcgc attctcaaac cacccaaaat
     8401 atttcgaccg cttctactgc aatctggttg cggcgggcga aacgggcggc gtattggaaa
     8461 gcctgctgga caaattggca atttacaaag aaaaaaccca ggccatccgc aaaaaggtaa
     8521 aaaccgcact gacctatccg gtatccgtca tcgccgtcgc catcggtttg gtattcgtga
     8581 tgatgatttt cgtactgccc gcctttaaag aagtttacgc caatatgggc gcggagcttc
     8641 ccgcactgac ccaaacagtg atggatatgt ccgacttttt cgtctcatac ggctggatgg
     8701 tgctgatcgc actgggcttt gccatatacg gcttccttaa attgaaggcg cgttcgatta
     8761 aaatccaacg gcgtatggat gccatactgc tgcgtatgcc gattttcgga gacattgtcc
     8821 gcaaagggac gattgcccgc tggggcagga cgacggcgac gctgattgcg gcaggcgtgc
     8881 ctttggtcga tgtattggac tccactgccg gcgcggcggg caatttaatc tatgaagaag
     8941 ccacccggga aatccgtacg cgcgtcatcc aaggtttgtc tatgacttcg gggatgcgtg
     9001 cgacggaact gttccccaat atgatgttgc agatgtcttc catcggcgag gaatcgggtt
     9061 ctttggacga tatgctcaac aaagccgccg aattttacga agacgaggtg gacaatgcgg
     9121 tcggcaggct gtccgctatg atggagccga tcattatcgt gattttaggc ttggtcatcg
     9181 gcacgcttct ggtcgccatg tatctgccgc tgtttaattt gggcaacgtg gtcgcctgat
     9241 ttgccgcaca gatccggcgc ggattcgctc tgcgccggtt tgtttttgtt ttgaatacat
     9301 tgagaacaaa atatgtctga tttgtctgta ttgtcgccgt ttgccgtgcc tttggcagcg
     9361 gtgttcgggc tgctggtcgg cagtttctta aacgtcgtca tttaccgcgt gccggtcatg
     9421 atggaacgcg gctggacggt atttgccaaa gaatatttaa acctgccgct gaccgaagag
     9481 gaaagccgta ccttcaacct gatgaaaccg gattcctgct gtcccaaatg ccgcgtgccg
     9541 atacgcgcgt ggcagaacat cccgattgtc agttacctgc tcctgcgcgg caaatgcgcc
     9601 tcctgtcaaa ccaaaatcag catacgttat cccttaatcg agctgctgac cggcgtattg
     9661 ttcgggctgg tcgcctggca atacggctgg tcttggatta cgctgggcgg attggtactg
     9721 accgcgtttc tgatttccct gacctttatc gatgcggaca cccaatacct gcccgactcg
     9781 atgacactgc ccttgatttg gctggggctg atatttaatt tggacggcgg cttcgtgcct
     9841 ttgcagtctg ccgttttagg tgcggttgcc ggctatggtt cgatatggct tttatgcgcg
     9901 gtatataaac tgattaaagg cgaaatcggt atgggcaacg gagatttcaa actgattgcc
     9961 gcattgggcg cgtggctcgg catatccgca ttgcccgtgc tgatttttgt ttcctcgctg
    10021 atcggtttgg tcgcggcaat cgttatgcgc gtcgccaagg ggcagcattt tgccttcggc
    10081 cccgcactga cagtttcggg ctggataatt tttacggcaa acgattccgt atggcgggcg
    10141 gtcaactggt ggctgaccca tccggtgctg taagatgacg gtatgggtcg gactgaccgg
    10201 cggaatcggc agcggcaaat cggcagccgc gcaatgtttt gccgatttgg gcgtgccgcg
    10261 catcgatgcg gacgcggcgg cgcactcgct gacggcttca gacggcatcg ccctgccgga
    10321 aatcaggcgg ctgttcggcg acaccgtttt cgacacacag ggtttgttgc gacgcgacat
    10381 attgcgtaaa gaagtctttg cctccccatc gcgaaaagcc ttgctcgaat ccgtgatgtt
    10441 gccgctgatt ttctcagaaa tcaaaaaaca gcaagaaacc tttaccgatg cggcttacgg
    10501 cattgtcgaa attccgctgc tgacggaaaa gcgtcaattt atcagcctga tacggcgtgt
    10561 cctgaccata agcgcacctt tggaaaaacg tatcggcagg gtgatggccc gcagcgggct
    10621 gacgcgcggc gaggtggcgg acatcatcag ccatcaggca tccgaatccg aacgcctgct
    10681 gcttgcagac gatgtgctgc tcaatgacgg cagcctcaaa agcctgcgtg agaaaacaat
    10741 gcgcctgcac gcgttttatt cagggatttt cgcctcaaaa ccaacacaag gaaaacacaa
    10801 tgactgaatc gcggcaaaca cgccttcaag tcaaatgtcc gacctgtcaa acagcagtag
    10861 tatgggaacc cgaaaacgca ttccgcccct tctgttcgca acgctgcaaa ctgatcgact
    10921 tgggcggatg ggcagacggg aaatatacgg tttccggcca aacggaaagt ttgccggaaa
    10981 tatccgaacc cgacatggca taccgctgac cgccccgcct tcccggcaaa caccctgaaa
    11041 gtcaaatgcc gtctgaaaca aacacgcttc agacggcatt ttcattctca aacctaatcg
    11101 ttggtatttg ccgttacctc ttccaatgaa gtaatgccct gcataacttt caaaataccg
    11161 gcccggcgca aatccaccat accctcctta taggcaacgt ccaaaatatc cacttccgta
    11221 ccgttgttca taatcacacg ctgcatttct tcgctgatgg gcataacctc atacacgccc
    11281 gcacgcccct tataaccctg cccccggcaa cggtcgcaac cgacggcgcg gtaaagtttc
    11341 caatcttttg caaggtcctc atcggtgaag ccgacttcct tcaaagcaga ggcagacggg
    11401 cgttccactt cctgtttgca gctcgaacac agcctgcgta aaagacgctg cgccataatc
    11461 aggctgaccg aactggcaat attaaacggc gcgacaccca tattcagcat acgcgacaac
    11521 gtcgccggcg cattattggt gtgcagggtg gaaaacacca tatgccctgt ttgtgccgcc
    11581 ttaatcgcaa tatcggcagt ttccaaatca cgaatctcac cgaccataat gatgtccggg
    11641 tcctgacgca ggaaagactt caaagcagcg gcaaaagtca ggccctgctt atcattgacg
    11701 ttaacctgat tgatgcccgg caggttaatc tcggcagggt cttccgccgt tgcaatattt
    11761 accgactccg tattcaaaat attcaaacag gtatagagcg acaccgtctt acccgaaccc
    11821 gtcggaccgg ttaccagcac catcccgtag ggacggtgaa tcgcttccaa caacaatttt
    11881 ttctgaaacg gctcaaaacc gagctggtcg atgttcaaag acgcggcatc ggaattcaaa
    11941 atccgcatca cgaccttttc gccaaacagc gtcggcaatg tgctgacacg gaaatcgaca
    12001 ggcttgccgc ccttttgaaa ggtcagctgc atcctgccgt cctgcggtat ccgtttttcg
    12061 gaaatgtcca aacgcgacat taccttaatc cgggaagcaa gctgccccct taccgcaatg
    12121 ggcggctgaa ccacctcgcg gagctgcccg tccacacgga aacggatacg ggcattgtgt
    12181 tcgtaaaact cgaaatggat gtccgatgcc ccgctgcgca aggcatccga caaagtctta
    12241 tggataaacc tcggaacagg gccgtcttct gcctcctcgt tgtcgatata cagggtgtgg
    12301 ctttcctctt cctcctgccc ctccccaagc tcctgaagca gcgatgtcga acgcgaaccc
    12361 acccaatcga gcaaacccgc caactggtca tcctcgacaa tgaccaactc aacctcaatc
    12421 cctgcggcag aaacggtttt ctgaatttgc ggcatctgtg tcggatcgga aaccgcaaaa
    12481 aatactttgt cgccccgacg gaaaaccggc acacagtgga actccaccat ctgctcctcc
    12541 gtcaacaccc ccatcagcac cctgtggcgc ggataatgac gcaaatcaag aatcgaataa
    12601 ctgaacaccc ttgcaatcaa tgccgcaagc gacttgggcg aaatgacgcc gtctgaaaac
    12661 agtatcggca acacttcctt acccgcctgc gactccttgt agtaatgctc ggcctgctca
    12721 acagtaacca cctggttttg aaccagaatc ctcagcaaac ctacgctcat acgaccttat
    12781 ccccaaattt attcattgtt atacctgtac agcttttata gtggattaaa tttaaaccag
    12841 tacggcgttg cctcgcctta gctcaaagag aacgattctc taaggtgctg aagcaccaag
    12901 tgaatcggtt ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
    12961 taatccacta tatcataaaa caacagattt ccaagccgga acatctttta cggagcctga
    13021 aaatcatttc atgattctac cgtcctaaag gtcggttttt caagcaggaa gaaaaagttt
    13081 tcagatggca aaaaagccct ccaacactga aaggccttat atcggaaact tcccgcaaca
    13141 cgggaaacag acaaatgaaa tcgtcaaacc tcgccaacag gaatcgaacc tgtattttac
    13201 gcttaggagg catacgttct atccgttgaa ctatggcgag ccgaaatgaa aaggagattt
    13261 taaccctttc cgacgacaaa agacaagatg ccggccgcaa tcagcggccc gacagggata
    13321 ccgcccataa atgccacccc gataaccgtc ccgattaaca gccctgtaat taaaacaggc
    13381 tgctgtccca tcaaaggcac gccgcgtccc gccagccaag ccacgaaaat accgataaaa
    13441 acggcggata tcattttaaa attcaaaaat tcggcaacgg gaggaacctg cgcctttcct
    13501 gaaaccaacg gactcaaaac ccctatggtc aaaagaatga taccgagatt caacccatgc
    13561 ttctcgacca acgggacaaa ctgtaccaat gccgtctgct gcatcagcag caatatggtt
    13621 gccgagatgg taatcgaatt gttgttgctg accaccccca acagaatcag cgtaaccaga
    13681 aacaggggaa caaaactgaa attcatcgcc tcaagacacc ctgaccaaag attccttcgc
    13741 cagctgaatc atggcatcct tcacttcgct gtcgggcagg gcatccaagg cggtaacggc
    13801 acaatcgact gctttgcgcg cctcgcctat cgaatatgcc aaagcatccg aacggacgac
    13861 ataatcgtgg attttctcaa aatagctgcg atctgcattt tccaaagcag tacgcacatc
    13921 gttcgcaacc tgttcggaac cctgacgcat cagataaatc aaaggcaaag tcggttttcc
    13981 ttccgccaaa tcgtcgccga cgtttttgcc ggtttcttcg gtttcgccag aatagtccag
    14041 cacatcgtca ataatttgga atgccgtacc gacatacata ccgtagtctt tcaacgcccg
    14101 ttcgtgttcg ggggaagcct tgcccaaaat tgcgccgacc tgcgccgccg cttcaaacaa
    14161 ttttgccgtt ttatattgga tgacttggat atattgttct tcggtaatgt ccgtattgcc
    14221 gatgttcatc agctgcatga cttcgccctc ggcaataatg ttggttgcat ccgccatcac
    14281 ttccaaaacg cgcatactgc ccgaggcaac catcagttga aaggcgcgcg tgtataaaaa
    14341 gtcgccaacc aacacagccg ccgcattgcc gaacagattg tttgccgttg cccgcccacg
    14401 gcgcaaatcg ctttcatcga cgacatcgtc gtgcagaagc gtcgaagtgt ggataaactc
    14461 gaccatcgcc gccagcgaat acagtttctc gtcatcataa ccgaccgcct tacccgccaa
    14521 aatcgtcata atcggacgca ggcgtttgcc gcccgcgctg atgatatatg taccgatttg
    14581 cgaaatcagt gcgacatcgg attgcaccgc acggttgatg acttcattga ctttggcaag
    14641 gtcttcaggc agatgtcgct ggaaataggg cagattctcg agcatggcag actctcaggt
    14701 ttacgtttcc gtcaaacggc agataccgct tgaaagctga ttcaaatagc gggcaattat
    14761 atcagcaaag ccatcagggt acatcttttc agacggcata tggttatccg aaccgcgcaa
    14821 actttgacaa aacaggcaaa taacaataca atgccgagtt ccgcacaatg gtgtgcggat
    14881 attaaccata acccttatgg agttgagtat gtacgcggtc gtaaaaactg gcggcaaaca
    14941 gtataaagtt tccgtcggcg aaaaattgaa agtagaacag ataccagccg aactcgacag
    15001 ccaaatcgaa ctgaccgaag ttttgatgat tgctgacggc gaatctgtaa aagttggcgc
    15061 accctttatc gaaggtgcaa aagtaacggc taaagtagtg gcacacggtc gtggcgaaaa
    15121 agtccgcatc ttcaaaatgc gccgccgcaa acactaccaa aaacgccaag gccaccgcca
    15181 aaatttcacc caaatcgaaa tcgtggcaat cgcctaacct taaaacttag gagcattaca
    15241 aatggcaagt aaaaaagcag gcggcagcac ccgcaacggt cgcgattcag aagccaaacg
    15301 cttgggcgtt aaagcctacg gcaacgagct gattcccgca ggttccatca tcgtccgcca
    15361 acgcggcacc aaattccacg caggcgacaa cgtaggtatg ggcaaagacc acactttgtt
    15421 cgccaaagtt gacggttatg tcgaattcaa aaccaaaggc gcgctgaacc gcaaaaccgt
    15481 aagcatccgt ccttacaccg gttctgaaga ataattcaac cgaacaggaa gccgcatctt
    15541 ttcacgatgc ggctttttat agtggattaa caaaaatcag gacaaggcga cgaagccgca
    15601 gacagtacaa atagtacgga accgattcac ttggtgcttc agcaccttag agaatcgttc
    15661 tctttgagct aaggcgaggc aacgccgtac tggtttaaat ttaatccact atacataccc
    15721 cacacccgat ttgatacaca ttaagctgaa agtaaaaatc cgcatacacc ctcccctgca
    15781 tatttcttca acagtgggtt ttgatataat cgcctatctg ttacagatag ttcaaacggc
    15841 attcaaaccc ttacaaatac cgtctgaaat catcggcatc cgtctatgca aaggatattt
    15901 tatgagttta cacagcgaca tcctcgtcgt cggcgcaggc ccggcgggtt taagttttgc
    15961 agccgaactt gccggcagcg gtttgaaagt taccctgatc gaacgcagcc cactgactgt
    16021 tctgcaaaat cctccatatg acggacgcga aatcgccctg acccattttt cccgcgaaat
    16081 catgcagcgt ttgggtatgt gggacaaaat tcccgaaaac gaaatctacc ctttgcgcga
    16141 tgccaaagtg ctgaacggac gttccgacta ccagctccac tttccccaac cgaccgaggc
    16201 gcgcggcgag cctgccgact gtttgggcta tctgatttcc aaccacaata tccgccgcgc
    16261 tgcctacgaa gtcgtatccc aactcgacaa cattaccatt ctgaccgata cggccgttaa
    16321 agaagtcaaa acatctgaca acgaagcaca agtcatttta gaaaacggca aaatcctgac
    16381 cgcccgcctg cttttggcgg cagacagccg tttttcgcaa acccgccgac aactcggcat
    16441 ttcttcagat atgcacgact acagccgaac catgttcgtc tgccgcatga aacatactct
    16501 ttccaaccaa cataccgctt acgaatgctt ccattacggg cgcaccatcg cactgctgcc
    16561 gctggaagaa cacctgacca ataccgtcat taccgtcgat accgataaaa tcaacagcgt
    16621 tcaaaacctt tcgcccgaag aattggcggc aagcgtaaaa gaacagctca agggcaggct
    16681 cggcgatatg gaattggtca gcagcatcca ccattacccc cttgtcggta tgattgccaa
    16741 acgtttctac ggcaaacgca gcgcgctgat tggcgatgcg gcggtcggta tgcaccctgt
    16801 aacggcacac ggcttcaact tgggtctttc cagcgcagat attctggcaa aactgatact
    16861 cgaagccgaa caacgcggtc aggacatcgg cgcatcttcc ttgttggaaa aatacagcaa
    16921 caaacatatg ctgcacgccc atccgcttta tcacggaacc aatatgatgc tgaaactgtt
    16981 taccaacgaa accgcgccgg caaaactgct gcgcggtttg gtattgcgtg ccggcaacaa
    17041 cttcccgccg ctgaaaaagc tgattaccaa acaactgacc ggttgaaaac tgccgaaatg
    17101 ccgtctgaaa cgtttcagac ggcatttttg
//
LOCUS       DAWHXZ010000019        16665 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000019 DAWHXZ010000000
VERSION     DAWHXZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16665
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.019"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            30..917
                     /gene="prmA"
                     /locus_tag="WIE35_000414"
     CDS             30..917
                     /gene="prmA"
                     /locus_tag="WIE35_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ4153381.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQIEELSGIYSQWF
                     DLDPAETEEGWARLSGTKR"
     gene            935..1498
                     /gene="orn"
                     /locus_tag="WIE35_000415"
     CDS             935..1498
                     /gene="orn"
                     /locus_tag="WIE35_000415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ4153382.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPERATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            1509..1781
                     /locus_tag="WIE35_000416"
     CDS             1509..1781
                     /locus_tag="WIE35_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HEZ4153383.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(1997..3280)
                     /gene="hemL"
                     /locus_tag="WIE35_000417"
     CDS             complement(1997..3280)
                     /gene="hemL"
                     /locus_tag="WIE35_000417"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ4153384.1"
                     /translation="MNRNEILFDRAKAVIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIVIAEQIAEI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FQTAADAAGIEFTADSVGGMFGLYFASHTPQNYGDMARSNIEGFKQFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(3344..3517)
                     /locus_tag="WIE35_000418"
     CDS             complement(3344..3517)
                     /locus_tag="WIE35_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ4153385.1"
                     /translation="MKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
     gene            complement(3830..3910)
                     /locus_tag="WIE35_000419"
                     /pseudo
     CDS             complement(3830..3910)
                     /locus_tag="WIE35_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            4113..5441
                     /gene="miaB"
                     /locus_tag="WIE35_000420"
     CDS             4113..5441
                     /gene="miaB"
                     /locus_tag="WIE35_000420"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ4153386.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(5526..7439)
                     /gene="dxs"
                     /locus_tag="WIE35_000421"
     CDS             complement(5526..7439)
                     /gene="dxs"
                     /locus_tag="WIE35_000421"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ4153387.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRTFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRSESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMELAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(7524..8441)
                     /gene="xerC"
                     /locus_tag="WIE35_000422"
     CDS             complement(7524..8441)
                     /gene="xerC"
                     /locus_tag="WIE35_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ4153388.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDEGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQPERVPKALSQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLVGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            8635..9699
                     /locus_tag="WIE35_000423"
     CDS             8635..9699
                     /locus_tag="WIE35_000423"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ4153389.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            9719..10678
                     /locus_tag="WIE35_000424"
     CDS             9719..10678
                     /locus_tag="WIE35_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ4153390.1"
                     /translation="MPSEPLFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTTPLDHKDKSLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQTITLASGE
                     FQIYKQNHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAFNQLPDGKAEYHG
                     KAFSSDDPNGRLHYSIDFTKKQGYGRIEHLKTPEQNVELASAELKADEKSHAVILGDT
                     RYGGEEKGTYHLALFGDRAQEIAGSATVKIREKVHEIGIAGKQ"
     gene            10817..11494
                     /gene="tsaB"
                     /locus_tag="WIE35_000425"
     CDS             10817..11494
                     /gene="tsaB"
                     /locus_tag="WIE35_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ4153391.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETLLFHQEAGSRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFNTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHSELLYVRNKIALTAKEQ
                     AERKART"
     gene            11491..11931
                     /gene="rimI"
                     /locus_tag="WIE35_000426"
     CDS             11491..11931
                     /gene="rimI"
                     /locus_tag="WIE35_000426"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ4153392.1"
                     /translation="MNIRRASPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFAHLPEDTQRLLLE
                     VRAGNTAAQALYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            11925..12662
                     /locus_tag="WIE35_000427"
     CDS             11925..12662
                     /locus_tag="WIE35_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ4153393.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFYGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            12724..13365
                     /gene="pyrE"
                     /locus_tag="WIE35_000428"
     CDS             12724..13365
                     /gene="pyrE"
                     /locus_tag="WIE35_000428"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ4153394.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            13439..13873
                     /locus_tag="WIE35_000429"
     CDS             13439..13873
                     /locus_tag="WIE35_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153395.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            13870..15180
                     /gene="argA"
                     /locus_tag="WIE35_000430"
     CDS             13870..15180
                     /gene="argA"
                     /locus_tag="WIE35_000430"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ4153396.1"
                     /translation="MIVPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFHLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AVTLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCATLKTFTEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            15276..>16665
                     /locus_tag="WIE35_000431"
     CDS             15276..>16665
                     /locus_tag="WIE35_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ4153397.1"
                     /translation="MKSYPDPYRHFEDLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILAQLQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWGERQLTESGYPREVWLVERGKSFEESLPVYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGEAKPLNLPNDCDVAGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLALFAL
                     DLNVMELTVMRRQPQQFDSDGINVRQFWAVSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGVPELPHYLGS"
BASE COUNT         3826 a         5101 c         4380 g         3358 t
ORIGIN      
        1 aatccactat atttccaaga aagcccgtta tgccctacca acaaatcacc gtcaacgtca
       61 acgatgccgt cgccgaacgc ctcgccgacg cgctgatgga acacggcgca ctctccgccg
      121 ccatcgaaga tgcctacgcc ggcacgcaaa acgaacaggc gattttcggc gaacccggta
      181 tgcccgccga acaaatctgg cagcagagca aagtcatcgc cctgttcggc gaacacgacg
      241 aagccgccgc catcatccaa atcgccgcac aagaatgcgg gttaaaagac ttggcataca
      301 ccggcgaaac catcgaagac caagactggg tgcgtctcac gcaatcgcaa ttcgacccca
      361 tccgaatttc cgaccgcctg tggattaccc cctcttggca cgaagccccc gaaggcactg
      421 ccgtcaacct acgcctcgat cccggcctcg ccttcggcac tggcagccac ccaaccacgc
      481 gcctctgcct caaatggctg gatacgcaac tcaaaaacgg cgaaagcgtc ctcgactacg
      541 gctgcggttc gggcatcctg accatcgctg ccctcaaact cggcgcaggt tctgccgtcg
      601 gcgtcgatat tgacgaacaa gccgtccgcg ccggcaagga caacgccgaa cagaacaacg
      661 tcgatgcaca attcttcctg cccgacggcc tgcctcaagg gcaattcgac gtagttgttg
      721 ccaacatcct cgccaaccct ttacgtatgc tcggcgaaat gcttgccgcc cgcaccaaac
      781 aaggcggccg catcgtgtta tccggtttgt tggacgaaca aatcgaagaa ctcagcggca
      841 tttacagcca atggttcgat ctcgatcctg cggaaaccga agaaggatgg gcgcgattaa
      901 gcgggacaaa acgctgacaa gcaaaggaaa catcatgcag gataaaaaca acctatgctg
      961 gctcgatatg gaaatgacgg ggcttaatcc cgaaaccgac cgcattatcg aagtcgcgat
     1021 gattattacc gactcggatt tgaatgtatt ggcgcaatcc gaagtttacg ccatccacca
     1081 aagcgacgag ctgctcgaca atatggacga gtggaacacc gccacacacg gtaggacggg
     1141 gctgacgcag cgcgtacgcg aatcgtcgca taccgaagcc gaagtcgaac agaaactgct
     1201 ggactttatg tcggaatggg tacccgaacg cgccacgccg atgtgcggca actccatcca
     1261 ccaagaccgg cgttttatgg tcaaatatat gccgaaactg gaaaactact tccactaccg
     1321 caacctcgac gtttccacgc tcaaagagct tgccaaacgc tggaatccgc ccgttgccaa
     1381 aagcgtggtc aaacgcggtt cgcacaaggc attggacgac attttggaga gcatcgaaga
     1441 aatgcgccac taccgcgaac actttctgat ttccgccccg agagccgaag cgcaataaga
     1501 aacaaacaat gccgtctgaa gcgcaaatcc tgcatttcag acggcatttt tacagcagat
     1561 tgaaatcaaa aatatacacg cccgtcattc ccgcgcaggc gggaatccgg aaggtcgggc
     1621 ttgttggtca tgaagtggtc ttgaaaatga aacgttatcg aaaatctgac gaactgaaag
     1681 aaaaatatag cgatatagat gagacttttg aatttataaa ggggtttatc tatgtaattg
     1741 cccttgcttt taccgcatat gtcgctatcc gttttttctg atgcgcttcc gataaaatgc
     1801 cgtctgaagg cttcagacgg cattgtttca ggtggcggcg gcttcctgcc gggtgcggct
     1861 gatttcggca cgttattttc aatcattaca gaaactgaaa ggtctggatt cccgcctgcg
     1921 cgggaatgac ggcggtgtgc attcttactt caatctgcca aacaaattga acagggaaac
     1981 cctgtccgtc aaaacatcat gcagccatcg ccttgaacac ttcaaccgca accgcaaccg
     2041 tttcatcaat cagctcgggc gtatgcgcgg cagaaacgaa acctgcttca taagcggacg
     2101 ggccgaaggc aatgccgcgg tcgagcatgc cgtggaaaaa ctgtttgaag ccttcaatat
     2161 tggaacgcgc catgtcgccg tagttttgcg gcgtatggct ggcaaaatac aaaccaaaca
     2221 taccgcccac gctgtcggcg gtgaactcga tgcccgccgc atccgctgcc gtctgaaaac
     2281 cttgaaccaa ctgttcggta cgcgccgtca ggttttcata aaacccttcg cggcggatga
     2341 tttccagcgt tttcaaaccg gcggcgacgg caatcgggtt gcctgataat gtacctgcct
     2401 gatacacgcc gcccagtggg gaaatacatt ccataatgtc tttgcgtccg ccgaacgcgg
     2461 caagcggcat accgccgccg atgactttgc ccatcgtggt caggtcgggc gtgatgccgt
     2521 gcagcgattg tgcgccaccg agtgcgacgc ggaaacctgt catcacttcg tcgtaaatca
     2581 acaccgcgcc gtgtttttca gtcaattcgc gcagggcttt gacgaaggct tcggtcgggc
     2641 ggacaaggtt catattgccg acaaaaggct ccaaaatcac acaggcaatg tcattgccgc
     2701 tttgagcaaa ggcttcttcc aattgggcga tgttgttgta ttcgaggact aaggtgtgtt
     2761 tggtaaagtc ggcaggcaca ccggcggaag aaggattgcc gaacgtcagc aggccgctgc
     2821 cggctttcac cagcaggctg tcggaatgcc cgtggtagca gccttcaaac ttgatgattt
     2881 tgtcgcggcc ggtaaaaccg cgtgccagac ggatggcggt catcgtcgct tccgtgccgg
     2941 agctgacgag gcgcagccgt tcgacagacg gcataatttc ggcgatttgt tcggcgatga
     3001 cgatttcgcc ctcggtgggc gcaccgaatg acaaaccgcc caacgcggct tcgcgcacgg
     3061 cttcgacgac ttcggggtgc gcgtgtccga caatcgcagg gccccaagag ccgacatagt
     3121 cggtgtagcg cgtgccgttt tcatcccaaa catacgcgcc ttcggctttt ttgatgaagc
     3181 gcggtacgcc gccgacgctg ccgaatgcgc gcacggggga attcacgccg ccggggatga
     3241 ccgccttggc gcggtcgaat aaaatttcgt tacggttcat atttatcctc aaatgccgtc
     3301 tgaacggcag gtttcgggct tagaagcaga aagtcccatt ttatcatttt tcaggttgcg
     3361 acaaggattt gcctgcttct ttgcggatga cgccaaccgc atcccggatg acggaacgct
     3421 cgtctttttc cactttatgt gtaaagcggt agtcccggac aactccttcc cagtcgtaat
     3481 ccacacacca ctcccaatgt cggcgttctg atttcatata aatgaaattg gtcggcaaaa
     3541 aattataaat cggcaggctg acctcgtgat aggcataaca accgaaaggg ttgcgcttcc
     3601 cgaaacgtgc ctctacctcc gcccgggtcg ttttgccttt aacaaccgtt tgtgcgattc
     3661 cctcttccgt ctgatatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac
     3721 agtacaaata gtacggaacc gattcacttg gtgcttcagc accttagaga atcgttctct
     3781 ttgagctaag gcgaggcaac gctgtactgg tttttgttaa tccactataa cgcaggaact
     3841 gatgttccct gtcgccgaaa ttgctggtac acgcacacag cagcaatgcc gcccacacag
     3901 ccggtttcat acacacctcc cattaaagcc aaacattata tagccgtccc gaccgattaa
     3961 attcatattt taaaacaata ttctgtttac aaaacccaca tcgtgctata atccgcaccg
     4021 attttcagac ggcatcgtca tgccgtctga aattttttca ttccaacaac aatcagcccc
     4081 gcgattacgg ctgcctgaga aagacacaaa ccatgaaaaa agtatttatc cgcaccttcg
     4141 gctgccagat gaacgaatac gacagcgaca aaatgctcgc cgtcctcgcc gaagaacacg
     4201 gcggcatcga acaggttacc caagccgacg aagccgacat catcttgttc aacacctgct
     4261 ccgtgcgcga aaaagcgcaa gaaaaagtct tctccgattt ggggcgcgtg cgtccgctca
     4321 aagaaaaaaa ccccggcctc atcatcggcg ttgccggctg cgtcgcctcg caagaaggcg
     4381 aaaacatcat caaacgcgcg ccttatgtgg acgtggtttt cggcccgcaa acgctgcacc
     4441 gcctgccaaa aatgattgtg gacaaagaaa ccagcgggct gtcgcaagtc gatatttcct
     4501 tccccgaaat cgaaaaattc gaccacctgc cgcccgcccg cgtcgaaggc ggcgcggcat
     4561 ttgtatcgat tatggaaggc tgttccaaat actgctcctt ctgcgtcgtc ccctacacgc
     4621 gcggcgaaga attctcccgc ccgctcaacg acgtattgac agaaatcgcc aaccttgccc
     4681 agcaaggcgt gaaagaaatc aacctcttgg gacaaaacgt caacgcctat cgcggcgaaa
     4741 tggacgacgg cgaaatctgc gacttcgcca ccctgctgcg catcgtccac gaaatccccg
     4801 gcattgaacg tatgcgcttc accaccagcc acccgcgcga gtttaccgac tcgattatcg
     4861 agtgctaccg cgacctgccc aaattggttt cccacctgca cctgccgatt caaagcggtt
     4921 ccgaccgcgt attgagcgcg atgaaacgcg gctacaccgc tttggaatac aaatccatca
     4981 tccgcaaact gcgtgccatc cgtcctgatt tgtgcctgag cagcgatttc atcgtcggct
     5041 tccccggcga gaccgaacgc gagttcgagc aaaccttgaa actggtgaaa gacatcgcct
     5101 tcgacttgag cttcgtgttt atttacagtc cgcgccccgg cacgcctgcc gccaacctgc
     5161 cggacgacac gccgcacgaa gaaaaagtgc gccgcctcga agccttgaac gaagtcatcg
     5221 aagccgaaac cgcgcgcatc aaccaaacga tgatcggcac ggttcaacgc tgcctggtcg
     5281 aaggcatctc caaaaaagac cccgaccagc tccaagcccg caccgccaac aaccgcgtcg
     5341 tcaactttac cggcacgccc gacatgatta accaaatgat cgatttggaa atcaccgagg
     5401 cctacacctt ctccctgcgc ggcaaagttg tcgaagccta aacccttacg ccgaaaaaat
     5461 gccgtctgaa gcgtttcaga cggcattttg ccttgtatcg gcagacgatg gcgcggctag
     5521 gcggcttaat ttgccgcatc ccgatccgac aaccacgcgc gcacacgccg ttccaccgct
     5581 tcggcactca agcccaaatc gtctaaaagt tttttcggat cgccgtgtcc ggttacggta
     5641 tcggcaacgc ccaaaagcaa aacgggtttg cagatgccgt gtttcgccaa tacttccagc
     5701 accgcgccgc ctgcgccgcc ctgttcggcg ttttcttcaa gggtaacgat gcggtcgtgg
     5761 cttcgggcaa ggcggacaat caactcttcg tctatcggtt tgacgaagcg catatcggcg
     5821 acggtggcgt tcagtttttc ggcaaccgcc aatgcggggg cgaccatact gccgaaggca
     5881 atgaaggcgg ttttcccacc ttcgcggcgg ataatgccct tgccgatttc cacggtttcc
     5941 atgccgtctg aaaccggcgc gcccgtaccc gtgccgcgcg gatagcggac ggcggcgggc
     6001 gcatccgcct gatagcaggt cgaaagcagc aggcggcatt cgttttcatc gctcggcgcg
     6061 gcgacaatca tattcggaat gcagcgcaaa aagctcaaat cgtacagacc ggcatgggtc
     6121 gggccgtccg cgccgacgat gcccgcgcgg tcgacggcaa acaaaacggg caggttttgc
     6181 agggcgatgt cgtgcaccag ttggtcgtag gcgcgttgta aaaaggtgga ataaatcgcc
     6241 acgacgggct tcatgccttc gcacgccaaa ccgccggcaa aggtaacggc gtgctgctcg
     6301 gcaatgccga catcgaaata acggtcgggg aatcgttgtt caaactccac cagtccgctg
     6361 ccctcgcgca tcgcgggcgt gatggcgatc aaacgctcgt ctgccgccgc ccggtcgcac
     6421 agccatttgc cgaacacttg ggtataggtc ggtttggcgg cgggcttggg ttctttttca
     6481 gacggcattt gcgccgcgct ttctttaggc aggttggcga cggcgtggta tttgacggga
     6541 tcgttttcgg cgagtttgta gccgttgccc tttttggtga tgacgtgcag cagttgaggg
     6601 cctttgcggc tgcgcaagtc tttcaatacg tccaccagat tttcgacgtt gtgtccgtcc
     6661 acggggccgg tgtagcggaa gccgaagttt tcaaacaaag acagcgactg tttggcgtgt
     6721 tcggcttctt cggcaagggt tttgattttg tgttcgactt tttgggcgag ctccatcgcg
     6781 ccgggtattt tgtctaatac cttgcccgtt tgcgctttga cggtactcaa caggccgtgc
     6841 atatcgcgca cgacgttgct ggcgaggtat ttcggcagcg cgccgacgtt gggggaaatc
     6901 gacatttcgt tgtcgttgag gacgaccagc aaatccacat ccatatcgcc tgcgcaattc
     6961 aaggcttcaa acgcctgccc cgccgtcatc gcgccgtcgc cgatgatggc gacgctgcgg
     7021 cggtcgctgc ccaagagttt gtctgccgcc gccatgccca acgccgcgcc gatggatgtg
     7081 gaagaatgcc ctacgccgaa cgcgtcgtat tccgactcgc tgcgttttgg gaagccagct
     7141 aaaccgccgt attggcgcat ggtgtgcatc tggtttttcc tgcctgtcag gattttgtgc
     7201 ggatagcttt ggtgtccgac atcccacacc agcttgtctt cgggcgtgtc gtacacatag
     7261 tgcagggcga tggtcagttc gaccgcgccc aaattgctgg cgaaatgccc gccggtctgc
     7321 ccgacagatt ccagcagaaa ggtgcgcaac tcgccggcaa ggcgcggcag ctgttttttg
     7381 tccagacggc gcaaatcttg cgggctgtca atcaggtcga gtagggggct tgggttcatg
     7441 gtgtgtcttt tttatgtgtc gtccaggtgc aacgaccaat tatatatcaa gagcgtgcgg
     7501 ctgacggctg attttgccgt atgtcattcg tcctgccgct tggcgcgcgg gtgggcttcg
     7561 tcatacaggc gggcgatgtg gtcgaaatcg agtttggtgt aaatctgcgt ggtcgaaagg
     7621 ctgctgtgcc cgagcagctc ctgcaccgcc cggatgtcgc gcgaagcctg caacaggtgt
     7681 ccggcgtagc tgtggcgcat catatgcggc gaaacgtgcc tgccgtcgcc gttttgcgcc
     7741 gcccattgcg ccaaacgttt ttggatttgg cgttggctca ggcgcgtgcc gttcctgccg
     7801 gtaaacaggg ctttgccgtc cgatgccgtc tgacgcagcg gcagatagtt tttcagggct
     7861 tccacgcttt tgccgaccag cggcacctgc cgctgcttgc gccctttgcc ggtaacgtgt
     7921 acccacgcct cgtccaaata gacatcatct gcattcaagc cgtgtatctc gctcacgcgc
     7981 aaaccgctgc cgtacatcag ctcgaacagg gcgtgatcgc gcaccgccag cgggtcgccg
     8041 ccgtccacgg gcaaatccag catccggttc agccattcct gcgacagggc tttgggtacg
     8101 cgctcgggct gcttcggcgg tttgatgtcg gcggtcgggt cggtatgcag cagaccgcgt
     8161 tccaccagcc aaacacaata ctgccgccaa gacgacagct tgcgcgccag cgtccgcgca
     8221 ttcaaaccgc gctgcgacag ccgccgcaac gccgccgtaa aatcgcgccg cgacaagtcc
     8281 tgcggcacgc cgccttcatc ttcagacggc atttgtgcca acagcgcaaa cagttcttcc
     8341 aaatcgcgcc ggtatgccgc caccgtgtgc tccgacttgc cctcgcgcac gatgttttcc
     8401 aaataagcgt caaaatgcgc cgcaaacccg tccaaaacca taaccgcccc acacaaatat
     8461 caaaaaaaca gtgaaaacat tatcataaac gacacaccaa aaccccgaac cgcccaaaat
     8521 ccgacccgtc cgcccgccgt tatcgttaac ataaaatcaa tgagccgttt atggtttttt
     8581 gccgcaaaaa acattataat ccgccttatt tacctattgc ccaaggagac acaaatggca
     8641 ctcgtatcca tgcgccaact gcttgatcat gctgccgaaa acagctacgg cctgcccgcg
     8701 ttcaacgtca acaacctcga acaaatgcgc gccattatgg aagccgccga ccaagtcaac
     8761 gcgcccgtca tcgtacaggc gagtgcaggt gcgcgcaaat atgcgggcgc gccgtttttg
     8821 cgccacctga ttctggcggc tgtcgaagaa tttccgcaca tccccgtcgt gatgcaccaa
     8881 gaccacggcg catcgcccga cgtgtgccaa cgctccatcc aactgggctt ttcctccgtg
     8941 atgatggacg gctcgctgat ggaagacggc aaaacccctt cttcttatga atacaacgtc
     9001 aacgccaccc gtaccgtggt taatttctcc cacgcttgcg gcgtatccgt cgaaggcgaa
     9061 atcggcgtgt tgggcaacct cgaaaccggc gaagccggcg aagaagacgg tgtaggcgca
     9121 gtgggcaaac tttcccacga ccaaatgctc accagcgtcg aagatgccgt gcgtttcgtt
     9181 aaagataccg gcgttgacgc attggcgatt gccgtcggca ccagccacgg cgcgtacaaa
     9241 ttcacccgtc cgcccacagg cgacgtgttg cgtatcgacc gcatcaaaga aatccaccaa
     9301 gccctgccca atacacacat cgtgatgcac ggctccagct ccgttccgca agaatggctg
     9361 aaagtcatca acgaatacgg cggcaatatc ggcgaaacct acggcgtgcc ggttgaagaa
     9421 atcgtcgaag gcatcaaaca cggcgtgcgc aaagtcaaca tcgacaccga cttgcgcctt
     9481 gcttccaccg gcgcggtacg ccgctacctt gccgaaaacc cgtccgactt cgatccgcgc
     9541 aaatacctga gcaaaaccat tgaagcgatg aagcaaatct gcctcgaccg ctatcttgcg
     9601 ttcggttgcg aaggtcaggc aggcaaaatc aaaccggttt ccttggaaaa aatggcaagc
     9661 cgttatgcca agggcgaatt gaaccaaatc gtcaaataac aggttgcttg taaacaaaat
     9721 gccgtctgaa ccgttgttcg gacggcattt gatttttgct tctttgacct gcctcattga
     9781 tgcagtatgc aaaaaaagat accataacca aaatgtttat atattatcta ttctgcgtat
     9841 gactaggagc aaacctgtga accgaactgc cttctgctgc ctttctttga ccgccgccct
     9901 gattctgacc gcctgcagca gcggaggcgg cggtgtcgcc gccgacatcg gcgcggggct
     9961 tgccgatgca ctaaccacac cgctcgacca taaagacaaa agtttgcagt ctttgacgct
    10021 ggatcagtcc gtcaggaaaa acgagaaact gaagctggcg gcacaaggtg cggaaaaaac
    10081 ttatggaaac ggcgacagcc tcaatacggg caaattgaag aacgacaagg tcagccgctt
    10141 cgactttatc cgtcaaatcg aagtggacgg acaaaccatc acgctggcaa gcggcgaatt
    10201 tcaaatatac aaacagaacc actccgccgt cgttgcccta cagattgaaa aaatcaacaa
    10261 ccccgacaaa atcgacagcc tgataaacca acgctccttc cttgtcagcg gtttgggcgg
    10321 agaacatacc gccttcaacc aactgcctga cggcaaagcc gagtatcacg gcaaagcatt
    10381 cagctccgac gacccgaacg gcaggctgca ctactccatt gattttacca aaaaacaggg
    10441 ttacggcaga atcgaacacc tgaaaacgcc cgagcagaat gtcgagcttg cctccgccga
    10501 actcaaagca gatgaaaaat cacacgccgt cattttgggc gacacgcgct acggcggcga
    10561 agaaaaaggc acttaccacc tcgccctttt cggcgaccgc gcccaagaaa tcgccggctc
    10621 ggcaaccgtg aagataaggg aaaaggttca cgaaatcggc atcgccggca aacagtagca
    10681 tttgaaaaat gccgtccgaa cacgatagtt taccgttcgg acggcatttt tgcgccatac
    10741 tgccctagga cacgacggca cggcaggcaa gccggtttca ctatataatg ccgtctgaac
    10801 caacgagaac cacaccatgc aagccgattt caaccgcccc gtcctcgccg tcgataccgg
    10861 aacttcctat ttgtcgctcg cgctgcgcgc cgacggcgaa accctcctgt tccatcagga
    10921 ggccggcagc cgccagtccg aactgatcct gccggaaatc cgcaccctat tccgaaatgc
    10981 gggcattacc gccgccgatt tgggggcgat tgtttatgcg aaaggtcccg gcgcgtttac
    11041 cggactgcgt atcggcatcg gcgtggcgca aggtttggca acgccattca atacgccttt
    11101 aatcggcatc cccacgctcg atgccgccgc ctcgctgccg ccgccgcaaa gctgcatact
    11161 cgccgctgcg gacgcgcgta tgggcgaagt gttttatgcg tggttcgaca cgctgaaccg
    11221 ccgccgtttg agcgattatc aggtcgggcg ggcggcagac atccggctgc cggagggatg
    11281 tgccttttca gacggcatag gcagcgcgtt cgcgttggaa gaaacgccgc cgttttcagg
    11341 caaacccgat atgccgaccg ccgccgactt cctcaatctg gcggcggaag gcggttttcc
    11401 cgccgtcgct gctgcacact ccgaactgct ctacgtccgc aacaaaatcg ccctaaccgc
    11461 caaagaacag gcggaacgga aagcccgaac atgaacatcc gccgcgcctc accgtccgac
    11521 tgcgccgcac tcgccgcgct cgatgccgtc tgcaacccgt ccgcgtggac gcaacgccaa
    11581 tttgagtccg cattggtttc gccgtccgaa caggttttcc tcgcggaaaa agacggcggg
    11641 attgccgcct ttatcgtttg gcagaacctg cccgacgaat ccgaactgca cctgattgcc
    11701 accgcgcccg aatgccgccg ccgtggcgtt gcgtccgccc tgctcgaata ttggttcgca
    11761 catctgcccg aagacacgca acgcctgctg ctcgaagtcc gtgcaggcaa caccgccgca
    11821 caggcactgt acgcggcgca cggctttacc gatgcgggca ggcggaaaaa ctattaccgt
    11881 acagccgacg gtaaaaccga agatgctgtc ttaatggaga aaatatgtta agcgcgcgct
    11941 acctccacct gcacgaagct ttgggtttag gcccgatgtg gctgaaacag gccgccgccg
    12001 tcctgccgcc caaaaacaca cccgcaaccc cggcacaggc acgtcctcaa accgtccgcg
    12061 ccgccccgat ccgcccttcc caaccccata acggtcaggc gcggctcgaa acgatgaaag
    12121 cgttggaaac cgccgccgta catacgcgca aacccgcgcc tgaaaccgaa acgcccccgc
    12181 ccggcctttc agacggcatc gcccccgttc ccgccgcttc gggcataacc aaacttgccg
    12241 tcgtcagcct ttgcccaccg attgaggatg cggtttacgg gcaactgttc tacggcaaag
    12301 cgggtgttct gcttgacaac atactcaaag ccgtaggact ggatgccgcc tatgtccaca
    12361 aaacctgttg ggtgaaaacc gccgccgtcg gcaacccgat gccgtctgaa gcggcaatcg
    12421 aaaccgcgac ggtttccgtc atgcaggaac tcgacggctg ccgcgccccc gccgtcctat
    12481 tcctcggaca ggcttttgtc cagcctgaac ggcaaacgat gattgaaact ttgtgcggca
    12541 gccgtccctt cttcatcatc gaccatcccg cccggctgtt gcgccaaccc gaacttaaag
    12601 cccgcgcctg gcaggtgttg aaacagttga aacgcgcctt gcggcaaggc ggcggcagtt
    12661 gaagcgcacc gcacggggcg gtagaatcgc aactgcgtcc caatatctga cagaaagcac
    12721 aaaatgaccg atttccgcca agatttcctc aaattctccc tcgcccaaaa tgttttgaaa
    12781 ttcggcgaat ttaccaccaa agccggacgg cggtcgccct atttcttcaa tgccggcctc
    12841 tttaacgacg gcttgtccac gctgcaactg gcaaaatttt acgcacaatc catcattgaa
    12901 agcggcatcc gattcgatat gctgttcggc cccgcctaca aaggcattat tttggcggcg
    12961 gcaaccgcga tgatgctggc ggaaaaaggc gtgaacgtcc cgtttgccta caaccgcaaa
    13021 gaagccaaag accacggcga aggcggcgtg ttggtcggtg cgccgcttaa aggccgcgtg
    13081 ctgattatcg acgacgtgat ttccgccggc acatccgtac gcgaatcgat caagctgatt
    13141 gaagcggagg gcgcaacccc cgccggtgtc gccatcgcgc tcgatcgcat ggaaaaaggc
    13201 acgggtgagt tgagcgcggt tcaggaagtg gaaaaacaat acggtctgcc cgtcgccccc
    13261 atcgccagcc tgaacgattt gtttattctg ttgcaaaaca accccgaatt cggacagttc
    13321 ctcgaacccg tccgagccta ccgtcggcag tacggcgtag aataaaaaca aagcatatgc
    13381 cgtccgaacc gccttacgcc tcagacggca tcaaacctga cacacacgag gaaataccat
    13441 gcccgcctgt ttctgccccc actgcaaaac ccgtctctgg gtcaaagaaa cccaactcaa
    13501 tgtcgcccaa ggcttcgtcg tctgccaaaa atgcgaagga ctgtttaaag ccaaagacca
    13561 tctggcaagc acgaaagaac ccatattcaa cgatttgccc gaggctgttt cggatgtcaa
    13621 actcgttcac cgtatcggca cgcgcgccat cggcaagaaa cagatttccc gtgacgaaat
    13681 cgccggcatc ctcaacggcg gcacaaccca gcccgatatt ccgcccgcaa ccgccgccac
    13741 ccctgctgcc gcaccgcagg ttaccgtacc gcccgccgcg cccgcccgtc aggatgggtt
    13801 caactggacg attgcaaccc tgtttgccct tatcgtcctc attatgcagc tttcctacct
    13861 cgtcatccta tgatcgtgcc cgacctcttt gtcgcccact tccgcgaagc cgccccctac
    13921 atccgccaaa tgcgcggcaa aacgctggtc gccggcatag acgaccgcct gctcgaaggt
    13981 gataccttaa acaagctcgc cgccgacatc gggcttttgt cgcaactggg catcaggctc
    14041 gtcctcatcc acggcgcgcg ccacttcctc gaccgccacg ccgccgcgca aggccgcacg
    14101 ccgcattatt gccggggctt gcgcgttacc gacgaaacct cgctcgaaca ggcgcagcag
    14161 tttgccggca ccgtccgcag ccgttttgaa gccgcattgt gcggcagcgt ttccgggttc
    14221 gcgcgcgcgc cttccgtccc gctcgtatcg ggcaacttcc tgaccgcccg tccgataggt
    14281 gtgattgacg gaaccgatat ggaatacgcg ggcgttatcc gcaaaaccga caccgccgcc
    14341 ctccgtttcc aactcgacgc gggcaatatc gtctggctgc cgccgctcgg acattcctac
    14401 agcggcaaga ccttccatct cgatatgctt caaaccgccg cctccgtcgc cgtctcgctt
    14461 caggccgaaa aactcgttta cctgaccctt tcagacggca tttcccgccc cgacggcacg
    14521 ctcgccgtaa ccctctcggc acaggaagcg caatcgctgg cggaacacgc cggcggcgaa
    14581 acgcgacggc tgatttcgtc cgccgttgcc gcgctcgaag gcggcgtgca tcgcgtccaa
    14641 atcctcaacg gtgccgccga cggcagcctg ctgcaagaac tcttcactcg caacggcatc
    14701 ggcacgtcca ttgccaaaga agccttcgtc tccatccggc aggcgcacag cggcgacatc
    14761 ccgcacattg ccgccctcat ccgcccgctg gaagaacaag gcatcctgct gcaccgcagc
    14821 cgcgaatacc tcgaaaacca catttccgaa ttttccatcc tcgaacacga cggcaacctg
    14881 tacggttgcg ccaccctaaa aacctttacc gaagccgatt gcggcgaaat cgcctgcctt
    14941 gccgtctcgc cgcaggcaca ggacggcggc tacggcgaac gcctgcttgc ccacattatc
    15001 gataaggcgc gcggcatagg cataagcagg ctgttcgcac tgtccacaaa taccggcgaa
    15061 tggtttgccg aacgcggctt tcagacggca tcggaagacg agttgcccga aacgcggcgc
    15121 aaagactacc gcagcaacgg acggaactcg catattctgg tacgtcgcct gcaccgctga
    15181 ccgcaacgga aagccgccgc agaaatgccg tctgaacccc gtttcagacg gcatttcccc
    15241 gattataaag acctgcccaa ccctcaagga ccccgatgaa atcctacccc gacccctacc
    15301 gccattttga agacctcgat tccgccgaaa cgcaaaactt cgctgctgaa gcgaatgccg
    15361 aaacgcgcgc gcgtttttta aacaacgaca aggcgcgcgc attgtctgac ggtattttgg
    15421 cgcagttgca ggacacgcgg cagattccgt tttgtcagga acaccgcgcg cggatgtacc
    15481 atttccatca ggacgcggaa tatccgaagg gcgtgtaccg cgtgtgtacc gcggcgacat
    15541 accgttccgg ctatcctgag tggaaaatcc tgttttcagt ggcggatttc gacgaattgc
    15601 tcggcgacga tgtatatttg ggcggcgtgt cgcacttggt ggaacagccc aaccgtgcgt
    15661 tgttaacact gagcaaatcg ggcggcgata cggcgtacac gctggaagtg gatctggaag
    15721 caggggagtt ggtagaaggc ggttttcact ttccggcagg caaaaaccat gtgtcgtggc
    15781 gcgatgaaaa cagcgtgtgg gtgtgtccgg cttggggcga acgccagttg accgaatcgg
    15841 gctatccgcg cgaggtgtgg ctggtggaac gcggcaagag tttcgaggaa agcctgcctg
    15901 tgtatcaaat cgataaaggt gcaatgatgg tgaacgcgtg gcgttatctc gatccgcaag
    15961 gttcgccgat tgatttgatt gaagcgtcgg acggttttta caccaaaacc tatttgcagg
    16021 tgtcgtcgga aggcgaggcg aaaccgttaa acctgcccaa cgattgcgac gtggccggct
    16081 atctagccgg acatcttttg ctgacattgc gtaaagactg gcaccgcgcg aaccaaagct
    16141 atccgagcgg cgcattggtg gcagtaaaat taaaccgagg cgaactcggg gcggcgcagc
    16201 ttttgtttgc gcccgatgaa acgcaggcat tggaaagcgt ggaaacgacc aagcgttttg
    16261 tggtagcgag cctgctggaa aacgtacaag gccgtctgaa agcgtggcgg tttgccgacg
    16321 gcaaatggca ggaagtcgaa ctaccgcgcc tgccttcggg cgcgttggaa atgaccgacc
    16381 aaccgtgggg tggcgacgtg gtttaccttg ccgccggcga tttcaccacg ccgctggcgc
    16441 tgtttgcatt ggatttgaac gtgatggaac tgaccgtcat gcgccgtcag ccgcagcagt
    16501 tcgattcaga cggcatcaac gtgcggcagt tttgggcggt ttcgtccgac ggcgagcgca
    16561 ttccttattt ccatgtcggc aaaaacgccg cgcccgacac gccgactttg gtctatgctt
    16621 acggcggttt cggcgtgccc gagctgccgc attatctggg cagcg
//
LOCUS       DAWHXZ010000020        16599 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000020 DAWHXZ010000000
VERSION     DAWHXZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16599
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.020"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(21..1079)
                     /gene="dinB"
                     /locus_tag="WIE35_000432"
     CDS             complement(21..1079)
                     /gene="dinB"
                     /locus_tag="WIE35_000432"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ4153398.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYDSEVAKEIRAAIFAETGLTASAGIASNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMQTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCASLLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
     gene            complement(1223..2416)
                     /locus_tag="WIE35_000433"
     CDS             complement(1223..2416)
                     /locus_tag="WIE35_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ4153399.1"
                     /translation="MYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPDGSPVFTGFSG
                     TVPVWKTVKVADDHVPALYNYAKYLNTNKTHSLTASTRFNVTGRLHLLGGLHYTRYET
                     SQTKDMPVRYGQPASDFQTASSIKADQDHYTAKMQGHKLTPYAGITYDLTPQQSIYGS
                     YTKIFKQQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVD
                     FGYVPGAGGKQGSFQTVAKPIGKVVSRGAEAELSGELNEDWKVFAGYTYNKSRYKNAA
                     EVNAERLAKNTGADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNI
                     RQGGYGLIDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMK
                     LDWQF"
     gene            complement(2452..4311)
                     /locus_tag="WIE35_000434"
     CDS             complement(2452..4311)
                     /locus_tag="WIE35_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ4153400.1"
                     /translation="MNQNHFSLKILTVMLLSAYGGSFADGVVPVSDGNTVSLDTVNVR
                     GSHALSGKTEKTRSYTIDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMK
                     NTTGVNVVRDSGLQTRFLSRGFYIDQIGEDGITVNVAGRSGYTAKIDVSPSTDLAVYD
                     HIEVVRGATGLTQSNSEPGGTVNLIRKRPTASFKHTGELTADHRGSRRAVLDVSGSLN
                     KANTLRGRLVGAEEYKKSFKDRVWGRKHMVYGIAEADAGDSSVLTLGGMYQKSREVPD
                     FSGIILSCENQKTAPFSSTPACNRPLQLPRNTYLGEDWSRLSADKYNLFSGFKHVFDN
                     GWQLNAEVSYTKNESDAKVGQFFLKNEHAAGLSDEDAVGFLTEKNEVIPFEPKDKALE
                     KLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYRSRRAAERKAGFDECMSAPFALDFI
                     CQGSWGDPGVDADKSEFVDKALAKEGIFNNAAQRFPNSLYDSSFNRKATANRRYSYMP
                     LRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDEKIRSEYLEIYERRHRVRPNT
                     GATHGVYAGSCQGSRTVICLLLWSGGIKNPIGRRTMKKATVPFMPKNAGMPRK"
     gene            4585..5364
                     /locus_tag="WIE35_000435"
     CDS             4585..5364
                     /locus_tag="WIE35_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ4153401.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSIRFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYVMHRNRIPGQIMMENGF"
     gene            complement(5574..6986)
                     /locus_tag="WIE35_000436"
     CDS             complement(5574..6986)
                     /locus_tag="WIE35_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ4153402.1"
                     /translation="MLFEFKENKMIVTSRWPLLEKAFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGRLHAHKVKAELVRHYGVDCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFSNKKAARRALSSWAETYGLCPASAGILPDGYAEDEPCPVYVSGRCDKACD
                     RSDEQVLAFAHKLPVLDWGKMHEVEITETDPLTGKTVVLHGMGGALEMDDGLWYFDKD
                     LPDVFKAKFKTDRKNIKEIG"
     gene            7077..7153
                     /locus_tag="WIE35_000437"
     tRNA            7077..7153
                     /locus_tag="WIE35_000437"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7111..7113,aa:Arg,seq:acg)
     gene            7171..7245
                     /locus_tag="WIE35_000438"
     tRNA            7171..7245
                     /locus_tag="WIE35_000438"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7204..7206,aa:Glu,seq:ttc)
     gene            7805..8980
                     /locus_tag="WIE35_000439"
     CDS             7805..8980
                     /locus_tag="WIE35_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ4153403.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(9322..9870)
                     /locus_tag="WIE35_000440"
     CDS             complement(9322..9870)
                     /locus_tag="WIE35_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ4153404.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(9873..11369)
                     /gene="ccoG"
                     /locus_tag="WIE35_000441"
     CDS             complement(9873..11369)
                     /gene="ccoG"
                     /locus_tag="WIE35_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ4153405.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRVSVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            11897..13876
                     /gene="tkt"
                     /locus_tag="WIE35_000442"
     CDS             11897..13876
                     /gene="tkt"
                     /locus_tag="WIE35_000442"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ4153406.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGIKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            14017..15405
                     /gene="fumC"
                     /locus_tag="WIE35_000443"
     CDS             14017..15405
                     /gene="fumC"
                     /locus_tag="WIE35_000443"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ4153407.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(15470..16393)
                     /locus_tag="WIE35_000444"
     CDS             complement(15470..16393)
                     /locus_tag="WIE35_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ4153408.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRLFKRR"
BASE COUNT         4146 a         4718 c         3897 g         3838 t
ORIGIN      
        1 gacggcgcgc gtaaagcggt ttacgcccac aaatcctgct gctggttttt cggcacaaga
       61 tgccccacgc cgataccgat aaggcggaac gcgtcttccg tctgcggcgg gacgcgcgcc
      121 atcaacattt gcgccgcctg aagcagggag gcgcagtcgg gcaatacgga ggaataagtc
      181 agcgtgcgcg tgatgatgcg gaaatcgtag gtcttcagct tgagcgttac actttgggct
      241 tcgacgtttt tgcgcgtgat ttgccgccac aagtcttcgg caagatgggg caagtgtccg
      301 gcagcctgct cgagcggcag gtcttcgggc agggtgattt ctgtggagat ttggaggcgt
      361 tcgcgttcgg ctttgacggg gcgttcgtcc gtaccgcgcg ccaaatcata gaggcggtag
      421 ccgtagcgtc cgaaatggtt taagagctcg ccgcgctcga aacggcgcaa atcacccgcc
      481 gtctgcatac ccagcgactg catttttttc agcgttacct tgcccacgcc ggggattttg
      541 cccaaaggca gggtttccaa aaatgccatg actttgtgcg gcggcaacac aaactgcccg
      601 ttcggcttgc gccagtccga cgcgattttc gccagaaatt tgttcgacgc gatgcccgcg
      661 gatgcggtca aacccgtttc cgcaaaaatg gcggcacgga tttctttggc aacttcactg
      721 tcgtaaggga tgtttttgaa attacgggta acgtcaagat aggcttcgtc cagcgacaag
      781 ggttcgatta aatcggtata acgcctgaat acggcgtgaa tctgcgcgga aacctgacgg
      841 tacaaatcga aatgcggcgg cacatacacc gcttgcggac acagcctttt cgccgttgcc
      901 accgacatcg cggaatgcag cccgaactgc cgtgcctcat acgatgcggc gcaaatcacc
      961 gaacgcgcgc cctcccacgc gacgaccacc ggccgccctt tcaaatgcgg ctgttcgcgc
     1021 agctctaccg atgcgtagaa tgcgtccatg tcgatgtgga taattttgcg tgaagacatc
     1081 ggctcttctg aggataaaag ggatattcta ctgccggcat cgggcaaatt ccaaatatac
     1141 gccccgatag gcctgcctcc ataaaaatgc cgtctgaaac ataccctgtt tcagacggca
     1201 tccgcaaaac tacggttttc aattaaaact gccaatccag tttcatgctg acagtgcgcg
     1261 gttctccata gaagttgttt gcgccgcgcg tacggttgta gttgttctca aaataagtgc
     1321 gtccgtttaa gttcgtaccg atgaggctca atttagcgtg tttgcccaat tcgtaacgga
     1381 cgaaaccgtc tatcagcccg tagccgccct gcctgatgtt gtacaggctg cttgtgccgc
     1441 tttgtgcgga cacgccgccg ccgacggtca gccccgtatt cggtatatgg aagctcgttc
     1501 cgaaacggaa tatgtgcacg ggtgtgaaat tgctgaagtt gtacgggtct gcgcctgtgt
     1561 ttttggcgag gcgttcggcg ttgacttcgg cggcgttttt gtagcggctc ttgttgtagg
     1621 tgtaacccgc aaagactttc caatcttcgt tcaactcacc cgacaactcg gcctccgccc
     1681 cacggctgac cactttgcct atcggtttgg caacggtttg gaacgacccc tgcttgccgc
     1741 ctgctccggg aacatagcca aaatcgacga ccgtgcggtt tttctgttcg aggtaaaaca
     1801 atgcgaacga agcattcagc cgtccttgca agaatgcgcc tttccagcct acctcatagt
     1861 ttgtgccgac caaaggcggc aaaacggttt tggcactgac atcgacatta tcctgctgtt
     1921 tgaagatttt ggtataactt ccgtaaatac tctgttgcgg tgtcaagtca taggtgatgc
     1981 ctgcataggg cgtcaattta tgaccttgca tcttggccgt gtaatggtcc tgatccgcct
     2041 taatactcga tgctgtctga aaatcgcttg ccggctgccc atagcggaca ggcatatctt
     2101 tggtttgcga ggtttcatag cgcgtgtagt gcagcccgcc caaaaggtgc agtcggccgg
     2161 ttacgttgaa acgcgtgctg gcagtcagcg aatgggtttt gttggtgttg aggtatttgg
     2221 cgtagttata cagcgcagga acatggtcat cggccacttt aacagttttc caaaccggca
     2281 ccgtaccgga aaaaccggta aagacaggcg agccgtcggg attggtctcc tgaatcttgt
     2341 tgcctttttc gtccagctca tatacatcga catataccgg tgtccggctg ccgctgtatt
     2401 cgtcatagta atacacctgc ttgccttcgg catcgagctt gggctcggtt tttattttct
     2461 tggcattcct gcattcttcg gcataaacgg tacggttgcc tttttcatcg tacgcctgcc
     2521 aatcgggttc tttatgcccc ctgaccaaag gagaagacaa atcaccgtcc ggctcccctg
     2581 acaacttccc gcatacacgc cgtgcgttgc ccctgtattc ggacgtactc tgtggcggcg
     2641 ttcgtagatt tccagatatt cggaacgtat cttttcatcg ccgtaggcat agccgacaaa
     2701 gaaatcatgc tcccgcccga acagcccata tgtgccggtc aggtcaagtt taattcccca
     2761 ttggcggtcg tctttggtat gccgcaacgg catataactg tatcgtcggt tggcggtagc
     2821 cttccgatta aaggaagagt catacaggct gtttggaaaa cgttgtgccg cattattaaa
     2881 gatgccttcc ttcgcaaggg ctttatcgac aaattccgac ttgtcggcat caacacccgg
     2941 atccccccaa gaaccttgac agataaagtc cagcgcaaaa ggggcactca tacactcgtc
     3001 aaaaccggct ttgcgttctg cggcacggcg gctgcggtac tgctcgaaag cagtattatc
     3061 gaaacggttt ttaacaaaat cgtctttgcg ctcacggtat tccttggcgg tttcgtcacg
     3121 atatgctttc agtttctcca atgccttatc tttcggctcg aacgggatga cttcgttttt
     3181 ttcggtcaaa aagcctaccg catcctcatc tgacaaaccc gccgcatgtt cgtttttcag
     3241 aaaaaactgc cccaccttcg catcggattc attcttggta taagacactt cggcattgag
     3301 ctgccaaccg ttgtcaaaca catgtttgaa acctgagaaa aggttgtatt tgtcagcact
     3361 caaccgcgac caatcctccc ccaaataagt gttgcgcggc agttgcaaag gccggttgca
     3421 ggcaggcgtt gaactgaacg gggcagtttt ctgattttca caggacaaaa taatgcccga
     3481 aaaatcagga acctccctac tcttctgata catgccgccc aaagtaagca cactgctgtc
     3541 gcccgcatcg gcttcggcaa tgccgtaaac catatgtttc ctgccccaaa cgcggtcttt
     3601 aaacgatttt ttatactctt ccgcacccac caaccttccg cgtaaggtat tcgccttatt
     3661 caggctgcct gaaacatcca acactgcacg ccggctgccg cgatggtcgg cggtcagctc
     3721 tccggtatgt ttgaaagaag cggtaggtcg cttacggatc aaattgacgg ttccacccgg
     3781 ctctgaattg gattgggtca accccgttgc accccgtaca acttcaatat ggtcataaac
     3841 cgccaaatcg gtactcggag acacgtcgat tttcgccgta tatcccgaac ggcctgcaac
     3901 attgacggta ataccgtctt caccaatctg atcaatatag aaaccgcgtg acaaaaaccg
     3961 cgtctgcaag cctgaatcgc gcacaacgtt gacacccgtc gtgtttttca ttgcctcttc
     4021 aagcgtatgc accgccttat cgtcaaggcg gctgcgcgtg atgacgctga ccgactgcgg
     4081 cgtatccttg cccgcaatcc tcatacctgt ggcggtggac atccgatcta tcgtataaga
     4141 acgggtcttc tcggtcttgc ccgacagagc atgagagccg cgtacattga ccgtatccag
     4201 actgacggta ttgccgtctg aaacaggcac aacaccgtct gcaaaagaac caccgtaagc
     4261 cgataacagc ataacggtca gaattttaag tgaaaaatga ttttgattca tagagacctc
     4321 tgtaatatgc aagtgtgcaa atcgtccaaa ggccctcaca actgttttga ttttttatat
     4381 taattgaaaa aaagtaattc tcaattaaat ttatagatgg gattgtattc ccattttgac
     4441 aaaaaacaaa ctgctcctta ccgtttattt caaaaaacga taatattgta ttgaaaaata
     4501 tccgaattta aatacagatc gtcaatgcgg aaaaaaacac ccaaattggc tataatcccg
     4561 acaaacacac tcaaggacaa caacatggca gcctcgcccg aagcaaaatt cactgaagaa
     4621 aagattttgt gggtcaaaca ccacacgccg aaactcatca ctttcgccat cagccgtcca
     4681 gaatcctacc gttttaaagc cggacagttc tcccgactcg gtttctacga aggggaaggc
     4741 tttatttggc gtgcctattc catcgtttcc gcagaatatg ccgacacgct cgaatatttt
     4801 gccgtactca tccaagacgg ccccatgtcg atccgtttcg ccaaaatgca acagggcgac
     4861 accatcctgc tcgataaaaa tgccaccggc ttcctcctgc ccgaacgctt ccccgacggc
     4921 aaggatttgg tgatgctctg caccggctcc ggcatcgccc ccttcctttc cattctcgaa
     4981 caacccgaaa tccggcagcg tttcgatacc gtcaacctga tacattccgt atcttttccc
     5041 gaagaattga ttttcaacga ccggctcgcc gcattgtccg aacaccccct ggtaggcgaa
     5101 tacggacact cgttccgttt cgtccctgtt accacccgtg ccgccaaccc ctcgggctta
     5161 agcggaaaac gcattccgga actcttaaaa aacagcagca tcgaacaggc gctgcatacc
     5221 aagttcaccc cggaatccac acggtttatg atttgcggca acccggaaat ggtcaaagac
     5281 actttccaaa cgctgctcga catgggttac gtcatgcacc gcaaccgcat tcccggtcaa
     5341 atcatgatgg aaaacggctt ctaaaaacca ccctgcttgt ccgatgcctt cggatggaag
     5401 ggcaaaccga cacggcacga aaaccgcgtc ggtaaaaatg ccgtctgaaa aaattcagac
     5461 ggcatcttcg gacacattac ctgaaacggt aacacattcc aacccaaacc gatcaggcaa
     5521 tcaatacgaa cgactgttta catacttacc gactttcacc aaccggtatt gatttaaccg
     5581 atttccttaa tatttttcct gtccgtttta aacttcgcct taaacacatc cggtaaatct
     5641 ttatcgaaat accaaagccc gtcatccatt tccaatgcgc cgcccattcc gtgcagaacg
     5701 actgttttcc ccgtcagcgg atcggtttcg gtaatttcca cttcgtgcat tttgccccaa
     5761 tccaaaacag gcaacttgtg tgcaaacgcc aaaacctgtt cgtcagagcg gtcgcacgcc
     5821 ttatcgcatc ggcctgaaac atatacggga caaggctcat cctctgcata accgtccgga
     5881 aggataccgg ctgaggcagg acacaatccg tatgtttccg cccacgacga caaagcccgt
     5941 ctcgccgcct ttttattgga aaataatccg gtaggcggat tatccgtcac accgtttttc
     6001 aaagccgctg ttttcgcatt caacatgccg tctgaacctt tttcaaacct gacggtcgta
     6061 aatgttttaa gcagattttt ggcagatacg taacaatcca ccccataatg cctgaccaat
     6121 tccgctttaa ccttatgggc atgcagcctg cccaatgcgg gaaaaaaacg gacttcctcc
     6181 gtattgcacc aatcaaacgg ggcttttccg gagtccaata aagccgaaac ctcgctatat
     6241 acccgttcaa acgtaccgat gtaatttact ttccctccgc cgtcgaaaca ggccagcacc
     6301 cccataccgt caggcaaacc gtacaactgt tccctcaacc gttcgggcag cgcggcaggc
     6361 aacggtttcg gattcatcaa acggaaacac tgcctgatcc atgcctcgac cccgtgttcc
     6421 gacagactgt attccaaata atcgcacaat gccgatacat ccgccatcgc acgatgcctg
     6481 tcttccacaa caatccccaa cctttcgatg atactgtcca ggctgtgctt gtaaaattgc
     6541 ggatacagac accgggacag ttgcacactg cacaaagcag gcgatgaaaa tccgataccc
     6601 gcacgatgaa actcatgctt taaaaacgta tagtcgaaac ggctgttatg tgcaaccagc
     6661 acacaaccct tcaataccga aaacaactcg ccggcaatct ctgcaaaaac aggtgcgtcg
     6721 gcaaccatgc cgtctgaaat ccccgtcagc cccgccacaa actgcggaat cggtttttga
     6781 ggattaacca accactcatg cctcaccacc cttccctgct caaacttgac caaagccact
     6841 tcggttaccc tgtcttcata cagattgccg cccgtcgatt ccaaatcaac cacggcaaca
     6901 ggcattccaa accgtaaaaa tgccttttcc agcaagggcc aacgggaagt aacaatcatt
     6961 ttattctctt taaattcaaa caacaaaccg atattttaca cttttgaggc atttcatcca
     7021 acaaaacaat tgacagaatc cgatgattac cctaaaattc aaatctttct tgcagcgcac
     7081 ccgtagctca gttggataga gtatctggct acgaaccaga gggtcgggcg ttcgaatcgc
     7141 tccgggtgcg ccagtaagaa aatacaatat gcgcccatcg tctagcggtt aggacatcgc
     7201 cctttcacgg cggtaaccgg ggttcgattc cccgtgggcg tgccaaattc caaatccccc
     7261 gaaataatcc ctcggggatt ttttattgcc tcggcaattc attaccatat ctttacctac
     7321 ccccttcatc agactctcag acgtaatcga atcatattca aacctttgcc gtgcaaaccg
     7381 atatcacgta accggatgcg gtgtccgtcc aacattttac ccgattgaaa cgcctgatat
     7441 attgcacccc atcaacgtgg cattactttt cttaacaatc ccctttgaca gcaactgact
     7501 agggcttttt tatgccatca tcaaatttat agtggattaa ctttaaacca gtacggcgtt
     7561 gcctcgcctt tccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
     7621 ttaattcact ataatatttt ctctcccgat tgaaacaggc gtaacagaat atgcccgaag
     7681 ctccggctgc tttcttgttt accgccgcga tatttagagt ataataccaa atttgagcaa
     7741 tagttctaaa acagttagaa ccatttttca tgagcctgac tgattcgtat actcggagaa
     7801 actgatgcaa aatatttttg accctttggt tattcgtgga aaatccctta cccccatcgt
     7861 gcaaggcggt atgggggtcg gtgtttccgc atcgggttta tccagcgcgg tggcacgtga
     7921 aaacggtatc ggaacgattg ccagtgtgga tttgcgccac cttcacgaag acctactcgc
     7981 cgaatcacaa atcaatccga gtgaagagaa atatacatct ttgaactgta ccgcattaga
     8041 cagggaaatc caaaaagcca aaagcgcttc agagggaaaa ggactgattg cggtcaacgt
     8101 gatgaaggcg gtcaaagacc acgccgcgta tgtccgccag gcttgcgaat caggggcgga
     8161 tgcggttgta atgggtgccg gcctgccttt agacctgccg gaaatgaccg agggctatca
     8221 taaagatgtc gcgctgctgc cgattctgtc cgaatcgcgc ggtattaata tcgtcttgaa
     8281 acgttggatg aaaaaaggca tattgcccga tgcgattgta gtcgaacatc ctgcccacgc
     8341 ggccgggcat ttgggcgcgt caaccgtcga aggcgtaaac gatgctaaat tcgacttcaa
     8401 acgcgtgatt gaggaaacgt ttgaagtttt caaaagttta gggctggaaa gcgaaaaaat
     8461 cccgcttatt ttggcaggcg gtatggcaaa ctttgaaaaa gtcaaaaccg ccctgaaaaa
     8521 ctggggagca tccgccgttc aaatcggtac ggcttttgcc gttaccgaag aaggagatgc
     8581 gcaccttaac ttcaaaaaaa cgcttgctgg tgcggaaact gaaaaagtag tcgaatttat
     8641 gtctgttgcc ggtttgccgg cgcgcggcgt acgtaccaaa ttcctagaca gctatatcag
     8701 gcgcgaaggc aaacttcagg caaacgccaa agccgacccg cgccgctgca cccaaggatt
     8761 aaactgcctg accagctgcg gcctgcgcga cgggctttcc aaagcagggc aattctgtat
     8821 tgatatccag cttgctgccg cattccgtgg agaagtggat aaaggcctgt tcttcagagg
     8881 taaagaccct cttcctttcg gcaatgccat ccgcaccgtc cgcgagacga tacaatatct
     8941 gctgacgggg agcgaacctg ttgcaacgct cggacgctga catcaggtag gatttgattt
     9001 gcaaacaaaa tgccgtctga agggccttca gacggcgttt ttcaggctgc gtttcggaat
     9061 agtgtcgaaa aaacggagag aaataaaaac gtttttatgt aaacaaaacg ccttccgcat
     9121 tgtgtcgggc atacaacaca aatgccgtct gaaacccttc agacggcatt tttcaggctg
     9181 catttcggaa tggtgttaaa aaataaacgg gataagatac atttattttc gtccgataaa
     9241 tcagaccatc acgccgaata atcaaacaat gcctgcccga cattgcatct ggcaaaccaa
     9301 tgcaatgaaa acacggcttt tttatttgct tccggcatta ttgaaaagtt tatccatcgg
     9361 ggtcagatcc accgcattgc cttggctggt aatccatttg ttctcgacgc gccacacgcc
     9421 tgccgcgtcc tccacgcgca cataccagtg gttggtcggc gaaagggttt tgaacaccgc
     9481 ctcatattcc gccctgccgt tctgcgcgct gccgacgggc ttgagggcga cggtttgatc
     9541 gtccgccttg cgggtcgggt gcatcagcag caggttcaaa ggctgtttgc cgtcaaactc
     9601 gccgccgaca aacacttttg ccgcattcat atcgggggaa atgagaacct gcaccccgat
     9661 atgccgtctg acggcttctt catcccgatg aagctggatg tcgatatgtt tgccgtcttt
     9721 ataataatcg tccgtaacca aatctgtcgc gtgctgctgc gcgacaaaaa acatagcgac
     9781 gctggcgatg acgacaaaaa tcggccccgc catcaagatc cacggccaga cgtgtttgta
     9841 ccaaggtttg attggagtgt tttgagacac ggttattctc cgataaaggt tgcatcttct
     9901 tccaagacga ccggcttgcc gtcgggcgcg ccgctttcgc ggtattggaa ggtaaattcg
     9961 atagggtggc tgcctttgtc cgcgtattcc ggaatggtgg acacttggac gggaagggtt
    10021 accgtttcgc gcggggcaac cttgataccg ccttcgggca gcccggtcag ggcgatttcg
    10081 tcaaagcctt tgacacttgc ggtaatgagc tgttcttttt cacttttgtt gatgatacgc
    10141 aggctgtatg cgttttccag ccagcctttg gcgttttcgc gcaccagtac gccacggtct
    10201 ttcaaaatat cgacctcgac cattttgcgc gtggacaaac cggccaggaa ggcaatgata
    10261 actaacgcca acaccgcgcc gtaacctgcc acgcgcggtc tgagcagccg ttttttaatg
    10321 tctttttcag aatattcgtg ttccagcgcg ctttcggtcg tataacggat taatccgcgc
    10381 ggatagccca ttttgtccat aatctcatcg cacgcgtcga tacaggcggc gcagccgata
    10441 cattggtatt gcagaccgtt gcggatgtcg atgccgacgg ggcagacttg gacgcacatc
    10501 gcacagttga tgcagtcgcc caaacccgcc tcttccttat tgaccgtttt cttgcgcgcg
    10561 ccgcgcggtt cgccgcgttc cgcgtcataa gaaacaatca gcgtgtcctt gtcgaacatc
    10621 gcgctttgga aacgtgcata cggacacata tgcaggcata ctttttcacg cataatgtgg
    10681 gcgaagaaga aggtcataaa gccgtaaaac gctgcggcaa acatcgcgcc gccacctgct
    10741 gctccagtga ataaatcggg aacgaactgg cggataggga caaaccagcc tgcaaacgtg
    10801 atgcccgtcc acgcgcagac aaggaaaatc agcaggtatt tggtggcttt gatgcggatt
    10861 ttagtgaaat tccacggcga tttttccagt ttcagccgtt tgtttctatc gccttcgacc
    10921 aggttgtcaa tccacagcat aatttcggtg taaaccgttt gcgggcagga atagccgcac
    10981 cacagtcgcc ctgcaatcgt cgtccaccaa aacagcccga aggcgcaaat catcagcagc
    11041 aaggcaaggt aaatcaaatc gcccaccccc aacgacaatc cgaaaatgaa gaaatgccgt
    11101 tcggggatat tgaaaacgac ggcctgcctg ccgctccagt tgaaccacgg aatgacgtaa
    11161 aacacaaact gcgtcgccaa tacggcggcg atacgcagtt tggcgaaccg tccttccgcc
    11221 tttttgggat ggatgcgttc gccttcggga tggatttgaa tcacgctgac tcgcggatcg
    11281 aatgtttttt ttgctttcgg cgcggctttt gtttgttcgg acgtgccgat tccggatgcc
    11341 ggactgccgg cttggttttc cgtggtcatt ctgcattcct tagatttttg attgatggtt
    11401 tgcccgttac cgccgccgtt tgcttttcag acgtcatttt tcttgttttt taaggcgttg
    11461 tgtttcaagt tttgagaaaa tccgtttttc ccaaaatata tttccgctat tgtacaactt
    11521 tatgcgccgt ccggatgtat ggggcggata catttcccat ccgcatcaaa acgcctggat
    11581 tttaccttac tgcccgaaca aaatccgaat acggttaaaa aaaaagacta aaaaaaccga
    11641 cacccccata tcggcagaac cgacggtgca agctcataaa caaacgctat cgacaatccg
    11701 gcacacaatc tataactttt tatttcaaaa ggaataatgg caggcttcgc ccgcaaatcg
    11761 aaaatccttc cccaccggtc tcctgctgcc gccttccccc gcgtccgccc ttttcttgaa
    11821 agcataagcg aatcgggcga taatcaacgc tttccgatta tccacttatc cgaaacacca
    11881 gcaaggaaaa tacaaaatgt ctcaactggc aaacgcaatc cgcttcctct cggccgatgc
    11941 cgttcaaaaa gccaattccg gccaccccgg cgcgcctatg ggtatggcgg aaatggcgga
    12001 aacattgtgg acgaaattcc tcaatcacaa tcccgccaac cccaaattct acaaccgcga
    12061 ccgcttcgtc ctctccaacg gccacgcgtc tatgctgttg tacagcctgc tgcacctgac
    12121 cggctacaat ctgagcattg aagacttgaa aaacttccgc caactgcaca gcaaaacccc
    12181 cggccatccc gaatacggct ataccgacgg cgtggaaacc acgaccggcc cgttggggca
    12241 agggattgcc aacgcggtgg gcatggcatt agccgaaaaa atccttgccg ccgaatttaa
    12301 taaagatggt ttgaacatcg tcgatcatta cacctacgtc ttcatgggcg acggctgtct
    12361 gatggaaggc gtatcgcacg aagcctgttc gctcgccggc actttaggct tgggcaaact
    12421 gattgttttg tatgacgata acaatatttc cattgatggt aaagtggacg gctggtttac
    12481 cgaaaacatc ccgcaacgct ttgaaagcta cggctggcac gtcgttccta acgtaaacgg
    12541 tcatgacacc gccgccattc aagccgccat cgaagccgca cgtgccgaaa ccggcaaacc
    12601 gtccatcatc tgctgcaaaa ccttaatcgg caaaggcagt gccaacaaag aaggcagcca
    12661 caaaacccac ggcgcacctt tgggcgcgga cgaaatcgaa gccacgcgca aacatttggg
    12721 ctggacttac cccgcctttg aaatcccgca agaaatttac gatgcgtgga atgccaaaga
    12781 acaaggcgcg aaactggaag ccgactggaa cgaactgttc gcgcaatatc aagccaaata
    12841 tcctgccgaa gccgcagaat ttgtgcgccg tatggataaa aagctgccgg acaatttcga
    12901 tgaatacgtt cagacggcat tgaaagaagt gtgcgccaaa gccgaaacca tcgctacccg
    12961 caaagccagc caaaacagca tcgaaatctt ggcaaaagag ttgcctgaat tggtgggcgg
    13021 ttcggccgac ctgaccccgt ccaacctgac cgactggtca aacagcgtct ccgttacccg
    13081 cgacaaaggc ggcaactaca tccactacgg cgtgcgcgag ttcggtatgg gtgcgattat
    13141 gaacggtctt gccctgcacg gcggcatcaa accattcggt gcgaccttcc tgatgttcag
    13201 cgaatacgag cgcaacgccc tgcgtatggc ggcactgatg aaaatcaacc ctgtatttgt
    13261 gttcacccac gattccatcg gtttgggcga agacggtccg acccaccaac ccatcgagca
    13321 aaccgccacc ctgcgcctga ttccgaatat ggacgtatgg cgtccgtgcg acaccgccga
    13381 atctctggtg gcttgggcag aagccgtcaa agccgccgat catccgtcct gcctgatttt
    13441 cagccgtcaa aacctgaaat tccaagcgcg cagcgaacaa caactgaacg acatcaaacg
    13501 cggcggctac gtcatcagcg aagcccaagg caacgcccaa gccgtcatca ttgccaccgg
    13561 ctcagaagtc gagctggctt tggaagcgca aaaagcactc gccgcgcaaa acatcgccgt
    13621 acgcgtcgtt tccatgccgt ccaccaacgt attcgaccgc caagacgccg cctatcaagc
    13681 cgccgtcctg cccgaaagcc tgccgcgcat cgccgtagaa gccggccacg ccgacggctg
    13741 gtacaaatat gtcggactga acggcgcagt cgtcggcatc aaccgcttcg gcgaatccgc
    13801 ccctgccgat ttactcttca aagcattcgg ctttaccgtg gacaatgtgg ttgatacggt
    13861 gaaatccgtg ctgtaacccc acacctaaac aaatgccgtc tgaacgcttt tcagacggca
    13921 tttttttgcc ctcccgcctt gatgcttcct cattccgcca atctccatta taatatttgc
    13981 gaatcactct tattcacatt tcaaaaggag aaacgcatga gcacccgtac cgaacatgac
    14041 accatgggca atgtcgaagt cccgtccgaa gcctattggg gcgcgcagac ccagcgcagc
    14101 cgcaacaatt tcaaaatcgg cggcgaaacc ctgccgcagc cgctgattta tgctttggcg
    14161 ctggtgaaaa aagccgccgc cgcgaccaat gtttccctcg gcaggattaa gccggaacag
    14221 gcggatttga ttacgcaggc ggcggatgat gtgttgaacg gcaggcttga cggacagttt
    14281 cctttagtgg tttggcagac cggctccggc acgcagtcca atatgaacat gaacgaagtg
    14341 ctggcaaacc gcgccaacga aatcgccggt acgggtttgg cggcttatca gcccgtccat
    14401 cccaacgacc atgtgaacca cgcgcaatcg accaacgacg cattcccgac cgctatccac
    14461 gttgccgccg cgattgaaat caaccgccac ctcatccccg ccgtaaaagc cctgcgcgac
    14521 acgttggaca aaaaagccca agctttcgcc cctatcgtca aaatcggccg cacccacttg
    14581 caagacgcga cgccgctgac tttgggacag gaattttccg gctacgtttc ccagcttgat
    14641 cacggtttag gccgtctgaa cgatgcgctt aaagacttgt atgaacttgc tttgggcggt
    14701 acggcggtcg gcacgggttt gaacagccat cccgaatacg ccgaaaaagc tgctgctaaa
    14761 ctcgccgaac tgtctggctt gccgtttgtc agcgcgccga acaaatttga agccttaggc
    14821 ggacgcgatg ccgccgttgc cgcttcgggc gcattgaaaa cgctggcggc aagcctgaat
    14881 aaaatcgcca acgacatccg ctggctggca agcggcccgc gttgcggttt gggcgaaatc
    14941 aaaatccccg aaaacgagcc gggttcgtcc atcatgccgg gcaaagtcaa cccgacccaa
    15001 tgcgaagcaa tgacgatggt gtgctgccaa gtgttcggca acgacgttac catcggtatg
    15061 gcgggcgcgt cgggcaattt cgagctgaac gtctatatgc ccgttatcgc ctacaacctc
    15121 ttgcaatcca tccgcctctt gggcgacgcg tgcaacagct tcaacgaaca ctgcgccgtc
    15181 ggcatcgaac ccgtaccgga aaaaatcgac tatttcctgc accattccct gatgctcgtt
    15241 accgcgttga accgcaaaat cggttacgaa aacgccgcca aagtcgccaa aaccgcctac
    15301 aaaaacaaca aatcgttgcg cgaaaccgcc gttgagctgg gcttgctgac gggcgaagaa
    15361 tttgacgaac tggtcgttcc tgccgatatg gttcatccgc gctaatcttt ccctcaaata
    15421 aaatgccgtc tgaaacctcg ttcagacggc attttccgtt gcctgcgaac tagcggcgtt
    15481 tgaacagcct gtcccccacc gccgccgtaa ccgcaccccc gaccacgacc agtgcgccaa
    15541 cataacccaa accgttcata tccggcgcgg caaaagtatc aggcatcaca taatgcccga
    15601 gcaaagaaaa tattacggta aacacgggga gcaaggttgt taccgcgctg actttggaag
    15661 cctcccaatg tttcaacgcc tcgccgaacg agccgtaacc gattaacgta ttcaagcagc
    15721 aatacgcaaa acaaacccac gccaacgtac cgtccaaact tccgatgtgt gccggttcgg
    15781 caaacggcag gaacacggcg gcacttgccg cataaatcaa cagcagaatc tgttgcggcc
    15841 cgaattgcgc cgacagcagc ttttgcgcca cggcataaca cacccatgcc atactgcctg
    15901 ccgcacacag caacacgccc ttcgcatacg cgcccaaacc cgacaactcg ccgaatttat
    15961 cgttaaaaaa cataagcaaa ccggcaagca gcaaaaccaa gccgattttc tgagcggcag
    16021 tcatccggtc tttaaacacc aacacaccga caacaatcat cgtaaacggc gaaatctgcc
    16081 acaaaacctg cgtcgtggtc ggcgaaatat aatgcagccc ttgggcaatc agcacaaagt
    16141 ttgccgaaat gcccgccacg ccgagcagca gcagcctgaa tgagcaccaa gaaaaatccc
    16201 gccgcttcgg cagccgcccg cccagtgcca gcaaaacaaa caataccgcc gccgccacgg
    16261 taaaacgcac ccacaccagc gtcggcgcat cgacaaactt caatacctgc cgcacggcaa
    16321 tcggcagcgt tccccacgtc atcgccgcca aaagtgccaa cgcgaagcct aggagcggcc
    16381 tttggttttc catcctgatt ttcctatttt taaacaaccg tattgccgga cgatgccggt
    16441 ttgccgcatc gggcaataat ggttcaagcg tttggcgttt aattccaacc ctttgatttc
    16501 aaacaaaccg gctgaagctc ggctattgct tcgcgctatt tgaaaacacc gcctgaattt
    16561 taaaatatag tggattaaca aaaaccagta cagcgttgc
//
LOCUS       DAWHXZ010000021        16486 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000021 DAWHXZ010000000
VERSION     DAWHXZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16486
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.021"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            241..1305
                     /locus_tag="WIE35_000445"
     CDS             241..1305
                     /locus_tag="WIE35_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ4153409.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIVHD
                     DVPTGPGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKEGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1446..1769
                     /locus_tag="WIE35_000446"
     CDS             1446..1769
                     /locus_tag="WIE35_000446"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ4153410.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1841..2164
                     /locus_tag="WIE35_000447"
     CDS             1841..2164
                     /locus_tag="WIE35_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ4153411.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2211..2414
                     /gene="tatA"
                     /locus_tag="WIE35_000448"
     CDS             2211..2414
                     /gene="tatA"
                     /locus_tag="WIE35_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ4153412.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2418..3104
                     /gene="tatB"
                     /locus_tag="WIE35_000449"
     CDS             2418..3104
                     /gene="tatB"
                     /locus_tag="WIE35_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ4153413.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKEIGTDMEGNLHDISNGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANPASDGISDVMPSERSYTSAETLGDDRQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            3118..3888
                     /gene="tatC"
                     /locus_tag="WIE35_000450"
     CDS             3118..3888
                     /gene="tatC"
                     /locus_tag="WIE35_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ4153414.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTEQLKRARPYVIVGAFVIAAVITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3941..4546
                     /gene="maf"
                     /locus_tag="WIE35_000451"
     CDS             3941..4546
                     /gene="maf"
                     /locus_tag="WIE35_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ4153415.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPADINETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAATFLNLL
                     SGTGHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4562..4915
                     /locus_tag="WIE35_000452"
     CDS             4562..4915
                     /locus_tag="WIE35_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ4153416.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4905..6719
                     /locus_tag="WIE35_000453"
     CDS             4905..6719
                     /locus_tag="WIE35_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ4153417.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACKSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     LQNLIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPPALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVLLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6909..7586
                     /locus_tag="WIE35_000454"
     CDS             6909..7586
                     /locus_tag="WIE35_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ4153418.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7599..9005
                     /locus_tag="WIE35_000455"
     CDS             7599..9005
                     /locus_tag="WIE35_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ4153419.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9123..11549
                     /locus_tag="WIE35_000456"
     CDS             9123..11549
                     /locus_tag="WIE35_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ4153420.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHSLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKNLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFDSHPLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11594..11839
                     /gene="rpsP"
                     /locus_tag="WIE35_000457"
     CDS             11594..11839
                     /gene="rpsP"
                     /locus_tag="WIE35_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ4153421.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11855..12364
                     /gene="rimM"
                     /locus_tag="WIE35_000458"
     CDS             11855..12364
                     /gene="rimM"
                     /locus_tag="WIE35_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ4153422.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12364..13113
                     /gene="trmD"
                     /locus_tag="WIE35_000459"
     CDS             12364..13113
                     /gene="trmD"
                     /locus_tag="WIE35_000459"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ4153423.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13129..13494
                     /gene="rplS"
                     /locus_tag="WIE35_000460"
     CDS             13129..13494
                     /gene="rplS"
                     /locus_tag="WIE35_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ4153424.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13815..14540
                     /locus_tag="WIE35_000461"
     CDS             13815..14540
                     /locus_tag="WIE35_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ4153425.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLSRQDIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPSTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            14575..15450
                     /locus_tag="WIE35_000462"
     CDS             14575..15450
                     /locus_tag="WIE35_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ4153426.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15527..16441
                     /locus_tag="WIE35_000463"
     CDS             15527..16441
                     /locus_tag="WIE35_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ4153427.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSSDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALINAMKQ"
BASE COUNT         4282 a         5299 c         3618 g         3287 t
ORIGIN      
        1 ccgtactggt ttttgttaat ccactataaa tgccatctga agccgattcg gttttcagac
       61 ggcatcatta tttgacctga cgcaaacaga aagccttccg cgtgcttaaa cacatctctt
      121 gtagtaaaat atctaaacag attcaactgg aaacggtcag gcttcttcac gccattccca
      181 tttcaaccgc cgcctgccag attcagacgg tattcctact tgaaaacaag gagcgttaca
      241 atgaaagcag cacgttttta cgacaaaggc gacatccgca tcgaagacat ccccgaaccg
      301 accgtcgccc ccggcaccgt cggcatcaat gttgcctggt gcggcatctg cggtaccgac
      361 ctgcacgaat tcatggaagg cccgattttc attccgcctt gcggtcatcc gcacccgatt
      421 tccggcgagt ccgcacccgt cacgatggga cacgagttct ccggcgtggt ctatgccgtc
      481 ggcgaaggcg tggacgacat caaagtcggt caacacgtcg ttgtcgagcc ttacatcgta
      541 cacgacgacg taccgaccgg cccgggcagc aactaccacc tctctaaaga catgaacttc
      601 atcggcttgg gcggctgcgg aggtggcttg tctgaaaaaa tcgctgtcaa acgccgttgg
      661 gtgcatccga tttccgacaa aatcccgttg gatcaagccg ctttgatcga accgctgtct
      721 gtcggacacc acgcctatgt acgcagcggc gcgaaagaag gcgatgtcgc attggtcggc
      781 ggtgcaggcc cgatcggttt gctgttggct gccgtgctga aagccaaagg catcaaagtc
      841 atcatcaccg agttgagtaa agcacgcaaa gacaaagcgc gcgaatccgg cgttgccgac
      901 tacatcctcg acccgtccga agtcgatgtt gtcgaagaag tgaaaaaact gaccaacggc
      961 gaaggcgtgg acgtggcatt cgaatgtacc agcgtgaaca aagtgttgga tactttggtc
     1021 gaagcctgca aacctgccgc caatttggtt atcgtatcca tctggagcca ccctgcaacc
     1081 gtcaacgtcc acagcgtcgt gatgaaagag ctggatgtgc gcggcacgat tgcctactgt
     1141 aacgaccacg ccgaaaccat caaactggtc gaagaaggca aaatcaacct cgaacccttc
     1201 atcacccagc gcatcaagct ggacgaactg gtttccgaag gcttcgagcg tctgatccac
     1261 aacaacgaat ccgccgttaa aattattgtg agtccaaacc tgtaagcaaa ttacatttgc
     1321 aacagcacaa atgccgtctg aaacgctcag acggcatttt ccaacgaagc gcaacaggcg
     1381 tattgcccga gagcagccgt ttagcctatc attccgaaca aacagaaaca aacagaagga
     1441 aaatcatggg agattccgta ctatccgcca tccaacaaac catcacccag cgaaaatctg
     1501 ccaatccgtc cgaatcttac gtcgcacagc tcttgcataa gggcgaagac aaaatcctaa
     1561 aaaaagtgat tgaagaggcg ggcgaagtgt tgatggcatc caaagacaaa aacccgtccc
     1621 acctggttta cgaagttgcc gacttatggt ttcacaccat gattcttctg acacaccacg
     1681 acctgaaggc ggaagacgta ttggacgaac ttgcgcgccg ccaaggtttg tcgggcttgg
     1741 ccgaaaaagc cgctcgcaca gaatcttgaa tttatattaa aatccgcact ttcccacatt
     1801 caatccgtct gaccgctttt cagacggcat cggagccgtt atggacaact gtattttctg
     1861 caaaatcgcc gccaaagaca ttccggcgca aaccgtctac gaagacggcg aaatggtttg
     1921 tttcaaagac atcaaccccg ctgctccggt tcatctgctg ctgattccca aagtccattt
     1981 cgattcgttg gcacacgccg cgcccgaaca tcagcccctt ttgggaaaaa tgatgctgaa
     2041 agttcccgaa atcgccaaag cggcaggact ggcagacggc ttcaaaaccc tgatcaacac
     2101 cggaaaaggc ggcggacaag aggtcttcca cctgcatata cacatcatgg gcacacccgt
     2161 ataaaccgtt atttcacaat caacccctaa tacttactta aggatacatc atgggcagtt
     2221 tttctctgac gcactggatt atcgtactga ttatcgtcgt tttgatattc ggcaccaaaa
     2281 aactgcgcaa cgtcggcaaa gacctcggcg gtgcggttca tgacttcaaa caggggctga
     2341 acgaaggcac agacggcaaa gaagcccaaa aagacgatgt aatcgaacac aaaaaagacg
     2401 aagacaaagc gtaatttatg tttgatttcg gtttgggcga gctggttttt gtcggcatta
     2461 tcgccctgat tgtcctcggc cccgaacgcc tgcccgaggc cgcccgcacc gccggacggc
     2521 tcatcggcag gctgcaacgc tttgtcggca gcgtcaaaca ggaatttgac actcaaatcg
     2581 aactggaaga actgaggaag gccaagcagg aattcgaagc tgccgccgct caggttcgag
     2641 acagcctcaa agaaatcggt acggatatgg agggtaatct gcacgacatt tccaatggtc
     2701 tgaagccttg ggaaaaactg cccgaacagc gcacgcctgc tgatttcggt gtcgatgaaa
     2761 acggcaatcc gcttcccgat gcggcaaacc ccgcatcaga cggcatttcc gacgttatgc
     2821 cgtccgaacg ttcctacact tccgccgaaa cccttggaga cgacaggcaa accggcagta
     2881 cagccgaacc cgcggaaacc gaccaagacc gcgcatggcg ggaatacctg actgcttctg
     2941 ccgccgcacc cgtcgtacag accgtcgaag tcagctatat cgataccgct gttgaaaccc
     3001 ctgttccgca taccacttcg ctgcgtaaac aggcaataag ccgcaaacgc gatttgcgtc
     3061 ccaaatcccg cgccaaacct aaattgcgcg tccgtaaatc ataaagaggg caatccggtg
     3121 tccgaaacac aaaacgaaca acccgtccaa ccgcttgtcg agcatctcat cgagctgcgc
     3181 cgccgcctga tgtggacggt tgtcggcatc ttagtctgct ttttcggcct aatgccgttt
     3241 gcccaacaac tctatacttt tatcgccgac ccgctgatgg caaacctgcc caaagacacc
     3301 agcatgattg ccaccgatgt catcgcaccg tttttcgtgc cggtcaaagt taccctgatg
     3361 gcggcatttt taatttcgct gccgcatacg ctctaccaaa tctgggcatt tgtcgcgccc
     3421 gcactctacc aaaacgaaaa acgcctgatt acgccgctcg tcctctccag cgtcagcctg
     3481 tttttcatcg gcatggcatt tgcctacttt ttggttttcc ccgtcatttt caaattcctt
     3541 gccagcgtta cccctgtcgg tgtcaatatg gcgacagaca tcgacaaata cctctccttc
     3601 atcttgggga tgtttgtcgc attcggtaca acgtttgaag tccccattgt cgttatcctg
     3661 ttaaccaaaa ttggtgtggt aacaaccgaa cagctcaaac gcgcccgccc ctatgtgatt
     3721 gtcggcgcgt ttgtcattgc cgcagtcatc acaccgcccg atgtgatttc acaaaccctg
     3781 cttgccattc cgctgattct cttatacgaa gcaggtattt ggttcggacg ctttttcacg
     3841 ccacgttcag aacaggatgg cgacatacag ccgcctgcaa caacctgaca ctatgccgtc
     3901 cgaacctccg cctcataccg caacagatta aggaatacct ttgaataccc tctatttagg
     3961 ttcaaacagc ccgcgccgaa tggaaatcct gacacagttg ggctatcagg tcgtcaagct
     4021 gcctgccgac atcaatgaaa cggtcagaca gaacgaagac cctgcccgtt acgttcaaag
     4081 gatggcagaa gaaaaaaacc gaaccgccct gaccctcttt tgcgaaacca acggcacaat
     4141 gcccgatttc cccctgatta ccgccgacac ctgcgtcgtt tcagacggca tcatattggg
     4201 caaaccccgc tcccaagctg aagcggcaac ctttctaaac ctgctgtcgg gaaccgggca
     4261 taccgtcctg actgctgtct gcattcatta tcgcggcaaa acgtcaagcc gcgtccaaac
     4321 caaccgcgtc gttttcaagc ccctgagttc ggaagaaatt tccgcctatg tccaaagcgg
     4381 cgaaccgatg gacaaagccg gcgcctacgc cgtacaaggc ataggcggca tctttatcca
     4441 atctatcgaa ggcagcttca gcggcattat ggggctgccc gtttatgaaa ccgtttcgat
     4501 gttacaggat ttgggatacc gtaccccacc tttcatccga gcctaacccg taaagaccgc
     4561 cgtgaacaga caaaccgctt acctccttac ctctttcagc ctgatcgcac tgataatcct
     4621 gtccctttcc tgggaactgt ggattgcacc gttgcgcccg ggcggctcgt ggctcgcgct
     4681 caaagccctc cccctctgtc tgccgctttc aggcatcttg aaaaagaaaa tctataccta
     4741 ccaatacagc tccatgctgg ttctgattta ctttgccgaa gccgtcatgc gcctgttcga
     4801 cgcctatccc gcagaacaga tttgcgccgc gctttccgca gtattcagca tcatcttctt
     4861 catatcctgc ctgtcgttcg tcaaacaata caaggaaaca aacaatgccc gctgaaacga
     4921 ccgtatccgg cgcgcacccc gccgccaaac tgccgattta catcctgccc tgcttccttt
     4981 ggataggcat cgtccccttt accttcgcgc tcaaactgaa accgtcgccc gacttttacc
     5041 acgatgccgc cgccgcagcc ggactgattg tcctgttgtt cctcacggca ggaaaaaaac
     5101 tgtttgatgt caaaatcccc gccatcagct tccttctgtt tgcaatggcg gcgttttggt
     5161 atcttcaggc acgcctgatg aacctgattt accccggtat gaacgacatc gtctcttgga
     5221 ttttcatctt gctcgccgtc agcgcgtggg cctgcaagag tttggtcgca cacttcggac
     5281 aagaacgcat cgtgaccctg tttgcctggt cgctgcttat cggctccctg cttcaatcct
     5341 gcatcgtcgt catccagttt gccggctggg aagacacccc cctgcttcaa aacctcatcg
     5401 tttacagcgg gcaaggcgta atcggacaca tcggacagcg caacaacctc ggacactacc
     5461 tgatgtgggg catactcgcc gccgcctacc tcaacggaca acgaaaaatc ccgcccgcct
     5521 tgggcgcaat ctgcctgatt atgcagaccg ccgttttagg tttggtcaac tcgcgcacca
     5581 tcttgaccta catagccgcc atcgccctca tccttccctt ctggtatttc cgttcggaca
     5641 aatccaacag gcggacgatg ctcggcatag ccgcagccgt actcctaacc gcgctgttcc
     5701 aattttccat gaacaccatt ctggaaacct ttaccggcat ccgctacgaa actgccgtcg
     5761 aacgcgtcgc caacggcggt ttcacagact tgccgcgcca aatcgaatgg aataaagccc
     5821 ttgccgcctt ccagtccgcc ccgatattcg ggcacggctg gaacagtttt gcccaacaaa
     5881 ccttcctcat caatgccgaa cagcacaaca tacacgacaa cctcctcagc aacttgttca
     5941 cccattccca caacatcgtc ctccaactcc ttgcagagat gggaatcagc ggcacgcttc
     6001 tagttgccgc aaccctgctg acgggcattg ccgggctgct gaaacgcccc ctgacccccg
     6061 catcgctttt cctaatctgc acgcttgccg tcagtatgtg ccacagtatg ctcgaatatc
     6121 ctttatggta tgtctatttc ctcatcccct tcggactgat gctcttcctg tcccccgcag
     6181 aggcttcaga cggcatcgcc ttcaaaaaag ccgccaatct cggcatactg accgcctccg
     6241 ccgccatatt cgcaggattg ctgcacttgg actggacata cacccggctg gttaacgcct
     6301 tttcccccgc cactgacgac agtgccaaaa ccctcaaccg gaaaatcaac gagttgcgct
     6361 atatttccgc aaacagtccg atgctgtcct tttatgccga cttctccctc gtaaacttcg
     6421 ccctgccgga ataccccgaa acccagactt gggcggaaga agcaaccctc aaatcactaa
     6481 aataccgccc ccactccgcc acctaccgca tcgccctcta cctgatgcgg caaggcaaag
     6541 ttgcagaagc aaaacaatgg atgcgggcga cacagtccta ttacccctac ctgatgcccc
     6601 gatacgccga cgaaatccgc aaactgcccg tatgggcacc gctgctaccc gaactgctca
     6661 aagactgcaa agccttcgcc gccgcgcccg gtcatccgga agcaaaaccc tgcaaatgac
     6721 ccgcgccggc ggatgcggat accgcccgaa atgtaaagct ccatgcaaga cattgcaaaa
     6781 aacagcaaac cggtagggaa atacgctatc aaaaaacatt gcagccgtgt taagataaac
     6841 cgtcaaacaa tcttttcacg ccccgcccga aacagggtcg gggcataccc ttacgaaaag
     6901 gaaacaccat gagccgcgta ttactcgtag atgacgatgc cctgctaacc gaactgctga
     6961 ccgaatacct gagcgccgaa ggtctgaacg tccgcagcgt tcccgacggg gaagcaggcg
     7021 tacaggaaat cctgagcggg cagtacgatg tagtcgtatt ggattccatg atgcccaaaa
     7081 tgaacggctt ggatgtcttg aaaaacgtac gcgcccgaag caccgtcccc atcatcatgc
     7141 tgaccgccaa aggcgacgac atcgaccgaa tcatcggctt ggaaatgggc gcggacgact
     7201 atgtccccaa accctgcaca ccacgcgaac tcttggcacg catcaatgcc atcctgcgcc
     7261 gcgcacaaca cagcggcgaa cagaacaacg cacccaacag catctccgtc agcgatgtcg
     7321 tcctgtaccc cgccaaacgc caggcatccg tcaaagacat gccgctcgaa ctgaccagca
     7381 ccgaattcaa cctgctcgaa gtcctgatgc gccatgccgg acaggtagtc agcaaagaaa
     7441 ccctgtccgt cgaagcactc gaccgcaagc tggcaaaatt cgaccgcagt atcgacgtac
     7501 acatctccag catccgccac aagttgggcg atgcctctct gattcaaacc gtacgcggct
     7561 tgggctacct gtttgtcaaa aactgaagca acgggcaaat gaaactgttc caacgcattt
     7621 tcgccacatt ttgcgcagtt atcgtctgtg caatctttgt ggcgagtttt tctttttggc
     7681 tggtgcagaa cacccttgcc gaaaaccagt tcaaccaacg ccgcaccatc gaaaccactt
     7741 tgatgggcag catcatttcc gcattccggg cacgcgggga cgcgggcgcg cgcgaaatcc
     7801 tgacggaatg gaaagacagc cccgtctcat cgggcgtgta cgttatacag ggcgacgaga
     7861 aaaaagatat cctgaaccgg tatatcgaca gctacaccat cgaacgcgcc cggcttttcg
     7921 ccgccggaca cccgcattcc aacctcgtcc atatcgaata cgaccgcttc ggcgaagaat
     7981 acctgttctt caccaaagac tgggacaaac tccaagcccg ccgcctgccc agccccctgt
     8041 tgatccccgg tctgccgctc gccccgattt ggcacgaact catcatattg tccttcatca
     8101 tcgtcgtcgg actgctgatg gcgtacatcc tcgccggcaa tatcgccaaa cccatcagaa
     8161 tcttaggcaa cggcatggac agggtggcaa acggagaact tgaaacccgt atctcccaac
     8221 aggtcgacga ccgcgacgac gaattgtccc atcttgccat ccaattcgac aaaatggtgg
     8281 aaaaacttga aaaactcgtt gccaaagaac gccacctgct ccatcacgtc tcccatgaaa
     8341 tgcgttctcc ccttgcgcgc atgcaggcaa ttgtcggact gattcaggcg cagccccaaa
     8401 aacaggagca atatctcaaa cggctggaag gcgaactgac ccgcatggat acgctggccg
     8461 gggaactgtt aaccctgtcc cgtctcgaaa cttccaatat ggctttggaa aaagaaagcc
     8521 tgaaactcct gcccttcctg ggcaacctgg tagaagacaa tcaaagcatt gcccagaaaa
     8581 acggacaaac ggttaccctg tctgccgacg gaaaaatccc cgaaaataca accatccttg
     8641 ccaacgaaag ctacctgtac cgcgccttcg acaacgtcat ccgcaacgcc gtcaactaca
     8701 gtcccgaagg cagcaccatc ctgatcaaca tcggacaaga ccacaaacac tggataatcg
     8761 acgttaccga caacggcccc ggcgtggacg aaatgcagct cccgcacatc ttcaccgctt
     8821 tctaccgtgc agactccagt gcgaacaaac ccggaacagg actggggctt gcattgaccc
     8881 aacatattat tgaacagcac tgcggaaaaa tcatcgccga aaacatcaaa ccgaacggtc
     8941 tgcggatgcg cttcatcctg cccaagaaaa aaaccggttc caaaacagaa aaaagtgcga
     9001 actgaccata atgccgtctg aaccggcttc agacggcatt gcacaaacag ttaccccaaa
     9061 acaaacacac agcgggtgca tctccaacaa tcacgcacat accgtccgca aaggaatata
     9121 tcatgtcggc acaaaccgat ccgggctact ttttcatgcc caaccacatt attctgatag
     9181 gcgcgagcga acgtccgtac agcctgggcg aacgcgtact cagcaacctg ctgagtacgc
     9241 cctttcaagg aaaaatcacc cccgtaaacc cgcgccacca caccatagcc gggcttcccg
     9301 cctacaccag cctcaacaaa atccccggca atgcagacct gattattgcc gttaccctac
     9361 ccgacagtta cgacaccctc ttcaaaacct gccgcaaaaa gcagctccga cacatcatcc
     9421 tcatacagga ctgggacagc ctgtctgccg cagaactgca caccgccgaa actgccatcc
     9481 gcaaacacca cggcaacgga cttaacatca ccgcctgcac caccgcaggc atccaactgc
     9541 cctcactcgg actcaacatc agtacccaag acggatacgc cgcaggccat accgccatac
     9601 tgaccggcaa tgccgccgtc agccgtcaaa tcgacaacat cctgaacaaa ctccgtcaag
     9661 gcacatcccg ccacatcagc ctgcatcccg gcatcagccc catcacatcc gccgattggc
     9721 tcaaccgctt cggacacagc ctgcacacca aaaccgccgt cctacaccac aaccctgaag
     9781 aagaccagcg caaactgttc agcgcaatcc gccaatttac ccgccatacg ccgctgattc
     9841 tccacatcac ctgccgcacg acagaaaccg accgtgccgt actgcacagc ctcgcccgac
     9901 actgcaactt cctcatcagt ttcaacgccg acgacctcga agccgcactg cgcgcccaac
     9961 tgtccgacct tcctcccctg tcccgactcg acatcctgtc cgacacgcct gccgaatggc
    10021 tgaacgcgca cgcgccaaaa aacctcgccc tccactttcc caaccttccc caccacatcc
    10081 gcaacggaca cctgaccggc acacccacac cctcaatctg ccacgacatc gcctcacgtc
    10141 agcttgcccg ccccgacacc caagccgtcc taaccatcct cagtccctcc ggacacgagg
    10201 attacaaaaa aacagcacgc gcccttatcc gcctgtccga acagaccgcc aaacccctgc
    10261 tcgtcagcag ccccttttca gacggcataa cacatttcga cacccccact caggcaatcc
    10321 gcaccctttc ctaccgcaac accgccgccg ccctgaaaca ggcacagctc gacattgcac
    10381 cgccgcagcc atgccgtctg aaaacaccgc aaccccaaaa catcaaaaaa gcccttgcag
    10441 cggcaaaccc ctccctgctc gccgaagccc tgcacctccc cccctaccgg cacaccaccc
    10501 ataacgccgt acaattccaa ttcgacagcc accccctcta tggcgacatc ctgaccgcac
    10561 gctgcaacgg acaaaccact gccgtactcc cgccgtttac cacgctcgac agccgccacc
    10621 ttgcccgctt tgccgaactc gacggcacac aaaccctcga ccagttcctg cacacactga
    10681 ccatcattcc cgaataccgc caacacattc tcggcatcac cctcaaccta aacggcggac
    10741 aatacagcag cgatttcatc ttaaagacac ccgaaacaca cgacacgccc aaacgcaaga
    10801 acacaggcaa agccgcccaa accctcgaac atgccgccgc aaaaatgcag agtgccgccg
    10861 catacctgaa acacaaaaac ccgacagccg ccgaatttct ccgccacaca agcgaagccg
    10921 ccgcagaact gctcggcagc aaaaccgaaa ccggagcagc cgtacccaac gtacttgccc
    10981 cctatcccgc agcatacccc aaaacactgt ccctaagaaa caacacgacc gttaccatta
    11041 cccccatttt gcccgaagac gcagaagcca aacagcagtt cgtccgcagc ctcggtcccg
    11101 aagcacggta cacacgcttc atgacccaca ccaacgaact gcccgcagcc acgttggcac
    11161 gcctgtgcaa ccccgattac cactgtgaag ccgcatggac ggcaaaggat gccgacagca
    11221 acatcgtcgc cgtcgtccgc cacagccgcc tgaatcgcaa cgaatgcgaa ttcggcatca
    11281 cactggcgga acatatgcgc ggcagcggac tggcacagaa aatgatggaa ctcatcatcc
    11341 aaaccgccgc acagcaaggc taccggacta tgagtgccga cattctcaaa accaataccc
    11401 ccatgatcaa acttgctgaa aaatcaggat ttaccctcaa ggaatcggac accgaaaaaa
    11461 acctgtaccg cgcatatctg aaccttgcgg cagacaaaac aacagaaaaa acaaataaaa
    11521 acttgcgcac cgaccacaaa ataacctaaa atcggcagtt tccatatatc cgattttctc
    11581 aaaaggactc aaaatggtag ttatccgttt ggcacgcggc ggctcgaaac accgcccctt
    11641 ctacaacgtc atcgttactg actcacgcag ccgccgcgac ggccgcttca tcgaacgcgt
    11701 aggcttctac aaccccgtag ccaacgaaaa acaagagcgc gtccgcctca atgcagaccg
    11761 cctgaaccac tggattgcac aaggcgcgca agtcagcgac tccgttgcaa aactgattaa
    11821 agaacaaaaa gccgcctaat ccgcatttgc cgccatgaca gacactcaaa accgggtagc
    11881 catgggctac atcaaaggcg tattcggcat aaaaggctgg ttgaaaattg ccgccaacac
    11941 cgaatattcc gacagccttt tggactaccc cgagtggcat ttggtcaagg acggcaaaac
    12001 catcagcgtt acccttgaag ccggaaaagt cgtcaacggc gaactccaag tcaaattcga
    12061 aggcataaac gaccgcgact tggcattctc attgcgcggt tacaccatcg aaataccccg
    12121 tgaagcattc gccccgacag aagaagacga atactactgg acagacttgg tcggcatgac
    12181 cgtcgtcaac aaagaccata ccgttttagg caaggtaagc aacctgatgg aaaccggcgc
    12241 aaacgacgta ttgatgattg acggagaaca cgggcagatt ctgattccgt tcgtttccca
    12301 atatatcgaa accgtcgata ccggcagcaa gattattact gccgactggg gtttggacta
    12361 ctgatgctta tccaggcagt taccattttc cccgaaatgt tcgacagcat tacccgctac
    12421 ggcgtaacgg gacgcgcgaa cagacaggga atctggcagt ttgaagcagt caatccccga
    12481 aagtttgccg acaacagatt gggctacatc gacgaccgcc cgttcggcgg cggcccggga
    12541 atgattatga tggctccgcc gcttcatgca gcaatagaac acgccaaagc acaatcttcc
    12601 caagctgcaa aagtcattta cctcagcccc caaggaaaac cgctgacaca cccaaaagcg
    12661 gcagaactgg cagaacttcc gcatctgatt ctgctgtgcg gacgctatga gggaatagac
    12721 gaaagactgc tgcaaaccag cgtcgatgaa gaaatcagca tcggagactt cgttgtttcc
    12781 ggcggagagc ttcccgccat gatgctgatg gatgcggtat tgaggctcgt acccggcgta
    12841 ttgggcgata tgcagtctgc cgaacaagat tcgttctcaa gcggcatttt ggactgcccc
    12901 cactacacca aacccttaga atttcaaggc atggctgttc cggaagtatt acgttccggc
    12961 aatcatggct tgatagcgga atggcggttg gaacaatcgc tgcgccgcac cttggagcgc
    13021 agacccgatc ttttggaaaa gcgcgtttta atcccaaagg aatcccgcct cttagaaacc
    13081 atccggcaag agcaacggga aatccaatca taatttagga aaaaaacaat gaacctgatt
    13141 caacagctcg agcaagaaga aattgcccgc ctgaataaag aaatccccga attcgcaccg
    13201 ggcgacaccg tagtcgtatc cgtacgcgtc gtggaaggta cccgcagccg tctgcaagcc
    13261 tacgaaggcg tggttattgc ccgtcgcaac cgtggtctga acagcaactt catcgtccgc
    13321 aaaatctcca gcggcgaagg tgttgaacgt actttccaac tgtactctcc gaccgtcgaa
    13381 aaaatcgaag tcaaacgccg tggcgacgta cgccgtgcca aactgtacta cctgcgcggc
    13441 ctgaccggta aagctgcacg catcaaagaa aaactgcctg cacgcaaagg ttgattcaaa
    13501 ccgttttccc caatgccgtc tgaaccttct gacggcattt cttattgccg caacatccgc
    13561 aacggaacag caccaagaag cacacccctt ccggcacatc ccgaatcccc gtccgaaccg
    13621 aacaaaattt acagaccaaa cgttatacag tatcatatca gccatataat aataaccgct
    13681 ttaccgcttt ccctttcaaa accgcaaatc atcatgacac cttccctttt actatcagga
    13741 ttgaccttcc gcctcgtcct tgccctgatt gccgtatccc ttttatgggg cgtttacttt
    13801 tgggcagtat ccgcatgagc atcattgtcg aaaacctgac cgtcagctac cgccgccgtc
    13861 ctgccgtaca ccacgttgac attacttttg aagaacatag tatgtgggcg gttttcggtc
    13921 ccaacggcgc agggaaatcc acctttctca aatccttgat gggattgcag cctatcgata
    13981 caggcagcat ccggctggat ggattgagcc gccaagacat cgcctacctt ccccagcagt
    14041 ccgatatcga ccgctcccaa cctatgactg ttttcgactt ggcggcaatg gggctttggt
    14101 atgaaatcgg ctttttcaaa gggataaata ccgctcaaaa acaacgcgtt cacgaagcct
    14161 tggagcgtgt aggaatgcgg cagtttgcac gccgccaaat cgcccacctc tcaaacggac
    14221 aattccaacg cgtccttttt gcccgaatgc tggttcaaaa tgcaaaattc ctgcttttgg
    14281 acgaaccttt caacgccgtt gatgcacgga cgacctacga acttctcgac gtattgcaga
    14341 aatgccattg cggcggacac gctatcattg ccgtactgca cgattacgaa caagtccgtg
    14401 cctactttcc cagtaccctg ctgctcgccc gagaaaaaat tgcggcaggc gcaaccgaga
    14461 ccattctgac agaaccctac ctcgcccaag ccaacgccaa aatgcagcaa caggaaagcc
    14521 ccgactggtg cgcctcataa atactgcccg aaaccacgcc ccacaccaga caccatgaat
    14581 ctctacgacc tgctccttgc cccctttgca gaattcgact ttatgcgcta cgccctcgca
    14641 tccgtcttct gcctgtccct cagtgccgca cccgtcggcg tattcctcgt catgcgccgt
    14701 atgagcctga taggcgacgc attgagccac gccgtcctgc ccggtgccgc cgtcggctac
    14761 atgtttgccg gcttgagcct gcccgccatg ggtttgggcg gcgtagccgc aggcatgctg
    14821 atggcactgc ttgccggact cgtcagccgc ttcaccaccc tgaaagaaga tgccaacttt
    14881 gctgcctttt atctcagcag cctcgccatc ggcgtagtcc tcgtcagcaa aaacgggagc
    14941 agcgtcgatt tgctccacct ccttttcggc tctgtacttg ccgtcgatat tcctgccctg
    15001 cagctcatcg ccgccgtctc cagcctcacg ctcattaccc ttgccgtcat ctaccgcccg
    15061 ctcgtactcg aaagcatcga ccccctgttt ctcaaatccg tcggcggcaa aggcgggctt
    15121 tggcacgtcc tctttctcgt cctggtcgtc atgaacctcg tatccggctt tcaagccctc
    15181 ggcacactca tgtccgtcgg actcatgatg ctgccagcca ttaccgcccg cctgtgggcg
    15241 aagcacatgg gcgcactcat cctcctatcc gttctgacag ccctgctgtg cggcttgagc
    15301 ggactgctca tttcctacca catcgaaatt ccctccggtc ccgccatcat cctctgttgc
    15361 agcgtccttt atctcttttc cgtcatactc ggcaaagaag gcggcattct gaccaaatgg
    15421 ctcaaaaacc accgccacca caccacctga ccccgcaagc cgcttgaaca aacaggcagc
    15481 ttgttttacc ataacagatg ttacgttata tcacaggaga acagttatga aacacctcaa
    15541 actcaccctt attgccgcat tgctaaccac cgccgcaact gccgcccccc tgcccgtcgt
    15601 aaccagcttc agcattttag gcgacgttgc caagcaaatc ggcggagagc gcgtatccat
    15661 acaaagtttg gtcggagcca accaagatac tcatgcctat cacatgacca gtagcgacat
    15721 taaaaaaatc cgcagtgcaa aactcgtcct gctcaacggc ttaggacttg aagctgccga
    15781 tgtacaacgt gccgtcaaac agagcaaagt atcctatgcc gaagcgacca aaggcatcca
    15841 acccctcaaa gccgaagaag aaggcggaca ccatcatgac cacgaccatg accacgaagg
    15901 acaccaccat gaccacggcg aatttgaccc gcacgtctgg aacgaccccg tccttatgtc
    15961 cgcctatgcc caaaacgtcg ccgaagccct gataaaggcc gaccccgaag gcaaagttta
    16021 ttatcaacaa cgcttgggca actaccaaat gcagcttaaa aaactgcaca gcgacgcaca
    16081 agccgcattt aacgccgtcc ctgccgccaa acgcaaagtc ctgaccgggc acgatgcctt
    16141 ttcctacatg ggcaaacgtt accatattga attcatcgcc ccgcaaggtg tgagcagcga
    16201 agccgagcct tcagccaaac aagtcgccgc catcatccga caaatcaaac gcgaaggcat
    16261 caaagccgtc tttaccgaaa acatcaagga cacccgtatg gttgaccgta tcgccaaaga
    16321 aaccggtgtc aacgtcagcg gcaaactgta ttccgacgca ctcggcaacg cgcccgcaga
    16381 cacctacatc ggcatgtacc gccacaacat caaagccttg atcaacgcga tgaagcaata
    16441 aaaccgtttg attggtttga atttgaaaag gcaaaaggcc gtctga
//
LOCUS       DAWHXZ010000022        16430 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000022 DAWHXZ010000000
VERSION     DAWHXZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16430
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.022"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..335
                     /locus_tag="WIE35_000464"
     CDS             <1..335
                     /locus_tag="WIE35_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ4153428.1"
                     /translation="KFKPYIGARVAYGHVKHQVHSVKKETTTTFLAPTQGATVPGKIV
                     EGPFSKPAYHESHSISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEV
                     SLGMRYHF"
     gene            complement(309..518)
                     /locus_tag="WIE35_000465"
     CDS             complement(309..518)
                     /locus_tag="WIE35_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153429.1"
                     /translation="MPSERLSDGIFPCVLNRIHETLDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIGIGESEVVAHAQ"
     gene            complement(536..1093)
                     /locus_tag="WIE35_000466"
     CDS             complement(536..1093)
                     /locus_tag="WIE35_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ4153430.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1169..2803)
                     /locus_tag="WIE35_000467"
     CDS             complement(1169..2803)
                     /locus_tag="WIE35_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ4153431.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3081..4187)
                     /gene="serC"
                     /locus_tag="WIE35_000468"
     CDS             complement(3081..4187)
                     /gene="serC"
                     /locus_tag="WIE35_000468"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ4153432.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGATVVIIRED
                     LLERCPNDIPDVFNYRSHINRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTEDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4552..4983
                     /gene="rimP"
                     /locus_tag="WIE35_000469"
     CDS             4552..4983
                     /gene="rimP"
                     /locus_tag="WIE35_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ4153433.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            5011..6513
                     /gene="nusA"
                     /locus_tag="WIE35_000470"
     CDS             5011..6513
                     /gene="nusA"
                     /locus_tag="WIE35_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ4153434.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGVDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6526..9414
                     /gene="infB"
                     /locus_tag="WIE35_000471"
     CDS             6526..9414
                     /gene="infB"
                     /locus_tag="WIE35_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ4153435.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDTAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKTAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9625..11019)
                     /locus_tag="WIE35_000472"
     CDS             complement(9625..11019)
                     /locus_tag="WIE35_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ4153436.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLSGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(11093..12433)
                     /locus_tag="WIE35_000473"
     CDS             complement(11093..12433)
                     /locus_tag="WIE35_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ4153437.1"
                     /translation="MLNPSRKLVELIRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRETLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAKCGAAWLEPDRAAQEGRLKDQ"
     gene            12576..13202
                     /locus_tag="WIE35_000474"
     CDS             12576..13202
                     /locus_tag="WIE35_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ4153438.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYVVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13266..14684)
                     /locus_tag="WIE35_000475"
     CDS             complement(13266..14684)
                     /locus_tag="WIE35_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ4153439.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAVRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            15011..15739
                     /locus_tag="WIE35_000476"
     CDS             15011..15739
                     /locus_tag="WIE35_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ4153440.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     SNPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15798..16286)
                     /locus_tag="WIE35_000477"
     CDS             complement(15798..16286)
                     /locus_tag="WIE35_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ4153441.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWSLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
BASE COUNT         4301 a         4670 c         4198 g         3261 t
ORIGIN      
        1 ataaattcaa accctatatc ggtgcgcgcg tcgcctacgg acacgttaaa catcaggttc
       61 attcagtgaa aaaagaaacc acgactactt tccttgcacc aacgcaaggc gctacagtgc
      121 caggcaagat cgtagaaggt ccgttcagca aacctgccta tcacgaaagc cacagcatca
      181 gcagcttggg tcttggtgtc atcgccggtg tcggtttcga catcacgccc aagctgactt
      241 tagacaccgg ataccgctac cacaactggg gacgcttgga aaacacccgc ttcaaaaccc
      301 acgaagtctc attgggcatg cgctaccact tctgattccc cgataccgat gccgtctgaa
      361 ccttcagacg gcatgagacc tttgcctgcg tacttggtgc gctggtcgcc tccgaacatg
      421 gcgcgacacc cgacatttcc cccgaacgca tcgagcgttt catgaatccg gtttaaaacg
      481 catggaaaaa tgccgtctga aagcctttca gacggcattt cgcttgagat tccgtttacc
      541 aatggctgac aaacgcttcc aaatcggtat tcttgggctt atgcacttcc tctgtcggcg
      601 tgccgaccat catcagcccg atgattttat ccttatccgc acatccgaaa gcctcacgca
      661 acagggggct gttgacccac atccccgtaa tccagacatt gtcgaaacct tgcgccgttg
      721 ccgccagttg cagcgcatac gccgcacaac ccgccgtcag catctgctcc cattccggtt
      781 tcggcttagg cacatcgcgg ttcggcgcaa acgttacacc aatgaccatc ggcgccatat
      841 tgcccacttt ttccgccttt ttcatcgcat cgtcgccgaa attcaattcg gcaaccgttt
      901 gcttcaacac atcgcgaaaa cgttgcaatc ctacctcgcc ttgaatcacg gtaaaacgga
      961 aggggcgcat attgccgtga tcgggaactt gggttgccgc ctgaaatatt tgttccaact
     1021 ccgccgcatc gggggcgggg tgcttcagct ttttggaaga tcggcggttc gtcaataatt
     1081 ttaaagcatc catatcgtta tactccggtc atcggtcggg tgttcggaca catatgccgt
     1141 ccgaaggctt cagacggcat atccggcatc agcgcggacg gcggcaggct gccaatatat
     1201 ccatttcctt ccgataggtt tggctattgg aaatgtccat cagccccaat accgtgctga
     1261 aatagtggtc gtgcgaatat tcgttttccg ccgctttttg tttgaggcat tggaaatcta
     1321 tgcccccgtg ttggcggaag gctttggaaa accacataac catcgggata tgcgtctgcc
     1381 cggaaggcgc gatggcgtaa ggcgcggcgt gcaggtacat cccgttttcg cccaaacttt
     1441 cgccgtggtc ggaaacataa tgcaccacgc tttccaaatc gtcgcggttt tcaagtttgc
     1501 ggataacctt gtcgataaac tggtccacat acaaaaccgt attgtcgtaa gtgttgacca
     1561 gcgtggcgcg ggtgcatttg ttgatttcgt tggtgtcgca ggtcggcgtg aatttgcgtt
     1621 cggcttcggt atagcgttcg taatacgtcg gcccgtggct gccgatggta tgcaggatta
     1681 aaaccgcgtc tttatcgttt ttgttgagga cttcgtcgaa cttagtcagc aggatattgt
     1741 cgaggcactc gccgttgcgg cagtattcgg gcaggttgag cgaggtaacg tcggtattcg
     1801 gcactttgcc gcacacgccc ttgcagccgg aatcgttttc caaccaggta acttccacgc
     1861 cggcgcgctg cacgatgtcc agcaggttgt cttggtgttc ggctttgatt tcgtcataat
     1921 ccgtgcggtc gaaggttgag aacatacacg gcagggagtg cgcggtcgat gtaccgcagc
     1981 ttctgacctg cgggaaattg acaatttcat cgccgcgcgc ggcaagcagc ggcgtagttt
     2041 ggcggctgta accgttcaaa ccccagttgg cggcacgcgt ggtctcgccc acgaccagca
     2101 ccacgaaacg gcgcaggctg ccggccggcc ggttttgcac gaccgccata tccaattgcg
     2161 tataaggaat attggaacgc ttccaatctt tgtatttcga cacgcccgcg ccgatgaaat
     2221 tagacggcac aatcagatgg gttactgatt tattgttgcg gaaaaacgag gcgtaatcct
     2281 gatattgcaa cattgcgatg cccaacgcgc acaaaaagga aacggcggca agcacaaggc
     2341 gcgtcaaaag ctccttatac caaacgcggt atttaacctt gacggcgata tacgccagcg
     2401 cgggcaatac gcccaaacat acaatccaca gcacatagcc cggcgtaatc aggcgcgcgc
     2461 tttcggcagc cgtagtttgc aagacattat tcaacatcga cttgttgaaa tagatattga
     2521 aaaatatttc ttggtaagac accgccgcac tgataaccaa tatcaacgga atcaatacct
     2581 tatgcacgaa aggcagggca atgacgtgaa aaacgaaatt acttaaaaaa aacagcacca
     2641 ccggcatcgt atagaggaag atatccgccc cggtgccgtt aaaaggatga agctcgacaa
     2701 ctttggcaaa aaaggcgtaa ttcaatacca gcgaggaata cagggaaagg aaggcaatca
     2761 gcgcggaaga gccgagcttc ggcctcaggt tcggttttat catttggaat gtgtcggata
     2821 agggttggaa aaggcatccg gcatttggaa tccggattat tgaaaaagat tcttaattat
     2881 aaggcaacgg agcaaagcag ggcaagaaaa cggaaggcga cgcaggctgt cggatacaca
     2941 tctcggcaca aacagcatca cgcccggatt gaaaaggacg gatagccgcc ggcagcacaa
     3001 tcttaccacc tccaaaacgc cgccggagaa cgccgaaaca gccgatgccg tctgaagccg
     3061 cttcagacaa catcgggaca tcaaccgtaa cgccgttgga aatcgcgcat aaaatctgcc
     3121 aaagcccgca cgccttcaag cggcatcgca ttataaatgc tggcacgcat accgccgacg
     3181 gttttatagc ccttaagcag gcacaagccc tgcaattcgg cttccagcac aaaacggcgg
     3241 tcaagctcct catcctccgt ttggaacacg acattcattt tagaacgcgc attcggacgg
     3301 atacggttga tatagaaacc gccgctgcca tctatcgtct catacaaggt ttgcgccttc
     3361 agccgattga ccgcttcaat ttttttcaca ccgccctgcg cctgcagcca gcggaacacc
     3421 agccccgaca tataaatcgc gtaagttgac ggcgtgttgt acataccgtc gcggttgatg
     3481 tgcgaacggt agttgaacac atcgggaata tcgttcggac aacgctcgag caaatcctcg
     3541 cggataatga ccacggtcgc accggccggg ccgatgtttt tctgtgcgcc tgcgtaaatc
     3601 agtccgtagt cggcaacatc aaactcgcgc gacaaaatct cgctggacat atcgcacacc
     3661 agcggcggca tgccttctga aaggcgcggc acttcacggt attgcagccc gttgaccgtt
     3721 tcattgacgg caaaatggac aaacgccgaa tcgggtgcaa catcccacgt ttccacaggc
     3781 ggcaggtcga gatagtcgaa ctgctcgccg ccatgcgccg ccaaacggat ttccgtatcg
     3841 gtcaaacggc tcatctgttc ataagcgata cggctccagt tgcccgttac caccgcgtcg
     3901 gcagtgcgga aaccgtgtgc cagattcatg gctgccatat taaattgggt tgttgctccg
     3961 ccctgcagaa acaatatctt atagttgtca ggcactttca aaagctgcct caaatcctgt
     4021 tccgcatgat gcaggatgct caaaaacatt tccgaacggt ggctcatcgc catcacggga
     4081 aaacccgtac cgttgtagtc caacatttcc tgccgcgccg tttccaacac ggcttcgggc
     4141 aatacggcag ggccggcgga aaaattgtaa atcggataaa gagacatgat gcagccttga
     4201 ttctgaacaa taacccgcct gattttaggc ttgcagcagg ctttgtgcaa ggatgggaaa
     4261 tacctgtcct ccgccccgct tcatgccgcc cgaacacgga aaacaacatc aatatattga
     4321 tttacaaata taaaaatcat gctagcgaca aatagataca tttgttttgt caacaatatt
     4381 cacgatttcc cattacaaac ctcccttaca cccgcttttt tccgtcccaa aaacacaaaa
     4441 taaatcaaca ctttcatttc tccgcaaaag cggttataat cacgccgatt tttcaacttt
     4501 gacgaaaaat ggccacgggg ccattttttt attatgtaca tagggagcag catggatatc
     4561 caaaccatcc tcgaaaaaac cctgcccggc ctgggctacg aactggtcga tttcgaactg
     4621 accgcgcaag gaacattgcg cgtgttcatc gacaaagaag gcggcattac cgtcgaagac
     4681 tgcgcaaccg tcagcaacca cttgagccgc gtatttatgg ttgaagacat cgactacaaa
     4741 aacctggaaa tttccagccc cggactcgac cgccccttga aaaaagccgc cgacttcgtg
     4801 cgctttgccg gtcagaatgc caaaatcaaa acccgcctgc cgatagacgg tcaaaaaaac
     4861 tttatcggta aaatcgaagg ctgcgaaaac gacactgtta ccgtttcctt cgacggcaaa
     4921 accgtacaaa tcgaattggg caacatcgac aaagcccgcc tgcgccccga attcaaattc
     4981 taaaacacaa caatattgga gatgttcaaa atgagtcgtg aaatgttaca gctggcagaa
     5041 gcactggcaa gcgaaaaaaa cgttgatgcc gaagtcgtct tccaagcact ggaattcgcc
     5101 ctgtctaccg ccgccaagaa aaaggcagac cgcgaacaca tggatgtccg cgtacaaatc
     5161 aaccgcgaca ccggcgaata ccaaaccttc cgccgctggc tgattgtcgc cgatgaagac
     5221 tatacctatc ccgatgtcga aaaaaccatc gaggaaatcc aagaggaaat tcccggcact
     5281 accatccaaa tcggcgaata ctacgaagag cagctgccca acgaaggctt cggccgccaa
     5341 gccgcgcaaa ccgccaaaca aatcatcctg caacgcatcc gcgatgccga gcgcgagcag
     5401 aatctgaacg agtttctcgc cgtcaaagaa gacatcgtgt ccggcacggt caaacgcgtc
     5461 gaacgccacg gcatcatcgt cgaagtcgtt gccggcaaac tggacgcgct gattccgcgc
     5521 gaccaaatga ttccgcgcga aaacttccgc agcggcgacc gcatccgcgc cctcttcctg
     5581 cgcgtcgaag aaatcggcaa caccggccgc aaacaagtca tcctgagccg tacttccggc
     5641 gatttcctcg tcaaactgta cgccaatgaa gtacctgaaa ttgcagacgg catgcttgaa
     5701 atccgcgctg tcgcccgcga cccgggccag cgtgccaaag tcgccgtcaa agccaacgac
     5761 cagcgcatcg atccgcaagg cacctgtatc ggcgttcgcg gttcgcgtgt caatgccgtc
     5821 agcaacgaat tgtccggcga gcgcatcgac gttgtccttt ggtcgcccga gcctgcccaa
     5881 ttcgtcatga gcgcgctctc acccgccgaa gtcagccgca tcgtcatcga cgaagacaaa
     5941 cacgccgtcg atgtcatcgt tgccgaagac cagctcgcgc tcgccatcgg acgcggcggt
     6001 caaaacgtgc gccttgcttc cgacctgacc ggctggcagc tcaacatcat gacttccgcc
     6061 gaggcagacg aacgcaatgc ggcagaagat gccgccatcc gccgcctgtt tatggatcac
     6121 ttgaacgtgg acgaagaaac cgccgacgta ctggttcagg aaggttttgc aaccttggaa
     6181 gaagtcgcct atgttcctgc cgccgaactg cttgccattg aaggatttga cgaagaaatc
     6241 gtcgatatgc tccgcaaccg cgcccgcgat gccatcctga ccatggcgat tgccgccgaa
     6301 gaaaaactgg gcgaagtatc cgacgatatg cgcaacctcg aaggcgtaga tgccgatatg
     6361 ctccgcagcc ttgccgaagc aggcattacc acccgcgacg acttggcaga gcttgccgtg
     6421 gacgaactga ttgaaattac cggtgtaaac gaagaaaccg caaaagccgt catcctgacc
     6481 gctcgcgaac actggtttac cgaagacaaa taaaaggggg tacagatgag taacacaacc
     6541 gtagaacaat ttgccgccga gctgaaacgc cccgtcgaag acctgttgaa acagttgaaa
     6601 gaagccggcg tcagcaaaaa cagcggcagc gattccctga cgctggacga caaacagctt
     6661 ctgaacgcct acctgaccaa gaaaaacggc agcaacggcg gcaccatcag catccgccgc
     6721 accaaaaccg aagtcagcac cgttgacggc gtaaaagtcg aaacacgcaa acgcggacgc
     6781 actgtcaaca ttccttctgc cgaagaattg gcagcacagg taaaagccgc ccaaacccaa
     6841 gccgcacctg tccggccgga gcagacggca gaagacacgg caaaagcccg agccgaagct
     6901 gccgcacgtg cagaagcccg tgccaaggca gaagcggaag cggcaaaact gaaagcggca
     6961 aaagcaggca acaaagccaa acctgccgcg cagaaaccca ccgaagcaaa agccgaaacc
     7021 gcacctgttg cggcggaaac caaacccgcc gaagaaagca aagcggaaaa agcccaagcc
     7081 gacaaaatgc cgtctaaaaa acccgccgag cccaaagaaa aagccgccaa gccgaaacac
     7141 gagcgaaacg gcaaaggcaa agatgccaaa aaaccagcga aacctgccgc acctgccgtg
     7201 ccgcaacccg tggtcagcgc agaagaacag gcgcaacgcg atgaagaagc acgccgtgcc
     7261 gccgcacttc gcgcccacca ggaagccctg ttgaaagaga aacaggaacg ccaggcacgc
     7321 cgcgaagcca tgaaacaaca ggcagaacaa caggcaaaaa ccgcacagga agccaaaacc
     7381 ggcagacagc gtcccgccaa acctgccgag aaaccgcagg cagccgcgcc ggccgtcgaa
     7441 aacaagcctg tcaatccggc aaaagcgaaa aaagaagacc gccgcaaccg cgatgacgaa
     7501 ggtcaaggcc gcaacgccaa aggcaaaggc ggaaaaggcg ggcgcgaccg caacaatgca
     7561 cgcaacggcg acgacgagcg cgtacgcggc agcaaaaaag gcaaaaaact caaactcgag
     7621 ccgaatcaac acgccttcca agcaccgacc gaacccgtcg ttcatgaagt tttggttccc
     7681 gaaaccatta ccgttgccga tttggcgcac aaaatggcgg tcaaaggcgt ggaagtggtc
     7741 aaagccctga tgaagatggg catgatggtt accatcaacc aatccatcga ccaagacacc
     7801 gccctgattg tggtggaaga actcggccac atcggcaaac ctgccgcagc cgacgaccct
     7861 gaagcattct tggacgaggg cgcggaagcg gtggaagccg aagcattgcc gcgtccgccc
     7921 gtcgttaccg tcatgggcca cgtcgaccac ggcaaaacct cgctgctgga ctacatccgc
     7981 cgtaccaaag tggtacaggg cgaagcaggc ggcattaccc aacacatcgg tgcataccac
     8041 gttgaaaccc cacgcggcgt gattaccttc ttggatactc cgggtcacga agcgtttact
     8101 gccatgcgtg cgcgcggtgc gaaagcaacc gatatcgtga ttcttgtggt cgccgccgac
     8161 gacggtgtga tgccgcaaac catcgaagcg attgcccacg ccaaagcggc aggcgtaccg
     8221 atggtggttg ccgtcaacaa aatcgataaa gaagccgcca acccagagcg tatccgccaa
     8281 gagctgaccg cacacgaagt cgtgcctgac gaatggggcg gcgatgtaca gtttatcgac
     8341 gtttccgcta aaaaaggcct gaacatcgat gcattgctcg aagcagtctt gctcgaagcc
     8401 gaagttttgg aactgactgc ccctgtcgat gcacctgcca aaggtatcat cgttgaggca
     8461 cgtttggaca aaggccgcgg cgcggttgcc acattgctgg ttcaaagcgg tacgctgaaa
     8521 aaaggcgata tgctgctggc cggtacggca ttcggcaaaa tccgcgcgat ggtcgatgaa
     8581 aacggcaaat ccattaccga agccggcccg tccatccccg tcgaaatcct cggcttgtcc
     8641 gacgtaccga atgcgggtga agacgcgatg gtattggcgg acgagaaaaa agcgcgcgaa
     8701 atcgccctct tccgccaagg caaataccgc gacgtgcgcc ttgccaaaca gcaggcggcg
     8761 aagctggaaa atatgttcaa caatatgggc gaaacccagg cccaatcttt gtcggtcatc
     8821 atcaaggcag acgttcaggg ttcttacgag gctttggcgg gcagcctgaa aaaactgtcc
     8881 accgacgagg tgaaagtgaa cgtattacac agcggcgtgg gcggcattac cgaatcagat
     8941 gtcaaccttg ccatcgcttc gggcgcgttc attatcggct ttaacgtgcg tgcagatgcc
     9001 tcttcgcgca aacttgccga aaatgaaaac gtggaaatcc gctactacaa catcatctac
     9061 gatgccatcg acgacgtgaa ggcggcgatg agcggcatgc tttctccgga agagaaagag
     9121 caagtaaccg gtacggtcga aatccgtcag gtcatctccg tttccaaggt cggcaacatt
     9181 gcaggctgta tggttaccga cggcgtggtc aaacgcgatt cccatgtccg cctcatccgc
     9241 aacaacgtgg tcatccacac gggcgaactg gcttcgttga aacgctataa agacgacgtc
     9301 aaagaagtcc gtatgggctt tgagtgcggc ctgatgctca aaggctacaa cgaaatcatg
     9361 gaaggcgacc aactggaatg cttcgacatc gtcgaagtgg cacgcaccct gtgattttga
     9421 tgcggaaaaa tgccgtctga agcgttcaga cggcatacgg aacgggttct gtatcataca
     9481 gaacccgttt tttatcgcaa atcggcttca gacagccctc ctgccttatc ccgatttgaa
     9541 tctgacttgc catacaaaca ggcttcagac ggcattatag tgaattaaat ttaaaccggt
     9601 acagcgttgg ctcgccttgc cgtactattt gtactgtctg cggctcgccg ccttgtcctg
     9661 atttaaattt aattcactat attttcccga caggcggatt tgaagtttct ccacatatcc
     9721 ggcgcgttcg gcgcggctgg tttcggggcg gtgcgtattg agcgacgacc atttccaatg
     9781 actgcgggct ttgttgagtt cgggcgggag tctggcggcg tcccacgggg ctttgcggct
     9841 gtgcagctcg atatccgact gtgccgcgtg tccgcgcgtt tgcaggacgt ggagcaaatc
     9901 gagggcgcgg gcggcgagca gggtcaaggt ttcagggtcg gtgtgcaagg tttggcggcc
     9961 ggagagtttg tcggaaatgg tgcgggtatt gggcaggatg ccgcccaaaa agccgccgat
    10021 tgccgtaccc aatccgagcg agccgccgag tgtggcgatg tccagcccca agccgatgag
    10081 cgcgccggtt gccgcgcccg tgccggtgcg gatgccgtat tgtttgagca attcgctgtc
    10141 gaacgggtct tggcggaagg cttgcggcat ccagtcgccg ccgtcgattt cgctgtggta
    10201 gaaacggtag agggcaaaca gccgctgctg catctgccgt tcgagttggc gtatttccgc
    10261 ctgcatggtt tgtagcacgg tggcggtatc ctcgttttcg tccacttcct gcctgaaggc
    10321 ggcggcatca attaaaaagt cggcgatttc gcggcgcgct tcgccgtcca gccgctgcca
    10381 ttcgcgccgg cgcatggctg tcaggcggtc aagtgtgctg cgttcgggca acatggtggc
    10441 gaggttttcc cacaggcgta gttcgccttc aaaatcaaag gcgacggtgt cgaaccctgc
    10501 gaaaacgtgc aggtttctcc tcgccagcat ggttgtccac gattcgggag gctgtccgcc
    10561 ggtaaagttg aacacgggca taaccggttt ggcacaccat gaaaggatgg tcaactcgtc
    10621 cctgtatttg tcgaggacgg gttcgcgcgc gtcgatgacg tacattgcca tatcgctttg
    10681 caagacttgc cgtaagactt tggcttcctg attgaaatca tggtgcgcgc cgtggctgcc
    10741 gagaaactgt tgcagccgtt cgatgccgtc tgaacgattg tccgtatggt tttccagcca
    10801 ttccagcacg ccgcctgcgt cttcgagtcc gggcgtgtcg tacaggaaaa ccagcgtgtc
    10861 tgcgccgtcg ctgatggcgg cttcttcgac atgacgcgtg gtcgatgggg cgtttctgac
    10921 ttcgccgaaa ccgctgtcgc gcaacagggt acgaaggagc gaggttttgc cggtgttggt
    10981 atgcccgacg acggcgaggg aaaggggttg tttgttcatg atgtttttga agaatggatt
    11041 ttcagacggt cttttttcag aatggcggct taacagaaca tttcaagtga gtttattggt
    11101 ctttcaaacg cccttcctgc gccgccctgt caggctcaag ccacgccgcg ccgcatttgg
    11161 ccagcgcgtt acgccaatgt tccagctttt ccgaaaggtc gtctgaaagc ccctgttccg
    11221 ccaaaagctg caccaccgcg ccgccctgcg ccgcttccga aagtcggacg atctgccgca
    11281 acacgccgcg gtcgggcaca gtttgggcgc gcacgccgat aagcagttgc gccggtttct
    11341 gcttcagctc tgtctccagc gcggcaacct gttcccgatt ggcggcaacg cccttatcca
    11401 gccattcctg cgccagcctg ccctcgaacc attcgccgtc ctgccattcg gtctccagca
    11461 tgaccgccca tttcggcgca tcgttcaaga cgattttcgg cgaaacggcg gacacggttt
    11521 cccgacgcgt atccgcatcg gtgattttgt tctgccagcg gcggatgacc gcctgataat
    11581 agggtttttc caaatccaat ccgttttcgc ttgttttcaa aaggattttg cataccgccc
    11641 aagccaagag gcgcggcagg atgccgtagc aggcgatact gccgaccagc agccccgacc
    11701 aagcccgcgc atcggcaata ttgccgttca gacgaccttc gatgaccgcc cgcgcatcag
    11761 gcacgggaaa acccagtttc gcaggcagcc atgccaacat ttccaccgcg cgtaccgaag
    11821 cggcattgct caacagcgtg ctttcccagt tgaacgtata ttgccgcacc aaaagcagca
    11881 acaataccga caccagcatt ccgagcagcg tgcagagcca caggctgtgc gacgttgcgc
    11941 ctattttcca acgtaccgaa ggttgccgcc actcgtccgc atacagccgc aacaccgcct
    12001 gatttacagg gtctttgccc cgaaaccacg tcgccggact gctgaaaaaa cgccccactt
    12061 tcacgcgcag gaacaacatt gccaaccata ctgccagcat cagcgtattc atgcccaaca
    12121 cgcccgccaa aaccaaaaag aaattcagac cctgattgtc cattagaaga taagtacctg
    12181 aaaaaccggt aaaaaacgca aacgtcgccg ccgccaccca caaccagaac gaccccgcac
    12241 gcacacgttc caacgtctcc cgcagcatac ggttcctgtc aatcatctcc gcccgacgga
    12301 tgattttttc ctccgtactg ccgtccacgc ggcgcaaagc ctccgtcgcc tgcacgggat
    12361 cgccgctgaa aataaaaccg ccttcgtcca aaatacggat cagctcaacc agttttcggg
    12421 atggattcaa cataaaatgc cgtctgaaaa taaaaaacag attttaacac acgcattttc
    12481 aagaatattc acagtgtagg caaagagtaa atctcacaca gaagcaaaag tatcggcgta
    12541 aactgactgc ctctactttc ccgaaagatt gtgcgatgta tacaggcgaa cgcttcaata
    12601 cttacagcca tttgagcggt ttgattctgg cggcggcagg tttggcgctg atgctgctga
    12661 aaaccatagg acacggggac ggctaccgta tcttcagcgt atcggtttac ggcatcagcc
    12721 ttcttctgct ctatttgagt tcctcgctgt accacggaat tgcagccgga aaactgaaaa
    12781 gcattttgaa aaaaaccgac cactgcatga tttatgtcct gattgccgga agctacacac
    12841 cgtttgcact ggtttctttg agaaacgggc cgggctggac ggtattttca ctgtcctggc
    12901 tgctggcggc tgcaggaatc gcacaagaac tcaccatcgg acggaaaagc gaaaaacgac
    12961 tgctgtctat tgcgatttat gtcgtcatgg gttggatggt cttggcggta atgaaatccc
    13021 tgacagcctc actcccgccg gcaggactgg cttggctggc ggcaggcggt atgctgtaca
    13081 gcgtcggcat ttactggttt gtaaacgatg aaaaaatccg acacgggcac ggaatctggc
    13141 atctgttcgt attgggcggc agcatcaccc aatttgtcag cgtgtacggt tacgtaatct
    13201 gaatgccgtc tgaaaagcaa aacctcccga tcctgaagat tgggaggttt tctgtttgcc
    13261 ggacatcagc ccttgtcgtg gaactcgtga aattcatact gataggacaa atcccgaccc
    13321 gcttttttct gtgccaaata atcatcataa atggcacgga tttccttacg caacaaaaac
    13381 agggctatca agttcgggat aaccataaag ccgttgaaca tatcggacag gctccaaacc
    13441 aaatcgactt tgccgagcgt acccaaaacg atggcaagca gaaccaatgc gcgatagatg
    13501 cccaagtgtc ttcccctgaa aagaaaacgg atgttggact caccgaaata ataccagccg
    13561 ataatggtcg tcaaggcgaa aaaggtcaga cacacggcaa gcaattgcga accgaagccc
    13621 ggaaatgcct tgttaaaggc aaattgagta accgccgcgc cctgttcgcc cgaaaggttg
    13681 gcatcggtca gcaggataat cagcgcagtt gccgtacata ccaaaatcgt atcgataaat
    13741 acaccgacaa atgccgccat accttgctgc acaggatgct tcacatccgc agtcgcgtgg
    13801 gcgtgcgggg tagaacccat ccccgcttcg ttggaaaaca gaccgcgcgc cacgccgaaa
    13861 cggacggctt cgcgcatacc gatacccgcc gcaccgccca aaacggcttc gggattgaag
    13921 gcggcggtaa agatgtggtt gaacatcggc acaatatggt cggaaaattc aaacaggata
    13981 acgacggcgc acaaaatata aacaaccgcc ataaacggca cgacaaattg ggcgatattg
    14041 gcaatacggt tcacgccgcc aatcacaacc atgcccgcaa ggacggcaag cacaataccg
    14101 actgccaaag aaggcacatc aaatgcaatg gtaacggcag aagcaatgga gtttgcctgt
    14161 gtcgcattac cgataaagcc caatgcgata atcaacgcaa tggaaaagaa accggacaaa
    14221 aaacgcgccg cgcccctgcc gattttcgga gtcagaccgt gggtgatgta gaacgccggc
    14281 ccgccgatgt atttgccgtg gctgacgacg cggtatttct gcgccagcag tgcctccgca
    14341 aaaatcgtgg acatccccaa aacggcagaa acccacatcc aaaaaatcgc gcccggcccg
    14401 cctgcggtga tggcggtcgc cacgccggcg acgttgcccg taccgatttg cgcagatatg
    14461 gcaaccgcca acgcctgaaa ctgcgataaa gacttgtcgt ctttatcgcc tttggcaaac
    14521 aagccgccga atacggattt gaatcccgcg cccagcttgg taatctgcgg cgcaccaaga
    14581 tacagcgtaa aaaacaggcc gatacccaaa agcgcgtaaa tcagcaggta gtcccaaagg
    14641 aaccgattga ctgtacccac cagaacagac aatatatttt ccataaaata aaccttatct
    14701 tacaattaaa atgactgcct tccaaaagac attccaataa ggaaacacgg cgagcagacc
    14761 gtatttgccg caacagatgc cttaaattgt caacaatcgg ggagaagctg cgccatcata
    14821 ccgtaaaata tcgttaatta aaacatttct ttatttttaa gcggaaagcg gaggaaatcg
    14881 ctttcagacg gcatcgacaa cggcacggca taaaacagga tattttgggt acttgcaact
    14941 tatgttaaaa tgccgaccgt aaaaaatctg acaaaaacag attaattatt tgaaataaga
    15001 aaggaaattt atggcaggcc atagcaagtg ggcgaatatc cagcataaaa aagcccgtca
    15061 ggatgccaaa cgcggcaaaa tcttcacccg tttaatcaaa gaaatcaccg ttgcggcgcg
    15121 tatgggcggc ggcgaccccg gttcaaatcc gcgcctgcgc ctggctttgg aaaaagcagc
    15181 cgaaaacaat atgcccaaag acaatgtgca acgcgccatc gacaaaggta cgggcaactt
    15241 ggaaggcgtg gaatacatcg aattgcgcta cgaaggctac ggcatcggcg gcgcggcttt
    15301 gatggtggac tgcctgaccg acaacaaaac ccgcaccgtt gccgacgtgc gccacgcgtt
    15361 taccaaaaac ggcggtaact tgggtactga cggctgcgtg gcgttcaact tcgtgcatca
    15421 gggctatttg gtattcgaac ccggcgttga cgaagacgcg ctgatggaag cggctttgga
    15481 agccggtgcg gaagacgtgg ttaccaacga cgacggttcc atcgaagtca ttaccgcgcc
    15541 aaacgattgg gcgggcgtaa aatccgcttt ggaggcggca ggttacaaat ctgttgacgg
    15601 cgacgttacg atgcgcgccc aaaacgaaac cgaactctcc ggcgacgatg ccgtcaaaat
    15661 gcaaaaactg attgacgcgc tggaagactt ggacgacgtg caagacgttt acacttccgc
    15721 cgtattgaat ctggactgat acgcagcaca gcagacatac aataaaatgc cgtctgaacc
    15781 tttcagatgg cattgattta tttagccctt gcccacgccc accaaaccgt caggacaacc
    15841 gccagaaaat acgccgcgct ccaaacaggc aaagcaaccc ctaacagata atccggttcg
    15901 gcacaatttc cgaacccgcg cacgacaggc tcgaaccaat caaacaaaga ccagcccttc
    15961 aagcggaacg tccacggcgc accgcacgaa ggagccgtac ccggcggcag cgactgcaac
    16021 cacaactgat atgccgcaac agaaataccc gtaacggccg gaatgctgat aaagacagca
    16081 ccgaacaaac cgcccgccct tcttcttggt ctgcacatca ggacaattgc cgtacacaat
    16141 gcggttgcca aaacgcataa ccgctgactg atacacaaaa cgcaaggctc catacccaaa
    16201 acatactgtg ccgccaaaga accggcaaat gcacagaccg aaacggcaaa cagcagccaa
    16261 acggcttttc taaataacgg ggtcattttc tcaacacacc aatcaaaata ccgatatgcc
    16321 gattttgctg gatatatccc gagatggcaa gggacgaatc gacaccggct caggtgcgga
    16381 cgatttcggt tttccccgat atgcacaaaa cctgacaagg gacaaaacgg
//
LOCUS       DAWHXZ010000023        15071 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000023 DAWHXZ010000000
VERSION     DAWHXZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.023"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            82..891
                     /gene="aroE"
                     /locus_tag="WIE35_000478"
     CDS             82..891
                     /gene="aroE"
                     /locus_tag="WIE35_000478"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ4153442.1"
                     /translation="MHALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIFLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSREIFRNCRLAYDMVYGDA
                     AQAFLNFAQSNGAAEVSDGLGMLVGQAAASYHIWRGFTPDIRPIIEYMKAL"
     gene            894..1595
                     /gene="mtgA"
                     /locus_tag="WIE35_000479"
     CDS             894..1595
                     /gene="mtgA"
                     /locus_tag="WIE35_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ4153443.1"
                     /translation="MFRIIKWLIALPVGIFIFFNAYVYGNIITYRAVAPHQTAFMSMR
                     MKQFEQEGRDVALDYRWMPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTACVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(1920..1995)
                     /locus_tag="WIE35_000480"
     tRNA            complement(1920..1995)
                     /locus_tag="WIE35_000480"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1960..1962),aa:Phe,seq:gaa)
     gene            complement(2186..2920)
                     /gene="lptB"
                     /locus_tag="WIE35_000481"
     CDS             complement(2186..2920)
                     /gene="lptB"
                     /locus_tag="WIE35_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ4153444.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(2994..3524)
                     /gene="lptA"
                     /locus_tag="WIE35_000482"
     CDS             complement(2994..3524)
                     /gene="lptA"
                     /locus_tag="WIE35_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ4153445.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(3505..4086)
                     /gene="lptC"
                     /locus_tag="WIE35_000483"
     CDS             complement(3505..4086)
                     /gene="lptC"
                     /locus_tag="WIE35_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ4153446.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(4083..4619)
                     /locus_tag="WIE35_000484"
     CDS             complement(4083..4619)
                     /locus_tag="WIE35_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ4153447.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(4837..5811)
                     /locus_tag="WIE35_000485"
     CDS             complement(4837..5811)
                     /locus_tag="WIE35_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ4153448.1"
                     /translation="MAGNEKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            5908..6963
                     /gene="tal"
                     /locus_tag="WIE35_000486"
     CDS             5908..6963
                     /gene="tal"
                     /locus_tag="WIE35_000486"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ4153449.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDSALDTTLPDRLK
                     GKIAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLV"
     gene            7117..7407
                     /locus_tag="WIE35_000487"
     CDS             7117..7407
                     /locus_tag="WIE35_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153450.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            7424..8311
                     /gene="gluQRS"
                     /locus_tag="WIE35_000488"
     CDS             7424..8311
                     /gene="gluQRS"
                     /locus_tag="WIE35_000488"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ4153451.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARAHGGRWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWYGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAAAVHGADGFVYNGRCRNPQQRPALQGKQPAWRIRVPDRDIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLNLPEAPEADRPAELLD
                     WAVAHWDMDKVPKHAITTP"
     gene            complement(8381..9391)
                     /gene="dusA"
                     /locus_tag="WIE35_000489"
     CDS             complement(8381..9391)
                     /gene="dusA"
                     /locus_tag="WIE35_000489"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ4153452.1"
                     /translation="MNDNTHTLPPRRLSVAPMLDWTDRHYRYMARQITRNAWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTANPIGYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANT
                     RE"
     gene            complement(9422..9913)
                     /locus_tag="WIE35_000490"
     CDS             complement(9422..9913)
                     /locus_tag="WIE35_000490"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ4153453.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHSDTEK
                     DAHYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(9987..10745)
                     /locus_tag="WIE35_000491"
     CDS             complement(9987..10745)
                     /locus_tag="WIE35_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ4153454.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(10816..11682)
                     /locus_tag="WIE35_000492"
     CDS             complement(10816..11682)
                     /locus_tag="WIE35_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ4153455.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(11731..12009)
                     /locus_tag="WIE35_000493"
     CDS             complement(11731..12009)
                     /locus_tag="WIE35_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ4153456.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(12009..12299)
                     /locus_tag="WIE35_000494"
     CDS             complement(12009..12299)
                     /locus_tag="WIE35_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ4153457.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(12302..12832)
                     /locus_tag="WIE35_000495"
     CDS             complement(12302..12832)
                     /locus_tag="WIE35_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ4153458.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVIFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            13038..>15071
                     /locus_tag="WIE35_000496"
     CDS             13038..>15071
                     /locus_tag="WIE35_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ4153459.1"
                     /translation="MPAGRLPRRCPMMTKFTDCTRSNRIQPPTHRGYILKNNRQIKLI
                     AASVAVAASFQAHAGLGGLNIQSNLDEPFSGSITVTGEEAKALLGGGSVTVSEKGLTA
                     KVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQVREYTAILDPVGYSPKTKSALSDGK
                     THRKTAPTAESQENQNAKALRKTDKKDSANAAVKPAYNGKTHTVRKGETVKQIAAAIR
                     PKHLTLEQVADALLKANPNVSAHGRLRAGSVLHIPNLNRIKAEQPKPQTAKPKAETAS
                     MPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAVRPEPVPAANTAASETAAESAPQEA
                     AASAIDTPTDETGNAVSEPVEQVSAEEETESGLFDGLFGGSYTLLLAGGGAALIALLL
                     LLRLAQSKRARRTEESVPEEEPDLDDAADDGIEITFAEVETPATPEPAPKNDVNDTLA
                     LDGESEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQNGILSGALTQDEETQKRADAD
                     WNAIESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTAENKPETVDTDFSDNLPSNNH
                     IGTEETASAKPASPSGLAGFLKASSPETILEKTVAEVQTPEELHDFLKVYETDAVAET
                     APETPDFNAAADDLSALLQPAEAPSVEENITETVAETPDFNATADDLSALLQPS"
BASE COUNT         3523 a         4340 c         3830 g         3378 t
ORIGIN      
        1 ctgaaaacag tttagacggc atcgttccca tcttcgcgcc gccgttataa aatgaccgcc
       61 ccgccttgat ggaatatgac tatgcacgcc ctcccccgct acgccgtttt tggcaacccc
      121 gtcgcccaca gcaagtcgcc gcagattcat caacaatttg cccttcagga aggcgttgac
      181 attgaatacg aacgcatttg cgccgacatc ggcggtttcg cgcaggcggt ttcgacattt
      241 tttgaaacag gcggttgcgg ggcaaacgtt accgtaccgt tcaagcagga agcgtttcat
      301 ctggcggacg agcattctga acgcgcattg gctgccggcg cggtcaacac gctgattttt
      361 ctgaaaaacg gaaaactgcg cggcgacaat accgacggta tcggtttggc caacgacatc
      421 acgcaggtca aaaacattgc catcgaaggc aaaaccatct tgcttttggg cgcgggcggc
      481 gcggtgcgcg gcgtgattcc tgttttgaaa gaacaccgtc ctgcccgtat cgtcattgcc
      541 aaccgcaccc acgccaaagc cgaagaattg gcgcggcttt tcggcattga agccgtcccg
      601 atggcggatg tgaacggcgg ttttgatatc atcatcaacg gcacgtccgg cggcttgagc
      661 ggtcagcttc ctgccgtcag ccgtgaaatt tttcgcaact gccgccttgc ctacgatatg
      721 gtttacggcg acgcggcgca ggcgtttttg aactttgccc aaagcaacgg cgcggccgaa
      781 gtttcagacg gcctgggcat gctggtcggt caggctgccg cctcctacca tatttggcgc
      841 gggtttacgc ctgatatccg ccccattatc gaatacatga aagccctgta accatgttcc
      901 gcatcatcaa atggctgatt gccctgcccg tcggcatctt tatctttttc aatgcctatg
      961 tgtacggcaa catcattacc taccgcgccg tcgcgcccca tcagactgcc tttatgtcga
     1021 tgcggatgaa gcagtttgaa caggaaggtc gcgatgtcgc actggattac cgctggatgc
     1081 cctacaaccg catctcggtc aacctgaaaa aagccctgat tgcttccgaa gatgcccgtt
     1141 tcgccgggca cggcggcttc gattggggcg gcattcaaaa cgccatcagg cgcaaccgga
     1201 acagcggcaa agtgaaggcg ggcggctcga ccatcagcca gcagcttgcc aaaaacctct
     1261 tcctcaacga aagccgcagc tatatccgca aaggcgaaga agcggcgatt accgcgatga
     1321 tggaagccgt taccgacaaa gacaggattt ttgaactgta tttaaactca atcgaatggc
     1381 actacggcgt tttcggcgcg gaagccgcgt cccggtattt ttatcaaata cccgccgcca
     1441 agctgaccaa acagcaggcg gcaaaactga cggcgtgcgt ccccgccccg ctctactacg
     1501 ccgaccatcc gaaaagcaaa cggctccgca acaaaaccaa tatcgtgctc agacgcatgg
     1561 gttcggcaga gttgcctgaa agcgacacgg actgattgtt tcagatatgg aaatgccgtc
     1621 tgaactgcgg ttcggacggc atatgttttc tggtttttat agtggattaa caaaaatcag
     1681 gacaaggcga cgaagccgca gacagtacaa atagcacgga atcgattcac ttggtgcttc
     1741 agcaccttag agaatcgttc tctttgagct aaggcgagcc aacgctgtac cggtttaaat
     1801 ttaatccact atacctgccc gacagccgca aagaccgtat catacatagc aggcattgcg
     1861 gtgcgggatt actgccgcaa acggcaggca aaaaaaataa ccgcttcaag gcggtttttt
     1921 ggtggccaga ggcggaatcg aaccgccgac acacggattt tcaatccgtt gctctaccaa
     1981 ctgagctatc tggcctttgt ctgtctcgtt aagagatgcg tattaaacca aattacggga
     2041 cgacatgcaa gcatatcggg caatattctt tgaaaacagg tttaagtcaa tgttttaaat
     2101 ttgaattaat ttatgtaaaa acaacataaa tccgtatttt tgccgcctgc ccgacgatat
     2161 taggtcgccc gtctgaaaaa tattttcaat atttgaagtt cttacccaga taaacagaac
     2221 gaacctgttc gtttccgacc aaatcatcag gttttcccga tgccagcacc gcaccgtctg
     2281 aaatgatgta ggcgcggtcg cagatgctga gggtttcgcg tacgttgtgg tcggtaatca
     2341 gtacgccgat accgcgcgac ttgagaaatc cgatgatttt ttggatgtcg atgacggcga
     2401 tgggatcaac accggcaaaa ggctcatcca gcaaaataaa atgcggtttc atggcgagta
     2461 cgcgggcgat ttcgacgcgc cgccgttcgc cgccggacag cgacggcgcg gggctgcggc
     2521 gcaagtgtcc gatattaagg tcggcgagca gtttttcgat ttccctgtcg atttgatttt
     2581 tatctttggt tctgatttcc aagatggcgc ggatgttttg ttcgacggtc attttgcgga
     2641 atatcgaggc ttcctgcggc aggtagccga caccgaggcg ggcgcgttcg tgtatgggca
     2701 ggtggcgcaa ttcttgtccg tctagggtta cgctgcctgc gtcggcggca atgagtccga
     2761 ctatcatgta gaagctggtg gttttacccg cgccgttggg cccgagcagg ccgacgactt
     2821 ctccgctttc gatttcgagg gagaagcttt taacgacttg gcgttttttg aaactttttt
     2881 gcaggttttg aacaacaagg cggctgacgt ttgcgctcat aaatatctct tcaatatctt
     2941 cggtcggcaa atgccgtccg aaccaggttt atgtttcaga cggcattttg ggattattcg
     3001 gatttttgcg tactcgaagg ctggataacg acgctgaccc tgccggattt ggaagcggat
     3061 tttgcgccgg attttgtgct gccgctgatg gtatagactt cggttttggt gttgtatgta
     3121 atcaccgcac cttcggcgac atcgccgccg cgctgtactt tggcattacc ggttaagact
     3181 acggtgctgc ctgcagatga ataagcaacg ttgtttgcct gtccgcgcac cgtgcctttg
     3241 ccgccgtcca atgtctggct gaagcggact ggcgaacctt ccgccctcac ggattcgccg
     3301 cctttgccgc cgcgtgtaac attgacgcgg gcggcggaaa tattgagcgt accctgtctg
     3361 atgacgacgt ttccgctgaa tgtggtgctt tggttggctt gatcgagcga accttggtcg
     3421 gcctcaatct gaataggctg cctgctgtcg ctttgaaggg caaaagcggg ggacgccgaa
     3481 aaaaatgcta ttaaaacaaa tagcttacat atcttttgta tcataaatcg tggctttcac
     3541 tttagatgag aagttcaaca tgcctgtttt gtggtcgtaa gtcatgccgc ccgcctgacc
     3601 gtgcgatgca ccatattgga aactgacagg cgtatcggtt tgggcatatt gagattcggt
     3661 atcgacgtgc agcttttcgg cttcaacttt acccgcctgc cgtttgccgt cggcggtttt
     3721 ggtcagcaca acgttgtttt taaaaagaac ctgtttgttt tcggtatggt aaacggcttc
     3781 atcgctgccg acttcgtaca acaacctgcc ttcttggaag aacacgagat gcggtgagtc
     3841 gaaatgaatg tcgctgcttt cgggaaactg tttcgcgccc ttcgaactca aatgttcttt
     3901 caagtatccc tgttcgtcaa accgcctgcc atccaatccg tccattgtgt attgcggttc
     3961 gtcgggattg agcctgactt cttcaatctc gacttcgctg atgcgtccca accatgccga
     4021 caggctgccc aaggcaaccg ccaatatcaa tgggaacgca attccgtacc gccatcttac
     4081 tttcatttga tgtactcgtt caaagccgcg cccaaagtcc cttgcgcctg cataatcagg
     4141 tcgcacactt cgcgcaccgc gcccgcaccg ccggggcgtt tggcaatata ggcggcgtgt
     4201 tgcagcgtaa accaatgcgc gtcggggacg gcaaccggca atccgcagcg taccataacg
     4261 ggcaaatcga ccacgtcgtc gccgacaaag gcacactcgg cttcttccac gccgacctga
     4321 gcgcgcagct cttcatacgc ggcgcgtttg tccgaaatgc ctttgaagta gtaatttatg
     4381 cccaactgtt tgacgcggat gccgacggag ggcgcgtccc gacctgtgat aatcgcagtc
     4441 tgcacgccgc ttgcctgaag cattttcaga ccgtgtccgt ccagtgtgtg aaacgatttg
     4501 atttcttcgc cgttatcgcg gataaagatg cgcccgtcgg tcaaaacgcc gtccacatcc
     4561 aggatcaaca gtttgatttt ggcggcgcgc gcctgtaatt cgggagaaat tgcctgcatg
     4621 ggatttcctt gttttcagac ggcatatctt accacaaatg ccgtctgaaa cggctgcaac
     4681 gccttatata gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaga
     4741 tagtacggaa ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta
     4801 aggcgaggca acgccgtact ggtttttgtt aatccactat acaatccgcg ccgccaaaag
     4861 gtcgtgcata ttcagcgcgc cgatcagcac gccatctgca tcggtaacca gaagcccgtt
     4921 cacatggttt gcctgcatga ctttcagggc ttcggtggcg agacgttcgg cggagatggt
     4981 tttaggatgc gtatgcatga cttcgtctat cgaaagaccg gtaaaattgt cgcattcttg
     5041 aaacaggcgg cgcaaatcgc cgtcggtgaa tacgcctttc agacggcctt gcccgtccgt
     5101 taccgccaac atgcccagcc ctttttcact catgctgacg atggcttctt tcaagggcgt
     5161 gccgagtcgg acggcaggca ggccgccgcc tttgtgcata atgtcggcaa cgcgcaaaag
     5221 taggcgtttg ccgaggctgc cggcagggtg gctcaaggcg aagtcgtcgg gcgtgaacgc
     5281 gcgggcgcgc agcaggacaa ccgccaacgc atcgcccaaa gccataacgg cggtggtgct
     5341 ggtggtcggg gcaagcccca gcgggcaggc ttctttggaa accgacgccg tgatgtggat
     5401 gtcggcatgg cgcgccatgg ttgaatcggg gcgggcggtg atgcagacaa gcgtgatatc
     5461 tttgcgtttg agcgcgggga tgatggcggc gatttcgtcg ctttcaccgg aattggaaat
     5521 cgcgacgacc acgtcgttgt ccacaatcat gcccaaatcg ccgtgtgccg cttccgcagg
     5581 gtggacgaaa aacgcgggcg tgccggtcga ggccatggtt gccgccattt tgcgcccgat
     5641 atgtcccgac ttgcccatgc ccgtgataac gaccctgcct ttgcagtgca acaacgcgtc
     5701 cgccgcaagg gcgaagtttt cgtccaaatc cgccgcaatt tcgcgcaagc cttccgcttc
     5761 ggtgtgcaac acttcgcgtg cccaatcaag atatttttcg tttcctgcca tcgccatccg
     5821 cctttgctgt aataaaacta cccaaaattt ccgaatcggg gtagcataag cctttgaaac
     5881 atacacatcc atccaaagga atcagaaatg actattttat cggacgttaa agcattagga
     5941 caacaaatct ggctggacaa cctttcccgc tcgctcgtgc aaagcggcga attggcgcaa
     6001 atgctgaaac aaggcgtgtg cggcgtaact tccaatcccg ccatctttca aaaagccttc
     6061 gccggcgatg cgctttacgc cgacgaggtt gccgccctca agcagcaaga cctgaccccc
     6121 aaacaacgct acgaaacgat ggcggttgcc gatgtgcgtg ccgcctgcgg cgtctgtctt
     6181 gccgaacacg aatccaccgg cggcaaaacc ggcttcgtca gcctcgaagt ttcgcccgaa
     6241 ttgtccaaag acgcgcaagg cacggttgaa gaagcgcgcc gcctctacgc cgccatcggg
     6301 tgcaaaaacg cgatgattaa agtgcctgcc accgacgcag gcatcgatgc gctcgaaacc
     6361 ctcgtttcag acggcatcag cgtaaacctc acgctgctgt tctcacgcgc ccaaaccctc
     6421 aaagcctacg ccgcctacgc gcgcggcatt gccaaacgct tggcagccgg acaaagcgtt
     6481 gcccatatcc acgtcgtcgc cagcttcttc atctcgcgtg tggacagtgc gctggacaca
     6541 acactgcccg accggctcaa aggcaaaatc gccatcgccc ttgccaaagc cgcctatcaa
     6601 gattgggaac aatatttcac cgcccccgaa tttgccgcac tggaagccca aggcgcaaac
     6661 cgcgtgcagc ttttatgggc atctaccggc gtgaaaaacc cagcctatcc cgacacgctc
     6721 tacgttgaca gcctgatcgg cgcgcacacc gtcaacaccg ttcccgatgc cacgctcaaa
     6781 gcctttatcg accacggcac agccaaagcc acgctgaccg aaggcgtaga agaagcacaa
     6841 gcgcagctcg ccgaaaccgc cgcgctcggc atcgatgtcg aaaccttggc aacgcgtttg
     6901 caggaagacg gtttgaaaca gtttgaagaa gcgtttgaaa aactgctcgc accgttggtt
     6961 taaacttttc atccccgatg ccgtctgaaa ggctttcaga cggcatcgcc cttttgaccg
     7021 gacacagtgc ggcatatcaa ggattcgccc taaaaatatt aatgggcaag aagttaatgc
     7081 acgacattac ccgccaacac aaaaggaaat agaaccatag caaaagcact cgaaatcatt
     7141 tcacccaatg aaatttattc agatctgatt tttaaggatc cggtacctcc ccatacaata
     7201 tatacagaat tgatgaaact ggttagtaga aatgcaagta atgagagaat cgcagataga
     7261 gccttcgatt ttttctcgtc cggccttatg gatggttcag ctaccgaaga gcaatataat
     7321 gccctatatg atttaacctt acttgaagaa cccgggatgg aactgaataa agatgagata
     7381 atggctttga ttaatagtct taaataaagg ggccgtaccg gatatgtaca caggacgttt
     7441 cgcccccagc ccgaccgggc tgctccacat cggctcgctg ctgaccgccg tcgcttccta
     7501 tgccgatgca cgcgcacacg gcggaaggtg gctggttcgc atggaagacc tcgatccgcc
     7561 gcgcgaaatg ccgggggcgg caagccatat cctgcacacg cttgaggcat tcggattcga
     7621 gtggtacgga gaagtcgcct atcagagccg ccgttacgcc ctgtatgaag aaaccctatg
     7681 ccgtctgaaa accgccggac tggtctatcc ctgccattgc agccgcaaag actggcaggc
     7741 tgcggcagtc cacggcgcag acggattcgt ctataacgga cgttgccgca atccgcagca
     7801 acgccctgca ctgcaaggca aacagcctgc gtggcgcatc cgcgtccccg accgcgacat
     7861 cggcttttca gacggcatcg tcggcggtta cgcccaaaac ctcgcccgcg acatcggcga
     7921 tttcgtcctg cttcgtgcag acggttactg ggcataccag ctcgccgtcg ttgccgacga
     7981 tgccgaacag ggcgttaccc acatcgtccg cggacaagac ctgcttgttt ctacgccgcg
     8041 ccaaatctat ttgcagcaat gtttgggcgt tccgacaccg caatatgccc acctgccgct
     8101 gctgaccaac gcacaagggc agaaatggtc gaaacagacg ctcgcacccg cattggattt
     8161 aaaccgccgc gaacaactcc tccgccaagt gttccgttac ctcaacctgc ccgaagcacc
     8221 ggaagccgac cgccctgccg aactgctcga ctgggcggtg gcacactggg atatggacaa
     8281 agtgccgaaa cacgccatta ccacccccta aacaccgccc gacaaacgca caaatgccgt
     8341 ctgaagcctt cagacggcat ttcacacata cagccccgcc ctattcgcgc gtatttgccc
     8401 gttcgacctc tttccacgct tcgagaatca ggctgccgtc gttgtctttc aagagcgttg
     8461 cgtcggaaag catacgccgc caagtccgcg cgcctttcaa gccgtgcatc agcccgaggc
     8521 tgtggcggac gatatggcgc aggattgtgc cgcgtccggc ttggatttgg gcttggctgt
     8581 atgtgtagag gcgttgcacc aaatcggcgt atccgatcgg gttcgcagtg tcgccgtaaa
     8641 acaatctgtc ccattcgcgc atcaccatcg ggttgtggta cgcctcgcgc ccgaccatca
     8701 cgccgtcaac gtgttgcagg tgtccggcga ttgcttcgtt ggtggtgatg ccgccgttga
     8761 tgatgatttc cagcccggga aactcctgct tgaggcggta aacgtaatcg tatttcaacg
     8821 ggggaacgtc gcggttttct ttgggggaaa gaccgtccag ccaagcgttg cgggcgtgga
     8881 cgatgaaggt tttgcaggcg gttttgtcgc gcaacgtgcc gacgaaatcg gcaacggttt
     8941 ggtattcggt ctgcctgtcc acaccaatgc ggtgtttgac ggtaacggga ataccgaccg
     9001 cgtcctgcat ggcgttgagg cagtcggcaa ccagcccgac ttcgttcatc agacacgcgc
     9061 cgaacgaacc tttctgcacg cgcggactgg ggcagccgca gttgaggttg acctcgttgt
     9121 agccgtatgc ttcggcggct ttggcggctt tcgccaaatc ggacggatcg ctgccgccca
     9181 gttgcagggc gacgggctgc tcgccttcgt tgaacatcaa aaagcggtct ttgtcgccgt
     9241 aaacaatcgc accggcattg accatttcgc tgtaaagcca agcatttcgg gtaatctggc
     9301 gcgccatata acggtagtgc ctgtccgtcc agtcgagcat cggggcgacg gaaaggcggc
     9361 gcgggggaag ggtgtgtgta ttgtcgttca ttgtcgatat ttcatcattg cagattcaat
     9421 ttcaatccgg aacaaccttc atttttgggg aaatccgcgt catgaaagag tccagcagta
     9481 tggttaccgg cccgtcgttg cagagcgaca cctgcatatg cgtgcggaaa cgccctgttt
     9541 cgacatgaat cccgtgtccg cgcaacagtt ccgccgttcg caggtaaagc tgctgcgcct
     9601 gttctgcagg tgcggcttgg gaaaacgaag gccgccgccc gcttgccgca tcggcataaa
     9661 gtgtaaactg cgacaccagc agcaccgcgc cgccgacatc tttcaaagac aggttcaact
     9721 tgcccgcttc gtcttcaaac acgcgcaaat gggcgatttt gtcggcgata tagtgcgcat
     9781 ctttttctgt gtcgctatgc gttacgccga gcagcacgac gaatccgccg tcgattttgc
     9841 cacaggtttc cgtgccgttt tcggacacga catccacctt tgcacctacc gttttctgta
     9901 tgaccgcacg cataagccgt cctccttacc tgaacgtgca atgccgtctg aaaaggcttc
     9961 agacggcatc ggtattggaa atgccattac ggtttgacac cgttttcttc caaaatggca
    10021 tcgattttca agcgggcttt ttcctgtctc aaaccttgtt ccaactggtt tctgaccaac
    10081 tcgaaaggct gcgcgtcggg gtttttcccg acctcgctga gtttgaacag ataatagcgt
    10141 tcgcccaatt tgaccggatc gcgggtaacg tcgccccgat tcatcgctgc aaactgcgaa
    10201 gccagcggct cgggaagctg ctgcgccata atgaaaccgt caaaagcctg ctcgtcgttc
    10261 ggataacgct tcatcagccc ttcaaaagac agccctttga gcaggagctg ctgcgcctga
    10321 cgcgcctcct cttcggttgc gaagctgacc tgctgcaatt tgatcatgcg gatttgccgc
    10381 tcataaaatt gacgcagtgc gctttcggaa accgtttccg aacgttccag aaaacggacg
    10441 tactcctcgg cataaaaaga cgcttcggcg attttaaagc ggttttggac atccttatcc
    10501 ttatccaaac cttccttcaa tgccctgttt ttcaaaactt ccaaagtttg cagccgacgg
    10561 acggcatcgt ttcggattgc ctgcccgtcc ggtttttggg actgctccgc atgccggtct
    10621 gcctgctgca tgatctgcgc caccagcgta tccaccaaag ccgggtcgat ttcgggtgct
    10681 ttggctgccg caaaacctgc caacattgca gcaataactg cggcagcggt ttttttctgt
    10741 ttcatggtat tccctgtcct gcgcggcggc aaaacgcctg tctgaacgtc ttgccgccgt
    10801 atcccgtttt cagaattatt ttgcaggttt gatgtttgcc ttgcccaaca gtgcaccgac
    10861 ggcacggtca atccgttccg cctgaaggtt gccggcaatc tgtcctttca tttcatcaaa
    10921 agaaggcact ttcacctcgc ggctgtcgtt gacataataa acgccgtaga aatcgccgtt
    10981 tttcagcggc gttgccgtaa attcgccttt tttcaagtcc ttaattgcct gataaagcgg
    11041 cggaacaccc tgttccaaat ctttcagcgg cacatatccg accggtgcgc cggtctgttt
    11101 ggtacggtcg ttgagggaat attgtttcaa gacggcatcg aaaccttttt tcgccttcaa
    11161 gtcggcaacc gctttttttg cattttcttc cttgtcggtc aggatttcgc ccaactggac
    11221 ttcctgcgta cctttgtaaa aaccgctgat attgtcatat gcggctttta cttcctgctc
    11281 ggaaaccggt tgggttttgg cgatatgcag cgcgtatgcc tcgccgttca agccatattt
    11341 taccgcctgc caaacggttt tgaaggacgg tttcttgtcg tcgcccgact ttttcgcttc
    11401 ggcacgcaat ttggcaagcg catttttaaa ctctgccgac cggtcgagtt tcaggcgttt
    11461 cacttcctgt gcgaccacgg tgttgaccac ttcgttttcc agcagggatt ggcgcagttg
    11521 cggcgtgtct tcggcacggc tgttttccgc acggaatgcg gcaacctgcg catcgatgac
    11581 ggaactgtcg attttctgac cgttgacggt tgccagcgtt tgggcaaaca ggctgccgga
    11641 acaggcaagc agtgcaacgg aagtcaggat ttttgctttc ataatcttct ctctttaaat
    11701 tttaaattga aatgttcggg cagacttcat tcagtccgcc gtatggaaat actcgtcagg
    11761 ggtagccgcc cggatgccga gcgcgtgaat catgccgcct gaaaacaaat ctttgagcag
    11821 cgatttgacc gttttctggc ggttcaggcg gcttacgcct tcaaaacggc cgctaacgac
    11881 caaaacggca taatgtccgc cgccggtatt gcccgcgtgt cctttgtgca gatggctctc
    11941 atcgccgatt tccaacacca gcggatcgag cgtctgcagg cgttcgcgga tcaaatcgac
    12001 ggccggcatt atttgaacac cgctttaaac ggcttgatca gcacttcgct gtacacgcct
    12061 gcatgaacat agggatcgtc ttccgcccac gcctctgccg catccaaaga ctcgaactgc
    12121 gccacaatca aactgcccga aacgcggttg gagtcctccg gcaaaggatt cgggcctgcc
    12181 gtcaacagcc ggccttccga cttcagcgtc tccagccgtt tgaggtgttc gggacgtgcc
    12241 gccatacgcg cttcatacac attctccccg tctgttgcca gcaacataaa atattccaca
    12301 gtcaatcctc ctttttcaga caggtactca gataaatacc ctgaataata aagaaaacaa
    12361 gcatcagtgc agtcgagccg aacattttat agttgaccca ttgcgactcg aaccgggtaa
    12421 acacaaacca gttggcgata cccataaaaa tcaggaaacc gacccacata tacgtcaatt
    12481 tcgcccatac ggcatccgga agctgaatct ccctgccgat actcgccttc aagccgtttt
    12541 taccggcgag gtggctgccc cacaggaaca gcgcgcccag ccaaaacaaa acgctcggct
    12601 tccacataat gaagcggctg tcgcccaaaa caatggttgc gccaccgaat atcacaatca
    12661 gcaccagtcc gacccactgc atcgtatcca gctttttata tttccaatac agaaaagccg
    12721 cctgaaccac accggcaacc aaggcgaccg ccgtagcggc aatcatattt ttggtaacgg
    12781 tataggtggc gaaaaacagg atgacggaca aaaggtcgct gacgaatttc atatccaaat
    12841 gagaaagcaa atcaaataaa cgctaatcat acaggcagaa ccctgttttt gcacctgcaa
    12901 aaccaacaaa ttttgttttg attgatgcaa atatgcacag atgtttttga aaaaagatgg
    12961 agatatgtca taatttgtaa aaacggcgaa tgattgccgt ttaaaatgtg gcggcggtcg
    13021 gtacattcac atattaaatg cccgccggcc gactgccccg ccgatgcccg atgatgacga
    13081 aatttacaga ctgtacgcgg tcaaaccgta ttcagccgcc aacccacagg ggatacatct
    13141 tgaaaaacaa cagacaaatc aaactgattg ccgcctccgt cgcagttgcc gcatcctttc
    13201 aggcacatgc tggactgggc ggactgaata tccagtccaa ccttgacgaa cccttttccg
    13261 gcagcattac cgtaaccggc gaagaagcca aagccctgct aggcggcggc agcgttaccg
    13321 tttccgaaaa aggtctgacc gccaaagtcc acaagttggg cgacaaagcc gtcattgccg
    13381 tttcttccga acaggcagtc cgcgatcccg tcctggtatt ccgcatcggc gcaggcgcac
    13441 aggtacgcga atacaccgcc atcctcgatc ctgtcggcta ctcgcccaaa accaaatctg
    13501 cactttcaga cggcaagaca caccgcaaaa ccgctccgac agcagagtcc caagaaaatc
    13561 aaaacgccaa agccctccgc aaaaccgata aaaaagacag cgcgaacgca gccgtcaaac
    13621 cggcgtacaa cggcaaaacc cataccgtcc gcaaaggcga aacggtcaaa cagattgccg
    13681 ccgccatccg cccgaaacac ctgacgctcg aacaggttgc cgatgcgctg ctgaaggcaa
    13741 acccaaatgt ttccgcacac ggcagactgc gtgcgggcag cgtgcttcac attccgaatc
    13801 tgaacaggat caaagcggaa caacccaaac cgcaaacggc gaaacccaaa gccgaaaccg
    13861 catccatgcc gtccgaaccg tccaaacagg caacggtaga gaaaccggtt gaaaaacctg
    13921 aagcaaaagt tgccgcgccc gaagcaaaag cggaaaaacc ggccgttcga cccgaacctg
    13981 tacccgctgc aaatactgcc gcatcggaaa ccgctgccga atccgccccc caagaagccg
    14041 ccgcttctgc catcgacacg ccgaccgacg aaaccggtaa cgccgtttcc gaacctgtcg
    14101 aacaggtttc tgccgaagaa gaaaccgaaa gcggactgtt tgacggtctg ttcggcggtt
    14161 cgtacacctt gctgcttgcc ggcggaggcg cggcattaat cgccctgctg ctgcttttgc
    14221 gccttgccca atccaaacgc gcgcgccgta ccgaagaatc cgtccctgag gaagagcctg
    14281 accttgacga cgcggcagac gacggcatag aaatcacctt tgccgaagtc gaaactccgg
    14341 caacgcccga acccgctccg aaaaacgatg taaacgacac acttgcctta gatggggaat
    14401 ctgaagaaga gttatcggca aaacaaacgt tcgatgtcga aaccgatacg ccttccaacc
    14461 gcatcgactt ggatttcgac agcctggcag ccgcgcaaaa cggcatttta tccggcgcac
    14521 ttacgcagga tgaagaaacc caaaaacgcg cggatgccga ttggaacgcc atcgaatcca
    14581 cagacagcgt gtacgagccc gagaccttca acccgtacaa ccctgtcgaa atcgtcatcg
    14641 acacgcccga accggaatct gtcgcccaaa ctgccgaaaa caaaccggaa accgtcgata
    14701 ccgatttctc cgacaacctg ccctcaaaca accatatcgg cacagaagaa acagcttccg
    14761 caaaacctgc ctcaccctcc ggactggcag gcttcctgaa ggcttcctcg cccgaaacca
    14821 tcttggaaaa aacagttgcc gaagtccaaa caccggaaga gttgcacgat ttcctgaaag
    14881 tgtacgaaac cgatgccgtc gcggaaactg cgcctgaaac gcccgatttc aacgccgccg
    14941 cagacgattt gtccgcattg cttcaacctg ccgaagcacc gtccgttgag gaaaatataa
    15001 cggaaaccgt tgccgaaaca cccgacttca acgccaccgc agacgatttg tccgcattac
    15061 ttcaaccttc t
//
LOCUS       DAWHXZ010000024        14905 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000024 DAWHXZ010000000
VERSION     DAWHXZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.024"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(90..3575)
                     /gene="smc"
                     /locus_tag="WIE35_000497"
     CDS             complement(90..3575)
                     /gene="smc"
                     /locus_tag="WIE35_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ4153460.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVRALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIHQE
                     QQQIRVQLEENELQTEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQIQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSQ
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDYAAAPQLWQHITAPTEWQHALSVILAERLHARSVPHGFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLNYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELDNLAPELSAAEAAFKQAE
                     AAVRSSETQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIVRELAQLAEE
                     QTVLQHASDGLSDDIAILQEAAAELEHQQQTAAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYQQQIAQLEQQTLDWQERQQELALAYETEFQNDEQHIKLDELT
                     EAVHTLDEEYIVVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITILEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            3577..3828
                     /locus_tag="WIE35_000498"
     CDS             3577..3828
                     /locus_tag="WIE35_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153461.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     APEPRFRQRPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            3825..4559
                     /gene="lpxH"
                     /locus_tag="WIE35_000499"
     CDS             3825..4559
                     /gene="lpxH"
                     /locus_tag="WIE35_000499"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ4153462.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLENAENAV"
     gene            4815..6401
                     /locus_tag="WIE35_000500"
     CDS             4815..6401
                     /locus_tag="WIE35_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ4153463.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6569..6772)
                     /locus_tag="WIE35_000501"
     CDS             complement(6569..6772)
                     /locus_tag="WIE35_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ4153464.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6888..7385
                     /locus_tag="WIE35_000502"
     CDS             6888..7385
                     /locus_tag="WIE35_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ4153465.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            7467..8660
                     /locus_tag="WIE35_000503"
     CDS             7467..8660
                     /locus_tag="WIE35_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ4153466.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAIRAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVALLKAKG
                     ASQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(9064..9999)
                     /gene="hemC"
                     /locus_tag="WIE35_000504"
     CDS             complement(9064..9999)
                     /gene="hemC"
                     /locus_tag="WIE35_000504"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ4153467.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10118..10633
                     /gene="ybeY"
                     /locus_tag="WIE35_000505"
     CDS             10118..10633
                     /gene="ybeY"
                     /locus_tag="WIE35_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ4153468.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAANLPSEHDFYRWAWSAL
                     KSGYRRADISLILLDEEAARAYNRDYRGKDYATNVLSFALNEGETLPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMRAA
                     GYPNPYREDGH"
     gene            10635..11459
                     /locus_tag="WIE35_000506"
     CDS             10635..11459
                     /locus_tag="WIE35_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEZ4153469.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11588..12967)
                     /gene="nhaC"
                     /locus_tag="WIE35_000507"
     CDS             complement(11588..12967)
                     /gene="nhaC"
                     /locus_tag="WIE35_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ4153470.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHCYSLIPFALLVVLALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13258..14481)
                     /locus_tag="WIE35_000508"
     CDS             complement(13258..14481)
                     /locus_tag="WIE35_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ4153471.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3531 a         4206 c         3828 g         3340 t
ORIGIN      
        1 cgatttaaat ttaattcact ataccgtctg aaaacctgta tcgcaacacg tcattaaaaa
       61 cggcaagatt cagccgttct gcaacccgtt caaacggctt ccgccatttc caacgcctgc
      121 ttgatgtcca cggcgacgac gcgcgatacg cctttttcct gcatggttac gccgaccagc
      181 tgctctgcca tttccatggt caggcggttg tgggagatgt agaggaactg ggtttgcgcc
      241 gacatttctt tgaccaggtt gcagaaacgc gaggtgttgg cgtcgtccag cggggcatcg
      301 acctcgtcca aaaggcagaa cggagcgggg ttgaggctga acagagcgaa cacgaggctc
      361 atggcggtga gggctttttc gccgccggag aggaggtgga tggtgctgtt tttcttgccg
      421 ggcggacgcg ccataatgga cacaccggcg gtcaaaaggt cgtcgcctat cattttgaga
      481 gtggcttcgc cgccgccgaa cagggtcggg aagaaggttt ggactttgcc gttgacggcg
      541 tcgaaggttt ctttgaaacg cgctttggtt ttgtcgtcga tttgggcgat ggcttcttcc
      601 aaaatggtga tggcggcctg cacgtcttcg ctttggctgc ggtagtagcc gtcgcgttcg
      661 cgcgcttctt cgagttcttg cagggcggcg aggttgaccg cgccgagggc ttcgatttgt
      721 tgcgagaggc tgccgatgct gctgttcaat actttcggcg attctttcgc caacgcttcg
      781 agcgcgtcca aatcggcggc gcgttcggtc aggttttgat ggtagcgttt ggcgttaatc
      841 agggcttcct gctgctgcaa cagggcggtt tgggtggcag cctgaagctg cggcagcttg
      901 gtttgcaagg tttgtacgcg ggcgtattgt tccctgccct gctcctgaat ctgcgcgagt
      961 ttttcctgca caacaatata ttcttcgtcc agcgtgtgta cggcttcggt cagctcgtcg
     1021 agcttgatat gctgctcgtc gttttggaac tcggtttcat aggcgagggc aagctcttgc
     1081 tggcgttcct gccagtctag ggtttgctgt tcgagttggg caatttgctg ttggtagttt
     1141 tgtttttgct ggttgagctt gtggacggcg acttcggcaa gcccgtattg gcggttggct
     1201 tccaacaagg caagctgcgc ctgtttcaga cggccttgct gctcttggcg gctgtgtgcg
     1261 gcggtttgct gctggtgttc gagttcggcg gcggcttcct gcaaaatggc gatgtcgtct
     1321 gaaagcccgt cggacgcgtg ttgcaacacg gtctgttctt ccgccaactg cgccagttcg
     1381 cggacgatgt gttcgcggcg gatttgccct tggttggtgc gcgccagaag ttcggcggcg
     1441 cgttgctgcg cttggctgta ttggcgcgtg tgttgttgtt gctgctgcat caggttttta
     1501 tgctgcgttt cagacgagcg cacggcggct tccgcctgtt taaacgcggc ttcggcggcg
     1561 gagagttcgg gggcaaggtt gtccagttcg gacgcgatgc cgtcgagacg cgctttttgg
     1621 gcaatcaggc tttcctgcgc gggtttggca tagagcagga cgctgacttt gtcgacctga
     1681 tggccttcgg gcgtgagcca gatttggtgt gcgcccaaat cgctttgatg cgcgagggcg
     1741 taattcaaat cgggcgcgca caatacgccg tcgagccagt ggtgcaatgc cgtctgaaac
     1801 ggcggctgcg cttggatttg gttcagcaat gcctgtacgg gcagggattt tttgatgccg
     1861 ccggagaggc cgtctgaaag ccacgccgcc tgcccctgcg gcaaaggcgc aggaggaacg
     1921 aagccgtgcg gcacgctgcg ggcgtgcagg cgttcggcaa gaatgacgga caaggcgtgc
     1981 tgccactcgg tgggcgcggt gatgtgttgc cacaattgcg gcgcggcggc gtaatcggtt
     2041 gcctgccaga agtcggcggc ttcctgctgt tgcgacagga tttgcgacaa cgcctgctgc
     2101 tgcgcctgca aggtaatgtg ttgctgcttc aggctttgga agcggtttga ggccgtctga
     2161 aacgcctcgc gggcggcgtg taaggcttct tcggcggcga tgatttgttc ttcgtaatgc
     2221 tcttgctggc tttgcaacaa ggcagcggcc tcctgcgcgg cggcggtttc cgcttcgtcg
     2281 ggcaggttca gggcttggtt ctcctgtttc agacggcctt tgcgctcttc gtgcttggca
     2341 acggtttgtt cggcatgggc aagctgctgc tgcttcaacg ccagttcgcg gcggatgcgg
     2401 tttgcctcat cctgctggat ttggaaggcg gcgttgagcg tggcttgggc ttcttccaat
     2461 tcgggcagac gctcttcgtg ttcggcaacc tgcatcgccc attccgccaa ttcggtttgc
     2521 ttttcttcgg tctgcaactc gttttcttca agctgaacgc ggatttgctg ctgttcctga
     2581 tggatgcgct gcatctgcgc ctgcgccgcc tgcttgtcgc gttcgatgcg ttggtgcagg
     2641 ttctgctgat ggcggatttg ttcttccaaa cgggcaatct gctcgcgcaa cacgccgcgt
     2701 ttgttgctca attcgtgtac cgcctgctgc tgcgactgtt cggcggtctg caaggcgcgt
     2761 acttcgtcgt ttaacgcctg aacctgcgcg gcggtttcgt cctgctgcgc ctgcaaagac
     2821 tgatgctgcg cggtcgcctt gtcggcggcg gcaagcgatt gctgccattg ggcgtaatcg
     2881 agcaaatcct gctgttggtt caactgcgcg gtcagggatt tgtagcgttc ggcggtttcc
     2941 gcctgtttct ccagcttttc cacctgacgc gccaattcgt tctgcaaatc gcccaaacgc
     3001 tgcaaatgct cgcgcgtgtc tttcagacgg ccttccgtct ccttgcggcg ttccttatat
     3061 ttggacacac ccgccgcctc ctcgatataa gcgcgcaact cctccggccg cgcttcgatg
     3121 atgcgcgaaa tcatcccctg ctcgataacg gcataaccgc gcgcgcccac gcccgtaccc
     3181 agaaacaaat cagtaatatc gcggcggcgc acggtctgat tgttgatgaa ataagtcgat
     3241 tcgccctgcc gcgtcagctg ccgcttgatg ctcacctcgg catactgccc ccacgccccc
     3301 tgcaaactat ggtcgctgtt gtcaaacacc agctccaccg aagccctcgg cgcaggacgg
     3361 cgcgtcgccg caccgttaaa gatcacgtcc tgcatactct cgccgcgaag ctgcttcgcc
     3421 gaagcctcgc ccaacaccca gcgcaccgcg tcaatcacat tcgacttgcc gcagccgttc
     3481 ggcccgataa ccgcaacaag ctgccccggc acatgaatcg tggtcgggtc ggtaaaagat
     3541 ttgaagccgg agagtttgat atgggtcagg cgcataatgg tcggaaaaaa taaaaaagaa
     3601 gcgtatttta acggaaatcc cgccgcacca ccccatatct tgccggcaaa accttaccgc
     3661 atcccgcacc ctcgatgccg tctgaagcct tcagacggca tttttaacgc gcccgaaccc
     3721 cgattccgcc aacgcccgat gcttggcgcg gcatttgccg tattgccccg ccgccgggcg
     3781 cgttataatc cgccacgaca tccgcccgat ttggaaacct gcctatgaaa cccgcctatt
     3841 tcatctccga cctgcatttg agcgaaaagc accccgaact gaccgcgctg ttgctgcgtt
     3901 ttttacgttc ttccgccgcc gggcaggcgc gggcggttta cattttgggc gatttgtttg
     3961 atttttgggt gggcgatgac gaagtttccg agttgaatac ttcggttgcg cgtgaaatca
     4021 ggaaattgtc cgacaaaggc gttgccgtgt tcttcgtcag gggcaaccgc gacttcctga
     4081 ttggtcagga tttttgccgg caggcaggca tgacgctgct gccggattac tcggttttgg
     4141 acttgttcgg ctgcaaaacc ctcatctgcc acggcgacac tctgtgtacg gatgacaggg
     4201 cataccaacg tttccgcaaa atcgtgcatc ggaagcggct gcaaaaactg ttcctgatgc
     4261 tgcccctgaa gtggcgcacg cgccttgccg ccaaaatcag gcgcgtcagc aaaatggaaa
     4321 aacaggtcaa gcccgccgat attatggatg tcaatgccgc ctttaccgcg cggcaggttc
     4381 gcgcctttaa cgcggaaagg ctgatacacg gacacaccca ccgcgagcat atccatcacg
     4441 aaaacggctt tacccgcatc gttttgggcg actggcataa cgactatgct tcaatcctcc
     4501 gcgtggacgg ggacggcgcg gtattcgtgc cgctggaaaa tgctgaaaat gccgtctgaa
     4561 gcctttcggg cggctttttt tacacgcccc tttcccacac tccctccgcc gcatcatcac
     4621 catccttaac ccgcattaat agccattatc gataaaatgc ttgaccgatt acataagatt
     4681 acgtaaagtg tgcaaacgca taacaattgg tcttaccaat ttatctttta accaaaatat
     4741 cgggtgtacg ggatttgaac gtctttccaa acctcgacgc gccttatgat gcaaatgcct
     4801 aaaaaggatt cgttatggca cttttcctca gcatattccc catcgtcctg ctgatttggc
     4861 tgatggtgaa aaaaaacagt atgccctcct acgtcgcgct gccgattacc gcagtgctga
     4921 tttacgccat caaacttttc tacttcgacg atgcgggaat gctgctcaac gccaccgccg
     4981 cttccggcct cgtcaaaacg ctcacgccga ttaccgtgat tttcggcgcg attatgttca
     5041 accgtatgat ggaaaccacg ggctgcatcg atgtcatccg caaatggctg gcgaccatca
     5101 gccccaaccc cgtagcgcaa ctgatgatta tcggctgggc ttttgccttt atgattgaag
     5161 gcgcatccgg cttcggcacg cctgccgcga ttgccgcgcc gattctgatg agcttgggct
     5221 tcaacccgtt gaaagtggcg attttcactt tggtgatgaa ctccgtcccc gtctctttcg
     5281 gtgcggtcgg tacgccgacc tggttcggtt tcgcaccgct gaacctgagt gccgaagaca
     5341 tcctcgccat cggcaggcag accggcgtaa tgcacttctt cgcaggtttc gtcatccccg
     5401 tcatcggctt gggcttcatc gtaccttggt ctgaaatccg caaaaacttg ggcttcgtcg
     5461 ccattgccgt cttctcctgc accattcctt atgtcgcatt ggcgatggtc aacgaagaat
     5521 tcccgtcgct cgtcgccggc gcgatcggcc tgatggtgtc cgtattcgcc gccaaccaag
     5581 gctggggctt gagcaaagac cacgccaaag acccgaatgc cgaaaaagtg ccgttcgccc
     5641 aagtcgccaa agcactcgcc cctttgggta tgctgatcgg catgctggtg gttacgcgca
     5701 tcaaacagct cggcatcaaa ggcattttga ccagcaaaga agaatggttc agcttccaac
     5761 tgccgtttga tttgtccaaa atcaccgtca gcgactccct gacgattacc ttcggcaata
     5821 ttttcggaca agatgtcagc gcgtcttacc aaacgctgta cgtcccggct tggattccgt
     5881 ttgtgctgac cgtttggatt tgcatcctgc tgtataaaac caaattcaaa gatgcctgga
     5941 cgatttattc cgtaactttc aatcaaacca aaaaaccgct gcttgccctg atgggcgcgc
     6001 tgattatggt tcagctgatg ctggtcggcg gcgacaattc gatggtgaaa atcatcggta
     6061 aggaatttgc cgcaatggcg ggcgaacact gggtttattt ctcgccgtat ctgggcgcga
     6121 tcggtgcgtt cttctccggc tccaacaccg tgtccaacct gaccttcgga ccgattcagc
     6181 agcaaatcgc cttggatacc ggcctgtccg ttaccctgat tctggcgttg cagtccgtcg
     6241 gcggcgcgat gggcaatatg gtgtgcctca acaacatcat cgccgtatgt accgtattgg
     6301 atgtgaaaaa ttccgaaggt gcgattatca agaaaaccgt tatcccgatg gcgatttacg
     6361 gcgtgattgc cgtcgtcgcg gcaatgattt tcttcctcta ggcgaaacgc caccatccat
     6421 agcggcagca cgcggggaac ggaaaccgcc gttccccgtc cgggcggcgc atcgggcaga
     6481 atctcggtgc cggaacagcc cgccccgcca aacaaatgcc gtctgaaacc ggaaaaggct
     6541 tcagacggca tttttccgcc gcttgcgttc aggcgtaatg atgatgcggc atcttgccgt
     6601 caaggcggta gcgcgtgccg caatacgggc aggcaacgct gcccgattct ccttcgcaca
     6661 aaggcaaaaa cactctcgga tgcccgttcc actgctcgtt gtcgggtccc gagcaataca
     6721 gcggcagatt ttccggcaac acggaaattt cctgcagatt cagattgtcc atttgatttt
     6781 cctttgcgtg gttggcgtgc gcccatttta cgccatcggc aggctaaagg atattttcgg
     6841 cgcaaagccg caatccgcta taatccccac ttttcagacg gcataccatg actgcgctta
     6901 cccttccgga agacatccgc caacaagagc catccgccct gctctatacc ctcgtttccg
     6961 cctacctcga acacaccgcc caaaccggcg acgaatccct ctcctgcctg tccgacgacc
     7021 agcacacgct gaccgcattc tgctacctcg acagccaagt cgaagaaggc ggcttcgtgc
     7081 aactcatcgc ctccggttac ggcgaatata ttttccgcaa cccgcttgcc gacagcctgc
     7141 gccgctggaa aatcaaagcc gtgccgaaag tcttggacaa agccaaagcc ctctacgaac
     7201 aacacggcaa aaccatcgaa acgctcgccg acggaggcgc agacatcgaa tccctgcgca
     7261 aacagttccc cgactttgaa gaatgggacg gcgcatacta cgaagccgcc gaacaagacc
     7321 tgcccctgct tgcagaacac atacagtcaa actgggaaac cttcgcccat atcgggcagg
     7381 cgtgattgcg tctgtttcca gcccgtgtaa aacagcgtaa aatcggcaaa cccgaatgac
     7441 cttccgtccc catcaaggag caagctatgt tcttcaagca catcgaagcc gcccccgccg
     7501 atccgattct cggtttgggc gaagcgttca aagccgaaac ccgccccgaa aaagtcaacc
     7561 tcggcatcgg cgtttataaa gacgcatccg gcgcgacacc cctcgtcaaa gccgtcaaag
     7621 aagccgaaaa acgcctgttg gaaagcgaaa ccaccaaaaa ctacctcacc atcgacggcg
     7681 ttgccgacta caacgcgcaa acccaaatcc tgctgttcgg caaagaccac gaaatcatcg
     7741 ccagccgtcg cgccaaaaca gcgcaaagcc tcggcggtac gggcgcattg cgtattgcgg
     7801 ccgagtttgc caaacgccag ttgaacgcgc aaaccatctg gatttccaat ccgacttggc
     7861 ccaaccacaa cgccatcgcc aaagcggtcg gtatccaaga caaaccttat cgttactatg
     7921 atgccgccaa acacggtttg gattgggacg gcatgattga ggacttgagc caagcgcaaa
     7981 aaggcgacat cgtcctgctg cacggctgct gccacaatcc taccggcatc gaccctacgc
     8041 ccgaacaatg ggaaactttg gcaaaacttt ctgccgaaaa aggctggctg ccgctgtttg
     8101 actttgccta ccaaggcttc ggcaacggtt tggaagaaga tgcctacggc ttgcgcgtgt
     8161 tcttgaaaca caatacagaa ttgctgattg ccagctctta ttccaaaaac ttcggtatgt
     8221 acaacgagcg cgtcggcgcg ttcactttgg tggccgaaga cgaagaaaca gcaatccgcg
     8281 cacacagcca agtcaaaacc atcatccgta ccttgtattc caacccggct tcacacggtg
     8341 cgaacaccat tgcgctggtg ttgaaaaatg atgatttgaa agcccaatgg attgccgaac
     8401 tcgatgaaat gcgcggccgc atcaaagcca tgcgccaaaa atttgttgcg ttgctcaaag
     8461 ccaaaggtgc aagccaagac tttgatttca ttatcgaaca aaacggtatg ttctctttca
     8521 gcggtctgac tcccgaacaa gttgaccgtt tgaaaaacga gtttgccatt tatgccgtcc
     8581 gctccggccg catcaacgtt gccggcatta ccgacgacaa catcgattat ctgtgcgaaa
     8641 gcatcgtgaa agtactgtaa aaagcacagc catatgaaaa tgccgtctga accttaacca
     8701 gttcagacgg catctgtttt ttgttaaaaa acaataaacc ataacaattt caaacaagct
     8761 aaaaacatct ataaaatcaa cccaatatga tgaactcaaa taaaaaatac gaatatcatc
     8821 attctcaaat tcaaaatttg aacgccacag tattatccga agccacaaaa acctttccgc
     8881 cgtcattccc gcgcaggcgg gaatccggga acccaatgct gcaagaattg attagaaaca
     8941 actgaaaccg agcaaactgg attcccgcct gtgcgggaat gacgaaataa ggcgagtaaa
     9001 actgatgaaa caaattgatt aaaaacaata acttatatta ttttatttaa attttcgata
     9061 aaatcaattt tccgtattca atactgctcc aatcaattcc cgcgcaccgt cgtccgccaa
     9121 tttcttagcg acagcgcgtc cgagcgcgtc ggcatattcg gcaggggctt gcgcgtccgc
     9181 ccgcaacaca accgaaccgt cggggtgtcc gaccaagccg cgcaaggtca gcaagccgtt
     9241 ttcttccgtg caatatgcgg ccaaaggcac ttggcagctt ccgcccaaag cgcgtgcgag
     9301 ggcgcgttcg gcggtaacac aggcattggt aacgccgtgg ttcaagggtt tcaaaacttc
     9361 atacaaatct tcgcggtgcg cggcaatttc gatacccaat gcgccttgtc cggcggcagg
     9421 caggctgtcg gattccgaca aaatcatgcg gatgcgtccg tccaatttca gacgctgcaa
     9481 accggcggca gccaagataa ttgcgtcgta ttcgccgtta tcgagtttgg acaaacgggt
     9541 ttgcacattg ccgcgcaaag gtttaataac caaatgcgga tagcgcgcac gcaactgggc
     9601 ttcgcggcgc aggctggatg tgccgacaac cgcgccttcg ggcatttctt ccaaacgcgt
     9661 gtattggttg gacacaaatg cgtcaaacgg attggcgcgt tcgccgatgg ccgcaagcgc
     9721 gaaaccttca ggcaaatcca tcggtacgtc tttaatcgaa tgcaccgcca aatcggcgcg
     9781 cccgtcatat aaagcctgtt ccaactcttt gacaaacaag cctttaccgc cgacttttga
     9841 caaagttttg tccaaaatct gatcgccgcg cgtggtcatg cccaaaatct cgacttcgca
     9901 atcgggatac agagccttca gacggccttg gatatgcttt gcctgccaca tagcaagcag
     9961 gctttcgcgg ctggcgatga cgagtttttt cgggttcatg ttcaatgcgt tcaaagaatg
    10021 gaaacggcgt aaagtctatc atattttgaa cgtttcttca gacggtatgc cgtggtctat
    10081 gttatattta cacccacaaa acacgaaacc cgccacaatg aaacgcacca aaaaataccc
    10141 tttcttaacg ttgcagcggc aacgtttcca tttgaacttt gaaaacgcct cttccgccgc
    10201 caaccttcca agcgaacacg atttttaccg ctgggcgtgg tctgccctga aaagcggata
    10261 ccgccgcgca gacatcagcc tgattcttct ggacgaagaa gcagcccgcg cctacaaccg
    10321 cgactaccgg ggcaaagact acgccaccaa tgtcttgagt ttcgcgctca acgaaggcga
    10381 aaccctgccc tgccaagttt cggaaaaact gtacggcgat ttgattatct gcccgcaagt
    10441 cgttttgaaa gaagccgccg aacaaggcaa aacgcccgaa cggcattttg cccacctgac
    10501 catacacggt gttttgcacc tgatgggcta cgaccacatc gaagacgatg aagccgaaat
    10561 aatggaagcc gaagaaatcc gcctgatgcg ggcggcaggc tatcccaacc cctaccgaga
    10621 ggacggacat taaaatggac ggcgcacaac cgaaaacaaa tttttttgaa cgcctgattg
    10681 cccgactcgc ccgcgaaccc gattccgccg aagacgtatt aaacctgctt cggcaggcgc
    10741 acgagcagga agtatttgat gcggatacgc ttttaaggtt ggaaaaagtc ctcgattttt
    10801 ccgatttgga agtgcgcgac gcgatgatta cgcgcagccg tatgaacgtt ttaaaagaaa
    10861 acgacagcat cgagcgcatc accgcctacg ttatcgatac cgcccattcg cgcttccccg
    10921 tcatcggcga agacaaagac gaagttttgg gcattttgca cgccaaagac ctgctcaaat
    10981 atatgtttaa ccccgagcag ttccacctca aatccattct ccgccccgcc gtcttcgtcc
    11041 ccgaaggcaa atcgctgacc gcccttttaa aagagttccg cgaacagcgc aaccatatgg
    11101 cgattgtcat cgacgaatac ggcggcacat ccggcttggt cacctttgaa gacatcatcg
    11161 agcaaatcgt cggcgaaatc gaagacgagt ttgacgaaga cgatagcgcc gacaatatcc
    11221 atgccgtttc ttccgaacgc tggcgcatcc atgcagctac cgaaatcgaa gacatcaaca
    11281 ccttcttcgg cacggaatac agcagcgaag aagccgatac catcggcggc ttggtcattc
    11341 aagagttggg acacctgccc gtacgcggcg aaaaagtcct tatcggcggt ttgcagttca
    11401 ccgtcgcccg cgccgacaac cgccgcctgc acacgctgat ggcgacccgc gtgaagtaag
    11461 cactgccgtt tctgcacagt ttaggatgac ggtacgggcg ttttctgttt caatccgccc
    11521 catccgccaa acataaaaat gccgtctgaa accgttgcaa gtttcagacg gcatttcgct
    11581 tatccgctta ttttttgctc aaagtcagcc ccgtccaacc gaataacagg gtcaaagcca
    11641 aactcaaata gcagaaaaag gcatacggca gatattccca aaccggcacg cccagcgcgt
    11701 ggctgatgaa cacgccgcat acgctccacg gtacgagcgg gttaatcacc gtccccgcat
    11761 cttccagcgt ccgcgacaga ttgcgggaat gtagtccgag cctgtcgtaa acgggtttaa
    11821 acgtttctcc agaaagcagg atgctcaaat attgttcgcc aatcaggaaa ttaaccccga
    11881 ccgaagtcat ggcaacgctg aacgtcgcgc gtccggcatt cgtcaagaag gtacggatgg
    11941 cctccaacag ggaaggaatc acaccgagcg caaacaacag tccgcccaaa ctcatcccga
    12001 gaatcacgat ggtttgcgtg aaaaacatac tttccaaacc gccgcgcgaa atcagtttga
    12061 caacatcttt aaacgcttcg ccttcgagtt tgtagccgcc gtaaaaccac gcaccgagct
    12121 gacgcagatc gggcgtgctg tgcagatacg ttacagcaac ggcaaccatg acggtaaaga
    12181 gcatggcgac gacggcgttg atgcgcatca atgccaaaac gaccaacagc gcaaacggaa
    12241 tcagcgaata gcagtgcacc aatcccgtgg cttcaagctg gctgcggaag gattcgacgc
    12301 tgttcaaatc ctgcgcggcg acgctgggaa gaagccacag catcagtgcc gcactgatga
    12361 gccacgcggg aatggtggtg tacatcatat ttttgatgtg ttcaaacagg tcgataccga
    12421 caatggacgc ggaaatgccc gtcgtatcgg aaagcgggga cattttgtcg ccaaaaaatg
    12481 cgcccgaaac aatcgcgccc gccgtcatcg ccatatcggc ctgaaacgcc gccgccatac
    12541 ccataaaggc aacgccgaca gtggcgcagg tggtcaggct gctgccgatg gacacgccga
    12601 tgacggaaca cagcgcgaag gcggaaaaat aaaaataagt cggggaaatc agtccaaaac
    12661 cgtaatacat cagcgtcgga atcgcgccgc tcatcatcag cgcgctgacc atcagcccga
    12721 tgaagaaaaa caggtaaatc gcgcccatac cctgattcaa cgcgcctatc atgccctgct
    12781 gcatatcgtt gtatttcaaa ccgcgcgcca agccgtacaa aatcagcacg acgatggcgg
    12841 caataatgga catatgcggc agccactcca atgaaatgat ggtatagccc atcgcggcaa
    12901 tcagagcgac gacgacggca agtgcctcac cgcgcggcat atcgagtaag gatttgaaag
    12961 cgaacatgat tctttgcccg atttgatata aagttaacgg attctaacac accataccga
    13021 atccgcccga cgttttcaga cggcatttta gcgttttaca aaagccggac agaaacaaaa
    13081 cggcaatttt ctcaatttaa attaattttt ttgaaatata attttttatt tttttgtttg
    13141 ttgacatata agacttttat ggattgaagt attaaaacct cggcacacac gccgcaaaca
    13201 ccggtacgca aaaatgccgt ctgaaagatg ttcagacgac atttctcaag aaaaagatta
    13261 tttgtccgcc ctatatccgt acaccgcaaa aaacacgata tacagataac agatgacgga
    13321 aacgacaaac gaagacatca gggtgtaagt atctgccacc cagccctgca cgacaggaac
    13381 gaccgcaccg ccgacaatcg cggtacacag tacaccggaa gccgcgttgg taaattttcc
    13441 caatcctttg gttgccaaag agaaaatcgt cgggaacata atcgagttga aaaaaccgat
    13501 ggcaagcagc gaccacatcg ccacatccgc attgccgcta cccgtcgcca tcgcgacggc
    13561 aagcagtacg accgcagccg atgcgttaaa cgccaaataa cggttgggcg cgaatttcgc
    13621 catcaccgcc gaaccgagga aacgtccgac catcgcgccg ccccaataga acgacaggta
    13681 atgcgcggca gaagcatgat ccagcccttt cagataaccc aatacgttga ccatcaacga
    13741 accgatagac acctccgcgc cgacatagca gaaaatgcct gccgtaccga acacgagatg
    13801 tttgtattgc catacgctgg ttttgccgtc gtggttgtgc gcgctttcct cggcggcaat
    13861 tttgcgcgcg tcgggcagcc ggatcatttt cacgaaaacg gcaaggataa tcagcagccc
    13921 cgccagtccc aaatagggaa tctgtacgga agaaatctgt tccgccttgc tgacggtttg
    13981 ggttgcgtcc gccagaatca ggaacgcgcc gatttgcggc gcaatggtcg tacccaaagc
    14041 gttaaacgcc tgaaccagcg tcagtgttgc cgattccttg ccgggtttcg ccagcagggt
    14101 aacataagga ttaccggcga cctgaagcag cgttacgccg gaagccaaaa taaacaacgc
    14161 gcccaaaaat accgcgtaag aatggctacc cgcagcagga taaaacagca ggcatccgac
    14221 cgccgtcagc agaaagccgc cgataacgcc gtttttgtag ccgactttgc cgacaaaagc
    14281 ccccatcggg atggacatca ccgcataggc ggtaaagaaa cagaattgga tcagcatcgc
    14341 ctgaacgtaa gacaggtcga aaatttcttt caaatgaggg ataaggatgt cgttcatgca
    14401 ggtaataaaa cccatcatga agaacagcgt ggtcaagacg accaatgggg acgtatgatt
    14461 gttttgtgat tgcgcagaca tgatttgcct ttcgtggtgt cggaatatta cccgtttcaa
    14521 gaaaatagaa attgacattt cttaacggca atgcaaatca tactgatatt tgaacatttt
    14581 gcaaacttta tcggatgcag gcaatgcgcc gatctgaaat tcaagcagca gaaattaata
    14641 ttttacagtt gcttgcatag attttaatag attttaaaaa attccggcgg acttgcaaaa
    14701 accgttccca ataaaatgaa aatccgccgc cgtatccgga aaattcggaa atgccgcatc
    14761 gtggcatcaa aaccggagcg gaaagaatgt cgtatgcccg acaaaaaagg ggcatacggc
    14821 tgtgccgtat tttttgcacc gtcctccgcc cttcggcggg ggcgtgaaaa tatagtggat
    14881 taacaaaaat caggacaagg cgacg
//
LOCUS       DAWHXZ010000025        14686 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000025 DAWHXZ010000000
VERSION     DAWHXZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14686
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.025"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1485
                     /gene="ppx"
                     /locus_tag="WIE35_000509"
     CDS             <1..1485
                     /gene="ppx"
                     /locus_tag="WIE35_000509"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ4153472.1"
                     /translation="LASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFAAGLDEQKN
                     LSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAEAALGFPIE
                     IIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITESLPLGCVT
                     YSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKSIRDVLAAE
                     MPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMAAFEEMKLD
                     RMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAETAQTFMDS
                     LCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADMPGFSRKEQ
                     TILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFPKNMQLRTD
                     TESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEVV"
     gene            complement(1694..2059)
                     /locus_tag="WIE35_000510"
     CDS             complement(1694..2059)
                     /locus_tag="WIE35_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153473.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAKEAVTEAKEAVTEAAKDTL
                     NKAADATQEAADKMKDAAK"
     gene            complement(2379..2720)
                     /locus_tag="WIE35_000511"
     CDS             complement(2379..2720)
                     /locus_tag="WIE35_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153474.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNDLEAVSGKCMETDDKGGPVGW
                     AENGVCHTLFAKLVGNIAEEGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            complement(2726..3736)
                     /gene="trpS"
                     /locus_tag="WIE35_000512"
     CDS             complement(2726..3736)
                     /gene="trpS"
                     /locus_tag="WIE35_000512"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ4153475.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFKEIFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAAFTQMLAEGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            3997..6576
                     /gene="clpB"
                     /locus_tag="WIE35_000513"
     CDS             3997..6576
                     /gene="clpB"
                     /locus_tag="WIE35_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ4153476.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LEKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            complement(6663..7877)
                     /locus_tag="WIE35_000514"
     CDS             complement(6663..7877)
                     /locus_tag="WIE35_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ4153477.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIHDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(7966..8175)
                     /locus_tag="WIE35_000515"
     CDS             complement(7966..8175)
                     /locus_tag="WIE35_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ4153478.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLAHVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            8520..9326
                     /locus_tag="WIE35_000516"
     CDS             8520..9326
                     /locus_tag="WIE35_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ4153479.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            complement(9384..10649)
                     /locus_tag="WIE35_000517"
     CDS             complement(9384..10649)
                     /locus_tag="WIE35_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ4153480.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKIVAVSTVDVAIYNENGLDMEAIIKEFQTKGFITNE
                     AGYGKEISNAELLALEVDVLAPCALENQLTSENAGKVRATIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            11056..11763
                     /locus_tag="WIE35_000518"
     CDS             11056..11763
                     /locus_tag="WIE35_000518"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ4153481.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLSNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAAELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            11820..12281
                     /gene="recX"
                     /locus_tag="WIE35_000519"
     CDS             11820..12281
                     /gene="recX"
                     /locus_tag="WIE35_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ4153482.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IDVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            12355..12516
                     /locus_tag="WIE35_000520"
     CDS             12355..12516
                     /locus_tag="WIE35_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153483.1"
                     /translation="MSEQPEKHHTSPIEDERKNPVYRMGRAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            12669..13151
                     /locus_tag="WIE35_000521"
     CDS             12669..13151
                     /locus_tag="WIE35_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ4153484.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(13358..14605)
                     /locus_tag="WIE35_000522"
     CDS             complement(13358..14605)
                     /locus_tag="WIE35_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ4153485.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKTAAVK
                     SRPAVPAAAQPPVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNML
                     SIGQIVKVKPAGYAAPKTAAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKA
                     VPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAAD
                     GKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDAS
                     RTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT         3677 a         3997 c         3674 g         3338 t
ORIGIN      
        1 ctcgcctccg tcgatttggg ttccaacagt tttcgcctcc agatttgcga aaacaacaac
       61 ggacaattaa aagtcatcga ttcgttcaaa cagatggtgc gcttcgccgc cggactggac
      121 gaacagaaaa acctgagtgc cgcttcccaa gaacaggctt tggactgtct ggcaaaattc
      181 ggcgaacgcc tgcgcggctt ccgccctgaa caggtacgcg ccgtggcaac caatacattc
      241 cgcgtcgcca aaaacatcgc agatttcctt cccaaagccg aagcggcact gggtttcccc
      301 atcgaaatca tcgccgggcg cgaagaggcg cggctgattt ataccggcgt gatccacacc
      361 ctccccccgg gcggcggcaa aatgctggtt atcgacatcg gcggcggttc gacagaattt
      421 gtcatcggct cgacgctgaa tcccgacatt actgaaagcc tgcccttggg ctgcgtaacc
      481 tacagcctgc gcttcttcca aaacaaaatc accgccaaag acttccaatc tgccatttcc
      541 gccgcccgca acgaaatcca gcgcatcagc aaaaatatga ggcgcgaagg ttgggatttc
      601 gccgtcggca catcgggttc ggcaaaatcc atccgcgacg tgcttgccgc cgaaatgccc
      661 caagaggcgg acattaccta caaaggaatg cgcgccctcg ccgaacgcat catcgaagcc
      721 ggttcggtca aaaaagccaa atttgaaaac ctgaaaccgg aacgcatcga agtttttgcc
      781 ggcggacttg ccgtgatgat ggcggcgttt gaggaaatga aactcgacag gatgaccgta
      841 accgaagccg ccctgcgcga cggcgtgttt tacgatttga tcgggcgcgg tttaaacgaa
      901 gatatgcgcg gacaaacggt tgccgagttc caacaccgct accacgtcag cctcaatcag
      961 gcgaaacgca ccgccgagac cgcgcaaacc tttatggaca gcctctgcca cgctaaaaac
     1021 gttacagttc aagagcttgc cttgtggcaa cagtatctcg gacgcgccgc cgcgctgcac
     1081 gaaatcggtt tggacatcgc ccacaccggc tatcacaagc attccgccta catcctcgaa
     1141 aacgccgata tgccgggttt ctcacgcaaa gaacagacca tacttgccca actggtcatc
     1201 ggtcatcgcg gcgatatgaa aaaaatgagc ggcatcatcg gcaccaacga aatgttgtgg
     1261 tatgccgttt tgtccctgcg ccttgctgcc ctgttctgcc gttcgcgcca agacctgtct
     1321 ttcccgaaaa atatgcagtt gcgcacggat acggaaagct gcggcttcat cctgcgtatt
     1381 gacagggaat ggctggaacg ccatcccctg attgccgacg cattggaata tgaaagcgtc
     1441 caatggcaaa aaatcaatat gccgtttaaa gtcgaggtcg tctgaacctt acggaacaaa
     1501 tgccgtccaa accctgtcca gacggcattt gcctgtccgc aacatcccga tatgcgcggc
     1561 acatctgctc ggaacggtca tgcaggcgta aaaaacaagg ggcatacaac tcaaaaaccg
     1621 cctgaaaatc ttcaggcggt ttcgtttggg ttgccggcag gcggcatccc atcatttttg
     1681 ccaaggcaac aaattatttg gcggcatctt tcattttgtc tgccgcttcc tgagtcgcgt
     1741 cggcagcttt gttcaaagta tctttagctg cttcagttac agcttctttg gcttcagtta
     1801 cagcttcttt ggcttcagtt acagcttctt tggcttcagt tacagcttcc tcggcacttg
     1861 cctttgcatc agccgcagca tctttgactt ggtctttcgc ttcttcgacg gcagaagcgg
     1921 cagactcggc ggcagaagcc gcagtgtctt taacatcgga ctcaacggct tgaaccgctt
     1981 ccttaacctc ctgtttggct tcttgcgaac aagctgccaa ggcagccgcc atcattgcgg
     2041 caatcaataa ttttttcatg tcttatcctt cttgagttgt tgattaaggt tttgcttaaa
     2101 aatcgaaccg tgttccatca atcggctgat tttgcccatc ggccggagag aaaacggttt
     2161 cccgtttagt taaaacccat tatatttaaa tataaaggtt tttttctcga acaataaggc
     2221 ggcatcaatg ccatattgaa acacgtccga aaactatttt atgaaaacag ttcggaaaat
     2281 tgtaacacat atccccctcc ttttgagttt ccctgcggtg cggacttttt ttcgcaaggt
     2341 ttgaaaaacc caaatatatt ccgggatgtc cgaatacctc aataatggcg gcggcggaaa
     2401 taaaacgccc cctcgctgtc gatttccagc acatagcgtc cgttctgcac ggcggcatag
     2461 ccgcttttgc ctgcctgata gggttgcagg gcggaatgcg aaatcaggta atccgtcagt
     2521 ttgccgcctt cttcggcgat attgcccacc agtttggcaa acaaggtatg gcacacgccg
     2581 ttttccgccc agcctaccgg accgccctta tcgtcggttt ccatgcattt gccgctgacg
     2641 gcttccaagt cgttgggatg cttgccggtc agccggacga cgttttgttc cggcaagcct
     2701 ttaagaggtt cggacggcat cgggtttatt tcaacgggcg gatgccgacc gcatcgcgta
     2761 ctttgtccaa caattcgcgt gcttctttac gcgctttttg cgcgcctgcc tgcaaaatct
     2821 cttcgatttg cgaagggtcg gcggtcagct cgttgtagcg ttcgcgcggt tcggcgagtt
     2881 cggcgttgat tttcgccgcc aaaagttttt ttgcctcgcc ccaagccaag ccttcggcaa
     2941 gcatttgcgt gaacgctgcg gtttcagacg gcgtggagaa ggctttgtag atttcaaaca
     3001 atgggctttc gtcgggctgt ttcggctcgc ccggctcttt catattggtg atgattttgt
     3061 tgaccgattt ttgggttttt ttgtcgtttt cccaaagcgg aatggtgttg ccgtaggatt
     3121 tggacatttt gcgtccgtcc aaaccgacca agagttcgac gttttcgtcg attttcactt
     3181 cgggcagggt aaagatttct ttaaagcggt gattgaagcg gccggcgatg tcgcgcgcca
     3241 tttcgacgtg ttggatttgg tcgcgcccga cgggcacttc gttggcgttg aacataagga
     3301 tgtcggcggt catcagaatc ggatagctga acaaacccat ttccacgccg aaatcggggt
     3361 cttcctgccc gttttccgca tttgcctgca cggcggcttt gtaggcgtgg gcgcggttca
     3421 tcaaaccctt ggcggtgatg caggtcagaa tccagttcaa ttccatcact tcgggaatgt
     3481 cgctttggcg gtagaaggtg gtgcgctcgg ggtcgagtcc gcaggcaagc caagtggcgg
     3541 caacggcttg ggtggattgg tgaatcatct ccggctcgtg gcatttgatg ataccgtggt
     3601 aatcggcgag gaagaggaag gattcggtat cagggttttg cgccgcgcgg acggcggggc
     3661 ggatagcacc gacgtagttg cccagatgcg ggatgccggt ggtggttacg ccggtcagaa
     3721 ctcgtttttt gctcataaaa atgtccttgc ggcatcaatg ccgtctgaaa gggaaaaaga
     3781 tgcgccgatt atacccgatt tgccacctac atccagccga caacagactt ttccatatta
     3841 agaagatata gttatacaca ttattataca tttttatata ctttaaattc aatgatatat
     3901 cgaattaaat atagaaaaac agaaaacaga acttgagtta tccacaatta tgcacatata
     3961 ggcttcgaca gcggacattt tgaaaaggaa acaaaaatgc gatacgacaa attaaccgcc
     4021 aaattccaac aggcccttgc agaagctcag agtttggcgt tggctgcgga cagcagctat
     4081 ctggaagcgg gctttgtgct gaaagccctg cttgacgacc aaaacagcgg tgctgccgcg
     4141 ctcttggctc atgcgggcgt gaacgtgccg caggtgaaac agcgtttgca gcagcatttg
     4201 aacagcctgc cgaaagtgtc cggccagggc ggcgacattc tgcccagccg cgaattgcag
     4261 gcggtgttga acctgatgga caaagccgca accaaacgcg gcgatgccta tatcgccagt
     4321 gaacttttcc tgcttgcctt ggtgcagcag aacgatgcgg ccggcaaaat tttgaaagaa
     4381 gccggcgcga ccgaacaaaa catcaatgcc gcgattgacg cggtacgagg aggacaaaac
     4441 gtgaacgatg ccaatgccga agaccaacgc gatgctttga aaaaatatac gctcgatctg
     4501 acccaacgcg cccgagacgg taagctcgac cccgttatcg gccgtgacga cgaaatccgc
     4561 cgtgcgattc aggtattgca acgccgtacc aaaaacaacc ctgtgctgat tggcgaaccg
     4621 ggcgtgggta aaaccgccat cgtcgaaggt ttggcacaac gcatcgtcaa cggcgaagta
     4681 cccgaatccc tgcgcaacaa acgcctactg gttttggact tggcggcatt gattgccggc
     4741 gcgaaatacc gcggcgaatt tgaagaacgc ttgaaaggcg tgttgaacga cttggcgaaa
     4801 gacgacggca acactctgat tttcatcgac gaaatccata ctttggtcgg cgcaggcaaa
     4861 accgacggtg cgatggatgc gggcaatatg ctgaaaccgg ctttggcacg tggcgaattg
     4921 cactgtatcg gcgcgaccac cttggacgaa taccgccaat acatcgaaaa agacgcggca
     4981 ctcgaacgcc gcttccaaaa agtattggtt ggcgagccaa gtgtagaaga caccatcgct
     5041 atcttgcgcg gtttacagga gcgttatgaa atccaccacg gtatcgatat taccgaccct
     5101 gctatcgttg ccgcagcgga gttgagcgac cgctacatta ccgaccgctt cctgcctgat
     5161 aaagcgattg atttgattga cgaagccgcc agccgtgtca agatggaaaa agaaaccaag
     5221 ccggaagcaa tggacaaaat cgaccgccgt ctgattcagc ttcggatgga aaaggcgcac
     5281 gttgaaaaag aaaaagacga tgccagcaaa aaacgtttgg aactgataga tgaggaaatc
     5341 gacggtttgg aaaaagaata cgccgatttg gacgaaatct ggaaagccga aaaagcgatt
     5401 tcagacggtg ctgctaatat taagaaacaa attgacgaag tcaaaattaa aatcgaacag
     5461 gcaaaacggc aaggcgattt ggcactggct tcaaaattga tgtatgaaga tttggagcat
     5521 ttggaaaaac agcgtgcagc cgccgaacgg gcagatacgg acagcacaaa accggcaaac
     5581 aaactcttgc gtaataatgt cggcgcagag gaaatcgcag aagttgtttc ccgtatgacc
     5641 ggcattcctg tgtcaaaaat gatggaaggc gaacgcgaca agttgctgaa aatggaagaa
     5701 gtgttgcacc gccgcgtagt cggtcaagac gaagccgtgc gtgccgtgtc cgacgctatc
     5761 cgccgcagcc gttccggtct tgccgacccg aataaaccct acggcagctt cctgttccta
     5821 ggcccgaccg gcgtgggtaa aaccgagttg tgtaaggcat tggcaggctt cctgtttgac
     5881 agtgaagatc atctgattcg tatcgatatg tccgaatata tggaaaaaca cgccgttgcc
     5941 cgcctcatcg gcgcgcctcc gggctatgtc ggctacgaag aaggcggcta cctgaccgaa
     6001 caagtccgcc gcaaaccgta cagcgtgatt cttttggacg aagtggaaaa agcccatccc
     6061 gacgtgttca acatcctgct gcaagtattg gatgacggtc gtttgaccga cgggcaaggc
     6121 aggacggtag attttaaaaa taccgtgatt gtgatgactt ccaacatcgg cagccagcac
     6181 atccaacaaa tgggcattca ggattacgaa gcggtaaaag aagttgtgat ggaggatgtg
     6241 aaagaacatt tccgtccaga aatgatcaac cgcatcgacg aagtagtcgt gttccacgga
     6301 ctggatcagg caaacatccg cagcattgcg aaaatccagc tcaaaggttt ggaaaaacgt
     6361 ttggaaaaac aaaacctgcg cctgactgtt tccgatgccg cactggacat catcgccaaa
     6421 gccggtttcg acccgattta cggcgcacgt ccgctcaaac gcgccatcca gtcggaaatc
     6481 gaaaacccgc tggcaaaagc cctgcttgcc ggaaactatg cgcccgaaag cgaaatcagg
     6541 gtagaagccg acggcgacag actgaaattt gcctgattcg ttcctgctgt tgaaaatgcc
     6601 gtctgaaacg ggaatctccg tttcagacgg cattttttat cctcggcaga caaaccgtcc
     6661 ccttattggc ggtaggtttg caggaatctt gccagcctgc ccatcgcctc ttcaatctga
     6721 tggacgtaag gcagcgtgac aatgcggaaa tggtcgggct tgatccaatt aaaccccgtt
     6781 ccctgcacca gcaagacttt ttcgcgcacc agcaaatcgt aaacgaattt catgtcatcg
     6841 tggatacggt acatttcggt atcgattttt gggaacatat acatcgcgcc catcggtttg
     6901 acgcaggata cgccgggtat ctgattgacc aattcccacg ccctgttacg ctgttccaaa
     6961 agccgtccgc cgggcaaaat gaattcgtta atgctctgat aaccgcccaa tgccgtctga
     7021 atcgcatgct gcatcggcgt attcgcacac aagcgcatgg acgagagcat atccaaaccc
     7081 tcgatgtaac cttttgcatg atgtttcggc ccgttgagca ccatccagcc ttggcggaat
     7141 ccggctacac ggtaggcttt ggacaaaccg ttgaacgtta ccgtcaaaag gtcgggggcg
     7201 agcgcggcga tgtggtggtg aaccgcgccg tcataaagga ttttatcgta aatctcgtcg
     7261 gcgaaaataa tcaaaccgtg cttgcgcgcc agttcggcga tttccaacag gatttccctg
     7321 ctgtacaccg cgcctgtcgg attattggga ttgatgacga cgatggcttt agttttgggc
     7381 gtgattttgg cttccatatc ggcaaggttg gggaaccagc cgttttcttc gtcgcacaga
     7441 taatggcgta ccgtaccgcc cgcaagcgtt gccgccgccg tccacaaagg gtagtcgggc
     7501 gcgggaatca ggatttcgtc gccgtcgttg agcaatgcct gcatagacat cgtaatcagc
     7561 tcggacacgc cgttgccgat atagacatca tcaaccgtaa tatcgcgcaa acctttggtc
     7621 tgatagtagt gaacaatggc tttgcgggcg gaatacagcc ctttggaatc gcaatagcct
     7681 tgcgaagtcg gcaggttgcg gatgacatcg accaagattt catcaggggc ttcaaagccg
     7741 aacggcgcag ggttgccgat attgagttta aggattttat tgccctcctc ttccaactga
     7801 agggcttttt tgtgaaccgg cccgcgtatg tcgtaacaga cgtgatcgag ctttgcagac
     7861 ttgggaaatt tatccatgat gtgttccgta aattttgggc aatgggtggg aatgtactct
     7921 ttttcacgcg gaatttaaag catcaaaccg agattttcag gctttttacc tgccctcttt
     7981 gcgccgttcg ctgacgcttt tgccgcctat tccccagtta tcggtatcca cttcgtcaat
     8041 cacgacaacc gttgtttcgg gatttttacc cagcacgtgt gccagcaatt cggttacgcc
     8101 gccgatcagt tccgcttttt gcgcggcagt cggtgcttcc ttgccgccgg ttactttaat
     8161 attgacataa ggcatgatct ttctccgttt tgaaatattg ctatcttatc aaacaagttg
     8221 cctccgccca aacgtccgct tcattttctg aaaaattcaa atcgatatag cggattaaca
     8281 aaaatcagga caaggcggcg aagccgaaga cagtacaaat agcacggaac cgattcactt
     8341 ggtgcttcag caccttagag aatcgttctc tttgagctaa ggcgagccaa cgccgtactg
     8401 gtttttgtta atccactata caaaaagaca gttttcagac agcaaatccg tcttcacacg
     8461 atacctattt tgttataaca taacaaaatc tttaacccac acgagacaaa ggctgcacca
     8521 tgaagaaaac attgacactg ctcgccgttt ccgccctatt tgccacatcc gcccacgccc
     8581 accgcgtctg ggtcgaaacc gcccacacgc acggcggcga ataccttaaa gccgacttgg
     8641 gctacggcga atttcccgaa ctcgaaccca tcgccaaaga ccgcctgcac atcttcagca
     8701 aaccgatgca gctggttacc gaaaaaggca aggaaaacat gattcaacgc ggcacataca
     8761 actaccagta ccgaagcaac cgtcccgtta aggacggcag ttacctcgtc atcgccgaat
     8821 atcagcctac tttctggtca aaaaacaaag caggctggaa acaggcgggc atcaaagaaa
     8881 tgcctgacgc aagctattgc gaacaaaccc gtatgttcgg caaaaacatc gtcaacgtcg
     8941 gacacgaaag cgcggacacc gccatcatca ccaaaccggt cggacaaaac ttggaaatcg
     9001 tcccgctgga caatcccgcc aacattcacg taggcgaacg cttcaaagtc cgcgttctgt
     9061 tccgtggcga accgctgccc aatgccaccg ttaccgccac ctttgacggc ttcgacacca
     9121 gcgaccgcag caaaacgcac aaaaccgaag cacaggcttt ctccgacagc acagacgaca
     9181 aaggcgaagt ggacatcatc cccttgcgcc aaggcttctg gaaagccaat gtcgaacaca
     9241 aagccgactt ccccgatcaa agcgtgtgcc aaaaacaggc gaactactcg actttaacct
     9301 tccaaatcgg ccattcgcac cattaatccc gcccgcacaa aaatgccgtc tgaaggcttc
     9361 agacggcatt ttttgttcaa acatcaatac caaccgcgca gtttcatcgc tttttcaaca
     9421 cggcggatac tcatcatata agacgcggtt cgcaaatcga catcgtattc ttgagccaag
     9481 ttccaaatat cgcggaacgc tcggcgcagg acgacggttt ctttctcttg aacttcgtca
     9541 aactcccaat aatagccttg caggttttgc acccactcga aataggaaac gaccacgccg
     9601 ccgcagttcg ccagaatatc aggcacgacc aatacgccgt tttgacgcag gataacgtct
     9661 gcctctggag tagtcgggcc atttgcgcct tcgaccacga ttgtcgcgcg gactttaccg
     9721 gcattttcag aagtcagttg gttttccagt gcgcaagggg caagtacgtc cacttccaga
     9781 gccaaaagtt cggcattgct gatttcttta ccgtagccgg cttcattggt gatgaaacct
     9841 ttggtttgga attctttgat gattgcttcc atatccaagc cattttcatt gtagatggca
     9901 acgtcaacag tagaaacggc aacgattttc gcaccggatt gatgcgcgta ataacctgtg
     9961 tggtagccga cattaccgaa accttgaatg gcgtaagtgg cacccttcac gtctttgccc
    10021 agtttttcca aagcttggac ggcagcgaga ttcacgccgt aaccggtggc ttcggtacgc
    10081 gccaaagagc cgccgaactc aaccggtttg ccggtaaata cgcccggtgc agaatgtttc
    10141 accacgtttt cataggcatc aaccatccaa gacatgattt tgccgttggt gttcacatca
    10201 ggtgcgggaa tatcgatttt ctcgccaatc agcggagaaa tggcttcgga ataagcgcgg
    10261 gcgatgcgtt ccagttccgc ctcggaataa tcgcgcggat ccaaggtaac gccgcctttg
    10321 ccgccgccat aaggaatacc cgcaacgcag catttgatgg tcatccaaat cgacagggct
    10381 ttgacttcgt ccagattcac accaggatga aagcgcacgc cgcctttata ggggccgacg
    10441 gcgttgttgt gttgcgaacg gtagcccgtg aaggttttga ccgtgtcgtc gtcgagtttg
    10501 acggggaaat tgacttccaa cacgcgggtc ggactcttca ggatttcata aacggccgga
    10561 tcggttttca gccggtcgca ggcggttttg acctgtttgc gcgcgatttc aaacggattg
    10621 agggtttctt ttgcaagggc ttcagacatt ttgcttcctt ttcacaaaaa gattttcagg
    10681 ggcgtgcatt tcgctcatta ctataaccaa tttcccgaca aaatgtaata gcgtgtagtc
    10741 ggcataagcc cgatttgatt aatctatata tgattttatt tttcaagccg cacggaatca
    10801 gcccggactc cgcctgaaaa agcagcgcgc gcatccgaaa aataaatgtc aaattaaata
    10861 aagatatagg catcctttta ttttttaaaa atttaattgg aacatcgccg ggatttcaaa
    10921 cccttgcccg attccgcccc gaaatccgaa aacaccgcct gcaaaacctg ttgcgattgt
    10981 taacaatcta tacattagaa gccctgtgca aacgatgtta aaataaaccc tttcaacccg
    11041 acaggaaatc agattatgaa tgcagccatc gaacacgtcc aagccgtcgc cttcgatttg
    11101 gacggcacac tgtgcgattc cgtccccgac cttgccgccg ccgcagaagc gatgttggaa
    11161 caactcggta tgaaaccgct gcccgccaaa gtggtcgaaa gctatgtggg cgacggcatc
    11221 ggcaaactgg ttcaccgcgt cctctccaac gaccgcgacc gcgaagccga ttccgaactg
    11281 tgggaaaaag gtttcgtatc ctatatgaaa tactaccgcg accatttgag cgtcttcacc
    11341 cgcccctatc ccgaaaccga agccgggctg gcattgctca aatctttggg catcccgctg
    11401 gcagtcatta ccaataaaaa cgaaatcctt gccgccgaac tgttgaaaca gctcggactt
    11461 gccgactact tcagcctgat actcggcggc gacagcctgc ccgaaaaaaa acccagcccc
    11521 ctgccgctgc ggcacgccgc cgaagttttg ggtatcgatg ttgcaaacat ggttatggtc
    11581 ggcgactcgc gcaacgacat catcgccgcc aaagccgccg gctgcctgag cgtcggcgtt
    11641 accttcggtt acggcgatat gacgctgctc tcgcaagacg atgcgacccg ccccgactgg
    11701 attatcggct cgctgcccga aatttacgaa aacctgcaac cgcagaaaaa taaagaagag
    11761 taggcattcg gacggcacgc tatgccgtct gaaacctgcc gcacgccgaa actgccgcca
    11821 tgaaaccgca aaaatcccta cgcgcccgcg cgatggacat cctctcgcgc caagaagtca
    11881 gccgcatcgg tctgaaacgc aaacttgcac cgcacgccga aagcgaagaa gagttggaaa
    11941 acgtgttaaa cgaatttgcc gaacgcaact ggcagtcgga tttgcgctat gccgaagcct
    12001 atatccgcag caaaagccgc aaacacggtt cattgaggct gaaacaggct ttggcgcaac
    12061 agggcataga tgaagaaacc agccgcaacc tgcttcccga ccgctcaagc gaaaaacagg
    12121 ccgccataga cgtgttgcgt aaaaaattca aacaccccgc cgccgacctt aaagaaaaac
    12181 aaaaacaggc gcgtttcctc gcctatcgcg gattcgatgc cgataccgtt cagacggcat
    12241 tgaaacacgc ttgggacgaa aattgggaag acggctgctg accttccttc ttgaatcttt
    12301 ttgcacggca acataacctt acgcccgttt ccaacttttc cgattgagaa caaaatgtcc
    12361 gaacaacccg aaaaacacca cacctccccc atcgaagacg aacgcaaaaa cccggtttac
    12421 cgtatgggac gggctgttgc cggattcatg ctcgtcgttt gggcgggcgt attggcactc
    12481 gtgtttttcc tggtcttccg tttttggctt tcctaaacaa aatgccgtct gaaaccttca
    12541 gacggcatcg gcagcccatt tcggcaggct atcccatcat agcttttttt agcttgaatt
    12601 ccactttccc attccctaaa atttttccac acccatttca aaataccctt tcttaaaaca
    12661 ggtacactat gacacaacaa cgccaactgc cttcccacga actcattatg tccgaactga
    12721 tgatgccgga caccgccaat ttcagcggca acgtacacgg cggcgaactc ctgctcctgc
    12781 tcgaccaggt tgcctattcc tgcgccagcc gttacagcgg caattattgc gttaccctgt
    12841 cggttgacaa agtcctgttt aaagaaccca tccacatcgg cgatttggtt accttttacg
    12901 ccgctgtcaa ctacacggga cgcacctcga tggaaatcgg catccgtgtc gaagcacaaa
    12961 acatccgcac cggcgaaatc cgccacacca acagctgcta cttcacgatg gttgcggtca
    13021 aagacggcaa acccgtccct gtccctccgc tggaaatcct gaccgaccgc caacgctgcc
    13081 gctacgaaaa agccaaaaaa cgcagagaca tcagcctgca agcctctgaa gacatgtcct
    13141 gcggctgctg acggcggact atgccgtctg aaagacaggc acatcgcgcc atccgtttct
    13201 actgcaaacg gatgaaatca agcaaatacc caaacacagt caaacaaatt tatatgcccc
    13261 gtcccttccg aatgatttga aaacacagcc gccaaaaaca aaaatgccgt ctgaaaacct
    13321 ttcagacggc atttccaact tgatttcagg cagaaagtca gaacgcgata tagctgttcg
    13381 ggttaaccgg tttgccgttt tgccgcacct cgaaatgaag ctgcgttctg gaagcgtcgg
    13441 tattgcccat caaagcgacc tgctgcccgc gtttgacctg ctggccttcg ccgaccagca
    13501 atttttggtt gtgcccgtat gcggtcagga aggaagaatt atgctggatg atgaccagat
    13561 tgccgtatcc cctcaaaccg gaacctgcat aaaccacttt gccgtcagcc gccgccaaaa
    13621 cgggctgtcc cgcattaccg gcaatatcga cacccttgtt gttgccgccg aaatcggcaa
    13681 ccactttacc ttgcgtcgga cgctgccaaa caatgccgcc gaccgaacgc gttccggaag
    13741 gcgaagcggc aggagattgc ggggcgggcg cgggaaccgc tttattttcc gcagcgggcg
    13801 cggcaggttg cggcgcggac tgcacaggcg gttgcgcggc gggtttcaca ggggtttgca
    13861 cggcagccgg tacggcgggc ctgctttcta cggctgcggt tttcggtgcg gcatatcctg
    13921 ccggtttgac tttaacaatc tgaccgatgc tcaacatatt gtcggtcatg ccgttccacg
    13981 cacggaaatc gtcttgagag atatggtagc gtttggaaat gttgtacacc gtgtcgccgc
    14041 gcacaatagt atgcgtcgcc gcgttaatgt cgacgggtgc ggactgtacg ggcggttgcg
    14101 cggcagccgg tacggcgggc ctgcttttta cggctgcggt tttcggtgcg gcatatcctg
    14161 ccggtttgac tttaacaatc tgaccgatgc tcaacgtatt gtcggtcatg ccgttccacg
    14221 cacgaaaatc gtcttgagag atatggtagc gtttggaaat gttgtacacc gtgtcgccgc
    14281 gcacaatagt atgcgtcgcc gcgttgatgt cgacgggtgc gtaagaagga acgtatgtac
    14341 ccgaaacggc aggtgcagac ggcggaacat aagcaggagg cgtataaacc ggcgcgcttt
    14401 gcaccggcgg cacataaggc gcatcgccgg caggagccgg gctgtacggc gttgctccat
    14461 aggggttgtt gtaaactgcc gaagacggcg cgtcctgcat acctgaattg cctgcaatga
    14521 cgggggcagg ctgctgggta gcacaaccgc ccaacagtgc ggcaaaggag gcgcaagccg
    14581 ccaaaagtgt cgtttgtttc aacataagat aaccttcatg ttccgatata tagcctgaat
    14641 gcggtatatc ataataaaaa tgcgcgttct tctcaagcgc aaagcc
//
LOCUS       DAWHXZ010000026        14013 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000026 DAWHXZ010000000
VERSION     DAWHXZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14013
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.026"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1743
                     /gene="recD"
                     /locus_tag="WIE35_000523"
     CDS             <1..1743
                     /gene="recD"
                     /locus_tag="WIE35_000523"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ4153486.1"
                     /translation="ERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQNG
                     HSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAAD
                     TSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLAL
                     ICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLLK
                     LRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLPS
                     VGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGDN
                     SGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGNI
                     EAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYAL
                     ELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEYR
                     EVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTRQ
                     TALGSMLERVFSQE"
     gene            complement(1815..2918)
                     /locus_tag="WIE35_000524"
     CDS             complement(1815..2918)
                     /locus_tag="WIE35_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ4153487.1"
                     /translation="MSSFIFPDNGAEWRNESVQKPPKGWHYVRESGADGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRARKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPVHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            3169..5631
                     /gene="lon"
                     /locus_tag="WIE35_000525"
     CDS             3169..5631
                     /gene="lon"
                     /locus_tag="WIE35_000525"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ4153488.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAALEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAAAESASKPKPRSRATKH"
     gene            5813..6082
                     /locus_tag="WIE35_000526"
     CDS             5813..6082
                     /locus_tag="WIE35_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ4153489.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(6141..6575)
                     /locus_tag="WIE35_000527"
     CDS             complement(6141..6575)
                     /locus_tag="WIE35_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153490.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLTVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(6568..7875)
                     /locus_tag="WIE35_000528"
     CDS             complement(6568..7875)
                     /locus_tag="WIE35_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ4153491.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEEKNVMVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGHDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            8048..8443
                     /locus_tag="WIE35_000529"
     CDS             8048..8443
                     /locus_tag="WIE35_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ4153492.1"
                     /translation="MMRRRGKKMLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            8520..10442
                     /locus_tag="WIE35_000530"
     CDS             8520..10442
                     /locus_tag="WIE35_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ4153493.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKFTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIIRSEKLNIGYFAQHQLDTICADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIAVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSLLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            10908..11423
                     /locus_tag="WIE35_000531"
     CDS             10908..11423
                     /locus_tag="WIE35_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153494.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(11931..12590)
                     /gene="ung"
                     /locus_tag="WIE35_000532"
     CDS             complement(11931..12590)
                     /gene="ung"
                     /locus_tag="WIE35_000532"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ4153495.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            12671..13078
                     /locus_tag="WIE35_000533"
     CDS             12671..13078
                     /locus_tag="WIE35_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ4153496.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIAKP"
     gene            13093..>14013
                     /locus_tag="WIE35_000534"
     CDS             13093..>14013
                     /locus_tag="WIE35_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ4153497.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKI"
BASE COUNT         3570 a         3714 c         3659 g         3070 t
ORIGIN      
        1 gaacggcaga cagatgaatt tgcccaagcg gcggcaaggg cggcaatccg ttttttggaa
       61 cactatgcgg gttccggcga cgaagtgctt gcaaactgca ccgaacgcct gtttcaggca
      121 ttgcaaaacg gtcattcgtt tatccgtttg agcggtggcg aggctgacgc gctgtcggca
      181 ctcgcacccg ttgtcggaac atccgccgcg cctttgattt tggaaggcag aaggctgttt
      241 ttgggcagga tgtggcagtt ggaatacgat ttggctgccg agataaaacg cttggcggca
      301 gccgacacgt ccgcccccga cgcggcaggc gcgaggcaaa acctcgcaaa atggtttcaa
      361 ggcacgggca gcgaagggca gcgcgatgcc gccgccttgg cactgttgca gtcttttatg
      421 gtgattaccg gcgggccggg aacgggcaaa acgacaacgg ttgccaaact gctggcgctg
      481 atttgcggtg aagacgaaaa tcttcccaac atcgcgcttg ccgcaccgac gggcaaagcg
      541 gcggcacata tggcgcgcgc gctgcaccgt gcaatcaacg gttttgacgc gccggaggcc
      601 gtccgccgcc atttgctcaa actggaaggg cagaccgtcc accgactgct gaagctgcgc
      661 ccgcccaaaa tgcaggcggc gttcaaccct gtttacccgc tgccgtttga cgtattggtt
      721 atcgatgaag cctctatgct ggatacggca ttgatgctgc aacttttaaa agcggtcaaa
      781 accggcgcgc gcgtgattct gctgggggat gaaaaccagc tcccgtccgt cggaataggg
      841 gcggtgctgt ccgttttgtc acaaaaaacc gttttggacg gagaaacgca ccaaaggctg
      901 gccggcttcc ttccggaaca cggtttcagc gtcagcgcaa atccacccgt gttggcgcaa
      961 aacaccgccc atctgtcgtt cagccaccgc ttcggcgaca acagcggcat cggctgcctt
     1021 gcccgtgccg ccgtatcggg cgatgaaggg gcgtgggcat tgtttgaccg gtttccggac
     1081 gaactggaac attcggaatg cagtccgaac gctcgagtcg aaaggttgta ccgggcacac
     1141 aaagcctatt ggcaggcggt aaaagacggc aatatcgaag ccgcatacgc gggcatttcg
     1201 gatatcgtgg ttctggcagc ttggcggcag gatgcggaag acttcaacga agcctattgc
     1261 cgccatgtac gccgcaaaat gaacataccg gaacatttgg catattttgc cggagagccg
     1321 attatgatca ggcagaacga ctacgcgctt gaactgttca acggcgacat cggactgatt
     1381 atggaagatg tcggacggca gggcagcctt gccgcctatt ttgccgatgc ggacggattt
     1441 aaaaaggtag cggtaagctg cctgcccgaa tttgaacccg cattcgccat gaccgtccac
     1501 aaaagccaag gttcggaata ccgggaagta tggctgctgc cgccttccgc cgcaccttcg
     1561 gacgaagggg acgatgcatt gtccggattg agtaaggagc tgttatatac cgccattacc
     1621 cgcgcgagag agaagttcgt attcttcggc ggggaagaag ccttccggca agctgccgcc
     1681 accgtcaaaa cgcgtcagac ggcattgggc agtatgctcg agcgggtatt ttcacaagaa
     1741 taatccgccc gaatgatgcc gtgccggcgg cgcgggcgtt ccgatgcctg ccgccggaag
     1801 cgcaccgccg ccccttatgc ctttttcaaa cggtacagga aagtagtttc cctgtttcgg
     1861 gcaaaagcca gcggattgtt cggatcggca gcttttttgt gaaccggttt ggttttcaaa
     1921 acatccgcaa cgcgcaaacc cgcctgagca aatgccttat ccagaaaatc ggacggacgc
     1981 aggtgcagat gttcggcaag atcggaaatg atcaggcgga tttctccgtc gggattcaga
     2041 tgtttcggcg catcccgcaa aaacgcagcc agcatcgcag attcggggtc gtataacgcg
     2101 gattcgacgg cggaagtcgg cttggcggga agccagggtg gattgcagac aatcagatcg
     2161 gcaaaccctt cgggaaacag atcggtttcc cgtatctcaa cctgtttttc aaagcccaaa
     2221 cgggcaatat tggcacgggc gcaggcgacg gctttcggat tggtatccgt gccgatgacg
     2281 gaaggaatgc cctgtttcgc caaaatggcg gcaagcacgc ccgagcccgt cccgatatcg
     2341 aatgccgtct gaaaacccgt tgacggcgca tgggcgagca ggtcgaggta ttcgccgcgc
     2401 aacggcgaga atacgccgaa aggaacgtgt atgctgccgc ccagctgcgg aacggcaacc
     2461 cctttcttat gccactcgtg cgcacccata aaccccagca gcagattgag cggcaggaaa
     2521 aacggtttgc cgtccgcctc tccgtacacg tcgagcaggg cggcgcgtat atcgggcgcg
     2581 cgtttgttgt ccaacacaaa accggggcgg atttcaacgg caagcatatt cagaatacgg
     2641 ctctgctgcg cctgcttcat acggtgggca tggaaaaggg cggcgatatc cgcatcggaa
     2701 cggacggcgg caggtttgcg agccctcttc ttcattgcag aaagcacctg tttggcattg
     2761 tggaaatcgc cctgccagac agttgcaata ttttgatagg cagccttcaa aatgccgtct
     2821 gcaccgcttt cgcggacata atgccaacct ttgggcggct tttgcacact ttcgttgcgc
     2881 cattcggccc cgttatcggg aaaaataaaa gaagacatgg gatacctgcg tcatgttttg
     2941 aaaatagggc ggcagaaccg taaaccatac ggatggtaca gcaaggagcg gcaacacaga
     3001 acagtttttt gttcccgcct tgtctttcca agcccatgca gtctgaagcc ggaatgtttc
     3061 agacggcatc gcatcaaact ccataaataa accacatatg cttgaaataa taccttcaac
     3121 cccaatgtac gcgaaaatcg gcaatctgtc agacacaaga gagtacctat gacacaaaaa
     3181 gaaaagcatt ttgaggaata tgccgccttg gcaacccttc ctttgcggga tgtcgtcgtt
     3241 tacccgcata tggttctgcc gctgtttgtc ggcaggccga aatccatcgc cgcactggaa
     3301 aacgccatta cccgcgagga accggttttc ctattggcac aaaccgatgc ggcgctagaa
     3361 gaaccggttg ccgccgacct gtatcagacc ggtaccgtcg cacaagtctt acaggtattg
     3421 aaactgccgg acggcacggt aaaagtattg gtcgaaggtc tgtatcgcgg acgtgttctg
     3481 accatcgaag acacgggcgg tctgttcgtt tcccatatag aggcggtcgt ggaagaagac
     3541 acgggcggca ataccgacct cgaagccgtg cgccgcaccc tgttggcgca gtttgaacaa
     3601 tacgccaaac tcaataaaaa aatccccgcc gaaattatcg gcagcatcaa cggcattgcc
     3661 gaaaacagcc ggctaaccga tacggtcgca gcgcatttgc agttgaaact ggcgcaacgc
     3721 caacagattt tggaaattcc cgaaatcggc aaacggatgg aattcctgct ggcacagctg
     3781 gaatccgaac tcgacattat gcaggccgaa aaacgcatac gcggacgcgt caaacgccaa
     3841 atggaaaaat cccagcgcga atattatctg aacgaacaga ttaaagcgat acacaaagaa
     3901 ttgggcgaag aagacgaaaa cggcgaactg gatgccttgg aagcaggcat caaaaaggcg
     3961 gggatgagca aagaagcgga agaaaaatgc ctgtccgaac tgaaaaaact caaaatgatg
     4021 ccgccgatgt ctgccgaatc caccgtcgta cgcaactaca tcgacaccct gctcgaactg
     4081 ccgtggaaga aaaaatcccg cgtcagcaaa gacatcgcca aagccggact ggtgctggat
     4141 gccgaccact acggcctgga aaaagtcaaa gaacggattt tggaatacct cgccgtccaa
     4201 aaacgtatgg acaaactcaa aggcccgatt ctgtgcctgg tcggtcctcc gggcgtgggc
     4261 aaaacctctt tgggcgaatc catcgccaaa gcaacggggc ggaaatatgt ccgcatggct
     4321 ttgggcggcg tgcgcgacga aagcgaaatc cggggacacc gccgcaccta tatcggctct
     4381 atgcctggta agattctgca gaatatggca aaagccggcg taaaaaaccc attgttcctg
     4441 ctcgacgaaa tcgacaaatt gggtagcgac ttccggggcg atcccgccag cgcgttgctc
     4501 gaagtgcttg atcccgagca aaacaacaag ttcgccgatc attatgccga agtggattac
     4561 gatttgagcg atgtgatgtt tattgcgact tcaaacagcc tgaatatccc gaccccgctg
     4621 ctcgaccgta tggaaatcat ccgtctgtcc ggctacaccg aagacgaaaa aatcaatatc
     4681 gcgatgcagt acctcgtgcc gaagcaaatg aagcgcaacg gcgtgaaaga aggggaattg
     4741 gcaatcgaag aaagcgcggt gcgcgatatt atccgttact acacccgcga ggcgggcgtg
     4801 cgttcgctcg accgcgagat tgccaaaatc tgccgcaagg tggtgatgca gattaccttg
     4861 gacgaagata agaagaggtt gtctgaaacc aagaaaacca gcaaagccaa acctaaagcg
     4921 gttaaagtaa atgagaaaaa tctgcacgac tatttgggtg tgcgccgctt cgattacggc
     4981 gttgccgaaa gcgaaaaccg tatcggacaa gtaaccggtc tggcgtggac agaagtcggc
     5041 ggcgaactgc tgaccgtcga agccgcagca ttgccgggta agggcgtgat tcagtgcacc
     5101 ggccagttgg gcgatgtgat gaaggaatcc gtgtctgcag cgtggtcggt tgtccgctcc
     5161 cgcgcggaat cagtgggctt ggctcctgat ttttacgaga aaaaagacat ccatgtccat
     5221 gttcccgaag gtgcgacgcc gaaagacggt ccgagtgcgg gcattgccat gactttggcg
     5281 atggtgtctg cattcaccaa aatcccggtg cgcgccgatg ttgccatgac gggcgaaatt
     5341 accctgcgcg gcgaagtcct gccgattggc ggtctgaagg aaaaactgtt ggctgcgctt
     5401 cgcggcggca tcaaacacgt tttaattcca aaagacaacc tcaaagactt ggaagaaatc
     5461 cctgaaaacg tgaaaaccgg cctgaccatc catccggtca aatggataga cgaggtattg
     5521 gctctgggtt tggaaagcca gcctgagcct tgggcagaac cttctggtgc ggaagcggcg
     5581 gcggaatccg cttcaaaacc aaaaccccgc agcagggcaa ccaaacattg aaacgcagga
     5641 aatgtgttgt aaaaatgcgg tttcgtcctg aaagcctgtc aaatagggtg attccgtatt
     5701 ttttgcttga cacggcaatt tcagaattgc tataaagcga aagttgctca agcagtacaa
     5761 accccgagta ttcgggcatt tcatcatttt ttaagacagg aagggactga ttgtgaacaa
     5821 gtctgaattg atcgaagcga ttgctcaaga agccgacatt tccaaagccg ccgcacaaaa
     5881 agctttggat gccactacca atgcagtaac caccgccctg aaacaaggcg acaccgttac
     5941 tttggtcggt ttcggtactt tctacgtggg cgaacgtgcg gaacgccaag gccgcaaccc
     6001 caaaaccggc gagcctctga ccattgccgc cgccaaaacg cctaaattcc gcgccggcaa
     6061 agctttgaaa gacgcactgt aagccgtttt ttatgaaaaa agccgattct ttaaagaatc
     6121 ggctttttta tcggtccaca ttattctgat ttcaaatcgg caacacactg cttgtcacgt
     6181 gcttcaaagg catttgcgcc gccgagcagg tcaagctgtt cttgtgcgcc gagtttgccg
     6241 aaggatctaa tctgtttctc gctcaatctg tccaaaggct gctcccacat acatttgcag
     6301 tagtcgacgg taaggcgggt attgtttgaa tctaaaccgc gggcccgtaa atcgttttgc
     6361 catttttcgg caaacggaat attcttcacg caagactcga caattttctg ttttgcctgc
     6421 ggtttggaca tcgcgcattg ggagagcagg gcggttgaag cgagcaacgc caaaatgacc
     6481 catgcccaaa tgcggatggt gcggattttg gcttttgctt ttttgcgcgc ggcaacctgc
     6541 tcttccgtcg gcatttcgtg tttcggctca gtcatgcagg ctttccatgc ggatcatggt
     6601 aatcggtttt tccacgcaat ccagtgcttc gatggctgcg attgccgact tgatgtgttt
     6661 ttcgaccgtg ctgtgggtca gaatcacgat ttcggcagtg gtctgatcaa tcacgccttt
     6721 ttgaatcagt gcttcgatgg acacgttttc ttgtgccaac agcgcggcga tttgccccag
     6781 cgtgcccggt tcgtctttgg cttggacgcg caggtagtag ctgctggtaa tttcgtccat
     6841 aggcaggatg gtttgcgctt ggacttgcgc gggttggaac gccagatgcg gtacgcggtg
     6901 ggcggtatcg gcttcaacca ggcgggcgat gtcgatgata tcggcaacca cggcggaagc
     6961 ggtcggcaat gcgcccgcgc ccgcgccgta atataaggtt tcgccaacca tatcggcgtt
     7021 gacgcgcacg gcgttcatca cgccgttgac gtttgccaag aggcggcttt cgggaatcag
     7081 ggtagggtgg acgcgcagct cgatgccttt gccggttttg cgggtgatgc ccaacagttt
     7141 gatgcggtag ccaagttctt cggcgtattt gatgtcgcgg ctgtcgagtt tgctgatgcc
     7201 ttcgaggtag caggcggaaa agttcatcgg cgtgccgaat gccagcgcgc tcatgatggt
     7261 gattttatgg cccgcgtcat gtccttcgat gtcgaaggtc gggtcggctt cggcatagcc
     7321 cagtgcctgc gcttctttca gtacatcggc aaacgcgctg cctttttcac gcatttcgga
     7381 gaggatgaag ttgctggtgc cgttaataat gccggcgatg gatttaatcc tgtttgccgc
     7441 caaaccttcg cgcagggctt tgatgattgg gataccgccc gctactgccg cttcaaattg
     7501 caccatgaca tttttttctt ccgccagcgg gaagatttcg ttgccgtatt cggcgagcag
     7561 ttttttgttg gcggtaacga tgtgtttgcc gttttcaatg gctttcaaca ccgcatcttt
     7621 ggcaatgccc gtgccgccga acaattcgac gacgacatcg acgtctttac gcgcgaccag
     7681 ttcgaacgga tctttgacaa aggctgcgga cgggcagatt tggcgggctt tttcttcgct
     7741 caagtcgcat acggcagaaa tacggatttc gcgccccaag cgacgggaaa tttcctccgc
     7801 gttgtcccgc aacacggcag ccgtaccgcc gccgaccgta cccaaaccta aaagaccgat
     7861 gtttactggc ttcattgtgt ctccttgtaa gccgactgaa atgtaaatat tgaaagacga
     7921 aaatatccgc tgccgatata atggtgccgc actttgaatc aaatgccgtc tgaaatcggc
     7981 aggcgagtca gatgaaatct gccaatccta catgaatttg tctgattttg cacccctttc
     8041 ggtgtagatg atgcggcgac ggggtaaaaa aatgttgttt gaagaaaatc cgatagacgg
     8101 acagtttgcg gaatatgaat gcggtgcggg cggaatccgg ctggcggggc aaagtttcca
     8161 taaacccgtg cttgtacata aggattcggt ctgcctgtcg caatgccgaa ccttgtccga
     8221 tctgactccg gaaaacctgt tgtccgacat caaacctgtt gactatccgg aaatattgat
     8281 tatcgggacg ggcgcggctc aggagtttat ccatcccaaa atcatggcgg atttttcccg
     8341 aatcggaatc agcgtggaat gcatgaatac cgattcggca ttcaggacat tggttttcct
     8401 gcactcggaa gggcgcaggg cttgggcttg gcttcagccg taaatttccc gtttcagacg
     8461 gcatcggcac tgactttcag gtaaaatacg ggcttttccc gcccgacgat gtttccgtta
     8521 tgattgaaat caaaaacctc accctgcaac gcggtttgaa agtcctgctc gacaaagcca
     8581 acgctaccgt caatcccggt cagcgcgtcg gtttgatcgg caaaaacgga acgggcaaat
     8641 cgagcctgtt tgccttaatc aagggtgaaa tcactcagga cggcggcgat gtctcgattc
     8701 cgaaaaactg gcggctcgct tccgtttccc aagaaacgcc cgatttggat atttccgctt
     8761 tggattacgt tttgcagggc gatgccgagt tgcaggcttt tcagacggca ttgaggcagg
     8821 cagaagcgca aaatgacggc atgaagcagg cggaatatca tgccaaattg gaagaaatcg
     8881 acgcttatac cgcgccggcc cgcgcggcaa aattgttgaa cgggctgggt ttttcgcaag
     8941 aagaacacag ccgccccgtc aaatcctttt ccggcggctg gcgtatgcgc ctcaatctcg
     9001 cccaagccct gatttgccgc gccgatttgc tgcttttgga cgaaccgacc aaccacttgg
     9061 atttggaaac cgtcttgtgg ctggaaaacc atcttgcttc tttaccctgc acgcaaatca
     9121 tcatttccca cgaccgcgat tttctcaacg cggcaactac ccaaaccatt gaattatcgc
     9181 agcaaaaatt cacgcaatac ggcggcaatt acgattttta ccaaaacgaa cgcgcgcagc
     9241 gtctcgcgca acaacaagct gcctatgtca aacagcaggc gcaaatcaaa catttgcaat
     9301 cctttatcga ccgcttcaaa gccaaagcca ccaaagccgt tcaagcgcaa agccgcatga
     9361 aggctttggc aaagctagaa cgcgttgcgc ccgcgcatct ggacagcgag ttttcctttg
     9421 agttttacca tcccgaccat ctgcccaatc ctttgttaaa gctagaacac gctgatttgg
     9481 gttacgaagg caaaactgtc ctacacgaca ttaccctgtc gctggaaagc ggcgcacgct
     9541 acgggctatt gggtgtcaac ggcagcggca aatccacctt tatcaaagcc ttggcaggca
     9601 aaatcgattt gctttccggc agcatcatcc gttccgaaaa actcaatatc ggctattttg
     9661 cccaacacca actcgacacc atctgcgccg accaaaaccc tgtttggcat attcagcagc
     9721 tttctcccga agtacgcgaa caagaaatcc gaaatttcct cggcggcttc aattttgttg
     9781 gcgatatggc gttacagaaa accgaacctt tttccggcgg agaaaaagcc cgccttgccc
     9841 ttgccatgat tatctggcaa aagccgaacc tgttgctgct tgacgaaccg accaaccatt
     9901 tggacttgga tatgcgccac gccttaacgc tcgcgttgca aagtttccaa ggcgcattaa
     9961 tcgccgtatc gcacgaccgc agcctgcttg aagcgactac cgacagcttc ctcctgatcg
    10021 acaaaggccg tctgaagaac ttcgacggcg atttgaacga ttaccgccaa tggcgtttgg
    10081 cacaggaaaa cgccgccgcc gcacccgcag catccgcaca aagccaaagc cgcaaagaca
    10141 ccaagcgcat cgaagcgcaa atccgtcagg aaaaagcccg acgcggcaag ccgatacagc
    10201 agaaaataga ccgtgccgaa aaagaaatgg cgcagctttc cgaaattcag acggcatgtg
    10261 aagcattttt agcacaagaa gacgcttacc ttgaagcaaa caaagaaaaa ttgcaaaata
    10321 ccttatccct gctggcaaaa gtcaaaacac aacttgccca aatcgaagag gtttggctgg
    10381 cttgtcaaga agaattggaa cggattgaaa ctgaaatcga gaaacagttt gccgagcgat
    10441 aaagaagcgc ggacagggtg cttatctttg cctgccaata acggtataat tcgggcgtta
    10501 tcaccgcctt ttaccggtat aaacatcaga ctttttgccg ccttgggtct cctgtccctt
    10561 tccggcgcgg cggcgcaagc ctccgtatac cattgcaact caaatggcaa aagcgtatat
    10621 acatcggccc cagcaggatc gtgcgccgat gcggatttac ctaaaatcag cagccatcag
    10681 ggaggcggat accgcctgaa aattaaaaaa ctcagttaag aagccaaaat acacatagaa
    10741 agtaaaaaga aaaacaaaaa acctgccggg aaaaagaaca agcaggctgc caaagccccg
    10801 aaagaaaatc aaaagcaaac aaccgaaaag aaaagcccgt aaaacgccaa gaaagcatta
    10861 caaaaaatac caaaaaatca aataattatc cgagcatcaa gcacattatg aaatcaaaac
    10921 tccccttaat cctaatcaac ttttccctga tttcaagccc attgggtgcg aatgcggcca
    10981 aaatctacac ctgcacaatc aacggagaaa ccgtttacac caccaagccg tccaaaagct
    11041 gcctctcaac cgatttgccc ccaatcggca actacagcag cgaacgctat atcccgcccc
    11101 aaacatccga accgacacca tcaccgtcaa acggcggaca ggctgtcaaa tataaagccc
    11161 cggtcaaaac agtatccaag ccggcaaaat ccaatacgcc cccgcctcaa caagcacctg
    11221 taaacaacag cagacgctcc attctcgaag cagaattgag caacgaacgc aaagccctga
    11281 ctgaagccca aaaaatgtta tcacaagcac gtctggcaaa gggcggcaac atcaaccatc
    11341 aagaaataaa tgcattacaa agcaatgtat tggacaggca gcaaaatatc caagcactgc
    11401 aaagagaatt gggacgtatg taaagccgtg ttttcaaatc gaccgttcca aggatttgac
    11461 agaagaaatg atgaaaaagc agcggcatat cctgtgtgtc ggctgcctgt atattatatt
    11521 gaaggtcaac gtatttccca ataccggctg gatggaagca tcgaaaggga tgcagggcat
    11581 atcgttcagt accttcggat acgcggatgc tccgggctat acattcagcc gcattgtcca
    11641 tactttaatt ttcctacttg gaaagaccac agggacacgt acaactgttt ccgatagttc
    11701 gcataatgta tattatgtta aattattgcc aaagccctca accaaacgac tttgtcggat
    11761 ttgtcggcag gcgaacaaaa gttgtttttg gaaatggcgg caaaaaatgc cgtctgaacc
    11821 ctaggattca gacggttttg cattatgggc cggttttcct taatcacttt gtttccggtt
    11881 tcaaaagcag ccttcagtgg ggtttaagca acttggcatt cagatggcat tcacagcttc
    11941 cagtttatcg gatcgatacc gtgccggctc aaatagctgt ttgcctgtga aaaatggcgg
    12001 cagccgaaaa aaccgcgata tgccgacaga ggagacggat gcggtgcggt caatatcaaa
    12061 tggttttgac tgtctatcag tttccttttt tgttgtgcat acccacccca cagcataaag
    12121 acaagatgct tgcggtgtgt cgcaagctgc ctgatgacgg tatcggtaaa gcgttcccag
    12181 cctaaaaggg cgtgcgaatg cgcctgtcct gcacgcaccg tcaaaaccgt gttcagaagt
    12241 aatacgccct gctccgccca cgctgtcagg cagccgtgcg cgggtatgga aaagccttcg
    12301 atgtcggttt ccaattcttt gtagatattg agtaaagacg gcggtatgcg gataccctgc
    12361 cggacggaaa atgccaaacc gtgcgcctgc cctgccccgt gatacggatc ttgtccgaga
    12421 atgacggctt tgacccggtc gaacgctgtc aggcggaatg cgttgaacac atccgccgcc
    12481 ggcggataga tgatttgtcc cgacaaacgt tcctgcctga ctgcatttaa aatttcctga
    12541 aaatacggct gctgtttttc gccgccgagt gcatcgtgcc aagtgtccat atctgcccta
    12601 tcctttcaaa ttcggctata ctgctgatat tgcgttattt tgtcaaacga cgcgctttgt
    12661 ggggaaatgt atgactgtat ggtttgttgc cgctgttgcc gtcttaatca tcgaattatt
    12721 gacgggaacg gtttatcttt tggttgtcag cgcggctttg gcgggttcgg gcattgctta
    12781 cgggctgacc ggcagcacgc ctgccgccgt cttgaccgcc gctctgcttt ccgcgctggg
    12841 tatttggttc gtacacgcca aaaccgccgt tagaaaagtt gaaacggatt catatcagga
    12901 tttggatgcc ggacaatatg tcgaaatcct ccgacacaca ggcggcaacc gttacgaagt
    12961 tttttatcgc ggtacgcact ggcaggcgca aaatacgggg caagaagagc ttgaaccagg
    13021 aacgcgcgcc ctaatcgtcc gcaaggaagg caaccttctt atcatcgcaa aaccttaaca
    13081 ctcggaggaa ttatggaatt tttcattatc ttgttggcag ccgttgccgt tttcggtttc
    13141 aaatcctttg ttgtcatccc acaacaggaa gtccacgttg tcgaaaggct cgggcgtttc
    13201 catcgcgccc tgacggccgg tttgaatatt ttgattccct ttatcgaccg cgttgcctac
    13261 cgccattcgc tgaaagaaat ccctttagac gtacccagcc aggtctgcat cacgcgcgac
    13321 aatacgcagc tgactgttga cggcatcatc tatttccaag taaccgaccc caaactcgcc
    13381 tcatacggtt cgagcaacta catcatggca attacccagc ttgcccaaac gacgctgcgt
    13441 tccgttatcg ggcgtatgga gttggacaaa acgtttgaag aacgcgacga aatcaacagt
    13501 actgttgttg cggctttgga cgaggcggcc ggggcttggg gtgtgaaggt tttgcgttat
    13561 gagattaaag acttggtgcc gcctcaagaa atccttcgct caatgcaggc gcaaattacc
    13621 gccgaacgcg aaaaacgcgc ccgtatcgcc gaatccgaag gccgtaaaat cgaacaaatc
    13681 aaccttgcca gcggtcagcg cgaagccgaa atccaacaat ccgaaggcga ggctcaggct
    13741 gcggtcaatg cgtcaaatgc cgagaaaatc gcccgcatca accgcgccaa aggtgaagcg
    13801 gaatccttgc gccttgttgc cgaagccaat gccgaagcca tccgtcaaat tgccgccgcc
    13861 cttcaaaccc aaggcggtgc ggatgcggtc aacctgaaga ttgcggaaca atacgtcgcc
    13921 gcgttcaaca atcttgccaa agaaagcaat acgctgatta tgcccgccaa tgttgccgac
    13981 atcggcagcc tgatttctgc cggtatgaaa att
//
LOCUS       DAWHXZ010000027        13857 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000027 DAWHXZ010000000
VERSION     DAWHXZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13857
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.027"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..745)
                     /gene="tsaD"
                     /locus_tag="WIE35_000535"
     CDS             complement(<1..745)
                     /gene="tsaD"
                     /locus_tag="WIE35_000535"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ4153498.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPIHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQD"
     gene            complement(862..2049)
                     /gene="ccsB"
                     /locus_tag="WIE35_000536"
     CDS             complement(862..2049)
                     /gene="ccsB"
                     /locus_tag="WIE35_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ4153499.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRTEGKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(2042..3994)
                     /locus_tag="WIE35_000537"
     CDS             complement(2042..3994)
                     /locus_tag="WIE35_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ4153500.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGSFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(4271..4894)
                     /locus_tag="WIE35_000538"
     CDS             complement(4271..4894)
                     /locus_tag="WIE35_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ4153501.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            5100..5729
                     /locus_tag="WIE35_000539"
     CDS             5100..5729
                     /locus_tag="WIE35_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ4153502.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(5850..8246)
                     /locus_tag="WIE35_000540"
     CDS             complement(5850..8246)
                     /locus_tag="WIE35_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ4153503.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            8398..9513
                     /locus_tag="WIE35_000541"
     CDS             8398..9513
                     /locus_tag="WIE35_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ4153504.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKLLYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            9516..10115
                     /locus_tag="WIE35_000542"
     CDS             9516..10115
                     /locus_tag="WIE35_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ4153505.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            10116..10763
                     /locus_tag="WIE35_000543"
     CDS             10116..10763
                     /locus_tag="WIE35_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ4153506.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            10781..11326
                     /locus_tag="WIE35_000544"
     CDS             10781..11326
                     /locus_tag="WIE35_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ4153507.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            11345..13630
                     /locus_tag="WIE35_000545"
     CDS             11345..13630
                     /locus_tag="WIE35_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ4153508.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAATPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGGDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNAELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
BASE COUNT         3869 a         3979 c         3035 g         2974 t
ORIGIN      
        1 catcttgaaa cgcacggcag atgtcgttgc gtgtctgctc aggaatgtca tccgccccgt
       61 tttccgcgcg cactttctcg acggcagtca aaacagcggt tttcaaacct gaaaaactca
      121 tctgcaaatc gtcggaatga atcatcgggc gcggaaaaac aaacgcttcg ggcctgcccg
      181 attccgcaag ttccgacagt ttcgcaccgc ccggatacgg caagcccagc agtttcgccg
      241 ttttgtcgaa tgcctcgccc gccgcatcat cgacgctctc gcccaaaagc gcgtagtcgc
      301 ctatgcccct gaccgccata atctgcgtat gcccgcccga aaccagcagc gcgacaaaag
      361 gaaagtcggg tttttcctcc gccaacagcg gcgacagcag atgtccttcc aaatgatgga
      421 tggggatgac gggcttgccc aatgccaaag ccagtgcatt ggcgtagctc gaacccgcca
      481 gcagcgcacc gcccagaccg ggtccctgcg taaaggcaac cgcgtcaatg tcgccatacg
      541 atgcacccgc ctgcgccaaa cagccttccg tcaacggaac aaggcggcgg atatggtcgc
      601 ggcttgccaa ttccggcaca accccaccgt attcggcgtg cattgccatt tgagtgtgca
      661 ggcagtgcgc ccgcaatcct cgttccgtat cgtaaagcgc aacgccggtt tcgtcgcaag
      721 aagactcgat tcctaatacc aacatggtct gatgccgtta aaaactgaaa aacgtatttt
      781 agcggatttc ggcacgactg ccgtatccca aaaacggaac atgccgtctg aagaccgttc
      841 agacggcatc gtcgcaccgt atcaaagcgt tccgtaagaa tgcagcccgc tcaaaaacat
      901 attcacgccg ataaaggcaa atgcggttac gaacaaaccg ataatcgccc accacgccag
      961 cactttgccg cgccaaccgg caaccagccg caagtgcagc caaacggcgt aattgagcca
     1021 gacgatgaac gcccacgtct ctttcgggtc ccaactccaa tagcgtcccc aagcatccgc
     1081 cgcccacagc gcacccaaaa tggtggcaat ggtaaagaac agaaagccga cggcaatcgc
     1141 cttatacata acttcctcaa tcaacacgga ttgcggcagc cacgatttcc cgccttttcc
     1201 ttccgtacgc agggaaacca gttcggcaat accgagcatc gcggaaatgc aaaacgcgcc
     1261 gtaaccgata aagtttgccg gaacgtggat tttcatccac caagattgca atgccggaat
     1321 cagcggctgg atggtatgcg cctcgcggga cacgctgtac cacaagacaa atccaaccac
     1381 gaccgccata aagccgaaca cgaagccgcc caatttctgt atggcgaact taccttcata
     1441 ataaagatac atcagcgcgg taatcaccaa aaacaggatg aacacttcat acaggttgga
     1501 aaccggaata tgccccgcat cgggacggag cagatagctt tcgtgccaac gtaccagcag
     1561 accggtaaag cctgctacgg cagacaccca tgcaaacacg gttcccatac ccaacagcgt
     1621 gttggtcggc acatttttta cgcttgccaa aaccgcgccc gaaatatagg cgaacagggc
     1681 gaagaagaca aaggcgcact gccacataat cgccgactgg ctgctgagga aataccgcaa
     1741 caggaaaatc tctgccgatt tgatgttgcc gccatacaaa ccgacggcgg cataggcaag
     1801 caatacgctt aaaggaacaa accagcgcat cggcttgaaa aaccagccca aaaatacggc
     1861 aataccggca ctcgcccaca acatgaccgt ttcgtaaatg tccatatgca taccggaacg
     1921 ggtctgtacg aaaaccgtag ccgcaaaaac cagcacggca aatacccaat cccaaagatt
     1981 cagattgctg atcaaagact tctgaatcag cagctcgtgt tccggaaggg ttttatagtg
     2041 ttcagtcatg attcaagtcc ttgccgagcc gttgcagact ctcgacgtgt tttggaaatt
     2101 ccttctgcaa atcccgttcg ctgcgggccg aagacatggc aaaacggatt ttgccgtctg
     2161 aaaacaatac ccacgcccgt ttttcgcgca cataaaacat caataccgta cccaatacca
     2221 acagcaccga gccgagatag accaaaagcg cacccgggga acgggtcatc tgcaaacccg
     2281 acgaacgcac ctcggaaaac ccatcaagtt gcagcagcat aggcgcggga tattcggtca
     2341 aacccgtgta cgcatccata ctgtgcagca ggaaacggtt ccgcgcttca tcctgctgcc
     2401 attcgggcaa gccgtaccgg cgtatggttt catccaaagc agcgttcatc acgccgtaaa
     2461 gcatttcgta gaaataaccc tgcatcttat cctgctgctc tttcggaata ttggacgtaa
     2521 taaattcgtc caatcccaaa tagccttttt gtgcaaagat gttcagcgtg ttttccgcag
     2581 ccagcatgaa ttgttcgcgg atttcggcag gtgcgtcttt ggttgcgtcg gcaaccagac
     2641 gtttgcgccc ttccccatct ttcaaaaact cgcgcaatgc cataaaggtg tccgctttca
     2701 actgcttgtc caaggggata cgcagccagc ggtattgctg ctgcaagccg ctgcgcgtgc
     2761 cggtaatcca aaaataatcc tgttcctgca aaaccggcag catatagttt ttatattcga
     2821 ccgcctgccc tgccgcatca cggatacggt aaacaatgga agggccgata ttggtgtatt
     2881 ttttaccttc ctgagtaacg gcgcggacat cgttcagcgt ggatttcagg cttttttccc
     2941 gttccgcgcc ctcgctcatg tcctccacat tcatagaagt gaactgatcg aactcaagac
     3001 gatatttgtg tttgccaatt tccaacggaa actggtgtat ggatgttgcc ttcaacacga
     3061 caggctcgcg cgaagcatca cccaaattcc acgccttgaa tgtcaaatcc gaaccgccgt
     3121 cggcaaaact cgcctgataa atcgtgatgc cgtgcaaggt caaaggatgg ttcacgcgga
     3181 tggtgcgctc gagtttctca ccggttgcct tatccgttac ttcaatatca ctggcaaaat
     3241 cgcgcggcat acccgtattg taaaaatcga tatggaattt tttcagtttg acttcaaaag
     3301 gcaagtcctg aaccaatatc ccgttgtcgg cattcaggaa aaccacatcc gcactctgcc
     3361 cctcggaaat attgacgttg cccctaaatg agagattgga cgcacccaaa atactttcgg
     3421 gcttgaaatc cttggcataa accgcctgat tgtccggaac aatccgaccg gtcagcatac
     3481 ccagtttcaa cagcaggtta ctgtctatca acccgcccag gcaaatgaca atcaaagcaa
     3541 catgggcaaa gatatagccc catttgttca ttgtgccttt tttggcggca atcagaaccg
     3601 acccgtcttc acggttaatg gtttttccct gaaaaccttg tacttccaga taacgtttgg
     3661 caacctcggg cgcaattttt acatccaaca gcgaagaatg gcgcatcgcc gccagagatt
     3721 tttctttaac cttttcccga aaagacttca tttcgcgcca gaacggcggc acattgcgaa
     3781 tcaggcacaa actggtagaa accaccaaaa acatcatgat aacgacaaac catgccgaag
     3841 catagacgtc atacagtccc agaaaaccaa aaatctgcgc ccaaaacgat ccgaatttga
     3901 ccaaataatc cgtctgcggc tggttttgct gcaacaccgt accgataacc gatgcaatac
     3961 ccagcagact gagcaaagcg actgcaaagc gcatggagct gaaaaaagcg aaccacggac
     4021 gggaaagaag tgggggagat ctacgggatt tactcattgt gtgtttattc cgccatcaga
     4081 aatatgggaa agcagaattg ggcaaacaga aaacaacgtc ccgattctac tgtcttgatg
     4141 ctttttattt caagacaatg aagacagcct gcatcgattc caacggttgc gattgaaaaa
     4201 acttatcgca gaattgcctg aagccgtctg aaaacttttc agacggcctc taaaacagac
     4261 tattgcggaa ttaacgcaaa ccttggataa agttggcgac cgctttcaaa tcttcttcag
     4321 acatacggtt tgcaatatct tccatgatgg tatttttacg ctgaccggac ttgtaggcat
     4381 tcatctgttc aacaatatat gcctgatgct gaccgcccaa acgcggataa gcctgaattt
     4441 cgcttccgcc tcccggcata cccgcaccgc tcggaccgtg gcaggacata cacgccggca
     4501 cttttttatc gctcaaaccg ccgcgataga ttttcgcacc caattcggga ttttccttag
     4561 gattggcttc accggatttg ggctgctgtt tggcatagaa tgcggatacg ttcaaaatat
     4621 cctgatcgct caaattcatt accaccggtt tcatcacagc tgccgaaccg tgggtgcgtt
     4681 taccgtcgcg gatgccgata gtttgatgat agatgtaagc agtatgctgt gccgccaaac
     4741 gcggatacat cgcaatgccg ctgttaccgt ctgctgcatg gcaagccgca caaaccgttg
     4801 cggcaacctg tttgcctttt tccacgtctg ctttgggaga ggcggaaacc gcaccggcag
     4861 ccaaaacaaa ggccaataaa gtcaatcgtt tcatggagtg ctcctgatta cagcattgga
     4921 taacgcaaca atgctctttt tatattcaaa tacgggattt ttgacccgat taaaaccgat
     4981 gattctgtaa acgtgttatt ctatactaaa tttacattaa attaccactg tgtttcacat
     5041 aaaaccaacc gcatattttt gctgtcggac aaacggcggc ggaaaacaag gatatgccca
     5101 tgaacctttt tcaaaacgcc aaattcttca cgacggtcaa ccaccttaaa gacctgcccg
     5161 acacccctct cgaaattgcc tttgtcggca ggagcaatgc cggaaaatcc agtgccatca
     5221 ataccctgac caaccatgtc cgtcttgcct acgtttcaaa aacacccgga cggacgcagc
     5281 atatcaactt cttcgagctg cagaacggca attttatggt cgatttgccc ggctacggtt
     5341 atgcccaagt ccctgaagca gtacgcgcac attgggtcaa tctgctcggc gactatctgc
     5401 aacagcgcaa acagcttatc gggctggttt tgattatgga tgcccgccat cctttaaaag
     5461 aactcgacat ccgtatgctg gattttttcc acacgaccgg cagaccggtt cacatcctgc
     5521 tgtcaaaagc cgacaaatta tccaaaaacg aacagataaa aaccctgtcc caagtcaaaa
     5581 aactgctcaa accttattcc gacaggcaaa acatcagcgt acagctgttt tccagcctga
     5641 aaaaacaagg tattgacgag gccaaccgaa ctgtcggaag ctggtttgac gcagcagatg
     5701 ccgccgcttc ctctccagag gaaaactgac cccaattata cggaaaccgt atttccccca
     5761 cttgaccgac cgcaaacatt taaaaaattg ccactgccaa atctaaaatg ccgtctgaaa
     5821 gacttttcag acggcatttt gcggagtctt taaaacagag aatccaactg ctgctgtttg
     5881 gaaccagtat tactcggaag caccggcgtt tcctgcatat cttggcggac ttcgtcatcc
     5941 gccgcctgcc gtccgccttc tgccgcgccc ccgtcatctt cttttgcccg tcgggaaggt
     6001 tgcggcgcaa taccgctgtt gtccagcgtc aagcccggat cggttaccat acgttccttc
     6061 atatagtatt cgccattgct gctgaccaca ccttcaggca ttttcatccc cttgccctgc
     6121 tttcctttca acgcaaaacg catatagtcc acccaaaccg gcaccgcaat cgtaccgccg
     6181 tagccgacac gccccatact cttaggtttg tcgaagccga tatatacggc agtaaccaca
     6241 tcagggttaa aaccgacaaa ccacgcatcc ttattgtcat tggtcgtacc cgttttaccg
     6301 gcaatatccg ttcttcccaa cgcagctgcc ccccttgccg taccgacacg gaccacatcc
     6361 tgcataatct tatacataat ataggcattg cgcggatcga ttgcctgagg cgcattttgc
     6421 ccggccacca aaggttgcat ttgggcgcgc aacctgccgt ctctgtcata aatcttatcg
     6481 attacgtgcg aagaaaccct atatccgccg ttcgcaaata cgctatatgc ctccgccact
     6541 ttcaacggcg ttgtctcgcc cgtacctaaa gccatagaca ggcttgccgg cagctcggac
     6601 gacctgaagc cgaaacgccg gatatactgt tgcgcgtaac cgacaccgat agacatcaaa
     6661 atacggatgg aaaccatatt cttggaagcc gtcagagcct gtctcaaagt aatgtagccg
     6721 gaatatctgc cgtctgaatt tttaggtgtc caaaccgaac cgttcggccc tttccccggc
     6781 agggaaatcg gcgcatcgtt aaccaccgtg gacgcggtca tccccttaga taatgccgcc
     6841 gaatagacaa acggcttaaa ggtcgaaccc ggctgccgca ttgcctgaac ggcacgattg
     6901 aatgttttgc tgtgaaaatc ataaccgccg accagcgcgc gcacagctcc ggtttttgca
     6961 tccagcgaaa ccaaagcccc ctgcagcaac ggctcttgaa ccaccgccca acgcccgccg
     7021 ttgtttttga cacggatgac cgcgcccctg cggatacggt cctcccccat tttttcatta
     7081 ttgaccgcgc gggccgcaaa gcccaaggcg cgcctgtcaa gcgtaacccg cctgccgccg
     7141 ggcagctgta tgacgacatt tttcttttta gtcacatcca acacaacggc gggaaccatt
     7201 ttatcgacgg tatagagtcc cgacagatac tggctgacag tctcctcgac atcttcactc
     7261 ttactcaaat cgatatagtt ttccgcaccg cggtagctgc tgccgcgatc gaaattccgt
     7321 agagccttgc gcaatgcctc ggttgccacc ttctgatgat cggcgcggac cgtggtataa
     7381 accttaaaac cctgcgtata ggcatcttca ccgtatttct catacagttc ctgacgcacc
     7441 atttccgcca catataaagc actctgatcg attttccgaa caaaccgctc gtaatgcagt
     7501 tcctcattca acgcctgatc gcgctgttgc acggtaatca tcttctcctc gagcatattg
     7561 ttcaaaatat acttctggcg caacttggca cgttctggat taacaatcgg attataggca
     7621 gacggagcct tgggcagtcc cgcaagcatg gcggcttccg ccaaagtcaa atctcggaca
     7681 ttcttattga aatagatttg cgcggcagat gcaaaaccat aggcgcgctg accgaggtaa
     7741 atctgattga aatacaactc gaggattttg tctttgctta aagactgctc gattttatag
     7801 gcaagcaaca cctcattgaa tttgcgtgtg aacgtttttt cactgctcaa ataaaaattt
     7861 ttcgccacct gctgcgtaat cgtactcgca cccgactgca cgctgccgga cacgacattg
     7921 ccgacggcag cgcgggcaac accccaaaca tccacccccc aatgccggta aaagcgttta
     7981 tcctcggcgg cgataaccgc attccgcaac acctctggga aatcgccgat ttttgtaaat
     8041 tcgcgccgct gctccccata cataccgatg acttccccat ccgccgaata aatagtcaac
     8101 ggcattttag gctggtaatg ctgcaaagaa tccaaagacg gcagtttcgg atacgttacc
     8161 aaaatagcaa tggcaaccaa acccactcca aatacacaaa acccaaaaac caaaccaaaa
     8221 caagtcgtta aaatcttttt aatcatagct gaataataat ttaccattat tggtattaaa
     8281 taaagtaaaa tagcaaccga tttctacaaa gcacggtttc aatgtgcaaa gaacaaggaa
     8341 tccattacgg ataccgaaac ggttactcac tgtacaaata aagcaggaac tttcatcatg
     8401 cgcttgttta aaagcttgaa aaaccctaaa aaaacagatg ccaagctccc taaaaaatct
     8461 tcgggactca ataaccgcgc ggcaatcggc atcgatatcg accagcattc catcaaaatg
     8521 gtccaattgt caggacgtag tttaaaccaa attcaattgg aaaaatacgt cattgccaaa
     8581 ttaccaaaga atatcattca aggcaataaa gtccaaaatt acgatcaact tgttacatat
     8641 ttgcaacaag cctatgccaa actgggtact tcgtgcaaaa acatcatcgc gtccgtcccg
     8701 caaaatctgg caaccatcga acaattgacc tacacagaca aagatgcaga attagacctg
     8761 caggggttcg tggagtcctc catctccgaa gtcagctcga tatcgctcga agaagccaat
     8821 tacgactatc aggtcttgtc ccaatcggcc gccggcgaag ctgtgttggc cgtcgcatcg
     8881 agaaaggatg aaatcgaacc cctgattgac gcattcaacg ccgccggtat gaaattatcc
     8941 gcgcttgatg tggacatttt cggacaatac aacgcctacg cgctatggat aaaccatttc
     9001 gcccccgagc ttgcggccga aaaagtcgcc attttcggcg tatatgccgc acagacctac
     9061 gccttggtca tccaagacgg aaaactccta tacaaacagg aaacctccgt cagcgaagaa
     9121 cagctcaacc aactcatcca gcgcacctat caggtaacag aagaaaaagc ggaagaaatc
     9181 atcaactccc cgcaaaaacc ttccgattac caagaaagcg tggcaaacta tttcaaccag
     9241 cagattaccc aagaaataca aagggtcttg cagttttatt acaccacgca gaccgcagac
     9301 gatatgaccg acatcaagca tatcctgctg accggggaag cggcgcgcca ggaaggcatc
     9361 gcccaaaccg tcgcctcaca aaccaatgca gatgtacaat gcgtccatcc cgcgcgttat
     9421 tttgcggaca acctcaaaac agacaaacaa caattcgaac ttgatgcgcc gacactgacc
     9481 agggcgttcg gtttggcggt acggggatta taattatgaa caatttaatc aaaatcaacc
     9541 tcctccccta cagggaagag atgaacaagc gcaaacagca gcagtttaaa acgctgatgt
     9601 acggtgccgt gctgacgggc gttgccgccg ttgcggcaac ctacctgttt atcgacaata
     9661 tgatcaataa ccagtcggaa agaaacacgc tgctggaaac ctccatcgca cacttggata
     9721 ccgagctgtc ggaaatacaa aagctcaaac aggaaaaaga tgccttcctg attaagaaaa
     9781 acaaaatcga ggagctccag ctcaaacgcc tccaagccgc aaaaatcctc gacagcctga
     9841 atgaggccgt ccccggaagc acctacctga cctcgctgga tgccgttacc gccgactctt
     9901 atcggctcag cggcaggaca tccagcgaca accgcgttgc cgccatgatg agggcgatgc
     9961 ccaataccgg catattcaag caacccgaat tgttaagcat caagaaaaac aattcgcatc
    10021 aagaatttac ccttcaggca acattacaac ccatcgtaaa ggcggccgaa tccaaagaga
    10081 atccggcttc gggaaacgca caggaggcaa actgaatggc ttctaaatca tctaaaacca
    10141 acttggatct caacaacctt cacctgctca accttcctgc caggcttttt atcgccctgc
    10201 tggccgttgc cgccgtgctg gggctcggtt atgccggatt gttcaaaagc cagatggaat
    10261 cccttgagga atacgaagca aaagaaaccg aactgaaaaa cacctacaaa cagaaaagta
    10321 tcgacgcggc cagcctgaac aacctgaggg acgaacttgc ctcaatccgc tctgccttcg
    10381 atatcatgtt gaaacagctg ccgacagatg cagaaattcc caacctggtt caagagcttc
    10441 atcaggcagg ttcgagcaac ggcctgcgct tggacagcgt tatgccccaa cctcccgtag
    10501 atgacggccc catcaaaaga ttaccctatt ccatttccat taccggaaat tacgaacaga
    10561 tcagccaatt tacccgcgat gtcggcagcc tctcccgaat cattaccctt gagtcgctga
    10621 aaatcgccca atctccggaa aacggcggca atcctgacgg caagagcagc atcctgaacc
    10681 tcagtgccat tgccaccacc taccaagcaa aatccgtaga agagcttgcc gcagaagcgg
    10741 cacaaaatgc cgagcaaaaa taacttacgt tagggaaacc atgaaacact atgccttact
    10801 catcagcttt ctggctctct ccgcgtgttc ccaaggttct gaggacctaa acgaatggat
    10861 ggcacaaacg cgacgcgaag ccaaagcaga aatcatacct ttccaagcac ctaccctgcc
    10921 ggttgcgccg gtatacagcc cgccgcagct tacagggccg aacgcattcg acttccgccg
    10981 catggaaacc gacaaaaaag gggaaaatgc ccccgacacc aagcgtatta aagaaacgct
    11041 ggaaaaattc agtttggaaa atatgcgtta tgtcggcatt ttgaagtccg gacagaaagt
    11101 ctccggcttc atcgaggctg aaggttatgt ctacactgtc ggtgtcggca actatttggg
    11161 acaaaactac ggtagaatcg aaagcattac cgacgacagc atcgtcctga acgagctaat
    11221 agaagacagc acgggcaact gggtttcccg taaagcagaa ctgctgttga attcttccga
    11281 caaaaacacc gaacaagcgg cagcacctgc cgcagaacaa aattaagaag aggattactc
    11341 cattatgaat accaaactga caaaaatcat ttccggtctc tttgtcgcaa ccgccgcctt
    11401 tcagacagca tcggcaggaa acattacaga catcaaagtt tcctccctgc ccaacaaaca
    11461 gaaaatcgtc aaagtcagct ttgacaaaga gattgtcaac ccgaccggct tcgtaacctc
    11521 ctcaccggcc cgcatcgcct tggactttga acaaaccggc atttccatgg atcaacaggt
    11581 actcgaatat gccgatcccc tgttgagcaa aatcagtgcc gcacaaaaca gcagccgtgc
    11641 gcgtctggtt ctgaatctga acaaaccggg ccaatacaat accgaagtac gcgggaacaa
    11701 agtttggata ttcattaacg aatcggacga taccgtgtcc gcccccgcac gccccgccgt
    11761 aaaagccgcg cctgccgcac cggcaaaaca acaggctgcc gcaccgtcta ccaagtccgc
    11821 agtatccgta tccaaaccct ttaccccggc aaaacaacag gctgccgcac cgtttaccga
    11881 gtccgtagta tccgtatccg caccgttcag cccggcaaaa caacaggcgg cggcatcagc
    11941 aaaacaacag acggcagcac cagcaaaaca acaggcggca acaccagcaa aacaaaccaa
    12001 tatcgatttc cgcaaagacg gcaaaaatgc cggcattatc gaattggctg cattgggctt
    12061 tgccgggcag cccgacatca gccaacagca cgaccacatc atcgttacgc tgaaaaacca
    12121 taccctgccg accacgctcc aacgcagttt ggatgtggca gactttaaaa caccggttca
    12181 aaaggttacg ctgaaacgcc tcaataacga cacccagctg attatcacaa cagctggcaa
    12241 ctgggaactc gtcaacaaat ccgccgcgcc cggatacttt accttccaag tcctgccgaa
    12301 aaaacaaaac ctcgagtcag gcggcgtgaa caatgcgccc aaaaccttca caggccggaa
    12361 aatctccctt gacttccaag atgtcgaaat ccgcaccatc ctgcagattt tggcaaaaga
    12421 atccgggatg aacattgttg ccagcgactc cgtcaacggc aaaatgaccc tctccctcaa
    12481 agacgtacct tgggatcagg ctttggattt ggttatgcag gcacgcaacc tcgatatgcg
    12541 ccagcaaggg aacatcgtca acatcgcgcc ccgcgacgag ctgcttgcca aagacaaagc
    12601 cttcttacag gcggaaaaag acattgccga tctgggcgcg ctgtattcac aaaacttcca
    12661 attgaaatac aaaaatgtgg aagaattccg cagcatcctg cgtttggaca atgccgacac
    12721 aaccggaaac cgcaatacgc ttgtcagcgg caggggcagc gtgctgatcg atcccgccac
    12781 caataccctg attgttaccg atacccgcag cgtcatcgaa aaattccgca aactgattga
    12841 cgaattggac gtacccgcgc aacaagtgat gattgaggcg cgtatcgtcg aagcggcaga
    12901 cggcttctcg cgcgatttgg gcgttaaatt cggcgcgaca ggcaagaaaa agctgaaaaa
    12961 tgatacaagc gcattcggct ggggggtaaa ctccggcttc ggcggcggcg ataaatgggg
    13021 ggccgaaacc aaaatcaacc tgccgattac cgctgccgca aacagcattt cgctggtgcg
    13081 cgcgatttcc tccggtgcct tgaatttgga attgtccgca tccgaatcgc tttcaaaaac
    13141 caaaacgctt gccaatccgc gcgtgctgac ccaaaaccgc aaagaggcca aaatcgaatc
    13201 cggttacgaa attcctttca ccgtaacctc aatcgcgaac ggcggcagca gcacgaacgc
    13261 ggaactcaaa aaagccgtct tggggctgac cgttacgccg aacatcacgc ccgacggcca
    13321 aatcattatg accgtcaaaa tcaacaagga ctcgcctgcg caatgtgcct ccggtaatca
    13381 gacgatcctg tgtatttcga ccaaaaacct gaatacgcag gctatggttg aaaacggcgg
    13441 cacattgatt gtcggcggta tttatgaaga agacaacggc aatacgctga ccaaagtccc
    13501 cctgttgggc gacatccccg ttatcggcaa cctctttaaa acacgcggga aaaaaaccga
    13561 ccgccgcgaa ctgctgattt tcattacccc gaggattatg ggtacggccg gcaacagcct
    13621 gcgctattga tgcgtcaaaa taagggcata tgttttacgg catatgccct ttctttatgc
    13681 tttttgccgc gaccgaaatg ccgtcattcc cgcgcaggca gaaatccaac ttgtccggtt
    13741 ccggttgttt ttcgtctcgt aacttttgag ccgtcattcc cgcgcaggca gaaatccagt
    13801 ccgttcagtt tcggtcattt ccgataaatt cctgttgctt ttcatttctg gattccc
//
LOCUS       DAWHXZ010000028        13838 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000028 DAWHXZ010000000
VERSION     DAWHXZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.028"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..286
                     /locus_tag="WIE35_000546"
     CDS             <1..286
                     /locus_tag="WIE35_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ4153509.1"
                     /translation="DKGYDSAENRQHLEEHQLQDGIMRKACRNRPLTEAQTKRNRYVV
                     EQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            422..541
                     /locus_tag="WIE35_000547"
     CDS             422..541
                     /locus_tag="WIE35_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ4153510.1"
                     /translation="MQRSHKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            552..1522
                     /locus_tag="WIE35_000548"
                     /pseudo
     CDS             552..1522
                     /locus_tag="WIE35_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
     gene            complement(1573..2625)
                     /gene="sohB"
                     /locus_tag="WIE35_000549"
     CDS             complement(1573..2625)
                     /gene="sohB"
                     /locus_tag="WIE35_000549"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ4153511.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(2729..2818)
                     /locus_tag="WIE35_000550"
     tRNA            complement(2729..2818)
                     /locus_tag="WIE35_000550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2782..2784),aa:Leu,seq:taa)
     gene            complement(2921..2994)
                     /locus_tag="WIE35_000551"
     tRNA            complement(2921..2994)
                     /locus_tag="WIE35_000551"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2960..2962),aa:Cys,seq:gca)
     gene            complement(3020..3095)
                     /locus_tag="WIE35_000552"
     tRNA            complement(3020..3095)
                     /locus_tag="WIE35_000552"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3060..3062),aa:Gly,seq:gcc)
     gene            complement(3117..3192)
                     /locus_tag="WIE35_000553"
     tRNA            complement(3117..3192)
                     /locus_tag="WIE35_000553"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3157..3159),aa:Gly,seq:gcc)
     gene            complement(3216..3291)
                     /locus_tag="WIE35_000554"
     tRNA            complement(3216..3291)
                     /locus_tag="WIE35_000554"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3256..3258),aa:Gly,seq:gcc)
     gene            complement(3317..3392)
                     /locus_tag="WIE35_000555"
     tRNA            complement(3317..3392)
                     /locus_tag="WIE35_000555"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3357..3359),aa:Gly,seq:gcc)
     gene            complement(3472..4035)
                     /gene="pgsA"
                     /locus_tag="WIE35_000556"
     CDS             complement(3472..4035)
                     /gene="pgsA"
                     /locus_tag="WIE35_000556"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ4153512.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            complement(4141..4564)
                     /locus_tag="WIE35_000557"
                     /pseudo
     CDS             complement(4141..4564)
                     /locus_tag="WIE35_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4698..4763)
                     /locus_tag="WIE35_000558"
                     /pseudo
     CDS             complement(4698..4763)
                     /locus_tag="WIE35_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4896..5231)
                     /locus_tag="WIE35_000559"
     CDS             complement(4896..5231)
                     /locus_tag="WIE35_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ4153513.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            5360..6265
                     /gene="mtrA"
                     /locus_tag="WIE35_000560"
     CDS             5360..6265
                     /gene="mtrA"
                     /locus_tag="WIE35_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ4153514.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     VVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(6327..6815)
                     /locus_tag="WIE35_000561"
     CDS             complement(6327..6815)
                     /locus_tag="WIE35_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ4153515.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            6962..7864
                     /locus_tag="WIE35_000562"
     CDS             6962..7864
                     /locus_tag="WIE35_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ4153516.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLAEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(7905..9035)
                     /locus_tag="WIE35_000563"
     CDS             complement(7905..9035)
                     /locus_tag="WIE35_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4153517.1"
                     /translation="MTKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            9240..11864
                     /gene="alaS"
                     /locus_tag="WIE35_000564"
     CDS             9240..11864
                     /gene="alaS"
                     /locus_tag="WIE35_000564"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ4153518.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYVSDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSMD
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGDAYPELKEKQVQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGEHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELAKAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            11926..12657
                     /locus_tag="WIE35_000565"
     CDS             11926..12657
                     /locus_tag="WIE35_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153519.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFFGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            12964..>13838
                     /locus_tag="WIE35_000566"
     CDS             12964..>13838
                     /locus_tag="WIE35_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ4153520.1"
                     /translation="MKTLLQLQTAAQNFAAYYKDQTDERREKDTFWNEFFAIFGIDRK
                     NVAHFEYPVKDPADNTQFVDIFWEGIFLAEHKSANKNLTKAKEQAERYLQEIGRTKPS
                     ALPEYYAVSDFAHFHLYRRVPEEGVENQWQFPLEALPEYITRGVFDFMFGIEAKVRQI
                     QEEADIQAAAAIGRLHDALKEEGIYEEHELRLFITRLLFLFFADDSAVFQRNYLFQDF
                     LESCKETDTLGDKLNQLFEFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTA
                     KQHRALIDCGNFDWR"
BASE COUNT         3504 a         3606 c         3333 g         3395 t
ORIGIN      
        1 cgacaaaggc tacgacagtg cggaaaaccg gcaacatctg gaagaacatc agttgcagga
       61 cggcattatg cgcaaagcct gccgcaaccg tccgctgacg gaggcacaaa ccaaacgcaa
      121 ccgttatgtg gtcgaacaaa gcttcggtac gctgcaccgt aaattccgct acgcccgggc
      181 agcctatttc ggactgatta aagtgagtgc gcaaagccat ctgaaagcga tgtgtttgaa
      241 cctgttgaaa gccgccaaca ggctaagtgc gcccgctgcc gcctaaaagg cagcccggat
      301 gcctaattat cgggcgtccg gtgaggatta agggggtatt tgggtagaat taggagtaat
      361 taggagcgaa aatagacgaa aacctgtgtt tgggtttcgg ctgtcgggag ggaaaggaat
      421 tttgcaaagg tctcacaaag tatttgccac attcggcgac aagcgcacaa cccggctgtt
      481 tgccgatttg gatgcactgg ctgaagtgat ggaaaaaaca atctcggaaa ataaaaaata
      541 ggggggcaaa tatgtggaaa atgttgaaac acatagccca aacccaccgc aagcgattga
      601 ttggcacatt ttccctggtc ggactggaaa accttttgat gctggtgtat ccggtgtttg
      661 gcggccgggc gatcaatgcc gtgattgcgg gggaggtgtg gcaggcgttg ctgtacgctt
      721 tggttgtgct tttaatgtgg ctggtcggtg cggcgcggcg gattgccgat acacgcacgt
      781 ttacgcggat ttataccgaa atcgccgtgc cggttgtgtt ggaacagcgg cagcggtaag
      841 tcccgcattc ggcaatatag tggattaaat ttaaaccagt acggcgttgt ctcgccttgc
      901 cgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
      961 aactgcgcgg gttgccctgt cgcgtgagtt tgtcagcttt tttgaagaac acctgccgat
     1021 tgccgcgaca tccgtcgtat ccatattcgg cgcgtgcatc atgctgctgg tgctggaatt
     1081 ttgggtcggc gtgtcggcgg tgggcatact tgcgttgttt ttatggcttt tgccacgttt
     1141 tgcggccatc agcgaaaacc tgtatttccg cctgaagaac agcttggaac gcgacaacca
     1201 ctttatccga aaaggcgacc ggcggcagct gtaccgccat tacggactgc ttgcgcgcct
     1261 gcgtgtgctg atttccaacc gcgaagcctt cggctatctc tgcgtcggca cggcgatggg
     1321 tattttgttc ggctttgctt ttgtgatgat gacgctcaaa ggctacagca gcgcggggca
     1381 tgtctattcg gtcggcactt atctgtggat gtttgccatg agtttggacg acgtgccgcg
     1441 attggtcgaa caatattcca atttgaaaga catcggacaa cggatagagt ggtcggaacg
     1501 gaacatcaaa gccggaactt gaaaaatgcc gtctgaacac gcttcagacg gcatttccat
     1561 ccgttcggca aactacatca catccgcccg ccggttgaca agtttggcaa acaacttttc
     1621 aacagaagct tccgcctgca aaccaatgcg ctggatcagg ctttgcttct cctgatattt
     1681 cacttcgata acctgtttgt tttcaaacgc tttcaacaac aaatcatcac tggtcgaaat
     1741 ctcgtcaatc aagttcaacg ccaacgcctg ccgaccgaac caatgctcgc ccgttgccac
     1801 ttcctcaata tccaattgag ggcggttctc gctgacaaac tgcttgaaca actgatgcgt
     1861 ttcctccagt tcctgtcgga atttctgttt gcccttttcc gtattttcac ccataaaagt
     1921 aaccgtgcgc ttaaattcgc ccgccgtcat cacatccaca tcaatatcat gttttttcaa
     1981 caggcggtgg atattcggta cttccgccac cacacccacc gaaccgacaa tcgcaaacgg
     2041 agcggaaaca attttatccg ccacacacgc catcatataa ccgccgctcg ccgccacctt
     2101 atcgacggcg acggtcagcg gaatattgcg ttcgcgcaaa cgcctaagct gtgaagccgc
     2161 caaaccgtaa ccgtgaacca cgccgcccgg actttccaat ctaagcagaa cctcatcttc
     2221 aggcttggca atcaaaagca ccgccgtaat ctcatgacgc aaggattcta cggcgtgtgc
     2281 atacaaatcg ccgtcaaaat ccaacacaaa aaggcgggat ttttgcgttt cggcagattt
     2341 ctccccaccc tccttcaaac gctttttctc tgctttggct tccgcctttt cctttttctt
     2401 ttcctctttt tcctgatgtt ttgcctcttc cccgcttaaa aagaatgctt caaacgattg
     2461 ccgctgtttt ttataatttt ccgaaaaatc cgtcagtacg acactgccgc tttccgactg
     2521 tttcttactc tgtacgatag ccaacacaat cagcgcaatt gcgccgaaca cggtaagcag
     2581 ttcgagcagg aaaataccgt aattcagtaa aatttctttc cacattgatt ggatttcctc
     2641 ttgttcaggc atgaacatgt caatattgtc catcaccgtc cgacagataa aaaaataacc
     2701 gcttggagcg gcattgtcat tttcagcttg gtgcccggag ccggaatcga accggcacgg
     2761 gatgtttagt cccgacggat tttaagtccg ttgtgtctac ctatttcacc acccgggcat
     2821 ttgtgaaagg tggaggcggg ggcgcggatt ttaaccggcc tatatgaaga ttgcactcct
     2881 catagcataa acactctgcc accccgccat agtacgataa tggaggcgag agtcggaatc
     2941 gaaccggcgt agacggattt gcaatccgct gcataaccac tttgctatct cgccctaaaa
     3001 ctggcttatc taaaaaactt ggagcgggaa acgagtctcg aactcgcgac ctcaaccttg
     3061 gcaaggttgc gctctaccaa ctgagctatt cccgcgcgtt caaacatatc ggtttttgga
     3121 gcggaaaacg agtctcgaac tcgcgacctc aaccttggca aggttgcgct ctaccaactg
     3181 agctattccc gcgttgatat gtttgaaata aaacttggag cgggaaacga gtctcgaact
     3241 cgcgacctca accttggcaa ggttgcgctc taccaactga gctattcccg caatgattgt
     3301 gggaaaatga aatttttgga gcgggaaacg agtctcgaac tcgcgacctc aaccttggca
     3361 aggttgcgct ctaccaactg agctattccc gcccgatttc attctccaac atcgaagaga
     3421 cgcaattatt atggattctg tttttgccgt caagctattt ttatgttttt ttcaggcgat
     3481 ttctttccac gccattttca gataatacag catcgaccag actgtcagca aagatgcgat
     3541 aaacatcaat acattgccga taacaacaag attaaatccg taaaaatcgg gaatattcag
     3601 cagcagcagg aagattgcca gcatttgcgc ggcggttttg aacttaccga cggtggcgac
     3661 ggcaacgctg ttccttttgc ccatttgcgc catccattcg cgcaatgcgg aaatggtaat
     3721 ttccctgccg ataatgatca tggcaaacaa aacataggtc cggtcgagtt tgaccagtaa
     3781 aagtagcgat acggcaacca tcagcttgtc ggcgacggga tcgaggaagg cgccgaaatc
     3841 ggaagtctgt ttccacagtc ttgccaaaaa tccgtcaaac cagtcggtta aggcggcaac
     3901 ggcaaaaatg acggcggcgg tgagattaat cgtttcctcc gcgaaccacg gaaaaggcag
     3961 gtaaaaaagg gctgtcatga caggaatgag caagaccctc aaccatgtga ggaagatggg
     4021 gagattccaa ggcatcgatt ttctctgtgc agactgtaaa gttgtgatta taacggttat
     4081 cctcataacc caaaacgtaa aattgctgca tgggcattcc cccgccccgc caatctgttt
     4141 tcacattctt ttcaaacgca ggaaaatggc gggcaataaa agcaaaatac ccagtttcag
     4201 gctgaaaacg gcaggttgtg ccaacacttc gacaaggcgg tcttccgtgc gggcaaaatc
     4261 tttattgctt atagacactg ccactgttgc ggtattccaa cagaacgccg tttaaaaaac
     4321 ctttgccgac ggtttcgctt aaaacggctc taacctgctc cgccctgatg gttctgccga
     4381 tattgccgcc tgtgcacaaa ctgtcgaacc catagcagga aagccggtaa tgctgcccgt
     4441 ctgcatccag tttgattgcc cgtccgctgc ggttgggggc ggtaacggtc aattccgcat
     4501 attctaatgt tttttcttgt tcgtgaaatg ccgtcaggta aggtgcaata aaaacggcgg
     4561 acaacagcag acagcttatg gcggcaaacc atacccagcg ataatatagt ggattaacaa
     4621 aaaccagtac agcgttgcct cgccttagct caaagagaac gattctctaa ggtgctgaag
     4681 caccaagtga atcggttccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
     4741 tttaaattta atccactata tttcacgctt accccttgtt tctcaaatgc cgtctgaaaa
     4801 aaacggctgt atatgttgtc tgcaaaatat cgggaagcat aacagacaaa atgccgtctg
     4861 aaatattttc agacggcatt tcaatccgat tcggattatt tttgcgtttc aaccgcttcc
     4921 aatgcacgca gggcgtaagt gtaagcggca ccggcattca gggcgatggc ggttgccaat
     4981 gcgccggcga tttcgctgtc ggtcgcaccg gctttggcgg cggcggcggc gtgaacactg
     5041 atgcagctct cacaacgtgt ggtaatggca acggcgatgg caatcagttc gcgcgttttg
     5101 gcatcaagtg cttcggcggc ggcggcctgt tccaatgcgc cgtaggcctg cagcatttta
     5161 ggatgcgcct tacccagctc gccgaacgat tttttaacca atgcggtatg ttctttccaa
     5221 tctttaaaca ttttcttttc ctttctcttg cgtttaaccc tgatacgcgc ttgcgtatct
     5281 gttttcgatg tgcgtattat tgcaattatt cagttgtgtt tctcgtttaa tcatctcatt
     5341 ttatggttca aaaagattta tggacattct agacaaactg gtcgatttcg cccaattgac
     5401 gggcagtgtg gatgtgcagt gccttttggg cggacaatgg tcggtacggc atgaaacctt
     5461 gcaacgcgaa ggattggtac acattgttac atcgggcagc ggctatctct gcatcgacgg
     5521 cgaaacttcc ccgcgtccgg tcagtacagg ggatattgta tttttcccgc gcggcttggg
     5581 tcatgtgttg agccacgacg gaaaatgcgg agaaagttta caaccggata tgcggcagca
     5641 cggtgcgttt acggtcaaac agtgcggcaa cggacaggat atgagcctgt tttgcgcccg
     5701 tttccgctac gacacccacg ccgatttgat gaacgggctg cctgaaaccg tttttctgaa
     5761 cattgcccat ccgagtttac agtatgtggt ttcaatgctg caactggaaa gcaaaaaacc
     5821 tttgacgggg acggtttccg tggtcaacgc attgtcgtcc gtcctgctgg tgcttatcct
     5881 gcgcgcctat ctcgaacagg ataaggatgt cgaactctcg ggcgtattga aaggttggca
     5941 ggacaaacgt ttgggacatt taatccaaaa ggtgatagac aaaccggaag acgaatggaa
     6001 tgtcgacaaa atggtggcgg ctgccaatat gtcgcgcgcg caactgatgc gccgtttcaa
     6061 aagccgggtc ggactcagcc cgcacgcctt tgtgaaccat atccgcctgc aaaaaggcgc
     6121 gttgctgctg aaaaaaaacc cggattcggt tttgtcggtc gcactgtcgg taggctttca
     6181 gtcggaaacg cacttcggca aggcgttcaa acggcaatat cacgtttcgc cgggtcaata
     6241 ccggaaagaa ggcgggcaaa aataaatcgg ggcttcaaac gcaaatgccg tctgaaaagg
     6301 ctttcataca gcatttgcgt accgcgtcat ttcaagggct gcatcttcat cacttccatc
     6361 aaaaagttgg taaatgcggg gttgttgggt ttgacatcca tatttttcca acgctgctgc
     6421 cagccgcgca aggcattctg gatatacagc ttggactgtt ccgtattgat tgcgccccgc
     6481 tggctgtcta tcgccgaacg caggtagatt tcatacatac tgtcatcgac ggcattgcgt
     6541 ccgaccaggc gttttctgaa gttgttcaga tattgcgccg cctgaacctt ggtcatttta
     6601 ccgataccca cctgatagcc caagcgcgtc gcttcatcgc tgattttggc aacatccgtc
     6661 caatgcgaag aggcaaggcg gaaacctttt gcaggtgctt ccgttttgac ggtattgata
     6721 ggattcacgg ggatttccgt caatgtgggc acataaatag actggcagcc ggaaagaact
     6781 gccacaatgg aaagagggat aaggtatttt ttcatgcccc cattataatc aagtttgcct
     6841 tgagaaaaca aattgttcgg caagaaaaat aaaatttcgg catcagaagc aggcaaaaac
     6901 acattccaca agccttgccg caaggtttac aatccgaccg tccttatcgc aacgaccgtt
     6961 tatggatacc gcaaaaaaag acattttagg atcgggctgg atgctggtgg cggcggcctg
     7021 ctttaccatt atgaacgtat tgattaaaga ggcatcggca aaatttgccc tcggcagcgg
     7081 cgaattggtc ttttggcgca tgctgttttc aaccgttgcg ctcggggctg ccgccgtatt
     7141 gcgtcgggac accttccgca cgccccattg gaaaaaccac ttaaaccgca gtatggtcgg
     7201 gacgggggcg atgctgctgc tgttttacgc ggtaacgcat ctgcctttgg ccaccggcgt
     7261 taccctgagt tacacctcgt cgattttttt ggcggtattt tccttcctga ttttgaaaga
     7321 acggatttcc gtttacacgc aggcggtgct gctccttggt tttgccggcg tggtattgct
     7381 gcttaatccc tcgttccgca gcggtcagga aacggcggca ctcgccgggc tggcgggcgg
     7441 cgcgatgtcc ggctgggcgt atttgaaagt gcgcgaactg tctttggcgg gcgaacccgg
     7501 ctggcgcgtc gtgttttacc tttccgtgac aggtgtggcg atgtcgtcgg tttgggcgac
     7561 gctgaccggc tggcacaccc tgtcctttcc atcggcagtt tatctgtcgt gcatcggcgt
     7621 gtccgcgctg attgcccaac tgtcgatgac gcgcgcctac aaagtcggcg acaaattcac
     7681 ggttgcctcg ctttcctata tgaccgtcgt tttttccgct ctgtctgccg cattttttct
     7741 ggccgaagag cttttctggc aggaaatact cggtatgtgc atcatcatcc tcagcggtat
     7801 tttgagcagc atccgcccca ctgccttcaa acagcggctg caatccctgt tccgccaaag
     7861 ataaaaaatg ccgtccgaac atccttcaga cggcatatcg ggctttattt ccccgccttc
     7921 acatcctgcc actggcgcac cataaacttc aatgccgccg gctggatagg caccatgata
     7981 aagctgtttt tcaaatcctc ctcggttggg aaaatcgtat tgtcgttttt aaattcgtct
     8041 tccatcagct cacgcgcagg cttgctcgaa ggcgcgtaag taacgaaatt gccgtttttc
     8101 gccgacactt ccgggtcgag gaagtcgttg atgtatttgt gcgcgttggc gacgtttttc
     8161 gcatctttcg gaatcacgaa agaatccacc caaatcccca cgccctcttt gggcatcatc
     8221 acgcggattt tttccttgcc gcccgcttct tcggcacggc gtttggcgat gttcaaatcg
     8281 ccgccgaaac cgattgttac gcaggtatcg ccgcgcgcca aatcatcgat aaagccggac
     8341 gaagtaaagc gtttgatatt ggggcggttt ttcttgagta gggcggttgc ctccctgatg
     8401 tcttccgtat tgctgctgtt cgggttttta cccaaatagt tcaacaccat aggatagatt
     8461 tccgccgcgc tgtccaaata gctgatgccg cattgcttga gtttggacgt gtattcgggg
     8521 tcgaacacca aatcccactg gttgtccggc agcttgtccg tacccaaagc ctttttcacg
     8581 cgttcggtat tgatggcgaa ggtatttgtc ccccaataaa acggcacggc gtattcgtgg
     8641 ccgggatcga ccccgtccat cagcctcatc atttcggggt tgaggtgttt ataattggga
     8701 atcagcgact tatcgatttt ctgatacgca cctgccttaa tctgcctgcc cacaaacgca
     8761 ttggacggcg cgacaatgtc gtaaccagat tttccggtca gcaccttgct ttccagcgtt
     8821 tcatcgctgt cgtacacatc ataagtaacc ttgatgccgt ttttcttttc aaaatcggca
     8881 acggtttccg gatcgacgta ttccgaccag ttgtaaattt tcaatacgtt tcggttttcc
     8941 gccggcgccg gtttttcggc aggcggtttg tccgaaccgc cgcacgctgc aagcagcaaa
     9001 gcagtcagga cggccagggg cagatgtttg gtcattatca ttccttgcat atcgggttgg
     9061 agaaagcggc cattatagcc gatattggca acagggcttc agacggcatt caaaatcccg
     9121 ccacactctt ccgaaaaccg ccgcttccat agctagaaac agggatttgc ggtaagatac
     9181 cgccgttcgt tttccctgct tttaccatga caagacattt gagagacatt gaaaaaatta
     9241 tgaaaacctc cgaactgcgc caaaaattcc taaaattttt tgaaaccaaa ggccacaccg
     9301 tcgtccgctc ttccagcctc gtgccgcacg acgacccgac cctgctgttt accaacgcgg
     9361 gcatgaacca gtttaaagac gtattcttag gtttcgacaa acgcccgtac agccgcgcca
     9421 ccaccgcgca aaaatgcgta cgcgcaggcg gcaagcacaa cgacttggaa aacgtcggct
     9481 acaccgcccg ccaccacacc ttctttgaaa tgatgggcaa cttctccttc ggcgactact
     9541 tcaaacgcga cgccatccac ttcgcttggg aatttctgac ttcccccgaa tggctcaaca
     9601 tccctaaaga caaactgttg gcgaccgttt acgcggaaga cgacgaagcc tacaacatct
     9661 ggttgaacga aatcggtatg ccgtccgagc gcatcgtccg catcggcgac aacaaaggcg
     9721 cgaaatacgt gtccgacaac ttctggcaaa tgggcgacac cggcccttgc ggcccctgct
     9781 ccgaaatttt ctacgaccac ggcgaagaaa tctggggcgg cattcccggc agccccgaag
     9841 aagacggcga ccgctggatc gaaatttgga actgcgtatt tatgcagttc aaccgcgacg
     9901 aacaaggcaa tatgaatccg ctgcccaagc cgtccgtcga taccggtatg ggcttggaac
     9961 gtatggccgc cgtcatgcag catgttcaca gcaactacga aatcgacttg ttccaagacc
    10021 tgctcaaagc cgttgcccgc gaaaccggcg cagctttcag catggatgaa cccagcctga
    10081 aagtcatcgc cgaccacatc cgctcctgct cgttcttgat tgcagacggc gtgttgcctt
    10141 ccaacgaagg ccgcggctac gtattgcgcc gcatcatccg ccgcgccgtg cgccacggtt
    10201 acaaactggg tcaaagcaaa ccgttcttcc acaaactggt ggccgatttg gtcaaagaga
    10261 tgggcgatgc ctaccctgaa ttgaaagaaa aacaagttca aatcgaagaa gcgttgaaaa
    10321 acgaagaaag ccgttttgcc caaacgctgg aaaccggtat ggctttgttg gaaaacgcgt
    10381 tggctaaagg cggcaaaaca ctcgacggcg aaatcatctt caaactctac gatacttacg
    10441 gtttcccata cgatttgacc gccgacatct gccgcgaacg caatatcgaa ctggacgaag
    10501 caggcttcga gcgcgaaatg gaagcccaac gcgcacgcgc acgcgccgcc caaagcttca
    10561 aagccaacgc ccaactgcct tatgacggtc aagacaccga gtttaaaggt tatagcgaac
    10621 gccaaaccga atccaaagtc ctcgccctct acaaagacgg cgagcaagtc gacgaattga
    10681 acgaaggcga cagcggcgca gtcgtcatcg actttacccc gttctatgca gaatccggcg
    10741 gccaagtcgg cgacgtcggc tatatcttct caggcgaaaa ccgctttgaa gtacgcgata
    10801 cccaaaaaat caaagcagcc gtattcggcc aattcggtgt acaaacttca ggccgtctga
    10861 aagtcggcga cagcgttacc gccaaagtgg acgacgaaat ccgcaatgcc aatatgcgca
    10921 accacagcgc gacccacctg atgcacaaag ccctgcgcga cgtattgggc gaacacgtcg
    10981 aacaaaaagg ctctttggtt accgccgaat ccacccgttt cgacatttcc catccccaag
    11041 cggtaactgc cgaagaaatt gccgaagtcg aacgccgcgt caacgaagcc attttggcga
    11101 acgttgccgt caatgcagcc attatgagca tggaagacgc gcaaaaaacc ggcgcgatga
    11161 tgcttttcgg cgaaaaatac ggcgacgaag tgcgcgtact gcaaatgggc ggtttctcaa
    11221 ccgaattgtg cggcggcaca cacgtttcac gcaccggcga catcggcctc ttcaaaatca
    11281 tcagcgaagg cggtattgcc gcaggcgtgc gccgtatcga agccatcacc ggcctgaacg
    11341 cactcaaatg ggcgcaagag caagagcgtt tggtgaaaga cattattgct gaaaccaaag
    11401 cccaaaccga aaaagacgta ctggcgaaaa tccaagcagg cgcggcacac gccaaagcct
    11461 tggaaaaaga gttggcaaaa gccaaagccg aactcgccgt ccacgcaggc gcaaaactct
    11521 tggacgacgc aaaagacttg ggcgcagcca aactcgttgc tgcccaaatc gaagccgacg
    11581 cagccgccct gcgcgaaatc gttaccgatt taaccggtaa atccgacaac gccgtgattc
    11641 ttttagcggc agtaaacgac ggcaaagtct ccctgtgcgc cggcgtatcc aaaccgttga
    11701 ccggcaaagt gaaagcaggc gatctggtta aatttgcagc cgaacaagtc ggcggcaaag
    11761 gaggcggccg tcccgacttg gcgcaggccg gcggcacgga tgccggcaaa ttgcccgaaa
    11821 tgctggtaag cgtggaaagc tggctttgtc agaaattaag ttaaatttaa atcttaataa
    11881 gccatttgag tcttgttcaa atggcttata ttttggattg ttagaatgaa tactaaaaca
    11941 gagttacaaa aacttttaga agaggatata tcaactttaa cagaaacact aatttgtgca
    12001 gatgctcttc ctccaagata tgtacgtagc attgcaactc ctattgtccg tcgatggtta
    12061 attgacaaac aattaaatat tctcgcaaaa gaaataggtt taactataga attgcccatc
    12121 cttgatacaa gtttggtatt tgaaaaattg tcaaccttag agaataaagt taatttttat
    12181 atggctggtg gagtttattt aggcggagaa tttattagct ctatatatca ttcatcacaa
    12241 gaattttttg gtgaaccaat aatctatgct gaaccaaata taattttatg tcctgctgaa
    12301 aaatttttaa ctttaaaacg agttttccat aatggaaata tttttaatat gaatcagata
    12361 attacttttt tatccaataa acaaggagga gtacattttg ataaaaatta cgataaatat
    12421 aaaacttggc aagttgctat agaaaaagca gcaaattttt taaaactcgg taatccatat
    12481 aatgaagata aattatctct tagtgaagaa catgatacta ttttagtagt tttaccatta
    12541 gaaaaaggat atgaatggaa ttgtcttgag atagaagtac tatccgctgc tcaatctcta
    12601 gcaaatatct attgcaacaa agttcgcctc atagatggtc atgtttggaa ggaataaaag
    12661 aaaaagcata gttggtaatt tttagttacg tagggtgggc ttcagcccac cgttttttca
    12721 tttttgattg ttgattggtg ggctaaagcc caccctacgg ttactaaaat cccgtcatta
    12781 cctcgcaggc gggaatccgg tttgttcggt ttcggttttt tagggacagt gccgcaacat
    12841 taaatttcca gattcccact ttcgcacaaa taacagcgca aatagggcaa agataaggat
    12901 atgggttttt ttctttgaat ttactataca attcgcccgt ccccgttata gaaacccaag
    12961 ccgatgaaaa ccctgctcca actccaaacc gccgcacaaa acttcgccgc ctactacaaa
    13021 gaccaaaccg acgaacgccg cgagaaagac accttctgga acgaattttt cgccattttc
    13081 ggcatcgacc gcaaaaacgt cgcccacttc gaataccccg tcaaagaccc tgccgacaac
    13141 acccaattcg tcgatatatt ttgggagggc atcttccttg ccgaacacaa atccgccaac
    13201 aaaaacctga ccaaggccaa agagcaggcg gagcgttatt tgcaggaaat cgggcgcacc
    13261 aagccctctg cgctgcccga atattacgcc gtcagcgatt ttgcccattt ccacctttat
    13321 cgccgcgtac ctgaagaagg cgtggaaaac caatggcagt tccctttgga agcattgccc
    13381 gaatacatca cgcgcggcgt tttcgacttc atgttcggca tcgaagccaa agtccgccaa
    13441 attcaagaag aagccgatat tcaagcggcg gcggccatcg gcaggctgca cgacgcgctc
    13501 aaagaagaag gcatttacga agaacacgag ctgcgcctct tcatcacgcg cctgcttttc
    13561 ctcttttttg ccgacgacag cgccgttttc cagcgcaact atctctttca agacttttta
    13621 gaaagctgca aagaaaccga cacgctcggc gacaagctca accaactctt tgaattcctc
    13681 aacacacccg accaaaagcg cagcaagacc caaagcgaaa aatttaaagg tttcgaatac
    13741 gtcaacggcg gcctgtttaa agaacgcctg cgcaccttcg acttcaccgc caagcagcac
    13801 cgcgccttaa tcgactgcgg caatttcgac tggcgcaa
//
LOCUS       DAWHXZ010000029        13797 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000029 DAWHXZ010000000
VERSION     DAWHXZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.029"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..278)
                     /locus_tag="WIE35_000567"
     CDS             complement(<1..278)
                     /locus_tag="WIE35_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ4153521.1"
                     /translation="MITVEHLEHYLGEIENGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPI"
     gene            complement(275..1045)
                     /locus_tag="WIE35_000568"
     CDS             complement(275..1045)
                     /locus_tag="WIE35_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ4153522.1"
                     /translation="MVKTADGYKAIARIRVGDRVFAKDEASGKTGYKPVTTRYGNPYQ
                     ETVYIEISDGIGNNQTLISNSIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGDRAETEGVWVHNDCPYGSSSAKGKSGSERNTAKRGT
                     FRQETLKNAWDLAADGSQPNSKKCPTCGKDVYGNPHNKEKRNTKDGWDGSHNPSWSNR
                     QHSSDRKEQLNDYNKGVSLECRHCNRSGGNKDERFKKK"
     gene            complement(1260..1619)
                     /locus_tag="WIE35_000569"
     CDS             complement(1260..1619)
                     /locus_tag="WIE35_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153523.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKSGWVFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDS
                     YKVHWKINNGNLIQTTN"
     gene            complement(1840..2295)
                     /locus_tag="WIE35_000570"
     CDS             complement(1840..2295)
                     /locus_tag="WIE35_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153524.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILGTFMYEPPDEF
                     RTEYYESPDSNLLIAVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
     gene            complement(2276..2905)
                     /locus_tag="WIE35_000571"
     CDS             complement(2276..2905)
                     /locus_tag="WIE35_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ4153525.1"
                     /translation="MVKTADGYKAIARIRTGDRVFSKDEASGKTGYKPVTARYGNPYQ
                     ETVYIKVSDGIGNSQTLISNRIHPFYSGGKWIKAEDLKAGSRLLSESGKTQTVRNIVV
                     KPTPLKAYNLTVADWHTYFVKGSQAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQIS
                     AESRIAELKNKIIPGMPKNERLKIEKTIKNITKNANRKAKGEEHGRRGR"
     gene            complement(3039..3356)
                     /locus_tag="WIE35_000572"
     CDS             complement(3039..3356)
                     /locus_tag="WIE35_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153526.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(3359..4627)
                     /gene="mafB"
                     /locus_tag="WIE35_000573"
     CDS             complement(3359..4627)
                     /gene="mafB"
                     /locus_tag="WIE35_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ4153527.1"
                     /translation="MKPLRRLTNLLAACAVAVAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNPFISAGEAVDQWMQENPNAAETVETLVNVLPFAKVKNLT
                     KAAKPGKAAVSGDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGK
                     HISGHRNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKS
                     GLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
     gene            complement(4631..5572)
                     /gene="mafA"
                     /locus_tag="WIE35_000574"
     CDS             complement(4631..5572)
                     /gene="mafA"
                     /locus_tag="WIE35_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ4153528.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTRNSGRKGERSAGLSVNGMGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWTGPYKVSKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            complement(5691..6791)
                     /locus_tag="WIE35_000575"
     CDS             complement(5691..6791)
                     /locus_tag="WIE35_000575"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ4153529.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGTDELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRAVLNIGGIANISVLPSDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFARPHPKSTGRELFALNWLETYLGGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAANARQMYICGGGIRNPVLMADLAECFGTRVSLHSTADLNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            7116..8690
                     /locus_tag="WIE35_000576"
     CDS             7116..8690
                     /locus_tag="WIE35_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ4153530.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            complement(9043..10464)
                     /gene="hemN"
                     /locus_tag="WIE35_000577"
     CDS             complement(9043..10464)
                     /gene="hemN"
                     /locus_tag="WIE35_000577"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ4153531.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            10669..11403
                     /gene="fnr"
                     /locus_tag="WIE35_000578"
     CDS             10669..11403
                     /gene="fnr"
                     /locus_tag="WIE35_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ4153532.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            complement(11516..12466)
                     /locus_tag="WIE35_000579"
     CDS             complement(11516..12466)
                     /locus_tag="WIE35_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ4153533.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDALKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            12830..13558
                     /gene="rmpM"
                     /locus_tag="WIE35_000580"
     CDS             12830..13558
                     /gene="rmpM"
                     /locus_tag="WIE35_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ4153534.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
BASE COUNT         3092 a         3194 c         3657 g         3854 t
ORIGIN      
        1 aaaataggtt gtctattgtc aatcaaatat tcagccactc ctgatagaaa agctgcaata
       61 ttttctttat tccattcttc agaacaggta aaaattaatt caaacctact tttatagttc
      121 aagtcatatc gattcaagcc taaagtagaa aaagtagtta cccctttata tggttgtgat
      181 gggaagacgg aaacgctgag atgataacgt cgatccaagc attttatacc gttttcgatt
      241 tcacccaaat aatgctctaa atgttcgact gtaatcattt tttcttaaac ctttcatcct
      301 tgttgccacc agatcgattg caatgtctgc attctaaact tactccttta ttataatcat
      361 ttagttgctc cttacgatca gaactatgtt gacggttact ccatgatgga ttatgacttc
      421 catcccaacc atcttttgta ttacgtttct ccttgttatg gggattacca tatacatctt
      481 taccacatgt aggacatttt tttgaattag gctggctacc atctgctgcc aaatcccatg
      541 cattttttag cgtttcttgt ctgaaggttc cacgtttagc agtatttctc tcactgccac
      601 ttttaccttt tgcgctagaa ctaccatacg gacaatcatt atgaacccaa accccttccg
      661 tttccgccct atcacccttg acgaagtaag tatgccaatc ggcaacggtc agattgtagg
      721 ctttgagcgg ctgctgtttg agggtaatgt tttgaaccgt ctgttttgca ccgctttcgg
      781 aaagcagggt gtcgcctttt ttcagacgac ctgcctgtat ccattttcct tgactgtaaa
      841 acggatggat gctgttggaa atcagggttt ggttgttgcc gatgccgtct gaaatttcaa
      901 tgtaaacggt ttcctggtac ggattgccgt atcgggtggt aacgggtttg tatcctgttt
      961 ttccgcttgc ctcgtccttg gcaaagacgc ggtcgccgac tcggatacgg gcaatggctt
     1021 tgtagccgtc tgccgttttg accaaggtgc tgccgtggaa ggagcaggtg taggattttt
     1081 aaagaactta gttctcaata tcctgtttca ttcatcaaaa tgccgtctga aagctgaata
     1141 ccgcttcaga cggcattttg gtggttgggt ttttaagcca acctacgctt actgaaaacc
     1201 aaattgagtt tcagacagtt tttaggtttg ggtgtccaat ctaacttata tttgtccatt
     1261 taattagtcg tttgtatcaa atttccatta tttattttcc aatgtacttt ataactatct
     1321 tcataataat ctaattcaaa aaaacctgat atttcaatat ccaattccat tattgtttta
     1381 atgcattttt caaaataaat aatgaaataa gattttacgc atgcaccaaa aaaaatatag
     1441 ctgctccaat taaaactatt tgtcgggaaa acccacccgc ttttatatat ttttgcagat
     1501 tctttctctt cgatattgaa gggacaatta ttccaaaaat tattaatgtt ttcttggatg
     1561 atttttatat catcgtcaga gcattcaatc catcctttta tggaaacata tgatgccatg
     1621 tttaatctcc taaacctgtt ttaacaatgc cgccttttga ttcaatatat gacttaactt
     1681 gtgaatgaac accgtgcctg ttccgtacag caagcacagg ttggattaag attcgacaat
     1741 ctgtttggat tgacagaaag gtcgtctgaa aaatcgatta cggtttcaga cgaccttttg
     1801 ttgttgagtt ctcaacccaa cctatgcctg ccattaatgt tattcatcgt agtaaggttc
     1861 tgtcgaataa ttgtctttgc ccccggcaat gatagtaaca attttccctt ttgcttccca
     1921 agcttgtact cctatttcat caaactcata gacatatgtc ggataagatt catttgataa
     1981 ataatattta tcaacaccgt atgatttagg gtgatggaaa agctgtttaa aatcttcaaa
     2041 attcagacct attatattaa cgcccataaa atatagctcc tgataacaaa atatcgaaat
     2101 aattttgttt ttcttgacgg caattagtaa atttgagtcg ggagattcat aatattctgt
     2161 tctaaactca tcaggaggtt catacataaa agttcccagt atgtttttgt actgtgttat
     2221 atccgcacca aaacggaata ttcctacaga agtaaaaggt aaaaattcgg gagttttaac
     2281 gaccgcgtcg accatgctct tctccttttg cttttcgatt ggcatttttt gtaatatttt
     2341 tgattgtttt ctcaatcttt aagcgttcat ttttgggcat tccgggaata attttatttt
     2401 ttaattcagc aattcttgat tccgctgata tttgacttcg accgccgtct ccatgttttt
     2461 cattcttgga gcttcctgtt cttttaggcg gacaagaatt atgaacccaa accccttccg
     2521 tttccgcctg actgcccttg acgaagtaag tatgccagtc ggcaacggtc agattgtagg
     2581 ctttgagcgg tgttggtttg acaacgatgt tgcggacggt ttgggttttg ccactttcgg
     2641 ataacagcct gcttcccgct ttcaaatctt ccgctttaat ccatttgccg cccgaataaa
     2701 acggatggat gcggttggaa atcagggttt ggctgttgcc gatgccgtct gaaactttaa
     2761 tgtaaacggt ttcttgatac ggattgccgt atcgggcggt aacgggtttg tatcccgttt
     2821 ttccgcttgc ctcgtccttg gagaagacgc ggtcgccggt tcggatacgg gcaatggctt
     2881 tgtagccgtc tgccgttttg accaaggtgc tgccgtggaa ggagcaggtg taggattttt
     2941 gaagaactca gttctcaata tcctatttca aatgaaggaa atgccgtctg aaaattaaac
     3001 acggtttcag aagacattgt gattgttggt tttttaacct acgcttgcga aattaattcc
     3061 tttctcctga atactgcacc atttatttgg tttaactcca tagaagaatg attagaataa
     3121 atacattcaa agtcagcttc atcaaaatat tctccacaca tagaccataa ctctgtaatc
     3181 aaatattcca tatcttcatg gtcttctgtg atggcttctc ttgataaata atttttgatt
     3241 tttaaaaacc ttcttccttc ctcgtaatct acggtaatta acctgatatc tgagtgtatc
     3301 atgcccagca ttgctatatt cataccgact cgcagccagc ttggtaatat attcataact
     3361 attgcacctt tttaatggtt ttagggttag ccggaacaat gtgaactcca tttttacctg
     3421 aatgaatcgt accaaaattg gtacttaatc ctgatttccc atcgctgcct ataggatacc
     3481 caaaatcaac aacaggattt cctcttgctc ctttagaaat aactggataa gcacctgaat
     3541 gtattccgtt caacaattct tgaggattaa tgttttgatt taaagtactc ttaccttcaa
     3601 tgtagtttct atgtcctgaa atatgtttcc cttgagctcc atcatgaatt ttagtcccaa
     3661 tagattttgc tttaaaatca acattgcggg tttgaaacat ttctccatct acagactgag
     3721 ataatcttga agcagtgtta tgcttgtagg agtctgagaa atccccacta accgcagcct
     3781 tccccggttt tgccgccttt gtcaggtttt tgactttggc aaacggcagg acgttgacca
     3841 gggtttcgac ggtttcggcg gcattggggt tttcctgcat ccactggtca acggcttcgc
     3901 ccgcgctgat aaaggggtta agcgcgccgg cggcgacgcc gttgataaac tccatgctgt
     3961 tgccccagcg gtcgagcttg gcattgtgct cgaacatttt tctgttggct tcatcggcgc
     4021 ggtcggagaa attgctgccg aggttgttgt agttgtcgga tatgcgttgc cggatgctgc
     4081 gggtgtcggt cggattgagt ttgatgctgc gtgctgtgcc gttgacgtga taggtgtatt
     4141 cgtcgcgtgc gcccgtaggt ttggggtaat tgccgccctt cgggccgtcg taggcatcgg
     4201 cgggatggtg ctcgtgtcct tcccagttga gccggtacac ggtaaagcct tcgtcaacgt
     4261 tgccttgttc tttgcttgcg ctgtcggcgg cgtggttgtc gaagggggcg tgttcttcgt
     4321 gtccgtgtcc ggaaaagcgg gtgtggtagc cgattgtgcc gttgatgttt gcctgttgga
     4381 tgagcaggtt gcccatctgg tgggtatagt cttggatgac gttgattttg ccggtgcggt
     4441 cggaaacgct gccgcgcggg tcgccgaaga ggtggtattt gccgccgggt tcgtagtgct
     4501 gccgttgggc gttatcggta atgaacgggt cttgcgccaa gtccgccgcg agggcgggct
     4561 gtccgaaggc ggcgaccgct acggcgcagg cggcaaggag gtttgtcagt ctgcgcagcg
     4621 gtttcacggt ttatcctcct ttgcggcggc ggatgacttc gttgccgaca tcggggtttt
     4681 taccgttgtt ttgtttgaag tcgggacggt tttgggcggt tgtgtcgccg tagggggtga
     4741 tgtcggagaa atcgaccatc aggcggtctg aggctttgac ggttttgctg actttgtaag
     4801 ggccggtcca aagggcgtat tgttcttggt attgggattc gtaggcggcg gttttagggg
     4861 caatcagcag tttccggctg tcgcggtcga cggcgaaata ttcgagcttg gtttgggctt
     4921 taagggtttc ggcgttgtag aggtgcagtt cggtacggct gcggacggtg ccgaatacgt
     4981 cgacggttac gaatacgtcg gtgtcggcgt attcgggcgg tacgacttcg atgccgcgca
     5041 ggtagaagac ggtttggatg aggttggtca ggaaggaaac gtcgcggggg ttggcgagca
     5101 gggtttcgtt gcggtagtcg cccatgccgt tgacggacag tccggcggag cgttcgcctt
     5161 tgcgtccgct gtttctagtc agggcggcgg cgggggcgtt caaaagcgat gtggaagtgg
     5221 ttacgctgga gagcgcgtcg gatttggtgg tggcggtagt gtcgtaggcg gggtagctgt
     5281 attgggtggc actttcgggg ttgttgtggt agccaccgcg tatcagtgcg tcgatagagt
     5341 agcgtccgcc gcttatgttg cccgaacctt ggtcgcccat aacggagacg taaagggcgg
     5401 ctttgcgtcc tttcagggcg gacaaatcca tttctttgac ggcggcgcgg gacgatgcgg
     5461 cgacgagttc ttgttcgacg gcaaagcgtt tgccgccgcc gtgggcgggt atgccggtca
     5521 gtgtgccgca ggcggttagg acgaggggga tgaggaggag cagggttttc atggcagggt
     5581 ttgttttgtt ttgaacgagt ttgagtgtaa atggatttta agggtttgta aacgaaagag
     5641 gaaaaaatgc cgtctgagcg gcggaaatgg ctttcagacg gtatttgcgc tcaataataa
     5701 tatcccgcgc ccagaataca cggtttggat gcgccggttg ctttgtgcgg actaccggga
     5761 atgcgattaa tccaacacgc cgccaaccac gcaaatgcgg cggcttccac ccattgcgga
     5821 tcgaggttca ggtcggcggt gctgtgcagg gaaacgcgtg tgccgaaaca ttccgccaaa
     5881 tccgccatta aaacaggatt gcggataccg ccgccgcaaa tgtacatttg acgggcattt
     5941 gccgctgcgt gtgagacggc gtcgaaaacg gtttgcgcgg taaaacggga aagcgtccgc
     6001 aatacgtcgt atcggttttc gccgccgcca aggtaggttt cgagccaatt tagggcaaac
     6061 agttcgcgcc ccgtgctttt ggggtggggt cgtgcgaaat acgggtgggc gagcagcctg
     6121 tcgagcagtt gcggcaatat gttgccttgt gccgcctttg caccgttttt gtcgtaagga
     6181 agctgccagt gtgcctgcgt ccacgcgtcc atcagcatat tgcccggccc tgtgtcgaag
     6241 ccgaaggcgg gtgcgtcgga ggggagtacg ctgatgttgg caatcccgcc gatgttcagt
     6301 accgcgcgtg tttctttgtc atcgcggaac agggctttgt gaaaggcggg gacgagcggc
     6361 gcaccttgtc cgccggccgc aaggtcgcgg ctgcggaagt cgccgacggt aaaaatctga
     6421 gtcagttccg ccagcagcgg caaatcggca agctgtatgc tgtaaccgtg ttccggtgca
     6481 tgtcggacgg tttgcccgtg gcagccgagg gcggtaatgt cggacggcgc gaggttttga
     6541 ctgcacagca gttcggcggc ggtttgcgca tataggcggc tgagttcttg cgacaaaatc
     6601 ctgctgcggt gcagttcgtc tgtgcctgtg tcctgcaaat ccagcaattg gcggcgcagc
     6661 ctgtcgggat agggggtaaa ggcgtgccct tccgcgccca gccatttgcc gccgtccatc
     6721 cgtaccagca cggcatccgc cccatccata ctggttcccg acatgatgcc gatgtaaagc
     6781 tgtgtttcca tcatcactcc caaactggtg caaaacgcca ttttaacgtg tattgacgct
     6841 cgtataccga tttgccgccg cagtgtaaat aaagtgtaaa taaatgtttc aagacggatg
     6901 gaaaaatatt ataatgcgcc cgcaacatcc agtagtagaa gtgtcataca aaccgtttcc
     6961 ggcagcagtt ttgcattcgg tcaggtttgg gggtattcgg atgcggttca gaaggaagga
     7021 tgcgtctgcc atatcccgaa acggcagttc gaccggaggc agcagtacag tgtcggcaac
     7081 actcatgatt tccaccacat taaaggaaga ttgccatggc tcaaatccaa atgagcgcaa
     7141 atgttaaaac catcaacgcc gtctttgccg ccatgctggt aggtacagtc ggctatttta
     7201 tttattgggg cttgggttat acccattaca attacgccgc cttattcatt attgccacga
     7261 tgttcggcgt gtttatggcg ttcaacatcg gcggcaacga tgttgccaat tctttcggca
     7321 ccagcgtcgg tgcgggtacg ctgaccatcc cgcaggcttt gctgattgcg gcggtatttg
     7381 aggtcagcgg cgcggtcatc gcgggcggcg aggtaaccaa taccatacgc aaaggcatcg
     7441 tcgatttgaa gggtgttgat ttcgaaccca tacagtttgt gtttattatg atgtccgcgc
     7501 ttttggcggc ggcgttgtgg ctgttgtttg cctcgaaaaa agggcttccg gtatctacca
     7561 cccattccat tatcggcggc attgtcggca gcgcggtatg tatggcggta atgaacgatg
     7621 ccgcatcggg cgatttgata cgttggggca agctgggcgg tattggtgtt tcttgggtat
     7681 tgtcgcccgt gttgggcggc gcggtgtcct atttcctgtt ttcgcgcgtc aagaaaaacg
     7741 tcttagatta caacgcttgg gcggaaggca cgctcaaggg catcaagcag gaaaaaaagg
     7801 cctataaaga acggcaccgc ctgtttttcg agggtttgtc cgaagccgaa aaagtcgagt
     7861 acgccaccaa aatggcgcac gacgcgcaaa tttacgacga acccgaattc gatccgcaag
     7921 agctgcaatc ggagtattac cgcggtcttt atgcgttcga caaccgtaaa aacaatgtcg
     7981 attcctacaa agcgctgcat tcttggattc cctttatcgc ttcgttcggc gcgatgatga
     8041 tttccgctat gctgattttc aagggcttga aaaacctgca tttggggatg agcaacgtca
     8101 acagcttcct gaccatcttt atgataggcg cggcggtgtg gatggggacg tttgtgtttg
     8161 ccaaaagcct caagcgtaaa gacttgggca aatcgacctt tcagatgttt tcatggatgc
     8221 aggtctttac cgcctgcggc ttcgcattca gccacggtgc gaacgatatc gccaacgcca
     8281 tcggtccgtt tgccgcgatt atggatgttt tgcgtaccaa cagcgttgcc gcgcaaaatg
     8341 tcgtcccccc gattgcgatg ctgactttcg gcatcgcgct gattgtcggt ttgtggtttg
     8401 tcggtaaaga ggtgattaaa accgtcggta cgagtttggc cgaaatgcac cccgcctcgg
     8461 gttttaccgc cgaattgtcc gccgcatccg tcgtgatggg cgcgtcgctg atggggctgc
     8521 ccgtgtccag tacgcatatc ttggtcggcg cggtactcgg catcggtctg gtcaaccgca
     8581 atgccaactg gaaactgatg aagcccatcg gtttggcgtg ggtcattacc ctgcctgccg
     8641 ccgccgtatt gtcggttgtc tgctacttgg ttttacaggc agtattctga ttgtaaaata
     8701 ctgatgccgt ctgaacccgt gttcagacgg cattttgttg atggaatgtg cgggcttgtg
     8761 ccttatgcac aatctgttct gtcgggatat gccgtttggt atagtgatta acaaaaatca
     8821 ggacaaggcg acgaagccgc agacagtaca gctagtacgg caaggcgagg caacgctgta
     8881 ctggtttttg ttaatccact atatcttggt ttcggaacgg tcggacacaa aggtgcggaa
     8941 cgttatgata tgccgccgcc tgttcttgaa aacacttatc ctgccggcag caaaatgccg
     9001 tctgaaaaag cctttcagac ggcatttgta cgttagacgc aatcacaccg tttgcgaata
     9061 tttcgccttg gtttctttat ggcgcaggtg gtaatcgaac accatggcga tgttgcggat
     9121 gaggaagcgt cctttcgggg taacggtcag cccgtggctg ttcaggcgca ccaatcccaa
     9181 accggcgagt ttttccaaat ccgccagttc gtctttgaag tagcggtcga acgggatgcc
     9241 gaacatactt tcgtaaatcc ggtagtcgag cgcgaaacgg cacatcaaat cctgaatgat
     9301 gttgcggcgc aggatgtcgt cctgattgag ctgataaccg cgcatgatgg gcagtctgcc
     9361 ttcgtcgatg gcggcatagt aggcatcgat gtcgcgttcg ttttgggaat aggtgctgcc
     9421 gattttgccg atggacgaca cgccgatggc gaccaaatcg caatccgcgt aggtcgaata
     9481 gccttggaag ttgcgctgga ggaagccttc tttgagggcg atggagagtt cgtcgtcagg
     9541 tttggcgaaa tgatccatgc cgatgaagac gtagccgcgt tcggttaggg tttggacgca
     9601 gtattgcagc atatcgagct tctcttcgct gtcgggaacg gcggcggtat cgatgcggcg
     9661 ttgcggtttg aacacgtgcg gcaggtgggc gtagtgataa agggcgaggc ggtcgggatc
     9721 gagcgacaaa acggtatcga tggtggtttt gatgctttcc gaagtctggt gcggcaggcc
     9781 gtaaatcaaa tcgacgctga cggatttgaa ccccgcttcg cgcgccgcat cgatgacttc
     9841 tttggtttct tcgtaacttt ggatgcggtt gaccgccgcc tgcactttgg ggtcgaaatc
     9901 ctgaatgccg atgctcatgc ggttgaagcc gagtctgccg agcatgagga cggtgtcgcg
     9961 gctgactttg cgcgggtcga tttcgatgga gtattcgccg gtggggatta actcgaaatg
    10021 tttgcgtatc atgcggaaga cacgttcgat ctgttcgtcg ctcaaaaagg tcggcgtgcc
    10081 gccgccgaag tgcagttggg caagctggtg ccgtccgttc agatgtggag cgagcagttc
    10141 catttctttt tcaagatatt cgatgtaggc atcggcgcgg cttttgtctt tggtgatgat
    10201 tttgttgcag ccgcagtagt agcagatggt gttgcagaac ggaatgtgaa tgtaaaggga
    10261 aagcggtttg tttaacgcgc ccataccgcg caaatgtaaa gctttgatat attcgccttc
    10321 gcggaaaccg tcatggaaac ggtcggcggt agggtaggaa gtgtagcgcg ggccgctggc
    10381 gggcaggctg gcaatcagcg cgcggtcaaa ctcggggcgg tcatcgttta cattgtgatt
    10441 gttctgtatc tgaatgattt tcatggtgtg tgtgtgcgat tttatgatgt tagtcaaatt
    10501 ttggatagtt tggtagaatg ccacagtatg ataaacctgt cttgatatgt gtcaataagc
    10561 acatatagtg gattaaattt aaataaggac aaggcgaggc aacgccgtac tggtttaaat
    10621 ttaatccact ataatcatga tggggcaaag cgcacaaaaa ggtacggtat ggcttcgcat
    10681 aatactacac atcagatgaa aacgctgtgt tcttcctgtt ctttgcggga actctgcctg
    10741 cctgtcgggc tgctgcccaa cgagctcagc caactcgatg ccgtcatccg tcaaagccgc
    10801 cgcctgaaaa agggcgaata cctgttctgt gtcggcgaag cctttacctc gctctttgcc
    10861 atccgttcgg gcttcttcaa aacaaccgtc gccagtcagg acggccgcga tcaggtaacg
    10921 ggtttcttta tgtcgggcga actcatcggc atggacggca tctgttccca tgtgcacagt
    10981 tgcgacgcgg tcgccttgga agacagcgaa gtgtgcgaac tgccgtttac ccacatcgaa
    11041 gaactggggc aaaacatccc cagcctgcgt acgcacttct tccgcatgat gagccgtgaa
    11101 atcgtgcgcg accaaggtgt tatgctgctg ttgggcaata tgcgcgccga agagcggatt
    11161 gccgccttcc tgctgaacct ctcccaacgc ctttattccc gtggttttgc tgccaacgat
    11221 ttcatcctgc gcatgtctcg tgaagaaatc ggcagttatc tcgggctgaa acttgaaacc
    11281 gtcagccgca cattatccaa gttccatcag gaaggattga tttccgtcga gcataagcac
    11341 atcaaaatcc tcaatctgca ggtgttgaaa aaaatggtgt ccggctgctc gcacgccatt
    11401 tgattaaccc gtacgaacat ttcagacagc attctcaata aacacagggc agacgaaaac
    11461 atctgtcctg tttgttgtat ctgccgcaaa gtgccgtctg aaaaccggca gccgcctaaa
    11521 tcgaaaaatc ctcgctgatg ggcgtgtaca gaatcctatc caccttctcg cgtgtcaggt
    11581 gcggcgcaaa cgcttggata aagtcgtagg catatccgcg caaataagta tcgctgcgca
    11641 aagcaatcca cgtcggcgac ggctcgaaca ggtgtgccgc atccacaagc tgcaaatcgc
    11701 cgtccgtatc cgggttgtac gccattttcg ccatcagtcc cacgcccaaa cccaagcgca
    11761 cataagtctt caatgcgtcc gtatccgccg ccgccaatgc gacatccggc tgttccaaac
    11821 gggctttgga aaatgcccgc gcgatgctgc tgcccgcatt gaatgcaaat tcataagtaa
    11881 tcagcggaaa cctcgccaaa tcttcaatgc ggagggggtt tctgcattcg agcaaggggt
    11941 ggtcgttcgg tacgataacc gcatgagtcc agtcatagca gggaagtttt cccagttcgg
    12001 gatggtcgtc tatccgttcc gtaacaatcg ccaagtccgc ctcgcctgag gtaaccatac
    12061 gtgcgatggc ggcagggctc ccctgtttga tggtcaggtt gactttcgga tagcgtttca
    12121 caaaatcggc aacaatcaag ggtagggcat agcgtgcctg agtatgcgtc gtggcaaccg
    12181 tcagcgaacc gctgtcctgt ccggtaaact cgctgccgat atttttaatg ttctgtacat
    12241 cgcgtaaaat acgttccgca atatccaaaa ccaccttgcc cggctgcgag accgaaacca
    12301 cgcgcttgcc gctgcggata aaaatctgaa tgccgatttc ttcttccagc aatttgattt
    12361 gtttggagat gccgggttgc gaagtaaaca aggcttcggc cgcttcggaa acgttcaggt
    12421 tgtgctggta aacttctaag gcgtatttca attgttgtaa tttcatggcg ggtcggtgtg
    12481 ggtctgtgtc gggtggctga acattgttta taatttatca tattttcttg ccggtacggt
    12541 atggggcttt gccgttgtgt ttgttgtttt tgtgcaacgg caatcgtgcg atatggaaaa
    12601 aatcccccta aagtaatgac acggaattga tttttcggca tgatagacta tcaggaaaca
    12661 ggctgtttta cggttgtttt caggcgttga gtattgacag tcctccccct gcttctttat
    12721 agtggagact gaaatatccg atttgccgcc atgtttctac agcggcctgt atgttggcaa
    12781 ttcagcagtt gcttctgtat ctgctgtaca aatttaatga gggaataaaa tgaccaaaca
    12841 gctgaaatta agcgcattat tcgttgcatt gctcgcttcc ggcactgctg ttgcgggcga
    12901 ggcgtccgtt cagggttaca ccgtaagcgg ccagtcgaac gaaatcgtac gcaacaacta
    12961 tggcgaatgc tggaaaaacg cctactttga taaagcaagc caaggtcgcg tagaatgcgg
    13021 cgatgcggtt gctgcccccg aacccgagcc agaacccgaa cccgcacccg cgcctgtcgt
    13081 cgttgtggag caggctccgc aatatgttga tgaaaccatt tccctgtctg ccaaaaccct
    13141 gttcggtttc gataaggatt cattgcgcgc cgaagctcaa gacaacctga aagtattggc
    13201 gcaacgcctg agtcgaacca atgtccaatc tgtccgcgtc gaaggccata ccgactttat
    13261 gggttctgac aaatacaatc aggccctgtc cgaacgccgc gcatacgtag tggcaaacaa
    13321 cctggtcagc aacggcgtac ctgtttctag aatttctgct gttggcttgg gcgaatctca
    13381 agcgcaaatg actcaagttt gtgaagccga agttgccaaa ctgggtgcga aagtctctaa
    13441 agccaaaaaa cgtgaggctc tgattgcatg tatcgaacct gaccgccgtg tggatgtgaa
    13501 aatccgcagc atcgtaaccc gtcaggttgt gccggcacac aatcatcacc aacactaagg
    13561 ctaggcaata tcttgccgat gcatgaggtt agtggatttt gtaccaggta ctgttgcaat
    13621 attcgtgaaa cgtcggtcgg catcgatgat gtgaaacaaa cccccgcttt tgcggggttt
    13681 gtttttttgg gtggttttct gaaacggcta tcgtcagaat cggggtgcag gttcggattc
    13741 ggattcggat tcagattcag attcagattc agattcaggt ttgtgtccca ttgccgc
//
LOCUS       DAWHXZ010000030        13455 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000030 DAWHXZ010000000
VERSION     DAWHXZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.030"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(111..539)
                     /locus_tag="WIE35_000581"
     CDS             complement(111..539)
                     /locus_tag="WIE35_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ4153535.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(604..2352)
                     /locus_tag="WIE35_000582"
     CDS             complement(604..2352)
                     /locus_tag="WIE35_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ4153536.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2679..4112
                     /gene="ccoN"
                     /locus_tag="WIE35_000583"
     CDS             2679..4112
                     /gene="ccoN"
                     /locus_tag="WIE35_000583"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ4153537.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4139..4750
                     /gene="ccoO"
                     /locus_tag="WIE35_000584"
     CDS             4139..4750
                     /gene="ccoO"
                     /locus_tag="WIE35_000584"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ4153538.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4755..4925
                     /locus_tag="WIE35_000585"
     CDS             4755..4925
                     /locus_tag="WIE35_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ4153539.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4949..6046
                     /gene="ccoP"
                     /locus_tag="WIE35_000586"
     CDS             4949..6046
                     /gene="ccoP"
                     /locus_tag="WIE35_000586"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ4153540.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6086..6280
                     /locus_tag="WIE35_000587"
     CDS             6086..6280
                     /locus_tag="WIE35_000587"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ4153541.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6482..7891
                     /locus_tag="WIE35_000588"
     CDS             6482..7891
                     /locus_tag="WIE35_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ4153542.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYTMDEIHSDMMLGAAGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7993..11202
                     /gene="recC"
                     /locus_tag="WIE35_000589"
     CDS             7993..11202
                     /gene="recC"
                     /locus_tag="WIE35_000589"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ4153543.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEYCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWRQPATIGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFESQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQNFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKKEEAGEA"
     gene            complement(11270..12838)
                     /gene="mtrF"
                     /locus_tag="WIE35_000590"
     CDS             complement(11270..12838)
                     /gene="mtrF"
                     /locus_tag="WIE35_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ4153544.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13172..13384
                     /locus_tag="WIE35_000591"
     CDS             13172..13384
                     /locus_tag="WIE35_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153545.1"
                     /translation="MKNEIQKIMDKYNPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENIHAMIGEIKNAI"
BASE COUNT         3382 a         3233 c         3564 g         3276 t
ORIGIN      
        1 cactgtaaaa acggagaagg acagaggacg gcagtaaggt ttgccgtcct ctttttcaag
       61 ttgcggtgga atgccgcttg aaggtgtggt ttaaacgttt cggcttgtaa tcagacgcgg
      121 gcgttgtcca gccgctctct gaagttttcc aaatttacgc ctgccgcttg ggcgcggcgg
      181 atgatttcgt caaccggcat accttctgcc gcccggcgga agccccacag gagggagcag
      241 cgcgtaccgg tccggcaata ggcaaggacg ggatattcgg cttgtccgat gagttggcgg
      301 aaggtttcga catcgtgttt ttggatgtcg cgtgcggtaa cgggttggtg atggaatcca
      361 gtaacgcctg cttgttccag ccactgtttg atttgggcga agtcgggttg cgattcttct
      421 tcgcggtcgg ggcggttgca gatgacggtt ttgatgccca gttgcgcgat ttgttccgca
      481 tcggctttgg tcagttgcgg agaaatatag aggtgttcgt caagtttcag aatggccatt
      541 ttgtttcctt tactttgcgg ttgtttttca gacggcattt tgtgtgcaat gccgtctgaa
      601 agtttaaccg aacaaccagt tgtccaacag gttccggtct gtgatatgta ggatggcagt
      661 atccgctgcc gctgcctgaa ccgtcaagac ggtttgacgc aatatgccgg cacggaagaa
      721 gtggatattg atttttgcat tgacgtggta tcgggcccat tgtgcggtaa agtcggtgca
      781 ggcataaccg tctaaagcaa tgattttgtc ttgcgggcac agtgccgcag attccgcgct
      841 gccgccgttg aagacatggg tcaggacgat gtggtcggtg ttttgtttga aacgtgcgcc
      901 aaaatcgcct gccgacggga cggggcagat gtgttctgcg tatccgccgc cgtgttgtcg
      961 gggaagcggc aggaaggtca gtcccacgcc tgcggttgcc aggcattcgg caagcggcaa
     1021 atcttcggta ctgtataacg ctttttggaa aaaatcttcc aaatccaagc cggtaatttc
     1081 ctgacagcgg atttgccagt gtttttccgg aatacccgag tgtgtgtccc tccactcccg
     1141 atagagtttg tccattaacg tatcgagaga atgtctgccg ttgcttcggt tgcgtattat
     1201 cagatcaagg cacaatgcgg caagcgcgcc tttctggtag tagctgacga tggcgttggg
     1261 gctgttttca tccggtttgt aaaatttgtt ccacgcggta aaactcgatt ccgccaaggt
     1321 ctgcctcaaa cggccgcggg tttgttgtac gcgcgtaatg ccttgtgcca gcaggtttaa
     1381 ataagattcg ggcgagatgg tgcggctgcg tgccaaaaac aaatcgtcgt aataggatgt
     1441 aataccttcg aatgcccata gttgttcggt atagttttct ttgtcgaggt cataagggac
     1501 gaacgcggca ggtttgatgg atttgacgtt ccacgcgtga aaatattcgt gggagaaaag
     1561 tccgagcaat gtggtgtagg tatcgtcggc atcggtcata ccgtacggcg gaaggctgtg
     1621 gcggtcggcg agcagggcgg tgctgtcggt gtgttccaaa ccgccgtaaa tatggtcgcc
     1681 gacgtggagc aggaacaaat atttttgaaa cggggcaggg gaggaaaaca ccgccagttc
     1741 tgtttcgcag atttttttga tatccgaaac cagcctgttg cggtcgaaat cgggatatat
     1801 gccgcttaag gcaattgtgt gcggaatgcc tgccgcctca aaatctaaaa attcaatcaa
     1861 gcccatctcg acaggtcggt caatcaattc ggcataagat gccgcctgaa agacaaacct
     1921 ccctgtttcc ggcagcgttg tggcaatacg ccattcggac ggaatacccg tcaattccaa
     1981 gcggtgcggc agcgtttccg ttccttcgac tttcaaaaac aggcacgatc cgtcaaaaaa
     2041 accgcgttct gtcgtcagga aagaacctcg aaccgacaaa tcgaatgcat atacggtgta
     2101 gcggatttgc cactcgccgc gtacggcggc ggcatgccag cggtttttgg caatttgttc
     2161 gagcggcatg gacgtgccgt tacaggatgc atggatagaa gtgatgtggc gggaaaaatc
     2221 ccgaatcaga tagctgcccg gaacccagtt tggcaggctt atttctatag gaagatcatc
     2281 atcttgtgtg aatgtcagca ggatgtgcca ctcgtggtcg agaggggacg gggttatttt
     2341 ataatgaatc ataactatca aatatatcgt taaacgggat ggattaaata cctttaaaat
     2401 gcagcttaac ctacatcaat aaagtgccac aaaaagggaa aattggaaac aaaagcaatg
     2461 gcggtcgggt tgtaatttat tataacaata tgatttatat gtaataaaaa acagattaag
     2521 caaagggctc gtcggcggcg cgaatgtaat tccgcttggc gtaataaggc agatatgtta
     2581 aatttagaaa aaattacgct tgcccggatt gcaccttaaa caggctgcgg aagcggcagg
     2641 ataaaaatct ttattcccat aaattcatgg agactctcat ggacacacaa acttacaact
     2701 acaaagtggt gcgccagttc gccatcatga ctgtagtttg ggggattgtg ggtatgttgg
     2761 tcggcgttat cgtcgccgcc cagctttttg ctcctgccct cgacttgtct aatatcggac
     2821 cttggttcca cttcggccgc ctgcgtccgc tgcacaccaa tgcggttatt ttcgcatttg
     2881 gcggttgcgg cctgatcggc acatcatact acgttgtcca acgtacttgt aatacccgtc
     2941 ttttcggcgg ttggctgccg gcatttacct tttggggctg gcaggcggta atcgttgccg
     3001 ccgtcgtcag cttccctatg ggttggaccc aaggtaagga atatgccgaa ctggaatggc
     3061 cgatcgatat tctgattact ttggtttggg tggcttacgc catcgtattc ttcggtacga
     3121 ttgccaaacg taagattaaa catatttacg ttgccaactg gttctacggc ggctttattt
     3181 tggcggtcgc acttttacac atcgtcaaca atatcagcat cccggccggt ttgatgaagt
     3241 cataccccgt ctattccggt gcgattgatg ctatggttca atggtggtac gggcataatg
     3301 ccgtgggctt cttcctgact gccggcttct tgggtatgat gtactatttc gtacccaaac
     3361 aagcagcccg ccccgtttac tcctaccgcc tgtccgtcgt tcacttctgg gcgttgattt
     3421 tcacctatat gtgggcgggt ccgcaccatc ttcactacac tgcgctgcct gactggacgc
     3481 aatctttggg tatggttctg tctttgattc tgttcgcacc ctcttggggc ggtatgatta
     3541 acggcatcat gaccttgtcc ggcgcgtggg acaaactgcg tacagacccg attcttaaat
     3601 tcctgattgt atccttgtcc ttctacggta tgtctacctt tgaaggcccg atgatgtcga
     3661 ttaaaacggt caatgcattg agccactata cggactggac cgtcgcgcac gttcatgcgg
     3721 gtgcgttggg ctgggtaggc tttgtaacca tcggttccgt ctattacatg attccccgtc
     3781 tgttcggcaa agaacagatg cacagcacca agctggtaga agcacatttt tggattgcga
     3841 ccatcggcgt ggttctgtat atcgctgcca tgtggattgc cggtgtgatg cagggtttga
     3901 tgtggagttc tttgaacgat gacggtacgc tgacctactc gtttgtcgaa tccgtaaaac
     3961 gcaccatgcc ttactacgtg atccgttttg caggtggttt attgtatctg agtggtatgt
     4021 gcattatggc gtacaacgtg taccgcacag ccatcggtgg taaagcagtc gatgccgaaa
     4081 tccctgcggt ttcccaaaca cagcaccact aaaatactaa gaaagatagg ctaccaaaat
     4141 gaaattacaa caattggctg aagaaaaaat cggcgttctg attgtgttca cgctgcttgt
     4201 agtcagtgtc ggtctgttga ttgaagttgt gcccttggcc tttaccaagg cggcaacaca
     4261 gccggcaccg ggcgtgaagc cttacaatgc cttgcaggtt gccggacgcg atatttacat
     4321 ccgtgagggc tgttacaact gccactctca aatgattcgt ccgttccgtg cggaaaccga
     4381 gcgttacggt cattactctg ttgccggaga gtcggtttac gaccatcctt tccaatgggg
     4441 ttccaaacgt accggtcctg atttggcacg tgtaggcggc cgctattccg acgaatggca
     4501 ccgtatccac ctgctgaatc cccgtgatgt cgtgcctgag tccaatatgc cggcattccc
     4561 atggcttgca cgcaataaag tcgatgtcga tgcaaccgtt gccaacatga aggctttgcg
     4621 taaagtaggt actccttaca gtgatgagga aattgcgaaa gcgcctgagg ctttggcaaa
     4681 caaatccgag ctggatgctg tagtcgccta tctgcaagga ttgggtctgg ctttgaaaaa
     4741 cgtaaggtaa catcatggat attaacggta ttcgcgctct tttcacggta tggatcttta
     4801 tctgtttcct gttggtactc tatatcgtct tcaacaggcg gaataagaaa aactacgata
     4861 atgccgcaaa cagcattttt gatgaaaacc aagatgcgca agataagaaa agcgaaaacc
     4921 gttaatattg tgataacgga gcaaaacaat gaacacaaca tcccaattta ccagtaattt
     4981 ctggaatata tatattgcag ttattgtctt actgagcttt atcgctttgg cttggctgct
     5041 gctgtctcaa aatgttgtca aacgtccgaa gaagggcgaa gaagtacaaa ctacgggtca
     5101 tgagtgggac ggcattgccg aatacgacaa tccgctgccc cgctggtggt tttggctgtg
     5161 tgttttgacg tggctgttcg gtatcggtta tttggttatg tatccgggtg tcggcgacta
     5221 caaaggtctg ctgaaatgga ccagccataa ccaatatgaa aaagaggtca aaaaagccga
     5281 tgagcaatac ggcaaactgt atgccaagtt tgcggatatg ccgattgaaa aagtggcaaa
     5341 agaccctcaa gccaagcaaa tcgcccaaaa cctgtttaac acttattgta tccagtgcca
     5401 cggctctgat gctaaaggct ctaaaggttt tccgaatctg accgatagcg attggttgtg
     5461 gggcggtgat cccgataaaa tccacgagac catcgaaaaa ggccgtgttg cgactatgcc
     5521 tgcctggggt cctgctttgg gcgaagaagg cgtgaaagac gttgcccatt atgtgatgtc
     5581 cctttcaaaa cccgaaggtc agtatgatga ggaacgtgcc gcgcgcggac aagccttgtt
     5641 cagcggtccg cctgccaact gtttcacttg ccacggcgat aagggacaag gtatccaagg
     5701 tttgggtccg aacctgactg atgacgtgtg gttgtggggc ggtacgcaaa aatccattat
     5761 cgaaaccatt accaacggtc gcagcagcca aatgcccgct tggggacatt tcttggataa
     5821 agacaaactg catattatga ctgcttatgt atggggtctt tccgataaag acggtaaagc
     5881 tccggtgaaa aaagccgagc ctgcaccgac tcccgcaccg gcggcagaac ccgcagcctc
     5941 tgctcctgca gaagcagcac aagccgcgtc cgaagccaaa cctgccgcag cagaacctaa
     6001 agccgaggaa aaagctgcac ctgctgccaa agcggacggc aaataggttt atgaaaccgt
     6061 ttgtgccgcc tgccatggca atgcgattcc gggtattccc catgtaggca tcaaagccga
     6121 ttgggccgac cgcatcaaaa aaggcaagga cacgttgcac aaacacgcga tcgaaggttt
     6181 caatacgatg cccgcaaaag gcggtcgcgg cgatttgagc gatgatgagg ttaaagctgc
     6241 ggttgactat atggtcaacc agtcaggcgg caaattctaa cttgactgag tttccgatat
     6301 tatcggttat gaaaatccag attcagtatt gaatctggat tttttgtttt aacgggcatc
     6361 cggttttaaa cgcagactgc ctatttgttg taatgaattt tgactttagt cataaagaat
     6421 tagtctttat tgtttataat cgcgcatcat cacgatagcc ctattttcaa aaggacgatt
     6481 aatggataca caaatcaaaa ctgaagccga caatcagagc aaccggcgtt atctgaccgt
     6541 ttggcggtgg catttttatg ccggtctgct ggttatgcct tttcttaccc tgcttgccgt
     6601 tacgggtttg ggtatgctgc tgtttgccaa tattaccggt aaggagggcg agcggattca
     6661 tgttgtgccg caggcaacgg tacaacctct gtctgttcag gcggaagcgg cacgcagtgc
     6721 cgttaatccg gaaacttcgt ccgtcgttca gtatattgcg ccgcgtgccg atgatatggt
     6781 tgccgtgttc cgtgtcaaca atgagggcaa ggcaacgatg gtcgcggtcg atccttatac
     6841 ggcaaaagtg gtcagtacca tgccgcgcaa tcagggttgg tattacacga tggatgaaat
     6901 ccacagcgat atgatgctcg gtgcggcagg cgattatctt ttggaaacgg cagcatcgct
     6961 gacgattatc atgataatca gcggcttgta cctttggtgg gtgaaacggc gcggcatcaa
     7021 ggcgatgctg ctgccgtcaa aaggcagggc gcgttcttgg tggcggaatc tgcacggcac
     7081 gtttggaact tgggtgtcgt tgattttgct gttgttctgc ctgtcgggta ttgcttgggc
     7141 gggtatttgg ggcggcaagt tcgtacaggc ttggagtcag ttccctgccg gtaaatgggg
     7201 tgtcgaaccg aaccccgttt cagtcgtgcc gacccacggc gaggtattga atgacggcaa
     7261 ggttaaggaa gtgccgtggg ttttggagct tacgcctatg cctgtttcag ggacgactgt
     7321 gggcaaagac ggcattaacc ctgacgagcc gatgacattg gaaaccgtcg accgctttgc
     7381 gcgggaaatc ggtttcaaag ggcgttatca gttgaatttg cccaaaggcg aggacggcgt
     7441 atggactttg tcgcaggatt ctatgagtta cgacatgatc agcccgtttg ccgaccgcac
     7501 ggtacatatc gaccagtaca gcggcaaaat ccttgccgac atccgttttg acgattacaa
     7561 cccgttcggc aaatttatgg cggcaagcat tgcgctgcat atggggactc tgggctggtg
     7621 gagcgtgttg gcgaacgtct tgttctgcct tgccgtcatt tttatcggta tcagcggctg
     7681 cgtgatgtgg tggaaacgcc gtccgaccgg agcggtgggc atcgttccgc cggcgcagaa
     7741 agtcaagctg ccggtttggt ggatgatggc attgccgcta ttggcaatcg cactgctctt
     7801 cccgaccgca ctgcttgcca ttgccgtgat ttggctgttg gataccttgc tgctgtcgcg
     7861 gattcctgtt ttgaggagat ggtttaaatg acccgatgcc gtctgaagcg ttcagacggc
     7921 attttgtttg aaggcgggcg ggggaaaggc tatataatcc cgaatacttg accacagctt
     7981 ctgtttgaaa tcatgtttta tctgtatcaa tccaaccgtc ttgaaacgct ggcggcactg
     8041 tttgcccgca ttcaaaaagt caaaccgctg aaatcggttt tacagcccga acagattatt
     8101 gtgcagagtc aggggatgcg ccgctacctc aatacctgcc tcgcccgcga tttgggcgtg
     8161 gcggcgaatt tgtcgttcag cctgcccgcc ggtctgacgt ggaagctgat gaaaaaactg
     8221 attcccggta ttccggaact cagcccgttt gcgcctgaag tgatgcgctg gcggctgttg
     8281 gatttgttcc gcagcgaggc atttcggaat acggcagaat ttgaagatgt gaggaatgtg
     8341 ctgcaagact atctgggcag cggcgaatcg gcagattatc agcttgcggg acagcttgcg
     8401 gacatattcg accaatacct cgtgtaccgt cctcagtgga tagacgcttg gcagcagggc
     8461 aggcggctcg gtttgggcga cgacgaaatc tggcagtcca aactgtggcg ttacctcgac
     8521 gatggcaggc agagcgcgcc gcaccgtgtc gcgttgtggg aaaagctgtt ggaatcttta
     8581 agcagtgata agctgcccga gcgttatttc gtgttcggca tttccacgat ggcaccgatg
     8641 tatttgcagc ttttgcacaa gctgtccgaa tattgcgatg tgtttgtgtt cgcactcaat
     8701 ccgagcggga tgtattgggg caatgtcatc gaagcggcgc aaatcctcaa aggcggcggc
     8761 gatcccgatt taactcaggc agggcatccg ctgctcgcct cattgggcaa gcaggggcgg
     8821 gactttttcg actttttgaa cgaaatggaa atagaaggag aaacgccggt atttgaagaa
     8881 ggcgggcgcg atacgctttt gcacgccctg caaaccgata ttcaaaacct gaaaatgccg
     8941 tctgaaaatg tgggaagcgt caacacgggc gacggctcga tacgcatcgt ctccgcacac
     9001 agccccttgc gcgaattgca gatactcaaa gacaagctgt tgaaaattct gcatgaacat
     9061 cccgattggc agccgcatga tatcgccgta ttaaccccga acatcgaatc ctatacgcct
     9121 tttatcgaag ccgtgttcgg acaggcgcag cccggtgcgc aggcattgcc gtattccgtt
     9181 tcagacgtga aaatcagccg ccgacaaccg ctgtttcatg ctttgtcatg cctgttcgac
     9241 ttgttggaaa gccgatttga agtcgataaa gtgcttgtgc ttttagaaac cgcacccgtg
     9301 ttgcgccgtt tcggactgac tgaggacgat ttgccgcttt tgcacgatat ggttgccgat
     9361 ttgaacgttc actggggttt ggacggagaa atgcgcggcg gcacggatca gctgtttacc
     9421 tggaagcagg cggtagaacg catgatattg ggctggatgc tgcccgaagg cggcaacccg
     9481 atgtggcagg atgtcagcgc gtggtatgcc gacgtgaatc aaaccgccct gttcggacgt
     9541 tttgccgccc tcctcgaaac cctgtcggat attgtacgga tatggcggca gcccgcaacg
     9601 atcggcgaat gggtggcgcg ttgccgggat ttgcttgaaa cattgttcca agctgaagcc
     9661 gatgaccaaa agtcagtcca aaaccttgaa aacgaatggg tcaaatggca ggcggaaaca
     9721 gaattggcgc aattttccgg acagttgccg caacaaaccg tcatccgcca tatccgacgt
     9781 ttcctcgaca gcgaaagcga ggcaggcttt ttacgcggcg gcatcacctt ttgcagtatg
     9841 gtgccgatgc ggagcctgcc gttcaaagtc atctgcctgt tgggtttgaa cgacggagat
     9901 tttccccgca ataccaaagc cgccgtattc gacctgattg ccaaacatcc cgccaaaggc
     9961 gaccgcgccc gccgcgatga cgaccgctac ctcttcctcg aagccctcat cagcgcgcgc
    10021 gaaatcctct acctgtccta catcgggcgc gacatccgca aagacgaaga gcttgctccg
    10081 tcttcgctgt tgggcgaact catcgacaca gttgccgcta tggcgggcat cggtagccgc
    10141 caacttgcac aaaactggat agaacagcat ccgctgcaag ccttctcgcg ccgatatttt
    10201 caagaaggcg gacgttcaga cggcatattc ggcacgcgta ccgactacgc cactgcgctc
    10261 ggacaaacgc ccgaaccgcc gcaacccttt ttcgaccaac ccgtagaaaa cgccgaacct
    10321 gttgccgaaa tcggacagga cgaatttatc cgtttctggc gcaaccccgt caaagtatgg
    10381 cttcagcagc agcttgcgtg gagcgaaccc catatcggcg aagcctggga gcctgccgaa
    10441 cccttcgagt cgcaacacgc cgatcaaatc gccgaaacct acatcgaagc acggcgcgaa
    10501 ggacgggatt ttgcccaaac cgccgcccgc atcggggcgg aaagcctcct gccgtcggga
    10561 gagttgggca gactttggca gcagaacttc caaactgctg ccaaacaaat cgacacggcg
    10621 gttttaaaca gccccaaact gccaccgctt tcatatgcca taccgtcgga cgggcaaatc
    10681 ctgaaaggca gcttgggcaa tctgtaccgc tgcggacaag tgttttacgc ctacggcaaa
    10741 cccaacgcgc cgcaacgtat cgcttttctg ctggaacacc tgatattttg cgccgttatg
    10801 ccgtctgaag ccgaaacgcg gcaaaccttt atcgtccaat ccggagaaac cgaaatattg
    10861 gcggaaatcg cgcaagacag ggcattgcag ctattgtcgg aatggatggc gtttttcaat
    10921 atcgggcaaa accgcccgct gccgtttttt gccaagacct cgcttgccgc tgccgaagcg
    10981 tttgcccaaa aacaagattg ggaagccgcc ctgaaaaaag cccaaaccgc ctattacggc
    11041 aacaaagtca gcaaagggca gaaagactat accgaagtcg ccctcgtgtt cggcaacgca
    11101 agccaaaacc cactcgaaca acccctgttt gaaaacctcg cccgcctgct tgccgacacg
    11161 cttgccgcag cggaaaaaaa ggaagaggcc ggagaagcct gacgggatag aaaggcaaat
    11221 gccgtctgaa agcgtttaag catttcagac ggcattttgt ttatcgtgtt taaggtgcgg
    11281 gatagaatgt gggcgcaccg ggaccgacgg gcaggcccaa aacaaatacc caaatgcaga
    11341 ataaggcaat ccatgcaatt aagaagaaag cggaatacgg caacatcata gaaatcagcg
    11401 tacccacgcc cgcatctttt ttgtatttga tcaccgtcgc cataatcagc ccgaaataac
    11461 tcatcatcgg cgtaataata ttggtaacgg aatcaccgat gcggtaagcg gcttgaatga
    11521 cttcgggcgc gtagccggcc aacatcagca tagggacgaa aatcggcgca gttaccgccc
    11581 attgcgcgga ggcggagcct atcatcagat tgataaaagc acaaattaaa ataaaaccga
    11641 taaacaacac gctgccgccc aagccgactt cttttaagaa cgtcgcccct ttaacggcaa
    11701 tatattgccc aatattcgtc caattaaaaa atgcgacaaa ctgtgcggca aaaaagatga
    11761 tgaccaaata aagccccaga gtactcatcg attcggccat cgcattaacg acttcctgtt
    11821 cgccgcgcaa acttcgggtt acccggccat aaacaatgcc cggcagcgca aacaacaaga
    11881 aaataaaaac aacaatcgat tttaaaaacg gcgaaccgga aaccaatcct gtttcaggat
    11941 gacgcaaaat accgtcggca gggacgatgc tccaagccaa tagggcggat aaggcaacaa
    12001 acaccacgcc agcccaaatt aatcctttat attccaaagg cgtgatttca ttggaatgcc
    12061 gaatgtcttt ttcttcttgt gacaaatctg attgataagg gcccaattgc ggttcgacga
    12121 ttttttcagt aacaaaataa ccaatcaaag caatcacaaa cgtactggct accataaaaa
    12181 accagttggc ttcagggcct acgacgtagt cgggatggat gatttgcgcc gcctgttggg
    12241 tgatgcctgc caagagcgga tcgattgtgc ctaagaacag attggccgaa taaccgcccg
    12301 aaacgccggc gaaagccgca gccagaccgg caagcggatg gcggccgagg gaatgaaaga
    12361 tgatggcgga caaagggatt aggacgacat agcccaattc agaagcggta ttagataaaa
    12421 tccctgtaaa aacaaccata aaagtagtga gtttgcgtgg agattttgtg agcaataagc
    12481 gcattaatgc ggaaatcaag cccgattttt ccgcaatccc cacgcccaat aaagaaacca
    12541 acaccgttcc caacggcgcg aaaccggtga aatttttaac ggtatgcgtc aggattttga
    12601 tcaaaccgtc agcatcgagc aggctgacaa cgtgaatcaa accgtcatcg gcacgtcctt
    12661 tcgcaccaac agggcgcgga tcggggacgg atagtccgaa atacgcaccg gcggcagagg
    12721 caatcagcaa taacacaatg aaaataataa aaagcgtaac cgggtgcggc aacatattgc
    12781 ccagccattc gactgtgcgt aaaaatcgtc cgtcccgttg cgtatcggtt tgactcatct
    12841 gcttctcctt aaaaaaaata tattgagtgc attaaatttt tgaatgtaac atgaagtgtt
    12901 ttgtaagcaa actaaaataa tcgtatatgt atataaccgt aatagattaa aacagaaaat
    12961 gcccacatcg ttattttcat aaaggcggta atccgaaaac ttgacgttaa agccttatca
    13021 ggaatgactg gaactcaaaa aaacggattc ccgcctgcgc gggaatgacg ggattttcgg
    13081 tttttgtttg caagaatgcc ggagaagaat gcagtttaca ttttgaatga tattgttatt
    13141 aattgtaaat aaatattgga aggtaaatga tatgaaaaat gaaattcaaa aaatcatgga
    13201 caaatataat ccatggcatg aagatgattt tgagtcgtat gaagacattg ccaaagatgt
    13261 atcgctgatg acagataaaa cgttcattga acattatttg ttagaagttt attcagaaga
    13321 aaacggacat tttgaccaag agaatatcca tgcgatgata ggggaaatta aaaatgcaat
    13381 ttagtgaata tgagaccttt gcaaaaatag tctgttaacg aaatttgacg cataaaaatg
    13441 cgccaaaaaa ttttc
//
LOCUS       DAWHXZ010000031        13455 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000031 DAWHXZ010000000
VERSION     DAWHXZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.031"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(69..2045)
                     /locus_tag="WIE35_000592"
     CDS             complement(69..2045)
                     /locus_tag="WIE35_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ4153546.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVLTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLKGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="WIE35_000593"
     CDS             2194..3033
                     /locus_tag="WIE35_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ4153547.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALAQIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3234..4265
                     /gene="gap"
                     /locus_tag="WIE35_000594"
     CDS             3234..4265
                     /gene="gap"
                     /locus_tag="WIE35_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ4153548.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKDKLDGLAIRVPTVNVSLVDLNFQAARDTTIEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(4519..5244)
                     /locus_tag="WIE35_000595"
     CDS             complement(4519..5244)
                     /locus_tag="WIE35_000595"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ4153549.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGSV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(5314..5748)
                     /gene="fur"
                     /locus_tag="WIE35_000596"
     CDS             complement(5314..5748)
                     /gene="fur"
                     /locus_tag="WIE35_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ4153550.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            5967..6344
                     /locus_tag="WIE35_000597"
     CDS             5967..6344
                     /locus_tag="WIE35_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ4153551.1"
                     /translation="MNKTLILALSALLGLAACSVERVSLFPSYKLKIIQGNELEPRAV
                     ASLRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKDRSNLTVYFENGVLV
                     RTEGNALQNAAEALRVKQNADKQ"
     gene            6354..7163
                     /gene="dapB"
                     /locus_tag="WIE35_000598"
     CDS             6354..7163
                     /gene="dapB"
                     /locus_tag="WIE35_000598"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ4153552.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(7427..7837)
                     /locus_tag="WIE35_000599"
     CDS             complement(7427..7837)
                     /locus_tag="WIE35_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153553.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPMPNYSEWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEILDYGGA
                     LEVIGGRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            complement(7839..8084)
                     /locus_tag="WIE35_000600"
     CDS             complement(7839..8084)
                     /locus_tag="WIE35_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153554.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNEAELARVGLRL
                     KYNGLLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(8092..9264)
                     /gene="lpxB"
                     /locus_tag="WIE35_000601"
     CDS             complement(8092..9264)
                     /gene="lpxB"
                     /locus_tag="WIE35_000601"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ4153555.1"
                     /translation="MWGQTNMADKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAACFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(9312..10304)
                     /locus_tag="WIE35_000602"
     CDS             complement(9312..10304)
                     /locus_tag="WIE35_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ4153556.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            10799..>13455
                     /locus_tag="WIE35_000603"
     CDS             10799..>13455
                     /locus_tag="WIE35_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ4153557.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAERANEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRNE
                     RGQTALETAAVAETAVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPLI
                     ATPVQTASSAVSNGDALVYDAAEKIRRAAAILPEGATPKASAQEMPSETATFTAAAEQ
                     ARETAQTGGLVLIETDPAALKAWAAQPEV"
BASE COUNT         3382 a         4369 c         3142 g         2562 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgcctttc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg acggacggtt
      301 tcgccgtttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg tcagcaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gctgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccctgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccaa ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatgc cggcaacgaa tgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacgggacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccgaacac cagccgcctc
     1801 attgccctca gcttgggcag cgaaaactcc aagcctatac tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgttgcatga cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcataggtc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga agccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc agatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg tgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgtgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acacccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat atctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaaaac
     3061 cgtttcagac ggcattttat caagctctcc gtccgccgca cccgtgccgt ccgcatctta
     3121 ccgcccaccc ttccggccgc gccgttttca ataaaatatt aattacacgc cactacaaat
     3181 ttgctataat ccgccccgaa aatctaccca acccctcaac aaaggaacaa accatgggca
     3241 tcaaagtcgc catcaacggc tacggacgca tcggccgcca ggttttgcgc gccatctacg
     3301 attatcagat tcaagaccaa ctccaaatcg tcgccgtcaa cgccagcggc agccttgaaa
     3361 ccaacgccca cctcaccaaa ttcgacaccg tgcacggacg ctttgaagcc gacgtatccc
     3421 acgacggcgg caacctcatc gtcaacggcg acaaaatccc cttcttctcg acccgcaacc
     3481 ctgccgaact gccgtggaaa gaactcggtg tcgatttggt catggaatgc accggcgcgt
     3541 tcaccagcaa agaaaaagcc aaagttcatc tggaaagcgg cgcgaaaaaa gtcctcattt
     3601 ccgcaccggg cggcgacgat gtcgatgcaa ccgtcgtgta cggcgtgaac gacagcatcc
     3661 tgaccgccga catgaccgtc gtttccaacg cttcctgcac caccaactgc ctctcgccgg
     3721 ttgccaaagt gttgagcgaa agcgtcggca tcgtcaaagg cgcgatgacc accatccacg
     3781 cgctgaccaa cgaccaaacc gttaccgacg tgcgccacaa agacctgcgc cgcgcccgca
     3841 gcggcgtgga aaacatgatt ccgaccaaaa ctggcgcggc aaaagccgtc ggactggtac
     3901 tgcccgaatt gaaagacaaa ctcgacgggc ttgccatccg cgtgccgacc gtcaacgtat
     3961 cattggtaga tttgaacttc caagccgcgc gcgacacgac aatcgaagaa atcaacgcac
     4021 tgatgaaagc cgcctcggaa gcaggcgcgc tcaaaggcgt tttaggctac aacaccctgc
     4081 ccttggtttc catggacttc aaccacacca ccgaagccag ccacttcgac gcaacactga
     4141 ccaaagtcgt tgacggcaac atgattaaag tcttcgcatg gtatgacaac gaatggggct
     4201 tcagctgcca aatgctgaac accgcacgcc gtatgttcgg acttgaagtg cgcccgctca
     4261 aataagcaat aaaccgtcaa acaaaatgcc gtctgaaacc cgatgttttc aagttccaga
     4321 cggcattttt catttttggc agtgattttg cccggctgtc gtcatttcta attttatagt
     4381 ggattaacaa aaaccagtac ggcgttgcct cgccttgccg tactatttgt actgtctgcg
     4441 gcttcgtcgc cttgtcctga tttttgttaa tccactataa tccaccatat ttgaacctga
     4501 ccgaagcata agccgccatc aaagcccctt gcacgccact tcgcgccgcc cgatttcctc
     4561 tttcaacggc acggcgttca acatccgcag acgttcggca aaatccgcaa acggcagcag
     4621 ctccgaaccg aaacggcgca tatgttccgt gtcctgctgg cagtctatca gttccacacc
     4681 caaatccgcc aaaaacggca cggcgcaggc aaacgcgatt ttcgacgcat ccggttgcaa
     4741 cgcaaacatc gattcgccat aaaacacgct gccgatctgc acgccgtaaa agccgcccgc
     4801 caacctcgtt tcaccgcttt catcgggata atggcactcg aaagaatgcg cgtgtcccat
     4861 ttcgtgcagc ttcaaatatg ccgtctgaaa ctcgggcgca atccaagttc cgtcctgatt
     4921 cgggcgcgcc gctgccgcac aatgcgcgac cacttccgca aaacagccgt tgaccgcaac
     4981 ccgatagctg ccgttgcgca gcgttttcgc cagcgagcgc ggaacgtgca gacgttcggg
     5041 aacaatcacc gcacgcgggc cgaccgcata ccaaaaaaac caaccgtccc gagaaaacca
     5101 cggaaacacg ccgttccgat acgcctcgag cagccgcccc gcatccaaat cgccgctcac
     5161 gccgaccagc ccgtcgcacc gggccaaagc ataggcagga tcgggaaagg cataattgtc
     5221 aggggcaagc agcggaatac gcatggcagt tctccaaaaa cacagacggc atccaaaatc
     5281 atgaatgccg tctgaacaac aaaccgtccg gatttaacgt ttgcccttgg cctgacagtc
     5341 gctgcacacg ccgtacatat aaagcgcgtg atcgacgatg cggtagccgt tttcctcagc
     5401 gattttgtct tgcagggctt cgatttcggg attgtggaat tccgttacct cgccgcactt
     5461 cacgcagacg atatggtcgt ggtggtcgcc cttgtccaac tcataaaccg ccttgcccgt
     5521 ttcaaaatgg tggcgttgca gaatgcccgc ctgctcaaac tgggtcagca cgcggtaaat
     5581 cgtcgccaca ccgatttcca caccctcttc caacaaaatg cggtacacat cttccgcact
     5641 caaatgctct tccgcatgcg tctcgaacaa atccaaaatc ttcaaacgcg ggccggtaac
     5701 cttcagaccg ctgtctttca gttgtgcaat attgttgaat ttttccataa tattcaatac
     5761 ccctgtaaaa caatagacgt tataatacgc aatttcggcc tgtttgccca ctatcgcacc
     5821 atagcagttt gcaatagtaa aaccgcaccg gcggccgatg cgcggacagg gcggataccg
     5881 cttaatcgta tgattatcgt tcgcttttat aaaatattca agcagtgtta cactacccat
     5941 cccgatttgc acagaaaggc atttccgtga acaaaaccct catcctcgcc ctttccgccc
     6001 tcctcggcct tgccgcgtgc agcgtcgaac gcgtttcact gttcccctcg tacaaactca
     6061 aaatcataca gggcaacgaa ctcgaacctc gcgccgtcgc ctccctccgc cccggtatga
     6121 ccaaagacca agtcctgctc ctgctcggca gccccatact gcgcgacgca ttccataccg
     6181 accgctggga ctataccttc aacacctccc gcaacggcat catcaaagac cgaagcaatc
     6241 tgaccgtcta ttttgaaaac ggcgtgctcg tccgcaccga aggcaacgcc ctgcaaaatg
     6301 ccgccgaagc cctccgcgta aaacaaaacg cagacaaaca ataaggaaca cacatgacac
     6361 cgctcaaaat cgccatcgcc ggcgcaaacg gccgtatggg gcgcgtcctc gtcgaagccg
     6421 tcaacaacca tcccgacacc gtcctttccg gtgcgcttga acactcaggc tcagaagccc
     6481 tcgggctgga cgcaggctac gccgtcggac tcaaaaccgg catcgccatt tcagacgacg
     6541 ttgacgccgt tctcgcacaa agcgacgtac tcatcgactt cacccgcccc gagcccaccc
     6601 tcaaacacct gcaaaaatgc gttgaaaaac aagtcaacat catcatcggc acaacaggct
     6661 tcgacgatgc gggcaaagcc gctatccaca ctgccgccga acaaacaggc atcgttttcg
     6721 ccgccaactt cagcgtcggc gtcaacctca ccttccacat cctcgacacc gtcgcccgcg
     6781 tgttaaacga aggctacgac atcgaaatca ttgaaggcca ccaccgccac aaagtcgatg
     6841 cccccagcgg caccgcgtta cgcatgggcg aagtcatcgc cggcgcgctc ggcagagacc
     6901 tcaaacaatg cgccgtttac ggccgcgaag gccacaccgg tccgcgcgat ccgtcgacca
     6961 tcggctttgc caccgtccgc gcaggcgaca tcgtcggcga ccacaccgcc ctcttcgcca
     7021 ccgacggcga gcgcgtggaa atcacccaca aagccggcag ccgcatgacc tttgccgccg
     7081 gcgccgtccg cgccgcagtt tgggtaaacg gcaaaacggg tttgtacgat atgcaggacg
     7141 tactcgggct gaacaaccgt taacccccat acaaaatgcc gtctgaaaag atattgttca
     7201 gacggcattt tgccgacagg ctccgtatcg gcatatcaat gtttcaacac acgggacgat
     7261 acataaagcg tcgccctatg tgtcgtcctg atttggaagg gattacgccc ctcccaaata
     7321 aagtctgatt ctaccgcccc gaaggacaga tgtccgagtg gcggggtttc aaccgaaaag
     7381 gaaatacgat aaagttgctt gcgcggcatg acaagttacg gctcgtttaa ttaaaactct
     7441 cctgatctaa aaattctaac tctatttccc ggcaaactat atctatacta aaaccggtcg
     7501 gaagatcata cctaccgcct atcacttcca gcgcacctcc ataatccaag atttcatcat
     7561 cctgctttaa ttctccaagt tccaatacat ttttgatatt tttatcttgg ttcttcgtgg
     7621 cgtatccata gataaatttc agatagctga actgcttgaa tttaattttt acccattctg
     7681 aataattggg catagggatc tcttgaggaa agtccggata atccacattc tggacgatga
     7741 tatggaattc atcgtttttc aaagtatagt ttaatagagt gccctcatat atatacagaa
     7801 attgataatc tttttttgga atggtataag ttgtcatatt attttacctt ttcaaaaaca
     7861 atgagtttaa gattttttga gtccaagttg gttctactac tcttatgaaa tttcagttgt
     7921 ttaaactctt cctttaacag accattatat tttaacctta atcccactcg agccagttct
     7981 gcttcatttg gaattcctct taaatactta ttgtttgatg tgccattaat cacaatttct
     8041 cccccttttt ttgtaatacg tgctgcttcg ggaaaataat ccattaaccg ctcaacatcc
     8101 cgcctcttcc aaaaccgcgc gcgcggccaa atccgccgta tcttttttca acagcaggtg
     8161 caacgccctg aaatcctgtt gcagcgcggc aaccttatcg gggtgttcgt accagtccgc
     8221 caacgccgcc gccagttttt ccggttttgc ttcagattgc aataattccg gcacagcctc
     8281 cttacccaac aggatattcg gcaggccgac atgcggcact ttgattttgc gtttcacata
     8341 agcataagtc agcggcgaaa tcttgtagct gatgaccatc ggacgcttgc acaacgccac
     8401 ctccaaagtc gccgtaccgc tcgttaccag caccgcatcc gccgccctgc acactgtttc
     8461 agactgtctg tcgattaccg tcagcggcaa tccggcaaac tccggccgct gcaaaacttc
     8521 cgccaaacgc cgcttcgtcg cctccgttgc ggcaggcagc aggaagcatg cggcaggata
     8581 gcgttccaac aacaataatg ccgtctgaaa aaacaccggc gccatatagt cgatttcgct
     8641 gacgcggctg ccgggcagca gggcgaatac ggggatgccg gcatccacgc ccaaagtttg
     8701 ccgcgccgtt tcacggtcgt cttccaaggg cataagctgc gccatcggat gaccgacaaa
     8761 ctccgcacgt ccgcccgcat cgagataaag ctgcggctcc atcgggaaca ggcacaacac
     8821 gcggttgacc tgatgcacga ttttgcccac acgttcccgc cgccacgccc acaccgacgg
     8881 gctgacataa tgcacggtcg gaatccccga ccgtttcagc ttttccgcca cacccaaatt
     8941 aaaatcgggc gcatcgatac cgacaaagac atcaggtttc aacgacagca aatcccgtac
     9001 cagccccctg cgtatccgta aaatttccgg cagccgcctg accacttcga caaagccgcg
     9061 caccgccagc cgctcctgat cataaaggct ctcgaaacct tccgccttca tcagttcgcc
     9121 gccgataccg acaaaacgcg cctgcggaca acgcttgcgg atggcgcgta tcaggtgcgc
     9181 ccccaatagg tcgcccgacg cttcgccgac actgacggca atcaaagggc tttttttatc
     9241 agccatattc gtctgtcccc acatactttc acgtcccgat gccgtctgaa ggcttcagac
     9301 ggcatcgggg ttcacatttt cgtcccgttt tccaacatca ccgcaaactg cgccaagtcc
     9361 ggcggcagct ccgccttcaa caccagcggc tcgcccgtga gcggatggtt caagtgcagc
     9421 tcggacgcgt gcaaaaacat ccgcttcaaa cccaacttct gcaaacgacg gttcgcctga
     9481 taatcgccgt agcgttcgtc gcccgcaatc ggacagcctt gagattgcag gtggacgcgg
     9541 atttggtgcg tacgacccgt tttcagcgtt gcccgcacca aagtcaggtg cgacagcccg
     9601 acaccgtgca aaatgccgtc tgaaaaacgg cttaacacac ggaacaccgt gtgcgccgac
     9661 tgcccgtcct cgctgacgcg taccatcttt tcgccttgtg cgccggtata tttgaacagg
     9721 ggcagtttga catggaaatt gtcgtccggc agtttgccca cccccagcgc aaggtagatt
     9781 tttttggggt ggtcgttacg gatggcttcg tgaagtttga cgagcgcgct gcgtttcttc
     9841 gccaccatca acaagccgct cgtatccttg tccaaacgat gaaccaactc caaatacttc
     9901 gcctccggac gggcgcggcg caactgttcg ataacgccga aactcacgcc gctgccgccg
     9961 tggacggcaa cgccggacgg tttgtcgatg accaaaagcg catcgtcttc gtaaacaacg
    10021 tcaaacgcac gcgccggtac ggcggcacgc ctttcagacg gcatttcctt ctccgccacg
    10081 cgcacaggcg gaatccgcac cgtatccccc tccgcaatac ggctgtcggg tttgcagcgt
    10141 ttcttgttca accgcacctc gccggcgcgg ataatgcggt ggatatggct cttgggaaca
    10201 cccttgagga tttttatcag atagttatca aggcgttgac ccgcctcatg ttcggcaacc
    10261 cctatcaagc tgaccgaatc tttgcttatt tcgtgcgttt tcatctataa tccgaacatc
    10321 cgtttcagca aaaagcgcgc ccgccgcgcc cccctgaaac ggtttacttt aaaacggaat
    10381 tttatattaa aaacgcactc cgcaaagcat tttccctgcg cctgttccca gccggcaggc
    10441 gcagaacgta atcaagtttg aattgatttt gccgtttcgg cgcggaagtt aagacggcag
    10501 ccgggcccca agtcccaaac gcaaaccgcc caaaacacag gcatcacgat aacaagaaga
    10561 agccggcccc catttttccg accgcagcgt tcggaacgcg gcagacaaac cgccgcatcg
    10621 atctttatag ccttctgtat ccgacccttc cgcacacggt tctccggaac gtgcatccct
    10681 gcggattttc aactcaaaaa ctgattaccg gttttatccg aaaacatcgg aaaacccagc
    10741 cttacggaat gccgaaccgg accggcgcgc ccgaatgccg cccgtccacg aggtgatcat
    10801 gaaaagaatg ttattcaacg caacgcaggc cgaagagctg cgcgttgcca tcgtcgacgg
    10861 acaaaacctg ctggatttgg acatcgaaac gctgggcaaa gaacagcgca aaggcaatat
    10921 ctacaaaggc atcattaccc gcatcgagcc gtcgctggaa gcgtgtttcg tggattacgg
    10981 aaccgaccgc cacggtttct tgccgtttaa agaagtctcc cgctcatact tccaagacta
    11041 cgaaggcgga cgcgcgcgca tccaagacgt gctcaaagaa ggcatggaag tcatcgtcca
    11101 agtcgaaaaa gacgagcgcg gcaacaaagg cgcggcgctg accaccttca tcagcctcgc
    11161 cggacgctat ctggtattga tgccgaacaa cccgcgcggc ggcggcgtat cccgccgtat
    11221 cgaaggcgaa gaacgtcaag aactcaaagc cgctatggcg caactcgaca ttccgaacgg
    11281 catgagcatc atcgcccgta ccgccggcat cggccgcagc gcggaagagt tggaatggga
    11341 tttgaactat ctcaaacaac tctggcaggc gattgaagaa gcaggaaaag cgcatcacga
    11401 cccttacctg ctctttatgg aaagctcgct gctcatccgc gccatccgcg actatttccg
    11461 ccccgacatc ggcgaaattc tggtggacaa tcaagaagtt tacgaccaag ttgccgagtt
    11521 catgagctac gtcatgccgg gcaatatagg ccgtctgaaa ctctacgaag accacacgcc
    11581 gctgttttcc cgcttccaaa tcgaacacca aatcgaaagc gcgttttctc gcagcgtcag
    11641 cctgccttcc ggcggcgcaa tcgtcatcga ccacaccgaa gccctcgtct ccatcgatgt
    11701 caactccgca cgcgccactc gcggcgcaga cattgaagac accgcgttca aaaccaatat
    11761 ggaagccgcc gaagaagtcg cgcgccaaat gcgcctacga gacttgggcg gtttggtcgt
    11821 catcgacttc atcgacatgg aaaaccccaa acaccagcgc gatgtggaaa acgtcctgcg
    11881 cgacgcgctc aaaaaagacc gcgcccgcgt gcagatgggc aaactctccc gcttcggcct
    11941 tttggaattg agccgccaac gcctgaaacc agctttgggc gaaagcagcc acgtcgcctg
    12001 tccgcgctgc gccggcaccg gcgtgattcg gggcatcgaa tccaccgccc tgcacgtttt
    12061 gcgtatggtt caagaagaag caatgaaaga caacaccggc gaagtgcgcg cccaagtgcc
    12121 cgtcgatgtc gccaccttcc tgttaaacga aaaacgcgcc gagctgtttg cgatggaaga
    12181 gcgtttggat gtcaacgtcg tcctgattcc gaacatccac ctcgaaaacc cgcactacga
    12241 aatcaaccgc atccgcaccg acgacgtaga agaagacggc gaaccgagct acaagcgcgt
    12301 cgccgagccg gaagaagacg aatccgccaa acctttcggc ggcgaaaaag ccaaagccgc
    12361 ccgtcccgaa cccgccgtca aaggcgtgcg ccacaccagc cccgccccga ctgccgcccc
    12421 cgagaaaaaa acctcctggt gggacagctt caaagcatgg ctcaaacgca ttttcggcgg
    12481 cagcgaaacc caagccgcgc ccgctgccga aacctccgaa aaacgcagca cggcaaaccg
    12541 cagcggcagc cgcgccaaca accgccgcca aaacccgcgc cgcagcaaac gcgaaggcag
    12601 caaagtagaa gtccgcgaag tggcaggcaa aactgccgga caggaagcgc gtgccgacaa
    12661 agccgaaacg cgcaacaacg gcaaccgccg ccgcaacgaa cgcggcgacc gtgccgccga
    12721 acgcgccaac gaagcggaaa tccaagaccg caacatacag cctgccgcaa ccgttgcaga
    12781 tgccgcaccg tccgaaaccg aagtgcaaac cggcaaacgc cgccgcaacg gcagccgcaa
    12841 cgaacgcggc caaaccgcgc tggaaaccgc cgccgttgcc gaaacagccg ttcagacagc
    12901 ggaaaacacg ccgtccgaac cgcataccgc agaagacaaa ggcagcaagc ccaaatccga
    12961 acgcaaccgc cgcgaacgcg acagccgcga cgccaaagaa cgccgcgagc gcaacaatca
    13021 gcgcgaccgc cgtcaaaacg gcaaaaaacg caatatcccg tctgccgcca aaatcgagca
    13081 gtacctgaat attcacgaca ctgccgacaa agtccgttcc gccgccgcac acgttttcgg
    13141 agaaaccgac gcaaacgcgc cgattaccgt cagcattgcc gatccgctca tcgccacacc
    13201 cgttcagacg gcatcttccg ccgtttcaaa cggcgacgca ctggtttatg atgcggcaga
    13261 aaaaatccgc cgtgccgccg ccatcctgcc cgaaggcgcg acaccgaaag cctcagcaca
    13321 ggaaatgccg tctgaaaccg caacctttac ggctgcggcg gaacaggcac gggaaaccgc
    13381 acaaaccggc ggactcgtcc tgatcgaaac cgaccctgcc gcattgaaag catgggcggc
    13441 acaacccgaa gtcca
//
LOCUS       DAWHXZ010000032        13253 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000032 DAWHXZ010000000
VERSION     DAWHXZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13253
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.032"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(91..1452)
                     /gene="accC"
                     /locus_tag="WIE35_000604"
     CDS             complement(91..1452)
                     /gene="accC"
                     /locus_tag="WIE35_000604"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ4153558.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(1566..2018)
                     /locus_tag="WIE35_000605"
     CDS             complement(1566..2018)
                     /locus_tag="WIE35_000605"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ4153559.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQQ
                     VKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(2000..2257)
                     /locus_tag="WIE35_000606"
     CDS             complement(2000..2257)
                     /locus_tag="WIE35_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ4153560.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            2374..3414
                     /gene="queA"
                     /locus_tag="WIE35_000607"
     CDS             2374..3414
                     /gene="queA"
                     /locus_tag="WIE35_000607"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ4153561.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLHRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVADRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
     gene            complement(3888..4466)
                     /locus_tag="WIE35_000608"
     CDS             complement(3888..4466)
                     /locus_tag="WIE35_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ4153562.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRHRVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMT
                     RLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            4705..5604
                     /gene="crgA"
                     /locus_tag="WIE35_000609"
     CDS             4705..5604
                     /gene="crgA"
                     /locus_tag="WIE35_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ4153563.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(5832..9047)
                     /gene="carB"
                     /locus_tag="WIE35_000610"
     CDS             complement(5832..9047)
                     /gene="carB"
                     /locus_tag="WIE35_000610"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ4153564.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAMNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECEARPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEQLQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(9087..9698)
                     /locus_tag="WIE35_000611"
     CDS             complement(9087..9698)
                     /locus_tag="WIE35_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153565.1"
                     /translation="MINIIATWAMALTYIFLLLFIIVFIPIQLYLENIKKKKFKSRII
                     DILKNNHNNLDLNDIKQMTEAVNLNNFAARKIIKQLYYTDELNLNLVRQLQKEIQQEE
                     PFDGCSDELKPTLIGINELLETHGSESQKHLLTPIISELKELNQIKHDHKKMKTQSYI
                     AYIIAIISFFIGSISFYYTITAPSGKEIANTVVEQLKANQNQQ"
     gene            complement(10546..10935)
                     /locus_tag="WIE35_000612"
     CDS             complement(10546..10935)
                     /locus_tag="WIE35_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ4153566.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(11508..11633)
                     /locus_tag="WIE35_000613"
                     /pseudo
     CDS             complement(11508..11633)
                     /locus_tag="WIE35_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(11679..12812)
                     /gene="carA"
                     /locus_tag="WIE35_000614"
     CDS             complement(11679..12812)
                     /gene="carA"
                     /locus_tag="WIE35_000614"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ4153567.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWALGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIKAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2893 a         3327 c         3640 g         3393 t
ORIGIN      
        1 ctatatcgaa atcggtttca gacggcgcgg ggcaagggaa atgccgtctg aacgcggacg
       61 ggcggcttca gacggcatat cggcggcggt ttacttgtcc tgtttggctt tgcgatcttc
      121 cagccagtgt tccaaatagt ggatgctgac gccgcctttt tggaaacccg catcggcgaa
      181 caggtcgcgg tgaagcggcg tattggtttt gatgccggtt accgccagct cggcgagtgc
      241 gacgcgcatt ttcgccattg cctgttcacg cgttttgccg tgtacgcaga ttttgccgat
      301 caggctgtcg tagtacggtg ggatgcggta gccttggtaa atgtggctgt ccacgcggat
      361 accgaagccg ccgggcaggt ggcagctttc aatcgggccc gggctgggga tgaagttgta
      421 cgggtcttcg gcattgatgc ggcactcaaa cgcgtggcct tcgacttgaa tatccttttg
      481 tttgtattgc aaaggcaggc cggcggcgat gcggagttgc tcctgcacga tgtccacgcc
      541 ggtaatcagc tcggtaaccg gatgctcaac ctgaacgcgc gtattcatct cgataaagaa
      601 aaattcgcca tcttcgtaca ggaattcaaa cgtacctgcg ccacgatagc cgatgcgttt
      661 gcaggcgtcg gcacaggcgt tgccgatttt ggcgcgttct ttttcagtga tgaacggagc
      721 cggtgcttcc tcgatgactt tttggtggcg gcgttgcagc gaacagtcgc gctcggcaag
      781 gtagatggcg ttgccgtgtt cgtcggcaat cacttggatt tcgacgtggc gcggacgttg
      841 cagatagcgt tccatgtaaa ccatcgggtt gccgaatgcc gcgcctgctt ctgccttggt
      901 catttcgaca gattggagga ggtcttcttt tttctcgacc acgcgcatac cgcgaccgcc
      961 gccgccgcca gaggctttga taatgacggg ataaccgact ttatcggcga ttttgaggat
     1021 ttcttcgccg tcgtcgggca atgcgccgtc agaaccgggg acgcagggta cgcctgccgc
     1081 tatcatcgcg tgtttggcgg agactttgtc gcccatcagg cggatggtgt cgggtttcgg
     1141 gccgataaag gtaaagccgg actgctcgac ctgttcggcg aaatcggcgt tttcggcaag
     1201 gaaaccgtag cccggatgga cagcgtccgc gcagcttact tcggcggcgg cgataatggc
     1261 ggggacgtta aggtagcttt gcgcggaagc ggcagggccg atgcacacgg attcgtcggc
     1321 gagtttgacg tgcaggctgt ctttgtcggc ctcggaatgc acggcgacgg tggcaatgcc
     1381 catttcgcgg caggcacgga gtacgcgtaa tgcgatttcg cctcggttgg cgattaaaac
     1441 ttttttcagc atgatgactg ttcctgtggt tccggcaacc cgtccgtaaa aaaaaaggtg
     1501 cgggaacgcc gaagggggaa agcatttcag acggcatcgg aagtttatgc cgtctgaaaa
     1561 caggattatc cgatgatgaa gagcggttcg ccgaattcga cgggcgtacc gttttcgacc
     1621 agaatttctt tgaccgtgcc ggatttttcg gcttcgattt cgttcatcag cttcatcgct
     1681 tcgatgatgc acagcgtgtc gccggcttta acctgttggc cgacttcgac aaaaggcgcg
     1741 gcattcgggc cgggtgcgcg gtagaacgtg ccgaccatag gcgatttttg cgcgtcggac
     1801 aaatcgcggg cggcaggtgc ggcgggcgcg gatgccgcaa cgggtgcggc ggcaggcgtt
     1861 acggccggcg cggcggcagg tacgggcgcg gcgtaaacgg gtgcggcggc gatggttcgg
     1921 gtgatgcgga ctttttcctc gccttcggtt acttcgattt cggcgatacc cgattcttca
     1981 accaaatcaa tcagtttttt taatttgcgc aaatccattt ctgttccttt aaaggctgcc
     2041 ggtgttccgg cgggttgttg cgttggtttt tcagaaaaca gccggcttgc ggcaacggcg
     2101 caaaccgggc ggaatggtgt tcggatacgg ttttcctgtg gtggaaaaca accgtgagaa
     2161 tgtgtttatt ttcccgaagt tgtcggtaaa tgtccagtaa aatatcaaaa aacggcggtt
     2221 tttgggggaa atgtgcagga agcttgattt tgcgcacaag atgccccggg tcaagagtgt
     2281 ttattctaat ctgttggttt ttcggacaaa gatgccgtct gaaaaagggc taaagtgcgt
     2341 ataatggcgg cttgcccgaa cgagagtgta aaaatggata tttcagattt tgattttacc
     2401 ctgcccgaaa agctgattgc ccagcatccg cccgaagtgc gcggcagcag ccgccttttg
     2461 gttgcgttgc ccgatatgcc gctgcaagac cgggtgttcg gcgatttgcc ggattatgtc
     2521 gaggcgggcg acgttttggt gttcaacaac accaaagtca tgaaggcgcg gctgtttggg
     2581 cagaaagaca gcggcggcag gatcgaagcc ctgattgagc gcgtgttgga caaccatacc
     2641 gcactggcgc acatccgttc gtccaagtcc cccaagcccg gtatggggct ggtgtttgaa
     2701 ggcggcatcc gtgccgtgat ggtcgggcgt gagggcgaac tgttctgcct gcgttttgaa
     2761 ggcggtcaaa ccgtttacga acttttggaa cagaacggcc atttgcctct gccgccttat
     2821 atcgaacgtg ccgccgacgc ggacgacgac agccgttatc aaaccgttta tgccaaatat
     2881 cagggcgcgg tcgccgcgcc gacggcgggc ctgcatttta cggaagaact tttgcaccgt
     2941 ctgaaagaca aaggcgcggt aaccgcagaa gtaactctgc acgtcggtgc ggggacattc
     3001 caacccgtgc gtgtggaaaa aatcgaagaa cacaaaatgc acagcgaatg gtttgaagtg
     3061 ccgtctgaaa ccgccgccgc cgttgaggcg gcaaaagccc gggggaacaa agtctgggcg
     3121 gtcggcacga cttccatgcg cgccctcgaa tctgccgcgc gtgcgacggg tcgtctgaaa
     3181 gcggggcagg gcgacaccga tattttcatc acgccgggct accgttttaa tgttgccgac
     3241 aggctggtta ccaatttcca tctgccgaaa tcgacgctgc tgatgcttgt cagcgcgttt
     3301 tcgggtatgg gtcatatccg cgccgcgtat cgttatgcgg tcgaacgcga ataccgtttc
     3361 ttcagctacg gcgatgcgat ggttttgggg cggaacgaag gggtggtacg ttagaacagc
     3421 ctcaagtcta ttgggggaag acaagtgccg tctgaacgga tgttcggacg gcattctttt
     3481 gttgatttga atgaaaggaa ctgcatgaca tatagtggat taaatttaaa tcaggacaag
     3541 gcaacgaagc cgtagacagt acaaatagta cggcaaggcg aggcaacgct gtactggttt
     3601 ttgttaatcc actatagatt caggcgcgag aggcgagaaa ctcagtcatt gttgatgacg
     3661 cgcaatacgt taaaattcat tccgttttgg tcgtataaac atattacata cgccattttt
     3721 ttgtattcaa ggctgcctga aaacagtggg caagaatatc taacccaacg aaaagctgaa
     3781 aaagcaacaa aaaataatct gcaccttagt cggttggtta gtccaacctt aggggtgcag
     3841 accattcaat ctttcaggct gcctttgttt gtttataaga taaattttta gccgaacact
     3901 ttttccaagt gtgcctgata atctgccaag tatttttcca cttgtggatt tttaaccaca
     3961 tcgttacata agaatgtcgg caggcgggtc atacccaaaa actcgttggc tttgtggaag
     4021 tgcatataca aaacatcaac gcctttgcct tcaaagaaat cgccttcgcg ggtaaacgcc
     4081 tcaatcggcg cattccaagt cagtgaaagc atatgttttt tgccttgcaa caagccgcct
     4141 gtgccgtagc cctcagtcgg attgacgcgg tgtctgccgt cgctttggta gagtttgccg
     4201 tgtccagcgg ttaatacttc gtctatgtat tttttcactg tccaaggctc gtgcatccac
     4261 cagcccggca tctgccaaat cacagcatcc atccaaacga atttttcgat ttctgcctca
     4321 acatcatagc cggcatcaat cacggtttct tgaacattgt gtccgagcgc ggtcaaaact
     4381 tctttcgctt ttttgtgaag cgtgcggttt aacccgccgt gagaatgtcc gaacgctttg
     4441 ccgccgtcta ataataaaat attcatttgt tacctcgttt gtgaattgat gaatgtattg
     4501 tgccgtttta ctttataatt taaaagtgca aaaataagaa aacacttttg cgtcaaatga
     4561 aataatcaga tgttccaaca cacgggatgg cacataaagc accgccctgt gtgccgtcct
     4621 gattcggaag gggttacacc cctcccaaat aaagtctgat cctgccgccc taaagggcgg
     4681 ggttccaacc gaaaaggaaa cacgatgaaa accaattcag aagaactgac cgtatttgtt
     4741 caagtggtgg aaagcggcag cttcagccgt gcggcggagc agttggcgat ggcaaattct
     4801 gccgtaagcc gcatcgtcaa acggctggag gaaaagttgg gtgtgaacct gctcaaccgc
     4861 accacgcggc aactcagtct gacggaagaa ggcgcgcaat atttccgccg cgcgcagaga
     4921 atcctgcaag aaatggcagc ggcggaaacc gaaatgctgg cagtgcacga aataccgcaa
     4981 ggcgtgttgc gcgtggattc cgcgatgccg atggtgctgc atctgctggc gccgctggca
     5041 acaaaattca acgaacgcta tccgcatatc cgactttcgc tcgtttcttc cgaaggctat
     5101 atcaatctga ttgaacgcaa agtcgatatt gccttacggg ccggagaatt ggacgattcc
     5161 gggctgcgtg cacgccatct gtttgacagc cgcttccgcg taatcgccag tcctgaatac
     5221 ctggcaaaac acggcacgcc gcaatctaca gaagagcttg ccggccacca atgtttaggc
     5281 ttcaccgaac ccggttctct aaatacatgg gcggttttag atgcgcaggg aaatccctat
     5341 aagatttcac cgcactttac cgccagcagc ggtgaaatct tacgctcgtt gtgcctttca
     5401 ggttgcggta ttgcttgctt atcagatttt ttggttgaca acgacatcgc tgaaggaaag
     5461 ttaattcccc tgctcgccga acaaacctcc gataaaacac acccctttaa tgctgtttat
     5521 tacagcgata aagccgtcaa tctccgctta cgcgtatttt tggatttttt agtggaggaa
     5581 ctgggaaaca atctctgtgg ataattctat aaaaacctgc cataaaattg tttttactca
     5641 cttaataact ttgagaaaaa ataaataaga tgatgtatag ccgcggcaat ggcagtctat
     5701 atggggagcg tgatagggct gttccttatg tacggcttgt gtgtggcaac atcggctttc
     5761 caaatgacaa aatgccgtct gaaaccgtaa aaaaaaaggt ttcagacggc attttcaacc
     5821 tgattcaggc atcagttgcg gtttttcaaa cgcccgtgca gttcttgaac gctgtacacg
     5881 cccaagtggt cgcggctttt cgcgccttcg ctcatcgctt cgccgccggc ggtggtggtg
     5941 tattgcggca cgcgttgctg caatgcgctt tggcggatga tgtggctgtc ggacacggat
     6001 tgcggatcgc tggaaacggt attcacgacc agtgcgattt caccgttttt cagcgcgtcg
     6061 ccgatgtgcg ggcggccttc ggggacttta ttgatggttt ggacaatcag cccgtgttcg
     6121 gtcaggtatt gcgccgtgcc gcgcgtggcg cagatgccgt agcctaaggc ttggaagttt
     6181 ttggcggttt taatgacgcg ttctttgtct tcttcgcgca cggagaggaa gattttgccg
     6241 gtcgggttga ggcgttcgcc tgcgccgagt tgggctttgt agtaggcttc gccgaagctt
     6301 gcgcccacgc ccatgacttc gccggtggag cgcatttccg gacccaaaat cgtatccacg
     6361 cccgggaatt tgatgaaggg gaacacggct tctttaacgg catagaaatc agggacgact
     6421 tctttttcca cgccttgttc tttcagggaa atgcctgcca tacagcgtgc gccgactttg
     6481 gcgagcggca cgccggtggc tttggagacg aaggggacgg tgcggctggc gcgcgggttc
     6541 acttccaata cgaacactac gccgtcctgc acggcaaact gcacgttcat cagtccgacc
     6601 acgcccagcg cgtacgccat cgctttggtt tggcggcgga tttcgtcttg gatttcttcg
     6661 cttaaggagt agggcggcag cgagcagccg gagtcgccgg agtggatgcc cgcctgttcg
     6721 acgtgctgca tgatgccgcc gataaccaca tctttgccgt ctgaaacgca gtccacatcg
     6781 acttcaatcg cgttgttcag gaagaagtcg agcaacacgg ggctgtcttc ggaaacctgc
     6841 acggcttcgc gcatatattt ttgcaactgc tcggcagagt ggacaatctg catcgcgcgt
     6901 ccgcccaaaa cataagacgg gcgcacgacc agcggataac cgatttcttc ggctttgacg
     6961 agtgcttctt cttcgttgtg ggcgatgcgg ttgggcggct ggcgcaggcc taagtcgttc
     7021 aacacttttt ggaagcgttc gcggtcttcg gcggcatcga tgctgtcggc ggacgtgccg
     7081 atgatgttca cgccgttttc aaccagcgcg ttggcgagtt tgagcggggt ttgaccgccg
     7141 taatgcacaa tcacgcccca cgggttttcg gtgcggacga tttccaacac gtcttccaat
     7201 gtcagcggct cgaaatagag gcggtcgctg gtgtcgaagt cggtggacac ggtttctggg
     7261 ttgcagttga ccataatcgt ttcaaaaccg gattcgcgca gggcgagcgc ggcgtgaacg
     7321 cagcagtagt caaactcgat gccctgaccg atgcggttcg ggccgccgcc gagaatcatc
     7381 acttttttgc ggtcggaagg acgcgcttcg cattcctctt cgtaagtgga atagaggtag
     7441 gcggtttcgg tggcgaactc ggcggcacag gtgtcgacgc gtttgtaaac cggatgcagc
     7501 ttcagcgcgt agcggtgttc gcgaacttct ttttcgctta cgttcaacaa ttgtgccaaa
     7561 cgtttgtcgg agaagccttt gcgtttcaga cggcgtaggg cggcgaaatc caaatcactc
     7621 aaaatgccgt ctgaaaccgc tttttcttcc ttcatcaagt cttcgatttg cgccaggaac
     7681 caagggtcga tggcgcagat ttcgtggatt tcttccagcg tgaagccggc gcggaacgcg
     7741 tctgccacaa acagcatacg ctcagggccg gggttcgcca gttcgcggcg gatttccgct
     7801 ttgtcggagc ttctcggatt gaagccgcac aagccggttt ccaagccgcg caaggctttt
     7861 tggaagcttt cctgaatcgt gcggcccatc gccatcactt cacccaccga tttcatctgt
     7921 gtggtcaggc ggtcgtctgc ggcagggaat ttttcaaacg cgaaacgcgg gattttggtt
     7981 acgacatagt cgatggacgg ctcgaacgac gcgggggttt tgccgccggt gatgtcgttg
     8041 cgcaactcgt ccagcgtaaa gccgaccgcc agcttggccg ccaccttcgc aatcgggaag
     8101 cccgtcgctt tggaagccag cgcggacgaa cggctcacgc gcgggttcat ctcaatcaca
     8161 atcatctcgc cgttttcagg gttcatcgca aactgcacgt ttgagccgcc cgtgtccacg
     8221 ccgatttcgc gcaataccgc caacgaagcg ttgcgcatga tttggtattc cttgtccgtc
     8281 agcgtttgcg ccggcgcgac cgtaatcgag tcgcccgtat gcacgcccat cgggtcgaag
     8341 ttttcaatcg agcagatgat gatgcagttg tcgttcttgt cgcgcactac ttccatctcg
     8401 tactctttcc agccgagaac ggactgctca atcagcagct cgtgcgtggg cgaggcatcg
     8461 aaaccgcgtt cgcaaatcgc caaaaactcg tctttattgt aggcaatgcc gccgcccgaa
     8521 ccgcccatcg tgaaagaagg acgaatcagg gtagggaagc cgacctgttc ctgcgccgcc
     8581 aaagcttcgt tcattgtgtg gcagacaaaa gatttcgggc aagacaaacc gattttttcc
     8641 atcgcctctt taaagcggcc gcggtcttct gctttgtcga tggcgtcttc cgtcgcgccg
     8701 attaattcaa cattgtattt cgccagcacg ccgttgcgcg ccaaatccag cgcacagttc
     8761 agcgcagttt gaccgcccat ggtaggcaga atcgcatcgg gccgctcttt ggcaataatt
     8821 ttttccaccg tctgccacat aatcggctcg atgtaggtaa catccgccat ttcggggtcg
     8881 gtcataatcg tggcggggtt ggaattcacc aaaatgactt tatagccttc ttcacgcaag
     8941 gctttgcagg cctgtgcgcc cgaatagtca aattcgcagg cctgaccgat aacgataggg
     9001 ccggcgccga tgataaggat ggattttagg tcggtacgtt tgggcatgat gggtctttct
     9061 atgaaatcaa atgtttagct aaaaatttat tgctgatttt gattggcttt taattgctct
     9121 acgacagtat tggcaatctc ttttcctgat ggggctgtaa ttgtgtaata aaagctaata
     9181 ctgcctatga agaaactgat aatggctatg atataggcta tataactttg tgttttcatt
     9241 tttttgtgat catgtttgat ctgatttaat tcttttaatt cagaaataat tggagttaat
     9301 aaatgttttt gactttcaga tccatgcgtt tccaaaagtt catttattcc tattagtgtt
     9361 ggttttagtt cgtctgagca gccatcaaaa ggttcttctt gttggatttc cttttgtaac
     9421 tgtctaacta aatttaaatt taattcatca gtgtaatata gttgcttaat aatttttctg
     9481 gcagcaaaat tattaaggtt tactgcttct gtcatttgtt ttatatcatt aagatctagg
     9541 ttattgtgat tattttttaa tatatcaatt attctagatt taaatttctt tttctttata
     9601 ttctctaaat atagctgaat tggaataaaa acaattataa agagcaaaag aaatatatat
     9661 gttagtgcca tagcccatgt ggcaattata tttatcattt atcaatatct tccatttggt
     9721 ttatatattg tctcataata agcaagccgt agctcgcatg cagggtgtgt gcggtacgca
     9781 cgcgttctcc attttccctg caaccccaac ccaccgtgtg cgtgccttgc ggcacacacc
     9841 ctaccgatgg gttcaaaggt cgtctgaaaa aacgctcgcg gcgtttgttc catctgcctt
     9901 gcgataaagg ctgcctgaaa aattcccgct gttgatattt cgggcattct ttctcaaacc
     9961 gcctgcaacc gcaaaatccg ctgcaacctg ccccctcccc cgtgggggag ggttggggag
    10021 agggcattcc ccgaatttcg gcaacctttc ccaacaactc aaccaccgaa atacaagcct
    10081 tgtggcttgt tgccctctct ctagctctct cccacgggag agaggacgga gtggctgtag
    10141 ggctaagggt tctgcaagga aacaggttgt gcaggttgct ttttgctttt cagacgacct
    10201 gaaatcaacg gtaaatccaa gtcgcttttc aaaccgcctg caaccgcaaa atctgctgaa
    10261 acctactccc tcccccgtgg gggagggctg gggagagggc attctccgaa tttcggcaac
    10321 ctttcctaac actctaaccg ccaagacaca agccttgcgg cttgttgccc tctctctagc
    10381 tctctcccac gggagagagg atggggtggc tgttggggct aaggtttctg taaactaatc
    10441 aggttatgca ggctgctttt tgtttttcag acggccttta taaatgctca attcagtcgg
    10501 ctttatccta ttattttcaa taatccaatc aaaatcagcc atctgctatc gtgcagcttg
    10561 cttttccaat tcttgcaaca cgagcaggat ttctgtcagc acatcgtttg tctgctgtaa
    10621 aatctcgtga ttccaaaacc gcagtacggt aaagcccaag ctgttgagat atgccgtccg
    10681 cgcgtggtcg tatgcggctt gttctgtgtg ctgccctccg tctgcttcga caatcaactt
    10741 gggcgttacg cacataaaat caacaatata gtttcccatc ggctgctggc ggcggaattt
    10801 atagccgttc agacggcctg cccgcaggtg ctgccacaat tttgcttccg cctcgctcat
    10861 ttcttggcgc atggctttgg cgcgttggcg caagatgggg ttgtcggtgg tcaagagttt
    10921 ctcgtgcggg ttcattttgt tgtcgtgctg tttgctgccc tctctctaac tctctcccac
    10981 ggggagagag gacggggcgg ttgttggggt taaaggggct gtaaggaaaa caagtcgttc
    11041 aggccacttt ttacttttca gacgacctgc aatcaacggt tgtaaatcca agttgctttt
    11101 caaaccgcct gtaaccgcaa aacttgctgc aacctacccc ctcccccgtg ggggagggct
    11161 ggggagaggg cattctccga atcgcggcaa tctttcccta attccctaac tgcctcaata
    11221 caagcctttc ggcttgttgc cctctctcta acgctttccc acgggagaga ggacggtgcg
    11281 gctgtagtga ttaagggttt tgcaaggaaa acaaggaaaa caagttgtac aggctgtttt
    11341 ttatttttca agcgacctga aatcaacgct tgtaaatcca agtcgctttt caaactgcct
    11401 gcaaccgcaa aatctgctgc aacctacccc ctcccccgtg ggggagggct ggggagaggg
    11461 cattttccaa gttgcggcaa tctttcccaa atcccttagt cttctaaaat acaagcctta
    11521 cggttgttgc caaatgaaac ttcttccata cccaaaactt gcgtgtaaaa cgcaatggtt
    11581 cggtcaatgt cggcaacggt cagtaccaga tggtcgagtg cgctaatctt catttcccgc
    11641 tttctttttc aggcggcggc agtgccgtct gaaagccatt atgcccgttt tgccgctttc
    11701 atattgccaa tgaatttgtc aaacaaatag ccgacatctt gcggaccggg gctggcttcg
    11761 gggtggcctt ggaagcagaa cacaggtttg tcggtcagct cgatgccttg caaagtgttg
    11821 tcaaacaagg atttgtgggt aatgcgtgcg ttagcgggca gggtgtcggc atcaacggca
    11881 aaaccgtggt tttggctggt gatgacgact ttgccgctgt ccaaatcttg cacagggtgg
    11941 ttcgcaccgt ggtggctgaa gcgcattttc agggttttcg cgccgatggc gaggctgatg
    12001 agctggtgtc ccaagcaaat gccgaaaatc ggtttgccgc tttccatcag tttttgtacg
    12061 gctttgatgg cgtaggtgca aggctcgggg tcgccggggc cgttggatag gaatacgccg
    12121 tcagggttga gtgccaacac gtcttccgcg ctcgtttggg cggggacgac ggtcaggcgg
    12181 cagccgcgcg aggcgagcat acgcaggatg ttggttttca cgccgaaatc gtaggcgacg
    12241 acgtggtaag gctgttcgtc aggggtaacg aaacccttgc ccaatgccca ttcgccttcc
    12301 gtccattcgt aagtttccgt gcaggaaact tcttttgcca agtctttgcc gaccatactg
    12361 ccgaacgcgg cgatgagttc ttgcgctttt tcgattgtgg catccgcacc ggtcagaatc
    12421 gcgccgcctt gcgcgccttt ttcgcgcaac agcgtggtca ggcggcgggt atcgatgtcg
    12481 gcgatggcga cggttttgtt gcgtaccaga tagtcgtgca ggctttcgga ggcgcggaag
    12541 ttgctgtgca agagcggcag gtcgcggata atcaggccgg cggcataaac gctgcggctt
    12601 tcttcatctt cggcgttggt gccggtgttg ccgatgtgtg ggtaggtgag ggtaacgatt
    12661 tgtttgcagt aggacgggtc ggtcaggatt tcctgatagc cagtcatcga agtattgaac
    12721 acgacttcgc cggaagtcga accttcgtaa ccgattgatg tgccgtggaa tacgctgccg
    12781 tcagcgagga cgaggagggc gggggtgctc atgatgggaa tcctgttttt aataaaattc
    12841 ttgacaatgc cgtctgaagg gacttcggca gggggcatcc ggtcggaaaa atccggtcaa
    12901 aaaaaaacac gccgcgcaag atagacgcgt aacgtgcttt tcgggcaagt gcctataccc
    12961 tgaaagcagc atattttaag acgaaacata cccgatttca agcgcaaacg cggcaggcgg
    13021 gctgatatgt tcgcgcgaaa acggaaatcc gtggcgtaaa accgcccgct ccgcgcccac
    13081 tatgcccgtt acgcatttcc ccacgtccgt ccggcgaaac tatgggaata cccgaatcgt
    13141 caggaatacc tgaatcgtca ggaatacctg aatcgtcagg aatacctgaa tcgtcaggaa
    13201 tacccgaacc gtcattcccg cgcaggcggg aatccagacc tgtcggcacg gaa
//
LOCUS       DAWHXZ010000033        12570 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000033 DAWHXZ010000000
VERSION     DAWHXZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12570
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.033"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..166)
                     /locus_tag="WIE35_000615"
     CDS             complement(<1..166)
                     /locus_tag="WIE35_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ4153568.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITA"
     gene            complement(179..916)
                     /gene="hisA"
                     /locus_tag="WIE35_000616"
     CDS             complement(179..916)
                     /gene="hisA"
                     /locus_tag="WIE35_000616"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ4153569.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(949..1587)
                     /gene="hisH"
                     /locus_tag="WIE35_000617"
     CDS             complement(949..1587)
                     /gene="hisH"
                     /locus_tag="WIE35_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ4153570.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGYRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(1718..3220)
                     /gene="pta"
                     /locus_tag="WIE35_000618"
     CDS             complement(1718..3220)
                     /gene="pta"
                     /locus_tag="WIE35_000618"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ4153571.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMSQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDIVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(3405..4463)
                     /gene="fbpC"
                     /locus_tag="WIE35_000619"
     CDS             complement(3405..4463)
                     /gene="fbpC"
                     /locus_tag="WIE35_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ4153572.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(4484..6001)
                     /gene="fbpB"
                     /locus_tag="WIE35_000620"
     CDS             complement(4484..6001)
                     /gene="fbpB"
                     /locus_tag="WIE35_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ4153573.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(6069..7064)
                     /gene="fbpA"
                     /locus_tag="WIE35_000621"
     CDS             complement(6069..7064)
                     /gene="fbpA"
                     /locus_tag="WIE35_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ4153574.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALITYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(7315..7785)
                     /locus_tag="WIE35_000622"
     CDS             complement(7315..7785)
                     /locus_tag="WIE35_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ4153575.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGWEQELVLIW
                     RAEADIGNGGILQFVCNWSFPAAEKTCSVLKKIGAIHSAMLIHRAADALDKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(7842..9218)
                     /gene="argH"
                     /locus_tag="WIE35_000623"
     CDS             complement(7842..9218)
                     /gene="argH"
                     /locus_tag="WIE35_000623"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ4153576.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAVEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSKLPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMSA"
     gene            complement(9237..10106)
                     /gene="galU"
                     /locus_tag="WIE35_000624"
     CDS             complement(9237..10106)
                     /gene="galU"
                     /locus_tag="WIE35_000624"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ4153577.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(10134..10733)
                     /gene="rdgB"
                     /locus_tag="WIE35_000625"
     CDS             complement(10134..10733)
                     /gene="rdgB"
                     /locus_tag="WIE35_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ4153578.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAEYSGLPALADDSGICAAALNGAPGIHSARYAGDNPKSDTA
                     NNLKLAAELAGKTDKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHREQALADLIRKLAL"
     gene            complement(10726..10959)
                     /locus_tag="WIE35_000626"
     CDS             complement(10726..10959)
                     /locus_tag="WIE35_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153579.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(11067..11600)
                     /locus_tag="WIE35_000627"
     CDS             complement(11067..11600)
                     /locus_tag="WIE35_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ4153580.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(11702..12160)
                     /gene="nudB"
                     /locus_tag="WIE35_000628"
     CDS             complement(11702..12160)
                     /gene="nudB"
                     /locus_tag="WIE35_000628"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ4153581.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            12371..12447
                     /locus_tag="WIE35_000629"
     tRNA            12371..12447
                     /locus_tag="WIE35_000629"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12405..12407,aa:Pro,seq:ggg)
BASE COUNT         2499 a         3050 c         3947 g         3074 t
ORIGIN      
        1 aggcggtgat gtcgaggaag gtcaattcgt ccgcgccttc gccgttgtag cgtttggcgg
       61 cttcgacggg gtcgcccgcg tcgcgcaaac cgatgaagtt cacgcctttg acgacgcgcc
      121 cgtcttttac gtcgagacag gggatgatgc gttttgccag tgccataatc ggatgccttt
      181 agtcgaggga atctgccagt tgctgcgctt gggcaaaatc gatgctgccc tcgtaaatcg
      241 cgcggccggt aatcgcgcct gctacgccat gtttttcggc ggcacacagg gcgcggatgt
      301 cgtccaagcc ggtcagtccg ccggaggcga tgacgggaat gcggacggct tgggcgagtt
      361 tgaccgtcgc gtcgatgttc acgccgctca tcataccgtc gcgcccgatg tcggtgtaga
      421 tgatgctgtt gacgccgtcg tcttcaaagc gttttgccaa atcaattaca tgatgcccgg
      481 ttacggttgc ccagccgtcg atggcggcca taccgtcttt ggcatccagc ccgacaataa
      541 tcctgccggg gaaggctttg cacgcctcgc gcacgaagtc ggggtttttg accgccgccg
      601 tgccgataat cacgtcgttt aagcccaaat ccaaatattg tccgatggtt ttcaaatcgc
      661 gtatgccgcc gccgagctgt acggggatgt ctttggcgac agcggcaagg atgtctttga
      721 tggcgggcag gttttgcgga acgccggcaa acgcgccgtt caaatctacc agatgcaggc
      781 ggcgcgcgcc ttgtttgaac cagtgcagcg cggtttcggc gggcgaatcg gaaaagacgg
      841 tcgcctcttc catcagccct tgtttcaggc ggacgcagcg tccttctttc aaatcgatgg
      901 cgggtatcag cagcataatt tttctccttg tgcggggccg tgtccggctt accagtttaa
      961 aaagtttttc aacatcgtca gcccggcatc gtggcttttt tcggtgtgaa attgcgtggc
     1021 gaatacgttg tctttgccga cgatgcaggc aaacggggac gggtagtcgc tttcgcccaa
     1081 tatggtttcg ggattttcgg gggcgaaata gtagctgtgg acgaagtaaa aacgcgtgtc
     1141 ttggggaata tctttaaaca gcgggtggtt ttgggtttgg cgcacggtgt tccagcccat
     1201 atgcgggact ttcagacggt atccctgcgg gtcgcggagg tcgcgctcaa agcgtctgac
     1261 tttgccgccg aaccagccca agccgtcggt gtttccttct tcactgtggt cgaataaaag
     1321 ttgcgcgccg acgcagattc cgaaaaaggg tttgtttttt aaggcatctt tgactgcctc
     1381 gtccaggccg ccgcgcgtca gtgccgccat acagtcgggc atcgcgccct gcccgggaaa
     1441 aatgactttg tcagcgcggg acacgcgttc ggggtcgccg cttaaaaaga tttcggtatt
     1501 ttttccggca agctgccccg ccgtccggac ggatttcaat acggaatgca ggttgcccat
     1561 accgtaatcg ataatggcga tttgcatggc ttcctcctct ttttttgcaa tatggctgcg
     1621 attttaacaa acaaatgtgc cgtgctgata aaaatgccgt ctgaaaacgg gagtctgtct
     1681 tcagacggca tagggttcaa acccggaaag ccgtttgtca gccttccatt tgttttgcct
     1741 gaacggcagt cagggcgatg gtgaacacga tgtcttccac cagcgcgccg cgcgacaggt
     1801 cgttgaccgg tttgcgcagg ccttgcagca gcgggccgac gcttaagacg ttggcactgc
     1861 gttggacggc tttataggtg cagttgccgg tgttcaggtc ggggaagacc aaaacggttg
     1921 cctgtcctgc caccgggctg cccggagctt tggatttgcc cacgcccggc acggttgccg
     1981 catcatattg cagcgggccg tcgatggcga ggtcggggcg tttttcccgg gcaagtttgg
     2041 ttgcttcgat gacgatatcg acatcggggc cgctgccgga gttgacggtg gagtaggaaa
     2101 tcatcgccac tttcgggtcg atgccgaagg cttttgcgga atcggcagac tggatggcga
     2161 tgtcggcaag ctgttgcgcg gtcgggttcg gattaaccgc gcagtcgccg aagacgagga
     2221 cttggttggg cagcagcata aagaatacgc tggacacgag gcttgcgccc ggtgcggttt
     2281 taatcagttg caaagcgggg cggatggtgt tggcggtggt gtgaaccgca ccggatacca
     2341 aaccgtccac atcattttgc gacatcatca tcgtaccgag taccacggtg tcttgcagtt
     2401 gcttgcgcgc gtcttcgggt gtcaggcctt tggatttgcg cagttcgcac atcggctcga
     2461 cgtattgttc gaccaatgag gcgggatcga tgatttccaa agagtcgggc aggctgatgc
     2521 cgcgttcttt ggcaacggct tcgacttctt cgcgtttggc aagcaggacg cagcgggcaa
     2581 tgcctttttc gtggcagatg gcggcggctt ggacggtgcg gggttctgcg ccctcaggca
     2641 ggacgatgcg tttgtcggct tggcgggcga agtcgatcag gttgtagcgg aattgcgccg
     2701 gcgacaggcg ttttgcttcg cggcctgcca atacggatac gtctttcagc gcgtcgctcg
     2761 aaccgaagaa ggtcaggccg gttttttcgg ctgccgcttc ggcaacggag gctgccgcgc
     2821 cgtccacgac aaaaccttcc aatacgcccg gcgcggcggc gaagaactgt ttggcaaggt
     2881 tcagccgatt tgccagttcg tcggcatcgg cgttgtcgga acggacggcg aagacggctg
     2941 ccgcgtcaag ggacaatgcc agttcgacgt ttttgcctgc gaggtagatt ttgtcggcat
     3001 cgggcgcgat gccttcgatg acgaggttgg cggcatcgag tgcggcaact ttgccgacca
     3061 gtgcgtcgaa ccagtcgtcg cttttgcctt gcgcgagcag ggtttcggcg gttgcgtcaa
     3121 cggcttggaa aatttgtgcg tccagtgctt ttgcaaaggc ttgtgcggcg gcggaggcgt
     3181 ccagtccggc agatacgggt acgatgagta cttttgccat gatatatcct ttcgtatgct
     3241 gcggtgtgcg gcatatgtgg ttggaagggg cggcatatag gcagaaacgg ctgcctgcgt
     3301 gccgtgcgtg ccgtgtttgg cttgaggcgc gcaggttgaa tatagcaaac aaattctgtt
     3361 tccaacaaga taaatatccg caggcttgtg gatgctgccg cctttcagag ggtatttccg
     3421 gggaagaaca gggcgggacc gtccaaatgg aggacggcgg aaatgccgtc tgacagggtg
     3481 ggggcggaag ggaggttgag cgtgaggacg gtttgtccga cccggagact gatttcggta
     3541 tgccgcgctt tgggcgtggt tttgagaacc acggcgtgaa tggaggcggt gggtgcggaa
     3601 tgggggtgaa ggctgaactg ttccggacgg atgagcagtg tgccgcgtgt gcctgcgggt
     3661 gcgccgcttt gaacgggcag gcggcccaat ccgcaatcgg cggtgccgtc ggcgttgagc
     3721 gcggcgggga acacgatgcc ttcgccgata aacagggcgg catcaaggtc ggcaggttgt
     3781 cggtacaatt cgtgagggct tgcagtttgg aggatgcgcc cctgtttcat cacggcaatc
     3841 cggtcggcgt attgcagggc ttcttcgcgg tcgtggctga cgaaaacggc agatttgccg
     3901 ttggcgcgca gggcggcaat catgtcttcg cgaatctggc ggcgcaactg ttcgtccagc
     3961 gcgctgaagg gttcgtccaa caaaatcagt tcgggatcgg gtgcgagggc gcgggcgagg
     4021 gcgacgcgct gttgctgtcc gcccgaaagt tcgtgcggat agcgtccggc aagttcggaa
     4081 atgccggtca attccaacat agcttcgatg cgctgccgct cttgcgccgt cttgcctttg
     4141 ccgttgccca gcccgtaggc ggtgttgcgg taaacggtca ggtgggggaa cagcacacct
     4201 tcctgtacga cataacccaa acggcgttcg cggacgggga ggttggtatt tttcgagaag
     4261 atggttctgc cggaaagcga aatttcgcca aaatcgggtt gttcaaaacc ggcaaggcag
     4321 cgtaaaaggg tggttttgcc gcagccggac gcgccgacga taaagaggat ttcgcccggg
     4381 tcgaggctga gcgaaatgtc gtttaaaact ggggtgtttt gaaaactttt ggacaggtgt
     4441 ccgatgtgca gggcggcggt catggcggta cttcctcaag ctgttatttg aaggcgtatt
     4501 tcttcagcag gaatacgggg atgccggaaa ataataccag catcagcgcg taaggggtgg
     4561 cggcggcgta ttgtgcgtcc gatgtgtatt cccaaacggc ggtggagagt gtgtggacat
     4621 cgtcggtggt cagcagcagg gtggcggtca gctctttcat cagtttgagg aagacgagtg
     4681 cgaatgcggc ggtaatgccg ggcaggatgg acggcagtac caacgtcctg aaaataaaga
     4741 agtgtccgcg ccccaatgtt gcgccgacct gttccatccc ttttgggagt tgttccaagg
     4801 aagtcctcag ggtggtttgc gccatcggca ggtaaagcat gaaataggca aggatgacga
     4861 cgataaaggt ttggtaaacg gcaggggtgt agttgatgct gaaataaacc aaggataggg
     4921 cgataaccaa accggggacg gcgtgcagta aaaacggcag cctgtctatc caaacggtta
     4981 aaaaattgcg atagcgaacc gatgcccaaa caaggggcaa ggcacataat atagtcaaaa
     5041 tcgcacctaa agccgatacg cttaaggaac ggataaaggc atcaaatacg gatacgagcg
     5101 cgaatgtgcc ggaagtgccg accatcatcc aatgtatcaa tacgccaaag gggataataa
     5161 tgcccaaagt caacaagctg cttaaaaaaa caatcgcgcc aatctgaccg ggcagtttga
     5221 gggttttgac gggataagga cgggcaacgc ctttgccgct gtggtaaatc ttggctttgc
     5281 cgcgaaatat gctttctcca aatacgacga tgccgcacac cgccattaaa acagcggaaa
     5341 gcagggcggc ggtattgttg ttgtaggaca tttcgtattc ttggaaaatg gcggtggtaa
     5401 aagtggggta gttcaaaatg gataccgcgc caaattcgac cagcatatgc agggcaatca
     5461 gtaacacgct gctgccgatg gcgggtttga gctgggggag gatggcggaa aaaaaggttt
     5521 gcaggcggct tttgcccaag gacaggctga cttcttcgta agacaggctg atgcgtttga
     5581 gtgccgcctc gacgggcagg taggcgagcg ggaacgagga caggctcata atcatcactg
     5641 tcccccaaaa gccttcgaca cggaaggtca ggctgatcca ggtgaaacag ctgacaaatg
     5701 cggggatgca caaaggcagg gtgattgccg tctgaaaaaa ggttttgccg aagaagcggt
     5761 aacgttggaa caaaagggcg caggcaatgc ccaaaacaat ggaaatcagg gtaacgcccg
     5821 ccatcatcgt caaggtgttg gagagcaaat cccacatacg cgggcggaac aacagttcga
     5881 cggcgcggtt gatgccgacc tgccacgaac gcatagcgac atataaaaaa ggcagggtaa
     5941 gcggtagggc aatcagtagg atgaggccgg taagccaaat gggtattttt ttaggagaca
     6001 tagtgttttt catcggcaaa acgggcggac agtataaatg tccacccgtt tgacaatccg
     6061 aaaacggctt atttcatacc ggcttgctca agcagccggg tggcgtgttc tttttcggaa
     6121 acagtggtgg cggacacttg gggtgcttcc aacttggcga tgggttccaa attgaaggtg
     6181 gataccacgt gcggattcaa aggatattcg gcacggacgg cggtcagggc gcgctgtcct
     6241 tccttgctgg cgaggaaggc gacgaatttt ttcgcctcat ccttgttttg ggaggatttt
     6301 aacacggctg cgccggaata ggtaatgagt gcgccgggat ctctgtggcg gacgaaattc
     6361 aggcgggtgt ggacattttg tacgcctttt tcacgcgcga aagcgtgcca gtagtagttg
     6421 ttgatgaggg cggcatcgat ttcgccgttt tcaaccgctt gaagggcgac ggagttttta
     6481 gcgtaaggct tgccgtattc tttcagacct ttgagccatt tcaatgcggc cgcttcgcct
     6541 ttcagtttga cgatggcgac aacctgttcc aagaacgcgc cggaagtggg ggcgtaaccg
     6601 atgcggtttt tccatttcgg cgtggcgtaa ttcaggacgg atttttccaa atctttttca
     6661 gacagtttgc gggtgtcgta aacgacgacg cgcgaacgtc cgctcagtgc cacccagtct
     6721 tttttggcgg caaccggcac gcccttgccg cgtgtttcgt tgatggtgga ggcgggcagg
     6781 ggctctagga ggttggctgc ggaaagggtg gcgagtgccg ggatttgttc ggaatagaat
     6841 acgtcggcgg ggcttcggct gccttcttct ttgatttgac cggcaagctg gtcgcctttg
     6901 gcgctgttga gtttgacttt gatgccggta gcccgggtaa aggcatctgc aacggcttgt
     6961 gccgcttctt tgtgttggcc gttgtacacg gtaatgtctg ccagcgcggg ggttgcggcg
     7021 gtcagggctg cggcaagcag tgcgtatcgg atagatgttt tcatatcgat tttctcctaa
     7081 tgaatgagag tgtacacctt gttaagacat aacggcgtgt agtgtattcc ttcttcttta
     7141 taaatgcaaa taattatttt ttaaatttgt tattatccga tccggttatg gtttgttctg
     7201 acttgtattt tttccgtgca tcgcgcccgt aaggcggaag cggcgggcaa tgcgtggcgg
     7261 aatgtgggta aaggcggcat tttgatttgt cggaatgctt gagaacccct ctctttaaaa
     7321 cagcgtctga acagggttgc cggaatagta ttgccatccc ggcagataca gtttgtcggg
     7381 gtcttgccaa tattgttcat ccagactgtt cggcagtgag gcggttttgt tgtcgagatg
     7441 ttttgttata tcccacattt ctttcaggtt tttaccttcc gattggaggt ggcggatttc
     7501 tttgtccaat gcgtctgccg cccgatggat cagcattgcg ctgtgtatcg cgccgatttt
     7561 tttcagaacg gaacaagtct tttcggcggc ggggaaactc cagttgcaga caaattgcag
     7621 tatgccgccg ttgccgatat cggcttctgc ccgccaaatt aaaaccagct cctgttccca
     7681 cccgtccatg ctttcgagtt tgccgtcatg ctgttcaaaa agtttgtcca ccgcctgcca
     7741 catcatctgt tcgaagcggt cggcttgggt atccgtatcg gtcatagtgt tcttgtcttt
     7801 taaaaatgcc gtctgaacat ttcttcagac ggcatcgggg gttaagctga catttcccgc
     7861 cagcgtttga cttggaaacg gacttgttca ggcgcggtac cgcccaagtg gttgcgcgcg
     7921 tttaagctgc cttcgggtgt cagcacgccg taaacgtcgt cggcaatcaa atcgctgaaa
     7981 ccttgtaaga cttcgagcgg cagtttgctc aaatcgacac ccgcttggtc ggcgtggcgc
     8041 acggcttggg cgacgacttc gtgggcatcg cggaaaggca tgcctttttt taccaagtaa
     8101 tctgccaaat cggtggcggt agcgaagccc tgcatcacgg cggcgcgcat attgtcgggt
     8161 ttgacggtta cgccgcgcat catatcggcg taaatccgca acgtgtcgat aagcgtgtcg
     8221 gcggtgtcga acaagggttc tttgtcttcc tgattgtctt tgttgtacgc caagggctgc
     8281 gatttcatca gggtaatcaa gccgataagg tgtccgatga ctcggccgga tttgccgcgc
     8341 acgagttcgg gcacgtcggg atttttcttc tgcggcatga tggacgaacc tgtgcagaaa
     8401 cggtcggcga tgtcgataaa gccgaaacgc ggactcatcc acaaaatcaa ttcttcagac
     8461 aggcggctca ggtgaaccat aaccagcgag gcggcagccg tgaactcgac ggcaaaatcg
     8521 cggtcggata cggcatcgag cgagttttgg cagatttgct cgaaacccag taattcggcg
     8581 gtaatttcgc gccgaatcgg gtaggtcgta ccggcaaggg cggctgcgcc gagcggcata
     8641 cggttgacgc ggcggcggca gtccgccatc cgttcgttat cgcggccgag catttcgacg
     8701 taggcgagca tatggtgtcc gaagctgacg ggctgggcga cttgcaggtg ggtaaagcct
     8761 ggcatgacgg tttcggcgtt ttgttccgcc aaatccagca atgccgtctg aaggctttga
     8821 atcaggcttc ggatgacggt aatctggtcg cgcagccaaa ggcggatgtc ggtggcgact
     8881 tggtcgttgc ggctgcggcc ggtgtgcagg cgtttgcccg cgtcgccgat tttgtcggtc
     8941 aggcggcgtt cgatgttcat gtggacatct tccaaatcgg acgaccattc gattttgccg
     9001 ctgcggattt cttcgaggat ttccgccatg ccccgacgga tgtccgccaa atcgccttcg
     9061 tccaacacgc cggtttcttt cagcatttgc gcgtgggcaa gcgagccttg gatgtcccat
     9121 tcggcaaggc gtcggtcgaa accgatggag gcggtgtatt gtttgacgag ttcggaaacg
     9181 ggttcgttga aacgtccgga ccaggttttg tcgtgcataa ggattccttg atgtggttat
     9241 tcggtacggt atttttccaa aaggcggcgg aagggttcgc cggtttcggg gtgtttcagg
     9301 ccgtaggcga cggtggcttc cagatagccc agtttgctgc cgcagtcgta gcgcgtgcct
     9361 tcaaaggggt gcgctaggac aaattcgtga tcgagcagct tggcgatgcc gtctgtaagc
     9421 tggatttcgt tgcccgcgcc gcgcggcagt ccggtcagta agtcgaaaat gcgcggggtg
     9481 aggatgtagc gtccaacaac ggcaaggttg gagggcgcgt cttcgggctt gggtttttcg
     9541 acaatgccgg taatgcgttg gaactgtttg agctgttcgg tttcgacgat gccgtatgag
     9601 ccggtttgcg atgcttcaac ggtttctacg cccaaaatgc tgttgccgct gcgcccgtac
     9661 acttcgacca tttgtttgag cgcgcctttg ggggcatcaa tcaggtcgtc ggcaaggata
     9721 acggcaaagg gttcgtctcc gatggcggcg cgggcgcaca agacggcgtg tcccaagccc
     9781 agtgcttccg cctgacggat gtagaggcag gtaatgttcg gcggcaggat gttgcggacg
     9841 tgttccaaca atttgtcttt atggcgcatt tccaactcgg tttcgagttc gtatgccttg
     9901 tcgaaatggt cttcgatgct gcgtttgttg cgtccggtaa caaacaccat ttccgtgcag
     9961 ccggcttcca cggcttcttc tacggcgtat tggatcagcg gcttgtcgac gatgggcagc
    10021 atttctttcg ggcttgcctt ggtggcgggc aggaagcgtg ttcccatccc tgcgacgggg
    10081 aagacggctt tttttatcgg tttcattctt tttcctttgt attgttttga tgtttaaagg
    10141 gcgagtttgc ggattaaatc ggcgagtgcc tgctcgcggt ggctttcgcg gtttttgacc
    10201 tccgaatcca attcggcggc ggttttgccg tgtccgggca gataaaaata cgggtcgtaa
    10261 ccgaaaccgt tttcaccggc gggaacgccg ttccattgtc cgtgccatac gccctcggcg
    10321 ataattggac gcgggtcgtc tttatggcgg acaaaaacca atacgcagac gtagcagcag
    10381 cttttgtcgg tcttgccggc aagttcggcg gcaagtttca ggttgttggc ggtatcggat
    10441 ttgggattgt cgcccgcgta acgtgcggaa tggatgcccg gcgcgccgtt tagggcggcg
    10501 gcacagatgc cgctgtcgtc ggcaagcgcg ggcagcccgc tgtattcggc ggcatggcgt
    10561 gcttttgcca gcgcgttttc gacaaaggtg ggatagggtt cggggcattc gggtatgccg
    10621 aatgcggatt gcggcaatac ggtgatgctg taaggtttga ataagttgcc gaattcttcg
    10681 agcttgccgg cattgccgct tgccaaaacg attttttccg gtttttcaga catagcggtt
    10741 ttcctttgcg gcggattggg cggcgcgtag ggatttgtgc cgcaggtaga gggcgaggct
    10801 gccgatttgt ccgaacagtg cgccgaatgc gaaaagaaag gaggcgacgg cgaaggcttt
    10861 gctgccgacg gtcagcagat aagcacccaa gaggacgagc agcataaatg ccagtgtgaa
    10921 gagggcaaca gacaacagat agatttttcg gcggttcatg gcgttcggtc ggaaacggta
    10981 tgttcggatt atagccgatt gggacggtat tccctagagc ttggaaaaat gccgtctgaa
    11041 acggcgttca gacggcatgg cgggcgttat gcctgtttgt tccaacgttc gatggattct
    11101 ttgatgactt ttttcgcttc ttccgcatcg ccccacgatt cgactttggt cgtacctgct
    11161 tttttcaggt ctttgtaatg gttgaagtgg aactcgattt gtttgatcag ctgttgcggc
    11221 aaatcggaca aagttttgta ggcgttgccg ttgttgcggt cgtcggcagg aacgcagacg
    11281 attttgtcgt ccacttcgcc gtcgtcaacg aatttcatca cgccgataac gcgcgcttcc
    11341 aagaatacgc cggttgccaa aggttgttcg gtaacgagca gcacgtccaa ttcgtcgccg
    11401 tcttcgtcca aagtttgggg aatgaagccg tagttggtcg gtttggcgaa gatggcgggt
    11461 tcgacgcggt cgagttggaa tgcggccagt ttgcggttcc actcgatttt gtggttgctg
    11521 ccggcgggga tttcgttgac gacgttgatg atgccgccgt ccacatcgcc gggggtcagg
    11581 atttggttga agtctgccat ttggtttcct ttgtttggga aagtttgaag tttgaaagta
    11641 tagcacaaac gtccggctga aaatgcgccc gatgccgtct gaaagggtgt acgggcgcgt
    11701 gttaccgttt gcccaaaaac ctgcccagtt ccagaatcgc gcgcctgttg gacggggaaa
    11761 acactttttc cgccgcttct tccaagccga accagccgta ggagacgtgt tcttcgggtt
    11821 gcagggcgat gggcgtatca cgcgggattt cggcagagaa gaggtgttcg cggttttcaa
    11881 acacgccttt tggatagcgg tgccgccagt ggtggtagat ttcgtaaacc gtgctgtcgt
    11941 gccagtcttg aagctgcccg tccgccagca ggatgccggt ttcttcccaa acttcgcgcc
    12001 ttgccgtttg ggcgacggtt tcgcccggtt cgaggctgcc ggttaccgac tgccaaaatc
    12061 cttccggatg cgtgcgttcg atgagcagga tgccgccgtc cccgctataa aggacgacca
    12121 gtgcggagac ggggtatttg agcggttttg ccatcggcat ctttcggcgg gctgcggtaa
    12181 tgaaggggcg gattatagca aacgccgcac gttatggcgt ttatcctttt ccgtatcctt
    12241 ttttctgcac ggatgggacg cgccggtgtt tgccggtaaa ttttccgttg tgtcaaaaag
    12301 ataagggcgg ttgtgatttt aatgcttgcc aaagcgtcgg gcggaaacta taatccgaaa
    12361 cttatcgagt cggagtgtgg cgcagtctgg tagcgcactt gcatggggtg caaggggtcg
    12421 aaggttcgaa tcctttcact ccgaccaaaa attccgaaag ccgctttcaa aagcggcttt
    12481 tttgccgtcc gtatgattat gatgtagagt acgcggcgac agacattcaa atgccgtccg
    12541 aaaaccgttc agatggcatt tctttatctt
//
LOCUS       DAWHXZ010000034        12511 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000034 DAWHXZ010000000
VERSION     DAWHXZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12511
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.034"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..140)
                     /locus_tag="WIE35_000630"
     CDS             complement(<1..140)
                     /locus_tag="WIE35_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ4153582.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETA"
     gene            complement(195..617)
                     /locus_tag="WIE35_000631"
                     /pseudo
     CDS             complement(195..617)
                     /locus_tag="WIE35_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            723..1205
                     /locus_tag="WIE35_000632"
     CDS             723..1205
                     /locus_tag="WIE35_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ4153583.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGVMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(1257..2468)
                     /locus_tag="WIE35_000633"
     CDS             complement(1257..2468)
                     /locus_tag="WIE35_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ4153584.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            2691..5129
                     /locus_tag="WIE35_000634"
     CDS             2691..5129
                     /locus_tag="WIE35_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ4153585.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNSKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPSPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDSPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            5338..6648
                     /locus_tag="WIE35_000635"
     CDS             5338..6648
                     /locus_tag="WIE35_000635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ4153586.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDIFKEQAERRVSLGLILAKLVDENKLEPTEEQVKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            6744..7358
                     /gene="clpP"
                     /locus_tag="WIE35_000636"
     CDS             6744..7358
                     /gene="clpP"
                     /locus_tag="WIE35_000636"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ4153587.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(7415..8680)
                     /gene="ispG"
                     /locus_tag="WIE35_000637"
                     /gene_synonym="gcpE"
     CDS             complement(7415..8680)
                     /gene="ispG"
                     /locus_tag="WIE35_000637"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ4153588.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSVWRTAYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(8696..9457)
                     /gene="pilW"
                     /locus_tag="WIE35_000638"
     CDS             complement(8696..9457)
                     /gene="pilW"
                     /locus_tag="WIE35_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ4153589.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQKSFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(9460..10554)
                     /gene="rlmN"
                     /locus_tag="WIE35_000639"
     CDS             complement(9460..10554)
                     /gene="rlmN"
                     /locus_tag="WIE35_000639"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ4153590.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(10698..11123)
                     /gene="ndk"
                     /locus_tag="WIE35_000640"
     CDS             complement(10698..11123)
                     /gene="ndk"
                     /locus_tag="WIE35_000640"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ4153591.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPAEAAEGT
                     IRADFATSVSINAVHGSDTPENAALEIAYFFSQTEICPR"
     gene            complement(11182..12333)
                     /locus_tag="WIE35_000641"
     CDS             complement(11182..12333)
                     /locus_tag="WIE35_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ4153592.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKDSGG"
BASE COUNT         3021 a         3094 c         3381 g         3015 t
ORIGIN      
        1 gcggtttcat aacgtccgtg catggattgg gtgattgcgt aaaatgcgga aaacagcgcg
       61 gcgatggtaa aggagttggg cagcaggtag atactgtttt gacgcaggga gcgttgcggg
      121 gtgttggtcg gggtttccat agctgaagtg tctcacggac gtggagaatc gataggggat
      181 tataacccaa accattatcg aacaatacgc cctttaggat gaagccctcg gaggcggttt
      241 gtttttctga tggcggatgc tttgcggggc ggtttgactc tgcctgccac cgggtgtgtt
      301 gtttcggatt tttgccggat ggatattgtt gctgggcatt tctgggcttg ccggaaggta
      361 tcggggggag ggcatcggaa tatgcggcgc accgttcaaa ccggggcggc gtttccctat
      421 tttcaggggg ttgcaggttt caacaaattg aaagatattt tttgcatttc cgaccgtatg
      481 ctgattttga agcgggataa attataggac aaggtttttc gcccgccgca aaggttggcc
      541 gcaatgccgt ctgaaaacaa cggcttgccg ctaaggcggc ggtatgcgga tttgcgctgc
      601 cgaaatcggt ttgccatacc cggcggtgca atctgcgggg agggataagc cgataaggcg
      661 ggacttatcc tgttttttgt ttataatgca acagaatttg gacgaaccgt cgggaattta
      721 tcatgatgaa aaagatactg gccgtatcgg cactatgcct gatgactgcg gcggcacggg
      781 ctgccgatac ttacggctat ctcgccgttt ggcagaatcc gcagaatgcg gacgatgttt
      841 tgcaggttaa aactacaaaa gaagattcga cgaaaagcga agcgtttgcc gagttggaag
      901 ccttttgcaa aggtcaggac acgcttgcgg gcattgccga agacgagccg accggatgcc
      961 ggtcggtcgt gtcgctgaac aatacctgtg tcgcgctggc atacccgaaa gccttgggcg
     1021 tgatgcgcgt tgaaaacgcc gtcgtgatta cttctccgcg ttttacgagc gttcatcagg
     1081 tcgcactcaa ccagtgcatc aaaaaatacg gcgcacaggg acaatgcggc ttggaaacag
     1141 tgtattgcac gtcttcttct tattacggcg gaactgtgcg ctctttgatt caaaatctca
     1201 aataaaacgg aaaatgccgt ctgaaagatg ttcagacggc atttctatat cgacggtcag
     1261 gattctttcg gatcgggcag caggctgttc aacataatgg caagtacggc gcacaagccc
     1321 acgccggcaa agctgaagct gcccaatttg agcgtcatgc cgccgatgcc cgtggtcagt
     1381 accgagctga cgatgaccag gtttttcggc agcatcaaat cgactttggc atcaatcagc
     1441 gttttcacgc ccaaagaagc aatcgtgccg aacagcagca gcataatgcc gcccattact
     1501 ggcatcggaa tggaagccaa aaacgcattg aatttgccga aaaacgccat gcagacggca
     1561 aaaaccgccg cccaagtcat gatgacgggg ttgctgtttt tggtaatcat taccgcaccc
     1621 gttacttcgc cgtaggtcgt aaccggcggg ccgccgatca gacccgcaac gcatacgccc
     1681 aaaccgtcgc ctgcaagggt tttgtccaag cccgggtctt tcgtatagtc tttccccgtc
     1741 acattgccga ttgccatgat gccgccgatg tgttcgatgg cgggggcgac ggcaacgggc
     1801 agcataaaca gtgcagcctg ccagttgacc tgaggcgttt caaaatgggg aacggcgaac
     1861 cagggcgcgt gtgcaatgct tgccgtgtcc accagtccca tcagcagtgc caaaacataa
     1921 cccgaagcga caccgatcaa gataggaatc agcttcatca tcctgctgcc gaaaaccgat
     1981 acgatggcgg taacggcaaa ggtaaagccg gaaagaatca gcgaatcggt atagtcgatg
     2041 acctgtttgc cgtccgcctg acccattgcc atgctgcttg ccgccacggc aacagacaga
     2101 ccgatgacca tgatgacggg gccgatgacg accggaggca gcagtttgtg taccgctgcc
     2161 agtccgcgcc aacggatcag cgcggcaaac acaaaataca taaagccggc ggcaaacagt
     2221 ccgaacatgg tggaaggcag cccccattcg ccgacggagt agataatcgg tgcgataaag
     2281 gcaaacgaag aaccaagaaa aatcggcact ttgcgtttgg ttgtgatttg gaacagcagc
     2341 gttcccaagc ctgcgcccaa aagcgcaaga gccggattca gaccggtcag caggggaacc
     2401 agcaccattg cgccgaatgc cacaaataaa atctgtgcac cggaaacggc aagtttcagt
     2461 tggtttatac catcttcttt caatcggttc aaacggcacg gattatactg gtttgccgag
     2521 ggtctgcgta aaaatacata tggcgggcaa cagatatgcc gtctgaaagg caatgtctgt
     2581 ctgttgcagg atgaaagcag gagaaatgga gtataatccg acactttcca tattcaatcc
     2641 gcaagccgtc cggcttcctt atttcagacg gcattttccg gtaatacggc atgacagaaa
     2701 aatcccataa aaaaacagca aaaggcaggg cgggaagccc gtccccaacg tccgcacgca
     2761 acaagaaagc cgacaacggc gcacggggca ataaagtttc cgagcggctc aaggcggtca
     2821 aagagttgca gaaaaccgaa accaaaaagg cgcgtcccga acatgtcgtc aaccttatcg
     2881 gcgacgcact gtggctgatg ggtttggcgg caaccctgta tttggcgatt tccctgatca
     2941 gtttcgatat gggcgatccg tcttggtcgc acagttcgcc ggttgtggaa gatgtcgcca
     3001 attggggcgg actgttcggc gcgtatgttg ccgatgtcgg ctattatctt ttcggctggt
     3061 cgttctggtg gtggatagcg gctgcctgcg tcatgctgta taaaaatttt cgtctgcacg
     3121 caaaacagac ggaaaacgag gcatacaacc acaaaatcgc tgccgccgcg ctgtttgtcc
     3181 tgacggtctt cagccccgtc ttggagtatt ttgtgctggg cggaaaatat gccgactccc
     3241 tgcctgtcgg agcaggcggt atggtcggca tacgcgtcgg cgcagtgttt gcgtggctgc
     3301 tggggaaatc gggcagcctg ctgattatct tggttgttct gctgttgtcg ttgtccctgc
     3361 tggtgcagat ttcatggctg gaatttttga acggtgcggg cagggcggtt caaaaccgcc
     3421 tgagtgcctt atccggcaag gtcatggctt taggaaaacg ccggccgaat agcaaaacag
     3481 acggtgtcga tacccaaaat acacggcgca tggtaaaaga agccaagaat attacggcca
     3541 aacccgttgc cttgcccgaa ggcagcagca gcaaccgcaa atccgtcgcg gtttccgtcg
     3601 cgccgtcgcc caaaattcag gtttctctgt ttgaagatga cgaacctcgg caggcgggcg
     3661 aataccacaa gcctacattg aacctattgc ggattcctga cagcgaaccc gtcagcatca
     3721 atcccgccga attggagcgc actgccgaac tgatcgaatc caaactggca gaattcggca
     3781 tcggcgtaca agtcgtatcc gccacatccg gtcccgtcat cacgcgctac gaaatcgaac
     3841 ccgcgcaagg tgttaaaggc agccaaattg ttgccttgtc gaaagatttg gcacgctcta
     3901 tgtcgctgca atccgtgcgt atcgtcgaaa ccatcgcagg taaaaacacg atgggcatcg
     3961 agttgcccaa cgacaaacgc caagacgtga tgttgagcga aatcttgtcc tcgcccgtgt
     4021 ttgccgaagc caaatccaag ctgaccgtcg cgctgggcaa agacattgcc ggtacccccg
     4081 ttgtcggcga cttggcgaaa atgccgcacc ttttggtcgc cggtatgact ggttcgggca
     4141 agtccgtcgg cgtgaacggc atgattatgt ctatgctttt caaagctacg cccgacgaag
     4201 tccgctttat catgatagac ccgaaaatgc ttgagttgag catttacgac ggcattccgc
     4261 acctgctctg tcccgtcgtg accgatatgc gcgaagcggg gcaggcgttg aactggtgcg
     4321 tcgccgaaat ggaaaaacgc taccgcctgc tttcccacgc cggtgtacgc aatctggaag
     4381 gcttcaacca aaaagtcgaa gccgcaaaag cggcaggcaa gccgctgctc aatccgttca
     4441 gtctgaaccc cgacagcccc gagcctttgg aaaaactgcc gatgattgtg gtcgttatcg
     4501 acgaacttgc cgacctgatg atgaccgaac gcaaagccgt cgagcagcaa atcgcccgtc
     4561 tcgcccaaaa agcgcgcgcc gccggtatcc atatgattgt cgccacccaa cgtcccagtg
     4621 tcgatgtcgt tactggcctg attaaagcca acatcccgac gcgtatggcg tttaccgtgc
     4681 aaagcaaaat cgacagccgt accatcctcg accaaatggg cgcggacgaa ctgctcaaat
     4741 atggcgattc gctgttcctc cagcccggca gtgccgaacc gacccgcctg caaggcgcgt
     4801 ttgtttcaga cgacgaagta catcaagtcg tcaactacgt caaatcgcaa gccccagccg
     4861 actatattga aggtctgctc agcggcgagg ccgcgctgga aactgccaat atcgttaatc
     4921 cgaatgcaga cagcgacgaa ttgttcgatc aggcagtcgc ctatgttttg gaaagcaaaa
     4981 aaacctccat ttcgtctttg cagcggcagt tgcgtatcgg ctataaccgc gcggcaaacc
     5041 tgatggaggc actggaaaat gcgggtgtcg tttctcccac cgacctcaac ggcagccgta
     5101 aaattttggc gcacaaggac catttgtagc ccgtattgca aaatgccgtc tgaacggcgg
     5161 aattggcgtt tcagacggca tattatgttt caggcggaac attgtgatat acttgccagc
     5221 taaaatctcc cctttgcggc aatgcggttc aaatatccgt accgttgcgc tgtttgcttc
     5281 cccatgcagg gaagaaggtt taccatttta tcaacacaac aaatttaagg gcttatgatg
     5341 agcgtaactg ttgaaacttt agaaaatctg gaacgcaaag tagtgttgtc cctgccttgg
     5401 tccgaaatca acgcagaaac cgataaaaaa ctgaaacaaa cccagcgccg tgcaaaaatc
     5461 gacggtttcc gcccgggtaa agcaccttta aaaatgattg cccaaatgta cggtgcgagc
     5521 gcgcaaaacg acgtgatcaa cgagctggtg caacgccgct tccacgatgt tgccgttgcc
     5581 caagagttga aagtggcagg cttcccccgt tttgaaggcg ttgaagaaca agacgataaa
     5641 gaatctttca aagttgccgc catttttgaa gtgttccccg aagtcgttat cggcgatttg
     5701 tctgcacaag aagttgaaaa agtaaccgct tccgtcggtg atgccgaagt ggaccaaacc
     5761 gtagaaatcc tgcgcaaaca acgcacccgc ttcaaccatg tcgaacgcga agcccgaaac
     5821 ggcgaccgcg tcatcattga ctttgaaggc aaaatcgacg gcgaaccttt tgcaggcggc
     5881 gcatccaaaa actacgcctt cgtattgggc gcaggtcaaa tgctgcctga atttgaagcc
     5941 ggcgtagtcg gcatgaaggc tggcgaaagt aaagacgtta ccgtcaactt ccctgaagaa
     6001 taccacggca aagacgttgc cggtaaaact gccgtgttca ccattacgct gaacaacgtt
     6061 tccgaagcga ctctgcctga agtcgatgca gattttgcaa aagccttggg tattgcggat
     6121 ggcgacgttg ccaaaatgcg cgaagaagtg cagaaaaacg taagccgcga agtggaacgc
     6181 cgcgtaaacg aacaaaccaa agaatccgta atgaacgcgc tgctcaaagc cgtagagctg
     6241 aaagcacctg ttgctttggt caatgaagaa gccgcacgct tggcaaacga aatgaaacaa
     6301 aattttgtta accaaggtat ggctgatgct gccaacttgg atctgccttt ggatatattc
     6361 aaagaacaag ccgaacgccg cgtgtctttg ggtctgattt tggccaaact ggttgatgaa
     6421 aacaaactgg aaccgactga ggagcaagtc aaagccgttg tcgccaactt cgcagaaagc
     6481 tacgaagatc ctcaagaagt aatcgactgg tactacgcag agccttcccg cctgcaagcc
     6541 ccgacttctt tggcgataga aagcaacgtc gttgatttcg ttttgggtaa agccaaagta
     6601 aacgaaaaag ctttgtcttt tgacgaagtg atgggtgcgc aagcctgatt atcctgaaaa
     6661 tgccgtctta gggcagtttt tgaaagcacc gaagccgctt ttaaagcccg ctttggtgct
     6721 tttacatcat gaaaggagac gaaatgtctt ttgataacta tcttgtccct accgttatcg
     6781 agcagagcgg tcgcggtgag cgtgcattcg atatctattc ccggcttttg aaagagcgca
     6841 tcgtattctt ggtcggtccg gtaaccgacg agtccgccaa tctggtggtt gcccaactgt
     6901 tgtttttgga aagtgagaat ccggataagg atattttctt ctatattaac tcgccgggcg
     6961 gttcggtaac ggccggtatg tcgatttacg acaccatgaa ttttatcaag cccgatgtat
     7021 cgactttgtg cttggggcag gcagcaagta tgggcgcgtt cttattgtcg gcaggcgaaa
     7081 aaggcaaacg tttcgccctg cccaacagcc ggattatgat tcaccagcct ttaatcagcg
     7141 gcggcttggg cggtcaggca tccgatattg aaattcacgc acgcgaactg ttgaaaatca
     7201 aagaaaaact caaccgcctg atggcgaaac attgcggccg cgatttggca gatttggagc
     7261 gcgacaccga ccgtgataat ttcatgtccg ccgaagaggc taaagaatat ggtctgattg
     7321 atcaaatctt ggagaaccgt gcttctttac agttttaata agaaatctga gagaaacaaa
     7381 tgccgtttga aaacagttca ggacggcatt tgtcttatag ggactgtata gggatgattt
     7441 tgttctggtt gcgtttggag ccgttttcgc cataattggt tttaacatat tcctctacaa
     7501 tagctaggaa ttcagttgca atattatcac ctttcagtgt gactttgcgc tccccgtcca
     7561 cataaaccgg cgcgacgggt gtttctcccg taccgggcag gctgatgccg atgtcggcca
     7621 atttgctttc tccgggccca ttgacaacgc agcccatcac ggcaacattc agggattcca
     7681 cgcctggata agcagtacgc catacgctca ttttttgtcg caggtaattt tgaacatctt
     7741 gtgccagctc ttgaaatacg gtactggtgg tacgcccgca gccggggcag gcggtaacca
     7801 tcggcgtaaa cgaacgcaat cccatagtct gtaaaatctc ttgcccgacg acgacctcct
     7861 gagtacgcgg gctgccaggt tccggagtca gtgaaatgcg gatggtgtcg ccgattcctt
     7921 cttgaagcaa gacggataat gccgccgttg atgcgacaat gcctttgctg cccataccgg
     7981 cttcggtcaa acccaaatgc agcggatagg cgcaacggct gcccagttcg cggtaaacct
     8041 gaatcaaatc ctgaaccgcg ctgactttgc acgacaggat gattttgtct tcgggcagtc
     8101 ccaatagaac ggctttttcg gcagattcca aagcggagac aatcagtgct tccttcgtca
     8161 cttcttcggg cggtttcggc gcggaagaag cgaggttggc atccatcata cgtttggcga
     8221 ggctttgatc caaagaaccc cagtttacgc cgatgcggac ggctttatcg ttttcagcag
     8281 cagtccgaat cataaaggca aatttttcat cgccttttac gcctttgccg acattgccgg
     8341 gattgatgcg gtatttggac aatgctttgc cgcattctgg aaattccgcc aacaggcgtt
     8401 cgccgttgaa gtggaaatcg ccgataagcg gtgtggcata gcccatatcg tccaagcggc
     8461 ggcggatttc ggcaactttg gacgcggctt cggggctgtt gacggtaata cgcaccattt
     8521 cggatccggc atcgctcaat tccttaatct gcaatgcggt ggcttttgca tcggcagtgt
     8581 cggtgttggt catagattgg ataacgacgg gtgcttctga accgacggta atatgatcga
     8641 tgcggacttg atgcgtcttg cggcgttgga gtgtgttcat atggtttgaa tctgtttatt
     8701 gaccggtgag gacggtttgc aattcttccg agtaggggaa attcgcctgc aattgtgctt
     8761 catattcgta tgccgcctgt gcgttgccga gggctttggc aattttccag cctagcagca
     8821 aatcatcggc ctgaaggact tctaccctgc tttggtattt tttaaagtag taatcggcat
     8881 cgcccaactg cccggccagc attttggtgc gcgccagttc tttaaatgcg ggtgggaact
     8941 gcggctgggc ggcgagggaa cgtttcaaat aggcttccgc caatccgaat tgcccctgtt
     9001 ttgcgctgca tatgccttta ttcaggttgg caatataagg ggtcgggtag gtggggtcgg
     9061 ccagggcttt gtcgaaatat gccatagatt cggcagggcg gttgagcctg ccgcacagga
     9121 accaaccgta gttgttgttg atttcggcac tgtcgggttt gatggagagg gcttgccgga
     9181 aacttttctg cgccttgtcg ttaactttca ggtattggta gatttcggca cggaccagcc
     9241 aggcaagctc gtttttaggg tctgatttca gggcgtcttc aatacttgcc gtcgcctgac
     9301 ggtagtcctg accgcgcata tattccattg ccaactgggt tttgatattg gaaacctgat
     9361 tggctttttc tgcccgcgag gggcggtagg aagtgctgca cgcgcccaag gcaaggacaa
     9421 gtaataaaga gattcgtttg gatggcttaa aaggcataat tacccctgtt gtccgattaa
     9481 aatctgctgc catttttgtt ggcggcgcgt tttatcctga acctgccccg ccaactgtcc
     9541 gcaggcggca tcgatgtcgt cgccgcgcgt ttttcgtacg gtaacgacaa atcctgcctg
     9601 ctgcaaaata tcgcggaaca cacggatgtt ctcattgctg gagcgttcgt atccggagtt
     9661 tgggaaggga ttgaacggaa tcagattgaa cttgcaggga acatctgtga ccagttcgat
     9721 cagttcgcgc gcatgttgcg ccttatcgtt tattccgtcc aacatgacgt attcgaaagt
     9781 gatgaaatcc ctgggtgctt tgaccagata gcgttggcat gcggccatca attctttcaa
     9841 gggatatttt ttgttcaacg gtacgatttg gttgcggact tcgtcattgg aagcgtggag
     9901 ggaaaccgcc aaagccaccg gcatgacatc gcgcaacctg tccatttggg gaaccatacc
     9961 cgaagtggaa acggttacgc ggcggcggct caaaccgtag ccgtggtcgt ccagcatgat
    10021 gcttaaggcg gtaacgacat tgtcgaagtt cgccatcggc tcgcccatgc ccatcatgac
    10081 gacgttggaa atcacgcgct cgtttttcgg tgtaacgccc atcgctttgt ttgcccacca
    10141 caattgcccg atgatttcgg cagcagtcaa attgcggttg aagccctgcc ggccggtcga
    10201 acaaaatgta cattccaaag cgcagccgac ttgtgaggaa atgcagagcg tgccgcgatc
    10261 cgattcgggg atgaagacgg tttccacccc gttgcccgta ccgacatcca aaagccattt
    10321 tcgagtgccg tctgaagatt tttgagacat catcagcttg ggaatttcga tgcctgcctg
    10381 ttcgttcagt ttatggcgca acgattttgc caaatcggtc atttcgtcaa aattttgcgc
    10441 gccggattgg tgcatccaac gcataacctg tttggcacgg aaaggttttt cgcccatatc
    10501 ggcaaaatgt cgggtcagcc cttggaggtc gtagttgagc agattggttt tcatgtattt
    10561 gtgtttttct taaattaagg cttcaaataa aaaattgcag ggcaggcaag tttgatttga
    10621 agccttttgc aacagagttt tcagacggca tggatatatt ttatgccgtc tgaagaggag
    10681 ttgtgcggtg tattgtatca acgggggcag atttcggttt ggctgaaaaa gtaggcaatt
    10741 tccaaagcgg cattttccgg agtgtccgag ccgtgtacgg cattgatgct gaccgaagtg
    10801 gcaaagtccg cgcgtatcgt gccttcggcg gcttcggcgg gattggtcgc ccccatcagt
    10861 tcgcggtttt tcagaacggc gttttcgcct tccagtactt gaatcataac cggaccgccg
    10921 gtcataaatt caaccaatcc ggcgtagaag gggcggtctt tatgaaccgc ataaaattct
    10981 tgcgcctctt tgagggtaag ctgcttcatt ttggcggcaa cgattttcag accgttctcc
    11041 tcaaagcggc tgtatatttt gccgatgacg tttttgccga cggcatcggg tttgatgatg
    11101 gagatggtac gttcaatcgc catgctatat ccttattttt acttaagaag aatcaaatcg
    11161 gtattctatc aaaaaaataa cttatccgcc tgaatccttg ggagataggg tcaggtgcgg
    11221 ctgttccaaa taaacttcgg actgcatttc gaccatccgg ctggcggtgc gggtaaattc
    11281 ttcggcaaaa tcgccttccg tatagatatg gttgacatct accgcgccgg tggcacacag
    11341 tttgacccgg aaatcgtaga gtacgtcaat cagccaagtc agccgccgcg cctccgcctt
    11401 ttcttgcggt gagagttgtt ccaaacctga aataaaaacc atttcataat gttcggccaa
    11461 atacagatag tcggactgtg agcgggggcc gaagcacagt gcgcggaaat caaaccatat
    11521 ggcacggccg gactcggctt tgtggggaat ctcccgaccg tggatggtgc tgatgccggg
    11581 gttcaaatcg gtaatgcctg tcatttcttt gaacagtttt gccagttttg cctcattttc
    11641 ttcattggca ggcgtaaaga aaatctcggc ggggcggagg gtacgcagtc ggtagtcttc
    11701 accgccgtca acgtttaaga cggtcaggct ggactcgatg agcgcgattg tgggaagaaa
    11761 actgctccgg ttttgacctt gcgggtagag ttcggaaggc gcgtagtttg aagtcgccac
    11821 caaaacaacg ccctcgttaa gcaggttttc cagcagacgg cctaaaatca ttgcatccgc
    11881 aatatcgctg acatgaaatt cgtcaaaaca taatacgcgg gtttctttgg caatctcggc
    11941 ggcaacggat ttcaacgggt tgctttcgct tttcagggtt ttcagcctct ggtggatttc
    12001 tgccataaag gcatgaaagt ggacgcggcg tttgcggcgg taagggaggc agccgaaaaa
    12061 agcgtccatc agaaagcttt tgccgcgtcc gaccccgcca tagaaataaa gccctttggg
    12121 gacttgcggg gaacgcaaac tcctgcctaa aaaacggttt cttttgcgtt tgaacatcat
    12181 caattcggtc caaagccgat cgaggtgttc gatggcggct gcctgcgcgt cgtcgcggat
    12241 gaagttgggc agttgtgagg cagcctgata ccaggtcagc gggctgtggt tttcaaacgg
    12301 cggggcttta aaaagttggt ctctattact catatttaac cttgttatac ttcttgcggc
    12361 ggagaaagaa aatgcttacc gtcggtatta taaatgattt ccttcaaact tggcattttc
    12421 cgtggaaaag aataatgacg cgcacaaggg ggtataagca aaggcggcgt ttcaatatgg
    12481 tgcagatatg agctcaaatg ccttttatag t
//
LOCUS       DAWHXZ010000035        12471 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000035 DAWHXZ010000000
VERSION     DAWHXZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12471
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.035"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..206
                     /locus_tag="WIE35_000642"
     CDS             <1..206
                     /locus_tag="WIE35_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEZ4153593.1"
                     /translation="PRGAGGEIQLTDGIARLLDHEFVLAHAFEGKRYDCGSKLGYLEA
                     TVAYGLKHPELKDDFKELLKQFV"
     gene            289..1305
                     /gene="galE"
                     /locus_tag="WIE35_000643"
     CDS             289..1305
                     /gene="galE"
                     /locus_tag="WIE35_000643"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ4153594.1"
                     /translation="MNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPRL
                     KTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPIKYYDNNVS
                     GSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPPGDTTSPYGASKSMVERIL
                     TDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAVF
                     GDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRAF
                     EAASGLTIPFEIKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVSNHPN
                     GYDD"
     gene            1401..2462
                     /gene="rffG"
                     /locus_tag="WIE35_000644"
     CDS             1401..2462
                     /gene="rffG"
                     /locus_tag="WIE35_000644"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ4153595.1"
                     /translation="MKKILVTGGAGFIGSAVVRHIINDTQDAVVNVDKLTYAGNLESL
                     ESLANHPRYAFEQVDICDRAALDRVFAEHQPDAVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWHDMPSEKKAAFRFHHISTDEVYGDLHGTDDLFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYG
                     DGMQIRDWLFVEDHARALYQVVTEGEIGETYNIGGHNEKANIEVVRTICALLEELVPN
                     KPQGVARYEDLITYVKDRPGHDVRYAIDAAKIGRELGWQPQETFESGIRKTVEWYLNN
                     QTWWSRVLDGSYNRERLGS"
     gene            2675..3550
                     /gene="rfbA"
                     /locus_tag="WIE35_000645"
     CDS             2675..3550
                     /gene="rfbA"
                     /locus_tag="WIE35_000645"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ4153596.1"
                     /translation="MKGIILAGGSGIRLYPITRGVSKQLLPVYNKPMIYYPLSVLMLA
                     GIRDVLVITTPEDNDAFRRLLGDGSDFGIQISYAIQPSPDGLAQAFLIGEEFIGDDSV
                     CLVLGDNIFYGQHFSQSLKEAAASVETKGATVFGYQVKDPERFGVIEFDENFKALSIE
                     EKPKQPKSDWAVTGLYFYDNRVVEFAKQVKPSERGELEITTLNQMYLDDGSLNVQLLG
                     RGFAWLDTGTHASLLEAASFVETIENIQNLQVACLEEIAWRNGWLSSEEIRNLAKPMC
                     KNEYGRYLLRLVGKD"
     gene            3605..4144
                     /gene="rfbC"
                     /locus_tag="WIE35_000646"
     CDS             3605..4144
                     /gene="rfbC"
                     /locus_tag="WIE35_000646"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ4153597.1"
                     /translation="MNIIDTRIPDVKLLEPKVFGDERGFFMETFRDEWFRQNVAERTF
                     VQENHSKSTQGVLRGLHYQTENTQGKLVRVVSGEVFDVAVDMRRDSPTFGHWVGEVLS
                     ADNKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPNAEHSLIWNDATVGIDWPLLGE
                     PSLSAKDLAGKMLAEAATF"
     gene            4306..6354
                     /locus_tag="WIE35_000647"
     CDS             4306..6354
                     /locus_tag="WIE35_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ4153598.1"
                     /translation="MIPAYIPLRSFGIRKIPNITQFLPEYSLNKTVFNAQTIIGWGLR
                     PTTHQARAFAQSHGLPFVALEDGFLRSLGLGVAGYPPFALVYDDIGIYYDTTRPSRLE
                     QLILAANMPSEMLNQAQQAIDLILTHRLSKYNHALDFSDGLVQDSKNIVLVIDQTFGD
                     MAIQYGGADEATFRQMFQTALAENPDAEIWVKTHPDVLSGKKQGYLTDLAQQPRVRLL
                     AEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVTFGLPWYAGWGVSDDRHPEINRL
                     VQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVIDYLATVKRKNDKLRGELY
                     CVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVKLSDDARILAWGNGKEAIVRFAE
                     QHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMGIYFNAEAPSRLEHILQNQNFDDQ
                     DFQTALKLQKMLTENHISKYNVGNSDFTAPSTDKTVILVPGQVEDDASIRYGSPQIYH
                     NLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISPEDAARYADQTAEQADILTCLQY
                     ADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLTQDLLPIPRRSRRLELWQLIAGT
                     LIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNNGLHRGCFAKKLGKIKQLYRSFK
                     "
     gene            6491..7750
                     /locus_tag="WIE35_000648"
     CDS             6491..7750
                     /locus_tag="WIE35_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide modification protein
                     LipB"
                     /protein_id="HEZ4153599.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            8117..9523
                     /locus_tag="WIE35_000649"
     CDS             8117..9523
                     /locus_tag="WIE35_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ4153600.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYTEAAFVLG
                     DHLLQNGHPEQALPWLEAAAAQRHPKALFTLLQQREHNGTPTGQLLNEYAWLSEQGHL
                     EAQLILMRYHTQRNDPQSLYWAELAAAQYAAPAYYHLARHHQRQGDVATAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEQAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKDHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAETQAHAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            9741..10955
                     /gene="gltS"
                     /locus_tag="WIE35_000650"
     CDS             9741..10955
                     /gene="gltS"
                     /locus_tag="WIE35_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ4153601.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            11054..12070
                     /locus_tag="WIE35_000651"
     CDS             11054..12070
                     /locus_tag="WIE35_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ4153602.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            12081..12278
                     /locus_tag="WIE35_000652"
     CDS             12081..12278
                     /locus_tag="WIE35_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153603.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGVEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            12296..12445
                     /locus_tag="WIE35_000653"
     CDS             12296..12445
                     /locus_tag="WIE35_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153604.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFLCVSYADVPTHRTIQKAS
                     PYVPS"
BASE COUNT         3181 a         3303 c         2987 g         3000 t
ORIGIN      
        1 tacctcgtgg tgcaggtggt gaaatccagc taactgatgg tattgcccgc ctacttgacc
       61 atgaatttgt cttggcgcat gcgtttgaag gtaagcgtta tgattgtggc agtaagttgg
      121 gctatctgga agccacggta gcttatggct tgaagcatcc cgaattaaaa gatgatttca
      181 aagaattatt gaagcaattt gtataatttt aaggccgtct gaaaaagaaa aggcatcatg
      241 ttttttacac tttcaggcgg ccttttaaac ataaagaagg aaaacgtgat gaacattctc
      301 gttaccggcg gcaccggttt tatcggctcg cacaccgttg tttctttgct gaaaagcggt
      361 catcaagtcg tgattttgga taacctatgc aattccagca tcaatatcct gccacgcttg
      421 aaaacgatta ccggccaaga gattccgttt tatcaaggcg atatccgcga tcgtgagatt
      481 ttgcgccgta tttttgcgga aaaccgcatt gattcggtga ttcattttgc cggcttgaaa
      541 gcggtgggtg aaagtgtggc cgagccgata aaatattatg ataataatgt ttccggcagc
      601 ttggtgttgg cggaagaaat ggcgcgtgcg ggcgtgttta gcattgtgtt cagttcttcg
      661 gcgacggttt atggcgatcc gggcaaagtg ccttataccg aggatatgcc accgggcgac
      721 accaccagcc cttacggcgc atcgaaatcg atggttgagc gcattctcac tgacattcaa
      781 aaagccgatc cgcgctggag catgattttg ttgcgttatt tcaatccgat tggcgcgcat
      841 gaaagcggtt tgattggcga gcagccaaac ggtatcccaa ataatttgtt gccttatatc
      901 tgccaagtgg cggcaggcaa actgccgcaa ttagcggtat tcggtgatga ctaccctacc
      961 cctgatggca cgggtatgcg cgactatatt catgtgatgg atttggcaga aggccatgtc
     1021 gcggctatgc aggcgaaaag caatgtagca ggcacgcatt tgttgaactt aggttccggc
     1081 cgcgcttctt cggtattgga aatcatccgc gcatttgaag cggcttcggg tttaaccatt
     1141 ccatttgaaa tcaaaccgcg ccgcgccggt gatttggcgt gcttctatgc cgacccttct
     1201 tatacgaagg cgcaaatcgg ctggcaaacg cagcgtgatt taacccaaat gatggaagat
     1261 tcatggcgct gggtaagtaa tcatccgaat ggttatgatg attaagtcat gaaattgaga
     1321 ggccgtctga agtttcagac ggcctttgtt tgcgatttat agtaaaattt accttatcat
     1381 tttggcgatt aatattcatc atgaaaaaaa tccttgtcac cggcggtgcc ggttttatcg
     1441 gttcggcggt tgtacgccat atcatcaacg atacgcaaga tgcggtggtg aatgtggata
     1501 agctcacgta tgcgggcaat ttggaatcgc tggaatcttt ggccaatcat ccgcgctatg
     1561 cgtttgagca agtcgatatt tgcgaccgcg cggcactgga tcgtgtgttt gccgaacacc
     1621 agccggatgc ggtgatgcac ttggcagcag aaagccatgt tgaccgcagt attgatggcc
     1681 cagcggcatt tattgaaacc aatattgttg gtacttacac gcttttggaa gcggcgcgcg
     1741 cgtattggca tgatatgccg tctgaaaaaa aagcggcttt ccgtttccat catatttcga
     1801 ctgatgaagt gtatggcgat ttgcacggca cggatgattt gtttaccgaa accacgcctt
     1861 acgccccttc cagtccttat tcggcttcca aagcctccag cgaccatttg gtgcgcgcat
     1921 ggctgcgtac ttacggcctg ccgaccattg tgaccaattg ttctaataac tacggccctt
     1981 atcattttcc tgaaaaactg attccactga tgattctgaa tgcgcttgat ggcaaaccgc
     2041 tgccggtgta tggcgacggt atgcagattc gtgattggct gtttgttgaa gatcatgcgc
     2101 gtgcgctgta tcaagtggtc acagaaggcg aaatcggcga aacttacaac attggtggcc
     2161 acaatgaaaa agccaatatt gaagtggtgc gcaccatttg cgccctgctg gaagaattgg
     2221 tgccgaacaa accgcaaggc gtggcgcgtt atgaagattt gattacctat gtcaaagacc
     2281 gccccggcca tgatgtgcgc tatgcgattg atgccgcaaa aatcggccgt gagttgggtt
     2341 ggcagccgca agaaactttt gaaagcggca tccgcaaaac agtggaatgg tatttaaaca
     2401 atcaaacttg gtggtcgcgc gtgttagacg gcagctacaa tcgtgaacgc ttaggcagtt
     2461 aaatagagac ctttgcaaac tccccatctt tggcacattt cttcgttatg cgctgctcga
     2521 aagcttgcct atctttgtga tatgtctgcg ctttctgcgc tgctataact tcgaactgta
     2581 ctcaaatctg tggctttgca aaggcctcaa ataataaaaa tacgttcaga cggcatgaag
     2641 gccgcctgaa aaactacata aggaaagtaa actgatgaaa ggcattattc tcgcaggcgg
     2701 cagcggtatc cgcctttacc cgattacccg cggcgtatca aagcaattac taccggtgta
     2761 caacaaaccg atgatttatt accccttgtc ggtgttgatg ttggccggaa tccgcgatgt
     2821 gctggtgatt accacccccg aagacaacga tgctttccgc cgcttactgg gtgatggcag
     2881 cgatttcggt attcaaattt cctacgccat ccaacccagc cccgatggtt tggcgcaagc
     2941 ctttttaatt ggtgaagaat ttatcggcga tgattcggtg tgtttggtgt tgggcgacaa
     3001 tattttctat ggccagcatt tcagccaatc cctgaaagaa gccgccgctt ccgtggaaac
     3061 caaaggtgct accgtattcg gctatcaagt gaaagatccc gagcgtttcg gtgtgattga
     3121 gtttgatgag aatttcaaag ccctgtcaat cgaagaaaaa ccgaaacaac ctaaatccga
     3181 ttgggcggta accggcctct atttctacga taatcgcgta gtcgagtttg ccaaacaagt
     3241 gaagccgtct gaacgcggcg aattggaaat taccaccttg aatcaaatgt atttggacga
     3301 tggttcgctc aatgtccaat tgctcggtcg cggtttcgca tggcttgata ccggcacaca
     3361 cgccagcctg ttggaagcag cctcgtttgt cgaaaccatt gaaaacattc aaaacctaca
     3421 agtcgcctgt ttggaagaaa tcgcatggcg taatggctgg ttgagcagcg aagaaatcag
     3481 aaacttggca aagccaatgt gtaaaaatga atatggccgc tatttattgc gcttggttgg
     3541 caaagattaa gaggttgctt ttaccagttt tcaggccgtc tgaaacgctt tttaggaaaa
     3601 catgatgaat atcatcgata cccgcattcc cgatgtcaaa ttgctcgaac ccaaagtctt
     3661 cggtgacgag cgtggttttt ttatggaaac cttccgtgat gaatggttcc gccaaaatgt
     3721 agccgagcgc actttcgtgc aggaaaacca cagcaaatcc acccaaggcg tattacgcgg
     3781 tttacattat caaaccgaaa atacacaagg caaactggtg cgcgtggtat caggcgaagt
     3841 ctttgatgtt gccgtggata tgcgtcgtga tagtcctact ttcggccact gggtcggtga
     3901 ggttttatcg gcagacaaca aacgccaatt atgggtgccg gaagggtttg cccatggatt
     3961 ttatgtgttg agcgatgaag cggaattcgt ctacaaatgc accgactatt acaacccgaa
     4021 tgcggaacat tccctaattt ggaatgatgc cacagtggga atcgactggc ctttattggg
     4081 agaaccaagc ctatcggcca aggatttggc aggcaagatg cttgctgaag cggccacatt
     4141 ttaattttca cttttaggtc atcgtgggta atttgtagca agatgaccta atttttacca
     4201 gctagcctac taactaaatt tctactatat ttaaatagtc agaaaattta cacaaaatct
     4261 gttaataaga tattatattg tcagtaatat tttttcctat ttattgtgat tcctgcctac
     4321 attcctcttc gctctttcgg tatacgtaaa attccgaata ttacgcaatt tctaccggaa
     4381 tattcactta acaaaaccgt attcaacgcc caaaccatca ttggctgggg cttacgcccg
     4441 accacccacc aagcacgtgc ttttgcacaa tcacatggct tgccgtttgt agccttggaa
     4501 gacggttttt tacgctcact gggcttaggc gtggccggtt atccgccctt cgcactggtt
     4561 tacgacgata tcggcatcta ttacgacaca acccgcccat cgcgtttgga gcagttgatt
     4621 ttagccgcaa acatgccgtc tgaaatgctt aaccaagcgc agcaggccat tgatttgatt
     4681 ttgacgcacc gtttgtctaa atataaccat gctctggatt tttcagacgg cctcgtacaa
     4741 gacagcaaaa acattgtttt ggtaattgat caaactttcg gcgatatggc gattcaatat
     4801 ggcggcgcag atgaagccac attccgccaa atgtttcaga cggccttagc cgaaaatcct
     4861 gatgcggaaa tctgggtcaa aacccatccc gatgtattga gcggcaagaa acagggttac
     4921 cttactgatt tggcacagca gccgcgtgtg cgtttattgg ctgaagacat taatccgatt
     4981 tcgctgctgc aagcggtgga caaagtgtat tgcgtgacct cgcaaatggg ttttgaagcc
     5041 ttgttgtgcg gcaagccttt ggtaacgttt ggtttgccgt ggtatgccgg ctggggtgta
     5101 agcgacgacc gccatcctga aatcaaccgc cttgttcaaa cccaacgccg cgccacccgc
     5161 aacttgctgc agctcttcgc cgcagcctat ctgcaataca gccgctacct caaccccaat
     5221 accggcgaag caggcagcct ctttgatgtc atcgactatc tggcgacggt caaacgtaaa
     5281 aacgacaaat tgcgtggcga gttatattgc gtcggtatgt ctttgtggaa acgcgcggtt
     5341 gccaaaccgt tctttaacgt accctcttgc cgtctgaaat ttatctcttc cacccaaaaa
     5401 ctggcaaggg tcaaactgtc cgacgatgca cgcatcctgg cttggggcaa cggcaaagag
     5461 gccatcgtcc gctttgccga acaacaccac atccccctgc tgcgcatgga agacggcttt
     5521 atccgctcgg tcggactcgg ctccaactta gtgccgccgc tgtcgctcgt taccgacgat
     5581 atgggtattt acttcaatgc cgaagcccca tcccgccttg aacacatcct gcaaaaccaa
     5641 aacttcgacg atcaagactt tcagacggcc ttgaagttgc aaaaaatgct gaccgaaaac
     5701 catatcagca aatacaacgt cggcaactca gactttaccg ccccatcaac cgacaaaacc
     5761 gtgatcctcg ttcccggtca ggttgaggat gatgcgtcta tccgctatgg ttcgccccaa
     5821 atctatcaca atctggattt gctccgtacc gtacgcgaac gaaaccccaa tgcctatatc
     5881 atctacaaac cgcatcccga tgtagtcagc ggtaaccgca tcggccatat ttcccctgaa
     5941 gatgctgcac gatatgccga ccaaaccgcc gaacaagccg acatcctgac ctgtctccaa
     6001 tacgcagacg aaatacatac catgacttcg ctgaccggtt ttgaagcctt gttgcgcggc
     6061 aaaaaagtca gctgctacgg cctgcctttt tacgcaggct gggggcttac ccaagatctg
     6121 ctccccatcc cgcgccgtag ccgcagactt gagctttggc agctgattgc cggcacgctc
     6181 atccactatc ccgactacat ccaccccgaa acccatcagg ccataaatgc ggaaaccgca
     6241 gcccaaatcc tgatacgaca aaaaaatatg caaaaaaaca acaacggatt acatcgcggg
     6301 tgctttgcca aaaaattagg taaaatcaaa caactatatc gatctttcaa ataaatacca
     6361 tcaaagttaa cgatgcgtca taaacttgcc tctattgcgg catcattgcc tttgcatcgt
     6421 taattctctt ggcgtatgct tgaaagttca acctaaaact attacataaa aaacaaaacc
     6481 acattgcaac atgaaacaga ccgtcctcaa aaataacctg caaaacctgc ttgaaagcgc
     6541 agaaaatatc ctgctgcttc aaggccctgt cggcgatttt tttctgcgcc ttgccgactg
     6601 gctgactgca aacggcaaaa ccgtacataa attcaacttt aatgcaggcg acgactattt
     6661 ttatccgccc actcaagcgc ataccgttgt ttttaacgac aactacgatg cctttcctga
     6721 gtttttgcaa gaatacatca ctcaacatca catccaggcc gttgtctgct ttggcgacac
     6781 acgcccttat cacgtcattg caaaacgcat tgcaaacgaa aaccaagcca gtttctgggc
     6841 gtttgaagaa ggctatttcc gcccctacta catcacctta gaaaaagacg gcgtcaacgc
     6901 attttccccg ttgccgcgcc gtgccgactt ttttcttgaa caattcccta agcttgccca
     6961 gcaagaatat aaagcgccaa cgccggtaca cggcggtttt acgcccatgg caaaaaacgc
     7021 tatccgttac tatatcgagt tgttccgcaa tccacgcaaa taccccgact acatccacca
     7081 ccgcgcaccc aatgccggcc attacctcaa accgtggtcg ctctccatcc tcaagcgttt
     7141 gaactactat attgaagaca tccaaatcgc caaacgtgtg gaagcaggca aatacggcaa
     7201 gttttttatt gttcccttac aggtattcaa cgacagccaa gtccgtatcc attgcgactt
     7261 tcccagcgtc cgcagcttcc tgctccatgt tttgagttca tttgccgagc acgcgcctgc
     7321 cgataccaac atcatcatca agcatcatcc gatggaccgc ggttttatcg actactggcg
     7381 cgacattaaa cgctttatca aagaacaccc cgaactcaaa ggccgcgtga tttatgtcca
     7441 tgatgtcccc ctgcccgttt tcctgcgcca cggtctcggc atggtcacca tcaacagcac
     7501 cagcggcctg tccggactga ttcacaatat gccagttaag gttctcggcc gtgcctatta
     7561 tgatattccc ggcattactg accaaaatac cttggcagaa ttttggaatc atccgacacc
     7621 gcctgacaaa gagctgttcc atgcctaccg aatgtaccac ctcaacgtga cccaaattaa
     7681 cggcaacttc tacagtcagg tgtttttccc caacaaaaaa acctccaact cttccacacc
     7741 agtaatctga cttagcgaag gaagttcagg ccgtctgaaa acatttcaga cggcctgaaa
     7801 caatcaatac cttagctact gccatgtaaa taaaacacaa aaatctgcat ttatcattaa
     7861 caataaatta caaaaacagt ataatgaccg agctgccatg agcgcatacc gactcaacct
     7921 gagcccttta taacacacaa aatatggatg tatccccagg caaaacaata taacaagcca
     7981 aacatcctaa agataagccg gcaaggaaat acactctgca aaacgatgcc gagcaatatt
     8041 tttacaaagc cctcaaccgg tatcgccgcc catatgccgc agcatccgtc ttccacttta
     8101 tatccgcccg caaaccatga ccgccgctcc tgatatccta taccgtcaag ccgccgccct
     8161 tttggaacaa tccaataccg cccaagccct gcccctgctg caacaggccg cagagcaggg
     8221 atatacggaa gctgcttttg tattgggcga ccatctgctg caaaacggtc atccggagca
     8281 ggcacttcca tggttggaag ccgccgcggc ccaacgccat cccaaggcac tctttaccct
     8341 gctgcaacaa cgcgaacaca acggcacccc gaccggacag cttcttaacg aatacgcctg
     8401 gctgagtgag caaggtcacc tcgaagccca attaattctc atgcgttacc acacgcagcg
     8461 caacgatcca caatcgctct actgggcgga gcttgccgct gcccaatatg ccgcacctgc
     8521 gtattaccat ttggcacgcc atcatcaacg ccaaggcgac gttgcaacag ctatcgaaca
     8581 atacgaaaaa gcggcagcgc tcggcgtgac tgcagcctgc tggcaactcg gtcaaatcta
     8641 cttctacggc acaggtgtca gccccaacca cgcacaagcc gaacactatc tcgaacaagc
     8701 cgcgcaagcc ggccacatcg ccgcacaaac cctgctggcc gaccttcttg ctgcccaacg
     8761 caaacccgaa gccttagaat ggtatcgtcg tgccgccgac aaagagcaag cggaagcaca
     8821 ggccaagctg gctcaatacg ccctgaccgg cgaactttcc gaacgcgatc cgtttcaagc
     8881 ggcacgatat gccaaagccg ccgctgagaa agaccatccc gaagccctga aaatcatggg
     8941 cgacctctac cgctacggcc tcggcatcaa agctgacaac catatcgcgc acgattacta
     9001 ccaccgtgct gccgcgctgg gttctgccgc cgcagcacaa aaactcatca gcgacgccgc
     9061 gctgtaccat ccgcaacaat acgaacaaat caaaactgcc gccctgcaac agcaacaaac
     9121 cgaaaccatc taccgtttgg cggaaacaca agcccatgcc atcggtcgtc ccgccgacta
     9181 taatgccgca cgcaaaaatt acatggaagc tgccgggttc caccataaaa acgcggcggc
     9241 tgccttaggc cgcatctacc attacggcct cggtacggcg caagatcctc gggcggctgc
     9301 acactggtac gccattgctg ccgaacaaaa ccacccttcc gcccaatacc acctcgcctg
     9361 tttttactat cacgggcaag gtgtcggctg tcatgttccg accgcctgct actggctgca
     9421 ggccgccatc ggcaacggcc acacttcggc cgaatcatta atatccctat tagaacaatg
     9481 gcgacgcgaa gcacaccatg ccatcggaca aaaggccgtc tgaaaagatt tacactcgca
     9541 ttttttgaca atctttaact attcccctaa tatttgccag ttatttttca cggacacgcc
     9601 attgttttca tttctttctg aaaacacctt gtccgcgcat caataccatg acactcggcg
     9661 gataacgcca agcgttgaaa cacactacat ccggaacaaa aacggatgct cggaaaaata
     9721 tttctaggag gtgaaacaac atggaatggg aattcaacag ttattacaca ctgattgccg
     9781 ccacgctcgt gttgctggtt ggtaaatttc tggttcaaaa aatcaaattc ttacgagact
     9841 tcaatattcc cgagccggtc gctggcggtt tggttgccgc gattatcctg tttgcgctgc
     9901 acgaggcgta cggcgtgagc ttcaaatttg agaaaccgct gcaaaatgcg tttatgctga
     9961 tttttttcac gtccatcggc ttgagcgcgg atttttcccg tttgaaggcg ggcggtttgc
    10021 cgctggtggt ttttaccgcg attgtgggcg gatttatctt ggtgcaaaac tttgtcgggg
    10081 tcggactggc tacggctttg ggtttggacc cgctcatcgg tctgattacc ggttcggtgt
    10141 cgctgacggg cggacacggt acgtcaggtg cgtggggacc taattttgaa acgcaatatg
    10201 gcttggtcgg cgcaaccggt ttgggtattg catcggctac tttcgggctg gtgttcggcg
    10261 gcctgatcgg cgggccggtt gcgcgccgcc tgatcaacaa aatgggccgc aaaccggttg
    10321 aaaacaaaaa acaggatcag gacgacaacg cggacgacgt gttcgagcag gcaaaacgca
    10381 cccgcctgat tacggcggaa tctgccgttg aaacgcttgc catgtttgcc gcgtgtttgg
    10441 cgtttgccga gattatggac ggcttcgaca aagaatacct gttcgacctg cccaaattcg
    10501 tgtggtgtct gtttggcggc gtggtcatcc gcaacatcct cactgccgca ttcaaggtca
    10561 atatgttcga ccgcgccatc gatgtgttcg gcaatgcttc gctttcgctt ttcttggcaa
    10621 tggcgttgct gaatttgaaa ctgtgggagc tgaccggttt ggcggggcct gtaaccgtga
    10681 ttcttgccgt acaaaccgtg gtgatggttt tgtacgcgac ttttgttacc tatgtcttta
    10741 tggggcgcga ttatgatgcg gcagtattgg ctgccggcca ttgcggtttc ggcttgggtg
    10801 caacgccgac ggcggtggca aatatgcagt ccgtcacgca tactttcggc gcgtcgcata
    10861 aggcgttttt gattgtgcct atggtcggcg cgttcttcgt cgatttgatt aatgccgcga
    10921 ttctcaccgg ttttgtgaat ttctttaaag gctgattttc cgcctttccg ccaaagcacc
    10981 tgcaaggttt accgcctgca ggtgcttttg ctatgatagc cgctatcggt ctgcaccgtt
    11041 tggaaggaac atcatgtatc ggaaactcat tgcgctgccg tttgccctgc tgcttgccgc
    11101 ttgcggcagg gaagaaccgc ccaaggcatt ggaatgcgcc aaccccgccg tgttgcaagg
    11161 catacgcggc aatattcagg aaacgctcac gcaggaagcg cgttctttcg cgcgcgaaga
    11221 cggcaggcag tttgtcgatg ccgacaaaat tatcgccgcc gcctacggtt tggcgttttc
    11281 tttggaacac gcttcggaaa cgcaggaagg cgggcgcacg ttctgtatcg ccgatttgaa
    11341 cattaccgtg ccgtctgaaa cgcttgccga tgccaaggca aacagccccc tgctgtacgg
    11401 ggaaaccgct ttgtcggata ttgtgcggca gaagacgggc ggcaatgtcg agtttaaaga
    11461 cggcgtattg acggcagccg tccgcttcct gcccgtcaaa gacggtcaga cggcatttgt
    11521 cgacaacacg gtcggtatgg cggcgcaaac gctgtctgcc gcgctgctgc cttacggcgt
    11581 gaagagcatc gtgatgatag acggcaaggc ggtgaaaaaa gaagacgcgg tcaggatttt
    11641 gagcggaaaa gcccgtgaag aagaaccgtc caaacccacg cccgaagaca ttttggaaca
    11701 caatgccgcc ggcggcgatg cgggcgtacc ccaagccgca gaaggcgcgc ccgaaccgga
    11761 aatcctgcat cctgacgacg gcgagcgtgc cgataccgtt accgtatcac ggggcgaagt
    11821 ggaagaggcg cgcgtacaaa accagcgtgc ggaatccgaa attaccaaac tttggggagg
    11881 actcgatacc gacgtgcaaa aagagttggt cggcgaacaa cgcaagtggg cgcaggaaaa
    11941 aatcagcaac tgccgacaag ccgccgcgca ggcagaccgg caggaatacg ccgaatacct
    12001 caagctgcaa tgcgacacgc ggatgacgcg cgaacggata cagtatcttc gcggctattc
    12061 catcgattag gggcaaaccg atgactaccg tccccaaaag caggattccc gtcaaaccgc
    12121 tgcccgaaaa aaccacagac gaagccaaag tcgaaaaatg gcggcagctc ggtgtggaac
    12181 acggtttgtc gggcgaatgg gcagttgccg ccagattggg cgagaacggt tttaccgaag
    12241 aacagatgga aaatatcgcc aacctgttcg gcagataaag ggaaaattga cggaaatgcc
    12301 gtctgaaacc ctgttatcgg tttcagacgg cattttgact aatacggtac gcaggcgcaa
    12361 aacagccggc tttttatgtg tttcctatgc tgatgttcca acacacagga cgatacaaaa
    12421 agcgtcgccc tatgtgccgt cctgattcgg aaagggttac gctcctccca a
//
LOCUS       DAWHXZ010000036        12118 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000036 DAWHXZ010000000
VERSION     DAWHXZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.036"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..1316)
                     /locus_tag="WIE35_000654"
     CDS             complement(<1..1316)
                     /locus_tag="WIE35_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ4153605.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELSFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNMAVWTLEKLPQLTGKIAALTDKAELANLADEAASSLLDSLHEWQFHLAE
                     EPSLSLGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGDFGIFIARIEQISAVWDLLSTVPIEGEEP"
     gene            1419..1643
                     /locus_tag="WIE35_000655"
     CDS             1419..1643
                     /locus_tag="WIE35_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153606.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVSAWVAVIWAYLMIESEKNGRY"
     gene            complement(1711..3012)
                     /locus_tag="WIE35_000656"
     CDS             complement(1711..3012)
                     /locus_tag="WIE35_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ4153607.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCTAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPQPAVLSDGIFLPRNGGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKNTSLMGAYLSAGCISPRLLARKSLERRLNAWTDNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAAAHKTDPDGT
                     FVRRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            complement(3002..3913)
                     /locus_tag="WIE35_000657"
     CDS             complement(3002..3913)
                     /locus_tag="WIE35_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ4153608.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            4021..4419
                     /locus_tag="WIE35_000658"
     CDS             4021..4419
                     /locus_tag="WIE35_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ4153609.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            4468..5073
                     /locus_tag="WIE35_000659"
     CDS             4468..5073
                     /locus_tag="WIE35_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ4153610.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            5259..7196
                     /locus_tag="WIE35_000660"
     CDS             5259..7196
                     /locus_tag="WIE35_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ4153611.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGAVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHRNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNY
                     AWQQTDNKTLSSNFTLNGDYTIGRFENHLTVGMDYSREHRNPTLGYSRAFTASIDPYD
                     RASWPASGRLQPILTQNRHKADSYGIFVQNIFSATPDLKFVLGGRYDKYTFNSENKLT
                     GSSRQYSGHSFSPNIGAVWNINPVHTLYASYNKGFAPYGGRGGYLSIDTSSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDAQNNPYTWAVGGKHRSRGV
                     ELSAIGQIIPKKLYLRGSLGVIQAKVVEDKENPDRVGIHLNNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYNSRN"
     gene            7201..7371
                     /locus_tag="WIE35_000661"
     CDS             7201..7371
                     /locus_tag="WIE35_000661"
                     /inference="COORDINATES: protein motif:HMM:NF012801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ4153612.1"
                     /translation="MTTLPGFARVDAMLGWNHKNVNITFAAANLLNQKYWRSDAMPGA
                     PRTYTARVNYSF"
     gene            7574..7675
                     /locus_tag="WIE35_000662"
                     /pseudo
     CDS             7574..7675
                     /locus_tag="WIE35_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            7877..8419
                     /locus_tag="WIE35_000663"
     CDS             7877..8419
                     /locus_tag="WIE35_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ4153613.1"
                     /translation="MLHLKDKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFS
                     KTPEAIFRCNLHTELKKKRKLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVV
                     LDVKQKGVDMRIGLDISSITLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWN
                     HVPPDLMEHIDGLKTKCPKP"
     gene            8590..9285
                     /locus_tag="WIE35_000664"
     CDS             8590..9285
                     /locus_tag="WIE35_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ4153614.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSVQSSSSAPAAPVE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(9616..10986)
                     /gene="ffh"
                     /locus_tag="WIE35_000665"
     CDS             complement(9616..10986)
                     /gene="ffh"
                     /locus_tag="WIE35_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ4153615.1"
                     /translation="MLDNLTGRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVVGSLTPDQAFIGVVNKTLTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
     gene            11207..12013
                     /locus_tag="WIE35_000666"
     CDS             11207..12013
                     /locus_tag="WIE35_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ4153616.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGLGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
BASE COUNT         2828 a         3083 c         3265 g         2942 t
ORIGIN      
        1 ggctcttcgc cctcaatggg aacggtagag agcaaatccc aaaccgcgct gatttgttcg
       61 atacgggcga tgaagatgcc gaaatcgccg gtcaggcggt cgaggagcgc gccgtcctgt
      121 tctttttcgc ggcgcgcggc ggaaagcgca tcgttcagcc cgacaacgtg tttgagcagg
      181 ctgcgcgcag caatggccgt attggaaacg gtggtttcga ggccttcggg gattttaccg
      241 tcttcccaca gccaagtcgg ttcgctgttg gtccgcctgt cgttttcaga cggccccaag
      301 ctcaaagacg gctcttccgc caaatggaac tgccattcgt gcaggctgtc gagtaaggat
      361 gaggcggctt cgtcggcaag gttggcaagt tcggctttat cggtcagcgc ggcaatcttg
      421 ccggtcagct gcggcagttt ttccagcgtc caaacggcca tattccatga atgttcggcg
      481 gcaaaacggc tgagggcttt tttgggcagg tggtgcgctt cgtctatgca atagaaactg
      541 ttttcgggcg caggcagaat cacgccgccg cccatactga tgtcggcaag cagaagatcg
      601 tggttggcaa cgacgacatc gacggtttcc aagacatcgc gtgccaggta aaacggacat
      661 tccggacggt tgggacaggc ggttttcagg cagccgtggc ggtcgttggt cactttgagc
      721 caaatcgcgt cgtcgatttt ttccggccag gcgtcgcggt cgccattgaa ccgtcgggca
      781 gaaaattcgt cggcaatgtc gcgcagcagc ttcaattctt cgggcttggg tttgctgtcc
      841 cacaagacgg cgggggcttc aaagccgagc aggttttgct gggcattgct ttgcgtcagt
      901 tgatagagtt tgtaggggca gagatagcgg ccgcgccctt tggcaagcgc gaagctcagt
      961 tccaaaccgc ttttttcgac caaaaacggc aggtcgcggt ctaccaactg ctcctgcaag
     1021 gcaaccgttg cgctgctcac aatcagccgc ttgccgcgcg tctgcgccat gatgccgccc
     1081 gccagcaggt aggcaagcga tttgcccacg ccggtcggcc cttcgattac ggcgatgctc
     1141 tcgccctcgc gcttgggcgg ctcgccgcct tcttcgcgcg ccaacgtccg cgaaaaagcg
     1201 ttggcaaccg ccgcaatcat ttcccgctgc gaagcgcgcg gacggaaacc gggcaggttt
     1261 ttgccgatgt tttggtaatg gtcgcggatg gcgttttttt ctaaatcggt gagcatggcg
     1321 ttttgtacgg cggtagaagt gggcttattt taacattgca cggaagcggt acaatatcgt
     1381 tgtcggaatg gggggtgagg tgaatcgtgc ggacgtggtt ggttttttgg ttgcagcgtt
     1441 tgaaataccc gttgttgctt tggattgcgg atatgttgct gtaccggttg ttgggcggcg
     1501 cggaaatcga atgcggccgt tgccctgtgc cgccgatgac ggattggcag cattttttgc
     1561 cggcgatggg aacggtgtcg gcttgggtgg cggtgatttg ggcatacctg atgattgaaa
     1621 gtgaaaaaaa cggaagatat tgagtcattc ggacgcaatg ccgtctgaaa cggaagttca
     1681 gacggcattt gttttaggtt gccgtgccgc ttagggaata ccggcgacag gatgggcggg
     1741 atagccgtgg gtatcgaccg aacaggcaaa ccgccaaggc gtgtggacgg tgtcggcgga
     1801 caggtgggca agctcgggaa tgtgccgtct gacaaaggtg ccgtcggggt cggttttgtg
     1861 tgcggcggcg gcaatgtcgg ggcaggtgtg ccgtgaggcg gcaagccgcc agttgccttg
     1921 gttgattgct gcatcgaaat cggtcagctg ccgggcaaac catatctcgc cttcgcggcg
     1981 ggggaggttt aaaacgtggc agaaaaaatc cgcgctcaag cgtctcaggg cggggtggag
     2041 gctgccggtt ttgtgcaaac agcgcatcgc ggcatcgata atcggaatgc cggtccggcc
     2101 ctgctgccaa agcgtcaggc gcagggtgtg ttcaggattg ccgtctgaag ggtcgtcatc
     2161 cgtgtgctgc aaggcaagtt gaaggaaaaa atcgcggcgg atgatgttgt ccgtccacgc
     2221 gttcagacgg cgttcgaggc ttttccgcgc gagcaggcgc ggcgagatgc agccggcact
     2281 caaatacgcg cccatcagcg aagtgttttt gcgcgagggg aaatccttta agacggagta
     2341 ggaatccgcc tgttcgagaa accgccgcca ctgccgccaa gccgccgttt caccgccgtt
     2401 tcgcggcagg aagatgccgt ctgaaagcac ggcaggctgc ggggcggaaa ggttttcggg
     2461 gaagggttgg cggtatgccg cgaataggtc cggaccggcg gggggctgct tggaaaagcg
     2521 gtcgagccat acttcgcggt agcggtcgaa atcggtgtgt gccgtgccgc cgtcgggtat
     2581 caggtcggtt ttgccgaaaa cggcgcggtc attgacgaag gttaacgcga tgccgtgttt
     2641 gtccaattca tgccaaaggg cgttgtcggc gagtttgtcg gcaaaagtat gggattcgtc
     2701 ggcgatgacg gcgcggatat tgaggcggac ggcgagccgg acgagctcgg caggcgatgc
     2761 cgccgtgtag agcgggatgc cgcgccctgc aagcccttgg gcgagttcgg cggcggattg
     2821 gcggtagaac gcggcgcggc gagggttgtc tgtttcggca tcgtcaatcc aaataccgat
     2881 aacgggcaaa ccttcggcaa cagcggtgca taaggcggcg ttgtcgcgga tgcggaggtt
     2941 ttggcggaac cagacgagcg tgtgtgcggc gcacgtgtcc gcataaaggg ggcgggcggt
     3001 ttcagacggc atttcggcag cctttcctgc tggcgatttt ttcgttcaga aaatcgatga
     3061 agctgcggac tttcgcgctt aagaatgccc tgtctgcata aacggcattc agccggtcgg
     3121 tcgggacggc gtatccgggc agcagcctca ccagcgtgcc gcagcgcaaa tcgtgttccg
     3181 ccgcccaaag cggctgataa ccgatgcacg cgcccgcctt aatcatttcg cgcatcatca
     3241 gcgtgttgtc ggtacggatg acgggggtca gttcaagccg gtattttttg ccgtccgatt
     3301 tgcgggtgag gtcgagtttc tgctggttgg tgtaggtcgg caggacggcg ggcagccccg
     3361 ccacttcttc cggcgtttcc ggcacgccgt tgcgcctcag gaagtcgggc gaggcaagca
     3421 gggcaaattc gatttccgcc agtgggcgcg caatcagtga cggggacagg gtttgggaaa
     3481 cgcgcaacgc caaatccacg ccttcggcaa tcaaatcgac gtggcggttg tccaaaatca
     3541 gttctaatgt cacttcggga taacgttcgc ggtattccgc cagccagttg catatctggc
     3601 tgccggcaaa ccacagtggc atcgttacgc gcagcagccc ctgcggtttt tccgtccccc
     3661 cggcggcttt ttgcgcggca tcgtcgagcg tgtcgagcgc gtaactgcat tgccggtagt
     3721 attcttcccc ggcttcggtc aggctgaggt tgcggctgtt gcggtgcagg agtttggctt
     3781 ggacggtgtt ttccaagtgg ctgacgtgtt tgcttgccat tgcggtggag atgccgagcg
     3841 cgtcggcggc gcgggtgaag ccgccgcttt ggacgacttg gcggaaaacc ttgaggctga
     3901 acagggtgtc catattttct tgtgtggaaa agttgtatca ataaaagcag tatatatttg
     3961 aaaaggggaa acatctatac tctaccgcct gaaatgaaga caaatatcaa aggagctttt
     4021 atgtccgatt gctgcaaccg tatccaaccg gttttgcttt ctgttttgcg tatcgtaacc
     4081 gcttacctgt ttttgttgca cggtacgtcg aaaatcttcg ccttccccat tgaaatgggc
     4141 agcggttcgc ccggcgggct gttgctgctt gccggtattt tagaaattgt cggcggcatt
     4201 ttgctggtgt tgggcctgtt tgcgcgccct gccgcgtttg ttttgtccgg ccagatggcg
     4261 gttgcctatt ttatggcgca cgcttccgga aatgctttgt tcccgattgc caacggcggc
     4321 gagtccgcag tgctgttctg cttcgtattc ctctatatcg cggcggcggg cggcggagca
     4381 tggtcgctgg acaggctgtt tttcaagcgt aaagcctgaa tcggactgcc taaagtgtat
     4441 tttgttgaat gtttttgagg aaaagaaatg acccgtcaat ctctgcaaca ggctgccgaa
     4501 agccgccgtt ccatttattc gttaaataaa aatctgcccg tcggcaaaga tgaagttgtc
     4561 caaatcgtcg aacacgccgt tttgcacaca ccttcttcgt tcaattccca atctgcccgc
     4621 gtggtcgtgc tgtttggcga agagcatgat aaggtgtggc aatttgtcga agacgcgctg
     4681 cgtgccgtcg tgcctgccga cagttttgaa ccgaccgcgc aaaaattgaa cctgtttaag
     4741 gcgggtgcgg caaccatttt gttttatgaa gatcaaaatg tcgtcaaagg tttgcaggag
     4801 cagttccctg cttatgccgc taacttcccc gtttgggcgg accaggcaaa cgcgatggtg
     4861 cagtatgccg tttggacgac acttgccgcg gtcggcgtag gtgcaaacct gcaacattac
     4921 aatcccttgc ccgatgcggc gattgccaaa gcgtggaata tccccgaaaa ctggttgttg
     4981 cgcgcacaaa tggttatcgg cggtattgaa ggggcggcag gtgaaaagac ctttgaacca
     5041 gttgcagaac gtttgaaagt gttcggcgca taatttcgcg gtcaaaaaaa tgccgtctga
     5101 accctgttca gacggcattt ttcagtatca ggcggcgagt tttccgcatt ctgagacctt
     5161 tgtttacaaa tatcatgttc aatatagtta aaagaaatta ttctcatttc ctccgtgagg
     5221 caatataatt cggttgtttt gttaaattga gtataaaaat gaaaatatca tttcatttag
     5281 ctttattacc cacgctgatt attgcttcct tccctgttgc tgccgccgat acgcaggaca
     5341 atggtgaaca ttacaccgcc acgctaccta ccgtttccgt ggtcggacag tccgacacca
     5401 gcgtactcaa aggctacatc aactacgacg aagccgccgt tacccgcaac ggacagctca
     5461 tcaaagaaac gccgcaaacc atcgatacgc tcaatatcca gaaaaacaaa aattacggca
     5521 cgaacgattt gagttccatc ctcgaaggca atgccggcat cgacgctgcc tacgatatgc
     5581 gcggcgaaag cattttcctg cgcggttttc aagccgacgc atccgatatt taccgcgacg
     5641 gcgtgcgcga aagcggacaa gtgcgccgca gtactgccaa catcgagcgc gtggaaatcc
     5701 tgaaaggccc gtcttccgtg ctttacggcc gcaccaacgg cggcggcgtc atcaacatgg
     5761 tcagcaaata cgccaacttc aaacaaagcc gcaacatcgg tgcggtttac ggttcgtggg
     5821 caaaccgcag cctgaatatg gacattaacg aagtgttgaa caaaaacgtc gccatccgtc
     5881 tcaccggcga agtcgggcgc gccaattcgt tccgcagcgg catagacagc aaaaatgtca
     5941 tggtttcgcc cagcattacc gtcaaactcg acaacggctt gaagtggacg gggcaataca
     6001 cctacgacaa tgtggagcgc acgcccgacc gcagtccgac caagtccgtg tacgaccgct
     6061 tcggactgcc ttaccgcatg gggttcgccc accggaacga ttttgtcaaa gacaagctgc
     6121 aagtttggcg ctccgacctt gaatacgcct tcaacgacaa atggcgtgcc caatggcagc
     6181 tcgcccaccg cacggcggcg caggattttg atcatttcta tgcaggcagc gaaaatggca
     6241 acttaatcaa acgtaactac gcctggcagc agaccgacaa caaaaccctg tcgtccaatt
     6301 tcacgctaaa cggcgactac accatcggcc gttttgaaaa ccacttgacc gtaggcatgg
     6361 attacagccg cgaacaccgc aacccgacct taggttacag ccgcgccttt actgcttcca
     6421 tcgatccata cgaccgagca agctggccgg cttcgggcag attgcagcct atcctcaccc
     6481 aaaaccgcca caaagccgac tcctacggca tctttgtgca aaacatcttc tccgccacgc
     6541 ccgatttgaa attcgtcctc ggcggccgtt acgacaaata cacctttaat tccgaaaaca
     6601 aactcaccgg cagcagccgc cagtacagcg gccactcgtt cagccccaac atcggcgcag
     6661 tgtggaacat caaccccgtt cacacacttt acgcctcgta taacaaaggt ttcgcgcctt
     6721 atggcggacg cggcggctat ttgagcatcg atacgtcatc ttctgccgtg ttcaacgctg
     6781 accccgagta cacccgccaa tacgaaaccg gcgttaaaag cagttggctg gacgaccgcc
     6841 tcagcaccac attgtccgcc taccaaatcg aacgcttcaa tatccgctac cgccccgacg
     6901 cacaaaataa tccctacact tgggcagtcg gcggcaaaca ccgttcgcgt ggcgtggaat
     6961 tgtccgccat cgggcaaatc atccccaaaa aactctatct gcgcggttcg ttgggcgtga
     7021 ttcaggcgaa agtcgttgaa gacaaagaaa atcccgaccg agtgggcatc catttgaata
     7081 acaccagcaa cgttaccggc aacctgtttt tccgttatac cccgaccgaa aacctctacg
     7141 gcgaaatcgg cgtaaccggt acaggcaaac gctacggtta caactcaaga aattaaagaa
     7201 gtgactacgc ttccaggctt tgcccgagtt gatgccatgc ttggctggaa ccataaaaat
     7261 gttaacatta cctttgccgc agccaatctg ctcaatcaaa aatattggcg ttcggatgcc
     7321 atgcccggcg cgccgcgcac ttatacggcg cgggttaatt acagtttcta atcccgttcg
     7381 tgcaacagaa tgccgtctga aaatcctgat aatccgtaaa ttcagcacac gaactttttg
     7441 acaggcttta tctacatcag tataatccaa aaagaaatag cgcccttggc agtacgcatt
     7501 acccattaaa tcatagcggg tatgccgatc ccaagggctt ttttatatct ttgacaaata
     7561 taggggaagc aatatgaaaa ccgccatttt gattgacggt gcatttttta ttaagcgggt
     7621 acggtcatta ggcctgtctc aaaatccaca cgatgcaaaa agaattgctg atttgagacc
     7681 tttgcaaaaa tagtctgtta acgaaatttg acgcataaaa atgcgccaaa aaattttcaa
     7741 ttgcctaaaa ctttcctaat attgagcaaa aagtaggaaa aatcagaaaa gttttgcatt
     7801 ttgaaaatga gattgagcat aaaattttag taacctatgt tattgcaaag gtctcgattt
     7861 ggctttccga tattcgatgc tgcatctgaa agataaaatc aacggcacag atcaaatgga
     7921 tgatttatat agaattttct tttacgactg cgtgccgttt gaaaagaaaa tgcacaatcc
     7981 cgtatctcat cgtgccatag atttttcaaa gactccggaa gccatatttc gttgcaatct
     8041 gcataccgaa ttgaagaaga agcgtaaatt agcgttacgt ttaggcaagc tgtcggacaa
     8101 tacagcatgg atattaaaac cccaagtcat gaaaaatctt ctgaaaaacc cgtcaactca
     8161 aattacggaa aacgatgtcg tgctcgatgt taaacaaaaa ggtgtagata tgcgtatagg
     8221 cttggatatt tcatctatta ccttaaaaaa acaagccgat aaaatcatct tgttttctgg
     8281 tgattccgat tttgtcccag cagccaaatt agccagacgg gaaggtatcg attttattct
     8341 tgatcctatg tggaatcatg tcccacctga cttaatggaa cacatcgacg ggttgaagac
     8401 aaaatgtccg aaaccgtaaa ttactagaac aaaggccgtc tgaaaataag gctaattatc
     8461 taagccatat ttcagacggc ctttataaag gctgaaataa aactaaacac attttttcac
     8521 tcaaatcgaa cacgccttca ataaaatgcc ataaaatccg cacattaatc tgacacacaa
     8581 gagataccta tgaaactgaa aaccttagct ttgacttcat tgaccctgtt ggcattggcc
     8641 gcttgtagca aacaggctga aaccagcgtt ccggcagaca gcgtccaaag cagctcatct
     8701 gctccggcag cccctgttga gttgaacgaa ggtgtgaact acactgtatt gtctacgcct
     8761 attccgcaac agcaggccgg taaaatcgaa gtattggaat ttttcggcta cttctgcccg
     8821 cattgcgccc atcttgagcc ggtcttgagc gagcacatca aaacgtttaa agacgatacc
     8881 tatatgcgcc gggagcatgt cgtgtggggt gatgaaatga aacctttggc acgtttggcg
     8941 gccgcagtgg aaatggccgg tgaatcagat aaagccaaca gccatatttt cgatgcgatg
     9001 gttaatcaaa aaatcaatct ggccgatacc gataccctga aaaaatggct gtccgagcaa
     9061 acagcgtttg acggcaaaaa agtattggct gcatttgagg ctcctgaaag ccaagcgcgt
     9121 gcggctcaaa tggaagagtt gaccaataaa ttccaaatca gcggcacacc gactgtgatt
     9181 gtcggcggca aataccaagt tgaatttaaa gactggcagt ccggtatgac cacgattgac
     9241 cagttggtgg ataaagtacg cgaagagcag aaaaagccgc aataagttga ggattgaatg
     9301 agtaaaggcc atctgaaaat aggatttcag acggcctttt gtatttaggc tttatagaag
     9361 agatgattgc ttaaagcctt atggttttaa atcagaatat atagcggatt aacaaaaacc
     9421 agtacggcgt tggctcgcct tagctcaaag agaacgattc tctaaggtgc tgaagcacca
     9481 agtgaatcgg ttccgtacta tctgtactgt ctgcggctcg ccgccttgtc ctgatttttg
     9541 ttaatccact ataaatcaga atataaaaca aaaacgccgt ctgaaatttt agacggcgtt
     9601 ttcggtcaaa tcggcttaca aacccgggaa cattcctttc attcccttca ttcctttcgc
     9661 catacgcatc agtttgccca agccgttgcc gctgaacatc ttcatcattt gttgcatttg
     9721 ttcaaactgt ttgagcaatt tgttcacttc ctgtacggtc gtgcccgcac ccattgcaat
     9781 acggcgtttg cggctggctt tgagcagggc agggttggcg cgttcttttg gagtcatcga
     9841 gttgatgatg gcttccactt tgcccatcgc tttttcagcc gttccttcgg ggatttgttt
     9901 cgatatttga cccagttcgc ccggcatttt cgacatcagg ttttccaaac cgcccatatt
     9961 gcgcatttgc tggatttgtt ctttaaagtc gttgaggtcg aagcctttgc ctttttgtag
    10021 ctttttcgcc attttggcgg cggcttcttc atcaatacct ttttggacgt cttcaatcag
    10081 ggtcaatacg tcgcccatgc cgaggatacg gctggcaaga cggtcggggt ggaaaggttc
    10141 gaggccgttg attttttcgc cgacaccgat aaatttaatc ggcttgccgg ttacgtggcg
    10201 cacggacaaa gctgcgccgc cgcgcgaatc gccgtccatc ttggtcaata cgacaccggt
    10261 cagcggcagg gcttcattaa atgcctgagc agtgttcacc gcatcctgac ccagcatcgc
    10321 atcgatgacg aacaaagttt ccaccgggtt aaccgccgcg tgaagggctt tgatttcgtt
    10381 catcatctcc tcatcgattg ccaaacggcc ggcggtatcg accatcaata catcgtaaaa
    10441 atgttttttg gcgtaatcga cggcggcagt tgcaatctca accggttttt ggttggtatc
    10501 ggatgggaaa aagtccacgc cgacctgttc ggccaacaga cgcagttgtt caatcgcggc
    10561 agggcggtaa acgtcggcag atacgaccaa aacctttttc ttctgatcgt ttttcagcag
    10621 gcgggcgagt ttgccggcgg tcgtcgtctt gcccgcgccc tgcaaacctg ccatcaacac
    10681 gacggcgggc ggcgcaaccg acaaatccag cgttttgttt tccctgccca tcagctcggt
    10741 cagggttttg ttgaccacgc cgataaatgc ctgatccggc gtcaggctgc ccaccacttc
    10801 ctgaccgagg gccttttctt tgacgttgtt gacgaactct ttgacgacag gcagggcgac
    10861 atccgcctca agcagggcga ggcggacttc gcgcaaggct tctttaatat tgtcttcggt
    10921 cagtttggcc tgcccgcgga tgtttttgag aacattgctg aagcggccgg ttaaattgtc
    10981 taacatactg gtccttggtc tgaataagaa tagcttgccc catcaggggc attctttgtt
    11041 aaaataaaat caaaataatt tgatgcggct tgtgtgccgg acagcatatc ggcaaatccg
    11101 tcaaggcttg cccgaaacgg ggattttaca attccaacgt taaaagttcc aatatttcat
    11161 aagcggccgc atacagcgca acagtataga ttagagaaag tccaccatgc cgacagtttt
    11221 catctttttg acggcggttt acgcaggatt gggtgcattt gcatggcact gccaacagca
    11281 ggggctcggc cgggattacc cgtggaagac ggaattgccg gttttgggcg cggcattgac
    11341 cgtccatggc gcggcactgc ttatgccggt cattcaagac aaaatcatca ttatgggctt
    11401 tgggtattcc ggcagcctga ttgtttggat gatgctgttt atttattttg ccggcagctt
    11461 cttttatccg ctgcgcggag tgcagttgct gctgtatcct tgcgccgcac tgatgctgct
    11521 gtcaggtttg gtttttcctg gaaaattctc gggatatgaa attaccgacc ttccctttat
    11581 gctgcatatc ggaacttcgc tgcttgcata cgggctgttc ggcatcgcaa cattattgtc
    11641 cgttttgacc ctgctgctga atcggagcct gcaccgcagg aacttctcca agctcgcagg
    11701 attcctgccg tcgctgctca gtttggaaaa actcatgttc caggccatgt gggcaggttt
    11761 catcctgctg acctattccg tcgtcagcgg aacatttttt gccgaagccg tattcggcaa
    11821 acccatgacc tttacccata aaaccgtatt cggcatattg tcatggctga tttacggcgg
    11881 actgctgctc aagcacagca tgaccgcatg gcgcggcaaa aaagccgccg tgtggaccat
    11941 catcggattt gtcagcctga tgattgccta tatgggcagc aagttcgtat tggaaatcat
    12001 tctgaaaaga taagaagagc caacagatgc cgtctgaagc cccgagtttc agacggcata
    12061 ttcacaaagg cgcaccagtc agaggaggga gaggaaggga ttgttggagg cggcgcag
//
LOCUS       DAWHXZ010000037        12102 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000037 DAWHXZ010000000
VERSION     DAWHXZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12102
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.037"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1410
                     /locus_tag="WIE35_000667"
     CDS             <1..1410
                     /locus_tag="WIE35_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ4153617.1"
                     /translation="VKVASLSRFNNSKATKAALEGMADGTVDIVIGTHKLVQDDIKFK
                     NLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGLRDFSLITTA
                     PSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRERLETLLPEAR
                     IGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIINRADKFGLAQ
                     LHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLAMQDLEIRGA
                     GEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEIKLHSPALLP
                     ESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIESHHLRLMAK
                     ELGIDAIDAAGEAVTVTFGKNNNVDPTEIILLIQNDKKYRLAGADKLRFTAEMENIEV
                     RINTVKNVLKTLQNRCLPK"
     gene            1559..1942
                     /locus_tag="WIE35_000668"
     CDS             1559..1942
                     /locus_tag="WIE35_000668"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ4153618.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            1964..2806
                     /gene="kdsA"
                     /locus_tag="WIE35_000669"
     CDS             1964..2806
                     /gene="kdsA"
                     /locus_tag="WIE35_000669"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ4153619.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCTHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPYQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEADNGKL
                     ILCERGSSFGYDNLVVDILGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            2821..3261
                     /locus_tag="WIE35_000670"
     CDS             2821..3261
                     /locus_tag="WIE35_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ4153620.1"
                     /translation="MMQTFRKISRYVATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAILTRLQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSMIFMATALLSAVLSWRLSGKDAV"
     gene            3309..4595
                     /gene="eno"
                     /locus_tag="WIE35_000671"
     CDS             3309..4595
                     /gene="eno"
                     /locus_tag="WIE35_000671"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ4153621.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            4609..4887
                     /gene="ftsB"
                     /locus_tag="WIE35_000672"
     CDS             4609..4887
                     /gene="ftsB"
                     /locus_tag="WIE35_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ4153622.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(4928..5218)
                     /locus_tag="WIE35_000673"
     CDS             complement(4928..5218)
                     /locus_tag="WIE35_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ4153623.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(5416..6549)
                     /gene="nrdB"
                     /locus_tag="WIE35_000674"
     CDS             complement(5416..6549)
                     /gene="nrdB"
                     /locus_tag="WIE35_000674"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ4153624.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(6610..7797)
                     /locus_tag="WIE35_000675"
     CDS             complement(6610..7797)
                     /locus_tag="WIE35_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ4153625.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(7794..8804)
                     /gene="dcm"
                     /locus_tag="WIE35_000676"
     CDS             complement(7794..8804)
                     /gene="dcm"
                     /locus_tag="WIE35_000676"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ4153626.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KK"
     gene            complement(8862..11141)
                     /gene="nrdA"
                     /locus_tag="WIE35_000677"
     CDS             complement(8862..11141)
                     /gene="nrdA"
                     /locus_tag="WIE35_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ4153627.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            11670..11981
                     /locus_tag="WIE35_000678"
     CDS             11670..11981
                     /locus_tag="WIE35_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ4153628.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFHSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
BASE COUNT         3217 a         3089 c         2690 g         3106 t
ORIGIN      
        1 gtgaaagtcg ccagcctttc gcgtttcaac aacagcaaag ccaccaaagc cgcgctggaa
       61 ggcatggcgg acggcacggt cgacatcgtt atcggcacgc acaaactggt gcaggacgac
      121 atcaaattca aaaacttagg tttagtgatt atcgacgaag aacaccgctt cggtgtgcgc
      181 caaaaagagc agctcaaacg cctgcgtgcc aatgtcgaca tcctcaccat gaccgccacg
      241 ccgattccgc gtactttaag tatggcgttg gaaggactgc gcgacttctc gctgattacc
      301 accgcgccca gccgccgcct cgccgtcaaa acctttgtca aaccctttag cgaaggcagc
      361 gtgcgcgaag ccgtgttgcg cgaactcaaa cgcggaggac aggtattttt cctgcacaat
      421 gaagtagata cgattgaaaa tatgcgcgag cggctggaaa ccctgctgcc cgaagcccgc
      481 atcggcgtgg cgcacggaca actgcgcgag cgcgagctgg aacaagtcat gcgcgacttt
      541 ttacagcaac gattcaacgt gttgctctgt tccaccatca tcgaaaccgg tatcgacatt
      601 cccaacgcca acaccatcat catcaaccgc gccgacaaat tcggactggc gcaactgcac
      661 cagcttcgcg ggcgcgtcgg acgcagccac caccaagcct acgcctacct gctcacgccc
      721 gaatacatca ccaaagacgc agaaaaacgc ctcgacgcca tcgcggcagc agacgaactc
      781 ggcgcaggtt tcaccctcgc catgcaggat ttggaaatcc gcggcgcagg cgaaatcctc
      841 ggcgaagggc agtccggcga aatgatacag gtcggcttca cgctctacac cgaaatgctc
      901 aaacaagccg tgcgcgatct caaaaaaggc cgccaacccg acctcgacgc gccgctgggc
      961 atcaccaccg aaatcaaact gcacagcccc gccctactgc ccgaaagcta ctgccccgac
     1021 atccacgagc gtctcgtcct ctacaaacgc ctcgccgtct gcgaaaccgt gcagcaaatc
     1081 aacgccatac acgaagaact cgtcgaccgc ttcggcctgc ccgaacaacc cgtcaaaacc
     1141 cttatcgaaa gccaccacct gcggcttatg gcaaaagaat tgggtatcga tgccattgat
     1201 gcggccggcg aagcggtaac ggtaaccttc ggtaaaaaca ataatgtcga tccaaccgaa
     1261 atcatcctgc tgattcagaa cgacaaaaaa taccgccttg ccggcgccga taagctgcgg
     1321 tttaccgcag agatggaaaa tatcgaggtc agaatcaaca ccgtaaaaaa cgttttaaaa
     1381 accttgcaaa acagatgcct gcccaaataa agccgacacc gcaatgccgt ctgaaacacc
     1441 gttttccttg tccgaaagcc gccattatga atttgaagga aactccacta taatacggca
     1501 ttcagatttc cagacggcat cgcgcccgtc agaccgcaca caaaccaaaa ggaaatacat
     1561 gttccgtact atgcttggcg gaaaaatcca ccgcgccacc gtaaccgaag ccgatttgaa
     1621 ctacgtcggc agcattaccg tcgatcaaga tctgttagac gcggcaggca tctaccccaa
     1681 cgaaaaagtc gccatcgtca acaacaacaa cggcgaacgt tttgaaacct ataccatttc
     1741 aggtaaacgc ggcagcggcg tgatttgtct gaacggtgct gcggcaaggc tggtgcagaa
     1801 aggcgatatc gtcatcatca tgtcttacgt ccaactctcc gaacccgaaa tcgccgcgca
     1861 tgaacccaaa gtcgtcttgg tagacggaaa caacaaaatc cgcgacatca tctcctacga
     1921 gccgccgcac accgtgctgt aaccctgaaa acggacattg atcatggata ttaaaattaa
     1981 cggcattacc gtcggtaacg atgctccttt cgtactgttc ggcggcatca acgttttaga
     2041 agatttggat tccaccctcc aaacctgtac gcattacgtc gaagttaccc gaaaactcgg
     2101 tattccctat atctttaaag catctttcga caaggcaaac cgttcctcca tccattctta
     2161 tcgcggcgta ggcttggaag aaggcttaaa gatttttgaa aaagtcaaag cggaattcgg
     2221 catccccgtc attaccgacg tacacgaacc ctatcagtgc caacccgtcg ccgaagtgtg
     2281 cgatgtcatc cagcttcccg cattccttgc gcggcagacc gatttagtgg ttgccatggc
     2341 aaaaaccggc aacgtcgtca acatcaaaaa acctcagttc ctcagcccct ctcaaatgaa
     2401 aaacattgtg gaaaaattcc acgaagccga caacgggaaa ctgattttat gcgaacgcgg
     2461 cagcagcttc ggctacgaca acctcgttgt cgatatactc ggtttcggcg tgatgaaaca
     2521 gacttgcggc aacctgccgg ttattttcga cgttacccat tccctgcaaa cccgcgatgc
     2581 cggttctgcc gcatccggcg gtcgtcgcgc acaggctttg gatttggcac ttgcaggcat
     2641 ggcaacccgc cttgccggtc tgttcctcga atcgcacccc gatccgaaac tggcaaaatg
     2701 cgacggcccc agcgcgctgc cgctgcacct tttagaagat tttttaatcc gcatcaaagc
     2761 attggacgat ttaatcaaat cacaaccgat tttaacaatc gagtaacacg gtttcgcctt
     2821 atgatgcaga ctttccgaaa aatcagccgg tatgtcgcaa ccttgtggct cggtatgcag
     2881 attatggcgg gttatatcgc cgcaccggtg ctgttcaaaa tgctgcccaa aatgcaggcg
     2941 ggcgaaattg ccggcgtatt gttcgacatc ctctcttgga gcgggcttgc cgtttggggc
     3001 gcggtgttgg ctgccgcctt tgccatcctg acccgactgc aaaccgccct gctgcttttt
     3061 ttattgtccg cccttgccgc caaccaattt ttggttaccc ccgttatcga ggcactgaaa
     3121 tacgggcatg aaaattggct gttgtcgttt gtaggcggat ccttcggaat gtggcacggt
     3181 atttccagca tgattttcat ggcaaccgcc ctactttcag cagttttaag ttggcggctt
     3241 tccggcaaag atgccgtctg aagccctccc atttttttac ctcccttcac ttcacttgga
     3301 gaacattcat gagcgcaatc gttgatattt tcgcccgcga aattttggac tcacgcggca
     3361 accccacagt cgagtgtgat gtattgctcg aatccggcgt aatgggacgc gcagccgtac
     3421 cgagcggcgc gtccaccggt caaaaagagg ctttggaact tcgcgacggc gacaaatccc
     3481 gttattcggg caagggcgta ttgaaggcgg tcgaacacgt caacaaccaa atcgcccaag
     3541 ccctcattgg tatcgatgcc aacgagcaat cttatatcga ccaaatcatg atcgaattgg
     3601 acggtactga aaacaaaggc aatttgggtg cgaatgcgac tttggcggtt tctatggcgg
     3661 ttgcacgcgc cgctgccgaa gactcaggcc tgccgcttta ccgctacttg ggcggcgcag
     3721 gcccgatgtc cctgcccgta ccgatgatga acgtcatcaa cggcggcgaa cacgccaaca
     3781 acagcctgaa catccaagag tttatgatta tgcccgtcgg cgcaaaatct ttccgcgaag
     3841 cgttgcgctg cggtgcggaa attttccacg ccttgaaaaa actgtgcgac agcaaaggct
     3901 tcccgaccac agtcggcgac gaaggcggtt tcgcccccaa cctgaacagc cacaaagaag
     3961 ccctgcaact gatggtcgag gcgaccgaag ccgccggcta caaagcgggc gaagacgtat
     4021 tattcgcatt ggactgcgcc tccagcgagt tctacaaaga cggcaaatac cacttggaag
     4081 ccgaaggccg ctcctacacc aacgcggaat ttgccgaata tctggaaagt ctggtcaacg
     4141 agttccccat catctccatc gaagacggca tggatgaaaa cgactgggaa ggctggaaac
     4201 tgctgaccga aaaactgggc ggcaaagtcc aactcgttgg cgacgacctc ttcgttacca
     4261 acccgaaaat ccttgccgaa ggcattgaaa aaggcgtggc aaacgcacta ttggtcaaag
     4321 tcaaccaaat cggtactttg agtgaaaccc tgaaagccgt cgatctggca aaacgcaacc
     4381 gctacgccag cgtgatgagc caccgctccg gcgaaaccga agacagcacc attgccgact
     4441 tggcagtcgc caccaactgt atgcagatca aaaccggttc tttgagccgt tccgaccgca
     4501 tggcgaaata caaccaactg ctgcgtatcg aggaagaatt ggcggaagcc gccgactacc
     4561 ccggcaaagc cgcattctac caactgggca aataaaaaag gttaaggtat gaagtgggta
     4621 actgtcgttt tatccttcgc acttgtctgt tgccaataca gcctctggtt cggcaaaggc
     4681 agcatcggac gcaacagcag tctgagagaa cagattgccg ttcaagaaga aaaaaaccag
     4741 acactcgccc tacgcaatca ttcccttgcc gccgaagtct atgatttgga aaacggtcaa
     4801 gaagccattt cggaaatcgc ccgggtagaa ctgggttata tccaagacgg tgaaaccttt
     4861 taccgactca tcaggcataa ccggtaatac cgtcaaaaag ccgtccgaac caatgttcgg
     4921 acggctttta tttcaacaaa ctgtcagaca gcccctcatc ctcccccgac aaaccgcaat
     4981 ccagcctgac atccccctcg acgcaacagc agcacggcag tatctcgtcc cgccccaaaa
     5041 aagccaaagg cggctcccga taagtaacgc ttccctccaa aatcttcact cggcacgatc
     5101 cgcaatatcc gcttcggcac tgatattcca ccatatgccc cgtccgttcc aagccttcca
     5161 acagggtctc gccctccaag agctcaaaaa aacccttatt cgtaccaatg cgcgccattt
     5221 ccgaccaatc aaaaatatag tggattaaat ttaaaccagt acggcgttgc ctcgccttgt
     5281 ccggattttt gttaatccac tataatccac tataaaagga acaataaccg atcctacccg
     5341 ctgtttttcc catcatacaa catacaaatg ccgtctgaaa catccggctt cagacggcat
     5401 tttttcaaaa aacatttaca actcaaaatc gcccaaatca tccgtattca cttcagaatc
     5461 tatctgaccg atcaaataag aggatatttc cacttcctgc ggcgcgacct gtacgttgtc
     5521 ggacgacagc cacgcattaa tccacggaat cgggttttga tttgcgcctt caaatccggc
     5581 cggcaacccc accgcctgca tacgcagatt ggtaatatat tcgacgtatt gggataagat
     5641 ttctttgttc aaaccgatca tcgaaccgtc cttaaacaaa tatgccgccc attctttttc
     5701 ctgttccgcc gcttttttga agagttggaa acattcgtcc tgcaactcgg cggcaatttc
     5761 cgccatttca gaatcatcaa caccagaacg catcagatta agcatatgct gcgtgccggt
     5821 caggtgcagg gcttcgtcgc gggcaatcag tttgatgatt ttggcgttgc cttccatcaa
     5881 ctcgcgctcg gcaaaagcaa acgagcaggc gaatgaaacg tagaaacgga tggcttccaa
     5941 cacattgacg cacatcaggc agagatagag ttttttcttc aacccgcgca aagacacggt
     6001 aacgggtttg ccgccgacat tgtgcacccc ttcgcccaac aggttgtaat actgggtgta
     6061 ttcgattaag tcatcgtaat agcaggcaat gtcttcggcg cgggcggtaa tgtattcgtt
     6121 ttcgacaata tcatcaaaca cgaccgacgg atcattcaca atattgcgga tgatgtgggt
     6181 atagctgcgc gagtggatgg tttcgctgaa gctccacgtt tcaatccacg tttccaactc
     6241 gggaatcgaa accaaaggca gcaaagcaac attcggactg cgcccttgga tggaatcgag
     6301 cagtgtttgg tatttcagat tgctgatgaa aatatgtttt tcgtgttctg gcaggttggc
     6361 gtagtcgata cggtcgcgcg acacgtcgat ttcttccggc cgccaaaaaa acgacaattg
     6421 tttttcaatc agtttttcaa atacctcgta tttctgctgg tcataacggg cgacattaac
     6481 cggctgaccg aaaaacatcg gctcattcag cgcgtcgttt ttggttttgg gaaaggtgct
     6541 gtatgacata gttaagtgtt cgcaggacat aatctattct ctatattaat taaaaatgat
     6601 ctgtaagttt taactaacct gctgagactt tatatattta ataaaatttt ctaaagttaa
     6661 aacatgggtt ttcccaaaag ttccaaatgt ttttacggat tctttaggga caactaaggt
     6721 taaattctca tcactcattt cctgaatttg agtatcagat ataccttctt gtagagtaaa
     6781 taaatgctta tgaggaattc gatctgcttc atttaaaact tggcgccagc gatccttaca
     6841 agtagtcttt gaacctagca ttgtcagctt ttcagtttga aatatgaaat tccctgattc
     6901 atccatcgca tggtactctt cacttccagg aaataaaaaa tctggtttct ttttcccttc
     6961 tgtcttcgct tgcgtctcaa attttaagga aaattcgtca aatattcttg ataaatgtaa
     7021 ctctaaagat tttcctgctc ttgcttttct gcgatttgta aaagaatgtg caaaattaat
     7081 gaagctatcc aaatcagtaa tttttgattt aataatttca aattcacgtt tttcaaaaat
     7141 ggaaaataat tcatattcct ttgaaaccca ttgttttaac acattactaa cgttagtttt
     7201 atttaatgca atattttccc ttgccaaaac tgccatttct tcagttttgg ggaagttagg
     7261 aaatagtgaa aataattttg ttaggttatc ctctacttct tgttttttag gtaaatataa
     7321 acttcctggt aaaatgttag tttctgcgat aaatatttca atatcttcat ctgaagataa
     7381 aacaaaagca tggaataata aatctttata acaagctctg cacaatacca ataaggaacc
     7441 actatattta tcacttaaaa attcaaaatt cttgccaaaa cccgtaattc tcgcctcatt
     7501 tctcgtgcct tggccgtaat atttaaaatt gctattagtg acgcttccat cttgccaatt
     7561 aattttgatt gaaatagttt tatttgttcc cttttggcaa actacatcaa agaggtcgtc
     7621 tgaaaaatgt ttttgaatat aaaatcctga ttggtgacta ccagtagtac caacatcatt
     7681 gggacgaata taacgacagt atactgcaat tgatttattt gctgtctgta ttgctgaagc
     7741 tactaagtca tcggatttat ttactacttg tattgtctga gctgccaagt cattcatttt
     7801 tttaactcat ttataatttg tttagcaata gcttgaacca acggtacagc aattgaattg
     7861 ccaaactgct tgtatgcagc tgtcttggat actgcatcaa taacaaaatc tttaggaaat
     7921 cccattaaac gcgagcactc cctaggtgtc agcttcctag gattttttcc tttctgaggg
     7981 atgagtattt cggaaccatc tttgtaatat cgtgcagata gagttcgtga tattccatct
     8041 aaatcaacta atccaaaacc aaatccatta ccctttgcct tatgtttttt agcgtaattt
     8101 tgaaggtaaa gccataagtt atcagaaaga gtaaaagaat tatctacatc atcttccaaa
     8161 atttgcttta attttggttg tgattctggt ggggaaggaa aattgaaatt tatttcctta
     8221 ttaaaatatt gtctatcaaa acctacaata aaaatacgct ccctattttg aggaacataa
     8281 tattttgcat tcataacttg ataaaatatc tgatagtcaa gctcttctaa agtcccttta
     8341 attactttaa atgtatttcc tttgtcatgc gaaacaaggt ttttcacatt ctctaaaaga
     8401 aaaattttag gtcgatgttt tccaataatt tcagcaacat caaaaaatag agttccctgc
     8461 gccttatcta agaagcctgt ttctcgtcct aggctttttt tctttgaaac accagctata
     8521 gagaatggct gacacgggaa tcctgctgtt aatacatcaa acttacttgg aatagctgct
     8581 ttggtttcct ttaatgtaat atctccataa ggaatatcat taaaatttac ttggtaggtt
     8641 tgacgggctt tatcatccca ttcactagaa aatacacatc gcccaccaac attctccatt
     8701 gcaatgcgaa aaccacctat tcccgcaaat aaatcaataa aagtaaattt ctcattattt
     8761 aaggtaacta tattatctaa ttgattttgt attttatttt tcatatttta tatttcagat
     8821 gatttattaa tctaaaggcc atctgaaaac tccccctttc atcaaatctt acaagccccg
     8881 cccgcgcagc cgtcatcctg aatatcggtc tgcgtatcgt ccgcaccgtc gcgggtgtta
     8941 tggtagtaca gggttttgac gccgtatttg taggcggtca gcaggtcttt gagcatttgt
     9001 ttcatagaaa ctttgccgcc ttcgaatttg cccgggtcgt aggcggtatt ggcggaaatc
     9061 gattgatcga cgaatttttg catcacgccg acaagtttca ggtagccttc gttgccggga
     9121 agctgccaca gggtttcata ggcatttttc agggtttcaa actccggcac gacttgtttc
     9181 aaaatgccgt ctttcgatgc tttgaccgtt accaatccgc gcggcggctc gatgccgttg
     9241 gtggcgttgg cgatttgaga gctggtttca gacggcatga gcgcggtcag agtagagttg
     9301 cgcaggccgt atttgacgat ttcggcacgc aggctttccc agtcgtaatg caaaggctcg
     9361 ccgcagacgg catccaaatc ttttttgtag gtgtcgatgg gcagtttgcc ttgcgaataa
     9421 acggtttggt taaagagcgt acacgcaccg tattctttgg caaggtttgc cgatgctttg
     9481 agcaggtaat actgtatggc ttcaaaggta cggtgggtca gaccgagcgc ggagccgtcg
     9541 ctgtagcgga caccgttttt cgccagataa taagcatagt taatcacgcc gataccgagc
     9601 gaacggcggc ccatagtgga ggtacgcgcg gcttctaccg gatatccctg ataatctaaa
     9661 agtgcatcga gtgcgcgcac agtcaaatcg gcaagccctt ccaattcgtc caggctgttt
     9721 aatgcgccca agttgaaggc ggacagggta cacagggcga tttcgccgtt cggatcgttg
     9781 atattgtcca gcggtttggt cggcagggcg atttccatac acaagttgga ctgatgaaca
     9841 ggcgcgacgc gcggatcgaa cgggctgtgc gtattgcagt gatcgacgtt ttgaatgtag
     9901 atgcgcccgg ttccggcacg ctcctgcatc agcgtggaaa acaggtcggc agccggaatg
     9961 atgcgcttgc ggatatcagg gtcttgctcg tatttcgtat agagccgctc aaattcgtct
    10021 tggtcggcaa aaaacgcttc gtacaatccc ggaacctcgt tgggcgaaaa cagcgtaatg
    10081 ttgccgccct taatcaggcg ggtgtacagt aggcggttga tttgcacacc gtaatcaagc
    10141 tggcggatac ggttgtcttc cacgccccgg ttgtttttca acaccagcag gctttcggct
    10201 tcgatatgcc acaaggggta gaacaaggtt gccgcgccgc cgcgcacgcc gccttgcgaa
    10261 caggatttga ccgccgcctg aaacattttg aaaaacggga tgcagccggt atgccgcgct
    10321 tcgccgcccc ggatttcgct gtccaaaccg cggatacgtc cggcattgat gccgatgccc
    10381 gcacgctggg aaacgtattt cacaatcgcg ctggtagtgg cattgatgga atccaaacta
    10441 tcgtcgcatt caatcagcac acagcttgag aactggcgcg taggcgtacg cacgccgctc
    10501 ataatcgggg tcggcagcga tactttaaat gtggaaacgg catcgtaaaa ccgtttgacg
    10561 taatcgaggc gtgtttcttt gggatatttg ctgaaaagac acatcgccac cagaacatat
    10621 aagaactgcg gtgtttcata aatctggcgg gtaacgcggt tctgtaccag atatttgcct
    10681 tcgagctgtt tgacagcggc ataggaaaag gacatatcgc gttcgtggtc gatataggcg
    10741 ttcagctcgt caaattcttc gcggctgtaa tcctcaagga tatgcctgtc gtattttgcg
    10801 gcatcggtaa gttttttaac gtggtcgtaa aggtgcggcg gctcgtactc gccgtaggct
    10861 attttacgca gatggaaaat cgccaaacgc gcggcaaggt attggtagtc cggggtatct
    10921 tccgaaatta aatcggcagc ggctttgatg atggtttcgt ggatgtcgtc ggtgcggatg
    10981 ccgttgtaga actggatgtg cgatttcaac tcgacctgag atacggaaac attttccaat
    11041 ccgtccgccg cccaagtgac gacacggtga atcttatcca aatcaatggc ttctaatctt
    11101 ccgtctcgtt tggttacttt caaatcagtc ggtgtattca tcgcttcctc ttccactctt
    11161 gatattcaag acacggtctt ttcaaataaa ttaaggcaga caatatagtg gatttttggc
    11221 attttctcca gtcttgacaa cggttgtatt tttcagtttc aggcaatgcg cgataaaacc
    11281 cgcctgtcgt atttttccta taatatttgt tttatctgat aattctttac cgataaaaaa
    11341 cgggtaaatt ttttgccttt tgaccggctc cggctacaag gcggtgaaat aaggattttc
    11401 cgacgaaaaa ggaaagcttc ctgttttctg cccctgcaaa ttgttaaatt ttgcaaagta
    11461 tgattttgcc gcgccgccgc cgacaaattc cattttctta ccgattggaa tttattattg
    11521 agattaatgt gttatttgaa tctgcatatc aaacggcaag ttttgtcggc tgaatgagtc
    11581 tgaaactgcc gacaatttgc ccgctccatc cttccatcct atactggaag gaatgacaaa
    11641 ctgaaaggat cgtcatgtct gtcaaaaaga tgcttgccat actgttgtct gcaatattgg
    11701 gactggtatc gacaactgcc gctgccggta cgtcagaacc cgcacaccgc gataccaaac
    11761 atatccgcaa ggcaaacaag cagatgctgc accccgaatg caggaaatat ttggaacgcc
    11821 gtgccgcgtg gtaccgatcg caaggcaacg tgcaggaatt gcgcgaaaac aaaaaggcgc
    11881 gcaaagcatt ccactccctg ccttatgcgg aacagaaaat ccaatgccgg gcggcttatg
    11941 aggctttcga tgatttcgac ggcggcagtt tccgccgtta atcccatata aaatatgccg
    12001 tctgaacaca ggttcagacg gcattttcca tagatacaac aaactatcct tacgccattt
    12061 cgtttgaggt cctcccgttg ccgcagcctt aatatagtgg at
//
LOCUS       DAWHXZ010000038        12089 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000038 DAWHXZ010000000
VERSION     DAWHXZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12089
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.038"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            348..5000
                     /gene="iga"
                     /locus_tag="WIE35_000679"
     CDS             348..5000
                     /gene="iga"
                     /locus_tag="WIE35_000679"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ4153629.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADEIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLAGNERGANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGINN
                     DITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQKADSNQKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTGHTSTITLTGKSLITNPNSLSVHSIQNDYDEDDYSYYYRPRRP
                     IPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNN
                     QHISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQSASFSSGRNVS
                     DITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGNV
                     NLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANQYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVQDRSRLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQTDSAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQQAEA
                     EKVAHQKAEEAKRQQDALARQQAEQERQRLEAERQAAEIAKQKAEAEEAKRRAAEIAE
                     QKAAAEEAKRQAAELARQQEEARKAAELAAKQKAETERKAAEIAEQKAEAEREAAELA
                     KQKAEEEGRQAAQSQPKRRNRRAIPPELSSDATTRALPRIARNSNPDASDYEEIPLDA
                     LEDEDVSESVDTSDKQPQDNTELHEKVETVSLQPRAAQPRAQAAAQPQAQAVAQADAV
                     STNTNSALSDAMASTQSILLDTGASLTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQ
                     YRRFSSKRTQTQIGIDRSLSENMQIGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYL
                     NDAWYVAGDIGAGSLRSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYS
                     RLSSADYKLGNDSVKVSSMSVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGV
                     NVGGNSFAYKADNQQQYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(5179..6381)
                     /gene="trpB"
                     /locus_tag="WIE35_000680"
     CDS             complement(5179..6381)
                     /gene="trpB"
                     /locus_tag="WIE35_000680"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ4153630.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPS
                     FWEEFHRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     EQMLEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGSPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(6464..7153)
                     /locus_tag="WIE35_000681"
     CDS             complement(6464..7153)
                     /locus_tag="WIE35_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ4153631.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAVYRAF
                     FEMAGLCEKQL"
     gene            7350..8129
                     /gene="rsmA"
                     /locus_tag="WIE35_000682"
     CDS             7350..8129
                     /gene="rsmA"
                     /locus_tag="WIE35_000682"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ4153632.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            8302..9030
                     /locus_tag="WIE35_000683"
     CDS             8302..9030
                     /locus_tag="WIE35_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ4153633.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            9103..9462
                     /locus_tag="WIE35_000684"
     CDS             9103..9462
                     /locus_tag="WIE35_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153634.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            9467..9913
                     /locus_tag="WIE35_000685"
     CDS             9467..9913
                     /locus_tag="WIE35_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ4153635.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLKITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAATLAGSSMVTPYALF
                     LQWDTGGKTVRHCIMPDMTDKESYRKLKVWVLWRQPKKTAETDTSD"
     gene            9944..11218
                     /gene="folC"
                     /locus_tag="WIE35_000686"
     CDS             9944..11218
                     /gene="folC"
                     /locus_tag="WIE35_000686"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ4153636.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERTSEVKKRMKLEPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARSEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPESLLSHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDDPARNRNALPFPALRGTYQLSNAACTLTVLECLNAKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLETVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            11231..>12089
                     /gene="ftsN"
                     /locus_tag="WIE35_000687"
     CDS             11231..>12089
                     /gene="ftsN"
                     /locus_tag="WIE35_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ4153637.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESRKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAVGID"
BASE COUNT         3532 a         3177 c         2907 g         2473 t
ORIGIN      
        1 acgaaccccc atctcgtcat taccacgaac ccccatctcg tcattaccac gaaagcggga
       61 atctaggacg cagggttaag aaaacctaca tcccgtcatt cccgcacaag cgggaatcca
      121 aaaacaaaaa gtcgcagaaa tttaacggaa gaaccgaacc ccgaaaatcg gcttcccgcc
      181 ttcgcttgaa taacagaaca aaggaagcgg aaataaaaac atcgggattc tttttttaaa
      241 ttgacaataa aacaccaaaa ttaatgattt acacaacaaa aaatagtcaa caccgccaaa
      301 ccggcgtaaa atacaagaca ttatcaccct taaaacggta aaaccttatg aaaaccaaac
      361 gttttaaaat taacgccata tccttatcca tctttcttgc ctatgccctt acgccatact
      421 cagaagcggc attggtcaga gacgatgtcg attatcaaat attccgtgac tttgcagaaa
      481 acaaaggcaa attttttgtc ggcgcaaccg atttatcagt gaaaaacaaa caaggtcaaa
      541 acatcggcaa cgcattatcc aacgtaccga tgattgattt tagcgttgca gatgtcaaca
      601 gacgtacatt aaccgtcatc gatccccagt atgccgtcag cgtcaaacat gtaaaaggag
      661 acgaaatctc ttattacggg catcacaatg ggcacttaga tgttagcaat gatgaaaacg
      721 aatatcgttc agttgcacaa aatgactacg aaccaaataa aaattggcat cacggtaatc
      781 aaggtcgtct tgaagactac aacatggcac gccttaataa attcgtaaca gaagtcgcac
      841 ctattgcacc aaccagtgca ggcggcggcg ttgaaaccta taaagataaa aaccgttttt
      901 ctgagtttgt gcgagttgga gcaggtacgc aatttgaata taacagccgc tacaatatga
      961 cagagctttc acgagcttat cgttatgcca ttgcaggtac gccttatcaa gacgtgaatg
     1021 tcacatcgaa tctaaatcaa gaaggcttga ttggcttcgg tgataattca aaacatcatt
     1081 ccccagaaaa actcaaagaa gttttatctc aaaacgcatt gactaactat gctgtgttag
     1141 gcgatagcgg ttcaccatta tttgcttatg ataaacaaga aaaacgctgg gtctttttag
     1201 gtgcttacga ttattgggca ggctatcaaa aaaactcttg gcaagaatgg aatatctata
     1261 aaaaagaatt tgcagatgaa atcaaacaac gcgataacgc cggcaccatc aaaggttatg
     1321 gcgaacatca ttggaaaacc acgggcacaa acagccatat cggttcgaca gcggtaaggc
     1381 ttgccggcaa tgaaaggggt gcaaacaacg gacagaatgt tacctttgaa aacaacggca
     1441 ctttggtatt ggatcaaaac atcaaccaag gcgcgggcgg tctgtttttc aaaggcgatt
     1501 acacagtcaa aggtataaat aatgacatca cttggctagg tgcggggatt gatgttgccg
     1561 acggcaaaaa agtcgtttgg caagtcaaaa atccgaatgg cgacagattg gcgaaaatcg
     1621 gcaaaggcac attagaaata aacggcacag gcgttaacca agggcaatta aaagtcggcg
     1681 acggtacggt tattctgaat caaaaagccg attcaaacca aaaagtccag gctttctccc
     1741 aagtcggcat tgtcagcgga cgcggtacat tggtattaaa tagttcaaat cagattaatc
     1801 ccgataacct atatttcggt ttccgtggcg gtcgtttgga tgccaatggc aatgacttga
     1861 cttttgaaca catccgcaac gtggatgaag gcgcgcgcat tgtcaaccac aacacaggcc
     1921 acacctccac aatcacgcta acgggtaaat ctttaattac caatccgaac agcctttcgg
     1981 tacatagtat acaaaatgac tatgatgaag atgattatag ttattattac cgaccaaggc
     2041 gacccatccc acaaggcaaa gatctttatt acaaaaacta tcgttattac gccctaaaat
     2101 ccggcggcag cgtgaacgca ccgatgcccg aaaacggaca aacggaaaat aacgactgga
     2161 ttttaatggg cagcacacaa gaagaagcca agaaaaacgc gatgaaccac aaaaacaatc
     2221 agcatattag cggttttagc ggtttctttg gcgaagaaaa cggaaaagga cataacggtg
     2281 cattaaacct taatttcaac ggcaaaagcg cgcaaaaccg tttcttgtta acaggtggca
     2341 ctaatttaaa cggaaaaata agcgtaactc aaggcaatgt cttgttatca ggtcgtccaa
     2401 caccacacgc aagagatttt gtgaacaaat cttcagcccg aaaagacgca catttctcca
     2461 aaaacaatga agtcgtattt gaagacgact ggataaaccg cacattcaaa gccacagaaa
     2521 ttgcggttaa tcaatccgca tcattctctt ccggcagaaa tgtatccgac atcaccgcca
     2581 acatcaccgc aacagacaac gccaaagtaa atttgggtta caaaaacggc gatgaggttt
     2641 gcgtgcgctc ggactatacc ggttatgtta cctgcaacac aggcaactta tccgataagg
     2701 ctttaaacag ctttggagcg acacagatta acggcaatgt gaatctgaac cagaatgcgg
     2761 cattggtctt gggcaaggct gcattatggg ggcaaattca agggcaagga aacagccgtg
     2821 tcagcctaaa ccaacatagc aaatggcatt tgactggcga cagccaagta cacaatctgt
     2881 cattggcgga tagccatatt catttgaaca atgcctccga tgcgcaaagt gcaaatcaat
     2941 accacacgct caaaatcaat catttatccg gtaacgggca ttttcattat ctgacgcacc
     3001 tggcgaaaaa tcttggggat aaagtgcttg tgaaggaatc cgcatccggt cattatcagc
     3061 tccatgttca agataaaaca ggcgaaccca atcaggaagg gctgaatctc tttgatgcat
     3121 catccgtaca agaccgctcc cgcctttctg tttccttggc gaataaccat gtagatttag
     3181 gggcattgcg ttatacaatc aaaacagaaa acggtattac ccgattgtac aatccttatg
     3241 ccgagaaccg ccgccgagtc aaaccggccc cgtctcctgc cacaaacacg gcttctcaag
     3301 cacaaacgga cagtgcacaa attgccaagc ctcaaaatat cgtcgtcgca ccgcctagcc
     3361 cgcaggcaaa tcaagccgaa gaagcaaaac gccaacaagc aaaagcggag caagtgaagc
     3421 gtcagcaagc agaggcagaa aaagtcgcac atcaaaaggc tgaagaagcg aaacgtcagc
     3481 aagatgccct tgcccgccaa caagcagagc aagaacgcca acgattggaa gccgaacgcc
     3541 aagctgccga aattgccaaa caaaaagcag aagccgaaga agcgaaacgc cgagccgctg
     3601 aaattgccga gcaaaaagca gctgccgaag aagcaaaacg ccaagctgcc gaacttgcac
     3661 gccaacaaga agaagcacga aaagctgcag agttggctgc taagcaaaaa gcggaaactg
     3721 aacgcaaagc agcagaaatt gccgagcaaa aagcggaagc cgaacgtgaa gccgccgaac
     3781 ttgccaagca aaaggcggaa gaagaaggtc gccaagcagc acaatctcaa cccaaacgtc
     3841 gtaaccgcag ggctatacca ccggaactgt catctgatgc aacaacccga gctttgccta
     3901 ggatagcgag aaattcaaat cctgatgcca gcgattatga agaaatcccc ttggatgcgt
     3961 tggaagatga agatgtatcc gaatcggttg acacatcaga caaacagcct caagacaata
     4021 cggaacttca tgaaaaagtt gagacagtga gtttgcaacc aagagccgct cagccgcgag
     4081 cccaagccgc cgcgcagccg caagcccaag ccgtcgcgca agccgatgca gtcagcacca
     4141 ataccaactc ggctttatct gacgcaatgg caagcacgca atctatcttg ttggatacag
     4201 gtgcttcatt aacacggcac attgcacaaa aatcacgcgc tgatgccgaa aaaaacagcg
     4261 tttggatgtc gaacatcggt tatggccgtg attatgcttc cgcacaatat cgccggttta
     4321 gttcgaaacg cacgcaaaca caaatcggca ttgaccgcag cttgtccgaa aatatgcaga
     4381 taggcggcgt attgacttac tctgacagtc agcatacttt tgatcaggcg agcggcaaaa
     4441 atacttttgt gcaagccaac ctttatggta agtattattt aaatgatgct tggtatgtgg
     4501 ccggcgatat tggtgcaggc agcttgagaa gccggttaca aacacagcaa aaagcaaact
     4561 ttaaccgaac aagcatccaa accggcctta ctttgggcaa tacgctgaaa atcaatcaat
     4621 tcgagattgt ccccagtgcg ggaatccgtt acagccgcct gtcatctgca gattacaagt
     4681 tgggtaacga cagtgttaaa gtaagttcta tgtcagtgaa aacactgacg gccggactgg
     4741 attttgctta tcggtttaaa gtcggcaacc ttaccgtaaa acccttgtta tctgcagctt
     4801 actttgccaa ttatggcaaa ggcggcgtga atgtgggcgg taattccttc gcctataaag
     4861 cagataatca acagcaatat tcagcaggtg ccgcgttact gtaccgtaat gttacattaa
     4921 acgtaaatgg cagtattaca aaaggaaaac aattggaaaa acaaaaatcc ggacaaatta
     4981 aaatacagat tcgtttctaa aatactaaat tcatagcaaa ataaaatgcc gtctgaactc
     5041 aggcttcaga cggcattttt atagtggatt aacaaaaatc aggacaaggc gacgaagccg
     5101 cagacagtac agatagtacg gcaaggcgag gcaacgccgt actggttttt gttaatccac
     5161 tatatcagac ggacgaggtt acagtttaat ccctttgagc ttcgcgacgg tattgatgtc
     5221 tttgtcgcca cgaccggata ggttgaccag aatcacttgg tctttgccca ttttcggcgc
     5281 gtttttcacc gcccaagcaa cggcgtggct ggattcgagc gcggggatga tgccctcgaa
     5341 gcggcagagc aaatcaaagg cttcgagtgc ttcgtcgtct ttagcgacgg tgtattcgac
     5401 gcgcttgatg tcgtgcagat ggctgtgttc cgggccgatg ccggggtaat ccaagcctgc
     5461 ggaaacagag tgtgtaccca agacttgacc gttttcgtcc tgcatcagat agctgcggaa
     5521 accgtgcaat acgccaatcg gtgagcccga agtaatcggc gcggcgtgat cgggggtgtt
     5581 cacgcccaaa ccgccagcct ccacgccgac gaggcgcacg ttttcttcgc cgatatacgg
     5641 gtggaacaaa ccaatggcgt tcgatccgcc gcccacgcag gcaacggcaa cgtcgggctg
     5701 tctgccgatg gcttccaaca tttgttcttt ggcttcattg ccgatcacgc attgaaagtc
     5761 gcgcaccatt tcgggatacg gcgcgggacc ggcggcggta ccgatgatgt agaatgtgtc
     5821 atccacgcgg gcgacccatt cgcgcatggc ttcgttcatc gcgtctttca aagtacggct
     5881 gccgctgtca acgccgacga cattcgcgcc caataatttc atacggaaca cgttgggcat
     5941 ttggcgttgg atgtcgtccg cacccatgta cacgtcgcaa gtcatgccga agcgggcggc
     6001 aacagtggca ctcgccacgc cgtgctgacc cgcgccggtt tcggcgatga cgcgtttttt
     6061 acccatacgt tttgccaaca gtgcctgacc gatggtgttg ttgactttgt gcgcgccggt
     6121 gtggttcaag tcttcgcgct tcaaccagat ttgcgcaccg cccaaatgct cggacaaccg
     6181 cgcggcgtgg taaacggggc tgggcctgcc gacataatgt ttcaaatcgc ggtggaattc
     6241 ttcccaaaac gacgggtcgt ttttcgctgc cttataggca tccgccagct cttgcaaagc
     6301 gggaatcagg gtctcggaca cgaacagccc gccgtgttcg ccgaaaaagc ccttctcgtc
     6361 gggcgcgtgg tagttcttca tcggattctt ccttgtgctt caggttttca aacggcattt
     6421 ccgttttttc aaacatgccg tccgaacggg aaattgtcgg caattataac tgcttttcac
     6481 acaagcccgc catttcgaaa aacgcccgat acaccgccgc ctttttcgcc aaactcaaag
     6541 gataggcgcg cgaagtcgaa ggcaggcgcg tcagcgtcaa atcccgtccg gcaaacggaa
     6601 acggcacggt ttcgcccgtt ttcggcattt tgatgccgcc accctgaata tcgagcagga
     6661 tttccgtcgc cttgccgcct gtcgtacaaa tatggcggca ctcgggtatt tttgccaaca
     6721 ccgccgccaa atcgacggtt tcaactacct ttaaaaactt gtcggacgca ttgccgtgtt
     6781 cccttgccgc cttcaacacg gtcggacagg acgcaatccc ccgttcgtgc aaaaacgcct
     6841 tgattttctc ggcatcaaac gctttttcag acaacctttg gaaatgcgcc gcatcattaa
     6901 aaaacaccag cccgtaaacg cgccacatat cgttttggaa attcggataa tgaaactgca
     6961 tcgcgcgttt gtcttccttg ggcggaaacg tccccatcat catgaccgca gccttcggcg
     7021 gcaacacgga atcgaaggga tgggtttcga tttcaagcag attttcagac acgttcaatc
     7081 ctccaacagg caaaacggca gacagccggt ttttgcacca aaccgccaaa cacggcaagc
     7141 cttcaaacag cattatcacg ccctgccgtt ttcggttaca atgccgacat caaacggata
     7201 ctgtaaacac atttatgttc caacacacag gacgacactt aaagcaccgc cctatgtgcc
     7261 atcctgattt ggaaggagtt acgcccctcc caaatagagt ctgattctac cgccctaaag
     7321 ggcggggttt caaccgaaaa ggaaacacga tgaaagaaca caaagcccgc aagcgtttcg
     7381 ggcagaattt tttgcaggac acgcggatca tcagcgatat tgtcaacgcc gtgcgcccgc
     7441 aggcggatga tgtcgtgatt gaaatcggcc cgggtttggc ggcgattacc gaacctttgg
     7501 cgaaaaagct gaaccgcctg cacgttgtcg aaatcgaccg cgacatcgta tgccgtctga
     7561 aaacgctgcc gtttgcggat aaactggtga ttcacgaagg cgatgtattg cagtttgatt
     7621 tcaacggcat cgcaggcaaa aagaaaatcg tcggcaacct gccctacaac atttccacgc
     7681 cgcttttgtt caagctggcg gaggtggcgg acgatgtcgt cgatatgcac tttatgctgc
     7741 aaaaagaagt ggtcgatcgt atggttgccg cgccgaaaag caacgactac ggccgcttgg
     7801 gcgtgatgct gcaatatttc ttcgatatgg aaatgctgat tgacgtgccg cccgaatcgt
     7861 tcgaccccgc gccgaaagtg gattcggccg tcgtccgcat gattccggtg aaacaccgta
     7921 tcggcaaggc ggacgatttc gagcatttcg ccaaactcgt gaaactcgcc ttccaccaac
     7981 gccgcaaaac catacgcaac aatctgaaag agcttgcagg cgacgatgat ttgcaggcag
     8041 tcggcatcaa tccgcaagac cgcgccgaac atatcgcgcc ggagaaatat gtggcgttga
     8101 gcaattatct ggcgggcaag gccgtctgaa aagggagtaa gccgatgacg aaatacctgc
     8161 ttttacccct gatactgccg cttgcagcct gcctgaccaa tccgaacgac cgtttttttg
     8221 acggcagacg ctacacgctt catcatatgg agctcccgta acgacaacct gcaacggcaa
     8281 atgccgtctg aaagaaaaga catgatcaaa ttcaaaaacg tacacaaaca tttcaaagac
     8341 ctgcacgtca tcaacggcgt gaacttggaa atcaaaaaag gcgaagtggt cgtcgtctgc
     8401 ggaccttcgg gcagcggcaa atccacactc atccgcaccg tcaaccagct tgaaagcatc
     8461 gaaagcggcg agatttgggt ggacggtgtc aacgttgccg acccaaaaac cgatttgaac
     8521 aaaatccgcg aagaagtcgg ctttgtgttt caaagtttca acctctaccc ccacctgacc
     8581 gtattggaaa acatcaccct cgcaccgatg aaggttaaag gtcaaaatgc ggaacaggcg
     8641 gagaaaaaag cgatggaact tttggaacgc gtcggactgg cgcacaaaaa agacgctttt
     8701 ccgtcccaac tttccggcgg ccagcagcag cgcgtggcga ttgcacgcgg tttggcgatg
     8761 gaaccgcgcg taatgttgtt tgacgaaccg acctccgcac tcgaccccga aatggtcggc
     8821 gaagtgttga aagtgatgaa agacttggca gaaagcggca tgaccatgat gtgcgtaacc
     8881 cacgaaatgg ggtttgcccg cgaagttgcc gaccgcgtga ttttcgtcga taaagggcaa
     8941 atcctcgaag acgaaacgcc cgaagcgttt ttcaccaacc cgaaacacga acgcgccaag
     9001 cagttcctgc aacaggtcat gacccactga tgctataccg tctgaaaacc gaacgttcag
     9061 acggcatagc ccgacaccgc aagatgattg agaaagaaaa ccatgtattt tgttgaccgc
     9121 accgccgtcg tcctcaagcc gaccgtccgc tttttggaat ggctcaaaag caccgacgaa
     9181 aatatgcccg acctgaccat agagcagctt cgtgccaact gctccgtctt cctcgttccg
     9241 cagttcgacg aaccggaagc cgtcgtttcc tatttcgacg aacgctaccg ccagattttt
     9301 gaagccgaac tcgccggctg ggacatcgac aaggataaat ggccgcaaga tatggggctg
     9361 aaggcgtttt gggaattttt cgacatcgaa atccacgata tggtgttgga tatggaagaa
     9421 gcggatttga acatcacgcc cgtgttcgac aatatgatgt aacgccgtgc gcgcctttca
     9481 gacagcattg aggccgtccc gcatcctgaa catcctgacc gtatcgctcc actcggcttc
     9541 ccttgccgtc tgcctgacct ggttttacgg ccggatgatg tggttcggtt tggcggcttt
     9601 ggcggcaagt tacgcatact cgctcaagat aacgaatctg aaacaccgcc atgccataac
     9661 cgccattacc atagaccgcg acggacaggc ggaaatcgta tccggcaaag aaaaaacagc
     9721 aaaggcggca acacttgcgg gcagcagtat ggttacgcct tatgccttat tcttacaatg
     9781 ggacaccggc ggcaaaaccg tccgccactg catcatgccc gacatgacgg acaaagaatc
     9841 ctaccgcaag ctgaaagtct gggtactttg gcggcagccg aaaaaaaccg ccgaaaccga
     9901 tacatcagac tgatttcaga cggcatttga aaacgaaaca ctcatgaaaa cactacaaga
     9961 ctggctctcg catttggaaa ccgcccacag cggcggtttg atcgacatgg gtttggagcg
    10021 cacgagcgaa gtgaaaaaac gcatgaagct cgagccgcaa tgccccgtcg tcgttgtcgc
    10081 gggaaccaac ggcaaaggtt cggtctgtgc ctacctgaca cagatttaca aacaggcggg
    10141 ctacaaaacc ggtacgctga ccagcccgca tttattgcgt tacaacgaac gcatcgccat
    10201 caacgccgaa cccgtttcgg acgatacgat tattgcctct ttcgagcgca tcgaagcggc
    10261 gcgcagcgaa atatcgctga cttattttga attcaatacg ttggcggccg tcgacatctt
    10321 catgcgcgaa caggttgacg tgatgatatt ggaagtcggc ttgggcggac gcttggacgc
    10381 ggtcaacgca tttgacggcg attgcgcggt ggttactagt gtagatttgg accatcaggc
    10441 gtttttgggc gatacggtcg agcaggtcgg ctttgaaaaa gcaggcgtgt tccgcagcgg
    10501 caaacccgca atctgcggtc aaaaccccgc gcccgaatcg ttgctttcac acgccgaagc
    10561 cataggcgcg aaactgctga tggtgcagcg cgatttcgag tttcacgcga tggaaaacat
    10621 ccaatggaac taccgcttcc ggccgcaaca ttcagacgac cccgcgcgca accgcaatgc
    10681 cctgcccttc cccgcattgc gcggcacata ccaactttcc aacgccgcct gcacgttgac
    10741 cgtattggaa tgcttgaacg ccaaattgcc ggtggacatc ggcgccatca aacgcggatt
    10801 gctgctggtt gaaaatcccg gacgcttcca agtcctgccc ggccgtccgc tgaccgtttt
    10861 ggatgtcgga cacaatcccc acgctgcccg cgccctgcgc cgcagcctga ttaatttggc
    10921 gtttgcgcaa aaacgcaccg ccgtgttcag catgttgtcc gacaaagaca tagacggcgt
    10981 gttggaaacc gttaaagacc agtttgacga gtggtatatc gcaccgttgg atgtaccgcg
    11041 cggcatgacg ctcgagagcc tgcaaaacaa actgcacgaa cacagcatcg aaaacataca
    11101 aacattcgcc gccgtccgcg aagcataccg ggcggcagcc tcaaaagcgg gcgagaacga
    11161 cagaatcgtc gttttcggct catttcatac cgttgccgat gtcatgtcgg tactttaaaa
    11221 ggaaacttct atgtccgaaa acaaacaaaa cgaagtcctg agcggttacg aacaactcaa
    11281 acggcgcaac cgccgccgcc tcgtaacggc aagttgcctg gttgccgcct cctgcatcct
    11341 gctggcagcc gccctcagtt ccggccctgc cgaacagact gccggcgaaa caagcggcgt
    11401 agaaaacaaa gcggcaggtg cggcacaaac ccctgccttg aaatccgccg ccgacaaacc
    11461 gcaggacttg gcaggcgaag acaagccttc tgccgccgac agcgaaatca gcgagcctga
    11521 aaacgtaggc gcgccgctgg tgctgattaa cgaccgcctc gaagacagca acatcaaagg
    11581 tttggaagca tccgagaaac tgcaacaggc agaaaccgcc aaaaccgcac cgaagcaggc
    11641 aaaacaacgc gctgccgaaa aagtgccggc aactgccgac agtacggata cggtagcggt
    11701 tgaaaaaccg aaacgcactg ccgaaacaaa accgcaaaaa gcggaacgca ctgccaaagc
    11761 caagcccaaa gccaaagaaa ccaaaaccgc cgaaaaagtt gccgacaaac cgaaaactgc
    11821 cgccgaaaaa accaaaccgg atacggcaaa atccgacagc gcggtaaaag aagcgaaaaa
    11881 agccgacaag gctgaaagca gaaaaacagc cgaaaaagac cgttcggacg gcaaaaaaca
    11941 cgaaacggca caaaaaaccg acaaagcgga caagaccaaa accgccgaga aggaaaaatc
    12001 cggtaaaaaa gccgccattc aggcaggtta tgccgaaaaa gaacgcgcct taagcctcca
    12061 gcgcaaaatg aaggcggtgg gtatcgatt
//
LOCUS       DAWHXZ010000039        11823 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000039 DAWHXZ010000000
VERSION     DAWHXZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11823
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.039"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(71..487)
                     /locus_tag="WIE35_000688"
     CDS             complement(71..487)
                     /locus_tag="WIE35_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153638.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDSD"
     gene            complement(484..675)
                     /locus_tag="WIE35_000689"
     CDS             complement(484..675)
                     /locus_tag="WIE35_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153639.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            complement(719..961)
                     /locus_tag="WIE35_000690"
     CDS             complement(719..961)
                     /locus_tag="WIE35_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153640.1"
                     /translation="MDTLKVNPFPNKIKEKCRLKTARMLFQTAFFVVGHQCPAICAAL
                     RLSRYGILVCIFRRNLPYIVLKYYTVVLYGFDFLEK"
     gene            1198..1365
                     /locus_tag="WIE35_000691"
     CDS             1198..1365
                     /locus_tag="WIE35_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153641.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            1638..1823
                     /locus_tag="WIE35_000692"
     CDS             1638..1823
                     /locus_tag="WIE35_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153642.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            2024..2224
                     /locus_tag="WIE35_000693"
     CDS             2024..2224
                     /locus_tag="WIE35_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153643.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            2356..2964
                     /locus_tag="WIE35_000694"
     CDS             2356..2964
                     /locus_tag="WIE35_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HEZ4153644.1"
                     /translation="MKDNFTMNTQLIPTVSGQLDNQTQALVDAHDLHKFLGVETPFSK
                     WIQRRIEEYGFTQALDFIGVDKIVRTEAGFFGQRDKTVQGYYLSLDMAKELCMVERND
                     KGRQARRYFIEMEKQAKALPDAVLYRIDALEDAYFQAAPEMLALLRYRSMGLNLTEIG
                     KLLDMNPGAVSYRLKKLNDLGFLEYVPKPKMVVQQQSLGLEG"
     gene            2968..3183
                     /locus_tag="WIE35_000695"
     CDS             2968..3183
                     /locus_tag="WIE35_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153645.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLEMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(3344..3862)
                     /locus_tag="WIE35_000696"
     CDS             complement(3344..3862)
                     /locus_tag="WIE35_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ4153646.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAQAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(3972..4139)
                     /locus_tag="WIE35_000697"
     CDS             complement(3972..4139)
                     /locus_tag="WIE35_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153647.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            complement(4132..4305)
                     /locus_tag="WIE35_000698"
     CDS             complement(4132..4305)
                     /locus_tag="WIE35_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153648.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATKFMVKRNREQEVAEYQAQFEDKD
                     GKGRLKTEGNKDE"
     gene            complement(4414..4680)
                     /locus_tag="WIE35_000699"
     CDS             complement(4414..4680)
                     /locus_tag="WIE35_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153649.1"
                     /translation="MQVLKKVDWKMFVARSFWRWVPVGLTVGVWCFVAGMALHSCAKE
                     PEPVAKEPTKVEAMERQADLEVKVWEGQYESMSVEEKMKGIVYE"
     gene            complement(4770..5684)
                     /locus_tag="WIE35_000700"
     CDS             complement(4770..5684)
                     /locus_tag="WIE35_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEZ4153650.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIDTSPASISMYLNGT
                     YADKGGNYETIETKIEAFLEMEDSKARREELVLGFVSTKTTRRIAEVMRDAHEGGETV
                     VIYGQAGLGKTQSVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSVALNLGESLPDDELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKMQAGIVKKYSSLIIR"
     gene            complement(5720..7696)
                     /locus_tag="WIE35_000701"
     CDS             complement(5720..7696)
                     /locus_tag="WIE35_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ4153651.1"
                     /translation="MKYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPAEIRAAIMKRQSDELAEKMPKMLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     NARCAVVAAVLGIKYQYGCSAKAAVAQFLGLLAESKLDAVTLGNLEKANDRSRTAKVG
                     ERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIVGNPQGRGI
                     IERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPSWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYTPKAYREMRLEQDGIAPDMLSA
                     QELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASVVNVFDMD
                     GKFITKAQADGNTREAFPTARIDQLAENRRKGKIKRAENAIKLANAEVNPALEQAAAW
                     DELGHLGGNVIEAEYAVLPKTGTDDFVLFETDRS"
     gene            complement(7698..7949)
                     /locus_tag="WIE35_000702"
     CDS             complement(7698..7949)
                     /locus_tag="WIE35_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4153652.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            7993..8892
                     /locus_tag="WIE35_000703"
     CDS             7993..8892
                     /locus_tag="WIE35_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ4153653.1"
                     /translation="MPALTVTWMIYTKYLVYQIYLVIFCDVFMERIDLALNLVEERSR
                     LGYSRKNFAEQTGCSAESLRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNA
                     VSDDLNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGK
                     EHITLEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDK
                     AETYLRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFSVES
                     LTELSDEDLQKTYAAVSTKKRKK"
     gene            9247..9864
                     /locus_tag="WIE35_000704"
     CDS             9247..9864
                     /locus_tag="WIE35_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ4153654.1"
                     /translation="MVWPEYSPQENVALFRHNGEILLSGINIKLKNGNSLLIRGPSGC
                     GKTSLLRALAGLWPFGSSGKVSHPPHQDILFLPQRPYTAQGSLRDAICYPAIDKQHPE
                     LIEAMNTCRLGYLVDKLDKTDDWQHKLSPGELQRVAFVRALLSQPKVILLDEATAALD
                     EPTEAMLYRALKQKLPDSIIISIGHRSTLNAFHNMHLDVGNVACG"
     gene            9968..10636
                     /gene="hda"
                     /locus_tag="WIE35_000705"
     CDS             9968..10636
                     /gene="hda"
                     /locus_tag="WIE35_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ4153655.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLQHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            10633..11301
                     /locus_tag="WIE35_000706"
     CDS             10633..11301
                     /locus_tag="WIE35_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ4153656.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAKYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLQSYGKTYFYSDSRNDLPLLRLVSEPVAANPDAELEKEAKEKGWPV
                     LNFK"
BASE COUNT         3157 a         3152 c         2439 g         3075 t
ORIGIN      
        1 aaaaatgccg tctgaaaacg agctttgcga gtttcgccaa aacgtttcag acgacctttt
       61 ttactacaat ctaatcactg tctttttcgc gccgttgctt ttccaaggtt tgcgctttac
      121 ctctgttgat tcctgcaaaa tatcccaaag ctaccgcccc gccgattttg acaatttcaa
      181 tagcgacgct gtccttatcg cgccataacg cagtaatcag cacggcggca atcagaaaag
      241 aaatagcaat cacggcattc agacggcgtt tgtgtacctc tttgaaaatc tcaccatgtt
      301 gctgttcacc ctgtttttgc gcttcgatgg tagccagtgc aatgcgttcg tgagattgga
      361 tttcgtcctt acggctttcc aactccttct gtttgactgc aatatcctgt ttttgaagtt
      421 ctaaaaactc ctgaagcatt ttctgctgct catcggccgg cagattctcg gatttttccc
      481 cgctcataaa tcaaccgcca ctaaggtttt gtaacggact ttgaatttgc cgtaaccccg
      541 agcccctatt ttcggcggca caaattccac ggacgcaata ttgcccgtct tacggtcttt
      601 acggacttct tccaaataat cggcagcatt gagcagccgt tcgcggactg ccgtatattc
      661 tttgacggta ttcatttttc aacctcgccc aaatagtgca aatattagca tatgggattt
      721 atttttcaag aaaatcaaat ccatataaaa caaccgtata atattttaat actatatatg
      781 gtaaatttct tctaaaaata cacactaaaa taccatatct tgataaccgc aacgccgcac
      841 aaatggcagg gcattgatgc ccgacaacaa aaaatgccgt ctgaaacagc attcgagctg
      901 ttttcagacg gcatttttct ttgattttgt tggggaaagg gttgactttt aacgtgtcca
      961 cgtttataat tcaattcatc ggcgtagtga gggccgatac gggaaaagcc ccaccgaagc
     1021 ggggctcaac tgggagaaag aagatgaata aacgcactat tcaaattctc atcttggtcg
     1081 cagttttcat gttggcaagt gctaatgcct actaaagact gaccagctgg gggcggtcgc
     1141 cgctgtcccc agtcccaatc ttacaaaata cggtttaaaa aatcaaggag tttgcctatg
     1201 gttgatgaaa aactggcaga aaaccgaaag cgttacgagc agaaacgggt gattaagaaa
     1261 gtctcgttca atgccgaaac ggaaaaagaa cttttggaat atgcccaaaa tatcgatttt
     1321 tcccaatggg ttaaatcaat catcaaagaa aaaatcaaaa aatagcttga ctaaattcta
     1381 gaaatactag ataatacgcc tatctgataa agcaaagccc cgaagcggca acttcggggg
     1441 cttatcggat agaaaggagg cttgtggaaa tgacaaaact ggcgaaagtt gtttttttca
     1501 tcattctgct tttaagctgt agtccggctt actaagcaag ttgattaaac acaaggggtg
     1561 gcagaacacc gcgccccttt cgccaacctt tctgcaagcc attttactac aacgatttta
     1621 aaaatcaagg attgcctatg cctgatgaaa aaacgctaaa agcaaaacaa gctgaatatc
     1681 agcgaaagta tgaccaaaag cggctgatga aaacagtttc tttccatttg gaaagagaga
     1741 aagagttggt ggattttatg caaaaagaaa ttcccgactt ttcaaactgg gttaaatcag
     1801 tcatgaagga aaaaatgaaa taagaaaatt ccgccgatgg gaaaacatcg gcggaacagg
     1861 tggaaagcag agcagcaact ctctttatgt agggcatgaa aaaaggccgt ctgaaacagg
     1921 ttttaaaccc catttcagac ggctttttgt tgtaatacat cttgattaaa atggttttta
     1981 gtattaccat aatcgtgttt taaaacctat ttaaaggaag attatggact cgattaaaaa
     2041 ggctcaagaa aaatacgcac tcaagcgagt agtgaaaaca gtatcgttca attccgaaaa
     2101 agaacttgat ttaattaaat tttctgaaaa tttagatttt tcaaactggg taaaatcgaa
     2161 aattagagga gatatggaat cggaaaaatt caaaaaaaaa gcaaaaaaga atattgacaa
     2221 atagaattac cataatttaa aatttccata tcttgtgcaa cacaagatat ggaaatcccc
     2281 gataagtgac agcttatcgg ggatagtgga aaagcaacgt tagagcgttc ttttccttgt
     2341 catctcaacc ttgaaatgaa agataatttt accatgaata cccaactcat cccaactgtt
     2401 tcaggtcaat tggacaatca aacacaagca ctggttgatg ctcatgacct gcataagttc
     2461 ttaggtgtgg aaacaccatt ttctaaatgg attcagcgtc gcattgaaga atacggattt
     2521 acacaagccc ttgattttat cggcgtggac aaaattgtcc gcaccgaagc aggtttcttt
     2581 ggacagcgtg ataaaactgt tcaggggtat tatttatccc tcgacatggc aaaagagctg
     2641 tgcatggtgg agcgcaacga caaaggacgc caagcccgcc gatattttat cgaaatggag
     2701 aaacaggcaa aagccctgcc tgatgccgtg ctataccgca tcgatgcatt ggaagacgcc
     2761 tatttccaag ccgcgcccga aatgctcgcg ctgctgcgct accgcagtat gggcttgaac
     2821 ctgaccgaaa taggcaaact cttggacatg aaccccggcg cggtttccta ccgtctcaaa
     2881 aaactcaacg atttgggttt cttggaatat gtccccaagc ccaaaatggt ggtgcaacaa
     2941 cagtctttgg gattggaggg ttaagccatg aataccacct ataccctgac tttcgaccaa
     3001 gacagcctca aagccctgaa tctgctggtg ttcacactca accatctgaa agttttggag
     3061 atggacatgg aaggaatcga agacggtttg tctgccgtga ttgaaaccgc ctccgagaaa
     3121 gccgacaagc tgtccgccgc tttctacaac gccgtgtacg agcaggaaga gaaagcagcc
     3181 tgaatctagg gcatgaaaaa aggccgtctg aaacaggttt taaaccccgt ttcagacgac
     3241 ctttaaatca tgggttaaag cctatttgaa ccggctaggc aagctcctgc tccgtaggct
     3301 caatcacgtt tcaacacact gaattgaaat caggctgttg attttataga atttgttcgc
     3361 tcggctcaat cacaaaatcc tccacgccgc tcacaatctt aatccccggc acttggccgt
     3421 ctgaaaaacg ctctttttga ttcaggatgg cgtctttgtc gatttccttt ttagtgcgga
     3481 caaactcggc aaaggcggat ttttccgaga gccacgccaa gacggcggcc acgcccgtta
     3541 ccttgacgga tggcggacgg atgcgccatt taatcaggcc ggtagtaaag tccacggttt
     3601 tggttttacc gttttccgtc agctcgtcct tatgtgcctc gcagtatgcg gccacacgtt
     3661 cggtcaggct catgatttcg gcacacatcg gcgcggcttg ggcggcatat tcttcttcga
     3721 tggccgcttt tttgtctccg gcttcggttt ccaggcgttt gacttcgcgc tgcaagtcgc
     3781 cgatttggcg gataaatgca gtaacttccg ttttgtcttg tgccgcttcg atggcgggct
     3841 gtttgattcg ggttttagcc attttctctt cctttcaggt tgggtttaac atttcggctt
     3901 actcaagcct tgttcgcgtt cgtgcttggc aagtgcttca actttcgcgc ggtttctcaa
     3961 aaacttaaac atcacacttc ctcccataaa gttatcgcct gagccaaagt ttccgcctcg
     4021 gcagtcttcc acatcccgtc cggcgcacgc gctgcaatca caaagccttc gccgtccttc
     4081 ttcatgacca tcagctcgcc acggtcttcg agccattcga ttaaatcttt ttcattcatc
     4141 tttgtttcct tccgttttca gacgaccttt gccgtcctta tcttcaaact gcgcctgata
     4201 ttcggcgact tcctgctcgc ggtttcgctt caccataaac ttcgtcgcac gcctgcggtg
     4261 ttgtccccaa gcctgccaat ccgtattacg tcgtccgaaa ctcatttcac acatcctttc
     4321 acaatcgcct tatcgccata tttcgcgcgg atttccttta ccgcccgtgc caaagcctct
     4381 tttttcgccg cagggctcaa taaaattggc ttatcactca taaacaatcc ctttcatttt
     4441 ttcctcgacg ctcattgact cgtattgccc ttcccatacc ttgacctcca aatccgcctg
     4501 tctttccatc gcctcaacct tcgtcggctc tttcgcaacc ggttcgggtt ctttcgcgca
     4561 ggaatgcaac gccattcccg ccacaaaaca ccacacgccc accgtcaaac caaccggcac
     4621 ccaacgccaa aaagagcgcg ccacaaacat cttccaatca actttcttca aaacttgcat
     4681 tttgcgtttt ccttttaaaa caacaactta ttaaaatcat agggtaaaaa aatatatagc
     4741 cctgtcagag acttaccgtt tcagacggcc tatcggataa tcagggagct gtatttctta
     4801 acgataccgg cctgcatctt gataccgttc ttgttcgccg tgcgtaccgc gccgcgcatc
     4861 aatttactca tccggcgcgt attgccgtta ctatgtttaa ccagttccaa gagcgtttct
     4921 tcgtccgcat caggcaaagc cgctttcgca atctcaaaga gttcgtcatc cggcaaagat
     4981 tcgcccaaat tcagcgcaac agacacgcgg ctataaagtt gcaccagctc gccatgctta
     5041 ccgcgcaaat tcgcaaccag tcggggcata ccgctcaaca ccaaaccgca gcccgtttca
     5101 tcatgcagtc ggcggataat ctcaagggcg cgcaagggca ggttttccgc ttcatcgacc
     5161 acaatcagac ggcccgaatc gcgcaggcgg tcagatacag actcaaacaa atcattcagg
     5221 ctgcccatcg ccgatacctt cgccgccgtc gccaacttgc gcatcagtac caaagccgta
     5281 aagctcggat tagcctcaat caagatagcc gcagggtttt tctcgcagta gtttttgacc
     5341 gactgcgtct tgcccaaacc cgcctgaccg tagatcacca ccgtttcgcc gccttcgtgc
     5401 gcatcgcgca tcacttccgc gattcggcgt gtcgtcttag tcgatacaaa tcccaacacc
     5461 agctcttcac gtcgcgcctt actgtcctcc atctccaaaa acgcctcgat tttcgtctcg
     5521 atggtttcat aattgccgcc tttatcggca taagtgccgt tcagatacat actgatggag
     5581 gccggcgaag tatcgatacc gcgtgccagt tgggtttggt tcattcccga tttttcttta
     5641 aattcaacca gtttttgttg caatgcttga ttaatttgtt tcattttttt agtcctttta
     5701 aaagaggttt aaaaccgttt taacttctat ccgtctcaaa tagcacaaaa tcgtccgtgc
     5761 ccgttttcgg caataccgca tactccgcct cgatgacgtt tccgcccaaa tgtcccagct
     5821 cgtcccaagc tgccgcctgt tccagtgccg gattgacttc cgcatttgcg agcttgattg
     5881 cattttccgc ccgcttgatt ttgccttttc ggcggttttc cgccagttgg tcgatacgcg
     5941 ccgtcgggaa agcctcgcgg gtattgccgt cggcctgcgc cttagtgata aacttgccgt
     6001 ccatatcaaa cacattgacc accgacgcat cgctcaaatc gtagctgacc cgtacctcgt
     6061 ccttgtgata ctccgccagc tcggttgaga aataagagtt gttgaacaaa tccagccaac
     6121 cgcgctgtac ctttcgcacc tcttgcggca taaacatcgt cgccagctct tgcgccgaca
     6181 acatatccgg cgcgataccg tcctgttcca gcctcatttc ccgataagcc ttcggcgtat
     6241 aatgcccgcc gtcaggatgt cggggcagct cgccgtgcgg gcggttgttg tattcgtcga
     6301 tacacttgac cacatccgcg ataaaacgcg accagctcgg cagttttttc aaatatttct
     6361 gttgttcctc cgttaaatcc ttgccttttt ccaaagcatt aaacgcactt tccatcttgc
     6421 ggtacatcag gttcttcgta ctgctgtcca ttcctgcgcc cgcaaacgtc tcatactggc
     6481 gcgccatctc aatcagattg tctttccacc atcgctcaat gatgccgcgt ccttgcgggt
     6541 tgcccacgat acccgtttca tgccggatac ccaatcggga cgtaatacct gtgatttcat
     6601 ggtctatcgt cttgccggtt tggccgccgc cgttatccga gtaatagata atcggcaaac
     6661 caaaatgctt gaccccgata cgcagagcgt ccgataccgc cacacaactt tcagcaagag
     6721 agaccgaaaa cccgaccaca aaccgcgtac aaccatcaat aatcaccgtc acttccggct
     6781 taaacggcct gccgtgtaca gggtgcgcca ccttcgcctt aaagctgtgg ccgtcgccga
     6841 tccaaacatc gttcggcttc aaagcccccc aatcacgttt cacataaggc agcagcgatt
     6901 tataagccgc ccccgttttc ctgccgcgct cctgcataat cagcggcagc ttgtcccaaa
     6961 cgcgacgcac catactcaaa ttaggcacat cattgaccgg catattttcc gcttcagccc
     7021 actgcacaaa tcggcggtaa ctgtgcgcca gcttcggagc tgaagggatg ttgtgaaact
     7081 gcataaacat cggcaaccaa ccgtagctct caatcggctt gaccgccttc gtcgtcttcg
     7141 gagccaaagc aaccaaccgc tccgtcgcgt tttccgcttt caaataagca gaaatccagc
     7201 cgtctaaagt acgttcgcca acctttgccg tccggctgcg gtcattggcc ttttccaagt
     7261 tcccaagcgt gaccgcgtcc aatttacttt ctgccagcaa gcccaaaaac tgagccaccg
     7321 cagcctttgc agagcaaccg tattggtatt taatccccaa taccgccgcc accaccgcac
     7381 atcgcgcatt cgccaccgac cgttgtttct cgttcaacag cttggccgct tcagccagtg
     7441 cctgagccga catcgccgtc cccggtctga cttggggcag cattttcggc atcttctccg
     7501 ccagctcgtc cgactgccgt ttcatgatgg ctgctcggat ttcggcaggg agggcatatt
     7561 caacaccacc acccttacct tgacgccttc tcgtttgcca accatccctt tgcgcttttt
     7621 ttctcagccc tattactgtt cttggcatat ttggcaaccc aaaattaagc aggcttttga
     7681 ggtccatata tttcatttca acccactccg agcatctctt tcaattcagc tatctttttg
     7741 tctcgacaat ctgttttctt gtaatgcgga aaaacttcaa ctaaatcttt ttgaatcact
     7801 ttcgcgattg cttccgcaat tcttttgctt cctcgtccat ttcttatgac ctcagaaaca
     7861 gattggttag ttacccctaa agccgaagcc aacattcttg ccgatatatt tttttcttct
     7921 aaggatttat agatttcgtt cgcttgcata atgcctccat gatagaatcc attacttacc
     7981 ttacaggctt taatgcctgc cttaactgtt acttggatga tatacacaaa atatttggta
     8041 taccaaatat atttggtaat attttgcgat gtttttatgg aacgcattga tttagcattg
     8101 aatttagttg aagagagaag ccgacttgga tactctcgaa agaattttgc tgagcaaact
     8161 ggatgcagtg cagaaagttt gagactgtat gaaagcgggc aggtcaacat tcctgcggac
     8221 tttttacttg ctgctgtcca attaggggtg gatattcaat ttgtctttac aggcattcat
     8281 tcggcaaatt taaatgctgt ttcagacgac cttaataaaa aattggacga cgctattggc
     8341 aaatcaaacg ttgagaatca gattaacggc aacgtcagta acgctgttat cgcctcatct
     8401 ggttcggttg ttcatcaaat taatacccaa aatcacgtca tcagaacccg tgtcgattca
     8461 aaacccggca aggaacatat aacgcttgag caagcttcaa agctgcaaca acttgttaaa
     8521 caagttgccg cagcagaaga gattgccaaa cggtcgccaa aatcaatccg tgcaatttgg
     8581 gctagtctga atgctcactg taaagttcca agttacaaac taatcgcatt gagcgattac
     8641 gacaaagcag aaacttatct cagaaaatgg ctgggtaggt tgagcaatac agcaacatca
     8701 aagaacaacg atcccgactg gcgaaaaaag aaatacgctt atatcaaatt aaatgtaaaa
     8761 cagcttgagc ttgaagattg gttgaaaagt tacctcgaaa aaaatttctc agtagaaagc
     8821 ctgactgaat taagtgatga agatttgcaa aaaacatatg cagcagtatc gactaaaaaa
     8881 cggaaaaaat aggcttaaac atgaaactcc gcctttaaat tcaagtttca tgtttagctt
     8941 atggtcttca gatgctttgt ttttttagat gtatgatatt aaaagatttt ttcaaacatg
     9001 gaactattat acaagttcca tgttcagttt ctagttaaag ccttttataa acattctaat
     9061 caaccacgcc gaaaccgagc cgcgcgacgg cctatatgtc ttgcgtatag gcaatgacct
     9121 ctttgtcaaa cgtgtgcaac gtataccggg caaactgctt gtaacgtctg aaaatccacg
     9181 ttacgcaccg tttgaaatag atttgagtaa tacccaagac gacatcgcca tcataggccg
     9241 tgttgagtgg tatggccgga gtattcccca caggaaaacg tcgctctgtt ccgccacaac
     9301 ggcgaaatcc tgttgagcgg catcaacatc aaactcaaaa acggcaactc cctactgata
     9361 cgcggcccga gcggttgcgg caaaacttcg ttgctgcgcg cgctggcggg gctttggccg
     9421 ttcggcagca gcggcaaagt cagccatccg ccgcatcaag acatcctctt cctgccgcaa
     9481 cgtccgtaca cggcacaagg cagtctgcgc gatgcgattt gttaccctgc catcgacaaa
     9541 caacatccgg aactgatcga agccatgaat acctgccgat taggttatct ggttgataaa
     9601 ttagataaaa ccgatgactg gcaacacaaa ctctccccag gcgaactgca acgtgttgcc
     9661 ttcgtccgcg ccctactctc gcagcccaaa gtcatcttgc tcgatgaagc taccgccgct
     9721 ttggacgaac cgaccgaagc catgctctac cgcgccttaa agcaaaaact gcccgacagc
     9781 atcatcatca gtatcggcca ccgcagcacg ctcaacgcgt tccacaatat gcacctcgat
     9841 gtcggcaacg ttgcctgcgg ctgaacgatg ccgtctgaag ccgttttcag acggcatcgt
     9901 caaattggaa aggcgcgcga aaacaacgta caataaacct atctgccaac ctctgaaatg
     9961 ctgcaccgtg aaccagctta tttttgattt tgccgcacac gactatccaa gtttcgacaa
    10021 attcctcggc acggaaaatg cggagctggt ctatgtcctc caacacaaac acggacagtt
    10081 tatctatgtc tggggtgaag aaggcgcggg aaaaagccac cttttgcagg catgggtcgc
    10141 acaggcactc gaagccggca aaaacgccgc ctatatcgat gccgcctcca tgccgctgac
    10201 cgatgccgcg ttcgaggcgg aataccttgc cgtcgatcaa gtcgaaaaac tgggcaacga
    10261 agaacaagcc ctgctttttt ccatcttcaa ccgcttccgc aacagcggca aaggcttttt
    10321 gctgctcggt tcggaataca cgccccagca gcttgtcatc cgcgaagatt tgcggacgcg
    10381 tatggcatac tgcctcgttt acgaagttaa gcccctaacc gaccaagaaa aaatcgacgc
    10441 gctcgccagt atggcggcgg cacgccaagt aaccgttgat tccgaaattt tcgaatacct
    10501 gctcaaacac tggcggcgcg atatggacag cctgatgatg atgctcgaca cgctggacaa
    10561 ctacgccgtc acgatgggca aacgcatcac cctgccgctt ttgcgccagc ttttgaaaca
    10621 acaggaaacc caatgaaaaa cctcgccatc ttcgacctcg acaacaccct catcaacacc
    10681 gattccgacc acgcctggcc gcaatacctc atcaaaaaag gcttggttga cgctgccgaa
    10741 accgaagcgc aaaacgaaaa attctaccgc gactaccaaa acggctgcct cgacatcgac
    10801 gcattcctca aattccacct cgccccgctc gcccgctaca gcaaagaaga gctggcggaa
    10861 tttcaccgcg aatttatggc gaaatacatc atcccccaca tctcgcctat gcagcgtatg
    10921 ctggtgcaga gccaccaaat ggcaggcgac gaaaccctcg tcatctcctc aaccaacgag
    10981 ttcatcatca cccccgtctg ccaccttttc ggcatcacca acatcatcgg cacacaactc
    11041 gaaaccggtt ctgacggccg ctacaccggc aattacatcg gcacgcccag cctcaaagaa
    11101 ggcaaaatta cccgcctgaa ccaatggctt gccgaacgcg gcgaaacgct ccaaagctac
    11161 ggcaaaacct atttttacag cgactccaga aacgacctgc cgctgctgcg cctcgtcagc
    11221 gaacccgtcg ccgccaaccc cgatgccgag ctggaaaaag aagccaaaga aaaaggctgg
    11281 ccggttttga atttcaaatg acgcaatgcc gtctgaagcc cgattcggcg ttcagacggc
    11341 attggggctg aaatcccaaa aactcaaagc cacaggaatt tatccggaaa aacaaaaaac
    11401 acaggaattc atcgggaaaa acaacaatct ttccaccgtc attcccacga aagtgggaat
    11461 cttgatccgt ccgcacggaa acttatattt cgtcatttgc acccgacata attcaatact
    11521 ttattttgca ataaattaac ggatgctgac aaattagatt ggattaatta ccctattatc
    11581 catattgggt tatcgggttg ctaattgagc agagcgtagc aaaccataaa gtgagtgttt
    11641 ccacccactc tgcatttact atgaagtggt tattaaatat gataatgaga cctattaaat
    11701 ttagtatggt aaatacgtta ttatttattg ttatatgtag ttcatttttt gatctgctcg
    11761 ttcaattatg tacaatttta tttcatagcc aaaaaatata ctttattaca ttatttttat
    11821 tat
//
LOCUS       DAWHXZ010000040        11745 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000040 DAWHXZ010000000
VERSION     DAWHXZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11745
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.040"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(105..1892)
                     /gene="lpdA"
                     /locus_tag="WIE35_000707"
     CDS             complement(105..1892)
                     /gene="lpdA"
                     /locus_tag="WIE35_000707"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ4153657.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAPAAAPAQEAPKAA
                     APAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNV
                     GCIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAK
                     SRKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAATTGEKKIVAFKNCIIAAGSRVTKLP
                     FIPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQ
                     GADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLV
                     AAGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHE
                     GHVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASG
                     RAIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIH
                     PHPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1969..3552)
                     /gene="aceF"
                     /locus_tag="WIE35_000708"
     CDS             complement(1969..3552)
                     /gene="aceF"
                     /locus_tag="WIE35_000708"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ4153658.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAASAAAAPVAAGGATVQV
                     AVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAVFLKVG
                     DKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPSAPAAAKID
                     EAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAKPAA
                     AGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEEAD
                     MTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFN
                     IGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTISSL
                     GGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDGAAG
                     MRFTVFLAKLLKDFRRITL"
     gene            complement(3702..6365)
                     /gene="aceE"
                     /locus_tag="WIE35_000709"
     CDS             complement(3702..6365)
                     /gene="aceE"
                     /locus_tag="WIE35_000709"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ4153659.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSDFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVVT
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSKLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALSA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7059..8771
                     /locus_tag="WIE35_000710"
     CDS             7059..8771
                     /locus_tag="WIE35_000710"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ4153660.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDSYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVRSPLTMASPAAIVEQFGANGG
                     SLGPVGFAGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVVRVTA
                     LLNG"
     gene            8834..9490
                     /locus_tag="WIE35_000711"
     CDS             8834..9490
                     /locus_tag="WIE35_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ4153661.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9500..9955)
                     /gene="ruvX"
                     /locus_tag="WIE35_000712"
     CDS             complement(9500..9955)
                     /gene="ruvX"
                     /locus_tag="WIE35_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ4153662.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9948..10496)
                     /locus_tag="WIE35_000713"
     CDS             complement(9948..10496)
                     /locus_tag="WIE35_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ4153663.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10498..11058)
                     /locus_tag="WIE35_000714"
     CDS             complement(10498..11058)
                     /locus_tag="WIE35_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ4153664.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11386..>11745
                     /locus_tag="WIE35_000715"
     CDS             11386..>11745
                     /locus_tag="WIE35_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153665.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKSVSGS"
BASE COUNT         2618 a         2989 c         3272 g         2866 t
ORIGIN      
        1 gtcggagaag atattttaat ccgctgtaaa atagaaaatg ccgtctgaac atttcagacg
       61 gcatttgctc aaataaacct tatcggctgt ttattcagtc ggatttattt tttcttttgc
      121 ggaggcaggt cggtacaagt acccaatgcc acttccgccg ccataccgat ggattcgccc
      181 aaggtcgggt gcgggtggat ggttttgccg atgtctgccg cgtcgcagcc catttcgatg
      241 gcaaggcaga cttcgccgat catgtcgcca ccgttcggac cgacaatgcc gccgccgatg
      301 atgcggccgg tttcggcatc aaaaatcagc ttggtaaagc cgttgtcgca accgttggca
      361 atcgcacggc cggaagccgc ccacgggaag ttggctttgg tgattttgcg gccggatgct
      421 ttggcggaca actcggtttc gcccacccac gccacttcgg gggaagtgta ggcaacgccc
      481 ggaatcacgc gcgcgtcgaa gtaggctttg tggccggcgc agttttcagc agcaacgtgg
      541 ccttcgtgaa cggctttgtg tgccaacatt ggttgaccga cgatgtcgcc gatggcgtag
      601 atgtgcggca cattggtacg catttgtttg tccacttcga tgaagccgcg atcggtaacg
      661 gcaacgcctg ctttttccgc gctgatgagt ttgccgttgg gcgcgcggcc ggcggcaacc
      721 aatacggcat cgtaacgttg cggctcttta ggcgcatttg caccttcaaa ggtaacgtaa
      781 acgccgtctt ctttcggctc gactgcaacg gttttggtgt tgaccataat gttgtcaaaa
      841 cggtattcgt tttgtttttg ccatactttc accaagtcgc ggtctgcgcc ttgcatcagg
      901 ccgtccatca tttcaaccac atccaaacgc gaacccagcg tgctgtaaac cgtacccatc
      961 tcgaggccga taatgccgcc gccgataatc agcagtttgc ccggtacttc tttcagagcc
     1021 aatgcgccgc tggaatcgat gatgcgcgga tcttcaggaa tgaaaggcag tttggttacg
     1081 cggctgcctg ctgcaatgat acagtttttg aaggcaacga tttttttctc gccggtagtg
     1141 gctgcctgtt cgtacgcgtc gccggcagtc agcgacactt ccaagtggtg cgggtctaag
     1201 aattgcccgt cgccttggat aacgtccact ttacggcttt tcgccatacc tgccaaaccg
     1261 cccgtgaggc gggaaactac gccgtctttg taggcgcgaa gcatatcgat gtcgagttcc
     1321 ggctcggggt atttgatacc gttggcagcc aagtggcgca cttcgtcgat aacggcggca
     1381 ttgtgcaaca aggctttgga agggatacag ccgacgttca ggcaaacgcc gcccaaagtt
     1441 ttgtaacgtt cgacgatggc gactttcaag ccttcatcgg cagcggcaaa tgcagcggag
     1501 taaccgccgg gaccgccacc caatacgacc acgtcgtact cggcatcggc agaaccgccg
     1561 aattgcgcgg cttgcggagc aggagcagca gctttagggg cttcttgcgc cggggcggca
     1621 gcaggtgctt cagctttggg agcggctgcc gtgccttcag cttcaacgac gacaatcaag
     1681 ccgccttcgg agattttgtc gccgactttg actttcactt ctttgaccac gcccgcaact
     1741 tcggcaggca cgtccatcgt cgctttgtcg gtttccaggg taatcagggt atcgtcaacg
     1801 gcgatggtgt ctcctacgtt cacttcaact gcgataatat ctacattttc gtgtccgcca
     1861 atgtcgggca ctttcaattc aactaagctc atgtctcatc tttctgaatg atgttcaaac
     1921 ttccttttca gacggcacag gccgctatgc cgtctgaagc cgatcgggtt acagggtaat
     1981 gcggcggaag tctttcaaca gcttcgccaa gaatacggta aagcgcatac cggccgcacc
     2041 gtcgatgacg cggtggtcga aagacaggct caacgggcac atcaggcgtg gggcaaattc
     2101 tttgccgttc caaaccggtt tgatttggga tttgcacacg cccaagatgg cgacttcggg
     2161 agcgttcaca atcggcgtga aacctgtgcc gccgataccg cccaagctgg aaatggtaaa
     2221 gcacgcgcct tgcatttctt gcggtttgag cttgccttcg cgggcttttt tagacaattc
     2281 ggtcagctct tggctgattt gtttcaagcc tttttgatcc acgtctttga tgacgggaac
     2341 aaccaagccg ttcggcgtat cggctgcgaa accgatgttg aagtagtttt tcagcaccag
     2401 gttgtcgccg tccagagaag cgttgaattc ggggaaggct ttcagcgcgg aaacggaggc
     2461 tttgatgatg aacgctagag gagacagttt cacgccttcg cgttcccatt ctttgttcag
     2521 ttgtttgcgg aattcttcca gctcggtcat atctgcttct tcgtgtacgg taacgtgggg
     2581 aatcaccacc cagttgcgag acaggttttg accggagatt ttcttaatgc gggacaattc
     2641 tttaacttcg acattgccga atttggagaa gtccacttta ggccacggca gcaagtccag
     2701 accgctgccc aaagatgcgc cggctgcggc aggttttgcc gcgccaccct gcattacgga
     2761 tttcacaaag gctttgatgt cgtcgcccac gatgcggcct ttcaagccgg tacctttgac
     2821 ttggcccaaa tccacgccca attcgcgcgc cagtttgcgt gcggaaggac cggcgtgtgc
     2881 tttggcgaaa gcggcctcgt cgattttggc agcggcaggt gcagaaggtg caggcgcggc
     2941 ggcgggtgcg gcggcagcag tcggaggcgg agcggcagcc ggtgcggcag cttgagcggc
     3001 ttgagcaggg gctgctgcgg cagagccgac ggtttctact tcgataatgg cagagccttc
     3061 ggacactttg tcgccgactt ttaagaatac ggctttaacg acaccggcag cggtacaagg
     3121 tacgtccatt gtcgctttat cggtttccaa agtaatcagc gtgtcgtctt cggcaacggt
     3181 gtcgcccact ttgatttcaa cggcgattac atccacatcg gtatggccgc cgatatcggg
     3241 aacggctact tgaacggttg caccgcctgc ggcaacgggt gcggcagcag ccgaggcggc
     3301 ttcggcagcg gcggcaccgg tttcaacggt caaaattacg ccgccttcgg agattttgtc
     3361 gccgactttg acttttactt ctttcacgac accggcagca tcggcaggca catccatcgt
     3421 ggctttgtcg gtttccagtg taatcagggt gtcgtcaacg gcgatggtgt cgcccgcttt
     3481 aacttctacg gcgatgatgt cgacgttttc gtgaccgccg atatcgggga ctttgatttc
     3541 tacgatactc atttgagttc ctttaatctt gttcagttgg atgccgtctg aaagctgctt
     3601 caggcggcat cggtacggtt tgtctgctca aaccattcgg gcattcggtt ttcagacggc
     3661 ctgatgtcgg aatggggcaa acagaccgtc tgaaacatca atcagcgttt ccagctagga
     3721 gctgaatcgg ctttgatgcc gtatttctca atggcttgtt gaacggtttc tttgctgact
     3781 ttgccttgtt ccgccaatgc gctcaatgct gccacggcaa cgttgtagcg atccacttca
     3841 aagaagcggc gcaggttggc gcggctgtcg gagcggccga agccgtcagt acccaagacg
     3901 tggtagtcgt tcgggatgta ggcgcggata cggtcagcat agctgcggat atagtcggta
     3961 gcggcaataa ccggaccgtc atgaccttgc aatttggaag taacgaaagg tactttttcg
     4021 gcttccagcg gatgcaggcg gttgaagcgt tctacctcga cagcgtcgcg gtgcagcagg
     4081 ttgaaggatg ggcaagacca aatgtctgct tccacgccga agtcggcttt cagcagctcg
     4141 gcaccggtaa ccacttcttg caaaatcgta ccggaaccca tcagttggac tttcttgtcg
     4201 cctttgccgc cggctttcag caggtacata cctttcaaga tgtcttgttc cgcaccttcg
     4261 ggcatatccg gatgggtgta gttctcgttc atcagggtga tgtagtagaa cacgtcttca
     4321 ttattggcat acatacggcg cagaccgtct tgtacgatga cggcgacttc gtattggaaa
     4381 gtcgggtcat aagatacgca gttcggaatc aggtcggcct ggatgtggct gtggccgtct
     4441 tcgtgttgca ggccttcgcc gttcagcgtc gtacggccgg cagtaccgcc cagcaggaag
     4501 ccgcgcgcgt gcatatcgcc cgccgcccaa gccaagtcgc cgatacgttg gaaaccgaac
     4561 atagaatagt aaatgtagaa cggaatcatc gcgaagtcgc tgttggcgta gctggttgca
     4621 gccgcaatcc agtcggccat cgcgcccggt tcgttaatac cttcttgcaa gatttgaccg
     4681 tcaacggatt ctttatagaa catcagttgg tctttgtctt gaggggtata ttgctgacct
     4741 ttcggattcc aaataccgta ttggcggaac ataccttcca tgccgaaagt acggctttcg
     4801 tcgggaacga taggtacgac gcgtttgccg atttttttgt ctttcagcaa agtggacagg
     4861 atgcggacga atgccatcgt ggtcgagaac tcgcgttcac cgctggattt gagttgtgcg
     4921 tcgaatgctg acagctcggg cacttccaat acttcctgcg tcggtttgcg ttgcggcagg
     4981 tagccgccca aagcatcgcg gcgtgcgtgc aggtatttgt attcttccgt atcgggggca
     5041 aaagtcaggt aaggcagatc gccgctttcg atttgttcgt cggtaaccgg aatgtcaaag
     5101 cggtcgcgga attgtttcag ggacgctttg tccatttttt tggcttggtg ggcaacgttc
     5161 tgaccttcgc cggatgcgcc cataccgtaa cctttaatgg ttttcgccaa gatgacggta
     5221 ggtttgccgt cggcatggtt cgctgcgcgg tcgtaggcgt tgtacacttt ttgcgggtcg
     5281 tggccgccgc ggttcaatgc ccagagttgc tcatcggtca tatcggcaac caatgctttc
     5341 agttcgggcg tattgaagaa gtgttcgcgc acatacgcgc cgtctttgga tttgtaagtt
     5401 tggtagtcgc cgtccaaaca ttcttccata cgttggcgca ggataccgtc tttgtctttc
     5461 gccaagaggc ggtcccagcg gcggccccaa atgactttga cgacattcca gccggcgccg
     5521 gcaaagttgc cttccaattc ttggatgatt ttgccgttgc cgcgtaccgg accgtccaag
     5581 cgttgcagat tgcagttgat gacgaaaatc aggttgtcca agccttcgcg tgcagccagt
     5641 gcgattgcac cttgagattc gggttcgtcc atttcgccgt cgccgcagaa acaccatact
     5701 ttacggcctt tggtttttgc caaaccacgc gattccaagt atttcaggaa acgcgcctga
     5761 taaatcgcca tgatgggccc caagcccatg gatacggtcg ggaactgcca aaagtcgggc
     5821 aagaggtggg ggtgcggata ggaaggcaga ccgtgtccgt ccacttcttg gcggaagttg
     5881 ttcagctggt cttcggtcag acggccctcg acgaatgcgc gtgcatagat gcccggggcg
     5941 acgtgacctt ggaagaagac caaatcgcct tcttcgcctt cgcctttggc tttccaaaag
     6001 tggttgaaac cgacttcgta catggtggcg gcagattgga aagatgcgat gtgcccaccc
     6061 agttccaaat ctttcttgcc ggcgcgcaat acgatggcgg cggcgttcca gcgcacgaat
     6121 gcgcggatgc ggtgttcgat gttttggtcg cccggaatgc ctttttcgtt ttcaaccgaa
     6181 acggtattca aatacggtgt ggtcgtgccg tgtggcatac gtacgccctt gtcgcggcag
     6241 tatttgacca ggttttccaa gaggtattgc gcgcgttcgc cgccttcata ttcgaggacg
     6301 gagcttaacg cgtccagcca ctcttgggtt tcgatagggt caacatcgtg taattgggtg
     6361 gacatagtat ctatccttta tgttgagtgt ttaatcggca gggagggtgt agccaacccc
     6421 tttattttcg tttgtgaaaa ataataagca aaaacgaaaa tttctaaatc aaaccgaaaa
     6481 aaataattta agtttaatgt tttcaaaaaa ttataaaagt tttggaaaca agggaaaact
     6541 tgaaaaaaat gttcgatctg ctccacacga aatcctatca gcaatcgggt ctgctggcaa
     6601 ataaccatgc ctgtaaaagc aggttttata ctgaagttgg cgtttggtaa ggtcaagtct
     6661 ttttgatttc gaaatattct gtttgatttt ggcgatattt tttcggaatt gtggtatatc
     6721 gaatcattat gtctctgtcg gcaattttcc tattgtagaa aatctataca caagctttgt
     6781 ggataaccgt gttgataagc cacggcttgt gttaaaaaag tcttacgcaa caaatgtacg
     6841 cttattttga ttaaaaaata ggcacattta aatctataaa aatcatgtgc ttatgttcaa
     6901 tatgatgttt ctggtcatgt ttttgccgtc tgttatggga tttctcccgc gcttggggca
     6961 gggggtgaat tttggaggtt ggtgcaaaac ctgctttttt cgcgttacaa tctgcacccg
     7021 atttactttt gaatttgcct gtttggaaaa atacttttat gaaagccagc caattcttta
     7081 tctctacttt aaaagaagcc cctgccgaag ccgcgcttgc cagccacaag ctgatgattc
     7141 gcgccggtct gattaaagcc aacgcgtccg gcctttatac ttggatgccg atggggctgc
     7201 gcgtgttacg caaagtcgaa aacgtcgtgc gcgaggaaat ggcgcgcgcg ggcagcgtgg
     7261 agctgctgat gccggtggtg cagcctgccg agctgtggca ggaatccggc cgctgggaat
     7321 tttacggtaa agaactgctg cgcctgaaag accgccacga ccgcgatttc tgcatgggcc
     7381 cgacctgcga ggaagtcatc gccgacatcg tgcgcaaaga aatcaacagc tacaaacagc
     7441 tgccgaaaaa cttttaccac atccaaacca aattccgcga cgaagtgcgc ccgcgtttcg
     7501 gcgtgatgcg cgcgcgcgag ttcgtcatga aagacgctta ttccttccat gccgactacg
     7561 cctcgcttca gacgacctat gatgccatgt atgactctta ctgccgcatc ttcacccgtc
     7621 tgggcttggc attccgcccc gtcgccgcag acaccggcag catcggcggt acgggttcgc
     7681 acgagtttca agtgttggcg gaaagcggcg aagatgttat tgcgtacagc gacacttccg
     7741 attacgccgc caatatcgag ttggcaccga ccttgccgct taaaggcgaa cgcgccgctg
     7801 ctcaggctgt gttgaccaaa gtacatacac caaacgtcaa aaccattgag tctttggttg
     7861 aattcctgaa tattccggtt gaacaaaccc tcaaatccat cgtggtcgaa ggcgaaaacg
     7921 aaggcgaaat cgtcttatta ctgttgcgcg gcgaccatga gttcaacgac atcaaggcag
     7981 aaaaactcgc aggcgtgaga tcgccattga cgatggcaag tcctgccgcg attgtcgaac
     8041 agttcggcgc aaacggcggc tcgctgggtc cggtcggctt tgcaggtaaa gtttatgccg
     8101 atttcgccac tgaaaaaggc gcggactggg tcatcggtgc gaacgaagac ggctaccact
     8161 ataccggctt caacttcggg cgcgatgccg ccgagcctga attcgtcgat ttgcgtaatg
     8221 tggtcgaagg cgacgaaagc cccgacgggc aaggccgtct gaaactggcg cgcggcattg
     8281 aagtcggaca cgtcttccag ttgcgcgaca aatacaccca agccatgaac gtaagcttcc
     8341 ttgacaacaa cggcaaatcg caaattatgg aaatgggctg ctacggcatc ggcatcaccc
     8401 gcgtcgttgc cgccgccatc gagcagaata acgacgaaaa aggcatcatc tggaccaaag
     8461 caatggcgcc gtttgaagtc gttatcgtgc cgatgaacta caaaaaatca gacaccgtac
     8521 gcgaagccgc cgacaaaatc tatgccgaat tgctggcggc aggcgcggat gtgctgctgg
     8581 atgaccgcga cgaacgcgca ggcgtattgc tgaacgattc cgagctgttg ggtattccgc
     8641 accgcatcgt catcggcgac cgcgctttga aagaaggcaa tgtcgaatac gccgaacgcc
     8701 gcaacaacga agcgcaggca gttgcaatcg gagaaattgt tgtgcgtgta acagctttat
     8761 taaatgggta agcttggtca aatgccgtct gaaaataatt tcagacggca tttttatttg
     8821 gggagaaagt gaagtggctt cagtattttt agaaggagaa gcagtcgagg tcggcaatat
     8881 tttctgtatc ggcaggaact atgccgcaca tattgaagag ttaaaaaacg aaataccttc
     8941 cgaacctgtg gtgtttatga agccgtcagg cagcattttg aacagcggcg gtacaatctt
     9001 gctgcccgag tttagtaggg atgtgcagtt tgagtgcgaa ctcgtgctgc tggtcggtaa
     9061 ggattcagac ggcacgggcg agggtaaaga tattttggga tgtgtcgcag gatatggtgt
     9121 agggctggac cttacggcac gggatatcca atgccgtctg aaggaaaaag ggctgccctg
     9181 gctgaaggca aaagggttca ggcattccgc gtgcgtgtcg gattttgcgg cggcaggcag
     9241 aatcggcaat cccgagaatg tgttgttttc attaaaacaa aacggcgtat tgaaacagcg
     9301 cggggatacc ggtttgatga tttacccgat tcgggaaatt ttacacaaac tggcggcaga
     9361 ttacggtttg gggaaagggg atttggtgtt taccggaacg ccctccggcg tgggcgcaat
     9421 cggtgcgggg gacaatttgg cattggagct ggacggcttg gtgcgcgcct ctttcacggt
     9481 agggtgttaa ccgcgccgct taaccctcac gcccgttgaa acattccgcc ggaccgccct
     9541 cgaaaaaacc gtgcaagatg gcttgcgccg ccacttggtc gagcaccgat ttgcgttttt
     9601 tgccgaagac ctgtgcttcc gaaagcaggc tttcggcata gacggacgac agccgttcgt
     9661 caacccaata gacggggaga ttgaacctgc cgttcagcct gcgtccgaac ttgcgcgaca
     9721 ggtgcgtcat ttcatgtttc gtgccgtcgg tatgcacggg cagtccgacg acaaaataac
     9781 gcggctgcca ttcttgaacc agcttggcga ttgccgcgaa cttttcatcg ttgctgccgc
     9841 cggtaacggt gctcaaagga tgggatagcc ctaattccgc gtctccttgt gccacgccga
     9901 tacgcgcttc gccgaagtcg aatgccagtg ccgttccttt tggaatttta tgcatggccg
     9961 gctcccgaaa acagggcgag cgggtcgatg ccgagtttgg cgaatgcggc ggcgtaacgg
    10021 tgttcgtagg ggatgtcgaa caggatgtgt tcgtcggcgg gaacagtcag ccacgcattg
    10081 tcggcaagtt cgcgttcgag ctggcctttt ccccagcttg aatagcctat gctgatcaag
    10141 gctttgtcaa ccgcaccttc gcgtgaaata ttttcaatca cgtctcggga agaagttagc
    10201 gcgatattgt ctgaaacgcc gatactgctt tgccagttgc cgatcggggt atgcacgaca
    10261 taacctcgct cgacttgcac cggcccgccc atcatcacgc tgtcgtgctg catccgcatg
    10321 gggatgtttt tgccggtggc ggaaaaaatc atgtccatcg taatcggaga gggtttgttg
    10381 atggcgatgc cgagtgcgcc gtcttcatcg tgtttgcaga tatagacgac cgattgtgaa
    10441 aaaaacgcgt cttccatatc gggcatggcg acgagaaagt ggttggagag gttcatatca
    10501 gcgtttgtcg ggattttcta tcgggttgga aatcatgcag gcgccggcaa gcaggttgcc
    10561 ggaagtatcg gcttgcaccc catcggtttg cggtatgccg ccgccgaaac aggaaaccgc
    10621 gcttaaggaa tttttcggcg agccttggat gattttatcg ctgtacacca aataggcgaa
    10681 ggttttgcgt ttggggtcgt aataacggac aatctgccgg ctcttgaacg cgaagctcgc
    10741 accgtgtttg aaaacttctt tcggtttgcg cacggcggtt tcgtcaaaag aaatcgaaga
    10801 tgccgtctga acgcacgaaa ccgatgcgtc ggacgcgtcc tcttccaaat tgaccatttc
    10861 cttcaagccg cctttttttg catacgaaat ataacaggca accccttgaa cgtcgggatc
    10921 gtcgaatcct tccacttcga tacggtcgtt tttgcccagt atgttgaaaa cggtacttgc
    10981 ccgtccgatt ttatcggttt cgccgctgcc gcaggcagtc agcaggacgg cggcagacag
    11041 cagtagcagt ctgttcatca aaatttcctt atcggttgaa tccccgtttt ccgtgaaggc
    11101 ggcagggctt gctttgtttg gaggggcgca cgttcccaaa atcgggcaac atacggtatg
    11161 cggcttaatg tgctaccctg tgtgtcggaa cacaatatcc gttgcggcat tgaaaatatg
    11221 acgcacaata taaggctgtt tgagccgatt gcaagtatcc gcgcaaagaa ggcgcaagat
    11281 tgggcaaacg gcattttcag acggcataac tgacagtata atccaaacgt ccggctgctt
    11341 tcgtgcagcc ttcagtatta tccaactttt tccgaaggtc gaaccatgcg ctacaaaccc
    11401 cttctgcttg ccctgatgct cgttttttcc acgcccgccg ttgccgccca cgacgcggca
    11461 cacaaccgtt ccgccgaagt gaaaaaacag acgaagaaca aaaaagaaca gcccgaagcg
    11521 gcggaaggca aaaaagaaaa aggcaaaaat ggcgcagtga aagataaaaa aacaggcggc
    11581 aaagaggcgg caaaagaggg caaagagtcc aaaaaaaccg ccaaaaaccg caaagaagca
    11641 gagaaggagg cgacatccag gcagtctgcg cgcaaaggac gcgaagggga taagaaatcg
    11701 aaggcggaac acaaaaaggc acatggcaag tccgtgtccg gatcc
//
LOCUS       DAWHXZ010000041        11639 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000041 DAWHXZ010000000
VERSION     DAWHXZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11639
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.041"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            89..370
                     /locus_tag="WIE35_000716"
     CDS             89..370
                     /locus_tag="WIE35_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ4153666.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDAATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(391..1617)
                     /locus_tag="WIE35_000717"
     CDS             complement(391..1617)
                     /locus_tag="WIE35_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ4153667.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(1632..2057)
                     /locus_tag="WIE35_000718"
     CDS             complement(1632..2057)
                     /locus_tag="WIE35_000718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153668.1"
                     /translation="MPSEHAAYADLTASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSECGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            2162..2821
                     /gene="queC"
                     /locus_tag="WIE35_000719"
     CDS             2162..2821
                     /gene="queC"
                     /locus_tag="WIE35_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ4153669.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQIYGRENVQAITFQYGQRHA
                     VELERASWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2849..3367
                     /locus_tag="WIE35_000720"
     CDS             2849..3367
                     /locus_tag="WIE35_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ4153670.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            3374..3796
                     /gene="queD"
                     /locus_tag="WIE35_000721"
     CDS             3374..3796
                     /gene="queD"
                     /locus_tag="WIE35_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ4153671.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRECQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            3905..4261
                     /locus_tag="WIE35_000722"
     CDS             3905..4261
                     /locus_tag="WIE35_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153672.1"
                     /translation="MNTTSSMIQEEGIIAESIDVINKFVQFLLKLYDDFGIDGMHDLV
                     DPDLDTLESIVKNLQQEVDKLPISPNDFSLENKKISLAQGLLYAQSMITNVRNKDTEE
                     CSRNRSMLKNNQSSLY"
     gene            4511..4882
                     /locus_tag="WIE35_000723"
     CDS             4511..4882
                     /locus_tag="WIE35_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ4153673.1"
                     /translation="MKLLFILLVLFVAVEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGVTVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            4879..5517
                     /locus_tag="WIE35_000724"
     CDS             4879..5517
                     /locus_tag="WIE35_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ4153674.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDASVHWQLSVQTHKWAGIE"
     gene            complement(5852..5928)
                     /locus_tag="WIE35_000725"
     tRNA            complement(5852..5928)
                     /locus_tag="WIE35_000725"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5892..5894),aa:Pro,seq:cgg)
     gene            complement(6009..7094)
                     /gene="nagZ"
                     /locus_tag="WIE35_000726"
     CDS             complement(6009..7094)
                     /gene="nagZ"
                     /locus_tag="WIE35_000726"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ4153675.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTGEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHSNPEAVTRLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLIPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELRDDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTAD
                     FQAAQAFVAALASPQDTAGGVKVGEAF"
     gene            complement(7151..8671)
                     /locus_tag="WIE35_000727"
     CDS             complement(7151..8671)
                     /locus_tag="WIE35_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ4153676.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETTVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            complement(8873..10372)
                     /locus_tag="WIE35_000728"
     CDS             complement(8873..10372)
                     /locus_tag="WIE35_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ4153677.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIKHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAENDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLRAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            complement(10510..11139)
                     /gene="nth"
                     /locus_tag="WIE35_000729"
     CDS             complement(10510..11139)
                     /gene="nth"
                     /locus_tag="WIE35_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ4153678.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(11185..11598)
                     /locus_tag="WIE35_000730"
     CDS             complement(11185..11598)
                     /locus_tag="WIE35_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ4153679.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
BASE COUNT         2799 a         3044 c         3202 g         2594 t
ORIGIN      
        1 tgggatttag ggctaatcta gtacagcccc ttgttttttc gatacggaac cggatagagg
       61 aaaaatcgaa cattgcgcct gccttgctat gattcacgaa gaaatctccg ccatgcctat
      121 gggctatgaa accttgatcg gcgatatggg cagcgcactg tcaggcggac aaaaacaacg
      181 catcgtattg gcgcgggcct tatattgcga accgaaaatc ctatttttag atgcagcgac
      241 cagccatttg gatattgcca atgaaaaagc agtcaatgca aacttgaatg gcttgtctat
      301 cataaaaatt atggcggcac acagaaagga aacggtggaa tcagcagata ggaaaatgtc
      361 tttaggataa aaatacagtt tcaaaaatac tcaagattgc tgctgttttt tcgcctgagc
      421 gtcaaactct gccagcgtca tgttcaaagt ctgcaaacac ggcatcatta ctgcatcgac
      481 agcttggttc acatgatccc tttccacagg caacggacgg taaacgaaga gcttgaagag
      541 ttcgttcaac tcaatcgaat ccgcccccgt tttcaacacc caaccctgtc tgccggaata
      601 gatatagccg taccgcgcca gcttttccaa aagctcgccc aactcgtcgt agcccatatt
      661 gatatgccgt ctgaactcct gaacaggcaa ggctttgcct tctttttgcg ccgcatccag
      721 aagcagcagg attttcaaca cgtcgtcaaa ccgtccgcgc gaatcgaagc ccctgcggaa
      781 cgcttctccc tgccagtagg agagtgaaga agtcagcacc gcgccgccca agaccagcgt
      841 ccacaacagg ttcagccaca acagaaaaaa cggcacggcg gcaaacgcgc cgtaaatcga
      901 gcggtagccg tcgaaattgc ccatatacca agtgaagagg gagcgcgcgg tttccagaca
      961 aaacgctgtt gccaaagccc cgacaaacgc ctgccgcgcg ggaacgaagc ggtttggcac
     1021 gaagcggtac agcccccaca gcaaaagcgt catgaaggtc agcgtcgccg ccgttcgcaa
     1081 cgcgcccgac cactgcggcg cacctgaggc aagcgcggca tcctgtaccg agccgaccat
     1141 aaaggaaatg cccacgccca aagacagcgg cccgaacgtc agtaaagccc aatagacgag
     1201 aaactgcatc atccacggac gctgggaatt gacccgccag atgcggttga acgtattgtc
     1261 tatcgtccga atcagcatca gcgaggtaac gaccagcatc acgctgccga ttgccgtcag
     1321 ccggttcgcc tgctcgcgga acgcattgat atagtcgaac accatgtccg cgccctgcgg
     1381 cacaatggtt tggttgacga aggagacgaa cgaatccgac cagcggtcga acacggggaa
     1441 aatcgaagcg accgccacca tcacggtcag cacggggacg agtgccagca gcgtcgtaaa
     1501 cgtcatgctt gccgccgcct gcggtacgcg ttcttcatca aagcggcgga cgacgaacca
     1561 tgcaaacgca cagattttat tgtctgccaa accttgcaaa cgttgtaaaa aggtcataat
     1621 ttcttgcccg gtcagtaagt tgggcattga tgcccgatgt tatagccaat tttgccgtca
     1681 ggaacaaatg cctgaactgc ggctgtttca gacggcatcg gaacaactgt tatgccgtct
     1741 gaagaccgaa ccattttaac ggaatccgcc catgaaccca aatccccctc aaaatcctcg
     1801 tcctctacta tttccaaaac ggcagcaccc gcaatcccgc actccgaatc gctcgcggca
     1861 tcgacagcgt tgaaggttgc gaagccgtat tgcgcaccgt ccccaaagtc tccgccgtct
     1921 gcgaagccgt caaaaaagat attcccgaca gcggctcccg tcctgaccgc cgaagaaaac
     1981 aatatcgcct tcgcacaaag caaacgcttg gcggaactcg ccgtcaagtc ggcataagcc
     2041 gcgtgttcag acggcatggc gttcagatgc cgtctgaacg cgtttgcctg tataatccgc
     2101 gtctttactg tccaacttcg cggttcgcaa acctcccgcg ttaccaaaac taggattcga
     2161 tatgtcaaac caaaaagcct tggtcatctt ttcgggcggt caggattcga ccacctgcct
     2221 gattcaggca atccaaatct acgggcgcga aaacgtccaa gccattactt tccaatacgg
     2281 gcaacgccat gccgtcgagc tggaacgtgc cagctggatt gcgcaggatt tgggcgtcaa
     2341 acaaaccgta ctcgacttga gcctgatgcg gcagattacg cacaatgccc tgatggacga
     2401 caccgccgcc atcgaaactg ccgaaaacgg cgttccgaat acctttgtag acggccgcaa
     2461 cgcgcttttc ctgctctatg ccgcgattta cgccaaaggg caggggatac ggcacatcat
     2521 cgcgggcgtg tgcgaaaccg acttctccgg ctatcctgac tgccgcgacg tgttcgtcaa
     2581 atccatgaac gttaccctta atctggcgat ggactatgat ttccaaatcc acacgccgct
     2641 gatgtatctg accaaggcgc aaacttgggc gttggcggac gaaatgggcg cgctggacta
     2701 tatccgcgag caaacccaca cctgctataa cggcatcgtc ggcggctgcc gcgaatgccc
     2761 gagctgtatc ttgcgcgaac gcgggctggc ggaatatctg gaaagtaaaa aggccgtctg
     2821 aacacgcgca aaccataagg aatacgatat gcccaagctc catatgtttt acctcggcgg
     2881 caatgccggc aggtcgaata tcgaagtgca cgacatccaa tttgccgtgt gcgacgacta
     2941 ccgtgaagcc gtccccgcac tcaaagccgc gtggttcggc gatgcggaca aaatccacat
     3001 cgacggctgg caggttgtcg aatgggcgga cggttacgac atcgccgtat ccgaaacgcc
     3061 caaaacgaaa atgccgtctg aacacgcccc gcgcctgtat ttcgccaatg tcggcggtta
     3121 tcgcgcaggg cagctcgccg aagcgcacgc ttttgggctg ttcgccgccg ccacgcctgc
     3181 cgaagccaaa caaaaagccc tgcaaaccct gttgaccgac agctatgttc agcaacataa
     3241 agacaactta aaagacgtgg acaacctgct tgcgctcgac cgcatcggca atttccatat
     3301 ccgcctgacc ccgaatccgc acggcaaacc cgccgaaatc ggctttcaag gctatttgcc
     3361 catttgagaa cccatgaaaa tcaccaaaat cttcaccttc gactcctcgc atatgctcga
     3421 cgggcatgac ggcaaatgcc aaaacctgca cggacatacc tacaaactcg aaatcaccgt
     3481 ttcagacggc attatcaaag gcggcgcgaa agacggtatg gtgatggact ttaccgactt
     3541 gaaagccatt gtcaaacaac acattaccga ccccttcgac cacgccttca tctaccacgg
     3601 cggcaacagc cgcgaatgcc aaatcgccgc gcttttggag ggctggaaca tgaaaaccct
     3661 gcgcctgccc tgccgcacca ctgccgaaaa tatggcggtc gaaatgtacg accgtctgaa
     3721 aaacgcgggg ctgaacgtgt gccgcgtcaa attgtgggaa acgccgacat cgtgtgcgga
     3781 gtatgaaggg gagtaatatt gaaacaaatt ttaaagattt gttttgactt gtcatataat
     3841 caattgtaaa attccatact ttctaaaaaa tcgaccgtaa tagaaaattt caggcataat
     3901 aaccatgaat acaactagta gtatgattca agaggaaggc attattgccg agagcataga
     3961 tgtaattaat aagttcgtac aatttctttt gaaactgtac gatgattttg ggattgatgg
     4021 tatgcatgat ttagttgatc ctgatttgga tactttggaa tccattgtga aaaatttaca
     4081 acaagaggtt gataaacttc cgatcagtcc caatgatttt tctttggaaa acaaaaaaat
     4141 tagtttggct caaggacttc tctacgcaca atctatgatt actaacgtta gaaataaaga
     4201 tactgaggaa tgttctcgga atcgttctat gctcaaaaat aatcagtcga gcctttatta
     4261 acaacagcaa ggagttattt atgaaatcaa tggatatccg tttcgctcgc attgctgcat
     4321 taacgaagca acttgcagct attcaaagac aaattgaaaa acgagcttat aaagcaaaag
     4381 ctgcaaccat gaaaaaagta gcatgaaaag ttttggtaga gaagccatcc ataatgggtg
     4441 gcttttttgt taaataaaat tggtaaagtt tgttaaatat aaatcttagt gcaaattaag
     4501 gtgcttaccc atgaaactcc tcttcatcct cctagtcctc ttcgtcgccg tcgaacattt
     4561 ctacatcgcc tggcttgaaa tgacacagat tcccagcgaa aaagcggcgg aaacgttcaa
     4621 gctgccttat gaatttatgg aacaaaatcg cgtgcagacc ctgttcggca accaagggct
     4681 gtataacggc tttctcggca tcgggctggt gtggtcgcgg tttgctgcgc cggataacgc
     4741 ggtgtacggc gtgacggtac tgtttctcgg cttcgtcctg attgccgccg cgtggggcgc
     4801 gttctcttcc ggcaacaaag gcatactcgt caaacaaggt ttgcccgcat ttttggcagc
     4861 ggcggcggtg ttggcggtat gaaaaaaatc aatgttgccc ccgaaaatcc ccaataccgc
     4921 atcgtcgaaa tctttgaaag cctgcaaggc gaaggctgga acacgggcat gcccgccgtt
     4981 ttcgtccgct tgggcaaatg caacctcgcc tgcggctggt gtgataccga ttatttgaca
     5041 ttcggcatga tgagcctgtc cgacatctta ggctgtctga aaacctatgc cgcccgcaac
     5101 atcatcatca ccggcggcga accgaccata cagccgcatc tcgatatgct gctggacacg
     5161 ctcaaggcgg aaggctattt cctctgtctc gaaaccaacg gactcaatcc cgcgccgccg
     5221 caaatcgact acgtcgccac cagccccaaa gcctgctacg ccgccaaata tgaaaatagc
     5281 tgtatcgaaa cagccgacga agtgcggatt gttgccgatg gtgatgtcct tgcgttctgc
     5341 gaaaacatgg aacgcaaaat ccgcgcacat cattactacc tttcgccctg tgagcaagac
     5401 ggtgcgatga acatctacga caccatccgc caaatcggta ttttaaacag tcgccccgac
     5461 gcatccgtgc attggcagtt gagcgtgcag acgcacaaat gggcgggaat agagtagttt
     5521 aagcagtgta actcaaaggg ccggcgtacc gttttaccga tgtttgacat acggggaaag
     5581 tgtgccgctt ctgcgtggaa atgccggtat ttccaccgcc caatcaggac ggagccttac
     5641 tgaataagat gctgccgttg ggtacaagcc cggcttccta aattccgatg gtcttttgaa
     5701 ccttgccgat accctttgtc agtgcacgca aatagaaggg ttagggaaaa cgaaatgccg
     5761 tctgaaacag aatgctgttt cagacggcat ttttctgttg acgccaaaag gaaaaaccgc
     5821 tccggcaatg gatgccgagg cggtttggat ttggtcggaa tgagaggatt cgaacctccg
     5881 accccttcgt cccgaacgaa gtgcgctacc gggctgcgct acattccgaa ttaagtaagg
     5941 cgtgattata gcgcaaaaaa tacggcgtgc ctataccgtt ttgccttttt gccgcgtgtc
     6001 gggcggattt aaaaggcttc gccgactttt acgccgcccg ctgtatcctg cggcgaggca
     6061 agggcggcaa caaaggcttg cgccgcttgg aaatccgccg tctgcataac ggcttgcgcg
     6121 gcggcgctgc cgagcgtgtt ggccatgtat tgccaacgtt gcgccaaagc gggattgtcg
     6181 ggaatgcgga aatcatcgcg cagttcgtcc acaaggtcgg gacggttgca gacgaggaca
     6241 atgtcgcaac ctgcctcaaa ggaaatgcgg gcgcgttctt tgatgccgcc tgccccgcac
     6301 gcgccctcca tagtcaaatc gtccgagaaa atcacgcctt tgaacccgat gtcgcggcgc
     6361 aaaatctgtt tcagccagat ttcggaaaac ccggcgggct ttgtgtccac ttgtggataa
     6421 acgacgtggg cgggcataac cgccgccata ccttcgcggc tcataatgcg gaaaggaatg
     6481 aggtcggcgg cttcgagttc gtcgaggctg cgcccgtctt cgggtagcac caaatggctg
     6541 tccccttcga caaacccgtg tccgggaaaa tgtttgccgc aggatttcat accgcctttt
     6601 gccaaacctt tttgaagggc gagggcgagg cgggttacgg cttcggggtt actgtggaaa
     6661 ctgcggttgc cgatgacggg gcagtttccc cagtccaaat ctaagacggg cgtgaaggac
     6721 aagtcgatgc cgcaggcgga aagctcggtt gccaaaaccc ggccgacttg tccggcggcg
     6781 gtttcggcgg cggacgcgcc gtctttgtcc caaatctcgc cgagcgtact cattgcgggc
     6841 aggcgggtga agccttcgat gaaacgttgc acccttccgc cctcgtgatc gacggcgatg
     6901 atgagttcgg gtgtgcggag ggctttgatt tcggcggtga gtgttttgag ctgttcgatg
     6961 ttttggaagt tgcggcggaa gaggatgatg ccgcctacgg cgggatcgag caggcgttgt
     7021 ttttcttctc cggtcaggcg gaaggcggca atgtctgcca tgacggggcc gcgcggaata
     7081 tgggggacgg tcattgcggt ttgctccaaa aagcttcaga cggcatatgc cgtctgaaca
     7141 gggaaagggg tcaggcgttg gcgcgttttt tatctttcaa cagaaaaatc agcaccgcca
     7201 atacaatgcc tgtcgtgcca aaacccaaca gcgcggattt tgtcagaccc aatgcgaggt
     7261 agcccgatgc ggcggcggcg gcaacggtta aggcgtaagg cagttgcgag gtaacgtggt
     7321 cgatgtggtt gcagcgcgct ccggtggacg acaggatggt cgtgtcggaa atgggcgagc
     7381 agtggtcgcc gcataccgcc cccgccatta ctgcggacat acacgggata atcagcgcgg
     7441 gttcgacttt gaccgccatg gcggcggcaa tcggcagcat aatgccgaac gtcccccagc
     7501 ttgtgcctgt ggcaaacgcc atcacgctgg cgagcaggaa gaggatgacg ggcaggaagc
     7561 cgggatggat gttgcccgca accagcgtgg agaggtaatc gccggtgtgc atttcgccga
     7621 caaccgtact gatgagccaa gcgaggatta aaatggcgat tgcgccgaac atagatttcg
     7681 caccctgcca aacggctttg ggatagtcgg cggttttaat cgtgccgagc gtgcagagaa
     7741 cgacggcaag gacgccgcaa gtgccgccga ataccagcga agtgtttacg tcggtatttt
     7801 caaatgcccc caaaatgctg aaggtttcgc ttgcctgcgc gccggtgtag atcatggcgg
     7861 aaaccgttga ggcgattaag gccaaaacgg gaataatcag tgcgtaaaca cgacctttgg
     7921 tagcgtctga aacggtagtt tcatcgtggg cttcgttcaa cgcggcttgt tcgaaacgtg
     7981 ccatcgagcc gatgtcgaag gaaaaccatg cgacgacgaa caccataatc agggcaaaca
     8041 gtgcgtaata gttcatcagg ctcatggcga caaacgtccc catcggcgtg tattcggtga
     8101 ttttgtaggt aacgagcagt ccggcaagcg tggcgataat cgacgcgccc cagcttgaaa
     8161 cgggcatcag cacgcacata ggcgcggcag tggagtcgag gatgtaggcg agtttggcgc
     8221 gggaaacttt aaacttgtcg gtaacggggc gggcaatcgc accgacggcg agactgtgga
     8281 aatagtcgtc gataaaggtt acaaacacga ggcaggcggt cagcattttc gcgccgcgcc
     8341 ggtttttaat gtgccgtttt gcccagtcgg caaacgcctg attgctgccg gagtaggtca
     8401 gcagggaagt aaaaataccc aaaagtatca ggaaaaccaa gatttttggt ttgcccagcg
     8461 accaatcgcc gtctgaccaa gccaagccga cgaccatgtc tttcaggtgt gtcagaccgt
     8521 cgacggggtt gccgccgacc aaaaaggcaa cgccgaccag aataccgatg cctaaagaca
     8581 gcagtacgcg gcgggtaatg acggcaagtg ccagtgccaa aaagggtggc acaaccgaga
     8641 aaaatgaatg tgaatagtcg atcagctgca tggttatggg ggtattaagc gtccggatgg
     8701 gagcgtatct gtccgcctcc ggtttgggtt ttgttggcaa aatgggcgga aatatttttt
     8761 gtcgtaaaaa atatttgttt aaaatcaacc aactgatttt tgtaaaatgc ccgttaatcg
     8821 gtattgacgg gcattttatc atttaaaaaa tattttggtt aaattatgtg ggttattgca
     8881 ggtttaatgc gataaacagc gtgttgccac ggcgcatgac cagcaggggg acgtttttgc
     8941 ctgccttgtc catagctttg cggaaaccgg cttcgtcatt gacggggact tgcccgacgg
     9001 caagaatttc gtcgccgcgc ctcaagcctg cgcgttctgc cgcgtcggaa acccgtacga
     9061 cgacgaggtg tccgccgctg ctgtcggtat gtgtctgaag ggtaatgcct gcggattcga
     9121 ccgagaacgt accggattgc tgttcggtgt agggggcttc atctgttttg gatgatgcgc
     9181 cgatatgctc ggcggcgttg cccagcttga ctttgattgt gatttcttcg cctttgcgcc
     9241 atacgccgag gctgacttct tttcccggcg taatggcgcc gaccataacg ggaaggtcgc
     9301 cggaagaacg tatttctccg ccgtcgaggc tgaggacgat gtcgcccgcc cgcaggccgg
     9361 cacgttctgc ggggctgccg ggcaggattt tggcaatcag tgcgccgccg gctttgtcca
     9421 aaccgaacga ttgtgccaaa ccgtaggata cttcttgaat aatcacgccc agttgtccgc
     9481 gttggacttt gccggtgttt ttcagctgtt cggcgacatt catggcaacg tcaatcggga
     9541 tggcgaagga aatgcccatg aatccgccgc tgcggctgta tatttgcgag ttgatgccga
     9601 cgacctgtcc ttttaagttg aacagcgggc cgccggagtt gcccggattg atggcaacgt
     9661 cggtttggat gaagggtgtg tagctttcgt tgggcaggct tctgcctttg gcggacacga
     9721 tgccggcggt cacgctgttg tcgaagccga agggcgcgcc gatggcggcg acccattcgc
     9781 ccggtttcaa atctttggga ttgccgattt tgacgacggg cagctcttcc gttgcgtcga
     9841 ttttcagaag ggcgacatcg gattggacat ccgaaccgat gagtttggcg gtatattcgc
     9901 gcttgtcgtt gagcaggact ttgatactgc ccatgccggt aacgacgtgc gtattggtca
     9961 gaatatagcc gtctttgctg atgatgaagc ccgaaccgaa gttcaatccg ccgtcatctg
    10021 cttcttcttg ggggatttcg ggcatgttcg ggacgaggcg tttgaaaaat tcgtagaacg
    10081 ggtcgctgtc ggcaagcggg tcggaatcgt tttcggcatt gctgctgccg ttttgggtgc
    10141 gcggggcggg ggctgcctga atattgacga ctgccgggcc ttcgctttga accagttgga
    10201 caaagtcggg cagcagcata ctgacgctgc cgtcgtcttt ggtgtgtttg atgcgttcta
    10261 cgaaggatgc ttcttttttg tccgcaccga aaaagctgcc ggctttgtcg cagcctgcca
    10321 gcgaggcggc acacagtgct gccaaagcga ggtattggta ttttttgaac acgttttgtc
    10381 ctttgtcgga tgccggtacc ggctttaatg ccgtctgaag cgcattttgt cggcttcaga
    10441 cggcataggt tgaaattcta caacgtccgt ccgaattttc aagcgtttca ttttgaaggg
    10501 cggcggcggt caggctttgg cgggatattc gcacaaatcg ttgatgatgc aggtttggca
    10561 ttgcggtttg agtgccttgc aggtgtagcg tccgtgcaaa atcagccagt ggtgcgcgtc
    10621 catcagaaat tctttaggaa tgaagcgcat cagtttgtct tcgacttcgc gcacatcttt
    10681 cccgggggcg attttggttc ggttggacac gcggaaaata tgcgtatcga ccgccataac
    10741 ggggtgtccg aacgctgtgt tcaataccac gtttgccgtt ttgcgcccca cgcccggcaa
    10801 cgattccaaa gcctcgcggt cttccggcac ttcgccattg tatttttcca gcaggatgcg
    10861 gcaggtttgc atgatgtgct tggacttggt tttatacagc ccgatggttt tcgtgtattc
    10921 catcacgccg tccaaaccca aatccagcat cgcctgcggc gtatcggcaa ccggaaacag
    10981 cttcgccgtc gctttgttta cgccgacgtc ggttgcctgt gccgacagta aaacggcgat
    11041 taaaagctcg aaaggggagt tgaagttgag ttctgtggtc ggatgggggt tggcggcgcg
    11101 gaagcgttcg aagatttctt ggcggatgtg tctgttcatt tttttatacg gtgggtttgt
    11161 gtgttcggca ttataacgta tggttcaggc ggcgtaatat tgcattcccc acagaatgaa
    11221 ggcgtaacgc gccgttttgc cgataaccag catcagcccg cttgtccacg gattcaaccg
    11281 cagccagccg gcggcaagcg gcagtgcgtc gccgacgacg ggcagccagg caaacgcaag
    11341 cagccaaata ccgaaacgcc gcatcagatt cagtgttttt tcagacggca tttttcggga
    11401 gggcagcaaa cgccccatcc aataggaaac catactgccc aatccgttgg caaggccggc
    11461 gcacagcaac gcgccgtatg cgtgttcggg aaagcggtgg acgaacaggg caaaggcggc
    11521 ttcggatgtg ccgggcagga gggtggcgga agtgaaggcg gaaaaagcga gcgcggcgta
    11581 ggcgtaggcg ggcatcattg caaacagtct caaacaggca aaaatcggcg acggattgt
//
LOCUS       DAWHXZ010000042        11627 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000042 DAWHXZ010000000
VERSION     DAWHXZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11627
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.042"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            14..285
                     /locus_tag="WIE35_000731"
                     /pseudo
     CDS             14..285
                     /locus_tag="WIE35_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            325..1359
                     /locus_tag="WIE35_000732"
     CDS             325..1359
                     /locus_tag="WIE35_000732"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ4153680.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            1565..2146
                     /locus_tag="WIE35_000733"
     CDS             1565..2146
                     /locus_tag="WIE35_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ4153681.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            2215..2397
                     /locus_tag="WIE35_000734"
     CDS             2215..2397
                     /locus_tag="WIE35_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ4153682.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARPLGEEELEA"
     gene            2394..3155
                     /gene="kdsB"
                     /locus_tag="WIE35_000735"
     CDS             2394..3155
                     /gene="kdsB"
                     /locus_tag="WIE35_000735"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ4153683.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRIRAVFQTI"
     gene            3178..3495
                     /locus_tag="WIE35_000736"
                     /pseudo
     CDS             3178..3495
                     /locus_tag="WIE35_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            3600..3797
                     /locus_tag="WIE35_000737"
     CDS             3600..3797
                     /locus_tag="WIE35_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153684.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDAAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            3954..4046
                     /locus_tag="WIE35_000738"
     tRNA            3954..4046
                     /locus_tag="WIE35_000738"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3988..3990,aa:Ser,seq:gct)
     gene            4349..5134
                     /locus_tag="WIE35_000739"
     CDS             4349..5134
                     /locus_tag="WIE35_000739"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ4153685.1"
                     /translation="MSRIRQAFAALNGEKALIPYITVGDPDIGATLAIMHSMVENGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAVVGALVKELKDAVR"
     gene            5171..6043
                     /locus_tag="WIE35_000740"
     CDS             5171..6043
                     /locus_tag="WIE35_000740"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ4153686.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPIDPLKFKDSKKYPDRLGAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(6103..6714)
                     /locus_tag="WIE35_000741"
     CDS             complement(6103..6714)
                     /locus_tag="WIE35_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ4153687.1"
                     /translation="MRRTALLLLALAAGTSLATSFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIIPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSMKPHNK"
     gene            6823..7047
                     /locus_tag="WIE35_000742"
     CDS             6823..7047
                     /locus_tag="WIE35_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ4153688.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGVPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            7410..8444
                     /gene="pyrC"
                     /locus_tag="WIE35_000743"
     CDS             7410..8444
                     /gene="pyrC"
                     /locus_tag="WIE35_000743"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ4153689.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATPALVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASIPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(8810..9235)
                     /gene="nusB"
                     /locus_tag="WIE35_000744"
     CDS             complement(8810..9235)
                     /gene="nusB"
                     /locus_tag="WIE35_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ4153690.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(9313..9789)
                     /locus_tag="WIE35_000745"
     CDS             complement(9313..9789)
                     /locus_tag="WIE35_000745"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ4153691.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(9837..10160)
                     /locus_tag="WIE35_000746"
     CDS             complement(9837..10160)
                     /locus_tag="WIE35_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ4153692.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNQPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            10327..11046
                     /gene="rnc"
                     /locus_tag="WIE35_000747"
     CDS             10327..11046
                     /gene="rnc"
                     /locus_tag="WIE35_000747"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ4153693.1"
                     /translation="MKDDVLKQQAHAAIQKRLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDTFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            11094..>11627
                     /gene="era"
                     /locus_tag="WIE35_000748"
     CDS             11094..>11627
                     /gene="era"
                     /locus_tag="WIE35_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ4153694.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLE"
BASE COUNT         2645 a         2824 c         3416 g         2742 t
ORIGIN      
        1 aagatactgg aaaatgaaag agatgaaacc tgtccgttat catattggcg atatgcccga
       61 gacttcaaaa caaaccgcct cccgtcatga cgacagggca gtgggtgttg acgatgattg
      121 ttttcatgat tcctttggtc aatttttgtt tgggtgttcg gcagaggcaa cccgaaccgc
      181 gccaatttct gtaaagcgca gttgcttatt tacctgattg gttcgcttat cggtttggtc
      241 ttcgcgttgt ttataggtgg gtctgtatcg ggtacgcatg attaatgccc cgggctgatt
      301 ttgcttcgag gatttgtatc gaatatgccg aattgtttca aatttcatac tgttatcgaa
      361 cggcattggc aaaaacctta tccggttttg tcttttctgc ttaagccgct ctccgggctg
      421 tttgccaaaa ttgcggcaaa acggcggacg gattttttat cgggaaaacg gcaaagcgaa
      481 aagctgcccg tgcctgtggt cgtggtcggc aatattcacg cgggtgggac ggggaaaacg
      541 ccgattgttg ccgcgctggt gtcgggtttg caggaaaagg gcgtcaaggt cggcatcatc
      601 agccggggct acgggcgcaa gagcaaggcg gttcatgtat tgaatgctga gagccgagcg
      661 gaagatgcgg gcgatgagcc tttgctgctg ttccgcaaaa ccggtgcgcc gacggcggtg
      721 ggcagcagcc gtgcagaggc aggcagggcg ttgctggcgg cgcatcccga catcggactg
      781 attgtggcgg acgacggttt gcagcattac gccctgcggc gagatgtgga aatcgcggtg
      841 tttccggcgg cggatacggg gcgcacggat ttggatttac tgcccaacgg cagtttgcgc
      901 gaacctttgt tgcggctgga ttcggtggat gcggtcgtcg tcagcggcgg caaggcggat
      961 gcgctgttta ggccgtctga aaatatgttt cacagccgta tcgaagcggg acggatttac
     1021 cgtttgaaca atccgtccga aatactggac acaggccgtc tgaaaaatca aaccgtcgtc
     1081 gccgtggcag gtattgccaa gccggcgcgt tttttcgatt cgttgcggaa tatgggcatt
     1141 accgtgaagc gaaccgtcgc gctgcccgac cacgccgaca tttcggcggc agatttgccc
     1201 gatgcggacg cggtcattat tacggagaaa gatgcggtca aattttcaga cggcatttgc
     1261 accgataatg tttgggtgtt gcccgtttgt gcgataatcg aacctgattt ggcggcgttt
     1321 gtgttggagc ggttggaaga tgtaccgaag gccgtctgaa agcacggttt gggcggagtg
     1381 attacggatt tgaataagaa cgcctcgcgc cgtcattccc gcgcaggcgg gaatctaagt
     1441 ctcgaatttt caggaatgcc taggaggctt cagaaatccc aaatctccgg atttccactt
     1501 ggacaggaat gagaaaatcg gtcgtatttt ttatctgcat taatcattcg ttaaagtatt
     1561 gaatattaaa ctgaaaacct tgttattgcc cttcgccacg ctggcattgt gcaccaacgc
     1621 ttttgccgcc ccgcctagcg acgcgtcgtt ggcgcgttgg ctggatacgc agaattttga
     1681 ccgggatata gaaaaaaata tgattgaggg ctttaatgcc ggatttaaac cgtatgcgga
     1741 caaagccctt gccgaaatgc cggaagcgaa aaaagatcag gcggcagaag cctttaaccg
     1801 ttatcgtgag aatgttttga aagatttgat tacgcccgaa gtgaaacagg ctgtccgtaa
     1861 tactttattg aagaatgccc gtgagatata cacgcaagaa gaaattgacg gcatgattgc
     1921 cttttacggt tcgcctgtcg gtcagtccgt cgttgccaaa aatccgcgct taatcaagaa
     1981 atcgatgagt gaaatagcag tatcttggac tgcattgtca gggaaaatcg cgcgacatca
     2041 tctgcccgcg tttacggaag agttgcggcg catcatctgc ggcggtaaaa atcccgatgc
     2101 gggctgtaaa caagccggac aggttgggaa aaggcatcag aaataaatga tagccgtctg
     2161 aaatattgaa gagggcatcc gattgattga accatcaaac ccgaaagcaa ccctatggaa
     2221 aaaaaattct tagacatcct cgtctgcccc gttaccaaag gcaggctgga atatcatcag
     2281 gacaaacagg aattgtggag ccgtcaggcg aagcttgcct atccgattaa agacggcatt
     2341 ccctatatgc tggaaaacga agcgcgtccg ttgggcgaag aggaactcga agcatgaccg
     2401 aattcgtcgt attgattccg gcgcggctgg attcgtcgcg cctgcccgga aaagccttgg
     2461 cggacatcca cggcaaaccg atggtcgtgc gcgttgccga acaggcggca aaaagtaaag
     2521 ctgtgcgcgt cgtcgttgcc accgaccatc ccgatattca gacggcctgt caggcgcacg
     2581 gcgtcgaagt cgtcatgact tcaaaccggc acgaaagcgg cacgacgcgc cttgccgaag
     2641 cctctgccgc gctgaagctg ccgccgcatc tggtggttgt caacgtacag ggcgacgagc
     2701 cgctgattgc acctgaactc atcgaccgca ccgccgaagt actcgtcgaa aacaacgtcc
     2761 aaatggcgac cgccgcccac gaattgcacg atttcgacga attgatgaat cccaatgccg
     2821 tcaaagttgt cctcgacaaa aaccgcaacg ccatctactt cagccgcgcc ccgattccct
     2881 atccgcgcga tgcgatgcgt gcaggaaaac gcgaaatgcc gtttgaaacc gccgtcctgc
     2941 gccatatcgg catctacgcc taccgcgtcg gcttcctgca acgctacacc gaaatgagcg
     3001 tttcgccgct ggaaaccatt gaatcgctgg agcaactgcg tgtcctgtgg cacggctacc
     3061 cgattgccgt cgaaaccgcc aaagaagctc ctgccgccgg cgtagatacg caggaggatt
     3121 tggacaggat tcgcgctgta tttcagacta tataaaatag attcaaaggg aaaagatatg
     3181 cagcaacata ttgaaaaatg gcaacacttg agccgggaag aacagaaaat ccttgctgaa
     3241 gtatggggtc tcgtgcaaaa cgacgatcag gaggttcact atgaaatgct caaattgaac
     3301 gcacccgacg aagccagtgg cgaattttgg ttcagaatgg cagaaacact cagcaccctg
     3361 ccgcccaacc gttccctcga ccttagaatg aacggcggca ggctggcgac cgccgtatcc
     3421 atcctttccg tcatgattga agacaatccc gacataccgc agctttgggc gcaaaaaatt
     3481 accgcgctca attatagtgg attaaattta aaccagtacg gcgttgcctc gccttgtcgt
     3541 actatctgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactatatt
     3601 tggcacacgg acacaaagcc cgtgccgacg gtttggcaca acagcccgac aaagcggcag
     3661 aagccaacga ggaggaatac ctgaccaaag ccctgtcgca aaacctactg tcaacattgg
     3721 atgccgcgct tgcacgtttt cctgaagacg cgtggtttca ggaaatcaaa caggatgcac
     3781 aaaagcattt tgcttgagga tgtggcagtc aggaatattt ccattcagga agaaaagaag
     3841 tgcctgattg ggtataatca gggtaaatct tattttattt caaaagatta atatttgctt
     3901 tctgtttttc cttgacggta tcggaaaagt tgattatagt tacagcttcc ttaggagtaa
     3961 tggctgagag gctgaaggca cttccctgct aaggaagcat gtggggtcaa cctgcatcga
     4021 gggttcgaat ccctcttact ccgccaagat gagaagacgc tgtgttttac agcgtctttt
     4081 tttacgcgct ttgttgcgga agtgccgagg gctgtctgcc gtgcgccgcc tggatttgca
     4141 atgtgcccgc ccgtgcaaaa tccgatttga ccaacctgaa gggcaggttt cggaagagcg
     4201 gggaattttg ataaacaaaa aactgcacgg cataatcttt cccggttttc attttccgta
     4261 ttcgcctatc cgtaccgcaa atgctatact gggaaaaatt taccgattgt gttttacggc
     4321 atatttgccg ataggatgga agagacaaat gagcagaatc cggcaggctt ttgccgcttt
     4381 aaacggtgaa aaggcattga ttccttatat tacggttgga gacccggaca ttggggcgac
     4441 tttggcaata atgcacagta tggtggaaaa cggtgcggat attttggagt tgggcgtgcc
     4501 gttttccgat ccgatggcgg atggaccggt tattcagcgt gcggcggagc gggcgttggc
     4561 aaacgggatt tcgctgcgcg atgtcttgga tgtcgtcaga aaattccgtg aaaccgacac
     4621 gcaaacgccg gttgttttga tgggatattt gaaccctgta cataagatgg gttatcggga
     4681 gtttgctcag gaagccgcaa tggcgggtgt ggacggcgtg ttgacggtgg attcccctgt
     4741 cgaaaccatc gatccgctct atcgcgagct gaaggataac ggggtcgact gtattttcct
     4801 gattgcgccg acgacgacgg aagaccgtat taaaaccatt gccgagctgg caggcggatt
     4861 tgtctattat gtttcgctca agggcgtaac gggcgcggca agtttggata cggatgaggt
     4921 ttcgcgtaaa atagagtatt tgcatcagta tatcgatatt cccatcggtg tcggtttcgg
     4981 catcagcaat gcggaaagcg cgcgcaaaat cggccgggtt gccgacgcgg tcattgtcgg
     5041 cagccggatt gtgaaagaga ttgaaaacaa tgcgggcaac gaggctgcgg tcgtcggtgc
     5101 tttggtcaaa gagttgaagg atgccgtgcg ctgattctgt cacgcattct aaatatttta
     5161 ggagttgtcc atgagctggt tagataaaat cctgccaccc aaaatcaaaa accgcagcaa
     5221 agacggttct tccaatgttc ccgagggttt gtggcacaaa tgtccgtctt gttcggcaac
     5281 cgtttattcg accgagttgc agcagaacaa tcaggtttgt ccgaaatgca accaccacaa
     5341 tccgttgtcg gcacgacaac gcctgaacct gcttttggat gaggatggca gggaggaagt
     5401 tgccggaaat gtcaaaccga tagatccttt gaagtttaaa gacagtaaaa aatatcccga
     5461 ccgtttgggg gcggcacgca agctgaccgg ggaagatgac gcgctggtgg tgatgaaggg
     5521 gatgatgaac ggcctgcccg tcgtcgttgc tgcgtttgaa ttccgcttta tcggcggttc
     5581 gatgggttcg gttgtgggag agcgtttcgt acagggcgtc cgccgtgcgg ttgccgacaa
     5641 ttgtccgttc gtctgtgtgg cggcttccgg cggtgcgcgt atgcaggagg gtgtgaactc
     5701 gttgatgcag atgacgaaaa ccagtgccgc gctgcatttg ctgacggaaa aacgcctgcc
     5761 gtttatatcg gtattgaccg atccgactat gggcggcgta tccgccagct tcgcattttt
     5821 gggtgatgtt gtgcttgccg aaccgaacgc gctgatcggt tttgccggtc cgcgcgtgat
     5881 tgagcagacg gtgcgcgaaa cgctgccgga aggcttccaa cgcgccgagt tcctgctgga
     5941 aaaaggcgca atcgaccaga ttgtcgaccg ccgcgatatg aagcggcgca tcagtgattt
     6001 gattacgctg ttgtgccgtc aggacaaagt ttccgccgct tgatggctga tgaatcgaat
     6061 accgtctgaa accgatgttt cagacggtat ttttgtgtct ggttatttgt tgtgcggctt
     6121 catcgacggg gcatagcgtc cggcacgttc tttcaggcgt tgtaccaaac ctttcgtgtc
     6181 ggcgggtaca ccgccctcgc agaatgcctg atacaggacg gtgcgcagtg cgtcgttgcg
     6241 gcttaatgta ccgcctatcg gtttccattc ggcgtttcgg ggctgtatcc agcggcggtt
     6301 gaccgtgtcg ccgtagccga acactttata ggaggaaggt ttgccgttgc cgaacctgat
     6361 ggagaagcgg gcgcagaata tgccttcggc agtcaggttg tcgtagcctt tgtcggagcg
     6421 gatattgaga atgtagcgga tgctgccgtc gggcgcgggt ataatttgca ggctgtcgag
     6481 caggattttc ggctgtttgc cgtaattttc atccacataa atgtcaaacc agccgtccga
     6541 gtgcgtatcg ggcagcggcg gcagtttggc ggtatgttct ttaaattcgc gggcggcggc
     6601 ttcttcgggc gtttcgcggt agcgggtgtt gatcggcgtg tctttttggc tgaagctggt
     6661 ggcaagggac gtgccggcgg cgagtgcgag cagcaggagg gcggtgcggc gcataagttt
     6721 ctccaaattg aaaacggcgt tattttatgg gttggcaaag ggggctgcaa gcaactgggg
     6781 tataatctcc ccccgattcc cattttttaa acggtacaaa cgatgaacag cgaaacttta
     6841 gacgtaaccg gattgaaatg tcccctgccg attttgcggg cgaaaaaggc tttggcgcaa
     6901 atgcagcagg gcgacgtgtt gaccgttctg gcaaccgacg gcggcgtacc gggggatttt
     6961 gaggcttttt gccgccaaac cggtcatgtg ctgttggatg cttccgaaca ggacggcgtg
     7021 ttcacgctgg tcgtccaaca caaataaatg ccgtctgaaa tgcggatgtc ccgcccgacg
     7081 gcgttgtttt tgaatatcgt atgtgccgcg tgccgtttca aaaacaaacc gtccggcgtg
     7141 cagtgtcggc atttcgggcg tatcgccgcc gatttgttcc ggaatggctg ttaaccgcct
     7201 tgccgtcggc aaagaagcaa aacccaagca caatcaaaat ctaaaggctg tgtttgaaga
     7261 ttccgttgat gcaacccaat cagtcggaca aaatgccttt catccaatgg aaccggtttc
     7321 cgaccggacg gaataaccgg ctttcccccg gcaaacggat ggaatcgacc gggtattcaa
     7381 acgcagccaa aacctaaaaa ggaacaacca tgcaaaccct gaccattatc cgccccgacg
     7441 atatgcacct gcacctgcgc gacggcgacg cgctcaaagc cgttgctcct tataccgccc
     7501 gccaaatggg gcgcgccgtc atcatgccca acctcaaacc gcccgtcgtc agcgtagccg
     7561 atgcgcttgc ctacaaagcg cgtattatgg cggcgttgcc cgaaggcagc gcgtttgagc
     7621 cgctgatgac gctttatttg accgacaacg ccacgcctgc acttgtgcgc gaagccaaag
     7681 ccgccggcat cgtcgccttc aaactctacc ctgccggcgc gaccaccaat tccgattccg
     7741 gcgtaaccga cctgttcaag ctcatccccg tgttggaaga aatggcgaaa cagggcattt
     7801 tgttcctcgt tcacggcgaa gtaaccgacc ccgaaatcga catcttcgac cgcgaagccg
     7861 cctttatcgg gcgcgtgatg aaacccgttt tggcgcaagt gccgaatctt aaagtcgtgt
     7921 tcgaacacat caccaccgcc gaagccgccc gcctggtttt ggaagcaggc gacaacgtag
     7981 ccgccaccgt taccccgcaa cacctcctgc tcaaccgcaa cgacctcttg gtcggcggcg
     8041 tgcgccccca tcatttctgc ctgcccgtac tcaaacgcga aacccaccgt caggcattgg
     8101 ttgccgccgt taccggcgag aaggcgcata aattcttcct cggcaccgac tccgcgccgc
     8161 acgccaaatc cgccaaagaa aacgcctgcg gttgcgccgg tatgttcagc gcgatgaccg
     8221 ccatcgagct ttatgccgaa gtgtttgaaa aagcaggcgc gttggacaaa ctcgaagcct
     8281 tcgcctcgaa aaacggcgca aggttttacg gcattcctga aaataccgac acgatcaccc
     8341 tcgtcaaaca aagccaaacc gtccccgcaa gtattcccta cggcgacggc gaacttgtcc
     8401 cgatgcgcgc gggcggcgaa atcggctgga cggtacagta ttgatggggc tggaaacaaa
     8461 atgccgtctg aaaggttttt cagacggcat ttgtgttgtt gactgattga tacgtcaacg
     8521 gcgtttgagt ttgttacgtt tcggttattt ccgataaatt cccacaattt tcaaatttcg
     8581 ccattcccac gaaggcagga atccggaaat tcgatgcgac cagagtttat caaaaacggc
     8641 agcaactcaa aaaaccggat tcccgcctgc gcgggaatga cgagattgaa gtttcagaat
     8701 ttatttgaaa tacccaaaat tcaaaaaacc aaattcccac ctgcgtggga atgacgaaac
     8761 aaagaaagca gaaataagga catagaactt tctttaaatt tgtgatgcat caacggcgtt
     8821 tgggctcgtc gggacggatt tgggcggcga gtttgtcgag gatgccgttg acgaatttgt
     8881 gcccgtccgt gccgccgaag gttttggtta cttcgatggc ttcgttgatg atgacggggt
     8941 agggcgtttc gggcatggcg gacagctcgt ggcaggcggt cagcaggacg gcgcgttcga
     9001 tggggttgag gtctttttcg tcgcggtcga gcagggggcg gatttgtcgg atgtactctg
     9061 ccgcattggt ttgcgtgccg aagaaaagtt tgttgaacaa ttcttcgtct gccttggcaa
     9121 agtcgggcat ttcgcggatg tttttagcaa tctcgggcgc ggcggtgcgg ttgataaggg
     9181 attggtaaac ggcttgtacg gcaagctcgc gggaacggcg gcgggctgtt ttcatgattt
     9241 ttccttgaaa cggttgggcg gcacggtatg ccgtctgaaa cggaaagggc gtatcggtgt
     9301 acgccctgtt tgttattctt cgtcttcaaa ctgttcttcg agcaggaggt tgacgaggtt
     9361 ggcgcattct acggcgactt tggcggcatc cgaggctttt tcttcaatcc gttcgattgc
     9421 ctgtgcgtcg ttttccgtgg tcaggacggc attggcaatc gggatgttgt agtcgagtgc
     9481 gacgcggctg acccctgctc cggattcgtt ggaaaccagc tcgaaatggt aggtttcgcc
     9541 acggataacg acgccgatgg caatcagtgc gtcaaatttt tcagaagagg caaagttcat
     9601 cagcgcgatg gggatttcaa gcgcgccggg tacggtggcg acggtaatgt tttcgtctgc
     9661 cacgcccaat tcttggaggg tgcggcagca gactttgagc atttcgctgc cgatttcgtt
     9721 ggtgaagcgt gcctgtacga tgccgatgcg gaggtgtttg ccgtcgaggt tgggggcgat
     9781 ggtgttcatt gggtgtcctt tggtattcgg aggtttcgga atgccgtctg aaggttttag
     9841 tcttgcggct gccagtcggc gacggtttgg aatgtgccgt cttctgccaa ttcccacgcg
     9901 ctgccttcag gttgggagag cagtgcggcg gtttcagggt tggttttggt gatatcggcg
     9961 aggctgacga tgctgaagtt gtccgggtcg tcggtgtatt cgtcggtctc gtcgccgctg
    10021 aagaaacgcc agccgctgtc gttttcaaaa acgggggctt cgcggtagag gaagccgacg
    10081 ggctggtttt gtttggcgac ggtgttggtg gcgatgcagc ggtcgagtgc cgaggaaagt
    10141 gcttgtgcaa atgcgttcat tgcgggaata cgttggggga aaacttgcgg attttaccac
    10201 gattcttgcg ttgtcggcag acggcggcgg tttggtggta caatgtgcgc cgtttgcagc
    10261 cttaaggtgt ttctgtattt ttggagtatg gaaacgcatt cgggctgttt tttgcggaag
    10321 acggtaatga aagacgatgt tttgaaacag caggcacacg cggcgataca gaagagactg
    10381 ggctacgcgt tccgcgatat ttcgcttttg cggcaggctt tgacgcacag gagccatcat
    10441 gcgaagcaca acgagcggtt cgagtttgtc ggtgattcga ttttgaatta tacggtggcg
    10501 cggatgctgt ttgacacgtt tccgaagttg accgagggcg agttgtcgcg gttgcgggca
    10561 agtctggtca atgagggcgt gctggcggaa atggcggcgg aaatgaatgt cggcgacggt
    10621 ctgtacttgg gggcgggcga gttgaagagc ggcggcttca gacggccttc gatactggca
    10681 gacgcgatgg aggcgatgtt tgcggcggtc agcttcgatg ccgatttcaa cacggcggaa
    10741 aaggtggtgc gccatttgtt tgccgaccgc gtccggtgtg ccgatttcca aaatcaggca
    10801 aaagacggca aaactgcttt gcaggaggcg ttgcaggcac gccgctttgc tttgccgaaa
    10861 taccgtatcg aagagcaaat cggccatgcc aacgacagta tgtttgtggt ttcctgcgat
    10921 ttgggcgaac tgggtttcgt gtgccgtgcc aaagggacga gccgcaaggc ggcggaacag
    10981 gaggcggcga aagaggcttt gaaatggctg gaagagaagc tgccgctgaa gaagaaaaag
    11041 aaatgaggcg gcgcgtgaat atgccgtctg aacatatgga tacgaaagca aatatggata
    11101 ttgaaacctt ccttgcaggg gaacgcgccg ccggcggata ccgttgcggc ttcgtggcga
    11161 ttgtcggccg tccgaacgtg ggcaaatcaa cgctgatgaa ccatctcatc ggtcagaaga
    11221 tcagcatcac cagcaaaaag gcgcagacga cgcgcaaccg cgtaacgggg atttataccg
    11281 acgataccgc gcagttcgtg tttgtcgata cgcccggctt tcaaaccgac caccgcaacg
    11341 cgctcaacga caggctgaat caaaatgtta ccgaggcgct cggcggcgtg gatgtggtgg
    11401 ttttcgtcgt ggaggcgatg cgctttaccg atgccgaccg cgtcgtgttg aaacaactgc
    11461 ccaagcacac gccggtcatt ttagtggtca acaaaatcga caaggacaag gcgaaagacc
    11521 gttacgcgct ggaggcgttt gttgcccaag tgcgcgccga atttgaattt gcggcggcgg
    11581 aggcggtcag cgcgaaacac ggattgcgga ttgccaacct gttggag
//
LOCUS       DAWHXZ010000043        11579 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000043 DAWHXZ010000000
VERSION     DAWHXZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11579
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.043"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            306..1460
                     /gene="obgE"
                     /locus_tag="WIE35_000749"
     CDS             306..1460
                     /gene="obgE"
                     /locus_tag="WIE35_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ4153695.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTG
                     ENVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1543..2592
                     /locus_tag="WIE35_000750"
     CDS             1543..2592
                     /locus_tag="WIE35_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ4153696.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3280..4701
                     /locus_tag="WIE35_000751"
     CDS             3280..4701
                     /locus_tag="WIE35_000751"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ4153697.1"
                     /translation="MTTIYNTLTRQKELFAPIDPKNVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPTEFLQGSAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4872..5651
                     /gene="xth"
                     /locus_tag="WIE35_000752"
     CDS             4872..5651
                     /gene="xth"
                     /locus_tag="WIE35_000752"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ4153698.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            5638..5982
                     /locus_tag="WIE35_000753"
     CDS             5638..5982
                     /locus_tag="WIE35_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153699.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIASKCLGWKGWRLLANVLACLAVRTLLSVPAT
                     LLIAFTLQDLLK"
     gene            5992..6522
                     /locus_tag="WIE35_000754"
     CDS             5992..6522
                     /locus_tag="WIE35_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ4153700.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHAFQRVRCPCRAALVVPEHGGVPHGWAQRQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6615..7730)
                     /gene="asd"
                     /locus_tag="WIE35_000755"
     CDS             complement(6615..7730)
                     /gene="asd"
                     /locus_tag="WIE35_000755"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ4153701.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMQEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKTNF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLEMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            7907..8488
                     /locus_tag="WIE35_000756"
     CDS             7907..8488
                     /locus_tag="WIE35_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ4153702.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(8550..9404)
                     /gene="folD"
                     /locus_tag="WIE35_000757"
     CDS             complement(8550..9404)
                     /gene="folD"
                     /locus_tag="WIE35_000757"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ4153703.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            9627..9704
                     /locus_tag="WIE35_000758"
     tRNA            9627..9704
                     /locus_tag="WIE35_000758"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9662..9664,aa:Pro,seq:tgg)
     gene            9729..9805
                     /locus_tag="WIE35_000759"
     tRNA            9729..9805
                     /locus_tag="WIE35_000759"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9763..9765,aa:Arg,seq:tct)
     gene            9833..9908
                     /locus_tag="WIE35_000760"
     tRNA            9833..9908
                     /locus_tag="WIE35_000760"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9866..9868,aa:His,seq:gtg)
     gene            10044..10550
                     /locus_tag="WIE35_000761"
     CDS             10044..10550
                     /locus_tag="WIE35_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ4153704.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            10547..>11579
                     /locus_tag="WIE35_000762"
     CDS             10547..>11579
                     /locus_tag="WIE35_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ4153705.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLD"
BASE COUNT         2811 a         2889 c         3147 g         2732 t
ORIGIN      
        1 cgagtttatc ctagttatct aggacagccc caaaacttat tatatgtaac ggtttgaacg
       61 gacattccgt cgggtcggaa tgtcaaaaag gcgcgattgt accaaagaag ttggacataa
      121 ttttgtttgc aggctgaaga tttgcttaaa aattcattaa gatgggcgga acaaatagtt
      181 tgggtacggc gtgttgaaat accgcttatc ccttataata gcgtccgaaa ttctgttcag
      241 acggcatcaa aacacacggt aatccgtctg aacccccatt tgaaaccaac aaacaagagc
      301 attgaatgaa attcatcgac gaagcaaaaa tcgaagtcgc cgcaggcaaa ggcggtaatg
      361 gcgcaaccag tttccgccgc gaaaaattcg taccgcgcgg cggtccggac ggcggcgacg
      421 gcggcaaagg cggcagcgtt tgggcagaag ccgacgaaaa caccaacacc ctcgtcgaat
      481 accgcttcgt taaacgctac caagccaaaa acggcgaaaa aggccacggt tccgaccgtt
      541 acggcgcagg tgcggacgac atcgtcctca aaatgcccgt cggcaccctt atccgcgacc
      601 tcgacaccgg cgaaaacgtt gccgacctca cttatcacgg acagcgcgtc tgccttgcca
      661 aaggcggcaa aggcggcttg ggcaacatcc acttcaaatc ctccgtcaac cgcgccccaa
      721 aacaatccac gcccggcgaa gaaggcgaag cccgttccct gcaactcgaa ctcaaagtcc
      781 tcgccgatgt cggcttattg ggtatgccca acgccggcaa atccaccctg attaccgccg
      841 tatccgccgc acgccccaaa atcgccaact accccttcac caccctgcat ccaaacttag
      901 gcgtggtacg catcgacgaa aaccacagct tcgtgatggc cgacatcccc ggcctgattg
      961 aaggcgcggc agaaggcgcg ggtttggggc atcgtttcct caaacactta tcgcgcaccg
     1021 gattattgct gcacgtcgtc gatttggcgc ccttcgacga aaccgtcaac ccggccgaag
     1081 aagcacttgc tatcatcaac gaattgcgca aatacgatga agaactttac ggcaaaccgc
     1141 gctggctggt gctgaataaa ctcgacatgc ttgacgaaga agaagcccaa acgcgcactg
     1201 ccgccttcct cgaagccgtc ggctgggact accccaaacc cgacgaccgt ttccaatttg
     1261 acatggaaac cccgcgcctc ttccaaatca gcgcgctgac ccaccaaggc acgcaggaat
     1321 tggtacacca aatcaaccaa tacctgaccg agaaaaaacg tatcgaagct gaaaaagccg
     1381 aagcggagaa agcagcggca aatgttgaaa ttatcgaaca gcagcctaaa acggatacgg
     1441 gcgtgtttaa gccggagtga aaaaagaaag ggtttcagac gatatttgtt ctacaaaagg
     1501 ttgtttgaaa caattttaaa gcaagattga aatggattaa aaatgtcatc taaccaatat
     1561 atccagtcgc ttgagttgac aaactttacc gttcttccaa acgatacttt tgaattttct
     1621 gaaaatctca atgttattgt tgccgaaaac ggttgtggca aaacacattt gttaaaaatt
     1681 ttatacagcc tgctagaagt aacatctaac accaaaaaca gattactgaa aacagaattg
     1741 caaaaaagtt ttgccgataa attactgaat gttttccgtc ccgattcctt gggcaggttg
     1801 agcaaacgtt tgcaggggcg cgggcgtacg gaaatcgttt taaaactgca aaacggcact
     1861 acgcatagta gtctgaattt ttccagtaac tcatcatcgc aagtcaatgt tcaaagcatc
     1921 ggattgaaag aaaatgaata tactccgaca cctatcttct tacccagccg cgaattaatt
     1981 acactttgcc cttggttcac ttctttgtat caaaaccaaa gcatcccttt cgaagaaact
     2041 tggtttgata cctgtatgca attaaatcac ccgcttgcca aaggccccag agaaacaaaa
     2101 attcgagaat tgctcgaacc tattgagaac gcgatgggcg gaaaagtatc tgaagaacaa
     2161 ggaaggtttt atctatcgct gacaaatacg ggcggtaaga ttgaagcacc tttggtagca
     2221 gagggtttgc ggaaatttgt catgattgcc cggttaatcg caacaggggc attgctggat
     2281 aaaggctatc tattttggga tgaaccggaa gctaatctga atcctaaatt gatcaaagtg
     2341 gctgcccgta ttatttggtc tttatctcaa cagggtattc aggtttttat tgcaacacac
     2401 tctttattct tattgagaga attggaactt ttgaagcttg aaaataacac ggatttacct
     2461 gtacgttttt tctcgttgat tgcttccgat gatggaacaa aagtggagca agggaacagt
     2521 attaatgatt tgaacacatt gatattgtta gatgaaaaca ttatgcaggc agacaggtat
     2581 ttgaatattt gaggctaagc tatgactaca atcacagaaa agagattaac ctttgctttt
     2641 cctgaagatt attacgttac caagtatgac gaatgggaac attataaaat ttttcagaac
     2701 agttgtaatt tacgtaataa aattgataca aatgaaaagg gaaagaacgg gataaatcaa
     2761 agtgtagatg acgataatgg cagctcaggt gtagatataa tagcccttca tgaatcgaca
     2821 ctttggctaa tcgaaattaa agattattac cgacttgggc ttgagccaaa tgcccaatca
     2881 atagatgaaa aactttctga cctaccatat ttaatcgcaa gaaaaattcg agattcattg
     2941 gcaggattgg tttctgctaa atttaaagca gaaaaacagg aagaaaaaga tttttctcgc
     3001 ttggctttag attgtaacga aataaaaata gtccttcata ttgaaatgcc tagtataaga
     3061 agtaaacttt atccctcgtc atcagattta gcaaacttat taaaagataa gtttaaatta
     3121 tcagaattta caaaaaattt tgcaaactgt tatgcagaac caattttcac aaatatcagc
     3181 catattaata atccgcaact gcgtaatgtt ccttggtccg tatctacagg aacagaacag
     3241 aaattatcat ccgagcaaca aagattaatc cataatccca tgaccaccat ctacaacacc
     3301 cttacccgtc aaaaagaact cttcgccccc atcgatccga aaaacgtgcg tatgtacgtt
     3361 tgcggcatga ccgtttacga ctattgccac ttaggccatg cccgtgtgat ggtggtgttt
     3421 gacatgattg cccgctggtt gcgcgagtgc ggctatccgc tcacttatgt acgcaacatc
     3481 accgacatcg acgataaaat cattgcccgt gcggctgaaa acggcgagac tatcggcgaa
     3541 ctgaccgcgc gtttcattca ggctatgcac gaagatgccg atgctttggg cgtgttgcgt
     3601 ccggatattg agccgaaggc aacggaaaac attccgcaaa tgattgccat gattgaaacc
     3661 ctgattcaaa acggcaaggc atatcctgcc gcaaatggcg acgtttacta cgccgtgcgc
     3721 gagtttgccg cttacggaca actgtcgggc aaatcgttgg acgacctgcg cgcgggcgaa
     3781 cgcgtggaag tggacggttt caaacgcgat ccgcttgatt ttgtattgtg gaaagcggcg
     3841 aaagcaggcg aaccggcgtg ggaaagcccg tggggcaacg gccgtccggg ttggcacatt
     3901 gaatgctctg ccatgagtga aaacctgttc ggcgatacct tcgacatcca cggcggcggc
     3961 gcggatttgc agttccctca tcatgaaaat gaaatcgccc aaagcgtcgg cgcgacgggg
     4021 catacctgcg gtcatcatca cgcgcaaacc caccacgggc aaagcatcgc cagccacgtc
     4081 aaatattggc tgcacaacgg ctttatccgc gtggacggcg aaaaaatgtc caaatcactg
     4141 ggcaacttct tcaccatccg cgaagtgttg aaacaatacg accccgaagt cgtgcgcttc
     4201 ttcatcctgc gcgcccacta ccgcagcccg ttgaactatt ccgacgcgca tttggacgat
     4261 gcaaaaggtg cgctgacccg tctgtacacc accttgaaaa acacgccggc ggctgagttt
     4321 gatttatccg aaaacgccaa cggctacacc cgccgcttct acgccgccat gaacgacgat
     4381 ttcggtacgg tcgaagcggt tgccgtgttg ttcgaactgg caggcgaagt gaacaaaacc
     4441 aatgacgcac agctcgccgg ctgcctgaaa gccttgggcg gcatcatcgg cctgctgcaa
     4501 cgcgacccga ccgagttcct gcaaggcagc gcggcttcag acggcctctc caacgaagaa
     4561 atcgaagact tgatcgcccg gcggaaacag gcgcgcgccg ataaaaactg ggcggaatcc
     4621 gaccgcatcc gcgaccttct gaacgaacac aaaatcattt tggaagacaa cgccggcggc
     4681 acgacttggc ggcgcggttg aactgtatcg gaaagggcaa aagtaccgcc ttttgctctt
     4741 tttccgtttt cagacggcat ttcccgcttg aagccccgcc gttttcagca ggcagggttg
     4801 aatgggaaat gccgggcggc gcaccaggtg ccgttttgtg tatgggaaca ttgtgagaac
     4861 ttatttttat tatgcttaaa atcatttccg ccaacgtcaa cggcatccgc tccgcctaca
     4921 aaaaaggttt ttacgaatac atcgccgcat cgggcgcgga cattgtctgc gtgcaggaac
     4981 tcaaagcgca ggaggctgat ttgtctgccg atatgaaaaa tccgcacggg atgcacggcc
     5041 attggcattg tgccgagaag cgcggttaca gcggcgtggc ggtgtacagc aaacgcaaac
     5101 ccgacaatgt gcaaatcggt atgggcattg aagaattcga ccgcgaaggg cggtttgtgc
     5161 gttgcgattt cggcaggttg agcgttattt cgctttactt gcccagcggc agcagcgcgg
     5221 aagaacgcca gcaggtgaaa taccgtttcc tcgatgcgtt ttaccctatg ctcgaagcga
     5281 tgaaaaacga agggcgcgac attgtcgttt gcggtgactg gaacatcgcc caccaaaaca
     5341 tcgacctgaa aaactggaaa ggcaaccaga aaaattcagg tttcctgcct gaagagcgcg
     5401 agtggatagg caaggttatc cacaagctcg gttggacgga tatgtggcgc acgctttatc
     5461 ccgatgtgcc gggctacact tggtggagca accgagggca ggcgtatgcg aaagatgtcg
     5521 ggtggcgcat cgattatcag atggttacgc ccgaacttgc cgccaaagcc gtgtccgcac
     5581 acgtttataa agatgaaaaa ttttccgacc atgcgccgct ggtcgtggag tatgactatg
     5641 ctgccgaata aagttttggg taaatatgat tggaatgtgg acggaaaaac aggaataggc
     5701 gcggcatggg ttgtcgctgc gtttatcctg cccatattgg tgtgggcgat atttatgttg
     5761 tcgcggatgc aaggctggct tgcgccgacc aaggcaaacc cgatatgggc gttggtgtgg
     5821 ctgctgattt gcctgccctg cctgctgatt gcgtccaaat gtttgggttg gaaaggctgg
     5881 cggctgttgg caaacgttct tgcctgcctc gccgtgcgca ccctgttgag tgtgcctgcc
     5941 acgctgctga ttgcgttcac gctgcaagac ctgctcaaat aggcgcggac aatggaaagt
     6001 ttcgagcttg aggatttgac gctgtggctg gtacgcgatg cggatgaagc ggaaatgtgg
     6061 atagaccgct gggcgatcag ttatcccgtc gtgcagatgt ccgaagcgtc ggccggtcaa
     6121 agcatagggg aatggcaggc agggcttcag acggcatttg aacgcatacg cggcaaatac
     6181 gtcgccgttg tcgcacacgg tgcgggcgcg gccgcatttt tggcgtggct gtatcaggca
     6241 gacatcctga cacggaagaa aattgccaac atcatccttg taccgcagcg tcccgatatt
     6301 tttcccgacg atgcggaaca cgctttccaa cgcgtccgct gtccctgccg tgccgcattg
     6361 gttgtacccg aacacggcgg cgtgccgcac ggttgggcgc aaagacaggc ggatttgtgg
     6421 aacgcccgcc tgttggtttc cccgcattcg ggcagtttga acggtatgct cggcggctgg
     6481 cagtggggta tgaagctgat gcaggaaatg ttgctggcgt gaacgcttgc cttatcggaa
     6541 tttcggttaa ctggggttgc aaggaaaatg ccgtctgaaa caggcttcag acggcatttc
     6601 cattcaaaca gggcttacag gctgcccaac acgatacgca gcacgcggcg cagcggctcg
     6661 gccgcgcccc acaagagctg gtcgccgaca gtgaacgcgc tgatgtattc gccacccatc
     6721 tccagtttgc ggatgcgtcc gacagggacg gacagcgtgc cggtaacttt ggcaggagtc
     6781 agctcgtgga tgctggcttc tttttcattg gggatgactt tcacccagtc attcgcgcct
     6841 gccaaaatcg tttcgatttc ggaaacaggc aggtcttttt tcaacttcag agtgatggct
     6901 tggctgtggc agcgcatcgc gccgacgcgg acgcacaggc cgtcaatcac ggttggattg
     6961 tcgctgcggc cgaggatttt gttggtttcc acgccgcctt tccattcttc tttggactgg
     7021 ccgttgccca aatccacgtc aatccacgga atcaggctgc cggcgagcgg tacgccgaag
     7081 ttggttttcg gatagtcttc gctgcgcagg aaatcggata ctttgcggtc gatgtcgaga
     7141 atcgagccgg caggatcggc aagctcgtcc gccacttggg cgtgaaccgc gcccataccg
     7201 ctgatgagtt cgcgcatatt tttcgcgccc gcgccggaag cggcctgata ggtcatactg
     7261 gtcgcccatt cgaccaaatc gttttggaac aggccgccca aagccatcaa catcagggag
     7321 acggtgcagt tgccgccgat gtagtttttc acgccgtttt tcaaaccgtt gtcgatgacg
     7381 ttgcggttga cggggtcgag gacgataatc gcgtcgtctt tcatacgcag cgaggatgcc
     7441 gcgtcaatcc agtaaccgtt ccagccgctg tcgcgcaggg cttggaagac ggatttggtg
     7501 taatcgccgc cttggcaggt aacgatgatg tccatttttg ccagttcggc aacgttgttc
     7561 gcgtccaata atgttttagc cgcctgaccg aaatcagggg ctgcgccgcc gacgttggaa
     7621 gtggtaaaga aaaacgcttc gggaatgtgg gcgaagtcgt tttcttcttg catacgctgc
     7681 atcaaaaccg aaccgaccat gccgcgccag ccgacgaaac ctactttcat tgtgttgctc
     7741 cttgaggaaa aaatcgttaa caaaattatg tatggcgtaa aggaagcgtt ttaacgccgt
     7801 attgggaaaa tgtaaagttc tttatcggga tttctttaag tattggcgtt ttcggtgtcg
     7861 tggcggtaga atcggggtca gttaagaaca attatcagga acggatatgg aagaaaaaaa
     7921 cgactacacc gacgcggcat ccgacaaccg caacgggcag gaaattgaag tcggaattgc
     7981 cggcgcaggc gaccggatac ttgccgcgct gctgaaccaa ttgtttacct ttttggtttt
     8041 gttggtgccg tttgccggac tgattgcttt cgccatcaag aatgaaggca ggattggcgg
     8101 cagtgaggaa atattcgggc tgcttttggg catgacttcc ttttgggtag gactggcggg
     8161 cattttggtt tataccgtcg ttcaaatcta ctatatgagt cgggacgggc agtcattggg
     8221 taagaagatc atgagaatca gggtgttgaa aaccgacggc cgcaatccgg gttttgtcgg
     8281 cacggttttg gtacgcgaaa tcgcatggtc ggttttggtt gccattattg ccgccgttat
     8341 cggtcttgca gtaggtgaca acggagaaaa cgccatcaac ctgctggcat tccttgccaa
     8401 ctttgtcctg ctctttatgg tcaaacgcga ccgccgcacg ctttacgaca tactggcgga
     8461 tacggttgtc gtcaagctgc ccaaataaac ggacacggca aaatgccgtc tgaaagcctt
     8521 tcagacggca tttttatctt cagaaccgct caagcatcgt gcagtgaagc cgcgtgcagg
     8581 gtgttttcca tcaatgtggc aatcgtcatc ggacccacgc cgccgggaac gggcgtaatc
     8641 atcgccgccc gttcttttgc cgtttcaaat tccacgtcgc cgcacaggct gccgtcgtcc
     8701 aaacggttga tgcccacatc gataacgacc gcgccgggtt tgatccattc gcctttgaca
     8761 aagttcggaa tgcctacgcc gaccaccaaa atatcggctc cggcaacctc gtctgtcaga
     8821 ttttcggttg cgctgtggca gaccgttacc gttgcgcgcg ccagcagcag ttccaaagcc
     8881 tgcgggcggc cgacgatatt cgacgcgccg accacgaccg cttttttccc cttcggatca
     8941 atgccgtaag cttccaaaag cgtcatcacg cccttgggcg tacacgggcg catcagcggc
     9001 attttgaccg ccagcctgcc gacattgtaa ggatggaagc cgtccacgtc cttatccggc
     9061 gaaatacgtt ccaaaaccgc ctggctgtcg aggtgcttcg gcagcggtag ctgaaccaga
     9121 ataccgtcca cttcggaatc ggcattcagg cggtcgacca gtgccagcag ttcttcctgc
     9181 gatgttgatt cgggcagctc gtaagacagt gatttgatgc cgcatttttg gcaggcagtt
     9241 ttcttgttgc ggacataaac cgcgctggca gggtcgcctc cgaccaaaac cacggccagg
     9301 caaggcatgt gcagattgtc ctgttggcgt tgcgccaccg cttcggcaac cgcctgcagg
     9361 cgtttttgcg aaacttcttt accattgatc agttgtgccg acatatccgt ccttttgtta
     9421 aacaggtaga aaatgctgaa aattatcgca ttttccgttt gtttttacag caggatgccg
     9481 aaaataaatt tgccctgaaa taaaatacgg ctgtttggac ggattaaata ttttttcaaa
     9541 tcaaaaaact atatttttca tcttggtgtc ttgaaaataa atattgacgc atctttccct
     9601 gagttgtata gttcgcttct tcaagtcggg gcgtagcgca gcccggttag cgcatctgct
     9661 ttgggagcag agggtcgtga gttcgaatcc caccgccccg accaatcaat cagcacgaat
     9721 cggcatgagc gcccgtagct caaccggata gagcaccgac cttctaagtc gggggttaca
     9781 ggttcgattc ctgtcgggcg tgccaagaat ttgattgaaa accgttttta tggtggctgt
     9841 agctcagttg gtagagcccc ggattgtgat tccggttgtc gtgggttcga gccccatcag
     9901 ccaccccaga taaaggcccg tacttttcgg tacgggtctt ttcccgtttg cgcgtactgt
     9961 ccgcgccgcc ggctttgcct ttcgcatatg cttctgtatc atgtgggttt ttctgtcttt
    10021 tatggttttt gtcggaggct ttgatggttg acgcttggtc tgtccctgat tttgaatcga
    10081 aaatctgtcc gcccgaggcg ttggcggcgc gtttggcgtt gttgccgcgc ccgctggtgt
    10141 ttaccaacgg ctgtttcgac atcctccaca gggggcacgt tacttatctg gcgcaggcgc
    10201 gttcgatggg gggcgcgttg gtgctggcgt tgaataccga tgcttcggtg cggcgtttgg
    10261 gcaagggcgg cgaccgcccg attaatcctt tggagaaccg tgccgccgtt gccgccgctt
    10321 tggaaagtgt ggatttggta acgtggtttg acggggatac gccggcggcg ttgattgagg
    10381 cggtcaaacc tgagattttg gtcaagggcg gcgattgggc tgcggataag attgtcggtg
    10441 cggcagaaac gttggcgcgc ggcggtcagg tgttttcaat tccgtttctg caccagactt
    10501 cgacaacgaa gactttggca aaaatccgtg cggcagaggg cggaaaatga cggttttgaa
    10561 gctttcgcac tggcgggtgt tggcggagct tgccgacggt ttgccgcaac acgtctcgca
    10621 actggcgcgt atggcggata tgaagccgca gcagctcaac ggtttttggc agcagatgcc
    10681 ggcgcacata cgcgggctgt tgcgccaaca cgacggctat tggcggctgg tgcgcccatt
    10741 ggcggttttc gatgccgaag gtttgcgcga gctgggggaa aggtcgggtt ttcagacggc
    10801 attgaagcac gagtgcgcgt ccagcaacga cgagatactg gaattggcgc ggattgcgcc
    10861 ggacaaggcg cacaaaacca tatgtgtgac ccacctgcaa agtaagggca gggggcggca
    10921 ggggcggaag tggtcgcacc gtttgggcga gtgtctgatg ttcagttttg gctgggtgtt
    10981 tgaccggccg cagtatgagt tgggttcgct gtcgcctgtt gcggcagtgg cgtgtcggcg
    11041 cgccttgtcg cgtttaggtt tggatgtgca gattaagtgg cccaatgatt tggttgtcgg
    11101 acgcgacaaa ttgggcggca ttctgattga aacggtcagg acgggcggca aaacggttgc
    11161 cgtggtcggt atcggcatca attttgtcct gcccaaggaa gtagaaaatg ccgcttccgt
    11221 gcaatcgctg tttcagacgg catcgcggcg gggcaatgcc gatgccgccg tgctgctgga
    11281 aacgctgttg gtggaactgg acgcggtgtt gttgcaatat gcgcgggacg gatttgcgcc
    11341 ttttgtggcg gaatatcagg ctgccaaccg cgaccacggc aaggcggtat tgctgttgcg
    11401 cgacggcgaa accgtgttcg aaggcacggt taaaggcgtg gacggacaag gcgttttgca
    11461 cttggaaacg gcagagggca aacagacggt cgtcagcggc gaaatcagcc tgcggtccga
    11521 cgacaggccg gtttccgtgc cgaagcggcg ggattcggaa cgttttctgc tgttggacg
//
LOCUS       DAWHXZ010000044        11502 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000044 DAWHXZ010000000
VERSION     DAWHXZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11502
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.044"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            499..1800
                     /gene="bioA"
                     /locus_tag="WIE35_000763"
     CDS             499..1800
                     /gene="bioA"
                     /locus_tag="WIE35_000763"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ4153706.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLRAKQNMATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNLTSRFDDEWDEADLQPVRALFEAHHTDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKCRLKTAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            1797..2444
                     /gene="bioD"
                     /locus_tag="WIE35_000764"
     CDS             1797..2444
                     /gene="bioD"
                     /locus_tag="WIE35_000764"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ4153707.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLYSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            2411..2641
                     /locus_tag="WIE35_000765"
     CDS             2411..2641
                     /locus_tag="WIE35_000765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153708.1"
                     /translation="MDGVGKNRRGIKIGKNMEHLYDEYGNTTPNRRTTNKKDETISSK
                     VTVAAIPVAAPFAIADIVDQDFIQAIMKITGH"
     gene            2665..3051
                     /locus_tag="WIE35_000766"
     CDS             2665..3051
                     /locus_tag="WIE35_000766"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153709.1"
                     /translation="MKCYIKVVIIVFIMVMTTSCNPCFFATSSCDYSFRKNYPINSDD
                     EYYVKNNTSKETRIADLRYCISYACPEVYQKYQLPDENGDYREIIRRKYKDDYKKGYK
                     NADDVMTCYGKIGECMKNNKGYSTKW"
     gene            3045..3488
                     /locus_tag="WIE35_000767"
     CDS             3045..3488
                     /locus_tag="WIE35_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153710.1"
                     /translation="MVMFRLLSTALVLLIMQGCYYTTIDNEKCFVAPQSVHCPVSSDI
                     ENVIRKNPNKSINQVRSDIKYCANMYFSNIRELNIPQDFDNYIFMLKEKGIPRNDKEL
                     AYNAVRNMHIICLSSLGGYDYYQKTAEFQKIDYKKEMEKLLNENH"
     gene            3670..3942
                     /locus_tag="WIE35_000768"
     CDS             3670..3942
                     /locus_tag="WIE35_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153711.1"
                     /translation="MTIRANNDIEYFVKNNTTNEQRIDDFRSCLSVACPEIYKLYQLP
                     DEKGNYQYIIKKYYDNKYNDNAVKSTNCIGDNARCMLNNKGYSTKW"
     gene            3945..4421
                     /locus_tag="WIE35_000769"
     CDS             3945..4421
                     /locus_tag="WIE35_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ4153712.1"
                     /translation="MGYGFEEWLPDLPAPVSDGIGLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            4451..5341
                     /gene="ubiA"
                     /locus_tag="WIE35_000770"
     CDS             4451..5341
                     /gene="ubiA"
                     /locus_tag="WIE35_000770"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ4153713.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVGKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            5526..5975
                     /gene="ptsN"
                     /locus_tag="WIE35_000771"
     CDS             5526..5975
                     /gene="ptsN"
                     /locus_tag="WIE35_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ4153714.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            5977..6939
                     /locus_tag="WIE35_000772"
     CDS             5977..6939
                     /locus_tag="WIE35_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ4153715.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            6920..7774
                     /gene="rapZ"
                     /locus_tag="WIE35_000773"
     CDS             6920..7774
                     /gene="rapZ"
                     /locus_tag="WIE35_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ4153716.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLPSR"
     gene            7855..8796
                     /gene="ylqF"
                     /locus_tag="WIE35_000774"
     CDS             7855..8796
                     /gene="ylqF"
                     /locus_tag="WIE35_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ4153717.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGKKPSEA"
     gene            8897..9499
                     /locus_tag="WIE35_000775"
     CDS             8897..9499
                     /locus_tag="WIE35_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ4153718.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            9683..11356
                     /gene="recN"
                     /locus_tag="WIE35_000776"
     CDS             9683..11356
                     /gene="recN"
                     /locus_tag="WIE35_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ4153719.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
BASE COUNT         2980 a         2701 c         2999 g         2822 t
ORIGIN      
        1 ggctgtaacc ttcaatcgga atttgttgcc tgcgggatat gcggtatgaa tgtttgggat
       61 atatggggca ggatggtgga aatctatcat aagtataaga agccgtgcct ggttttggcg
      121 gtggattttg tgatgggtat ggtattcata gagccgaatg aggagctgtg catcggtaga
      181 tgctatgcgc ctatgtcgga gtcccctgat tttgctaacg ctgttgcgat ggctgttgct
      241 atgatctgta tcgtatggat tgccgttttg tctatgactg attgaagcgt tgggcggagg
      301 ctgtgtgaaa caatattggg cgttgggtcg tctgattcca atcgggcttg gggaatgcga
      361 aacggtgtgc gcttatactg cggacgattt gttgcgtggt tttacgcccg aaacggatgg
      421 aaaggtgtgg gaagcggtct gtcggagtag aatacgcgtt ttgcgtttga atgcagcaat
      481 aagaaaagag agaaacttat gccgtctgaa catcaacaca catcatcatt gcttgatttc
      541 gaccgtaccc atctgctcca tccctatact tccatgaccg atccgcttcc cgtttatcct
      601 gtcaaacgtg cagaaggggt gtttatcgaa ttggcggacg gcacgcggct gattgacggg
      661 atgtcgtctt ggtggtgtgc gatacacggt tacaatcatc ccgttttgaa tcaggcggtt
      721 gagacgcaga tgaaacagat gtcgcatgtg atgttcggcg gtttgacgca tgagccggcg
      781 gtggagctgg gcaaattgtt ggtcgggatt ttgccgcagg ggctggatcg tattttttat
      841 gcggattcgg gttcggtttc ggtggaagtc gcgttgaaga tggcggtgca ataccagcag
      901 gcgcggggtt tgcgtgcaaa acaaaatatg gcgacggtgc ggcgcgggta tcacggcgat
      961 acttggaacg cgatgtccgt ctgcgatccg gaaacgggga tgcaccatat tttcggcagc
     1021 gcgttgccgc agcgttattt tgtcgataac ctgacaagcc gtttcgacga tgaatgggac
     1081 gaggcggatt tgcagcctgt ccgcgccttg tttgaagcgc atcatacgga cattgccgcc
     1141 tttattttgg aaccggtcgt gcagggcgcg ggcggtatgt atttttatca tccgcagtat
     1201 cttcgcggat tgcgcgattt gtgcgacgag tttgatatcg ttttgatttt tgatgaaatc
     1261 gccaccggat tcgggcgcac gggcaagatg tttgcctgcg aacacgcgga ggtcgtgccg
     1321 gatattatgt gcatcggcaa gggtttgagc ggcggctata tgacgctggc ggcagcaatc
     1381 acttcgcaaa aagttaccga aacgatttcg cgcggagaag cgggcgtgtt catgcacggc
     1441 ccgacattta tggcgaaccc gctggcgtgt gccgttgcct gcgcttcggt caaactgctt
     1501 ttgtcccaag attggcaggc aaatatccgc cgcatcgaaa gcatcttaaa atgccgtctg
     1561 aaaaccgcgt gggacattcg tggtgtgaaa gacgtgcgcg ttttgggcgc catcggcgtg
     1621 atcgagttgg aaaaaggcgt ggatatggcg cgttttcaag cggattgcgt ggcgcagggc
     1681 atttgggtgc gcccgttcgg caggttggtg tatctgatgc cgccttacat catttcagac
     1741 ggcgttttga ccaaacttgc cgacaaaacc gtgcaaatct tgaaggaaca cagcaaatga
     1801 aaggcgttta cttcgtcagc ggcatagaca cggacatcgg caaaaccgtc gccaccggcg
     1861 tgttggcaaa acaattgttg cagcagggca aaagcgtgat tacgcaaaag cccgtgcaaa
     1921 ccggttgcca aaacattgcc gacgacatcg ccgtccatcg taaaatcatg ggtaaaccca
     1981 tgcaggaagc cgacaaacaa gggctgacca tgcctgaaat cttcagttat ccagcttcgc
     2041 ctcatctcgc tgcccgtctt gacggcaggg ctttggactt ggacaaaatc cgcaccgcca
     2101 cacaagaatt ggcggcgcag tacgaagtcg ttttggtcga aggcgcgggc ggattgatgg
     2161 ttccgctgac ggaaaacctg ttaaccattg attatatccg tcagcaaggc tatcccgtca
     2221 tcctcgttac cagcggacgg cttggcagta tcaaccacac tttactcagt ttcgcagcgc
     2281 tcaaacaata cggcattcgc ttgtacagcc tcgtgttcaa ccacatccac gacagccgcg
     2341 acgcacacat cgcccaagac agcctgagct acttgaaatg ccgtctgaaa gccgattttt
     2401 ccgaagcgga atggatggag ttggcaaaaa cagacgcggt ataaagattg ggaaaaatat
     2461 ggaacaccta tatgatgaat atggaaatac aacgccaaat agaagaacaa caaataaaaa
     2521 agatgaaact atttcaagta aagtaactgt tgcagcgata ccagtagccg ctccatttgc
     2581 tattgctgat atagtcgatc aggattttat ccaagcgatt atgaaaatta ctggtcattg
     2641 attatctata tattggtggt tgttatgaaa tgctatatta aagttgtcat tattgttttt
     2701 ataatggtaa tgacaacttc ttgtaatcct tgcttttttg caacttcttc ttgtgattat
     2761 tcttttagaa aaaactatcc tataaatagc gacgatgaat attatgttaa gaataatacg
     2821 agcaaagaaa caagaattgc ggatttacgc tattgtattt cttatgcatg tccagaagtt
     2881 tatcaaaaat atcaactacc agatgaaaat ggagattata gggagattat tcgtagaaaa
     2941 tataaagatg attataaaaa gggctataag aatgcagatg atgtcatgac atgctacgga
     3001 aagattggag aatgcatgaa aaataataaa ggatattcaa caaaatggta atgttccgct
     3061 tattatcaac agcccttgta ttgttgataa tgcagggctg ttattacaca actattgata
     3121 atgaaaaatg ctttgttgcc ccacaaagcg ttcattgtcc tgtaagttct gatattgaaa
     3181 atgttattag aaaaaatcct aataaaagca taaatcaagt taggagtgat attaaatatt
     3241 gtgctaatat gtatttttcc aatatacggg aattaaatat tccgcaagat tttgataatt
     3301 acattttcat gcttaaagaa aaaggtattc caagaaatga taaggaattg gcttataatg
     3361 cagttagaaa tatgcacatt atttgtttga gttctttggg tggttatgat tattatcaaa
     3421 aaaccgcaga atttcagaaa atagattata aaaaagaaat ggagaaattg ttaaatgaaa
     3481 accattaaat ttgctattgc cgatatagtt gatagcgact ttatccaagt gattatgaaa
     3541 ttaagcgggc attgatttat gattacgcgt tgttttaaag ttgtcattat tgtttttata
     3601 atggtaatga caacttcttg taatccttgc ttttttgcaa cttcgtcttg taattatcct
     3661 tttgatgata tgacgataag agcaaacaac gatattgaat attttgttaa aaataatacg
     3721 actaacgaac aacgaattga tgatttcaga agttgccttt ccgttgcttg tcctgaaata
     3781 tataaattat atcaattacc tgatgaaaag ggtaattatc aatatattat aaaaaaatat
     3841 tatgataata aatacaatga taatgcagtc aaatcgacaa attgtattgg ggataatgca
     3901 agatgtatgc ttaataataa aggatattca acaaaatggt aataatgggt tacggctttg
     3961 aggaatggct gcccgacctg cccgcccctg tttcagacgg catcggcctg ccgatgagcc
     4021 ggttattgaa agcccggtcg ctgaccgccg cattgcgcgc cttgccgcat acattttcgg
     4081 tggaactgct gaaactgggc gaattggaga cggaatgcgg agggaggctg gtgcgcgaag
     4141 ttttgttgaa gctggacggt atccctgttg tcgcggcaag gagcgagtgc cgtatcggtt
     4201 cggcgttttg gcaaaacatt ttggactgcg gcacgcgtcc tttgggcgag cgtctgtttc
     4261 aagccgattt ggaaggggcg cgttcggcgt ttgagtttgc cgtttccggc gaaggatgcg
     4321 gacggtactt tgccgcgcgg cgttctcggt tttcccgtca cggcgaggaa atgctgctga
     4381 ccgagtattt tctgcccgaa ctgaaacgct ttatcggata aaataccgtt ttttcaagct
     4441 gtgcggcaat atgaatccta aatccccttt atttttacga ctgtccgatc gtttggatgt
     4501 gtacctgcgc ctgatgcggg cggacaagcc cattgggacg ctgcttttgc tgtggccgac
     4561 ctactgggca ttgtggctgg cttcagacgg cattcccgat ttggcggtat tggcggcgtt
     4621 tacaatcggc acgtttttaa tgcggagtgc cggttgcgtc atcaacgatt ttgccgaccg
     4681 cgattttgac ggtgctgtcg agcgcaccaa aaaccgtccg ttcgcacagg gtcgggtcgg
     4741 aaaaaaagaa gcgctgctgc tgacggcatt tttgtgcctg cttgccgcat tgtgcctgat
     4801 tccgctgaat catctgacat ggctgatgag cctgcccgcg ctgtttcttg cgctgactta
     4861 cccgtttacc aaacgctttt ttccgattcc ccaactctat ctcgggcttg ccttttcctt
     4921 cggtatcccg atggcgtttg ccgccgttgc cggaaacgtg ccgcctcaag cgtggatact
     4981 ctttgccgcc aatgtgttat ggactctggc gtatgacacg gtttatgcaa tggcggacaa
     5041 agaagacgat ttgaaaatcg gcatcaaaac ctccgccgtc acgttcgggc gttacgacat
     5101 cgccgccgtt atgctgtgtc atggcggctt taccctgctg atggcagtat tgggtgcggt
     5161 tatcggtgcg gcatgggcat attggacggc aatccccatc gtcctgctgc tgcaataccg
     5221 ccaatatgcc gccatcaaaa gccgcgtccg gcaaatctgt tttgaaacgt ttttggcaaa
     5281 caacagaatt ggttgggtgt ggtttaccgc catttttgcc catacgtttt tcgcgaaata
     5341 aggcagggca atgccgtctg aagagccgta aactgctttg gacggcattt ctatctgtgc
     5401 cgaaaagcgt taaaatatgt ttttaaaacg ctgtgttatg tcagcccgta ccgtatgcgg
     5461 gattgagatt tgccctagca gtcggtacaa tctttctgtt ttgcgatgtc tgaaaagaga
     5521 agcttatgag ccttatcggc gaaattttgc ctttgtccca tattgttttg gatatggagg
     5581 tgggcagtaa aaaaaggctg tttgaggaag cgggcctgct tttggaacgc gaatcctcat
     5641 tgtcccatgc tgatgttttc gaatgtcttt ttgcccgtga aaaactcggt tcgaccggtt
     5701 tggggcaggg cgttgccatc ccgcacggcc gtcatgccgg cgtgaagcag gcgacgggcg
     5761 cgttcatccg cacgcgcgaa cccgtcggat ttgacgcacc ggacggcaag ccggtttccc
     5821 tgatttttat cttgctggtg ccggaaaacg caactggcga acatttggaa gtcttatcca
     5881 aactggccgg caagttttcc caaaaaagca tccgcgaatc gctgatgacg gtttcttctg
     5941 cggaagaagc ccgcgccatt ttgactgaag aataatatgc ccagtatttc cgtccgccga
     6001 ctgtttgacg acaaccaata caagttacaa cttgcctggg ccgccggcaa ttcgggtgcg
     6061 gacaaccgta tcggcgtaga ggcggacaag cccgtcctcg ctttggtcgg acacctgaat
     6121 ttcatccacc ccaaccaaat ccaagtggtc ggtttggcgg aatcggaata tctgaaccgt
     6181 ctcgaatcgg gggaaacggg ttatcagttc ggcgacctgt tcgacatttc tatgtctttg
     6241 gttattgtgg caaacggctt gccggtttcc ccggggctgc gcgactattg ccataaaaac
     6301 gatattccac tgctgacttc caaactcgaa agcccctatc tgatggacgt gttgcggatt
     6361 tacctgcaac gcaccctggc ggcatcgtcc gtcaaacacg gcgtatttct cgatgtgttt
     6421 gaaatcggcg tgctgattac cggacattcc ggcttgggca agagcgaatt ggcgttggaa
     6481 ctgatttcgc gcggccacag cctgattgcc gacgatgcgg tcgagctgtt ccgcatcggt
     6541 ccggaaacat tggaagggcg ttgttcgcct atgctgcgtg attttttgga agtgcgcggc
     6601 ttggggatac tcaatatccg ccatattttc ggcgaaactt ccatccgccc caaaaaaatc
     6661 ctgcaactca ttatcaattt ggtcgaggca gacgacgagt atatgaagca gctcgaccgg
     6721 ttgagtatcc gcaccgaaac cgaatccatc ctcaacgtca acgtccgttc ggttacgctg
     6781 cccgtcgccg tcggacgcaa cctcgccgtt ttggttgagg cggcggtacg caattacatt
     6841 ttgcagttgc gcggtaagga cagtacgcgc gaatttttgg aacgccatca gacgcaactt
     6901 aaagaaaacg aacagaatca tgaaaatcgt cctgattagc gggttgtccg gttcgggcaa
     6961 atccgtcgca ctgcgccaaa tggaagattc gggttatttc tgcgtggaca acctgccctt
     7021 ggaaatgttg cccgcgctgg tgtcgtatca tatcgagcgt gcggacgaaa ccgaattggc
     7081 ggtcagcgtc gatgtacgtt ccggcattga catcggacag gcgcgggaac agattgcctc
     7141 tctgcgcgga ctggggcaca gggttgaagt tttgtttgtc gaggcggaag aaagcgtgtt
     7201 ggtccgccgg ttttccgaaa ccaggcgagg acatcctctg agcaatcagg atatgaccct
     7261 gttggaaagc ttaaagaaag aacgggaatg gctgttcccg cttaaagaaa tcgcctattg
     7321 tatcgacact tccaagatga atgcccaaca gctccgccat gcagtccggc agtggctgaa
     7381 ggtcgaacgt accgggctgc tggtgatttt ggagtccttc gggttcaaat acggtgtgcc
     7441 gaacaacgcg gattttatgt tcgatatgcg cagtctgccc aacccgtatt acgatcccga
     7501 gttgaggcct tacaccggta tggacaagcc cgtttgggat tatttggacg gacagccgct
     7561 tgtgcaggaa atggttgacg acatcgaaag gtttgttacg cattggttac cgcgtttgga
     7621 ggatgaaagc aggagctacg ttaccgtcgc catcggttgc acgggaggac agcaccgttc
     7681 ggtctatatt gtcgaaaaac tcgcccgaag gttgaaaggg cgttatgaat tgctgatacg
     7741 gcacagacag gcgcaaaacc tgccgagccg ctaatcccgt tgaacgatga tgccgtctga
     7801 aacccgattt tctgtttcag acggcatttc aaatattgag aagaaaggaa agacatggca
     7861 atccaatggt ttcccggcca tatgaacaaa gcgaaaaaag ccatcgccga gcgcgccaaa
     7921 agcgtagaca tggtgattga aatgctggat gcgcgaatgc ccgcctccag cgaaaacccc
     7981 ctgcttgccc agctttccaa aggcaaaccc aaacttaaaa tcctaaacaa acaagatctt
     8041 gccgaccccg aacgcacaaa ggtctggctt gaccactaca acagccgccc cgacacgtgc
     8101 gccattgccc tcgattcctc cgaaaccggc gcacacggca aaatcaccca agcctgccgc
     8161 gcgatgattc cccatcggca aggcatagac aaacccttgc gcgtcttgat ttgcggcatt
     8221 cccaacgtcg gcaaatccac cctcatcaac ggcatgatag gcaaaaaatc cgccaaaacc
     8281 ggcaacgaac ccggcatcac caaagccgaa caacgccttt tccttgccga cgacttctgg
     8341 ctgtacgaca cccccggaat gctgtggccg aaaatcattg tcgaagaaag cggctacaac
     8401 ctcgccgccg gcggcgcagt cggacgcaac gcattggacg aagaagaagt cgccctcgaa
     8461 cttttagact acctccgccg ccactacctc cctatgttgc aagaacgcta ccaagccgac
     8521 aaagacccca gcagccactg ggacgaaaac gtttggctcg aatggatagc caaaaaacgc
     8581 ggcgcagtcc tcagcggcgg acggatcaac taccaaaaag ccgccgaaaa catcctcacc
     8641 gacttccgtg aaggcaaaat cggcagaatc accctcgaaa cgccgaacca atgggaaact
     8701 tggctcaaaa aagcccgtca gaaagaagcc gaactcaaag ccatacgcga agccagaaaa
     8761 gcagagcgta aagggaagaa gccttcggaa gcataaagaa tgccgtctga aaaatatttt
     8821 tcaggcagct tctctctact ccaaccgatt tcagacggca tatccaaacc catgccgttt
     8881 cagcacggat acccgtatga ccgacaaaat ttctcccgac gcgctgattg aagccgcact
     8941 gctgacccaa accgaaccgc tgaccgaaaa atctatgcgc gaattgtgtg tgccgccact
     9001 gtcgcaggac aaactgattg atgtgttggc gcagttgaaa acgcgttggc aggatagggc
     9061 gttgcaactg gtgcatacgc aagagggctg gcgttttcag attgttcaga cggcattcga
     9121 gcggctgggc agcctgcaag aacagcgtgc gccgcgctac tcccgcgccg tgatggaaac
     9181 actggcgatt atcgcctacc agcagcccgt aacgcgcggc gacatcgaag gcatacgcgg
     9241 tgtggcggtg tcgcagaacg tgatgcagac cttgcaggat cgggggtgga ttgaagtcat
     9301 cggacatcgg gacacattgg gaaaacccgc attgtgggcg acaacggcaa cttttctcag
     9361 cgatttgcgc ttggacggtc tggaagaact tccgccgctg accgaactgg gcgaactggt
     9421 tttacccgat ttaatggaaa tgccgcctac ggatgaagaa gagccggaag ccgtaccgtc
     9481 cgataccctg cccaactgaa attccaaatg ccgtctgaaa cgcacattgc ttcagacggc
     9541 atttcaacaa ataagcagat aaaaacaagc actaagcaaa attaaggaaa aacttatttt
     9601 taatttaaaa aatcttagtt ataattcgta tatctaaagt tgatattgct tttgtcggta
     9661 gaattgctaa ggaatcctca cgatgcttct aacactttct ttgcgtgatt ttgtcattgt
     9721 cgaaaatctg aatctggatt ttcaaagcgg ctttaccgta ttgaccggag aaactggcgc
     9781 gggcaagtcc attactttgg atgcgattgg tctgctgttg ggcgataaag ccgattacag
     9841 ccaagtccgc agcggcgcaa aagaagcgca gttgtcggcg ttgtttgata tttcccattt
     9901 acctgtttta aaagcagaat tgtatgaaca ggggctttta aacgacggag aagaagaact
     9961 cagtatccgc cgcattatcg atgccaaagg caaaagccgc agctttatca acaatcaggc
    10021 cgctaccttg gcgcaactca aagccgtcgg cagccagctt atcgacatcc atgggcaaaa
    10081 cgcccatcat tcgcttaatc aggaagccgc ccagcgcgaa ttgttggacg catttgcggg
    10141 cagcagggcg caggcggaaa ccgtcaggca gctttatcaa aactgggcaa actcaaaaaa
    10201 agcattgcag gaagcgcagg aacaggcaga aaacattgca ctcgaacaag agcgtctgga
    10261 atggcagttt aacgaattga atcagttgga cattaaacaa ggcgaatggg aagccctcag
    10321 ccaaagccac gacagccttg cccattctgc cgagctgttg caggctgccg aagaagtcgg
    10381 aagcaagatt gacggcgaca acggcatcca acgccatatc tatcaatgtc aaaaactatt
    10441 ggccaatctg caaaacatcg agccgcgctt tgccgagagc ctgaatatgt tggcaagcat
    10501 cgaagccgaa ttgggcgaaa tcagtgccaa tatgcgcgat gtggcaggtc gcagcgacat
    10561 caatcccaac gaacttgccg cacaagagca gcgtatgggc gagctgatgg ggatggcgcg
    10621 gaaataccgg atcgagcctg aagagttgcc tgccaagttg gcagaaatcg aagaacgcct
    10681 gcaaagcctg caagctgccg ccgatttgga cgcgctcgag cataatgttg cccacaattt
    10741 tgccgaatat caggaagctg cccacatcct ttctgccatg cgccatcagg cggcagagcg
    10801 tttgagcggc gaaacgaccg agcatatgca acaccttgcc atgaaaggtg cacgtttcga
    10861 catcgtcctg ttgccctcgt cgccgacggc acacggtttg gagcaggttc aatttcaagt
    10921 tgccgccaac aaaggcaatc cgccccgtcc gctgaataaa gttgcctccg gcggcgaatt
    10981 ggcgcgtatc agccttgcct tacaggttgt tgccagccaa tatacccaag ttcccaccct
    11041 gatttttgat gaggtcgata ccggtattgg agggggagtg gctgaaatgg tcggcaaggc
    11101 attacgtgcg ttgggcagaa aacatcaggt gcttgccgtt acccatcttc cgcaagtcgc
    11161 atcctgcggc gaaaaccact ggcgagtgcg caagcacagc gagggagagc aaaccgtcag
    11221 cgaaatcagt atattggatg aaatccaacg ggtcgaagag gttgcccgta tgttgggcgg
    11281 agaagtcatt accgatacga cgcggcaaca tgcggcagaa ttgctgcaac ttgcgtcgaa
    11341 aaatagttta ttttaaaatc aatcagttaa aaaataacta aaaataaaag tctaaaacaa
    11401 tagacagaac tcagataaat ccgtattatt acgctttctt aatcacttga acaagtgatt
    11461 gtgctgcacc cgtagctcag ttggatagag tatctggcta cg
//
LOCUS       DAWHXZ010000045        11252 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000045 DAWHXZ010000000
VERSION     DAWHXZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11252
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.045"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..255)
                     /locus_tag="WIE35_000777"
     CDS             complement(<1..255)
                     /locus_tag="WIE35_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ4153720.1"
                     /translation="MVWKTLGEVFDLKNGYTPSKSNKEYWENGSIPWFRMEDIRENGR
                     ILDNSLKHISKSAVKGGKLFPAKSIMMSTTATIGEHALIKV"
     gene            complement(405..878)
                     /locus_tag="WIE35_000778"
                     /pseudo
     CDS             complement(405..878)
                     /locus_tag="WIE35_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(1044..2054)
                     /locus_tag="WIE35_000779"
     CDS             complement(1044..2054)
                     /locus_tag="WIE35_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ4153721.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(2051..3595)
                     /locus_tag="WIE35_000780"
     CDS             complement(2051..3595)
                     /locus_tag="WIE35_000780"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ4153722.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(3668..4672)
                     /gene="rfaD"
                     /locus_tag="WIE35_000781"
     CDS             complement(3668..4672)
                     /gene="rfaD"
                     /locus_tag="WIE35_000781"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ4153723.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
                     "
     gene            complement(4801..5832)
                     /locus_tag="WIE35_000782"
     CDS             complement(4801..5832)
                     /locus_tag="WIE35_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ4153724.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPFIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFCSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKN"
     gene            complement(5834..7069)
                     /gene="dcm"
                     /locus_tag="WIE35_000783"
     CDS             complement(5834..7069)
                     /gene="dcm"
                     /locus_tag="WIE35_000783"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ4153725.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(7106..8077)
                     /gene="hldA"
                     /locus_tag="WIE35_000784"
     CDS             complement(7106..8077)
                     /gene="hldA"
                     /locus_tag="WIE35_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ4153726.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(8132..8872)
                     /gene="pyrF"
                     /locus_tag="WIE35_000785"
     CDS             complement(8132..8872)
                     /gene="pyrF"
                     /locus_tag="WIE35_000785"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ4153727.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAASTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(9398..10045)
                     /gene="adk"
                     /locus_tag="WIE35_000786"
     CDS             complement(9398..10045)
                     /gene="adk"
                     /locus_tag="WIE35_000786"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ4153728.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            10260..11099
                     /gene="htpX"
                     /locus_tag="WIE35_000787"
     CDS             10260..11099
                     /gene="htpX"
                     /locus_tag="WIE35_000787"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ4153729.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FSGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
BASE COUNT         2652 a         2627 c         2670 g         3303 t
ORIGIN      
        1 aacttttatt aacgcatgct ctcctatggt tgccgttgtc gacatcatta ttgatttagc
       61 aggaaacagc ttgccgccct taactgcgct ttttgatata tgcttcaatg aattatctaa
      121 aatacggcca ttttccctaa tatcttccat tctaaaccaa gggattgagc cattttccca
      181 atattcttta tttgatttgg aaggggtata gccattcttc aagtcaaaca cctcccccaa
      241 cgtcttccaa accacatctt tcagacggcc tttatagcca tcagctatcc ccccccgatt
      301 tgattgtcaa aatctaaaag aaagtcgcgg taataccggt attggcgttt gcgcagggct
      361 aattcagctt ccagctcggt gaatttgtca agtattttta caattttttg ttgggtttcc
      421 aggggtggga tagggattaa taattttttc aagttactgg catttagagc aggaatacct
      481 gtaaatgtac ataaggcatg aatctcttgt tcaaattcaa gtaatatatg atagagaaaa
      541 cgaacatcca attctgttct attcttaata gtaactgcat aatttaagtt gccacgaaaa
      601 tatcttcctt cttgccacgt tattgagccg actcccgctc ctcttgttgt gatgccgata
      661 gggtcatttt cagtgttcca ttcatcaata tagccaagcg gctctttgcc actgttaata
      721 actggataac taccaatatt atttgagatt ttctgcttac ttacagcttg tcctgttttt
      781 atgatagcta tcccattttc tccacccaac ggcttccact ccaccggtgc cgtctgaatc
      841 atctcaatca attttttcgc tttgctttgc atatccatgg ttgttttccg tttctttccc
      901 gctgtggcgg gatgtggggc ggttgtgggg tgcttcggct cactctgtcc gtttgccgtt
      961 atttctgcgg gctgaagtcc gtcctgcttt caggctgcct gcaatggtgt gcaggctgct
     1021 ttttcaggta gcctgaatgg tagttatttt gtgcggtctt ttttctgttt gatctgttgc
     1081 acggctgttt tttccaacgc tttaacgctt tccagatagg cttgctccac cggcgacagg
     1141 atgcgcgctt cataggcgcg gtattcgcgt tcggctttct gctctgcctg tttgcggctg
     1201 atgctgcctg caccctgtaa tgtgccttgt ccgtacagcc cggaaaattt gtccaattcg
     1261 gctatccagt cgcgcatata catggggctt tgctcctgcg ctttgatttc cgctaggtcg
     1321 aagaaggcgg aaaccagacg gttcaggcgg aacagttcgt cttcggtcag atagtttttg
     1381 gcgattttgg cttcattcag cgtggggatt gcgccttgaa aggtggtcag ccccataaag
     1441 tctttgctgc tgtcggcacg gctgtatatc agctcagctg cggtttgccg gctggcggca
     1501 tagtgcagtt tgttttgaac ggcggcaaaa aaggtttggc tttcgctgct tttggggttg
     1561 tagtcttggc tggtggcata taaatcaagc acttgccggt atagggcttt ttcgctgctg
     1621 cggatgtcgc gaatgcggtt gagcagttct ttccaatagt cgccgccgcc tgtgcctttc
     1681 aggcgttcgt cgtctatggc aaagcctttg gtcagatatt cgtccagccg ttcggttgcc
     1741 cattggcgga attggatgcc ccgcgcggaa cggacgcggt agccgacggc aataatcatg
     1801 ggcagggaat aatgggcgat tttgcggttt accttgcgcc cgttttcatt ttgaactgtc
     1861 aactggaagt tgacagttgc cttctcttcc aattcttgct ctgcaagaat ggttttaatg
     1921 tgtttgctga tattttgttt ggtggtttgg tacagttctg ccatgtccgc ctgcgtcagc
     1981 caaagctgtc cgccaaattc ctgcaaggca aattgggcag tgccgtcgtt ggcggtgtat
     2041 aggatgatgc tcatgcttca atctctgcaa tcacttcgtc aatttcacgc cgcagccgtt
     2101 cgattttggc aacggtttcg ctgatttcgg cgttaagctg tttgatgtcg ataatttcgc
     2161 gggtgtcttc gggttcaaca tagctgctga cggcgaggtt gtagccgttg tctttgacgg
     2221 tttgctgggc ggcgttttgg gcgatatgcg gcacatcggc tttatcggcg aagagtttga
     2281 cgatttcggc aatgtgttct tcggttaaga cgttgttgtt ggtttctttt ttaaagaagc
     2341 cgcctgcgtc gatgaattgg atgtcggtat tgtctttgtg tttggacaaa accagtatat
     2401 tgacggcgat gccggtgccg taaaagagat tgggcgcaag ggcgatgacg gtttccacgt
     2461 agttgccctc caccagatat tggcggattt tctgctctgc gccgccgcga tagaaaatgc
     2521 cggggaatga gacgatggcg gcgcggcctc tgccggaaag gtagttcagt gcgtgcagaa
     2581 tgaaggcaaa atcggctttg gatttcgggg cgagtacgcc tgcaggggca aagcggtcgt
     2641 cgttgattaa ggtggggtcg ccgctgccta tccagttgat ggaatagggc ggattggaaa
     2701 cgacggcatc aaagggtttg ctgtctttga gtttgggatt ggtcagtgtg tcgcccaatt
     2761 cgatgtggaa tttgttgtaa ttgacgttgt gcagaaacat attcatgcgg gcgaggttgt
     2821 aggtggtgtg gttgatttcc tgcccgaaga agccttcttc gatgatgtgc tcgtcaaact
     2881 gttttttcgc ctgcaagagc aggctgcccg agccgcaagc tgggtcgtag attttgttta
     2941 ctttctcctg cccgtgcacc gccagccgcg caatcagctt ggatacgctt tgcggggtga
     3001 aaaattcgcc gccggatttg cctgcgttgg cagcgtagtt ggaaatcagg tattcgtagg
     3061 catcgccgaa aaggtcgatg tggtggtctt caaaactgcc gaaatcgagt tccgccacgc
     3121 cttttaggac ggcggcaagg cgtttgttct tgtcggcaac ggtgctgccg agccggctgc
     3181 tggtggtgtc gaagtcgtca aacaggcctt taatgtcttg ttcggacgga tagccggagg
     3241 cggagctttc aatcgcggta aaaatttctt tcagcttggt gttgagctct tcgttttgat
     3301 gggcttcggc ggcaatattg caaaaaagct ggccggggta gatgaaatag cctttgactt
     3361 tgacggcatc gtctttgatt tcgggcgtga tgatgctgtc cggcatagcg gcgtaatcaa
     3421 tactgctgtc gcctgcctgc atatagtcgg taaagttttc gctgataaag cggtagaaaa
     3481 gtgtgccgag aacgtattgt ttaaagtccc agccgtccac cgcgccgcgt acttcgtcgg
     3541 caattttcca aatttggcgg tgcagttggg cgcgttgttg catttcggtc atcatcgaaa
     3601 tccatataaa agttaaacaa atcgaaatcg cctgatattt tcagacgatt ttttacgggc
     3661 attcaaatta agccaaattt tccagcatcc atttgacgta gcgctcgacg cctgatttga
     3721 catcgaaaaa ttcttccgta taacctgcct cacgcaattt ggtgatgtcg gcttgagtga
     3781 agctttggta tttgcctttg agcgcatcgg gaaatgggat gtagcggata agttcttctt
     3841 ctaccagctc tttcaagctc atttcgggtt tgccttcggc ggcgcggcag gcgttgacgg
     3901 tggcggcggc gagttcgttg aactgttggc tgcggccggt gccgaggttg tagatgccgg
     3961 aaagttcggg atggtcgaag aagtagaggt tgactttggc aacgtcttcg acgctgacaa
     4021 agtcgcgggt ttgttcgccg ttgccgtagc cgtcgttgct gccgaacagg ttgacgtaac
     4081 cgtgttcgcg gtattggtgg aaatggtgga aggcgacgga tgccatacgg cctttgtgtt
     4141 gttcgtgttg tccgtacacg ttgaagtagc ggaagccgac gacttgggcg gtcaagcctt
     4201 ctttcatgcg gcggcgcaat acttggtcaa acaggaattt ggaatagccg tacacattga
     4261 gcggtttttc gagttcgcgc tcttcacgga agatttcgcc tttaccatac acggcggcac
     4321 tggaggcata aaggaaggga atgcgttcgt cctgacacca gtccaacaaa tccagcgtgt
     4381 actggtagtt gttttccatc atatacaaac cgtcgtgatt catggtgtcg gaacacgcgc
     4441 cttgatggaa aacggcttcg atgttttgat aaggtaaaat gtgttccctc acttggcgga
     4501 tgaattcatg tttgtcgaga taatgggcga tttcgcactc ggcaaggttt ttgaattttt
     4561 cgcctttgct caaattatcg acggcaacaa tgtcagtaat accgcgttga ttaagtgctt
     4621 tgacgatgtt gctgccgata aagccggccg cgcctgttac gatgatggtc atattcggtt
     4681 tcctttgttt tgagtaaaat taataaactt agttacaaga aatcatcgat attgtcctga
     4741 aacacaaatt tgcgatgata ttccaaatat ttttctcttc cttcgggata tattctcatc
     4801 ttaatttttt gcatttggat tgatattaag tttttcccat gtgtaacggc ttagtcgagt
     4861 accgcaaatc aaacgaccat catccaagaa agtaatataa ccttgatcga acaaccaatc
     4921 ataagtcggc gttaaagcta acccatttaa ataatctaat gcctctttct catttttctc
     4981 agtaatacaa accatataag gtttaatatg actagccctt aacaaaatct catctgtaat
     5041 taatgtaaat ggacattgcg gcatatgatt tataacatcc ttgcgatatt tctgctgccc
     5101 ttttctactt ttctgtttaa tgatttcttg ctctacgaat tgattaaatt ctgaagccgg
     5161 tatttctaat ttttctcttg ataagagttg cgggtgcacg atagagcaaa attgataatc
     5221 caaaaatatt cggaaataaa ataatggttc tgaatcttct atatctttga caggcaatag
     5281 tttaagaatt gataaataac taattttggg caatacaatc ttacgccaaa ttgaccatat
     5341 atcatcctcc gaacggatat aacccctacg gtaaccttta gaattttcca aatggtctga
     5401 tacatcatgg attgaaaaga aaatattttc tgtaggcaga ttattaatac ttaaaacgta
     5461 atcatcataa aaccttgaaa tatcatgatg atatttctgt tcttgtttgt gatattcaac
     5521 ttttgcatca accaaataac gctgtaaatt tttcttatta aaaaaacaat ttttctgaat
     5581 gaaaggatat tctaccctcc ttccattttt tgtctcagta atcccccaat tttcatactc
     5641 aaaaaatgta cttagttttc gaccactatc atcaatataa ctacctacat atttgcgtgc
     5701 ttccccatta ccaccaaaaa cagataactt gttattcctg tgaataaaac tatcttccgc
     5761 acgtaaattt tgaatactat cgacaataaa atactctatg ttttcaatag taattctttg
     5821 aaaatccggc attttactct ccataaagcg taatatccat ttgttttaat atagcgatta
     5881 atacatcaac aacaatactg tttcccgctt ggcgatacat ttgagtatca ctaactaaaa
     5941 ttttaaaatc atctctaaaa cccattagcc gcaaacattc cctaggtgtt aatttacgaa
     6001 tacgtccacg attatgagta acataattat caaccccggc acgatgcatt ttatgcatag
     6061 tttgcaataa tgggcgagct acaggcaaat cagtttccgt actggtttta aaattttttg
     6121 ttcctcctgc caaaacataa tttttgattt tttcagaaag ataatatttt tcctcaacgt
     6181 tatttacatc aaaaataaag tcatcaaatt cagattcgcg ggctgcctga aaaataaaat
     6241 caccatgcca attaaattgt tgatttgctt tttggcataa ggcaatttct ccattaatct
     6301 gtgtataacg tttttgtcta tttttagaac tggtaacaaa tttcacaccc ttttcacgta
     6361 aaaaatattt gctatcagta tagtcctcca aaaaatcttg catagtatgt tctaattcaa
     6421 tcttttcagg aaactgaaaa ccatttatag gaggggtatg aaagccaaca acaaaaatac
     6481 gctcacgatg ttgaggaatc ccataatcct tactattcat tatttggaaa tataagtcat
     6541 aaccaagtga ataaaaaaca ctttttacaa ctttccaagt ttttccatta tcatgattga
     6601 gcaagccctt tacattttca taaataaata tctttggttg gacttcatcc acaacgcgag
     6661 caaattcata gaataatgtt cctcgtgtat cttctaatcc tgcacgtttg ccaaccatgg
     6721 aaaatgcttg gcaaggactg cctccaacta aaatatcaac ttgatttcta aactttctcg
     6781 catcaaattg agtaatgtcg ttatgccaaa aatcttcatt taatttatag tttccaagat
     6841 aacttttttt aacgtatgga tcaatatctc ctgcaaaaac aatggtatgg tttaaattta
     6901 atcggtggaa tgcctgttca accgcaccaa tcccgctgaa tacggttgct aatctaatat
     6961 gtgaatcagg tttaagaaaa gttttagatt tccaaccttg ttgactggga aagagcaaag
     7021 ttttttgtaa tcgagtatcg tgtgtctgtg ccattgtcga aatagtcata cttatatcgt
     7081 tctgtttatc ttatcaatat gaaaactaca ttgttgattg ccctgacaat gccttgatca
     7141 attcggcaaa cgagcaaacc gccgtaccga gtttcgccac gacaaccccg gccgcagtat
     7201 tggcaaggta catggcttcg ggcatggtgc agcctgccgc caaacccaag cccattccgg
     7261 caatgacggt gtcgcccgca ccggatacgt cgtaaacttc ttgggcgcgg gtgggctggt
     7321 aaatcggttc gccttcgctg aacaaggtca tgccttcttc gcttcgggtc agtaaaacgg
     7381 cggtcaggtc gaggtggcgg cgcaggtttt gcgctttttc ggtcagctcg ctttcgtttt
     7441 tccaactgcc gaccacttct ttcaattcgg cgcggttagg cgtaatcaga gttgcaccga
     7501 catatttttc gtaatcgtcg cctttggggt cgattaagac ggttttgccg gcgtgtttcg
     7561 cccaatcgat catatcggag atatgcgaca ggccgccttt gccgtagtct gaaaaaatga
     7621 ttgcgtcgta ttcgggcaag atttcgcggt atttctgctt gatttgttcc aacacttcgc
     7681 agttgggatg ttcttcaaaa tcaagacgga taagctgctg gttgcgggcg acgacgcgca
     7741 gtttgacggt ggtggcgatt tgtttgtcgc gcatcagata ggaggcgacg ccgtcctgca
     7801 ccatcagcgc atcgagcgcg tcggcggctt cgtcgttgcc ggttacggac aacagccctg
     7861 ccctgccgcc caacgaagcg atgttgcgcg cgacatttgc cgctccgccc gcgcgttggt
     7921 cgattcgtcc gattttcgcc accggcacgg gggcttcggg cgaaatacgg gacacatcgc
     7981 cgaaccaata gcggtcgagc atcacgtcgc cgacaaccag gactttggct tgcgcgaaac
     8041 gggatttgag ggtttcttgt tggaacttgg cggacatatt gcctccgttg tattttttca
     8101 gacggcctca gcctgtaagg ccgtctgaaa atcagtttgc ttcaaggttt gccacgcggt
     8161 tcacttcgcg caatacggct accggatcgg cagcttgggt tacaggacga cccattacca
     8221 aataagtcga agcggcagcc aaggcttcgg ccggtgtcat gatgcggcgc tggtcatcat
     8281 tattgccggc aacgtccaag cggatgccgg gcgtgaccaa gacaaaatct tgtcccaact
     8341 cgcggcgcag cggtgcggct tcttgggcgg aacagaccac gccgtccaag cccgaacttt
     8401 gcgccagttt tgccaagcgg atgacttgtt cttcaggggc ggtgttcaaa ccgatttccg
     8461 ccaaatcgct ttgttccatg ctggtcaaca cggttacgcc gattaagagc ggctttgtac
     8521 cgtatccggc aacggcttct gctgcggctt ccatcatacg gcggccgccc gatgcgtgca
     8581 tatcgaccat ccaaacgccc atatcggcag cgactttgca ggcttgcgcg acggtgtggg
     8641 gaatatcgtg gtatttcaaa tcgaggaaaa gtttgaaacc ttgattgatt aagctttctg
     8701 ccaaattgcg tcccgtcgcg gtaaacagct ctttgccgat tttgatttga cacaatgtcg
     8761 ggtcaaggtt gcggacgaat ccgagcgtgt ctttttcgtt ggaaaaatca agggcgacga
     8821 tgacgggggt gcgttgttgc ggagtttgga agtcggagat taaaggattc atggtgtttt
     8881 cgtttcgggt tttcagacga cctttatcgt ctttttcgca gtatagtgga ttaactttaa
     8941 accggtacgg cgttgtctcg ccttagttca aagagaacgc ttctctaagg tgctgaagca
     9001 caaagtgaat cggttccgta ctgtttgtac tgcctgcggc ttcgtcgcct tgtcctgatt
     9061 tttgttaatc cactataaca acgtaaattc aaaccgtagg tcgggcattg atgtccgacc
     9121 tacgcggact caagcctccg acaaactgtt tcggataaat ttgaaccggt acatcagccg
     9181 tcattcccgc gcaggcggga atccattgat aaaactcagt tcatcagatt taaacagcga
     9241 ttgcccaaat tcagaaatgg attcccgcct gcgcgggaat gacgccaaat gattattttt
     9301 cggttgcaac acgctgttta tcaaaaacag gtcgtctgaa aacctgattt tctgttttca
     9361 gagtgcgaag cctgcccgtg ggtacgacct tttcgattta tttgcccaat gcgcccaata
     9421 cttcggcttt cacggcttct accggctgag tgccgtcaac tttgatgtat ttaggcgcgt
     9481 gttcgccttc cagtttgctg taaaaatcga ccaaaacttc ggtttgctcg tggtaaacgg
     9541 caaggcgttt tttcacggtt tcttctttgt cgtcgtcgcg ctgaatcaaa tcttcgccgg
     9601 ttacgtcgtc tttgccttca actttgggcg ggttgtaggt aacgtggtaa gtacggccgg
     9661 aagccaaatg cacgcggcgg ccgctcatgc ggtcgacaat cacgctgtca ggcacatcga
     9721 tttcaacgac tgcatccaaa tccacgcctg cttcaaccat cgcttcggct tgtgccaatg
     9781 tgcgcgggaa accgtcaaac aggaaaccgt ttttgcagtc gtcttgcgcg atgcgttctt
     9841 tgaccatgcc gataatgatg tcgtcgcgca ccaagccgcc ttcgtcaatg atttttttcg
     9901 cttccaaacc caagggcgtg cctgccttaa tcgcggcacg gagcatgtcg ccggtagaga
     9961 tttgcggaat gccgaacgct gcggtgatga attgcgcctg agtgcctttg cccgcgcccg
    10021 gtgcgcctaa aagtaatgct ttcatgaagt gatcctttta aatgtgttta ttggaatcaa
    10081 acagatggtt tacgattcta cacggcttgg cacaatagtt caaaaaccaa ctgcacaaac
    10141 tgccatttgc aagcaaatgt tttgcatacc tttgccgagc ctgatattca caaggcggca
    10201 actttttggg acaatcccca aaatttcaaa acatcacacc ctaccaaaaa ggaacatccg
    10261 tgaaacgcat ttttctgttt ttggctacca atatcgctgt tttggtcgta atcaacattg
    10321 ttttggcggt tctgggcatc aacagccggg gcggcgcggg cagcctgttg gcgtattccg
    10381 ccgtcgtcgg cttcagcggc tcgattattt cgctgctgat gtccaaattc atcgccaaac
    10441 aatcggtcgg tgcggaagtc atcgacacgc cgcgcaccga agaagaagcc tggcttttga
    10501 acactgtcga agcccaagcg cggcaatgga acctgaaaac gcccgaagtc gccatctacc
    10561 actcccccga acccaatgcc tttgccacgg gcgcatcgag aaacagctcc ctgatcgccg
    10621 tcagcaccgg tttgctcgac catatgacgc gtgacgaagt ggaagccgta ttggcgcacg
    10681 aaatggcaca cgtcggcaac ggcgatatgg ttacgctgac gctgattcaa ggcgtggtca
    10741 atacctttgt cgtgttcctg tcgcgcatta ttgccaacct gattgcccga aacaacgacg
    10801 gcagccagtc ccagggaact tatttcctgg tcagcatggt attccaaatc ctgttcggct
    10861 tccttgccag cttaattgtc atgtggttca gccgacaacg cgaataccgc gccgacgcgg
    10921 gcgcggcaaa actggtcggc gcgccgaaaa tgatttccgc cctgcaaagg cttaaaggca
    10981 acccggtcga tttgcccgaa gaaatgaacg caatgggcat cgccggagat acgcgcgact
    11041 ccctgctcag cacccaccct tcgctggaca accgaatcgc ccgcctcaaa tcgctttaaa
    11101 ccgatttgaa acggcaaaaa ccgtacgcgc aagcagtacg gttttttgta tatgctgtct
    11161 gaaaacatca ggtttccata ccgacaccaa ccgtatccat ttccgcaccg gatacgccgc
    11221 attgatatac cctgataaac cgtatccgga ca
//
LOCUS       DAWHXZ010000046        11184 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000046 DAWHXZ010000000
VERSION     DAWHXZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.046"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..3634
                     /gene="purL"
                     /locus_tag="WIE35_000788"
     CDS             <1..3634
                     /gene="purL"
                     /locus_tag="WIE35_000788"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ4153730.1"
                     /translation="ERGMAVWLEGRLNDDEKQQWAALLHDRMTESVLPDFQTASKLFH
                     HLESETFSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMF
                     AQANSEHCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAK
                     IERFYPDAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGK
                     GSRPKAGLTGFTVSNLNIPCLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGR
                     PNLLGYFRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGM
                     LIGLGGGAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHD
                     VGAGGLSNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDL
                     DTFRAICERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKPPKTTRTDKT
                     VTPSKKPFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTP
                     VADCAVTMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNI
                     KLSANWMAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKS
                     VVSPLSLIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNM
                     SGDAPDLDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDID
                     LNLLLAQTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADI
                     INLFYQQQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRD
                     NPACADSEFALIGDNDRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIE
                     MAAAFTRAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFH
                     PALRDQFAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARLSM
                     VHVPKSASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQ
                     TYPLNPNGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLFAG
                     ARKALG"
     gene            3729..4487
                     /gene="gloB"
                     /locus_tag="WIE35_000789"
     CDS             3729..4487
                     /gene="gloB"
                     /locus_tag="WIE35_000789"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ4153731.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            4794..9267
                     /gene="mspA"
                     /locus_tag="WIE35_000790"
                     /gene_synonym="ausI"
                     /pseudo
     CDS             4794..9267
                     /gene="mspA"
                     /locus_tag="WIE35_000790"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242022.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
     gene            9499..10953
                     /gene="mgtE"
                     /locus_tag="WIE35_000791"
     CDS             9499..10953
                     /gene="mgtE"
                     /locus_tag="WIE35_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ4153732.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLGELHPADVAAVLESLPPRERNVVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(11039..>11184)
                     /locus_tag="WIE35_000792"
     CDS             complement(11039..>11184)
                     /locus_tag="WIE35_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153733.1"
                     /translation="CWDFGCREGKEFCKGLSKYVFDETDVNALFGEDVVGVAKGLPRH
                     TVQ"
BASE COUNT         2873 a         3518 c         2748 g         2045 t
ORIGIN      
        1 cgagcgcggc atggcggtgt ggctggaagg tcgtctgaac gacgatgaga aacagcaatg
       61 ggcggcgttg ctgcacgacc gcatgaccga atccgtgctg cccgattttc agacggcatc
      121 caaattattc caccatctcg aatccgaaac cttttccacc gttgatgttt taggcggcgg
      181 taaagaggct ttggtcaaag ccaataccga aatgggtttg gcactctccg ccgacgaaat
      241 cgattatctg gttgaaaact atcaggcttt gcagcgcaat ccgtccgatg ttgaattgat
      301 gatgttcgcg caggcaaaca gcgaacactg ccgccacaaa atcttcaacg ccgatttcat
      361 cctcaacggc gaaaaacaac cgaaatccct gttcggcatg atacgcgaca cgcacaacgc
      421 gcatcccgaa ggtacggtcg tcgcttataa agacaattcg tccgtaatcg aaggcgcgaa
      481 aatcgagcgt ttctatccgg atgcggcgga aaaccaaggc taccgtttcc acgaggaaga
      541 cacgcatatc atcatgaaag tggaaacgca caaccacccg accgccatcg cgccgtttgc
      601 gggcgcggca acgggcgcgg gcggcgaaat ccgcgacgaa ggtgcgacgg gcaaaggttc
      661 gcgtccgaaa gcaggcttga ccggctttac cgtgtccaac ctcaacatcc cctgcctcaa
      721 acagccgtgg gaacaagatt acggcaagcc ggaacatatt tcctcgccgc tggacattat
      781 gattgaaggc cccatcggcg gcgcggcgtt caacaacgaa ttcggccgcc ccaacctctt
      841 gggctacttc cgcacctttg aagaaaaatt tgacggtcag gttcgcggct accacaaacc
      901 gattatgatt gccggcggtc tgggcagcat tcaggcgcag cagacgcata aagacgaaat
      961 ccccgaaggc gcattgctga ttcaactggg cggcccgggc atgcttatcg gcttgggcgg
     1021 cggcgcggct tcttcgatgg ataccggcac gaacgacgcg tctttagact tcaattccgt
     1081 acaacgcggc aaccccgaaa tcgaacgccg cgcgcaggaa gtcatcgacc gctgctggca
     1141 gctcggcggc aaaaacccga ttatctccat ccacgacgtg ggcgcgggcg gtttgtccaa
     1201 cgccttcccc gaactcgtca acgatgccgg acgcggcgcg gtattcaagc tgcgcgaagt
     1261 gccgttggaa gaacacggtt taacgccttt gcaaatctgg tgtaacgaat cgcaagagcg
     1321 ttatgtgttg tcgattttgg aaaaagattt ggacaccttc cgcgccatct gcgaacgcga
     1381 acgctgcccg tttgccgtgg tcggcacggc gaccgacgac ggccatttga aagtgcgcga
     1441 cgatttgttc gccaacaacc ccgtcgattt gccgctgaac gtcttgctcg gcaaaccgcc
     1501 caaaaccacg cgtaccgaca aaacggttac gccgtccaaa aaaccgtttc acgccgacga
     1561 tatcgacatc accgaagccg cctaccgcgt tttgcgcctg cctgccgtag ccgccaaaaa
     1621 cttcctgatt accatcggcg accgcagcgt cggcggcatg acccaccgcg accaaatggt
     1681 cggcaaatac caaacccccg tagccgactg cgccgttacc atgatgggct tcaataccta
     1741 tcgcggcgaa gcaatgtcta tgggcgaaaa accgaccgtt gccctgtttg acgcgcccgc
     1801 ctcgggcaga atgtgcgtcg gcgaagccat caccaacatc gcggcggtca acatcggcga
     1861 catcggcaat atcaaactct ccgccaactg gatggcggcg tgcggcaacg aaggcgaaga
     1921 cgaaaaactc taccgcaccg tcgaagcggt ttccaaagcc tgtcaggcat tggatttgag
     1981 cattcccgtg ggcaaagaca gcctgtcgat gaaaaccgta tggcaggatg gcgaagagaa
     2041 aaaatccgtc gtttcgccgt taagcctgat tatctccgcg ttcgcgccgg ttcaagacgt
     2101 acgcaaaacc gttacgcccg aattgaaaaa cgtcgaggac agcgtattgc tgtttgtcga
     2161 tttgggcttc ggcaaagcgc gtatgggcgg ctctgcattc ggtcaggtat acaacaacat
     2221 gagcggcgac gcgcccgatt tggacgacac aggccgtctg aaagcgtttt acaacgtgat
     2281 tcaacagctt gtcgccgaag acaaactctt ggcataccac gaccgcagcg acggcggctt
     2341 gtttgccgtt ttggtagaaa tggcgtttgc agggcggtgc ggcttggata tagatttaaa
     2401 tttattgctt gcacaaacat ttattaccaa ccataccgct ctgtctcaat cattgcggac
     2461 tgaagaggta aaagcgttgg ctgaatggca agaaaccatt gcccgcacat tatttaatga
     2521 agagttgggt gctgttatcc aagttagaaa acaagatgtt gccgatatta tcaatttatt
     2581 ctatcaacaa cagctgcatc ataatgtctt tgaaatcggt acgttaactg atgagaacac
     2641 gttaatcatc cgcgacgggc aaacgcacct tatttctgac aacctaatca aactgcaaca
     2701 aacctggcaa gaaaccagcc atcaaatcca acgcctgcgc gacaaccctg cctgcgccga
     2761 cagcgagttt gccctgattg gcgacaacga ccgcagcgca ttgtttgcca acctgaaatt
     2821 cgacgtgaac gaagacatcg ccgcgccgtt tatcaacagc ggcgcgaaac ccaaaatcgc
     2881 catcctgcgc gaacagggcg tgaacgggca aatcgaaatg gccgccgcgt tcacccgtgc
     2941 cggattcgat gcctacgacg tgcatatgtc cgacctgatg gcaggccgct tccgccttgc
     3001 cgacttcaaa atgctggcgg cgtgcggcgg cttcagctac ggcgacgtac tcggcgcggg
     3061 cgaaggctgg gcgaaatcca tcctgttcca ccctgctctg cgcgaccagt ttgccgcctt
     3121 cttcgccgac ccggacacgc tgacattggg cgtgtgcaac ggctgccaaa tggtcagcaa
     3181 cctcgccgag attatcccgg gtgcagaggc atggccgaag ttcaaacgta acttgagcga
     3241 acagttcgaa gcgcgcctga gtatggttca cgtccccaaa tccgcgtcgc tgattctgaa
     3301 cgaaatgcaa ggctccagcc tgcccgtcgt cgtcagccac ggcgaaggcc gcgccgactt
     3361 cgcgcttcac ggcggcaaca tttctgctga tttgggcatc gcgctgcaat atgtggacgg
     3421 acaaaaccag gttacccaaa cctatccgct caaccctaac ggctcgccgc aaggcatcgc
     3481 cggtgtgacc aacgccgacg gccgcgtgac cattatgatg ccccaccccg aacgcgtgta
     3541 ccgtgccgcg caaatgagct ggaaaccgga aggctggacg gaactgtccg gctggtaccg
     3601 cctctttgcc ggcgcacgta aagccttggg ctaaccgccc tactcaaacc aatgccgcct
     3661 gaagaatatt tcagacggcg ttccggcata ccatccttta aacggtatcc gtccaccgag
     3721 gaacactcat gaaaatcacc cccgtcaaag ccttgaccga caactacatc tggatgatac
     3781 agcacggcaa ccatgccgtc tgcgtcgacc cttccgaacc ctcgcccgtc ttggaattcc
     3841 tcgtccgcaa ccgcctcatg cttgcccaaa catgggtaac tcacccccat cccgaccacg
     3901 agggcggtgc ggcggcactc tggcgcggct acatggaatc gcccgtttac ggcgaatccg
     3961 acatcgaagc agcaacccac accgtaaccg ccggcaccca attcaccttc ggcgacggac
     4021 aggttaccgt ttgggcaaca cccggccaca cagaccgcca caccagctac cttctcgaaa
     4081 cttcagacgg catacacgtc ttttgcggcg acaccctttt ttccgccggc tgcggacgcg
     4141 tgtttaccgg cacaatcgaa cagctttacg acagcttcca acgcttcaac cgcctgcctg
     4201 aaaacaccct gttctatccg gcgcacgaat acaccgccgc caacctggat tttgccgccc
     4261 atatcgagcc ggacaacgcc gacattcaga cggcactgaa agcggcggcg catacgccta
     4321 ccctgcccgt taccctcgcg cacgaacgcc gtgtcaatcc gtttttgcgc gtcgacctgc
     4381 cgcacgtcag agaccgcgcc gaggcattga gcgggaaaac gttaaacagc agcctcgata
     4441 cctttgtcgc gctgcgtgaa cttaaaaacc aataccggac gaaataaaac aacgggaaaa
     4501 cgcagccatt cctaggattt ttattaaaat cttaaataaa atcatacaat catcgccaat
     4561 agacgaaagg acaccgttgc cttataatca aacaaaaaca aaatatataa tatagtggat
     4621 tgaatttaaa tcaggacaag gcgacgaagc cgcagacaat acagatagta cggcaaggcg
     4681 aggcaacgct gtactggttt ttgttaatcc actatataaa tccagcacaa aacgggatcg
     4741 gtgattcttg tccgcaagaa tcgttgattt tctctattac acggataatc atcatgcgct
     4801 tcacacacac caccccattt tgttccgtat tgtccaccct cggtcttttt gccgtttccc
     4861 ctgcttactc atccattgtc cgcaacgatg tcgattacca atattttcgc gactttgccg
     4921 aaaataaagg cgcgttcgcc gtaggtgcaa gcaatatttc catccaagac aagcaaggca
     4981 aaatattagg cagggttctc aacggcatcc ccatgcccga cttccgcgtc agcaaccgcc
     5041 aaaccgccat cgccaccctg gttcaccccc aatacgtcaa cagtgtcaaa cacaacgtcg
     5101 gctacggttc catacaattc ggcaacgaca cccaaaatcc agaagaacaa gcctatacct
     5161 accgcctcgt atcacgcaac ccgcacccgg actacgacta ccaccttccc cgcctcaaca
     5221 aactggttac cgaaatctca cctgccgcac tcagcagcgt acccttgctt ggaaacggcc
     5281 agccaaaggc caatgcctac ctcgataccg accgcttccc atactttgta cgactcggct
     5341 caggcacgca acaagtccgc aaagcagacg gcacgcgtac acgaaccgcc ccggcatacc
     5401 aatacctgac cggcggcacg ccgctgaaag tattggggtt ccaaaaccac ggcttactcg
     5461 tcggcggcag cctgaccgac caacccctta acacctacgc aatcgccgga gacagcggtt
     5521 cccccctgtt tgccttcgac aagcatgaaa accgctgggt gcttgcgggc gtactcagca
     5581 cctacgccgg cttcgataat ttcttcaaca aatacatcgt cacgcaaccc gaattcatcc
     5641 gttccaccat ccgccaatac gaaacccggc tggatgtcgg gctgaccacc aacgaactca
     5701 tatggcgcga caacggtaat ggcgacagca ccctgcaagg gctcaacgaa cgcatcaccc
     5761 tgcccattgc aaacccttcg cttgccccac aaaacgacag caggcacatg ccgtctgaag
     5821 atgccggcaa aacgctcatc ctatccagca ggttcgacaa caaaacactg atgctggcag
     5881 acaatatcaa ccaaggcgca ggcgcattgc agttcgacag caacttcacc gtcgtcggta
     5941 aaaaccacac atggcaaggt gcaggcgtta tcgtagccga cggcaaacgc gtcttctggc
     6001 aagtcagcaa ccccaaaggc gaccggctct ccaaactggg cgcaggcacg cttatcgcca
     6061 acggacaagg catcaaccag ggcgacatca gcatcgggga aggcactgtc gtactcgccc
     6121 aaaaagctgc ttcagacggc agcaaacaag cattcaacca agtcggcatc accagcggca
     6181 ggggcacggc cgtcctcgcc gacagccagc aaatcaaacc cgaaaacctc tatttcggct
     6241 tcaggggcgg acggctcgac ctcaacggca acaaccttgc ctttacccat atccgccatg
     6301 cggacggcgg cgcgcagatc gtcaaccaca accctgacca agccgcgaca ctgacgctga
     6361 ccggcaaccc cgtcctcagt cccgagcatg tcgagtgggt gcaatggggc aaccgtccgc
     6421 aaggcaacgc ggcggtttac gaatacatca acccgcaccg caaccgtcgg accgactact
     6481 tcatactcaa acccggcggc aacccgcgcg aatttttccc gttaaatatg aaaaactcaa
     6541 caagctggca atttatcggc aacaacaggc aacaggccgc caaacaagtc gcccaagccg
     6601 aaaatgcccg ccccgaccta attaccttcg gcggatactt gggtgagaac gcgcaaacgg
     6661 gcaaagccgc accgagttac agcaaaacca atatcgaatg ggtgaatggt ccaaacaccc
     6721 cacacaagcc ctcgccctat acgaatacgc caacccgcac aggggcaggc gtaccgacga
     6781 ttaccgcctc aaagaaagcg gcaaccccgg cgcctacttc ccatggaatg cctacagcac
     6841 ccaagcctgg gaatttctcg gacatgaccg gcgaagcagc catagaaaaa acccgccata
     6901 tcgcaaatgc cgccgtatac ggcaggcccg aataccgtta caacggcgca ctcaacctgc
     6961 actatcgtcc caaacgcacc gacagcacgc tgttgctcaa cggcggcatg aaccttaacg
     7021 gggaagtctt gattgagggc ggcaatatga ttgtgtcagg caggcccgta ccccatgcct
     7081 acgaccacca ggccaaacgc gaacccgttc ttgaaaacga atggaccgac ggcagcttca
     7141 aggctgcacg gttcaccctg cgaaaccatg cccgactgac ggcagggcgc aataccgcgc
     7201 atctggacgg cgacataacc gcatacgatc tgtccggcat cgacctcggc tttacccaag
     7261 gcaaaacacc ggaatgctac cgctcctacc atagcggcag cacccactgc acacccaacg
     7321 ccgttttaaa agccgaaaac tatcgtgcac tacctgcaac gcaagtacgc ggcgacatta
     7381 cccttaacga ccgttcagag ctccgcctgg gcaaagcaca cctgtacggc agcatccgtg
     7441 ccggcaaaga caccgcagtc cgcatggaag cagacagcaa ctggacactt tcccagtcca
     7501 gccacaccgg cgcactgacg cttgacggcg cacaaattac cctgaacccc gatttcgcca
     7561 ataatacaca caacaaccgc ttcaacacac tgaccgtcaa cggcacactt gacgggttcg
     7621 gcacattccg attcctgacc ggcatcgtcc gaaaacaaaa tgccccccct caaactggaa
     7681 ggggacagcc gcggcgcatt ccaaatccac gtcaaaaaca ccggacaaga acctcaaaca
     7741 accgaatcgc ttgcacttgt gagcctcaat ccgaaacaca gccaccaagc ccgattcacc
     7801 ctccaaaacg gctatgccga tttgggtgcc taccgctaca tcctccgcaa aaacaacaac
     7861 ggatacagcc tgtacaaccc gctcaaagag gccgaacttc aaattgaagc cacgcgtgcg
     7921 gaacatgagc gcaaccaaca ggcatacaac caattacagg caaccgacat cagcagacag
     7981 gttcaacatg actctgacgc gaccaggcag gcactacagg cctggcagaa cagtcaaacc
     8041 gaacttgccc gcatcgacag ccaagtccaa tatctgtccg cccaattgaa acagacagac
     8101 ccgctgaccg gcattctgac gcgtgcccaa aacctgtgtg ccgcacaagg atacagtgtc
     8161 gatatctgcc gtcaggttgc caaagccgcc gacacgaacg acctgacact cttcgaaacc
     8221 gaactggata cgtatataga acgtgtagaa atggccgaat ccgaacttga caaagcacgg
     8281 caaggcggcg atgcgcaagc cgtcgaaaca gcccggcacg cctacctgaa cgcactcaac
     8341 cgtctgtccc gacaaatcca cagtttgaaa accggcgttg ccggcatccg tatgccgaac
     8401 ctggccgaac tgatcagccg gtcggccaac accgccgttt ccgaacaggc cgcctacaat
     8461 accggccggc aacaggcggg acgccgcatc gaccgccacc ttaccgatcc gcagcagcaa
     8521 aacatctggc tggaaaccgg tacgcaacaa accgactacc atagcggcac acaccgtccc
     8581 taccaacaaa ctaccaacta tgcacatatc ggcatccaaa ccggcatcac cgaccgtctc
     8641 agtgtcggta cgattttaac cgatgagcgc acaaacaacc gttttgatga aggcgtatcc
     8701 gcccgaaacc gcagcaacgg cgcacatctg ttcgtcaaag gggaaaacgg cgcactcttt
     8761 gccgcggcag atttaggcta cagcaacagc cgtacccgat ttaccgatta tgacggggct
     8821 gccgtccgcc gccacgcatg ggatgcaggc atcaacaccg gcatcaaaat cgataccggc
     8881 atcaacctca gaccctatgc cggcatccgt ataaaccgca gcaacggcaa ccggtacgta
     8941 ctcgacggcg cagagataaa cagcccggcg caaatccaaa ccacatggca tgccggcatc
     9001 cgtctcgata aaaccgtcga actgggtcaa gccaagctga cccccgcttt cagcagcgat
     9061 tactaccata cccgccaaaa cagcggttcc gccctcagcg tcaacgaccg taccttactg
     9121 cagcaagccg cccacggcac actgcatacc ctgcaaatcg acgccggata caaaggctgg
     9181 aacgccaaac ttcatgccgc ttacggcaaa gacagcaaca ccgcccgcca caaacaggca
     9241 ggaatcaaaa taggctacaa ctggtaacaa gccggtaaaa atgccgtctg gaacccgcgt
     9301 ttcagacggc atttgcgtta aaaatagtaa accgtttcaa aagggagtag aatagtgccg
     9361 tttccaaccc tgcgcctgta ccgtcaggct tttattatgg accttcccag ttcgttttta
     9421 ctgaacgccc cttccgattc caaacgacaa taaccatccc gccggaatgc ctccccgcac
     9481 acggcgggcg gagcatttat gagcatcgaa cacactcctc cgacacacga cggcgaaacc
     9541 ggtcaaaacc atgccgaacg cccttccgcc gatttcgacc gtgtccactc cctctgcgaa
     9601 atcctcgaac ctgcttttga acaaatcgaa aacggcacac cgctcgaaga cgcgccgctg
     9661 cgcgacaagc tgaccgagct gaccgtcctc ttgggcgagc tgcaccctgc cgacgtggcg
     9721 gcggtgttgg aatcgctgcc gccgcgcgaa cgcaacgtcg tttggcttct ggtcaaaccc
     9781 gaagacgacg gcgaagtatt gctggaagta tccgacgcgg tgcgcgaaac actgatcgag
     9841 tcgatggata aggacgaatt gttggcggca gttgacgatt tggacgcgga cgaattggcg
     9901 gaactggcag acgatttgcc gcaccaagtg gtttacgaag ccttacagac gcgtgatgag
     9961 gaagaacgcg cccaagtcaa ggcggcaatg tcctacgaag acaaccaagt cggtgcgatt
    10021 atggacttcg agttggtcag catccgcgcc gatgtcgcct gcgaagtggt gctgcgttac
    10081 ctgcgccgct tcgacagcct gcccgaccac acggataaaa tcttcgtggt tgacgaaaac
    10141 gatgtgttgc agggcgtgct gcccatccgc aaacttttgg tcgccgatcc cgaagacttg
    10201 gtggaaaacg tgatggcgaa agatgtcgtg cgtttccgcg ccgaagacga cgtggaagaa
    10261 gcagcgcagg cgtttgaacg ctatgacttg gttaccgcgc ccgtcgtcga tgaaaacaaa
    10321 aagctcatcg gcagggtgac cgttgacgaa atggtggacg tgatccgcga agaatccgaa
    10381 gcagatatgt tgaacatggc gggtttgcaa gaagaggaag acttattcgc ccccgtcttg
    10441 gattccgtca aaaaccgctg gatgtggctc gccgtcaacc tctgcaccgc cttccttgcc
    10501 agccgcgtca tcggcgcgtt tgaaggcagc atcgaaaaaa tcgtcgcact cgccgcgctg
    10561 atgcccatcg tcgccggtat cggcggcaac tcgggcaacc agacgattac catgattgtc
    10621 cgcgccatgg cgatggggca actgacaggc atgcaggcag gcaggctgtt gaaaaaagaa
    10681 gtcggcgttg ccctagtcaa cggcattatc tggggaaccg tcatgggcgc ggtttcatgg
    10741 ctgctctacg gcagcctcgg catcgggctg gttatgattg ccgcgatgac gctcaacctc
    10801 ctgctggcgg caaccgtcgg cgtattaatt cccgtggtaa tggaaaagtt cggacgcgat
    10861 cccgcactgg gcagctcggt gctgattacc gccgttaccg actccggcgg cttcctgatt
    10921 ttcttgggtc tcgccaccct gttcctgctt taaatgccgt ctgaacccgc gcaaaaatgc
    10981 cgtctgaagc ggaagctgct tcagacggca tttgactatt tatccttgtt gcacaagatt
    11041 attggacggt atgccggggc agccctttgg caacgccgac cacatcctcc ccgaacagcg
    11101 cgttgacatc ggtttcgtca aacacatatt tgctgagacc tttgcaaaat tcctttccct
    11161 cacgacaacc gaaatcccaa caca
//
LOCUS       DAWHXZ010000047        11068 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000047 DAWHXZ010000000
VERSION     DAWHXZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11068
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.047"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            495..1312
                     /gene="opc"
                     /locus_tag="WIE35_000793"
                     /gene_synonym="opcA"
                     /pseudo
     CDS             495..1312
                     /gene="opc"
                     /locus_tag="WIE35_000793"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
     gene            1475..2119
                     /locus_tag="WIE35_000794"
     CDS             1475..2119
                     /locus_tag="WIE35_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ4153734.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            2138..4048
                     /locus_tag="WIE35_000795"
     CDS             2138..4048
                     /locus_tag="WIE35_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ4153735.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGKIRDLLRRYH
                     RVGHELENGSGEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGGKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENETVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            4069..4434
                     /locus_tag="WIE35_000796"
     CDS             4069..4434
                     /locus_tag="WIE35_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153736.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            4545..6020
                     /locus_tag="WIE35_000797"
     CDS             4545..6020
                     /locus_tag="WIE35_000797"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ4153737.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            6024..6563
                     /locus_tag="WIE35_000798"
     CDS             6024..6563
                     /locus_tag="WIE35_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ4153738.1"
                     /translation="MKNFMSFAKHSDRLPKTVSIQIMKKNREILTVRWSGNTENPKNF
                     SAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANTQT
                     DFSKFKGCLEDADMIILAWGTDRSAYKDEKNRILEFLKAEKFMEKVFCISETGNSSDT
                     RHPSRISYSYQLVQFEESA"
     gene            6560..7696
                     /locus_tag="WIE35_000799"
     CDS             6560..7696
                     /locus_tag="WIE35_000799"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ4153739.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADRHLCAPFDNQTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKSWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTIDACAADQFRQQVRIMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            7743..8225
                     /locus_tag="WIE35_000800"
     CDS             7743..8225
                     /locus_tag="WIE35_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ4153740.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            8331..9386
                     /locus_tag="WIE35_000801"
     CDS             8331..9386
                     /locus_tag="WIE35_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4153741.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGSSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKATNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSIVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            9383..9577
                     /locus_tag="WIE35_000802"
     CDS             9383..9577
                     /locus_tag="WIE35_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153742.1"
                     /translation="MTAMPSEPPFRRHSTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGALHQRGA"
     gene            9968..10573
                     /locus_tag="WIE35_000803"
     CDS             9968..10573
                     /locus_tag="WIE35_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ4153743.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
     gene            10636..10998
                     /gene="ssrA"
                     /locus_tag="WIE35_000804"
     tmRNA           10636..10998
                     /gene="ssrA"
                     /locus_tag="WIE35_000804"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3145 a         3112 c         2418 g         2393 t
ORIGIN      
        1 aaacctgtgt ttgggtttcg gctgtcggga gggaaaggaa ttttgcaaag atctcatcct
       61 gttattttca caaaaacaga aaaccaaaaa cagcaacctg aaattcgtca ttcccgcgca
      121 ggcgggaatc cagaccccca acgcggcagg aatctatcgg aaataaccga aaccggacga
      181 acctagattc ccgctttcgc gggaatgacg gcagagtggt ttcagttgct cccgataaat
      241 gccgccatct caagtctcgt cattccctta aaacagaaaa ccgaaatcag aaacctaaaa
      301 tttcgtcatt cccataaaaa acagaaaacc aagtgagaat aacaattcgt tgtaaacaaa
      361 taactatttg ttaattttta ttaatatatg taaaatcccc cccccgaaag cttaagaata
      421 taattgtaag cgtaacgatt atttacgtta tgttaccata tccgactaca atccaaattt
      481 tggagatttt aactatgaaa aaaacagttt ttacatgtgc catgattgcc ctgaccggta
      541 ctgccgccgc tgcacaagag cttcaaaccg ctaatgagtt taccgtccac accgacctct
      601 cttccatttc ttcaactcgt gctttcctga aagaaaaaca caaagctgcc aaacacatcg
      661 gcgtacgtgc tgatattcct tttgatgcca accaaggcat ccgcttggaa gccggtttcg
      721 ggcgcagcaa aaaaatatta ttaatttgga aacagatgag aacaagctgg gtaagactaa
      781 aaatgtaaaa ctgcccaccg gcgttcctga aaaccgtatc gatctttaca caggctacac
      841 ctacacccaa acgttaagtg attctttaaa tttccgtgtg ggtgccggct tgggttttga
      901 atcttcaaaa gacagcatta aaaccaccaa gcatacgctt cacagcagcc gtcagtcgtg
      961 gtcagccaaa gttcacgcgg atttgctttc ccaactgggt aacggctggt atatcaaccc
     1021 ttggtctgaa gtgaaatttg acctcaagtc ccgctataaa ttaaacaccg gcgttaccag
     1081 tctcaaaaaa gacatcaatc aaaaaaccaa cggctggggc tttggattgg gtgcaaatat
     1141 tggtaaaaaa ctgggcgaat ccgccagcat cgaggcgggg ccgttctaca aacaacgtac
     1201 ttacaaagaa tccggcgagt ttagtgtacc aaccaagagt ggcgacgtat cgctcaccat
     1261 cccgaaaacc agtattcgtg aatacggctt gcgcgtcggc ataaaattct gatgatttga
     1321 aatcatcatt gtcgctttaa atgccaaacc gcaaactatt ttggtttgcg gtttttacgt
     1381 gaaatgaatt tgaatagccg atgccgtctg aaaatttcag acggcatttt tccaatcggt
     1441 taaaatacgc ggtttgtttt tttaggaacg cgccgtgctt gcctccgcca tcgacttcat
     1501 cctccatatc gaccaacacc tgctcgcgct gtcggcgcaa tacggtgtgt ggatttatgc
     1561 gattctgttt ttgattgttt tttgcgaaac cggcctgatt gttacgccct tactgcccgg
     1621 cgattcgctg ctgtttgcgg ccggcggtat tgccgcgctg ggcggtatgg atattcattt
     1681 gatggttgca ttattaagcc ttgccgccat actcggcgac gcgctcaatt ttaccgtcgg
     1741 caaatatttc ggcggcaggc tgttcgccaa tcccgattct aaaatcttcc gacgcgaata
     1801 tctcgataaa acccaccgtt tttacgaaaa acacggcggc aaaacgatta tcatcgcgcg
     1861 cttcgtgccc atcgtccgca ccttcgcccc ctttgtcgcc ggtatgggga aaatgcacta
     1921 tgccaaattt atccgctaca acatcatcgg tggcttgttg tgggttatcc tgttttctta
     1981 cgcgggctat ttctttgcca acttccccgt cgtaaaaaac aatctgggct tggtgatggg
     2041 cggcatcatt attgtttccg tcctgccggg tgcggttgaa attgcccgcg ccaaacttgc
     2101 ggtaaaatcc aaacggtaac tgttgagaat ttgtatcatg aatatattat ctgtagaaaa
     2161 cgcttctttt gccatcggcc acgttgcctt gctcgacaaa acttcttttc aactcgacag
     2221 cggcgaaaaa gtcggcttga tcggacgcaa cggcgcaggc aagtcttcgt ttttaaaaat
     2281 cctcgccggc gtacaaaagc tcgacgacgg gcagattatt gttcaaaaca acctcaaaat
     2341 cgtttatgta ccgcaggaat ccttttttga taaggacgca accgtatttg ataccgttgc
     2401 cgaaggtttg ggtaaaattc gcgatttatt gcgccgttac caccgcgtcg gtcatgagtt
     2461 ggaaaacggt tcgggtgagg ctttgttgaa agaactcaac gaattacaac ttgaaatcga
     2521 agcgaaggac ggctggaagc tggatgcggc agtcaagcag actttgggcg aactcggttt
     2581 gccggaaaac gaaaaaatcg gcaacctttc cggcggtcag aaaaagcgtg tcgccttggc
     2641 gcaggcttgg gtgcagaagc ccgacgtatt gctgctggac gaaccgacca accatttgga
     2701 tattgacgcg attatctggt tggaaaacct gctcaaggcg tttgaaggca gcttggtcgt
     2761 gattacccac gaccgccgtt ttttggataa tatcgctacg cggattgtcg aacttgaccg
     2821 cggcattcta cgttcctatc ccggctcgtt ctctaaatac agtgagaaaa aagcgcaaga
     2881 gttggcagtc gaggcggaac ataaccgctt gtttgacaaa tttcatgctc aggaagaagc
     2941 gtggatacgc aaaggcatcg aagcgcgacg tacccgtaac gaaggacgcg tgcgccgttt
     3001 ggaagagctg cgccgccaac gtgcagaacg ccgcaacgtg cagggacagg tcaactttaa
     3061 gctcgatagc ggtgagaaaa gcggcaaaat catcgccgaa ttggaacacg cttcgtttgc
     3121 ctatggcggc aaagtcatta tggacaaatt ctccgccatc ttgcagcgtg gcgacaaaat
     3181 cggcttaatc ggcccaaacg gtatcggcaa aaccaccttc ctcaagctga ttttgggcga
     3241 attgcagccg acctacggca gaatccgtat cggcagcaag caggaagtcg cctatttcga
     3301 ccagttccgc agcgcgttga atgaaaacga aaccgtgttt tacacgctcg gacagggcaa
     3361 tgattacgtt gaagtcggcg gtaagaaaaa gcatgtgatg agctatttag aagatttcct
     3421 gttcccccct gcccgcgcgc aaagtcctgt ttcttcactc tctggcggcg aacgcaaccg
     3481 ccttttgctg gcaaaactct ttacccgtcc tgctaatatt ttgattttgg acgaaccgac
     3541 caacgacttg gatatcgata cccaagagct gcttgaagat ttgctgcgcg attatcaagg
     3601 cacggtattc cttgtctcac acgaccgtat gttcctcgat aacgtgatta cccaaagcat
     3661 tatttttgaa ggaaatggaa ggttaaaaga atatatcggc ggctatcagg actacatcga
     3721 cgcaaaatca cgcgaagcga agattcagac ggaatccgca cccaaaacag ccgatgccga
     3781 accggtgaaa gaaaaaccca aagccaaccg cacggtcaaa ctgtcctaca aagaacagcg
     3841 cgaactcgac gccctgcccg acgaaatcgc cgccttggaa gccgaacagg cagaaatcaa
     3901 cgcgcagctt tccgatcccg aaatcttcaa agattacgaa aaagccggcg cactgcaaag
     3961 ccgcgccgaa gaaatcgaaa tgctgctttt ggaaaagctg gaacgctggg agctgctgga
     4021 aaccaaacag aacggcaatg ccgtctgaaa tcaaacagga agaaaaaaat gttcaaacga
     4081 cctgaagaaa tcattgtcct gatacttgcc gtcctctgga ttgccggtac gtattttctc
     4141 gccgccctgt ttggagcgga tgcttatacc gtgttaaaaa tcactgccct taccctgctt
     4201 tggtctgccg catcattcct cctttggcaa aaaaacctca gccggcatat ctggccgctg
     4261 ctactcggct gcctgactgc ctgttggtgg ccgtatctga atcaatcggt cgcacccggt
     4321 tctttacctt ggtatgcatc atggacgttc aaaaccatct tagccctgat tcccgtaacc
     4381 gcaggttatc tgtttaaatg gaaacaatac cggcagcgca aaacaaagcc gtaagcccgt
     4441 ccttttcaga cggcatctcc ggtcagactg tcaacaattc cgataaaatg ctatactcgc
     4501 gcccgttgcc gcgccgtccg aaaccgccct ttgggaaagc agaaatgatc agcaaacaag
     4561 aataccaagc ccaagccgcc caaggctaca accgcatccc gctcgtgcaa gaactccttg
     4621 ccgatttgga tacgccgctt tccctctatc tcaaactcgc caaccgcccc tatacctacc
     4681 tgctcgaatc cgttgtcggt ggcgaacgtt tcggccgcta ttcttttatc ggcctgcctt
     4741 gcagccacta tctcaaagcc agcggcaaac acgtcgatgt ttatcaaaac ggcgaaattg
     4801 tcgagcaaca cgacggcaac cccctgccct ttatcgaagc cttccacaac cgcttcaaaa
     4861 cacccgaaat cccaagcctg ccgcgcttta ccggcggact ggtcggctac ttcggttacg
     4921 aaaccatcta caatttcgaa cacttcgccc accgcctgaa aaacaccacc aaagccgacc
     4981 cgctcggcac gcccgacatc ttgctgatgc tgtcgcaaga attggcggtg atcgacaatt
     5041 tgagcggcaa aatccatctc gtcgtttacg ccgatccgtc gcagcccgac ggctacgaac
     5101 gcgcccgcga acgcctcgaa gacatccgca cccagttgcg ccaaagctgc gccatcccgc
     5161 tctcgctcgg cagcaaacac accgaagccg tcagcgaatt cggcgaagag ccgttcaaag
     5221 cctgcgttaa caaaatcaaa gactacatct tcgcaggcga ctgcatgcag gttgtcccca
     5281 gccagcgcat gagcatggaa ttcaccgaca gcccgctcgc cctctaccgc gccctgcgta
     5341 cgctcaaccc ttcgccttat ctcttttact acgatttcgg cgatttccac atcgtcggct
     5401 cctcgcccga aatcctcgtc cgccgcgaac gcgacgatgt catcgtccgc cccatcgccg
     5461 gcacgcgcct gcgcggcaaa acccccgccg aagaccttgc caacgaacaa gatttgttaa
     5521 gcgatgccaa agaaatcgcc gaacacgtca tgctcatcga tttaggacgc aacgacgtcg
     5581 gccgcatcag taaaaccggc gaagtcaaag ttaccgacaa aatggtgatt gaaaaatact
     5641 cccatgtaat gcacatcgtt tccaacgtcg aaggccgtct gaaagacggc atgaccaaca
     5701 tggacatcct cgccgctacc ttccctgccg gcacactctc cggcgcaccc aaagtccgcg
     5761 ccatggaaat catcgaagaa gtcgaaccga gcaaacgcgg catctacggc ggtgccgtcg
     5821 gcgtatgggg tttcaacaac gacatggatt tggcaatcgc catccgcacc gccgtagtaa
     5881 aaaacaacac gctatacgtc caaagcggcg cgggcgtggt tgccgactcc gatccggcct
     5941 ccgaatggca ggaaacacaa aacaaagccc gagccgttat acgcgccgcg cagatggtgc
     6001 aggaagggtt ggataaatga cttatgaaaa actttatgag cttcgccaaa cactcagacc
     6061 gactaccaaa gacggtctct atacagataa tgaaaaaaaa tcgagaaatc ctaacggtta
     6121 gatggagcgg caacacagaa aatcctaaaa atttctcagc tgttgctata ggaattaatc
     6181 caagtaaagc aaatgatgaa agaagcgaca aaacccttac ccaacttgca cgtttcttgg
     6241 atatgtacgg ttttaccaac ttcaaaatgt taaatatttt ttccagttac tctacacagc
     6301 aaacaggaat tagggcaaac acccaaaccg atttttccaa atttaaagga tgtttggaag
     6361 atgctgatat gattattctt gcatggggta cagaccgcag tgcttataag gatgaaaaaa
     6421 acagaatact ggaatttcta aaagcagaaa aatttatgga aaaagtattc tgtatttctg
     6481 aaactggtaa ttccagcgat acaagacatc cgtcaagaat tagttatagc tatcaacttg
     6541 tacaatttga agagtcagca tgaaaaacat atctctttct ccgcccgcca tgctcggcat
     6601 tcttggcggc ggacaattag gcagaatgtt tactgttgct gccaaaacca tgggctacaa
     6661 agtcaccgtt ctcgaccccg atccggacgc gccggcagcg gaatttgccg accgccattt
     6721 gtgtgcgccg tttgacaacc aaaccgcttt ggaagaattg gcaaaatgtg cggctgttac
     6781 gaccgagttc gaaaacgtca atgccgatgc gatgcgtttt ctcgccaaac ataccaatgt
     6841 ttcccccagc ggcgactgcg ttgccatcgc gcaaaaccgc attcaggaaa agtcatggat
     6901 acgcaaagca ggcctgcaaa ccgcgccgta tcaagcaatt tgcaaagccg aagacatcac
     6961 tgaagaaagc atacaatttc tgcccggcat cctgaaaacc gctacattgg gctatgacgg
     7021 caaaggccaa atccgcgtca aaacggtgga tgaactcaaa gccgcgtttg ccgaacaccg
     7081 cggcgtggat tgtgttttgg aaaaaatggt ggacttgcgc ggcgaaattt ccgttatcgt
     7141 atgccgtctg aacaatgaca acgtgcaaac tttcgatcct gccgaaaaca ttcacgaaaa
     7201 cggtatcctc gcctactcca tcgtcccagc ccgactgagt gccgacattc agcaacaggc
     7261 gcgacaaatg gcgcagcgtt tggcggacga attggattat gtcggcgtat tggcggtaga
     7321 aatgtttgtt gtcggtgaca cacatgaatt ggtcgtcaac gaaatcgcgc cgcgtccgca
     7381 caactccggc caccatacga tagatgcctg cgcggcagac cagttccggc aacaggtacg
     7441 cattatgtgc aacctgccgc ctgccgatac caagttgctt tcttcatgct gtatggcgaa
     7501 tattttgggc gacgtttggc aggaagacgg cggcgaaccg gattggtttc ccctgcaaag
     7561 ccggccggac gcgcacctgc acctttacgg taaaaaaacc gcgcacaaag ggcggaaaat
     7621 gggacacttt accgttttaa ccaccgattc ggacaccgca tttcaagaag caaaaaaact
     7681 gcatcagtcc ctataatccg accaacggca cagatgccgt ctgaagccgc cgacatactg
     7741 ccatgaacct gctgacactg ttacgccccg ccaccgtgca agactgcaaa gacatcttca
     7801 aagtccacct gcactccgtc caatacacct gcatcctcag ctacaacgaa cacgccctaa
     7861 aagtatggga aggcttgtta aacacagaaa gctacctgcc gaccatttca gatcccgaca
     7921 aagcactttg ggttgccgaa tacaaaggca acatacaggg atttttccaa atcgactgcc
     7981 aagaagcaca actcgatgcg ctttacgtcc accccctgtt ccacaacctc ggactgggca
     8041 ctgccctgct tcatcaggcg gaaaccatcg cccacaaatc cggtttgagc tttctgaaac
     8101 tctacgcatc gctcaactcc gtcccgtttt accttctcaa ccgctacgaa tcgctcggtt
     8161 cggcagtatt gcagcttgac cccagcatca aaatcaaatg cgaactgatg cggaaacacc
     8221 tctgatccgt ccttataacc aaaactgata atcttcaaat caaacattat ttccacaagc
     8281 gcagacagcc gtataaagtt gccccatcta ccaaccgaca ggacaagcat atgaaaacct
     8341 acgcaccggc actctatacc gcagccctgc tcaccgcctg cagccccgca gccgattcaa
     8401 accatccgtc cggacaaaat gccccggcca ataccgaatc cgacggaaaa aacattaccc
     8461 tgctcaacgc ctcatacgat gtggcacggg atttttacaa agaatacaac cccttattta
     8521 tcaaaacata ccaatccgaa caccccggca catccgtcag catccaacag tcccacggca
     8581 gctccagcaa acaggcatta tccgtagcca acggccttca agccgatgtc gtaaccatga
     8641 accaatcctc cgacatcgac ctgctcgaaa aaaaaggact ggtagaaaaa ggctggcaac
     8701 aagccctccc cgaccacgcc gcgccctaca ccagcactat ggttttcctt gtccgaaaaa
     8761 acaaccccaa acagatccgc gattggaacg accttgccaa agacggcgtt aacatcgtca
     8821 tcgccaatcc caaaacctcg ggcaacggac gctacgcctt cctcggcgca tacggttacg
     8881 gtctgaaagc caccaacggc aacgaacagg aagcccaaaa actcgtcgca tccatcctca
     8941 aaaacacccc cgtttttgaa aacggcggac gcgccgccac caccaccttc acacaacgca
     9001 acatcggcga cgtactcatt acttttgaaa acgaagccaa ctacgtcagc aaaaaactga
     9061 cccaaggaca gtttgaaatc gtctatccga gctacaccat ttccgccgaa agccccgtcg
     9121 ccgtcgtcaa cagcatcgtc gcgaaaaaag gcacgcaaaa aaccgcccgc gcctatctcg
     9181 aatacctttg gagcgaaccg gcgcaagaac tcgccgcctc actctacctg cgcccccgca
     9241 accccgaagt attggcaaga cacaaagccg acttcccaga cttagacaca ttctctcccg
     9301 aagaaaaatt cggcggatgg gacaacatca tgaaaaccta cttcgccgac ggcggcatat
     9361 tcgaccgcct gaccgcacaa aaatgaccgc tatgccgtct gaaccgcctt tcagacggca
     9421 ttccacacat caaaacagaa aaaccgcgcc caaaatcatc atcacaagac acaacggcaa
     9481 accggataca aaacccaaca ccgccgccaa aatgccgtct gaagccgtcg gcgcaaccat
     9541 ccttatctgg ggcgcgcttc atcaaagggg cgcataactt tccttaacca tcccttttga
     9601 cagccaaata aacagtgctt ttgcctgtca gaaacaaaac caatattttg cagttcgtat
     9661 cggaacattt ataaaacatc agatttccgt gtttcgcgtt tccggcaatt caagattggc
     9721 aaaacacgtc agcctgcttc gcaaggcagg tttttaaaac tttatcaggc gttcgggcat
     9781 atcggttcga ccggtccgca tttccggcaa tcaacactgc cgacggttgc cgcatttctt
     9841 tttgatatac aatcacactt tcaaaaaaca tacgcaggca acaaaatcat cctcccccga
     9901 ttggtaaaca ttccggcaaa ttaaaacccc gcccgaaaca gccatcggac aaataacagg
     9961 aaaaaatatg aataccgact tccaaaacat cctcaaacaa ctgggcaaac aagcccaaaa
    10021 agaagcggca gaaaaacagg cagccgaaaa aaacaagcaa aaacaagaac aggatttcga
    10081 tttttcacaa gcagtcggac aagtctcccc attaaaaaac cggcagcaat attatgcgcc
    10141 gcccgacaaa acccccatca aagtccgtcc caaagacaac cgcgccgacg aagaaaacta
    10201 cttctacatc ggcagcacat acaacgaccc gcccgccagc ttcagcaaaa acggacaggg
    10261 aaaaaacgac atccaacgcc tgaaaaacgg atactatccc gtcgttaccg atgtcgacct
    10321 gcacggctac acacaggaag aagcccaaaa agtcctcaac gaattcatcg cattcaccca
    10381 aaaacgcggc gtatgcggcg aaatcatcca cggcagcgga ttaggttcca aaggctacaa
    10441 acccgttctg aaaaatatga cccgaaactg gctgatgcaa caccccgacg tactcgccta
    10501 cgccgaaccc cgggaaggca acgacggctg cgtccgcatc ctgctcaaac gcaaattaag
    10561 gcagcaagac tgacaaattt ctccgacaga cttggcagca ccgcacaata tccgtataat
    10621 ttgcaaacca tttctggggg cgaccttggt ttcgacgagg gttgcgaagc agatgcgggc
    10681 ataccggggt ctcagattcc cgtaaaacac tgaattcaaa tagtcgcaaa cgacgaaact
    10741 tacgctttag ccgcttaagg ctagccgttg cagcagtcgg tcaatgggct gtgtggcgaa
    10801 agccaccgca acgtcatctt acattgactg gtttcctacc gggttacttg gcaggaaata
    10861 agacttaagg taactggttt ccaaaaggcc tgttggtcgg catgatggaa ataagatttt
    10921 caaatagaca caactaagta tgtagaacgc tttgtagagg actttcggac gggggttcga
    10981 ttccccccgc ctccaccata ccctattttc agcagcatcc tgcaacacaa aaaagccttg
    11041 aacttcaaca gtttcaaggc ttttttgt
//
LOCUS       DAWHXZ010000048        10776 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000048 DAWHXZ010000000
VERSION     DAWHXZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.048"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..377
                     /locus_tag="WIE35_000805"
     CDS             <1..377
                     /locus_tag="WIE35_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HEZ4153744.1"
                     /translation="FFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLN
                     SPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDISLQEALKNADSAHD
                     LRLAVQLRSRRAQSSSPDLELL"
     gene            411..1388
                     /locus_tag="WIE35_000806"
     CDS             411..1388
                     /locus_tag="WIE35_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ4153745.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPRVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLDVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRLRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            1392..1742
                     /locus_tag="WIE35_000807"
     CDS             1392..1742
                     /locus_tag="WIE35_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ4153746.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            1747..2526
                     /locus_tag="WIE35_000808"
     CDS             1747..2526
                     /locus_tag="WIE35_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ4153747.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     AEVSAIAEVYEHIYCTIGVHPDSKEAEEFSIAEMVAAAASPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            2552..2766
                     /locus_tag="WIE35_000809"
                     /pseudo
     CDS             2552..2766
                     /locus_tag="WIE35_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
     gene            2867..3178
                     /gene="grxD"
                     /locus_tag="WIE35_000810"
     CDS             2867..3178
                     /gene="grxD"
                     /locus_tag="WIE35_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ4153748.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            3284..3910
                     /gene="upp"
                     /locus_tag="WIE35_000811"
     CDS             3284..3910
                     /gene="upp"
                     /locus_tag="WIE35_000811"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ4153749.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            3933..4253
                     /locus_tag="WIE35_000812"
     CDS             3933..4253
                     /locus_tag="WIE35_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ4153750.1"
                     /translation="MNFQDYLATFSSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            4504..4923
                     /locus_tag="WIE35_000813"
     CDS             4504..4923
                     /locus_tag="WIE35_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153751.1"
                     /translation="MKKMFLSAVLLLSAATRTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFS"
     gene            4944..5705
                     /locus_tag="WIE35_000814"
     CDS             4944..5705
                     /locus_tag="WIE35_000814"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ4153752.1"
                     /translation="METAKPVMLIVRPSGRAAEDVETCLNAGWRAEVLSPVEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKAQIAVGQGSRRALERCLVRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            5720..7054
                     /locus_tag="WIE35_000815"
     CDS             5720..7054
                     /locus_tag="WIE35_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ4153753.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGNNALATCALVLAALGLGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            7051..8268
                     /locus_tag="WIE35_000816"
     CDS             7051..8268
                     /locus_tag="WIE35_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ4153754.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            8343..9407
                     /locus_tag="WIE35_000817"
     CDS             8343..9407
                     /locus_tag="WIE35_000817"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ4153755.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTGLATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVSSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            9585..>10776
                     /gene="radA"
                     /locus_tag="WIE35_000818"
     CDS             9585..>10776
                     /gene="radA"
                     /locus_tag="WIE35_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ4153756.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPLPEKMVAF
                     GEIGL"
BASE COUNT         2662 a         2882 c         2915 g         2317 t
ORIGIN      
        1 actttttccc cgaggagcgg cgcgaacaat tgctgacgga tttgtcgctc aaccttcagg
       61 cgtttatttc gcaacgcctc gttccgcgag acggcggcaa gggcagggtg gcggcagtcg
      121 aggtgctgct caattcgccc ctgatttcgg agttgattca caacggcaac atccatgaaa
      181 tcaaagaagt gatgaaaaaa tccactaccc tgggtatgca gaccttcgat caacaccttt
      241 accaattgta tgaaaaaggc gatatttccc tgcaagaagc attgaaaaat gccgattccg
      301 cacacgattt gcgtttggcg gtacagttgc gcagccgccg cgcgcaaagt tccagccccg
      361 atttggaact gctctgatgg cggtatggat ttccggacgg atggtttgaa atgatttatc
      421 cgtggcataa tgagcaatgg cggcagattg cggaacattg ggagcgtcgt cccaatgcat
      481 ggctgtttgc cggcaaaaaa gatacgggga aaactacatt tgcccgcttt gcggcgaagg
      541 cactgttgtg cgaaacccct gcaccgggct gcaaaccctg tggcgaatgt atgtcctgcc
      601 atctgtttgg acagggaagc catcccgatt tttacgaaat cacccccttg tcggacgaac
      661 ccgaaaacgg acgcaaactg ttgcagatca aaatcgatgc cgtcagggaa atcatcgata
      721 atgtgtacct gacttcggta cggggcggtt tgcgcgtgat tctgattcat cctgcggaaa
      781 gtatgaatgt ccaagccgcc aacagtttgt tgaaagtgtt ggaagagccg ccgccacgag
      841 tggtcttttt gctggtcagc cacgcggcgg acaaggtttt accgaccatt aaaagccgct
      901 gccggaagat ggttttgccc gcgccttccc atgaagaggc attggcatat ctgcgtgaaa
      961 ggggtgtggc ggaacctgag gaacgtctgg ctttccattc cggagcgccg ctgtttgatg
     1021 aggcggacgg tgtccgtgcg ttgcggatta aactgttgga tattttggca gaaccaaggt
     1081 tgttgaagat tttggattac gccgcgcttt tcgataagga gaaacttccg ctcgacgtat
     1141 ttgtcgggtg gatgcagaaa tggctggtcg atttgggatt gtgcctgcaa cacatgaaac
     1201 ccgtctatta tcccgcttat gaagacaggc tgcttcagac ggcatccggt ttccgcctgc
     1261 gcaatgtatt tgcggcggag gatatgctca aacagcttgc cccctacggg tttcatactt
     1321 taaatgtcaa aatgcagatc gagcatctgc tcatcaacta tttggaattg aagaaagaga
     1381 acgggtgaat tatgtcagac ggacaaaata ttccggcaaa aatgatgtcg ttgcagctga
     1441 aagacatgaa tctgctgtac agctcctaca tgccgttttt ggaacacggc ggtctgtttg
     1501 tgcagaccaa cgacgtattt tccatcgggg acgatattct gcttgccgta gaaatcctca
     1561 acttccccaa actgttcctg ccgaccaaag tcgcctggat caatcctgcg cgtacttcct
     1621 ccaaacccaa aggggtgggg ctggcattca caaaacacga aaactgcctg aaagtcaaag
     1681 accagatcga agtcgaactg ggcaacacaa tcggcggcag cagacctacg tttaccatgt
     1741 aacgccatgc atatcatcga ttcgcactgc cacctcaatt ttgaaggttt gaaagaacgc
     1801 ctgcccgaag ttttgtccaa catggaagca aacggcgtgg ggcaggcgct cgccatcagc
     1861 gtcagcaggg aaagttttgc cgaagtatcc gctatcgccg aagtttatga acatatttac
     1921 tgcacaatag gcgtacatcc cgacagcaag gaagccgaag aattttctat tgcggaaatg
     1981 gttgccgcag ccgcctcgcc gaaagtggtc ggcattggcg agacgggttt ggattattac
     2041 tggtgcaaag gcgatttgtc ctggcaacac aaacgctttg cagaccacat cgaagcagcc
     2101 aatcaaaccg gactgcccgt tatcgtccat acgcgtgatg cggcggcgga caccttgtct
     2161 atcctgaaag aatgccgggt taattcgggc gttatccact gtttttccga agacatcggt
     2221 tttgcccgtg cagcaatgga tttggggctt tatatttctt tctcgggaat cgttaccttt
     2281 aaaaacgcac ccttggttca ggaggcggca aaatatgtgc cggacgaccg cattttggtg
     2341 gaaaccgatg cgccgttcct cgcccccgtt cccaaacgag gcaggcagaa cgaaccggct
     2401 tttgtgcgcc ataccgccga acatatcgcc aaattgcgga accaaacatt ggaacaggtt
     2461 gcggcataca cgacggaaaa cttttaccgg ctgtttaaaa aagtacccga tatgcggacc
     2521 gtctgaccct gtgccgacga taaggaaaac catgaaggca attcatccgt atgtatgtcc
     2581 gcgctgctgc cggctgcctg ccaacacgtt tcggacaggc atggcaaatt ccgcttccaa
     2641 attctgcatt gccaaaggcg gcagacggga agtaaaaaag acgaaagcgg cggcggatat
     2701 gccctgtgcc atttgccgga cagcaggatt gtcgaggagt gggaatattt ccgttcacaa
     2761 cattgatact gcgcgatata cggcaaatat tgtgggaagt ttccgctttt gcgtataatg
     2821 cgtcctacct gacaaatttt gtcaacttta tcaaaaggaa taagcgatgg cttccatcca
     2881 cgaccaaatt aaagaagtag taacgacaca ccgcgtcgta ttgtttatga aaggtacgaa
     2941 gcagtttccg caatgcggtt tctcttcccg cgccgtgcaa atcctgaacg cggcgggctg
     3001 caccgattac gttaccgtca acgtattgga aaatcccgaa gtacgccaag gcattaagga
     3061 atacagcgac tggccgacca tcccccaact ttatgtgaac ggcgagtttg tcggcggttc
     3121 ggacatcctg atggaaatgt atgaggcagg cgagctgcaa gagctgctga aagcctgatg
     3181 gattcggcaa tgccgtctga acgtgtttca gacggcattt tcttttccgg caaatcaaaa
     3241 aaaagtataa tggcgcgtct caaaatcaca ctggaacacc gcgatgaacg ttaatgttat
     3301 caaccatccg ctcgtccgcc acaaattaac cctgatgagg gaggcggatt gcagcaccta
     3361 caaattccgg acgcttgcca tcgagctggc gcgcctgatg gcatacgagg caagccgtga
     3421 ttttgaaatc gaaaaatacc ttatcgacgg atggtgcggt cagattgaag gcgatcgcat
     3481 caagggcaaa acattgaccg tcgttcccat cctgcgtgca ggtttgggta tgcttgacgg
     3541 cgtactcgac ctgattccga ctgccaaaat cagcgtagtc ggactgcagc gcgacgagga
     3601 aacgctgaaa cccgtttcct acttcgagaa attcgtggac agcatggacg aacgtcctgc
     3661 cctgattatc gacccgatgc tggcgacggg cggttcgatg gttgccacca tcgacctttt
     3721 gaaagccaag ggctgcaaaa acatcaaggc actggtgctg gttgccgcgc ccgagggtgt
     3781 gaaggcggtc aacgacgcgc accccgacgt tacgatttac accgccgcgc tcgacagcca
     3841 cttgaacgag aacggctaca tcatccccgg cttgggcgat gcgggcgaca agattttcgg
     3901 cacgcgctaa ctgactgatt ttcggatttg atatgaattt ccaagactat ctcgccacat
     3961 tttcttcaat cgaccatctg ggcggtttgg acgttcagga tgccgacggc aaaacggttc
     4021 accacattcc cgccgttcag ggtaagctcg gttcgctcaa gctgtacaat gctttggcag
     4081 aacgttttga cggaaaattg gacaaagaag cggcagaaca gggtttgata tggtttgccg
     4141 aacacgttgc cgacgcgcgc gcccatcccg gcaagcatcc caatatcgac ctgcttgaaa
     4201 aagttgtgca aagcggcgaa accttgctgc tcaagccgct tgccgcgcaa taattttcga
     4261 ccatgccgtc tgaaatccgt ttcagacggc attttgtcgg aaagaagacc gtaaaacggg
     4321 cattttcttt tctatttcag gatacgggca atgatgtttc aacacacagg acgacacata
     4381 aagtgttgcc ctatgtgttg ccctaatttg gaaggggtta cacccctctc aaataacatc
     4441 tgatgctgct gccacgaagg acggatgtcc aaatggcgga gtttcaacca ttaaggaaat
     4501 acgatgaaaa aaatgttcct ttctgccgta ttgcttctgt cggctgcgac ccgaaccgtg
     4561 tgggcggata cggtgttttc ctgtaaaacg gacaacaaca aatatataga agttcaaaaa
     4621 atcaaccgca atctttacga atattcgttc ggtagtgcgg caaaaaaaga aattgccata
     4681 cgcaacagca aagctgacct gttggggcgt tccgacaggt ggcaaggtat gggcagcggt
     4741 cgttgggcaa cgatgaaatt ccaaaacggc gaatttatgt acaccatatg gacaggcttc
     4801 gattccgtga ctcatacgga aagcagcggt gtcgttgtgg agcgtagggg caaggaagtc
     4861 gcacgggtcg gctgtacgcc gaaaaccgcg caggcgaatt tcaacgatga cgatttttcc
     4921 tagtaatcgg ggcggataag gcgatggaaa cagcgaaacc cgtcatgctg attgtccgtc
     4981 cgtcgggcag ggccgcagaa gatgtcgaaa cttgcctgaa tgccggttgg cgcgcggaag
     5041 tattgagtcc ggtcgaaatc gaagcagatg ctgccggact ggaacttttg tccgaacaat
     5101 atgcccgtgc ggatgccgtg ttttgggtca gtccgaccgc cgttgaaacc gccgtcccgt
     5161 accttaacct ttcagacggc ataaaggcgc agattgccgt agggcagggc agccgccgcg
     5221 cattggaacg ctgtttggtc agaacggtca tcgcgcctga tgacggcaac gacagcgagg
     5281 cggttttgcg cctgccggtt tggaacagtc tgcccgaagg tgcgcgcgta ttgtttgtgc
     5341 gcggacacgg cgggcgggat tttttgatga atgccttgca ggagaaaggt tttcggacgg
     5401 aggtggcaga agtctatttc agacggcata aacctttgaa ctttcaaaat ttccaaaccg
     5461 aaaatattgc cgccgcctat attacgtcga ccgagctggt gcgcttgctg ttcgggcagc
     5521 ttccgccgca attttcccga ttcttcaaat ccttgctata ctttacccat catccgcgca
     5581 ttgcggaggc attgaagcgc gaaggcgtgt gttcggtcga aaccgtccct acgctggaag
     5641 ccgcgctttc ccattcttcc atttccgttt cagacggcat ggtctttccc ggaacctcaa
     5701 attaataagg agcaaaacgg tgggcgaacc tgaaaacaaa tcatccgaac ccgtacgcga
     5761 gatacaggca tcaaaagaaa tgccgtctga aacctcttcc ccacgcaaag aaaacgaaac
     5821 agaagtacac attcctgccg ctccttttat cgtcaaacag tccggcaaca acgctttggc
     5881 aacctgcgcc ttggtattgg cggcattggg tttgggcgca agtggttttt tgtttgtcca
     5941 aggacagaat gtcttgaaaa accaagagct ggcattcaac caaaaaatcg acaaagccgc
     6001 cttgggcgag tcggaaaacg ccgccctgct caaagacaac ctcaaccggc aagccgccat
     6061 acaatcagaa ctcgaccgtt tggacggaaa cgtcaaagca aacggcgaac aaatcttgga
     6121 aatgcaaaaa tcctatcgcg agttgaccaa aggacgcgcc gattggctgg tggacgaaac
     6181 cgagaccata ctcaatctgg cggcgcaaca gctggtgttg accggcaata tccaaacggc
     6241 agtcggcgta ttggagcata tcgacagccg cctgtcccgt ttcaatcagg cagagcttct
     6301 gccgatcaaa caggcggtca gcagcgactt ggcggaactg aaaaaccgtc cctatgtcga
     6361 tatttccggc acggcattgc gcctcgacag gctggaaacc gccgtatccg gactgccgct
     6421 gattttggac ggcgtattga agccgggcgt acaggtgaag aacgaagccg cttctgcttc
     6481 atggtggcag aacgtatggg aaaaatccct cggcacattg aaggggctgg tcgaaatccg
     6541 ccgtttggaa aacaacgatg ccatgctgat ttctcccgaa caggcatatt ttgtgcgtga
     6601 aaacctgcgc ctccgccttt tggatgcgcg gactgcatta atgcagcgca acagcgaagt
     6661 ctatcagggc gatttgaaca atgccgaagc cgccgtcaga cagtatttcg atgccaagtc
     6721 tcccgccacg cagtcgtggc tgaaagaact ggcggaattg aaggcgttgg atgtgcgcat
     6781 gacggcggat gacggtttga aaaacagcct aaatgccgtc cgcgcctatc gcgacggtac
     6841 gcgcatgacg gcggcggaaa atcaagaagc agaacaggcg gcttccgaac cggcaaacga
     6901 aaacacagct tccgaaccgg ctgccgcatc ggatgtgaag accatagaag caccgtccct
     6961 gccttcggaa cgcaaaccgg aacagcctgc aaaaaaacag cccgtaccgg aaaaggcagg
     7021 gcgttcgccg tccgctaaag gagaacgcgc atgaaaacgg tagtctggat tgtcgtcctg
     7081 tttgccgccg ccgtcgggct ggcattggct tcgggcattt acaccggcga cgtgtatatc
     7141 gtactcggac agaccatgct cagaatcaac ctgcacgcct ttgtgttagg ttcgctgatt
     7201 gccgtcgtgg tgtggtattt cctgtttaaa ttcattatcg gcgtattcaa tatccccgaa
     7261 aagatgcagc gtttcggttc ggcgcgtaaa ggccgcaagg ccgcgcttgc cttgaacaag
     7321 gcgggtttgg cgtattttga agggcgtttt gaaaaggcgg aactagaagc ctcacgcgtg
     7381 ttggtcaaca aagaggccgg agacaaccgg actttggcat tgatgttggg cgcacatgcc
     7441 gccgggcaga tggaaaacat cgagctgcgc gaccgttatc ttgcggaaat cgccaaactg
     7501 ccggaaaagc agcagctttc ccgttatctt ttgttggcgg aatcggcgtt gaaccggcgc
     7561 gattacgaag cggcggaagc caatcttcat gcggcggcga agatgaatgc caaccttacg
     7621 cgcctcgtgc gtctgcaact tcgttacgct ttcgacaggg gcgacgcgtt gcaggttctg
     7681 gcaaaaaccg aaaaactttc caaggcgggc gcgttgggca aatcggaaat ggaacggtat
     7741 caaaattggg cataccgccg ccagctggcg gatgctgccg atgccgccgc tttgaaaacc
     7801 tgcctgaagc ggattcccga cagcctcaaa aacggggaat tgagcgtatc ggttgcggaa
     7861 aagtacgaac gtttgggact gtatgccgat gcggtcaaat gggtcaaaca gcattatccg
     7921 cacaaccgcc gccccgagct tttggaagcc tttgtcgaaa gcgtgcgctt tttgggcgaa
     7981 cgcgaacagc agaaagccat cgattttgcc gatgcttggc tgaaagaaca gcccgataac
     8041 gcgcttctgc tgatgtatct cggtcggctc gcctacggcc gcaaactttg gggcaaggca
     8101 aaaggctacc ttgaagcgag cattgcatta aagccgagta tttccgcgcg tttggttctg
     8161 gcaaaggttt ttgacgaaac cggagaaccg cagaaggcgg aggcgcagcg caacttggtt
     8221 ttggcaagcg ttgccgagga aaaccgacct tccgccgaaa cccattgaaa ccttcagacg
     8281 gcatcgttga aatgccgtct gaaaaccatt tcacttgaca aacagactga cagaaaaccc
     8341 gtatgactct tttaaaaaac gataccttcc tccgcgccct gcttaaacag cccgtcgaat
     8401 acacgccgat ttggatgatg cgccaggcgg ggcgttatct gcccgaatac aaagccacac
     8461 gcgcgaaagc gggcagcttc ctcgatttgt gcaaaaacac cggattggcg accgaagtta
     8521 ccatccaacc tttggaacgc ttcgatttgg acgcggcgat tttgttctcc gacatcctga
     8581 ccgtcccaga cgcaatgggc ttgggactgt actttgccga aggcgaaggc ccgaaattca
     8641 aacgcgccct gcaacacgaa gccgatatcg ccaagctgca cgttcccgat atggaaaaac
     8701 tgcaatacgt ctttgatgcc gtcagctcca tccgtaaagc actggacggc cgcgtaccgc
     8761 ttatcggctt ctccggcagc ccgttcacgc tcgcctgtta tatggtcgaa ggcggcggca
     8821 gcaaagaatt ccgcaccatc aaaaccatga tgtactcgcg ccccgatttg ctgcacaaaa
     8881 tcctcgatac caacgcccaa gccgttaccg cctacctcaa cgcccaaatc gacgcgggcg
     8941 cgcaggcggt gcagattttc gacacttggg gcggcgtgtt gagcgatgcg gcgtttaaag
     9001 aattcagcct caaatacatc cgccagatcg tcgccggact caaacgcgaa agcgaaggcc
     9061 gccgcgtgcc tgttatcgta tttgccaaag gcggcgggct gtggctggaa agtatggcag
     9121 aaatcggcgc agacgcattg ggcttggact ggacgtgcaa catcggcgaa gcacgccgcc
     9181 gcgtcggcaa gcaagtcgcc ctgcaaggca acttcgaccc gttcgccctc ttcggtacgc
     9241 cggaatccat ccgcaccgag gtcgcacgta tcctagccga ctacggacac ggcagcggcc
     9301 atgtcttcaa cctcggacac ggcatcaacc aacacgccga ccccgaacac gccaaaatct
     9361 tagtcgatac cgtacacgag ctgtctcggc agtatcacgg cgggtaagcc ggcaggaaac
     9421 cgcccgatat gccgtctgaa agccgaggga tggaaaatta ggtaatacgg caatatccgc
     9481 cgtgtacgga tagtacatga cggcggcggt gttgccgcat tggcgcaatc ccaaccgtcc
     9541 ctattttcag acggcatttt tgttttcaga tgcagggaaa accgatggca aaaacgctta
     9601 aaacccttta ccaatgcacc gaatgcggcg gcacctcccc gaaatggcaa ggcaaatgcc
     9661 cgcattgcgg cgagtggaac acacttcagg aaagcttggc cgcgcccgag ccgaaaaacg
     9721 cccgcttcca atcttgggcg gcggatacct cgaccgtcca atccctctcc gccgttaccg
     9781 ccaccgaagt gccgcgcaat ccgaccggta tgggcgaact cgaccgcgta ttgggcggcg
     9841 gtttggtcga tggtgcggtc atcctgctcg gcggcgaccc cggcatcggc aaatccacgc
     9901 tgctgttgca aaccatcgcc aaaatggcgc aaagccgtaa agtgctatac gtttccggcg
     9961 aagaatccgc ccaacaagtc gccctgcgcg cgcagcgttt ggaactgccc accgaaggcg
    10021 tgaacctgct tgccgaaatc cgcatggaag cgattcaagc agcgttgaaa cagcatcagc
    10081 ccgaagtcgt ggttatcgat tccatccaaa ccatgtattc cgaccaaatc acttccgccc
    10141 ccggttctgt gtcgcaggtg cgcgagtgtg ccgcccaact gacgcgcatg gcgaaacaga
    10201 tgggcatcgc catgatactg gtcggacacg tgaccaaaga cggcgcgatt gccggcccgc
    10261 gcgtgctgga acacatggtt gataccgtgc tgtatttcga gggcgaccaa cattccaact
    10321 accgcatgat acgcgccatc aaaaaccgct tcggcgcggc aaacgaactg ggcgtgttcg
    10381 ctatgaccga aaacggtttg aaaggcgtgt ccaacccgtc cgccatcttc cttgccagct
    10441 accgcgacga cacgcccggt tcgtgcgttt tggttacaca ggaaggcagc cgcccgcttt
    10501 tggtcgaaat tcaggcattg gtcgatgacg cacacggctt cacccccaaa cgcctcaccg
    10561 tcggactgga acaaaaccgc cttgcgatgc tgcttgccgt attaaaccgc cacggcggca
    10621 tcgcctgttt cgatcaggat gtgtttttaa acgccgtcgg cggtgtgaaa atcggcgaac
    10681 cggcggcgga tttggcggtc atcctcgcga tgctttccag cttccgcaac cgcccgctgc
    10741 ccgaaaaaat ggttgcattt ggagaaatcg gtttga
//
LOCUS       DAWHXZ010000049        10736 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000049 DAWHXZ010000000
VERSION     DAWHXZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10736
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.049"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..2434
                     /locus_tag="WIE35_000819"
     CDS             <1..2434
                     /locus_tag="WIE35_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ4153757.1"
                     /translation="ENNFIDPAHSKFLFTEKILQGDQVIPIHKVETFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEVELKA
                     NAGDAEFERKGWEHMVLELLLNKNGNPGQNVLLEFTATPPEKADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLSKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     VDESKADYLAFLNWVENVQADDFSFLKTFSMSLNDSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWIKQNYQKHNIIITNSETNKSKTEKTDSETEKLLNNLEAVDNPIRAIFTV
                     GRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSCKTANTTVSEKQLIGRGVRYFPFSFED
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNENFKNLLIWANKKIPNPNAKSNNADSLKANPQTLSFQIHGNQLLQETQ
                     FTADKNDEKVKQLGIQNNFSQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLN
                     IQNRNELQNNLLKDWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDKPFIGT
                     KEFTPKTLWEIFGTPKQKWVKKDDIKTAIAMQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSQYDIHLIRNEEVFKLNNFANGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEKMK"
     gene            complement(2578..3750)
                     /locus_tag="WIE35_000820"
     CDS             complement(2578..3750)
                     /locus_tag="WIE35_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ4153758.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            4041..4487
                     /gene="iscR"
                     /locus_tag="WIE35_000821"
     CDS             4041..4487
                     /gene="iscR"
                     /locus_tag="WIE35_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ4153759.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            4516..5730
                     /locus_tag="WIE35_000822"
     CDS             4516..5730
                     /locus_tag="WIE35_000822"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ4153760.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHSFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESNNLAIKGAAHFYKSKGNHLITVKT
                     EHKAVLDTMRELERQGYEVTYLDVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIIFHVDAAQACGKVPVDVEAAKVDLLSMSGHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYVNGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            5991..6377
                     /gene="iscU"
                     /locus_tag="WIE35_000823"
     CDS             5991..6377
                     /gene="iscU"
                     /locus_tag="WIE35_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ4153761.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            6464..6784
                     /gene="iscA"
                     /locus_tag="WIE35_000824"
     CDS             6464..6784
                     /gene="iscA"
                     /locus_tag="WIE35_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ4153762.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(6744..6965)
                     /locus_tag="WIE35_000825"
     CDS             complement(6744..6965)
                     /locus_tag="WIE35_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ4153763.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            7047..7547
                     /gene="hscB"
                     /locus_tag="WIE35_000826"
     CDS             7047..7547
                     /gene="hscB"
                     /locus_tag="WIE35_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ4153764.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKKSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            7645..10395
                     /gene="gyrA"
                     /locus_tag="WIE35_000827"
     CDS             7645..10395
                     /gene="gyrA"
                     /locus_tag="WIE35_000827"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ4153765.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLAHPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            10552..10736
                     /locus_tag="WIE35_000828"
                     /pseudo
     CDS             10552..10736
                     /locus_tag="WIE35_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3176 a         2801 c         2406 g         2353 t
ORIGIN      
        1 ggaaaataat tttattgacc cggcgcactc aaaattttta tttaccgaga agattttgca
       61 aggcgaccaa gttattccca ttcacaaagt agaaacgttt agcccacatt cagacggcat
      121 tgaaattaaa tttaccagta ttcaaaaact gtataacgat atccacaccg aacgggaaaa
      181 ccaaaccaca ttggcagatt tgcacgaatt gaaccttgtg atgctgggcg atgaagcgca
      241 ccatttaaac gcacaaacca aaggcaaaaa acaaggcgag ttggatttgg aagtggaatt
      301 aaaagccaat gcaggcgatg ccgaatttga acgcaaaggc tgggagcata tggttttgga
      361 attgttgctc aataaaaatg gcaatcccgg ccaaaatgtg ctgttggaat ttaccgccac
      421 gccacctgaa aaagctgacg tgcaacaaaa atacgctgat aaaatcatca caaaatttgg
      481 tttaaaagaa tttttgcaaa aaggttatac caaagaaatc aatttggtat ccagtacact
      541 gagcaagaaa gagcgggtgt tgcacgcttt attgtttgct tggtatcgcc atcaaatcgc
      601 tttgaaatat ggcattgcca atttcaagcc tgtgatgttg tttagaagta agacggttga
      661 tgaatcaaaa gcggattatt tggcattttt aaattgggta gaaaatgtgc aagcagatga
      721 tttttcgttt ttaaagacat tttcaatgag cttaaacgat agcgataacg ccaacgaaca
      781 aggcaaaacc cgcaccgaac aagccctaaa atttatgcag gaaaatggct ttgagtttgc
      841 acatttagca aattggataa aacaaaatta tcaaaaacac aatatcatta ttaccaactc
      901 cgaaaccaac aaaagcaaaa ccgaaaaaac cgacagcgaa acggaaaaat tgctaaacaa
      961 tctggaagcg gttgataatc cgattcgtgc tatttttacg gtgggtagat taaccgaagg
     1021 ttgggacgtt ttaaatttgt ttgatattgt gcgtttgtat gaagggcaaa acggcggcgg
     1081 ttcaaataaa aaatcatgca aaacggcaaa tacaacggta tcagaaaaac agttgattgg
     1141 tcgaggcgtg cgttattttc cattttcgtt tgaagataaa cagccgaata agcgtaaatt
     1201 tgacaacgac atgcaacacg aattgcgtat tttggaagaa ttgttttatt acacgcacga
     1261 tgagcaatcc cgctatatta cagaactgaa aaacgagtta cgaaaagacg gttatttgcc
     1321 tgaaaaagac gatgataagg tattggcaac atttaaactc aaatctgaat ttgcagataa
     1381 tgaaaatttt aaaaacttat tgatttgggc gaataaaaaa atccccaatc ccaatgctaa
     1441 aagtaataat gcagacagcc taaaagccaa tccgcaaaca ctttcattcc aaattcacgg
     1501 caatcaactg ttgcaggaaa cgcaatttac agccgataaa aatgatgaaa aggtcaagca
     1561 acttggcata caaaataatt tttctcaaac cataaaaatg agtgaaatgg aacggcacat
     1621 cttcaataaa gcattacata tcaaagggaa aaatagtcaa tctttattcc atttcaaccg
     1681 tttgcaaagc aaactcaaca ttcaaaatcg caatgaattg caaaataact tgttgaaaga
     1741 ctggcaaatt gaatttttag gattagagca agataaacag gttcgcccgg atgataaact
     1801 tgcaggctgc ctgaaaatct tggaaatggt tgaaaagcat ttgaatgaaa gtgataagcc
     1861 atttatcggt acaaaagaat ttacgcctaa aacattatgg gaaattttcg gcacgccaaa
     1921 acaaaaatgg gtcaaaaaag atgatataaa aactgctatt gccatgcaaa atgattggta
     1981 tgtgatggat aattttgccg gaacgggttt ggaagaagcg ttaattcaat ttatttcagc
     2041 gcgtttgggc gatttgaaat ctcaatatga cattcattta atccgtaacg aagaagtgtt
     2101 taaattgaat aactttgcca atggcgaagg atttatgccc gactttattt tattgctgaa
     2161 agataaacaa aaatcttctt ccaatggcgt gaatgacttt ttgcattacc aaattttcat
     2221 tgagcccaaa ggtgagcatt tagtggaaac tgatggatgg aaagaagaat ttttagaagc
     2281 catcacagtg gaatatggga tggataagat cctgcaaaaa gatacaccgc attatcgttt
     2341 gatcggttta ccgtttttta ctgatcatca gaaaaatgga caatttacgg agtcattccc
     2401 tttaggtgcg gcatcgcttg aaaaaatgaa gtagtgcatt gcaggcagcc ccgtttgaca
     2461 aaacttcctt tacaaaaggg cgttttgtca gatatttaat caacacatta ttaaaataca
     2521 gccaaatttt aatgccgtct gaaccctgtg ttcagacggc atcgtatttt tcagtatcta
     2581 aaccgtttcc ctaccccaat ccttacccct caaaatcgag gcatcgacat cttgaatatc
     2641 gcggtgtccc gtaaacgcca tagatatatc catttcttta tacaggattt ccagcgcacg
     2701 ggttacgcct tcttcgccat acgcacccaa gccatacaga aacgcccgac ctatcattgt
     2761 acctttcgca cccaaagccc acgccttcaa aatatcctga ccgctgcgga tgccgctgtc
     2821 catccaaact tcgatgtcgc tgccgactgc actaacgatg tcgggcaagg ctttgatggc
     2881 agacacggta tcgtcgagct gtcggccgcc gtggttggaa acaatcaatg cgtccgcgcc
     2941 gcttttcgct gctttttccg cgtcttcggg ttccataatg cctttgataa tcagcttgcc
     3001 gccccacaaa tctttaatgc gcgccacatc gtcccagctc aggcgcgggt cgaattgttc
     3061 ggaagtccat gaagacagcg aagacaaatc gccgacgttc ttcgcgtgtc cgacgatatt
     3121 gcggaacgtg cggcgttccg tgttcagcat tttcatgcac cattccggtt tggtcgccag
     3181 attgattaaa ttggcgatgg tcggtttcgg cggcgcggac aggccgtttt tgatgtcttt
     3241 gtggcgttga cccaaaacct gcaaatcggc ggtcaatacc aatgccgaac atttggcatc
     3301 cttcgcgcgc ttaatcaggt tttccataaa ctcgcggtcg cgcatcacat aaagctgaaa
     3361 ccaaaatggt gcggaagtgt tctcggcaac gtcttcaatc gagcagatag acatcgtgga
     3421 cagcgtaaac ggaatgccga acttctccgc cgcccgcgcc gccaaaattt caccgtcggc
     3481 gtgtgccata cccgtgaaac ccgtcggcgc aatcgccacc ggcattttca catcctgccc
     3541 gatcattttg gtttccaggc ttcggccttc catattgacc aatacctttt gtcggaagcg
     3601 gatgtctttg aaatccgaag tgttttcacg gtaggtagtt tctgtccacg aacccgaatc
     3661 gatgtaatcg taaaacatac gcggcatttt gcgcttggca acgcggcgca agtcttcgat
     3721 gcaggtcatt ttgctcaaat cacgtttcat ttgtcgcccc ctgaatacct gaataacttt
     3781 atatgaaatc gataatgtat atcaatattg attataaggc aaatcatttc aacatttgcc
     3841 gcatccgccg cagctcccta ctttaagcga cataaggttt aaaattcaaa aataacaaat
     3901 taaaatcaaa atattaaaaa tcaatcaatc tatcgattta aacagccaat cacacaatcc
     3961 gcccccatac ttgactgaaa cactcagata ttggacaatt ccacccacta ataaaaaaac
     4021 cgacatgggc aaccaccacc atgagactga ccaccaaagg gcgtttcgcc gttaccgcta
     4081 tgctggattt ggcgatgaac gcgcaaaccg gcgccgtcaa actcagtgcc atcagcgaac
     4141 gccaaaacat atccctctcc tatctcgagc aattgttcgg caaactccgc cgcgccggac
     4201 ttgttgaaag cctgcgcggg cccggcggcg gctacatcct cgccgcaccg gcggcacgca
     4261 tcaacatcgc ccaaatcatc gccgccgccg aagaccggct ggacgcaacc caatgcggca
     4321 gcaaagccaa ctgccaccac ggcgcgccct gcctgacgca cgatctttgg gagaatttaa
     4381 acaaaaccat caacgactac ctcggcagcg ttaccctgca aagcatcatc gaacagaaaa
     4441 acaacggcga cggcagccgc gtcgtccaat ttacacacat ccattaaata acacccgaaa
     4501 aagaaagagc aaaccatgac cgtcaaaacc cccgtttacc tcgactacgc cgccaccacc
     4561 cccgttgaca aacgcgttgc cgaaaaaatg attccctatc tgaccgaaac cttcggcaac
     4621 ccagcctcca acagccacag cttcggctgg gaagcagaag aagctgtaga aaaagcacgt
     4681 gcagacattg ccgccctgat taacgccgac tctaaagaaa tcgttttcac cagcggcgca
     4741 accgagtcca acaacctcgc tatcaaaggc gcggcgcact tctacaaatc taaaggtaat
     4801 cacctcatca ctgtaaaaac cgaacacaaa gccgtactcg acaccatgcg cgaactcgaa
     4861 cgccaaggtt acgaagtaac ttatctggac gtacaagaaa acggtttggt tgatttagac
     4921 gtactgaaag ccgccatccg cgaagacacc atcctcgttt ccgtaatgtg ggtaaacaac
     4981 gaaatcggcg tggttcaaga tattcctgcc atcggcgaaa tctgccgcga acgcaaaatc
     5041 attttccacg ttgacgcagc acaagcatgc ggcaaagtgc ctgttgatgt tgaagccgca
     5101 aaagttgatt tgctgtctat gtccggccac aaagtatacg gccctaaagg catcggcgcg
     5161 ctgtacgtcc gccgcaaacc ccgcgtccgc ctcgaagccc aaatgcacgg cggcggccac
     5221 gaacgcggct tccgcagcgg cacattgccg acccaccaaa tcgtcggtat gggcgaagcc
     5281 ttccgcatcg ccaaagaaga actcgaacaa gatatggcgc actaccgcaa actgcgcgac
     5341 atcttcctca aaggcatcga aggcatcgaa gaagtctatg tcaacggcga cctcgaacac
     5401 cgcgccccga acaacctgaa cgtcagcttc aacttcgtcg aaggcgaaag cctgattatg
     5461 gcggtgaaag aactcgccgt atccagcggc tccgcctgta cttctgccag tctcgaaccc
     5521 agctacgtcc tgcgcgcgct cggccgcaac gatgaactgg cgcactcatc cctgcgcatc
     5581 accttcggtc gcatgaccac tgaagaagaa gtgcaatttg ccgccgaact gattaaatcc
     5641 aaaatcggca aactgcgcga actgtcgccg ttgtgggaaa tgttcaaaga cgggattgat
     5701 ttgaactcga ttgaatgggc ggcgcattaa agcgtaccaa catgccatcc gaaccttcag
     5761 acggcattcc aaaaacaaag caatcaagag aaaaatatga acgaacaaga tttagatttg
     5821 gacaacctcg acaacctgct tgacgatttc gacggcgtta ccgtggaagg cggcgtcgat
     5881 tcggaaaacg acgacggctg tgaaggcgga gcgtgcaaaa tctaactcag gccgtctgaa
     5941 aaccggcaaa caaaccactt aaatcatcaa aacattaagg aaaccacatc atggcataca
     6001 gcgataaagt aatcgaccac tacgaaaatc cccgcaacgt cggtactttc gacaaaaacg
     6061 acgaatctgt cggcaccggc atggtcggcg caccggcctg cggcgacgtg atgcgcctgc
     6121 aaatcaaagt gaacgatgaa ggcatcatcg aagatgcgaa attcaaaact tacggctgcg
     6181 gttccgccat cgcttcgtcc agcctgatta ccgagtgggt taaaggcaaa agcctggatg
     6241 acgcgctggc aatcaaaaac agcgaaatcg ccgaagagtt ggaattgccg ccggtaaaaa
     6301 tccactgctc catcttggct gaagatgcgg taaaagcggc cgttgccgac taccgcaaac
     6361 gtcaggaaaa cagataaagc ccttcagacg gcatcgtccc gcaatgccgt ccgaaccgcc
     6421 cgccgcttcg ggttcgtccg aggcggtaca acaaggaaga aatatgatta cccttaccga
     6481 gaatgccgca aaacacatca atgactatct cgccaaacgc ggcaaaggct tgggcgtacg
     6541 cttgggtgtg aaaaccagcg gctgctcggg gatggcgtac aaccttgaat ttgtcgatga
     6601 agccgatggc gacgacctga ttttcgaagg acacggcgca cgcatttata tcgacccgaa
     6661 aagcctggtt tatctggacg gcacacaagt cgattacacc aaagaaggtt tgcaggaagg
     6721 tttcaaattt gaaaacccca atgtcaaaga ctcctgcggc tgcggcgaaa gcttccatgt
     6781 ttaagacata aaaatggcgg gaccgtatca aaaccgtccc gccatttttt cgcttcctgc
     6841 ctgttgtagc tgcctttgcc ttttcttttc cgttccacct tgtgtcggaa caaatcagat
     6901 ttcactaagg cttttaaagc attgtcgcgt attttgcctt tattgtgctg cactttgccg
     6961 cccatattca gtcctttcgt ttaagaagcg gcagattata aggcaaaaac agttttctgc
     7021 caaaatctta catttatcat cctactatgt cccaatattt caccctcttc cggattgaac
     7081 ccgctttcga tatcgacacc gaaaacttgg aacaaaccta ccgcgccttg gccgcccgtt
     7141 tccatcccga taaattcgct tcagcttccg cctttgagca aaagcaggca gtgatgatgt
     7201 cttccaccat caacgatgcc taccgcacct tgaaaaaccc catcgaccgc gccgcctacc
     7261 tgctgaaaaa atcgggcatc gatgccgacg cgccggagca taccgctttc gcccccgaat
     7321 tccttatgca gcaaatggaa tggcgcgaaa cgctgatgga ggcacgggca ggcaacgacc
     7381 ttgaatcctt gaaaaatctc gacaacgaaa tccgcgacga gcaagaaaaa ctgttctgcg
     7441 gtctgaaaca gtcgtttgcc cgacaagatt acgacacagc cgcacaacaa gtccgccaag
     7501 gcaggtttct cgacaaactc cgcaacgaaa tttcctcggc attataatcc gcaccgtgtt
     7561 tcagacggcg taaccgccgc accgttccgc gtcaaaatat gctaaaataa gcaacaattt
     7621 tttgccatac gaaacattga aaccatgacc gacgcaacca tccgccacga ccacaaattc
     7681 gccctcgaaa ccctgccggt aagccttgaa gacgaaatgc gcaaaagcta tctcgactac
     7741 gccatgagcg tcattgtcgg gcgcgcgctg ccggacgttc gcgacggtct caagccggta
     7801 caccgccgcg tactgtacgc gatgcacgag ctgaaaaaca actggaatgc cgcctacaaa
     7861 aaatcggcgc gcattgtcgg cgacgtcatc ggtaaatacc acccccacgg cgataccgcc
     7921 gtatacgaca ccatcgtccg tatggcgcaa aatttcgcta tgcgttatgt gctgatagac
     7981 ggacagggca acttcggatc ggtggacggg cttgccgccg cagccatgcg ctacaccgaa
     8041 atccgcatgg cgaaaatttc ccacgaaatg ctggcagaca ttgaggaaga aaccgtcaat
     8101 ttcggcccga actacgacgg tagcgaacac gagccgcttg tactgccgac ccgtttcccc
     8161 acactgctcg tcaacggctc gtccggcatc gccgtcggca tggcgaccaa tatcccgccg
     8221 cacaaccttt ccgataccgt caatgcctgc ctgcgcctgc tcgatgcacc cgacaccgaa
     8281 atcgacgaac tgatcgacat tatccaagcc cccgacttcc cgaccggggc aaccatctac
     8341 ggcttgagcg gcgtgcgcga aggctataaa acaggccgcg gccgcgtcgt tatgcgcggt
     8401 aagacccata tcgaacccat aggcagaaac ggcgaacgcg aagccatcgt tatcgacgaa
     8461 atcccctatc aggtcaacaa agccaagctg gtcgagaaaa tcggcgattt ggttcgagaa
     8521 aaaacactgg aaggcatttc cgagctccgc gacgaatccg acaaatccgg tatgcgcgtc
     8581 gttatcgagc tgaaacgcaa cgaaaatgcc gaagtcgtct taaaccaact ctacaaactg
     8641 actccgctgc aagacagttt cggcatcaat atggtggttt tggtcgacgg acaaccgcgc
     8701 ctgttgaacc tgaaacagat tctctccgaa ttcctgcgcc accgccgcga agtcgttacc
     8761 cgacgtacgc ttttccggct gaagaaggca cgccatgaag ggcatatcgc cgaaggcaaa
     8821 gcggtcgcac tgtccaatat cgatgaaatc atcaagctca tcaaagaatc gcccaacgca
     8881 gccgaggcca aagacaaact gcttgcgcac ccttggcgca gcagcctcgt tgaagaaatg
     8941 ctgacgcgtt ccggtctgga tttggaaatg atgcgtccgg aaggattggc tgcaaacatc
     9001 ggcttgaaag agcaaggtta ttacctgagc gagattcagg cagatgctat tttacgcatg
     9061 agcctgcgaa acctgaccgg cctcgatcaa gaagaaattg tcgaaagcta caaaaacctg
     9121 atgggtaaaa tcatcgactt tgtggatatc ctctccaaac ccgaacgcat tacccaaatc
     9181 atccgcgacg aactggaaga aatcaaaacc aactatggcg acgaacgccg cagcgaaatc
     9241 aacccgttcg gtggcgacat tgccgatgaa gacctgattc cgcaacgcga aatggtcgtt
     9301 accctgacac atggcggcta tatcaaaacc cagccgacca ccgactatca ggcgcagcgt
     9361 cgcggcgggc gcggcaaaca ggcggctgcc accaaagacg aagactttat cgaaaccctg
     9421 tttgttgcca acacgcatga ttatttgatg tgctttacca atttgggcaa gtgtcattgg
     9481 attaaggttt acaaactgcc cgaaggcgga cgcaacagcc gcggccgtcc gattaacaac
     9541 gtcatccagt tggaagaagg cgaaaaagtc agcgcgattc tggcagtacg cgagttcccc
     9601 gaagaccaat acgtcttctt cgccaccgcg cagggaatgg tgaaaaaagt ccaactttcc
     9661 gcctttaaaa acgtccgcgc ccaaggcatt aaagccatcg cgctcaaaga aggcgactac
     9721 ctcgtcggcg ctgcgcaaac aggcggtgcg gacgacatca tgctgttctc caacttaggt
     9781 aaagccatcc gcttcaacga atactgggaa aaatccggca acgacgaagc ggaagatgcc
     9841 gacatcgaaa ccgaaatttc agacggcatc gaagatgaaa ccgccgacag cgaaaacgca
     9901 ctgccgagcg gcaaacacgg tgttcgcccg tccggtcgcg gcagcggcgg tttgcgcggt
     9961 atgcgcctgc ctgccgacgg caaaatcgtc agcctgatta ccttcgcccc tgaaaccgaa
    10021 gaaagcggtt tgcaagtttt aaccgccacc gccaacggat acggaaaacg caccccgatt
    10081 gccgattaca gccgcaaaaa caaaggcggg caaggcaata ttgccattaa caccggcgag
    10141 cgaaacggcg atttggtcgc cgcaaccttg gtcggcgaaa ccgacgattt gatgctgatt
    10201 accagcggcg gcgtacttat ccgcaccaaa gtcgaacaaa tccgcgaaac cggccgcgcc
    10261 gcagcaggcg tgaaactgat taacttggac gaaggcgaaa ccttggtatc gctggaacgt
    10321 gttgccgaag acgaatccga actctccgac gcttctgtaa tttccaatgt aaccgaaccg
    10381 gaagtcgaga actgaaaatc atctcccgat gccgtctgaa gattcagacg gcatttattt
    10441 tatccctcat ccgtcatcca gcttctcaca atatagcgga ttatagtcaa ttaaaaacaa
    10501 ggggctgtcc tagataacta gggaaattca aattaagtta gagttgcccc tatgagaaaa
    10561 agtcgtctaa gccggtataa acaaataaac tcattgaact gtttgtcgca ggtgtaactg
    10621 caagaacgac agcagagtta gtaggcgtta ataaaagtac cgcagcctat tattttcatc
    10681 gtttacgatt acttatttat caaaacagtc cgcatttgga aatgtttgat ggcgaa
//
LOCUS       DAWHXZ010000050        10623 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000050 DAWHXZ010000000
VERSION     DAWHXZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.050"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(119..1336)
                     /locus_tag="WIE35_000829"
     CDS             complement(119..1336)
                     /locus_tag="WIE35_000829"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ4153766.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLDAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAVS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1538..2266)
                     /gene="rph"
                     /locus_tag="WIE35_000830"
     CDS             complement(1538..2266)
                     /gene="rph"
                     /locus_tag="WIE35_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ4153767.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2348..2812)
                     /locus_tag="WIE35_000831"
     CDS             complement(2348..2812)
                     /locus_tag="WIE35_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ4153768.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3029..3805
                     /locus_tag="WIE35_000832"
     CDS             3029..3805
                     /locus_tag="WIE35_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ4153769.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPECQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            3943..4470
                     /locus_tag="WIE35_000833"
     CDS             3943..4470
                     /locus_tag="WIE35_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153770.1"
                     /translation="MEFVNNLVIFSFLLLMLIPIFFVVYGIYHKIRYRKIYILRISFT
                     LLVVILCSMYYIYCRYLDQQKVAYYCIDEQCISIVHLYKDYGINSPTYARIYAGKILF
                     RFQVRAKNYAELLMEDDISISKKILGNKFIIYGSLPVIYGNVDNIEVKEATGYIDRAS
                     TDYIVSRNLKFRHLY"
     gene            4641..5495
                     /locus_tag="WIE35_000834"
     CDS             4641..5495
                     /locus_tag="WIE35_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ4153771.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5536..6744
                     /gene="pncB"
                     /locus_tag="WIE35_000835"
     CDS             5536..6744
                     /gene="pncB"
                     /locus_tag="WIE35_000835"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ4153772.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEVELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6836..8554
                     /gene="argS"
                     /locus_tag="WIE35_000836"
     CDS             6836..8554
                     /gene="argS"
                     /locus_tag="WIE35_000836"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ4153773.1"
                     /translation="MNLHQTVEREAAAAFAAAGIADSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVSEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKTGEWDADAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            9105..10523
                     /locus_tag="WIE35_000837"
     CDS             9105..10523
                     /locus_tag="WIE35_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ4153774.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIVGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVVAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGKM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHSKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2532 a         3016 c         2613 g         2462 t
ORIGIN      
        1 ttaatccact atattttttg ttccaaagtc aaaatatgcc gtccgaacat tcgggcggca
       61 gacaaaacgg cactgcccga taaaggcagt gccgttgtcc gtttcaaacc gtgaaacatc
      121 agcccaaatt aaaggcttta tgcaataccc tggttgccag ttccatgtat ttttcatcaa
      181 tcaatacgga aactttgatt tcggaggtgg aaatcatttg gatgttgata ccctcttcgg
      241 cgagcgtgcg gaagattttg gcggctacac cgacgtgcga acgcataccc aaaccgactg
      301 cagagacttt gcatacggtg tcgtcgccat caatagaaac cgcgccgata ctgtcttggc
      361 gttccgacag gatttccaaa gtctgcttgt aatcgccgcg cggtacggta aaggaaaaat
      421 cggttgtgcc ttcgctgccg acattttgga taatcatatc gacttcgatg ttggcatcgg
      481 caaccgcgcc taaaatctga taggcgacac cgggtttgtc gggtacgccg cgcacgttga
      541 tgcgggcttg gtttttatcg aatgcgatac cggttacggc agctctttcc atgttgtcgt
      601 cctcttcaaa ggtaattaag gtgccattgc cgccgtcttg caggctgctc agtacgcgca
      661 ggcgcacttt gtattttccg gcgaattcta ctgaacggat ttgcaaaact ttcgaaccga
      721 ggcttgccag ttcgatcatt tcttcaaatg taaccgtatc catgcggcgc gcttcgggta
      781 cgacgcgggg gtcggttgtg taaacgccgt ctacgtcggt atagatttgg cactcgtcgg
      841 ctttgagggc tgcggcaagc gcaacggcgg aagtgtcgga accgccgcgt ccgagcgtgg
      901 aaatatcgcc ttcactgctg atgccttgga agccggcaac gatgacgact ttgcctgcat
      961 cgaggtcggc acgcattttt tcgtcatcaa tgctttcgat gcgggctttg gtgtgggcgg
     1021 tatcggtttt gagggcgacc tgccagcctg tgtagctttt ggcatccacg ccgatgtctt
     1081 tcaatgccat cgccaaaagg ccgatggtta cttgttcgcc ggtggacaaa accacgtcca
     1141 attcgcgcgg atcgggatgc tcctgcattt cgtgcgccaa cgcgaccagg cggttggttt
     1201 caccgctcat ggcggatacg acgacgacga tgtcgtgtcc ttcggcgcgg gctttggcga
     1261 cacgtttggc cacgttttta atgcgttcgg gcgagcctac ggatgtgccg ccgtatttgt
     1321 gtacgattaa cgccatgttt cgtgctttct tgtgtgggat tggtcgggca gcatggtttg
     1381 ctggaaaagc ggctattatt actatttttt acatgaagtt caagaacgga ctgcgctttc
     1441 ccgcctgccg tttgacagcg gtcagcgaaa aacctgttct ttcagattgt tgacaaaatg
     1501 ccgtctgaac ggttttcaga cggcatccgg acgacaatca ggcggcggac aacgcatttt
     1561 gctggtgttg cagcagttcg cctatgcctt tttgcgccag tgcaaccagt ttgcccaatt
     1621 cgtccaaact gaacggcgcg tcttccgccg tcccctgtat ttcgatgatt tttcccgatg
     1681 cggtcatgac gatattcaca tcactgtcgc aaccggagtc ttcgggataa tccaaatcca
     1741 aaagcggcac gccgttcact acgcctactg acacagcggc aacggcttcg cggatggggt
     1801 tttcactcaa aatgccgtct gaaaccagtt tgccgacggc gatttgcagc gcgacaaacg
     1861 caccggtaat cgaagccgtg cgcgtaccgc catctgcctg aatcacatcg cagtcaatca
     1921 agatttgtcg ttcgccgagt ttttccatat ccacgaccgc gcgcagcgaa cgcccgataa
     1981 ggcgttggat ttcttgtgtg cgcccggact gtttgcccgc cgaagcttcg cggcgcatcc
     2041 gggaagcagt tgaggcaggc agcatcccgt attccgccgt tacccagcct tggtttttac
     2101 cgcgcagaaa cggcgggacg ttttcatcta tggaagcggt acaaatcact ttggtattgc
     2161 cgcattcaat aaggcacgaa ccgtccgtat gcggcaggaa atgaggggtg attttgatat
     2221 cgcgcaggct gtccgcggcg cgcgagatgc ggatgtaatc aggcatactg ccctcccgtt
     2281 aaaaacagat aaattaaaaa gccttaaata tgaaaaatca catttaaggc cttcaaactg
     2341 aaaatttcta cgcctcttcg gctttgctgc ggataatcaa aagcggcagg tggctttggc
     2401 gcattaccgt ttcggcaaaa ctgcccatta aaaggtgcat cagtccggta cgtccgtgcg
     2461 tgcccaacac cagcaggtcg gcaccgtttt catcggcata atcaaccaaa tcctgcgcca
     2521 tttcacgcgc acccttattg gcaaccagca ggtgtttgac ggtattttcc acacccagtt
     2581 cccgggcggt gcgctcggcg gcatccaaaa cttcgttgcc ttgcgcgacg gcggcggctt
     2641 cgtagctttc gtgttgcaaa aattcggggg cgagcgccat atattcggca ggattggcaa
     2701 cgtgtaccaa agtcagacgc gcaccgttga ccccggcaag ctcggcggca tgtttcaggg
     2761 cattgatgga cgtttcactg ccgtcaacgg caacaaccaa atgtttgtac atatcgtatt
     2821 ctccttttgc accgcctcgc ggtgccctct tgtcggatgg gcgcagggac agtttgcgct
     2881 gtttcattat agacccgccg tcgggcttta tacaacagcc gaacagcccg accgctttcc
     2941 agtataatat gccgcttccg tgcagtcagg cattttttgc cggctttcgt tcactttttg
     3001 atttgacgca atcttgcagg attcgaccat gtccgacaac gctttgacct cttcgcgacg
     3061 cttcggcggc atcgccagac tctacggaga ctctgccttg gcgcactttt cacaggcaca
     3121 cgtctgcgta gtcggcgtgg gcggtgtcgg ctcgtgggcg gtcgaggctt tggcgcggac
     3181 gggcatcgga cgtttgactt tgattgattt ggacaacgtt gccgaatcga atgtcaaccg
     3241 ccagctgcac gccctgaccg gagacttcgg caaagcaaaa gttaccgcct tgcgcgaacg
     3301 cgtcgcccaa atcaacccag aatgccaagt gtttgaaatt gaagatttcg ttaccgaaga
     3361 caatttgccg gaatacttcg gaaaaggttt tgatttcgtc atcgacgcga tcgaccaagt
     3421 gcgcgtcaaa gcagcaatgg cggcttattt tgtggaacgc aaacaaccgt ttgtcctcag
     3481 cggcggcgcg ggcggacaaa aaaatccggc gttaatccaa accgccgatt tgagccgcgt
     3541 aacccacgac ccgctgcttg ccaacctgcg ctacaccttg cggaaacgct acggattcag
     3601 ccgcgatacg aaagcaaata tgcgcgtgcc ttgcgtgtat tcgaccgaaa atatcgtgcc
     3661 gccgcagtct agggaggctt gttcggcaga tgccgctccg caaggcttgt cgtgcgccgg
     3721 ctacggtgca agcatgctcg ttaccgcttc gttcgggcta tattgcgcac aggcggcggt
     3781 ggaacacatc gcagacaaaa aataagcaat gccgtctgaa acaggattca gacggcattt
     3841 gaacaaacta tggttatgat ttaagacaac aaaggatacg gataaaaaat aacataaaat
     3901 atatgattcc taataatata ccaagtatcg gagagctatt taatggaatt cgttaataat
     3961 ttagttattt tttcattttt attactaatg cttattccga tattttttgt agtatatggt
     4021 atataccata agatacgtta tcgcaaaata tatatcctaa gaataagttt tacattatta
     4081 gtggtaatac tttgcagtat gtattacata tattgccgtt atcttgacca acaaaaagta
     4141 gcttattatt gcatagatga acaatgtatt tctattgttc atctatacaa agattatggt
     4201 ataaactctc ccacatatgc gagaatttac gcaggaaaaa tattgtttag atttcaagta
     4261 agagctaaaa attacgctga attacttatg gaagatgata tatcaattag taaaaaaatt
     4321 ttggggaata aatttatcat ttatgggtcg ctacctgtaa tatacggtaa tgtagataat
     4381 attgaagtaa aagaagctac tggttatata gatagagcca gtactgatta tattgtctca
     4441 agaaacttaa aattcagaca tttatattaa ttaagaggtt ttagcaagac cgtattcaca
     4501 acctgctcct tttcaaaaca tttgcattta aaagccgtta taatgccgtc tgaacatctg
     4561 cccgaccaca ttatgcgtga atgccggcag attgttttct tttgtaaact tatattaaaa
     4621 tccacttacc gattcacgcc atgccgccca tccctgcccc atccgcacca tccgagcaca
     4681 ctgtcgcatg ggtattcggc caacccgtta ccgatttgcc ccaggatttg tttattccgc
     4741 ccgatgcatt gaaagtcgta ttgggcagtt ttcaaggccc tttggatcta ctgctgtatc
     4801 tgatccgcaa gcagaacatc gatgttctcg atattccgat ggtgaagatt accgagcagt
     4861 atctgcacta catcgcccaa atagaaacct atcagtttga tttggcggcg gaatatcttt
     4921 tgatggcagc aatgctgatt gaaatcaaat cgcgcctgct gctgccgcgt accgaagccg
     4981 tcgaagacga agaagccgac ccgcgtgccg agttggtgcg ccgcctgttg gcttatgaac
     5041 agatgaagct ggcggcacaa gggttggatg cgcttcctcg tgcgggccgg gatttcgcgt
     5101 gggcgtacct gcctctggaa attgccgtcg aagccaagct gcccgaagtc tatattaccg
     5161 acttgacgca agcgtggctg ggtattttgt ctcgggcaaa acacacgcgc agccacgaag
     5221 taatcaaaga aaccatctcc gtgcgcgcgc aaatgacggc aatcctgcgc cgtttgaacg
     5281 gacacgggat atgcaggttt cacgacctgt tcaatcccga acagggcgcg gcttacgtgg
     5341 tcgtcaactt catcgcactg ttggagcttg ccaaagaagg tttggtcgga atcgtacagg
     5401 aagtcggttt cggagaaatc cgaatcagcc tcaatcatga gggggcacat tcagacggca
     5461 tttccggcac acggggcggg cgcgatgtgt tctaatacgc cccaagccgc caccaaaaat
     5521 cgggagacac gccatatgac cggcatcata cattcgctgc ttgacaccga cctctacaaa
     5581 ttcactatgc tgcaagtagt cctgcaccag tttccgcaga cgcacagcct ttacgaattc
     5641 cgctgccgca acgcctcgac cgtctatccg cttgccgaca tcaaagaaga cttggaagtc
     5701 gaactcgacg cgctctgccg gctgcgcttc acccacgacg aactcggcta tctgcgctcc
     5761 ctgcgtttca tcaaaagcga ctttgtcgat tatctcgaac tcttccagct ccaacgccgc
     5821 tttgtcgaag tcggcacaga cgataaaggc cgtctgaaca tccgcatcga aggtccgatg
     5881 atacaggcga tgttctttga aatcttcatc ctcgccattg tcaacgaact ttacttccgc
     5941 cgcctggaaa cccctgcagt catagaagaa ggcgaacgcc gccttcaagc caaagccgcg
     6001 cgcctcaaag aaatcgccgc cgcacaaaac cccgacgaac cgcccttcct gatttccgac
     6061 ttcggcacgc gccgccgcta caagctcgcg tggcaggaac acgtcatccg caccctgctc
     6121 gaagccgccc caagcatcgt gcgcggcacc agcaatgtct ttctcgccaa aaaactcggc
     6181 atcaccccca tcggcaccat ggcgcacgaa ttcctccaag ccttccaggc cctcgacgta
     6241 cgcctgcgga atttccaaaa ggccgcgctc gaaagctggg tgcacgaata ccggggcgat
     6301 ttgggcgttg ccctgaccga cgtggtcggt atggatgcct tcctgcgcga tttcgacctc
     6361 tatttcgcca aacttttcga cgggctgcgc cacgacagcg gcgaccctta cgtttggggc
     6421 gacaaagcct acgcccacta tcaaaagctc aaaatcgaca gccgcaccaa aatgctgacc
     6481 ttctccgacg ggctggacat cgaacgctct tgggcattac accaatattt caaagaccgc
     6541 ttcaaaaccg gcttcggcat cggcaccaac ctcaccaacg atatggggca tacgcctttg
     6601 aatatcgtgt tgaaactggt cgaatgcaac gggcagtccg tcgccaagct gtccgactct
     6661 ccgggcaaaa ccatgaccaa caacagcacc ttcctcgcct acctgcgcca agtgttcgac
     6721 gtacccgaac ccgaaacgcc gtaaaccggc agaaaaagcg cacaattcct gtttctgccg
     6781 cataaaatct tttaaaatac cgcctgattt gaatttaacc gaaagaccga acttcatgaa
     6841 cctacatcaa accgtcgaac gcgaagccgc cgccgccttt gccgccgcag gcatcgccga
     6901 cagtcccgtt gttttgcagc cgaccaaaaa tgccgaacac ggcgatttcc aaatcaacgg
     6961 cgtgatgggt gcggcgaaaa aagccaaaca aaacccgcgc gaactggcgc aaaaagtcgc
     7021 cgacgcattg gcaggcaacg ccgtcatcga aagcgcggaa gtcgccggtc cgggctttat
     7081 caacctgcgc ctgcgccccg aatttctcgc gcaaaacatt cagacggcct tgaacgacgc
     7141 acgtttcggc gtggcaaaaa ccgacaaacc gcaaaccgtc gttatcgact attcttcgcc
     7201 caatctggca aaagaaatgc acgtcggcca tctgcgttcc agcatcatcg gcgacagcat
     7261 ttcgcgcgtg tcagaattta tgggcaatac cgttatccgt caaaaccacg tcggcgactg
     7321 gggtacgcag tttggcatgt tggtcgctta tttggtcgag cagcaaaaag acaatgccgc
     7381 attcgagctg gcggatttgg agcagtttta ccgcgccgcc aaagtgcgct ttgacgaaga
     7441 ccccgccttt gccgacaccg cgcgcgaata cgttgtgaag ctgcaaggcg gcgatgaaac
     7501 cgttttggca ttgtggaaac agtttgtcga tatttcgctc tcgcacgccc aagccgttta
     7561 cgacacgctg ggcttgaagc tgcgccccga agacgtggcg ggcgaatcga aatacaacga
     7621 cgatttgcag cccgtagtcg atgatttggt tcaaaaaggt ctggcagtcg aagacgacgg
     7681 cgcaaaagtc gtgttcttgg acgagtttaa aaacaaagag ggcgaacctg ccgcatttat
     7741 cgtgcaaaaa caaggcggcg gcttccttta tgcttccacc gacttggcgt gtttacgcta
     7801 ccgtgtcggc acgcttcatg ccgaccgcct gctgtacgtc gtcgaccacc gccaagccct
     7861 gcactttgag caactcttta ccacttcacg caaagcaggc tatctgccgg aaaacgtcgg
     7921 cgcggcattt gtcggcttcg gcacgatgat gggcaaagac ggcaaaccgt tcaaaacacg
     7981 cagcggcgac actgtaaaac tggtcgatct gctgaccgaa gccgtcgaac gcgccaccgc
     8041 tttggtgaaa gagaaaaatc ccgaattggg cgcggacgaa gccgccaaaa tcggcaaaac
     8101 cgtcggcatc ggcgcggtca aatacgccga cttgagcaaa aaccgcacca gcgattatgt
     8161 gttcgactgg gacgctatgc tctcgtttga aggcaacacc gccccctatc tgcaatacgc
     8221 ctacacccgc gtgcaaagcg tgttccgcaa aacaggcgaa tgggatgcgg acgcgccaac
     8281 cgttttgacc gaaccgctgg aaaaacagct tgccgccgag ctgctgaaat ttgaagacgt
     8341 actgcaaagc gtggcggaca cggcgtatcc gcactacctc gccgcctacc tctatcaaat
     8401 tgcgaccctg ttcagccgct tctacgaagc ctgtccgata ctcaaagccg aaggcgcaag
     8461 ccgcaacagc cgcctgcaac tggcaaaact caccggcgac acgctgaaac aaggcttgga
     8521 tttgctgggc atcgatgtgt tggacgtaat gtaaaaccgc accgcccgat tgcggacaac
     8581 agcctcgcca tccttatccg aatctgaaaa aagcggcgcg atacaccgta tccgccgccc
     8641 ctcccaaaat gcgaaacaaa caaacgccaa gcaagcaagc aagcaagcaa gcaagcaagc
     8701 aagcaagcaa gcaaaaaatt ataaccccct tcctgccgac gcacgcactt tccgcgcggc
     8761 gcattcccct ttttccgccc ctcaaacccg ccttttcttc aggcagggtt tcagcccgcc
     8821 ccctttccct gttttccttt ccccgacacg cgcgcgcgac ccctgccgca tcgtgccgca
     8881 ctttcgcgcc cggacggcat cgttccgcca tccggttctc tgtttcaaat acccctgttt
     8941 cagaaagaaa ttctagatgc ttcaacacac aggacgacat ataaagcgtc gccctatatg
     9001 ttgccctgat ttggaagggg ttacgcctcc caaataaagt ctgatcctgc cgccccgaag
     9061 gacagatgtc cgagtggcga agtttcaacc gaaaaggaaa tacgatgaat attcacaccc
     9121 tgctctccaa acaatggacg ctgccgccat tcctgccgaa acggctgctg ctgtccctgc
     9181 tgatactgct tgcccccaat gcggtgtttt gggttttggc actgctgacc gccaccgccc
     9241 gcccgattgt caatttggac taccttcccg ccgcgctgct gatcgccctg ccttggcgtt
     9301 tcgtcaaaat tgtcggcgta ttggcgtttt ggctggcggt tttgtttgac gggctgatga
     9361 tggtgatcca gcttttccct tttatggacc tcatcggcgc catcaacctc gtccccttca
     9421 tcctgaccgc ccccgcccct tatcagataa tgaccgggct gttgctgctg tatatgctgg
     9481 cgatgccgtt tgtgttgcag aaagccgccg ccaaaaccga cttccggcac attgccgtct
     9541 gcgccgccgt cgtggtggca gccggctatt tcaccggcca tttgagttac tacgaccggg
     9601 ggcggatggc caatatcttc ggcgcaaaca acttctatta cgccaaaagt caggcgatgc
     9661 tctacaccgt cagccagaat gccgacttta ttaccgccgg cctggtcgat cccgtcttcc
     9721 tccccttggg caatcagcag cgtgccgcca cgcatctgaa cgagccgaaa tctcaaaaaa
     9781 tcctctttat cgtcgccgaa tcttgggggc tgccggccaa tcccgagctt caaaacgcca
     9841 cttttgccaa actgctggcg caaaaagacc gtttttcggt ttgggaaagc ggcagttttc
     9901 cctttatcgg cgcgacggtc gaaggcaaaa tgcgcgaact gtgcgcctac ggcggtttgc
     9961 gcgggtttgc actgcgccgc gcgcccgacg aaaaatttgc ccgctgcctc cccaaccgtt
    10021 tgaaacaaga aggttacgcc acctttgcga tgcacggcgc gggcagttcg ctttacgacc
    10081 gcttcagctg gtatccgagg gcgggctttc aagaaatcaa aaccgccgaa aacctgatcg
    10141 gtaaaaaaac ctgcgccatt ttcggcggcg tgtgcgacag cgagctgttc ggcgaagtgt
    10201 cggcattttt caaaaaacac agcaaaggat tgttttactg gatgacgctg accagccacg
    10261 ccgactatcc cgaatccgac attttcaacc acaggctcaa atgcaccgaa tacggcctgc
    10321 ccgccgaaac cgacctctgc cgcaacttca gcctgcacac ccaatttttc gaccaactgg
    10381 cggatttaat ccaacgcccc gaaatgaaag gcacggaagt catcatcgtc ggcgaccatc
    10441 cgccgcccgt cggcaacctc aatgaaacct tccgctatct caaacagggg cacgtcgcct
    10501 ggctgcactt caaaatcaaa taacaacaat gccgtctgaa cgcaccaaca gccttcagac
    10561 ggcattttgc agacagaccg acccctcaag cccacttttt catcatttcc gataaattgc
    10621 ttt
//
LOCUS       DAWHXZ010000051        10584 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000051 DAWHXZ010000000
VERSION     DAWHXZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10584
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.051"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(164..1168)
                     /gene="gap"
                     /locus_tag="WIE35_000838"
     CDS             complement(164..1168)
                     /gene="gap"
                     /locus_tag="WIE35_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ4153775.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPSGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTSDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            1612..4206
                     /gene="mutS"
                     /locus_tag="WIE35_000839"
     CDS             1612..4206
                     /gene="mutS"
                     /locus_tag="WIE35_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ4153776.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQMDGVPIKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCTTHMGSRLLALWLHHPLRNRAHIRARQEAVTALDSQYEPL
                     QCRLKNIADIERIAARIAVGNARPRDLAALRDSLFALSEIDLSTNGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAALNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            complement(4329..4820)
                     /locus_tag="WIE35_000840"
     CDS             complement(4329..4820)
                     /locus_tag="WIE35_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ4153777.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDFGTLLGSAAVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(4824..5219)
                     /locus_tag="WIE35_000841"
     CDS             complement(4824..5219)
                     /locus_tag="WIE35_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153778.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDYAWNAVYGAGGTKASGN"
     gene            complement(5240..6634)
                     /locus_tag="WIE35_000842"
     CDS             complement(5240..6634)
                     /locus_tag="WIE35_000842"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ4153779.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNAGNVVYQTRRFDRYKEVIAELLEKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            complement(6750..7406)
                     /locus_tag="WIE35_000843"
     CDS             complement(6750..7406)
                     /locus_tag="WIE35_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ4153780.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFTSSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            complement(7403..7756)
                     /gene="arsC"
                     /locus_tag="WIE35_000844"
     CDS             complement(7403..7756)
                     /gene="arsC"
                     /locus_tag="WIE35_000844"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ4153781.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNNALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            7880..8362
                     /locus_tag="WIE35_000845"
     CDS             7880..8362
                     /locus_tag="WIE35_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ4153782.1"
                     /translation="MKYLNLAAITLGATFAAHTASADELAGWKDGAAQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            8370..8561
                     /locus_tag="WIE35_000846"
     CDS             8370..8561
                     /locus_tag="WIE35_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153783.1"
                     /translation="MPSESASDGIFPARPSVSAKLIHYLKTGGIFIIGTVLPIVQTAY
                     PCGRNRPKRYAAHSLQDLL"
     gene            8558..9208
                     /gene="ftsE"
                     /locus_tag="WIE35_000847"
     CDS             8558..9208
                     /gene="ftsE"
                     /locus_tag="WIE35_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ4153784.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            9205..10122
                     /gene="ftsX"
                     /locus_tag="WIE35_000848"
     CDS             9205..10122
                     /gene="ftsX"
                     /locus_tag="WIE35_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ4153785.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITIYMETAAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPEEIV
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            10212..10460
                     /locus_tag="WIE35_000849"
     CDS             10212..10460
                     /locus_tag="WIE35_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ4153786.1"
                     /translation="MLTPEQVKAMIEDVAKCEHIKVEGDGHHFFAVIVSSEFEGKARL
                     TRHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
BASE COUNT         2582 a         3045 c         2553 g         2404 t
ORIGIN      
        1 tttaaatcag gacaaggcaa cgaagccgca gacagtacaa atagtacggc aaggcgaggt
       61 aacgccgtac tggtttaaat ttaatccact atacagataa acaatgccgt ctgaacgcaa
      121 tgtgttcaga cggcatttac ttatccacag gtttgttcaa gccttagatt ttgcctgcaa
      181 agtattccaa agtgcggacg agttggcagg tgtaggacat ttcgttgtcg taccaggcaa
      241 cggttttcac caattgtttg ccgcccacgg tcatcacgcg ggtttgggtc gcatcgaaga
      301 gcgagccgta ttcgatgccg acaacgtcgg aagaaacgat ttgatcttcg ttgtagccgt
      361 aagattcgct ggcggctgct ttcatcgcgg cgttgatttc ttctttggtt acagggcgtt
      421 cgaggacgga aaccaattcg gtcagcgagc cggtggcaac agggacgcgt tgggcggagc
      481 cgtcgagttt gccgttcaat tcagggataa ccagaccgat ggccttggcg gcgccggtgc
      541 tgttgggcac gatgttgagc gcggcggcgc gggcgcggcg caaatcgcct ttgcggtgcg
      601 gcgcgtcaag ggtgttttgg tcgctggtgt aggcgtggat ggtggtcatc aggccttcga
      661 ctacgccgaa ttccttttgc aggactgccg ccatcggggc aaggcagttg gtggtgcagg
      721 aagcagcgga gataacggtt tcgctgccgt ccaaaatgtc ttggttcacg ccatatacga
      781 cggttttcac atcattgccg ctgggtgcgg aaatcacgac tttgcgcgcg ccggccctga
      841 tgtgtgcttc ggctttggtt ttattggtaa agaagccggt acattcgagg acgacatcca
      901 cacccaactc gccccaaggc aattcttcgg gattcggatt ggcgaaaact ttgatttctt
      961 tgccgtttac cacgatggca tcgtctttta attcggcagt accttggaaa cggccttgcg
     1021 tgctgtcgta tttgaaaagg tgcagcagca tttcggcagg ggtcaggtcg ttgacagcga
     1081 cgacttcgat gccgtgggct ttttcaattt gacgcaatgc gaggcggccg atgcggccga
     1141 aaccgttaat cgctacttta atgctcatgt atatactcca agctgtgaaa cgaaatttca
     1201 atatctgtat tgtattctga aataaagtta cattccacta ttacatctaa ctacttgatg
     1261 cttatttgat atagatgaat tttactgttt gcacagattt ccaaaacttt taccatcaat
     1321 atttgaattt aaaattttaa tgatgatttt gatgattgcg gatctgcttg tgtataagtt
     1381 gcaaatatcc aatattttca ttaccttttc gtcaaataag tttgagttta aggcttgccg
     1441 tataggacag ataaacgtgg atgttttttg acttaataat attcctgtgg ataactttgc
     1501 tgttttccta cttgtctcca caactttatt gacaggctta cggtcagtct cattccgtcg
     1561 aagacaaaac cttttgctac aataccgttt tcctaatgat aaggcagccc catgtccaaa
     1621 tccgccgttt ccccaatgat gcagcaatac ctcggcatca aagcgcaaca taccgacaaa
     1681 ctggtgtttt accgtatggg cgatttttac gagatgtttt tcgacgatgc ggtagaagcg
     1741 gcaaaacttt tggatattac cctgaccacg cgcgggcaga tggacggcgt accgattaaa
     1801 atggcaggcg tgccgtttca cgccgccgaa caatatctgg cgcgcctggt caagttgggc
     1861 aaaagcgtgg cgatttgcga acaggtcggc gaagtcggcg caggcaaagg gccggtggaa
     1921 cgcaaagtcg tgcgcatcgt aacgcctggc acgctaaccg attccgcatt gctggaagac
     1981 aaggaaacca accgcatcgt tgccgtgtcc cccgacaaaa aatacatcgg tttggcgtgg
     2041 gcatcgctgc aaagcggcga attcaaaacc aagctgacga ctgtggataa attggacgac
     2101 gaactggcgc gcctgcaggc ggcggaaatt ctgttgcctg acagtaaaaa cgcaccgcaa
     2161 cttcagacgg catcgggtgt tacgcgcctg aacgcgtggc agtttgccgc cgacgcgggg
     2221 gaaaaactgc tgacggaata tttcggctgc caggatttgc gcggcttcgg tttggatggc
     2281 aaagaacacg ccgttgcgat tggcgcggca ggtgcactgt tgaactatat ccgcctgacg
     2341 caaaacctga tgccgcaaca cttggacggc ctgtcgctcg aaaccgacag ccaatatatc
     2401 ggtatggatg ccgccacgcg ccgcaatctc gaaatcacgc aaaccctctc cggcaaaaaa
     2461 tcgccgaccc tgatgtccac gctcgacctt tgcaccaccc acatgggcag ccgcctcttg
     2521 gctctctggc tgcaccaccc tttgcgcaac cgcgcccaca tccgcgcccg ccaagaagct
     2581 gtaaccgcgc tggacagcca atacgaaccc ctccaatgcc gtctgaaaaa cattgcggac
     2641 attgaacgca ttgctgcccg catcgccgtc ggcaacgccc gcccgcgcga cctcgccgcc
     2701 ctgcgcgaca gcctgtttgc cctgtcagaa atcgacttgt ccacaaacgg cagcagcctg
     2761 ttggaaaccc tcaaagccgt tttcccggaa aacctatcca cagccgaaca gctccgccaa
     2821 gccattttgc ccgaaccttc cgtctggcta aaagacggca atgtcatcaa ccacggtttt
     2881 catcccgaac tggacgaatt gcgccgcatt caaaaccatg gcgacgaatt tttgctggat
     2941 ttggaagcca aggaacgcga acgtaccggt ttgtccacac tcaaagtcga gttcaaccgc
     3001 gttcacggct tttacattga attgtccaaa acccaagccg aacaagcacc tgccgactac
     3061 caacgccggc aaaccctcaa aaacgccgaa cgcttcatca cgccggaact gaaagccttt
     3121 gaagacaaag tactgactgc tcaagagcaa gccctcgcct tagaaaaaca actctttgac
     3181 ggcgtattga aaaaccttca gacggcattg ccgcagcttc aaaaagccgc caaagccgcc
     3241 gccgcgctgg acgtattgtc cacattttca gccttggcaa aagagcggaa cttcgtccgc
     3301 cccgagtttg ccgactatcc ggttatccac atcgaaaacg gccgccatcc cgttgtcgaa
     3361 cagcaggtac gccacttcac cgccaaccac accgaccttg accacaaaca ccgcctcatg
     3421 ctgctcaccg gccccaatat gggcggcaaa tccacctaca tgcgccaagt cgcgctgatt
     3481 gttttattgg cacacaccgg ctgtttcgtg cctgccgatg ccgccacaat cgggcccatc
     3541 gatcaaatct tcacccgcat cggcgcatcg gacgacctcg cctccaaccg ctccaccttc
     3601 atggtcgaaa tgagcgaaac tgcctacatc ctgcatcacg ccaccgaaca aagccttgtt
     3661 ttaatggacg aagtcggacg tggcacttcc actttcgacg gcctcgccct cgcgcacgcc
     3721 gttgccgaac acctgctgca aaaaaacaaa tccttcagcc tgtttgccac ccactatttc
     3781 gagctgacct acctgcccga agcccacgcc gccgcactca atatgcacct ttccgcgctc
     3841 gaacagggac aggacatcgt gtttctgcac caaatccaac ccggtcccgc cggaaaaagc
     3901 tacggcatcg ccgttgccaa actcgccggc ctgcctgtac gcgcattgaa atccgcccaa
     3961 aagcatttga acggactgga aaaccaagcc gccgcgaacc gtccccaact ggatattttc
     4021 agtaccatgc cgtctgaaaa aggagatgaa ccgaatgtgg gcaactttgt ggataaagca
     4081 gaggaaaaac attttgaagg tatattggca gcagccttgg aaaaactcga tcccgacagc
     4141 ctgaccccgc gcgaagcatt gtcagaactg taccgtctga aagatttgtg caaatccgta
     4201 tcttaatttc cgttgtcgga acagcatcaa accatatgga aaaatctgtg gataaacatt
     4261 atctgacaag aagtttccaa acataaaaat gccgtccgaa cagctcagac ggcatttgtc
     4321 cattcggctt aaaccttatc catatccaaa cgcataaccg taacccattc accgttatgg
     4381 aaatgtcgcc cgacaaccgc ccagccgaat gattcataaa atatttgcac atcaggcgta
     4441 taaagataca agaactttat ccccagcgaa cgcgctgcgc ctatgcagtg ggcgaccagc
     4501 ctcctgccaa tgccttttcc gcgatattca ggtaaaacaa agacatcacc caaccaatat
     4561 tcatactgtg gaaaactttc catatcatgc cgcttgaccg cagccgaacc caacagggtt
     4621 ccgaaatcat ccacagccgc aaatgccagc ggcagttcgt catccttcaa acacctgccg
     4681 taataggcat gaatcttatc cacagaagac cacggttcaa atccgtgcca ctcctcaaac
     4741 aacgcctgaa ccaacctgcc gatatgcccg gctttcagcc gtgtaatgaa aacagtattg
     4801 tccacaaaga gggaattcat cggtcaattc cccgacgctt tcgttccccc tgcgccgtaa
     4861 accgcattcc aagcatagtc caaacgcact ccgatttgcc tcagctcttc agcctgccgg
     4921 gctttttgcg ccattgctgc aggaatttcc gcttccaaac gggcgatgtc tgcttgagcc
     4981 gtctgcaaac gccggcgcgc atcttccaaa tccgactgca tcccgatgat ttttccgtcc
     5041 agattgtttt gtttttgcag taaggcacga tagccggatt ggatgctgag cagattgtct
     5101 tccgcatccc cggcccatac gcttgtagaa aaaacaaaca tcagaaaata caatattttt
     5161 ttcatgttca acttccgttt gaatgctgtc tgaagccgca ttccgacatc agacggcatc
     5221 gcccacgcct gtggataact taagcgcgga tgcgtttcaa cacttcttct ttgccgatta
     5281 atgccaacac agcatcgacg ctgggggttt tcgccgtacc gcagacggca aggcgcaggg
     5341 gcatgccgag tttgcccatt ttaatgcctt cttcgtcgca gaagggtttg aagaggtcgt
     5401 ggatggcttc ggcattccag tcttccagcc cttcgaggcg ttcggcaaag cgcagcatac
     5461 gggcggcggc ttcatcgtcc cagtgtttct gcacgtccgc ttcagtaggc gtttgtttga
     5521 cgtagaagta gaggcactcg tcggcaagtg tgttcaggtc ttgggcgcgg tctttgacca
     5581 gtgccaacac gtcttccaat gcaggttttt cagtttcatg gatatcgcgc agagcgagac
     5641 gcggtttgac gagttcggcg agtttgccgt tgggcgtgat tttgatgtgt tcgccgttga
     5701 tccagtagag tttcttcaaa tccatgcggc ttggagatgg agaaacgtct ttcaaatcaa
     5761 accattcgat aaattgttcc atcgtgaaga actcatcgtc gccgtgcgcc cagcccaaac
     5821 gtgccagata gttgagcatg gcttcgggca ggatgcccat cgcgccgaaa tcggtaatgg
     5881 caacggtatc gccgctgcgt ttggagattt ttttgccttg ttcgttaaga atcatcggca
     5941 ggtggccgta ttcaggcagg gtagcgccga tggcttttaa gatgttgatt tgtttcgggg
     6001 tgttgttcac atggtcgtcg ccccggataa cgtgggtaac gcccatgtcg tagtcgtcca
     6061 cgacaacgca gaagttgtag gtcggcgtgc cgtcggcgcg ggcgataatc aggtcgtcga
     6121 gtgcttcgtt ggggatggag atttcgcctt tgaccaagtc cgtccacttg gttacgccat
     6181 ccaaaggcgt tttgaagcgg acgacgggtt gcacgccggc agggatttcg ggcagggttt
     6241 tgcctgcttc cggccgccag cggcggtcgt aagtcgccgt gccttctttt tcggctttct
     6301 cgcgcatggc ttccaactct tctttgctgc aatagcagta gtaggcatcg cctttttcta
     6361 aaagttcggc gatgacttct ttatagcggt cgaaacggcg ggtttggtac acgacgttgc
     6421 cggcgttgtc gtaatcgagt ccgacccatt tcatgccgtc gaggatgatg ttgacggatt
     6481 cggcagtaga gcgcgccaag tcggtgtctt cgatgcgcaa taagaattcg cctttatgat
     6541 ggcgggcaaa cgcccaagaa aacagggcgg tgcgtacgcc gccgatgtgc aggtagccgg
     6601 ttgggctggg ggcgaaacgg gttttgacgg tcatgatggc tccaaaatct ttaaaacggc
     6661 ttattttact gtttttaccg tgcttgggca ttaaaaatgc cgtccgaacc ctgcctgcgg
     6721 ataagtttca gacggcattt tctaccgctt caatcagaca gccatcccaa aaagcggctg
     6781 ccccattttt ccaaacggta gagggataac gcatatccct cttgcagcat aaagattttt
     6841 ttcttatttc ccgcatcaaa ccgcgtggtc ggggtggcag acatataaac gcggacaccc
     6901 aaatcctccg ccatttccgc cgcccgcgcc aaatggtagg gatcgctgac aatcaccacg
     6961 ctggcaatac cgttggcacg caaaaccgga cggatgttgt tcaggttttc ataagtgttg
     7021 cgcgaagtgt tttcaaacag gatgttgcgc gccggaaccc cctgtttgag cgcgtaccgc
     7081 cgcccgacct cggcttcggt catatagcct tttttggttc gccctcccgt aaacacgatt
     7141 ttgcctaccc tgcggctttg gtaaagcgcg atggcgtggt tgatgcgttc ccgaaaaaca
     7201 ggggaagggc gtttgtccca tgcggcggca cccaacacca gcgcggcatc cgcccggaca
     7261 tacggcggca aaacctgccc gcccgtccga taaaccgccc aaacggatga ggtaaacacc
     7321 agcaaaagcg gaaaaacact caaacagaaa ccgcccaaca ggtaatagcg caagccgttg
     7381 cggctgcaaa acagccgttt gttcacaata ccgcttcgat attttccaac ggcctgccga
     7441 cagccgcctt accgtttgcc aaaacaatcg gacgctccaa cagggcggga tgatcggcga
     7501 tggcacgcag cagtgcgtta ttgtccaaat cggggttgtc caaacccaat tccttgtaca
     7561 aatcatcttt cacacgcatc atcccgcgcg ccgactccaa gcccaatttg ttgaaaatat
     7621 ccttcaattc ggacaagtcg ggcggcgtat ccaaatattt gaccacttcg gcagcaatgc
     7681 cgcgttcttc caatagggac aaggcggcac gcgatttgct gcaacgcgga ttgtggaaaa
     7741 ttttgatttc agacatgaca tttccttact tctcgacaat ccccttatta tcggcttaca
     7801 cagggtttta ctcaatatcc cgcctacaac cgtaccaaac ggtttacaat acccgaatcg
     7861 acatacaaag gacaaaacga tgaaatactt gaatcttgcc gcaatcaccc ttggcgccac
     7921 atttgccgca cataccgcct cggcagacga actggccgga tggaaagacg gtgccgcgca
     7981 aagcctgcaa tcgctcaaag cccccgtccg catcgtcaac ctttgggcga cctggtgcgg
     8041 tccgtgccga aaagagatgc ctgccatgtc caaatggtac aaagcgcaga aaaaaggcag
     8101 cgtcgatatg gtcggcatcg cgctcgacac atccgacaat atcggcaact tcctcaagca
     8161 aacccctgtt tcttacccga tttggcgtta cactggggcg aacagccgaa actttatgaa
     8221 aacctacgga aacactgtcg gcgtactgcc ctttaccgtc gtcgaagcac cgaaatgcgg
     8281 atacaggcag accattaccg gggaagtgaa cgaaaaaagc ctgaccgaag ccgtcaaact
     8341 cgcccattca aaatgccgtt aaacgccgga tgccgtctga atccgcttca gacggcattt
     8401 ttcccgcccg accttcggta tccgccaaac ttatccacta tctaaaaaca ggcggaatct
     8461 ttataatcgg cactgtctta cctattgttc agacggcata tccctgcgga cgcaaccgcc
     8521 cgaaacgata tgccgcccat tccttacagg acctcctatg atccgtttcg aacaagtttc
     8581 caaaacctat cccggcggtt ttgaagccct gaaaaacgtc agcttccaaa tcaacaaagg
     8641 cgaaatgata tttatcgcgg gacactccgg ttcgggcaaa tccaccgtcc tcaagctgat
     8701 ttcgggcatt accaaaccaa gcaggggcaa aatcctgttt aacgggcagg acctcggcac
     8761 attgtccgac aaccaaatcg gctttatgcg ccaacacatc ggcatcgtgt tccaagacca
     8821 caaaatcctc tacgaccgca acgtcctgca aaacgtcatc ctgccgcttc ggattatcgg
     8881 ctatccgccg cgcaaagccg aagagcgtgc ccgcatcgcc atcgaaaaag tcggcctgaa
     8941 aggacgagaa ttggacgatc ccgtaaccct ctccggcggt gaacaacaac gcctgtgcat
     9001 cgcccgcgcc gtcgttcacc agcccggcct gctgattgcc gacgaaccct ccgccaacct
     9061 cgaccgcgcc tacgcgctcg atattatgga attgttcaaa accttccacg aagcgggaac
     9121 aaccgtcatc gtcgccgcac atgacgaaac cctgatggcg gactacggac accgcatcct
     9181 gcgcctctcg aaaggacgac tcgcatgagc atcatccact acctctcgct gcacgtcgaa
     9241 tccgcgcgca ccgcgctcaa acagcttctg cgccaacctt tcggcacact gcttaccctc
     9301 ataatgctcg ccgtcgccat gaccctgccg ctgtttatgt atttgggcat ccaaagcggg
     9361 caaagcgtgt tgggcaaact caacgagtcg ccgcaaatca caatctatat ggaaaccgcc
     9421 gccgcacaaa gcgacagcga taccgtgcgc agcctgctgg cgcgcgacaa acggctcgac
     9481 aacatccgct tcatcggcaa agaagacggt ctggaagaat tacagtccaa tctcgaccaa
     9541 aatctgattt ccatgcttga cggcaacccc ctgccggatg tctttatcgt tacccccgac
     9601 ccggcaacca cgcccgccca aatgcaggca atctaccgag acattaccaa actgcctatg
     9661 gtcgaatccg cgtctatgga taccgaatgg gtgcaaacgc tgtaccaaat caacgagttc
     9721 atccgcaaaa ttttatggtt tctttccctg acgctgggga tggcgttcgt ccttgtcgcg
     9781 cacaacacca tccgcctaca aatcctcagc cgaaaagaag aaatcgaaat caccaaactc
     9841 ttgggcgcgc ccgcgtcgtt tatccgccgc cccttccttt atcaggcaat gtggcagagc
     9901 atcctttccg ccgccgtcag cttggggctt tgcggttggc tgctctctgc cgtgcgccca
     9961 ttggtcgatg ccattttcaa accctacggc ttgaacatcg gctggcgttt cttttaccct
    10021 gaagaaatcg tgctggtgtt cggcttcgtc atcgcgttgg gcgtattcgg cgcgtggctt
    10081 gccaccaccc agcacctgct cggcttcaaa gccaaaaaat aaaacaccgt caaaaatgcc
    10141 gtctgaaccc gtttcagacg gcatttcaat ttgccagtat aatggcgcat ttttccaaca
    10201 aggaacctac catgctgacc ccggaacaag taaaagccat gattgaagac gtggcaaaat
    10261 gcgaacatat caaagtagaa ggcgacggac accatttttt cgccgtcatc gtttcatcag
    10321 aatttgaagg caaggcacgc ctcacgcgcc accgcctgat taaagacgga ctcaaagccc
    10381 aactggaaag taacgaactg cacgcacttt ccatttcggt tgccgccact ccggcggaat
    10441 gggcagccaa agcacaataa tcgccacaca aaaatgccgt cagaaacagc acaacgtttc
    10501 agacggcatt ttttatatca aaccgcttac gcgccgcgtt tttccaaagc ggctacggca
    10561 ggcaactctt taccttccaa gaac
//
LOCUS       DAWHXZ010000052        10515 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000052 DAWHXZ010000000
VERSION     DAWHXZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10515
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.052"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(187..2595)
                     /locus_tag="WIE35_000850"
     CDS             complement(187..2595)
                     /locus_tag="WIE35_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ4153787.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEQGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPPLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLYATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            2665..3669
                     /locus_tag="WIE35_000851"
     CDS             2665..3669
                     /locus_tag="WIE35_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ4153788.1"
                     /translation="MQRQIKLKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(3726..4424)
                     /locus_tag="WIE35_000852"
     CDS             complement(3726..4424)
                     /locus_tag="WIE35_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ4153789.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMHTID
                     LLADKVREEQKAAQ"
     gene            4642..6180
                     /gene="murJ"
                     /locus_tag="WIE35_000853"
     CDS             4642..6180
                     /gene="murJ"
                     /locus_tag="WIE35_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ4153790.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFGIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LSFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFDWAHAGGMQKAARLFILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            6313..7269
                     /locus_tag="WIE35_000854"
     CDS             6313..7269
                     /locus_tag="WIE35_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ4153791.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRSRGWHGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGNRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHVGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            7320..8102
                     /gene="trpC"
                     /locus_tag="WIE35_000855"
     CDS             7320..8102
                     /gene="trpC"
                     /locus_tag="WIE35_000855"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ4153792.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVPLEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEREQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIEAEVGKLF"
     gene            8167..10467
                     /gene="recQ"
                     /locus_tag="WIE35_000856"
     CDS             8167..10467
                     /gene="recQ"
                     /locus_tag="WIE35_000856"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ4153793.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMCEGVAVVVSPLIALMNDQVANLHAAGIEAAAVNSGTSADGAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGI
                     LAERYPNVPRIALTATADAATRADIKHYLHLDNAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEESEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEVIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKIDRFGHGILEVCRNTAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKRKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPKGLAELA
                     AVYGVGEEKTARYGAAVLAVLGQNAV"
BASE COUNT         2134 a         2985 c         3032 g         2364 t
ORIGIN      
        1 cggtttttgc cttttgcggt gcggcgtgga catcggctgc tgccagcaat gcggcggcaa
       61 tcatcagggc tttgattttc atcattcttt ccttatcgtt tgaatcctgc ctttgatggc
      121 gggtttgggg ttgtcggtgg aatgccgtct tgtgcgggaa cggtgctcct gccgggtttc
      181 cgacggtcag ggccgtttgt tgcgtccggc ggaaagagag tgggcgggga tgtagccggg
      241 aacggcgaca tccatcctgt ctgcggggtt tctgccgaca ctgctgaggt ctttcaactg
      301 aagtgagaaa aagacagcgt ttttgtaggt gttttcgccg gtaacgtagc gttgggcgta
      361 cacgcccgcg ccccagcagc cgcaactgct tttgtattcc gcacccgcca gcacctctat
      421 cggttttttg gcttcaaaac cgtagttgta acggacgacg gccgacaggt tgcgcgtcag
      481 cggccattgt gcggacaggt cgagctggct gagtttgtcg taaaaatagg aaccgtcgga
      541 cttcaggtag attttttcgt tgcgcccgta tttgtagcgg gcgttcagca ctttgccctg
      601 tgcgggacgg tagcttgcac cgacggcgta gttctcggcg cgtttgtcgt tttggttgta
      661 gtggatgctg ctgtcgagga tgaagcggct gccgatgctg ccggaggcaa atgccaccca
      721 gtcggaacgg ttgcgcggtt ttttgccgac gctgccgtca agcatcaccg catcatcctt
      781 gaaatagaat ttctgaccga tgccggcgcg gaaacgctct tcccccgtcg cgccgtccaa
      841 aatacggctt tgcacggcgg cggaaaggct gtttgcggtg ttaatcctgt cgttgccgta
      901 atagaggttt tcgcgaaaga gctgcccgta gccgaagctg ctttccgacg aatcgaaatt
      961 gggcaggtcg ttttgggatt tggcaggaat atagttgtag aacaggcgcg gctcgagggt
     1021 ttgcaggact ccgccgccga acatccgcgt attccgctca aaagttgcgc cgctgtcgat
     1081 gttgacaatg ggcagagtgc ggctgacgcg tcgggcttct tggctgccga agcggttgag
     1141 gctgtaatag gtggcgtaca gtccgagttt gggacggacg taaccccagc tgttgctgaa
     1201 atcccatttg atgtcgggat agacgacgag gcggctgccg tcttggcggc tgtcgtggct
     1261 gaagcgggta aattgtgcgg acacgccgat ttgcgccctg ccggtgtttt tgcgccaatc
     1321 ggcggaaagg cgcggcatca gggcatacgg tttgtctttg tagccgcttt ggtttgccag
     1381 cgtctggtat ttcagaaccg aaaggccggc attcaggctg ccgcccgccg ccctgccgcc
     1441 ataatccagc catacacggc ggttgaggtt gacgttgccg gcgatttctt tgttgccgta
     1501 aaagtcgcgg tagtagccgc tgtcggagac ttggttgaaa tcgacacccg cctgaagcgt
     1561 gtcggaaatg tcgtgccggt gctgccattt cgcctgatag cggttattcc tgccgctttt
     1621 tttgtcgtgc ggcagccagg tcaggtcgga ctggccggca taatccggcc gcaggtagcg
     1681 tacctgcccg tcaaagaccg cgccgcgttc gccgatcacg ccgggcgcga acgtggcatc
     1741 gagattgggg gcaaggttga aataataggg aacggaaagg gaaacgccgt ccgaaccggc
     1801 ggacagtggg ggaacaagca ggccgctttt gcggttgccg tcaagcggga agtccgccca
     1861 aggcgtatag aaaaggggaa cgccgccgaa cacgaaggcg gcgtgtttgg caacgcctat
     1921 gcctttttcc cgatcggctt cgacggaagc ggccttgaca taccagccgg catcgccggc
     1981 ggaacaggtg ttgaattggg tttccgtcag tttgtaatgc ccttcgccca acatttcggc
     2041 ggtgcggctg acgctttgca gccgccgtcc gccttgttcg gtttccatac ggacgttgtg
     2101 cgcttccccg gtctgctgct cgagattgta ggtcagggtt tcgccccgaa tcagcgtacc
     2161 gtcctgttgg agggcgaacc ggtcgcctgc ggtaacggtg tcgcccgact ggtcgtaatc
     2221 cgcccaatcg gtattgaggg tcgtccggtt gcgttcgacg acgacgttgc cttcggcacg
     2281 cacctgcacc tgcgactgtc cttccacctt gtcggcaaca atgcgcgtat agtcttcggg
     2341 gatggatgct tcgccgctgc ctcggacggc ggcttcggtt ctctcggggc tgccgctttc
     2401 gttactgcaa aacaggcagg tcgaaccgag gctcaggctg gtaggctgta tgggttcgga
     2461 cacgctccga acgcttcctc cggcggtcgg attgtccgtt tcctccgccg caacggcatc
     2521 ggcggcggcg caatgcgtgc cgaagcagag gcccaatgcc agcaccagtg gtttgagtga
     2581 aaataaacga gccaaaatcg cccctcaagt cggtttgcca gttagaatag cgtttattgt
     2641 aacctgaaat gctttagtac tgttatgcaa cgacaaatca aactgaaaaa ttggcttcag
     2701 accgtttatc ccgaacggga cttcgatctg acttttgcgg cggcggatgc tgatttccgc
     2761 cgctatttcc gtgcaacgtt ttcagacggc ggcagcgtcg tctgtatgga tgcgccgccc
     2821 gacaagatga gtgtcgcgcc ttatttgaag gtgcagaaac tgtttgatat ggtcaatgtg
     2881 ccgcaggtat tgcacgcgga cacggatttg gggtttgtgg tattgaacga cttgggcaat
     2941 acgacgtttt tgaccgcgat gcttcaggaa cagggcgaaa cggcgcacaa agccctgctt
     3001 ttggaggcca tcggcgagtt ggtcgaattg cagaaggcaa gccgtgaagg ggttttgccc
     3061 gaatatgacc gtgaaacgat gctgcgcgaa atcaacctgt tcccggaatg gtttgtcgca
     3121 aaagaattgg ggcgcgaatt aacattcaaa caacgccaac tttggcagca aaccgtcgat
     3181 acgctgctgc cgcccctgtt ggcgcagccc aaagtctatg tgcaccgcga ctttatcgtc
     3241 cgcaacctga tgctgacgcg cggcaggccg ggcgttttag acttccaaga cgcgctttac
     3301 ggcccgattt cctacgattt ggtgtcgctg ttgcgcgatg cctttatcga atgggaagaa
     3361 gaatttgtct tggacttggt tatccgctac tgggaaaagg cgcgggctgc cggcttgccc
     3421 gtccccgaag cgtttgacga gttttaccgc tggttcgaat ggatgggcgt gcagcggcac
     3481 ttgaaggttg caggcatctt cgcacgcctg tactaccgcg acggcaaaga caaataccgt
     3541 ccggaaatcc cgcgtttctt aaactatctg cgccgcgtat cgcgccgtta tgccgaactc
     3601 gccccgctct acgcgctctt ggtcgaactg gtcggcgatg aagaactgga aacgggcttt
     3661 acgttttaaa cccaatcaaa atgccgtctg aaaaccaagt ttcagacggc atttttcaaa
     3721 cgggcttact gcgcggcttt ttgttcttcg cgtactttgt ccgccaaaag gtcgatggtg
     3781 tgcataccgg actcccagtc ggcaaattcg actttatatt tgccgccgac gataaccgtg
     3841 ggcgtaccgt cgatttggaa ggtttcggtc agctcctgca ttttgtcggc gcgcgcctgg
     3901 ctttcggggg actcgtaggc ggcaaggact tttttgccgt caaaggcggt ttgttcgccc
     3961 agccattttt tgaggacttc cggctcttgc agcttgattt tttggttgac catcgcatcg
     4021 aaaatatggc tgttcgccac atctttgctg tcggcggcag ccatatcgac ggcggcggcg
     4081 aggcgtgcga gcgtcagcat ttctttctgc cagacgacgt gttcggtacg caggtacata
     4141 tcgtctttaa aagacttggc gtgtttgctt aaaacaggtt cgaggtgggc gcagtgcgga
     4201 cagaaatagc cgaaaaattc aaggacttcg actttgcctg cctgctgttg gggaatcggg
     4261 ttggcaagga cggtatagtt ttgcccttcg accagccctg ccggggcggc ggctgccgaa
     4321 gcggcaggcg cgctgtcggc ggggacgctg gtttggactt tgctgtcgca cgcggcaagg
     4381 gcgaacaggg cggcaacgcc gagggcgagg tgtctggatt tcatacggct ctccgtgatg
     4441 ttggaaacag atgttggaaa tcaatcggaa tccggctatt ttattgcatt tccgcgtgtt
     4501 gatacagttt gccgccggaa aaggaagctt tcgtttcggg aaaccgcttc agacggcatc
     4561 aaacccgatg ccgtctgaag cggtttctgt cgtacaatac gcgccgttgc cccaaacggg
     4621 tacgactgtt gaggaacaat gatgaatatg ctgggagctt tggcaaaagt cggcagcctg
     4681 acgatggtgt cgcgcgtttt gggatttgtg cgcgatacgg tcattgcgcg cgcattcggc
     4741 gcgggcatgg cgacggatgc gttttttgtc gcgttcaaac tgcccaacct gcttcgccgc
     4801 gtgtttgcgg agggggcgtt tgcccaagcg tttgtgccga ttttggcgga atataaggaa
     4861 acgcgttcta aagaggcgac ggaggctttt atccgccatg tggcggggat gctgtcgttt
     4921 gtgctggtca tcgttaccgc gctgggcata cttgctgcgc cttgggtgat ttatgtttcc
     4981 gcacccggtt ttgccaaaga tgccgacaaa tttcagctct ccatcgattt gctgcggatt
     5041 acgtttcctt atatcttatt gatttcactt tcctcttttg tcggctcggt actcaattct
     5101 tatcataagt tcggcattcc ggcgtttacg cccacgttcc tgaacgtgtc gtttatcgta
     5161 ttcgcgctgt ttttcgtgcc gtatttcgat cctcccgtta ccgcgctggc ttgggcggtc
     5221 tttgtcggcg gcattttgca gctcggcttc caactgccct ggctggcgaa actgggtttt
     5281 ttgaaactgc ccaaactgag tttcaaagat gcggcggtca accgcgtgat gaaacagatg
     5341 gcgcctgcga ttttgggcgt gagcgtggcg cagatttctt tggtgatcaa cacgattttc
     5401 gcgtcttatc tgcaatcggg cagcgtttca tggatgtatt acgccgaccg catgatggaa
     5461 ctgcccggcg gcgtgctggg ggcggcactc ggtacgattt tgctgccgac tttgtccaaa
     5521 cactcggcaa accaagatac ggaacagttt tccgccctgc tcgactgggg tttgcgcctg
     5581 tgtatgctgc tgacgctgcc ggcggcggtc ggactggcgg tgttgtcgtt cccgctggtg
     5641 gcgacgctgt ttatgtaccg cgaattcacg ctgtttgacg cgcagatgac gcaacacgcg
     5701 ctgattgcct attctttcgg tttaatcggt ttgatcatga ttaaagtgtt ggcacccggc
     5761 ttctatgcgc ggcaaaacat caaaacgccc gtcaaaatcg ccatcttcac gctcatctgc
     5821 acgcagttga tgaaccttgc ctttatcggc ccactgaaac acgtcggact ttcgcttgcc
     5881 atcggtctgg gcgcgtgtat caatgccgga ttgttgtttt acctgttgcg cagacacggt
     5941 atttaccaac ctggcaaggg ttgggcggcg ttcttagcaa aaatgctgct ctcgctcgcc
     6001 gtgatgggag gcggcctgta tgccgcccaa atctggctgc cgttcgactg ggcacacgcc
     6061 ggtggaatgc aaaaggccgc ccggctcttc atcctgattg ccgtcggcgg cggactgtat
     6121 ttcgcatctc tggcggcttt gggcttccgt ccgcgccatt tcaaacgggt ggaaagctga
     6181 ccgatgccga tatccttttt cggaaaacgc tttttccgcg cctatattcg ggcgcgcatc
     6241 ccccgcgtcc gcaccagccg gacgggctgc cgcatcatca atgccgtctg aaaacagaaa
     6301 aaccgatacc ttatgatttt gacaccgccg gacacaccgt ttttcctccg caacggcaat
     6361 gccgatacga ttgccgccaa attcctgcaa cgccccgcac ccgcataccg ccgcgagctg
     6421 cttcccgaca gcacgggtaa aaccaaagtc gcctacgact tttcagacgg catttcgccc
     6481 gatgcgccgc tggtcgtgct gtttcacggt ttagaaggaa gcagccgcag ccattacgcg
     6541 gtcgaactga tgctcgcggt acgcagccgc ggttggcacg gcgcagtcgt ccatttccgc
     6601 agctgcggcg gcattgccaa caccgctccg gtgttctacc acttgggcga taccgccgaa
     6661 atcgccttta ctttggacac gcttgccgcg cgttaccgtg aaatatacgc cgtcggcgta
     6721 tcgctgggcg gcaacgcgct ggcaaaatat ttgggcgaac agggcgaaaa cgcgctgccg
     6781 caagccgccg ccgtcatctc cgcacccgtc gatgcagagg cggcaggcaa ccgcttcgac
     6841 agcggcatca cacggctgct ctacacgcgc tacttcctcc gcacactgat acccaaagca
     6901 cggtcgctcc aaggttttca gacggcattt gccgcagggt gcaaaacact gggcgagttt
     6961 gacgaccgct tcaccgcacc gctgcacggc tttgccgacc ggcacgacta ctaccgccaa
     7021 acttcctgca aaccgctgct caaacacgtt gccaaaccgc tgctcctgct caatgccgcc
     7081 aacgacccct tcctgccgcc cgaagcgctg ccgacaccgg acgaagtgtc cgaagccgtt
     7141 accctgttcc agccggcaca cggtggtcat gtcggctttg tcagcagtac cggcggcagg
     7201 ctgcacctgc aatggctgcc gcagaccgtc ctgtcctatt tcgacagctt ccgcacaaac
     7261 aggcgttaac ggtttgatgc taatattccc cctttccccg gacgaatacg gaacacaata
     7321 tgaccgacat cctcaataaa atccttaccg ccaaggcaca ggaagttgcc gcccaaaaag
     7381 ccgccgttcc cctcgaacac atccgcgcac ttgccgcaga agccgcgccc gtccgaagct
     7441 tcatcggttc gatacgcggc aaacaccgcc taaacctgcc cgccgtcatt gccgaaatca
     7501 aaaaggcaag cccgagcaaa ggcctgatcc gtccggactt ccgaccggcc gagattgcac
     7561 gcgcctatga aaacgccgga gcggcgtgtt tgtccgtact gaccgacgaa ccctatttcc
     7621 aaggttcgcc cgaatacctc aaacaggcgc gcgaagccgt atcgctgccc gtcttgcgta
     7681 aagacttcat catcgacgaa tatcaggttt atcaggcgcg cgcctggggg gcggatgccg
     7741 tcctgctgat tgccgccgca ctggaacggg aacaattgga acgctttgaa gcggtggcgc
     7801 acgaattggg tatgaccgtc ctgctcgagc tgcacgatga aagcgaattg gaaaaatgcc
     7861 gtaacctgac cacgccgttg tggggcgtaa acaaccgcaa cctgaggact tttgaagtct
     7921 ccctcgacca aaccctgtcg ctgctgcccg cactggaagg caaaaccgtc gttaccgaaa
     7981 gcggcattac gggtaaggcg gatgtggaat ttatgcgggc gcgcggcgtg cacaccttcc
     8041 tgatcggcga aacgttcatg cgtgccgacg atattgaggc ggaagtgggc aaactcttct
     8101 aaatcccgat ttcagacggc atattgccgc caaccgacca acacccttac cgagcacgga
     8161 accatcatgc accgaccgac cgccaaacag attctgcacg aagtatttgg ttatcccgaa
     8221 tttcgcggca ggcaggaggc tgtcatcaat actttggcag gcggcgggag tttgacggtg
     8281 ctgatgccga cgggcggcgg caagtctttg tgttaccaga ttcccgcgct gatgtgcgaa
     8341 ggcgtggcgg ttgtcgtatc gccgctgatt gcgttgatga acgaccaagt ggcaaacctg
     8401 cacgccgccg gcatcgaagc ggcggcagtc aacagcggca catcggcaga tggggcgcgc
     8461 gagattgccg acaagcttgc ccaaggccgt ctgaaactgc tttatgtcgc accggaacgc
     8521 ctagttaccg accgcttttt gcgttttctc gaccaacaaa ccgtcagcct gttcgccatt
     8581 gacgaagcgc actgcgtcag ccagtgggga cacgatttcc gccccgaata ccaacagctc
     8641 ggcatccttg ccgaacgcta tccgaacgtc ccgcgcatcg ccctgaccgc taccgccgat
     8701 gccgccacgc gcgccgacat caagcattat ctgcacttgg acaatgcgcc cgaatttgtc
     8761 tccagcttcg accgtccgaa tatttattat caggttatcg aaaaaaacaa cggcaaaaaa
     8821 caattgctgg atttcatccg caaagaaatg acggggcaaa gcggcattgt gtattgccta
     8881 agccgcaaaa aggttgaaga tgtggcgcag tttttgcgtg aaaacggatt aaacgcgatt
     8941 ccgtatcatg ctggtttgag catggacgtg cgcgaggaaa accaacgccg ctttacgcat
     9001 gaagacaata ttatcgtggt ggcgaccgtg gcgttcggca tgggcataga caaacccgac
     9061 gtgcgctttg tcgcccatct cgatatgccc cagagtgtcg aacatttcta tcaggaatcg
     9121 gggcgtgccg gccgggacgg gctgcctgcc gcgagctggc tgtgttacgg tttgaacgat
     9181 tgggtgttgc tgcgcgaacg gattgccgaa ggcaacagcg acgaggtgca aaagcaaatc
     9241 gaaatgcaaa aactcgatgc catgctttcc gtctgcgaaa ccgccgcctg ccgccgcgta
     9301 ctgctgctca aacatttcgg cgaagaatcc gaaccctgcg gccattgcga caactgcctg
     9361 catccgcccg tacggtttga cggcacggtg ttggtgcaaa aattactcag ctgcgtgtac
     9421 cgcgccggac aacgttttgc cgccggttac atcaccaacc ttttgcgcgg caaaagcgac
     9481 gattggatac gcggcaaccg gcacgaacaa ctgtccacct tcggcatcgg tacggagttg
     9541 tccgacaaag aatggcgcag cgtcatccgc cagtgtatca gcctcggcta cctcaccgtc
     9601 aacattaccc gatatcaggc attgcaactg accgaagccg ccaaaaaagt cctcaaaggc
     9661 gaaaccgaag tgatgctgcg tccgctcaag cgcgacaagc ccgccgcccg caccctcaaa
     9721 gacaactggc tgcgtaccga acgcgaagaa cgcctgtggc aggcattgcg cgtttggcgt
     9781 atgaaacagg cagaagccga agtcatcccc gcctatatga ttttcggcga caaaaccctg
     9841 cgcgaccttg tcgaaaaaat gccgcaagac ctcaacggac tgcacgacat ctacggtttg
     9901 ggcgaagcca aaatcgaccg tttcggacac ggcatcctcg aagtctgccg caacaccgcc
     9961 ggcttcagtc gcgacgcggt catccgtccg caaaccgaac gcgaacaaca actgcgtcaa
    10021 aaactcgagg cctggcggta tgaacaggca agggcggaaa actgcgccct gcatgccgtc
    10081 ctctccgacg aaagccttgc cgatatgctt gccgccacgc ccgaaaccga aaccgacctc
    10141 gaaggcgtac acggcttggg cagcgtacgc gccgccaaat acggacggga catcctcgcc
    10201 gtctgccgtc cgttttcaga cggcatcgat gaagccgcca aacgcaaacg ccgcctgatg
    10261 cgcgccctga tccaatggtg cggcgaaacg gcaaaacacg aacagtccga accctaccgc
    10321 attctcagca aagccgccct gcgcgccatt gccgccaaac agccgaaggg tttggcggag
    10381 cttgccgccg tatacggcgt gggcgaagaa aaaaccgcac gttacggtgc ggcggtgttg
    10441 gcggtgttgg gacaaaatgc cgtctgaaaa ccgttaccaa gtttcagacg gcattgcctc
    10501 tatttaaaaa ttcct
//
LOCUS       DAWHXZ010000053        10502 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000053 DAWHXZ010000000
VERSION     DAWHXZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10502
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.053"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(72..839)
                     /locus_tag="WIE35_000857"
     CDS             complement(72..839)
                     /locus_tag="WIE35_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ4153794.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(927..1754)
                     /gene="mutM"
                     /locus_tag="WIE35_000858"
     CDS             complement(927..1754)
                     /gene="mutM"
                     /locus_tag="WIE35_000858"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ4153795.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPYRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1806..2471)
                     /locus_tag="WIE35_000859"
     CDS             complement(1806..2471)
                     /locus_tag="WIE35_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ4153796.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGRWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRRLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYALVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            2650..4626
                     /locus_tag="WIE35_000860"
     CDS             2650..4626
                     /locus_tag="WIE35_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ4153797.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASRSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4694..5386
                     /locus_tag="WIE35_000861"
     CDS             4694..5386
                     /locus_tag="WIE35_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ4153798.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYILLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLESPSALLLIITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIDCPKL"
     gene            complement(5587..5693)
                     /locus_tag="WIE35_000862"
                     /pseudo
     CDS             complement(5587..5693)
                     /locus_tag="WIE35_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            6019..6315
                     /locus_tag="WIE35_000863"
                     /pseudo
     CDS             6019..6315
                     /locus_tag="WIE35_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            6285..7331
                     /locus_tag="WIE35_000864"
     CDS             6285..7331
                     /locus_tag="WIE35_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ4153799.1"
                     /translation="MAYYMVLGGWVISYIVNIIGGNLDISSPVSSEVTKSFFTEHIEN
                     SPWEIAFYTFLFVVVNQWILVKGVIGGIEKAAKYLMPLLFLFLIAMVVRNVTLPGAME
                     GITFYLKPDFSKITAELFVFVLGQVFFTLSLGFGVMITLSSYLDKNENLVQTAVITAI
                     TNTIIAVLAGFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSHMWAGPVFAVIFFSLLLI
                     AALTTSLTIYEVLITTIQEKTKIRRTAAITIVLSAIFIFGNIPSILSYGPWKDVSIFG
                     KNIFDAFDYISGNILFMLTALGSALFVGFVMKDEAKDELLYKGNHITVNIWFAYVKYL
                     VPLVILLIFVSNLF"
     gene            7575..8231
                     /locus_tag="WIE35_000865"
     CDS             7575..8231
                     /locus_tag="WIE35_000865"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ4153800.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSKLAKKLPAVFSGSRILLDGEDVSDGIRTEAIGMGASAVAQLP
                     KVRASLLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            8386..10071
                     /gene="rpsA"
                     /locus_tag="WIE35_000866"
     CDS             8386..10071
                     /gene="rpsA"
                     /locus_tag="WIE35_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ4153801.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVVALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            10082..10396
                     /locus_tag="WIE35_000867"
     CDS             10082..10396
                     /locus_tag="WIE35_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ4153802.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
BASE COUNT         2745 a         3084 c         2362 g         2311 t
ORIGIN      
        1 gtactggttt aaatttaatc cactatacat ccacaaaagc gaatcatact gccacattcc
       61 gacaaccggt ttcagacagc gatatccgca tcgtcggcga caacggcacg gatgctttct
      121 tcgattttat cttttaaagc ataacggtct tcgctttccg ccgcatccgc cacgcaaacg
      181 aaatcgactt ttatcgtcaa ttttttcata gacacgatgc gccacaggca ggtcggcaaa
      241 ccgacatcgg catatgaggg acgagccgtc ctttttcccg tttcgtcata ataacgcagc
      301 gcgaccgcca aaacctttgc ccccgcatcg atggcggatt ggaacagcgc ggctttgaac
      361 ggcaaaagcc ccaatccgga ggaagtccgc gcttcgggga aaaaactgac gttttgaccg
      421 cgttgcaagg tttcgcagac ggcgcggtta atcggttcga tgtcgcgccg cgaattgcgg
      481 ttgatgaaca ccgttcccgc gttctgcccc atcttgccca ataccggcca gcttttgatt
      541 tcctgcttgg cgataaagct gctcggataa accgcgctca tcgcgaaaat atccagccag
      601 gacacgtggt tggcggcaac caagacaccg ttcggatgtt cgggtgcggg tctgcccacc
      661 tccaatccga tatccaaagc cgccaaaacc cccctgccca actcgattac cgcccgattg
      721 cgcgactcgg ggcaaccgcc gtcaataccg cgcaggtttt tcccggtttt gaacagccag
      781 accgccaaac ggcacaagcg gcgcagacgt gtaaaaaatg aagctttatt tgaagacatg
      841 agcaattctt tcccgataat cggtgtttgt tgttgaaacg ttcggacggc ataccgataa
      901 aatgccgtct gaaaccgttt tcagtcctat ttctgacagt tcgggcaata aaacgtgccg
      961 cgctgcccca aagtttcttt cacaaccaaa ccgccgcact gggggcacgg ctgattgtgc
     1021 cgcccgtaca ccgtatattc ctgttggaag tagccgcttt tgccgtcgct gtccacaaaa
     1081 tccctcaagg tactgccgcc cgtttcaatg gcgcgccgca acaccgcttt gacggtttca
     1141 accaaaagcg cgcactcttt ctttttcagg cggttggcag gacggtaggg cgaaatgccc
     1201 gccctaaaca ggctctcgtt ggcataaatg ttgcccacac cgaccacgac cgcattgtcc
     1261 atcagggcaa gtttgaccgc gcgcttctgc gccttcagcc ttgcatacag ataatccgca
     1321 caaaatgcct ccgacaaagg ctccggcccc agtttttcca acagcggatg atgttcttcg
     1381 attccctcat accaaagtat cgcgccgaac tttctcggat cgcggtaacg catgaccgtg
     1441 ccgtctgaaa acacaatatc gacgtgatcg tgtctgtccg gcctgccgat acgtccgtcc
     1501 gacggcgtaa aaatccgcaa gctgcccgac atccccaagt gaatcagcag cacgcccgtt
     1561 tgaaagcgga taagcaggta tttcgccctc ctgccgcagg acaacacctg ccggccggac
     1621 aaaatctccc ccaaatcggg attaatctgc cagcgcagct tcaattggcg caataccacg
     1681 gcttccaccg ttttcccttc aatatgcggc gcgatgccgc gcaacgtcgt ttccacttcc
     1741 ggcaattcag gcataacccc tcccgacatt ttttctgaca gatgccgtct gaaagacggc
     1801 cgtccctaat ccgcaaccct tgccgcaccc gccgcaaggg ctttgccgcc caaatacccg
     1861 tcccacgcgg gcaggggcgt tacgcccgcc gcccttttga ccaaaaccaa agcgtacact
     1921 gcggcacatt gcggccacca acggtcgccc gccttttcca taaaccgcca aaagcgtatt
     1981 tgcccgagcg acgaaaccgg cggcagatac accataaatt tcccaaattc aatatcgaaa
     2041 ccggcatcgg caagccgcct tttcaactcg ggcagcggca gacaaaaccg tttttccggc
     2101 aggcgttcgc cgtcaaacca acggctgaat ccccagagcg aatacggatt gaaacccgtc
     2161 agcatcaagc gtccgcacgg tttcaatatc cggtgcgctt ccgacaggat ttgcgaagga
     2221 acaccgcctt caagcgtatg cggaaaaagc agcatatccg cagaaacatc cgccaaagcc
     2281 atattctccg ccgacatcga catatctcgc ggcacacaga caacatcttc agacaggctc
     2341 agccacctgc cgcccacctg aaccgcacac attcccgaaa aacggtatga atccagatac
     2401 cgcccgaaga aatcctgttc caattttgca acataccgcc ccatcgccgt atcttcaaac
     2461 catgcatcca tattgcgtcc gtttcaaaca gattgcctgc cgatattcta ttccaaacag
     2521 gatttctgtc aaaaaacaca tcggccgccc atttccgaat ccgcataaag ttcctgtaaa
     2581 acttgacgct ttttcagtca aacagtacca tcggacgata aaatatgttt attccgccga
     2641 atataaatca tgtccaaact caaaaccatc gctctgaccg catcaggtct gtccgtttgt
     2701 ccgggtttcc tatacgccca aaacacctca tcacaccaaa tcggtttggc gattatgcgc
     2761 ttaaactctt caatactcga cctgcccccg acaaaacaat atttccaatc cggcagcctg
     2821 tggagcgagc tgcgccaagg cttccggatg ggcgaagtca atcccgaact ggtacgccgc
     2881 cacgaaagca aattcatcgc aagccgcagc tatttcaaca gggtcatcaa ccggagtaga
     2941 ccctatatgt accatatcgc caacgaagtc aaaaaacgca atatgcccgc cgaagccgcc
     3001 ctgcttccct tcatcgaaag cgcgttcgtc accaaagcca aatcacacgt cggcgcatca
     3061 ggattatggc agtttatgcc cgctaccggc aggcattacg gcctggaaaa aacaccggtt
     3121 tacgacggca ggcacgacgt ttacgccgcc accgatgccg cactcaacta tctgcaatac
     3181 ctctatggac tgttcggcga ctggccgctt gcctttgccg cctacaactg gggtgaaggc
     3241 aacgtcggac gcgccatcaa ccgcgcccgc gcccaagggc tcgaaccgac ctacgaaaac
     3301 ctgcgtatgc ccaacgaaac gcgcaactat gtccccaagc tgctcgccgt gcgcaacatt
     3361 attgccactc cccaatcttt cggcatgaat atcagcgaca tagacaacaa accctatttt
     3421 caggcagtcg aaccggatcg tccgctcgac aacgaagcca tcgcccggct tgccggcatc
     3481 acgcaaagcg agctgctcgc cctaaacccc gcattcaacg tccccgcgtt tatccccaaa
     3541 agcaaacgca aactgctgct tcctgtcgcg tccgtacaaa ccttccaaag caactacctc
     3601 aacgccgcac ccgacagcct gttttcatgg gaagtctata cgcctgccgc caaaaccagc
     3661 ctgtccgaca tctcgacggc aaccggcatg agcattgccg acatcaaacg cctcaacaac
     3721 ctgaacggca accttgtcaa cgcaggacgc agcatccttg tcgccaagaa cggcaaaacc
     3781 cttcagacgg catcggaatc cgtcgtttcc atcgacatcg acaatacacc cgacacctac
     3841 cgttccaata tgccggcagg cacggtgaac gtcggcattg cccgaatccg acccgccgcc
     3901 gcacagacag cggacattac cgtcgcacct ttgccgcaga aaaccgtccg tacggaaccc
     3961 gatccccttg tccgtattgc cgaacctgcc cttgcgacag ccgcagcgca acctcaaacc
     4021 gaaaaacaga ccgccatgcc gtctgaaacc caaaccgcaa cactcgcgca gatcatcccc
     4081 caaaacgaca tgcaggcggc agacgaactc atgcagcttg ttgcccgaaa caacctgcgc
     4141 cgacaggctg aagaaaccat ctccgccgtc atcggcacgc ctgacacagt tgccgaacac
     4201 aacatttcct catctccgca acataccgtt gccgccgacg gcaaacgccg ggcacgtttg
     4261 gaaacgcgcg tagccaaagc tgccgacggc gaagccgaaa tctccccgct ccatgccagc
     4321 atccaccgcg ttgtagaagg cgacaccctg ttcaacattg ccaaacgcta caacgtcagc
     4381 gtagccgacc tgattgtcgc caacaacatc aaaggcaaca ccatccaaaa aggacaggta
     4441 ctccgcctgg cgcaggcagc ccctgcccaa acccgtattg aaaaagtatc ctacaccgcg
     4501 cgcaaaggcg acaccttcaa aagtatcgcc gcgcgcttca atatccatat cgacgacatc
     4561 cgccggctca atcccaacct gaacaccatc aatccgggac agagggtcaa actgattgga
     4621 agctgattcg gatacggcac atgacaggac tttctcagtc ctgtcttttt catcccacat
     4681 ttcccatacc atcatgaaac tcatcaaata cctgcaatat caaggcatag gaagccgcaa
     4741 gcagtgccaa tggctgattg ccggcggtta tgttttcatc aacggaacct gtatggacga
     4801 caccgatgca gacatcgatt cctcatctgt cgaaacgttg gatattgacg gggaagcagt
     4861 aaccgtcgtt cccgaaccct atttctacat cctgctcaac aagcccgaag attacgaaac
     4921 ttcgcacaaa ccgaaacact accgcagcgt attcagcctg ttccccgaca atatgcggaa
     4981 catcgatatg caggcggtcg gcaggctgga tgcggatacg accggcgtat tgctgattac
     5041 caacgacggc aaactgaacc acaacctgac ttcgccgagc agaaaaattc ccaagctata
     5101 cgaagtaacg ctcaaacacc ccacaggaga aacgctctgc gaaaccctga aaaacggtgt
     5161 cctgctccac gacgaaaacg aaacggtttg cgccgccgat gccgtgttgg aaagcccgtc
     5221 cgcattgctc ctgatcatta ccgaaggcaa ataccaccaa gtcaaacgga tggtcgccgc
     5281 cgcaggcaac cgcgtgcagc atcttcaccg ccggcgattc gcacatttgg aaacagaaaa
     5341 cctgaaaccc ggcgaatgga aatttatcga ttgtccgaaa ctctaaaata acatccaaga
     5401 attccattta tattcatcca catactcatt aaatatatgg tttaacccaa tttaacgcaa
     5461 aaaatcaatt tacgatataa tccatttgtc tgagtaacgc ctgttcaagc aggcttatga
     5521 gtaagacgtt ttccccgtaa tgtgttttgc catctatact tctccccttg tatagatggt
     5581 tttttatagt ggattaaatt taaaccagta cagccttgcc tcgccttgcc gtactatttg
     5641 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata tttcaaattc
     5701 cctctctttc atcattattt aaatgctatc attaatataa ttaacaaaat ttcatttcca
     5761 tacaccgatg tagaagaata acaaattaat ctatttataa actccgattc ttcctcatcg
     5821 ggttatactc atcagaaata ctcactgaaa tatatgcctt ttacatggaa aatcactaga
     5881 cagttgaaaa acaattagtc agttcctgtt aaaaaacgcg tgtagaattt ccgctttatc
     5941 tcaatcggac acggtttcaa tatgtcttcc ccttcaaata ccaatcgtca aacctgatcc
     6001 agccgattaa cctatatcct gaccgttgcc ggcgcgactg tcggcttcgg cgcgacgtgg
     6061 cgttttccct accttgtcgg cgaaaacggc ggcggcgcgt atgtgttttt attctgtatc
     6121 gcgatgctgg ttatcggcat cccgatgatt ttggtggaaa atgtcatcgg acggcgcaaa
     6181 ggcgtgaacg cgctggatgc gttcggcggc ccgatgaacg gcaaacccat tgccaaaatt
     6241 tggaaactgg tcggctggat gggctgctcg gcgcgttcgg catcatggct tattacatgg
     6301 tgctcggcgg ctgggttatc agctacattg ttaatatcat cggcggtaat ttggacattt
     6361 ccagtcctgt ttccagcgaa gtcaccaaaa gtttctttac cgaacacatc gaaaacagtc
     6421 cttgggaaat tgctttctac accttcctat ttgtggttgt gaaccaatgg atcttggtaa
     6481 aaggcgtgat cggcggtatt gaaaaagcgg caaaatacct gatgcctttg ctgtttttat
     6541 tcctgattgc catggttgtg cgcaacgtta ccctgccggg cgcaatggaa ggcattacct
     6601 tctatctgaa acctgacttc agcaaaatta cagccgaact gttcgtcttc gttttaggac
     6661 aagtgttctt taccttgagc ttgggtttcg gcgtgatgat taccttgtcc agctatctgg
     6721 ataagaacga aaatctggtg caaaccgcgg tcatcacggc cattaccaat accatcatcg
     6781 ccgtactggc cggctttatg attttcccgt cgctcttcag cttcggcgtc gcgcccaatt
     6841 ctggcccgac tttggtattc caaagtttgc cgattgtgtt ctcacatatg tgggcaggac
     6901 ctgtgttcgc tgtcattttc ttctctctgc tcctgattgc cgcgctgaca acttcgctga
     6961 cgatttatga agtgttgatt acgaccattc aggaaaaaac caaaatccgc cgtaccgccg
     7021 caatcacgat tgtattgtcc gccatcttca tcttcggcaa tattccgtcc attctgagct
     7081 atggcccgtg gaaagatgtt tccatatttg gcaaaaatat ttttgacgct tttgactaca
     7141 tcagcggcaa catcttgttt atgctgaccg cgctcggctc cgcgctgttt gtcggttttg
     7201 tgatgaagga tgaagcgaag gacgaattgc tttataaagg caaccatata acggtcaata
     7261 tttggtttgc ctatgtgaaa taccttgtgc cgctggtgat tttgctgatt ttcgtcagca
     7321 acctgttcta atccgcagca atcgatgccg tctgaaagtc atacctcttt cagacggcat
     7381 tgcgttgatg cccgccacaa tacccgcaac gccagaatcc gaaccggctt gccggcttct
     7441 gtcggatatt tccgggcagg cggcgttccg tctgcttaga caatcgtcct ttaaaacagg
     7501 tagaatctgc cccaacggga aacacatcct tcagacggca aaacccatac cccaaaccat
     7561 caggaatccc ccttatgaac agacaaaaag tcatcgccat cgacggcccg ggcgcatcgg
     7621 gcaaaggcac ggttgccgcc cgcgttgccg ccgcattggg atacgattat ctcgataccg
     7681 gcgcactcta ccgcctgacc gccctatatg cacaaaaaca aggcgtggaa tggcacgatg
     7741 aagaaaacgt ttccaaactg gcaaaaaaac tgcccgccgt attttcaggc agccgcatcc
     7801 tgctcgacgg cgaagacgtt tcagacggca tccggacaga agccatcggc atgggcgcat
     7861 ccgcagtcgc acagttgcct aaagtccgcg cctccctgct gcaacgccaa cgcgattttc
     7921 tgaccgaaaa aggactggtc gccgacggac gggacaccgg atcggtcgtc ttcccccagg
     7981 ccgaactcaa aatttttctg acagccgaat ccaaaatcag ggccgaacgc cgcgccaaac
     8041 aaatcggcat cccctgcgaa ggtttggcat tcgagcgcat cctgtccgac atcgaagcca
     8101 gagacgaggc agaccgaaac cgcaaggttg cccccctgaa acaacaaccc gatgccctgc
     8161 ttttggatac gagccgcctg actatagaag aaactgtaaa aaaagtgctt gattggtatc
     8221 gtaaagttta aattttcagg tataatcgcc cgcattgcgt ttcagacagc atgaatttcc
     8281 ccatatgccg tctgaaacct tatttacccg tctgccctgc caaggacggc agacacccac
     8341 caacccaccc cgcacccctt ggcggtgtac cgaaaagagt tatatatgtc tatggaaaat
     8401 tttgctcagc tgttggaaga aagctttacc ctgcaagaaa tgaacccggg tgaggtgatt
     8461 accgctgaag tagtggcaat cgaccaaaac ttcgttaccg taaacgcagg tctgaaatca
     8521 gaatccctga ttgatgtagc tgaattcaaa aacgctcaag gcgaaattga agttaaagtc
     8581 ggcgacttcg ttaccgttac catcgaatcc gtcgaaaacg gcttcggcga aaccaaactg
     8641 tcccgcgaaa aagccaaacg cgctgccgat tggattgccc tggaagaagc catggaaaac
     8701 ggcaacatcc tgtccggcat catcaacggc aaagtcaaag gcggcctgac cgttatgatt
     8761 agcagcatcc gcgcattcct gccgggttct ttggtcgacg tacgtcctgt aaaagacact
     8821 tctcacttcg aaggcaaaga gatcgaattc aaagtgatca aactggacaa aaaacgcaac
     8881 aacgtcgttg tttcccgccg cgccgttctg gaagccactt tgggtgaaga acgcaaagcc
     8941 ctgctggaaa acctgcaaga aggctccgtc atcaaaggca tcgttaaaaa cattaccgat
     9001 tacggtgcat tcgttgactt gggcggcatc gacggtctgt tgcacatcac cgatttggca
     9061 tggcggcgcg tgaaacaccc gagtgaagtc ttggaagtcg gtcaggaagt tgaagccaaa
     9121 gtattgaaat tcgaccaaga aaaacaacgc gtttccttgg gtatgaaaca actgggcgaa
     9181 gatccttgga gcggtctgac ccgccgttat cctcaaggca cccgcctgtt cggcaaagta
     9241 tccaacctga ccgactacgg cgcattcgtc gaaatcgaac aaggcatcga aggtttggta
     9301 cacgtctccg aaatggactg gaccaacaaa aacgtacacc cgagcaaagt cgtacaactg
     9361 ggcgacgaag tcgaagtcat gattttggaa atcgacgaag gccgccgccg tatctctttg
     9421 ggtatgaaac aatgccaagc caatccttgg gaagaatttg ccgccaacca caacaaaggc
     9481 gacaaaatct ccggcgcggt taaatccatt accgatttcg gcgtattcgt cggcctgccc
     9541 ggcggcatcg acggtttggt tcacctgtcc gacctgtcct ggaccgaatc cggcgaagaa
     9601 gccgtacgca aatacaaaaa aggcgaagaa gtcgaagccg tcgtattggc aatcgacgtg
     9661 gaaaaagaac gcatctcctt gggtatcaaa caactggaag gcgatccgtt cggcaacttc
     9721 atcagcgtga acgacaaagg ttctttggtt aaaggttccg tgaaatctgt cgatgcgaaa
     9781 ggcgccgttg tcgccctgtc tgacgaagta gaaggctacc tgcctgcttc cgaatttgca
     9841 gccgaccgcg ttgaagattt gaccaccaaa ctgaaagaag gcgacgaagt tgaagccgtc
     9901 atcgttaccg ttgaccgcaa aaaccgcagc atcaaacttt ccgttaaagc caaagatgcc
     9961 aaagaaagcc gcgaagcact gaactccgtc aatgccgccg ccaatgcgaa tgccggcacc
    10021 accagcttgg gcgacctgct gaaagccaaa ctctccggcg aacaagaata aggttgcaga
    10081 catgacaaag tctgagttaa tggttcgttt ggcagaagtg tttgccgcca aaaacggcac
    10141 gcatcttctg gcaaaagacg tagagtacag cgtaaaagtc ttggttgaca ccatgactag
    10201 atcgcttgcc cgaggtcaac gcatcgaaat ccgcggtttc ggcagcttcg atttgaacca
    10261 tcgtcctgcc cgcatcggtc gcaatcccaa aaccggcgag cgtgtggaag tacctgaaaa
    10321 acatgtaccc cacttcaagc ccggtaaaga attgcgcgag cgggtcgact tggctttaaa
    10381 agaaaatgcc aattaaacct tagcatcaaa acgccgctgt tacgcggcgt tttttctgtg
    10441 gtttaacttc atccgctgct tcaatacctt gagccaagca agcaacggat tagagcgtgg
    10501 at
//
LOCUS       DAWHXZ010000054        10221 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000054 DAWHXZ010000000
VERSION     DAWHXZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10221
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.054"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1440
                     /locus_tag="WIE35_000868"
     CDS             <1..1440
                     /locus_tag="WIE35_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ4153803.1"
                     /translation="PLAIAAVLCALVLALPVPDGVKPQAWTLLAMFIGVIAAIIGKAM
                     PLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMISRGLLKTGLG
                     MRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQSIAGSYGSN
                     PAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSSFRLSWGAWA
                     WAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSADEIIMAVIFG
                     ILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEKSAWDTIIWF
                     GALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAHYMFASTTAH
                     ITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFGSGYTTMGEW
                     WKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(1647..2723)
                     /locus_tag="WIE35_000869"
     CDS             complement(1647..2723)
                     /locus_tag="WIE35_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ4153804.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            2946..4088
                     /gene="bioF"
                     /locus_tag="WIE35_000870"
     CDS             2946..4088
                     /gene="bioF"
                     /locus_tag="WIE35_000870"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ4153805.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFSRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAVCL"
     gene            4098..4745
                     /locus_tag="WIE35_000871"
     CDS             4098..4745
                     /locus_tag="WIE35_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ4153806.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELIALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            4733..5515
                     /gene="bioC"
                     /locus_tag="WIE35_000872"
     CDS             4733..5515
                     /gene="bioC"
                     /locus_tag="WIE35_000872"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ4153807.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            5735..7171
                     /locus_tag="WIE35_000873"
     CDS             5735..7171
                     /locus_tag="WIE35_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ4153808.1"
                     /translation="MENSLIIGLALAVLLVLLVMRAKQGKKTPKRKSQGVGNTQGQPP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQYADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            7174..8271
                     /locus_tag="WIE35_000874"
     CDS             7174..8271
                     /locus_tag="WIE35_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ4153809.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            8283..8819
                     /locus_tag="WIE35_000875"
     CDS             8283..8819
                     /locus_tag="WIE35_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ4153810.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            8821..9465
                     /locus_tag="WIE35_000876"
     CDS             8821..9465
                     /locus_tag="WIE35_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ4153811.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRA"
     gene            9488..9847
                     /locus_tag="WIE35_000877"
     CDS             9488..9847
                     /locus_tag="WIE35_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ4153812.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            9940..10015
                     /locus_tag="WIE35_000878"
     tRNA            9940..10015
                     /locus_tag="WIE35_000878"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9973..9975,aa:Asn,seq:gtt)
BASE COUNT         2621 a         2182 c         2646 g         2772 t
ORIGIN      
        1 cccctcgcca ttgccgcagt attgtgcgcc ttggttttgg cactgcccgt acccgacggg
       61 gtcaagcctc aggcttggac gctgctggcc atgtttatcg gtgtgattgc cgccattatc
      121 ggcaaggcca tgccgttggg tgcgctgtcg attattgccg tcgggttggt cgcagtaacc
      181 ggcgtaaccg ccgacaaacc gggtgcggcg atgagcgatg cgttgagtgc gttcgccaat
      241 ccgttgattt ggctgattgc catcgcagtt atgatttcgc gcggtttgct caaaacaggg
      301 ctggggatgc gtatcggata tttgtttatc gccgtttttg gaagaaaaac gctgggcatc
      361 ggttacagtc tcgctctttc cgaactgctg ctggctcccg ttaccccttc caataccgcg
      421 cgcggcggcg gcattataca tccgattatg cagtcgattg ccggcagtta cggctccaat
      481 cccgcaaaag gcacagaagg caagatgggt aaatatttgg ctttggtcaa ctatcattcc
      541 aatcccattt cgtcggctat gtttattact gcaactgccc ccaacccttt aatcgtcaac
      601 ttgattgccg aaaatttagg cagtagtttc cgtctttctt ggggggcgtg ggcgtgggca
      661 atggctgttc ccggcgttat cgcctttttc gttatgcctt tgattttata ttttttgtat
      721 ccgcctgaaa ttaaagaaac gcccaatgcc gttcaatttg ccaaagaccg tctgagggag
      781 atgggtaaaa tgtcggcaga cgaaatcatt atggcggtca ttttcggtat cttgttgctg
      841 ttgtgggcag atgttcccgc ccttattacc ggcaatcacg cttttagtat caacgccacc
      901 gccaccgcat ttatcggatt aagcctgctt ttgctttccg gtgtattgac ttgggacgat
      961 gttttgaaag aaaaaagcgc gtgggatacg attatttggt ttggcgcatt gattatgatg
     1021 gccgcatttt taaataaact cggactgatt aaatggttct ccggagtgtt ggcggaaagt
     1081 gtcggcggtt tgggcgttag cggcacggct gcgggcgtaa tcctcgtgct tgcttatatg
     1141 tatgcgcatt atatgtttgc cagtactact gcacatatta ccgctatgtt cggcgcattt
     1201 ttcgctgctg ccgtttcact gaatgccccg gcgatgccga ccgcgctgat gatggcggcc
     1261 gcatccaaca ttatgatgac cctcactcat tatgcgaccg gtacttcgcc tgtgattttc
     1321 ggttcgggct acaccacaat gggagaatgg tggaaggcgg gttttatcat gagcgtggtc
     1381 aattttctga tttttttcgt tatcggcagc atttggtgga aagttctggg gtattggtaa
     1441 gggaaaaata aaataaattt ccaatctgtg tttatttgat tgggcgacta ttatcgtgaa
     1501 atatgccgtc taaagccttc agatggcata tttgtgcgct tgaatgttgc agaaagcggc
     1561 aggcggcggt gtaggaaaag ccaaacaaaa accaaaccgc ctatcaactt ctgataaaca
     1621 taagcattaa ataatcagaa ggttattcaa ttacctaaac gcaaatttcc ctgccgtatc
     1681 acatctattg aaaataatac atcaaccggc tcggaagcag cctgatcagg tgtttctact
     1741 tgcggcgatg aatcggcagc cggttcggta taggcagtcg gcgtgccgtc ggattgggta
     1801 tttaaatgcg gttgtgcctc gggcgtgtgt acatcaggca cttcgggcgt gcgtgtctcg
     1861 gatatttcgg cagagttggt ttcctcagtt tgttcaatga cttcagcttg gctgtatgag
     1921 gaagaaccct gtatccacgc cagcgatttg agcggcatct tcatcttgcc gtttttgccg
     1981 caggtcaggc agacggccga tccggtgcgg tctttgagta cagcatcgac tttctcgggc
     2041 gcgatggttt taccctgtgt ttttgcccat tgcgcaatac tttttttcag agaggcgatg
     2101 tcaaggttgt tcttaccgta agggtcgcgg aaggcggcaa gccacaggtt gcgatccgca
     2161 tcttcattga gccccgccat ttgatagatg acggaggcgg gcgagccgct ggcgatggtt
     2221 ttggcaaact cgagcgcgtc gggcgatttc atgcggacgc agccgtgact ccgaaccccg
     2281 gggacgctgg ccggcgcatt ggtcccgtgt atgcccaaac cgagtttggg gtcgcctaag
     2341 cggacaaaaa ccggccccaa agggttgtcc gggccggcgg ctatggtttt tacgccgtcg
     2401 ccgcgttctt tctgtatgga tttggggatg taccaaacag ggttataggc tttcgcaccg
     2461 attttatgtt cgcctagatt ggtttgcgtc atcgcccgac ctactgcaac gggataaacc
     2521 ttggtcagtt tgccgtcggt gtagaggaac aggcgttgct gagggatgtt aatgaagaca
     2581 tgttgacctt gtgcgacggg ggagacatcg ggaatgacgg tgtttgcgta tgaaaaaccg
     2641 cttatcaata gtgcggcagt gcggcagatt gttttattca tatcaaaata tggtgtgtgt
     2701 ccgataggtt ttcggcaaat catacctgaa accgtaccaa tttgtgcgaa aatatgcgct
     2761 tcggtacagt gcggacggat tgggtaatgg caacggaaac aaatgtcgcg gaaatttccg
     2821 ccttggatta tgaaggcagg ggtgtggcaa aggtcggcgg caaaacggtt tttattaaaa
     2881 gggcattact tgattgtttg atgctgggtt ggttcaggct ttaactcagg aatatttaca
     2941 tcataatgaa ggtttttaaa caacagcttg aacaactcgg cgcgcaaaac caatatcgtt
     3001 cgattccgga tttgattcat caagggcggt atattacgcg ggaaaactgc aaaatgctga
     3061 atatgtcgtc taatgattat ttgggtttgg catcagatga aaacttgcgc cggtcttttt
     3121 tgcagcaata cggcggtaat tttccctctt ttaccagttc ttcatcgcgt ttattaacgg
     3181 gcaactttcc tatttatacc gatttggaag agcttgtcgc acaacgtttc caacgggaaa
     3241 gcgcgttatt gttcaacagc ggctatcacg ccaatctcgg tattttgcct gctttgacga
     3301 cgacgaaaag tttgattttg gcagataaat ttgttcacgc cagtatgatt gacggcatcc
     3361 gtttgagccg gtgtgcgttt tcccgttatc gtcataatga ttatgaacat ttgaaaaatc
     3421 tgcttgaaaa aaacgtcgga aaatttgacc gcacttttat cgttaccgaa tctgttttca
     3481 gtatggacgg cgatgtggcg gatttgaaac agcttgtcca attaaaaaaa cagtttccca
     3541 atacttatct ttatgtggat gaagcccacg caatcggtgt ttatgggcaa aacggattgg
     3601 ggattgccga acgggataat ttgattgccg agattgattt attggtcggc actttcggta
     3661 aagccttagc ctcggtgggg gcgtatgccg tctgcaacca agtattgaaa gaatgtttga
     3721 ttaatcaaat gcgcccattg attttttcaa ccgcattgcc gccgtttaat gtggcttgga
     3781 cttattttat ttttgaacga ttgccgcaat tctcaaaaga aagaagccat cttgagcagt
     3841 taagcgcatt tttacggcgg gaagtggcgc atcggacgca aataatgccg agccaaacct
     3901 gtatcgtccc ctatatttta ggcgggaatg aagccaccct tgccaaagcg gaatacctgc
     3961 aaaggcaggg ttattattgc ctgcccatca gaccaccgac agtacccaaa aacacatcca
     4021 gaatccgcct gtctttaacg gcagatatga caacggatga agtgcggcag tttgcggtgt
     4081 gcctgtaagg atatgatatg aaaacaaaat tttacaatca tcaaggcgga catttaatcc
     4141 tgtattttgc aggttgggga acgccgcccg atgctgtaaa tcatttgatt ttgccggaaa
     4201 atcacgattt attgatttgc tatgattatc aagatttaaa tttggatttt gatttttccg
     4261 cctatcggca catccgtttg gtggcgtggt caatgggcgt ttgggcggca gagagggcat
     4321 tgcaaggaat aagattaaaa tccgcaacgg cagtgaatgg cacaggtttg ccttgcgatg
     4381 ataatttcgg tatcccttgc gccgttttta aaggcacatt ggagaacctc acggaaaaca
     4441 cccgtttaaa atttgaacgc agaatgtgtg gcgataaagc atcttttgaa gattaccaac
     4501 aatttcccgc ccgtccgttt gacgaaattc atcaagaact tatcgcactt tttgcgatga
     4561 tcgggcaaga taggcgcgca gatttgattc gctggacaaa tgccttggtc ggatcgggcg
     4621 ataaaatttt tatgcctgcc aatcagcacc gatattggac accgcgttgc accgttcggg
     4681 aaattgacgg cggacattac ctgttttcaa gattcaccca ttggtcggca ctatggaatc
     4741 actgactgcc ataaataaat cgcgcattcg gcaggctttc caaaaagcat taaacgatta
     4801 tgaccggcac gccttaatcc aacaaaaaat gacgattaat ctaatggcgc atttgcaaga
     4861 ttatttgccg gatatgccat tggaaaacgt gttggaattg ggctgcggct caggaatgtt
     4921 gagtgccttg ctgcaaaaac agatttcagc gaattattgg ttatttaatg atttgtgcga
     4981 tgtgcagccc caactggctg aaaaactgcc gcaatccttt gatttttatt gcggcgatgc
     5041 ggaaaacttt ccttttcaac gacaatttga cttaatcgca agcgcatctg ccgtgcaatg
     5101 gtttcatcaa cccgacgctt ttatcgccca ttgcaaaaca ggcttgaaag caaacggatt
     5161 attggcggtt gcaacctttg gcaaagacaa tttaaaagaa gtccgccaaa ttacaaatat
     5221 aggcttaaat tacccgactt tatcccaatg gcagacttgg ttagccaaag attttgagct
     5281 tttatggtgt gaggatttta aggtaatact agactttgat acgccgttag atgtactcaa
     5341 acaccttaaa tatacaggcg taacagccac gaaccaaaaa aattggacaa gaaaaaatct
     5401 caatggattt attggcgatt acttgtcggc gttcagtatg ccgtcgggca aagtgcgcct
     5461 gacttatcat ccgttatttt ttatcgcgcg ctactctgcg gcgggcaggc aataaggcag
     5521 cttatgggca aagttatttt tatatcgggt attgatactg atgtgggtaa aaggtaatat
     5581 ggcgaggctt gtgcagaagg catattgtta aacgttaaat tatggtatga tttaaaactt
     5641 acaagtctat ttcagtaaat cgttaataat aaaagcggac aatggccgtt gcaggcggtc
     5701 agctatccgc tcgttacggt atcgggagta tcctttggaa aactcattaa ttatcggtct
     5761 ggcactggcg gtattgctgg tgcttttggt tatgcgtgcg aagcagggta agaaaacacc
     5821 gaagcgcaaa tcccaaggtg tcggcaatac gcaggggcag ccgcccggaa gcaatgattc
     5881 ggactgggtc gatcagattg gacaatcggt ttcagacggc acgcaacccg actggtcttg
     5941 gaacgaaagt gccgagaccg catccgccgc cgtatccgcg caagaagtcg atccgcttac
     6001 ggagtatcag gtttataagc aattcggtta tcagggcaag gctgccgaat ctttggctgc
     6061 ctatctggac ggcattccgg atggtgaagc gaaacctgaa aaccttatcc gcgagctgct
     6121 cgatatcaat ctcgaagtgg gggatgtcga tgttttggca gacaatctgc aaaaatacgg
     6181 caaactgatt ccttccgaac ttttggcaaa atatatcgaa caggcattac agcgcgattc
     6241 aaaccatttg cgtatccgcg tcttggcgga agaaggtttg ggatggggta ctcaggagat
     6301 tgaaaaacgt gcggaaggcg gttctgcgac ggcagcttcc gcatcgcccc cgccggatgc
     6361 cggcggtaag gcttatgaag ccgaagaaat caagcgcatc ccgattgtgc ggggcaaaaa
     6421 agacgtgtcc ggaatcagtc aagaggaaat cggtgcgatt gccggtttgg tccgtgccga
     6481 tcaaggtgcg aaaatcctta aagacaaagt cagctatgaa acggcatcga aacaatacga
     6541 ccgtgccatc caaacttccg aaaaacctgc aaacctgatt atcgatgcgt tgaaactcga
     6601 ttaccaatac gcggacatag accgttttgc cggacatttg tggaaacttt accaaacgtt
     6661 gggcaactac ggcaggcagg ttaaagagcg gatgctgggg tgggggtaca gcctgggtta
     6721 ccatgaggtt ttcgatgatt tggaaaaagg gccgaacgac cggcaaatca aagacatcgg
     6781 tatggggcac gggtatctgc cgaaaaatat acagaaattc aaatcgcaac atcgggattt
     6841 ggtgcttcaa gattcttcgt tgatcaacac cggttcgtct ccggcagacg atgcggttaa
     6901 ggaagtagag tcgttgctga tgtatggtca gattgaagcg gcaatggatg tgttggagca
     6961 ggcggtattg aaatatcccg acgagtccca gctttatatt acgttgatcg atatttatga
     7021 acgtactgaa gattgggata ggttggggca gtttttaagg gtattgaggg aacgtgcgga
     7081 caggcttcct gaagaggtcg ttatgctgat gagccggctg ctgcagcgta tgaatcaaaa
     7141 tattaaaaaa ataaaacggt acggaaaata aaaatggaag ttcaactgcc gaaaattaaa
     7201 acagtacgcg taatgttggc ggggatgacg gcgcagcagg aatccgtttt caaaatggca
     7261 ttcaaaatgc acaataccac ccgttatgaa acagtatccc cttcagacgg cagtgccgtg
     7321 cccgatttgg ttttggcgga taccgatgcc gagggcggtt ttgaactttg gaaagagctt
     7381 gccgagcgtt ataaggatat acccgtcgcc gtctgttcgg agaaagttcc cgattctgaa
     7441 gttccctacc tgcccaaacc gattcggttt gaaacattgt ttcctatgct ccgcaaattg
     7501 ttgcagggcg agaatgttta tgggaaatcg tttattgcac ccgcagaccg gtcggcgaaa
     7561 aataacggga atgtgcagcg tacggttacg atacgccagt ttaacccgaa taaaggatta
     7621 ttgggggcgt tgcggtttgc ggaaaagaac aggcaggaca tcgctatctt gcatggaaat
     7681 aagccggtcc ttattgtttt cccctcgata caacgggttt tgctgacaga aagtgtgcaa
     7741 aaactcgaag aattgtgcaa agacgaaaat ttgcaggtca gctgcaagac tgttcccgat
     7801 aacccgcaat ggcgcgaaaa ggctaaagta ggcattatgt cctgtatgtg gcagttttcc
     7861 atttggacag cgcagggcag gttgatttat ccgatttctc ccgatactcc gtttacgttg
     7921 aaatcttggc caaacctgac ccggttggca aatgtgccgg ggtcgatacg cttgtcggca
     7981 tttctgacca aggcatccgt caaccttaac gtgttgtata aagtgatgcc tttaaacctc
     8041 aatgatattc tgaattatct tgcggcaacc tatacaaccg ggtttttgtc ggtagattta
     8101 aaaacggttt cacaacaggc atactccgat atggcggata aaataaatat cggagccgat
     8161 tctgcctctg atagtgaaat gatgaaaaaa gcggaaaaaa tcacaacacc atcccaatcc
     8221 cagtcgcgcg gccttctgca aaggctgatg aaaaaactgt tgggcagcta agaggcggag
     8281 agatgagaga aaataaaatt attttcacag gacctgtcgg cgtagggaaa accactgcca
     8341 ttgcggctat ttcggacgaa gcactcgttc agaccgatgc ttccgcatcc gatatgactt
     8401 tggataggaa aaggaatacg acagtggcga tggactacgg ggccatcagc ttggatgagg
     8461 ataccaaagt ccatttatat ggtacgcccg gtcaggaacg gttcaacttt atgtgggaaa
     8521 tcttaagcca aggcagtatg ggtttggtct tgcttttaga taatgcccga accaatccgt
     8581 tgaaagattt ggaattcttt ttacattcgt ttcgagggct gctggagaag gcacccgtcg
     8641 ttgtcggtat taccaagatg gatatacgct ctcagcccgg tatcgacgtg tatcacaaat
     8701 atcttgcaaa acataatctt aatgttccgg tttttgaaat tgatgcccgt aaggaagatg
     8761 acgtaaaaca attggttagc gcaatgttat tttctattga tccgggactg gaggtttaat
     8821 atggaatcaa cactttcact acaagcaaat ttatatcccc gcctgactcc tgccggtgca
     8881 ttttatgccg tatccagcga tgcccccagt gccggtaaaa ctttgttgca cagcctgttg
     8941 aaagcagatg cggacgaaat ggtcagcagt gagaagctgc ttacttgggc ggacaccgcc
     9001 gacatcgata ccgctttgaa cctgttgtac cgtttgcaaa aactcgaatt cctctatggc
     9061 gatgaaaacg gtcattcaga cggcatcaat ttgtcggacg agcaattgcc gttgctgatg
     9121 gaacaattgt ccggcagcgg taaggcgtta ttggtcgatc ggaacggtct gtatcttgcc
     9181 aacgccaatt tccatcatga ggcggcggaa gagttggggt tgttggcggc agaagtcgca
     9241 cagatggaaa agaaataccg gctgctgatt aagaacaacc tgtatatcaa caataacgct
     9301 tggggcgttt gcgatccttc cggtcagagc gaattgacat ttttcccatt gtatatcggt
     9361 tcaaccaaat ttattttggt tatcggcggc attcccgatt tgggcaaaga ggcatttgtt
     9421 actttggtaa ggattttata ccgccgttac agcaaccgcg cgtaaaactt gggagagagg
     9481 aggggttatg cagcaattat tgatttcaat ccttgaagat ttaaacaata catctacgga
     9541 tattatcgcg tctgccgtta tctcaaccga cggattgccg atggcgacaa tgcttccttc
     9601 acatttgaat tcggacaggg taggggcgat ttctgccact ttgcttgctt tggggagtcg
     9661 ctcggtgcag gaactcgcct gcggggaatt ggaacaagtg atgattaaag gaaaatcagg
     9721 ctatatcctt ttaagtcagg cgggtaaaga tgccgtgttg gtgctggtgg caaaagaaac
     9781 cggcagactt ggtttaatcc tattggatgc caaacgtgcg gcaaggcata ttgcggaagc
     9841 catataacat ataaagattg cgggcttgca gataaagtgc aatcgattgt caatttatat
     9901 tgacacgttc ggtatttctg ttttattatt cgcgcttgtt ccccgatagc tcagtcggta
     9961 gagcgacgga ctgttaatcc gcaggtccct ggttcgagcc caggtcgggg agccaaattt
    10021 caaaaccctc taagtatttt cttagagggt tttgttttac cggcggtcag aaacgcattt
    10081 ttgagatgat tgttttgaga tggaataaaa tctttgcaaa attcctttcg tgatggttat
    10141 gaaaaaatag gggctgtcct ggacagctag gataaactcg attttatagt ggattaacaa
    10201 aaaccagtac ggcattggct c
//
LOCUS       DAWHXZ010000055        10037 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000055 DAWHXZ010000000
VERSION     DAWHXZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10037
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.055"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..309)
                     /locus_tag="WIE35_000879"
     CDS             complement(<1..309)
                     /locus_tag="WIE35_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153813.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     Q"
     gene            complement(364..1353)
                     /locus_tag="WIE35_000880"
     CDS             complement(364..1353)
                     /locus_tag="WIE35_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ4153814.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEERE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(1476..4178)
                     /gene="ppc"
                     /locus_tag="WIE35_000881"
     CDS             complement(1476..4178)
                     /gene="ppc"
                     /locus_tag="WIE35_000881"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ4153815.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESCGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            4221..5114
                     /locus_tag="WIE35_000882"
     CDS             4221..5114
                     /locus_tag="WIE35_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ4153816.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALADRLKRINYNVDVRTVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGAER"
     gene            complement(5515..5739)
                     /locus_tag="WIE35_000883"
     CDS             complement(5515..5739)
                     /locus_tag="WIE35_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ4153817.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDTQEPYSLRDEIPPHY"
     gene            complement(5740..7128)
                     /locus_tag="WIE35_000884"
     CDS             complement(5740..7128)
                     /locus_tag="WIE35_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ4153818.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGMMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(7214..8035)
                     /gene="prmC"
                     /locus_tag="WIE35_000885"
     CDS             complement(7214..8035)
                     /gene="prmC"
                     /locus_tag="WIE35_000885"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ4153819.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(8042..8485)
                     /locus_tag="WIE35_000886"
                     /pseudo
     CDS             complement(8042..8485)
                     /locus_tag="WIE35_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8550..10034)
                     /gene="tldD"
                     /locus_tag="WIE35_000887"
     CDS             complement(8550..10034)
                     /gene="tldD"
                     /locus_tag="WIE35_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ4153820.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVRERLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
BASE COUNT         2074 a         2824 c         2835 g         2304 t
ORIGIN      
        1 ttgggggagg cgcgctgcca gtgcgctgat tttttccctg ctctgttcga gcaggctgcg
       61 gtatcgtgta ttttcttctg tcaggctgtc gattttgttt tgcaggtctt ctttgagttt
      121 gccgacttgg acgagcaggg cttcgctgag ttcgtcgacg gcggtttcgt gttcgagttt
      181 ttgccgctcg tgcgcccgtt tgagttcggc gacggtttct ttgaggcggc ggttttcgct
      241 gacgagggtt tcgaattttt gtaccagcgt ataaacgctg ctttcgagtt tttcgatatt
      301 ttgtttcata accttacctg tccgtatgcc gtctgaaggc ctcagacggc atctgtctgt
      361 tgtttattcg aaacgcgcgc tgcgttccat cagtctttcg acgacctgct gaggggtcat
      421 ttctttgcgg atgagttgca gcagggtttg ggtaatcggc atgtcgattt ggtacttaca
      481 ggcagtattg aagacttctt ctatcgtgct gaccccttcg gaaacgtgtc cgatttcgac
      541 cagcacctga tgcagttcct tgccttctgc caaacccaag ccgacgcggc ggttgcgtga
      601 gagtgcgccg gtgcaggtca agatgaggtc gccgatgcca gcaagcccca tcatggtttt
      661 gggctgtgcg cccattgcgg aggcaaggcg ggtgatttcg gccagtccgc gtgtaaccag
      721 tgcggcacgg gcgttaagcc cgtactccag accgtcggac aatccggtgg caatcgccat
      781 gacgtttttg actgcaccgc cgactgccac gccgataacg tcggtactgc cgtaaagcct
      841 catgacggtc gtgttgagct gcggtacgag ttcttcaatc cactcttggt tttcggaggc
      901 gaggacgacg gcgcagggca gttgtttggc gagttcctgt gcaaaactcg gaccggaaag
      961 tacgccgatt tttttattgt cgggcaatac ttctttcaat acttgaaagg tcagcagccc
     1021 tgtatcctgc tcgaatcctt tgcaggcggc gaggacgggg aggtgtcccg cgccgtactg
     1081 tttgagcaac tctgcgctgc ttctcaatcc ggcaacggag gttacgataa ggacaagtcc
     1141 gctgtctttg agcgcgtctg ccaaatccgc acacacttcc aaggtttcgg gaaaggaaaa
     1201 gccgggcagt ccgcgtttgt tttcacgttc ttcctgcatt tggcggactt ggtctgcgtt
     1261 gcgcgtccac agggatacgc ggttgccgtg ttgggaaaaa tgcagggcga gcgccgtacc
     1321 ccacgaacct gcgccgataa cggtaatttt cattggtcgt ctttcaacat atcactgccg
     1381 ttcactttaa aacaatcggt gtttctctgc aagtgcggtc agggaaatgc cgtctgaaag
     1441 gcgttcagac ggcattttgc cccgatgcgg cactatcagc ctgtattgcg caaaccttgc
     1501 gccacgccgt tgatggtcag gtgcaccatc agaagggcgt gcggattgtc gggttcttta
     1561 cgcaggcgtt tgagcatggc gacttgcagg ccgttgaggg cgttcaaata tggaatcctc
     1621 aaggcaagcg aacgggcgag gctgcggttg tcgcgcagga gctcttcggt ttgcagcaag
     1681 tcgagcagtg ctttgcggct gcgttggtat tcttccttga tcatgccgaa gatgactttt
     1741 gctttgtcgg gtgattcgct caagccggca tagttttccg ccaaggtgat gtcggttttc
     1801 gccatcactt gttccatatt ggagagcatg gcttggaaga acgggttgct ttgggcgtgt
     1861 tcgcgcaggg cggcgagcgt ttcgggtttg tcttcgcaca aggtttccac cgcgctgccg
     1921 aaaccgtacc aagcaggcag catgaggcgg ttttgcatcc aggaaaatac ccacggaatc
     1981 gcgcgcaagt cttgaatccg cgctaaggtc ttgcggctgg cgggacggct gccgaggttg
     2041 agggtggcga tttcctgaat cgggctggtt tgcaggaagt agtcgatgaa gtcggggtgg
     2101 gtaatcagtt cgcggtagta tttgaacgat acgtccgaca atgcctgcat cagtttggca
     2161 tcagggtctt ttttatccgg caggatgctg gcttccaaag tcgcggcaac caaggtttcc
     2221 aagttgcgtt gggcattgcc ggggtcggcg tatttggcgg taatgacttc gccttgttcg
     2281 gtaatgcgga tttgtcccgc cacgctgccc gccggttggg cgagaatggc ttggtaagaa
     2341 gggccgccgc cgcgtccgac gctgccgccg cgaccgtgga acaggcgcat gcggacatcg
     2401 tattttttga agagttcgac caagcccaat tccgcctgat acagacacca tgagctggta
     2461 acatagccgc cgtctttgtt ggagtcggag tagccgagca taatttcttg gatgttgccg
     2521 cagctttcga gcagggcatc gtaccaatcg aggcggaaca tggtttccat gaccggacag
     2581 gcgttttcga gtgcttcgat ggtttcaaac agcggcacga tattgatgcg gctgcgcggt
     2641 ttgccgtttt ccaccgccag caggccgctt tctttcagca gcaatgccaa ggcgagcagg
     2701 tcgctgggtt gttcgcagtt ggaaataatg ctttgtgtaa cggcatcttc gccgaattcg
     2761 tctttgattt tgcgcgcttc gttgaaaatt gccagttcgt ggcgggtatg gtcgctgtat
     2821 gtgatgaacg ggctgtacag cggacgttga tggctcaatt cgcgcaacag ggcggcttgt
     2881 ttttgctctt cgttcaggct gttgtagtct tccaagcctg cgtgttggaa aagctcggca
     2941 accacatcgg cgtgtttgcc tgcgtgttgg cgcaggtcga gcggcatcat gtggaagccg
     3001 aacacggaca cggaacggat gaggtctgcc aaacggcctt cagcaagcag gcggctgccg
     3061 ttgtcgataa gggaacgttg caattttttc aaatcatcga gaaattcttg tgccgaagca
     3121 tacggctcga gaaagccgaa tttgcagccc atgcccaaac cgagcgcgcg cgctttgccc
     3181 atagcgcgtg ccatgatgta ggcgatggcg cggcggtagg gttcttcggt acgggcgatt
     3241 tcttcgtcag gcgagagggc ggccagtgcc attacgtcgt cgttgacttt gacgcggcgg
     3301 atggagagcg gcagttcgcg gtaaagtttg tcgagttcgc cacggtaaaa acggaacacg
     3361 gcatcggcgt ggcggcggaa ggcaaaacgc aaggtttcgg cagaaacgaa cggattgccg
     3421 tcgcggtcgc cgccaatcca gccgccgatt ttgaggatat tcggaacgca gacatcggga
     3481 tagaccatct gaaagtcgtg ttccatcttg cggtagagct tgggcagggc ttcgaaaaag
     3541 ctcatcggga agatggacac gccgttgttg atttcgtcgt tgacgctgag tttgtggcgg
     3601 cgcgtttcgc tggtctgcca caagcccagc aggatagtgt cgatttcgcg gcgcagccgt
     3661 gccagcgcgt cggcattggt gcagcgttcg cgttgcggca acagtgcgcg gatgcggcgg
     3721 ttgaagctta agacggtttg gcgttgcact tcggtcgggt gcgcggtcaa aacggcggta
     3781 acggacgtat tgtccaactg ccgctgcacc gatttgccgt cggctttccc cgctttgagc
     3841 ctgcggacgg tttccgtcag gctgccttcc gcgccgccgc gtccggcttc ttcgtggatt
     3901 tggcggcggc gttcgtggtg cacgtcttcg gcgatgttca aaatctgtgc gaacaggccg
     3961 caggccaagg ttaaatcgtg ggtttgttgt tcgtccaatt gcggcaatac tttttcaatc
     4021 aatgccgcgc tgtcgtcgga agtggacaag agtttgaccg tttcgacaac caatggcgag
     4081 gcttcttcgt gcaggaggtt gaacagggat tgtttcagaa attccgcgtc cgccgccaaa
     4141 gccgcgtcct ttggattgtt cagaatatgc agttgcatga tttttctctc attgccgtaa
     4201 atactgtaaa tgtacctcaa atgccgcatc cgtgccaaac cgttcacact ttaaccgccc
     4261 gtgtcccgaa atgccgtctg aagttgaacg ccgcccgacg gcagcgttac aatcgcccac
     4321 aactgttttt tccgaacatc atcatgacca cgaccgaaca cgacaacgac gatgcattcc
     4381 tgctgcggta cagccgccac atcctcttgg acgaaattgg catcgaaggg cagcagaaac
     4441 tttccgccgc gcatattttg gtcgtcggct gcggcggttt gggtgccgcc gccctgccct
     4501 atctcgccgc ttccggcatc ggcacgctga ccatagccga ttccgacacg gtcgagctgc
     4561 acaacctgca acgccaagtc gcatttgacg agggcgatgt cggcaagccc aaagccgaag
     4621 ccttggcaga ccgcctgaaa cgcatcaact ataacgtcga tgtccgcacc gtcaacgaaa
     4681 aactcgacgg ctgccgcctg accggtttgg ttcaaaccgc cgacatcgtt ttagactgct
     4741 gcgacaacta cgccacgcgc caagccgtca accgtgtctg cgtacaaacg aaaacaccgc
     4801 tggtttcagg agcagcggtg cgctttgaag ggcaacttgc cgtgtaccgt cccgacttgc
     4861 ccgactcgcc gtgttacgcc tgcctgtttg acggcggatc ggcttcagac ggcatctgtt
     4921 ccctcttcgg cgtgttctcg ccgctggtcg gcatcatcgg cagtacccaa gcggcggagg
     4981 ctctgaaaat cctgctggat gcgggcgaac cgtcgcacgg caggctggcg gtttaccgtg
     5041 ccttggaagg gggctggcaa tatttcgacc tgccgcgcaa ccccgaatgc ccgatttgcg
     5101 gcgcggaacg gtaaaaccct gccgccgttt cagacggtat ccaaacggat gcggcggaac
     5161 ggttttaaaa atttaaaaat ttacatttct ttgcaaaaaa aaaataaact taccttataa
     5221 ttgcagttgt tttagcaatg tctgtttcgc agactcattg agtaagacgt tttccccgta
     5281 atgtgtttgg ccgtctgtcc cctttgggtt cggacggctt tttttaggct gtgtttgaat
     5341 acccggttgg tttcgtctgt ttgcagggga aaaccgctta tttccgttcg ggcggaaaac
     5401 ggttccatcg gataaaaggc attttgtccg actgattaaa gttacatctt aggtttgcat
     5461 cggcaaaata ttcaaacaca gcctttttta aggaaatccg gatacggcgg cacatcaata
     5521 atgcggcgga atctcgtcgc gcagggaata cggctcttgc gtgtcgggat tcctgtcctg
     5581 cattttttga tacagcagcc tcaactgagc ctgctgcaaa tccagcgtct gccgcaattc
     5641 cgccaccatc gcgttcaggc cggcgattac gtcctcctga agcgcggatt ggatttccag
     5701 ctctgtgatg cggtgttcca actcttgaac cgcgtccatt tacagcacca tcgcggcaat
     5761 ccagccggca atcagcagcg ggatattgta gtggatgaag gtcgggataa cggaatcgcg
     5821 gatgtggtcg tgctgcccgt cggcgttcag ccccatcgtc gggcccagcg tggaatcgga
     5881 cgcaggcgaa ccggcatcgc ccaacgcccc cgccgtgccg acaatggcga cggtggcaag
     5941 cggcgaaaaa cccaaaccga cacacaaagg cacataaatc gcggcaataa tcggcaaagt
     6001 ggaaaaggac gaaccgatgc ccatcgttac caaaagcccc accaccagca tcgccaatgc
     6061 cgccatacct ttgctgttgc cgaatatcgc catactgctt tccaccagcg gctggatatg
     6121 cccggtcgca ttcatcacgg cggcaaaacc ctgcgcggca atcataatga agccgaccat
     6181 cgccatcatc ttgatacctt cgccgaatac gtcgtttgcc ttgtcgcggt tgatgacccc
     6241 caacatcata aatacggcga aaccgagcat cgcgcccaac accagcgagt cttcatacat
     6301 caactggatg gcaaaacata cggcaatggc ggcggcggcg gccaggctgc ggtaggcgga
     6361 cggctgcgga cggtttgccg catcggcgtt gcccgccgta tcggcattgt tgctttggta
     6421 caggcgcggt ttgcggtaat ggacaaacgc cagcaggagt ccggccagca ttcccaacgc
     6481 gggaatcgcc attgccgcca tcacgttaat gtttttcaca tcaagctgcg gcgcggcgga
     6541 atggatgttg cccaacagga tttcgttcaa aaaaatcgcg ccgaagccgt aaggcaggaa
     6601 catataagtc gtaaccagcc cgaaagtgat gacgcacgca atcaggcggc ggtcgatttt
     6661 caggcggttg aacaccaaaa gcagcggcgg aacaatcatc gggataaagg caatgtggat
     6721 ggggatgatg ttctgactca tcatgcccat cacaaggatg atggaaagca gcagccattt
     6781 gaccgcgccc tcgcccgaac gcacgctgtc gggcataccg ccccggttca gtttgcggac
     6841 gaccgcgccg gcaagctgct gcggcaggcc ggaatgggta atcgccatcg caaacgcacc
     6901 gagcatcgca taagaaagcg caatcttcgc gccgccttcc aaacctttgt tgaacacggg
     6961 gataatcccc gcctgactga cctgtcccgc cgcatcggca atgttttgca gcggcatacc
     7021 cgccaccgcg ccgccgacaa acgcgccgac cgtcaggctc aataccacgt gcacgcgcga
     7081 cagcgacagc accagcataa cgattacggc aactacgact gcattcattg tttatcgctc
     7141 caaacctata aatgtttaca tatcgaaaca catcataacc caataacgga aaacccgcca
     7201 attttgcaaa caattatttc aaatgcttca tatacttccc cagcgtaacc ctgtccaaac
     7261 ccgccaagtc cggcagggtt tccactcccg aaaaaccatt ctccgccaac acgccgcgca
     7321 ccgccgcgcc ctgatcgaaa ccgtgttcca gcaataaaaa accgccttcc gccaaacggt
     7381 cgggcgcgcc ttgcgccaag gtacggatgc agcttaggcc gtctgaaaag tcggtcagcg
     7441 cgatttgcgg ctcaaaccgc aaatcgcctt gcgacaaatg tttatcgccg ttttcgatat
     7501 agggcgggtt ggacacgatg atgtcccatt tcccttcaga cggcatatcg gtgtcgaacc
     7561 acgaaccgtg tgcaaattcg acccgcgcgc ccaaatccgc cgcatttttc cgcgccgttt
     7621 caagggcggg cgggctgatg tcggatgcgc gcacaaacgc atcggggcgt tcgagcgcga
     7681 cggttacggc aaccgcgccg ctgcccgtcc ccaaatccca cacgcgcccg ttttcgggca
     7741 ggcgcgccaa tacggcttcg accaagtgtt cggtttcggg gcgcggaatc agcacgctcg
     7801 gattgactgt aaagcgtctg ccataaaatt cgcgcacacc taaaatatag gcaaccggct
     7861 cgccgttcag acggcgttgc gccagcctgt ccgcccgctg tcggacttcg tccggcattt
     7921 cttccccgcc ccgcgtcaac aactgcacgc gcgtatattc cgaaacatat tgcagcagca
     7981 ttcttgcttc atttttaggc agttttgaca agcccaacca tttatcaaac gtcattttta
     8041 tcccgtctgc cgctgatgcg gcttttcttt ccttattctt tccggcaaac gtaccgatgg
     8101 tggcaaccgc aaatgcggca taccacaaat aaaatcctgc accgtagcgc acaatatccg
     8161 atgtattccc tgcttcatcg acgtatacgg ctttcacact gaaagccacc aacgccaagc
     8221 cccaaagtgc cgcatggaca ggcacgacct tcttccgcaa cgccagcaaa acaatggccg
     8281 ccaaccaaac ataattcgca tagaccgcac aatacctgat atccaaagaa gcaaatatcg
     8341 accccaaaat caaaacggtc aaaccctcca tgcttccatg attgcccaaa taaaatgcaa
     8401 cattggataa agacgctatc cacagggcaa ccgacaccag caacatcact atgggaaaac
     8461 ttgatttccg attctgttcc tgcatggttt tatcctaatg taaaaggccg cctgaaaacc
     8521 tttcagacgg catcgtgccg gattccgcgt cagattgcgc tgccgccgac ggtcagcccg
     8581 gcatcgatac gcagggtcgg ctgccccacg ccgacgggga cgctctgccc ttctttgccg
     8641 cacacgccga caccgctgtc caacgcggta tcgttgccta tcatcgaaac gtgtttcagc
     8701 acttcgggcc cgttgccgat aatggtcgcg cctttgacgg ggtattgcag cctgccgcct
     8761 tccacccacc atgcttcgga cgcgctgaac acaaatttgc cgctggtaat gtccacctgt
     8821 ccgccgccaa agttgacggc gtaaatgcct ttgtcgatgg acgcgatgat ttcttccggc
     8881 tcatagctgc cattttccat aaaggtattg gtcatgcgcg gcatcgggac ggaggcgtag
     8941 ctttcgcggc ggccgttgcc ggtggattgc gtacccgtca ggcgggcatt ggtttcgtcc
     9001 tgcatatagc cgactaaaat gccgtcttca atcaatacgg tgcggcgggt ttcgttgcct
     9061 tcgtcgtcga tgttgagcga accgcgccgg ccggcaatat cgccctgatc gacgacggta
     9121 acgcctttgg cggcgacgcg ctcgcctatt ctgccggaaa agacgctggt ccccttgcgg
     9181 ttgaaatcgc cttccaaacc gtgtccgacc gcttcgtgca gcaacacgcc cggccagccg
     9241 ttgcccaaaa cgacggtcat ttcgccagcg ggcgcggggc gggattcgag gttggtgagt
     9301 gcctgtttga cggcggcatc gacaaactgc cgcaccaagt tttcatcgaa ataagccaag
     9361 tcgtagcgtc cgccgccgcc cgcgctgccc tgttcgcggc gttcgccctg tttggcgata
     9421 acggtaacgt tcaggcgcac catcgggcgg atgtcggcgg cgtgtttgcc gtccagacgg
     9481 gcgaggtaaa ccatatcgta ttcgcaggtc aaaccggcca tcacttgcac gatgcgcgga
     9541 tcggcggctt tggcgattgc ttccactttg ttcaacagcg cgactttggc ggcggaatcg
     9601 aggccggcaa tggggtcgga cgcggaacaa accggcttgc cgcgcgtttc agacggcatt
     9661 ttggcggaca ccttgccgcc tgccgcccca atcgcgcgga cggcgcgggc ggaacggttt
     9721 atcgaatcga tgcacaggct gtcggcgtag gcaaaggcgg ttttatcgcc cgaaacggcg
     9781 cgcacgccca cgccctgatt gatttggaag ctgcccgatt tgacgatgcc ctcttccaaa
     9841 tgccagcttt cataagcggt gcgctggcag tagatgtcgg cataatcgac gtggtgcgcg
     9901 ccgatgatgc acaggctttt ggcgagcagt tcgggggaaa ggcggttggc ttcgagcagc
     9961 cgctctcgta cggcggaata ggtaggatgc atagtgtcgg cgcataaaaa atcaggggct
    10021 tgattatacg gcatttg
//
LOCUS       DAWHXZ010000056        10033 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000056 DAWHXZ010000000
VERSION     DAWHXZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10033
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.056"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(599..832)
                     /locus_tag="WIE35_000888"
     CDS             complement(599..832)
                     /locus_tag="WIE35_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ4153821.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(998..1198)
                     /locus_tag="WIE35_000889"
     CDS             complement(998..1198)
                     /locus_tag="WIE35_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ4153822.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1370..2245)
                     /gene="xerD"
                     /locus_tag="WIE35_000890"
     CDS             complement(1370..2245)
                     /gene="xerD"
                     /locus_tag="WIE35_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ4153823.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTCLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2297..2737)
                     /locus_tag="WIE35_000891"
     CDS             complement(2297..2737)
                     /locus_tag="WIE35_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ4153824.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            3058..3996
                     /locus_tag="WIE35_000892"
     CDS             3058..3996
                     /locus_tag="WIE35_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ4153825.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            4125..4418
                     /gene="hfq"
                     /locus_tag="WIE35_000893"
     CDS             4125..4418
                     /gene="hfq"
                     /locus_tag="WIE35_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ4153826.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            4503..5069
                     /locus_tag="WIE35_000894"
     CDS             4503..5069
                     /locus_tag="WIE35_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ4153827.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNNPPYLLAFEKLRQK"
     gene            5123..5497
                     /locus_tag="WIE35_000895"
     CDS             5123..5497
                     /locus_tag="WIE35_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ4153828.1"
                     /translation="MQYLIVKYSHQIFVTVTILLFNIRFFLLWKNPEKPLAGLWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSVKFYAV
                     YLLAICCVACIVYLAKTKVLPF"
     gene            5507..6001
                     /gene="folK"
                     /locus_tag="WIE35_000896"
     CDS             5507..6001
                     /gene="folK"
                     /locus_tag="WIE35_000896"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ4153829.1"
                     /translation="MNNRHFAVIALGSNLDNPAQQIRGALDALSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTDMDGISLLSLLNRIEADFGRERSFRNAPRTLDLDII
                     DFDGLSSDDPRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKQLGNQGIRL
                     LPDK"
     gene            complement(6128..6607)
                     /locus_tag="WIE35_000897"
     CDS             complement(6128..6607)
                     /locus_tag="WIE35_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ4153830.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYISLSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAMLQI"
     gene            complement(6827..7564)
                     /gene="ubiE"
                     /locus_tag="WIE35_000898"
     CDS             complement(6827..7564)
                     /gene="ubiE"
                     /locus_tag="WIE35_000898"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ4153831.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7607..7975)
                     /locus_tag="WIE35_000899"
     CDS             complement(7607..7975)
                     /locus_tag="WIE35_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ4153832.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            8095..9714
                     /gene="mnmC"
                     /locus_tag="WIE35_000900"
     CDS             8095..9714
                     /gene="mnmC"
                     /locus_tag="WIE35_000900"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ4153833.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITPAEAEKIAGIPLNTPYAEPLCGLFWQYGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFSATHIIYCTGAHSPR
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAIAAQILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(9711..9860)
                     /locus_tag="WIE35_000901"
     CDS             complement(9711..9860)
                     /locus_tag="WIE35_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153834.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT         2683 a         3050 c         2014 g         2286 t
ORIGIN      
        1 tttgctcgcc agcctactaa tgaaaaactg aaataacaag gggtggcaaa ttaccgcgcc
       61 cctttcgcca acctttcttc gagccatttt actacaatgg ttttaaaaat caaggattgc
      121 ctgatgccgc ccgcccctct gtttcctccg tcaagcaccg ccttggcggt tttcttatat
      181 agtggattaa caaaaaccag tactgcgttg cctcgccttg ccgtactatt tgtactgtct
      241 gcggcttcgt cgccttgtcc tgatttttgt taatccacta taaaatctac cgattacaca
      301 aacacatatc atctttacac aatcatgctt ccatcaacag taaaacatga tatgattgcc
      361 aacaataaca tctcacaata aattttctaa tttttattga aaaaatcaat aaataagaat
      421 tctcccaacc gacaaataaa ataaagaaag ggttaatatg caacatcgaa gaagactagc
      481 aatttaccaa gcatccaaac gtgcttcctt taccggcagg tcatcagccc cgcaaaaacg
      541 taaagaacgt taatttgaaa aaaatgccgt ctgaagtcct gcttcagacg gcattttttt
      601 accgttcgag aaactgtttc aacctgtcct catccaaaaa ccagtggcag atttcctcat
      661 ctcccgccaa caaaacggga accagctcat tgtatttttc ttccaaaaca ggattttcat
      721 ccacatcgac cacttccagc ccgaacccgt attcattttg aaaaggtttg agtgcatcgc
      781 gcattttgtg gcacaagctg caatattcac gaaacatcaa ggtcaatttc atccgcgttt
      841 ccttatctgt caatttgcac acgccaaagc cttagacgca gcagaatcat ggtctatttg
      901 ggaaaaaaca atgttttcaa ggaagatgat actcaagtcc tgccaaaaac agcaaaaatg
      961 ccgtctgaac agttcagacg gcatttccaa aaccgtttta cgcttgaaca ttgatacccg
     1021 ccgtaaccgt cggttgcgca ttgtgcgccg tcagaaacga agcggcaagc tccccacagc
     1081 agccgcagca gctcgctacg cccaattgcg actgcaaatc gcccattgtg gtcgcgccgg
     1141 cggcgatggt ttccttgatt tgatggtcgg taacggcatt gcagatgcag acaaacattt
     1201 tgtgctccgt gtgttctcaa actatcgtac cgatagcggc ttttattatc gtatgcgaat
     1261 ataaataaaa acggttcgca ttgcaaggtc ggtatacacg gtttgtcttg gtaatttttt
     1321 atcaagtttg cattttcgat tgatttctta ttaaaccaaa gtagaagcct cagtttcggg
     1381 aatggtgttc cttcactaca ccctgcaacc atacgttggc aacatgggta taaatctgcg
     1441 tcgtattcaa atcggcatgt cccaacatat cctgaaccac gcgcaaatcc aagccgtgcc
     1501 gcaccagatg cgtggcaaag gcgtggcgca ggctgtgcgg gctgatgtgc ccgatgcctg
     1561 cctgacttgc atattctttg acaatcatcc atgccaactg acgggaaatg cccgtctttt
     1621 tctgactgac aaacaatgcg tcgcaattcc tgcctttcag cagaagtggg cgtgcctccg
     1681 tataatagcg ttccacccaa tacgccgact cctgccccat cgggaccatc ctctgcttat
     1741 caccctttcc cagcgcggta atacagcccc tgtccaaatc cacattgccg aagttcagcc
     1801 cgaccgcctc gctgacgcgc aagccggtcg cgtacatcaa ttcgagcaaa gccttatccc
     1861 gcaaaccgtg cggcgtgtcg gtatccgggg cggcaagcag tcgggaaatc tgctgctcgg
     1921 tgatcagggt cggaatattc ttgtcgattt tgggcggttt cagcaggcag gtgggattgt
     1981 ccgtccttat gccttcacgc tccatccata tatacaggcg tttgcacgcc gataatgcgc
     2041 gcgcctgcga actccgttgc tctccgtcaa cataaaccgc cgccgccaaa tccgcttcgt
     2101 ccgcatcctt cagcgttctg cccgattggg acaggcggcg ggcgattttt tccaaatcgc
     2161 gccggtaacc gtttaaagta ttctgactga gccgcctgtc caaccacagc gtttcaagca
     2221 gcctgtcgat caaaccttct tccataccgt tccaaacaaa tgccgtctga atcttcttca
     2281 gacggcatgg ttcacattat cgggaaagcg tttccaatac ttcctgcgcg tgacccgcca
     2341 ctttgacttt ccgccattca tgggcgattt ctccatcctt attcaagacg aacgtactgc
     2401 gctcgatacc taacgactct ttcccgtaca gtttcttcaa tttgatgaca tcaaacaggc
     2461 ggcacactgt ttcatccttg tcgctcaaca gctcgaaccg gaaaccctgc ttggcgcaaa
     2521 aattctgatg cgcctttacg ccgtcgcggg aaataccgac cacggtataa cccaatgcct
     2581 caaactgttc caaacgcgca ttgaaatcca agccttccgt cgtacagcca ggcgtactgt
     2641 ctttcggata aaaatacacg accaaaggca gatgttctgc cgaatgaaaa tccgcaccgc
     2701 tgctcgaagg cagggtaaat tcatatttca catccatagt cctactcccg atattcccat
     2761 tattcaaaac ggcacgcaga cgcaccgccg caattgccaa accaaccccg attctaccgc
     2821 cccaaaggac aaggattcaa ccgccggaaa catccaaacc gacacacgac ggcatgaaaa
     2881 atatccatgt caaaccacaa aatatgttcc gatttaaaaa cagaatgtta taaaacccaa
     2941 tccccaaaac acaacaagac cgcccgccta cgggcagtct cctgtcagac gacatacttt
     3001 acagatggct gttttttcaa caaaataacg ccaatactca aaaatatgga atcaaaaatg
     3061 tccatccata ctctgaaacg cctgccctca tcgctgctgc tcggtctctg cctttccctg
     3121 ccgtcagccc acctttttgc cgacaacgac attttagggc aatttttaga acagaacatg
     3181 cttacctcct ccgatccgat agaaatattc gccgaaagca caatacaccc caccaacacc
     3241 caagccatta caggcggtct gattctctcc tcacagtctg ccctggtcgt caacaacaaa
     3301 accggacaga tactgtatca gaaaaacgcc gacaggatta tgcccatcgc ctccatttcc
     3361 aaactgatga gcgcgatggt cgttttggat gcaaacttgg acatgaacga aaccgttacc
     3421 attacgcccg acgaaatcga ccgcatcaaa gggaccggca gccgtcttgc cataggtacg
     3481 gcacttacac gcaaagaact gctgcacctg agcctgatga gcagcgaaaa ccgcgccacc
     3541 catgcattgg gcagaaccta ccccggcggc atgggcgcat ttgtcgccgc catgaaccgc
     3601 aaagcccaaa gcctcggtat gtacggcagc cgcttttacg aaccgaccgg actcaacttc
     3661 caaaacgttt ccaccgccaa agacctgagc cttatggtca acgccgccgc ccaatatccg
     3721 caaatccgca ccaactcgac ttccaactac gcctcggtac agaccaaaaa cgggcagcag
     3781 aactacaaaa actccaatgc cctggtcaga gaaggcatgt ggaacatcga attgcagaaa
     3841 accggctaca tacgcgaagc aggcaggtct atggttgtca aagccaacat tcaaaatcaa
     3901 cccgttacca tcgtattgct gaactcgccc acatccgcca cacgcgtcaa cgacgcccgc
     3961 aaaatcgaat cgtggatgct gcagcaacgc tcctgacata caaatgcccg gcggaaaacc
     4021 gcccaaacac aaaaaaaatt ggtaattttt cctattcaag gctacaattc gacacgcaca
     4081 gggcgtctgc ccgatttcac aatcataata acggagttaa aaatatgaca gctaaaggac
     4141 aaatgttgca agatcccttc ctgaacgccc tgcgtaaaga gcacgttccg gtttcgattt
     4201 acttagttaa cggtatcaaa ttacaaggtc aggttgagtc tttcgatcaa tacgttgttc
     4261 tcctgagaaa cacttccgtc acccaaatgg tttacaaaca cgccatttcc accatcgtac
     4321 cggcacgctc cgtcaaccta caacatgaaa acagacccca agccgcaccg acttcgaccc
     4381 tcgtccaagt ggaaaccgtc cagcagcctg ccgaataatc cgcacgaagc atgacgtgtc
     4441 atatctttca ataccttacc ggacaacggt aaggtatttt tattttcaga cagcatttaa
     4501 aaatgttatt gcaaaacatc cttccattcg cccattgcct tttgcggaag gcacttcccg
     4561 aaggtggcaa tgctttggac ggcaccgccg gcaacggaca cgacaccctt ttcctcgcac
     4621 aaaccgcagg catccggggg aaagtgtggg cattcgacat ccagccgcaa gccctgagcc
     4681 gcactcgatg ccgtctgaaa gaagcgagat acgagaatgt gcaactcatc ttagacggac
     4741 acgaaaacct gaaacaatat gtttcagaac cactaaatgc cgccattttc aactttggct
     4801 ggctgcccgg cggagataaa agccttacca cccatacgga aaccagcatt cccgcccttt
     4861 gcgcaaccct ctccctgctg aaagaaggcg gtatgcttat tgccgtcctc tatccgggac
     4921 acgaaaacgg caaacaggag gcagaagcaa tcgaacaatg ggcaaaaaac ctgcctcaag
     4981 aacagtttgc cgttttgcgt tacggcttta ccaaccggaa aaacaacccg ccctatctgt
     5041 tggcatttga aaagttacgt caaaaataaa tgtttgcggt aaaataagcc gattccaacc
     5101 cttattttga taagagccga ctatgcagta tctgattgtc aaatacagcc accaaatctt
     5161 cgttaccgtt accattttgt tgttcaacat ccgctttttc ctgctttgga aaaatcccga
     5221 aaaacctttg gcgggcttgt ggaaggcact gccccacctc aacgacacga tgctgctgtt
     5281 tacgggattg tggctgatga aaatcaccca tttctccccg ttcaacgcgc cttggctcgg
     5341 cacaaaaatc ctgcttctgc tcgcctatat cgcattgggt atgatgatga tgcgcgcccg
     5401 cccccgctcg gtcaaattct atgccgttta cctgcttgcc atttgttgcg tcgcctgtat
     5461 cgtttacctt gccaaaacca aagtcctgcc attctgaaac actgccatga acaacagaca
     5521 ttttgccgtc atcgccttgg gcagcaacct tgacaacccc gcacaacaaa tacgcggcgc
     5581 attggacgcg ctctcgtccc accccgacat ccggcttgaa caggcttcct cactgtatat
     5641 gaccgcgccc gtcggttacg acaatcagcc cgatttcgtc aatgccgtct gcaccgtctc
     5701 caccgatatg gacggcatct cgctgctctc tttgctcaac cgtatcgagg cggatttcgg
     5761 gcgcgaacgc agtttccgaa acgcgccgcg cacattggat ttggacatca tcgactttga
     5821 cggcctatcc agcgacgacc cccgcctcac cctgccgcat ccgcgcgcac acgaacgcag
     5881 tttcgtcata cgccctttgg cagaaatcct ccctgatttt gttttaggaa aacacggaaa
     5941 agttgccgaa ttgtcaaaac aactgggcaa tcaaggtatc cgtcttttac cggataagta
     6001 attccgcacg cggatgccgt ctgaatgccg gtttcagacg gcatttttcc tttgccgcca
     6061 acacgcgtgc aaaaaaatcg ccccttggaa aaggggcgca aaaggaacac aaaccactac
     6121 caaaacttta aatctgcaac attgcctgcc gcatactgta tccgacagga tataaagccc
     6181 tcactaaatc gtttcgagaa atccaaactt cttcatcgcc gacagaaaat ctgcctttct
     6241 ccggcaccag ctccaacaaa aacggttgaa ccgccgtatg cagcctgtcc ttacaccgcc
     6301 ccagctttct ggacaaggcg gacagggcac gcttatagac actatccttg cggtcaagtt
     6361 cccgcgcact gccgacccag ctcaaacgaa ggttgcggct ttcatcctca atcagcaaaa
     6421 tgcctgtttt gacttccccc acctctggct cgcatgaaag cgaaatataa tatttgccgt
     6481 cgatgttgac atacgccgcc tcatgcacct gacagttgca gccggaagtg gcaagcagcg
     6541 aataaacaga atcccgatgc agtttcaaaa tatcaaggga aatctgtttg aaaatacttt
     6601 ccggcatttt tccctccttt cgtctgctgc gttgtttact ggattgactg cattatacag
     6661 acatctccca atcttgcaaa tgataattgt tcctattttt taaaaaattt ataaattatt
     6721 gatagatatg tgaattaatt ttaagattta aaatctcttt ctcaaaaaaa cacaaaaatg
     6781 ccgtctgaaa cgtgttcaga cggcattggc tgcacaggca atccgtttaa aatttcacgc
     6841 ccttatgcag cgcaacgatc cccgcactca tattatgata gtccacactg tcaaatccgg
     6901 catccagcat catttgcttt aaagtttcct gatcggggtg catacggatg gattcggcga
     6961 gatactggta gctgtccgca tctttggcaa tcagtttgcc catgaccggc agcagcttga
     7021 acgaatagaa atcatacgcg ccttcgagcg gcttgtagat tttggaaaac tccaacacca
     7081 gcaacgtgcc gccaggtttc aaaacacgat acatctcttt cagcgcggca tctttatgcg
     7141 tcatgttccg caagccgaac gccacggaaa ctaagttgaa ataattgtca gggaaaggca
     7201 gtttttccgc atcggcaagc gacacgggca aaatcatgcc ttcgttcaac agacggtcgc
     7261 gcccgacggt cagcatagag gaattaatat cggtcagcca aacctcgcct tccttgccga
     7321 cccgtttcgc ccaaccgcgc gacaaatcgc ccgtaccgcc cgcaatgtcc aacactttat
     7381 cgcctttttt caggtgcgcc gtgttgatgg tgaaatgctt ccacaccctg tgcagccctg
     7441 ccgacatgac atcattcata atgtcgtagt ttttggcgac ggagtggaac acttccgcca
     7501 ctttgccggc tttttcatct tcgttgaccg tgctaaaccc gaaatgcgtt ttttgtccgc
     7561 ccatattcta accacctccc tgataccgcc ctacggcatc ccaaacttaa caatctgatt
     7621 gcttttcaaa acgcgacgcg cccgccgccg ccaatttgtc caaatattcc tgccacatcg
     7681 catcgtatcc gtatgccagt ctgtgcagat acgcccaatc gtaaagaccg ctgtcgtgcc
     7741 cgtccgaaaa actgattttc agcgcgtact gtccgacagg ctgcaaatcc gtaatttgca
     7801 catctgcctt accggtctgc aaaacatcct gtcccacacc gtgtccgcgc acttccgcac
     7861 tcggcgaata gacgcgtaaa aattcggcag gcagattttt aggttcgtcc ccataagtca
     7921 aaaccaacac ggtacggttt ttcaacaaac ggatttcaac aggaatatta ttcaaaaaac
     7981 acctcggttc ggcatacaat atccggcagg cgcaggcgtg ccttcaaacg gcgcgtattt
     8041 taacagcttt atcccgatac cgatattcaa acccacactt taaacggcat tgatatggac
     8101 aatctcgtat gggacggcgt tcccgacatc cgcacactcg accaaaccat ccgcaaacac
     8161 gcacacccgc tcaacctgat tgtctgcctc cccgataatc agattcccga ttttcaaacc
     8221 gcacaagatg cttcggacgc ggaatgccgt ctgaagcacc gtttggatca ggcaacgcag
     8281 tgcctccagt tcgacagcat caacctcatc gaacacatcc tgcccgatgt ccgcttctgg
     8341 ctggttcccc cttcacgcac ccgccgcctg cacgaacact tccaccacat ttcctggcag
     8401 accgaagcca tcccgcaaac cgaaagcaaa cccgacaaac cctggtttgc acttccacaa
     8461 acatccgaac ggcaaaaacc ggaacacatc ctcgttatcg gcgcgggcat atccggcgcg
     8521 gcaaccgccc acgccttagc atcacacggc atttccgtta ccgtattgga agcccgaaaa
     8581 gccgcccaag ccgccagcgg caaccgccaa gggctgctct acgccaaaat ctcgccgcac
     8641 gacaccgaac aaaccgaact gctgcttgcc ggctacggct acaccaaacg cctgctcgga
     8701 cacatcctgc ccgaatccga aacctggggc ggcaacggca tcatccacct caattacagc
     8761 cgcaccgaac aacaacgcaa tcacgaattg ggtttgcaaa aacaccataa ccacctctac
     8821 cgcagcatca cgccggcaga agccgaaaaa atcgccggca tcccgctgaa cacgccctac
     8881 gccgaaccat tatgcggact gttttggcag tacggcgtat ggctcaatcc acccgcattc
     8941 gtccgcaccc tcctcaacca tccgctcatt ggactacacg aagacacacc gttaaccgac
     9001 atttcccacg acggggaaaa gtggattgca agcacgccaa acggcatatt ttccgccaca
     9061 cacatcatct actgcaccgg cgcgcacagc cctcgcctgc ccgaaaccaa cctcgccgcc
     9121 ctgcctctca ggcaaatacg cggacaaacc ggcctcacac cgtccacccc gttttccgaa
     9181 caactgcgtt gcgccgtttc aggcgaaagc tacatcagcc cgtcgtggca cggactgcac
     9241 tgctacggcg cgagttttat tcccaacagc agccataccg gatggaacga agccgaagaa
     9301 gcctcaaacc gccaagcatt ggcacacctt aaccccgccc tttccgaatc attgtttgcc
     9361 gccaacccaa acccccaaaa acaccaaggg cacgccgcca tacgctgcga cagccccgac
     9421 caccttcccc tagtcggcgc actcggcgac attgccgcca tgcggcagac ctacaccaaa
     9481 ctcgcgctgg acaaaaacta ccgcatcgac accccatgcc catacctgcc taatgcctac
     9541 gtcaacaccg cgcacggcac ccgcggactc gccaccgccc ccatctgcgc cgccgccatt
     9601 gcagcccaaa tcctaggctt gccccatccc ttttcacaac gcctgcgcca cgccctacac
     9661 cccaaccgca ccatcatccg cgccatcgtc agaaggaagg atctaacccc ttaagccttt
     9721 gccgcatgcc gtcccgacca cagaaaatca tattcaaata aacataccaa tcctgacggt
     9781 aaaatatcga aaacacccat aacgttttct tcccaaaata aggagtaagg aatgcctaac
     9841 aaattgcctt tgctgtccaa gatgaatgtt ccgtaacatt cgaagacttc cttaaccctg
     9901 ccagccgttt cggtttctgt accccctgtt agataaggaa ggaatgaaaa agccctgttg
     9961 attgtatgaa gtaaagggcg ttaatttttg ttaatcttcc cttctcagga actcaatata
    10021 taaggatgtt aat
//
LOCUS       DAWHXZ010000057         9891 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000057 DAWHXZ010000000
VERSION     DAWHXZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9891
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.057"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..224
                     /locus_tag="WIE35_000902"
     CDS             <1..224
                     /locus_tag="WIE35_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ4153835.1"
                     /translation="MKSSGVNKEIQGKKLSLWAKRQDGSVKWFCGQPVQRANANGAND
                     DVTADNDNSNNGINTKHLPSTCRDAASAS"
     gene            963..1886
                     /locus_tag="WIE35_000903"
     CDS             963..1886
                     /locus_tag="WIE35_000903"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ4153836.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
     gene            2826..4274
                     /gene="gnd"
                     /locus_tag="WIE35_000904"
     CDS             2826..4274
                     /gene="gnd"
                     /locus_tag="WIE35_000904"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ4153837.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDERAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPTWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            4337..5608
                     /gene="waaA"
                     /locus_tag="WIE35_000905"
     CDS             4337..5608
                     /gene="waaA"
                     /locus_tag="WIE35_000905"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ4153838.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMTECRKSGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGDRQVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACWHALASGAAVQVESADAWREAVEKTLSSKGGGMQMQ
                     VRVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            5649..6098
                     /locus_tag="WIE35_000906"
     CDS             5649..6098
                     /locus_tag="WIE35_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ4153839.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGLVSCEARRE
                     VENAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            6131..7105
                     /locus_tag="WIE35_000907"
     CDS             6131..7105
                     /locus_tag="WIE35_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ4153840.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGIKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEGDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLSDVEERFIFLKYGLA
                     FVLSFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            7174..7249
                     /locus_tag="WIE35_000908"
     tRNA            7174..7249
                     /locus_tag="WIE35_000908"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7207..7209,aa:Lys,seq:ttt)
     gene            7469..8722
                     /gene="murA"
                     /locus_tag="WIE35_000909"
     CDS             7469..8722
                     /gene="murA"
                     /locus_tag="WIE35_000909"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ4153841.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGA
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            8803..>9891
                     /locus_tag="WIE35_000910"
     CDS             8803..>9891
                     /locus_tag="WIE35_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ4153842.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMIHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDDMILDIGPKSAAELAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIG"
BASE COUNT         2359 a         2482 c         2727 g         2323 t
ORIGIN      
        1 aaatgaaatc aagcggcgta aacaaagaaa tccaaggcaa aaaactctcc ctgtgggcca
       61 agcgtcaaga cggttcggta aaatggttct gcggacagcc ggttcagcgc gccaacgcca
      121 acggcgccaa cgacgacgtt accgccgaca acgacaacag caacaacgga atcaacacca
      181 aacacctgcc gtcaacctgc cgcgatgcgg catctgccag ctaaggtaaa ttataccata
      241 aattttaaat aaatcaagcg gtaagtgatt ttccacggcc actcggatta atccgggtgg
      301 cttccttttt aaaggtttgc aaggcaagcg gggtcgtccg ttttggtgga aaataatata
      361 tcgactgcgc ttcaaggctc tgcgcgtacc tcattgccgc ccgtttaaac acgtggttta
      421 tctgacaggc gcgcaatccg ccctctcatt taccgaacaa gcggactaga ttcccgccta
      481 ctcgggaatg atgaagctat ccgcacggaa acctgcacca cgtcattccc acaaggacag
      541 aaaaccaaaa acagaaacct aaaattcgtc attcccgcga aagtgggaat ccagaacgca
      601 aaatctcaag aaaccgtttt cccgataagt ttccgtgccg acgaggctgg attcccgcct
      661 gcgcgggaat gacgaatttt aggttgctgt ttttggtttt ctgttttggt tttatgtcct
      721 tgtgggaatg acggtttacg cgaaaatgcc gtccgaaagg ttttcggacg gcattttttg
      781 cgtttttcag gaggggggcg gcttcgtcgc cttgtcttga tttttgttaa tccactatac
      841 atttttatgc ggcttacgta ttgaaaacgg atacattggc aaatactatg gaaaaacata
      901 gcttgttttt ccggttttat cgtattattc caaaacttac cccattgata aggaagcggt
      961 ttatgctgca aagaactttg gcgaaatcca tcagcgttac cggagtcggg ctgcattcgg
     1021 gcgaacgcgt cgcgctgacc ctgcaccccg cgcctgaaaa cagcgggatt tccttccgcc
     1081 gtaccgattt ggacggcgag atgggcgaac aaatcaagct gaccccttat ttgatcaacg
     1141 atacccgcct ttcctccacc atcgttaccg acaaaggcgt gcgcgtcggc acgatcgaac
     1201 acattatgtc cgcgctgtcc gcctacggta tcgacaacgc gctgattgag ctgaacgcgc
     1261 ccgaaatccc gattatggac ggctccagcc tgccgtttat ttaccttttg caagatgcgg
     1321 gcgtggtcga tcaaaaggcg caaaagcgtt ttttgaaaat cctcaagcct gtcgaaatca
     1381 aagaagcggg caaatgggtg cgctttacgc cgtatgacgg ctttaaggta acgctgacca
     1441 tcgaattcga ccatccggtt ttcaaccgca gctcgcccac ttttgaaatt gatttcgccg
     1501 gcaaatccta catcgacgaa atcgcgcgcg cgcgcacttt cggctttatg cacgaagtgg
     1561 aaatgatgcg cgcccacaat ctgggtttgg gcggcaattt gaacaacgct atcgtgattg
     1621 acgacacgga tgtcctgaat cctgaaggct tgcgctatcc cgatgagttt gtgcgccaca
     1681 aaatccttga tgccatcggt gatttgtata tcgtcggaca tccgattgtc ggcgcgtttg
     1741 aaggctacaa atcgggacac gccatcaaca acgcactctt gcgcgcggtt ttggcagacg
     1801 aaacggctta cgaccgggtg gaatttgccg acagcgatga tttgcccgac gcatttcacg
     1861 agctgaacat cagaacttgt ggataacgct ttctattttt tccggtaaat tcctaaaact
     1921 taaaatttca tcattcctac aaggacaaaa aaccaaaaca gaaacctaaa attcgtcatt
     1981 cccgcgaacc tacatcccgt cattcccacg aaagtgggaa tccagaacgc aaaatctaaa
     2041 gaaaccgctt tcccgataag tttccgtacc gacagaccta gattcccgcc tgcgcgggaa
     2101 tgacggcgga gcggtttctg ttttttccga taaattccta aaactcaaaa tttcatcatt
     2161 cccactttcg tgggaatgac gggatgtaag ttcgtgggaa tgacgcggta caggtttccg
     2221 ttacgtatcc ccatccgaaa gtttacttat acacaagcaa tccaaagtta tctgactgaa
     2281 atattgtgag atttacccgc aaatctcatt ttctccacca cttatacaca aactcggatg
     2341 cacactctgc caatatgcag agaaaaagac attcgtgccg ttaacttact attcccacac
     2401 gttccaaaca catttccctc tcaaacctcc acagtattcc cgttcttaaa ggcgccagca
     2461 gccattcggt aaagaaaagt aggatgccat ctgcacaaca agcaaactgt ccacaaaaac
     2521 tattcttcat gaaaaacaat ccacaggtat gcacaaaagg gaatccgcct taccgacacc
     2581 cttttaaaac ggcaatctcg atgattttta aaataaaaaa aagttaccca cagaaaatgt
     2641 ctcctatcaa tataatcatc gttttattat ttaaatttta tattttattt ataagccttc
     2701 agggaaacgc acggtaaagc gtaaaggcat cttttcaaaa aattaaaaag ttaaggattg
     2761 ttaaacaagt gtaaatcagg tagagttgcg gtcgataaat taaatctata acggagcttc
     2821 ctgcaatgaa aggcgatatt ggtgtaatcg gtttggcggt tatggggcaa aacctgattt
     2881 tgaatatgaa cgattgcgga tttaaggttg tcgcctacaa ccgaacaatc ggtaaggtgg
     2941 acgaattttt aaacggcgcg gctaagggaa cggatattgt cggcgcgtat tccctgcaag
     3001 acttggtcga taagctggca aaaccgagaa aaatcatgat gatggttcgc gcaggttcgg
     3061 tggttgacga ctttatcgaa caactgcttc cgttgttgga agaaggcgac atcattatcg
     3121 acggcggcaa tgccaattac cccgacacga cacggcggac acattacctt gccgaaaaag
     3181 ggattttgtt tgtcggcgcg ggcgtatccg gcggcgaaga aggcgcgcgg cacggcccgt
     3241 ctatcatgcc gggcggagat gagcgcgctt gggaagccgt caaaccgatt tttcaggcga
     3301 ttgccgccaa aacgccgcag ggcgaaccgt gttgcgactg ggtcggcagg gacggcgcgg
     3361 ggcattttgt caaaatggtg cataacggca tcgaatacgg cgatatgcag ttgatttgcg
     3421 aagcgtacca gttcatgaaa gacggcttgg ggctgtctta cgacgaaatg caccgcgtgt
     3481 ttgccgagtg gaacaaaacc gagctggatt cttatctgat tgaaatcaca gcggcaattt
     3541 tgggctataa ggacgaaggc ggcgaaccgc tggtcgagaa aatcctcgat acggcagggc
     3601 aaaaaggcac gggcaaatgg acgggcatca atgctttgga tttgggtatt ccgttgacgc
     3661 tgatttccga agcggtattc gcccgctgcg tttcgtcgtt caaagaacag cgcgtgcaga
     3721 ccggcaagct attcgcccga accgttacgc ccgtcgaagg cggcaaacaa gaatgggttg
     3781 aggcgttgag acaggccctg cttgcatcta aaatcatttc ctacgcacaa ggcttcatgc
     3841 tgatccgcga agcgggcgaa agctacggct gggatttgga ctacggcaac actgcgctgc
     3901 tgtggcgcga ggggtgcatc atccgcagcg cattcttaag caatatccgc gacgcgtatg
     3961 aaaacaatcc cgatttggtg ttcttgggtg cggatccgta tttcaaaaat attttggaaa
     4021 actgcctgcc gacgtggcgc aaggtggttg ccaaggcggt cgaatgcggc atccctatgc
     4081 cttgcatggc ttcggcgatt acgttcttgg acggctacac gaccgaacgt ctgcctgcca
     4141 acctcttgca ggcacagcgc gactacttcg gcgcgcacac ttacgaacgc accgacaaac
     4201 cgcgcggcga gtttttccat accaactgga cgggcaaggg cggagatacc gcttcgacaa
     4261 cctacgatat ttgattccgt tgccgaaatg ccgtccgaac cgttcggacg gcatttgttt
     4321 tgcaggaaat agaaagatgt tccaatggct ttatgatgta ttgtggctgc ttgcgccgat
     4381 atggatacgg cgttatttgg acaaacgctc cggaagtgcc ccggcatatc gggcgcatcg
     4441 ggacgagcgt ttcggcaagc cgtatccgaa tcccgttacc ggcgcggttt ggatacacgc
     4501 cgtttcggtc ggagaaacgc gtgccgccca gcccttgata cgcgagttgc ggcagcgttt
     4561 tcccgatgcg ccgctgctga tgacgcagat gaccccgacg ggacgggaaa ccgcgcaagt
     4621 tctgtttcct gatgcacaat gccgttatct tccgtatgac aaaaaaacgt gggtacggca
     4681 gtttttgcgc gaacaccgcc cgatgttcgg cattttgatg gaaaccgaaa tctggcccaa
     4741 cctgatgacg gagtgccgga aatcgggcgt tccgctgttt ttggcgaatg cgcggctgtc
     4801 ggaaaaatcg ctaaacggtt atctgaaagt ccgccgcctg atccgtcctg ccgccgcttc
     4861 gctgacgggt tgtctggcgc agacggaggc ggatgcggcg cggttggcga aattgggcgc
     4921 ggcatccgtg caggtgtgcg gcaataccaa atacgacctg atgccgtcgg aacagatgaa
     4981 aacgctggcg gggcagtttg aaaaacgcat cggagaccgg caggttgccg tgtgcggcag
     5041 cacgcgcgtt tatcggggtg aagacgaggc ggaaaaactg ctggcggcgt ggcaacaata
     5101 tcgcggcgat gcgctgctgg tcgtcgtgcc gcgccatccc gagcattttc agacggtatt
     5161 tgaaacggca aaacgcttcg ggtttaaggt tcagcggcgc agcgacggtt tgccggtcga
     5221 acctgatacg caggtgtgga taggcgacag tatgggcgag ctgtatgcgt attacttgtg
     5281 cgccgatgtc gcttttgtcg gcggcagtct ggtcgattcg ggctgtcaga acattatcga
     5341 accgctttcc tgcggcgttc cgacgatatt cggcttttca acctacaatt tttccgaagc
     5401 ctgctggcac gccttggcat cgggggcggc ggttcaagtc gaatcggcgg atgcgtggcg
     5461 tgaagccgtt gaaaaaacct tatcgtccaa ggggggcgga atgcagatgc aggtgcgcgt
     5521 ggacggcttt atcgcacaac atcgcggagc gggcgcgaga atcgccgagg cggtgcggga
     5581 agcggtatgc ggacatcggg ggcgatagtg atacaatccg tatccgagtt ttccgtttgg
     5641 agcatcgtat gagtatttat gccgtcgcgc acatcgttca tctgtattgc gctattgcct
     5701 ttgtcggcgg cgtgtttttt gaagtgctgg ttttgtccgt cctgcatacg ggactggtgt
     5761 cgtgcgaggc gcggcgcgaa gtggaaaatg caatgtctta ccgcgccgtc agggtgatgc
     5821 cgtttgtggt cggactgctg ttcgccagcg gcatcgtgat ggcggcaaac cgctatcttt
     5881 ctatattggg cgaaccgttt gccacttcct tcggtacgat gctgacgctg aaaatcctgt
     5941 tggcgttcag cgtgttggcg cacttcgcca tcgccgtcgt caaaatggcg cgttccacac
     6001 tgaccgtcgg ctggtcgaaa tacatacaca ccgtcgtctt tacccatatg ctgctgattg
     6061 tctttttggc aaaagcgatg ttttatatca gctggtaaca caattacatt tcaaatcagg
     6121 agattgaacc atgacagaat atcccggcat cgggtcgccg ttgttttacg gcgttttttt
     6181 tgcggcagtg ctggtcatga ttgccttgga tatgttttcg ctgaagaaaa acggcagcca
     6241 taaagtcggc atcaaagaag ccttggcatg gagcggcttg tgggttgccg tatcctgcct
     6301 gttcgcgggc tggctgtatt tcgaactcgc cggcaatccc ggctacggcg cggcagtcgc
     6361 aaaagaaaaa gtattggaat tctttaccgg ctacattttg gaaaaatcgt tggcagtcga
     6421 taatattttc gtgttcctga tgatattcgg ctacttcaaa gtcgcaccgc agtttcagca
     6481 ccgcgtgctg ctgtacggcg tattgggcgc attggtattg cgcaccgtca tgattttcgt
     6541 cggcgcggca ctggttcggc agttcgagtg gattctgtat ctgttcggcg cgttcctgct
     6601 ctataccggc atacacatga tgaagcccga aggcgatgag gaaggggatt tggcaaacag
     6661 caggctgctg aatgccgtca agaaagtcgt tccggtcggc acggaatttc acggcgagaa
     6721 attttttacc gtcgaaaacg gcaaaaaaat cgccacgccg ctgtttttgg tgctggtcat
     6781 gattgaattg agcgatgtcg tgtttgccgt ggacagcatc cccgccgtct ttgccgttac
     6841 caccgatccg tttatcgtgc tgacctccaa tattttcgcc attttgggtt tgcgggcgat
     6901 gtatttcctg ctgtcggatg tggaggaacg ctttatcttc ctgaaatacg gcttggcatt
     6961 cgtgttgagt tttatcggtg tgaaaatgct ggtaatgcat tgggtgcata tcccgatttc
     7021 cgtttcgctg tcggtcgtgt tcggcgcgtt gggtgcatcg atactgacct cgttaattta
     7081 tacgaaaaaa cagcctgata aataaaatgc cgtctgaacg ccgacaaaat ggttgacagc
     7141 atcaggcttg atgtatagaa tttcgagctt atcgggtcgt tagctcagtc ggtagagcag
     7201 cggactttta atccgttggt cgagcgttcg aatcgctcac gacccaccag ataaaacaaa
     7261 aaaagctgag ttttcaaaac tcggcttttt ttgtcttccg acaatccttg ttcggaaagc
     7321 gcggcatttc gtcaaattct ttggtgcggc ttccgtattt ttcccgaaaa tcccttataa
     7381 ttccgcctct gcaaccttca tcgggtgatg ctttatgcgt tgtcccgagt attgaaatat
     7441 tacaagtacc caccaggaaa gaacaaacgt ggacaaactg aaaatctccg caaacggccc
     7501 gctcaacggg gaaataacgg tctcgggcgc gaaaaacgcg gcattgccgc tgatgtgcgc
     7561 gggtttgctg acatcgggta cgttgcgcct gaaaaatgtg cctatgctgg cagatgtgaa
     7621 aaccacacaa aagctgcttc aggggatggg cgcgcgcgtc ctgaccgaca atatcagtga
     7681 atttgaaatc aacggtggta cggtaaacaa cacctgcgcc ccttacgaat tggttcggac
     7741 gatgcgggcc tcgattttgg tgctggggcc gacgctggcg cgtttcggcg aggcgcaagt
     7801 cagcctgccg ggcggctgcg ccatcggttc gcgccccgtc gatcagcatt tgaaaggctt
     7861 ggaagcgatg ggtgctgaga ttgttatcga acacggttac gtcaaagcca aaggcaaact
     7921 caaaggtacg cgcgtggcga tggatgtcgt taccgtcggc ggcacggaaa acctgctgat
     7981 ggcggcgacg ctggcggaag gtacgacggt tttggaaaac tgcgccattg agcctgaagt
     8041 ggtcgatttg gcggaatgcc tggtcaaaat gggcgcgaaa atcagcggca tcggtacgtc
     8101 cacaatgatt gtggaaggtg cggacgagct gcacggctgc gaacacagcg tcgtccccga
     8161 ccggattgag gcggggacgt tcctgtgcgc ggtggcgata accggcggca gggtggtttt
     8221 gcggaatgcc gcgccgaaaa cgatggaagt ggtgttggac aaactggttg aggcaggtgc
     8281 ggtgattgaa gcgggcgacg attggatcgc catcgatatg cggcagcgtc cgaaggcggt
     8341 ggacatccgc acggtcgtcc accccggctt ccccacggat atgcaggcgc agtttatggc
     8401 attgaatgcc gtggcggagg gaagctgccg cgtggtggaa acgatttttg aaaaccgctt
     8461 tatgcacgtc cccgagttga atcgaatggg ggcgaacatt acaaccgagg gcaatacggc
     8521 atttgtgcag ggtgtggaac agctttccgg cgcggtcgtt aaggcgacgg atttgcgtgc
     8581 gtccgccagc ctcgttatcg ccggtttggc ggcgcgaggc gaaaccgtgg tcgaacagat
     8641 ttaccacttg gatcgcggtt atgaaaatat tgaaaaaaaa ctcggcagcg tcggcgcgaa
     8701 aatcgagcgc gtatccggct gatcgggttg cgatgccgtc tgaagccttc agacggcatc
     8761 aattgtttct gtatcgcaaa cactgattga aaaggactga ttatggcatt tttaaaactg
     8821 accgaacaaa acgtgcaggg caaaaccgtc ctcatccgcg ccgatatgaa cgtgccgttc
     8881 aaagacggca aaatcagcga cgacacccgt atccgcgcct cgctcgcgtc catccaatac
     8941 tgcatggaca acggcgcatc cgttatcgtg atgatccact tgggtcgccc gactgaaggc
     9001 gagttccacc ccgaagacga cgttgcgccc gttgccgcgc atttgggcgg tctgttgggc
     9061 aaagacgtga aagtattgaa cgactggcgc gaaaacaaac cggctttgaa cgcgggcgat
     9121 gtcgtgatgc tgcaaaacgt gcgtatcaac aaaggcgaga agaaaaacga tttggaactg
     9181 ggtaaagcct acgcttcttt gtgcgacgtg ttcgtcaatg acgcgttcgg caccgcgcac
     9241 cgcgcccaag cttcgaccga agccgtcgct caagccgcgc ccgttgcctg cgccggcgta
     9301 ttgatggcgg gcgaactcga cgctttgggc aaagccctga aacagcccgc gcgcccgatg
     9361 gttgccatcg ttgccggcag caaagtgtcc accaagctga ccatcctcga atcgctggcg
     9421 gataaagttg accaactcat cgtcggcggc ggtatcgcca acaccttcct gttggcggaa
     9481 ggtaaagcca tcggcaaatc tttggcggaa cacgatttgg tggaagaatc caaaaaaatt
     9541 atggcgaaaa tggcggccaa aggcggttct gtgccgctgc cgaccgatgt cgtcgttgcc
     9601 aaagcctttg ccgccgatgc cgaagcggtc gtgaaagaca ttgccgacgt tgccgaagac
     9661 gatatgattt tggacatcgg tccgaaatcc gccgccgagc ttgccgaact gctcaaagcc
     9721 gcaggcacgg tcgtttggaa cggccctgtc ggcgtgtttg agtttgacca attcgcaggc
     9781 ggcacgaaag ctcttgccga agccattgcc caaagcaaag cattctcgat tgcaggcggt
     9841 ggcgacacgc tggcggcgat tgctaaattc ggcgttaccg accaaatcgg c
//
LOCUS       DAWHXZ010000058         9798 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000058 DAWHXZ010000000
VERSION     DAWHXZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.058"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(12..1307)
                     /locus_tag="WIE35_000911"
     CDS             complement(12..1307)
                     /locus_tag="WIE35_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ4153843.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVAGAAAEAGAQIFEQSPMTRIEP
                     HQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFQPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     DDVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1433..2320)
                     /locus_tag="WIE35_000912"
     CDS             complement(1433..2320)
                     /locus_tag="WIE35_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ4153844.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2320..3285)
                     /locus_tag="WIE35_000913"
     CDS             complement(2320..3285)
                     /locus_tag="WIE35_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ4153845.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWSTLNPFGDGENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3301..4425)
                     /gene="potA"
                     /locus_tag="WIE35_000914"
     CDS             complement(3301..4425)
                     /gene="potA"
                     /locus_tag="WIE35_000914"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ4153846.1"
                     /translation="MTATTASSTKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLSNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4621..4728)
                     /locus_tag="WIE35_000915"
                     /pseudo
     CDS             complement(4621..4728)
                     /locus_tag="WIE35_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4807..5076)
                     /gene="rpsO"
                     /locus_tag="WIE35_000916"
     CDS             complement(4807..5076)
                     /gene="rpsO"
                     /locus_tag="WIE35_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ4153847.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5299..6234)
                     /gene="secF"
                     /locus_tag="WIE35_000917"
     CDS             complement(5299..6234)
                     /gene="secF"
                     /locus_tag="WIE35_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ4153848.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGTALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6238..8091)
                     /gene="secD"
                     /locus_tag="WIE35_000918"
     CDS             complement(6238..8091)
                     /gene="secD"
                     /locus_tag="WIE35_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ4153849.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8303..8569)
                     /gene="yajC"
                     /locus_tag="WIE35_000919"
     CDS             complement(8303..8569)
                     /gene="yajC"
                     /locus_tag="WIE35_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ4153850.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8697..>9798
                     /locus_tag="WIE35_000920"
     CDS             8697..>9798
                     /locus_tag="WIE35_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ4153851.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAVAEYRLNRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRAFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2495 a         2609 c         2439 g         2255 t
ORIGIN      
        1 gcctgcgacg cttaacggct gtctttccac tgataatatt tcgagccgag gaaagaaccg
       61 agtttgcgca aaaacggttg caggataata ttcggctggc gcaaccgctc aaacggcttc
      121 agacggcatt cgtcccctaa aattgcttcg gcaaccgcca gacctgcaat gcctgttatc
      181 gccatcccgt gtccggaata accttgtgca taaaaaacat ttgcggccaa acgtccgaaa
      241 tgcgggacaa ggttggcggt aatgtcgcac tccccgcccc acgaatattc gattttgaca
      301 tcgtcaagct gcggaaaaac tttaagcata tcttggcgga caagctcggt catacgcgca
      361 ggattgtcga taaactcgtt atccttaccg ccgaaaagca gtctgccgtc cgcgctgagg
      421 cggtaataat ccaaaatatg gcggttgtcg catactgcca tattgttacg gataagccct
      481 tttgcgcgcg cccccaaggg ttcggtcgca ataataaagg tgctaacggc aatcgccttg
      541 cgttccaaag gctggaatat cgggttcaaa cctgcatagg tattgacggc atacaccaga
      601 tttttgcatt cgacgctgcc ttcgggcgtg taaaccagcc aaccgttttg atgcggttcg
      661 atgcgcgtca tcggggactg ctcgaaaatc tgcgcgccgg cttcggcagc ggcaccggca
      721 acacccaacg tgtaattgag cggatgcagg tgtccggata aggggtcgaa ttgtgcccct
      781 tggtacatat cgctgtcaag ctgctgtttc aactcggctt tatcccaaag ttgataatga
      841 ctcgcaccgt aatgccgttg ggcgtgttca tgccactgct gcaactcttc ccaatgctgc
      901 ggacggacgg caaccgtggc ataaccgcgc tgccaatcgc aatcaatggc atgtttgcgg
      961 acgcgttcgt ccaccagttc gaccgcctgc aaagactgtt gccaaaacca ttgcgcctgc
     1021 tccaagccga cctgtttttc aatttccccc ataccgcagg cgtaatcgct gataacctgc
     1081 ccgccactcc gtcccgacgc gccgaaaccg atacgcgcgg cttccaacac aaccgtttca
     1141 tgtccctgct ccgccaaggg caatgcagtg cacaaaccac tcaatccgcc gccgatgata
     1201 caggtatcgg ttttcagacg gcattgaagt ttcggataaa cagtatgagg attaaccgaa
     1261 ctgaaataat aagaaggcag atattcttga aaatcagggc gaatcattgt gtttgcttta
     1321 tcaggtgtat tttcggacgg aatgatacag gctgtcgggc catatcgtcc aaacagaaaa
     1381 tcggttgaag aaaacaggct gacccagtca tgcggtcagc ctgccttatt aattaatttg
     1441 ctttctcggc agccaatttt tcttggcggt aggcttctgc cgcttctcgg tcacgcttgg
     1501 ttgcctgcct catcatccaa taattgacga tgatgaccaa tgttccgatg atgccgatta
     1561 ggatggtcgc caagacattc atctgcggat cgagacccaa cttgattttg gagaaaatca
     1621 cctgcggcaa tgtggatgaa ccggggccgg agaggaatga ggtaatcacc aaatcatcca
     1681 aagacagggt aatgccgagc agaaagcctg aagcgatggc aggggcaatc aaaggcaaag
     1741 tgatgacaaa aaagattttc agcggacgcg cgcccaaatc cattgcggct tcttcgagcg
     1801 actggtcaag ctcaaccaga cgcgaacgga taacaacggt aatgtacgcc atacacagcg
     1861 tcgtatgtcc gaggaagatg gtgaaaaagc cacgatcgaa gtagagatgt tgtaaccatt
     1921 cgctgccctg caaaaatatc tgtacctgaa taatcagcag cagcatagac agaccggtaa
     1981 tcacgtcggg catcaccata ggtgcggaaa tcataccggc gaacaaggta ctgccgcgaa
     2041 aacgtttaat ccgcgccatc gcatagcctg ccagcgtgcc caaaacgacg gcagcaagcg
     2101 aagacacaac ggcaatccgc agcgacagcc aagcggcttc caagatggtg tcgttttcca
     2161 gcaatgcgcc gtaccacttg gtcgaaaagc cgccccaaac ggttaccagc ttggattcgt
     2221 taaacgaata gatgaccaaa acaaccagcg ggatatacag aaacgccagc gacagtgcca
     2281 acatcagttt caagaaccaa gataatttgg atttctgcat tatttggctc cttcttccaa
     2341 ttcgcggttt tcataatgct gaaacagggc aatcggcacg accagcagcg cgaccatcac
     2401 gacggcgacg gcggaagcca gcggccagtt gttttgatcg aagaacgcct gccacaagac
     2461 tttaccaatc atcaggtttt ccgaaccgcc gaccagctcg ggaatgacga actcgccgac
     2521 agcagggacg aaaaccagca tggagcctgc aataatgccg gttttcgaca aaggcagggt
     2581 aatcgtcaag aacgatttga ccggccccgc gcccaaatcg gaagccgctt caagcaggcg
     2641 gttgtcgagt ttcaccagtt gcgtgtatag cggcagaatc ataaacggca gataggcgta
     2701 aaccatcacc aaattgagcg aaaaggcatt gtagaacaaa tccaaaggct cgctgataat
     2761 acccatttta atcaacaggt tgtttacaat gccgttatgc ccgagcagac ccatccacgc
     2821 atagacgcgc aacaggaacg atgtccaaaa gggcagcata atggcaagca gcaaaccatt
     2881 gcggacagaa ggattggcac gagaaatcgc ataggcggtc ggataaccga ccaacagaca
     2941 aattaccgtc gtagtcagcg cagtcttaat tgaagaccaa taagtcatca gatagatatt
     3001 gctgttttca ccgtcgccga acggattgag cgtactccaa aaattttgga agatgtctgc
     3061 ataattttgg tagctgacag caatattcag acgacccaaa tcctcatcta tcgtcgttaa
     3121 aggagtaaac ggcgggatgg cgatttcttg ttcggcaaag ctgattttca gcacgatggc
     3181 gaacggaatc agaaacagca ccaaaagcca aatatacggt acggcaatca ccgcacgctg
     3241 ccccggacgg cggaacagtt tgtttttcag tttattaagg ttcattgcat tccccttaaa
     3301 tcaacggaac agtggcgtag gctggttttc cggccagctg atatagacgg tttcgtccca
     3361 agttggcggt gtaatgttgc gcacatacca gtaaggggcg gggacttggc tcttgacgac
     3421 gcgcccgttg gagagtttga tatggtatat ggcgaagctg cccaaatagg cgatttcttt
     3481 taccgtgcct ttcgcccagt tgtagtcgcc caaatattcg ggtttttctt tatataaatc
     3541 aatatcctct ggtcgaatac taacccaaag gtcctgctcg ctcggaccac ccaaaccgtg
     3601 gtcgatgcgg acgtggtttt ccaaaccttc gcattcgata acggcatagt cggcatgatc
     3661 ttcaatcacc acaccgtcaa agatgttggt ttcgccgata aactcggcag tgaagcggct
     3721 gttgggatag tcgtacacgt cgctgggtgt gccgacttgc tgcaactgac cgtcggacat
     3781 aatggcgatg cgggtcgcca tcgtcatcgc ctcttcttgg tcgtgtgtaa ccataataca
     3841 ggttacgccg acttgttcca gcgtattgac caactcaagc tgggtttgct ggcgcagttt
     3901 tttgtccaac gcgccgaggg gctcatccag cagtagaatt ttcggacgtt ttgccagact
     3961 gcgtgccaaa gcaatgcgct gctgctgacc gccggacaat tggtgcggtt tgcgtttagc
     4021 aaatttggtc atctgaacca ggcggagcat ttcttcgacg cgcgcggcga tttcgccttt
     4081 aggcattttg tcctgtttca gaccgaaggc aatgttttgt tctacggtca tatgcggaaa
     4141 aagcgcgtaa ctttggaaca tcatattgat ggggcgatca tagggtgcaa gtttggtaat
     4201 atcctgacca tcaaggataa tttttccctg attgggactt tccatacccg ccagcatacg
     4261 cagcagcgtg gattttccgc tgccggaact gcccaaaaga gcgaaaattt cgtgttgata
     4321 aatgtccaag tcgatgttat cgacagcgta attgtcacca aactttttca ccaaaccttg
     4381 gatttgcaga taaggcttgg ttgaagacgc agtggttgcg gtcataatgg caatactcca
     4441 ataaaaagac gagtaccggc aaaacggatt ttcgaatggg tgataaaaag ctgtttgatt
     4501 gctggcggga gtcgcaacgt ctgatgccgt ctgaaactct tgtgaagcgc acgggcagca
     4561 tgaaatggaa caagattcca aagaacttta tattatatta gtttatgcgg ttttcgggca
     4621 atatagtgga ttaaatttaa accagtacag cgttgccttg ccttgccgta ctatttgtac
     4681 tgtctgcggc ttcgtcgcct tgtcctgatt taaatttaat ccactataaa tatggatttg
     4741 agcttgtcgg aaagcaacag aaaagaaaac accgcccatt tttctgggcg gtgtcggaaa
     4801 gcgtaattat ttacgcagac ccaagcgggt aatcaacgcg cgatacgtat cgggctgggt
     4861 acggcgcaag taggccagca ggcggcggcg ttggctgacc attttcaaca ggccgcgacg
     4921 gctgtggtgg tcttttgggt tggctttgaa gtggggggtc aggtcgttga tgcggaaagt
     4981 caacagagcg acttgtactt cggaagagcc ggtgtcgcct tctttgcgtt ggaaatcttt
     5041 aacgatttgt gctttttgtt ctacggtcag tgccataatg aaaactccaa aaatataaga
     5101 atccccatag ggattcagac aagtttgcca agcctgaaga caacggcaaa ctccctatgc
     5161 ccaagatagg acaacgtggc attatgacac aattcccgca tttctgcaca atattttaag
     5221 accgtggaag taaccgttaa aaatgccgtc tgaagcattg tctgcttgag acggcaatgt
     5281 tcagacggca tatgcgcttc aaaccacgat ttcttctttc ttcttcggtt ctttaccgat
     5341 attgtcgcgg ctcaaaccga acattagcag aagcgggctg gcaaccaata cggaagaata
     5401 aatgccgaac acgatgccga tggtcaacgc catagaaaag ccgtgcaagg ccgtaccgcc
     5461 gaacaccagc atggatacga ccatcgcctc ggtcgaaccg tgggtaatga tggtgcggct
     5521 catcgttgcg gtaatcgcgt tgtcgatgac ttccggcacg gcatgtccgc gcatcgccgg
     5581 cttgcggaag ttttcacgga tacggtcgaa gacgacgacg gattcgttca cagaatagcc
     5641 caatacggca aggatacccg ccaagacggt cagcgaaaat tcccattgga agaaggcaaa
     5701 gcagccgaga ataatcacga tgtcgtgcat attggcgata atggcagata cggcaaaacg
     5761 ccattcgaaa cgcatcgaca ggtaaataat gatgccgata acgacaaaac ctaaagccat
     5821 caatccatta cttaccaatt cctcaccaac ttgcgggccg ataaattcga cttggcgcaa
     5881 agtaacgtcg ggactgtctt ttttcagcaa atccataacc tgattggaca actgtgcgga
     5941 agtaacacct tctttgttcg gcaggcggat catgatgtgt ttgttcgtac ccaatgcctg
     6001 aacctgtaca tcacctattt tcagcgtatc gaggcgttcg cgcatcttat tgacatccgc
     6061 accctgctga tattggactt ccattaccgt gccgccggta aattcgacag agaaattcag
     6121 acctctggta accaaaaaga atacggcggc aataaaggta accaacgaaa taaaggtcgt
     6181 cagtttgccg tagctcataa acggaatatc gcgtttgatt ttaaagagtt ccatagctta
     6241 ctccttgcct cctgccattt cggctttcgg cttccacacc gaaccgatgg aaatattctg
     6301 caatttgcgt ctgcgtccat accacagatt gaccaacgca cggaatacga cgacggacga
     6361 atacatcgaa gtcagaatac ccaaacagtg tacgaccgca aaaccgcgta ccgggccgga
     6421 accgaatacc aaaagcgcga taccggcaat cagcgaagtc aggttggaat cgacaatggt
     6481 cgcccatgcg tgttggaaac cgagattgat tgcctgctgc ggcggcacgc cggcacgcaa
     6541 ttcttcgcgg atacgttcgt taatcaagac gttggagtcg attgccatac ccaaagtcaa
     6601 cgccagcgcg gccatacccg gtaacgtcaa cgttgcctgc atggcagaca aaataccgat
     6661 taggaacagt atgttggcac tcaatgcaat ggtagaaaag aaacccatca gacgatagta
     6721 aaccaccatg aatgcagcaa cgatggcaaa accccataaa gtcgaatgga agcctttttc
     6781 gatgttctcc ttacccaaag acggaccgat ggtacgttct tcgacaatct gcatcggcgc
     6841 ggcaagagaa ccggcacgca acagcaaaga cgtatcattg gcttcggctg tcgtcatgct
     6901 tccggaaatt tccacgcgtc cgccggtaat ggcagtacgg ataaccggcg cggttacaac
     6961 ctcggatttt ccttggtcga tcaaaaccat cgccatgcgt ttgccgacat ttgcggcagt
     7021 cagttcgcca aaaatgctgc cacccgcgct gtccaagctc agactgacag caggtgcgcc
     7081 catttggtcg aaactcggtt gcgcatcgtt gatgttgtcg cccgtcagct cgacctgttt
     7141 gctgattagc agagtttcgg gacgatctcc gccgcttgaa agcagctcat aaccgctcgg
     7201 cacgttgcct tccaatgcct cgcgcaactt ggcaggatcg tcctccacca tacgcaattc
     7261 caaagtcgcg gtacggccga tgatgtcttt tgccttggca gtatcctgaa cgcccggaag
     7321 ctgcacgacg atacggtcgg caccggactg ctggatgacg ggttcggcca cgcccaactc
     7381 gttcacacgg ttgtgcaggg tagtgatgtt ctgtttgacc gcatcggaac gcactttatt
     7441 gaccgcctct tccgaaagcg tcaagacgat attgctgccg tctgaattca gcgttgcttc
     7501 aggaaacagc ttgcgcaact gcggcagtgc cttttgcaca tcacctgcat cctgcaaagg
     7561 gacggtcagg ctgtttccag cctgacgcac cgtgccgctg cggatttttt cgcggcgcag
     7621 ttcgcggcgg atgtcgcccg aataacgttc aaacgttttc tgcatcgccg ctttcatatc
     7681 gacctgcatg gtgaaatgca cgccgccgcg caggtccaaa cccaaaaaca tcggattggc
     7741 tttgattttc gccatccatt cggggctgtc cgccaacagg ttgagcgcgg taatataccc
     7801 ttcgcccaaa gtgttttcga tgacgtcgcg cgctttaagc tgcgtttctg tgtctttgaa
     7861 acgcactttc agtgaattgt ccacaacaaa catcccgtcg gtctgaatac ctgcgttttt
     7921 cagcgcggca tccactttgg attgagtctg ttcgttgatg atgatggctt gtcggttggt
     7981 cgatacctgc acggcgggtg tttcgccgaa taggttgggc agcgaataca ctgcggcaac
     8041 cgcaatcgtg aacacaatca gcagatattt ccataaagga taacggttca tcattgttcc
     8101 ttaatggttg gaaccccacc ctttcggtgg tgtcggaatc gggctatttc agaagaggca
     8161 aaaacccttc ccagccaggc aagaccggaa ggcggcatct tgaatatgcc gccctgcgtg
     8221 tcggaacatg gtcaagcctt cggttggaat tcaaaacaaa gtgccgcatt cgggctttcc
     8281 agatgcggct tgtcggcaca aatcaatcga cttttgcggc aatcgcattg cgttctactt
     8341 cgacctcgat ttttgtaccc tgtccgatat ccacggtaaa aaactgttcg ccgactctgg
     8401 ttaccttacc cttgaaacct gccgccaaga ccactttgtc gccgactttc aaggcggcaa
     8461 gcattgcctg atgcgctttg aatttctttt gctgcggacg catgatcagg aagtagaaca
     8521 ccaccataat caacactaaa ggagcaaatt gtgcaacagc ttgattcata atttatccgt
     8581 tctttctaat atggttgaaa atcgagaggg gtatataata acataagacc gtaaacaata
     8641 tatcgggttt gccgtccgta ccgaccgtat accgcagcct gcccgtccac aaacccatgt
     8701 ccttgacccg cctaatcttg aaattctatg cactgttgcg cctttttttg ggcaaaaacg
     8761 cccgcaccgc atggatttcg catcccgcct gtgccgggca cgaacccggc gcaaaccatc
     8821 ccgattcgcc cgaccgcatc ctctgcatcg agcaggcatt gcgccgcgcc ggtatttggc
     8881 agcacctcca aaccatagag gcggaagaaa tcagcgatac gcgcctcgca cttgtccact
     8941 cgagcaaata tctgaaccgt ttggaatctt gcctgcccca aaaaggcaag atttcccgcc
     9001 tggataacga cactgcaatc agcacaggct cgctgtctgc cgcacgcttt gccgccggtt
     9061 cggcagttca ggcagtcgac atggtcatga accgtaaagc atggcatgcc ttttgcgccg
     9121 cccgcccgcc cggacaccat gcgggcagcg gcaaagccgg cggattctgc ctgctgaaca
     9181 acgttgccgc cggcgtcatg catgccgttg ccgaataccg gctgaaccgc atcgccgtca
     9241 tcgatttcga tgtacactac ggcgacggta cggcagaaat attcaaagac gatccgcgca
     9301 tcctgttttt caacctgttt gaaaccgacc ttttcccctt ccccgaaaac aacgatatgc
     9361 ccgacggcgg caatatggtg cacctgccct tgccgccggg aacgggcagc cgcgcattcc
     9421 gcgaagccgt ccgcagacag tggctgcccc ggcttgccgc attcaaaccc gaactggtgc
     9481 tgctgtcggc aggattcgac gcacaccgtc tggacgaatc gggcaggctt aacctgcacg
     9541 aggcggattt tgcctggctg acacacaaaa tcattcagac ggcatcgggc tgccctggca
     9601 aaatcgtatc cgtactcgaa ggcggctaca cccttgaacc tctggcacaa tctgctgccg
     9661 agcatatccg cgtgctggca gggctgggca aatccgatgc cgcaaccgcc tatcagaaaa
     9721 cactggaccc aacaaaaaaa cggtttgcca aaccgaaaac cggacaggtg cgccaaccta
     9781 cccaatccga ccgttacg
//
LOCUS       DAWHXZ010000059         9715 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000059 DAWHXZ010000000
VERSION     DAWHXZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.059"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..164
                     /locus_tag="WIE35_000921"
     CDS             <1..164
                     /locus_tag="WIE35_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153852.1"
                     /translation="PVLKDCVYFHRRFKGRRYSADGAQTAFRRHFHSFGVSVRIALPV
                     RAISPCGLP"
     gene            complement(239..1702)
                     /gene="guaB"
                     /locus_tag="WIE35_000922"
     CDS             complement(239..1702)
                     /gene="guaB"
                     /locus_tag="WIE35_000922"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ4153853.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(1843..4401)
                     /gene="glnD"
                     /locus_tag="WIE35_000923"
     CDS             complement(1843..4401)
                     /gene="glnD"
                     /locus_tag="WIE35_000923"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ4153854.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAALETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGISDARQFAADHF
                     LTEEESDLLAWLVENHLLMSIVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLAGNGGNPHALFGRRQQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETSNHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEIFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQTLLEQLS"
     gene            complement(4450..4770)
                     /locus_tag="WIE35_000924"
     CDS             complement(4450..4770)
                     /locus_tag="WIE35_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4153855.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(5094..5567)
                     /gene="bfr"
                     /locus_tag="WIE35_000925"
     CDS             complement(5094..5567)
                     /gene="bfr"
                     /locus_tag="WIE35_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ4153856.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(5595..6059)
                     /gene="bfr"
                     /locus_tag="WIE35_000926"
     CDS             complement(5595..6059)
                     /gene="bfr"
                     /locus_tag="WIE35_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ4153857.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVSRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(6523..7506)
                     /gene="lipA"
                     /locus_tag="WIE35_000927"
     CDS             complement(6523..7506)
                     /gene="lipA"
                     /locus_tag="WIE35_000927"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ4153858.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(7499..8122)
                     /gene="lipB"
                     /locus_tag="WIE35_000928"
     CDS             complement(7499..8122)
                     /gene="lipB"
                     /locus_tag="WIE35_000928"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ4153859.1"
                     /translation="MKVIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(8124..8399)
                     /locus_tag="WIE35_000929"
     CDS             complement(8124..8399)
                     /locus_tag="WIE35_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ4153860.1"
                     /translation="MTEQKNKTSLIEFPCTFSLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQIKVENQEQLDNIYRALTSHELVKVVL"
     gene            8584..9654
                     /locus_tag="WIE35_000930"
     CDS             8584..9654
                     /locus_tag="WIE35_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ4153861.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLAFALGDTLAPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNTLKAWFPVLMRQGSNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
BASE COUNT         1922 a         2281 c         2915 g         2597 t
ORIGIN      
        1 cgcccgtatt gaaagattgc gtttatttcc accgccgttt taaaggccgg cggtattcgg
       61 cagacggggc gcaaacggcg ttcagacggc attttcattc tttcggcgtg tccgtccgaa
      121 ttgctttgcc cgtccgcgca atcagcccgt gcggcttgcc ttgaacggac aaaaaatgcc
      181 gtctgaaacc cgaaaatcag gtttcagacg gcattttgcc ttgaaaaggc tgttcaaatc
      241 agcggtggta gttcggtgct tctttagtga tttgaacgtc gtgaacgtgc gattcgctca
      301 tacctgcgga agtgatttcc acaaattcgg ctttttcgtg catttcggca atattggcgc
      361 aacccaagta acccatactg gaacgcagac cgccggtcag ctggtggatg atgttcacaa
      421 tcgggccttt gtaaggaacg cggccttcga tgccttcggg gacgtatttg tcggtgctgt
      481 cggttttgtc ttggaagtag cggtcggcag aaccttggct catcgcgccc aatgaaccca
      541 taccgcgata ggatttgtac gagcggcctt gatagagttc gatttcgccc ggcgcttctt
      601 ccgtgccggc aaacataccg ccgagcatga cgctgtacgc gcctgcggcg agggctttgg
      661 cgatgtcgcc ggagaagcgg atgccgccgt cggcaatcag gggaacgcct gtgcctttga
      721 gggcttcggc aacgttgtgg atggcggtca gttgcggcac gcccacgcct gccacgatac
      781 gggtggtgca aatcgatccc gggccgatac cgactttgac ggcatccgca ccggcggcga
      841 ccaaatccaa agcggctttg gcagtggcga tattgccgcc gatgacttgg atgtgcggat
      901 aggtttcttt cacccatttc acgcggtcaa tcacgccttg gctgtgcccg tgggcagtat
      961 cgacgacaat cacgtccacg ccggcttcga ccaaggcttt gacgcgctct tcggtatcgc
     1021 cgccggtgcc gactgccgca ccgacgcgca gacggccttc ggagtctttg ttggcgttgg
     1081 gaaactcggt ggtttttaaa atatctttaa cggtaatcag acctttgagt tcgtcttttt
     1141 cgttcagaac caaaacgcgc tcgactttgt gcgtgtgcat cagttcgcgc gcttcgtcta
     1201 tgcttgtgcc ttcggggacg gtaaccagac gttcgcgcgg ggtcataatg gcggaaacgg
     1261 gcaaatcgac gcggttttca aaacgcaggt cgcggttggt tacgatgccg acgactttgc
     1321 cgttttcaac gacgggcagg ccggacattt tgcgtttgcg ctgcgcacgc atttccaaga
     1381 cttcgcggat gagcgtcgtc ggtgcaacgg ttacggggtc tttgaccacg ccgctttcgt
     1441 ggcgtttcac tttggaaacg gcgcgcgcct gcatttcggg cggcatgttt ttatggatga
     1501 tgccgatgcc gccttcctgt gccatcgaaa tggcgaggcg cgcctcggta acagtgtcca
     1561 tcgcagcgga aagcaggggg aggttgagtg tgatttcgcg ggtgagcttg gtttgaagtt
     1621 taacgtctcg cggcagcacg gtcgaatgtg cgggaaccaa caaaacatcg tcgaaagtat
     1681 aggctttttc tacgatacgc ataatgctcg gtctttcagt ttgtgcaaga tgcacggcat
     1741 tatagcacgt taccggcggc ttgacagttt atattgtttg caatatcccc cgccttcagg
     1801 cggcatacgg cgtggcggcg gaatgccgtc tgaaggcggg cgttatgata attgttccag
     1861 cagcgtctgc ttcaatgagg actggatttt tgggtttttc aggtcggggc taaaaacggt
     1921 aaaactgtct tcggcgcgtt catccagtgt ggagattttg gcatagcgca ggctgacgtt
     1981 gtgggcgaaa aagatttccg ccatatcggc gagcaggaag gggcggttga cggcggtaat
     2041 ttcgacggag taccagtcgg ggtagtcttc ttcgggggta atggtgatgc ttggcgcgat
     2101 cggcatgtag cggctgcggc ggctgatgcg gcggctgtgg tttgaggttt cggcaacggt
     2161 gtgtccgtgg ataaagctgt tgagttcggc ttcaagcgcg ctttgaatgt cggggtagtc
     2221 ttcgggggcg tgttgtgagg gaatttgcac gatgaaggtg tcgaggatgt agtcgtgttc
     2281 ggtgataaag gcgcgggcgg cgaggatatc gaagccgtgg cggctgaaaa tgcggcagag
     2341 acgggcaaac aggcgcgggc cgttgggcat gaaaaccatg acttgaaagc tgtcgctttt
     2401 gggcaggatg cggctgcgga cgatgggggt ttcaaagtcg tggactaggt tggcggcgtg
     2461 ccacaggatt tcgcgggact ggtggcgggc gaagtaggcg gaaccgagcg cgttccaaag
     2521 ctttttctgc tgtttttcgg ggacggcggc gcgggtgagc aagtcggcgg cttcttgttg
     2581 gcggcggccg aagagggcgt gcgggttgcc accgttgcct gcgaggtagc gtccggcggc
     2641 atggaagagg ctttccagca ggctggcgcg ccatgcgttc cacagcttgg gattggtacc
     2701 gcgtatgtcg gaaatggtca gaaggtagag cgcgctgagg cgttcgtggg tttggacgcg
     2761 tttgcagaag gcatcgagta cgtcggggtc ttggatgtct tctttttggg cgacgatgga
     2821 cataagaaga tggttttcga ccagccaggc aagcaggtcg ctttcttctt cggtcaggaa
     2881 gtggtcggcg gcaaattggc gcgcgtctga gatgccttgt atggcatggt cgccgccgcg
     2941 tcctttggcg atgtcatgga aaaaggcggc aaggtagagg atgtcttgtt tttcaaagga
     3001 ctgcatcagt gcagaggcgt agggcagctc gtggctgtgc atatccaagg caaggcggcg
     3061 gacgttgcgg acgacggcga ggatgtggtc gtccacggga tagatgtgga acaggtcgtg
     3121 ctggagcagg ccgacgattt tttcccacgc gggcaggtag cgtcccaata cgccgtagag
     3181 gttgaggaag cgcagggttt gggtcagtcc gttgccgttg cggaaaaagc cggcgaagcg
     3241 gtggcggttt tcagaatttt ggtagaagct gcggttgatt ttgcgcgtcg cgccccacca
     3301 tgcgcgcagg gtttgcggtt cgagcgcggt aatgtcgttg cgctgctgca tgatttcgac
     3361 gattttgaaa atgtgttcgg gccgtctgaa aaaaatatcg gtgtgccgcg cggcgatttg
     3421 gttgttgact tggatgtagt cgtcgtcaat ccgcagggta acgcgcaacg gggtggagga
     3481 aacgcggctt tgcagcatag gcgtgaggat gccgcccagt tgtttgacgg ttttaatcgc
     3541 gcggtaaaac acgcgcatca gttcttcgct ttggcggcgg aggttcaagc cttcataacc
     3601 catgctttcg gcgacttgcg gctgcaaatc gaacagcagg cggtcttcgg cgcgcttggc
     3661 gtttaaatgc agatggatgc ggatgtgggc gaggcggcgg tagccgtgcg aaagcatacc
     3721 ggcttcggca cgcgtcaaaa tccgctgttt gagcaggtcg ggcaggtcgg tcgccaagcc
     3781 ttgcgccttc gctatccaaa gcagggtgtg gatatcgcgc agaccgcccg gacagctttt
     3841 gatattcggc tccaatactg cccccgaacc ttgcgatttg gcgtggcggt gttccatctc
     3901 caccagtttt gcctcgacaa acgccgccac attgcgctgc gcgttcattt tttccgctag
     3961 tttatccgcc gtttggcggt tgccaaacaa aaacctagcc tctaaaaacg ctgtgtcccc
     4021 cgtaatatca ttgcgcacgc tttcacatag ttcatcaacg ctgccgcttt ttacagacgg
     4081 catcagtttg cagtcccaca gggtttggac aaaccgggca atctgttcct gaatgccgtc
     4141 tgaaagcggg gcaggggaga caaccgccaa atccacatcc gaacagggat acagttcgcc
     4201 gcgtccgaag ccgcctaccg ccatcaggca taacgcgctg ttttgaaaat actctgccca
     4261 caatgccgcc agtaaggttt cgagtgccgc cgtatattcc ctgaaaaata ccgacacgcg
     4321 gttggctttc aaataatgcg cttcggcggc atcgcgctgc tgtttgaagg tttccagtgc
     4381 tgaagacagg tttgcaggca tttttattct ttcgattggc gggaaaaagg gaggcggatg
     4441 gttcggcggt caaataccgc tttcagacgg catttgtcgg gtatcggcgg aattggtcat
     4501 cagcttcaac cgttcctgcg gcggcagcag caactgatac gccgccacgc ccaaagccgc
     4561 cgccattttt tcgatattcg acagggcgat gttccagcgt ttgcgctcga ctgccgacac
     4621 ataagtcctg tccaaaccgc attgccgcgc caattcttct tgcgaccaac ccttgttcac
     4681 gcggaaaagc cgcatattgt atgccaatac cgcccgcaaa tcctgttcgt caggcagttc
     4741 ggcaggcaga gtcaatttgt tgcccatcat ttgcttccag ataaatggtt aaagttaagc
     4801 atttgctgtt acggatttta cttcacataa aagccaaaaa aaaggggtgg cggcagccac
     4861 acccaaacac acacacatca agaggaaaga gataaaatca gacagattgg aaaacagcaa
     4921 acaagcaccc tgaatttcat atcggtatta tcaaacaaaa acccttatca ggtattgatt
     4981 caaatcaatt tagattttat tcgcaaaccg aaaaagaaaa acggcatatc cgtttatacg
     5041 gatatgccgt ctgatggtgc gggggactgc tgtatttggc agtagtttgt gttttagtcc
     5101 tcttgcgccg ctgtttgcag gtagttgggc agaccgattt tgccgataag ctcttgctgg
     5161 gtctccagcc agtctatatg ttcttcgttg gtgtcttttt gtttttccaa caaatcgcgg
     5221 ctgacgtaat cctgttgcgc ttctgctgtg gcgatggcgg caagcagggc ttcgtgtttt
     5281 tcctgttctt tggtcaaatc gcaggcgatg atttcttcgg tggactcgcc aatcagaagc
     5341 ttgcccagtt cttgcaggtt cggcagacct tcgaggaaca gaatgcgctc gatcaaatcg
     5401 tcggcagctt tcatttcaac gatggactgt ttgaagaaat gttcgcccag ttcttcaaag
     5461 ccccagtttt tcaaaatacg ggcgtgaagg aaatattggt tgatggttac cagcagcaag
     5521 cctaagtttt tgttcagctc gcggataacc aaacggtcgc ctttcatacg gactcctttt
     5581 attccgaata cggattacag ttggctttgg tagtagttgc cttcgccaat cagctcgatc
     5641 aggcgcagct gctgttccag ccagtgggcg tggtcttctt cggtatcttt cagttgggcg
     5701 accatcaaat cgcggctgac atagtcttgc gcctcttcgc acagtttgat gccttttttc
     5761 aaagcgtcgc gcacttcgta ttcggtttgc aggtcggctt tgaggcagga aaccacgtcc
     5821 gtgccgatat tcagctcggc gcgcgccatt ttcggcgtac cgcccagcat caggatgcgt
     5881 cggatgaagt cttcggcgtg tgtggtttct tcttccatct cgtgattgag acgttcaaaa
     5941 agtttggtgt agccccattc ggagtagagg cgggagtgga taaagtattg gtcgcgtgcc
     6001 gccagctcgc cagacagcaa ttcgttcata taatcaacaa cagcttgatt gccttgcata
     6061 atatctctct ttccgtaact tggttttcgg tatgtctgaa cagcatctgt gtcgctattc
     6121 gggatgcgtg cattgtatgc aaaagctgtc ggcatgacaa attttctatt taaaatacaa
     6181 acaattatca aaagaaatag ggcgtattcc ccgatgcctt ccaaatcagt atgctgtttc
     6241 ttatcggttt tttgtgctgt taaaactaag aatccatatc atccataaag aacatttaca
     6301 cttgttaacc atagcgaaaa gcaaatactg tacggttttt tatcaaaatt tataatgaat
     6361 cgttctcatt aaccgacaga ttcttttgag attatcggat tttgggaata aattatgccg
     6421 tctgaggggc tttcagacgg catagacaat taaagctgat taaacaacgc ggcagacagc
     6481 agcgtaggaa attatttttt acttcggaac ggtattttgg gtttaatgat gcccgcaacc
     6541 gccgtggctt tcgcgtaagg cttcggcagc ctgttcgtcg gcgtggtagc tggaacgtac
     6601 catcgcgccg atggcggcat tgctgaagcc cagttcgtat gcttcttttt caaagatttt
     6661 gaactgctcg ggcgtaacgt agcgcaggac gggcaggtgt ccgtctgaag gctggaggta
     6721 ctgtccgatg gtaatcattt cgatattgtg cgcccgcata tcgcgcataa tttcacgcac
     6781 gtcttcgtct gtttcgccca agccgaccat gatgccggat ttggtcggga tatgcggcat
     6841 catttcttta taacgtttta ataagtctaa agaatgttga taattagcac cgggacgggc
     6901 ttttctgtac aggctcggat gggtttctag gttgtggttc atcacgtcgg gcggggtttc
     6961 ggcaaggatt ttgagtgcga tgtccaagcg tcctcggaag tcggggacga ggatttcgat
     7021 tttggtgttc gggctggttt cgcggatggc tttgatgcag tcggcgaaat gctgtgcgcc
     7081 gccgtcgcgc aggtcgtcgc ggtcgacgga ggtgatgacg acgtaacgca ggttcatggc
     7141 tttgacggat tcggcgaggt ttctcggttc gtcggggtcg agcatattgg gccgaccgtg
     7201 tcccacgtcg cagaacgggc agcggcgggt gcagatgtcg cccataatca tgaaggtcgc
     7261 cgtgcctttg ctgaagcatt cgccgatgtt ggggcaggag gcttcctcgc aaacggtgtg
     7321 catcttttgt tcgcgcaaaa tgtctttgat ttcaaagaat ttgcgcgatg ggagtttggc
     7381 gcgtatccat tcgggctttt tcagtttttc ctgaaggggg acgactttga tggggatgcg
     7441 cgcggttttg tccgcgcctc tgagtttgat gccgcgtttg gggtcgtcgg ttttgatttc
     7501 actcattgtt gtctgctttc ggtgtgaatt gtgtttcaag gtgtgcggta agtttggcgg
     7561 cgacttcgtc cggcgtgggg cagggttgga caaaatccgc gatttgcgtc atttccatac
     7621 cggcgtagcc gcaggggttg atgtgggtaa acgggcttaa atccatattg acgttgagcg
     7681 caagcccgtg atagacggag ccgtttttga tacgcagccc cagtgaggcg attttgcgtt
     7741 ctccgacata aacgccgggg cgtttggggt ctgccgccgc ttcgatgccg tattctgcca
     7801 atgtggcgat gatgctgttt tcaagcgcgg aaacgatgtt tctaacactg gttttgcgcc
     7861 gtttgaaatc aatcatcgta taaacgacca attgcccggg cccgtgatag gtaatctgcc
     7921 cgccccggtc gatttggacg acgggaatgt cgtcgcgaat cagcaggtgt tcgggttttc
     7981 ccgccagtcc ttgtgtgaac acgggcgggt gttcgacgac ccacagttcg tcttcggtgt
     8041 cggcattccg tccggcatta aaggttttca tcgcttcaaa agtcggcaga tattcgacca
     8101 aacctttgtg tatgactttc atctcaaagt accactttga ccagttcgtg cgaagtcagc
     8161 gcgcggtaga tgttgtccaa ttgttcttgg ttttcaacct ttatctgaac ggtggcgccg
     8221 gtatagttgc ctttgctgct cggacgcgtg gtgatgtggt gcgcctgcgt gtcgggggcg
     8281 tggaggcgga cggtgtctaa aaccgcctgc tcgaactcgg gatgcaccgc gcccattact
     8341 tttaatgaga aggtgcaggg aaattcgatg agggatgttt tgtttttttg ttcggtcatg
     8401 atgtgctgcc ttatcgtgta cggtatgccg tctgaaggcg ggtttgcctt tcagacggca
     8461 tcggatgtgc gttattttag cctaaaccgc gataacaggc tatcgggaag gcggaggctt
     8521 ttttgacggc gcggcggttc tgctatactg gcgcgcaata ttatttttag aagggtggtt
     8581 tttatgtatc ggaggaaagg gcggggcatc aagccgtgga tgggtgccgg tgcggcgttt
     8641 gccgccttgg tttggctggc tttcgcactt ggcgacacac tggctccgtt tgcggttgcg
     8701 gcagtgttgg cgtatgtgtt ggatcctttg gtcgaatggt tgcagaaaaa gggtttgaac
     8761 cgtgcatccg cttcgatgtc tgtgatggtg ttttccttga ttttgttgtt ggcattattg
     8821 ttgattattg tccctatgct ggtcgggcag ttcaacaatt tggcatcgcg cctgccccaa
     8881 ttaatcggtt ttatgcagaa cacgctgctg ccgtggttga aaaatacaat cggcggatat
     8941 gtggaaatcg atcaggcatc tattattgcg tggcttcagg cgcatacggg tgagctgagc
     9001 aacacgctta aggcgtggtt tcccgttttg atgaggcagg gcagcaatat tgtcagcagt
     9061 attggcaacc tgctgctgct tcccttgctg ctttactatt tcctgctgga ttggcagcgg
     9121 tggtcgtgcg gcattgccaa actggttccg aggcgttttg ccggtgctta tacgcgcatt
     9181 acaggcaatt tgaacgaggt attgggcgaa tttttgcgcg ggcagcttct ggtgatgctg
     9241 attatgggtt tggtttacgg cttggggttg gtgctggtcg ggctggattc ggggtttgca
     9301 atcggtatgg ttgccggtat tttggttttt gttccctatt tgggcgcgtt tacaggactg
     9361 ctgctggcaa ccgtcgccgc cttgctccag ttcggttcgt ggaacggcat cctatcggtt
     9421 tgggcggttt ttgccgtagg acagtttctc gaaagttttt tcattacgcc gaaaatcgtg
     9481 ggagaccgta tcgggctgtc gccgttttgg gttatctttt ccctgatggc gttcggacag
     9541 ctgatgggtt tcgtcggaat gttggcagga ttgcctttgg ccgccgtaac cttggtcttg
     9601 cttcgcgagg gcgtgcagaa atattttgcc ggcagttttt accggggcag gtaggcggtt
     9661 ccgaaacata tagtggatta acaaaaatca ggacaaggcg acgaagccgc agaca
//
LOCUS       DAWHXZ010000060         9639 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000060 DAWHXZ010000000
VERSION     DAWHXZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.060"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(129..1175)
                     /gene="adhP"
                     /locus_tag="WIE35_000931"
     CDS             complement(129..1175)
                     /gene="adhP"
                     /locus_tag="WIE35_000931"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ4153862.1"
                     /translation="MKMQAVVVNKNVAGDVEVIEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATYCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1458..1847)
                     /locus_tag="WIE35_000932"
     CDS             complement(1458..1847)
                     /locus_tag="WIE35_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ4153863.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLTEVR
                     TTLLHNAQTMERYYRQKGTFKTYDKNKLKQNEYFKITLSEVSPDHFTLQAAPDPMTNE
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            2073..3251
                     /locus_tag="WIE35_000933"
     CDS             2073..3251
                     /locus_tag="WIE35_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ4153864.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            3317..5251
                     /locus_tag="WIE35_000934"
     CDS             3317..5251
                     /locus_tag="WIE35_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ4153865.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDDIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5664..6446)
                     /locus_tag="WIE35_000935"
     CDS             complement(5664..6446)
                     /locus_tag="WIE35_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ4153866.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAKSAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6585..8774
                     /locus_tag="WIE35_000936"
     CDS             6585..8774
                     /locus_tag="WIE35_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ4153867.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSIQTTFLDEPPLPESWRDLLSFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVGNRFADVPTAVLH
                     SQMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRTFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNKIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            complement(8820..>9639)
                     /locus_tag="WIE35_000937"
     CDS             complement(8820..>9639)
                     /locus_tag="WIE35_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ4153868.1"
                     /translation="SFFVAAYWLVLLLFCARLIWLDRNTDNFALLMLLAAFTVFQTAY
                     AVSGDLNLLRAQVHLNMAAVMFVSVRVSILLGAEALKECRLKDPVFIPNVVYKNIAIT
                     FLLLHAAAELWLPAQTAGFTSLAVGFILLAKLRELHHHELLRKHYVRTYYLLQLFAAA
                     GYLWTGAAKLQNLPASAPLHLITLGGMMGSVMMVWLTAGLWHSGFTKLDYPKLCRIAV
                     PILFAAAVSRTFLMNVNPIFFITIPAILTAVVFTLYLFTFVPIFRANAFTGDPE"
BASE COUNT         2218 a         2492 c         2821 g         2108 t
ORIGIN      
        1 tgacgtgttg cgatacgggt tttcagacgg tatttcaaac agaacagatt aaaatcaaat
       61 ccaaatccat aaaaaatgcc gtctgaacag cgttcagacg gcatttcgat gtgtatcgcc
      121 acgtcaaatc agtggtgatg accgcagccg cattcttttt tcatatcgat caccatacgg
      181 ccggtgattt tgccttcgcg catttcttgg aaaatggcgg gtgcttcatc caaagcacgc
      241 agttggactt tcggcacaac caaaccttcc gcgccgaatt ggaaggcttc ttccaaatct
      301 ttgcgcgtgc cgaccaaaga gccgaccact tcgatgccgt ccaaaaccaa acgcgggatg
      361 gacaaatcca tcgattccgg cggcagtccg atggcaacca cacgtccgcc cgcgcggacg
      421 caattcacgg cagagttgaa tgcggcagca gatacggcgg ttacgaccgc agcgtgtgcg
      481 ccgcctgttt tttcctgaat cactttggca gcgtcctctt tggcggcgtt gacaaccaaa
      541 tccgcgccgg tttctttggc aaacgccagt ttgtcgtcgt tgatgtcgat ggcgacaacg
      601 tgcgcgccga atactttttt cgcgtattgg acccccaagt tgcccaaacc gcccgcgccg
      661 tagatggcaa tccactgtcc cggacgaaca ctggaaactt taatggcttt ataagtggtc
      721 acgccggcac aagtaatgct ggaagcttgc gcaggatcca aaccttctgg gactttgacc
      781 gcgtaatcgg cactcacgat gcagtaggtc gccataccgc cgtcggcggt gtagcccgcg
      841 ttcaatacag agcggcacag ggtttcgcgg ccggtattgc agtattcgca cgagccgcag
      901 ctttggaaca gccaggcgat gctgacgcgg tcgccgactt tcagattttt cacaccgtcg
      961 gcaacttctt taaccaaacc gatgccctcg tgtcccagca cgcggcccgg tttttcgccg
     1021 tagtcgcctg ccgcaacgtg caggtcggtg tggcacacgc cgcaatattc gacttcgacc
     1081 aatgcctcgc cgtattccaa cgggcgaacc tcgcgttcga ttacttctac atcacccgct
     1141 acatttttat tcacaacaac tgcctgcatt ttcatgattg cgctccttag ttacggcaaa
     1201 aaaacctgta aacggaatgt tgtccgatat aagggtaagc atacgcttcc gcatctcaca
     1261 ggtcaagtgg tatgttgttg aaaaatatag attatatgtt atattataac atcttggaaa
     1321 ggcacggcat cggggcggtt gccggatgag gggcggcagg tttcaagttt gaaaaaccgg
     1381 acggcaaacc cgtaaagata ccgtctgaag ctgtgtccgg acggcatctt tacgggtttg
     1441 cgggcttcgg cggaggatta gtcgaagccg gggcaggatt ggtttgtacc ggaagcggca
     1501 atggtaccgc cgtcgttgag cgtaacgacg caggtttcgc cttcgttggt catcggatcg
     1561 ggggctgcct gaagggtgaa gtggtcgggg ctgacttcgc ttaaggtaat cttgaaatat
     1621 tcgttttgtt tcagtttgtt tttgtcgtag gttttaaacg tccctttttg gcggtagtaa
     1681 cgttccatgg tctgcgcgtt gtgcagcagg gtcgtcctga cttccgtcag gcggacgcgc
     1741 cggatgtagg ttttatagga agggtaggtg atgagcgtca ggatgccgag gatggcgacg
     1801 gcaatcatca gctcgagcag cgtaaagcct ttttgaacgt ttttcatagc aatgtgtttc
     1861 catttgtttg tcggtcggcg gcattataac gccgtatcgg aaatggcgga atatgtaaac
     1921 ggattgaaat tttcgggaaa gcagattgta taagccattt aaaacaaatg gttattttta
     1981 ttgtcggcag tttgccgcct tggatggggc agggacttgc ggtagaatcc gcttccgatt
     2041 tatgggattg acgcatacag agaattgaaa acatggcaaa aatgatgaaa tgggcggctg
     2101 ttgcggcggt cgcggcggca gcggtttggg gcggatggtc ttatctgaag cccgagccgc
     2161 aggctgctta tattacggaa acggtcaggc gcggcgacat cagccggacg gtttctgcaa
     2221 caggggagat ttcgccgtcc aacctggtat cggtcggcgc gcaggcatcg gggcagatta
     2281 agaaacttta tgtcaaactc gggcaacagg ttaaaaaggg cgatttgatt gcggaaatca
     2341 attcgacctc gcagaccaat acgctcaata cggaaaaatc caagttggaa acgtatcagg
     2401 cgaagctggt gtcggcacag attgcattgg gcagtgcgga gaagaaatat aagcgtcagg
     2461 cggcgttgtg gaaggatgat gcgacttcca aagaggattt ggaaagcgcg caggatgcgt
     2521 ttgccgccgc caaagccaat gttgccgagc tgaaggctct aatcagacag agcaaaattt
     2581 ccatcaatac cgccgagtcg gaattgggct acacgcgcat taccgcaacg atggacggca
     2641 cggtggtggc gattctcgtg gaagaggggc agactgtgaa cgcggcgcag tctacgccga
     2701 cgattgtcca attggcgaat ctggatatga tgttgaacaa aatgcagatt gccgagggcg
     2761 atattaccaa ggtgaaggcg gggcaggata tttcgtttac gattttgtcc gaaccggata
     2821 cgccgattaa ggcgaagctc gacagcgtcg accccgggct gaccacgatg tcgtcgggcg
     2881 gttacaacag cagtacggat acggcttcca atgcggtcta ctattatgcc cgttcgtttg
     2941 tgccgaatcc ggacggcaaa ctcgccacgg ggatgacgac gcagaatacg gttgaaatcg
     3001 acggcgtgaa aaatgtgctg attattccgt cgctgaccgt gaaaaatcgc ggcggcaagg
     3061 cgtttgtgcg cgtgttgggt gcggacggca aggcggcgga acgcgaaatc cggaccggta
     3121 tgagagacag tatgaatacc gaagtaaaaa gcgggttgaa agagggggac aaagtggtca
     3181 tctccgaaat aaccgccgcc gagcagcagg aaagcggcga acgcgcccta ggcggcccgc
     3241 cgcgccgata aacgaatatg ccgtctgaac acggaaacgg tttcagacgg catttgttat
     3301 tgatttacgg aatattatga gcttgatcga atgtaaaaac atcaaccgct atttcggcag
     3361 cggcgagaac cgcgtccata ttttgaaaga catcagcctg tcgatagaga agggcgattt
     3421 tgtcgccatc atcgggcagt ccggttcggg caagtccacg ctgatgaaca tactcggctg
     3481 tttggatacc gccggttccg gttcgtaccg aatcgacgac atcgaaactg ccaaaatgca
     3541 gcctgacgag ctggcggcat tgcgccgcga acgcttcggt ttcatcttcc aacgctacaa
     3601 cctcttaagc tcgctgaccg caagggacaa cgtcgcgctg ccagccgtct atatgggcgc
     3661 gggcggcaaa gagcgttccg cgcgtgcgga gaagttgttg caggatttgg gtttggcaag
     3721 caaagagggc aacaagcccg gcgaactctc gggcggacag cagcagcgcg tctccatcgc
     3781 ccgcgccctg atgaacggcg gagaaatcat cttcgccgac gagccgaccg gcgcactcga
     3841 taccgccagc ggcaaaaacg tgatggaaat catccacaag ctgcacgaag ccgggcatac
     3901 tgtcattatg gtcacgcacg accccggcat cgccgccaat gccaaccgcg tcatcgaaat
     3961 ccgggacggc gaaatcattt ccgacacctc gaaaaatccc gaaatccccg caagcaatgt
     4021 cgggaggatt cgggaaaaag cctcgtggtc gttttattac gaccagtttg tcgaagcctt
     4081 cagaatgtcg gtgcaagcag tattggcgca caaaatgcgt tcgcttctga cgatgctcgg
     4141 catcatcatc ggcatcgcgt cggtggtttc cgtcgtcgca ttgggcaacg gttcgcagaa
     4201 aaaaatcctt gaagacatca gttcgatagg gacgaacacc atcagcatct tcccggggcg
     4261 cggcttcggc gacaggcgca gcggcaggat taaaaccctg accatagacg acgcaaaaat
     4321 catcgccaaa caaagctacg ttgcttccgc cacgcccatg acttcgagcg gcggcacgct
     4381 gacttaccgc aataccgacc tgaccgcttc tttgtacggc gtgggcgaac aatatttcga
     4441 cgtgcgcgga ctgaagctgg aaacggggcg gctgtttgac gaaaacgatg tgaaagaaga
     4501 cgcgcaggtc gtcgtcatcg accaaaatgt caaagacaaa ctctttgcgg actcggatcc
     4561 gttgggtaaa accattttgt tcaggaaacg ccccttgacc gtcatcggcg tgatgaaaaa
     4621 agacgaaaac gctttcggca attccgacgt gctgatgctt tggtcgccct atacgacggt
     4681 gatgcaccaa atcacaggcg agagccacac caactccatc accgtcaaaa tcaaagacaa
     4741 tgccaatacc caggttgccg aaaaagggct gaccgatctg ctcaaagcgc ggcacggcac
     4801 ggaagatttc ttcatgaaca acagcgacag catcaggcag atagtcgaaa gcaccaccgg
     4861 tacgatgaag ctgctgattt cctccatcgc cctgatttca ttggtagtcg gcggcatcgg
     4921 cgtgatgaac atcatgctgg tgtccgttac cgagcgcacc aaagaaatcg gcatacggat
     4981 ggcaatcggc gcgcggcgcg gcaatatttt gcagcagttt ttgattgagg cggtgttaat
     5041 ctgcgtcatc ggcggtttgg tcggcgtggg tttgtccgcc gccgtcagcc tcgtgttcaa
     5101 tcattttgta accgacttcc cgatggacat ttcggcggca tccgttatcg gggcggtcgc
     5161 ctgctcgacc ggaatcggca tcgcgttcgg ctttatgcct gccaataaag cagccaaact
     5221 caatccgata gacgcattgg cacaggattg aggttggaca aagatgccgc ctgaagctgc
     5281 agaaccggtc attttggagc agaaacttat tggacaaaaa cggtttctta gattctacgt
     5341 tccaaattcc cacttgcgtg ggaatgacgg cggcgggggt tcgatgattg cacacaacgc
     5401 tcgagtcccg tcattcccgt aaagacggga attcggttcg ttcggctttg cttgtttcgg
     5461 ataaatcacg gtaactcaat attccagatt cccgcccgcg tgggaacggc ggcggggctt
     5521 cgtattgttc aatttattat tttcaatcat tcaatgggtt aggatgtgtt tgttggcttg
     5581 ctaactttca gggcggattg gttttcaggc ggcatttccc gcaaaaaagg cttggaattt
     5641 tccaagcctt ttttgcggat ggattattga tttttgcgga tgatttcctc cagttggggc
     5701 atcgggctgt agccgctttg gctgcgcccg ttggggaaga cgagggtcgg cgtgccgttg
     5761 aagccgaatt gttcgcccaa ggaagtggtt tccgcgacgg gattgtcgca gatgctgccg
     5821 ccgaccggga atttgccttt acgcatccaa tccgtccacg ctttggcgcg gtcgggctga
     5881 caccataaga tttgcgcctt gcgcgcggca tcagggtgca ggccggcaat gggcatcata
     5941 aagctgtaaa ccgtcacgtc ggtcattttt tcaaactcgt gttccaagcg tttgcagaac
     6001 ggacaatcgg ggtcggagaa gacggcgact ttcagcttgc cgttgccgcg cacttctttg
     6061 atggctttgt ccaaaggcag ggaggcgaag tcgattttgt tcaaatcggc ggcgcgttct
     6121 tcggtcaggt ttttgcgcgt gtcgatgttg atgagttcgc cgacgaacat atagccgcct
     6181 tcggcatcgg tgtagataat ctgcctgccg ctgacgacga cttcgtaaat gcctttgacc
     6241 ggtgtttcgc tgacgctcaa cactttcaaa tcttgggcgg aataggtttt ttccaaacgc
     6301 gctttcaaag aggcggcaac ggatttgccg gcggacttgg ctttgacggc gggttcggcg
     6361 ttggcattgg aaacgggcgt ttgcccgcaa gccagcagcg ggaggacggt aaagggggtc
     6421 aagattttga ttaacttggt tttcatataa agatgattgc gcgtgttgga aaagcggaat
     6481 tgtatcaaat ctctgttgcg cctgcattgc gcctaggctc aatttatcgt ctgaaaatag
     6541 cttccgcctg ttaaaatacg caaaaaaatg atttgcctgc ctgtatgatc taccaccgca
     6601 tcgccgtaaa cgtgccgctt tcagacggcc ttttgactta ttcccattcc gatccgcttc
     6661 ctccgggaac gcgggtgctt gtgcctttcc gcaataaaac cgtggtcggg atggtgtggg
     6721 aaacggatat tgcgccggat atggatacgg cgcggatttt gagtattcag acgacctttt
     6781 tagacgagcc gccgttgcct gaaagctggc gtgatttgct ctcgtttacg tcgcgttatt
     6841 accactatcc gacggggcag gcggtgtttg ccgcgctgcc gcagggtttg aaggaaacgc
     6901 gcgcggtgga aatgccgcag ccgccgttgt tttatgcgtt gaacgaagcg ggcagggcgc
     6961 aaacgccgcc gccggcgcgg ttcaacaaaa aagcggcttt gtgggacgcg ctgctgtcgg
     7021 gcgggatgac gatggcggcg ttgaagcagg tgaacgcgca ggcggcgaaa ttaatcgaag
     7081 attgggcgga gcagggctgg attgaaacga cggaagcggc gaagcccgtt ttaaggtcgt
     7141 gtaatgggca ggcttcgcac tctgaatttg tattaaatgc tgaccagcag aaggcttccg
     7201 atgccattca gacggcattg ggacgcttcc agccgtttct gctgtacggc atcaccggca
     7261 gcggcaagac cgaagtgtat ttcgatgcga tggcgaaagt gttggctcag gggcggcagg
     7321 tgttgtttct gttgcccgaa atcaacctca cgccgcagct tttgaagcgg gtgggaaatc
     7381 gttttgccga cgtgccgacc gccgtgttgc acagtcagat ggcggcaggc aggcgcacgc
     7441 aggattattt gcgcgcgatg ttggggcagg cgaaattggt catcggcacg cggctggcgg
     7501 tgtttacgcc gatggatgat gtcgggctga ttgtagtcga tgaggaacac gacggctcgt
     7561 tcaaacaaga caacgaattg cgctaccacg cccgcgattt ggcggtgtgg cgggcgaagc
     7621 agggcggctg ccccatcata ttgggcagtg ccacgcccag cttggaaagc tggcacaagg
     7681 cgcaaagcgg cgcgtaccgc ctgctgcaac tgaccgaacg cgcccatacc gccgcgcaac
     7741 tgccgcaagt ggacattctc aacgtaggcc gtctgaaact cgacaacggt ttctcgccgc
     7801 aagccttgca gcttttgaaa cagaattttg aagcgggcgg tatgtcgctg gtgtacctca
     7861 accggcgcgg cttcgcaccc gcgctgtttt gcggcgactg cggccatacc ttcggctgcc
     7921 caaactgctc cgccaaaatg gtactgcacc aacgcgcccg ccaactgcgc tgtcaccact
     7981 gcgaccaccg cgaacccatc ccgtacaaat gccccgactg cggcaaccaa gacctgaccg
     8041 ccgtcggcca cggcacgcag cgcgtcgaag aaaccctgcg caccttcctg cccaaggcag
     8101 ccgtcgtccg tgttgacagg gacagcaccg cgcacaaaaa tgactgggcg gatttgtacc
     8161 gccgcatcgc cgacaacaaa atcgacattt tggtcggcac gcagatgctt gccaaaggcc
     8221 atgatttcgc gcggctcaac ctcgttatcg tattgaacgc cgacggcagc ctgtacagcg
     8281 cggactttcg cgcgccggaa aggctgtttg ccgagctgat gcaagtgtca ggcagggcgg
     8341 ggcgcgccga caaacccggc aaggtgttga tacagaccca actgcccgaa catcctgtct
     8401 tcgccgccgt caaagcgcaa gactatgccg tgtttgccga aaacgaattg aacgagcggc
     8461 aaatgttcgc catgccgccc ttcggtttcc agaccgccgt ccgcgccgat gcgccgcgcg
     8521 ttgccgatgc gatggaattt ctcaatgccg ccaaagaaac cctcgccccg ctgttgcccg
     8581 aaagcgtttc ccagttcggc gcggccccga tgctgatggt gcgcctcgcc gaacgcgaac
     8641 gcgcgcaaat cttcctcgaa tcgacatccc gacaggattt gcaccgtgcc gtgagtttgt
     8701 gggtgcaggt gttgcagcaa aaccgcgacg gcaaaatcag atggtcggtg gatgtcgatc
     8761 cgcaggaggc ttgattatcg gcaatacgat gccgtctgaa accgtttcag acggcatttt
     8821 tattccgggt cgcccgtaaa cgcattcgcc cgaaaaatcg gaacaaacgt gaaaagataa
     8881 agcgtgaaca cgacggcggt cagaatcgcg gggatggtga tgaagaatat cgggttcacg
     8941 ttcatcaaga aagtgcgcga gacggcggcg gcgaagagga tggggacggc aatgcggcag
     9001 agtttggggt agtcgagttt ggtaaagccg ctgtgccaca gtccggcggt cagccacacc
     9061 atcatcacgc tgcccatcat gccaccgagg gtaatcaggt gcaggggcgc ggaggcgggc
     9121 aggttttgta atttcgccgc gcctgtccac aaatagcctg cggcggcaaa gagttggagc
     9181 aggtaataag tgcggacgta gtgtttgcgc aggagttcgt gatggtgaag ctcacgcagc
     9241 ttggcaagca ggataaagcc gacggcgagc gaggtaaaac cggcggtttg cgcaggcagc
     9301 caaagttcgg cggcggcgtg caggagcagg aaggtaatgg cgatgttttt atagacgaca
     9361 ttggggatga atactgggtc tttcagacgg cattctttca gggcttccgc gcccaaaaga
     9421 atactgacgc gcacggatac gaacatcacc gccgccatat ttagatgcac ttgcgcgcgc
     9481 aacaggttca aatcgccgct gacggcatat gccgtctgaa aaacagtgaa cgcggcaagt
     9541 aacattagca gggcgaagtt gtcggtgttt cggtctagcc aaatcagccg ggcgcagaac
     9601 agcagcaaca ccagccaata ggcggcgacg aaaaacgag
//
LOCUS       DAWHXZ010000061         9614 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000061 DAWHXZ010000000
VERSION     DAWHXZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.061"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..293)
                     /locus_tag="WIE35_000938"
     CDS             complement(<1..293)
                     /locus_tag="WIE35_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ4153869.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNR"
     gene            complement(614..1207)
                     /gene="ribA"
                     /locus_tag="WIE35_000939"
     CDS             complement(614..1207)
                     /gene="ribA"
                     /locus_tag="WIE35_000939"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ4153870.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(1200..1874)
                     /locus_tag="WIE35_000940"
     CDS             complement(1200..1874)
                     /locus_tag="WIE35_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153871.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMGMGTASIDRSV
                     LWRSDEGWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENQAFQ
                     LICLSQQGEGLENIWNLDENVADVSLACRCAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPICTESGGVLGVVHVEFECAECADEAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            2028..2112
                     /locus_tag="WIE35_000941"
     tRNA            2028..2112
                     /locus_tag="WIE35_000941"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2062..2064,aa:Leu,seq:tag)
     gene            complement(2203..2391)
                     /locus_tag="WIE35_000942"
     CDS             complement(2203..2391)
                     /locus_tag="WIE35_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153872.1"
                     /translation="MADERKGFFDAIIKINIFFFPVETPALGAVNLIAGGSTRFRFGA
                     EQMAALYVPFCVLKHIGR"
     gene            2632..3666
                     /gene="purM"
                     /locus_tag="WIE35_000943"
     CDS             2632..3666
                     /gene="purM"
                     /locus_tag="WIE35_000943"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ4153873.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
     gene            complement(4140..4796)
                     /locus_tag="WIE35_000944"
     CDS             complement(4140..4796)
                     /locus_tag="WIE35_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ4153874.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECIRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPMLE
                     "
     gene            complement(4793..6565)
                     /locus_tag="WIE35_000945"
     CDS             complement(4793..6565)
                     /locus_tag="WIE35_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ4153875.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLKRIAQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQPQQGTTVSLTGASEESLK"
     gene            6771..7385
                     /locus_tag="WIE35_000946"
     CDS             6771..7385
                     /locus_tag="WIE35_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ4153876.1"
                     /translation="MKTSNFSKRTSAMKTFALILAGGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARSVRFEFDGHFADLNTQTDLQEG"
     gene            7548..8165
                     /locus_tag="WIE35_000947"
     CDS             7548..8165
                     /locus_tag="WIE35_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ4153877.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKSNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            8173..9036
                     /locus_tag="WIE35_000948"
     CDS             8173..9036
                     /locus_tag="WIE35_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ4153878.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYANKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(9049..9483)
                     /locus_tag="WIE35_000949"
     CDS             complement(9049..9483)
                     /locus_tag="WIE35_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ4153879.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
BASE COUNT         2376 a         2334 c         2570 g         2334 t
ORIGIN      
        1 cggttggtat cggcccaata gtcgtcgctg tccaaaaaga ttaggtaatc gccttttgcc
       61 gcccggatac cggcgttgcg ggcatccgac agcccgccgt tttcttgatg aatcactttt
      121 atatgcggat atttgcctgc atattcgtcg caaatcttcc cgcagccgtc cggcgaaccg
      181 tcatcgacca aaatcatttc ataatcggca aaattttcgg caagcacgga atccacgcag
      241 cagcggaggt atttttccac attgtaaata gggacgatga tggagaaaat cataaatatc
      301 aatccattat attaagatgt ttgcgcgtat gcctcaaacc cgcgctcgca atgcgtttgc
      361 atccgcaccc tgtaacttta tatagtggat taacaaaaat caggacaagg cgacgaagcc
      421 gcagacagta caaatagtac ggaaccgatt cacttggtgc ttcagcacct tagagaatcg
      481 ttctctttga gctaaggcga ggcaacgctg tactggtttt tgttaatcca ctataaaact
      541 tattgcttta tcaaggtatg gaaacctgtt tcccgaaagg cggcgcagga tgcccgttcc
      601 ctgcaacttt gccttattcc gacatcagat gtcccagctt gtctgctttg gtttggaggt
      661 agcgtttgtt ttcaaggttt tccccgacgt gcagggggat gcgttcgacc acgttaatcc
      721 ccgcatcttt cagggtttgg attttttcgg ggttgttggt cagcagtttg accgagcgga
      781 tgcccagata ttcgtagata gattgcgcca aacggaaatc gcgggcatcg acgggcagcc
      841 cgagtgccaa attggcttca accgtatcca taccttggtc ttgcagatga taggcgcgga
      901 ttttgttaat cagcccgatg ccgcgtcctt cctgacgcag atagacgatg atgccgcgcc
      961 cctctgtctg taccgccctc atggccgctt caagttgagg tccgcagtcg cattttctcg
     1021 agaacagcgc atcgcccgtc aggcattcgg agtggatgcg cgtcagaacc ggattgccgt
     1081 ctgaaaaatc accgacggtc agcgcgacgt gttcctgccc gtttgcctct tcaaagccgt
     1141 gcatcgtaaa tacgccccat tcggtcggca ggcggcagga agcaacatag tttaataatt
     1201 cagacatttt catctccttc tgcggcagtg atgcccaaaa gctgcttcaa aggttcggac
     1261 aaagccaagg caagagccac ccattccgcc tgcgctgcct catccgcaca ctctgcgcat
     1321 tcaaattcca catgaaccac gcccaacaca ccgccacttt ccgtgcagat tggaatggaa
     1381 atttgcgccg ccgaagcatg attgcgttct cccgaaagct cccccaaatc caaccaacgg
     1441 cgtacatccg aggcaacatt catccagccg ctttgcgcgc aacggcaggc aagtgaaaca
     1501 tcggcaacat tttcatctag attccatatg ttttccaaac cttctccctg ttgggacagg
     1561 catatcagtt ggaaagcctg gttttcagac ggcatcaagg catagaccgc cgcactccgc
     1621 acgcctgtcg agcgcgaaaa caccgaatcc aaagccatga aaagccgttt cagtgcatct
     1681 tcgcggacat tgtcgcacga caggtaatcg gcaagtttcc aaccctcatc actgcgccac
     1741 aaaaccgaac ggtctatcga agccgtcccc atacccatta ccgtctgcgc cgtcagatac
     1801 gccgcccgaa cctcgtcaag cggcagcttc aaaccctgag tcagcagaaa atccttaatc
     1861 aaagcagcag gcatacccaa attccgtcaa taaaaacaaa aaaccgcccg attccgatgt
     1921 caggcggacg aaaaacagat tttaccgccc caaaccccaa gccatcaaca gccccgccga
     1981 aaaccttgca caaaaaaaca aaatctgtat aatcgtccaa ttcccatgcg gacgtggcga
     2041 aattggtaga cgcaccagat ttaggttctg gcgccgagag gtgtgagagt tcgagtctct
     2101 ccgtccgcac caataatgat aaataaatta tatagttaat gcttatcggg acaaattagt
     2161 gccacaaaac ataagtcgtc tgcttttcag cgggcggctt ttttatctgc ctatatgttt
     2221 caacacgcag aacggtacat aaagtgccgc catttgttcc gcaccgaatc ggaaacgggt
     2281 gcttccgccc gcaatcagat tcaccgcccc taatgccggg gtttcaaccg gaaagaagaa
     2341 aatattgatt ttgatgatgg catcaaaaaa ccccttccgc tcgtctgcca aaaggaatgg
     2401 ttaagaaaag caatgcgtct tttgatggaa cgcgatatat aaggaattga ggggaagggc
     2461 aggcgggttt ctgcccgtta atcctgcgga cggtcccgcc aaacccgaca cgcgcgggta
     2521 tatggcggac tgtttacaat attgtctaaa aatctgaacc tgcaaacgtg aaataaggta
     2581 gaatacgccc cgttatttta ccgtccaatc cccatttacc aaggaaaaac gatgagtact
     2641 tcattgagtt accgcgatgc aggtgtcgat atcgacgcag gcgaccaact ggtcgaaaac
     2701 atcaaaccgt ttgccaaacg caccatgcgt ccggaagtat tgggggattt gggcggtttt
     2761 ggcgcattgg tcgaaatcgg caagaaatat aaaaatcccg tattggtcag cggtacggac
     2821 ggcgtgggta ccaagctcaa acttgccttc gattgggata aacacgatac ggtgggcatc
     2881 gaccttgttg caatgagtgt caacgatatt ttggttcaag gagcagaacc tctgtttttc
     2941 ttggattatt tcgcctgcgg caaattggat gttccgcgcg caaccgatgt catcaaaggc
     3001 attgcacaag gttgcgaaga atccggctgc gccctgattg gcggggaaac tgccgaaatg
     3061 ccgggtatgt atcccgtcgg agaatacgat ttggcgggtt ttgccgtcgg cgtagtggaa
     3121 aaagagaatg tcattaccgg ccgcagcatc ggcgcgggcg atgtggtatt gggtttggct
     3181 tccaacggcg cacattcaaa cggctattcc cttatccgta aaatcatcga acgcgacaat
     3241 cccgatttgg atgccgagtt tgataatggc aaaaccttgc gtgaggctgt tattgcgccg
     3301 acccgtctgt atgtgaaacc gattcttgcc gctttggaaa aatttaccat taaaggtatg
     3361 gcacacatta ccggcggcgg cattaccgaa aacgtgccgc gcgtgttgcc tgaaaacacg
     3421 gttgcacaaa tcgatgctaa atcgtgggaa ttgcccaagc tcttccaatg gcttcaaaag
     3481 gcgggcaatg tggaaaccca agaaatgtac cgaaccttta actgcggcat cggcatggtc
     3541 gttattgttg ctgccgaaga tgccgatgcg gttcagggtc tcttgggtga acaaggtgaa
     3601 acagtttacc gtttaggttt gatccgtgag cgtcagggag acgagcatca aacccaggtt
     3661 gcctgattgc ttctatagcg aaatgccgtc tcaaacatgg gtttggacgg catttttata
     3721 gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaga tagtacggca
     3781 aggcgaggca acgccgtact ggtttttgtt aatccactat atattgattg atatgagggt
     3841 gggttacaca tgcttaaacg atataagcca ttgtatattt atgggattcg gaatggaaca
     3901 ttttaccctg cattagtatt tccgcttttt attatgccgt ctgaaaatat catgatgatg
     3961 cgttttcaga cggcatagct gatattgcag tatcagatag gggcaaacgg aaaacactga
     4021 accgaacaat attactgcta tgccaagcgt acttatgggg gcataaactt acatgcttgg
     4081 tctgttatcc gaatgaccgt acggggatta aaacccctaa tatgccgtct gaacccgcct
     4141 tattccagca tgggttgggg gacgttgtgc cggatggcgt aaacagcggc ctgcacccgg
     4201 ctgctgaggt tgagtttgcg gagcaggttt tgaacgtgga ctttgacggt ggattcggca
     4261 agatcgaggt ggcgggcgat gattttgttg ctgtgccctg cggcgagata gcccaagatt
     4321 tccagttcgc gaggggtaag tgaggagagt gcctgtgtcc cttgggcagg ttggggggaa
     4381 atcaggcttt taacgagttt ggcggtcatc tcgggcgaga atacattatc gccttcggcg
     4441 gctttgcgta tgctttcgag cagaaagtca gcgttgatgt ttttcagcag gtaaccgcgc
     4501 gcgccgatgc gaatacattc ggtcaagtcg tcgctgtctt cggaaacggt cagcataatc
     4561 actgcctgct gcggattgat gctgatgatt tgggagagtg cttcgcgtcc gttcatgccg
     4621 ggcatatcca aatcgagcag gacgacatcg ggttgcagcc gactaatcat tttgatgccc
     4681 gagaggccgt ctgcggcttc gccgatgact tcaaaaccgt gttggcgcga caaaagggct
     4741 ttaatgccgc tgcggaagag ggtgtggtcg tctatcagaa taattttaat ggtcatttca
     4801 agctttcttc agatgcaccc gtcaatgaga cggtggttcc ctgttggggt tgggaacgga
     4861 tttctaaaac ggcatggatg cgcttggcac gctcctgcat gatgtgcagt ccgacatggc
     4921 tgcccgtagg ttctcctatt ttctccgtgt cgaaaccttg tccgttgtct tggatggtca
     4981 tggtaaagcg tccgccgtgt tcggaaaggg tgaattttac atgggtggcg cgggcgtgtt
     5041 tgcggatgtt ggacaggctt tcctgcagga taaaaatcat ttggagctgc gcttcctgag
     5101 gcggcaggaa cgaaccgttt tcccaggcgg tttcgaccgt tatcccggtt tgttgcgtaa
     5161 agcgggcgaa taggtcggca acggcttcgg gaaattcttt attgctgatt ttggtacgga
     5221 agttgagcag cagttcgcgg acatcttcat aacattcctg cacgcctgtt ttgatgaagc
     5281 cgatgttttc tgcggcttcc tcccgtttgt tttcggcaaa ggcggtttcc agcatctgta
     5341 cctgtaggtt taggaacgtt aatgcttgtg cgatgctgtc atgtaatcct tgcgcaatca
     5401 ggttgcgttc ctgcaatact gcaagcaggc gtttttcttc ctcctgtttt gcgccggcaa
     5461 gcgatacgcc caattgcctg cctagtgttt gaagcaggat gcggtcgtct tcatcaagag
     5521 aaatgccgtt tggaaagctg agcaacagcc tgcccaatgt ttcgttctgg tactcaatgg
     5581 ggaagatttc ctcatggtac ttccccaaat ccgaagctgc tgtgccgcaa tccgcatgat
     5641 gaatggaaac ataaacatcg gatccgccgt ccaaacaaac tctgccggaa tctgctccta
     5701 cggcgggcag gatacggttt agaaaatgtt ctgcagcctg ttgcggtatg taggattggt
     5761 gcaggtcccg tgtagtttgg tacagcaggg tcaggttttg attttgtttt tcgagactgc
     5821 gtgtctgctc ggcgacttgt ccttccaaat catcatataa aattttcaac ctgccgccca
     5881 tttgattgaa acaacgcccg acctgtttga attccggcgt accgccttcg ggaaccggaa
     5941 tatcgaaaca cctccgtccg atgcgttccg caccttccct taacgcctgc agcggccgga
     6001 taacccaaat ctggtgccaa aacagcatca gtacagacga caccagcgtc atcaacataa
     6061 ttgcccattg aaaacgcctg agccaccatg tgtttttttc gttggcattt tccaatgcct
     6121 gcaaaaacag ttcgatgttt ccggcaaagc ggtagagatc gacctgagtc ggtcgccggt
     6181 aggactggag cggggggagg atgtgtgcct gccaatctat aatcagcatg gattgtatca
     6241 aatcataagc aagaggggtg tccgaaggaa tcagcggatg gatggcatcg ctttgggcaa
     6301 tgcgttttaa acttttttca aattcggcaa cctgattgtc aatttgcgca cggggcgagc
     6361 cttcacccgc catgtatgcc agacggtatg cctgcattct caagttgccc gcctcttcga
     6421 tgacggaggc cgcgttttcc agacgcaaag agagcagcag tgtcaaaacg acagacaatg
     6481 ccgccaaccc gacccacagt ccggtcagga gtttcaggcg cagggaaagg ctgatgccgt
     6541 ctgaaaaacg ggctggcagt atcatgctcg gcgaaaaatt gttctaaata atgcaaacaa
     6601 tatcattctt tggaattaga tacaactgct cagaaagaat tggttaagga aaacttaatc
     6661 cgcaccgctt cagtgttaaa tcatgttatg ataaaattca gataatatga atttatttgc
     6721 cgtctgcttc agacggcatt ttgagtcatt gggaacggca gactgcaatc atgaaaacct
     6781 cgaacttttc caagagaaca tccgcaatga aaacttttgc cctgatactg gccggcggtc
     6841 aggcgagccg tatgggaggc gaggacaagg ggcttgctct tttggggggt aaggcactga
     6901 tagaccatgt catcgacagg gtcaggccgc aggtcagcca tatcgccatc agcaccaacc
     6961 ggaatttgga agaatatgct cgaagaagtc cgcatatttt tcccgatgcg cggcagtggc
     7021 agcatttcgg cccgctttcg gcattgtgta ccgcagccaa cgatttgcag ttggcgactg
     7081 ctgactggct tttggttgtg ccgtgcgata tgccgtatct gccggacgat ttggtggcga
     7141 ggtttgaaac cgtgtcgaaa cgcacaccgt tgtgcaatgc gtattatgtg gaaacgccga
     7201 taacgatgca ctacaacatt atgtatatcc gcccgcagat tctgcaaagc gcgattccct
     7261 atctgttttc gggtatgaaa acattaagaa gctggttgca gcagcaaagg gcgcggtcgg
     7321 tcagattcga gtttgacggg cattttgccg acttgaacac gcaaaccgat ttgcaggagg
     7381 gataagggca agaccgccga ccggcgtgga aaggaaaggt caagccatac cgggcggttt
     7441 ttgcccgaat cggaggcata atgctgtctg aaggcatttc agacggcatt tttcgcgggg
     7501 agatgctaca atttgcacca tttttaccga cacagggaaa caggattatg tttacaggca
     7561 ttgttcaagg attgggaaaa ctgacggcaa tccaccgccc gtcggaggca tttcagactt
     7621 atgtcgtcga gcttccgcaa gaggcggcgg acaatctgca acgcggcgca tcggttgcca
     7681 ataacggctg ctgcctgacg attaccgaaa tcgaaggaaa ccgcgtcagc ttcgatttaa
     7741 tggcggaaac tttggcaaaa agcaatttgg ggctgctgaa ggaaggcgat tgcgtcaaca
     7801 tcgaacgggc ggcgcgtttc ggagacgaaa tcggcggaca cgtcatgagc ggacacatta
     7861 tggcaaccgt gcctattgtc gaaatcgaac gggacgggtt caaccgcacg gtttggtttt
     7921 cgctcccgca tgaactcaaa ccctatatcc tgaccaaagg gttcgtcggc ttggacggtt
     7981 gcagcctgac cataggcaaa gtcgaagacg gccgtttcaa tgtccatctg attcccgaaa
     8041 ctttggaacg gacgctgttc ggcagcagaa aggtcggcga caggatcaac atcgaaatcg
     8101 atccgaatac gcaggcaatc gtcgacaccg tcgaacggct gatggcgcaa agatatgcaa
     8161 agtgaggtag agatggaatt gaacgaattt ctcgataaag cctatgccgt tttgcggcgt
     8221 ttggatgccg tgcttccgcc cgaacccggg catacggatt ggaacgcgct tgccttccgc
     8281 tggcagagtg cgggcaaaaa aggttttttg gaacacttgc ccgatccgca cacctttccc
     8341 ttggtaaggc tggcgggagt cggcaggcaa accgaattgc tggtgcgtaa taccgaacag
     8401 ttcattgtcg gcagacccgc gaataacgta ttgatgagcg gcgcgcgcgg aacaggcaaa
     8461 tcctcgctgg tcaaagccct gctacacgaa tatgcgaata agggattgcg cctgatcgaa
     8521 gtcgataaaa gcgatttaat cggcctgcct tacctgttga cgcttttgaa ggaacgtccg
     8581 gaaaaattta tcgtattttg cgacgatttg tcgtttgaaa gcggcgatga aacctataag
     8641 gcattgaaaa ccgcgttaga cggcggtctg tctcaacgtt gcgccaacgt tttggtttac
     8701 gcgacttcca acagacggca tctgatgccc gaatattttg acgagaatgc cggtacgacg
     8761 gggatgcggg gggaaatcca tcagaaagaa gcggtggaag aaaaaatatc cctgtccgac
     8821 cgattcggat tgtggctcag tttttatccg ttcgatcaaa acgattatct ggcggcggtg
     8881 caaaattggc tggaagattt cggcgtgccg tatgatgaaa ccgcacagat ggcggcattg
     8941 cagtgggcgc agacgcgggg cagccgttcg ggacgttccg cgtggcagtt tgcctgcgac
     9001 tgggcgggca ggctgccgaa acaacgggcg ttgtgagcgt ttcggggatt atttgaggca
     9061 ggaagaaagc cacatttgct gctgttgcgg tgtgaggata tggaagaacc ggtgttggat
     9121 ttccaattcg tccaccgcaa aatccatacc ggacaaatag cggctttcga cataatcgcg
     9181 cgcctcgttc cgattaaaaa catccgagga aatgatttcg acgacagacc ggcggcggct
     9241 gtgttcggaa tgcataacct tcaaacgcgc cctgtcgccc gccattttga aggcggcgcg
     9301 gattttacgc agttcattgt gctgaccctg tgtcagtccg agccggcgta tgtcgcagtt
     9361 cggttgaaaa tcgttcaggg ggatataagc cgtggcggcg tgggaaagct ggcaggaaag
     9421 caaagccata cttaaaaaag aggcggcagg tttggcaaaa cggcagggag caggcagtgg
     9481 cacggctttt cctcttgttg gaaattttag gaaatatact agattgacgt gtgaaagatg
     9541 gtattatcgc ggttaacttt tgttaataat gcgtacgtat gtccggcagg cagacataac
     9601 ggcggaatcg gaac
//
LOCUS       DAWHXZ010000062         9504 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000062 DAWHXZ010000000
VERSION     DAWHXZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.062"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(193..1116)
                     /gene="speB"
                     /locus_tag="WIE35_000950"
     CDS             complement(193..1116)
                     /gene="speB"
                     /locus_tag="WIE35_000950"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ4153880.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGIIDPAHSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(1215..3107)
                     /gene="speA"
                     /locus_tag="WIE35_000951"
     CDS             complement(1215..3107)
                     /gene="speA"
                     /locus_tag="WIE35_000951"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ4153881.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(3274..3558)
                     /locus_tag="WIE35_000952"
     CDS             complement(3274..3558)
                     /locus_tag="WIE35_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ4153882.1"
                     /translation="MKKLLLAAVVSLSAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(3709..5517)
                     /gene="aspS"
                     /locus_tag="WIE35_000953"
     CDS             complement(3709..5517)
                     /gene="aspS"
                     /locus_tag="WIE35_000953"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ4153883.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGAENGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(5575..6276)
                     /locus_tag="WIE35_000954"
     CDS             complement(5575..6276)
                     /locus_tag="WIE35_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ4153884.1"
                     /translation="MTEPEAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(6419..7567)
                     /locus_tag="WIE35_000955"
     CDS             complement(6419..7567)
                     /locus_tag="WIE35_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ4153885.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(7728..7991)
                     /gene="rpsT"
                     /locus_tag="WIE35_000956"
     CDS             complement(7728..7991)
                     /gene="rpsT"
                     /locus_tag="WIE35_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ4153886.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            8294..9436
                     /locus_tag="WIE35_000957"
     CDS             8294..9436
                     /locus_tag="WIE35_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4153887.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         2289 a         2458 c         2408 g         2349 t
ORIGIN      
        1 cctccgtaaa aaagaccgtc ccgaaaaatc ggaaaaataa tatttaacta attgttttat
       61 aagatatatt ccgatatttc accgtctttc cgatatgcgg ctccgggcaa cttttgtttc
      121 agtatttgaa ttttcattag actgaatacg ccgtttgaac ggcacggcga aaacccgggg
      181 gatgccggac gttcagtcct ttttcgcacc ttgaaggtaa agcatttcca aggcaattgt
      241 ggcaccggcc aaagcggtaa tgtcggattg gtcgtaagag ggggcaactt ctacaacatc
      301 cataccgacg atgtcgagat ccgtcagccc acgtaggatt tttaatgccc tgtcgctgct
      361 caagccgccg catacgggcg taccggtccc aggggcgaac gacgggtcca ggcagtcgat
      421 gtcgaaagtc aggtaaacgg gcatattgcc gacggtttct ttgattttac ggacggtctc
      481 ttcaacactg tcttcattga cttggggggc ggtcaacaca gtaaaaggca attttttact
      541 gtgttcggta cgtatgccga tttgcacgga atgtgccgga tcgatgatgc cttccttggg
      601 ggcggtataa aacatcgtac cgtggtcgta ttcgctgccg ttgtcgtagg tgtcggtgtg
      661 cgcgtcaaaa tgaatcagtg cgagtttgcc gaaatagcgg gcgtgggcgc gcaacaacgg
      721 tagggtaatg aaatggtcgc cgcccaaact caaacagcgt ttgccggaag aaagtaattt
      781 gccggcgtgc gcttccattt tttcgacaaa atccctgctg tcgccaaaag aaaaaaccaa
      841 gtcgccgcaa tcaataatgt tcaggcgttc gcgcacatca aatgtccatg gaaacctgcg
      901 gtgctcccaa gcgaggttga cggaggcgcg ccggatggct tcaggaccga aacgcgcgcc
      961 ggaacgccct gaaaccgcca tatcataagg cacgccggta ataacccaat cggcatgact
     1021 ttcatacggc ataaaattaa gcggcaggcg caaaaacccg aaattattgg aaacgaggga
     1081 gttgtcggtt tgtcctgcca gtgtgctgta ttgcatcgta atgattcctt gtaattggtt
     1141 tcaatcggtc gtgatgattg gtgtttgagt aagaaaatcg ggcttcagac gacatatccg
     1201 atgccttgat gcgtctattc gtcttccaaa taggtataac cattaagccc cgcttcgagt
     1261 tcttttaaga aagacatagc ctgcgaggca ggaaggtctg aatgttcgat ttgttcgcga
     1321 tagcgtttca tcagctcttt cggatcttga taaacgtatt cgagcatatc ggcaacggtg
     1381 tttccttcat cgtaatcgat gacggtaaat tgtccgtctt cccctacaac aacatcggca
     1441 gtggcagtgt cgccgaaaag attgtgcata ttgccgagta tttcctgata tgctcccacc
     1501 ataaaaaagc ctaaaagcgg cggctcttct tcgggataat caggcatagg catcgtaccg
     1561 gcgatgccgt cgccgtcgat gtagtggtca atcgtaccgt ctgaatcgca ggtaatgtcc
     1621 aacaacacgg cgcggcgcgc aatcggttca ttcaaaccgg taatgggaca aacagggaaa
     1681 agttgatcta tgccccaagc atcgggcaaa gattggaaga gtgagaaatt gacatacagc
     1741 ttatcggcaa aacgttcttg caattcgtca ataatggttc ggtgagaccg gtgtttttca
     1801 ttaaacaatt cgccgacttc atgacagata tttaaataca gttgctccgc ccacgcacgt
     1861 tgcgccaaac tcaacagccc gacattatac tgattatgca catcggcaag atcaaactgc
     1921 ccttcgtgta tccagctgcg taaggaacgt ttttcccgcg aggcggaaat atccgtccaa
     1981 gtttcccaca tactgtgcaa cacacgcggt gcttcgggcg atggcgcatc cagccggcgc
     2041 ggtttgtaac gttcaacgcc tataacatta gcaaccaaaa cggcgtgatg tgcggtaatg
     2101 ccgcgcccgc tctcggtaat gattgtcgga tgcggcagcc cgtgttcgag acaagcctgg
     2161 ctgatgcccc atacgactgt ggcggcatat tcgttgaggc tgtaattaac ggaacaatcc
     2221 gattgtgtgc ggtttccttc gtaatccacg ccaagcccgc cgcctacatc aaaacagcgg
     2281 atatttaccc ccagtttgtg caactcaaca taaaaccgag ccgattcgtg tacacctgtg
     2341 gcaacatcac ggatgttccc aagctgcgag cccaaatgga aatgcaaaag ctgcaggcaa
     2401 tccagcctgt ttttttgttt caaaatatcg accagttgca aaacttggga agccgacaag
     2461 ccgaattttg atttttcccc acccgaagac tgccattttc ccgaaccttg ggaagccagt
     2521 ctggcgcgca cacccaaacg gggcttgatg ccgagttttt ccgcctcttc caataccatt
     2581 tgtatttcgg acagcttctc aatcaccaaa taaacctgat gccccagttt ttcgcccatc
     2641 aaggcgaaac ggatatattc acggtcttta tagccgttgc agacgattaa tgtttgccgg
     2701 gtgccggcgt gtgccaaaac cgccatcagt tcggctttag aaccagcttc caaaccatgc
     2761 ggttgtccgc ttgacataag cgattcgatg acgcggcggt gttggttgac cttgataggg
     2821 taaaccaaac aataaccgcc cttatagccg cactcttccc gtgccgtctg aaaggcgcgg
     2881 ttgatgtcgc ggaggcggtg ttcgaggatt tgcggaaaac aaaacaaaac aggcaggcgc
     2941 gcctgatgtt tttgttgcac ggcttcagtc agtttttgca gtgaaacagt ttgattgtgt
     3001 tgcgagggat tggggcggac gatgatttcg ccggaatcgt caacatcata ataacctata
     3061 ccccaatgat taatgttgca cacttcacgg atggtaagga taggcataat aaacctgctc
     3121 cgtctgtcgt gttgaaaagg aatgattata acaaatcagc gtgaaatgtc atttttttaa
     3181 taagaaaagc ctgcctcata cctgatgagg aacaggcaaa atgccgtctg aacgcttcag
     3241 acggcatttt ggttcagctt gccatcaaag gagtcagcga tcgagctgct ctttgatgat
     3301 tttcaggtcg gggtaagaca acacgatgtc gtattcgcgg ccgtctttat aggcttccac
     3361 ttccagcaca ggcttacccc aatggtcgtc ggcatcgaca tcgtgaacct gataaccacg
     3421 ctgttccaac attttcacgg cttttgtgcg gttttgttca aaatggggat cgccgtaaat
     3481 ctgacgctcg gcagaatcgc cggcaaatgc ggttgcggca ctcagggaaa caacggcggc
     3541 caataacagt tttttcattt tcagtccttt ttttatcggt tgattgaaca agatgtgttt
     3601 ttcaataccg ccattaaaac acagcaaaat taggtttgaa ttagagggaa gtcaagaagc
     3661 gtaaatgccg tctgaaaaaa acagagccgt caaacggctc tgttttcctt atgcttcttt
     3721 ggtttcagcc gccttttgac gcaggcgcaa gctcaattcg cgcaactgct tgtcgtccac
     3781 gctgttgggc gcgttggtca gcaggcattg agcacgttgt gttttcggga aggcaatcac
     3841 gtcgcggatg gattcggctc cggtcatcag cgttaccaga cggtcgaggc cgaatgcaag
     3901 gccgccgtga ggaggcgcac cgaatttcag gttgtccaag aggaagccga atttctcttg
     3961 ttgctcttca gggctgattt tcagcgcggc aaacactttc tcttgcacgt ctgcgcggtg
     4021 gatacggata gagccgccgc cgatttccca gccgttcaat accatatcgt aggcgcgtgc
     4081 caaacaattt gccgggtcgg aaaccatcag gtcttcatga ccttcttttg gcgcagtaaa
     4141 cggatggtgt acggcaacgt agcggtcggc ttcttcgtcg tattcgaaca ttgggaagtc
     4201 gacaacccac aaaggtttcc attcgtctgt gaaatagccg ttttccgcgc cgtgttccaa
     4261 gccgactttg atacgcaatg cgccgatggc ttcgtttacg actttggttt tgtctgcacc
     4321 gaagaagata atatcgccgt tttgtgcgcc ggtacgcgcg atgatttcct tcagggcatt
     4381 ctcagacagg aattttacga ttggagattg caggccgctg tcttcgccgt tggagaggtt
     4441 gcccacatca tttactttga tgtatgccag acctttcgca ccgtagatgc cgacaaattt
     4501 ggtgtattcg tcgatttctt tgcggctgaa tttcgcgccg ttcggtacac gcagagcgac
     4561 tacgcggccg cctttcatat ctgccgcgcc acggaagact ttgaattctt ccgttttcat
     4621 caggtcggtc aactcggtga atttcaagtt gatacgcatg tccggtttgt cagagccgta
     4681 gtagaacatg gcttcagagt aaggcatgcg tgggaagtcg cccaagtcca cgcccagcgc
     4741 gtctttgaag acttgtttcg ccataccttc ggtgatgtcc atgatttcat cctcgtttaa
     4801 gaacgaggtt tccaagtcga tttgggtaaa ttcaggctga cggtcggcac gcaggtcttc
     4861 gtcgcggaag cacttggtga tttggtagta acggtcgaaa cccgccacca tcaacagttg
     4921 tttgaataat tgcggcgatt gcggtagcgc gaaaaactcg cccggatgaa cgcggctcgg
     4981 cacgaggtag tcgcgcgcgc cttcaggcgt ggagcgggtc agcatcgggg tttcaatgtc
     5041 gatgaaacct tgcgcgtcca agtagcggcg aacgcccata gccacttggt aacgcaggcg
     5101 caggttgcgc tgcatcaccg gacggcgcag gtcgataacg cggttggtca ggcgaacgtt
     5161 ttcgctgatg ttttcatcgt cgatttggaa cggaggcgta gcggcggcgt ttaatacttc
     5221 gatttctttg gcaagaattt cgattttgcc ggaaatcatt ttgtcgttgg ttgtaccttc
     5281 gggacggttg cgtacgcggc cggtaatgct caaaacgtat tcgttgcggg cggaatcggc
     5341 agcagcaaac gcttccggag tgtcggggtc aatcacgact tggacgatgc cttcgcggtc
     5401 gcgcaggtcg ataaaaatca caccgccgtg gtcgcgtcgg cggtgtaccc agcctttgac
     5461 ggttacggtt tggtctaagt attgctcgct gatcaggccg caatagttgg tacgcataaa
     5521 ttcacctttt attgatttaa actgaaaaca gaaaatgccg tctgaacggc ggctttattg
     5581 ttgttcgggc aaatccgcct tttcagacgg cataggtcct gccaatgttt tgacgggcag
     5641 gtcgtcaggg atgaccatac ccagcgaaat cacatatttc aatgcttcgt ccacgctcat
     5701 atcgagttcg cgcacatcgc ttttctttac cataatatag taaccgccgg tcggattcgg
     5761 cgtggtcgga acatacacgg aaagataatc gccgtccttc ggcaatgcgg ccttaaccgc
     5821 attcgacacc tgaccggaca cgaatgcgat tgtccaaata cccgattggg gaaacggcac
     5881 gagtactggt gttttaaacg aacggctgct gtcggacagc agcgattcgg atactttttt
     5941 cacactcgaa tagatggact tcacaactgg aatccgcccc aacaggctgt cccacgcggc
     6001 aagaatctgc cggcccaata cgtttgcggc aaacaatccg gttacaaaca atacagcaat
     6061 ggcaacgata acgcccagcc ccgggatatt gaagcccaaa acatattgcg gccgccattg
     6121 cttcggcagc aggttgacga gctgatcgga cgcggaaacg atataggaaa ccacccaaac
     6181 cgttaccgca atcggcagcc agaccaaaat gcccgtaatc agatattttt ttaacgcctt
     6241 ggcagcttta ccgccttcgg cctcaggttc cgtcatcttg cttgattccg acaaagtccg
     6301 tacaaaccgc acattatacg cgtttgcccg gattcaaacg aaatttttat cccgccccgc
     6361 caaaccgcca gcggcttcag acggcacggc aacttgatat accgtctgaa cacgcggttc
     6421 agatgccgtc caagtcgttg aacatcaacc cgataccgat accgttctgc ttgtggttgt
     6481 agtcgatcag gctctcgccg taaccgtgga atccgcgtac cacgcctttg agtttgccct
     6541 taatcggaaa cgtgtaggcg gcttcaatcg cgccgtagcc cgttttgggg ttgtagcgca
     6601 atacggaata cacattctgc ctgtcgttca ggcggtactg cagcttcacg tcgccatacc
     6661 ccatatagtc ggcaatatcg ggattgtcgt ttttatcgcc gctctgatcg aacgcacgca
     6721 cccacacgcg cggaatcacc gtcaatttgc cccattccat gcctgccatg gcgtaaatcc
     6781 tgttccacga acgcgattcg ggacggctct gtccgttgga ctggtggaca aaacccgcac
     6841 cgagcatacg cagcctgccg ccgaacggca aatccgcctt cacaggctgg gtcaggaaaa
     6901 tttcaggttt gtaatccgta ttgcggaacg gcgcggattt cctgccttgg ttgtaaatct
     6961 gccaatcgga tctttgggtg tagccgaacc acagatccgc gcgggtttta aacaaatctt
     7021 cggcaatttt gcttttgaac gaaacctgca atttggtttc cgcacgtttc tgctgtccga
     7081 atttttcctg tacagtcgta ccgcgcgtcg gcgaacccgg ggcatagttg ggcgaattgt
     7141 tgtaccagag cggcataagg tacatcggat tgtgttcgcg tacgcccaac agcccgcgca
     7201 aatcgttttt gtccaagtcg tacatcaggc tcaaaggcgt atagatgtcg gcggtttcgc
     7261 ccgcactgtc ggcaggaagc gcatccccgc ctttttcaac aacaatgacc gcctcgccct
     7321 tatccaggct gctgcggacg gtttccgtca gattgagtac ggctttcgac tcctgccctt
     7381 cctgccctgc cgaagacgga agctgtgccg caaaaatcct gtcgtaacac gccaaacgcg
     7441 taacattgtc cgtcaaagcg gcgcattgca gcgcggtctc tccaaaagcg gatgccatcg
     7501 gcaacagtcc tgtcaaaaga atatagcgca tattccgtgt attcatctcc gcccccattg
     7561 tcggcatatt ggttttcaaa cggcatttta tagcggattc ggataaaaaa ccgcaccctt
     7621 tccgccattt cgggattttg cccccgcaat acagaaaacc ccgaaaccgt caggcttcag
     7681 ggttttccgc ttatcgcgta tcaaccgcct tggcggtttt gcaaaaatca agccagtgct
     7741 tttacttttg cagacagacg gcttttgtgg cgtgccgctt tgtttttgtg gaacacgccc
     7801 ttgtcggcga tgcggtcgat gactttgaca gactcttggt aaaccgcttg tgcggcagct
     7861 ttatcgcctg cttcgactgc tttcaatact tttttcactg cagtgcggaa tgcggtacgc
     7921 aggctagcgt tgtgggcgcg ttgtttgacg gactggcggg cacgtttgcg tgcttgtgcg
     7981 ctgtttgcca tattggaatc tcctggaaga aaaataaatt ctgtaaacgc gcaattttaa
     8041 agggggttta cattatatgc aagccttttc tctctatccg cccgtcggac accctgacag
     8101 cgcaaaaaaa cggcaaaatc gtgcggacgt tttccccctc ttttgaaaac aaggtaattt
     8161 tttaggaaaa tcaggtcaat atcgctttga gtttgcacaa atttacttta caatgcccaa
     8221 accttgcgcc ggggtgttta tggggcgtgg ggttttcttt ttttccaacc tctttcatca
     8281 ggaatctgcc cgtatgaaaa aatccgtatt agccgttttg gccgcgctgt ctctggcagc
     8341 ctgcggcggc agcgaaaaaa acgccgtgca acctcaagca gacgctgcgt ctgctgccaa
     8401 tgccgaagcc gctgccaccg atactctgaa catctacaac tggtcgaact atgttgacga
     8461 gagtacggtc gaagacttta aaaaagccaa caatctgaag ctgacttacg acttgtacga
     8521 aaacaatgaa acgctggaag ccaaaatgct gaccggcaaa tccggttacg atttggttgt
     8581 gccgggcatc gccttcctgc cgcgccaaat cgaggcgggc gcgtatcaaa aagtcaacaa
     8641 agacctgatt cccaactata aaaacatcga tcccgaactc ttgaaaatgc tggaaaccgc
     8701 cgacccgggc aaccagtatg ccgtccccta tttctccggc gtgaacacga ttgcgattac
     8761 ggcgaaaggc aaagagcttt tgggcggcaa gctgcccgaa aacggctggg atttgctgtt
     8821 caaacccgaa tacacccaca agctgaaatc ctgcggcatc gccctgtggg acaccccaag
     8881 cgaaatgttc ccgattttgc tgaactactt gggcaaagac cccaaaggct cgaatcctga
     8941 agatttgaag gcagcggcgg aagtgttgaa gtctatccgt ccggatgtca aacgtttcag
     9001 cccgtccatc attgacgaat tggcgcgcgg cgatatctgc ttggcggcag gtaacggcgg
     9061 cgatttgaac ttggcgaaag cacgttccga agaagtgaaa aacaacgtcg gcatcgaagt
     9121 gctgaatccg aaaggcatgg gcttctggat tgagtcttgg ctgattcccg ccgatgcgaa
     9181 aaacgtcgcc aatgcccaca aatacatcaa ctacacgctc gaccccgaaa tcgcggcgaa
     9241 aaacggcatc gccgtaacct tcgcccccgc cagcaaaccg gcgcgcgaaa aaatgcctgc
     9301 cgaactggta aacacccgtt ccatcttccc gaacgagcag gatatgaaag acggtttcgt
     9361 gatgccgcaa atgagcacgg atgcgaaaaa actgtcggtc agcctgtggc agaaaatcaa
     9421 agtcggcacc aactgatttg aagcattaaa aatgccgtcc gaacatcgtt cggacggcat
     9481 tttatattgg attgaaatag aaat
//
LOCUS       DAWHXZ010000063         9428 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000063 DAWHXZ010000000
VERSION     DAWHXZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9428
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.063"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(134..1576)
                     /gene="aldA"
                     /locus_tag="WIE35_000958"
     CDS             complement(134..1576)
                     /gene="aldA"
                     /locus_tag="WIE35_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ4153888.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1611..1811)
                     /locus_tag="WIE35_000959"
     CDS             complement(1611..1811)
                     /locus_tag="WIE35_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153889.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTAIYLGIN"
     gene            complement(1820..2209)
                     /locus_tag="WIE35_000960"
     CDS             complement(1820..2209)
                     /locus_tag="WIE35_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153890.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLVN
                     YSYLDNCLVTLTADRISVSFIREIQAA"
     gene            complement(2418..3335)
                     /locus_tag="WIE35_000961"
     CDS             complement(2418..3335)
                     /locus_tag="WIE35_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4153891.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYMEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLNFGINRSRFQIDADGGKVILISVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYACFTPILYREPVRIWNLSPNLRGLAASCLQAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLADALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3508..4308
                     /locus_tag="WIE35_000962"
     CDS             3508..4308
                     /locus_tag="WIE35_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ4153892.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLSGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4356..5132
                     /gene="mlaE"
                     /locus_tag="WIE35_000963"
     CDS             4356..5132
                     /gene="mlaE"
                     /locus_tag="WIE35_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ4153893.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5183..5677
                     /gene="mlaD"
                     /locus_tag="WIE35_000964"
     CDS             5183..5677
                     /gene="mlaD"
                     /locus_tag="WIE35_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ4153894.1"
                     /translation="MKKNILEFWVGLFVLLGVAAVGFLAFRVAGGAAFGNSGKTYTVY
                     ADFSDIGGLKANAPIKSAGVLVGRVGSIELDPKSYQAKVRLDLDSKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5714..6304
                     /locus_tag="WIE35_000965"
     CDS             5714..6304
                     /locus_tag="WIE35_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ4153895.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVSQIRQNATQVLSILKN
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDTQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGGK"
     gene            6355..6633
                     /locus_tag="WIE35_000966"
     CDS             6355..6633
                     /locus_tag="WIE35_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ4153896.1"
                     /translation="MHTELKNGILHIGGDITVKTLTAAAFTRFRQQCRLKDTCAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6630..7457
                     /locus_tag="WIE35_000967"
     CDS             6630..7457
                     /locus_tag="WIE35_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ4153897.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7582..7944
                     /locus_tag="WIE35_000968"
     CDS             7582..7944
                     /locus_tag="WIE35_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ4153898.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7941..8327
                     /locus_tag="WIE35_000969"
     CDS             7941..8327
                     /locus_tag="WIE35_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ4153899.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8324..8812
                     /locus_tag="WIE35_000970"
     CDS             8324..8812
                     /locus_tag="WIE35_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ4153900.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8895..9062)
                     /locus_tag="WIE35_000971"
     CDS             complement(8895..9062)
                     /locus_tag="WIE35_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ4153901.1"
                     /translation="MEDDVWPGHRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(9029..9409)
                     /locus_tag="WIE35_000972"
     CDS             complement(9029..9409)
                     /locus_tag="WIE35_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ4153902.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
BASE COUNT         2199 a         2625 c         2347 g         2257 t
ORIGIN      
        1 taaatttaat ccgctataca aagacaaaaa catcgacctc ggtcatgact gattgccgat
       61 gaagcaataa aaatgccgtc tgaacctgaa aacgggttca gacggcattt tctatcgggg
      121 ttttaaagcg gcattaaatg tcggtttcca aataaacgac ttgggtttgc agatattctt
      181 ccaaaccgtg tttgccgtcc gcgccgccga taccggattt tttccaaccg gcgtggaaac
      241 cctgcatcgc ttcaaagttt tcgcggttga tgtaggtttc gccgaattgc aggcggcggg
      301 taacgtagaa ggcttcgttt aaattagtcg tataaacaga actggtcaga ccaaactcgc
      361 aatcgttagc cagtgcgatg acctggtcga gcgtgtcgaa agcggaaacg ggcagcacgg
      421 ggccgaaggt ttcttctttc ataatgtcca tactgttgtc ggtgtcggtc agcagggtcg
      481 gctcgaagaa ataaccgcgt ccttcggcgc gtttgccgcc gcaaaccaat ttcgcacctt
      541 gtttgactgc ccgttccact ttttcggcaa cggctttgac ggcgcgctct tcaatcagcg
      601 ggcccatttc cagcgcgcct gcttcggctt cggcagggtt gccgtagcgt acgcctttca
      661 tggcggcggt cattttttca atgaatgcgt ctttcagact gctgtggaca taaacgcgct
      721 cggcgcagtt gcagatttga ccagtgttgc cgacgcgcga agccaagatg gatttcactg
      781 ccaagtccaa atccgcgtct ttcaaaacga tagcaggtgc tttgccgccg agttccagcg
      841 agactttggt gatgttggca gaagcggctt ccatcacttg gcggcccgct tcaacggagc
      901 cggtcaggct gaccatatcg acttgcggat gggcggacaa ggcattgccg atttccgcac
      961 ccggaccgtt cacaacgttg aacacgcctg ctggcagtcc gaccgcatcg acgatttcgg
     1021 cgaagatgtg gcagttgatc ggggttacgc tgctgggttt gacgacgatg gtgttgcccg
     1081 ttaccaaagc ggggcccatt ttgcgggcaa tcaggaagaa ggggaagttc cacggcaaaa
     1141 tgccggcgat tacgcccagc ggacgtttga acaataaaat attttcgcgc gggcggtcgc
     1201 tttggatgat ttcgccttcg taacgacgcg cccattcggc ttggtaatcg agatagtcgg
     1261 cggtaaacat gacttccacg cgcgccaagt ctttggtttt gccgccttcg gcaacgatgg
     1321 tgtcggtcag ctcgtcggcg cgtccgcgta tgccttgggc gattttgcgc aaatacgcgc
     1381 cgcgttcgac cgcaggcagg cgttcccaag ccggttgcgc cgcgcgtgcc gccgccacag
     1441 ctcggtcaac gtctgctttt ccgcctttgg gttcgcgggc gatggtttct tcggtggatg
     1501 ggttcaatac gtcgcgccat tcgccgttga aatcgttttc aaagcgtccg ttgatgtaca
     1561 tggccaattg tttcatttcg ggttctccag ttttgtagtc agatgtagtc ttagtttatt
     1621 cccaaataaa ttgccgtaca agtttctttt gaaatgttta gacgagaagc tactatgtct
     1681 gcatattgtt ctgctaattt atattcactc gaatgcaact gccgattatt ccaatctgca
     1741 ttcgcatcag taaaagcccc agcattgccg cctgtggcat aaccaatcgc taaactgcca
     1801 acggtatcca caacaggttt caggctgcct gaatttctct aataaatgaa acacttattc
     1861 tatcagccgt taatgtaacc aaacaattat caagataaga gtagtttact aaatactcat
     1921 cttcttcaaa ttgtatattt tgtagggtta gatgattatt ttttaattct gccgttattt
     1981 catacttgaa actatcatct atttttctaa atccaatatt tgaagttgat ttatccagta
     2041 tgtaatcatt aaagatttca taggataatt ccacttcata aaattgattt tcaaatattt
     2101 tgaatgggca gtatgatata aaacaatgta gttcataacc atttatttca atcaatactg
     2161 cctcttcaat ggtgttatct aactttttga ttttacattt atataacatt ttatcttgcc
     2221 tttagataaa ttgttttgtt ttttggattg ttattcaaag ttctccaaaa gtcttttcct
     2281 gattttgtat gtatcgtggt aacactacca ttttcattgg ttccaacaaa aataaattta
     2341 gattgacctt tggaatcagt acccaaaaac tgaacatatc cagacatgcg gatttcgggt
     2401 tttaaatcaa atgcggctta ccggttttct tttttggttt ccgcaggcgt gctgccgaaa
     2461 tattgcctga atgcctgagt aaagctggaa acgtggcggt agccgcacaa atacgcggtt
     2521 tcgcctatgc ttttgccccc gttttgcaac aggtagagcg cgtgctgcat ttgtttgtgg
     2581 tgcagccact cgcttgcggt aatgccgaaa tggtcgcgca tacggcgttg cagcgtcctt
     2641 tcgctgatgt tcagcgcgtc tgccagccgg ttgacttggt gtgcgccgtc ggcaaacgcg
     2701 gcatttaggg tgcggctgaa gtcttcagac ggcatagcgt ctgcttccgc cgtttgcccc
     2761 gccgccggcc cgatgccgtc tgaaaccgtg tcccacaaat ccgacagcag ccgcaaaacg
     2821 tccgcctcgc ggcgcaatgt ttcgcccaaa tgcccctttg ggacggcttg caggcaggat
     2881 gccgccaaac cgcgcaggtt tggggacaaa ttccatattc tgaccggttc gcggtaaagt
     2941 atgggtgtaa aacacgcata ctccggacgc agcagccatt gctccatacc tttaattgtc
     3001 attttgaccg ttttgccgcc tcgataaaga tagcggctaa acaggacttc ttccccgact
     3061 gaaatcagga tgaccttgcc gccgtccgca tcgatttgga atcggctccg gttgatgccg
     3121 aagttcaaag agccttccag caaaagcacg aacgacacat aaggttcggc gaggcggctg
     3181 ctgcaaaaat cgcaccgtgc cgttaccgtg ccgccgtgga tggaaatgcc gttggacaaa
     3241 gtatcgaaac ggtagctgcc ctccatatag ctgccgccgc cgcttttgac cagattgacg
     3301 aaccggctgc tgttcaaatg ttctgctttt tccatagttt tctgataaag gttatcattt
     3361 gaaagataac atttttcgcc acagcaaaca atccgccgcc gacaacggca ttttcacaac
     3421 acttaccgtc cgtatgccgt ctgaaaattg cccgtcttcc cgataagcct taaaatacac
     3481 gccgtcaaac ctaccgtccg tccgtacatg agcccatccc cctttatcga aatgaaagac
     3541 gtcgccttcg cgtatggcga ccgcccgatt ctgaacgaca tcaatttcag tattccgcaa
     3601 ggtaattttg ccgccgtgat gggcggttcg ggcagcggca aaaccacgct gatgcgactg
     3661 attacaggac agattcgtcc gcagtccggg caggttttga ttgaaggacg tgatttgtcg
     3721 ggcttttcgg ctgacgagtt gtatgaacac cgccgccgta tgggcgtatt gttccaacac
     3781 ggcgcgctgt ttaccgattt gtctgtattt gacaatattg cttttccgat gcgcgaactg
     3841 acgcaactgc cggaagcggt gattcgagat ttggttttgt tgaaactgaa cgcggtcggt
     3901 ctgcgcggtg tagaaaacct gatgccgtcc gagttgtccg gcgggatgtc gcgccgcgtc
     3961 gcgcttgccc gcacgattgc gctcgacccc gaaattatgt tgtacgacga accgtttacc
     4021 ggtctcgatc cgatttcctt gggcgtgatt gcccacttga tcagccgcgt caacaaggct
     4081 ttgcgttcga ccagtattat ggtcacgcac gacattgaaa aatctttgga aatcgtcgat
     4141 caggtgattt tcttggcgca cggcgaaatt atgttctccg gctcgccgca ggaaatgcgc
     4201 gaactggatt cgccttgggt gcgccagttt gtcggcggct tggcagacgg ccccgtggca
     4261 taccgttatc cggcgcaaac gtcgttgcag caggatttgc tcgggtaaat gagccggtgc
     4321 cgtctgaaac cgttgaactt tacatataga aatttatgaa ctttatccgt tccgtcgggg
     4381 cgaaaaccct cggccttatt caatccctcg gcagtattac gctgtttctg ctgaacatct
     4441 tggcgaaatc cggtacggct ttcgtccgtc cgcgcctgag cgtgcgccaa gtgtatttcg
     4501 ccggcgtgct gtcggtgctg attgttgccg tttcggggct gttcgtcggt atggttttgg
     4561 gtttgcaggg ttatacgcag ttgtcgaaat tcaaatccgc cgatattttg ggctatatgg
     4621 tcgcggcttc gctattgcgc gagctgggtc cggtattggc agcgattttg tttgccagca
     4681 gcgcgggcgg tgcgatgacc agcgaaatcg gtctgatgaa aacgaccgaa cagctcgaag
     4741 cgatgaatgt gatggcggta aaccctgtcg cccgcgtggt tgcgccgcgc ttttgggcgg
     4801 gcgtgttttc catgccgctt ttggcttcga ttttcaacgt ggcgggcatt ttcggtgcgt
     4861 atttggtcgg tgtaagctgg ctgggcttgg acagcggcat tttctggtcg caaatgcaga
     4921 acaacatcac gatacattac gatgtaatca acggtctgat taaatccgcc gcgttcggcg
     4981 tggcggtaac gctgattgcc gtgcatcagg gcttccattg cgtcccgact tcggaaggca
     5041 ttttgcgcgc cagcacgcgc acggtggttt cgtccgccct gacgattttg gcggtcgatt
     5101 ttatattgac cgcgtggatg tttacagatt gacaggccgt ctgaagacga aacgcggaac
     5161 atcggatatt caaggaactt taatgaaaaa gaacatattg gaattttggg tcggactgtt
     5221 tgtcctgctc ggcgtggcag cggtcggctt tctcgctttc cgcgtggcag gcggcgcggc
     5281 cttcggcaat tccggcaaga cttataccgt atatgccgat ttcagcgaca tcggcggact
     5341 gaaggccaac gcgccgatca aatccgcagg cgtattggtc ggacgcgttg gttccatcga
     5401 gcttgacccg aaatcctatc aggcgaaagt ccgtctcgat ttggacagca aatatcagtt
     5461 cagtagcgac gtttccgcac aaatcctgac ttcgggactt ttgggcgagc agtacatcgg
     5521 gctgcagcag ggcggcgaca cggaaaacct tgctgccggc gacaccatct ccgtaaccag
     5581 ttctgcaatg gttctggaaa accttatcgg caaattcatg acgagttttg ccgagaaaaa
     5641 tgccgacggc ggcaatgcgg aaaaagccgc cgaataaacg tatgccatca tattcaaact
     5701 aggacactta atcatgaaaa aatcctccct catcagcgca ttgggcatcg gtattttgag
     5761 catcggcatg gcatttgccg cccctgccga cgcggtaagc caaatccgtc aaaacgccac
     5821 tcaagtattg agcatcttaa aaaacggcga tgccaacacc gcccgccaaa aagccgaagc
     5881 ctatgcgatt ccctatttcg atttccaacg tatgaccgca ttggcggtcg gcaacccttg
     5941 gcgcaccgcg tccgacacgc aaaaacaagc gttggccaaa gaatttcaaa ccctgctgat
     6001 ccgcacctat tccggcacga tgctgaaatt aaaaaacgcc aacgtcaacg tcaaagacaa
     6061 tcccatcgtc aataaaggcg gcaaagaaat catcgtccgc gccgaagtcg gcgtacccgg
     6121 gcaaaaaccc gtcaacatgg acttcaccac ctaccaaagc ggcggtaaat accgtaccta
     6181 caacgtcgcc atcgaaggcg cgagcctggt taccgtgtac cgcaaccaat tcggcgaaat
     6241 tatcaaagcg aaaggcgtgg acggactgat tgccgagttg aaagccaaaa acggcggcaa
     6301 ataaccgaca aaccataccg tccgagggcg gtatgggttt tccgagcaaa cgctatgcat
     6361 acagaactca aaaacggaat attgcacatc ggcggcgaca tcaccgtcaa aaccctgacc
     6421 gccgccgctt ttacccgctt cagacagcaa tgtcgtctga aagacacctg cgcggttgat
     6481 ttgagcggcg tcggacgcgc cgattccgcc tgcgtgtcgc tgctgctcga agtgctgcgc
     6541 ggttgcaaag gcagcgtcag gctgaccggc attcccgaat ccgtgcgcgc gctgtccgaa
     6601 ctgtacgaaa tcaaagactg gctgaaatca tgaaaaaaac cgcctatgcc ttcctcctgc
     6661 tgatcgggtt cgcttccgcc cctgcatttg ccgaaacccg ccccgccgac ccttatgaag
     6721 gctacaaccg cgccgttttc aaattcaacg accaagccga ccgctacatt ttcgcccctg
     6781 ccgcgcgcgg ctaccgcaaa gttgcgccga aacccgtccg cgccggcgtg tccaattttt
     6841 ttaacaacct gtgcgacgtg gtcagcttcg gtagcaatat cttgcgcttg gacatcaaac
     6901 gcgcaagcga agaccttgtc cgcgtcggca tcaacaccac tttcggtttg ggcgggctta
     6961 tcgacatcgc cggcgcgggc ggcattcccg acaataaaaa caccttgggc gacacgtttg
     7021 cttcgtgggg atggaaaaac agcaattatt tcgtgttgcc cgtcttaggg ccgtccaccg
     7081 tccgcgacgc gctcggcacg ggtattacct ccgtttattc gcccaagaat atcgtcttcc
     7141 gcacccctgt cggacgctgg ggcacgactg ccgtatccgc cgtcagtacg cgcgaaggtc
     7201 tgctcgattt gaccgacagt ctggacgaag ccgccatcga caaatacagc tacacgcgcg
     7261 acctctatat gaaagtccgt gcgcggcaga ccggtgcaac acctgccgaa ggtacggaag
     7321 ataacatcga catcgacgaa ttggtcgaaa gtgccgaaac cggcgcggcg gaaactgccg
     7381 ttcaagaaga ttccgtatcc gaaacacagg cagaagcagc aggggaagcc gaaacgcaac
     7441 ctggaacaca accttaaagc catgccgtct gaaaatccgt gttcggacgg cattcccttc
     7501 tgtcggaacg gacagtccga aaccacagat tgggctataa tctctttttt gcgcataccg
     7561 cgcgacagca aaggaaaaac catgtacgaa gtcaaccgca gcgtattcgt cctcatccct
     7621 ttagaacctt tttggaactg gctgcaaacc ctgccgggca accacctcga cggactgact
     7681 cttgaagaca tccaagccga cgccaattcc taccttgtcc gcccgtgcga aaccgccgac
     7741 gaagtatggg acgaaatcga agcccgtttt gaagacattt tcgccgccga acttgccgac
     7801 tggtgcgaag acgaacgcga gtggcctgcg ctcgatgccg acattttcaa cgaatggttc
     7861 gacatccagc tttctaccgt catcaccgac ctcgaacacg aaccgcttgc ccgcgaagcc
     7921 ttccaaccca tcaacctgaa ctgatgaagc tgaccgtccg caactaccat ctcgacggct
     7981 acggccatgt caacaatgcg cgctacctcg aatttttcga agaagcgcgc tgggcgtttt
     8041 ttgaagaacg cggactgctg cacgagttgg caggcctcat actgattgtc gcccgcatcg
     8101 acatccgata cagccgcccc gccgtcgaag gcgacgtatt gcagttttca tgccgcctga
     8161 aaacccccgg cacgcgccgt attgtgctga cccagacgat tacactgcca aacggcaaaa
     8221 ccgccgcaga agccgacatc accctgatgc ccgtccacgc cgccacacaa cgcaccgtca
     8281 gcctgcccgc cacccttgcc cgcgcattgg aagccttgtc cgaatgaaaa aaatactcac
     8341 cgccgccgtc gtcgcactga tcggcatcct ccttgccatc gtcctcatcc ccgacagcaa
     8401 aaccgcgccc gccttctccc tgcccgacct gcacggaaaa accgtttcca acgccgacct
     8461 gcaaggcaaa gtaaccctga ttaatttttg gtttccctcc tgtccgggtt gtgtgagcga
     8521 aatgcccaaa atcattaaaa cggcaaatga ctataaaaac aaaaacttcc aagtccttgc
     8581 cgttgcacaa cctatcgatc cgatagaaag cgtccgccaa tatgtcaaag actacggttt
     8641 gccgtttacc gtcatgtatg atgcggacaa agctgtcgga caggcgttcg gcacacaggt
     8701 ttatccgact tctgtcctca tcggcaaaaa aggcgaaatc ctcaaaactt atgtcggcga
     8761 acccgatttc ggcaaactct accaagaaat cgataccgcg ctggcacaat agcgatgccg
     8821 tctgaagcgc aaaaaaaaca gaaacgatgt tcctgttttt tcagatcatc cgccccgaca
     8881 cgttcagacg gcattcaacc ttccggcaga ttctttttca ccactgccgg attgcctgcc
     8941 gccaaagagt agggcggaat gtcttttgta accaccgcgc ctgcgcccac gaccgaaccg
     9001 cgtccgacgg ttacgcccgc cattacaatc accctgtgcc ccggccagac atcgtcctcc
     9061 aacgtaatcg gacggatttc cgtgtagccc tcaaagcgtt ttttgaacgg tcaaacttgt
     9121 ggttatttga ataaaacaga cattccggct ccatcatcac atttttgccg accaccagcc
     9181 cacggcagat ttcacagttt gccccgatgc ccgagccgtc gcccaaaacc gtatccggaa
     9241 acacatacgc cccgcgttcg atattgaccc cgcgtccgat atgcggagaa acccgccgcg
     9301 ccaaaaaacc gcgcacccgt ctgccgacga tgccgatgcc gcgcatatgt gacggcggca
     9361 accccgaacc ggcaaaacgc agcagcagga aaaagaattt tgatcccatc aaacctccct
     9421 caaaagaa
//
LOCUS       DAWHXZ010000064         9417 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000064 DAWHXZ010000000
VERSION     DAWHXZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.064"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            215..1648
                     /gene="lpdA"
                     /locus_tag="WIE35_000973"
     CDS             215..1648
                     /gene="lpdA"
                     /locus_tag="WIE35_000973"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ4153903.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELLTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            1743..2030
                     /locus_tag="WIE35_000974"
     CDS             1743..2030
                     /locus_tag="WIE35_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153904.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            2129..3295
                     /gene="sucC"
                     /locus_tag="WIE35_000975"
     CDS             2129..3295
                     /gene="sucC"
                     /locus_tag="WIE35_000975"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ4153905.1"
                     /translation="MNLHEYQAKELLAGYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            3306..4196
                     /gene="sucD"
                     /locus_tag="WIE35_000976"
     CDS             3306..4196
                     /gene="sucD"
                     /locus_tag="WIE35_000976"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ4153906.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(4292..4759)
                     /locus_tag="WIE35_000977"
     CDS             complement(4292..4759)
                     /locus_tag="WIE35_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ4153907.1"
                     /translation="MDVQKQYPFETVAVCVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAYSPYTKNLSAGKQRRNERGIWQRRFYEHTVRDETDLQCYADYIHSNPIKQRMSRA
                     KTESCKNNISLFKCDTYHSLTSKKYVQPDLRVCSCLTDKYSDCLKGQKIMWVC"
     gene            complement(4731..4874)
                     /locus_tag="WIE35_000978"
     CDS             complement(4731..4874)
                     /locus_tag="WIE35_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153908.1"
                     /translation="MARYRRNFIAGGTFFFTVKLADPKSRLLVEHIGFAYGLYGCAKT
                     ISL"
     gene            complement(5160..6362)
                     /gene="dcm"
                     /locus_tag="WIE35_000979"
     CDS             complement(5160..6362)
                     /gene="dcm"
                     /locus_tag="WIE35_000979"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ4153909.1"
                     /translation="MLIPQTSLFDQENNRIISQRKLLEQSLLQGIPLNELIRQNQMSY
                     DDLIYIHHDKKPFEKRKNLVLSDFQKKENHIPIISFFSGAGGLDLGFEAAGFQHSMLI
                     EKIPLFCDTLRLNRPNWSVFNADVSETEEMVELIRNNIGSLSFDGVFIGGPPCQPFSI
                     ASNQRFSKTDKNFKRVGFEHKQNGNLLFNYVELIKIFRPKVFLIENVSGLMDIDNGKQ
                     LASVYCDLQQQGYSLYKPQIINSEYFHVPQQRIRLFIIGYLGDKKFSPLQPTNTPLAC
                     GNTFEIPIINQHNHITREHSAESILRYMELAYGQRDKKGRVDRLDPSKPSKTIIAGGT
                     KGGGRSHLHPYIPRTLSVRESARLQTFPDDYIFTGAVARQFTQVGNAVPPILAAQLAT
                     AIKHSYFE"
     gene            complement(6375..7559)
                     /locus_tag="WIE35_000980"
     CDS             complement(6375..7559)
                     /locus_tag="WIE35_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ4153910.1"
                     /translation="MIEWRFDQGRLDYFRFDEIKKIAKGLYGINQCPKPRAKDIDIVR
                     LSLTQETNLPFAPNSSQYPVWRNYGRVFKCLLLATEHNGLICTTDLCADLVQNDEISC
                     DDYLLHFATHFSYPSPAFSGYQPSENRVFPVLAIIRALISKYKETGVNFLDMDEIIYK
                     IASNNSITGIASFSFSQLIMNKEAIKYDPRQIRELVKFISQFSFLSWYENKLFLDITD
                     FSELHLLENQFIADTQPQERLDSLEIMRLGKTNNNFLLMNEIVSLSIDDQAFSEGGKI
                     KVTHLITERNSILRKIYFSKTQNAQICRMCGLDTQRSYEWSEHIIEVHHLLPLSSPVR
                     VEDKTTSLKDLVGICPTCHRATHKYYTFWLNLHKKKDFVSYEEAYHVYEEAKSKVKRI
                     YK"
     gene            complement(7601..>9417)
                     /gene="uvrA"
                     /locus_tag="WIE35_000981"
     CDS             complement(7601..>9417)
                     /gene="uvrA"
                     /locus_tag="WIE35_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ4153911.1"
                     /translation="ETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPN
                     LERRYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTK
                     THQFFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIR
                     LASQIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREA
                     DFVVDMGPGAGEHGGNVLIADTPENVAKCENSITGQYLSGKKSIAVPSERTPVNPDRM
                     LVLKGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPA
                     PYDDIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVG
                     RFSFNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQ
                     VLDMTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELS
                     KRDTGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVD
                     LGPEGGDSGGRIIAQGSPEEVAKVKGSYTGKYLKVVLG"
BASE COUNT         2507 a         2089 c         2308 g         2513 t
ORIGIN      
        1 actatattta gatgtagcgt aatgtagtat cgtgctacaa taggctcaac aaacgattga
       61 gaccgtctga aacatttgat tcgaatgaat cggcagatat ggactttcag acggcttttt
      121 cttaaaacca tcaaaacgca gtcattcaaa ataaaaaaga aacgaaaagt atcgttttta
      181 ttttgagata ctgctaaaag caaaggatga cacgatgtct caatatgatg tagtagtgat
      241 tggtgcaggc ccgggcggat acgttgccgc catccgtgcc gcacaactgg gtttcaaaac
      301 tgcctgtgtc gatgcaggcg ttaacaaagc aggcaatgcc cctgcattgg gcggtacttg
      361 cttgaacgta ggttgtatcc cttctaaagc cctgttgcaa tccagcgaac atttccacgc
      421 agcgcaacac gagtttgccg aacacggtat cactgtcggc gacgtaaaat tcgacgtggc
      481 caaaatgatt gagcgcaaag atgccatcgt aaccaaactg accggcggcg tgaaattcct
      541 gttccagaaa aacaaagtaa ccagcctgtt cggtacggct tcctttgccg gcaaaaatgg
      601 cgatgcttac caaatcgaag tcgataacaa aggtgagaaa accgttatcg aagccaaaca
      661 cgtcatcgta gcgaccggtt ccgtaccgcg tccgctgcca caagtcgcta tcgacaatgt
      721 gaacgtattg gacaacgaag gcgcattgaa cctgaccgaa gtacctgcca aactcggcgt
      781 gatcggttcc ggtgtgattg gtttggaaat gggttccgta tggaaccgcg tgggtgcgga
      841 agttaccatt cttgaagccg cgccgacctt cctggctgcc gccgaccaac aaatcgccaa
      901 agaagccttc aaatacttca ccaaagagca aggtctgagc atcgaattgg gcgtgaaaat
      961 cggcgacatc aagtctgaag gcaaaggtgt ttccgttgct tacgaaactg ctgccggcga
     1021 agccaaaacc gaagtattcg acaaactgat cgttgccatc ggccgtattc caaacaccaa
     1081 aggcctgaac gccgaagccg taggcttgga aaaagacgag cgcggcttta tcaaagtaga
     1141 tggcgaatgc cgtaccaacc tgcctaacgt atgggcaatc ggcgacgtgg ttcgcggccc
     1201 gatgttggca cacaaagcca gcgacgaagg cgttgccgtt gccgaacgca ttgccggtca
     1261 aaaaccgcat atcgacttca acaacgtacc gttcgtgatt tacaccgatc ctgaaatcgc
     1321 ttgggtgggt aaaaccgaag agcagctcaa agccgaaggc gtggagtaca aaaaaggtac
     1381 ttcaggtttt ggtgcgaatg gtcgcgcatt ggcaatgggc aaagccaaag gtacggttaa
     1441 agtgttggca gacgccaaaa ccgaccgcat cttgggcgta cacatgattg gtccggttgt
     1501 cagcgaattg cttaccgaag gcgtgactgc gctcgaattc ttcgccagca gcgaagacat
     1561 cgcccgcatt atccatgccc acccaacctt gtccgaagtg gttcacgaag ctgcattggc
     1621 ggccgacaaa cgcgctttgc acggttgata gacattaagg cctccgacaa attgaatgtt
     1681 ccgagagctt cgttttctga tttataattc cgtcagacaa acaaacagca tttacattcg
     1741 ttatgaacaa agaaatagtc ggtattttct ttataccgat gggcatcatc agcatgtgta
     1801 tggccgcatt gtggcagatg tatgtgatga tgaccgaaac ttatacgctc aaccgtttca
     1861 aagataaaga attggtttgg cgcgtggcat tgttgtttat cagtttcagc cttgccgttt
     1921 atctgctctg tccgaattcg cgtaaaaaag gcattgtctt ttttattctc gggggaggcg
     1981 gtgcagccat gtatctgctg gcgcggatgt ggttgccttt cagcaagtga aacgacgatt
     2041 ttccgaccgc cgaaaggtag tctgaaacgc acgggcttgc catttggagg caaactcggg
     2101 gcattacact aatctaaagg agaaatccat gaatttacac gagtatcagg ctaaagaact
     2161 gctggctggc tacggtttgc ccgtacaagg cggtattttg gcacacaacg gcgaagaagc
     2221 cgctgcagct tacgacaaat tgggcggcaa attcgctgtt gtcaaagcac aagtacacgc
     2281 cggcggccgc ggtaaagcgg gcggcgtaaa agtcgttaaa agccgcgaag aagctaaaga
     2341 agtggctgaa agcctgattg gcaccaactt ggtaacttac caaaccgatg ccaacggtca
     2401 acctgtcaac agcgttttgg tttgcgaaga tatgtatccg gttcaaaccg agctgtactt
     2461 gggcgcagtg gttgaccgtt ctacccgccg cgttacattc atggcttcta ccgaaggcgg
     2521 tgtggaaatc gaaaaagttg ctgccgaaac tccagaaaaa atcttcaaag taaccgttga
     2581 tccgctggtc ggcctgcaac cttgccaagc tcgcgaagtt gccttccaac tgggcttgaa
     2641 agacaaacaa atcaacgagt tcgtcaaact gatgaccggc gcgtacaaag cgtttgtcga
     2701 aaacgacttc gccctgtttg aagtcaaccc gctggcagtt cgcgaaaacg gcgcactggc
     2761 ttgcgtggac ggcaaaatcg gcatcgacag caacgcgctc taccgcctgc cgaaaatcgc
     2821 cgaattgcgc gacaaatctc aagaaaacga acgcgagctg aaagcttccg aattcgacct
     2881 gaactatgtt gccctggaag gtaatatcgg ctgtatggtt aacggtgccg gtttggcgat
     2941 ggccaccatg gacatcatca aactgaaagg cggccaacct gccaacttct tggacgttgg
     3001 cggcggcgca accaaagacc gcgtggttga agcgttcaaa ctgattctgg aagacaaatc
     3061 cgttcaaggc gtattgatca acatcttcgg cggtatcgta cgttgcgaca tgattgcgga
     3121 agctatcgtg gcagccgtta aagaaatcaa cgtcgacgtt cctgtcgttg ttcgtttgga
     3181 aggcaacaac gccgaactcg gcgcgaaaat cctgaacgaa tcaggcctga aactgacttc
     3241 tgcagacggc ctgaatgacg cagccgaaaa aattgttgca gccgtaaacg cctaaggaga
     3301 aaagaatgag cgtattgatt aataaagaca ccaaagtatt ggttcaaggt ttcaccggta
     3361 aaaacggtac tttccactcc gaacaagctc tggcttacgg cactaaagtt gtcggcggcg
     3421 ttaccccggg caaaggcggt caaacccacc tgaacctgcc tgtattcaac accatgaaag
     3481 aagctgttaa agaaaccggc gcggatgcat ccgtaattta cgttcccgct ccgtttgtgt
     3541 tggattctat cgttgaagcc gttgattcag gcgtaggctt ggtcgttgtg attaccgaag
     3601 gcgtacctac tttggacatg ctcaaagcca aacgttattt ggaaaccaac ggcaacggta
     3661 cccgtttggt cggccctaac tgcccgggcg tgattactcc gggcgagtgc aaaatcggta
     3721 ttatgccggg ccacatccac actccgggcc gcatcggtat tatttcccgt tccggtacat
     3781 tgacttacga agccgtggca caaaccacca aactgggctt gggtcaatca acctgtattg
     3841 gtatcggcgg tgacccgatt cctggtatga accaaatcga cgcactgaaa cttttccaag
     3901 aagacccaga taccgacgcc atcatcatga tcggtgaaat cggcggtact gcggaagaag
     3961 aagcagccga atacatccaa tccaacgtaa ccaaacctgt tgtaggttac attgctggtg
     4021 ttaccgctcc taaaggcaaa cgtatgggcc acgccggtgc gattatttcc ggcggcaaag
     4081 gtactgccga ggaaaaattc gccgctttcg aaaaagccgg tatcgcttac acccgcagcc
     4141 ctgccgagtt gggcacgacg atgctggaag tgttgaaggc aaaaggtttg gcataatcag
     4201 gtttgacaac tgattaaaca tcaaatgccg tctgaaaccg gaaatcgggt ttcagacggc
     4261 attttgtttg tcatttcaaa aagaggcagc ctcaacatac ccacattatt ttttgccctt
     4321 tgaggcagtc agaatattta tcggtcaaac aggagcaaac ccgtaagtca ggttgcacat
     4381 atttttttga agtcagggaa tgataagtat cgcatttgaa aagactgata ttatttttgc
     4441 aggattctgt tttagcacgc gacattcttt gtttgatagg attagaatgg atatagtccg
     4501 cgtaacactg caaatccgtt tcatcgcgca cggtgtgttc gtaaaaacgc cgttgccaaa
     4561 taccgcgttc gttccgccgc tgtttgccgg cggataagtt tttagtataa ggggaatatg
     4621 cggagaattt ggttttaatc agccgccgga gtagggaata atccgcatcg tcgggcggca
     4681 gcgtccaaat ggcgtggata tggttcggca gcacgcatac ggcgacggtt tcaaagggat
     4741 attgtttttg cacatccata taagccgtac gcgaagccga tatgttcgac aagcaggcgc
     4801 gatttcgggt cggcgagttt gacggtgaaa aagaatgtgc cgccggcaat gaagtttctg
     4861 cgataacgcg ccataggatt tttgtttgtg gtttgggtgt tggagaagga atggtttgtt
     4921 gtttaaggtt ttaactttgt tggagctgat gttttcagac gacattggag gccgtctgaa
     4981 agcgctggct tcaacgaagt taaaagttgg aacggcaaac cgttaatcaa ccaaccgcgt
     5041 acgtaccgca cacacactgc acgttggctt tgaagtttcg tagactcaca ggtaggtgtg
     5101 gcgcaaccac gcatacggtg ggttgggtat aaaggctaca acttgctata gcttgcttgc
     5161 tattcaaaat aagaatgctt aattgctgtt gctaattgag cagcaaggat gggaggaacg
     5221 gcattaccaa cctgcgtgaa ttgccgcgca accgctcctg taaaaatata atcatcagga
     5281 aacgtttgta atcgtgcact ttcacgaaca gataacgttc ttgggatata tggatgtaag
     5341 tgagaccttc ctccaccttt agtgccacca gcaataatag ttttagatgg tttgcttgga
     5401 tctaacctat ctacacgtcc ctttttatca cgttgcccat aagctaattc catatagcgt
     5461 aaaatcgatt cagccgaatg ttctcttgta atatggttat gttggttaat aattggaatt
     5521 tcaaatgtat taccacatgc caatggggta ttggttggtt gtagcggact aaatttttta
     5581 tctcctaaat aaccaattat aaatagccta attctctgtt gaggtacatg aaaatattca
     5641 gaattaatta tttgaggttt atagagtgag tagccttgct gctgtaaatc acaatataca
     5701 ctagctaact gtttaccatt atctatatcc atcaatccag agacattttc aataagaaat
     5761 actttaggac gaaaaatctt aatcaattca acgtaattaa ataatagatt accattttgc
     5821 ttatgctcaa agccgacacg tttaaaattt ttatcagttt ttgagaatct ctggtttgag
     5881 gctatggaga aaggctgaca gggaggacct ccaataaata caccatcaaa acttaaagac
     5941 ccaatattat ttctgattag ttcaaccatt tcttctgttt cagaaacatc agcattaaac
     6001 acagaccaat tagggcgatt aagacgcaat gtatcacaaa ataaaggtat tttttcaatc
     6061 agcatagaat gttgaaagcc tgctgcttca aaccctaagt ctaatccacc ggctcctgaa
     6121 aaaaaactaa ttatcggaat gtgattttcc tttttttgaa aatcacttag aaccaaattc
     6181 tttctctttt caaaaggctt tttatcatga tgaatataaa ttaaatcatc atagctcatt
     6241 tgattttggc gtattagttc attcaatgga ataccttgta ataaactttg ttctaataat
     6301 tttctttgtg aaataattct attattttct tggtcaaata aagaggtttg cggtattaac
     6361 atagatactc ctatctattt ataaattctt ttaactttac ttttagcttc ttcatataca
     6421 tgatatgctt cttcataact aacaaaatct tttttcttgt gcaaattaag ccaaaaagta
     6481 tagtacttgt gtgtggcacg atgacaggtt ggacagatac caaccaaatc ttttaatgaa
     6541 gtagttttat cttctaccct tacaggagaa gatagtggta atagatggtg tacttcaatg
     6601 atatgttcgc tccattcata gctcctttgc gtatctaatc cacacatgcg acatatttgt
     6661 gcattttggg tcttgctaaa atagattttt cttaaaatac tattacgttc tgtaattaaa
     6721 tgggtgactt taattttacc accctcacta aacgcctgat catcaataga taatgaaacg
     6781 atttcattca tcaataagaa attattattt gtcttaccca atctcattat ctctaaacta
     6841 tctaatcttt cttgtggttg tgtatctgca ataaattggt tttctaataa atgtagttca
     6901 ctaaaatctg taatatctaa aaacaactta ttttcatacc aagacaaaaa agagaattga
     6961 ctaataaact ttaccaactc tctaatttga cgaggatcat atttaattgc ttctttattc
     7021 ataattaact gtgagaatga aaaagaagca atacctgtaa tactattatt actagcgatt
     7081 ttgtaaataa tttcatccat gtccaaaaaa ttaactcctg tttccttata ttttgaaatt
     7141 aaagctctaa taattgccaa aacagggaaa acccgatttt cagatggttg atatcctgaa
     7201 aaagctggtg atgggtagga aaaatgtgtt gcaaaatgta gtaaataatc atcgcaacta
     7261 atttcatcat tctgaactaa atcggcacat aaatctgtag tacagattaa cccattatgc
     7321 tccgttgcga gtaacaaaca tttaaaaaca cgaccataat tacgccaaac tggatattgt
     7381 gaactgttag gggcaaaagg taagtttgtc tcttgagtca ggcttaatct tacaatatca
     7441 atatcttttg cacgtggctt tggacattga ttaattccat ataatccttt tgcaattttt
     7501 ttaatttcat caaatctaaa ataatctaat ctaccttgat caaaacgcca ttcaatcatt
     7561 tttaacctct atgaaatatc atttttcagt aatctgaaaa ttatcctaaa accaccttca
     7621 aatacttccc agtataactc cccttaacct tcgccacttc ctcagggcta ccttgggcaa
     7681 taatcctccc cccgctatcg ccaccttccg gtcctaaatc tacaatccaa tccgcagttt
     7741 taatcacatc cagattatgc tcaataatca caatcgagtt gcctttgcct ttcagacggc
     7801 ctatgacttc cagcagcagg gcgatgtcgg cgaagtgcag gccggtggtg ggttcgtcga
     7861 ggatgtagag cgttctgccg gtgtcgcgtt tggagagttc caaggcgagt ttgacgcgct
     7921 gggcttcgcc gccggagagg gtggtggcgg actggccgag gcggatgtag ccgagaccta
     7981 cgtccatcag ggtttgcagt ttgcgcgata cggtggggac ggcgtcgaaa aattcgcggg
     8041 cttcttcgac ggtcatgtcg aggacttggc tgatgttttt gcctttgtat tggatttcga
     8101 gggtttcgcg gttgtagcgt ttgccgtggc agacttcgca ggggacgtac acgtcgggca
     8161 ggaagtgcat ttcgacttta atcacgccgt cgccttggca ggcttcgcag cggccgcctt
     8221 tgacattgaa ggagaatctg ccgacgttgt agccgcgttc gcgcgagagg gggacgccgg
     8281 cgaagagttc gcggatgggg gtgaacaggc cggtgtaggt ggcggggttg gagcgcgggg
     8341 tgcggccgat gggggattgg tcgacgttga tgactttgtc gaggtgttcg aggccgcgga
     8401 tgtcgtcgta tggggcgggt tcttcttggg cgcggttgag ttcgcgggcg gtgattttgg
     8461 cgagggtgtc gttaatcagg gtggatttgc cgctgccgga tacgccggtg atgcaggtaa
     8521 tcaaaccgag cggcagttcg agggtaacgt ttttgaggtt gttgccgcgc gcgcctttga
     8581 ggacgagcat ccggtcggga ttgacgggtg tgcgttcaga cggcacggca atggattttt
     8641 tgccgctgag gtattgtccg gtaatggagt tttcgcattt ggcgacgttt tcgggtgtgt
     8701 cggcaatcag tacgttgccg ccgtgttcgc cggcgcccgg gcccatatcg acgacgaaat
     8761 cggcttcgcg gatggcatct tcgtcgtgtt cgaccacaat cacgctgttg cctaaatcgc
     8821 gcaggcgttt gagggtggcg agcaggcggt cgttgtcgcg ctggtgcagg ccgatggagg
     8881 gttcgtccaa aacgtacatt acgccggtca ggccgctgcc gatttggctg gcgaggcgga
     8941 tgcgctgggc ttcgccgccg gaaagggttt cggcggaacg ggagagattc aggtaatcca
     9001 gcccgacatt aatcaaaaag ccgagacgtt cggtgatttc tttgaggatt ttttcggcga
     9061 tttgtttttt gttgccgtct aaatccagtg tttcaaagaa ttggtgggtt ttggtgagcg
     9121 gccaggcgga aacttcgtgc aacggctcac cgctgacgta aacgtagcgg gcttctttgc
     9181 gcaaacgtgc gccgccgcag ctcgggcagg cgcggtggtt ttggtattcg cgcagttttt
     9241 cgcgcacggt ttcgctgtcg gtttcgcggt agcggcgttc gagattgggg atgatgcctt
     9301 cgaaggcgtg gctgcggttg aaggtggtgc cgcgttcgga caggtaagtg aaatcaatga
     9361 cttccttgcc cgagccgtgc aacacgactt ttttgatttt tgcaggtagg gtttccc
//
LOCUS       DAWHXZ010000065         9103 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000065 DAWHXZ010000000
VERSION     DAWHXZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.065"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(60..1316)
                     /locus_tag="WIE35_000982"
     CDS             complement(60..1316)
                     /locus_tag="WIE35_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ4153912.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLFDEEETAFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQPAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(1664..2005)
                     /locus_tag="WIE35_000983"
     CDS             complement(1664..2005)
                     /locus_tag="WIE35_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153913.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2208..3065)
                     /locus_tag="WIE35_000984"
     CDS             complement(2208..3065)
                     /locus_tag="WIE35_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ4153914.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFTNAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPQGFHAFEPPLNPYGWRDVYIQD
                     WAEYGFERQEVRYHLLDRMAYLAPARAAETETLNETEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3126..3941)
                     /locus_tag="WIE35_000985"
     CDS             complement(3126..3941)
                     /locus_tag="WIE35_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ4153915.1"
                     /translation="MTTLKPALPDYLGNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4005..4175
                     /locus_tag="WIE35_000986"
     CDS             4005..4175
                     /locus_tag="WIE35_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153916.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCTSEPAVCHP"
     gene            4185..4970
                     /locus_tag="WIE35_000987"
     CDS             4185..4970
                     /locus_tag="WIE35_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ4153917.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDGWLESGDVVAANPKVLAQMLKIISAHV"
     gene            5240..7335
                     /locus_tag="WIE35_000988"
                     /pseudo
     CDS             5240..7335
                     /locus_tag="WIE35_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            7459..9009
                     /locus_tag="WIE35_000989"
     CDS             7459..9009
                     /locus_tag="WIE35_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ4153918.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGSFINSEGQFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLITAVKGEASGLFTHNPVLNIKTFRFTLPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEARASIAD
                     INETLRLMVDSTVQSMAREKYLTLDGNQIDTVISLKNNALKLNGKTLQNEPDPDFDEG
                     GMVSEPQQ"
BASE COUNT         2282 a         2159 c         2410 g         2252 t
ORIGIN      
        1 gatgaaatgc cgtctgaacg ctttgaaaag ggttcagacg gcattttgtt taaacagggt
       61 tatttgcgtt gcaatacggc ggcgaagaag ccgtcggttt ggtgtcgggc ggagttgagg
      121 cgcaggtatt tgccggtatc caaatcgatt ttcaaaccgg caagcagttc ggcgcagttg
      181 acgggttcaa attcgggatg ttcggacagg aaacgttcga cttgcagctc gttttcttcg
      241 ggcaggatgc tgcaagtggc gtaaaccaag cggccttgcg gttttaccag tttggaggcg
      301 gcatcgagga tgctgtgttg ctgttccaaa aggttggcca cggtttcggc ggattggcgg
      361 tatttgaggt cgggattgcg gcgtaaagta cccaaaccgg agcagggcgc gtccaccaac
      421 acacggtcgg ctttgcctgc cagtcgggcg atacggctgt cgtgttcgct gctgatgcgt
      481 tcggggtgga tattggtcag tccggcgcgg gtcatacgcg gtttgaggtt ggcaaggcgt
      541 ttttcggcga tgtcgaaggc gtagattctg cctttattgg ccatttgcgc accgacagcc
      601 aaggttttgc cgccggcacc ggcacagaaa tcgacaatga tttcgccgcg ttttgcgccc
      661 accaataaag caagcagctg gctgccttcg tcttggactt ccagtgtgcc gtctaaaaac
      721 agttcgtgtt tgttaagcgc gattttgttt ttcaggcgga tgccccaagg cgaataaggc
      781 gttgcctctg catcggcact ttcggcttgc aacagcggca gcactttatc gcgtttgcct
      841 ttcaaagtgt tgacgcggat gtcgagcggg gcaggctggt tgatgctgcg gccgaaggcg
      901 aggatttctt cttcacgcca atgctgttgc agttgttcga ccagccattg cggcaattct
      961 gcggcggtat tcaggccgtc tgaaaactcg gttttacggg ctttcaaatt gccgaggaac
     1021 gctgtttctt cttcatcaaa caggtctttg atttggctga tgttggtgct tctgccgaga
     1081 accagtgcgg cgagtgcggc tttgcgcggc tgtgcgtgcg gacggcgcag ggcagtgctg
     1141 attttttgat agtggcgcag cgcggcaaag gcggtttcgg cgatttcgtg gcggtcttgg
     1201 cggccgagct ttttatgttc gcggaaatag gcggagagga cggcatcggc aggctgtttg
     1261 aaagtcagca tttcagccaa aactttggcg gtatggtcga gttgtgcggc gttcataatg
     1321 taatgggttc tcttactgtg ggttattcat aatcaatcta acgtccttaa aataacaata
     1381 gcatctttat cattacttag tattctatcc gcaataagaa agatgtgtta aaagtgcgca
     1441 atcgggattt gtctaaattt ctccgttaac tacaaaggca gcctgaaaaa agttctattt
     1501 tcaggtcgct tgaacagcat ccgatataat cacatcagcg atttcatttt gtaatcactc
     1561 cccaaaacat gaggaaaaaa acaaaattta ttttcttcgt tcagtatatc tatcctaaat
     1621 atgccatcac tccattgagt ttttaatttg tatttcgctc ttgttaaatg tttttctgtt
     1681 tgccgatttc ttttgctaat gtaaaaaata ctctaccact ctcattggtt aaagttttca
     1741 agtattcatc aacatcagta acattaataa caactgtttc atttgtatgt ttattaaatt
     1801 ctaaatcaat aataatattc ccattaacca atatttcata tatatcttct tcttcaggga
     1861 gcatagacaa acaataagca cttgaagctg aaaactcttt tttcagatac tgaagaagaa
     1921 aagtattttt aattaaaata ttattaaatt tatctaatga ttgcttgact tctttagttg
     1981 tccaattttt aacattattc ttcattgtat tctacctggg tcgtagttat taaattacca
     2041 tacttatttg tttggatcgt cataatatta gttggcatgt tgttgttata tttatcatat
     2101 ccaatatcct tattaactcg gatgactctt tcataaattg tatgggaaat gagatttcca
     2161 tcactaagcc tctcaataga tatacgcgtt aatgttggga aagggtttca gacggccttc
     2221 ataatgcgtt cgatttcgtg ccaaaagccg tccggccgac attccaaaac ggcaatcaag
     2281 ccttggtcga tacggcggat ttcggtttcg ttcaatgttt cggtttcggc ggcacgcgca
     2341 ggtgcgaggt aggccatgcg gtcgagcaga tggtagcgga cttcttggcg ttcaaacccg
     2401 tattccgccc aatcttgaat atagacgtcg cgccagccgt atggattaag cggcggctca
     2461 aaagcatgga aaccttgcgg aaaaaatccg atgccgcgca cgatggaaac gggttttacg
     2521 tcaagcggca gaccgtgttg ccgcaaggtt cggatgccgt ctgaagtgtg cggcagaccg
     2581 agttgggcgg tcagtttggc ggcttttccc aacagcgtgt ctttggggtc gagtccgcgc
     2641 atgtcttcga gcctgcccgt gccgccgccg taatatttgc aggtcagctc gatatggtag
     2701 ggcttgccgt taagccttac cacaaaatcc gccgcgcctt gcgtattgcc gtccgaaccg
     2761 gaaaccgtga ggttgtgcgc gagcagttcg gcgtgtggcg cgttggtaaa ccaaaatgcc
     2821 agcagttctt cggcgtaaat gccgaggcgg tgtccgaacg gggcgcgtcg ggtgaggtgt
     2881 tccgtcagcg gcgcgggatc ggcatccaat gccaaaagat agcggaaacc gcgttccccc
     2941 aatagttcgc gcacgcccaa ttcgcaaccg ctttgccaca aaggcggcgc agtcagcagc
     3001 gaggcgaggt cgcggacggg ttggctggtg agcttccacc ataatgcgtc tagggcgtaa
     3061 ttcatgctta tccgaatatc agtcatgccg tctgaaaagc tggaaatgtt ttcagacggc
     3121 cttaatcagt cttttttatg gatacgatgg ctgacggtat tgaaaaaacc gcgcaggata
     3181 tcgatgtctt cggtttgcgt gttggcgcgt ccgaacaggc tctgcatacg gcgcatcaga
     3241 cgctcgccgt tgcggcggtt gaaaaagccg atgtcgttca tcacgctttc catgtgggcg
     3301 accatgcctt tgatttgctc gtgggtcgca gcgtggtctt cttgttgaag atgggtcatg
     3361 ggcgaaccgg tttggctgaa gatttcgtag cacacgacct gcacggcttg ggcgaggttg
     3421 agcgagaaat agtcgggatt gccgttgatg gtcatcagtc ggttgcaggc ttggacttct
     3481 tcgatgctca agccgaaagt ctcgttgccg aaaaccagtg ccactttctc gcctcggttt
     3541 gcggtctgca gtaattcgga taccaaatcg cgcggggttt gcagcggcgc agtaatttcg
     3601 cggcggcggc tggtcagggc gcaggcgatg gtggtgtcgg caagggcttc gtccaaagaa
     3661 gcggcaatgg tggcattttc caaaacgtct gccgcgccgg aagcgaggat gaagctttct
     3721 tccggtaatt taaacgattg aggatgctcc gggtcaaaca cgggcgggtt ttccgtcatc
     3781 ggcgttgcca tcagatttgg ggcgacgatg gtcagtttgt gcagacccat tgttttcatc
     3841 gcgcgcgcgg cagagccgat gttggcggga tggctggtgc gcgtgaggat aatgcggatg
     3901 ttgccgagat agtcgggcag ggcgggtttg agagtagtca tgttcggttt tcggtcggaa
     3961 tggaataggg tataatgcgc cgtctttttc gttcggacgg tcaaatgccg tctgaaatgt
     4021 tctttaacaa cgtcggaaat ttaacgttgc gcctgaagca gacacgcgtc gggcggtatt
     4081 ttacccgatt tggcaggatt taccccaacc aaataaactc cgattttgcc gctccgaagg
     4141 actgcacgtc cgaaccggcg gtatgccacc cttaaggaaa tctgatgaat ccgtttttga
     4201 atacagcctt taaagccgcc cgccgtgccg gtcagatgat gatacgcgcc gcgggcaatc
     4261 tcgatgccgt caaaaccgac agcaaagcat tcaacgattt tgtttccgat gttgaccgca
     4321 attccgaaat catcctggtt gaggctttga aagaagccta tccgcaccac aaaatcactt
     4381 gcgaagaaag cggctcccac ggtaaagccg cagccgaata cgaatggatt atcgatccgc
     4441 tcgacggcac gaccaacttc cttcacggcc atccccaata cgccatctct atggcactcc
     4501 tgcacaaagg cgtgttgcaa gaagctttgg tgtacgcccc cgaacgcaac gacgtttaca
     4561 tggcttcgcg cggcaaaggc gcgttgctca atgaccgccg catccgcgtt tccaaccgca
     4621 tcgaattgaa ccgctgcctg atcggtaccg gctttcctgt tgtcgatcaa agcatgatgg
     4681 acaagtatct ggcgattttg aaagatttct tggcaaaaac cgccggcggc cgtcgtgaag
     4741 gcgcggcttc tttggatttg tgtgctgtgg cgactggtcg ttttgacggc tttttcgagt
     4801 tcaacctcaa accttgggat attgccgcag gcgcattgat tgttcaggaa gcgggcggta
     4861 tcgtaaccga tatgacgggt gaagacggct ggctggaaag cggcgatgtt gtcgctgcca
     4921 atcccaaagt actggcgcaa atgttgaaaa tcatttccgc acacgtttaa atttcagccc
     4981 gctcccggga atgcgggaaa acgggcgggt atgccgtctg aagtatcgcg ccgatatttc
     5041 agacggcatt tttactggca gcgggggcag gaaggtggaa acggctaaat gcccgaaact
     5101 gccgaagatg gaaacccata tctgattaaa cggagaaccc tgtacgattt gtttatagtg
     5161 gattaacaaa aaccagtacg gtgttgcctc gccttagctc aaagagaacg attctctaag
     5221 gtgctgaagc accaagtgaa tgggcaaagc caacgccttt accgcaccga ttaccgtcgt
     5281 caattacgac gaacaagccc tcaataacac cgaagcgcgt actttggtgg atgccgtagc
     5341 gaaaaaagac gcttccgttt ggcagttcgg cggcgaaagc aacacgctga ccggtctgta
     5401 tttcagaggt tatcagcttg actcgcgcca attcagcgtc aacggtttgg caggtatgta
     5461 cggcacgcaa ggcacggcca gcgtgcaagt cggctcggca caactgatta aaggcgcgtc
     5521 caccgccgta aacggcatgg accctgaagg cgcggtatcc ggttccgtca atattgaaac
     5581 caaaaaagcc gccgacgaag acaaccgcaa aatcggtttg ggctggttta gcaacaaccg
     5641 cgcccaaggt acgttcgact tgggtcaacg cttcggcgaa aacaaagaat tcggcgtgcg
     5701 cgccaacggc aaactgcgcc atggcgacac cccgcgccac ggttacagcg aagacaacaa
     5761 agaatttgca ttgaatgctg attatcgcgg cgaaaaagtg cgcgtggcgt tcgactccat
     5821 ctacgcgaaa cgcaaaacca acggcggccg cgcgcgtatg caggatattc aaaacgccgg
     5881 cggccgcttg tttgacgcgc cagacggcaa aatcaacctg ctgctttcgt ggaactggca
     5941 aaacaccgtc ggcgaaacca atatgctgac ctttgaatgg gatgcgtttg acaatgccca
     6001 aattaccggc ggtatcggct acaacaaggc gcgttattac ggcacactga tttccccgac
     6061 cgtttgcggt acaagcggtg cgagcgacca aaccgctacc tgcacaagtg ccaagcaata
     6121 ccacaccggc acggcgagcc tgaccgacca atatttccgc acattgagta tgaacctgac
     6181 cgcacgcggc gaatttgaaa ccggcccggt aacacataac tggagtatgg ctttcgaccg
     6241 cgttatccgc cagcggaaaa ccataaacgg cagcgggaac ggcaacagca agatagaagt
     6301 gaaggcaaac gaaaatattg cgaatcaatt ggcatcgttt acagccgact atcccaattc
     6361 ttgggagaac tctgccaatt tggatgccaa tatcaaagtc aacagcctgg ctttgtccga
     6421 caccttgggc ttcgtggata acaaataccg cctgactttg ggcggacgtt tccaaacagt
     6481 cgaatatacc gacaaaaaat cacagagcgg cgatgccaaa cgtttcagcc cgatgctgat
     6541 ggcggcgtgg gtgccgcaac ctgatttggt tgtgtacggc aactatatgg aagacttgga
     6601 gcctgccgac atcaaaaccg atgattccgg cgaaacgaca atggcgaaac cgcgtgtcag
     6661 ccgccagttt gaagtgggtg tgcgtaaaaa ctggggcaat ttcgtaacca cgttaaacgc
     6721 gttccaaatc aaacgcccgg ctactggcgc ggtcatacaa ctaccaagac aacaaaaggg
     6781 gtaacaacaa cgctgaccta tggcaacaat tccgatttcg cccgctataa ggcgcaaggc
     6841 ggcgtagccg gcgacgagca aggtatggaa cgcaatcgcg gtatcgagtt caatgcctat
     6901 gctaacctgt taaacaaaac cctgcgtccg actttgggcc tgatgtatct gcaatcgacc
     6961 gtgaaagact acccaaattc acgcgatatg ctggttaacg gcgtacaagt cgccaatccg
     7021 cgcgtaattg ccaaggcagg tttggaatgg gatacaccgt ttgccaaagg cttgacttta
     7081 aacagcaatg tttcgtattt cggcaagtct taccaagaca cgcaaaaaca atacgccttc
     7141 ccatcctata ccttggttga tgtaggcgcg cgctacaaaa ccaagctcgg caaaaatacg
     7201 ttgaccgtca gcagctcggt ggaaaatctg ttcaacaaaa actactggca ggtacagcgc
     7261 ggccaatacg accgcagctt cgccgtcgtc ggtctgccgc gtacttactg gctgaaggcg
     7321 gaattggatt tctgatttct ccgcatctgg aaaatgccgt ccgaacaggt ttcagacggc
     7381 atttctttta aagagatatg gcgggatacg cttaaataat gctattctag cgtctttgaa
     7441 acctgcttgt ttgaagtgat gaaaaaacct ttgatttcgg ttgcggcagc attgctcggc
     7501 gttgctttgg gcacgcctta ttatttgggt gtcaaagccg aagaaagctt gacgcagcag
     7561 caaaaaatat tgcaggaaac gggcttcttg accgtcgaat cgcaccaata tgagcgcggc
     7621 tggtttacct ctatggaaac gacggtcatc cgtctgaaac ccgagttgct gaataatgcc
     7681 cgaaaatacc tgccggataa cctgaaaaca gtgttggaac agccggttac gctggttaac
     7741 catatcacgc acggcccttt tgccggcgga tttggcacgc aggcgcacat tgaaaccgag
     7801 ttcaaatacg cgcctgaaac ggaaaaagtt ttggaacgct tttttgggaa acaagctccg
     7861 gtttcccttg ccaataccgt ttattttaac ggcagcggta aaatggaagt cagtgttccc
     7921 gccttcgatt atgaagagct gtcgggcatc aggctgcatt gggaaggatt gacgggaaaa
     7981 acggtttatc aaaaaggttt caaaagctac cgcaacagct atgatgcgcc cttgttcaaa
     8041 atcaagctgg cagacaaagg cgatgccgcg tttgaaaaag cgcatttcga ttcggaaact
     8101 tcagacggta tcaatccgct tgctttgggc agcagtaatc tgaccttgga aaaattctcc
     8161 ttagaatgga aagagggtgt cgattacaac gtcaaattga acgaactggt caatctcgtt
     8221 accgatttgc agattggcgc gtttatcaat cccaacggca gcatcgcacc ttccaaaatc
     8281 gaagtcggca aactggcttt ttcaaccaag accggggaat caggatcatt tatcaacagt
     8341 gaagggcagt tccgttttga cacactggtt tacggcgatg aaaaatatgg cccgctggat
     8401 atccatatcg ctgccgaaca tctcgatgct tctgccttaa ccgtattgaa acgcaagttt
     8461 gcacaaattt ctgccaaaaa aatgactgaa gaacaaatcc gcaatgattt gattactgca
     8521 gtcaaaggcg aggcttccgg attatttacc cataacccgg tgctaaatat caaaactttc
     8581 cgtttcacgc tgccgtcggg aaaaatcgat gtgggcggaa aaatcatgtt taaagacatg
     8641 aagaaggaag atttgaacca attgggactg atgttgaaga aaaccgaggc agacatcaga
     8701 atgagtattc ctcaaaaaat gttggaagat ttggcggtaa gtcaggccgg aaatattttc
     8761 agtgtaaatg ccgaagatga ggcggaagcc agagcaagca ttgcagatat taatgaaaca
     8821 ttgcgcctga tggtggacag tacggttcaa agtatggcaa gggaaaaata tcttacttta
     8881 gacggtaatc agattgatac ggttatttcc cttaaaaaca acgccctgaa gttaaacggg
     8941 aaaacgctgc aaaatgaacc tgatcccgat tttgatgagg gcggtatggt ttcagagccg
     9001 cagcagtaat gggtcaaatg ccgtctgaaa tgttcaggcg gcattttgtt gtattggaaa
     9061 ctgccgtttt caaccattcc tttagataag ccgtgtcgga ttc
//
LOCUS       DAWHXZ010000066         9078 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000066 DAWHXZ010000000
VERSION     DAWHXZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9078
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.066"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(60..1616)
                     /gene="dnaA"
                     /locus_tag="WIE35_000990"
     CDS             complement(60..1616)
                     /gene="dnaA"
                     /locus_tag="WIE35_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ4153919.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     VSEGMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRQAAGSASRPESVA
                     VAKARTDVQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            1908..2042
                     /gene="rpmH"
                     /locus_tag="WIE35_000991"
     CDS             1908..2042
                     /gene="rpmH"
                     /locus_tag="WIE35_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ4153920.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            2045..2410
                     /locus_tag="WIE35_000992"
     CDS             2045..2410
                     /locus_tag="WIE35_000992"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ4153921.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            2475..2696
                     /gene="yidD"
                     /locus_tag="WIE35_000993"
     CDS             2475..2696
                     /gene="yidD"
                     /locus_tag="WIE35_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ4153922.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            2868..4505
                     /gene="yidC"
                     /locus_tag="WIE35_000994"
     CDS             2868..4505
                     /gene="yidC"
                     /locus_tag="WIE35_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ4153923.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKAEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMVAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRTQGEVVS"
     gene            complement(4586..5299)
                     /locus_tag="WIE35_000995"
     CDS             complement(4586..5299)
                     /locus_tag="WIE35_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ4153924.1"
                     /translation="MVSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHPETRLCTATDLTLPTQSVVSKTVADWRR
                     MKEMPNLKKRPTIFVMYAG"
     gene            complement(5358..5948)
                     /gene="maf"
                     /locus_tag="WIE35_000996"
     CDS             complement(5358..5948)
                     /gene="maf"
                     /locus_tag="WIE35_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ4153925.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAHKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            6096..6599
                     /locus_tag="WIE35_000997"
     CDS             6096..6599
                     /locus_tag="WIE35_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ4153926.1"
                     /translation="MSDPNLIDSEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            6633..6812
                     /gene="rpmF"
                     /locus_tag="WIE35_000998"
     CDS             6633..6812
                     /gene="rpmF"
                     /locus_tag="WIE35_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ4153927.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            6987..7514
                     /locus_tag="WIE35_000999"
     CDS             6987..7514
                     /locus_tag="WIE35_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ4153928.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            7602..8657
                     /gene="plsX"
                     /locus_tag="WIE35_001000"
     CDS             7602..8657
                     /gene="plsX"
                     /locus_tag="WIE35_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ4153929.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            8793..9044
                     /locus_tag="WIE35_001001"
     CDS             8793..9044
                     /locus_tag="WIE35_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ4153930.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
BASE COUNT         2353 a         2771 c         2085 g         1869 t
ORIGIN      
        1 ctatatcttt acccttcgga cggcatgggc aatatcatgt cgtctgaaaa cgttttccat
       61 cagttttgaa tcagaatcag cagcttttca taatcctgag ccaattccgg atcttcttcg
      121 cgcagtttcg ccactgccct gatgccgtgc ataacggtcg tatggtcgcg cccaccaaac
      181 gaatcgccga tagacggcag gctcaaagta gtcagttctt tggtcaggct catcgccacc
      241 tggcgcggac gggcaatgtt tcgtgtccgt ttcttaccga gcacatcgct gattttgatg
      301 cggtaatatt tcgccaccgc atcgatgatg atgtcggcgg tgatgacttt gtgcttctcg
      361 gcaataatgt cctgcaaagc ggtacgcgcc aaatcgatgt cgatgacggg acggttcata
      421 aagcggctgc tcgctccgac acgattaaac gcgccttcaa gctcgcgcac gttggaacgg
      481 atcagattgg caatgaacag cgcggcttcg tcttcgatac tgatgcccgc cgcttccgcc
      541 tttttctgca aaatggcgat gcgcatttcc aattcgggcg gctcgagttc caaagtcagt
      601 ccccatgaaa aacgggattt gaggcggtcg tccatgcctt cgattttcgc aggcaacaca
      661 tcgcaagtga ggatgagctg ttttttctcg ttgtggaaat ggttgtacag atagaaaaac
      721 tcttccatcg tacggtcttt gcctttgatg aactggatgt cgtcgataat cagcaggtcg
      781 tattgcttgt attgctgctt gaacacgtcg taagtgttgt tgcgaaccgc cttcataaag
      841 ctgcggatat agtcatccga atgcatatag cgcactttgg catcgggacg gtttttcagc
      901 agctcgttgc cgaccgcctg cacaaggtgg gttttgccca aacccgtact gccatagagg
      961 aagaacgggt tgtaactctg ccccgggctt tccgcaatcg cctgcgccgc agccgccgca
     1021 aggcggttgc ccttaccttc taccaacgta tcaaacgtgt aatccggaga caggttggtc
     1081 tgctcgtaac gcgcctcttc cgcatcgcgc tgcacgtccg tccgtgcttt ggcaactgcc
     1141 accgattccg gccgggaagc agacccggca gcctgacgcg gctcgtgcgg caggtttttc
     1201 atacgttccg ccaaaatatc cgccgccgtt ttcgacgcag cgggtttgac aggctcttca
     1261 gacggcagct cgtccaacag aacctcctgc acgggcattc cctccgacac cgcatgcaag
     1321 gacggctcgg caggttcgac agcaccttca accgccgcca tctcataacg cacgccttct
     1381 cccggtttga atacgaaggc ggaacggccg gcagccaact cttccctcac cgcttctatt
     1441 tttccggcaa actggctctt gagcatattg caggcaaact ggttcttgcc gtacaccacc
     1501 catacgccac cctcctcacc aacggtaagg ggcgcaatcc attgcgcaaa ctgcccttga
     1561 ggcaacatat cgtgaagacg gcggaggcac agcggccaaa actctgctaa tgtcatggat
     1621 aagctcgaat cggtaaaaat gaaatcgaaa acaaagaaaa tataatattt tcaaaaagaa
     1681 aacaaatctg ttgaacgcac atcggttcaa aacgcgactg cccgattata ccgactcacg
     1741 aatattttat ccacaacccg tgcaaaaatt tatccacaga aaggcggcgg aaatccgcag
     1801 gcaatcgggc aatcttcctg caaagtttct atattgattg acaaaagcgg caaattggag
     1861 tgtaattcac ggtttaatta tctacccatt ctattttagg aaacatcatg aaacgcactt
     1921 atcaaccttc cgttaccaaa cgcaaacgca cccacggctt cctggtgcgc tccaaaacgc
     1981 gcggcggccg cgcagtattg gccgcacgcc gtgccaaagg ccgcaaacgc ctggcggtat
     2041 aattttggac taccgcttcg gaaggcagta ccgcttgttg aaaacggatg atttttcatc
     2101 cgtttttgca ttcagaaacc gccgcagccg cgacctgctg caagtttcgc gctcaaacgg
     2161 caacgggctg ggccatcccc gcatcggtct ggtggtcggc aaaaaaaccg ccaaacgcgc
     2221 caacgaacga aattatatga agcgcgttat ccgcgactgg tttagattga acaaaaaccg
     2281 gctgccgccg caggatttcg tcgtgcgcgt ccaccgtaaa ttcgacaggg ctaccgcaaa
     2341 acaggcaagg gcggaactgg cacaactcat gttcggcaac cccgcaaccg gatgcaggaa
     2401 acaggcatga tcagaacggt actctgcagg caaggttcag acggcaacgg gtttcccata
     2461 caaggaacat cccgatgaac ttcctattgt ccaaactcct gctgggactg atacggttct
     2521 accaatattg catcagcccg ctgattccgc cgcgctgccg ttatacgccg acctgttcgc
     2581 aatacgcggt cgaagcggtc aaaaaatacg gcgcattcaa aggcggccgg ctcgccatca
     2641 agcgcattgc acgctgccac cctttcggcg gacacggaca cgaccccgtt ccctgacccg
     2701 acgcaatatt caaattgcac gctttccttt tatttcccat cggtttctat ataatgccgt
     2761 ctgaagcttc gggcaggcgg cacgaccgcc gggtatgaag cccgccctta ttccccgtct
     2821 atcggaacac gcaacctgcg gcatttccga ccattcagga aactcttatg gattttaaaa
     2881 gactcacggc gtttttcgcc atcgcgctgg tgattatgat cggctgggaa aagatgttcc
     2941 ccactccgaa gcccgtcccc gcgccccaac aggcagcaca acaacaggcc gtaaccgctt
     3001 ccgccgaagc cgcgctcgcg cccgcaacgc cgattaccgt aacgaccgac acggttcaag
     3061 ccgtcattga tgaaaaaagc ggcgacctgc gccggctgac cctgctcaaa tacaaagcaa
     3121 ccggcgacga aaataaaccg ttcatcctgt ttggcgacgg caaagaatac acctacgtcg
     3181 cccaatccga acttttggac gcgcagggca acaacattct aaaaggcatc ggctttagcg
     3241 caccgaaaaa acagtacagc ttggaaggcg acaaagttga agtccgcctg agcgcgcctg
     3301 aaacacgcgg tctgaaaatc gacaaagttt atactttcac caaaggcagc tatctggtca
     3361 acgtccgctt cgacatcgcc aacggcagcg gtcaaaccgc caacctgagc gcggactacc
     3421 gcatcgtccg cgaccacagc gaacccgagg gtcaaggcta ctttacccac tcttacgtcg
     3481 gccctgttgt ttatacccct gaaggcaact tccaaaaagt cagcttctcc gacttggacg
     3541 acgatgccaa atccggcaaa tccgaggccg aatacatccg caaaaccccg accggctggc
     3601 tcggcatgat tgaacaccac ttcatgtcca cctggatcct ccaacccaaa ggcggacaaa
     3661 gcgtttgcgc cgctggcgac tgccgtatcg acatcaaacg ccgcaacgac aagctgtaca
     3721 gcaccagcgt cagcgtgcct ttagccgcca tccaaaacgg cgcgaaagcc gaagcctcca
     3781 tcaacctcta cgccggccca cagaccacat ccgttatcgc aaacatcgcc gacaacctgc
     3841 aactggccaa agactacggc aaagtacact ggttcgcttc cccgctcttc tggctcctga
     3901 accaactgca caacatcatc ggcaactggg gctgggcgat tatcgtttta accatcatcg
     3961 tcaaagccgt actgtatcca ttgaccaacg cgtcctaccg ttcgatggcg aaaatgcgtg
     4021 ccgccgcgcc caaactgcag gcaatcaaag agaaatacgg cgatgaccgc atggcgcaac
     4081 aacaggcgat gatgcagctt tacacagacg agaaaatcaa cccgctgggc ggctgcctgc
     4141 ctatgctgtt gcaaatcccc gtcttcatcg gattgtattg ggcattgttc gcctccgtag
     4201 aattgcgcca ggcaccttgg ctgggttgga ttaccgacct cagccgcgcc gacccctact
     4261 acatcctgcc catcattatg gcggcaacga tggtcgccca aacttatctg aacccgccgc
     4321 cgaccgaccc gatgcaggcg aaaatgatga aaatcatgcc gttggttttc tccgtcatgt
     4381 tcttcttctt ccctgccggt ctggtattgt actgggtagt caacaacctc ctgaccatcg
     4441 cccagcaatg gcacatcaac cgcagcatcg aaaaacaacg cacccaaggc gaagtcgttt
     4501 cctaaatgcc gcagcatgaa aaatgccgtc tgaaacctgt tcagacggca tttttattgt
     4561 tcacccccta tcggggcgga aatcttcaac ctgcatacat cacaaaaatc gtcgggcgtt
     4621 ttttcagatt gggcatttcc ttcatcctgc gccaatccgc aaccgtttta ctgaccaccg
     4681 actgagtcgg caacgtcaaa tccgtagccg tgcacaagcg cgtttcagga tgcaggtttt
     4741 ccaccgcatc cgccaacaac acatcgttgc gatacggcgt ttcgataaac agctgcgttt
     4801 cattctgctg gcgcgaacgt tgttccaaag ccttcaaact ctgaatccgc tcattttttt
     4861 cagacggcaa ataaccctta aacgcaaagt tctgcccatt cgcacccgaa gccatcagcg
     4921 ccagcaacaa gctggacgga ccgaccaaag gccgtacttc aaaaccgtgt ttatgcgcca
     4981 atgccaccaa attcgcaccc ggatcagcca cagccgggca acccgcctcg ctgacaatgc
     5041 ccatactgcg cccttcttgc aaaggtttca gcaattccgg caaagtcttc aaatccgtgt
     5101 gttcgttcag cgtttgcaaa ttcagttcgc ggataggcat tgtcacgccc aaatgtttca
     5161 aatgcgcgcg cgccgttttt tccgcttcca cgacaaaatc cgtcagcccg acaatcgcct
     5221 gttgttcatg cggcaacagg cacggcgtgt caggcgtacc caaaggcgta ggaatcaaat
     5281 acaaaacagg agacaccatt ccctcactca tcggttaaaa atgccgtctg aacccttcag
     5341 acggcataaa cgggcagtta cagaacctcc acgccctcat ttttcaaaaa atcaaccaga
     5401 cggaaaaccg gcaaaccgat taaagcattc ggatcggtac tctcaatcct ttcgatcaac
     5461 aatgcaccca aagcctcgct cttcaacgca cacgagcaat aaaccgcatc aggctcgcgc
     5521 tccaaatagc ggaggatgtg tagctcgtcc aacttcctca tcacgaccac cgtcttatcg
     5581 atatgccgct gcatcctgcc cgtaaccgta tttagcagaa cgaccgcgct gtaaaactca
     5641 atctccctgc cgctcaaatg catcaacatc ttgtgcgcat tagcaaggtt catcggcttg
     5701 ccccactgcc tgccgtcgca ccacgccacc tggtccgcac cgacaatcaa cgcctctggg
     5761 aaacgcccgg ccaacgaccg cgccttaccc tcggcaaggc gcaatgccgt ctgaggggcg
     5821 gattccccca acatcggcgt ttcgtcaaaa tcgggggacg ccgcctgaaa ggcaatgccg
     5881 agcctttcca tctgttcgcg gcggaaaacc gaactcgtac ccaaaatcaa aggcagttcc
     5941 aaacccatcc catcctcctt accgttgaaa acacgcccga aggggcagta aaatccagcc
     6001 atgcgccgaa acacggatac ccgccttcgg cgtaccgcaa catttttctt aaaaatattg
     6061 acgttagaac atctaaatta tatcatatcc cgtttatgtc agaccctaat ttgattgact
     6121 cggaaatttt tgccgccgaa gggcagaacc tgcaaggcag ttttctgctg gaagaattgg
     6181 atgaacgcgt cagttcgcac gattatcccg ccgacaggca gaccaaaata tcgtttacac
     6241 tgaccggcgg tcgcgaccgg ctgcaacgcc tgttcctcga cctgaacgtc aaagccgata
     6301 tgcccctgat ttgccagaga tgtatcaaac ccatgccgtt catgcttgat gaaagcagcc
     6361 gtatcgtcct gttttccaac gaagagtcct tggacgaatc catgcttgcc gacgaagaac
     6421 tcgaaggcat actgattgaa aaagaactcg acgtgcgcac attggtagaa gaccaaatcc
     6481 tgatgtccct gcccttttcg ccgcgacacg aagactgcgg cgacaatggg acactggaag
     6541 aagtcaatcg ggacaaaccc aacccctttg ctgttttggc aggtttgaaa agcaattgat
     6601 taggacacag tttatttatc taggagcttg aaatggccgt tcaacaaaac aaaaaatccc
     6661 cttccaaacg cggtatgcac cgttcgcacg acgcgctgac cgcgcccgca ctgtctgtcg
     6721 acagcacaac cggcgaagta caccgcccgc accacatttc ccccaacggt atgtaccgcg
     6781 gccgcaaagt ggtcaaagcc aaaggcgaat aatccctatt cgactgactg aaaaagccag
     6841 aacattgcca tgcaattact ggcttttttt gcattggacg caccatccgt ccaaactttc
     6901 gccatacgtc aacacacagg ggcaaagcgt tccgtataat aacccgtgaa aatattccaa
     6961 aagccccaac caccaaggaa attccgatga aacagaaaat ctggtacacc tacgatgaca
     7021 tccaccgcgt catcaaagca ttggcagaaa aaatccggaa cgccgacatc aaatacgatg
     7081 ccatgattgc catcggcggc ggcggcttta ttccggcacg tatgctgcgc tgttttctgg
     7141 aaattccgat ttatgccgta accaccgcct attacgacag cgacaacgaa ggacaggtta
     7201 cagaagaagt caaaaaagtc caatggctcg accccgttcc cgaagccctg cggggcaaaa
     7261 acgtactcgt cgtcgatgaa gtggacgaca gccgcgtaac catggaattt tgcctgacag
     7321 agctgctcaa agaagacttc ggtacgatcg gcgtcgccgt actgcacgaa aaaatcaaag
     7381 ccaaagcagg caaaatcccc gaaggcattc cctatttcag cggcatcacc gtagaagact
     7441 ggtggatcaa ctatccgtgg gacgcactcg acatcgacga acacaaccgc cttgccgagg
     7501 ccggccgagg ctgacccttt cagacggcat attttccgaa ccgatgccgt ctgaagcccg
     7561 cacgacccct gccgcagacc gaaaacctac cggagaaccc tatgattaca cttgccgtag
     7621 atgccatggg cggcgaccaa ggacttgccg ttaccgtacc cggcgcaacc gcattcctcc
     7681 aagcacaccc cgatgtccgc ctgattatga ccggcgacga aacgcaactg cgccaagccc
     7741 tgaccgcggc aggcgcaccg atggaacgca tcgacatctg ccataccacc caagttgtcg
     7801 gcatggatga atcccctcag tccgccctaa agaacaaaaa agactcctcc atgcgcgttg
     7861 ccatcaacca agttaaagaa ggcaaagccc aagccgccgt atccgcaggc aacacgggcg
     7921 cactcatggc aaccgcacgt ttcgtcctca aaaccattcc cggcatcgaa cgccccgcca
     7981 tcgccaaatt ccttccttcc gacaccgacc acgttaccct cgccctcgac ctcggcgcaa
     8041 acgtcgactg cacgcccgaa cagctcgccc aatttgccgt tatcggcagc gaactcgtcc
     8101 acgcactcca tcctcaaaaa ggacagccgc gtgtcgggct ggtcaacgtc ggcacggaag
     8161 acatcaaagg tacggacacc gtcaaacaaa cctacaaact gctgcaaaac agcaaactca
     8221 actttatcgg caacatcgaa agcaacggca tcctgtacgg cgaagcagat gtcgtcgtcg
     8281 ccgacggctt tgtcggcaac gtcatgctca aaaccatcga aggcgcggtc aaattcatga
     8341 gcggagccat ccgccgcgaa ttccaaagca acctgttcaa caaacttgcc gccgttgccg
     8401 ccctacccgc cctcaaaggg ctgaaaaaca aactcgatcc gcgcaaattc aacggggcca
     8461 tcctgctcgg gctgcgcggc atcgtcatca aaagccacgg cggcacagac gaaaccggtt
     8521 tccgctacgc actcgaagaa gcctaccacg aagccaagtc cgccggcctt tccaaaatcg
     8581 aacaaggcgt tgccgaacaa ctcgccgcac tcgaaactgc caaagccgtc caaaacgaaa
     8641 atgccgacgg tctgtaacac acacgatgcc gtctgaacgc ccccgcccct ttcagacggc
     8701 atccgcccgc accaaacctg cgggcgcgga cggcgatgcg cctgtccggc acttcccaaa
     8761 tacttccctg taaaataagg agtatttgaa aaatgaagac attagaaaaa cggatgaaag
     8821 ctctagacaa acagatgatg aagttcggaa aatcccttga aggcaggctt gatgcccgtc
     8881 tgattgaatc cgcattggat tatattcatt attcggaacg ttttttggct tttgaaatcc
     8941 tgtgtactta tatcgaagat ttcgatgtcc ggctgacgga acaagaatcc cgggaaattt
     9001 cttttatcaa caaggaattt gagatagaaa gcacgtccga ttaaccaata aagccaatgg
     9061 gttgataaac atgaaaac
//
LOCUS       DAWHXZ010000067         9018 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000067 DAWHXZ010000000
VERSION     DAWHXZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.067"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(53..361)
                     /locus_tag="WIE35_001002"
     CDS             complement(53..361)
                     /locus_tag="WIE35_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153931.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHNHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="WIE35_001003"
     CDS             complement(345..647)
                     /locus_tag="WIE35_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ4153932.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /locus_tag="WIE35_001004"
     CDS             complement(721..3084)
                     /locus_tag="WIE35_001004"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ4153933.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQIDAPADCGRFI
                     SRVIENVNARATTPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGNVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3120..3638)
                     /locus_tag="WIE35_001005"
     CDS             complement(3120..3638)
                     /locus_tag="WIE35_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153934.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3645..4709)
                     /locus_tag="WIE35_001006"
     CDS             complement(3645..4709)
                     /locus_tag="WIE35_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ4153935.1"
                     /translation="MIKSFELENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WIE35_001007"
     CDS             complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WIE35_001007"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ4153936.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="WIE35_001008"
     CDS             complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="WIE35_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ4153937.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="WIE35_001009"
     CDS             complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="WIE35_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ4153938.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(6940..7461)
                     /gene="infC"
                     /locus_tag="WIE35_001010"
     CDS             complement(6940..7461)
                     /gene="infC"
                     /locus_tag="WIE35_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ4153939.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(7479..>9018)
                     /gene="thrS"
                     /locus_tag="WIE35_001011"
     CDS             complement(7479..>9018)
                     /gene="thrS"
                     /locus_tag="WIE35_001011"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ4153940.1"
                     /translation="ELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLIDDMPEVEAM
                     GMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIYGTAWATKDE
                     LKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVIEQHMRKELN
                     AAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCPGHVQIFNNG
                     LRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQIVSEARAFNE
                     LLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVEWGELPGEGA
                     FYGPKIEYHVRDALGRSWQCGTLQLDFVLPERLGAEYVTENNDRSRPVMLHRAILGSL
                     ERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGFRAELDLRNE
                     KIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIAQLQQEITDA
                     LVNH"
BASE COUNT         2246 a         2317 c         1933 g         2522 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacaggtctt tcagaccgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgattccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgtg attatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta ggattacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgaccgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccgtgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacgg cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgacgttgcc
     1141 gtctgaaacg atattggcgg cgcgtccggg atgcagggca ggatgtccgg ttttaacgaa
     1201 ctcgaccgct ttgtttttca acagattttc cacatctgcc ttaatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccgcccc attgttcggg cattgccgcg ccgtaccaca agccgccgat
     1321 gcgttcgttt tggacaaacc ggccgtctga acctttgccg aacacgcggg cgatttcaaa
     1381 cacgcgcacg cggttttgtt tgcggttcag gttgttttgc aaaatttcca ccaagccgcc
     1441 gatgagtgtg gaacgcatca cggcatattg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtag ccgcgcgccg ccatttcgtt gtaaacggca aaacgcgggc ggcgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc agcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcaggccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggcccaa
     1861 acgcaatccg acctgcttcg cttcgggcaa ttcgccttgc gcttccacca tttcgcctgc
     1921 cgcaccgccg caaatctgca acaccaattc ggtagcacgc tcaatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acacgccttt
     2161 ttcgtccgcc acgaccagcg tgttttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 aaaaacgtgc atcggctgac cgatttccag catcacataa ttgccgatgt ccaccagcgc
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatccg gcgtagtagc
     2401 gcgcgcgttc acgttttcaa tcacacggct gataaagcga ccgcaatcgg caggcgcgtc
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cctttgatgc tcaagcagtc
     2581 ggcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcgcg
     2641 gatattggta ccgacgggcg catcttcagg cagaatgtgc aggccgttca caccgtcgtc
     2701 gggcagaccg agttcgtcgg tggaacacaa catcccgtcc gacaccacgc cgcgcatttt
     2761 ggtcggcttg attttgaaat tgcccggcaa cactgcgccc ggcaacgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacaatctgc accaacccgc ccgtacccgc
     2881 atcaactcgg gtaacgttca aacggtctgc atccggatgt ttttcaacgg atttcacttc
     2941 ggcaatcacc acgcccgcaa acgcaggcgc ggcagtttcg gcctcttcca cttccaagcc
     3001 ggacatcgtt aacagatgtt ccagcttatc ggaggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgaa taggagaatt gcatgttatg ttctcaatca attaattaac tttagatttt
     3121 caagagctag ctaaaccttt ttgaatggct aagtccctat taattacttc tacagaaatg
     3181 ttccataaag aaatcaaccg caaagtatct ttcttattaa attcttgctt tattaattca
     3241 ttcaattgcc caagataatt aagcggcaca tccgggataa ccagtaataa tttgatttct
     3301 atgctacccc attgcaaatt tgaaaaagca ctaggcataa ttgtttcttt gatatcttct
     3361 ctgcctaata ttcccaaaat ttcaacattt aatgcattac accatttttg ataaatctct
     3421 gaaatatatt catcaaaacg ctcaggagat ctttcttgat ttggaatact ttgttttgcc
     3481 tcaacaaata acaaattatc ttcttgcaga tagataaact ctactgtctt aataccttct
     3541 tgccttctct tccggatata tggttctata taaaacaggt tattttttac tagattaaaa
     3601 gttatacctg attcagtgag atttattatt tcactcataa aaacctaacc aatccatgct
     3661 gtttccggag ataaaacacc tatttcttct ctgaacaatc gaatagactc accaataatt
     3721 tcattatccg gcatgctatt ttgtaaattt tcctgcaccc aagtagtctg ttccttttct
     3781 ttgccaggca taaaaacagg aatactttca ctatgctttc tagctaataa acacagcttc
     3841 ttgataacaa aatatgaatg cgtactcata aaaatctgta ttcccaattt ggatttagat
     3901 aaggaaaaca gaatttctag aaattgatta attgctttcg gatgtagatt agcttctggc
     3961 tcatcaataa ataagattga tccaggactc aaaaaccgat ttcctaatag agtgtctaat
     4021 atcccaatct tttttacccc ctctgctgta gaattgatag agaaagtttg tctatctttt
     4081 tcatataccc atgccttctt tttatcattg tagctaacat aaccgccaaa caatgcttct
     4141 agatcctttc tagcttccgc tgcctcaggg taattcctcc cttttgttgg tttaatatcc
     4201 aatgctttta ccaaatcaat gtaagtagca tcaaaaccaa atctcttttc ttcctctgat
     4261 tttttaatta catcaaatag tgatagaact tctttgggtg gtaaaaaaat agagttagcg
     4321 tttattctgg gagaaagttc gtatagctcc ggtttaatta gctttttggt agttctacca
     4381 aaagaaaaca aaactgaaga actatcttct aaagtcatac ttaacttaag aggccgttcg
     4441 tttggatttt cttttcctct tgtaactaaa tcaccaattt cctctacttg gaacgtccaa
     4501 tatagcttat ctcgcaattc ctcagaaaaa tcacgaatat tctttccttt atgagcttcc
     4561 tcatgacttt ttaaaacact atataatgcc tttaaaataa aagttttccc tttgctattt
     4621 tctgcaataa ttaaatttat tttccctaaa ttatcagcct tcaataagct aatcggcccg
     4681 aaattttcaa gctcaaatga tttaatcata taatccccct aatatatatc aacaatattt
     4741 gtacaagcca cattgtaaaa agacagcaac cacagcacca tagtaaacgc atagcaggcg
     4801 gataatggag cgacatagcg tttcatgatt tagctttctg aggtaggcac aggctgcttt
     4861 tttcattttt aggcagccta cacaatcatt ttacaaactg cttcaaaaaa ttcaaatcgt
     4921 tatcaaaaaa caagcgcaaa tcgttcacgt tgtaacgcag catcgcgaaa cggtcgagac
     4981 cgataccgaa agcgaaaccg gtgtattttt cagaatcgat attcacattt ttcaacacgt
     5041 tgggatgcac catgccgcag ccgccgactt ccagccattt gccgttttct cccataatgt
     5101 cgatttcggc agacggttcg gtgaacggga agaaagacgg gcggaaacgt acttgcaaat
     5161 catcgcgttc aaagaagcga cggataaaat ccgtgaacac tgcttttaag tcggcaaaag
     5221 ttacgccctc ttctacccac aaaccttccg cctgatggaa cataggcgag tgcgtggcat
     5281 cgctgtccac acggtaaacg cggccggggg cgataatgcg gatgggcggc tcttttttat
     5341 cgagcatata gcggatttga atcggggaag tgtgcgtacg caaaacatca ccgttttcaa
     5401 cgtaaaacgt atcctgcatc gcacgggcag gatggtttgc agggatgttc agggcttgga
     5461 aattgtgaaa atcgtcttcg atttcaggcc cgtccgccac ttcgaaaccc attccgtgaa
     5521 agagttcgac cacacgttgc aaggtcaggg ttacgggatg caggctgccg ccttcctgag
     5581 cgcgtccggg cagggtaatg tcgagggctt cggcggcaag tcgggcttgc agcttggctt
     5641 cgttgagggc atcgcgtttg gcattaaaag ccgtctgaaa ccggtttttg cattcattga
     5701 tatgcgcacc tatggttttg cgctcttcag gcgacatttg ccccaaagtt ttcagaagtc
     5761 cggtcaactc gccggttttg ccaagataac gggctttgat ttgttctaga gcgttgaagt
     5821 cttgcgcagc ttctactgcg gcaatgcctt ctgcaacgat gcggtttaca ttttccataa
     5881 tatcaagtct gtcaattaat gatatgccac ctgaaacaac gaggccgcct gtatagcctg
     5941 acatatcaga ctcttcagac gaccttattt tcttcacggt caatccattg gataaatttt
     6001 ctgtatagtg gattaaattt aaaccagtac ggcgttgcct cgccttgccg tactatctgt
     6061 actgtctgcg gcttcgtcgc cttgtcctga tttaaattta atccactata ttttaacgca
     6121 aatttattta aaaaaataga ctttaaagaa agaaaatcaa tcacatagaa tttctgcaaa
     6181 caaagaaaaa aggaagccgc agcttccttt tcaatttttt ggattaagca gccaaagcag
     6241 ctttggcttt ttcaaccaat tgtgcaaaag cggctttatc gaacacggcc aaatcagcca
     6301 atactttgcg gtcgatttcg atagaggcgc gtttcagacc attcataaat ttgctgtaag
     6361 acaacccgtt ttcacgcgca cctgcattga tacggacaat ccacaattga cggaattggc
     6421 gtttgcgttg gcggcggtcg cggtatgcgt attggccggc tttcattacc gcctgcttgg
     6481 caacgcggta aacgttttta cgacggccgc ggtagccttt ggctaacgcg aagatttttt
     6541 ggtgacgggc acgggcggta acaccgcgtt ttacgcgtgg catattctaa actccttaag
     6601 cgtagggtaa cattttagca acagaagcca aatcgcgatc atttaccata gaggtaccgc
     6661 gcagttggcg tttgtttttg gtggtctttt tagtcaagat gtggcgtttg aacgcatgag
     6721 cgcgtttcac accgccgtta cccagtactt taaagcgttt tttcgcgcta gacttggttt
     6781 tcattttagg catgggaaaa ctccattcgt tatcagataa ggcattaggg ggttttaaaa
     6841 catcggtttc aaacacttga acccacaata ccacggtttt ttgccgcaat tgaaaactta
     6901 ctccgaagcg gcaatcggag taagcggaga attatagctt tattttttct tcggcgcaat
     6961 catcatcacc atttggcgac cttccatttt gggaaaggac tcgatttgcg ccacttcagc
     7021 caaatcttct ttcacacgtt ccaaaagttg cgcgccgagt tgctggtgag ccatttcacg
     7081 gccgcggaaa cgcaatgtca ctttgacttt atcgccgtcg gcaaggaagc ggttgatgtt
     7141 gcgcatcttg atttgataat cgccctcatc cgtacccgga cggaatttga tttccttgat
     7201 ttgcacctgc ttttggtttt tcttggcttc gtcgcgtttc ttggcctgct ggtatttgta
     7261 tttaccgtaa tccatcagtt tgcacacagg cggtttagca gttggggaaa tctctaccaa
     7321 atcgacatcc tgcccttcgg ccatagccaa agcttcacga actgaaacaa caccaagctg
     7381 ttcgcctgac tcactgatta aacgcacttc tttggcggta atttcgccgt tgattcgtgc
     7441 ttcgcgttct tgagcgatga tgaatactcc tataaaaatt aatgattgac gagggcatca
     7501 gtgatttctt gctgcaattg cgcaatgaaa tcatccaaat ccaaagaacc caaatcttct
     7561 gctttgcggc gtaccgccac tttgttttcc tgcttctcct tatcgccgac aacgatttga
     7621 taagggaaac ggtattggct gttgtcgcgg attttgtaac cgattttttc gttacgcaaa
     7681 tccaactcgg cgcggaatcc tgccgcctgc aatttggcag ccacttcccg acaataatct
     7741 gcctgatttt cggtaatatt cataatcacc atttgaaccg gagccaacca taacgggaaa
     7801 gagcctgcat ggttctcaat cagaatgccg ataaatcgct ccaaagaacc taaaatggcg
     7861 cgatgcaaca taacaggtcg cgagcggtcg ttgttttcgg ttacatattc tgcacccaag
     7921 cgttccggca agacgaaatc cagttgtaat gtaccgcatt gccaagaacg acccaaggca
     7981 tctctgacat gatattcgat tttaggcccg taaaacgcac cctcgcccgg caattcgccc
     8041 cattccacgc cgcaggcagt caatgcctcg cgcaaaccct gctctgcctt atcccacacg
     8101 tcatctgaac ctgcacgttt ttcagggcga agagaaagct tgacggatac atcatggaaa
     8161 ccgaactgtt tgtagatgcg aaccaacaat tcattgaacg cacgggcctc gctgacgatt
     8221 tggtcttcgg tacagaaaat atgcgcatca tcctgcacaa atccacggac ccgcatcaga
     8281 ccatgcaatg caccgctcgg ctcgttgcgg tggcaggaac cgaattccgc caaacgcatc
     8341 ggcaaatctc gatacgaacg caaaccgttg ttaaaaattt gaacatgacc cggacagttc
     8401 atcggtttaa ccgcatattc gcgtttttcc gaactggtta cgaacatatt atctttgtag
     8461 ttgtcccaat ggccggattt ttcccaaaag gttttatcca tgatttgagg cgttttgacc
     8521 tctttataac cggcggcgtt cagttcttta cgcatatgct gttcaatcac ttgccacaaa
     8581 gcccagcctt taggatgcca aaacaccatg cccggcgctt cgtcttgcag atggaacaga
     8641 tccaattgct tgccaagctt gcggtggtcg cgcttttcgg cttcttcgat acgttgaata
     8701 taggctttca actcgtcttt tgtcgcccaa gccgtaccgt atatgcgttg cagcatttca
     8761 ttattgctgt cgccgcgcca gtatgcgccc gccagcttgg tcagtttgaa gtttttcagg
     8821 aaacgggtat tcggaacgtg cggaccgcgg cacatatcga catattcctg gtgatgatac
     8881 atccccatcg cttccacttc gggcatatcg tcaatcaggc gcagtttgta ttcttcgccg
     8941 cgctcttgaa aaattttaat cgcctccgca cgcggagtca tgattttgac cacatcatag
     9001 tcttgggcaa tcaattct
//
LOCUS       DAWHXZ010000068         8987 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000068 DAWHXZ010000000
VERSION     DAWHXZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8987
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.068"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(240..1505)
                     /gene="ftsY"
                     /locus_tag="WIE35_001012"
     CDS             complement(240..1505)
                     /gene="ftsY"
                     /locus_tag="WIE35_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ4153941.1"
                     /translation="MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEEVE
                     SLTESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAVAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
     gene            1651..3219
                     /gene="msrAB"
                     /locus_tag="WIE35_001013"
     CDS             1651..3219
                     /gene="msrAB"
                     /locus_tag="WIE35_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ4153942.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            complement(3321..3806)
                     /locus_tag="WIE35_001014"
     CDS             complement(3321..3806)
                     /locus_tag="WIE35_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ4153943.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLAAVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(3832..4680)
                     /locus_tag="WIE35_001015"
     CDS             complement(3832..4680)
                     /locus_tag="WIE35_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ4153944.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            complement(4774..5775)
                     /locus_tag="WIE35_001016"
     CDS             complement(4774..5775)
                     /locus_tag="WIE35_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4153945.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPELAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            5834..6565
                     /locus_tag="WIE35_001017"
     CDS             5834..6565
                     /locus_tag="WIE35_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ4153946.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGLFTLINRAMTRYMTRRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDEVLTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTTPRKYERMLCVKPSLS"
     gene            6541..6813
                     /locus_tag="WIE35_001018"
     CDS             6541..6813
                     /locus_tag="WIE35_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ4153947.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPIYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            6868..8238
                     /gene="glmU"
                     /locus_tag="WIE35_001019"
     CDS             6868..8238
                     /gene="glmU"
                     /locus_tag="WIE35_001019"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ4153948.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMLGRVIDTAA
                     ALNPQNICVVIGHGKDQVLNAVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPSGLGRIIRDGSGSVTAIVEEKDADAA
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     AKLAADVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWMR
                     PEKDKQ"
     gene            8315..8644
                     /locus_tag="WIE35_001020"
     CDS             8315..8644
                     /locus_tag="WIE35_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ4153949.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVL
                     DANGAVLQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(8716..>8987)
                     /locus_tag="WIE35_001021"
     CDS             complement(8716..>8987)
                     /locus_tag="WIE35_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ4153950.1"
                     /translation="PRDPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLAD
                     GFIFVQNLLNGEPLEEYISPFGGGYFFVLPGVEKGGFLGQGLLGV"
BASE COUNT         2120 a         2700 c         2268 g         1899 t
ORIGIN      
        1 aagcttcagc taacctttga atattactgt tgttctaagg tctagattcc cgcctgcgcg
       61 ggaatgacgg gtcttttata acctttgaat attgccgtta tcccaaggtc tggattcccg
      121 cctgcgcggg aatgacgggt cttttataaa ctttgaatat tgccgttatc ccaaggtctg
      181 gattcccgcc tgcgcgggaa tgacggcatc ggtctgctgt tttcggacgg catttcggct
      241 caatccagca gtgcgtccac aaacgcgcgc gcgtcaaacg ggcgcaaatc gtctatgcct
      301 tcgcccacgc cgatatagcg gacggggacg gggcggtcgg aggcaagcgc ggcgaggatg
      361 ccgccttttg ccgtgccgtc tagcttggta acaattaaac cggtcagccc caatgcgtcg
      421 tcaaaggctt tgacttggtt gacggcgttt tgaccgatat tggcatcaag cacgacgatg
      481 atttcgtgtg gcgcgtcggg catggctttt tgcagcacgc gtttcacttt tttgatttct
      541 tccatcaagt gaagctgcgt gggcaggcgg ccggcggtgt cggccagcac aatgtcgatg
      601 ccgcgcgctt tggcggcttg gacggcatcg aagcacacgg cggcggaatc gcccgtggtt
      661 tgcgaaatca cggttacgtt gttgcgttcg ccccaagctt gaagctgctc acgcgcggcg
      721 gcgcggaaag tatcgccggc ggcaagcagc acagatttgc cctgcgcttg gaaatatttg
      781 gcgagtttgc cgatggatgt ggttttgccc gcgccgttga tgccagcaag catgatgaca
      841 aacggctctt tggtttcggg caaaaccagc ggtttctcta gaggcttaat caggtcgtac
      901 aaggcttctt tcagcgcgcc gcgcaattcg ttgccgtctt tcagcccttt gaggctgacg
      961 cggtcgcgca cgtctttcat caggtattcg gtggcttcca tgcccatatc gctggtaatc
     1021 agcacggttt ccagctcttc gtacaaatct tcgtcgattt gtccgccgcc gaacacgccc
     1081 gccagcgatt tcgccatttt gtcgcgcgat ttggtcaaac cttgtttcaa acgcgccgcc
     1141 caaccgagct tgtgttcttc agttgtcgca acggcttctt gaacttgccc gacagcctcg
     1201 ccgacggttt cggcaacggc ttcttttgcc gcttcgactt gttccgctgc tttttccgcc
     1261 gcttcctctg cttcagacag catctcggca acggtttcct ttacctgttc aaccgcaccg
     1321 ctgacggttt caacagccga ttcgacctgc cctttgacgc tttccgttaa agattcgact
     1381 tcttctttaa tattttcaac tatttgagta acttcggatt ctgcttttgc tgcagtttcc
     1441 tgaacttgag cctcctcggg agccggcgtt tcctgttttt tcttgcgacg gaagaagctg
     1501 aacattgaat tttcctttta attttagaaa cttgaaatag ggtgtattgt agcgtatttt
     1561 gcgcggcaag gttgtctgaa aatccgggct gtaaggtttc ggcatctcaa acgtctaatc
     1621 atgcaaaccg tccacacagg aaacattaaa atgaaacacc gtactttctt ttccctttgc
     1681 gccaagttcg gctgcctgct tgcgctgggt gcttgttcgc ccaaaatcgt cgatgccgga
     1741 gccgcgaccg tgccgcacac tttatccact ttgaaaaccg cagacaaccg ccccgccagt
     1801 gtttacttga aaaaagacaa accgacgctg attaaatttt gggcaagctg gtgtcctttg
     1861 tgtctgtccg aattgggaca gaccgaaaaa tgggcgcaag atgcaaaatt cagctccgcc
     1921 aacctgatta ccgtcgcgtc tccgggcttt ttgcacgaga aaaaagacgg cgacttccaa
     1981 aaatggtatg ccggtttgaa ttatcccaag ctgcccgtcg taaccgacaa cggcggcacg
     2041 atcgcccaaa gcctgaatat cagcgtttac ccttcgtggg cgttaatcgg taaagacggc
     2101 gacgtgcagc gcatcgtcaa aggcagcatc aacgaagcgc aggcgttggc gttaatccgc
     2161 gacccgaatg ccgatttggg cagtttgaaa cattcgttct acaaacccga cactcagaaa
     2221 aaggattcaa aaatcatgaa cacgcgcacc atttacctcg ccggcggctg cttctggggc
     2281 ttggaagcct atttccaacg catcgacggc gtggttgacg cggtatccgg ctacgccaac
     2341 ggcaacacga aaaatccgag ctatgaagac gtgtcctacc gccatacggg ccacgccgaa
     2401 accgttaaag tgacctacga tgccgacaaa ctcagcctag acgacatcct gcaatatttc
     2461 ttccgcgtcg ttgatccgac cagcctcaac aaacagggca acgacaccgg tacgcaatac
     2521 cgcagcggcg tgtactacac cgaccccgcc gaaaaagccg tcatcgccgc cgccctcaaa
     2581 cgcgagcagc aaaaatacca actgcccctc gttgttgaaa acgagccgct gaaaaacttc
     2641 tacgatgccg aggaatacca tcaggactac ttgattaaaa accccaacgg ctactgccac
     2701 atcgatatcc gcaaagccga cgaaccgctg ccgggcaaaa ccaagaccgc accgcaaggc
     2761 aaaggcttcg acgcggcaac gtataaaaaa ccgagtgacg ccgaactcaa acgcaccctg
     2821 accgaagagc aataccaagt tacccaaaac agcgcgaccg aatatgcctt cagccacgaa
     2881 tacgaccatt tgttcaaacc cggcatttat gtggacgttg tcagcggcga acctttgttc
     2941 agctccgccg acaaatatga ttccggctgc ggctggccga gcttcacgcg cccgattgat
     3001 gcaaaatccg ttaccgaaca cgatgatttc agctacaaca tgcgccgcac cgaagtgcgc
     3061 agccacgccg ccgattcgca tttgggacac gtcttccccg acggcccgcg cgacaaaggc
     3121 ggactgcgct actgcatcaa cggcgcgagc ttgaaattca tcccgctgga acaaatggac
     3181 gcggcaggct acggcgcgtt gaagagtaaa gtgaaataag ccgcaccgcc gcctaacccg
     3241 acaaaatacc gtctgaaacc tgcaacgttt cagacggtat tttttatccg gcggggattt
     3301 tgtccagacg gagattttgt ttagacggca tcgccgccgt tttcaatcag ccccgccaac
     3361 cgttccaacg cgaaggcgac cgcctgcgcg cggacggatt cgcggttgcc gtcaaaacgg
     3421 cgcattgctt cgcaacttcc gcccggaaag gcaaacccga accaaaccgt gccgacgggt
     3481 ttgctttcgc tgccgccgcc cggaccggcg atgccggaaa taccgacggc gtaatccgcc
     3541 tgcgccacgg ctttcgcgcc gcgcgccatc tcatagacgg tttggcggct gaccgcgccg
     3601 tgttcgagca gggtttcggg caacacgccc aagcggtctt ctttggcttt gttgctgtat
     3661 gttacaaaac tttgatcgaa ccattgcgaa ctgcctgcaa cgcttgtgaa tacggcggca
     3721 agcagcccac ccgtgcagga ttcggcacag cttacggttt gacgtttttt cgtcaggttt
     3781 cgggcgatgg tgtgcagcgc gtccatttcc cactcctctt tcagacggcg tttaagaatt
     3841 gatgatgtgt atgtcgcgtt gcgggaacgg gatgttgata ttgactttgc ggaggttttc
     3901 gaccacttgt tcgttcaagt cgcattgcag cgtccagcgg tctgcttcgt ttgcccaagc
     3961 ccataatgtg atttcgatgg cattgtcgcc caaggcggtg atgtaggcgg cggcctgccg
     4021 ctcttcgttt tgaacgctca aggggtgttc gacggcggct ttcaacaccg cctctttcgc
     4081 cactttcaaa tcgcagttgt aatcgacgcc gactatcact tgggcgcggc acagcggcag
     4141 tgtggaacgg ttgacgatgc tgttgcccat caccacgctg ttgggcagca cgacttcttc
     4201 gttgtcggtc gtccgcaaag aagtctgcac cattttaatc tctcggacat atccttcaaa
     4261 accgccgacg cggataaaat cgccgacttt gaacgggcgg aacaggataa tcagcgcgcc
     4321 ggcggcaaaa ttggacagct ggtctttcaa ggacaacgcc accgccaaac ccgcgccgcc
     4381 gattaaggcg gttacggatg ttgtggaaac gcccaatctg cccaatgcgg caataatcac
     4441 caaaatcaat aagccgatat tggcaacatt acacaaaaaa ctaatcagcg tggcatcgac
     4501 ctgcgcgcgc gtcatcgccg ccctcatcac agcgacaatg cgtttcgccg cccattttcc
     4561 gaccaaaaaa ataagcagcg cggcggcaag gttcagcccg aacgcccacg ccttttcagc
     4621 cagatgctcc caaccggaaa cactgatcag gtgtaaaaaa tcaaattgtt tgaagtccat
     4681 tgttttcctc ttgatcgaac atccgccccg tgcggcgtaa tcggcacagg tgtaaaaatg
     4741 ccgtatgaag ccctgcgggg cggtatgttt gttttatttc aaaaccgttc taatccaacg
     4801 ggaaacccat ccgcgctggt tcgcatcaat atcgcgctgc gcgggggcgg tggtgtgcgt
     4861 cggggcttgg gcgaagcgga acacgtcggg caggcgcgtg tttttgacgg cggggtaaac
     4921 ccacatttcg gacggaaccg cctgctgcac ttcccgactt tgcagccatt gcaccagttt
     4981 tgccgccaat tccggctgtt tcgcgccctt caagaccgcc gcgccttcga cctggcggaa
     5041 tacgccgcct tttaaaaaca ggttgcccgt cggcggctcg ctgtatttgc ctttggaaaa
     5101 atacacttcc gccgccgggc tggcggcata accgaccacc agcggatacg cgccgccgtt
     5161 gtgcgaaaag tcggtgtaat acgcctcgct ccagcctttg gcgaccttca cgccgttctg
     5221 ccgcatctgt gcccaccatt tgaacgcgcc ttcttcgccc agaccgctga tgttcgccat
     5281 caggaagccc agccccgggg acgacgtggc gggggacggc acgaccaata ggtttttata
     5341 ttcggggcgg gtcaaatcct gcagggtttg cggcaggggc agctttttgc cttcaaacca
     5401 ttttttgtcg taattgatgg acacatagcc gtaatcgacc gccaaagccg aaggcagccc
     5461 gaccgcgacg ggggcggatt cgggttgcgc cgccgccaaa atgcccattt cccgcgcctt
     5521 gccgatattg gcgttgtcca aaccgtacac cgcgtcggca atcgggttgg cgcggctcaa
     5581 aatcagtttg ttgagcattt cgttcgcgcc gcccgcctga ataatcgaca ccttcgcatc
     5641 gtttgcccgc tcgaagcgcg caatcagccc tttgggcagg ctgaacgact tatgcaccgc
     5701 cagcctgact tccgtctgcg cctgcaggta tgccgaaacc gccagcagcg gcagcagcca
     5761 aattttccgt ttcattccga gtcctcctct attcgctgta aaataacatt ctaacaaatt
     5821 ttcacggttc aaaatgcaag aaaacccgac cgtgtggctg ttcgacctcg acaacacgct
     5881 gcacaatgcc gatgccggcc tcttcacact catcaaccgc gctatgacac gctatatgac
     5941 acgccgcctc aaactctccg aatccgccgc gtccgacctg cgtcaagact attggcaccg
     6001 ctacggcgca acgctcgccg gactgcaaat ccaccatccc gaaatcgaca tcgccgaatt
     6061 tttgcgcgaa agccatccga ttgacgaggt tttgacccgt ctgcacggta tgccggaaac
     6121 acaaaacacc ctgagccgcc taaaagggcg caaggcggtt ttttccaacg gcccgtcgtt
     6181 ttacgtccgt gccgttgtcg gcgcactcgg tttggaaaac cgtttcgacg cgcttttcgg
     6241 cacggatgat ttcgggctgc tgtacaaacc caatccgcaa gcgtatctca atgtctgccg
     6301 cctgttggac gtaccgcccg aatgctgcat tatggtggac gacagcgcgg acaacctgca
     6361 tcaggcaaaa gcattgggca tgaaaaccgt ccggttcggt gcaaaagtgc acgacctgcc
     6421 ctttatcgat gcttccgtaa gcgatatggc gcaactggct cggtatgcag aaactttgtc
     6481 agaacaccgc caaaatcatt acaatacccg cacaaccccc cgaaaatacg aaagaatgtt
     6541 atgcgtaaaa ccttccttat cctgatgact gccgccgccc ttttgtcggg ctgcgcgtgg
     6601 gaaacttatc aagacggcaa cggcaagacc gccgtccgtc aaaaatatcc cgccggcacg
     6661 cccatttatt accaagacgg cagctactcg aaaaatatga actacaacca ataccgtccc
     6721 gaacgccatg ccgtgttacc caaccaaacc ggcaacaacg ccgacgaaga gcatcgccaa
     6781 cactggcaaa agcccaaatt tcaaaaccga taaacccacc ctatgccgtc tgaagccctt
     6841 tcagacggca ttgcacagga aaccgtcatg ccgcaaaaca ctttaaacat cgttatcctc
     6901 gccgccggca aaggcacgcg catgtattcc aaaatgccca aagtgctgca ccgcatcggc
     6961 ggcaagccca tgctcgggcg cgttatcgac accgccgccg cgctgaatcc gcaaaacatc
     7021 tgcgtcgtca tcgggcacgg caaagaccaa gttttaaacg ctgtcaaacg cgatgtcgtt
     7081 tgggttgaac aaaccgaaca gctcggtacc ggccacgccg tcaaaaccgc cctgccccac
     7141 cttgccgccg aaggccgcac gctggtgttg tacggcgacg ttcctttaat tgacgttgaa
     7201 accctcgaaa ccctgctcga agccgcaggc aacgaagtcg ggctgttgac cgacgtgccc
     7261 gccgacccgt caggtttggg gcgcatcatc cgcgacggca gcggcagcgt aaccgccatt
     7321 gtcgaagaaa aagacgccga cgccgcccaa aaagccgtga aagaaatcaa taccggcatt
     7381 ttggttctgc ccaacgccaa actggaaaac tggctgaaca gcctctccag caacaatgct
     7441 caaggcgaat attatttaac cgacctcatt gccaaagccg ttgccgacgg tattaaagtt
     7501 catcccgtcc aagtccgcgc ctcccacctc gccgccggcg tgaacaacaa acgccagctc
     7561 gccgaactcg aacgcatctt ccaaaccgaa caggcgcaag aattgctcaa agcaggcgta
     7621 accctgcgcg atccggcacg tttcgattta cgaggccgtc tgaaacacgg acaggatgtc
     7681 gtaattgatg tgaactgtat ctttgaaggc gaagtcgaac tcggcgacaa cgtcgaaatc
     7741 ggcgcaaact gcgtcatcaa aaacgccaaa atcggcgcaa acagcaaaat cgcccccttc
     7801 tcccacctcg aaagctgcga agtcggcgaa aacaaccgaa tcggccccta tgcccgcctg
     7861 cgtccgcaag ccaaactagc cgccgacgta cacgtcggca acttcgtcga aatcaaaaac
     7921 gccgccatcg gcaaaggcac caaagccaac cacctcacct acattggtga tgccgaagtc
     7981 ggcagcaaaa ccaacttcgg cgcaggtacg attattgcca actacgacgg tgtgcacaaa
     8041 cacaaaaccg ttatcggcga cgaagtccgc atcggttcaa actgcgtcct agtcgccccc
     8101 gttaccctcg gcaacaaagt aaccacaggc gcaggcagca cgattacccg caatatcgaa
     8161 gacaacaaac tcgccctcgc ccgcgcccgc caaaccgtca tcgaaggctg gatgcggcct
     8221 gaaaaagaca agcaataaag ctatgccgtc tgaagccggt ttcaggtttc agacggcatc
     8281 aaactcctcc gatatcagat aaggactgca aaccatgtca ttacccccat gcccacaatg
     8341 cgcctccgaa tacacttatg aagacggcgg acaatacatc tgccccgaat gcgcccacga
     8401 atggaatgaa accgaatccg ccgccgacct tgcggctcaa gtgctcgatg ccaacggcgc
     8461 agtgctgcaa aacggcgata ccgtcatcct catcaaagac ctcaaggtaa aaggcagctc
     8521 gatggtgatc aaacaaggca caaaagtcaa aggcatacgc ctgcaagaag gcgatcacaa
     8581 catcggctgc aaaatcgacg gcagcgcgat gaatttaaaa tccgaattcg tcaaaaaagc
     8641 ctgaccgccc aaaaacaaga aacgccgtcc gaacccgttt ggcaaggttc ggacggcgtt
     8701 ttttatatgg cggatttata cgcccagcag cccttgcccc aaaaagccgc ctttttccac
     8761 gccgggcaag acgaagaaat agccgccgcc gaaggggctg atgtattctt ccagcggttc
     8821 gccgttgagg aggttttgca cgaagatgaa tccgtcggca aggtttgcct gatagcagac
     8881 gaacaccagc ccgacatcaa gctgtccgct tgaggcgagt ccgcgcgaat agctgtaggc
     8941 gcggcggaag aggcggtgtt ttttgaggaa ttcgggatcg cgcggat
//
LOCUS       DAWHXZ010000069         8923 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000069 DAWHXZ010000000
VERSION     DAWHXZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8923
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.069"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(25..1626)
                     /locus_tag="WIE35_001022"
     CDS             complement(25..1626)
                     /locus_tag="WIE35_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ4153951.1"
                     /translation="MTEQHFTEQTKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKQIRKENPDEPIEFINMDIEGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAAHNAELFSVKTEGGAKIVLFERISQYITDEGRRDDFCHALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPKEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHAIGEDKCMIYTQDISQKSSNLLRLNLILNNLVHSLNNVVQG
                     NTILSPAHKDTSGRLKKFDFIVSNPPFKLDFSDFREQLEGEENRERFFAGIPKIKAKD
                     TDKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAQSSIDKKIREYLVENKMLAGMVS
                     MPSNIFATTGTNVSILFIDKANKDNVVLIDASGLGEKIKDGKNQKTVLSREEEQKICN
                     TFTHKQAVEDFSVVVGYDEIKAKNYSLSAGQYFEVKIDYVDISADEFAQKMAGFSADL
                     DKLFAESAKLEKEIKDRLAMLEFNS"
     gene            complement(1674..2867)
                     /locus_tag="WIE35_001023"
     CDS             complement(1674..2867)
                     /locus_tag="WIE35_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ4153952.1"
                     /translation="MKRQITIGEIAEINANSLTQKDMFQEIMYLDTGNITRNEIDNIQ
                     VLNVMMDKIPSRAKRKVKDKTIIYSTVRPNQEHYGFLENPGDNFIISTGFSTIDVYDE
                     NTDEKFIYYLLTQKHITDYLHTIGENSVSSYPSINPDDIANLKFAVPDLKTQQSIAAV
                     LSALDKKIALNKQINARLEDMAKTLYDYWFVQFDFPDANGKPYKSSGGEIVFDETLKR
                     EIPKGWGSIELQSCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNP
                     QDAHIIFGDHTRIVKLVNFQYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYA
                     RHFKFLKEFKIILPSKDISQKYNEIANTFFVKVRNNIKQNHQLTQLRDFLLPMLMNGQ
                     VSVAE"
     gene            complement(2994..5999)
                     /locus_tag="WIE35_001024"
     CDS             complement(2994..5999)
                     /locus_tag="WIE35_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ4153953.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNENWDKPTNIFPKI
                     FIESLCRINPDLSPDDARRLLTDIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEEFRPDITLLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     SKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSAYGKPVFNYFREEHKLNLAEL
                     LNTLSDGLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLSFLLQHGL
                     TYVKASQGLVQKHIMRYPQLFATLAIEKHLANGGKKGVIWHTQGSGKTALAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLVVHTIDSREAFAADIKSAQTLHN
                     HAGKAEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREAISSQYK
                     AQLQAALAQLEVEKGSFDRKEIYAHPHFVRPMLDYILGDFAKFRKTNQDESLGAMVVC
                     DSAEQARQLFEHFQTASDHNFTAALILHDVGTKDERDQWVKDFKAGKTDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAY
                     WDELSNELGDEIGSYSQLFKTAEEIEQEIADIKNALFDFDTENAEEFCSQISQIEDKK
                     QLLALKKALQTSKELYNALRLQGSHEFLVHLDFDKLNLLYRETAARLDTLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQDDPEYISLR
                     EELERIFKKKNLAEVGQEEMQANIATLQIVYAKIKELNRQNDLLRHKYGGDAKYARTH
                     KRLMENAAFYGDKLKVFNALNGVKADADQKVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPQDIQAINRLLVREYLKESGRM"
     gene            complement(6113..7423)
                     /locus_tag="WIE35_001025"
     CDS             complement(6113..7423)
                     /locus_tag="WIE35_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ4153954.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            7735..8148
                     /locus_tag="WIE35_001026"
     CDS             7735..8148
                     /locus_tag="WIE35_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153955.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNLQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASEKNTDKQ"
     gene            8242..8923
                     /locus_tag="WIE35_001027"
                     /pseudo
     CDS             8242..8923
                     /locus_tag="WIE35_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244588.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT         2229 a         1941 c         2143 g         2610 t
ORIGIN      
        1 ttgaccggtt tcattcatcg gtatttacga attgaattcc aacattgcca gcctatcctt
       61 aatctccttt tccaatttgg cagattcggc gaaaagttta tccaaatccg ctgaaaatcc
      121 cgccattttt tgcgcaaatt cgtcggcgga aatatccaca taatcaatct ttacctcgaa
      181 atactgcccc gccgacaggc tgtaattctt ggctttgatt tcatcgtagc cgaccaccac
      241 gctgaaatct tccactgcct gtttgtgcgt gaaagtattg caaatttttt gttcttcttc
      301 gcgggagagt acggtttttt ggtttttgcc gtctttgatt ttttcgccca agcccgatgc
      361 gtcgattaat accacgttgt ctttattggc tttatcgata aacaggatgg aaacgttggt
      421 tcccgtggtg gcgaaaatat tggaaggcat ggaaaccatg ccggcgagca ttttgttttc
      481 taccagatat tcgcggattt ttttgtcgat actcgattgg gcggtgataa agcccgtcgg
      541 caacacaatc gccgctttgc cgttttcttt caggctaaac aagatatgct ggataaaaag
      601 ctggtaaatt tccatcttat ccgtatcttt tgccttgatt tttgggatac cggcaaaaaa
      661 gcgttcgcgg ttttcctcgc cttccaattg ctcgcgaaaa tcgctgaaat ccaatttaaa
      721 cggtggattg gaaacaatga aatcaaattt tttcagacga cctgaagtgt ctttatgggc
      781 gggcgacaaa atcgtattac cctgaaccac attattcaaa gaatgcacca aattgttcaa
      841 aatcagattc agccgcaaaa gattggacga tttttgcgaa atatcctgcg tataaatcat
      901 acatttgtct tcaccgatgg cgtgcgccac gttcatcagc agcgttcctg aaccggctga
      961 cggatcgtaa acgtccacgc tgcggatttg cccacgtacc tcctttggca ccaaaatatc
     1021 cgccataatc cgcgctactg cgtgcggcgt gtagtattcg gcatatttgc cgcccgaatt
     1081 actgttgtag tctttaatca ggtactcaaa aatcgtggcg aaaaaatcaa atttttgcgc
     1141 aaaaatcgcc tcgaagctga aacccgccag tttggaaatc agcgcgtggc aaaaatcgtc
     1201 gcggcgacct tcatccgtaa tatattgact gatacgctcg aacaacacga ttttcgcccc
     1261 gccttcggtt tttaccgaaa acagttccgc attatgtgcg gcaatatcgg tcagcgtatc
     1321 gtcaaagagt ttggcaaagt ccgcgccgtt ttgtcgctct gataaatact tgatggaatg
     1381 ttcaggcttc aaaacggctg tttttccctc aatatccata ttgatgaatt cgattggctc
     1441 gtcgggattt tctttgcgaa tttgtttaac ttcgaaatcg tatttgtcgt ttaggaattt
     1501 atacaaaaat gcctgactga tgattttgaa ctcgttgccg tcattgccca aaccatagtt
     1561 ggcgcaaatg gtttttaagc tgtcgattag ggatttggtt tgttcggtaa agtgttgttc
     1621 ggtcattttt ttctcgtttg ttttattttg taggtctgat tcttgaattt ggcttattcc
     1681 gccacagaaa cctgcccgtt catcagcatg ggaagtagga aatcgcggag ttgggttagt
     1741 tggtggtttt gttttatatt atttcgaact ttaacaaaga aagtatttgc tatttcatta
     1801 tatttttgag aaatatcttt actaggtaaa attattttaa attcttttaa gaatttgaaa
     1861 tgtctagcat atccataact tgataaatct atttgattta tgatttggta aaacaaatag
     1921 tttggcattc gttcattatt gcttaatatt acttgtgtac catctgctcc ccgcgcatat
     1981 tgaaaattaa ctaacttaac aattcttgta tgatcaccaa aaataatatg agcatcttgc
     2041 gggtttaata tagatttttc atcattggta aatccacaaa taaaatcttg actttggtca
     2101 acaacaggat attttccaaa atccttaata tctttattta atatttttgt agtatttggg
     2161 atttttgcta agcaagattg taattctata cttccccacc ccttcggaat ttcccgtttc
     2221 aaggtttcat caaacacaat ttcgccgcct gaggatttgt agggcttgcc gttggcgtca
     2281 gggaagtcga actgcacgaa ccaatagtcg tacagggttt tcgccatatc ttccaggcgc
     2341 gcattgattt gtttattcag ggcgattttt ttgtccaaag cggataggac ggcggcgatg
     2401 gattgttggg ttttgaggtc aggaactgca aattttagat ttgcaatatc atcaggattt
     2461 atcgatggat atgatgaaac actgttctcg ccaattgtat gtaaataatc agtgatatgc
     2521 ttttgagtca ataaatagta aataaatttt tcatccgtat tctcatcata aacatcaatt
     2581 gtactaaacc ctgttgatat aatgaaatta tcaccaggat tttccaaaaa accgtaatgc
     2641 tcctgatttg ggcgaactgt agaatagata atagtcttat cttttacttt tctttttgca
     2701 cggctaggta ttttatccat catgacattc aaaacttgga tattatctat ttcatttcta
     2761 gtaatattgc cagtatccaa atacatgatt tcttgaaaca tgtctttttg agtgagggag
     2821 ttggcattta tttctgcgat ttcaccaatt gtaatttgtc gtttcatctt aacttaatct
     2881 ctcgaaactc ttcaaaggcc gtctgaaaaa aatttcaaaa tccaaccgca cttttattgt
     2941 ccacttcgaa cgtatgcaat acgcccataa aggctaccta aaaaaccgct ttctcacatc
     3001 cgcccgcttt cttttaaata ttcccgcacc aacaggcggt tgatggcttg gatgtcttgg
     3061 ggttgaacag gaaattgttg ctccgttcta aagcgtttta atacgatgcc ctgcatttgt
     3121 ttttcaaagt aattttgatt gtccaaaatc tgctccatat ccagcacttt ttggtcggca
     3181 tcggctttga cgccgtttag agcgttaaaa actttgagtt tgtcgccgta gaaggcggcg
     3241 ttttccatca gccgcttgtg ggtgcgggca tacttagcgt cgccgccgta tttgtgccgc
     3301 aaaaggtcgt tttggcggtt cagctctttg attttggcat agacgatttg gagtgtggcg
     3361 atgtttgcct gcatttcttc ttgcccgact tcggcgaggt tctttttctt gaagatgcgt
     3421 tccagctctt cgcgcaggct gatgtattcg gggtcgtctt ggtcgaagtt gcccgccagc
     3481 ccttcacgga ctttgcgcat gatgtctttt aaatcgtcgg caagtttgag ttccgcctcg
     3541 ccgattttga cgaattgaaa gtaaacgtct tccagcgctt cattgagcaa atgggcggtg
     3601 tcgccttgct gtaatttttc ggcaaggttt aaggtatcca gacgggcggc agtttcgcgg
     3661 tacagaaggt tgagcttgtc aaaatccaga tgcacaagaa attcgtggct gccttgcagg
     3721 cgcagagcgt tgtatagctc tttggaagtt tgcagggctt tttttagggc gagaagctgt
     3781 tttttgtctt ctatttggct aatttggctg caaaattctt cggcgttttc ggtatcgaaa
     3841 tcaaacaggg cgtttttaat gtctgcaatt tcctgttcga tttcttcggc ggttttgaat
     3901 agctggctgt aactgccgat ttcgtcgccc aattcgttag aaagttcgtc ccaataggcg
     3961 cggttggttt tgtcaaattc gcgttcgatg tcggcaaaat cgacaacata gccgtaacgg
     4021 taagatttgt aggtgcggtt gacgcgggtc agggtttgta aaaggttatg ggctttgatg
     4081 agtctgccca aatagagctt tttcaggcgc ggcgcgtcaa agccggtcaa aagcatgttg
     4141 tacacaaaca ggatgtcggt tttgcctgct ttgaaatctt tgacccattg gtcgcgttcg
     4201 tctttggtgc cgacgtcgtg caatatcagt gcggcggtga aattatggtc tgaggccgtc
     4261 tgaaaatgct caaaaagctg gcgcgcctgt tctgcgctat cgcaaacgac catggcgccg
     4321 aggctctcgt cttggttggt ttttctgaat ttggcgaagt cgcccaagat gtaatcgagc
     4381 atcgggcgga cgaaatgcgg atgggcgtag atttctttgc ggtcaaagct gcctttttcg
     4441 acttcaagct gcgccagtgc agcctgcaat tgggctttgt attggctgct gatggcttcg
     4501 cgaatcaggc gcagggtgta gccgtcggcg atggaggcgt tgtaatagta tttatggatg
     4561 tagtcgccga agagttcgcg ggtattgacg ttgcctgcgg ttacgccgat aagcggcgtg
     4621 ccagtcagcc cgattttgac ggcgtttacg tcggactggt tgaggttggc aagaaacgag
     4681 cctttggggt tgtagctgcg gtgtacttcg tcgagaaaat agacgcgctg aatggcgagg
     4741 tcgtagtcgt tacgagcgac gacatcgggg tcgtcttgga atttttggat attaacaaca
     4801 gtgatttccg ctttgcctgc gtggttgtgc aaagtttggg cggatttgat gtcggcagca
     4861 aaggcttcgc ggctgtcgat ggtatggacg actaaatcgc gggcagtaaa ttcgcgctgc
     4921 gcctgtttca ataaatcaag cctgtccacg atgaaataga atttcggcac gatgccctgt
     4981 ttggcataat agtgggtcag gtagcgggtg ttgtaatagg caagcgcggt tttgccagag
     5041 ccttgggtgt gccaaatcac gccttttttg ccgccgtttg ccaaatgttt ttcaatggcg
     5101 agagtggcaa atagctgcgg ataccgcatg atgtgttttt gcaccaagcc ttgacttgct
     5161 ttgacgtaag tcagcccgtg ttgcagaaga aaagaaagac gttcgcggca aagcagcgaa
     5221 gtcaggatgc ggttggtcgg cgtatcgggc gatttattgc tgataaattc ggggctgtgt
     5281 ttgataacgg gcaggttgtt gtcttgcagg acgttgtttt cgaggccgtc tgaaagcgtg
     5341 ttcaataatt cggcaagatt taatttatgt tcttcgcgga agtagttaaa cacgggctta
     5401 ccgtaagcag acgaagcgta aaacgcgcct tgtgccggtt cggttgcgcc gtcgtcgtat
     5461 tccatgttgt tggaaaaaat catgaattgg gtgatgttga tgaaacggcg aaatttggaa
     5521 tttttcgcac gtttgcccat gcgctcccgc tcttcgccga ttccgccttt gttgttgggc
     5581 tttttgactt cgataaaaac caaaggcatg ccgtttacca gcagggtaat gtcggggcgg
     5641 aattcttcat cgccgttgat acagggcaat tcggttacaa cgtggaagct gttgttatca
     5701 aaattttgga agtcgatcag tttcttgccg ccggattgat tggtaaggcg ttcgtaaaac
     5761 ttttgcccca agtcttcgtt gtccagttct aagcgtatgt cggtcagcaa acggcgtgca
     5821 tcgtccggcg acaaatcggg attgatgcgg cagaggcttt ctataaagat tttgggaaag
     5881 atgttggttg gtttatccca attttcgttt ttgagcgaga ggtagtcgta gcccaagcgc
     5941 atcaggtgca aaacggcggg gattttgacg cgggaatttt cgttgtgagt ttgggacata
     6001 aacgtaatga ttcatcaaaa tttattgaaa cggattataa cacctgaaaa acccgatacg
     6061 cctttttggt gtaccggctt tgccataccg ttctgcttca gacggcattg cttcattttg
     6121 cctttaatgc ctcttcatcc agcgatttga gccacgccag cttttcgccg attttgattt
     6181 ccaacccgcg cgggacgggt tggtagaagt ccggttcgtc caagccgtcg ggcatatagc
     6241 tttcgccggc ggcgtaggcg ttcggttcgt cgtgggcgta gcggtattcg cgtccgtagc
     6301 ccaattcctt catcagcttg gtcggggcgt tgcgcaggtg gacgggcact tcgtcgctag
     6361 cgttttcttt gacgaagtgg cgcatttggt tgtatgcctt gtagcccgcg ttggatttcg
     6421 cggcggcggc aagatacaat accgcttgcg ccaaagccag ttcgccttcg ggcgagccta
     6481 agcgttcgaa ggtggcggcg gcatcgttgg cgatttggaa ggcgcgcggg tcggcaagcc
     6541 cgatgtcttc ccaagcgata cgcacgatgc ggcgggcgag gtagcgcggg tcggtgccgc
     6601 cgtcgagcat acggcagaac caatacagcg cggcgttcgg atgcgaaccg cgcacggatt
     6661 tgtgcagggc ggagatttgg ttgtagaaac tctcgccgcc tttgtcgaaa cggcggattt
     6721 gcgccccgag actgtcggcg agaaattcgg cggttaagtt tttcagacga cgtgtatcgg
     6781 cggcgcgtaa aagttgttcc aacaaattca acaatctgcg cgcatcaccg tcggcggtat
     6841 tcacgagtaa tttttgcgca tccgtttcaa tcgtaaactc ttggtattca ggcaaagcca
     6901 ataccttggc aatcagcttt ttcaggtcgt ctgaagacaa gggttgcaaa acatacacct
     6961 gagcgcggct caacagcgcg ggattgactt caaacgacgg attttccgtc gtcgcaccaa
     7021 taaaggttag caaaccgctt tcgacatgcg gcaaaaacgc gtcctgctgc gccttgttga
     7081 agcggtggac ttcatcgaca aacaaaatcg tcgcgcgtcc ctgctgcaaa gcgatttcgg
     7141 ctttatcgat tgcctcgcgg atgtccttca cgccggaaaa tacggcggaa acaggcaaaa
     7201 actgggcgtt gaaactctgc gccaaaatcc gcgccaacgt cgtcttgccc acgcccggcg
     7261 gcccccacag caacatagaa tgcggcttgc cgccttctac cgccacgcgc aaaggtttac
     7321 cttcgccgat gaggtgttcc tgccccacca cgtcgtcaag cgtatgcgga cgcaatcgtt
     7381 cggcaagcgg cgcgtcgggt tctcgggcaa acaaatcggt cataacggct ccgtcaacag
     7441 gttttcaaac aatatgatta tacggcaggg aacggcggcg tgccgcatac ggattccgcc
     7501 cctccgtttg ccttaagccg atattaggcg catactggaa aagacgagag acttcacaca
     7561 atatatccgg cacggagacc gattccgcat cggcatgaca atacccaaat cagcgtttca
     7621 attaaacatt aaggagacta aaatagaaaa tttgctttat ctaccattgc tttgttgatt
     7681 caatcggcat tatgttttga ggcggaagcc catgaatata ctaatattca agagatggaa
     7741 tgggtgtctt tattttctga tccgcaaaga gacgatgata gtcttataac ccttaaagat
     7801 gaaaaaatca ctgtaaaaaa ctatattgtg ccttggtgga aaaaaggtga aaactttaga
     7861 aaattagaac ttattaccac tggtaaaaat gataatggaa atttaacggt aaatggaatt
     7921 tacggcatcc aatcttttgc agactctcaa ttgggatata cgcctgaatt attggtaaaa
     7981 agcaataatg atattctatt taaaaataat ctccaatctg ttgacgtgaa gccccattcc
     8041 ggaaatatcc atttggatgc caaaggaaaa attcaattta atttgaatgt tcccgatgtt
     8101 gatggggtac aagatgtagc cagtgaaaaa aataccgaca aacaatagtg ccttagttcg
     8161 gtatgtcaac actatcagcc ccccgatgaa aatgacaaag gcagcatatc ctttcattca
     8221 aaaacaggca atttgttcaa tatgaacgtt aaagttcata aagacacaca ccatttcggt
     8281 attcacaacc tcaacaatac cgatgttcat ctgactgccg aagacagcga taatgtgatt
     8341 gagattaaag gggaactcgc cgaccctatg ccgacaggaa atgttgagag tacaagtttt
     8401 ttaaggggca ttgaaacggc ttttaaatcc aataccgtat taacggggaa taacaataaa
     8461 atatccatcg ccactgaggg caagggcttg ggattgggcg taaatggtct ccagtccaac
     8521 tccccctatt ccggacacac gacgggcatt ttcatcaata atcaatcaag cgaccttacc
     8581 caaccttccc ttgatggcat cagcaccatc aaactcaaag ccgtcaaggg aaataatgaa
     8641 gttgatttaa atgtaaaaaa ccacgcctcc gtaaaaggca ttatcgcgtc gcattctgca
     8701 aaagcaacgc ttgaagccga aggggacaat attgtccagg ttaaaaatcc ggacaccaat
     8761 acggctttaa tcgatgcttt gaaaaaacaa taccccaatg cttcgaaaga actatacagg
     8821 gtgggggttc aatccacgac tggaacagta cgcatttcgc caaacggcaa aatcgcatta
     8881 acggaattga aaacttttgg aattgtacaa aacggcattt gcg
//
LOCUS       DAWHXZ010000070         8875 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000070 DAWHXZ010000000
VERSION     DAWHXZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8875
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.070"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(64..1206)
                     /locus_tag="WIE35_001028"
     CDS             complement(64..1206)
                     /locus_tag="WIE35_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ4153956.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIATGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDSDRRIVQTALDNARSA
                     EVDDIVSFGVADAQSVRPNGENGIMVSNPPYGVRLEEVHALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1255..1677)
                     /locus_tag="WIE35_001029"
     CDS             complement(1255..1677)
                     /locus_tag="WIE35_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153957.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1670..2479)
                     /locus_tag="WIE35_001030"
     CDS             complement(1670..2479)
                     /locus_tag="WIE35_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ4153958.1"
                     /translation="MIQDTRPLIRVVAGILLNRDGDYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALRREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMVVQTREQWRQAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2561..2938)
                     /locus_tag="WIE35_001031"
     CDS             complement(2561..2938)
                     /locus_tag="WIE35_001031"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ4153959.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAVRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3138..3905)
                     /locus_tag="WIE35_001032"
     CDS             complement(3138..3905)
                     /locus_tag="WIE35_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ4153960.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMILFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIAKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3915..4694)
                     /locus_tag="WIE35_001033"
     CDS             complement(3915..4694)
                     /locus_tag="WIE35_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ4153961.1"
                     /translation="MNQKYILSANNNSLIEEIHNTVQRIGYCIVRGLNLNHLDDSRRN
                     KKLFDFLSQLGMLTNHKDDGFKSIFWDIKYRGDDYVINNDITFSEDVGECPLHSDSSF
                     SENPESYLVMYVVKSANDGGNSLFLSSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPA
                     SFDKKQGVRWGNILSVNTQMIRFRSDCIYKGIEENRNKVSKEMVLALDYLINVIKNAS
                     DIQEFSAQDDGLIIIDNVNGLHARTDYTDKNRHYIRARITV"
     gene            complement(4741..5469)
                     /gene="pdxJ"
                     /locus_tag="WIE35_001034"
     CDS             complement(4741..5469)
                     /gene="pdxJ"
                     /locus_tag="WIE35_001034"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ4153962.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQDKIAEFTKILTDAGIRVSLFIDADDRQIQAARDVGAPVVELHTGAYADAR
                     SHAEQIRQFERIQNGAHFASDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLISQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(5493..6284)
                     /gene="recO"
                     /locus_tag="WIE35_001035"
     CDS             complement(5493..6284)
                     /gene="recO"
                     /locus_tag="WIE35_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ4153963.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWIGGWPQPQGRALFSGLYVNELMLK
                     LTVREDPLPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLNRDGDGGTI
                     AAGGTYLVRPETAVFPVGKGFAVPPHAAGVVAPGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(6318..7445)
                     /gene="pheA"
                     /locus_tag="WIE35_001036"
     CDS             complement(6318..7445)
                     /gene="pheA"
                     /locus_tag="WIE35_001036"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ4153964.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(7484..8716)
                     /locus_tag="WIE35_001037"
     CDS             complement(7484..8716)
                     /locus_tag="WIE35_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ4153965.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLIVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQHFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHNGSATVMAVTMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
BASE COUNT         2085 a         2391 c         2320 g         2079 t
ORIGIN      
        1 atcggccgga tattaaaaaa agccgtctga acatttttca gacggcattt ttgtacactt
       61 tcctcaacgg ttcgatccct gcaccatatc aatcaggaac aggcggcaat cgaggttgcc
      121 gttataaagc ggaattttgc gttttggcga caggcgcatg aatttgggca tttccctgtc
      181 gccggtaaac atgccgaccc gccagccggc atagtgtttt ttcaaccacg tacccaactg
      241 cggataaagc gcctgcaaag cgtggacttc ctcaaggcgc acgccgtagg gcggattgga
      301 caccataatg ccgttttcgc cgttcggtcg gacggactgc gcgtcggcaa cgccgaagga
      361 aacgatgtcg tccacttcgg cactgcgtgc gttgtccaat gccgtctgaa cgatgcggcg
      421 gtcgctgtcg ctgcctgcaa tcggggcgcg gacggggcgg gtttgcgctt cggcgcggcg
      481 gcgcaaatcc gcccacagcg ttttatcgaa attttgcagt ttttcaaaac cgaagcggcg
      541 catcatgccc ggtgcgcggc ctgtggcaat ccaagcggct tcgatagcaa tcgtgccgct
      601 gccgcaaaac gggtcttgaa acggctgcgt gccgtcgtag cctgccgaga gcagcagtcc
      661 ggcggcaagg ttttcgcgca gcggggcttc gccggtatcg agacggtagc cgcgtttgaa
      721 cagggcttcg cccgaagtgt cgatgaagat ttcgacattg cgttcgtcca aaaaggcgtg
      781 gatgcgaaca tcgggcgcgg ctttgtccac gctcggacgt gcgtcgtaaa tgtcgcggaa
      841 agcgtcgcag acggcatctt tgacggtcag tccgacaaat tggatgctct taacgttggc
      901 acgctttgcc tcgactttga ctttgaacgt ctgctgtaaa gtaaaccaat taaaccagtt
      961 gatatttttg gcgagtttgt agatgtcgcg ctcattgcgg tatgtccctt tggtcaggcg
     1021 cagcaggata cggctggcag tacgcgaatg caggttggcg gcgtaaacct gttccaatcc
     1081 gccccggcag gaaacgccgc cgtcaaacac ttgtacatcg gtacagccga ggctttcgag
     1141 ttcttgagat aaaacggtct ccaagccgcg cgggcaggtg gcgaaaagtg tatataaagt
     1201 attcataacg tgtcctttcc ggacggatgt tccggcaatg gcggattgaa aggtttattc
     1261 ggcggtaaat gccgtctgaa atgtccgact gccgattgta atgtccgccg taaaatcgcg
     1321 cctgccttcg acacagatgg gcaggcggat gcgtactgcc tgccaagtcc ccgacggttg
     1381 ccgctcgaac atatagcggt tgaaacccat atccatattt ttcatactga agctgatgct
     1441 gacctgttcc gtgccggcgg gcgcgtgttc gatataaata tcaaacggtt ttttggttga
     1501 aacggcggcg gcgcggacgc ggcttccgtc cggcagcgtg caaccctcgg tcaaatcgca
     1561 ttgcgccgcc acagcttgcg gctgctgcgc ctgccaccat tgcaacaaaa cgagcttgac
     1621 ggcggcaaag gcaatcagca gcagtgcggc aagcagtaat ttacggtttt tattcatgat
     1681 tcttccctat tgtttcatcc cttaccacca cgtgcgcccc caatccgagc catagttttc
     1741 cataacgtgc aaccgtctgt ccgtttgctg caagtacgag cggcacggat acgccctgcc
     1801 gcagggcttc tgccgcctct tgtgcctgaa catcatcgca cacgcgccaa acgaccgcat
     1861 ccgcgccctt ttcctgcgcc tgccgccatt gttcgcgggt ctgcaccacc atccacacgc
     1921 tgtcggcgtg ttcgggtctg tcctgccatt gcgccgcctc catcaaaacg ttcgcaccgc
     1981 ttccctctgc cgaacccaaa ggcaggacgc ggtacgcgcc catactgttc tccccgtgca
     2041 aacccgtttt caggctgccg tacaaacggc gcggaacgga cagcgaacgc aaaagcgcgc
     2101 cgttggcggg cagcatgggg gcaacggtaa aatcacccgc cttctgccaa gaccattcct
     2161 gcccttcgcg ggattgcggt tcgcccgtcc attggccggg gttgacccat aggaatttca
     2221 ggcagacgcg ggcgtgttcg taggaatgga ttttggtcag ccaaggcgtg gcggcgagga
     2281 tgcggatgcc gagttcttct tcaaactcgc gccgcagggc ttggaagtcg gtttcgcccg
     2341 cttcgacctt gccgccggca aattcccaat atccggcata gggtttgcct tcggggcggg
     2401 aactgagcag gtagtcgccg tctcggttga gcaggatgcc ggcaacgacg cggataaggg
     2461 gtcgggtgtc ttgaatcatg gcgggtacgc ggttggtcgg tcggagtgca aatgagggac
     2521 gggcaatgcc gtcaacgggt ttgccgcatt tcagacggca ttatttttcg gcaacgacaa
     2581 acgccaatac ggtgtcttct tcgtcgctca tgctgaggct gacgcggctg atgccttgtt
     2641 cctccagcca tttggacagg gcggggccgt agaaaaattc gggcttgccc aatgcgtcat
     2701 gcccgatgcc gatgttgcgg aaggaaaccg cgccgcgtat gcccgtgccg acggctttgg
     2761 caaaggcttc tttggcggca aagcgtttgg caaggtagtt gacgggtttg cccgcctgag
     2821 gaaattcaag cagctcttcc ggagtgagga tacgcacggc aaacgcctgt ccgaattttt
     2881 tgtttaagcg gatgatgcgc ttgagggaaa cgatgtctgt accgatgccg taaatcatat
     2941 ttgcgctcct tcgcccttgg ttgcgggtag tgatgaatgt gatgatgaat gccgtcaatt
     3001 tcttggggcg ttaaaatggc gtgaatatcc actttcgggg ttttgtcggt aacaattttt
     3061 attgtaacct tttgcatttg aaattctaaa acatcgtgcc aattatttaa taaggagtta
     3121 aggcatttaa taatgaatta aagtttgatt aaacccgaat gcccgatttt cagagccaat
     3181 accaataaaa caataaaaat taacatttta aaaaaatttg ggctaatctt agtccttaac
     3241 cgaattccaa catacaatcc aataacagac aatacggaca gtaaaaatat taaaccgtat
     3301 tcactcttat ttaataacca atactggtct cttagcatat atatttgaac aattttcgcc
     3361 aaaagatagc atagattgct tgattttgcg atacgatttt tattctctgt ttcgctaagc
     3421 aaaaatatta acaatatggg agacatggca ttggttgaac cgccgatgat gcctgccaaa
     3481 aacccaaaaa gaatcatatt cttattattg gcaactactt gaatattttt tgcttttgca
     3541 catacattta aaataccatt aacagaataa tacaatgtaa tgattgccat cagtaaaagc
     3601 agccacgaca ctggaagtat caaaagcaac ttcaccccca aaatgctgcc aacgacgctg
     3661 ccgatagcaa gcaatttata ggtttttaaa taataaacaa tctcttgcca aaaacccttt
     3721 ttgttattgc tgcatagaac caacaagctc attaacaggc ttggtaatgc caccaaggca
     3781 acaaccttag acaatggcat gataaaagcc aatgcggttg taccgagcat cggaaatccc
     3841 atgcctgtaa ttccgtgcag tattgcggca gcaacaaaaa cgatagattg cattatttct
     3901 tgcataaccg tcctttatac agtaattctt gctctaatat aatgcctgtt tttatccgta
     3961 taatcagttc tggcatgcaa gccattgaca ttgtcaataa taatcaaacc atcatcttgt
     4021 gcagaaaatt cttgaatatc actcgcattt tttataacat ttataagata atcaagtgca
     4081 agtaccattt cctttgatac tttatttcta ttttcttcaa tacctttata gatacaatca
     4141 cttctaaatc taatcatttg agtattgacc gataagatat taccccatct cacaccttgt
     4201 tttttatcaa atgatgctgg tgttttaaat ggatataaat tgcccgttaa tgtttttaag
     4261 tgttttttac cggtttctgt tttagataac tgattgacaa tatctgatga acttaaaaat
     4321 agggaattac ctccatcatt ggctgatttt actacataca taaccaaata actttccggg
     4381 ttttcactaa aagatgaatc actatgaagt ggacattctc caacatcttc cgagaaagtt
     4441 atatcattat ttattacata gtcatcgccg cgatatttaa tatcccaaaa tatagattta
     4501 aaaccatcgt ctttgtggtt tgtcagcatt cctaattgag atagaaagtc aaataatttc
     4561 ttgtttctcc ggctgtcatc aagatggttt agattaagac cgcgaacaat acaataccca
     4621 atacgctgta ctgtattgtg aatttcttct atcaaactat tattgtttgc agataaaata
     4681 tacttttgat tcataacata ctcctgtcgt gaaatgccgt ctgaaacggt ttgcctgccc
     4741 ttacggcagc agccttgccc tgaacatcgc ctccttcatt tggcgcacgg cctcgggcaa
     4801 tccgaggaaa agtgcctgcg aaatcagcga atgcccgatg ttcagttcgc ggatggcgag
     4861 gatttgggcg atgggggtaa cgttgtgtat ggtcagtccg tgtccggcgt tgacgaccaa
     4921 gcccaaatcg ctggcaaaat gcgcgccgtt ttggatgcgc tcgaactgcc tgatttgttc
     4981 ggcgtggctg cgcgcgtcgg catacgcgcc tgtgtgcagc tcgacaacgg gcgcgccgac
     5041 atcacgggcg gcttggattt gcctgtcgtc ggcatcgata aacaaggaca cgcgtatgcc
     5101 cgcgtcggtc aggattttgg tgaattcggc aattttgtct tgttgcgcca atacgtccaa
     5161 gccgccttcg gtcgtgattt cctgacgttt ttccggcacg atgcacacgt cttccggcat
     5221 cactttaagc gcgttttcga gcatttcttc cgtcaacgcc atttcaaggt tcaggcgcgt
     5281 gcggatggcg tttttgacag caaacacgtc cgcgtctttg atgtggcggc ggtcttcgcg
     5341 caggtgcatg gtaatcaggt cggcaccgtg cgtttcggca accagtgccg cctccacggg
     5401 gctgggataa gtcgtgccgc gcgcattgcg gacggtagcg atgtggtcga tgttgacacc
     5461 taaaagcata atctttcctt ttatttctgc cttcagacgg catttgaagc cgtgccgtcc
     5521 gaagtcggga cggtttcccg ggcggtttct ttgcggtcaa actgccgtat ctgttccaac
     5581 acctgccgcg atttcagccc ctcgggcaac aggtggcgga taaaaagccg tgtgattttc
     5641 aatgcctgtt gcaggctttc ggcagtgcgg aaactgcctt cgcgcaaatc gatcaggctc
     5701 tgcccggggg cgacaacgcc ggcggcgtgc ggcggtacgg caaatccttt tccgacgggg
     5761 aagacggctg tttccgggcg gacaaggtat gtgccgcctg ccgcaatcgt cccgccgtcc
     5821 ccgtcgcggt tcaaatcggg ggcaacgccc aacaggttca gcagccgcca ctcgaaacgg
     5881 cgcaagtcgt cgatataagc ggctttgcag cacaccgcct ccatcacttc cgccaacgcg
     5941 tcgtataact cgggcaacgg atcttcgcgg acggtcaact tcagcatcaa ctcgttcaca
     6001 tacaatccgc tgaacaacgc cctaccctgc ggctgcggcc acccgcctat ccattcggcg
     6061 cgatgtaggg tcttcaactc ttgcgaacca taccaagacg cactcaccgg cacaaacggc
     6121 accaacacgc cgcgcaattc gctctgccgt ttgcgcgcgc ttcttgccaa aagtgccaca
     6181 cgcccgtaac ggcggctgaa cgcttcgacc cgcaggctgc tttcgcgcca gggcgaggaa
     6241 gccaacagga agacgggttc gtggttgact cggtgttcgg acatcatgcc tgaaatgccg
     6301 tctgaacgct gccgccgcta caaaacggcg gtcgggtacg aaccgatgac tttgacgaac
     6361 gaagcgcgtt cgcccaagcg ttccaatgcc gtctgaatct gcgcgtcccg gcggtgtcct
     6421 tcgatgtcga tgaagaacag gtattcccac aaaacggatt tactcggacg gctctcaaac
     6481 ttggtcatgg aaatacccga ctcagtcagc ggttgcagca gcgaggcaac cgcgcctgcc
     6541 cggttgggcg cggaaacggc cagcgaagtc ttgtcgctgc cgcttgcacc ggtttcgtga
     6601 tgtcccatca ccaagaagcg cgtggtgttg ttcggttcgt cttcgatgca ctcggcaacc
     6661 atatcgagtc cgtagatttc cgccgccgtg cgtccggcga tggcggcaac cgtaccgtcg
     6721 tccgattcgg caaccagcct tgcggcttcg gcattgctgg acacggcaat ccgttcggcg
     6781 ttgggcaggt gtctgcccaa ccagtcgttg cactgcgcca acgcctgcgc gtgggaaaag
     6841 actttggcaa tgccttcggt gctgccgttg tttttacgca aaaggttgtg gtggatgcgc
     6901 aaaacgactt cgccgcacgc ctgcaacgcg gtaacggcaa gcaaatccaa cgtgcgcccg
     6961 accgagcctt cggtcgagtt ttccacgggg gcgaccagat agtctgcctg acgggtttca
     7021 acctgcttga agcagttgtc tatggtcgga cacgccatgg tgtgcgcggc gtgtccgaaa
     7081 tgcttaatcg ccgcctgctg ggtaaacgtg ccctgcggcc ccagataggc gatggtcagc
     7141 gggcgttcga cggcgaggca ctcgctcatc acttcccgaa acaggcgtgc taccgattcg
     7201 tcgggcagcg ggcctttgtt caaatcctga atgcggcgca acacggcgac ctcgcgctcc
     7261 ggacggtata ctgcgcccgt gcctttcagt tcgccgatgg cgtgggcgtg ttgcgcacgt
     7321 tcgttgagca ggcgcaggat ttcggcatcg atggtgtcga tggcgttgcg gtggggaagg
     7381 aggagttcgt cgatagtttg ggacataatt tcgcctgaac agtaatttat cgtgcattct
     7441 aacatcaaat gccatctgaa acggtttcag acggcatttg atgttaaagg tattcgctct
     7501 gttcgttttc tttccacgca cgatgtgagc agagccacag aagcgcaatg ccgcaagagg
     7561 tggacgcggt catggttacc gccatcacgg ttgccgaacc gttgtgcaag aaggtcgccg
     7621 ccatacccac gcccgcgccg attaaggact ggaacacgcc caacacggcg ttcgcgctgc
     7681 cgccctcttc tttgaaataa gacataaagc atgcctgcgt gttcgcgccg accaagccct
     7741 gcgtgccgac ggaaaacatc acgcacgcaa ccagcagcca aaacgggggc aacccgaaaa
     7801 acagcacggc ggcgagttgg gacaggttgg cggcaaattg gacgacaatc ccccatagca
     7861 ggatgctttg cggatgcacg ccggttttga gccgccacgc ggtaacgcgg ttgaaaaaca
     7921 tcatcgtgat gatgttgagt gcaaacgccc aagcgtattg atgaggcgta acacggtaaa
     7981 gctgctggta cacgaaggaa gattcggtca gaaaggcgaa catcgaaccg aagctgaatg
     8041 cctgaaaaaa cagataaccc atcgcggcac gggttttcaa tacgcgcttg aaccgccccg
     8101 ccaccagccc gaacacgtcc cgtccgattt tgccgccgac ggcaggcttg ggcaggaaat
     8161 gctgcaccaa accgagcagc accagcgaat acgccgccag aaaaacaaaa atcgcctgcc
     8221 agccgcccaa gccctgcaac aatgcgccga ccatgggtgc gaccagcggc acaaccatca
     8281 aaatgatgcc gataagggca aacatctggg cggctttgcg tccggaataa taatcgcgca
     8341 ccatcgcgcc gacgatgacc acagtcatgc ccgcaccgaa tgcctgcacg acacgcaggt
     8401 tgaggagctg ttcggcactc gaagcaaata cgatggcggc aacggcaagg caatatacaa
     8461 tcagaccggt cagggcgacg ggtttgcgcc ctttgatgtc ggacaccgaa ccgccgacca
     8521 cctgtccgaa cgccgtgccg aacataaaca aactcaggct ctgttcgatg cggtggacat
     8581 ccgcgttcag cgactgcgcc atttcgggaa tcgcgggcag gtaggcatcg atggaaaacg
     8641 gcatcagcgt aaccagcatc gccatcaaaa ccgccatcag tttttcgctc atttcaggat
     8701 aatgggcaga aggcataaat tctcgtccga ttcgcaaaca aaccgcccgg cggcggaaaa
     8761 cacaatacgc ggaaacggaa aattttaaca caccggcaca aacaggcggc aacaaaagcc
     8821 ccggcaaaac cggacgaacc ggaacgatat agtggattaa caaaaaccag tacag
//
LOCUS       DAWHXZ010000071         8868 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000071 DAWHXZ010000000
VERSION     DAWHXZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.071"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(171..863)
                     /locus_tag="WIE35_001038"
     CDS             complement(171..863)
                     /locus_tag="WIE35_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ4153966.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGAPKYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(971..2164)
                     /locus_tag="WIE35_001039"
     CDS             complement(971..2164)
                     /locus_tag="WIE35_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ4153967.1"
                     /translation="MSDQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGVVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="WIE35_001040"
     CDS             complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="WIE35_001040"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ4153968.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3017..3946)
                     /locus_tag="WIE35_001041"
     CDS             complement(3017..3946)
                     /locus_tag="WIE35_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ4153969.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="WIE35_001042"
     CDS             complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="WIE35_001042"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ4153970.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4774..6981
                     /gene="uvrD"
                     /locus_tag="WIE35_001043"
     CDS             4774..6981
                     /gene="uvrD"
                     /locus_tag="WIE35_001043"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ4153971.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FILDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASQEQGVSLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMPLPEIIAGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPDNISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            7338..7877
                     /locus_tag="WIE35_001044"
     CDS             7338..7877
                     /locus_tag="WIE35_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ4153972.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYQEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAENHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHAMFALDYRACGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(8067..8282)
                     /locus_tag="WIE35_001045"
     CDS             complement(8067..8282)
                     /locus_tag="WIE35_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153973.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(8290..8493)
                     /locus_tag="WIE35_001046"
     CDS             complement(8290..8493)
                     /locus_tag="WIE35_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4153974.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8406..8765)
                     /locus_tag="WIE35_001047"
     CDS             complement(8406..8765)
                     /locus_tag="WIE35_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ4153975.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
BASE COUNT         2220 a         2449 c         2202 g         1997 t
ORIGIN      
        1 tttcagttac agcagcttga tccctttccc ttatccaacg ggggaaggct aggatagggt
       61 ggcttgcaaa tatacagaac aagggacaag agccaccctc tctccaaccc tctccctccg
      121 tacgggaggg ggtggattct cgcgggcgaa gcccacgcta cggttagcct ttaccccagc
      181 acaaacaatt cccgcccgtg cgccttcagc caacttttag cattgtcggt atgcggcgtc
      241 agcgtgttca ccaaatgcca aaagcgcgga ctgtggtcgg ggtggcggag gtggcagagt
      301 tcgtggatgc agacatagtc ggcaacgtac ttgggcgcgc cgatcagccg ccagttgagg
      361 cggatgccgg tgtgcgggcg gcatacgccc caaaaggttt tggcgttgct caggtctgtg
      421 gcggtgggcg tcagtcctgt ttcggctgcg tgtttttcaa ggcggggcag caggtattcg
      481 cgggcgcgtt cgttcaacag gcggcgcagg tggtcgattt gtgcggcggt ttcttttcgg
      541 ggaagcagga tttcagacga cgtgatacgg atatggcttt ggctgtgggt atccagcttg
      601 gtctttattc cccgatacca aatccactcg ggtaagtttg ggtgggaaac aggatgcacg
      661 ggcgttttgg caagcgtgtt ccgcaaaatc gtttcgtttg ccgccagcca gtttgctaac
      721 gcgtggtctt gaaaaaaggg tgggacgttg atgctgaccg tctgcatatt gacggggcgc
      781 agaatcagat ttttcttggc actgcgtttg agttcgattt cgatgcacaa gccgtcggaa
      841 agagtatagg tgaagcgttt catagttgtg aataggtttc agaccggata catcgtctga
      901 aacaggaatt ttccatatca ggcggcaaac ttcggataat atacaaaatc aaacatctgc
      961 gctacaaggt tcagccgaac aagccgccga tatatttgct gatggtgatg gcgctgagta
     1021 ctgccatcaa accgaccaca atcacgccgg aaacggtgag ccacagcggg tgtttgtagt
     1081 cgccgacaat tttggttttg taggcggcaa tcagaatcag accgagggaa atcggtaaaa
     1141 tcaggccgtt taatgcgcct acgaacacca gcacctgcgc cggtttgccg atggtggaaa
     1201 atacggcggt ggacacggcg ataaaggcaa taatccattt gtttttattg cgttcgatag
     1261 acgggctgag accggagaag aacgacaccg aagtataagc cgcaccaatc accgaagtaa
     1321 tcgaagccgc ccaaatcacc acgccgaaaa tcagcaggcc gatgtatccc gccgcatatt
     1381 caaacggtgt ggaagcaggg ttgtcgggat tgagctgtac gccttggctg accacgccca
     1441 aaaccgccaa aaacaataca atccgcataa tcgaggcaat caggatcgcc cgcaccgagc
     1501 tttggctcac ttccggcaac gccgatttgc ctttgatgcc tgcgtccagc agacggtgcg
     1561 caccggcgaa ggtgatgtag ccgccgaccg tgccgcccac cagtgtaaca atcgccattg
     1621 catcgagttt ttccggcata aaggtatgca cggcggcatc tgccagcggc ggattcgcct
     1681 gccatgccac ataaaccgtc agcgcaatca ttacgaaacc catcacttgg gcgaatttgt
     1741 ccatcacttt gcctgcttct ttaaacagaa acacaccgat ggcaatcacg ccgctgatca
     1801 cgacaccggt ttccggcgac agtccggtca gcaggttcag acccaagcct gcgccgccga
     1861 cgttgccaat attgaacgcc aaaccgccca tcacaatcag cacagccaag aaatagcctg
     1921 cgccgggcaa gacctgattg gcaatatcct gcgcctgttt ttcggaaacg gcgacaatcc
     1981 gccaaatatt gagctgcgcc ccgatgtcga gcagaatcga gagcagaatc acaaagccga
     2041 aacttgccgc cagtgcttgg gtgaaggtgg cggtttgggt cagaaagccc gggccgatgg
     2101 cggaagtcgc catcaggaat gcagcgccga ttaaggcatt tctgcggttt ttttgatcag
     2161 acataatcgc ttatcctcta taaaattggt tgttgctgtg tttgggcgaa acctgcggtt
     2221 ttagctacgc agaaactcgc tttgctcgtt ttggcgaaac ctgcggtttt cagacggcct
     2281 atgaactgtt tttcaagcag aaactttgat gcctgccgcc agtagttcct gccggatttt
     2341 ttcggcaaac accacggcgt gcggcccgtc tccgtgcaga cagatgctgt cggcttgcac
     2401 ggcaaccagg ctgccgttca ctgctttgac ccgcccgtcc cgcaccatct gcaatacttg
     2461 ggcgatggct tcttcgtcgc tgtcaacctg cgcatcgggg cggctgcggg gaaccagcgt
     2521 accgtcgggc atatagcggc ggtcggcgaa tacttcggaa atcacaccca agcctgcggc
     2581 ttttccggct tccaagagca ggctgccgga aagtgccatc aatttcaatt tcgggtcgaa
     2641 atccgccaca attcgggcaa cggtatccgc cagcgcacgg tttttcgccg cttggttgta
     2701 cattgcgccg tgcggtttga cataagccat ttccaaaccc tgatcacggc acaaggcctg
     2761 caatgcgccc aactggtaat tcagacacgc ccgcaaatcg gcttcggaca gattcatttc
     2821 ggtacggccg aagttttccc gatcgggata gccggggtgt gctccgatgc gcacgccgtt
     2881 ttgttgggca tacgccaatg ccgcccgaat atcggcaatg ctgccggcgt gttgggcgca
     2941 ggcgatgttg gccgaagtaa tcagctgcaa caaggcttcg tcgctgccgc agccttcggc
     3001 gagatcggcg tttaaatcaa cctgcttcat gggtgattct ccgtatttgg ttcagatagg
     3061 cttggttttt gcgccgcagg gcggtggctt ctttcaagcc gattattttg aatttgactt
     3121 tgctgccgaa gcgcacctgt gccagcctgc ccaaatcggc ggcggcaacg gtagcgattt
     3181 tcggataacc gccggtggtt tgtgcatcgg ccagcaggat aatcggtttg ccgccgggcg
     3241 gcacctgcac ggttcctgcc tgaacagcgt gggacagcat ttccaaaggt tgcgacaggg
     3301 tcagcggctg tccgtcgaag cggtagccca tgcggttgct atcgctttgc agcgtccacg
     3361 tttcccgttc cagattcaga cgcccttttt cactgaaagc ggcatattcc gacgaaggaa
     3421 caaggtggac ggtatcggta aacggtatcg gggcaatgcc gactttggac aattcctgcg
     3481 cacctttgcc gatggggaga taatcgcctt tttgcagcat tctgccctga tggccgccga
     3541 aaccggcttt caggtcggta cttctcgaac ccatcacttc cggcacatca aatccgcccg
     3601 ccacgcacac atagccgtac atgccctgca cggcacgcac cattttcaag gtctgccctt
     3661 tgcgggcggt ataacgccaa tacgaataga ccggttcgcc gtccaattcc gcctgataca
     3721 cggcaccggt gagacaaaac ggcgtatccc gttcaaacac cagcattatc ccgcccaaag
     3781 cgatttcgat tgcggccgtg ccttcgtcgt tgcccaataa aatattaccc gccgccaaag
     3841 caaccgtgtc catcgcaccg gcatgaccga tgccgtaacg ccggtgtccg tagcgtccgg
     3901 tatcctgaat atgcgccggt gcctgcactg ccgaaacgtg aatcatggct caatcctttc
     3961 tgcaacaaag cggacttggt cacccgccgc cagcagggtc ggcggattca aatcggctcg
     4021 gaacaagggt aattcggttc tgccgataat ctgccagccg ccgggcgaag cgaacggata
     4081 cacaccggtc tgactgccgc cgataccgac cgaaccggca ggaacggacg ttctcggcac
     4141 ggcacggcgg ggcgtgtgca atgcttcggg caagccgccc agataaggga aaccgggctg
     4201 gaagcccatc ataaatacgg tataagtttg cgccgtatgg cggcggacga tttcggaaat
     4261 aaccgtctga tggaaagcag cgacttccgc caaatccggg ccgtattcgc cgccgtagca
     4321 gacgggaatt tccaccagtt tgccctgatg gtctgtaacg gcggtgtgtt cccacacata
     4381 ttgcaattca tcggcaagcg tcgccaaatc ggtatcgaaa cgggtaaaca cggtcagatt
     4441 gttcatgccg accaccactt cctcaatcct gtcgtgctgc ccgagcgcag cggcaaacgc
     4501 ccacaacttt tgctgtttgc ccagttcgga aggcgcattc agtcggcaga ccaaagcgga
     4561 ttcgctgatt ggtgtgatct ctattctcat ttgttgttca ttttggttat gttttaatga
     4621 atctatatgc aggggcggcg gtttgtcaat atcttctgtg ctgcatcatc aaaccgtcga
     4681 ttggaaaagt gctgccctgc cgctgcactt tttcagacga ccttaaaccg tttctattaa
     4741 aatagcgcat tccacttttc agacggcatc cttatgtttc ccgaccaatc cgcccccaac
     4801 ctgctgcaag gcttgaatcc cgaacaactc tccgccgtaa cctggccgcc gcaatccgca
     4861 cttgtgctgg cgggcgcggg cagcggcaaa acgcgcgtgc tgaccacgcg catcgcatgg
     4921 ctgttgcaaa ccggtcaggc aagcgtgcac agcattatgg cggtaacgtt taccaacaaa
     4981 gccgccaaag aaatgcaaac ccgtttgggc gcgatgattc ccatcaatgt ccgcgccatg
     5041 tggctcggca cgttccacgg cctctgccac cgctttttgc gcctgcacca ccgcgacgcc
     5101 ggtctgccgt cttcctttca aatcctcgac ggcggcgacc agctttccct catcaaacgc
     5161 ctgctcaaaa gcctcaacat cgccgaagaa atcatcgcgc cgcgttcgct gcaaggcttt
     5221 atcaacgcgc aaaaagaatc cggtttgcgc gcttccgtct tgagtgcgcc cgatccgcac
     5281 acaagccgca tgattgaatg ctacgccgaa tacgacaaaa tctgccaacg cgaaggcgtg
     5341 gtcgattttg ccgaactcat gctccgcagc tacgaaatgc tgcaaagcaa cgaaatcctg
     5401 cgccagcatt accaaaaccg cttcaaccac attctcgtcg acgagttcca agacaccaac
     5461 aaactgcaat acgcctggct caaactcatg gcgggcggaa acgcagcggt atttgccgtc
     5521 ggcgacgacg accaaagcat ttaccgtttc cgtggcgcaa acgtcggcaa catgaccgcg
     5581 ctgatggaag aattccacat cgacgcgccc gtcaaactcg aacaaaacta ccgctccgtc
     5641 ggcaacatcc ttgccgccgc caatgccgtg attgaaaaca acgacgaacg actcggcaaa
     5701 aacctgcgca ccgacgccga agcaggcgac aaaatccgct actactccgc ctttaccgac
     5761 ctcgaagaag cccggttcat cttggacgaa accaaagccc tcgaacgcga aggctgggat
     5821 ttggacgaaa tcgccgtcct ctaccgtagc aacgcccaat cccgcgttat cgaacaaagc
     5881 ctgttccgca gcggcatccc ctacaaaatc tacggcggct tgcgctttta cgaacgccaa
     5941 gaaatcaaac acgcgcttgc ctatctgcgc ctcgccgtca atcccgacga cgacaacgcc
     6001 ctcttgcgcg tgattaactt cccaccgcgc ggcatcggcg cacgcaccgt cgaaaacctt
     6061 cagacggcat cgcaagagca aggcgtttca ctttggcagg cagcctgcaa tgccggcgca
     6121 aaagccgcca aagtcgccgc cttcgtccgc ctcattgaag ccctgcgcaa ccaagtcgga
     6181 caaatgcccc tgcccgaaat catcgccggc atcctcaaag acagtggctt gaccgaacac
     6241 taccgcaccc aaaaaggcga caaccaagac cgcctcgaca accttgacga actcgtcaac
     6301 gccgccatcg agttcaaacc cgaagacagc aactttgaaa ccctgcccga caacatttca
     6361 gacgaccccg ccttccccat cctcgctttc ttgagcaacg ccgccctcga atccggcgaa
     6421 aaccaagcag gcgcaggcga aaaagccgtc caactgatga ccgtccacgc cgccaaaggc
     6481 ttggaattca acgccgtctt cctcaccggc atggaagaag gccgcttccc cagcgaaatg
     6541 agccttgccg aacgcggcgg cctcgaagaa gaacgccgcc tcatgtacgt cgccatcacc
     6601 cgcgcccgca aacgcctcta catcaccatg gcgcaacaac gcatgctgca cggacaaacc
     6661 caattcggca tcgtctcccg cttcgtcgaa gagatcccac ccgaagtatt gcactacctg
     6721 tccgtcaaaa agaccgccta cgacagctac ggcagcccgc gccaaaccgc cgcatccaaa
     6781 gacaaaatca tcgacgacta caaacagccc caaacctacg caggtttccg tatcggacaa
     6841 aacgtccgcc acgccaaatt tggcaccggc gtgattatcg atgccgcaga taaaggcgaa
     6901 tccgcccgac tgaccatcaa tttcggcaaa cagggcgtga aagagttgga caccaagttt
     6961 gcgaaattgg aagagatgta aatttgaaat gcaggctgga tactcgtatc cgacaaaaat
     7021 atttgacgcg tctatcgttt ccgaaaaacc gctgttggaa aatgtcggat tcgagaatcc
     7081 gacctatggc aaaaaactgt agcaaggaca aggcaaaagg ccgtctgaat ccggtaacaa
     7141 caaattttgc cgaaacctcc gccgtcattc ccgcgcaggc gggaatccag acctttcggc
     7201 acggaaactt atcggataaa aggtttcttt agattccacg ttctagattc ccgcctgcgc
     7261 gggaatgacg gacggtaaaa gccgggtata gatacctaac ccttgtttat agctgaaaat
     7321 caataaggaa gaacattatg tcccaagttt ttaaagattt tgatttgtcc tccgtatggg
     7381 aatccgacag ttgggcagat gaaaactacc aagaagcccc gtttacccct gaaattttgg
     7441 ctgccgtaga aagtgaactg ggctataaat tgccgcaaag ttttattgaa ttgatggcgg
     7501 tacaaaacgg cggaatattt gtcaaaaact gttttccgac cacgcagaga aattcgtggg
     7561 cggaaaatca tgtgcaaatt tgcgaggtat cgggcatcgg ctttgaaaaa gaagggagtt
     7621 tgtgcggcga aacagggcaa aaactttggc tggaagaatg ggaatatccg cccatcggcg
     7681 tgtattttgc caacgacccg tcaggcggtc atgccatgtt tgccttggac tatcgggcgt
     7741 gcggcaaaga cggcgagccg aaagtggtgt ttgtcgagca agaatcggat tttgaaatcg
     7801 tcgaacttgc ccccgatttt gaaaccttta tccgcagctt gcggcatgaa gatgagttta
     7861 ttgacgagga aatataaaac ggcggttgaa aaaccggaat catcaaggga aaacgggcga
     7921 aataacggat aatcgcttga atccgtaagg aaaacggttt ggtggaacgc gccatccaag
     7981 acctttgcaa agaaaaactg tctccgacag cattgacatt attaacagaa cttatcaagt
     8041 ttggagctat ctcaaatata attcggttat ccttttgtat ccattaaatc atatgcttca
     8101 attaattgtt gttctagctc ttgtaccaat tttgaattgc gaattcctga cacaatctca
     8161 aattcttctg catctatgca aacacctgca taaatttcaa taacaaggga acgcaataat
     8221 tgaagctctt ctcttgttaa agaaataata atgtcatcac ctgtgtaatt gattatattc
     8281 ataataattt tatttttgtt tgtcaaagta agttttgcct aaggttggtc taaatgcagt
     8341 tccaccatct tttgaatttg ggtctctgat tacaattgct ccagacttat catcccaaat
     8401 tgctcttatg tgtttggatt gtaatcttcg aattcccaag aaaaaaatcg taataagttt
     8461 gaaagtgtca aatcccaagt ttcttttgag caatattcta atattttatc aatttcactt
     8521 ttaataatct tatgatcaaa ctgttctaat attaatgcat tagaccaaaa aaaaccttct
     8581 ttattacaat gatgggaaat ccatttagga gaacaaatgc aaagtgaaaa aatagatgag
     8641 ccttgttctc cttcgattcc gatatccaaa tctatccatc tatggaaatt atctggaatt
     8701 tcgggggtaa atttttcaaa atcaatatca tataaattta tgctttttaa atccaattta
     8761 atcattaggg ctgtcctaga taaataggga aattcaaatt aagttagaat tatccctatg
     8821 agaaaaagtc gtctaagccg gtataaacaa aataaactca ttgagcta
//
LOCUS       DAWHXZ010000072         8857 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000072 DAWHXZ010000000
VERSION     DAWHXZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8857
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.072"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..157)
                     /locus_tag="WIE35_001048"
     CDS             complement(<1..157)
                     /locus_tag="WIE35_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ4153976.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKDWLPGGFLGVDIFFV
                     ISGFLMTT"
     gene            complement(168..272)
                     /locus_tag="WIE35_001049"
                     /pseudo
     CDS             complement(168..272)
                     /locus_tag="WIE35_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(524..1615)
                     /gene="ychF"
                     /locus_tag="WIE35_001050"
     CDS             complement(524..1615)
                     /gene="ychF"
                     /locus_tag="WIE35_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ4153977.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVSEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(1728..3404)
                     /locus_tag="WIE35_001051"
     CDS             complement(1728..3404)
                     /locus_tag="WIE35_001051"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ4153978.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKANGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALEKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            3579..4010
                     /locus_tag="WIE35_001052"
     CDS             3579..4010
                     /locus_tag="WIE35_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ4153979.1"
                     /translation="MGSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGHNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            complement(4241..4936)
                     /locus_tag="WIE35_001053"
     CDS             complement(4241..4936)
                     /locus_tag="WIE35_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ4153980.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEEALGDGSAYGVNIAYSPEPEGGLETAGGIARALPLLGDAPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHHPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(4954..5454)
                     /gene="hpaC"
                     /locus_tag="WIE35_001054"
     CDS             complement(4954..5454)
                     /gene="hpaC"
                     /locus_tag="WIE35_001054"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ4153981.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTNLSPEERFAY
                     HIWHYGKTGQPEIEGALAHLHGHIVGQHEIGTHFVFYVRLDEIKNCVGKRPALLYFRR
                     QFRSLD"
     gene            complement(5569..6009)
                     /gene="nadR"
                     /locus_tag="WIE35_001055"
     CDS             complement(5569..6009)
                     /gene="nadR"
                     /locus_tag="WIE35_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ4153982.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(6352..6861)
                     /locus_tag="WIE35_001056"
     CDS             complement(6352..6861)
                     /locus_tag="WIE35_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ4153983.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAILVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(6940..7015)
                     /locus_tag="WIE35_001057"
     tRNA            complement(6940..7015)
                     /locus_tag="WIE35_001057"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6980..6982),aa:Lys,seq:ttt)
     gene            7226..8305
                     /gene="apbC"
                     /locus_tag="WIE35_001058"
     CDS             7226..8305
                     /gene="apbC"
                     /locus_tag="WIE35_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ4153984.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPLTGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICTNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
BASE COUNT         2052 a         2534 c         2280 g         1991 t
ORIGIN      
        1 tcgtcgtcat caaaaagcct gaaatcacaa agaatatatc gacaccgaga aacccgcccg
       61 gcagccaatc cttttcgata tggaacacga tgacggacaa gacggcggcg gcgcgcaatg
      121 tgtcgatgtc cgggcggtag ggtaaggctt ggctcataat gtttttatag tggattaaat
      181 ttaaaccagt acggcgttgc ctcgccttgc cgtactatct gtactgtctg cggtttcgtc
      241 gccttgtcct gatttaaagt taatccacta tactcgaaac gcggcggcgc aaatgccgtc
      301 tgaaaggtca ttttgtacgg gatattcgga atgccggacg gatttttgca acagcggggc
      361 aggcggtttg ttttgcagga atcggggagg gcaaatcgga aatgcggacg ggattttatt
      421 ttgatgcagc cgcattccgg cggtatggaa acgccgaaaa ccaccaaaac cggcttcaga
      481 cggcatttct ggcaaagccg tctgaaaacc tgccgcattt gggttacacg ttaaacaaaa
      541 agtgcatcac atccccatcc tgcacgacgt attccttacc ttccacacgc attttgccgg
      601 cttctttggc tttggcttcg ccgccgagcg agacaaagtc gtcgtaagaa atgacttggg
      661 cgcggatgaa gccgcgttcg aagtcggtgt gaatcacgcc ggcggcttgt ggggctgtgt
      721 cgcctttgtg tatcgtccac gcgcggactt ctttgacgcc ggcagtgaag taggtttgca
      781 gccccaaaag gtcgtaaccg gcgcggatca ggcggttcag gcccggttct tccaagccca
      841 tttcggcgag gaactcggct ttttcaccgt cttccaactc ggcgatttcg ctctccatcg
      901 cggcgcacac ggcgacgacg ggggcgtttt cttttgccgc caattctttc aggcggtcga
      961 ggtgcgggtt gttttcaaaa ccgtcttcgg aaacgttgcc gacgtacatg gcaggtttgg
     1021 cggtcagcag gaacagcggt ttgagcatcg cgcgttcttc cgcgtccaaa ccgaaggaac
     1081 gcacgggttt gccttcgtcc agatgcggca gcagtttttt gcacaaatcg accagctttt
     1141 gcgcgtcttt gtcaccggaa cgggcgcgtt tttcttcgcg gacgatggct ttttccacac
     1201 ttgccaagtc ggcaagcgcc aactctgtac cgatggtttc gatgtcggca atcggatcga
     1261 ctttgccgga aacgtggacg atgttgtcgt cgtcaaagca gcgcacgaca ttcacaatcg
     1321 catcggtttc gcggatgttg gcaaggaact ggttgcccaa gccctcgcct ttgctcgcgc
     1381 ctgcaaccaa accggcaata tcgacaaatt cgacgatggc aggctgcatt ttttgcggat
     1441 tgacgatttt tgccaattcg gccatacgcg gatcggggac ttcgacgatg ccgacgttgg
     1501 gttcgatggt acagaaagga tagtttgccg cttcgatacc cgattgggtc agcgcgttaa
     1561 aaagggtgga tttgccgacg ttgggcaaac cgacgatgcc gcatttcaaa ctcatgtttt
     1621 ttcctgaaaa tagagaaatt taacggcgga ttatagcata ccgccgcccg cgttccgaaa
     1681 aatgccgtct gaaacggctt cagacagcat ccggtttcaa aaaccgttca gaacaagccg
     1741 tgaatcacgc cgtgttcgtc cacatcgatt ttctcggcgg ccggaacttt cggcagaccc
     1801 ggcatcttca tcatattgcc gcacagcgcg acgatgaagc ccgcgccggc ggaaacggtg
     1861 atgccgcgca cggcgatgcg gaagccttcg gggcagccca acagtttggc gttgtcactc
     1921 aaagagtatt gggttttcgc catgcagatc ggcattttgt ccaagcccag tttttccagt
     1981 gaagcgattt cggcagacgc ttccgcgctg aaatcaacat cttccgcgcc gtacactttt
     2041 tgggcaatcg cacggatttt gtctttgatg cccaactcga catcgtaggc gaaaccgaag
     2101 ttattggttt gactttcaat ggcgttgacg actttgcgcg ccaaatccgc gccgcccgca
     2161 ccacctttgc cccacacttc ggtcagggaa acttcaacgc cgtgttcggc acaggctttt
     2221 tcaatcatcg ccaactcggc atcggagtcg gacacgaagc ggttgagcgc aacgacgacg
     2281 ggcagtccga atacgttttt caggttggaa atgtgtttca gcaggttggg caaacctttt
     2341 tccaaagcgt ctaaattttc ttcgccgagg ttggcgcgtt ccacgccgcc gttatatttc
     2401 aacgcgcgga cagtcgccac gacaacagcc gcatcaggtt tcaaaccggc aaggcggcat
     2461 ttgatgtcgc agaatttttc cgcgcccaag tccgcgccga agcctgcttc ggttacggcg
     2521 taatcggcaa ggtgtttcgc cagacgggtt gcggttacgg agttgcagcc gtgggcgatg
     2581 ttggcgaacg ggccgccgtg tacgaaggcg ggcgtgcctt cgatggtttg caccaagttg
     2641 ggcttaatcg catctttaag caatgccgcc atcgcgccat tcgctttcaa atctttggcg
     2701 taaacggggc tgccgtcttt ggcgtaggcg acaaggatgt tgcccaaacg ctctttcaaa
     2761 tcgctgatgt ctttggcaag acagaatacc gccatcactt cggaagcaac ggtaatatcg
     2821 aaaccgtcag gacgcatcac gccgtcaaca ggcttgccca tgccgtcgat gatgttgcgc
     2881 aactggcggt cgttcatatc gaccacgcgc cgccacagca cgcgtttggg gtcgatgttc
     2941 aactcgttgc cttggtagat atggttgtcg agcatcgcgg caagcagatt atttgccgca
     3001 ccgatggcgt gaaaatctcc ggtgaagtgc aggttgatgt cttccatcgg caaaacttgg
     3061 gcatagccgc cgcctgccgc gccgcctttc acgccgaaca ccggccctaa agaaggttcg
     3121 cgcagggcaa tcacggcatc tttgccgatg tggcgcaacg cgtccgccaa accgatggtt
     3181 acggtggttt tgccttcgcc ggcaggagtc gggttgatgg cggttaccaa aatcagcctg
     3241 ccttgttttt gcggcagttt aaacgcttcg gcaggattga ttttggcttt gtaatgaccg
     3301 taaggctcaa tgttgtcggc gttcaggccc agcttggcgg caatttcgcc aatcgggcgc
     3361 atggtggaag actgggcgat ttcggcatcg gttttgaagc tcatgatttt cctttagaaa
     3421 tgaggaggga cataccgtct gaaagcatca ggcgacaaac aggtggattg aaaataatat
     3481 caggcatatt ataacgttat ccgcaccaaa cccgcagtga aatttttgac gctgcaacaa
     3541 aaataccgtt catattgttc acaatccaag gagaaaacat gggcagcaac gcatggctgt
     3601 tttgggcatt ggcatcggca ggcttcgcct cattgaccgc cgttttcgcc aaaatgggct
     3661 tgcagggtat agattcagat ttcgctacct tcatccgcac cttggtcatc ctcgccgctt
     3721 tggtactgtt tttgacctac accggcaaat ggcagggcgt gaacggcttt acggggcaca
     3781 actggacatt ccttatccta tccggccttg ctaccggcgc atcttggctt gcctacttca
     3841 aagccctgca actgggtaac gcctcgcaag tcgcccccgt cgataaattc agcctggttc
     3901 tggtcgcgct gatggcggtg gttttcttgg acgaacgccc gaacacgcag gaatggatag
     3961 gcttggggct ggtaacggcg ggcgtgttgg tgctggcgtt gaaacgttaa accgaatccg
     4021 ccataccgcc tgaaaccggg ttttgacaat ctgacgggcg gcggtctgaa caaagttacc
     4081 caataaaatc cgacaccgcg ccgacggcaa accgccgcgc ttcagatggc atttgttgga
     4141 cggaaagccc cgaacgccct attttcacgc ctccacgaaa atatgccgga catccggtcg
     4201 gaaccgttcg gcagccggct cggcaagcca ccgttttatt tcacttccaa gcccctgcaa
     4261 gggcttgagc ctctttcaga cggcataccg tgccgacatc cagccacaag cccgtatgct
     4321 tctgaccgct cacgcggttt tgccgcattt cgttccgcaa tacgggcgcg agtttcgcca
     4381 cactgcccgc ttcgattccg tcaaacattt caggacggta aatacccacg ccgctaaatg
     4441 tcaatccgtt gccgccattt acttccggcc gcacgctgcc gtcgggcagc agggaaaaat
     4501 cgccgtcagg atggtgcggg ggattttcca ccagccacag atgggcggaa atgtgctcgg
     4561 gcagggacga tgccgcctga aacgcggcgg taaaatcgat gtcggtcaac acgtcgccgt
     4621 tgaccaccaa aaacggggca tcgcccaaca gcggcaatgc ccgtgcgata ccgcctgcgg
     4681 tttccaagcc gccttcaggt tcgggcgaat aggcgatgtt cacgccataa gccgagccgt
     4741 cgcccaaagc ctcttctatc tgccgaccca gccaagcgtg gttgatgacg atttcggtaa
     4801 accctgcctg cttcagacgg cataggtgcc aaccgattag aggcttaccc gccacatcga
     4861 gcagcggctt cggagtggta tcggtcaaag ggcgcatacg ctcgccgcgt cctgccgcca
     4921 gtatcatcgc tttcatatat atctgtccga atatcagtct aaagatctaa actgccgtct
     4981 gaaataaagc agcgcgggac gtttgcccac gcaattttta atttcgtcga gcctgacgta
     5041 aaacacaaaa tgtgtgccga tttcatgttg gccgacaata tgcccgtgca gatgcgccaa
     5101 cgcgccttct atttcaggtt gtccggtttt accgtaatgc caaatatgat aggcaaaacg
     5161 ttcttcgggt gaaagattgg tcaatccggc aaaatgttcg gccacatcct gatgttcgtc
     5221 tgccaacgta ttgatgcaca ggctgccgtt ttccgacagg atcggaatga ttcgcgcact
     5281 ccggttgatg cacagcatca cggtcggcgg ctcgtcggta accggcgcga ccgccgtcat
     5341 tgtaatgccg taacgccctg ccgcaccgtc tgtcgtgatg acatgaacgc ctgccgcgca
     5401 agatgccatc gcatcacgga acgaagtttg aaaagttttc tgcaaatccg ccatttttcc
     5461 cctttaaact gtcccctata taagaatgct gcacacaagg caccccccat gtgcagcagt
     5521 tctgattcaa aaagccgtcg gtcggacatt tccgcgcgtt acggcgtatt acgagttcaa
     5581 cgcatcctcg attttggcaa gttctgccaa caggtcttta agcagcagca ttttctcgcg
     5641 gcccagcact tcctcgatag cgtcgtaacg ctcgtccact tcttcgccga tttcctcata
     5701 cagcttctcg ccctcggcag tcagcttcag aaaaacacgt cgttggtcgt tggaaggttt
     5761 caggcggaca accaaacccg ctttttcaag gcgggtcagg ataccggtca ggctggggcg
     5821 caaaatgcac gcctgattcg ccaaatcttg aaagtccagc gtgccgtttt ccgccaaaag
     5881 acggataatc cgccattgct gatcggtaat attcgcctga ttcagaatag gcctgaattg
     5941 ggtcatcagg gcttcccttg cctgtatcag actgatattg atagacgcat gttttgattg
     6001 ggtaggcatt gttcaagtct ccaagttatc gaaaatcaaa ctttcaaacc gtcgggaaag
     6061 cctgtgggcg taatttttga tgcaaccgtt atataacaaa acgaacatat agcaacaata
     6121 cgctataaac cgcatcggac gactgggtat aaaagacttt aattccgata atcctatcta
     6181 aaaatatttt aatagttata tcttagtcta ttttccctac aatctcaaca aagtattaca
     6241 tttactgtcg aaagaacttt cttccagaaa aaacaaaagc cgccgcatca gacgagcaag
     6301 gtatatgccg tttgattctc tacatagcgt aaagtttaac aaaaacaaaa gttaaccgaa
     6361 aacatccgcc tgaaaaattc gtgcgcgcaa gccccaataa ctgctgattc ccgtcgtata
     6421 gtgaaccatt ttcccatttt tgaccaaaac gacggcaggc gttgcgacaa tccgccaaga
     6481 ccttgccaaa cccccgtcct catcgttgac cgtcggaaag cccaagcccc gctttgccat
     6541 atacgccgcc acttcggacg cgctgccgga acgtaccgcc acgccgacga ccggaacgcc
     6601 gtccgccgcc aaatcatcga ttatcggcga ctgataacgg cacacgccgc accagcttcc
     6661 ccaaaaatac accaaaatcg ccttatcccg gctaaactgt cccaaagtca gccgctgccc
     6721 cgacagcagg gtcaaaggcc gccctgccgc accggccggc tcttcgggct tgcgtatcca
     6781 atccaaaaac agcgacacca ataaaaacac caatgccgtc tgaacggcaa atttgatgcc
     6841 cgaaagcagt ttctttttca tacgctctct caaacggtac gcccgcgcaa ccggcaggca
     6901 aacaaaaagc caagtctcaa aacttggctt ccggttatct ggtgggtcgt gagcgattcg
     6961 aacgctcgac caacggatta aaagtccgct gctctaccgg ctgagctaac gacccgataa
     7021 gccgtgcatt atacagcacc atcctacctc gtcaagcaaa ttttacaggc ttaattgcag
     7081 accactgttt gcacgggata ttttgacaac ggattttcac aatccgccgc ataccgtgta
     7141 aaagttcgca caaggaaaag caaaccgccc gaaatcaatg tacactttcc gcccgtttcc
     7201 caaactgcac acagaaacac acattatgaa catacaaaac atccgcaccc tcctcgacac
     7261 cgtcgccgtt ccgaatacgg cacgcacgct cggcggcgaa aaggccgtcc gttcggtcga
     7321 acagcgttca gacggcatcc atatcgccct gcatttcggc ttccccgtcg cgcacattgc
     7381 ctcagaaaca gccgaccgca tacaggaaat cctgatgccg ctgacaggcg acacacacat
     7441 ccatctgtcc atggacactg aaatcggcac acacaaagtc cagcccggcg ttaccactat
     7501 taaaggcgtg aaaaacatca tcgccgtcgc ctctggcaaa ggcggcgtgg gcaaatcaac
     7561 caccaccgcc aaccttgccg ccgcaatggc gcgcatgggc gcgcgcgtcg gcgtactcga
     7621 tgccgacctt tacggcccga gccagcccac gatgctgggt gtggacgacc gcaaacccga
     7681 tcagaaaaac caaaaactca ttcccgtcga atcttcagac ggcatacagg tcatgtctat
     7741 cggctttctc gtcgataccg accaagccgt cgtctggcgc gggccgatgg tcagccaagc
     7801 cttgcagcaa ctgatgtttc aaagcgagtg ggacgaagtg gattatcttt tcatcgacct
     7861 gccccccggt accggcgaca tccagctcac gctgtcccaa cgcatccccg ttaccgggtc
     7921 cgtcatcgta accacgccgc aggacatcgc gctgatagac gcgcgcaagg ctgtggatat
     7981 gttccgcaag gtcaacattc ccattttagg cgtattggaa aatatgtccg tccacatctg
     8041 caccaactgc ggacacagcg aagccctgtt cggcacggac ggcggcaaag atttagccgc
     8101 acgcctcaac gtccccctgc tcggacagct tcccctaagc ctgcccgtgc gcgaagcaat
     8161 ggacggcggc acaccggcgc aactgttcga cgaacacccc gccatcgccc gaatctacac
     8221 cgatgccgca ttccaaatcg ccctgggcat tgccgacaaa ggcaaagact tcagcagccg
     8281 gttccctaaa attgtcgtcg aataaagccg cgtccgaaac cgcaacagca atgccgtccc
     8341 aagccccgcg cctgccggcg ggcttcatag tcctctctcc ccgtgggaga gggctggaga
     8401 gagggcaaca agccgcaagg cttgtattgg ggcggttaag ctgttgggaa agattgccgc
     8461 aacttggaga atgccctctc cccggccctc ccccacaggg gagggagcgg attgcggcag
     8521 gttttgcggt tgcaggcggt ttgaaaaaca gattggattt acaaccgttg atgcaggtcg
     8581 tctgaaaagc aaaaagcagc ctgcacaacc tgattagttt acaaaaccct taaccccaac
     8641 agcctccccg tcctctctcc ccgtgggaga gggctggaga gagggcaaca agccgaaagg
     8701 cttgtattgg ggcggttgag ttgttgggaa aggttgccgc aacttggaaa atgccgcctc
     8761 cccggccctc ccccacgggg gagggagtgg gtttcggcag atttttcggt tgcaggcgat
     8821 ttgaaaggca acttagattt gcagctgttg tttcagg
//
LOCUS       DAWHXZ010000073         8845 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000073 DAWHXZ010000000
VERSION     DAWHXZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8845
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.073"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(43..237)
                     /locus_tag="WIE35_001059"
     CDS             complement(43..237)
                     /locus_tag="WIE35_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ4153985.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(227..2308)
                     /locus_tag="WIE35_001060"
     CDS             complement(227..2308)
                     /locus_tag="WIE35_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ4153986.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            2784..3164
                     /locus_tag="WIE35_001061"
     CDS             2784..3164
                     /locus_tag="WIE35_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ4153987.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDGGGRGHMPPVQNQAGTDDF
                     RAFSCENGLSVHVRRLDGGRIALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            3819..5252
                     /locus_tag="WIE35_001062"
     CDS             3819..5252
                     /locus_tag="WIE35_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ4153988.1"
                     /translation="MNEYSQLIKHPDISLPPVSDGIKVDNPATGETLAFVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADVLWRWYFLIKENKEALARLMTMEQGKGLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALAVLAYEAGVPQDLLPVVSGSASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGTLASKF
                     RNSGQTCVCTNRVYAQSGIYEEFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            5361..6149
                     /locus_tag="WIE35_001063"
     CDS             5361..6149
                     /locus_tag="WIE35_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ4153989.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            6517..8073
                     /locus_tag="WIE35_001064"
     CDS             6517..8073
                     /locus_tag="WIE35_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ4153990.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADSQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQQEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8090..8836
                     /gene="surE"
                     /locus_tag="WIE35_001065"
     CDS             8090..8836
                     /gene="surE"
                     /locus_tag="WIE35_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ4153991.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVEPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAVFWHAD"
BASE COUNT         2065 a         2225 c         2557 g         1998 t
ORIGIN      
        1 ccgtctgaag cggcgttcag acggaatttg tttcaagcag gcttaacagc agcgtccgcc
       61 gtttgcgccg cagcggcgtt tacggcaata gtcttgaaac tgcagcttgg tcatcacggg
      121 ggcgttggga ttatgtttgc gctgctgtgc aacgtagttt tcataatcgg gcacgcctgc
      181 catcaagttt gccgtcagct tgatggtttt ccaccaagac gcgagcttat gcttcacttt
      241 gtgcctccgg ctgtttgccg tcgcggtaca ccgccgggat ttctttggcg gtcggccagc
      301 cgactttgcg tgctttgagg gcggtacgca aaccgtacgc ggcgacaatc acgacaaccg
      361 acaagaagag gatggtcaga ccggcattaa tcttgtcgtt gaagatgatt tgcgccattt
      421 cgccgatgtc tttggcaggc gcaaggactt cgtttttagc caatgcgtcg ctgtatttgc
      481 cggcgtgggc aaggaagctg atgcgcgggt cgctgtggaa cagtttttgc aggccggcgt
      541 agcaggttac gaacagtacg ccgacggcgg gaacgagtac cacccagaca taacggtcgc
      601 gtttcatctt aatcagcacc acggcgcaca taatcaaggc tacgcctgcc agcatttggt
      661 tggcgatgcc gaacaaaggc cagagcgagt tgatgccgcc cagcgggtcg gttacgccgg
      721 tgtagagaaa gtagccccac aatgccacgg cgaagaaggt cgcaatcagg ttggcgggga
      781 tggagtcggt gttgccgaaa ggtttgtaga agatgctgcc caagtcttga atcataaaac
      841 gtgcgacgcg cgtaccggca tcgacggcgg tcaggatgaa caaggcttca aacaataggg
      901 cgaagtgata ccagaacgcc atcatcgcct cgcccggaat caggcggctc ataatgtgcg
      961 ccataccgac tgcgagggtg ggcgcaccgc ctgcacggga aaggatggtg ttttcgccca
     1021 cttccttagc ggtgtgcaac agggttgcag catcgacagg gaattgcagc ttggtggtaa
     1081 tcacttcggc ggcggtattg gcatccgtac cgatcagggc ggcagggctg ttcatggcga
     1141 agtacacgcc gggatcaagc gatgcggcgg cggcaagtgc cataatggct acgaaacttt
     1201 ccatcaacat accgccgtaa ccaatcatac ggacgtgggt ttcgttttcc agcattttcg
     1261 gcgtggtgcc ggaggaaatc agcgcgtgga agcccgaaac cgcaccgcag gcgatggtga
     1321 tgaagaggaa cgggaacaat gcgcctgaga ataccgggcc ggaaccgtcg ataaagtggg
     1381 ttacggcagg catttgcaaa gtgggattga cgatgacgat gcccaaagcc aaggccgcaa
     1441 tcgtaccgat tttcaggaag gtggagagat agtcgcgcgg agtgagcagc agccatacgg
     1501 gcaatacgga ggcgacaaag ccgtaaatca taatcgccca agtgagctgg atgccgtcaa
     1561 ggtcgaacca atgtccgatg gaacttttcg ccacatcttc gccgtaaatt accgccagca
     1621 tcagcaaaat aaagccgacg atggaaatct cgccgatttt gcccggacgg atatagcgcg
     1681 tgtaaatacc cataaacagg gcaatcggca tagtggcggc gatggtaaac gtaccccaag
     1741 ggctgtgaac cagtgctttt acgacaatca acgccaacac cgccataatg atgaccataa
     1801 tcatcaaaat accgatggag gcaatcacgc cggggacagt gccgagttcc tgtttcacaa
     1861 tatcgcccaa agacttaccg tcgcggcgca tagagacgaa caagaccatc atatcctgta
     1921 ccgcgccggc aaacaccacg ccgaagataa tccacaaagt accgggcagg taacccattt
     1981 gcgccgccaa aaccgggccg accaaagggc ccgcgcccgc aatcgcggca aagtggtgtc
     2041 cgaacaatac gcctttgtgc gtcggaacgt agtccaagcc gtcgttgtgg cgttctgccg
     2101 gagtcaggcg gttaggatcg agccgcatta cgcggttggc gatgtagagg ctgtaaaaac
     2161 ggtaggcgat gcagtaaacg gatatggcgg cggtgaccat ccataccgcg ctgacctgtt
     2221 cgcctcggct gagggccaga gtggtaaagg atgctaagcc gaccagtacc actatgcccc
     2281 aaatgaggaa ggttttgagt gatttcatcg aataaatcct tatctcacac tgtcggaata
     2341 tgcctgaacg gcgggaaacg gatgaccgcc tgccggcacg ggcacaaacc ggagtctgaa
     2401 tgattgacga agtatgaaac agtgcgcttg tcgggatttg tgccttatgc ccgcatcaaa
     2461 cagtgcaggg atgcgggcgc acaaaacgtt aaacgccgaa taggatttta acgcaaatta
     2521 gcacactgat agcggttttt acttggaatt tggaaaaaat ttacattcct tcgggcggac
     2581 aggcgggttt ggacggcatc gtcaggcaaa aggcggtgcc ggaagacggg taaagaaggg
     2641 gcgcgcaatc cggattattg attcatcgcg ggtaaattcc ggttatcggg cttgtgtgtt
     2701 tgcgcgtccg tttatagtat ggcgttgccg cagcttggaa tcagggcggt tgtttcatat
     2761 cttattttta ttggggagct tttatgaata tcaggttttt cgcgctgacc gtaccggttt
     2821 tgtctttggc ggcctgtgcc gtgccggaag cgtatgatgg cggcggacgc gggcatatgc
     2881 cgcctgttca aaaccaagcc ggcacggacg attttcgggc gttttcctgc gagaacggtt
     2941 tgtctgtgca cgtccgccgt ttggacggcg gcagaatcgc gttgcggttg gacggcaggc
     3001 gtgccgtcct ctcttccgac gttgccgcat ccggcgaacg ctataccgcc gaacacggtt
     3061 tgttcggaaa cgcaaccgag tggcaccaga aaggcggcga agcctttttc ggctttaccg
     3121 atgcctacgg caattcggtc gaaacttcct gccgcgcccg ctaaacgggg cagcctgttt
     3181 gaaccgccgt ccggattttt cgggcggttt tttgtgtcgg atttgttttg gctgtcggct
     3241 ggtttcggcg gttttcgggc gtggcggatt atttttcgtt tcgttccggg tttatcctat
     3301 cggcagcggc tcaatgccaa ctttaaacct gctccgattt cttcagggct gttatccaat
     3361 gataaaatat agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacag
     3421 atagtacgga accgattcac ttggtgctta agcaccttag agaatcgttc tctttgagct
     3481 aaggcaaggc aacgctgtac tggtttttgt taatccacta taatgtttgg atttttgcca
     3541 tactctacca cacgttgcaa ctgcaatctt tgttccttat atattattaa agggctgcat
     3601 tacttttctt tctaaattac cattgtataa aaagtatttt tcttaaacga cagggattgc
     3661 gcccgctcca tattcagaca ccgcccggat gttgcgctgc ccgatcggat gcttcagacg
     3721 gcatcggaag ggtttgcagt tttggaatat gaagatgata atgtccgcga gattgacggc
     3781 atttgaaagc ggttattccg cataccggag gatacgaaat gaacgaatat tcccaattaa
     3841 tcaagcatcc cgatatttcc cttcccccgg tttcagacgg catcaaagtc gataatccgg
     3901 caacgggcga gactttggcg tttgtccgca agacggattc ggacaagctg aaaaacctga
     3961 ttcaaaaggc ggcggcagcg caaaaattat gggcggcaaa aaccgcgttg gaacgcgccg
     4021 atgtgttgtg gcgttggtat tttttgataa aggaaaacaa agaagcactg gcgcgtctca
     4081 tgacgatgga gcagggcaaa ggcctgaccg aggcgcgcgg cgaaatcgat tatgcggctt
     4141 cgtttgtgcg ctggtttgcc gaagaggcgc ggcggattga cggcgatgtg ctgacgagtg
     4201 tgaaagcgtc gcaaaaactg gtcgtgttga aacagcctgt cggtgttacc gccgcgatta
     4261 cgccgtggaa ctttccgtcc gcgatgattg cgcgcaaggc tgcgccagct ttggcagtgg
     4321 gttgcgcgat gatagtcaaa cccgcgtcgc tcacgccttt gagcgcgtat gcgttggccg
     4381 tgttggctta cgaagcgggc gtgccgcagg atttgctgcc tgttgtcagc ggcagtgctt
     4441 cggaaatcgg ccgtgaattt gccacaaatc cgattatccg caaaatcagt ttcaccggct
     4501 ctaccgaagt cggcgcgaaa atttttgccg acagcgcggc agacattaaa aaactcagct
     4561 tggaactggg cggcaacgcg ccgtttatcg tgtttgacga tgccgatttg gacaaagctg
     4621 tcgaaggtac acttgccagc aagttccgta atagcggtca gacctgcgtc tgcaccaacc
     4681 gcgtttacgc tcaatccggc atttacgagg aattttgccg caaattgagt gaaaaagcag
     4741 ccgcgctcaa attgggcaac ggcttggagg atggcgtaaa ccaagggccg ctgattgagg
     4801 aaaaagcggt agaaaaagtc gagcagcaca tcgccgacgc gctctcaaaa ggcgcggtct
     4861 gtctgaccgg cggcaaacgc agcgcgttgg gcggaacgtt ttttgagccg accgtcttaa
     4921 gcggtgtaac ggcgcaaatg gcggtagctc gcgaggaaac tttcgggcct ttgtgtccgg
     4981 tattccgttt tgaaaccgaa gccgaggtca tcgaggctgc gaacaatacg gaatacggtt
     5041 tggcagctta ccttttcacc gccgacaccg cccgccaatg gcgcgtcggc gaagccttgg
     5101 aatacggcat ggtcggcatc aatacgggct taatcagcaa tgaagcggca ccgttcggcg
     5161 gcgtgaaacg cagcggtttg ggacgtgaag gcagcaaata cggtgcggac gaatatctag
     5221 aattgaaata tttgtgtatg gatgtcgggt gagggatgcc atccgaacgg cagttcgccg
     5281 gactgtgccg cattttaagc agtctctatc tgttgtacaa tgcgccctgt ttttacggtt
     5341 atttttgatt tgaacaagat atgatggaaa acggaaaaac agtcccgaga tggcgttttg
     5401 ccttgaaaag tgcgggctgg cacctcttaa tcagcctatc ggttgcagga ctggcggcat
     5461 tgctggtttt taaggtttgg tatccttatc cttatgccga gctgacggga gggctgtcgc
     5521 tttatcagct ggtggtggct gtcgatatcg tatgcggccc gctgctgact ttaattttgg
     5581 caagcccgaa gaaaaagaca aaggcacgca tggtcgattt ttccatggtc ggcatcatcc
     5641 agctggcggc tttggcgtac ggtctgcaca gcgtttcgct ggcgcgtcct gtggtggaag
     5701 cgtttgaaca ggatcgtatg accattgtta cggcggcgga agtcgtggtc gaagatttgc
     5761 acaaagcccc cgaagggctg caaagcctgt cgtggttcgg catccgccgc attgcattga
     5821 aagaacctga ggatgcggat gagaagaaca agacgctgga tttgtccctg aaaggtatcg
     5881 agccgagtat gcgtcccgac cggtggctgc cgtattccga caaggaagca gaagaaatcc
     5941 gcaaacatct gaaaccgctg aaagtcttgg cggatgcgag aaaaacgacg gttgcggaca
     6001 ttctgaaaca ggcaggtctc gccgaagggg aggagctgta ttacctgccg tttaccagca
     6061 gcaggcagaa agagtggata gtcattaccg ataaagaggg caacaccaaa ggctacgcgc
     6121 cgatagacgg cttcatcatc accccttaag cgttgggact ccgtccgcac tcgaacatcc
     6181 gttcttcgcg gcggtagaat cagactgtat ttgagagagg cgcaacccct caatcagggc
     6241 aacacatagg ggaaggcttt atgtgccgtc cgtgtgttga aacatttcaa aataagctca
     6301 tgccgtccga acatcctttc agacggtatg gcgttttgcc attgccccga taagctgtta
     6361 aactatttaa attatttcga ccgaaggtac acgcacatgc aagaccccga gcagagcagc
     6421 aagcccgccc gccgtttttt gtgcgtaccg tctgttcagg cagcttgagc gcttcaggct
     6481 gccttttcgc gtttgaactt gaggaatacg aaaatcatgg atttcagttg gttggcagaa
     6541 ccgcatacct ggataggttt tgccacgctt ttggtgttgg aagtcgtatt ggggatagac
     6601 aatcttgtct ttgtggcgat tttggcaaac aaggtccagc ccgcacggcg cgaccgcgca
     6661 cggattatcg ggctggggct ggcagtcgtc atccgcatca ttatgcttgc ttttatggcg
     6721 cacatcatca cgctgaccga gccgctgttc caaatcggcg gcctcgccgt ttccggcaag
     6781 gacatgatta tgctcgcggg cggtattttc ctgctttaca aagccaccac cgaactgcat
     6841 gaacgcctcg aagggcacaa ccgttttacc gttgccgaca gccaaaaaaa acacgcgccg
     6901 ttttggggcg tggtcgcgca aatcctgata ctggatgccg tgttttccat cgattcggtc
     6961 attactgcgg tggcgatggt cgatcatatc gtcgtggcga tgggtgcggt cgtcgtcgca
     7021 atggctgtaa tgatttctgc cagcaaactc ttgaccgaat ttgtcgacag acaccctacc
     7081 gtcgtgatgc tctgccttgg ttttttgttg atgatcggtt tcagcctgat tgccgaagcc
     7141 ttccatttcc acattcccaa aggctacctc tacgccgcca tcggcttctc gattttaatc
     7201 gaactgttta accagatttc ccagcgcaac agccgcaaaa acgactacat cggcagctcg
     7261 tggcgcaaac ggactgctga aaatgtcttg ggtatgatgg gcatacgcga aagcgtgctt
     7321 gccgatgcgg gcggcgaatc tggggacgac gcgcattttg aagaaaacga aaaatcgatg
     7381 atacgcagtg tgctgacgct tgccgaacgc ccgattatgg gggtgatgat cccacgccgc
     7441 gacatcgaac ggctggacat ttcccaaagc cgcgaagaac agtatgccca actgcaaaac
     7501 acgccttaca gccgcctgct cgttgtcgga aaggcgggcg tggacgaacc tttgggctac
     7561 atcaacaaaa aagacctgct gtcccaactg ctggaaacag gcggtctcga cattcagacg
     7621 gcattgcgcc agccgctcgt tttgcccgac agcactaccg cgctgggcgc aatcgaactc
     7681 ttccgccaaa gcagcgcgga ttatgctttg gtggtggacg agttcggcgc ggtattgggc
     7741 atggtaacca tgaaagacct gctcgaaacc atcgcaggcg agttccccga agaatttgag
     7801 cgcgaagaag aaccagccgt tcaggggaat cccgatgaaa gcctgacggt ggaaggcgcg
     7861 ttggaatatg tggaactcgc accgcaactc aacctgccgc agcagcagga agatgccgat
     7921 ttccatacgg ttgccgggct gattatggaa gaattgcaaa ccatccccga tgtcggcgat
     7981 tttgccgatt tccacggctg gcggtttgaa gtggtcgaaa aagaagggca gcgcatcgag
     8041 cgggtcaaaa tcaccaaatt gcccgaagaa taagcattca ggatagaaaa tgaacgtttt
     8101 gatttccaac gacgacggct acctctccga aggtattgcc gttttggcgc gcgttacggc
     8161 ggaatttgcc aacgtcaggg tggttgcgcc cgaacgcgac aggagcgggg tcagcaattc
     8221 gctgacgctg gaacgccctt tgcagttgaa acaggcgcaa aacgggttct actatgtcaa
     8281 cggcacgccg accgactgta tccacatcgg gcagtctgta ttttcggatt ttcaggccga
     8341 ttttgtcttt tcgggcatca accggggcgc gaatatgggg gacgacacgc tttattcggg
     8401 gacggttgcg gcggcaaccg aagcctacct tatgggcata cccgctgtgg cgttttcctt
     8461 aaacgacgct tccggacgct attgggcgac cgcagaacag gcactgtgga cattgttggc
     8521 gcattttttc aaaaaccccc cgcagtcccc tattttgtgg aacatcaata tccccgccgt
     8581 cgagccggaa gatgtgcggg gcattaaaat cgcccgtttg ggcaggaggc atcacggtca
     8641 gaacgtcatc cccgcgcgca atccgcgcgg cgaacagatt tattggatag gcccggtagg
     8701 cagggtttcc gacaaagaag agggaacgga tttcggtgaa tgcggcgcag gtttcattac
     8761 cgtaacgccg ctgcaaatcg acctgaccgc ctatccggac atggcggaaa cagcggtgtt
     8821 ctggcatgcg gactgaccgt ttcat
//
LOCUS       DAWHXZ010000074         8796 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000074 DAWHXZ010000000
VERSION     DAWHXZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.074"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            254..1789
                     /locus_tag="WIE35_001066"
     CDS             254..1789
                     /locus_tag="WIE35_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ4153992.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLNIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     DEK"
     gene            1786..1878
                     /locus_tag="WIE35_001067"
     CDS             1786..1878
                     /locus_tag="WIE35_001067"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ4153993.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1938..3158)
                     /gene="lysA"
                     /locus_tag="WIE35_001068"
     CDS             complement(1938..3158)
                     /gene="lysA"
                     /locus_tag="WIE35_001068"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ4153994.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDTLVA
                     EGIVLEHLDLGGGVGIVYQDESVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIATLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGGGYRLIRRR
                     ETLEQQMANELACL"
     gene            complement(3169..3339)
                     /locus_tag="WIE35_001069"
     CDS             complement(3169..3339)
                     /locus_tag="WIE35_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ4153995.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            3410..3733
                     /gene="cyaY"
                     /locus_tag="WIE35_001070"
     CDS             3410..3733
                     /gene="cyaY"
                     /locus_tag="WIE35_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ4153996.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3760..4776
                     /locus_tag="WIE35_001071"
     CDS             3760..4776
                     /locus_tag="WIE35_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ4153997.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTVLLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVIGGFLVNYGI
                     SKLI"
     gene            4802..5221
                     /locus_tag="WIE35_001072"
     CDS             4802..5221
                     /locus_tag="WIE35_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ4153998.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASKDGILDALHIYNVEDVSDKHIPNHVLILWDDACTLAALCINDYIHAVYDFVAQ
                     AGYCRNGFPETGGEWVKVENRVLDDELLDKILSRKST"
     gene            5243..5770
                     /gene="luxS"
                     /locus_tag="WIE35_001073"
     CDS             5243..5770
                     /gene="luxS"
                     /locus_tag="WIE35_001073"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ4153999.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     TWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNAQSAKNAV"
     gene            5895..8711
                     /gene="polA"
                     /locus_tag="WIE35_001074"
     CDS             5895..8711
                     /gene="polA"
                     /locus_tag="WIE35_001074"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ4154000.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLSAPDGAPTGAMYGVLNM
                     LRRLRADYVHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLMG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKATAPEKLDYQA
                     VTTEAQFAALLDKLSRADTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDAKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QQAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPWDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGIAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNISAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIHNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLSECEASPWDELLQSKLIMQVHDELVLEVVETELDFVKEKLPQIMAK
                     VGGGLLDVPLVAEVGVGENWEEAH"
BASE COUNT         2076 a         2261 c         2310 g         2149 t
ORIGIN      
        1 ctcaacagct taaaatcctg cgggattggt gtgggattta gggctaatct agtacagccc
       61 ctaaaatttt tcgttttcaa accttcaccg cttgccatca gcgttaaatt tttttacgat
      121 aagcacatag attgtaaaca atcggccaca agccggtttg ttttttcaga agacattatc
      181 cctgtcagac gctgtttcta tatatttcgc ctataatggc ttgtttttaa taaataattc
      241 aagaggtatc aacgtgtctg attccaagac gaaagaacgc gccacattcg gcacgcgccg
      301 cgcgtttatg attgccgcca tcgggtccgc cgtcggcttg ggcaatattt ggcgtttccc
      361 ctatattgct tttgaaaacg gcggcggcgc gttcatcctg ccctatctgg tcgcgcttct
      421 gacggcgggc atcccgctgc tgctgctcga ttatgccatc ggccaccgtt accgtggttc
      481 tgcgcccttg gctttccgcc gcctcggacg atggtttgag ccggtcggct ggtggaacgt
      541 gatgaccaat atcgtcatct gcatctatta cgcggtaatt atcggttggg cggcaagcta
      601 tacctattat tcggtcaacg ccgcctgggg tgcggatccg cagggttttt tctttaagga
      661 cttcctgcaa atggcgggcc cggaagcctt gggtttggat tttgtcggca aagtcgccgg
      721 tcctttggcg ggcgtgtggg tttttaccgc cgccattatg gcgttgggcg tgcaaaaggg
      781 cgtggcgcgc gcctcgtcgt tctttatgcc gctgcttttg gtgatgtttt tgattatggt
      841 cggcatttca ctaaccctgc cgggtgcggc aaagggcttg gacgcattgt ttacgcccga
      901 ctggtcgaaa ctcgccgatt ccaaggtctg ggtggcggca tacgggcaga ttttcttttc
      961 gctttccatc tgcttcggca ttatggttac ctattcttct tatttgaaga aaaaaaccga
     1021 cttgggcgga acggggctgg tggtcggttt tgccaacagc agctttgaac tgctcgcggg
     1081 catcggcgtg tttgccgcat tgggctttat ggcgcaggcg ggcggtaagg cggtcaacga
     1141 ggttgcctca ggcggcatcg gtttggcgtt tatcgccttt ccgaccatta tcaaccaggc
     1201 accgatgggc tggctgatcg gcatattgtt tttcggttcg ctggtattcg ccggcgttac
     1261 gtcgatgatt tccatccttg aagtgattgt ggcggcgatt caggacaagc tgaacatcgg
     1321 gcgcgtcaac gccacgctgc tggtctgcat tccgatgggc attgtttcca cgctgctgtt
     1381 cggtacggcg acggggctgc cggttttgga cgtgatggac aaattcgtca acacctacgg
     1441 cattgttgcc gccggctttg tttatgttgc cgccatcatc atcagcggca ggctgccgga
     1501 attacgcaag cacctgaacg ccttgtcctc catccgcatc ggcggcttgt ggacggtctg
     1561 cgtcgtggtt accgtcgtga tgctcggcta tatgctgttt aaagatacca gcggcctgat
     1621 ggagaaaaat tacgaaggtt atccggatgg tttcctcaat attttcggtt gggggatgtc
     1681 ggcggcgttg gtcgtgttcg ggctgctgct gtcgttgctg ccttggaaac acggtcagga
     1741 tttcaacgtc aaagacgaac acgaacatga acaaggagac gaaaaatgag tacttccgcc
     1801 attgtgatga tgattgtctc aatcgtgata atctggggag ggctgctgct ttccctgtta
     1861 aggctgccga acgagtaagc ctttagagcg ttaaaaatgc cgtctgaact gtttcagacg
     1921 gcattttgat gttcggctta caggcaggcg agttcgtttg ccatttgctg ttccaaggtt
     1981 tcgcgccggc ggatgagtcg gtatccgccg ccgtcaacca atacctccgc cgcacggttg
     2041 cgcgcgttgt aattgctcgc catgctggca ccgtatgcgc ccgcgctgcg gataagcagc
     2101 aaatcgcctt cttcgcaggc gatggtgcgg tctttgccga ggaagtcgcc ggtttcacaa
     2161 atcgggccga cgatgttggc ggtcagcgtc gcgatgtttt tggtttcgac cgcttcgatg
     2221 tggtggtagg catcataaag tgccggacgc attaaatcgt tcatcgccgc atcgaccatc
     2281 acaaagtttt tctcttcgcc gtgtttgacg aattcaacgc gcgtcaataa cgcacctgcg
     2341 ttaccgacca agctacgacc tggttcgagg atgagtttca gacggcgtgt gccgatcagt
     2401 ttttgaaccg cttgggcata tgcgcccaaa tcggggacgc tttcgtcttg ataaacgatg
     2461 ccgacaccgc cgcccaagtc caaatgttcc aaaacaatgc cttcggcgac aagagtgtca
     2521 accaaaatca aaatgcgttc gcaggcttcg accagcgggc ttaagtcggt tagttgggag
     2581 ccgatgtggc agtcgatgcc gatgattttc aaattgggct gttgtgcggc atggcggtag
     2641 gcttcgagcg cgtcggcgta ggcgataccg aatttgttgg ctttcaaacc tgtggagatg
     2701 taaggatggg ttttcgcatc gacatcggga ttgacgcgca gggagacggg cgcggtttta
     2761 cccaaacgcg cggcaacttt ctgaatacgg tcgatttcgg ggatgctttc catattgaag
     2821 catttcacgc ctgcattcag cgcgaactcg atttccgcct cgcttttgcc tacgcctgaa
     2881 aatatggttt ttgccgcgtc gccgcctgcc gccaaaacgc gtgccaattc gccgccggac
     2941 acaatgtcaa aaccgctccc caaagaagca aagtgtttga tgatgctcag gttgccattg
     3001 gctttaaccg catagcagac gagcggggag agctcggcaa aggcagtttg gtagtgttca
     3061 aatgcttcgg tcagcgcgga ttggctgtac acataaagcg gtgtgccgaa tgcttcggca
     3121 aggcgggggt aggggacttg ttcgcaaaat agggtcatat tttcgttttc atttttgggt
     3181 tggaggggcg gattgcggtt tgccttgaag ctgcaaaccg gtttggatta cgccgaaacg
     3241 cgccttgtcg ccttctttgg gcaggtagag gtcgcctttg taaccgcagg ccgagagcag
     3301 gagggcggtt gccgccgcaa aaaatacgcc gtatttcatc ggtaaacttc cttcataagc
     3361 gcgaatgtgg caagattcgg catcttaaac aaaaaacacg caaaaagcta tgatgaccga
     3421 aagcgagttt atccgcgcga gcgaagcatt atttgaacac atcgaagacc aaatcgacga
     3481 aaacggctgg gatttcgact gccggtttgc cggaaacgtc ctgaccatcg aagccggaga
     3541 cggcacgcaa atcatcgtca accgccacac gcccaaccaa gaattgtgga ttgccgcaaa
     3601 aagcggcggc taccatttcg ccgaacaaaa cggcaaatgg ctggcaacac gcgacagccg
     3661 cgatttctac gacgttttaa acgaagcact gagcgcggct tcgggcgaag cggtagagat
     3721 tgaggaattg tgatttgggt gttatcacgg agagaaaaaa tgaacacacg tcccttttat
     3781 ttcggactta tatttatcgc gattatcgct atacttgcta actatttagg aaacactgat
     3841 ttttcccatc attatcatat cagtgcttta attattgcta tcttgctggg aatggcaatc
     3901 ggcaatacca tttatccgca attttcaaca caagtggaaa aaggcgtttt gtttgccaaa
     3961 ggcgcgcttc ttcgcactgg cattgtgctg tatggtttcc gcctcacttt tggcgatatt
     4021 gccgatgtag gattaaatgc tgtcgttacc gatgcaatca tgctaatttc aaccttcttt
     4081 tttaccgtac ttttaggcat tcgttatcta aaaatggata aacaattggt ttatctcact
     4141 ggggcaggtt gcagcatttg cggtgcggca gcagtgatgg cggcagagcc tgttaccaaa
     4201 gcagaatccc ataaagtttc agtggcgatt gccgtagtgg tcattttcgg gacgcttgct
     4261 atttttactt accccttgtt ctacacgtgg tcacaacatt taattaacgc ccatcaattc
     4321 ggtatttatg ttggttctag tgtacacgaa gtggctcaag tttatgcgat tggggaaaat
     4381 attgatccta tcgtggcgaa tactgccgtc atttccaaaa tgatccgagt gatgatgctc
     4441 gccccctttt tattaatgct ttcttggtta ttaacacgta gtaatggagt atcagaaaat
     4501 acatcacaca aaattacaat tccttggttt gctgtacttt ttattggtgt tgccattttt
     4561 aattcttttg atttattacc aaaagaactc gtgaaattat tcgttgaaat cgattctttc
     4621 ttattaattt catcaatggc tgcgcttggc ttaacgacgc aagcaagcgc aatcaaaaag
     4681 gcaggattaa aaccgtttgt tttaggaata ctaacttatt tatggctagt gattggtgga
     4741 tttttagtga actatggaat atcaaaatta atataaaatt cactaaagag agcgttaccc
     4801 aatggcacaa ttaccgctat atcggactgc cgaaatcgga aattttactg tcggcacgcc
     4861 taaagtttta gaatcatttt ccaaacatat cccttatggc gtcgtctttg aagacgacgg
     4921 cgacacaggt tacttttatg ccgcctcgaa agatgggatt ttggatgctt tgcatatcta
     4981 caatgtcgaa gatgtatccg acaaacatat ccccaatcat gtcttgatct tatgggacga
     5041 tgcctgcact ttggcagctt tgtgcatcaa cgactacatc catgccgtct atgattttgt
     5101 cgcgcaggca ggatattgcc gcaacggctt ccctgaaaca ggcggcgaat gggtgaaagt
     5161 cgaaaaccgc gtcttggacg atgaattgct ggacaaaatc ttatcccgaa aatctacata
     5221 acctactcaa aaggataaca aaatgcccct gttagacagt ttcaaagtcg accacacccg
     5281 tatgcacgcc cctgccgtac gcgtggcgaa aaccatgacc acgcccaaag gcgataccat
     5341 taccgtattc gacctgcgct tttgcgttcc taacaaagaa atcctgcctg aaaaaggcat
     5401 acacacgctg gagcatttgt tcgcaggctt tatgcgcgac cacttgaacg gcaacggcgt
     5461 ggaaatcatc gacatttccc cgatgggctg ccgcaccggt ttctacatga gccttatcgg
     5521 cacgccttcc gaacagcagg tcgccgatac gtggctggct tcgatgcagg atgttttgaa
     5581 tgtcaaagac caaagcaaaa tccccgagtt gaacgaatac caatgcggca cttatcaaat
     5641 gcactcgctc gccgaagcgc agcaaatcgc gcaaaacgtg ttggcgcgca aagtggcggt
     5701 gaacaaaaat gaagagttga cgctggatga agggctgctg aacgcccaat ccgccaaaaa
     5761 tgccgtctga acaagggttt cagacggcat ttgccttttc cgttataatc cggggttgtg
     5821 cgggggcggg ttttaagccg gcatcgtcct tccctatttt tttctgtccc ttatcggttt
     5881 taagcgggtt ttttatgtcc aacagaccta cactcctcct cgttgacggc tcttcctatc
     5941 tctaccgcgc gtatcacgcg atggggcaga atttgtctgc gcctgacggt gcgccgacgg
     6001 gggcgatgta tggcgtgttg aacatgctgc gtcggttgcg ggcggattat gtgcatgatt
     6061 attgcgcggt ggtttttgat gcgaaaggca aaaatttccg ccatcaaatg tttgaagaat
     6121 acaaggcgac gcgcccgcca atgccggacg atttgcgtcc tcaagcggaa gccttgccgg
     6181 atttggtgcg gttgatgggc tggccggtat tggtgattgg gcaggtggag gcggacgatg
     6241 tgatcggcac gctggcgaaa cagggggcgg aacatggttt gcgagtcatt gtttcgaccg
     6301 gcgataagga catggcgcag ttggtggatg agcgcgttac gctggtgaac acgatgagca
     6361 gcgaaacgct ggacattgaa ggcgtgaagg caaaattcgg cgtgcgcccc gaccaaatcc
     6421 gcgattatct cgcgctgatg ggcgacaagg tggacaacgt gccgggcgtg gaaaaatgcg
     6481 gcccgaaaac ggcggtgaag tggctggaag cctacggctc gttgcaaggc gtgatagaac
     6541 acgcttcgga aatcaagggc aaagtgggcg aaaacctgca agccgcgctg ccccaactgc
     6601 cgctgtcgta tgatttggtc acgattaaaa ccgatgtgga cttgcacgcc gagctttcag
     6661 acggcatcga aagcctgcgc cgtactacgc cgaaatgggc gcagctggtt gtcgatttca
     6721 aacgctgggg cttccgcacc tggctgaaag aagcggaatc aaacatgaat accggctcga
     6781 ccgatgattt gttcggcagc gacagcatcg gcgagcaggc ggctttgaat gcggaaatgc
     6841 cgtttgaaaa acaagccgaa aaagccaccg cccccgaaaa actggattat caagccgtta
     6901 ccaccgaagc gcagtttgcc gctttgttgg acaaactgtc gcgggcggac acaatcggca
     6961 tcgatacgga aaccacgtca ttagacgcga tgaacgcctc gctggtcggc atcagcatcg
     7021 ctttccaagc aggcgaagcg gtttacatcc ccgtaggaca cagcctgacc gccgcgcccg
     7081 aacagcttga tttgcaagac gtattaggcc gtctgaaacc gcatttggaa aaccccgccc
     7141 tgaagaaaat cgggcaaaac ctcaaatacg accaacacgt tttcgctaac tacggcatcg
     7201 ccctgaacgg cattgccggc gacgctatgc tcgcttccta catcatcgaa agccatcttg
     7261 gacacggctt ggacgaattg tccgaacgtt ggctgggctt ggaaaccatt acctacgaat
     7321 cattgtgcgg taaaggcgcg aagcaaatca gttttgccga tgtcgccatc gggcaggcga
     7381 ccgaatacgc cgcccaagac gccgatttcg ccttgcgcct cgaagcgcac ctgcgcgcgc
     7441 aaatggacgc caaacagctt gaaatgtatg aaaaaatgga gctgcccgtc gcgcaagtat
     7501 tgtttgaaat ggaacgcaac ggtgtgcaaa tcgaccgcgc cgaactcgcc cgccaaagcg
     7561 cggaactcgg cgccgagctg atgaagctcg aacaacaagc ctacgccgcc gcaggccagc
     7621 cgttcaacct caattcgccc aaacagctgc aagaaatcct gttcgacaaa atgggcatcc
     7681 cgacaaaagg gctgaaaaaa actgccaaag gcggcatttc caccaacgaa gccgtgctcg
     7741 aacagctcgc gcccgactac cccctgccca aaatcatcct gcaaaaccgc agcctggcga
     7801 aactcaaatc cacctacacc gacaaactgc ccgaaatgat ttctccctgg gataaccgcg
     7861 tgcataccac ctacgcccaa gccgtcgcca ttacaggacg cttggcaagc aacaacccca
     7921 acctgcaaaa catccccatc cgcaccgccg aaggccgccg cgtacgtcgc gcctttaccg
     7981 caccgcaagg cagcgtcatc gtttccgccg actattccca aatcgagctg cgcatcatgg
     8041 cgcacctctc tggcgacaaa accctcatcg ctgcgttcca aaacggcgaa gacgtacacc
     8101 gccgcaccgc cgccgaagtg ttcggcatcg cccccgaaaa cgtctcgtcc gaacaacgcc
     8161 gctacgccaa aaccatcaac ttcggcctca tttacggcat gggccaatac ggcctcgcca
     8221 aatcattggg catcgacaac atctccgcca aaaactttat cgaccgctac ttcgcccgct
     8281 atcccggcgt cgccgaatac atgcagcgca ccaaagaaca agccgccgca caaggctttg
     8341 tcgaaaccct gttcggcaga aggctctacc tgcccgacat ccacaacaaa aacgccaacg
     8401 cccgcgccgg agccgaacgc gccgccatca acgcccccat gcaaggcacc gcctccgacc
     8461 tcatcaaacg cgccatgata gacgtgtccc gctggctttc agagtgcgaa gcctccccgt
     8521 gggacgaact cttacaaagc aaactgatta tgcaggtgca tgacgaactg gtgctggaag
     8581 tcgttgaaac cgaactggat tttgtcaaag aaaaactgcc gcagattatg gcgaaagtgg
     8641 gcggcggatt attggatgta ccgctggtgg ctgaggttgg cgtaggggag aattgggaag
     8701 aggcacattg attgaaaggt gttatatgct atctttattt aaataaaatt taatttttgg
     8761 tatatttttt ctaaatgttc ctatagtata gtggat
//
LOCUS       DAWHXZ010000075         8597 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000075 DAWHXZ010000000
VERSION     DAWHXZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.075"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..282)
                     /locus_tag="WIE35_001075"
     CDS             complement(<1..282)
                     /locus_tag="WIE35_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ4154001.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKSTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYFAERQNHINGIENFWNRAKRHLRKF"
     gene            complement(588..1847)
                     /gene="rho"
                     /locus_tag="WIE35_001076"
     CDS             complement(588..1847)
                     /gene="rho"
                     /locus_tag="WIE35_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ4154002.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDSINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(2067..4451)
                     /gene="ppsA"
                     /locus_tag="WIE35_001077"
     CDS             complement(2067..4451)
                     /gene="ppsA"
                     /locus_tag="WIE35_001077"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ4154003.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPMLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFAINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4863..5684
                     /locus_tag="WIE35_001078"
     CDS             4863..5684
                     /locus_tag="WIE35_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ4154004.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(5923..6585)
                     /locus_tag="WIE35_001079"
     CDS             complement(5923..6585)
                     /locus_tag="WIE35_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ4154005.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTLLARHGLPPKSMDEIRNQ
                     ASHGAAGLLKLGAGITPDHTDYARWRTEYLDEYDSRYAQDTTLFGGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(6646..7611)
                     /locus_tag="WIE35_001080"
     CDS             complement(6646..7611)
                     /locus_tag="WIE35_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ4154006.1"
                     /translation="MSHHHEHHHHDHSHAHTHTANKKVLRVSFIIIAAFMLLEAFGGW
                     LTHSLALLSDAGHMFSDAFSLGVALWAFKLGEKETTLQKTFGYKRFEILTAMFNGLSL
                     VVIAVLIFYEAVKRLLYPPEIATLGMLVISVVGLLVNIGVAVYMLKNSDTEANVNMRG
                     AYLHVLSDLVGSVGAIAAAVLMMAFGWQWADPLASVFVAALVGRSGWSLLKQTLHILM
                     EGAPENIHTDDLLAVIRNTDGVKSVHDLHVWTITSNINVLSCHIVVDGSMTVAESEQI
                     AYRIEHELSHKNIGHCTIQIESERHPHPDSVLCANETDGAHHHHH"
     gene            complement(7657..8484)
                     /locus_tag="WIE35_001081"
     CDS             complement(7657..8484)
                     /locus_tag="WIE35_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ4154007.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNMDGAETNTPP
                     LDFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRERLRAFGFDYGDFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQSKKVV"
BASE COUNT         1806 a         2060 c         2527 g         2204 t
ORIGIN      
        1 aaacttgcgt aaatgacgtt ttgcccgatt ccaaaagttc tcaattccat taatatggtt
       61 ttgtcgttca gcaaaataac tttcatctgc ttctacttcg ccatcaaaca tttccaaatg
      121 tggactgttt tgataaataa gtaatcgtaa acgatgaaaa taataggctg cggtactttt
      181 attaacgcct actaactctg ctgctgttct tgcagttaca cctgcgacaa acagttcaat
      241 gagtttattt tgtttatacc ggcttagacg actttttctc ataggggcaa ctctaactta
      301 atttgaattt ccctagttat ctaggacagc cccaaattta tacaaaaatg agcgcggttc
      361 ggcgcaacct tgaatcaagt tcccgcatcg gttttcattg ccggtacgga tgcgttcaag
      421 ccggttttgc aaaggccgcg ctttcggcaa gcggacacgg acactgccga cggttgcgcc
      481 gttaacgggg ggagggagga gctgcgccga ccgtgtgaat gaaagtgccg tctgaaaccc
      541 gattttcagg cttcagacgg catttcgcat taatgcgggc ggcgcgttta tttgccgcgc
      601 atcagttcaa agaaatcgtc gttgttttta gaggctttga ttttcccgtt taagaactcg
      661 gtagcttcga tttcatccat cgggtgcagg aacttgcgta agagccacat acgttgtaac
      721 tggtcgttcg gcacaagcag ctcttcgcgg cgcgtgccgg atttgttgat gttgatggcg
      781 gggaagaggc gtttttccgc catacggcgg tcgaggtgca attccatatt gccggtgcct
      841 ttgaattctt cgtaaatcac atcgtccata cggctgccgg tttcaaccaa tgcggtggca
      901 atgatggtca gcgaaccgcc ttcttccacg ttgcgcgctg cgccgaagaa acgtttgggg
      961 cggtgcagcg cgttggcatc gacaccgccg gtcaggattt tgcccgaggt ggggacgacg
     1021 gtattgtagg cgcgggcaag gcgggtaatc gaatccagca ggatgaccac gtcttttttg
     1081 tgttctacca tacgcttggc tttttcaagc accatttcgg caacttggac gtggcgttgc
     1141 gccggttcgt caaaggtgga ggaaaccact tcgccgcgga cggaacggct catttcggtt
     1201 acttcttcgg gacgctcgtc aatcaagagg acgatgagct cgacttcagg atagtttgcg
     1261 gtaacggcgt gggcaatatt ttgcaacatc acggttttac cgcttttggg cggggcaacc
     1321 aagagggcgc gctgaccttt gccgatgggg gaaatcagat cgatggctcg tccggtcagg
     1381 ttttcttcgg actttaagtc gcgttccagc ttcaactgtt cggtcggaaa cagcggggtc
     1441 aggttttcaa acaggatttt atggcggcat acttccgggt ggtcgccgtt gatggaatcc
     1501 agtctgacta gggcaaaata gcgttcgttg tctttgggga cgcgcacgct gccttcgatg
     1561 gtgtcgcccg tatgcaggtt gaagcggcgg atttgggtgg gcgagacata gatgtcgtcg
     1621 gggcccgcaa gataggacgt gtccgcgctg cggaggaagc cgaagccgtc gggcaggatt
     1681 tcgagcgtgc cggagcaggt gaaaccctcg ccttttttca tcatttggcg gacgatggca
     1741 aatacgaggt cttgtttgcg gaatcggttg gcgttttcga tgccgtgttc ttccgccaat
     1801 tctaagagtt tggaaatgtg cagggtttgt aattcggaga cgtgcataat aatgatgtat
     1861 tttgaagagg aaaaagacag gcagatgccg tctgaaagaa gaagctgacc gttgccggtt
     1921 gctcggggaa gggggaattg taggcagtcg gcgcgtgggt gtcaaatatt atcgcggacg
     1981 gggcatcggc aggaaatgcc gtctgagcgg agctgcttgg aaaaaaatac ccccgcgctt
     2041 ttcaggctcg ggggtatggg cattgattat ttgttcaatt cattcgccaa atatagccaa
     2101 gtttcgatga cggtatccgg gttcagggaa acgctttcga taccttcctc aaccagccat
     2161 ttggcgaagt ccggatggtc ggacgggcct tgaccgcaga tgccgacata tttgttctgc
     2221 ttgcggcagg cggagatggc aaggtgcagc atcactttga cggcagggtt gcgttcgtca
     2281 aacgattcgg ataccaagcc gctgtcgcgg tcgagaccga gggtcagttg ggtcatgtcg
     2341 ttcgagccga tggagaagcc gtcgaagtat tgcaggaatt gttccgccaa taccgcgttg
     2401 ctcggcagct cgcacatcat aatcaggcgc aggccgtttt tgccgcgttc caagccgttt
     2461 tctttcaggg ctttgacaac ggcttcggct tcgcccaaag tgcggacgaa cggaatcatg
     2521 atttcaacgt tggtcaaccc catttcatcg cggacgcgtt tcaaggcttt gcattccaag
     2581 gcgaaacagt ctttgaagtt gtcggcgaca taacgcgccg caccacggaa gcccaacatc
     2641 gggttttctt catgcggttc gtatacgctg ccgccgacca ggttggcgta ttcgttggat
     2701 ttgaagtcgg acatacggac gatggtttta cgcggataaa ccgatgcggc caatgtcgcc
     2761 acgccttcgg cgattttatc gacgtagaag tcgacagggg acgcgtaacc ggcgatacgg
     2821 cgggtaattt ccgcttttaa ttcgtcatct tgtttgtcaa attccaacaa ggctttcggg
     2881 tggataccga tttggcggtt gatgataaat tccatacgcg ccaagccgat gccttcgctg
     2941 ggcaggttgg cgaagctgaa tgcgagttcg gggttgccga cgttcatcat gactttgacg
     3001 ggtgctttgg gcatattgtc caaggcgaca tcggtaattt gtacgtccaa cagaccggca
     3061 tagatgaagc cggtatcgcc ttcagcacag gatacggtaa cttcttgacc gtttttcagc
     3121 aattcggttg cattgccgca gccgacaacg gcaggaatgc ccagttcgcg cgcgataatc
     3181 gccgcgtggc aggtgcgtcc gccacggttg gtaacgatgg cggaagcccg tttcatcacg
     3241 ggttcccaat ccggatcggt catgtcggta accagtacgt cgccggcttc gacggaatcc
     3301 atctcggaag catctttaat caggcgtact ttgccctgtc cgactttctg accgatggca
     3361 cggccttcgc ataatacggt tttttcgccg ttgatggcga agcggcgcag gttgcggctg
     3421 ccttcttcct gggatttgac ggtttcggga cgggcttgca ggatgtagag tttgccgtcc
     3481 aagccgtcgc gtccccattc gatgtccatc gggcggccgt agtgtttttc gatggtcagt
     3541 gcgtaatgcg ccaactcagt gatttcttcg tcggtaatgg agaagcggtt gcggtcttcc
     3601 tcggggacat cgacgttggt tacggattta ccggcttctg ctttgtcggt aaaaatcatt
     3661 ttgatgtgtt tcgaacccat ggtcttgcgc aggatggcgg gcttgcccgc tttgagcata
     3721 ggtttgaaca cataaaattc gtccgggttg accgcacctt gtacgacgtt ttcgcccaga
     3781 ccgtaagagg aggtaacaaa gacgacttga tcgtagccgg attcggtgtc gagggtgaac
     3841 atcacacctg atgcgccgct gtcggaacgc accatgcgtt gaacgccggc ggaaagggcg
     3901 acgatgtcgt gttcgaagcc tttgtggacg cggtaggaaa tggcgcggtc gttgtacagg
     3961 gaggcgaata catggtgcat cgcttcttta acgttttcca atccgttgat gttcaagaag
     4021 gtttcctgtt ggccggcaaa tgatgcgtcg ggcaggtctt cggcggttgc ggaagagcgt
     4081 acggcaacgg aaatgtccgc accgccggca tcggcaacca ttttgttcca tgccgcttcg
     4141 atttcggcat cgagctgttc ggggaagggc gtgtccaaaa tccattggcg gatttctttg
     4201 ccgacgcgtg ccagttcggc aacgtcttcg acatctaatt ttgccagtgc ggcggaaatg
     4261 cgttcgctca gaccgttgtg tgcgaggaat gcgcggtagg catcggctgt ggtggcaaag
     4321 ccgccgggga cgcgaacgcc tttttcggtc agctgactga tcatttcgcc cagcgaggcg
     4381 tttttaccgc ccacgcgttc aacatctgtc atacgcaggt tttcaaacca gattacgtag
     4441 ttgtcggcca tttgtgtgtc caatccaaaa tatgttaaaa aagaaacaaa tccgcgtgct
     4501 tattttaagc gattcgttcc tctgctgtca tgtgttttat ccgttttaaa atcatgatgc
     4561 cgtctgaaaa attgcggttt cggcgtgtgt agcggtttga aacttgcagc cggtatgttt
     4621 ctttttttgg gtattttctt tgtaaaacag atggtttgaa taggttaatg ttttttctgt
     4681 ttgatttttt gtttattttt ttaaaatttt atgccaaaaa acactttatc taaataattt
     4741 tatttcaaaa taatattgtg tctgtttggg tgtaatccga ggtaggtgtg ctgcgggtgc
     4801 tttccttgtg tctgctgctg ctgttatgat gagattttaa acctgtgttt taaggatgga
     4861 agatgagcag tccgcgccat gtgttttaca tttccgaccg taccggtctg actgccgaga
     4921 atatcggcga ggctttgctg aaccagtttg gcaatctgtc gttcaaacgc catacgcatc
     4981 cgtttgtcga tacgccggaa aaggcgcgcg cggtggtgga gaaggtcaat cggagccggc
     5041 aggaaaacgg tcagcgtccg attgcgtttg tcagtgtggt tgatgacgaa atccgtcgga
     5101 ttatcaaagg ggcggatgct tttcagatta atttctttga gacttttttg ggactgttgg
     5161 agaaagaact caataccgaa gccacggcat ccgggcaggg gcatcacagt atcggtaata
     5221 cgaagcgtta cgatgcacgt atggaggcgg tgaatttctc attgaaccac gatgacgggg
     5281 tcagcgataa gaaccttcag gaagcggatg taatcttgat gggtgtatcg cgttcgggca
     5341 aaacgccgac ctgcctttac ctcgccctgc aatacggcat ccgtgcggca aactatccgc
     5401 tgattcccga cgatttggaa tcggccgatc tgccgcgtat ggtcaagcct tatagggata
     5461 agctgttcgg gttgaccatc cagccggaac gtttgcaggc catccgccaa gagcgccgcc
     5521 cgaattcaac ttatgccaaa atcgatacat gccgcagcga ggtggcggac gcgcagagta
     5581 tgttcagacg gcatggcatt ccgtttgcga atacgacgga taaatcagtg gaagagcttg
     5641 ccgtacacat ccttcaggcg tgcaagctca aacgcaggtt ttgacgggct ttgattcggt
     5701 ttgaaggtgg aaatgccgtc tgaaatcagg tttcagacgg cagttttata gtggattaac
     5761 aaaaatcagg acaaggcgac gaagccgcag acagtacaaa tagtacggaa ccgattcact
     5821 tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgaggca acgctgtact
     5881 ggtttttgtt aatccactat atgtttgtgg ggcggatatt tttcagggct gtattttgtc
     5941 cagacattcg agcagatcga gtggcgtgcg gatgtggaaa tccgcctgcc atgagccggt
     6001 atcgtcttcg ggagcgatgt agccccattc ggcgaggacg gttttcatgc cggcgttgcg
     6061 gcctgcttgg atgtcgcgtt cggcatcacc gacgtagagt gtgtgttgcg ggtcggcgtg
     6121 gatttgtccg cacgcataca gcatgggttt gatgctgggc ttgggttcgc cgcaggtgtc
     6181 gccgctgacg acgacggcgg gtgggatgat gaagccgagt ttggggacga gtttgtcggt
     6241 gaagcgcata ggtttgttgg tgatgatgcc ccatttgatg ccgcgtctgt cgagttcggc
     6301 gatgagttcg tttacgccgc cgaagagggt ggtgtcttgg gcgtagcggc tgtcgtattc
     6361 gtcaaggtat tcggtgcgcc atcgggcata gtcggtgtgg tcgggggtga tgcctgcgcc
     6421 gagtttgagc agacctgccg cgccgtggct ggcttggttg cggatttcgt ccatgctttt
     6481 gggcggcagt ccgtggcggg cgagcagggt attgagtgcg ccgccgaggt ctagggcggt
     6541 gtcggcgagc gtgccatcga ggtcgaacaa tacggcttgt atcatgtgtt ttcctttggt
     6601 ttttcgatgg ggtcgtctga aaagggtttc agacgacctc tgggtttagt ggtggtgatg
     6661 gtgtgcgccg tcggtttcgt tggcacataa gacgctgtcg ggatgcgggt ggcgttcgct
     6721 ttcgatttgg atggtgcagt gtccgatgtt tttgtgggac aactcgtgtt cgatgcggta
     6781 ggcgatttgt tcggattcgg caacggtcat gctgccgtcc acgacgatgt ggcaggataa
     6841 tacgttgata ttgctggtaa tcgtccaaac gtgtaagtcg tggacggatt tgacgccgtc
     6901 ggtgttgcgg ataacggcga gcaggtcgtc ggtgtggatg ttttcgggtg cgccttccat
     6961 caggatgtgc agcgtttgtt tgagcaggct ccagccgctg cgtccgacca acgcggcaac
     7021 gaacacgctg gcgagcgggt ccgcccattg ccagccgaac gccatcatga gtacggcggc
     7081 ggcaatcgcg ccgaccgagc cgacaaggtc gcttaaaacg tgcagatacg cgccgcgcat
     7141 gttgacgttg gcttcggtgt cgctgttttt cagcatataa accgccacgc caatgttgac
     7201 cagcagcccg acgacgctaa tcacgagcat gcccagcgta gcgatttcgg gcggataaag
     7261 caggcgtttg acggcttcgt agaaaatcag cacggcgatg accaccagcg acaagccgtt
     7321 gaacatggcg gtgaggattt cgaaacgttt gtagccgaag gtcttttgca gcgtggtttc
     7381 tttttcgccg agtttgaacg cccacaacgc cacgcccaag gaaaatgcgt cgctgaacat
     7441 atgccccgcg tcggaaagca gcgcgagcga gtgggtcagc cagccgccga acgcttccag
     7501 cagcataaag gcggcgatga tgatgaacga cacgcgcaat acttttttgt tggcggtatg
     7561 cgtgtgggcg tgggaatgat cgtgatgatg gtgttcgtgg tggtgagaca taatgttcct
     7621 caacagaggt cgtctgaaaa tccggattcg gggttttcag acgacctttt tgctttgttt
     7681 caaaccctgc tcgaaatctt ccaacatatc caattcgaag cggctgaagc ccgctttttc
     7741 gcgcgcttcg atgttcacat agccgcggaa gataaacata tcgtaacggg caatcagact
     7801 gcggaacagg gcgacaggtt ccaaaccgcg ttcgcggcag aggtgttgat accaatggtt
     7861 gccgatggcg acatgaccca cttcgtcgcg gtaaatgatg tccaatacgc cgcaggtttc
     7921 cgaatcgccg cgctgcgcca ccttcgcgcg tatccccggc gtaacgtcca gcccgcgcgc
     7981 ttccaacacg cgcggcacta aagccatacg caacaaagga tcgtaggcgg ttttgtatgc
     8041 catatcccat aaatgattgt gcgcctcgaa atcgccgtaa tcgaagccga aagcgcgcag
     8101 cctttcgcgc atcaggcgga agtggtacac ctcttccttc gccactttca cccagtcgcg
     8161 gacaaactga aacggcagcg tgcggaaacg gtatgccgcg tccaaagcca gattgacggc
     8221 gttgaattcg atatgcgcaa tcgcgtgcag catcgccgca tagccttctg ccgtgttcat
     8281 tttgcgcggg gtcagttgcg acggcgcgac caaaacaggc ttgtccggcc gccctgcgcg
     8341 ggggaaatcc agcggcggcg tgtttgtttc agcaccgtcc atgttctgaa cggcggcaaa
     8401 cgcctcatcc gtcagccgtc ctttttcatc tgggtcgccc gaaagcaggg cgcgttccag
     8461 caaagcataa atatcaagtt tcatctcaag tccgccgtgt tcggaaaaca aatattatag
     8521 cgttttaaaa aaacaagatg aggcatataa tctccgcgat tcggcattcc gcgcccaaac
     8581 cgtcaaatat agtggat
//
LOCUS       DAWHXZ010000076         8435 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000076 DAWHXZ010000000
VERSION     DAWHXZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.076"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..861)
                     /locus_tag="WIE35_001082"
     CDS             complement(<1..861)
                     /locus_tag="WIE35_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ4154008.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFN"
     gene            1066..1344
                     /locus_tag="WIE35_001083"
     CDS             1066..1344
                     /locus_tag="WIE35_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154009.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSDSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(1400..2017)
                     /gene="recR"
                     /locus_tag="WIE35_001084"
     CDS             complement(1400..2017)
                     /gene="recR"
                     /locus_tag="WIE35_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ4154010.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(2084..3622)
                     /locus_tag="WIE35_001085"
     CDS             complement(2084..3622)
                     /locus_tag="WIE35_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ4154011.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKVKPANGKPAYVRLTGLP
                     APVIVEVQAVTPPDDAAARIPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(3682..4056)
                     /locus_tag="WIE35_001086"
     CDS             complement(3682..4056)
                     /locus_tag="WIE35_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ4154012.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQVPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKALFSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            4206..5834
                     /locus_tag="WIE35_001087"
     CDS             4206..5834
                     /locus_tag="WIE35_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ4154013.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            5906..7159
                     /locus_tag="WIE35_001088"
     CDS             5906..7159
                     /locus_tag="WIE35_001088"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ4154014.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWFALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKTFRAAQDKTEKH"
     gene            7382..7690
                     /locus_tag="WIE35_001089"
     CDS             7382..7690
                     /locus_tag="WIE35_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154015.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            7772..>8435
                     /gene="ruvB"
                     /locus_tag="WIE35_001090"
     CDS             7772..>8435
                     /gene="ruvB"
                     /locus_tag="WIE35_001090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ4154016.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEI"
BASE COUNT         1993 a         2416 c         2161 g         1865 t
ORIGIN      
        1 gttgaacgcc gccacggcga taatcagcgt caggatgatg aacatcatcc gtttttccaa
       61 ctcgaccgcc tcaaaatagc ttcgattgct gaacgtccaa tcgcgcaccc aaaccgtatc
      121 cctttgcgcc tccggaatca gtgttgccgt caaggcgggg gcgttttgcg ggtcggcgag
      181 tttcagccgc agccccgcaa cttccttatc caagcggtac agtacgcgcg cgtcttggat
      241 atgcgtcatc gccaatgagt tgtccacttc gtaaacgccc gtcttaacca gaccgaccac
      301 ggtaaactgc ttcaggcgcg gcacgactcc ggcaggggta acgttgccct ccggcgtgat
      361 gacggtaact ttattgccga cttccgcccc caaagcctcc gccaagccga caccgaggat
      421 aatgtcaaac tcgcccggaa tcaggtcttc aaatttgcct gccggcattt tgtcgccgta
      481 ttccaccact ttgcgctctt cagacggcaa aatgccgcgc atctgcacgc ccctgatttc
      541 gcccgcattg gccagcaatg cctgattgga aacatagggc gcggcagcca aaataccttt
      601 gcggttttcg gtaaaccgaa gcaggttgcg ccaatccgta tccgtattgt cgatatagcc
      661 gatttcggcg tgcggcgcga cattcaggag ctgcccgcgt atttctttct gaaagccgtt
      721 cataaccgac aagacgacaa tcagcgcggt tacgcccaag gcgattccgg caatcgaaac
      781 catcgtgata aacgacataa agccgttgcg ctttttcgcc ctgagatacc tcaagcctat
      841 ccaagcctct agagaaaaca taacgctacc ttaaaaatgt ctgcaaacgt gccgccccgg
      901 acggcggttt gggggcggcg gcaaaagttt attgtaccgt aaaaccccgg cagcgtccga
      961 acgcccgagt gcgggggacg gggcggcaga gcgggcggaa aacttgcaca acgcgtcaaa
     1021 ctgccctatc ctttccttca gaaaaaccgt ttcttgagga aaacaatgaa tatccgaact
     1081 gcttttgctt tgtgcgccat cgccttatcc gccgctgcgg ctgcctacgc caaagaaatc
     1141 aaaatcgatg ccaacaacac gccttattcc gaagccgacg cgcaaaagct ggcggcaacg
     1201 gcagtcggta tgggtgttaa ggaacctatc agcctgaacg gcggcagcga cagcattacc
     1261 gtgtccggca gcagcgcgac gcagtgcgtg ttcaaagtcg gcaacggagg cgcattgcag
     1321 attcaagggc taaactgcaa gtaaaccgcc cggaaaaatg ccgtctgaag gcttcagacg
     1381 gcattttgca ttggcggcgt tatgccccgc cttctttaat caggcggcgt tcgtacaccg
     1441 cctgcgccag cgttcccgca tcgacatatt ccaattcgcc gcccaaggga atgccctgcg
     1501 acagcctgct gactttgtaa ggcaggtttt taaaaaactc ggacaggaca tacgccgtcg
     1561 cattgccttc tgcggtaaaa gcggttgcaa taatgatttc ttcgacttcc ccgccgccca
     1621 gccgttgcgc cagcctgtcc aatgcgatgg cggatacgtc cattcccaat gccgtattga
     1681 tttgccccat caggacgaaa tacagcccgt cgtggcagtt tgccgcttcc atattcgaca
     1741 cgtcggcagg catatgcacc accatcagcc gccgcccgtc gcgtgtttca tcggcacaaa
     1801 tatcgcacaa tccgccttcg caaaacgtgt tgcacatcgc gcaatggtaa acctgcttca
     1861 atgccgtctg caaggcatcc accagttttt cagcctcttt gcgcttgtgt tggagcaaat
     1921 gatacgctat ccgctgtgcc gatttcggcc cgacgttggg taaaaccttc agcgcgtcga
     1981 tcaatccttg gaaggcatct tgttttttgt ggctcatcat attccgccgt atgggaaaac
     2041 ggccggaata ttccgaccgt tatgttgtca acaaaagtgt caattactga ccgtcgccgt
     2101 tgtcgaccga ttgcgctcct ttggtctgtt tgattttgcc gttgaaataa cgtatcaaca
     2161 agtcgaaggt attggcagac tgctgctgcg ccaaagcctg ttttgcaagc gggatgcgcg
     2221 cggcggcatc atccggcggg gttacagcct gtacttcgac aatcacgggt gccggcaggc
     2281 cggtcagcct gacataggcg ggtttgccgt ttgccggttt tactttcagc agttccgcat
     2341 aagcctcggg cggcatggac tgccttgcct gctgtgcgcc caaaacggac acttccgacc
     2401 atttcacgtc aactgccttg ccgccgttca gttgggtaag cacgtctttt gccttgtttt
     2461 cggcaagttt ggcggcttcg gtacggatat aagcctgacg taccgcgtct ttggcttcgg
     2521 caaacggcag ggttttctct tcgcggactt ctttggcgcg gacgacccac gcggtttcgc
     2581 tgttgatggt cagcacttcg gaattgtgtt ttttcttcaa tacgtcgtcg ctgaatacgg
     2641 cattgatcag gttttcgggc ataccggaca tttgcgcatc ctgcctgctc agccaagttt
     2701 cttgggtttc gactttcaaa ccgctgtttt tggcggcttc ggcaagcgag gaaggatggt
     2761 tgaacgcgtc atcgcccagc ttttcttttg ccttattgaa gtcggcaacc gcctttttca
     2821 ttttcaattc gttttctacg gcggcttttt cctgctcgaa agaaggtttg gcttcatttg
     2881 ccggcaaacg cgccacgcgc tcttcaaacg catttttcac ttctgtttcg ctgacggtct
     2941 gtttgtctgc aaagtctttc agattcaagg cgacatattc caatttgacc gctttgggaa
     3001 gcaggtagtc ttttttgttt gcgttataaa acttctgcaa atcggcttca gacactttga
     3061 cttgggcgat gaattcgtcg gggttgaaag tgtgcgaacg gatggtgcgg ttgacctgcg
     3121 tcagcctgat cagctgttcc gcctgcgcgt cgccgaccaa tacgccgttt tggacgaggt
     3181 ttaccaaatt ctgcaaggca aactgatcgc ggatttcttc gacaaactgg tcttcagaca
     3241 tatggcgttg cgacaggtag cggtttaaaa gcgcgtggtc gaatttgccg tttgcgtcgt
     3301 ggaaattggg atcatccacg ataatctgct tgatttgttc ggaagaaacc gaaatgccca
     3361 tcagcttcgc gccctgtttc aggtaggcgc gttgcagcag ggattggaac accgcgtcgc
     3421 gcgaagggcc gccgccgtcc gcctgttcgt tctgtatggc gttgttgatg gagtggtcgc
     3481 tgattttttc gtcgcccact tggacgatgt agtcggcacc cggatgggat accgtgctga
     3541 ccccgaagcc gacgaaggtt aatgcaatca ggcccaaaag gacttgggcg ggcgttctgt
     3601 atttttcgat ggaatggaac atattttaaa tcgggatata gaatgggaac gggaaattca
     3661 agtcgggtat tgtaacggtt tttatccctg tctgcacggg gcttgccggt tgaagatgcc
     3721 gtcgtaggtt tcttcgctga agccggcgta aaccttgccg ccgcactcca atacgggacg
     3781 cttgatcagg ctcggcattt cggacatcag tttgacggcc tccgctgtcg agaacagggc
     3841 tttttgctgt gtttcggcat cgagtttgcg ccagcttgtc ccgcgtttgt tgagcagggt
     3901 tgccaaaggc acttgttcca gccacgagca gatttccgct tcggaaggca cctgtttttt
     3961 aaaatcccga aattcaaact ccaagccgta tccggcaagc cggtttttgg cttttttgac
     4021 cgtatcgcaa tttgggatgc cgtgaaggac tatcatttgg aaaccttttg tctgaaataa
     4081 taaaacggat attttactat aagtgtctga aaatttgccc gtctgtttca gacggcaggg
     4141 cggttatgtt acaatccgaa aattcgaaaa atttaatctc ttgttcaata aaggctttac
     4201 caatcatgat ttctaccaac ggcatcacca tgcagttcgg cgcgaagccg ctgtttgaaa
     4261 acgtatccgt taaattcggc gaaggcaacc gctacggttt gatcggcgcg aacggctcag
     4321 gcaaatccac cttcatgaaa atcctcggcg gcgatttgga acagacggcc ggcgaagtgg
     4381 cgattgaaaa cggcgtgcgt ttgggtaaat tgcgccaaga ccagtttgcc tacgaagaca
     4441 tgcgcgtgct ggacgtggtg atgatggggc ataccgaaat gtgggcggcg atgaccgaac
     4501 gtgatgcgat ttacgccaat cccgaagcca ccgaagacga ctacatgaaa gccgccgaac
     4561 tggaagccaa gttcgccgaa tacgacggct acaccgccga agcgcgtgcc gccgaactgt
     4621 tgagcggcgt gggcatttcc gaagatttgc acaatgcgaa aatggcggaa gtcgccccgg
     4681 gcttcaaact gcgcgtattg ctggcgcagg cgctgttctc caagccggat gtattgctct
     4741 tggacgaacc gaccaataac ttggacatta ataccatccg ctggttggaa ggcgtgttga
     4801 accaatacga ctccacgatg attatcatca gccacgaccg ccactttttg aacgaagtct
     4861 gcactcacat ggcggatttg gactacaaca ccatcaccat ctatccgggc aactacgacg
     4921 actacatgct cgcctccgcc caatcgcgcg aacgcgccct gaaagacaat gccaaagcca
     4981 aagagaaact gcaagagctg caagagttcg tcgcccgctt ctctgccaac aaatccaaag
     5041 cccgtcaggc aaccagccgt ctgaaacagg ccgacaaaat caaatcggag atggtcgaag
     5101 tcaaaccttc tacccgtcaa aacccgtata tccgttttga agccgatgaa aaagccaagt
     5161 tgcaccgtca ggctgtggaa gtggaaaaac tggcgaaacg ctttgaaacc cagttgttta
     5221 aaaacctgaa cttcatcctt gaagcgggtc agcgtctcgc catcatcggt ccgaacggcg
     5281 cgggcaaatc taccctgctg aaactcttgg cgggcgcgta caaccccgaa tattcagacg
     5341 gcctgctgcc ggacgaaggc agcatcaaat gggcggaaaa agccagcgtc ggctactatc
     5401 cgcaagacca tgaaaacgac ttcgacgtcg atatggatct gagcgaatgg atgcgccaat
     5461 gggggcagga aggcgacgac gaacaagtca tccgaggcac tttggggcgt ttgctcttcg
     5521 gcagtaacga tgtcgtgaaa aaagtgaagg ttctctccgg cggagaaaaa ggccgtatgc
     5581 tttacggcaa actgttgctg ttgaaaccca atgtcttggt catggacgaa ccgaccaacc
     5641 acatggatat ggaaagtatc gaatccttga acatggcgct ggaaaaatac aacggcacgc
     5701 tgattttcgt ctcgcacgac cgtcagttcg tttcctcctt ggcaacccaa atcatcgaac
     5761 tggacggcaa aggcggatat gaacactact tgggcgatta cgaaagttac ttggagaaaa
     5821 aaggcgtagc ataaccgccg gttggaacaa tgccgtctga agccgcttca gacggcattg
     5881 ttgataactt taaaatagga agcatatgca gacttacctc gtcggcggcg ccgtccgcga
     5941 ttatcttttg ggcttgcccg tcaaagaccg cgattgggtg gtcgtcggcg cagacgcaca
     6001 aaccatgctg gcgcaaggct tccagccggt cggcaaagat tttcccgtgt ttctccatcc
     6061 cgaaacgcac gaagaatacg ccctcgcccg caccgagcgc aaaaccgcca aaggttacgt
     6121 cggtttcagt ttccacgccg acaaagacgt tacgctggag caggacttga tgcgccgcga
     6181 cctgaccatc aacgcgatgg cgcaagatgc ggacggcaag attatcgacc ctttcggcgg
     6241 acaacgggat ttggcggcag gcattttgcg ccacgtttcc ccggccttcg ccgaagaccc
     6301 cgtccgcatc ctgcgtactg cccgctttgc cgcgcgttac aggtttgaaa tcgccgaaga
     6361 aaccataaag ctgatgcggc agatggtgga aaacggcgaa gcggacgcat tggttgccga
     6421 acgcgtctgg caggagtttg cgaaaggttt gatggaaaaa aatccgcgca aaatgattga
     6481 agtgttgcgc gaatgcggcg cgctcaaagt cttgctgccc gaagtcaatg ccctcttcgg
     6541 cgtgccgcaa cgcgccgact accatcccga aatcgacagc ggcatccata ccctgatgac
     6601 gctgcaacgc gccgccgata tgggtttgag cctgcccgaa cgctatgccg ccctgctgca
     6661 cgacttgggc aaagccaaaa caccgtccga catcctgccg cgccaccacg gacacgacct
     6721 cgccggtgtc gaacccgtgc gcgaagtcaa tcagcggctg cgtgcgccga aacattgcgc
     6781 cgagcttgcc gaattggttt gccgttggca cattattttc caccaagtcg gacagcttaa
     6841 aagccaaacc attctgaacg tattgaaaaa aaccgatgcc ttccgccgtc ccgaacgctt
     6901 tcagacggca ttgaacgtct gcattgccga cacgcaaggc cgtctgaacc gcgaacacac
     6961 gccctacccg caacgcgcac actggttcgc cctgctcgaa gccgccaatc aggcggattc
     7021 gggcaaaatc gccgccgaat gccgcgcgca gggaaaagcg caccttatcg ccgaacaaat
     7081 cgaccgggcg cggctggcac aaatcgcccc attgcaaaaa acgtttcgag cggcgcaaga
     7141 caaaacagaa aaacattaaa acgtccaatg cagccacttt tatagtggat taacaaaaat
     7201 caggacaagg cgacgaagcc gcagacagta cagatagtac ggcaaggcga ggcaacgctg
     7261 tactggtttt tgttaatcca ctataaagtt ttgaggacga tacccaatcc aagctttgca
     7321 acagccgccg ccatatccgc tataattcac gcttcagcca tcctgccccc gacataaaat
     7381 catgaccctg aaaaccgatt tattgcctaa aatcaacaac gaagattatc aacgcctcat
     7441 cctcaaacac agtgcggaat ttagcggtgg cgaaatccgc ctgttgaacg aaatcctcga
     7501 aaaattcaat ttcgacgttg ttcaggcgca ggcattggcg caagccgtca tgcagcaaat
     7561 ccgcttcgac cccaacgcct accacatcga cagcgacgac gaagacacca ccggcatctg
     7621 cccccactgc atcaacccgc ctatgccgcc cctgcgcgac tatctcgttt ggcgcgaaac
     7681 ccgcggataa aacgcttttg accgttatct tttcaatgcc gtctgaaacg ccgccgaccg
     7741 ttcggacggc atacccgaca aagggaacac tatgctgcaa accgacaacc tgaccgccgc
     7801 gcaaccgcaa cgcatcgttg ccgcccaaac cgcctccgca caggaagaac tgctcgaacg
     7861 cgccctccgc cccaaaacgc tggacgacta catcgggcaa gacaaagcca aagaacagct
     7921 tgccattttc atccaagccg ccaaaaaacg cggcgaagca ctcgaccacg ttttgctctt
     7981 cggcccgccc ggactgggca aaaccacact ggcgcacatc atcgccaaag aattgggcgt
     8041 aaatttgcgc caaaccagcg gccccgtcct cgaacgcgca ggcgacctcg ccgccctttt
     8101 gaccaacctt gatccgcacg atgtattgtt catcgacgaa atccaccgcc tcagccctgt
     8161 tgtcgaagaa atcctctatc ccgcgctcga agactaccgg ctcgacatta tgataggcga
     8221 aggacccgcc gcccgttccg tcaaaatcga cctgccgccc ttcacgctca tcggcgcgac
     8281 cacccgcgcc ggtatgctga ccaatccgtt gcgcgaccgc ttcggcatcg tctcccgcct
     8341 tgagttttac gaaaaccgag accttaccac catcgtcagc cgttcggcac aactgttgca
     8401 gctcgatatg tccgaagaag gcgcggaaga aatcg
//
LOCUS       DAWHXZ010000077         8366 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000077 DAWHXZ010000000
VERSION     DAWHXZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.077"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1..363)
                     /locus_tag="WIE35_001091"
                     /pseudo
     CDS             complement(1..363)
                     /locus_tag="WIE35_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            410..979
                     /locus_tag="WIE35_001092"
                     /pseudo
     CDS             410..979
                     /locus_tag="WIE35_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            1211..1711
                     /locus_tag="WIE35_001093"
     CDS             1211..1711
                     /locus_tag="WIE35_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154017.1"
                     /translation="MVFVNPLYYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAAL
                     FITLFFAAVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIERLARLQQPDLPEK
                     AVRYTRRVTQVWCVFFIANGTLAALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYR
                     KLVLKV"
     gene            1718..2233
                     /locus_tag="WIE35_001094"
     CDS             1718..2233
                     /locus_tag="WIE35_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ4154018.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENAVYF
                     GMICNRLPSYESDTFGLHCNVVTQPDGQRPLLQLHQSGGHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            2253..3731
                     /gene="ubiD"
                     /locus_tag="WIE35_001095"
     CDS             2253..3731
                     /gene="ubiD"
                     /locus_tag="WIE35_001095"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ4154019.1"
                     /translation="MKYKDLRDFIVMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     SALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTSYP
                     VSVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDYFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVDNTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWGELGL"
     gene            complement(3796..5910)
                     /locus_tag="WIE35_001096"
     CDS             complement(3796..5910)
                     /locus_tag="WIE35_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ4154020.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAIGLHASVYRILTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDRTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTIGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            6001..6894
                     /locus_tag="WIE35_001097"
     CDS             6001..6894
                     /locus_tag="WIE35_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ4154021.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            7034..7888
                     /gene="folP"
                     /locus_tag="WIE35_001098"
     CDS             7034..7888
                     /gene="folP"
                     /locus_tag="WIE35_001098"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ4154022.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNARTA
                     LAHAEQLLKEGADILDIGGESMRPGAADYVSPEEEWARVAPVLAEVAGWGVPISLDTR
                     RTVIMEKALALGVVDIINDVAALTDKGAVELLARQADTGICLMHMQGLPENMQINPKY
                     QDVVGEVVRYLKERAAECIAAGIAPQRITLDPGFGFGKNLQHNIALMRHLPELMAETG
                     FPLLIGVSRKSMIGELTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALKA
                     WEALGINL"
     gene            8018..>8366
                     /gene="glmM"
                     /locus_tag="WIE35_001099"
     CDS             8018..>8366
                     /gene="glmM"
                     /locus_tag="WIE35_001099"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ4154023.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFF"
BASE COUNT         1804 a         2054 c         2539 g         1969 t
ORIGIN      
        1 tctttttgat gacagggagt aacgtttcag acttggcatt gtttacgaca acggtataga
       61 cccgtccgtt gcgtttcaga atgccgaaga caaccacttt tcctgccgcc cgcgaccacg
      121 tctgccttta cgccgtccgc cgaaatcgct ttcgtccggc tcgacaggat cctcaaaaac
      181 ctcatcggca gccaagacca aatgatggtt gataaccgtg cggattttac ggtagaacag
      241 tgctgccgaa ttgggatgga tacccaaaat atcggcggca gaacgggcgg taacttccag
      301 tacaaaaaaa tggagcagtt ctttctgtac ttttttcttt aatttgcagt gtgttatctt
      361 catatttcga gggtaacata tctgctaatc tagtaaaatt taatccacta tagtttatgc
      421 ctcgcacgac ggggaaatgg cgcgcagttt cgatttgtgg ttggaactgt tgaaatcgca
      481 aatcgtgtcg ccgacttcgt tcggtttgtc ggtgcacaat gcaacggcgg ggtaatggtc
      541 gatattgagg cgggatatga gcgagcagac ggcattggcc gtgtgtgccg acggtgtgga
      601 aacggcattg gcggaagcgg catcactgtt ggaagaggga tgcgggtcgg ttttggtttt
      661 ggcggcggac gatccgctgc cggaaggata tgccgtatcg gcaacgcgcg cgccgtttgc
      721 ttatgctttg gcgatggttc tgaccaaagg gacgcgttac agcctgactt tatctgcttc
      781 ggacgatatg ccgtctgaag ccggtatgct gcccgaggca tattggagcg gtttggaatg
      841 ggtgcgcttt ctgctgaacg gcagtcggga atgccgccgg gtatatcgca atcgtgaatg
      901 gctgtggcag cccgcaagct gcaggctgaa gatttccgca atgtccgcac cgtcaacgac
      961 gtggtacagg cggtattgaa gattcaggcg ggctaatcgg gtatttctaa ttatttttca
     1021 gacggcatgt ttttatcgga tgccgtctga aaacatttgt gcggattcaa aatgaaaccc
     1081 ggttttttcg gacatatttt tctgaccgta ttaagcattg cctatcccgc tttgtggtat
     1141 agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacgg caaggcgagg
     1201 caacgctgta ctggtttttg ttaatccact atattacggc agggaggcgg gctggtttgt
     1261 ctggctggcg gcggcgatgt gcgggttgtg gctgctgcgc gccctgactg ccaaactgcc
     1321 ccaacagcgt tatgcggctt tgtttattac actgtttttt gccgcagtat tggtgttcgg
     1381 gcggcatgat tcgatgtatt ggtatcccgt cctcgtcaat ctgatgatgt tggcggtgtt
     1441 cggcggcagc ctgtttgccg gacagacggt catagaaagg ctcgcgcggc tgcaacagcc
     1501 cgacctgcct gaaaaagctg tgcgctatac ccgccgggta acgcaggttt ggtgcgtatt
     1561 ctttattgcc aacgggacgc ttgccgcttt gttggcccgg ttggggcgat atgattggtg
     1621 ggcggtttat acaggcgtga ttgcctatgt attgatgggg atgctgtttg ccggcgaatg
     1681 gctttaccgc aaactcgttt tgaaagtctg aaaccttatg tacacttgca cttcctgcgg
     1741 cgaaaaacat gaagatatgc ccgcaatcgg ctttaccgcc cccgatccct ataatcaact
     1801 cagtgaagaa gaacgcattg cttatcaagc agaatgcgat tctgatttct gcatcatccg
     1861 ctatcccgac caaaccgacc gctttatccg cgcggtgctg ccgattccga ttatcgggca
     1921 tgaggaaacg ctggaatatg gcgtgtgggt gtccgtcagc gaaaagagtt ttaacgacta
     1981 tcaaagccac tttcacgaca atccggaaaa cgccgtttat ttcggcatga tctgcaaccg
     2041 gctgccctct tatgaatccg acaccttcgg tttgcattgc aatgtcgtta cccaacccga
     2101 tggtcaacgc cctttgctgc aacttcatca aagcggcgga catccgctgg tacgcgactt
     2161 ttatcacggc atagaatatg ccgaagcaca ggcaaggata ggggcggtat ccgaatcgga
     2221 ttgttcagga taaaaggaca aaaaggataa atatgaaata caaagacctg cgcgacttca
     2281 tcgtcatgct cgagcagcaa ggcaaactca agcgcgtcgc ccaccccgtt tccccgcatt
     2341 tggaaatgac cgaaatcgcc gaccgcgtgc tgcgtgccga agggtcggcg ttgttgtttg
     2401 aaaacccgat taagcccgac ggtacgcgct acgattatcc cgtgttggca aacctgttcg
     2461 gcacgcccga acgtgtggcg atgggcatgg gcgcggacag cgtgtccaag ctgcgtgaaa
     2521 tcgggcagac gctggcgtat ttgaaagaac ccgaaccgcc caaaggcatc aaagatgcgt
     2581 tttccaaact gccgctactg aaagacattt ggagcatggc gccgaacgtg gtgaaaaacg
     2641 cgccgtgtca ggaaatcgtg tgggaaggcg aaaacgttga tttgtataaa cttccgattc
     2701 agcattgctg gccggaagac gttgcgccgc tggtaacatg gggcttgacc gtaacgcgcg
     2761 ggccgcacaa aaaacgccaa aatctcggca tttaccgcca acaactcatc ggcaaaaaca
     2821 agctgattat gcgctggctg tcgcatcgcg gcggcgcgtt ggattatcag gaattccgca
     2881 aactcaatcc tgatacgtcg tatcccgtct ccgttgtgct cggctgcgac cccgccacca
     2941 ttttgggcgc ggtaacgcct gttcccgata ccttgagcga ataccagttt gccggactgc
     3001 tgcgcggttc gcggacggaa ctggtgaaat gtatcggcaa cgatttgcaa gtgcccgcac
     3061 gtgccgaaat cgtactggaa ggcgtcattc atccgaacga aaccgcgttg gaaggcccat
     3121 acggcgacca cacgggctat tacaacgagc aggattattt ccctgtgttt acggtcgaac
     3181 gcattaccat gcgcgaaaac ccgatttacc attcgaccta cacgggcaaa ccgcctgatg
     3241 agcctgccgt tttgggcgtg gcgttgaacg aagtgttcgt accgcttttg caaaagcagt
     3301 tccccgaaat caccgatttc tacctgccgc ccgaaggctg ctcctaccgc atggcggtgg
     3361 tgagcatgaa aaaacagtac gccggacacg ccaagcgcgt gatgatgggc tgctggtcgt
     3421 tcctgcgcca gtttatgtat accaaattca tcatcgtggt ggatgacgat gtgaacgtgc
     3481 gcgactggaa agaagtcatc tgggcggtaa ccacgcgcat ggaccccgtg cgcgacaccg
     3541 ttttggtgga caacacgccc atcgactacc tcgacttcgc cagccccgtc agcggactcg
     3601 gcggcaaaat gggtttggat gcgaccaaca aatggccggg agaaaccgac cgcgaatggg
     3661 ggcgcgtcat caaaaaagac cctgcggtta cggcgaagat tgatgggatt tggggggaat
     3721 tgggcttgta ggttttacgg caatgccgtc tgaagcgcga gggcgtttca gacggcattt
     3781 ttgtacgcag gatgtttacg gttctttagg tttgagccac tgccattgtt ccagcagggt
     3841 gtcttcgagg attttctgtt tctctttgcg cgaattgatg ccgccggtca gcctgccgta
     3901 atcgacggta atggacggct tgtccaccgt accggtgatt ttcaggggaa tcggtttgtt
     3961 tttcggatgg acggcgttgc ggataaggac atcttcagac aattcctgcg tatccagatt
     4021 ggtatagccg ttgctggtaa catagaggct gtcggagaag agttcggtat cgatgtggcg
     4081 gctgatgccg tctgaaattt cgctgttgag cgtgaatcgg tggaagggtg tgctgggtgc
     4141 ggcattgtcg gcagttttgc ccgaaatgcc gtttttcagg atattgtcca tgtcgatacc
     4201 gtgccatgca ccgttggaaa tatttagcga caggctgccc tgaagcgagc ggataagctc
     4261 ttttcgggtt tcgccgcccg cggtcaggtc gatgaccgcg tcgccgttgc cgctgaagct
     4321 gtggaagccg aacaggtctt gcagcagcgg ttggatttgg atgttgcttg cattctgttg
     4381 caggcggtaa gtggcgggac gggtgttggc gatgctgatg ccgccttcgg tatggccgcc
     4441 gtaaagccct gacttgaaac ggctgagcgc gatatggtct ttgtcggcgt ggaggtaggt
     4501 ttccatatcg tccagttgca ggccgggaag ttggactttt ccgattttca ggtgcgcctc
     4561 gatgtcgccg gacagcttgg cgagggtgtc gggaaatatt ttgccgtttt gttgccgcac
     4621 gtcgtcaaga tagggggtca ggttcaattt ttgcagtgcg acggcggctt ccagatgcgg
     4681 tgcgtcttca tgtgtgtatc tgaatttcgc ggcaacggtt tggcggtcga atgtgccgtt
     4741 taattcggca ttccaatttt gcagattcgg tacggacagc gaaccgtcga gccggctgat
     4801 gaaacggggt tgcggcaggc ggttgacggt atcctgaagg gtcgatacat acaggcgcgg
     4861 cgcgtccagc cctttgtttt ccgtccatac gagcggcgaa ttgagggaga agttggtctg
     4921 gtggcgcggt gttttgaagc tgccggagat ttcggcgttg ccgatgttgg cgatgccgga
     4981 gtgcaggttg gctttgtcga gtttgaacga accgtcccat cgggcatatt cgccgccgat
     5041 ggtaaatgcg ccgttgacgg tttcaatttt aatgctgttg ttcctgagtg ccagcgtagg
     5101 gatttgggcg gtcaggtgga ggttgcggaa ggaagtgtct gcacgcaggc cgaggccggc
     5161 ggcatccgct ccgccggcgt tgaaacggac agaagggctg ccggtggtgg aaatggtgat
     5221 gccgtgtccg tccagcgaag tggaagcttc aaaatggaac ggtgagattt tgggcgtgcc
     5281 gatgccgtct gaaaggaaca gccccctgct tttccacggg acggacagct ttccccaaac
     5341 cagtatgccc gaactttcaa acggctgccc cgacgaatcg ggggattgca ggttgaggtt
     5401 gatttccttc aggataagct gttcctgcag gaaattgagg cggacggtgc tgttttcgac
     5461 gataatgcgg ttgactgagg cttggcgttt ttggctgtcg atcaggtctt ggatgttcca
     5521 aacacctttc ccgtcgcgcg tcagggcaag ttccgcactc gaaaccaccc atttttcaat
     5581 ctgtatctga tccgaccaca ggtttttcca gctcaatccg attttggttt cttggacgga
     5641 aacggcagtc cggtcgccgc cgggttcggt aatggtcagg tttttcagga tgacggtcgg
     5701 ccggggcagg agcctgcgct gaatgtccgc atcaaacgag attttccggt gcgtatgagc
     5761 aatgctttgt tggaggcggc ttctgatgtt ttcaggtgtg aggatgcgat atacggaagc
     5821 gtgcagcccg attgccgcca aaagcagtat cgtcagcacg gccaccgcat atttcagacg
     5881 gtatttgtgg aaaaccgata ataaatccat agcccgaaca atctgtaaaa cacgccacag
     5941 tataacacgg cttgcaaaaa tcctattttg ggcaggccgt ttcttgtgtc aggaggaaat
     6001 atgattattg tcatgagcag gcgtgccgcc gaagcggaca ttgccggcgt tgtcgccttt
     6061 atccgcagca gaggtctgcg agaacacatt tctcacggcg acgagcgtac tgtcatcggc
     6121 gcaatcggcg acgatagggt tttgagcgtc agggaggtgc agacgctgcc ggaggtcgag
     6181 aaagccgtcc gcattttgga tacatggaaa accgtcagtc gggaaaatcg ggcggaagac
     6241 agccgcgttg ccgccaaagg cgtggctttc ggcggcgggg aaaccgtccg tatcgccgca
     6301 gaaccgtcag tatggcgcaa tgcggatgca gtgtttctgg atccgttttt cacttcggca
     6361 aacttatacg acacttcaag tgcggatgaa gggcggggac ggtgcagacg gttggcggaa
     6421 cagaccgcat cggcgcatgg cgcagggaaa cccgtattgg tgcgcgtccg caacgtccgc
     6481 catgtcgaag cggctttgaa tgcgggggcg gacatcctgt atttgggcgg cgggctgatg
     6541 tccgatcttg ccgtattgaa tgaagccgga agcctgaaca ttcccttggt gctgtgtaaa
     6601 gacaagcacc attctgccga agattggttg aatgccgcag aatatgtcgt ctcgcgcggc
     6661 aaccgccacc tgattctggg cgagtcgggc gtgttgagcc ataccaaaga ccatccctac
     6721 cgtttggatg tcgaatccat cgtgaaagtc cgccaaatca gccatctgcc cgtgattgcg
     6781 aatattacgg gattatggaa ccgcgatatg ccgcaggaaa tcctgtacgg cttggcaaag
     6841 gcggcaggcg cgtgcggaat cgtcggaacc tgttttgaaa agtcgggcgg atagcaggca
     6901 taccggcaga ttcaaaggcg gagcagacat tcggacaaca tgccgtctgc cgcccgaata
     6961 gcggtgccgt ctgaagcgca caagacggtt tcagacggca tataatctgt tttttatttt
     7021 gggaggagtt cagatggcac gacacgtttg gcaggcagga cggtttgaaa tcggtttgga
     7081 caagccgaaa atcatgggca tcgtgaatct cacgcccgat tctttttccg acggcggcgc
     7141 gtattcgcaa aacgcccgaa cagccttggc acacgccgaa cagcttttaa aagaaggtgc
     7201 agacattctc gacatcggtg gcgaatcgat gcggccgggt gcggcggatt atgtttcgcc
     7261 ggaagaagaa tgggcgcggg ttgcgcctgt gttggcggaa gtagcagggt ggggtgttcc
     7321 catcagtttg gacacgcgcc gtacggtgat tatggaaaaa gcgttggcac tcggtgtcgt
     7381 cgatattatt aatgatgtgg cggcgttgac tgacaagggc gcggtcgaat tgctggcgcg
     7441 tcaggcggac acgggcattt gcctgatgca catgcagggt ttgcccgaaa acatgcagat
     7501 taatcccaaa tatcaagatg tggtcggcga agtggtgcgg tatttgaaag agagggcggc
     7561 ggaatgtatc gcggcaggca tcgctccgca acgcatcacg cttgatccgg gtttcggttt
     7621 cggcaaaaac ctgcaacaca atatcgcgct gatgcggcac ttgcccgaat tgatggcaga
     7681 aacgggtttc ccgctgctga tcggtgtgtc gcgcaaaagc atgataggcg agctgaccgg
     7741 cgaggcggac gcagcggcgc gcgtacacgg cagcgtggcg gcggcgctgg cttccgtagc
     7801 gcgtggcgcg caaatcgtgc gggtgcatga tgtgaaggcg acggcggatg cgttgaaggc
     7861 gtgggaagcg ttgggcatca atctgtaaac agcggttcgc atacttcatg ccgtctgaaa
     7921 aggacggggc ggcttcagac ggcatcaacg gtgcagacag cagggcacaa acagactgta
     7981 taatctcgga caacggttca cattctcgga gcaacacatg gcaaaaaaat atttcggcac
     8041 ggacggcgtg cgcggcgaag tcgggcagtt tccgattacg cccgattttg tattgaaact
     8101 cggctatgcg gcggggcagg ttttggtgca gcacgacacc gaccaaaaac cgaccgtcct
     8161 catcggcaaa gacacgcgca tttccggcta tatgctggaa gccgctttgg tggccggttt
     8221 tacggcggcg ggtgtgaatg tggtacagac cggaccgctg cccacgcccg gcgtggctta
     8281 tctgacccgc gcgttgcgcc tgtccgccgg cgtgatgatt tccgcgtcgc acaacgccta
     8341 ttccgacaac ggcatcaaat tcttcg
//
LOCUS       DAWHXZ010000078         8325 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000078 DAWHXZ010000000
VERSION     DAWHXZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.078"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(151..1083)
                     /gene="cysK"
                     /locus_tag="WIE35_001100"
     CDS             complement(151..1083)
                     /gene="cysK"
                     /locus_tag="WIE35_001100"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ4154024.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1277..1882)
                     /locus_tag="WIE35_001101"
     CDS             complement(1277..1882)
                     /locus_tag="WIE35_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154025.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1879..2730)
                     /gene="dapF"
                     /locus_tag="WIE35_001102"
     CDS             complement(1879..2730)
                     /gene="dapF"
                     /locus_tag="WIE35_001102"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ4154026.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFIPEHAPIAAWADRFRGIG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     AKGIIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAQVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            2868..3164
                     /locus_tag="WIE35_001103"
     CDS             2868..3164
                     /locus_tag="WIE35_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154027.1"
                     /translation="MFRILLLLFPCAILVACTRNIPANPTVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3178..3846
                     /locus_tag="WIE35_001104"
     CDS             3178..3846
                     /locus_tag="WIE35_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ4154028.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3919..6039)
                     /gene="pnp"
                     /locus_tag="WIE35_001105"
     CDS             complement(3919..6039)
                     /gene="pnp"
                     /locus_tag="WIE35_001105"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ4154029.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYINGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQAAINA
                     INEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWNA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RVGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6276..7139)
                     /locus_tag="WIE35_001106"
     CDS             complement(6276..7139)
                     /locus_tag="WIE35_001106"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ4154030.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKVLEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYEVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7338..8213
                     /gene="rfbD"
                     /locus_tag="WIE35_001107"
     CDS             7338..8213
                     /gene="rfbD"
                     /locus_tag="WIE35_001107"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ4154031.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLLSSGY"
BASE COUNT         2020 a         2380 c         1924 g         2001 t
ORIGIN      
        1 aaatatttcc ctcaagcaat gcggcggcac caatgcctga ttaaaacaca accggcgttt
       61 tcggcatata tgcaaaaacg cattttccgg cacggcgtta caccgatacc gaaaaatgcg
      121 tctgaatgtt tcgacaatcc gattaaagca ttatgccaaa tctgcaaaaa gtggcgtaga
      181 gagatagcgt tcgccataag aaggcagcag cacgactatc agcttgcctt cgttttcagg
      241 ctgtttggca agctgcaacg cactccaaac cgccgcaccg gaagaaatac ccaccaaaat
      301 gccttctttt tccgctattg cgcgggcggt ttcaaaagcc gcttcgttcg acactttggt
      361 aatgctgtcg taaattttgg tattcaaaac ggtcggaata aagcctgcgc cgatgccttg
      421 aatcgggtgc gggccttttt cgccgccgct caatacgggt gaagcttcag gctcgacggc
      481 aaccacttta acttcgggtt tgtatttttt caacacttcg cccacgccgg taatcgtacc
      541 gcccgtgccg acgccggcaa cgaagacatc gactttgccg tccgtatccc gccaaatttc
      601 ctcggcggtt gttttgcggt ggacttcggg gtttgcctca ttgtcgaact ggcgcggcat
      661 aaaataagtg tcgggatgcg cgtccaccaa ggatttcgct ttggcaatcg cgcccgccat
      721 accttcggcg gcaggggtca gaatcagctc cgcaccaaac gcgcgcaaca gcattttgcg
      781 ctccttactc atgctttccg gcatggtaat cgccagcttg tagccgcgtg cggcacatac
      841 cattgccaaa ccgacacccg tattgccgct ggttgcttcg acaatgacgg tgtttttgtt
      901 gattttgccc gctttttcgg caccctcgat cattgcttcg gcaatgcggt ctttgacgct
      961 gctgcctgga ttgaaaaatt ccagtttcac ggcaacctcc gccttcaaac cttcggtcag
     1021 acggttcagt ttgaccaaag gcgtgttgcc gattagttcg gtgatgctgt ttgcaatttt
     1081 catcttctgc tcctgaataa taaggtttcg acagtaaggc gcaatcccca taggcatcct
     1141 gtccgattgc aggggatttg cgccgaaaca taaacaaatt tcggatatgg ctttatccta
     1201 aacctatgcc gcctaaaaaa ccaatatcca aaatttcttt ctttataact ttttgttccg
     1261 atacggccgc accgtattat cttccaattt ctgaaagcag cagcaccagt accgagacct
     1321 gcatcaacgc tgccgtcaac ccgccgagtc gcagctgcga gccgccgaac gttgacggcg
     1381 gctgccggtc tataccgcag cggtggacat aggcaaccgc catcagcaca aactgcaaca
     1441 cgaaccaata aaccggcttc agcgcataaa gtttgagcag tgccgcaaac acaaacactt
     1501 gaaccgttcc cgaaaagcaa taatgcacca acgcacatat tccgacaaaa gcaagcgtca
     1561 tcgatacatt actgacggca aaaagcccga gcagcggatg ttcggggtct gcctgccatc
     1621 cgtttccatc cgttttccgg ttccaatgcc cgatgaaaaa aaatatgctg cccaaagtca
     1681 ggtaaaaatg ggggatgaac aagggcgcgg cgcgggtcat tccccatatg ccgggcatca
     1741 gctttgcccc caaaaccgat atgcccagcc acagcataat actcgcccaa aaccattgca
     1801 acattcctgc gcggaatatc agaaacagaa aaaccgccgc atacagtaag gcagacaaaa
     1861 aatccaaatg cagcaaaatc atgaatacgc caactcgcct tcaaacaccg tttccgcagg
     1921 gccggtcatc atcacatcgc cgccgcaggc ccattcgata tataaagttc cgcccggcaa
     1981 aatcacctct accgttttcc cttcatccaa caaccccaga cggatacccg ccaccacagc
     2041 cgcacacgcg cccgtaccgc acgcttgggt ttcgcccacg ccgcgctcga acacgcgcaa
     2101 acgaatcgcg gttcggctga caatctgcat aaagccgacg ttgacgcgtt cgggaaactg
     2161 cctgtgcggt tcgataagcg aaccggtttc gcgcacctgc gcgcattcta cgtcatcgac
     2221 cacaatcacc gcatgggggt tgcccatatt gacgcagctg acaggctgaa tgccggattc
     2281 gagatgcacc ccgtaaatac aggcatcatc cccctcgccc gattcgggga caaacggtat
     2341 ttcagacggc ataaacttcg gtttgcccat attgaccgta accatgccgt tatcggacaa
     2401 tttcggaaaa ataatgccct tggccgtttc aacacaaatt tctttcttat cggtcaaacc
     2461 cttgtctgca acaaaacggg caaaacaacg cgctccgttg ccgcattgcc cgacctcgct
     2521 gccgtcggca ttgaaaatac ggtaacggaa atccacacct tcggtttccg aacgcccgac
     2581 caccaaaagc tggtcgaacc cgatgccgcg gaaacggtca gcccatgctg caatcggtgc
     2641 atgctcagga ataaaatcct gactgaccgc gtcaatcacc ataaaatcgt tgcccaaacc
     2701 gtgcatttta gtaaatttca aagtgttcat attattctga aatcccgtat tatctgttgc
     2761 ttccgaattc cgaaatcccc cgcaccgaag ggaaatgctt ccatctcccg ccgcctgagg
     2821 attaaaatca gccattatac tgtttgttac cccttacaag aaacaccatg ttccgcatat
     2881 tactgttgct tttcccttgt gcaatccttg tcgcctgcac ccgaaacata ccggcaaacc
     2941 cgaccgtttc aaattgcgcg catcttcaaa accgcaccat cctgtatttt gacgcacacg
     3001 gcaaacccgc cgacggcgca gtaaagggcg gattttacaa aattatcgaa atacagggtg
     3061 caaactgcat ccttattcaa gactttaaca gcacgggcaa tgccctcggc ggatcctatg
     3121 tgccggactg ccgcaaatca tcccttttcc cgaaaaatat ttgacttcaa actgcctatg
     3181 aaccacgaca tcactttcct caccctgttc ctgctcggct tcttcggcgg aacgcactgc
     3241 atcggcatgt gcggcggatt aagcagcgcg tttgcgctcc aactcccccc gcatatcaac
     3301 cgcttttggc tgatcctgct gcttaacaca ggacgggtaa gcagctatac ggcaatcggc
     3361 ctgatactcg gattaatcgg acaggtcggc gtttcactcg accaaacccg cgtcctgcag
     3421 aatattttat acacggccgc caacctcctg ctgctctttt taggcttata cttgagcggt
     3481 atttcttcct tggcggcaaa aatcgagaaa atcggcaaac cgatatggcg gaacctgaac
     3541 ccgatactca accggctgtt acccataaaa tccatacccg cctgccttgc ggtcggaata
     3601 ttatggggct ggctgccgtg cggactggtt tacagcgcgt cgctttacgc gctgggaagc
     3661 ggtagtgcgg caacgggcgg gttatatatg cttgcctttg cactgggtac gctgcccaat
     3721 cttttggcaa tcggcatttt ttccctacaa ctgaaaaaaa tcatgcaaaa ccgatatatc
     3781 cgcctgtgta cgggattatc cgtatcatta tgggcattat ggaaacttgc cgtcctgtgg
     3841 ctgtaagccc cattcattcc cgatacccgt aaaaaatacc gtctgaaacc tgttcagacg
     3901 gcactttcac cctaagcgtt actcggcggc attttcctca cgggcaggcg cgtccagcag
     3961 ggctttgatg gacaggcgca cacggcctct gtcatccact tccaatgctt tcacgttcac
     4021 cacctgaccg acttgcaggt agtcaccgac attgcgtacg cgctcgtggg cgatttggct
     4081 gatgtgtacc aaaccgtctt tgcccggcat cacgctgaca atcgcgccga cgttgttgtc
     4141 gaggattttc accacagtgc cttcatacac tttgcccact tccacttcgg cagtaatctg
     4201 ctcgatacgt tttttcgccg catcgccggc ttcttgagtg gtcgcagcaa tggtaatcgt
     4261 accgtcttcg gcaatattga tttccgtacc ggtttcagcg gtaatcgaac ggatggtttc
     4321 gccgccctta ccgataactt cgcggatttt gtcttggttg attttcatcg taaacaaacg
     4381 cggcgcgtgt gcggacaact cttgcgggcc cgcaacggcg gctttcatct gatccaagat
     4441 gtgcagacgc gcttctttgg cctgtgccaa agcgatttgc ataatttctt tggtaatgcc
     4501 ttggattttg atgtccattt gcagcgcggt aacgccttcg gtcgtaccgg ccactttaaa
     4561 gtccatatcg cccaagtggt cttcgtcgcc caaaatgtcg gtcaggacgg caaatttgtt
     4621 gccttccaga atcagaccca tcgcgatacc ggcaacgtgt gctttcaaag gcacgccggc
     4681 agacagcagg ctcaggcagc cgccgcagac ggaagccata gaggaagagc cgttggattc
     4741 ggtaatttcg gagaccacgc gcatggtgta gctgaaatct tcaggtttcg gcaatacggc
     4801 caacaatgca cgtttagcca aacggccgtg accgatttca cggcgtttcg gcgcacctac
     4861 gcgacccact tctccggtag agtacggcgg aaagttgtag tgcagcatga agcggtcggt
     4921 gtattcgccg gacagcgcgt caatgatttg ctcgtcgcgc gaagtaccca aagttgcaac
     4981 cgccaaagct tgggtttcgc cacgggtaaa caatgcggaa ccgtgtgtac gcggcaatac
     5041 gccggtttgg atgttcaaag gacggacggt gcgggtgtcg cggccgtcga tgcgcggttg
     5101 accgtccaaa atttggctgc ggacaacatc ggcttccaag tgtttgaaaa tacctttgat
     5161 ttcgttggcg gccaaagtat cggtttcttc ggtaatcaag gcttctttta ccgcattcca
     5221 agcctcgtcc aatttggcgg aacgcgcttg tttttgacgg attttgaacg cttctttaat
     5281 ggtttcgccg gcaatcccgc ggactttggc aactaattct tcattggttt caggtgcttt
     5341 ccaatcccaa acttccggat tgacttcgtc ggcaaattca ttgatggcgt taatcgccgc
     5401 ctgcatttga tcgtggccgt aaaccaccgc gcccaacatc acgtcttcgg gcaggatttt
     5461 ggcttcggat tccaccatca acacggcttt ggacgtaccg gcgaccacca agtccaattg
     5521 cgatttagcc aattcggctt tagtcgggtt caaaacgtac acgccgttta tataacccac
     5581 gcgtgccgca ccaatcggac cggcaaacgg tacgccgctc aacaccagcg cggcagacgc
     5641 gcccaacatt gcaggaatgt cggaatcgat ttcgggatcg acggacacga ccatcgctac
     5701 gatttggatg tcgtggtaga aaccttcggg gaacagcgga cgaatcgggc ggtcgatcag
     5761 acggctggtc aggatttctt tttcgctttg tttgccttcg cgtttgaaga aaccgccggg
     5821 gattttacct gcggcgtaag tgcgttccaa ataatcgacg gtcaggggga agaagtcttg
     5881 accttctttc acttctttgt tggtggtaac ggcaaccaaa acaacggtgt cgcccataga
     5941 gactttaacg gcagcggcgg cttggcgggc aatttcgccg gtttccaaag taacggtgtg
     6001 gttgccgtat tggaaagttt taacgtgttt gtcgaacatt attgttcctt tcaaaatacc
     6061 gcactgctaa aacactaata atgcacacta aaatccgaat gtgcatagtt agggtttcag
     6121 accgtgcggc aggttgtaaa caagctttca gacgacattt cggacgctga aagcaattgc
     6181 ggattataaa ggcaaccatc cttaaaatcc agtattgata caataaaaag gccgtctgaa
     6241 acatatctct ttcagacggc cttcaaactt aaaaatcaat cctgagtcag caaagtcaat
     6301 gcttcccgat acttctcgac agttttctga atcacatcgg caggcacttt cggcgcaggg
     6361 gcttttttgt tccaaccgct ttgttccagc cagtcgcgga cgaattgttt gtcaaaagac
     6421 ggcggattgg tgccgacttc atattgatcc gccggccaga agcggctcga atcgggagtt
     6481 aatacctcat ccatcagcgt cagcgtaccg tttgtatcca aaccgaattc aaatttggta
     6541 tcgcaaataa taataccgcg cgatttggca tattccgccg cttcggtgta aagccgaacc
     6601 gccttggcgc gcacttcttc cgccaattct ttgccgataa tgcgtccgca ttcttcaaag
     6661 ctgatgtttt catcgtgatc gccgactgcg gctttggtcg atggcgtaaa aatcacttca
     6721 ggcagttgtt gcgcttcctg cataccttcg ggcagccgaa taccgcaaac cgagccggtt
     6781 ttttgataat ctttccaacc gctgcctgcc agataaccgc gcacaatcgc ctctactttc
     6841 accggagtga gctttttagc cacgacggcg cgtttttcta aaactttggc ttcgttttca
     6901 ggcaaaacat cgtaaaccgt ttgaccggta aagtggttgg gcataatatg cgccagtttt
     6961 ttaaaccaaa aattggaaat ctgcgtcaga atctcccctt tgctcggaat cgggtcgtcc
     7021 aaaatcacat caaacgcgga caggcggtcg gaagcgacca tcagcatacg tttatcgtcg
     7081 atttcatata aatcgcgcac ttttccagaa tagatcttta ccaaaccaat ctcactcatt
     7141 tcgccccccc tgaaaatatc ttgaaaatac cgacccgaca cccgacaggt ttgaatcgca
     7201 aaccgatatt ctagccgaag tcggcgcaaa acaataccca tggcacaaaa agccaacccg
     7261 tcaaccgtcg gcaaagtttt ggcactataa taccgacagc aagtcctaca atacactttt
     7321 accaaaggaa atacctcatg agaatcctat tgacaggctc gaaaagccaa ctggcacgct
     7381 gcctgcgcga ccgtcttccg gaagactggg aaaccattgc gacggattcc gcatccctag
     7441 acattaccga tgccgatgcc gtctgcaaca tggtcaaaag tttccaaccc gacgccattg
     7501 tcaacacggc tgcctatact gccgtcgaca aagcggaagg cgatgcggca gcggcatttg
     7561 ccgtcaatgc ttccgccgtt tacaaccttg ccttggcagc acatcgcgcc catgcccgat
     7621 tcatccacat ctcaaccgac tatatctttg acggtaaagg gaaaaggccc tatcaggaaa
     7681 gcgactttac caatccttcc aatgtatacg gacaatccaa aaccgccggc gaactgctcg
     7741 cactgtctgc caatcccgac agccttatcc tgcggacttc ttggctgttt agcgaatacg
     7801 gggacaactt tgtccgcacc atgctgaacc ttgcgcggga acgttccccg ctgtccgccg
     7861 tccacaacca aatcggctgc ccgacctatg ccggcgacct ggctgccgcc atcatccgcc
     7921 ttttgcagca aacgtcgccc gtgcgcggca tttaccacta cgccggcggc aaatccgtat
     7981 cctggtacga atttgcccaa catattttcc aagcggcatc gcaacagcag acatccttcc
     8041 ccgttcccga attgactgcc gtttcagaca aggaatatcc gaccgccgcc cccaggcccg
     8101 catacagcat tttggactgc cgcaaaatcg aaaacgactt cggcatcaaa ccgtcagact
     8161 ggcagaaagc ccttgcacag gtcgtttcca agctgctctc tagtgggtac tagatataat
     8221 gcagcatccg ataaagtaaa gccccgaagt ggcagcttcg gggcttatcg gatagaaagg
     8281 aggcttgaag aaatggcgaa agttatttta ttcctgtttc ttttg
//
LOCUS       DAWHXZ010000079         8297 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000079 DAWHXZ010000000
VERSION     DAWHXZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.079"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(60..1505)
                     /gene="zwf"
                     /locus_tag="WIE35_001108"
     CDS             complement(60..1505)
                     /gene="zwf"
                     /locus_tag="WIE35_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ4154032.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWANNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            2021..3856
                     /gene="edd"
                     /locus_tag="WIE35_001109"
     CDS             2021..3856
                     /gene="edd"
                     /locus_tag="WIE35_001109"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ4154033.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            4037..4675
                     /locus_tag="WIE35_001110"
     CDS             4037..4675
                     /locus_tag="WIE35_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ4154034.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            4690..4908
                     /locus_tag="WIE35_001111"
     CDS             4690..4908
                     /locus_tag="WIE35_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154035.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(4932..5877)
                     /locus_tag="WIE35_001112"
                     /pseudo
     CDS             complement(4932..5877)
                     /locus_tag="WIE35_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            6040..7080
                     /gene="mutY"
                     /locus_tag="WIE35_001113"
     CDS             6040..7080
                     /gene="mutY"
                     /locus_tag="WIE35_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ4154036.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPIYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            7497..8057
                     /locus_tag="WIE35_001114"
     CDS             7497..8057
                     /locus_tag="WIE35_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ4154037.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPA
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT         2098 a         2302 c         1962 g         1935 t
ORIGIN      
        1 tgctttactt gcgttcagac ggcatttcaa accccatgcc gtctgaacgc attattgtat
       61 tactgctctt cgtgccactt gtgtccgtcg cgcgccaata gttcgcgcgc ggcttcaggc
      121 ccccacgagt gtgcgccgta gccgtgcggc ggcgtggtgt tatttgccca gttttccaaa
      181 atcggcatca catattccca cgcggcttcg agttcgtcgc ggcggttgaa caaagcgagt
      241 ttgccgttaa tcacatccag cagcaggcgc tcgtaagctt ccgcgcggcg gccttccaat
      301 gctttgccca aatcggttgc cagcggcacg gtttcgacct tatttcctgc ccccggggtt
      361 ttcatctgcg tatagaggcg cacggattca tatggttgca actcgataac gagccggttg
      421 ggcgcggtgc gactgccttc aaaaatatgg ctgttcaaat ctttgaagtt caaaacgatt
      481 tccgccactt tgcccgccat gcgcttgccg gtacgcaggt agaagggaac gcccttccag
      541 cgttcgtttt cgatttcggc tttaatggcg acgtaggttt cggtaaagct gtcttgcgga
      601 acgttgattt cttcaagata gccgttcatg cctttggcgg cggtatattg tccgcgcacg
      661 acattttcat tgacagattc gacggtcagc ggcttcaatg acttgatgac tttgactttt
      721 tcatcgcgca ccgcgtcggc atccaagctg gcgggggctt ccatcgcagt catgcacagc
      781 atctgcatca aatggttttg caccatatcg cgcaacgcgc cggtaatgtc gtaaaactcg
      841 ccgcgctctt ccacaccgag ctgttcggcg atggtcaact gcacgctttc gatgtattta
      901 ttgttccaca gcggctcgaa cattacattg gcaaaacgca gcgcaagcag gttttgcagg
      961 ctttctttgc caaggtagtg gtcgatgcgg taaatttgcc cttctttgaa ataacgcgca
     1021 acatcggtat tgatttgctg ggaagaagcc aaatccgtac ccaacggttt ttccaaaacc
     1081 acacgcacgt tgtcggcgtt caaaccgatt ttggcaaggt tttcacatgt ttgcgcgaag
     1141 aatttcggcg cggtggaaag atagatgacg acattgtcgg tttctttgcg cgctttgacc
     1201 aaatcgccca aagcggcaaa atcgtccggc tgcgtaacat cgactttgag atatgcgaga
     1261 cgttcgacaa acgatgccca agcctcatcg gaaaaatttt ctttcacatg gattttggaa
     1321 ctggtttcca ccttcgccag aaaaccttcg gtatccaact cgctgcggct gacccctaaa
     1381 atacgccctt cgggatgaag cagaccggca acatgcgcct ggtacagaca gggcaacagc
     1441 ttgcgcatcg ccaaatcgcc ggtcgcaccg aacaacacca aatcaaaatt tgtttgtgta
     1501 ctcatcgtat tatctcgtca ggaaagaatt tttcgatgcc gtctgaaacc tgtttccccc
     1561 atcacgctgc atcgcaatat cggaaacaaa ggcaggcggc ataatgagta gtaatactac
     1621 acaccgctac actttttgtc tattcccatt tttacaattt atttgaccta gtccaaaaat
     1681 cgggcaggtt tcccctattc cgttacaaca atcggaagat tctgcgattt aaatcaaatt
     1741 tcttttcgat gtctgatttt tttgtaacaa aattacaaat tttgtactat aatagcaccc
     1801 gcttcccact ttcagacggc atacctttta aaatatagtg gattaacaaa aatcaggaca
     1861 aggcgacgaa gccgcagaca gtacagataa tacggcaagg cgaggcaacg ccgtactggt
     1921 ttttgttaat ccactatact caccgtctga atacccgata caaaaatcag aaacgcacaa
     1981 acaaatcccc aatacccccc gttccgacag gagaccgacc gtgaacacta ctcctatcca
     2041 ctccaaactc gccgaaatca ccgggcgcat tattgaacgc agccgtccga cgcgtgaaaa
     2101 atatctggcg aagatccgca gtgccaaaca aatgggacgc ttagagcgca accagctcgg
     2161 ttgctccaac ttggcgcacg gctatgctgc catgcctaaa agtatcaaaa tcgaaatgct
     2221 tcaggaaacc gtccccaact taggcatcat caccgcctac aacgacatgg tttccgcaca
     2281 ccagccgttt aaagacttcc ctgaccaaat caaagacgaa gcgcagaaaa acggcgcgac
     2341 cgcccaagtc gccggcggca cacccgccat gtgcgacggc atcacgcaag gctacgccgg
     2401 tatggaactg tcgctgttct cccgcgacgt gattgccatg agtaccgccg tcgggctgtc
     2461 gcatcaaatg tttgacggca gcctgtttat gggcgtatgc gacaaaatcg ttccgggttt
     2521 gatgataggc gcgctttcgt tcggtcatat tccgggtatc ttcgtccccg caggcccgat
     2581 gtccagcggt atcggcaaca aagaaaaagc ccgcacccgc cagcttttcg ccgaaggcaa
     2641 ggtcggacgc aacgaacttt tgaaaagcga aatgggttct taccacagcc cgggcacctg
     2701 cactttctac ggcacggcaa actccaacca aatgatgatg gaaatgatgg gcgtgcacct
     2761 gcctgccgcc gccttcgtcc acccttacac cgacctgcgc gaagccctaa cccgctacgc
     2821 cgccggacac ctcgcgcgcg gcatcaaaaa cggcacgatt aaacctttgg gcgaaatgtt
     2881 gaccgaaaaa tcctttatca acgccttgat tggcctgatg gcaaccggcg gttcgaccaa
     2941 ccacaccatg cacctcgttg ccatggcacg cgccgccggc gtgattttga actgggacga
     3001 cttcgacgaa atttcctcca tcatcccgct gctcattcgc gtttatccga acggcaaggc
     3061 cgacgtgaac cactttaccg cagcgggcgg actgcctttc gttatccgcg aattgctgaa
     3121 tgcaggcctg ttgcacgacg atgtcgatac cgtcgtcgga cacggtatgc gccactacac
     3181 caaagagcct ttcctcatcg acggcaaact cgaatggcgc gaagcccccg aaaccagcgg
     3241 caacgacgac atcctgcgta aagctgacaa cccgttctcc cccgacggcg gtctgcgcct
     3301 gatgaaaggc aacatcggac gtggcgtgat taaagtgtcc gccgtgcgcg aaggctgccg
     3361 cattattgaa gcgcctgcca tcgtgttcaa cgaccaacgc gaagtgttgg ctgcgtttga
     3421 acgcggcgag ttggaacgcg attttgtgtg cgtcgtccgc taccaaggcc cgcgtgccaa
     3481 cggtatgccc gaattgcaca aactgacccc gcctttaggc atcctgcaag accgcggctt
     3541 caaagtggcg ctgctgaccg acggccgtat gtccggcgcg tccggcaaag ttccagcctc
     3601 catccacatg acacccgaag ccctgatggg cggcaacatc gccaaaatcc gtaccggcga
     3661 cctgatccgc ttcgactccg ttaccggcga actcaacgtc ctgattaacg aagccgaatg
     3721 gaacgcccga gaagtcgaaa gcatcgactt gggcgcgaac caacaaggct gcggccgcga
     3781 acttttcgcc aacttccgca gcatgaccag cagcgccgaa accggcgcga tgagcttcgg
     3841 cggcgaattt gcctgatgcg cgtttcagac ggccttttca gaccgaaggc cgtctgaaaa
     3901 attattcaag tgttttaaga tagacgtagg tcggattctc gaatccgaca cagccgtcca
     3961 agatgtcggt ttcttgaatc cgacctacaa cctgtcccat cttaataaaa taccccatcc
     4021 cacccggaga accgaaatgt ccaaactgac cccccgcgaa atcctgaccg ccggcgcagt
     4081 tgtaccggta atggcgattg acgacttgag tactgccgtc gatttgtccc acgcccttgt
     4141 cgaaggcggc atccccaccc tcgaaatcac cctgcgcacc cctgtcggcc tcgatgccat
     4201 ccgcctgatt gccaaagaag tgcccaacgc catcgtcggc gcgggtacgg ttaccaaccc
     4261 tgaacagctc aaagccgtcg aagacgcagg cgcggttttc gccatcagcc cgggtttgca
     4321 cgaatccctc gccaaagccg gccacaacag cggcatcccc ctgattcccg gtgttgccac
     4381 cccgggcgaa atccaactgg ctttggaaca cggcatcgac accctcaaac tcttccctgc
     4441 cgaagtcgtt ggcggcaaag ccatgctcaa agccctgtac ggcccttacg ccgatgttcg
     4501 cttctgcccg acaggcggca tcagcctcgc caccgcgccc gattacttgg cactgcccaa
     4561 cgtcctgtgc gtcggcggct catggctgac gccgaaagaa gccgtgaaaa acaaagactg
     4621 ggacaccatc acccgtctcg ccaaagaagc ggcggcattg aaacccaaag cctgaaccgc
     4681 atcgtaaaaa tgccgtctga agccctttcc agtctcagac ggcattttgc cgcttggcgg
     4741 cgcagtcggc atacacggca gcaccactcc gacatactgc ccctaaaatg cccatccgcc
     4801 ttccgcataa taaaaataac gttcagttca ttcgacagca gccggacagc ccatactacg
     4861 cggctgaaaa aatgccgtct gaaacgcatt cagacggcat ccgcttaaaa aaacaactga
     4921 ttcaaggccg attaatccgc ttccaaaacc actttcatcg cttgattttc ggcggcgtgt
     4981 ttgaacacgt cgtaggcttt ttccaattcg ctgaatttga aacggtgggt caacattttg
     5041 gtgtaatcga cggagctgct ggaaattgcc ttcatcagca tttcggtggt attggcgttt
     5101 accaggccgg tagtgatggc aagcttttta atccacagtt tttccagttt gaaatcaacg
     5161 gattgaccgt gtacgccgac aacggcgata tggccgccgg gtttcacaat gtcttggcac
     5221 atattccaag ttgcagggat accgacggct tcgatcgcgc agtccacgcc gtcttcgccg
     5281 acgatagcga caacttgctt gcttacatcg ccggaagcag ggttaatggt atgggttgcg
     5341 cccaactctt tcgccagttt caaacggttt tcgtccatat cgcaaacaat gatggcggcg
     5401 gggctgtaca gttgggcggt cggcaaggcg tcgctcaaca acagggcgac ttcttcgttg
     5461 acgttgtcgg gcagcggcac gaggctgttg tcggcataag gcgtgcggac gtattctgcc
     5521 tgcgtgccgt caatcatgta gcccaaaatc cagccgccgt tgcggcaatg cgaataaagt
     5581 tggattttgc agttgtcgca agtacagcat ttgctgacgc atgaaataat gactttatcg
     5641 ccgactttga tgttttttac agcctcgccg acttcttcta caataccgat gccttcatga
     5701 ccgagaatac ggccgtcggc gacttcgggt ttttttgcct ttccaaatac ccaaatcggt
     5761 accgcaaatc gtggttttga cgattttcac caccgcatcg gtcggatcga taatctgcgg
     5821 acggggtttt tcttcaaaac ggatgtcgtt tgcgccgtga taaaccattg ctttcatgct
     5881 gatactcctt gcttgttgat aaataatttc aataccgcaa taaagtttcc ttatatgagt
     5941 tatatgcccc tacaaaaaat aagtcaataa gaattatttt cacaatgtta tacaataaca
     6001 tactatttta aatataaata aaaccaccga ttgatattaa tgaacacacc catccccttc
     6061 tccgaacggc tcatccgctg gcaaaaacaa cacggtcgcc accacctccc ttggcaggtc
     6121 aaaaaccctt attgcgtctg gctttccgaa atcatgctcc agcaaacgca agtcgccacc
     6181 gtgttggact actatccgcg cttcttagaa aaattcccga ccgttcagac gcttgccgcc
     6241 gcgccgcaag acgaagtgtt gtcgttgtgg gcgggcttgg gctattacag ccgcgcgcgc
     6301 aacctgcaca aagccgcgca acaagtcgtc aggcaattcg gcggcacgtt tccgtcggag
     6361 cgcaaagact tggaaaccct ctgcggcgta ggcagaagca ccgccgccgc catttctgcc
     6421 ttctccttca accgccgcga aaccattttg gacggcaacg tcaaacgcgt actctgccgc
     6481 gtgttcgccc gcgacggcaa tccgcaggac aaaaaatttg aaaactcgct ctggacactt
     6541 gccgaaagcc tgctgccgtc tgaaaacgcc gatatgcctg cctatacaca aggtttgatg
     6601 gatttgggcg cgaccgtgtg caaacggacg aaacccttgt gccaccaatg cccgatggcg
     6661 gacatctgcg aagcaaaaaa gcaaaaccgc accgccgagc tgccgcgcaa aaaaaccgcc
     6721 gccgaagtac cgaccctgcc gatttactgg ctgattgtcc gcaaccggga cggcgcgatt
     6781 ttgctggaaa aacgccccgc caaaggcatt tggggcgggc tgtattgcgt gccgtgtttt
     6841 gaaagtttga acgggctttc cgactttgcc gccaaattct ccctgaccat ggcagatatg
     6901 gacgaacaaa ccgccctgac ccaccgtctg acgcaccggc tgctgatgat tacgccgttt
     6961 gaaggacaaa tgccgtctga aagcccttca gacggcattt ggataaagcc ggcgcatttg
     7021 aaagattacg gcctgcccaa gcctttggaa atttatttaa acggtaatag gttagaataa
     7081 acaaaataaa cccattgaac tgttgtttgc aggtatcgca gcaagaacaa ccgatgaatt
     7141 tgggtcgtat tttaggcggt gggataatgt tcaaatggga catttggaac ggaagaagtc
     7201 ggcaatttaa aaaggattta aaaagcaaag aaggtcaaaa acatgaacac aaacttaaat
     7261 gacaaagaca aagccatgga taccgcaatc aggtttcaga aaaggatgag gattccgaaa
     7321 tttttctttt taattctctg aatcacaatg gttttggcat ttatccaaga cgtgataacg
     7381 ggttctaatt ttctgcaaat aacaattaat gtaaaatttt cgtaaaaatt tatcggcttt
     7441 taaaacaaaa ttgactaaaa tagtcgcgag tttttactgc aataaaggag attgcaatga
     7501 atatgaaaac cttattagca ctagcggtta gtgcagtatg ttcagttggt gttgcgcaag
     7561 cacacgagca taatacgata cctaaaggtg cttctattga agtgaaagtg caacaacttg
     7621 atccagcaaa cggtaacaaa gatgtgggta cagtgactat tactgaatct aactatggtc
     7681 ttgtgtttac ccctgattta caaggattaa gcgaaggctt acatggtttc cacatccatg
     7741 aaaacccaag ctgtgagcca aaagaaaaag aaggtaaatt gacagctggt ttaggcgcag
     7801 gcggtcactg ggatcctaaa ggtgcaaaac aacatggtta cccatggcaa gatgatgcac
     7861 acttaggtga tttacctgca ttaactgtat tgcatgatgg cacagcaaca aatcctgttt
     7921 tagcaccacg tcttaaacat ttagatgatg ttcgcggtca ctctattatg atccacacgg
     7981 gtggtgataa tcactccgat catccagctc cacttggcgg tggcggccca cgtatggcat
     8041 gtggcgtgat taaataattc gattgttcga aacgaaaagt gcggtgaatt ttgaccgcac
     8101 ttttttgcta gatatttagc atttaattca cgacttcagc agtatagtga attaaattta
     8161 aaccagtaca gcgttgcctc gccttagctc aaagagaacg attctctaag gtgctgaagc
     8221 accaagtgaa tcgattccgt actatttgag acctttgcaa aattcctttc cctcccgaca
     8281 accgaaaccc caacaca
//
LOCUS       DAWHXZ010000080         8098 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000080 DAWHXZ010000000
VERSION     DAWHXZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.080"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..573
                     /locus_tag="WIE35_001115"
     CDS             <1..573
                     /locus_tag="WIE35_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224706.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ4154038.1"
                     /translation="DLGKLSPEAQLAAASLLQDSAFAVKDGINSARQWADAHPNITAT
                     AQTALAVAEAAGTVWRGKKVELNPTKWDWVKNNGYKTPAARPMQTLDGEMAGGNKPVV
                     KSIRPTTRDELRQALQEQGFRRTGSDEAQYETWKGPDGVKIDIRPNGEVIRTQRVPRT
                     DGVQGKYPQRQNYEGNPLPNNRHHSGYFIK"
     gene            570..887
                     /locus_tag="WIE35_001116"
     CDS             570..887
                     /locus_tag="WIE35_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154039.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            957..1697
                     /locus_tag="WIE35_001117"
                     /pseudo
     CDS             957..1697
                     /locus_tag="WIE35_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            1701..2102
                     /locus_tag="WIE35_001118"
     CDS             1701..2102
                     /locus_tag="WIE35_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154040.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            2261..2603
                     /locus_tag="WIE35_001119"
                     /pseudo
     CDS             2261..2603
                     /locus_tag="WIE35_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2743..3099
                     /locus_tag="WIE35_001120"
     CDS             2743..3099
                     /locus_tag="WIE35_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ4154041.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNKKTVIFHF"
     gene            3272..3646
                     /locus_tag="WIE35_001121"
                     /pseudo
     CDS             3272..3646
                     /locus_tag="WIE35_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3684..3946)
                     /locus_tag="WIE35_001122"
                     /pseudo
     CDS             complement(3684..3946)
                     /locus_tag="WIE35_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4602..5588)
                     /locus_tag="WIE35_001123"
     CDS             complement(4602..5588)
                     /locus_tag="WIE35_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ4154042.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            complement(5628..6428)
                     /locus_tag="WIE35_001124"
     CDS             complement(5628..6428)
                     /locus_tag="WIE35_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ4154043.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLNTYYADRFNQTERGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            6553..7896
                     /gene="argG"
                     /locus_tag="WIE35_001125"
     CDS             6553..7896
                     /gene="argG"
                     /locus_tag="WIE35_001125"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ4154044.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKK"
     gene            7971..>8098
                     /locus_tag="WIE35_001126"
     CDS             7971..>8098
                     /locus_tag="WIE35_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ4154045.1"
                     /translation="MKEYKVVIYQESLLSSLFFGATKVNPVNFSAFLNKQTPEGWRV"
BASE COUNT         2329 a         1759 c         1843 g         2167 t
ORIGIN      
        1 gatttaggaa aattaagtcc ggaagcacaa cttgctgccg cgagcctatt acaggacagt
       61 gcttttgcgg taaaagacgg catcaattcc gccagacaat gggctgatgc ccatccgaac
      121 attacagcta ctgcccaaac tgcccttgcc gtagcagagg ccgcaggtac ggtttggaga
      181 ggtaaaaaag tagaacttaa cccgactaaa tgggattggg ttaaaaataa cggctataaa
      241 acacctgctg cccgccctat gcagacgttg gacggtgaga tggcaggagg aaacaagcca
      301 gttgttaaat ctatcagacc aactacgcga gatgaattac gtcaagcatt gcaagaacaa
      361 ggttttagac gtactggttc agatgaggct caatatgaaa catggaaagg tcctgatggc
      421 gtgaaaatag atattcgtcc aaatggagag gttataagaa cccaaagagt gccgcgaacc
      481 gatggtgtac agggaaaata tccgcaacga caaaattatg aaggcaatcc attgccaaat
      541 aatcgtcatc attctggata ttttatcaaa tgaaaaaaaa tatttttcac aatgtaagcc
      601 tttatgaaat aatcttttcc gataatggaa atacccttac attatctttt acagatacaa
      661 ttgaaggtaa ttatttcgga tatatcaaat gcagtaatat tttgaatttt aaattagata
      721 caaataattt cgtagattat gaggataagg aagatagctt gtttcccttg tttatacccg
      781 aaatagagct atataaatac caattttata gtgaaattat tattgatgta gggattatta
      841 taaaaatatc tgctgaaaca attaattttg agccactggg aaaatagtaa ctgctttccc
      901 agcagccgta gcaactgtat ttttacccga cggggtaaaa atacagttgc tacatctctg
      961 cgcagcagac tctacaaggt attaataatt taggaaaatt aagcccggaa gcacaacttg
     1021 ctgccgcgag catattacag gacagtgctt ttgcggtaaa agacggcatc aattccgcca
     1081 gacaatgggc tgatgcccat ccgaatataa cagcaacagc ccaaactgcc cttgccgtag
     1141 cagaggccgc aggtacggtt tggagaggta aaaaagtaga acttaacccg accaaatggg
     1201 attgggttaa aaataccggc tataaaaaac ctgctgttcg ccatatgcag actaaggcgt
     1261 taggtacggt agatgaaatt ggcgatacag tacagcaggt tgggaaacag gctagcggac
     1321 aaaaaaccag cggtggtaat cctgcgattg atagcgaccc ctatagcccg agtagtgtgg
     1381 cagctcgcat agaaaccggt aaggtgcgca gtgatttaca aatcaaagac attttgagca
     1441 atactactca aaggagtaaa acaaaaggtc ccgctgttca gtatgataaa gtgggggatt
     1501 acaatgacgc actaaatgat tttaatagtc tgaatgttcg aaatgtacaa acacgtccta
     1561 atggaacgat aacgggcaat ttacctgata ggcgtgcggt taatgctcgt aatgatagta
     1621 gtggtggaga accaacactt gaaataacaa ttagtaataa ccgaaaaata aaaatcagat
     1681 atggaaatac acgataaatt atgaaattaa aaagcttaga tttcccaact ggctatttct
     1741 attttgataa tgcagcaata aactctgata aagtagaagt tatagcagtt ggttatagaa
     1801 atacggataa aaccataaaa atttttattg aagatgttat tcattttagg gttgttgatg
     1861 aatcgtattt tatagatact tttatggatt taatttcgga agatgcagat agagctttgc
     1921 ttcatgaaaa tggtggtcaa tctttttttg aacttcttga tgagtgttat gcggaatgga
     1981 tattgaaaga aagttatttt cctttgaata gagaattctt taaatactat atttttatgt
     2041 ttgagcaaac attcatagaa ataattggtt ctagtgcaac gtattcaatt attgagggct
     2101 agcgtaagat gagtaataag ttgcctatct ttctttcagg cagcctgaaa ataaaactac
     2161 ccaagttgat ggtgtacctg tatcagtgaa gggaaatttt gttgatggta aatttcgcat
     2221 tggtacggca acaatgaaat cattttaaat tgagctagaa atgaacctag aaaattatga
     2281 aaacatttta ataaaattac ttttttatca taacaactta gtaaatgaat attcttattt
     2341 tattgaaaat aaaaatttat ttaaaatagt atctcgaact aaaacgagta agggcttttt
     2401 ttcactatag aaaaaccatt aaattttcta agcaaaaaaa cttattttga gtttaatttt
     2461 aaatatttac actcagggaa agaacgcttt ggttcgttta tgtgctggat aaatactaat
     2521 ttaatggaat ttgagggggt ttttttaacg acctgctccc tgataatatg ataataaata
     2581 acttttttga aataaatgat taacgataca ccaataaaaa ttggtggggt aaccgtatgg
     2641 atgatattcg cggcatcatc caaggtgcgg ttaatccttt aatttacaag gtaagcaagc
     2701 aaaagattat ttacaacaaa ctcatatcag gaacttagac aaatgaatga acacaacctg
     2761 ttaattttct gtttaaaaga caatgtttca attagtgaat atactgaaat gattgattgg
     2821 gcttataaaa atattcaatc tgaaacagtt gtagaaatta cggaaagtca aattattgaa
     2881 tatcaaaatc gtggattatg gagacttgtt tctgaaatta ccgataattg gttatttgga
     2941 ccaagtgagg gggattggct aatagataag gaaagtattt tggctgtaaa agaaaaatta
     3001 caaaattcag atttttctac agagccctta gtgaaaaata ttattcatgt acttgaatat
     3061 gctataaaaa ataaaaaaac agtaattttt catttttgag actaatccaa tttttagtaa
     3121 tattgatgca gagcaagcag cattagatgc cgcaaacatg gggagaagct attcaattta
     3181 gaattaaaaa acaaattgaa aatgaactag caccaccaaa ttggtctacc cagtttccta
     3241 atggtagtat ttatgatcct aaggtaacga aatgattatt caaaatgaat ttaatttata
     3301 tcctagtaat atgcttcctg aaaggttttg ttatcctgaa aagtatgttc gtatctctaa
     3361 cgatacatct ttaatacctt atattcagcc acataatttt cactggtagt ttgagaatta
     3421 tggaacagaa ggggcagaag tagcttatat atttagaaat tctatcctgc ctgatttaaa
     3481 tcttatccca ttcgctagta atggagaatg ggaagcttat tttgatggta atgatgtaac
     3541 aggaaattct agggttattg tcattaattt agataatata gaaaaccatg aattttttaa
     3601 tagttttgaa gattggcttg aattagcaat taaggatact tggtaagcag ctatctataa
     3661 agagatgagg ctgccttgga caactaggat aaactcgatt ttactaattg ttttaaaatg
     3721 gaacaagaac ttttatttca ctgttgttaa aacgccattc gcactccttt aaatacagct
     3781 caaaatgcgc tttgggaatg ccgttaaact tgcgtaaatg acgttttgct tgattccaaa
     3841 agttctcagt tccattaata tggttttgtc gttcggcaaa atgtgtgctg tgattgatac
     3901 ggaaatggct aaattcgccc gcatccaata catcatagcc acgataacaa aatgagttta
     3961 ttttgtttat accgtcttag acgactttct ctcataggga taattctaac ttaatttgaa
     4021 tttccctagt gatctagggc agcccctaaa ttaataaagc agcacaactc cttttgccga
     4081 tgttccggac tgtcaaacga ctgttcctca tgccacatct ccatcaaggt acggataacc
     4141 cgctccgcct taccgttggt ctgcggacaa gcaaatcggg caagcctcca accaatccca
     4201 ttattataac aagctgcacc gaaagcatgt tggacggctc tttatattac ctatcattgt
     4261 cagagtaaac gtactcaatc aggtacaagc aggggtcagc cagatgttcg gtcagaaact
     4321 tggcagcact gtctgcggtt ttgtccggca aaatggcaga gtataaaaat cgtcaatagc
     4381 gacaaacagg taatctcgtt tatcggcggc cttctgtcct ttgagcaaca acaaccgatc
     4441 ggtatcagga tgaacaaaac ctcccgggac aacctgcctt ttacggcttt aagtgcacgg
     4501 taaatagtga cgcggctgac ttagtggcag catactgggg aggtgagtgt ttttgtgtat
     4561 atttttattt tggtattccc ttagaaatac tgtaaacaac gctaccggac ggcctgcagg
     4621 gcttcgcgca cgcttgcttt gagttctgcg ccgaagcgtc tgcccaagat tctgccgaaa
     4681 tcgtccttcg gagtgtaatc caccacatcg ggggctttga ccacgtctcg cgccacgctg
     4741 taaatattgc cgagtccgtc caccagcccg actttcagcg catccgcgcc tgtgtacacg
     4801 cgaccgctga acacgtcggg atattgtcgg aatttgaggc ggccgccgcg tccggttttg
     4861 acggctttga tgaactcacc gtgtatgccg gtcagcattt cttcccagat ttttgactgt
     4921 tcgggcgttt cgggcgaaaa cggatcgccc atgcctttgt tgctgcctgc aattttaacc
     4981 ctgcgtttca cgccgatttt ttccatcagg ccggtcgcgt cgaaactgct gccgataacg
     5041 ccgatgctgc cgacgatgct ggacgggtcg gcatagattt tgtccgccgc cgccgcgatg
     5101 tagtagcagc cggacgcgca catatcttcc gccacgagat aaacgggaat gccggggtgc
     5161 tgcgccttca gacggcgtat ttcttcaaaa gcggtgttgg agacgacggg cgaaccgccg
     5221 gggctgttgg cgcggatgac gatggctttt gcctgcgggt ttttgtaggc ggcctccata
     5281 ccgtctttga gttttttgac ctggtcttct acaccgttgc cgatttcgcc gtacaggttg
     5341 acgactgcgg tatgcggcgt gttgcccgcc aactgcaatg cggcttcgtc ttttcggaaa
     5401 atgcctgcaa tcagggcaac cagaatcagg gtgctgacgg cgcgccagat gtttttccac
     5461 atccgctccc tgcgcctgtc ctgataggcg gacagcagca cttcgcgcat gatgtcgcgc
     5521 tcccataagg tttcccccgc attttttgct tcgggtgctt cgttttctct tctgattcgg
     5581 tattgcatgg ttttccttaa atattgtccg atttgggcaa acggttttca gtttacccga
     5641 tttttcagct ctgctcccaa tccgtccaag ctgtgcaaca cttccgccca cgccgcgtcc
     5701 aaaaggttga cggcttctcc ttcggctttg atgccgaact caatgtgcgg tttgacctgc
     5761 gtgccgtctg aatgcgtcca accgacgctg ggcaggctgt acgaacgcac accgggataa
     5821 gtttgctcga tatgctccat aagcggcgta atgcgcgatt cgggctgctc aaacacatac
     5881 acgctgcggc tgccgcgttc ggtttggttg aagcggtcgg cgtaataagt attcaaaacc
     5941 cattccgcca tcgggtgcgc catcacagga aagccgggga agaaataatg ctcgcggatg
     6001 gaaaatccgg cgatgttgtt gaacgggttg ggcacaagct ctgcgccctc ggggaaatcc
     6061 gccattttca ggcgttgggc gtgttccggc gaatcaagcg gctcgccgcg tttctgggtt
     6121 atgccttcga taaacttggc ggcttcagga tggcggacga caggcaaatc caaagcagca
     6181 gccgcggctt ggcgcgtgtg gtcgtcgggc gtggcgccga tgccgccggt aacaaacgtc
     6241 ggtatgccgt ctgaaaagct gcggcgcagt tgcctgacca gtaaatcggg ttcgtcgggt
     6301 agatattgca cctgattgag cttcagccct ttggattcga gcagggattt gaaaaaggcg
     6361 aaatgcttgt cttggcggct gccgtgcagg atttcgtcgc cgatgatgat gaggttgaac
     6421 gcgttcataa tgttttcggt tttaaatacc gcctgaaacg gcggaatacc cgttcagacg
     6481 gcatgatggc tgacaatatt tcctatttat aagataatgg ctttgatttt tcaagctacc
     6541 gaggattttt tcatgaacca aaaccatact attttacaaa atctccccgt cggtcagaaa
     6601 gtcggcatcg ccttctccgg cggacttgat acctccgccg cgctgttgtg gatgaaactc
     6661 aaaggcgcgc tgccttatgc ctacactgcc aacctcggcc agcccgacga agacgattac
     6721 aacgccattc ccaaaaaagc gatggaatac ggcgcggaaa acgcccgctt aatcgactgc
     6781 cgcgcgcagt tggcacatga aggcatcgcc gccatccaat gcggcgcgtt ccacgtttcc
     6841 accggcggca tcgcctattt caacaccacg cctctgggcc gcgccgtgac cggcaccatg
     6901 ctcgtttccg caatgaaaga agacgatgtg aatatttggg gcgacggcag cacctacaaa
     6961 ggcaacgaca tcgagcgttt ctaccgctac ggtttgctca ccaatcccgc gctgaaaatc
     7021 tacaaaccct ggctcgatca gcaatttatc gacgaactcg gcggccgtca cgaaatgagc
     7081 gaatttctga ttgccaacgg cttcaactac aaaatgtcgg tggaaaaagc ctactccacc
     7141 gattccaata tgttgggtgc cacccacgaa gccaaagact tggaattttt gaactcgggc
     7201 atcaaaatcg tcaaacccat tatgggcgtt gccttttggg acgaaaacgt cgaaatcgaa
     7261 cccgaagaag tcagcgtacg ctttgaagaa ggcgtgccgg ttgcactgaa cggcaaagaa
     7321 tacgctgatc ccgtcgaact cttcctcgaa gccaaccgca tcggcggccg ccacggtctg
     7381 ggtatgagcg accaaatcga aaaccgcatc atcgaagcca aatcgcgcgg catctacgaa
     7441 gccccgggaa tggcgttgtt ccacatcgcc tacgaacgct tggtaaccgg catccacaac
     7501 gaggacacca tcgaacaata ccgcatcaac ggcctgcgcc tcggacgttt gctctaccaa
     7561 ggccgctggt tcgacagcca agccctgatg ttgcgcgaaa ccgcccaacg ctgggtcgct
     7621 aaagccgtta ccggcgaagt taccctcgaa ctgcggcgcg gcaacgacta ctcgattctg
     7681 aacaccgaat cgcccaatct gacctaccaa cccgaacgcc tgagtatgga aaaagtcgaa
     7741 ggtgcggcgt ttaccccgct agaccgcatc ggacagctca cgatgcgcaa ccttgacatc
     7801 accgacaccc gcgccaaact gggtatctac tcgcaaagcg gtttgctctc gctgggcgaa
     7861 ggctcggtat tgccgcagtt gggcaataag aaataaggtt tgctgttttg catcattagc
     7921 aacttaaggg gtcgtctgaa aagatgatcc cttatgttaa aaggaatcct atgaaagaat
     7981 acaaagtcgt catttatcag gaaagcctgt tgtccagcct gtttttcggc gcgacaaagg
     8041 tcaaccccgt caatttcagc gcgttcctca acaaacaaac ccccgaaggc tggcgggt
//
LOCUS       DAWHXZ010000081         7993 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000081 DAWHXZ010000000
VERSION     DAWHXZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.081"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(263..2953)
                     /gene="glnE"
                     /locus_tag="WIE35_001127"
     CDS             complement(263..2953)
                     /gene="glnE"
                     /locus_tag="WIE35_001127"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ4154046.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYGAYEAMRKLHRQISS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPASPEQQQLLAESMGFDSYAAFSDDL
                     NAHRTKVNQLFNEILSEPEEQTLDTSEWQWAWQEKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAEQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHTQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCSLIDKNLAEQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWRDVFGEEAATA"
     gene            complement(3151..3486)
                     /locus_tag="WIE35_001128"
     CDS             complement(3151..3486)
                     /locus_tag="WIE35_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154047.1"
                     /translation="MPSEALSGLQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WNGVAKAAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKSAAY
                     AILKERGEV"
     gene            complement(3500..3973)
                     /locus_tag="WIE35_001129"
     CDS             complement(3500..3973)
                     /locus_tag="WIE35_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ4154048.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4026..4307)
                     /locus_tag="WIE35_001130"
     CDS             complement(4026..4307)
                     /locus_tag="WIE35_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154049.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(4394..4717)
                     /locus_tag="WIE35_001131"
     CDS             complement(4394..4717)
                     /locus_tag="WIE35_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154050.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(5051..6058)
                     /locus_tag="WIE35_001132"
     CDS             complement(5051..6058)
                     /locus_tag="WIE35_001132"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ4154051.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNMVYKLGLIPVTGNRTKPV
                     KLMGLDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPNPQPGNPQPRLFRVPE
                     YQGIINRMGFNNHGIDAMIQNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGRDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     Q"
     gene            6290..6526
                     /gene="acpP"
                     /locus_tag="WIE35_001133"
     CDS             6290..6526
                     /gene="acpP"
                     /locus_tag="WIE35_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ4154052.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            6682..7929
                     /gene="fabF"
                     /locus_tag="WIE35_001134"
     CDS             6682..7929
                     /gene="fabF"
                     /locus_tag="WIE35_001134"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ4154053.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
BASE COUNT         1846 a         2129 c         2064 g         1954 t
ORIGIN      
        1 tagaatgcgc tattttaatg gaaacggcgg ggttttaaac ggttcttatt tgtttgtttt
       61 gatttttgct tgggtaaaaa atcccatcat tcccacaaaa acagaagcct gaaatcccgt
      121 cattcccgcg aaagaggaat ccggtttttg ggtttcagcc attcccgata aatcgcttta
      181 gctctgccgc aaccttaaaa cggcacggta cgaaaatacc gtctgaaacc cagattgtca
      241 ggcttcagac ggcatttttt gttcaggcgg ttgccgcttc ttcgccgaac acgtcccgcc
      301 acaatttcct gacattgccg taatgtgcca acagttcgcc ggttacttcg gtttttgccg
      361 cgtcgcgcag tttggtgttg tgctgctgcc ggcggtagaa gcggtaggcg gtgcggcttt
      421 gttcggcgag gtttttgtcg ataaggctgc agtcggcggc gatgtttaag agggcgatgt
      481 tgccgtagtt gtccaagagt tgcggatact ggcgggcatg ggcaagtatc agatattgga
      541 cgataaattc gacatcgacc acgccaccgc gcgcgtattt gacgttgctg tcggcaggcg
      601 gatgggtggg gaacattttt tcgcgcattt cgataatttc gcctgctaag gcggtttggt
      661 cgcgttcggc agtcagcatt tcggtgcgga tgcggtcgaa ggccgtctga atttcgggtg
      721 tgccgcagat gaagcgggcg cgggtgaggg attggtgttc ccacgtccag gcgttttcgc
      781 gctggtattt ttcaaaggcg gcgatgctgt gggcgaggaa accggcgtcg ccattagggc
      841 gcaggcgcag gtcggtttcg tagaggctgc ctgcgccggt ggcggcggaa agccagttgg
      901 tcaggcgacg ggcgaggcgg ctgtacacgt cgcctgcgtc ggggtgggga tcgtcgtaga
      961 gatagacgag gtcgaggtcg gaggcgtagc cgagttcttt gccgccgagt ttgccgtaac
     1021 caacgacggc gaattgcggg gtgtcgcggt gttttttggg catgtccgcc cacgcgcaca
     1081 gcagggcggc ggcgaggatg gtgtcggcga gggcggagag ttggtcggag agggattcta
     1141 ccgtccacag tccggcgagg tcttggacgg cgagacggaa gacttgggtg tgctggaagc
     1201 ggcgcagggt gtccatttgc gcttcagtat cgccgccgca ggctttgagg tcgtctgaaa
     1261 gggcggcggc gagtgcctgc caatcaaacg cggtatccaa aagctgcgcg ctgatgagtt
     1321 cgtccaacaa aatcggatat ttgttcagat acgccgccac ccaagaactt tggcccataa
     1381 tctgcgccag ttgcgccaag gtttgcggat gttcgttgag gaaggcgaga taggcggatc
     1441 ggcggctgat gttttcgaga aaatccaaca gccgcatcaa tgtatcggtc gggttgcttt
     1501 gctctgccgc cgcctgtacg aacagcggca caaccgcatc gaaacgcggc tgggcgtgtg
     1561 cggaaagatg gcggtattta tggccgtggc ggatttggtc gagccttgcg gcgacggttt
     1621 cggcatcgaa cccgtgcgcc ttcagacggc atcgccgccc ttcttcgtcg ggcttttcct
     1681 gccatgccca ttgccattcg ctggtgtcta gggtttgctc ttcgggttcg ctcaaaattt
     1741 cgttgaacaa ctgattgact ttggtgcggt gggcattgag gtcgtctgaa aaagcggcgt
     1801 agctgtcgaa acccatactt tcggcaagca gttgctgctg ttcgggcgaa gccggcaggg
     1861 tttgggtttg ccggtcatcc cagtattgca ggcggtgttc gacatcgcgc aggaatcggt
     1921 aggcggcaag cagggtttcg acgttttcag acggcatgat cccggtttcg gcaagtttct
     1981 tcagcgtttc ctgcgtgcct ttcagttgca gcgcacgcat ttggccgccg cgtatcatct
     2041 ggaaaatctg ggcgataaat tcgacttcgc ggatgccgcc cgcgccgagc ttgatgttgt
     2101 ccgccatgcc ttttttgctg acttcgctgc tgatttggcg gtgcagctta cgcatcgcct
     2161 catacgcgcc gtaatccaga tatttgcgga acacgaaggg gcgcaccagc gatttgattc
     2221 cgttcggata cggcgtaacc acgcggcctt tgcaccacgc gtagcgttcc cattcgcgcc
     2281 cctgcgtaat caggtattgc tccagcgcgg tttcgctcaa caccaacgcg cccgaatcac
     2341 cgtccggccg cagccgcata tcgacgcgga acacctgccc gtcggcggtg atgtcgttca
     2401 acagcgcaat cagtttctgc ccgactttgg tgaaaaactc ctgattgccg cgttcgcgcc
     2461 tgccgtcggt gtcgcccgat tcgggataga cgaaaatcaa atcgatgtcg gaagacacgt
     2521 tcaactcata gccgcccgcc ttgcccatcg ccaccacgct caaatgctgc ggcgatttgg
     2581 tataacgccc gatcggcgtg ccgtacatgt cccgataata ggcgtaggca aaatccagcg
     2641 cggtattgac ggcaaaatcg gcaaacagcg taatcgtgcg ggttacttcg ttcaaatcgc
     2701 tgatgcggtt tatatcgcgc acgataatct gcgacaccac ataacggcgc aacccgcgca
     2761 actgccgcgc caattcttcc tcgttttctt ccgcacggat tttgtcccag tcggcaaagg
     2821 cttggaaatc cgcttcggtc aaaaccttgt ccagcatagg caggaaaatt tcgggcttga
     2881 gtttgccgtt gtcgagctgg cgggcgagga agagggaatg gcggcgggcg gtgtcgaggc
     2941 ggttgtcgga catttcggat tccgtttgaa aaaatggcag gaagatgatg gcggattcac
     3001 ttcaaatcag gacagggcga ggggatgccg taccggttta aagtcaagcc actatatcat
     3061 agcaaccttc gatgccgtct gaaaccttgc cccgacatcc agacggatgc ctgccgataa
     3121 aaaacagcgg caaatgccgc cgtttcctgt tcacacctcg ccacgctctt ttaaaatagc
     3181 ataagccgca cttttctccc gtgtggaatt tttaaaaaaa ccatcactct tgacgatttc
     3241 atgcagctcc tcacttgatt tctccgcata ttccattttc aacgccttat gctcattgcc
     3301 ggcttcaatc attccctctc ccactgcttt tgctgctttt gccacaccat tccaaaaacc
     3361 cataatcatg tcctccaatc gtttcaaata aataaggtcg ttgtttctgt caagaaacaa
     3421 cgacagttta taaactgtcg gaaaacaaaa tgccgtctga aggccggata atgcttcaga
     3481 cggcatcgga ttgccggttt taaccctgcc ctccgccgtt gttcaaatag tgttcccagt
     3541 gagaatataa ccgggcaata ttttcttcat aggtgtgggc agaaggaata taaccgtctt
     3601 tgggcggcat atacctgaca aatgattcta tttcttccgg ataatcaaaa tctgcataaa
     3661 ttttttcaac ttcccccaaa ggatcctcaa attcttcttt tctatggaat acatcgctta
     3721 agaggacgta cagccaattt ttaggggaat aatccctcat ttccgaagcc aaatcttcca
     3781 gaacaggctt taattcaaga atattggatt tgttgattaa agatagtttg aacacacgtt
     3841 catcatgatc tgaaagcgtc atttttcggg cataagctgc cacatcagcc caacccaagt
     3901 atttattccc atacccccac agaatatcct tccaagaaag acggactttt tgcgcggtta
     3961 aatcaaggtt cattttttac tccgattgcc tgccccgtca ttccgcccct tccgattgac
     4021 gcctgttacg aaaagtcttc caaatgacgg ctgattgcct cttcaacagt cggatgccaa
     4081 tccataaatg aagcctgcaa ttcctctaaa accaactcat tgaataaatc atccaaattc
     4141 cgccaatccg ttttttcatc cggatagatg aaatttgcca taacgacagt atgtttgttt
     4201 tgggaataat ccgataaggc gcaaatcatc agataccgcc ccgaatgctc cagataaaaa
     4261 attcgttttt ccaaaggaaa ccggttattc cgttgcccgt tttccatttt tcatcctttt
     4321 taacgtatcg gcttccgcgt ccttcaaatt gagttacctg ccttccgcca agcaaacgcc
     4381 cgcgcccctt tcatcatttg aatatccgtt tgaccatatt taccagataa ctatcttctt
     4441 tatataaatc aatcagcaaa ggcacgatac ccggctcata aaataattct gtcgtatcag
     4501 atgtttcttt taacgcggaa attatctcca acagttcata cccgtatcgg ggataagcaa
     4561 acaaataggg gactgcttcc cgcaaacatg aaaatgcttc gtaactttgc atttcccata
     4621 tatgttcatc tttaaaatca ctcaaatacc attcatccaa ttcactttcc ggatcggata
     4681 tcaatcggat aaattcattt agttcgtcct taaacatatc aaatccttaa atataaaatc
     4741 ttttgattat ccgccgtatc cttattttcc acatcatgaa tttcaacgcc gttcatattt
     4801 tgcgttttct caaaccgtcc accacggcat aaaattattt tttcatatca attatttata
     4861 tgtttaacaa cttaccgccg gacttgcttt ggctgcgtat caagattgtc cggttcgcgg
     4921 cgggcgtttg ctttgtttca aacagggcgg cgcgtaaggg attttcaaag ggcggcaggc
     4981 aggaaaagga aataaaaatg ccgtctgaac ggcaaaacgt gcgttcagac ggcattttgt
     5041 gcgggacgcg tcattgcgcc aaagccttca aacattcttt gaccaatgcc ggacctttgt
     5101 atatcaatcc gctgtacacc tggacggcag ttgcgcccaa gcggattttc tctgccgcgt
     5161 ccctgccttc cataatgccg cctacgccga taatcggcag cttgccgtcg atgtggtctg
     5221 ccaacagctt caacacccga ttactttttt catgaacggg cagcccgctc aaaccgccct
     5281 gctcgcctgc gagcggatgg ctgccgagac ttgatttgtc gatggtggta ttggtagcga
     5341 tgatgccgtc catttcgacg gatttgacaa cgtgggcgat gtcttcgatt tgtgcttcat
     5401 ccaaatcggg ggcgattttg acggcgagcg ggacgtattt cccgtgtaca gaggcaagct
     5461 gtgcctgttt gtttttcaaa gcctcaagca atgcgctcaa ctcgtcgcca ccttgcagcg
     5521 cgcggaggtt tttagtgttg ggcgaggaaa tattgacggt aatgtaactt gcgtgtgcgt
     5581 aggctttttc gaggcagatt aaataatcat cggcagcgtt ttcaatcggt gttacagcgt
     5641 ttttgccgat gttgatgccc aatacgccgc tgaatttact tttttcgatg ttttgaatca
     5701 tggcatcgat gccgtggttg ttgaagccca tgcgattgat gatgccttga tattcgggaa
     5761 cgcggaagag gcgcggctgc gggttgccgg gctgcgggtt gggcgttacc gtgccgattt
     5821 cgatgaaacc gaagccgagc gcgcccaatg cgtctatata ttcgccgttt ttatcgagtc
     5881 cggcggcaag cccgacaggg ttgggcaaat ccaaacccat cagttttaca ggtttggtac
     5941 ggttgccggt tacgggaatc agacccaatt tataaaccat gttgagcgcg tcgagcgtga
     6001 agtggtgggc tttctcggca tcgagtgcaa acaggatgcg gcgggcaagt ggatacatga
     6061 cagggctttc tgcttcagat ggaaatgccg tcattttaac cgaaatcccg tcaaacatac
     6121 ggcacacagg tcaaacttga acgacaaaac ccgccgcacc caaaaatgta caaatcggca
     6181 aatattgata taatagtcat tagatagaaa actctattaa aagtcggtac aataccaccg
     6241 atttatttcc cttaaacaac ctgccgcaag gcataaagga acgactgata tgtcaaacat
     6301 cgaacaacaa gttaaaaaaa ttgttgctga acaactgggc gtaaacgaag ccgacgtgaa
     6361 aaacgaatct tctttccaag acgacttggg cgcggattct ttggacaccg tggagctggt
     6421 tatggctttg gaagaagcat tcggctgcga aatccccgac gaagatgccg aaaaaatcac
     6481 caccgtccaa ctggctatcg actacatcaa tgcccacaac ggctaaccgg tcgtcgcccg
     6541 acacaacagc ctctgctgca ccgcgcaata gaggcttttc ccttatatgg caaactgttt
     6601 gaacccctgc cgcaaattac agacggcatc tgcacgacag ccgcatatgc ccccattccg
     6661 acaaccaaca gcgaggttat catgagtcag agaagagtag tcattacagg cttaggtcag
     6721 gtttcccctg tcggcaacac tgtcgcagag gcttgggaca ccctgctcgc cggcaaaagc
     6781 ggcatcggcg cgattacccg ctttgacgca tccgacatca acagccgtgt cgccggcgaa
     6841 gtgcgcggtt tcgacatcgg acaatacatc agcgcgaaag aagcgcgccg gatggacgta
     6901 ttcatccact acggcattgc cgccgcattg caggcaatcg ccgattcggg cttggacgat
     6961 gtggaaaacc tcgacaaaga ccgcatcggc gtgaacatcg gttccggcat cggcggactg
     7021 cccagcatcg aggtcaccgg caaagccgta atcgaaggcg gcgcgcgcaa aatcaaccct
     7081 ttctttatcc ccggttcgct gatcaacctg atttccggac acgttaccat cctcaaaggc
     7141 taccgcggcc cgagctacgg gatggtttcc gcctgtacca ccggcgcgca cgccatcggc
     7201 aattccgccc gactgatcaa atacggcgac gcggacataa tggttgccgg cggcgcggaa
     7261 ggcgcaatca gcactttggg cgtgggcggt tttgccgcga tgaaagccct ctccacccgc
     7321 aacgacgacc ccgccaccgc ttcccgtccg tgggacaaag gccgcgacgg cttcgttatc
     7381 ggcgaaggcg cgggcatatt ggtgttggaa gaattggaac acgccaaaaa acgcggcgcg
     7441 aaaatctacg ccgaaatcgt cggcttcggc atgagttccg atgcttacca tatcaccgcg
     7501 ccgaacgaag aaggccccgc ccttgccgtt acccgcgcgc tgaaagatgc cggcatcaat
     7561 cccgaagacg tggattacgt caacgcgcac ggcacgtcca cccccttggg cgatgccaac
     7621 gaaaccaaag ccctcaaacg cgcgttcggc gaacacgcct acaaaaccgt cgtcagctcg
     7681 accaaatcca tgaccggcca cctgctcggc gcggcgggcg gcgtggaggc cgtgtacagc
     7741 attttggcga tacacgacgg caaaatcccg ccgaccatca acatttttga acaagacgtt
     7801 gaagccggct gcgatttgga ctactgcgcc aacgaagcgc gcgacgcgga aatcgacgtt
     7861 gccatttcca actccttcgg cttcggcggc accaacggca cgctggtctt caaacgcttc
     7921 aaaggctgat tccgcaaagc cgccgccgac atcgaaatgc cgtctgaaac cgtttcagac
     7981 ggcattttta tag
//
LOCUS       DAWHXZ010000082         7948 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000082 DAWHXZ010000000
VERSION     DAWHXZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.082"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..387)
                     /locus_tag="WIE35_001135"
     CDS             complement(<1..387)
                     /locus_tag="WIE35_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154054.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDPETDKFAKV"
     gene            complement(371..688)
                     /locus_tag="WIE35_001136"
     CDS             complement(371..688)
                     /locus_tag="WIE35_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ4154055.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEKVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
     gene            complement(816..1094)
                     /locus_tag="WIE35_001137"
     CDS             complement(816..1094)
                     /locus_tag="WIE35_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154056.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            complement(1101..1385)
                     /locus_tag="WIE35_001138"
     CDS             complement(1101..1385)
                     /locus_tag="WIE35_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154057.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            complement(1393..1590)
                     /locus_tag="WIE35_001139"
     CDS             complement(1393..1590)
                     /locus_tag="WIE35_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154058.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            complement(1595..1885)
                     /locus_tag="WIE35_001140"
     CDS             complement(1595..1885)
                     /locus_tag="WIE35_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154059.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQPVKKG"
     gene            complement(1930..3273)
                     /locus_tag="WIE35_001141"
     CDS             complement(1930..3273)
                     /locus_tag="WIE35_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ4154060.1"
                     /translation="MEDKQGMTKAVVGVMTDALADGRKPATASNLPPLSNRGGTETET
                     AGRVQEVFECYETYITDGKGNLLGVPLRRGVSDSAFIDQISFSFHEKTFFDKYGVRVS
                     LLEDEDFIRAASMLAEEVFGFGIYKESKGSGGRFYERCWLMGSEDVLYGRVHFGGQNN
                     TILFELTGTGCGVAKEGWESRLFAFLTNAIRPKITRVDIAKDFFNGEYSPNQAREDRN
                     KGMFTCHHVKPKGECLGSDWEEDDEAKMTKGKTYGIGSRESSKYVRVYEKGKQLGDKT
                     STWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAYPICERFVQKATRIHAVKEDKVISAD
                     RYLEWVKKQFGRAANGLKFIFPELDKAKLFELIEPSHHKLPKSLAPEAYDCAFLKAQA
                     IHEQPAFKPYKDPYYMYEYYENLEKQLEQQKHVNNEESYNNFIYDKFARLPISWA"
     gene            complement(3358..3573)
                     /locus_tag="WIE35_001142"
     CDS             complement(3358..3573)
                     /locus_tag="WIE35_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154061.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYIFRP"
     gene            complement(3861..4214)
                     /locus_tag="WIE35_001143"
     CDS             complement(3861..4214)
                     /locus_tag="WIE35_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154062.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            4450..5877
                     /locus_tag="WIE35_001144"
     CDS             4450..5877
                     /locus_tag="WIE35_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ4154063.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            5946..7349
                     /locus_tag="WIE35_001145"
     CDS             5946..7349
                     /locus_tag="WIE35_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ4154064.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(7513..>7948)
                     /locus_tag="WIE35_001146"
     CDS             complement(7513..>7948)
                     /locus_tag="WIE35_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ4154065.1"
                     /translation="GFDRYLFRPYARSLKGQIVKAQISGKRYQRLSLVSAQVGNRLIA
                     PMVYQNTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAEKLGHKVLPL
                     APYSPELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         2012 a         1887 c         1941 g         2108 t
ORIGIN      
        1 gacctttgca aatttgtcgg tttcggggtc gtattggtag cctcgtgcct gtatgtcttc
       61 tttgaaagtt tcgtatacgt catgggctaa aagggctgtt ccgacatagg gaaccgccct
      121 tgtgctgaat ttcgcgccta agcgggcaag tttgccgccc cccgccaata cgccggcgcg
      181 ggaaatgctg gcggttattt tggcgttgat tcgggctttt gcgcccgtag ggatgtgtgt
      241 taaatctacc gtttttatta aatcagatga ataagtttta ctatttttag gtacaaactt
      301 atgaattttc gcaccttgtc cggtatcaac tgaaacagtt tcagatattt ttactgcatt
      361 tgcattcgct tcaaacgaat acatcatcaa aattgcaatt atcgacaatt tcgcaaaatt
      421 caaatttgta tattttatga ccatctttca gggattcttt aattaccttt tctgaattat
      481 cagaaaatga gattagccaa atatcatgtt taattcttct attccagaaa aaagagaaac
      541 aatcaataac attttcagac ttattaatct tcgcaaattc aacaaattca gattgcgcta
      601 taaccgccat cgattgccca aaatacttgc tagacggctg atatttataa agtgccaact
      661 gcgcctgagt gataaacggc ttgttcatgg ttctgccttt caatgattgt tttgaaagcc
      721 tgattttgac accataactt catgcgctca attcttaaac agaaccgccc cgattaatac
      781 gggtacggaa acgccgagat aaaaataaaa atccatcatt tcaaaacctt tttcagcagg
      841 gaaacaaagt aaacggacgc gaggatgccg aatactatcc aacctgtttc aagaccgctt
      901 tgcaggttgt ctttcggact gcattccgcc aataaaagcc ttagcggctg accgtccgac
      961 atcttccaca ggctgccgtt atattccggc ctgacaatct gtccgttttc tttgattctt
     1021 ggtactacca agctgaaata aaggttttca gcctggtgct tctcaagaca tttatttccg
     1081 acttggtagt acatgccgtc ttacttcatc actctcttaa cgatggaaaa cacaaaaagc
     1141 gcggcgaaaa cgccaacgac aatccaaccg gcttccatac cgtccgcttt tgcggcttcc
     1201 aaagcgtttt ttgccgtatc gggcaacgtt gcatttgcat gtgcggccaa agccagggga
     1261 gcagctgtta caacagccag ttttgcgccg tatttacggc aggtgttaat aaatttcatg
     1321 atattttcct ttaaaaagtg tttggcggta atggatggag cgtttttcag acgaccgccg
     1381 aacatccgaa aatcagtctt tcaaaaatcc gaatacgaca aattcgtatt ggttgccgat
     1441 ttcttccaaa cctgcgttaa tcgcttcttc gaagtcgtag aaataatcgg cattggtgat
     1501 taatttggta tgtccgatgt cgcccgtttc aggagagaga tacagaaagt cccctgttga
     1561 tacggactgg acaacataga ctttctgcat tcaatcagcc tttcttaacg ggttgaaaac
     1621 cgatgacttt cagtttttgg gttttgcccg tagtgacgat ttctacgttc aggtttgctt
     1681 cgatcggaaa ttgggcgttt cggaactgct cgaaattggc agagccgccg aaatcgtatt
     1741 cagtagtaga gctgcccaat gcgttgcctt gggagctgtc taagggtgtg gcgacaatca
     1801 ggcggcaata gtcgaagctc ttgccttcga tttgtccgtt gaatttttta acgccgacga
     1861 tgtggccttg aagttggatg ttcatttttt ggtttccttg tgtgattaaa cgtctttcgg
     1921 gcagacactt taagcccatg aaatcggtag tcttgcgaat ttgtcgtaaa tgaagttgtt
     1981 atagctttct tcattgttga cgtgtttttg ctgttcaagc tgtttttcaa gattctcgta
     2041 atattcgtac atatagtaag ggtctttgta cggtttgaat gcgggctgtt catgaatggc
     2101 ttgagctttc aaaaaggcgc agtcgtaggc ttcgggagcc aaagacttgg gcagcttgtg
     2161 atgactcggc tcaatcagtt caaacagttt ggctttgtcc aattcgggaa aaatgaattt
     2221 cagaccgttt gccgcacgtc caaactgttt ttttacccat tcaaggtagc ggtcggctga
     2281 aatgacctta tcttccttaa ccgcgtgtat gcgcgttgcc ttttggacga atcgttcgca
     2341 aatcggatat gcgccgccga aatattcgcc cggattctgc aaaacttcaa aagggataac
     2401 gatgtctttt gctttgaatt caatttcaaa gcgcgtccat gtgcttgttt tatcgcccaa
     2461 ctgcttgcct ttttcataga cgcggacata tttggacgat tcacgggagc cgataccata
     2521 ggtcttgcct ttggtcattt tggcttcatc gtcttcttcc caatctgacc ccaaacattc
     2581 gccttttggt ttgacgtgat gacaggtaaa cataccttta tttcggtctt cacgggcttg
     2641 gttcgggctg tattcgccgt tgaaaaagtc ttttgcgatg tcaacgcgtg tgatttttgg
     2701 gcggattgca ttagtcagga atgcgaaaag tcgtgattcc cagccttctt ttgcgacgcc
     2761 gcaaccggtg ccggtcagtt cgaaaaggat ggtattgttt tgtccgccaa aatggacgcg
     2821 accgtatagg acgtcttccg aacccatcaa ccaacagcgc tcataaaaac gaccgcccga
     2881 gcctttggac tctttgtaga taccgaaacc gaaaacttct tcggcgagca tggacgcggc
     2941 gcgaatgaaa tcttcgtctt ccaaaagact tacacgaacg ccgtatttat cgaaaaaggt
     3001 tttttcatga aatgaaaagc taatttgatc aatgaaagcc gaatctgata caccgcgccg
     3061 aagaggaacg cctaacaggt ttcctttacc gtccgttatg tacgtttcgt aacattcgaa
     3121 gacttcctga accctgcctg ccgtttcggt ttctgtaccc cccctgttag ataagggggg
     3181 aagatttgaa gcggttgccg gcttcctgcc gtccgctaac gcgtccgtca tcacgccgac
     3241 aaccgccttt gtcatccctt gcttatcttc catggtgcga atcctcaaaa acgggcaaaa
     3301 aaaagccctg ttacttgtag aaagtaaagg gcgttaattt ttgttaatcg tcccttctta
     3361 gggacgaaat atataaggtt ttaaaacgct aagaacaaaa tccgataacg gctctgaaga
     3421 agtaagtctt tctttattgc acaagatagc aaacttccac ggcattctcg ccccacccgt
     3481 gccgtccaag cctttttcaa agacatagat ggttttcttg gcaatcactt cgtcagtttg
     3541 gcggtcgata atccgtatgg tcgcacctgc cacccagtgc ctgcgcagct tggaatcgac
     3601 attgttttcg aacgttaccg cataacgggc ggggtgtata tgattaagta tttgatttat
     3661 cggaaaatct ttacctgaat accgaataat atcggaatgg ttgggttgca ggacatccac
     3721 atagcggtat tccccatcgc taaaaaatcc taggaaacga gcaataaaat ttacgccttc
     3781 gctggtctgt aaagccgcat tgtcccacat cgggtctctg gtttttgcat ctgccgaaac
     3841 ggtacgctca ggtactacct tcaacagcat aatcccttcc acattgtccg ccgtctggta
     3901 aatctttccc ccgccgtttt gcattgttcg ttaaacacgg cttcggcttc tttgtatttt
     3961 ctgtcccact cttcttgtgc ctgtatttct tctttgatcg ggccgaattg tttgggaata
     4021 atccaaacaa acagcatcag gatagcggcg gcggtcaggc tgcctgaaag gattttgccg
     4081 gggttccgtt tgggcttttt ataggcaaag cggacgagaa accaaagcaa cagcagcatg
     4141 gtgccccaat agccgattga gaataggatg gccaaacctt ctaggaaatg gcgtaaatcg
     4201 tttgtggtaa acatgggttg ttcctgtggt taaatgtgca ggctgctttt tgccgaacct
     4261 tgccgcatct caaaagcagc ctgcgcttca gcgttgcgtt acgcagtaaa ataatgaata
     4321 tttgtaacga cttgggtatt ttttgtcaat attcccgcct ttcccttaac agctgccgcg
     4381 ctttccgtta aaattccttt acatatttat attgtttcct gtttctatat tgccaaggtt
     4441 atacccgtta tgtttttctc cgccctgaaa tcctttcttt ctcgatacat tactgtatgg
     4501 cgcaatgttt gggcggtgcg cgaccagttg gaaccgccca aacgcacggc ggaagaacag
     4561 gcgtttttgc ccgcgcattt ggaactgacc gatacgccgg tctctgccgc tccgaaatgg
     4621 gcggcgcgtt ttattatggc gtttgcgctt ttggctttgt tgtggtcctg gttcggcaaa
     4681 atcgatattg tggcggcggc ttcgggcaaa acggtgtcgg gcgggcgcag caaaaccatc
     4741 cagccgctgg aaacggcggt ggttaaggcg gtacatgtgc gcgacgggca gcatgtgaaa
     4801 cagggagaaa cgctggcgga actggaggct gtgggaacag acagcgatgt ggtgcagtcg
     4861 gagcaggctt tgcaggctgc ccaattgtcc aaactgcgtt atgaagcggt attggcggca
     4921 ttggaaagcc gtaccgtgcc gcatatcgat atggcgcaag cacggtcttt aggtctctcc
     4981 gatgccgatg tgcaatcggc gcaggtgttg gcgcagcacc agtatcaggc atgggcggcg
     5041 caggatgcgc aattgcagtc ggctttgcgc ggccatcagg cggaattgca gtcggccaag
     5101 gcgcaggagc agaagctggt ttcggtgggg gcgatcgagc agcagaaaac agcagactac
     5161 cgccgtttgc gggccgacaa ttttatttcg gaacatgcgt ttttggagca gcagagcaaa
     5221 tcggtcagca attggaacga tttggaaagt acgcgcggtc agatgaggca gattcaggcg
     5281 gccattgcac aggcggagca gagtcgggtg ctgaatacgc agaacctgaa acgcgatacg
     5341 ctggatgcgc tgcgccaggc aaacgaacag attgaccaat accgcggcca aacggataag
     5401 gcaaagcagc ggcagcagct gatgacaata cagtcgcctg cggacggcac ggtgcaggaa
     5461 ttggccacct atacggtggg cggtgtggtg caggctgccc aaaaaatgat ggtggttgcg
     5521 cccgatgacg acaaaatgga cgtggaagtt ttggtattga acaaagacat cggttttgtg
     5581 gaacagggac aggatgcggt ggtgaagatt gagagttttc cctatacgcg ctacggttat
     5641 ctgacgggca aggtgaaaag tgtcagccat gatgcggtaa gccacgaaca gttgggcttg
     5701 gtttatacgg cggtggtgtc gctggacaaa cataccttga atattgacgg caaagcagtg
     5761 aatctgacgg cgggcatgaa tgtcacggcg gagattaaaa cgggtaaacg gcgggtgctg
     5821 gattatctgt taagcccgct gcaaaccaaa ttggacgaaa gctttaggga gcgataggcg
     5881 gatccgtact gggcatttgt tatccgccgg ttcggacatg cagactgcct gaaaccattg
     5941 cccggatgac attgctcaat ctaatgataa tgcaagatta cggtatttcc gtttgcctga
     6001 cactgacgcc ctatttgcaa catgaactat tttcggctat gaaatcctat ttttccaaat
     6061 atatcctacc cgtttcactt tttaccttgc cactatccct ttccccatcc gtttcggctt
     6121 ttacgctgcc tgaagcatgg cgggcggcgc agcaacattc ggctgatttt caagcgtccc
     6181 attaccagcg tgatgcagtg cgcgcacggc aacaacaagc caaggccgca ttccttcccc
     6241 atgtatccgc caatgccagc taccagcgcc agccgccatc gatttcttcc acccgcgaaa
     6301 cacagggatg gagcgtgcag gtgggacaaa ccttatttga cgctgccaaa tttgcacaat
     6361 accgccaaag caggttcgat acgcaggctg cagaacagcg tttcgatgcg gcacgcgaag
     6421 aattgctgtt gaaagttgcc gaaagttatt tcaacgtttt actcagccga gacaccgttg
     6481 ccgcccatgc ggcggaaaaa gaggcttatg cccagcaggt aaggcaggcg caggctttat
     6541 tcaataaagg tgctgccacc gcgctggata ttcacgaagc caaagccggt tacgacaatg
     6601 ccctggccca agaaatcgcc gtattggctg agaaacaaac ctatgaaaac cagttgaacg
     6661 actacaccga cctggatagc aaacaaatcg aggccataga taccgccaac ctgttggcac
     6721 gctatctgcc caagctggaa cgttacagtc tggatgaatg gcagcgcatt gccttatcca
     6781 acaatcatga ataccggatg cagcagcttg ccctgcaaag cagcggacag gcgcttcggg
     6841 cagcacagaa cagccgctat cccaccgttt ctgcccatgt cggctatcag aataacctct
     6901 acacttcatc tgcgcagaat aatgactacc actatcgggg caaagggatg agcgtcggcg
     6961 tacagttgaa tttgccgctt tataccggcg gagaattgtc gggcaaaatc catgaagccg
     7021 aagcgcaata cggggccgcc gaagcacagc tgaccgcaac cgagcggcac atcaaactcg
     7081 ccgtacgcca ggcttatacc gaaagcggtg cggcgcgtta ccaaatcatg gcgcaagaac
     7141 gggttttgga aagcagccgt ttgaaactga aatcgaccga aaccggccaa caatacggca
     7201 tccgcaaccg gctggaagta atacgggcgc ggcaggaagt cgcccaagca gaacagaaac
     7261 tggctcaagc acggtataaa ttcatgctgg cttatttgcg cttggtgaaa gagagcgggt
     7321 tagggttgga aacggtattt gcggaataaa gcaggctgaa acggttatga aattcccaaa
     7381 gcagcctgca ccccgtttcg aaagtgcagg ctgctttggg attgatccga tactttcaca
     7441 ttctcattgc ctttaaataa agcacagtta aaaacacatt taaattaagt gcgaattaat
     7501 tcaaatgtat attcaattaa aatcaaaata ggacagtagt gcatcgtcaa atcgggcgta
     7561 atcagacaaa acggttcgca gataccgctt aatattcgcc cacactttct caatcgggtt
     7621 gagctcaggt gaataaggtg caagaggcaa taccttatgt cccaattttt ccgccatttc
     7681 ccgtaagaca cccatacggt gaaatcgcgc attatctaaa ataatcaccg atttttgagt
     7741 caatgcgggc agtaggcatt gctgaaacca cgcttcaaaa aagactccgg tcatcgtatt
     7801 ttgataaacc atcggagcaa tcagccggtt gccgacttgt gcggacacca aagataagcg
     7861 ttggtatctt tttccactta tctgcgcttt cactatttgc cctttcaggc tgcgggcata
     7921 gggacggaac aggtagcggt caaatcct
//
LOCUS       DAWHXZ010000083         7923 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000083 DAWHXZ010000000
VERSION     DAWHXZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.083"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            331..1644
                     /locus_tag="WIE35_001147"
     CDS             331..1644
                     /locus_tag="WIE35_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ4154066.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILMPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1865..2022
                     /locus_tag="WIE35_001148"
                     /pseudo
     CDS             1865..2022
                     /locus_tag="WIE35_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            2035..3285
                     /locus_tag="WIE35_001149"
     CDS             2035..3285
                     /locus_tag="WIE35_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ4154067.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTERGRIAV
                     MQESEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLAKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEIFADETEIYTLVKTFVDNAIRYTPSEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERVRVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(3320..4813)
                     /gene="rng"
                     /locus_tag="WIE35_001150"
     CDS             complement(3320..4813)
                     /gene="rng"
                     /locus_tag="WIE35_001150"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ4154068.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEKTQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAASNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            5026..5283
                     /gene="grxC"
                     /locus_tag="WIE35_001151"
     CDS             5026..5283
                     /gene="grxC"
                     /locus_tag="WIE35_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ4154069.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5304..5747
                     /gene="secB"
                     /locus_tag="WIE35_001152"
     CDS             5304..5747
                     /gene="secB"
                     /locus_tag="WIE35_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ4154070.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSSTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5833..7875
                     /gene="recG"
                     /locus_tag="WIE35_001153"
     CDS             5833..7875
                     /gene="recG"
                     /locus_tag="WIE35_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ4154071.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLQHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
BASE COUNT         1658 a         2048 c         2347 g         1870 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta taaaatccca cgtgtacggc acgccccgcg
       61 ctccgcaatg ataggggcgg tttcgtttga atcggcgtac cgggatgaat gcccccgcgc
      121 cgtcccgata ccggtttgcc ggttgttccg tatcggttgc cgccgtgaaa tgacggacgg
      181 atacaggaat ggcggcaggc gtaaatggga ggaaatgacc gattgacttg tttcaaaata
      241 aaaaagtaga atgcaaggct tatcccgcgt ctgtgggcgg ttttgcctgc acggtcggtt
      301 ttctgtttaa aataaaataa aggagaaatg atgctgacgt ttatcggact gctgattatc
      361 ggggtcatcg tatggttgct gctgacggaa aaagtgtcgc ccatcatcgc attaatcttg
      421 atgccgctgt ttggggcgtt gctggcgggg tttgatgtat cccaattaaa agaattttat
      481 tcgggcggca ccaaatcggt gatgcagatt gtgattatgt ttatgttttc cattttgttt
      541 tttggaatca tgaacgatgt ggggctgttc cgtccgatga taggcggttt gattaagctg
      601 actcggggta atatcgtggc agtgagtgtg gggacggtct tggtgtcggt ggtggcgcag
      661 ttggacgggg cgggtgcgac gacgttttta ttggtcgtcc ccgccctttt gccgctttac
      721 aagcgtctgc atatgaatcc ttacctgctg tttttgctgc tgacttccag tgcgggattg
      781 attaaccttc tgccgtgggg cgggccgact gggcgggttg caagcgtgtt gggcgcagat
      841 gtgggcgaat tgtataaacc tttgttgacg gtgcaaatta tcggtgtagt gtttatcctt
      901 gcgctgtccc tgcttttggg tgtgcgtgaa aaaaggcgga ttgtccggga gttgggcgcg
      961 ttgcccgccg tggcggattt gataaagccg gtgcctttgt cggaagaaga acaaaaattg
     1021 gcgcgtccga aactgttttg gtggaatgtc ctgctgtttt tggcggcgat gagcctgctt
     1081 ttttcgggta tcttcccgcc gggttatgta tttatgctgg ctgcaacggc ggcgttgctt
     1141 ttgaattacc gcagcccgca ggaacagatg gagcggattt atgcccacgc cggcggcgcg
     1201 gtgatgatgg cgtccattat tttggcggca ggtacgtttt tggggatttt gaagggtgcg
     1261 gggatgttgg acgcgatttc caaagacatt gtgcatatcc tgccggacgc gctgctgcct
     1321 tatctgcata ttgccatcgg tgtgttgggc attccgcttg agttggtttt gagtacggac
     1381 gcttattatt tcggactgtt tccgattgtg gagcagatta cctcgcaggc gggcgtggcg
     1441 cccgaagcag caggttatgc gatgttgatc ggcagtatcg tcggcacttt tgttacgccg
     1501 ctttcgccgg ctttgtggat gggcttgggt ttggcgaaat tgtcgatggg caaacacatc
     1561 cgttattcgt ttttttgggc gtggggtttg tcgctggcga tattggccag ttcgatagcg
     1621 gcaggaatcg tgcctctgcc gtaaacggcg aagccgtctg aagcccgaat cgttcctgtg
     1681 gtctgtcgca ggctgcctgc ctgcccgtgt gttgcaatcg cgccccaatg ccgtctgaag
     1741 gcagttgccg gcgcggtttt tttgtgctgc agttttgaaa aataccggca agcgtaaagg
     1801 ccgctaaatt gcggctaatt ttggggtgtt agaataggca cggtttgtct gaaagggaaa
     1861 gttgatgcgt gttttgctgg tggaagacga tgcgatgatt gcggaggcgg tgtcggcaag
     1921 tttgaaagac ggcggctatg cggtggattg ggtcaaaaac ggcgcgcggc tgccgctgcc
     1981 cagccttatg acttgatgct gctggatttg ggtttgccgg ggcggacggt ttggatgttt
     2041 gttccgctgg caatgcttgc aggtatgttt tcctactacg aaaccttcca cgaaacggaa
     2101 gcgttgcagg acgacctgct ccgtcaggcg gcattgtacg tcgcccccga ttccaaaccc
     2161 gaaactttgc ccgagggcga cggcgatacg cgtattttgg tacagatgcc gcagcaggaa
     2221 gaccctgttg tcagcctgcc tgcgcatctg gcggacggtc tgcacacgct tcaggcggac
     2281 ggggacgacg attattaccg cgtctatatc cgcacgacgg agcgggggcg gattgccgtg
     2341 atgcaggaaa gcgaataccg ggaagatttg gcggcggatg cggcacggca aagcgtgttg
     2401 cccctgctgg cggcactgcc gctgatgata ctgctgaccg tgtggattac gcacaaagcc
     2461 atgcgccccg tccgcaaatt gtcgcaaagt ctcgaacaac gccgaatcaa tgacctgtct
     2521 gccttgagtg tggacaatat tcccagtgaa atcagagggt tcgtaaccgc catcaacttg
     2581 cttttgaaac gtgcggatga agacatccgc caccgtcagc ggtttgtcgc cgacgcggca
     2641 cacgaattgc gtacgccgat gacagccctt tcccttcagg cggaacggct caacaatatg
     2701 tctctgccac ccgatgcggc gcggcagtct gccgttttgc agcaaagcat acggcgcaac
     2761 aaacacctgc tcgagcagct tttggcactg gcgcgttcgc agtcggacga aacccctttg
     2821 gcgaaaacga cattcggtct gcaaagccgt ttccgccaag tgttgcagga actgatgccg
     2881 cttgccttgg aaaaacgtca ggacattggt gtggcggtcg gaggtgatgt cgaaatattt
     2941 gccgatgaaa ctgaaatcta taccttggtt aaaacgtttg ttgataacgc aatacgttat
     3001 accccgtccg aaggcaggat agatttgggt ttcacagacg aagggaaata tcttgccgtg
     3061 tgggtggaag ataacgggaa cggcattccg gaatccgaac gcgtccgcgt cctcgatccg
     3121 ttttaccgta ttttgggaac ggagcagcag gggacggggc tggggctgtc gattgccgac
     3181 acgctagcca aaaaatacgg cggatatttg gaactgaccg acagccgcag gttcggacac
     3241 ggtctgctga tacgcgcctt gttggacaag gaaaccctga aatagacaga tgccgtctga
     3301 aggcttcaga cggcattttt tacatcagca caatatcgta ttgttcctgc gtgtaagcgg
     3361 tttcgaccgc cagagaaatc ggtttgccga tgaaatctat cagcattgcc aaggattgcg
     3421 attcttcgtc caaaaacaaa tcgatgacgt tggaggcggc gaggatgcgg aaactttcgg
     3481 catcgtaacg gcgcgcttcg cggacgattt cgcgctggat ttcgtagcac acggtttgcg
     3541 gcgttttcag acggcctctg ccttggcagg aagggcaggg ttcgcagagg acttggttta
     3601 agttttcgcg cgagcgtttg cgcgtcagct cgaccagccc caggctggta aaaccgtgca
     3661 gggtaacgcg ggtacggtcg aaggcgaggg ctttggcaag ctcctgcaac acggcttcgc
     3721 ggtggctttc ctgcgccata tcgatgaagt cgatgatgat gatgccgccg aggttgcgta
     3781 gcctcaattc gcgggcgatg gtgtggcagg cttcgaggtt ggtgcggaag atggtttcgt
     3841 cgaagttgcg tgcgccgacg aagccgccgg tgttcacgtc tatcgtggtc atcgcttcgg
     3901 tggactcgat aatcaggtag ctgccgaagt tgaggttgac gcgcggttgc agggcgcggc
     3961 tgatttcctg ctcgacgttg tgggtttcaa acagcgggcg ttcgcctttg aacaattcta
     4021 tcctgcccaa tgcgccgtgg acgtattgtt cggcaaaacg cgtcatccgt ccgtggttta
     4081 cggtggaatc gacgaggatt ttttgcgtgt cgcagccgac catatcgcgc aatacgcgca
     4141 aactcaaggg caaatcctga taaagcaggg tttccggcgg ccggattttc gcctgttctt
     4201 ggatgtgttc ccacactttg gtcaggtagt cgatgtcgga ctggagctgt tcgtcggtgg
     4261 cgttttcggc gttggtgcgg atgatgtagc ctcggcaggc attttccggc aggagcttgt
     4321 cgaggcgttc gcgcaggctg ctgcgttcgg catcgtcttc gatgcgttgg gacacgccga
     4381 tgtggtcttc ttgcggaaga tggacgagga aacgccccgc cagcgagatt tgggtggaaa
     4441 gccgcgcgcc tttggtgttg atcgggtctt tgatgacctg caccaaaaca gactgccctt
     4501 caaacagcat atgttcgatg cgctgggttt tttcggggtt gcggcgttgt tcgaggacat
     4561 cgacgatgtg taaaaacgcc gcgcgttcca agccgatgtc gataaacgcg ctctgcatcc
     4621 cgggcagcac gcggcgcacc acgcccagat agatattgcc gaccaggctg tgttcgctgt
     4681 tgcgctcgat gtgcagctcg cagatattgt tttcctccaa caccgctacg cgcgtttcct
     4741 gcggcgtgat gttgaccaat atcgtttcgg gcgggcgcgc gatgtctttg gggatgggga
     4801 gtcctgacaa catggttttt cctgaaaatg taataaaaat attttgcatc cgttccgccg
     4861 cgcagcgcgg gtgcaaaaat atttttcaca atgtctatca tactttaaaa aagaaacttt
     4921 gacacattcc gcacatgccg tttgaacgtg catctgttcc gcacttgcca aacaggggaa
     4981 atgcccctat attgattatc attgcaaaac tttcggaaac ccaatatgca gaccgttacc
     5041 atgtacacag gtccgttttg tccctactgc gcgatggcga aaaggctgct gcacgcggca
     5101 ggtgtcggac atatcgacga aatccgtgtc gatgcaagcc ccgaagcctt tgccgaaatg
     5161 cagcagcttt cgggacagcg cagcgtgccg cagattttca tcggcgaaac gcacgtcggc
     5221 ggatttaccg acctctaccg cctccagcag gaaggcgggc tggacggact gctgaaccct
     5281 taacccccaa ctaggaaaac aaaatgagcg aagaactgca acccgtattc agcatcgagc
     5341 gactgtatgt caaagacttg tctttggaag tgccgcacgc gccgcaaatc tttttggaac
     5401 agggcgagcc cgaagtggat atgcgcgttt ccaccggcag ccaaaagctg gaagacggct
     5461 actacaacgt ggatgtaacc gttaccgtaa ccgccaaatt ggataacgag cgcacgatgt
     5521 ttttgaacga agtaacccaa agcggcatct tccgtttgga aaacatcccc gaagaagatg
     5581 tgcagctgct gctgggcgtg gcgtgtccga acatcctctt cccttacgcg cgcgaagccg
     5641 tttccagtac ggtaacgcgc gccggcttcc cgcccgtcct gctcgcgccg attaattttg
     5701 aggcgattta ccaacaacag caggaagccg aagccgccgg ggcttgattc ctgcctgccg
     5761 atgccgtctg aatccgtttc agacggtatt tttgttttcg gataaaatag gtgcgttcaa
     5821 aggaatctgc ctatgatgtc gcccgaaacc cagaaacagc tcaaaatcac cgatgtttcc
     5881 gccaagaagc tcgacaaact caacctccat accgcgtggg atttggtgtt gcacctgccg
     5941 ctgcgttacg aggacgagac gcacattatg ccgattaagg acgcgccgat tggcgtgccg
     6001 tgccaggtcg agggcgaggt tatccatcag gaagtaacgt tcaaaccgcg caagcagttg
     6061 attgtccaaa tcgccgacgg ttccggcagc gtcctttttc tgcgcttcat ccacttttac
     6121 gccagccatc agaaacagac ggcggtcggc aaacgcatcc gcgccgtggg cgaaatcaaa
     6181 cacggctttt acggcgacga gatgattcat cccaaaatcc gcgatgccga gggcggcggt
     6241 ttggcggaaa gcctcacgcc ggtttacccg accgtaaacg gtttgaacca gcccactttg
     6301 cgccgtatta ttcagacggc gttggacgtt acgccgctgc acgacacgct gcccgatgcc
     6361 ctgctgtgcc gtctgaagct gcaacacctc gccgaaagcc tgcgtctttt gcattcgccg
     6421 ccgccgagtt tcacgattca tcagctttca gacggcacgc tgcctgcatg gcaacggctc
     6481 aaattcgatg aacttttggc gcaacagctt tcgatgcgct tggcgcgaca gaagcgtatc
     6541 ggcggcacgg cggcggcatt gggcggcgac ggcacattga cccaagccct gcgccaagcc
     6601 ctgccgtttg ccctgaccga tgcgcaagaa aaagttgttt ccgaaatctg ccgcgatatg
     6661 gcgcaaacct acccgatgca ccgcctgctg cagggcgatg tcggcagcgg caaaaccatt
     6721 gtggctgctt tgtctgcttt gacggctatc gaatccggtg cgcaggtggc tgtaatggcg
     6781 cccactgaaa tccttgccga acagcatttt attaaattta aacaatggct cgaacctttg
     6841 ggcattgaag ttgtccgcct ttttggcagt ttgcgtaaaa aagccaaaga cgaagccaaa
     6901 gccaaactgg ctgacggcag cgtcaaaatc gccgtcggca cgcacgccct gttttcagac
     6961 ggcgtggcgt ttcacaattt gggcttgacc attgtggacg aacagcaccg tttcggcgtt
     7021 gcccaacgcc tcgccctcaa aaacaaaggg cgcgaagtcc atcagctgat gatgtccgcc
     7081 acgcccatcc cgcgcacgct tgcgatgagt tttttcgccg atttggacgt gtccgtcatc
     7141 gacgaattgc cgcccgggcg cacaccgatt aaaacgcgcc tcgtcaacaa cgtccgccgc
     7201 gccgaagtcg aaggcttcgt cctcggcact tgccgaaaag ggcggcaggc gtattgggtc
     7261 tgcccattga ttgaagaaag cgaaaccctg caactgcaaa ccgccgccga aaccctcgcc
     7321 cggcttcaga cggcattgcc cgaactcaat atcggactgg tacacgggcg catgaaggcc
     7381 gccgaaaagg ccgaagtgat ggcgcggttt tcttcggggg ggctgaacgt cttggtcgcc
     7441 accaccgtca tcgaagtcgg cgtagatgtg cccaatgccg ccctgatggt catcgaacac
     7501 gccgagcgca tgggcttggc gcagcttcac caattacgcg gacgggtagg gcgcggcgcg
     7561 gcggaaagcg tgtgcgtcct cctgtttgcc gaacccttgg gcgaactcgc caaagcgcgg
     7621 ctgaaagtca tctacgaaca caccgacggc ttcgaaatcg cccgccaaga cctcaatatc
     7681 cgcggcccgg gcgagtttct cggcgcgcgc caaagcggcg tgcctatgct gcgtttcgcc
     7741 aaactcgaag aagacctgca ccttttggaa caggcgcgcg aaaccgcccc gatgctgatt
     7801 gagcaaaacc ccgaaatcgt cgaagcgcat ttggcaaggt ggctttccgg cagggaaggg
     7861 tatttgggcg tgtgagcaaa atgccgtctg aaatattaga aaaaaaagtg taacggaaat
     7921 ctg
//
LOCUS       DAWHXZ010000084         7898 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000084 DAWHXZ010000000
VERSION     DAWHXZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.084"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..108
                     /locus_tag="WIE35_001154"
     CDS             <1..108
                     /locus_tag="WIE35_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEZ4154072.1"
                     /translation="VPKKAETISNKLFFTHHPKRQTEFAVRQIRQARAE"
     gene            234..1529
                     /gene="hisS"
                     /locus_tag="WIE35_001155"
     CDS             234..1529
                     /gene="hisS"
                     /locus_tag="WIE35_001155"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ4154073.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            1530..2159
                     /locus_tag="WIE35_001156"
     CDS             1530..2159
                     /locus_tag="WIE35_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ4154074.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFS
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            2313..3770
                     /gene="der"
                     /locus_tag="WIE35_001157"
     CDS             2313..3770
                     /gene="der"
                     /locus_tag="WIE35_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ4154075.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEKEEEEAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            3801..4700
                     /gene="rdgC"
                     /locus_tag="WIE35_001158"
     CDS             3801..4700
                     /gene="rdgC"
                     /locus_tag="WIE35_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ4154076.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLEWFSEGF
                     AAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAASLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            4752..5168
                     /locus_tag="WIE35_001159"
     CDS             4752..5168
                     /locus_tag="WIE35_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154077.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYDGGVGKDRLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNLVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            5234..5800
                     /locus_tag="WIE35_001160"
     CDS             5234..5800
                     /locus_tag="WIE35_001160"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ4154078.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(5864..6568)
                     /locus_tag="WIE35_001161"
     CDS             complement(5864..6568)
                     /locus_tag="WIE35_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ4154079.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEAVQDTMSA
                     RLQVAAEGRDKNAVNAEFVKKFNKFIRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDELNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAILRFK
                     ARADKLSGILGTSGYKIVKLNLGHIGSHIAGEGAAQAKMLRAMPMAASYGVEGTDSAA
                     PGVEEISISVNGTVQF"
     gene            6714..7898
                     /locus_tag="WIE35_001162"
                     /pseudo
     CDS             6714..7898
                     /locus_tag="WIE35_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271049.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         2099 a         2174 c         1897 g         1728 t
ORIGIN      
        1 gtgccgaaaa aagccgagac aatttcaaat aaattgtttt tcacacatca tcccaagcgg
       61 cagacagagt ttgcagtccg acaaatcagg caagcacgtg cagagtaagt caaatgccgt
      121 ctgaaaactg tttcagacgg cattttaccc gtccgcaaac cacccccaac ctcacaaacc
      181 cttaaaaatc cgctataatc gcccgcaatt tgatttaagc attcttactt gaaatggcac
      241 aaaaaatcca atccgtcaaa ggcatgaacg accttctgcc tgtcgagcaa aaagatttca
      301 aactgacggc tgcgttttgg caggcgtttg aggatacggt aaaccgctgg acgcgcgctt
      361 acggctatca gcaaatccgt acgccgattg tcgagcaaac cggtttgttt gtccgctcca
      421 tcggcgagga aaccgatgtg gtcggcaagg aaatgtacac cttttccgat tcaaacgatt
      481 ctttgagttt gagcctgcgt ccggaaggta cggcttcttg tctgcgtgcg gtggtcgaac
      541 acaaccttct gtacaacagc ccgcaaaagc tgtggtatat ggggccgatg ttccgccgcg
      601 agcgtccgca aaaaggccgt tatcgtcaat tccatcaggt cggtatcgag gctttgggtt
      661 ttgaagggcc ggatatcgat gcggaaatca tcgcgatgtc tgccgacttg tgggaaaaat
      721 tgggtatccg cgaatacctg actttggaaa tcaacagctt aggcaaccgt gaggagcgag
      781 cggcacaccg tgcggcgttg gttgaatatt tgacacgcta tgaagataaa ttggacgaag
      841 acagcaaacg ccgtctgaaa accaatcctt tgcgtgtttt agatacgaaa aaccctgatt
      901 tgcaggaaat ctgcaacgcc gcgccgcgtt tggtggatta tttgggcgag gcttcgcaaa
      961 accactatgc acgcttcaag gcgatgttgg acggtttggg tatccaatat attgaaaatc
     1021 cgcgcttggt tcgcggtttg gattattaca atcagacggt ttttgagtgg acgaccgaca
     1081 aactcggcgc gcaggcaact gtgtgcggcg gcggccgtta cgatggtttg attgaggaac
     1141 ttggcggcaa acctgcgccg tccatcggct ttgcgatggg catcgaacgg ctgctgcttt
     1201 tggtgagcga atacggctcg ctggaagtga acgctgcgcc tgatgtctat gcaatgcacc
     1261 aaggcgaaag ggcggacttg caggtgatga aatacgcgca agccttgcgc gcgcaaggtt
     1321 tcaatgtaat gcagcattcc ggctatcaaa gcctgaaagc gcaaatgaaa aaagccgaca
     1381 acagcggcgc gcgctttgcc ctgattgtcg cgcaagacga actggcgaac ggtacggtta
     1441 cgctcaaaga catgaacggc gcacacggtc agcaaactgt cgccgccgag gatttaaccc
     1501 atactttaca acaatggaag aacgcataaa tggcagccca tctcgaagaa caacaagagt
     1561 tagacaactt taaatatttt tggaaaacca cgggcaaatg gctgtttgcc ctgctgattt
     1621 tggcagcact cggctacttg ggatacacgg tttaccaaaa ccgtgcggct tcccaaaatc
     1681 aggaagcggc ggcggtgctg gcaaacatcg tggaaaaggc gcaaaacaaa gccccgcaaa
     1741 gcgaaattaa tgccgaattg accaaactcc aacaaagcta cccgcattct atttccgccg
     1801 cccaagccac gctgatggcg gcggcaaccg aatttgacgc gcagcgttac gatgttgccg
     1861 aaggccattt gaaatgggtg ttgtccaacc aaaaagacag cctgattcaa gcgttggcgg
     1921 cgcagcgtct gggcgttgtg ttgttgcaac aaaaaaaata cgatgccgcg cttgccgcgc
     1981 tcgacacgcc ggttgaagcg gacttctccc ccctgctgat ggaaaccaaa ggcgatgtct
     2041 atgccgcaca gggaaaaagc caggaagcct taaaaaacta cggtcaggct ttagaaaaaa
     2101 tgcctcaaga ttctgtcggt cgtgaattgg ttcaaatgaa actcgattcg ctgaaataaa
     2161 tgccgtctga aatgccgtcc attatttatc agacggcatt ttaccctccc tctttatggc
     2221 ctcttgccgt gaagaaccta tttaccgtgt ccaacggaca cctgctcgtg ttcagttctc
     2281 ccgaacggca cttgacacag aaagactcaa tcatgaaacc aaccatcgcg cttgtcggcc
     2341 gccccaacgt cggcaaatct actttattca accgtttgac gcgcaccaaa gacgcactcg
     2401 tacacgacct gcccggcctg acccgcgacc gccattacgg acacggcaaa gtcggcagca
     2461 aaccttattt ggtcatcgat accggcggtt tcgagccggt tgtggacagc ggcattttgc
     2521 acgaaatggc aaaacaaacc ttacaggctg tcgatgaagc cgatgcggtt gtgtttttgg
     2581 tggacggccg taccggtctg acgccgcaag ataaaatcat tgccgaccgt ttgcgccaaa
     2641 gcccgcgccc cgtttatttg gccgtgaata aaggagaagg cggcaataga gccgtgcttg
     2701 ccgccgagtt ctacgaactt gctttgggcg acccttatgt tatttcaggt gcgcacggtg
     2761 acggcgtgta ttatctgatt gaagatattt tggaaacctt ccctgagcct gaaaaagaag
     2821 aggaagaggc gaaacatcct gtttttgccg ttatcggtcg tccaaatgtc ggtaaatcaa
     2881 ccttggtaaa cgccattctc ggcgaagagc gcgtcatcgc cttcgatatg gcaggtacga
     2941 cgcgcgacag tatccatatc gatttcgagc gcgaaggcaa accgtttacc atcatcgata
     3001 ccgcaggtgt gcgccgtcgc ggcaaagtgg atgaagcagt ggaaaaattc tccgttatca
     3061 aagcgatgca ggcggttgaa gcggcaaacg tcgctgtttt ggtattggac gcgcagcagg
     3121 acatcgccga ccaagatgcg acgattgcag gtttcgcttt ggaggcaggc cgtgctttgg
     3181 tggtggcggt caataaatgg gacggcatca gcgaagaacg tcgcgagcaa gtgaaacgcg
     3241 atatcaaccg caaactgtat ttcctcgatt ttgccaagtt ccactttatt tccgcattga
     3301 aagagcgcgg tatagacggt ttgtttgaca gcattcaggc tgcctacaac gcggcgatga
     3361 ttaagatgcc gacgccgaaa atcacgcgcg tattacaaag cgcgatcgag cgtcagcaac
     3421 cgccgcgtgc cggcttggtg cgtccgaaaa tgcgttatgc ccaccaaggc ggcatgaacc
     3481 ctcccgtaat tgtggtacac ggcaattcgc tgcacgcgat ttccgacagc tatacgcgct
     3541 atctgaccca aacgttccgc aaagccttca atctgcaagg cacgccgctc agaattcaat
     3601 acaatgtttc ggaaaacccg tatgaaaatg cggaagacaa accgaagaaa aaaccgctgc
     3661 gccgcgtcag cctgagcaac cgtattgaga aacgcgaagg ccgcaaagaa gagaaaaacc
     3721 gcttcaagaa gaaaaccaaa gtcagtgtga aaaaacaatt cagcaaataa ttcccgacat
     3781 ttcaaacaat aggaaacatt atgtggttca agcagattag tttttatccg ctcaacaaag
     3841 aaaagctgcc tgaggcagac gtacttgccg acaaacttgc tgaagctgaa tttacccatt
     3901 gccaaggctt agagtggttc agcgaaggct ttgccgcacc ggtttcgttc tctcccgaac
     3961 tcgttttccc tgccgacttt accttgcgcg tcgccctgaa aaaagaagaa aaagtcctgc
     4021 ctgccggcgt catccgcgat attttggaag agaaggtggc ggaaatccaa aacaatgaag
     4081 cccgcaatgt cggtcgcaaa gaaaaacaag agcttaaaga gcaaattaca gacgacctgc
     4141 tgccccgagc gtttacccgc agcagccgta cagaagcggt gtttaacacc cgccacggct
     4201 acctgctcgt caataacgcg gcttccgcca aagcagaaaa catcctgacc aagctgcgcg
     4261 aagctttggg cggtttggaa gcctcgctgc cgaataccaa acaatcgccc tcttctctga
     4321 tgaccggctg gctgttgcaa gggcattgcg aaggcggttt tgaattggac agcgattgcg
     4381 aactcaaagg tacgggcgat attgttcccg tcgtcaaagt atccaaacaa gatttaaccg
     4441 ccgacgaagt ggttcaacac gtcaaaaacg gtaaaaccgt aacccagctc ggtttggttt
     4501 ggcgcgaaca aatcgccttt atcctcactc aagacttcac actcaagcgc atccaatacc
     4561 tcgacgtatt gcaggaagaa gccgaaagca acggcgacga tgccgccagc cttgccttcg
     4621 cctcgcaaat cctgatggct gaatccgtca gcaccatgtt ggaagagctg gtttcctatt
     4681 tgggcggctg gcaagattga tttttcagac ggcttttata ccgtcagcca aaatccaatg
     4741 gaataaccct tatgcttaaa catctcgcat tcctactgcc cgccatgatg ttcgccctcc
     4801 ccgccgcgtc cgccgttctg acttcctatc aagaacccgg ctgcacctac gacggcggag
     4861 tcggaaaaga caggcttcct tcaggcaaag gcatatggcg ttgccgggac gggcgcggtt
     4921 ataccggttc gtttaaaaat ggcaaattcg acggacaagg cgtttatacc gttgccgcca
     4981 accgcgaaat atttatcgaa ccgttcaatt ccgacagtac caaattccgc aacttggtac
     5041 tctcgggcac gttcaaaaaa ggcttggcac acggcagatt taccgtctcg caaaacggcg
     5101 aaaccctctt cattatgaaa tgcgaaaacg gcatgattaa agaagtgaag ctgcccaaaa
     5161 acaaataagc cccgttttca gacggcatcc cccaatgctt tgtccgaaca ctgcaagaaa
     5221 caaggaaaac attatgagca tcaagtccga caaatggata cgccgcatga gcgaagaatt
     5281 cggcatgatc gaccctttcg agccgaacca aatcaaagaa gccgacggca aacgcatcat
     5341 ctcctacggt acgtccagct acggctacga catccgctgc gccaacgaat ttaaaatctt
     5401 caccaacatc aacagcacca tcgtcgatcc caaaaacttc gacccaaaaa acttcgttac
     5461 cgttgaagac gactgctgca tcatcccgcc taattccttc gcactggcgc gcacggtcga
     5521 atatttccgc atcccgcgca acgtcctgac cgtctgcttg ggcaaatcca cctacgcccg
     5581 ctgcggcatc atcgtcaacg ttaccccgtt cgaaccggaa tgggaaggct acgttaccct
     5641 cgaattttcc aacaccaccc ccctgcccgc caaaatctac gccggcgaag gcgtggcgca
     5701 agtcctcttc ttcgagagcg acgaaatctg cgaaacctca tacaaagacc gcaacggcaa
     5761 atacatggga caaaccggcg taaccctgcc caaagcctga aatataaatg ccgtctgaac
     5821 ccttatcgtc gggtttcaga cggcatttgc cgttcacctc aaatcagaac tgaaccgtcc
     5881 cattgacgct gatgctgatt tcctccacac ccggcgcggc ggaatccgta ccctccacgc
     5941 cgtaacttgc cgccatcggc atggcacgaa gcatttttgc ctgagcagct ccttcacccg
     6001 cgatatggct gccgatgtgt cccaaattta atttgacgat tttataaccg gacgtaccca
     6061 atatgcccga caacttgtct gcacgcgcct tgaaacgcaa aatagcatct ttactgacct
     6121 gatcgatgac ctcgtttcgg cgttcgcgcg acacatggaa atccgtatat tccaatgcgg
     6181 catctgcctg aacatcggca ataaaacggt ttaactcatc aaaatccctc ccttcgacct
     6241 taaattccgc acgctcctcc caacctgttt gaatgcgtct gccgttggta tattgatagc
     6301 gcggcatcgc actgcgcgat accaattcgg ttttaaagct accatttttc gattttctga
     6361 tgaacttgtt gaatttttta acaaactcgg cattgacggc atttttgtcc cgtccttccg
     6421 ccgccacctg taaacgcgcg gacattgtat cctgaaccgc ctcgacaccg gccgattcgg
     6481 aaaattcgac aatattgtaa ttcaatgctt cagccgctgc cggaaaagat accgccaata
     6541 gggaagccgc caaaatagaa cgcagcatat ccgtatcctt acttataaaa ccgccaatct
     6601 agcatatcgg cgcgctaaat tgcgttagcg taaggcaaca atttaaatat tcggtctgcg
     6661 tctgtcttat aataaccgcc tttccgatac ggaatcccac tatgcccaaa cccttgcacg
     6721 tcctcgtcat cccctcatgg tatccgcaat ccgaacagga tgtggacggg atttttttca
     6781 aaatcaggca ctggcattgc agaggaaagg catcaaaacc gccgtgcttg caccgatgtt
     6841 ccgctacttg cggaaagaaa cagcaagcat cctgaccgac cttacggttt tgcaaaatat
     6901 cggcaaagcg gtttggacat ctatgcatgg cacggcatgt atttcttccc ccgctttccg
     6961 ttcatcgaca tcgaccgcat ccgctgggtg cgcgtgggtt tgaaagcctt caaacactac
     7021 atccgcgaaa acgggctgcc cgacctgatt cacgcccacc gcatgaacta tgccggcata
     7081 ctcgcccaaa aaatttccca aaaatacggc atcccctatg tcgtcacgaa acacagcagc
     7141 accattacgc gcggtctgat ccgtccccac caatggcagc ctatgaaaaa tgcggcggcg
     7201 cacgccgccg cacgtctcgc catcagccgc catttcgcac acgtcctgca acacaaatac
     7261 ggctgcgaat ggcagtacct ccccaacata ctgggcggaa tattcaaccg accgtttgaa
     7321 caaagagaaa aaaacaacaa accgcatttc gtgttctgca ccgtctcgca ccttcgccgt
     7381 atcaaaggac acgatgtcct gctcactgcc tttgcccggg cgttggcaca atgcccgcaa
     7441 ctgcgtctaa acatcggcgg cagcggacag gaagaacagc ggctgaaaca gcaggcggca
     7501 gacttgggca ttacccatgc cgttacattt ttgggcgcac tgcaacccga agcagtcttg
     7561 gatttgatga agaacagcga cgcatttgtc cttgccagcc gcaccgaaac cttcggcgtg
     7621 gtctatatcg aagcactgtc ccaaggattg cccgtcattg caacacgctg cggcggtgcg
     7681 gaatctattg tttcagacgg caacggatat ttggttcctg ttgacgacga cgatgccctt
     7741 gccgacgcac tcatcaaaat gtatgaacac cactctgatt tcgaacctgc ccgacttagg
     7801 gaaaactgtc tgaacgaatt tggcgaaaat gccgttatag gcaggttgat cggcattttc
     7861 cgacaggcaa tcgcagaata cggtaagaaa ataccggt
//
LOCUS       DAWHXZ010000085         7890 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000085 DAWHXZ010000000
VERSION     DAWHXZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.085"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            112..222
                     /locus_tag="WIE35_001163"
                     /pseudo
     CDS             112..222
                     /locus_tag="WIE35_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            341..745
                     /locus_tag="WIE35_001164"
     CDS             341..745
                     /locus_tag="WIE35_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ4154080.1"
                     /translation="MGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAG
                     HGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGW
                     LLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(780..1655)
                     /gene="rsmI"
                     /locus_tag="WIE35_001165"
     CDS             complement(780..1655)
                     /gene="rsmI"
                     /locus_tag="WIE35_001165"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ4154081.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGQLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMVELFPERRLMLAREITKTFETFLSGTVGEIQT
                     VLSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            1701..2048
                     /locus_tag="WIE35_001166"
     CDS             1701..2048
                     /locus_tag="WIE35_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ4154082.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            2053..2646
                     /locus_tag="WIE35_001167"
     CDS             2053..2646
                     /locus_tag="WIE35_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ4154083.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            2710..3318
                     /locus_tag="WIE35_001168"
     CDS             2710..3318
                     /locus_tag="WIE35_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ4154084.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3326..3994
                     /locus_tag="WIE35_001169"
     CDS             3326..3994
                     /locus_tag="WIE35_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154085.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKEKETPKENHTKPDTPKNTPPKPHKEIL
                     DNLF"
     gene            4034..4813
                     /gene="map"
                     /locus_tag="WIE35_001170"
     CDS             4034..4813
                     /gene="map"
                     /locus_tag="WIE35_001170"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ4154086.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4968..5267
                     /locus_tag="WIE35_001171"
     CDS             4968..5267
                     /locus_tag="WIE35_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ4154087.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5505..5879
                     /locus_tag="WIE35_001172"
     CDS             5505..5879
                     /locus_tag="WIE35_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ4154088.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(6066..7532)
                     /locus_tag="WIE35_001173"
     CDS             complement(6066..7532)
                     /locus_tag="WIE35_001173"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ4154089.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         2054 a         2351 c         1914 g         1571 t
ORIGIN      
        1 agttcaatga gtttattttg tttataccgg cttagacgac tttttctcat agggataatt
       61 ctaacttaat ttgaatttcc ctagttatct aggacagccc ctaagttttt tatgaacaca
      121 aaccaacctg ccgtttacga cccgttgaca cgtacgctgc actggctgac cgttgccggt
      181 ttcatcggca ttccgaccac cattgtcctg tggacgattt atagtggatt aacaaaaatc
      241 aggacaaggc gacgaagccg cagacagtac aaatagtacg gcaaggcgag gcaacgccgt
      301 actggttttt gttaatccac tatacgaaga ggcggaatgg gtgggcagcc tgttcggcct
      361 gcacaaatct ttcggtttcc ttacgctgac ggtgattaca ttgcgcatcg tgtgggcggt
      421 tgccaaccgc gccaagcgtc cgcaaagcga cagcaaggct gcggcggcag gacacggcat
      481 tctgtatctg ctcatgcttg ccgttcccgt tatcggcatg atccgccaat acggcagcgg
      541 ccgcggcccg ttgaaagtgt tcggcgttga agtgatgcag ggttcgccgg aaaaaatcga
      601 gtggatggca aacttgggca acacgttcca cggcaatttg ggctggctgc tgtttgccgc
      661 cgtcgtcgga cacgtcgcca tggtcgtcgt ccaccgtgtt caaggcaaag acgtgctgta
      721 ccgcatgacg gggcgtgtcc gttgattccg ttcgcactat ggtgccggtc cgtccggcat
      781 catttgtttt tccaagacag tgccagatcg tacaaagctt tttttccctc gcccgtgatt
      841 ttggcggcaa gctccgccgc ctgtttggtc ggcagctcgg ctgtgaggat tttcatgatg
      901 ttttgcgcgg actcggacaa gccttcgtgt ttttcatcct gcgccggata aagcaccaac
      961 accatctcgc cgcgcgattg gttgccgtcg gcagacaata ccgtctgaat ttccccaacc
     1021 gtgccgctta agaacgtttc aaacgttttc gtaatttcgc gcgccagcat taatcggcgt
     1081 tcggggaaca attccaccat atcggcaagc gttgccccga ttcggtgtgg cgtttcaaac
     1141 atgacgatag gaaatgccgc ccgcacccat ttggcaaaca atttcctacg ttcgcccgat
     1201 ttcggcggta caaaaccgtt gaaataaaaa tcggatcccg ccacaccagc cacactcaaa
     1261 gccgccatca ccgcgcttgc gccgacaaca gggacaactt taaacccgac ctcacgcacg
     1321 cggcgggcga gtttcgcgcc cgggtcgcac acggccggcg tacccgcatc ggaaacctgt
     1381 gccacaacca tgccgtctga aagatagccg acaatcttgt ccgccatctg ccgttcgttg
     1441 tgttcgcgca cgctgacgag ttggccctga atgccgtacg cgctcaaaag ctgcgcggta
     1501 acgcgcgtat cttcggcaca gatgatgtcc gccttttgca ataccgccaa agcgcgcagg
     1561 gtaatgtccg ccaaattgcc gatgggcgtg gcaaccacgt ataatgtccc tccgacgacg
     1621 ctgtcggagg ctttctgcaa atgtttctga aacataagaa tgccgtctga aaaacaaaca
     1681 ttataaaggt taaaccgatt atgcgcctaa accacaaaca gggcgaggca ggggaagatg
     1741 ccgcgcttgc cttcctccaa tcccaaggct gcacactgct tgcccgcaac tggcactgcg
     1801 cctacggcga aatcgacctg attgtcaaaa acggcggcat gattctgttt gttgaagtaa
     1861 aataccgcaa aaatcggcaa ttcggcggtg ccgcatacag catttcccca tccaaattat
     1921 tgaaactgca acgaagtgta gagtattatc tgcaacagaa caggttgaca aacgtaccgt
     1981 gccgcctcga cgcggtactc atcgaaggca gccgcccgcc cgagtggata cagaatatta
     2041 caggttgacg atatgacgac attacaagaa cgcgttgccg cccattttgc cgaaagcatc
     2101 cgtgccaagc aggaagccgg aaaagtattg gtcgagccga ccgtacaggc tgccgagctg
     2161 atgctgcaat gcctgatgaa tgacggcaaa atcctggcct gcggcaacgg cggttcggct
     2221 gccgacgcgc aacacttcgc cgccgaaatg accggccgtt ttgaaaaaga acgcatggaa
     2281 ctcgccgctg tcgcgctgac aacagacact tccgcgctga cagccatcgg caacgactac
     2341 ggtttcgacc acgtattcag caaacaggtg cgcgcgctcg gacgtgcagg cgatgtattg
     2401 gtcggcattt ccacctccgg caattccgcc aacgtcatcg aagccgtcaa agccgcacac
     2461 gaacgcgata tgcacgtcat cgccttgacc ggccgcgacg gcggcaaaat cgccgccata
     2521 ctcaaagaca ccgacgtttt gctcaacgtt ccccatccgc gcaccgcccg tattcaagaa
     2581 aaccacatcc tgctgataca cgccatgtgc gactgtatcg actccgtact gctggaagga
     2641 atgtaaccct tttcagacgg catggcgcaa agcaatgccg tctgaaacgc ccaagaaagg
     2701 aagcacccga tgaaacccaa accgcacacc gtccgcaccc tgattgccgc cattttcagc
     2761 cttgccctta gcggctgcgt cagcgcagta atcggaagcg ccgccgtcgg cgcgaaatcc
     2821 gccgtcgacc gccgaaccac cggcgcgcaa accgacgaca acgttatggc gttgcgtatc
     2881 gaaaccaccg cccgctccta tctgcgccaa aacaaccaaa ccaaaggcta cacgccccaa
     2941 atctccgttg tcggctacaa ccgccacctg ctgctgctcg gacaagtcgc caccgaaggc
     3001 gagaaacagt tcgtcggtca gattgcacgt tccgaacagg ccgccgaagg cgtatacaac
     3061 tacattaccg tcgcctccct gccgcgcact gccggcgaca tcgccggcga cacttggaac
     3121 acatccaaag tccgcgccac gctgttgggc atcagccccg ccacacaggc gcgcgtcaaa
     3181 atcgttacct acggcaacgt aacctacgtt atgggcatcc tcacccccga agaacaggcg
     3241 cagattaccc aaaaagtcag caccaccgtc ggcgtacaaa aagtcatcac cctctaccaa
     3301 aactacgtcc aacgctgact cggcaatgcc gtctgaaccg ccttcagacg gcattgcccg
     3361 acaccccaaa agcacaatca aaatggcaaa aaaaccgaac aaacccttca ggctgacccc
     3421 caaactcctg atacgtgtcg tattgctcat ctgtatcacc gccatcggcg cattggcaat
     3481 aggcatcgtc agcacattca acccgaacgg cggcaaaacc ctccaaaccg aaccgcaaca
     3541 caccgacagc ccccgcgaaa ccgaattctg gctgccaaac ggcgtagtcg gacaagatgc
     3601 cgcccaaccc gaacaccacc acgccgcctc atccgaaccc gcacagccgg acggcacaga
     3661 cgaaagcggc agcggactgc cgtcccctgc cgcacccaag aaaaaccggg tcaaaccgca
     3721 acctgccgac acagctcaaa ccgacaggca gccggacgac gccggaacac aagctgaaaa
     3781 cacactcaaa gaaacccccg tactgcccac aaacgtcccc cgtcccgaac cccgaaaaga
     3841 aacacccgaa aaacaggcgc agcccaaaga aacacccaaa gaaaaagaaa cgcccaaaga
     3901 aaaagaaacg cccaaagaaa accataccaa accggacacc ccgaaaaaca cgccgcccaa
     3961 accccataaa gaaattctcg acaacctctt ctgacccggc acggcaggca cacccgcaat
     4021 ccaaggaagc attatgaacg gcatcatcat caaaaccccc gaagaaatcg aaaaaatgcg
     4081 cgagctgggc aaactcgtcg ccgaagccct cgactacatc ggacaattcg tcaaacccgg
     4141 cgtaaccacc gacgaaatcg acaaactcgt ttacgactac cacgtcaacg tccaaggcgg
     4201 ctatcccgcc cccctgcact acggcaaccc gccctacccc aaatcctgct gcacctccgt
     4261 caaccacgtc atctgccacg gcattcccga cgacaagccg ctcaaagaag gcgacattat
     4321 caacatcgac ctcaccatca aaaaagacgg cttccacggc gactccagcc gtatgtttac
     4381 cgtcggcaaa gtctccccca tcgcccaacg cctgatcgac gtaacccacg cctccatgat
     4441 ggcgggcata gaagccgtca aacccggcgc gacactgggc gacgtaggtt acgcctgcca
     4501 acaggttgcc gaaaacgccg gctattccgt cgtacaggaa ttctgcggac acggcatcgg
     4561 gcgcggtttc cacgaagccc cgcaagtgtt gcactacgga aaaaaaggac agggccccgt
     4621 tctaaaaccg ggtatgattt ttaccgtcga accgatgatc aaccaaggca aacgccacct
     4681 gcgtatcctc aacgacggct ggacggtggt taccaaagac cgctccctct ccgcccaatg
     4741 ggaacacgaa gtcttggtga ccgaaaccgg ctacgaaatc ctcaccgtca gccccgcctc
     4801 cggcaaaccc tgaaaccgga cgtatccgcc ccataaaaac aaacaatgcc gtctgaaaga
     4861 aacggcagat atgatatata atataaaaac aggcttgacc cggcacatta cgaaaacaaa
     4921 gcaaatcgga atttgccccg caaccagaca aacttaaagg aagttttatg aaaatatttg
     4981 aaaatataga agatgttaaa gccatccgta aaaagaccgg gctgaaccag atagacttct
     5041 ggggcaaggt cggcgttacc cagtctggag gctcgcgcta cgaaaccggc cgcaaaatgc
     5101 ccaaacccgt acgcgaactg ctccgcctcg tccatatcga atgcatcgat ttggcgaaag
     5161 tcaacaaaaa agatatggaa atcgccgccc tgttgaaaaa acaccatccc gacctgtatg
     5221 ccgaattgtc caaacagacc aagtccgaaa gaaaaaaaca aagttaaacc gcaacctccg
     5281 gatgcccgaa ggtttttcat ttccgaaaaa cgcaaacaat gccgtctgaa acaccggaca
     5341 ggccgccgta tcccgcctgc cgcccctgcc tcaaaccgcc gaaccgcccg aacccgcctt
     5401 tttacaaact ttatccaatt tcctgtttat ttcaggatac gccgacatta gaatgtcaaa
     5461 cagctcgaaa cgggcaaact ccacatccat ccaaaggaat aaaaatgaaa cttctgacca
     5521 ccgcaatcct gtcttccgca atcgcgctca gcagtatggc tgctgctgcc ggcacgaaca
     5581 accccaccgt tgccaaaaaa accgtcagct acgtctgcca gcaaggtaaa aaagtcaaag
     5641 taacctacgg cttcaacaaa cagggcctga ccacatacgc ttccgccgtc atcaacggca
     5701 aacgcgtgca aatgcctgtc aatttggaca aatccgacaa tgtggaaaca ttctacggca
     5761 aagaaggcgg ttatgttttg ggtaccggcg tgatggatgg taaatcctac cgcaaacagc
     5821 ccattatgat taccgcacct gacaaccaaa tcgtcttcaa agactgttcc ccacgttaat
     5881 caggcaacaa aaaacagcgt tttcagaaat gaaaacgctg ttttttgact gttccattat
     5941 tcacaaaagg gaaaaaacga ttacctgccc cgtgtaccaa aacctgccct gccggatgaa
     6001 gggcataacc ggcagggacg gcatcaacac catatggggg tacggctttt cttgaaagat
     6061 tcggcttaaa tatccaacac tttcgcggta taggcgataa tttcatccgc cctttcaggg
     6121 ttttcgttca acttgatgcc gtaacccggt accagctctt tcagacgacc ttcccaagac
     6181 ggggcgcgct cggggaagca ttggtgcatc agccggatca tcagcggcac agcggtcgat
     6241 gcgcccggcg acgcgcccag caatgcggcg agtgagccgt cggcgtgggc gacaatctcc
     6301 gtaccaaact ggagcacgcc gcctttttcg gagtcttttt taatgatttg gacgcgttgc
     6361 cctgcggtga tgagttccca gtcgtcgggg tttgcctcgg ggtagtattc cagcagggag
     6421 gcgaagcgtt cttctttggt tttacgcaat tcgcccagca ggtatttggt cagcggcata
     6481 ttcgcccagc cggcgcgcag cataggatag agattgtcca tatggatgga cagcggcaaa
     6541 tccataagcg agccttgctt gaggaagttg gaacggaagc ctgcgtaagg gccgaacata
     6601 aggtggcgtt tgccgtccac gttgcgtgtg tcgaggtgcg ggacggacat cggcggcgcg
     6661 ccgacggaag cctgcccgta cactttggcg ttgtgttgtt cggcggtttc ggggttgctg
     6721 ttgcggaaga acaggccgga cacggggaag ccgccgtagc ctttgccttc ggggatgccg
     6781 gatttttgca gcagggtcag cgcgccgccg cccgcgccga ggaagaggaa gcgggtacgg
     6841 agggtgagct gcccgtcggg gttgcgggta tcggcggttt tgagcaccca cgcgccgtcg
     6901 gattcgcgtt tgatgtcttc gacgtggcgg ttgaactcgg tttttacgcc cttgccctgc
     6961 aaatatttca ccatttggcg cgtcagccgt ccgaaatcga catccgtgcc ttcggcggag
     7021 tagttggcgg cgacgggttg gttttcgtcc cggccgcgca tcatcagcgg agcccaatcg
     7081 gaaattttgt tccgatcggt ggaaaattcc atattttcaa aaagtttttg ggttttaaac
     7141 gcgtcataac gtttttgaag ataagaacaa tggtcttcat tcatcaccaa agacatatgc
     7201 ggcacggcat tgatgaagga attgtcttcc aacttgcctt ccgcgaccag cgtcgcccaa
     7261 aactggcggc tgacatgaaa ctgttcggca atattgaggg cgcgcgccgg atcgataatc
     7321 ccatttgcac ccaacggcgc atagttcaat tcgcacagcg cggaatgccc cgtgccggcg
     7381 ttgttccacg cgtttgacga ttccaacgcc acatcttcca agcgttcaat cagggtgatt
     7441 tcccaagacg gttcgagttc tttgagcaaa acgcccaaag tcgcgctcat aatgccgccg
     7501 cccaccaaga caacgtctgt cgcttcagcc atggtttact cctaaaaaac aggcatcttc
     7561 tgcccttatg gttatttgcc gtactacaaa cgcctgaatc gcaaaagcag ggaaaaccgg
     7621 caatggtgtg tgtccgagta tgctgtttcg gggttggaat gcgttgcaag catggcttcc
     7681 gacaccgctt caggggcttg taatatgtta tcgtgaatgt agtggatttt actgggaaat
     7741 gcaaagtttt tctgtcgccc gccaagtcgg gaaactgcga aatgaaaaat aaaaatagtt
     7801 atttatctat atatatcaaa tttttaatag ataaaaaatc aaaattgttt atatattaat
     7861 ttttaaaaga ttgtcagcat attgcgttaa
//
LOCUS       DAWHXZ010000086         7848 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000086 DAWHXZ010000000
VERSION     DAWHXZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.086"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(90..1886)
                     /locus_tag="WIE35_001174"
     CDS             complement(90..1886)
                     /locus_tag="WIE35_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ4154090.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGTNPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2344..3597
                     /locus_tag="WIE35_001175"
     CDS             2344..3597
                     /locus_tag="WIE35_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ4154091.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCIISATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3752..5263
                     /gene="lysS"
                     /locus_tag="WIE35_001176"
     CDS             3752..5263
                     /gene="lysS"
                     /locus_tag="WIE35_001176"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ4154092.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHTQYGEIGKEELDPQGIPVKIAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEILDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANGESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5340..5528)
                     /locus_tag="WIE35_001177"
     CDS             complement(5340..5528)
                     /locus_tag="WIE35_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ4154093.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLTALSEWVGQLESE"
     gene            complement(5532..6410)
                     /locus_tag="WIE35_001178"
     CDS             complement(5532..6410)
                     /locus_tag="WIE35_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ4154094.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNHSDADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLVVVEPRMRPA
                     DEDIVLIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEAFDLPEGSTILGVVLEVKKRF
                     MSAAQARRRVDSDYHP"
     gene            complement(6440..7567)
                     /locus_tag="WIE35_001179"
     CDS             complement(6440..7567)
                     /locus_tag="WIE35_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ4154095.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
BASE COUNT         1705 a         1988 c         2200 g         1955 t
ORIGIN      
        1 cttgcggcaa gatgaaaacg gtgcgggatg ttttgggaaa ccaatgccgt ctgaagggct
       61 ttcagacggc attttttgcg ccgtgctgtt taacgcgcca gcggttcggt gcgtttgatc
      121 agccacgctt ttgccgcgcc ttcggtcagc ggctcgaggc ggcggcggac ttcggcgtgg
      181 tagcggttga cccagtcgat ttcgccgtcg gtcatgaggg cggtgtccat caggcgggtg
      241 tcgatggggc agagggtcag ggtttcaaaa cagaggaagc tgccgaattc ggtttcttga
      301 ggggcggcga cggcttggtt ggcggcaagg ttttcaatgc ggatgcccca ttttcccggg
      361 cggtagagtc cgggttcgat ggaggtaacc atgccttttt tcatggcggt ttcgggcgtg
      421 gcgggggcgg cgaaggcgat gcgctgcggg ccttcgtgga cgttgaggaa atagcctacg
      481 ccgtgtccgg tgccgtggcc gtagtcgcat tgcgcctgcc acaggggttt gcggcaaatc
      541 gcatcaatca gcggcgaggg gatgttttct gggaacacgg cttcggcaag cgcgatatgg
      601 gctttgagaa cgagggtgtt gtcgcgtttt tgttcggcgg tgggtgtgcc gacagggacg
      661 acgcgggtga tgtcggtcgt gccgcctttg tattgcgcgc cggagtcgat gagcagcagc
      721 ccgttgccgc tgatggtgct gtggctttcg ggcgttgcgc tgtaatgcgg cagggcgccg
      781 ttggcgttga agcctgcgat ggtgtcgaaa ctcaatgaca caaaacccgg gcgcacgctg
      841 cggtggcggt aaagcatggt gtccacgtcg atttcggtca ggctgccgcc gttgccgatg
      901 atgtcttcaa actcggcgaa gaaaccgcac aacgccgcgc cgtcgtgttc catcgcttcg
      961 cggatgcggg cgatgtcggc ttcggatttg caggatttga acagcgtgga cgggttggtg
     1021 ccttcgatta ggcgtacgct ttcgggcagg cgcacgagcg tactgacggc ggttttgttc
     1081 ggttcgataa gcagcgcgcc gccgatttgc gcgagcttgt cggcaacttg ggcgtaaggt
     1141 tcgaccgtga tgccggcggt ttgcagcgcg gcggcggctt cggcgttcag acggcattgt
     1201 tcggtaaaca ggacggcgtt gtctttgcca atcagcagga aggacacgaa aacgggattg
     1261 aaaggcacgt cgctgccgcg caggttggtc agccaagcga tgtcgtcaag cgatgaaacc
     1321 aagtggtaat ccgcgccttt ttccgccatc acggcgcgca cgcgggcgag tttttcggcg
     1381 gcggtttcgg agacgtaggc ggggtcgtgg atgaacacgg tttcggcggg gatagcggga
     1441 cggcttgtcc acacttggtc aagcagatcg tccgggtgtt cgatgcggat gtttttggcg
     1501 gtcagggatt gcgccaaagt gcgtttgccg gtgagcgaga ccatatcgga agggatgccg
     1561 acggcggcgt tttcgggcag gtttgccgcg agccattcgt tatacggcgg cacttgcccg
     1621 cttttttgca gttcgatgcc gctgcccgca agctgcttgg cggcttgttc ccaatagcgg
     1681 ctgtccaccc atacgcccgc ttcatcggtg gtcaggacaa acgtgccgac cgagccggta
     1741 aagcccgata attcgcggcg cgcctgccaa tgctcgggca ggtattcgga caggtggggg
     1801 tcggcggaag ggatgacgag cgcgtccaag ccttgcgcct tcatggcttc gcgcaatgcg
     1861 gacaggtaat tcgatacggt attcataaca gttcctccaa tcgggttttg cggcttcaga
     1921 cggcatggac ggaaatctga aatgccgtct gaaaaacata accatcttat caaatcgcca
     1981 gcctgctgca aaacggacgg gctgatcgga tggtgcgtgt ttgcgcgaaa aaagcgatgt
     2041 aaggttgttt gttgtaaaat tttaggcata aaatgtcgtt ttatgccttt ttttggaagt
     2101 aatatggaaa atatagtcaa gtttgagcat gaagttctaa tagtgtgtgt tggcaggtat
     2161 ttggattacg ttacaatacg tgttttcatc aaaatttccc taccgagcgg tatcctgttt
     2221 ttgaagacgg taggatttgg gtttgtttgg gggcaatccc gcccaaattg gagcaataga
     2281 tagggctgtg tccgaatatg cggctctgtg tgttgaaaca cagttaaaaa tacggaagac
     2341 tttatgtccg aacaacatat ttcgacttgg aaaagtaaaa tcaacgcatt gggtccgggg
     2401 atcatgatgg cttcggcggc ggtcggcggt tcgcacctga ttgcctcgac gcaggcgggc
     2461 gcgctttacg gctggcagat cgcgctcatc atcatcctga ccaacctctt caaatacccg
     2521 tttttccgct tcagcgcgca ttacacgctg gacacgggca agagcctgat tgaaggttat
     2581 gccgagaaaa gccgtgttta tttgtgggtt ttcctgattt tgtgcatcat ttccgccacg
     2641 attaacgctg gcgcggtcgc catcgtaacc gccgccatcg tcaaaatggc gattccctcg
     2701 ctgatgtttg atgccggcac ggttgccgcc ttgattatgg catcctgcct gattattttg
     2761 gtgagcggac gttaccgcgc tttggatcgc gtttccaaaa tcatcatcgt tactttgagt
     2821 atcgccacgc ttgccgccgc cggcatcgct atgtcgcgcg gtatgcagat gcagccggac
     2881 tttatcgagc cgacaccgtg gacgcttgcc ggtttgggct tcctgatcgc gctgatgggc
     2941 tggatgcccg cgccgattga aatttccgcc atcaattctt tgtgggtaac cgaaaaacaa
     3001 cgcatcaatc cttccgaata ccgcgacggg atttttgatt tcaacgtcgg ttatatcgcc
     3061 agtgcggttt tggctttggt tttccttgca ctgggcgcgt ttgtgcaata cggcaacggc
     3121 gaagcagtgc agatggcggg cggcaaatat atcgggcaat tgatcaatat gtacgccgtt
     3181 accatcggcg gctggtcgcg cccgctggtg gcgtttatcg cttttgcctg catgtacggc
     3241 acgacgatta ccgttgtgga cggctatgcc cgtgccattg ccgaacccgt gcgcctgctg
     3301 cgcggaaaag acaaaacggg caacgccgaa ttctttgcct ggaatatttg ggtggcgggc
     3361 agcggtttgg cggtgatttt ttggtttgac ggcgtaatgg cgaatctgct caaatttgcg
     3421 atgattgccg cttttgtgtc cgcccctgtg tttgcctggc tgaattaccg tttggtcaaa
     3481 ggtgatgaaa aacacaaact cacatcaggt atgaatgccc ttgcattggc aggcttgatt
     3541 tatctgaccg gctttaccgt tttgttccta ttgaatttgg cgggaatgtt caaatgataa
     3601 cgatgccgtc tgaaaccgca aaccgctttc agacggcatt gccgcgttta tggaaaaaaa
     3661 caccgacatc gcgtaaaata tgcgcaaatt ttgtgattcg gtcaggtcgt ctgaaacaga
     3721 ttgcgcctga tttatttttt cggaaaaccc tatgagcgaa caaaaccatc cgcaaaccga
     3781 gccgcagttg gacgaaaacc aaatcatcgc cctgcgccgc gaaaaactgc acaacatccg
     3841 ccaacagcgc aacgcctacc ctaacgattt caaacgcgac agctttgccg ccgacctgca
     3901 tacccaatac ggtgaaatcg gcaaagaaga actcgacccg caaggcattc ccgtcaaaat
     3961 tgccggccgc atgatgctca aacggcaaat gggcaaggcg agctttgcca ccattcaaga
     4021 cgtgtccggg caaatccagc tttatctgaa caacaaaggc gtgagccaag aaattttgga
     4081 cgacttcaac cattgggact tgggcgacat cgtcggtgcg gaaggcactt tgttcaaaac
     4141 caaccacggc gaactgaccg tacgcgtgtc cggcatccgc ctgctgtcca aatccctgcg
     4201 tccgctgccc gacaaacaca aaggtttgag cgatcaggaa accaaatacc gccaacgcta
     4261 tgttgatttg attgccaacg gagaatcgcg caataccttc atcaaacgca gccaaatcat
     4321 ccaatccgtg cgtaatttta tggtgggcga gcattatctc gaagtcgaaa ccccgatgat
     4381 gcacccgatt cccggcggcg cgacggcaaa acccttcgtt acccaccaca atgccttgga
     4441 cattccgctc tacctgcgca tcgcccccga gctgtatctg aaacgcttgg ttgtcggcgg
     4501 tttggagcgc gtgtttgaaa tcaatcgcag cttccgcaac gaaggtatgt ccgtgcgcca
     4561 caatcccgaa ttcaccatga tcgaattcta cgaagccttc tccgactacg aacgtatgat
     4621 gcagatggca gaagacatca tccgcaacgc atcgcgcaca gtaaacggta cggcaaacat
     4681 cacttacaat ggcaaagaag tcgatttgga aagcccgttc gagcgtctga ccatcctcga
     4741 agccatcaaa aaatacaatc cgcactacac cgacgagcag ttgaacgatg cggaatggct
     4801 gaaaaaagaa atcgtcaaac acggtgaaag cctgccgccg tccccgggca tcggcagcct
     4861 gcaactcgcg ctgtttgaag gttgcgccga gggcaagctg tggaacccga ccttcatcat
     4921 cgattacccg gtcgaagttt ccccgttggc gcgcgcttcg gataccaaac aaggtctgac
     4981 cgaacgtttc gaattgttcg ttgtcggccg cgaactggca aacggctatt cagaattgaa
     5041 tgaccctgaa gaccaagccg aacgcttcaa atcgcaagtc gcacaaaaag acgcgggcga
     5101 cgacgaagcc atgcattacg atgccgacta catccgcgcg atggaatttg gtttgccgcc
     5161 gaccggcggt tgcggcatcg gtatcgaccg cttggtgatg ttgctgaccg attcgcaaac
     5221 catccgcgat gtgattctgt ttccgcagat gcgccccgag tagtcataaa aacagttgaa
     5281 atggcaatgc cgtctgaacc cgattggatt cagacggcat tttgtatggc ggtacggatt
     5341 tattcgcttt ctaactgacc gacccattcg gacaaggcag tcaggcgttg ttcggatttc
     5401 gccacgaacg ggttttcggt atcccacgcg tagcctgcca aaatcgaaga aagcatacgg
     5461 ctgatttccg ggctgcggtc gggcgcgtac acgacgtttt ggaagcggaa atcgtaccaa
     5521 ccgtccacac atcaggggtg gtaatccgaa tcaacacgcc ttctcgcctg tgcggcggac
     5581 ataaaccgtt ttttgacctc cagcaccaca cctaaaatcg tgctgccttc gggcaggtca
     5641 aaggcttcag acggcctgcc cgtctgataa atcagcatcc tgcccgcaat atcgataaca
     5701 aggtgcgcga cgaccagccg cttgtcggac agttcaatca gcacgatgtc ctcgtccgca
     5761 gggcgcatac gcggctcgac caccaccaaa tccccttttt cgataaccgg cgacatggac
     5821 gtgtcgggca tggcaacggc aaacgtgtac tgcgaggggg cgaccaccga agcaaacaag
     5881 tcgaaactgc ctgtgagttt gcctgtacgg gcgaaagccg ccgcctgacg gtctgacaac
     5941 agggggcgtg gtgtgagcct catcgacagt gctttgccgt cggcatcgct gtggttgtgc
     6001 gtatgcgtat gatggttgtt gatggtcgta tcgccttgga catcgttaag gactatgctg
     6061 tttgaacctg ccacggattt ggtcatctca ttgtaggctt gcgtcgtgat gcgctcttgc
     6121 ttggtcatac ctgttacagc ctgtttcagc atttctaacg ctatgtgatt cttcctaagg
     6181 gtcgctatac cggcttttgc agcttgctca ttaccacaag aggggcaacc ccagctactt
     6241 cctatgaaca tattggagta cctcgaacag gtttggtttc catgtttagg gcagttgatt
     6301 acacacggct tggctgtacc ctcaaactcg accaacttaa tgccacgatg accgaaacgt
     6361 tcctcaacct cagcagcagc ttcttcaaat gtttttgacc ttcttgccat attgacctcc
     6421 ggattattga ttattttggc tatgctttaa cgagggattg gaatgaggta aagctgttgc
     6481 cctctccccg caatagttcc attaggcgcg gatgaatgaa tagtttgtcc ctgccgatgg
     6541 cgatttcttg cagcacacct atgtctgaaa gctctttcag gtacttagag gccgtctgcc
     6601 gtttggctat ccctgccgct tctaggttgg caatgcgtgt atatggctgc tcaaacagaa
     6661 gatttaccag ttcgtgcgtg tagattcctt gtgcgtgtgt ccgtatgtgt tgccgtgtct
     6721 gctcgaacag gcggcgtatc gcatctattt tcgataccgt ccaatcggcg gtgtcagcta
     6781 cgccgtctaa gatgtagatt atccagcttt cccagtcctg ccgttcggtt acgcctaaaa
     6841 gcaggcggta atagtccgcc ctgttttcga tgatgtagcg gctcaaatac aaaataggca
     6901 aatccaaaag ccctttttca atcaatagca ggctgttcaa tatgcgcccc gtccgcccgt
     6961 tgccgtccgt aaacggatgg atggcttcaa attggtaatg tgccgccgcc atgatgataa
     7021 gcgggtctaa atcgccgctt tcgtgaataa accgctccca atttgccagc ttgccgcgta
     7081 tggtttcttc tccttcgggc ggggtataga caacatttcc gctgttgcct ccttttaggg
     7141 ctgtgccgcc tgttttgcgg atggccattt cgtaggggtg cttgatggcg ttgcagacca
     7201 tgatggcggt ttgtgtgcat aaagggcggc tcgtcagtga ttcatagcct gcaaacaggg
     7261 cggtgcggta ttgcagggct tctttcgtgg cagggtcttg ccgttccgta tccatttgca
     7321 gggattgaaa cagcttgtcc gtggtggtta cgatgttttc aatttccgaa cttgcacggg
     7381 cttccataac aggaagggtg ttaatcagca tggcttgatt cggtatcaat tctgccgcct
     7441 gctttaaacg ggcaagggat gcacgggcgg ctatacaacg tttcaggatg gttttgcttt
     7501 caatatcctg ttttggcggc aggggtggta aatcgttata gggaatatcg ggtttccagt
     7561 tgctcatgtt taaaatttcg gaaaatttaa agatgtttcc agtatatatt tacgccgtgt
     7621 atatatcaag gatatatgtt taaaaatttg gcttttgtaa atatatgggc ggtaaaaccg
     7681 cattatttta tgttgttacc tattttacac cttgtctgca cacgatttcc aaacaaccta
     7741 aaaagaaacc tgccgaaaga caggtttaaa gaggtcattt taaatgtgaa ctaaagttta
     7801 caccctcgga gggtatcgcg tatatcagac gtacttaata tacactat
//
LOCUS       DAWHXZ010000087         7846 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000087 DAWHXZ010000000
VERSION     DAWHXZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.087"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(94..774)
                     /gene="tsaA"
                     /locus_tag="WIE35_001180"
     CDS             complement(94..774)
                     /gene="tsaA"
                     /locus_tag="WIE35_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ4154096.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAEVCIELNPKFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            888..1523
                     /locus_tag="WIE35_001181"
     CDS             888..1523
                     /locus_tag="WIE35_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ4154097.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSTKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            1560..2528
                     /gene="waaC"
                     /locus_tag="WIE35_001182"
     CDS             1560..2528
                     /gene="waaC"
                     /locus_tag="WIE35_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ4154098.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVETYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTAPEAGRLKNLEQPYYAALHATSRDSKLWPVENWRALLQKLNEEQQCNIYLPWGS
                     EVEKTRAEQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQTLMDCVAADKG"
     gene            2754..3503
                     /locus_tag="WIE35_001183"
     CDS             2754..3503
                     /locus_tag="WIE35_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ4154099.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIEGGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKTLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            3514..4449
                     /locus_tag="WIE35_001184"
     CDS             3514..4449
                     /locus_tag="WIE35_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ4154100.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKIDLLVAGSGASAVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYCYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            4512..5123
                     /locus_tag="WIE35_001185"
     CDS             4512..5123
                     /locus_tag="WIE35_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ4154101.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGMQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(5175..5492)
                     /locus_tag="WIE35_001186"
     CDS             complement(5175..5492)
                     /locus_tag="WIE35_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ4154102.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            5650..6921
                     /gene="purD"
                     /locus_tag="WIE35_001187"
     CDS             5650..6921
                     /gene="purD"
                     /locus_tag="WIE35_001187"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ4154103.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVASGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARIVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            6955..7557
                     /locus_tag="WIE35_001188"
     CDS             6955..7557
                     /locus_tag="WIE35_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ4154104.1"
                     /translation="MTNVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(7628..>7846)
                     /locus_tag="WIE35_001189"
     CDS             complement(7628..>7846)
                     /locus_tag="WIE35_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ4154105.1"
                     /translation="IYYRINHSKEFADRQNHINGIENFWNQAKRVLRKYNGIDRKSFP
                     LFLKECEFRFNFGTPSRQLKILQDWCGI"
BASE COUNT         2079 a         1544 c         2102 g         2121 t
ORIGIN      
        1 ttaatccgct ataacttgat attgcggctt atggcgaaca aacaacagcc gtttgttcaa
       61 ctgctatgcg gcaaaaccgg atttttgccc gatttaaagc ggggttgggg aaagatcaat
      121 aacggttgca cggttttcct cgatttgaaa tctgacttcg taatctgcaa tattcatcac
      181 ataaatccgt tcgggaatat tctgataggc ggggcgcgga tcttgggcaa tgctttggct
      241 gataaggttt ttggtgtttg cagataaatt ttccgcgccg atgttttcct gccaaacgac
      301 ttccaactct acgggtttgc cgctgacgaa accggatgcg gcatcgggtt tggattcgac
      361 aaaggggata taaggtttga tgtccacaat cggtgtgccg tccagcaggt ctgcgccgct
      421 gcaatagagg cggacgggtt tgccggtttc gatgcgttcg agtttcagga gcgagagtcc
      481 gagatggttg gggcggtggg ggctgcgcgt ggcgaacacg cccatttttt gtttgccgcc
      541 gagccgtggc gggcgcacca tttgcgccca gccttcatcc aatacgccgt gaaaaataaa
      601 acttatccac acataatcga aatcttccag cccgcgcacg ctgtctgcgg tgaatttggg
      661 attcagctcg atgcatactt ctgcggcaga gaccaaaccg ggctggcggg cgatgccgaa
      721 tttctgtttg tagggcgagc gggcggtgcc gatgggggta atggtataag tcataaggtt
      781 gtggataagt tttttggaaa aaccttatct tatcacaatc ggttttaccg cttttgggca
      841 gtaatttgtt ataatacagg gcattttaat cgcgttgaaa taatgagatg attgtttcca
      901 ttgatgttga tgcacaaaaa acgtttacgc cactgtgtcc tgacgaactg cccgtgaacg
      961 aggggcattt gattgtcgag gagttgaatg cgcaagccgc tttggcggat ttgcgcgtga
     1021 tgacgaaaga tgcgcatcat atggtagcga aatggcttgt ggataaccct gttgatatgt
     1081 tgaagccgac aggtttgcct gatgcggatt tgacttgggt ggctcatgcg atggtcggta
     1141 cgcgcggcta tgaattattg gacggactgc cttctaccaa agaatacgat tattgcgttt
     1201 ggaaaggtgt tgatcctgaa ttgcatcctt acggcgcgtg ttttcacgat attgaggaaa
     1261 aactaagcac agggctgatt gaatggctgc gttgtcaaaa tacggatacg gttattatcg
     1321 gtgggttagc tacggattat tgtgttaaaa caacggtttt gcagttactc aaaggcggtc
     1381 gttggcaggt tgtcgtcaat gaagcggctt gtcggggtat tgcgccggaa accatcgaaa
     1441 cagcatggca ggaaatgcat tctgctgggg caatcatctt aaaaaacgct gatgaaataa
     1501 ataaatatat taataatcaa taagttaaaa ttgtttcacg tgaaaccccc ttcctaaata
     1561 tgaaaatttt gcttgtccgc ttgtctagta tgggcgattt aattcacact ttgcccgcaa
     1621 tcgaagattt ggcgcgacaa tgtcctgatg tagaactgca ttggctatgt gaggctggat
     1681 ttgcagatat tgcgcgcctg catccgtttg taaaaaaaat ccatgtgatg aaatggcggc
     1741 aatggcgcaa acatctcttt cgggctgaaa cttggcggga aatgggtcat ctgaaacaga
     1801 ctttgcggca ggaagtattt gatttcgtat tggacagtca aggtctgatt aaaagcgcgt
     1861 gtttcgccaa aatggcaaaa tccccgattt gtggtttgga taaaaacagt gcgcgcgagg
     1921 gatgggctgc tttggcgtat gtagaaacat acgctgtacc gaagggaaaa aatgccgttt
     1981 ggcgcaaccg tgaactgttt gcccaagtat ttgggtatgt aatgcctgaa acgcaggtat
     2041 ttggcttgac tgctcctgag gcaggtcgtc tgaaaaattt agagcagccg tattatgcgg
     2101 ctttgcacgc gactagccga gacagtaaat tatggcctgt ggaaaactgg cgggcgttac
     2161 tgcaaaagtt gaatgaagaa cagcaatgca atatttacct gccttgggga agtgaagttg
     2221 aaaaaacgcg tgccgaacag attgcagacg gactgccgtt tgctattgtg tgcgacaaaa
     2281 taaatttatt gcaggcagcg tatctgctga aatacgcggt cggaattgtc ggcgtggata
     2341 ccggtttgct gcatttggca aatgccttgg aaaaacctgt ggtcggcatt tataccgata
     2401 ccgatccgat taaaacaggc gttcaggttt cgccaattgc aaaaaatttg ggcaatatcg
     2461 ggcagattcc gaccgcagat ttggtttatc aaacgttgat ggattgtgtt gcagcagaca
     2521 aaggctaaag ggttgtctga aactgatata gcacctgata tttttcagtt tgttaagcat
     2581 aagaatgtat atctaccagc ctgagtaaat gctttaaaaa tggtataatc ggcaagcatt
     2641 atccgaaaga tttttgaatg aattcgtttt taaggaaaac ggatttggtt ttatagggaa
     2701 gaaggtttgg tttcgctgga tggtgaaagc agcgttcttt ttaggagggt tcaatgaaag
     2761 cactggtcgc agtaaagcgc gtagtggact acaacgtcaa agttcgtgta aaagccgatg
     2821 gttcggatgt ggatatcggc aatgtcaaaa tgtcgatgaa tccgtttgac gaaatcgctg
     2881 tggaagaagc tgtccgtttg aaagaagccg gaaaagtaag cgaaatcgta gcggtttctt
     2941 tgggtgagaa aaaatgcgaa gaaaccttgc gtacagcttt ggcgatgggt gccgaccgtg
     3001 ccattcatgt tgaaaccgat actaaactgg agtctctggc cgttgctaag ttgctgaaag
     3061 ccgttgcgga caaagaaaat ccgcaaattt tctttttggg caaacaagcg attgatgatg
     3121 atgccaatca agtggcgcaa atgctggcag ctttgctgaa tgcggcgcaa ggtacgtttg
     3181 cttcaaaagt acacattgaa ggtggtgaag tacagattgt gcgcgaaatt gatggcggcg
     3241 aagaaaccat agcattaaaa ctgcctgctg ttatcagtgc tgatttgcgt ttgaacgagc
     3301 cgcgctttgt caaactcccc aatattatgg cggcaaagaa aaaaactttg gaaaaactga
     3361 ctcctgacga tttggttgcc gacatttcac ctcgtttgaa aacggtgaaa tttgccgaac
     3421 ctaaggcgcg tcaggcaggc gtaaaagtag caagcgttgc cgaattggtt gaaaaattga
     3481 aaaacgaagc caaagtgatt tgaggaggct gaaatgagcg tattgattat tgtcgaacac
     3541 gacaacaaac agttgaatcc taccactttg catgctgtta ccgctgccgc taagctgggt
     3601 aaaatcgatt tattggttgc cggaagcggt gcatctgctg tagtagagtt cgcaaaacaa
     3661 gtggcgggtg tggaaaaagt tttggttgca gatgctgccc attatgccga aggtttggct
     3721 gaagagttgg ctccgctggt tgtcaaattg gcagcggatt actgctatgt tgcagcaacg
     3781 gcaactacat tcggtaaaaa ccttttgcct cgcgtagcag ccttattaga cgtaccgcaa
     3841 atttctgatt tgaccgaaat tgtggataac acgacttttg tgcgccccat ttatgcgggt
     3901 aatgcatttg aaaccgtgca agctgattca gaaaaattgg tgctgacctt ccgtgcgacg
     3961 gtttttgacg cagtggcggc gcaaggtggt aatgctgagg taattaatgt tgaagcaacc
     4021 cctgcccaaa acctgagccg ttttgttaac cgtcagcttt cccattccga tcgtcctgaa
     4081 ctgactcagg caaaagtgat tgtttccggt ggtcgtgcgt tgggtagtgc ggaaaaattc
     4141 aatgaagtgc tgacaccgtt ggcggatgtt ttaggtgcgg caatcggtgc atcccgtgcg
     4201 gcggttgatg ccgaatatgc gccaaacgat gctcaagtcg gacaaaccgg taaagtggtt
     4261 gcgccgcaac tctattttgc aatcggtatt tcaggtgcga ttcaacacgt tgccggtatg
     4321 caagacagta aggtgattgt tgcaatcaat aaagatgctg atgcgccgat tttcaatgta
     4381 gccgattacg gattggttgg cgatttattt gaaatcgttc cacaattaac agaagcattg
     4441 aaaaattaat gtttcacgtg aaacgttcag acgaccttgt tattaatgag gtcgtctgaa
     4501 aacatttcag catgattacg acttataaaa ccattacttc ctcgacgcag gctgagttta
     4561 aagataaagg cagccgtttt attgcatttg cctatccgat tcggacattg gctgatgtga
     4621 aaaaatacct cgatccgtta aaggaagagc atcataaagc acgacactgg tgctatgcct
     4681 atcgtttggg tgtggatggc atgcaatttc gtgccaacga tgatggagag ccatcaggaa
     4741 gtgctggacg accaattttg ggacagattg attcggtggg tattaccgac gttttggtcg
     4801 tggtcgtccg ctatttcggt ggtactttgt taggtgttcc aggtctgata catgcgtaca
     4861 aagaggcgac ggctcaagcg ttggcagttg cagaagtagt tgaaaagaat attgaaaaaa
     4921 ctgtttggct gaaatgtgaa tatccgtttt tgaatgaagc gatacgcatt gctaaacaat
     4981 atcaggcgga tgtattggag cgggatttgc agttggattg ccgattgacg gtcagtttgt
     5041 cgttggtaaa ttatgaggcc tgtgttgcgg cttggaagag tacacggcag attgaagtaa
     5101 atacggaaaa gccttttgaa tgaaaggtcg tctgaaatgc tttgtgatgg tatttcagac
     5161 gacctcttcg ctgactaatc ttcttgtgta tatttgtcta aggcatcgac gctggagcgc
     5221 aaatataacc aagccagttg atggaattcg cccagcgcgt tccatagtgc atcttcactc
     5281 ataatgctgt attcgccttc tgtgcgcaaa tccacctccg ctccatcttc aatagcccga
     5341 tgacaagcct gatattcgtg ggtataaaaa ttgtccatat cgcgaatcaa agcgacggca
     5401 tgtttccaac ggcggatgtc ttcttccaat tgcggtagta aatgacacca ttcgcggtat
     5461 ttgctttgga tttcatcaat acgtttttgc atggttgctt gtcctttttt aatttatgaa
     5521 taagttgaat aggttgcaga gtggaatgaa gcggtctttt gtttgattct actaaaattt
     5581 cttcagatgg gataaaagtc cctttgtatc gagttttcgt taaaagtaca tatttgtcaa
     5641 ggagatggca tgaaactgct ggttatcggt aatggcggtc gcgaacacgc gctggcttgg
     5701 aaattggcgc agtcgcctaa agtggaaaca gtatttgttg cgtctggtaa tgccggtaca
     5761 gcgattgaac ccaagctgca aaacatcgac ttgactgcgc atcaggattt gattgaattc
     5821 tgtcgtaaag aaaatattgt ttttaccgtc gtcggccctg aagcgccttt ggcggcaggt
     5881 gttgtggatg atttccgtgc tgcaggacta aaaatattcg gtccgacaca atatgcggcg
     5941 cagttggaaa gttccaaaga tttcgccaaa gcatttatgg cgaaatacaa tattccgacc
     6001 gcgcaatatc aaacctttga aaacgccgat gccgcacatg attacgtcaa tcagaaaggt
     6061 gcgcctatcg ttatcaaagc cgatggtttg gcggcaggta aaggcgtgat tgtggcgatg
     6121 actttggatg aagcgcatgc tgcgattgat gatatgctgc tggacaacaa aatgggcaat
     6181 gccggcgcgc gtattgtgat tgaagatttc ctgcaaggcg aagaagcgag ttttatcgtc
     6241 atggttgatg gcaataatgt actgcctatg gcaaccagcc aagaccacaa gcgtctttta
     6301 gacggcgaca aaggtctgaa tacgggcggt atgggtgcgt acagccctgc gcctgtggta
     6361 acgcctgttg tgtacgaacg cgcgatgaat gaaattattt tgccgaccgt agcagggatg
     6421 aaagcggagg ggcatgagtt caccggtttc ctgtacgcag gtttgatgat tgatcaaagc
     6481 ggtgcacccc atacgattga gtttaactgc cgtttcggcg atcctgaaac ccagccgatt
     6541 atgagccgac tgaacagcga cttatcggat ttggttgaag cggcaataga cggcaaactc
     6601 gatagcgtaa ccgcggaatg gagcccgcaa actgccgtgg gcgtggtact ggcggcgcaa
     6661 aattaccccg aaacgcccaa aaaaggcgat gttatttccg gtctggatgc tgccaaccaa
     6721 gtaggcaaag ttttccatgc aggtacaacg gcaaacgaga aaggtgacgt attgaccaac
     6781 ggcggacgcg tattgtgtgt tgtgggattg ggcgataatg tggcgcaagc gaaagctaag
     6841 gcatatggcg cgttggaaaa aatcagtttc gacgggatgc aataccgtaa agacattgcc
     6901 gataaagcga ttaaccgtta attagaaaga ggatgatgcc gtctgaagcc aagtatgact
     6961 aatgtgcttc agacggtatt ttatattatg ttatttccga gaattatcgc agcatcagcg
     7021 caacgtaagt taagcgttta tttgaaaaaa ggtggtttgg tcgcttatcc gaccgaatct
     7081 tgttatggct tggggtgtct tccgacatta gccaaagcac tcggtaagtt ggcgcatttg
     7141 aaaaaaagac cgcaacacaa gggtatgatt gtcatcggga atcaatttga acaattgcag
     7201 cctttacttc aaatgccgtc tgaaaatctt caggatatgc ttagaaaaga atggcccgcc
     7261 cctaaaacgt tcctattatc cgccaaatcc tgtgtcttac ccgagttacg cgggaaacag
     7321 cgcagcaagt tggcggtcag ggttcccgcc catgtaggtg cgcgccgctt gtgtcaggct
     7381 ttgcaaacgc ctttggtctc gacttcgtgc aaccgcgcag gcaaacgcgc gtgtcggacg
     7441 gagagggaag tcagaaggca attcggacgc gatgtgtgga ttgttggtgg gagaatcggc
     7501 aggcagaaat cgccgagtca gattattgac ggggaaacag gtaaacgttt gcgttagata
     7561 tgggtacttg aatgaaaact tttttgcata cacaaagaaa aaattttggg gctgtactag
     7621 attagcccta aatcccacac caatcctgta ggatttttag ctgccgggac ggtgtgccga
     7681 agttaaatcg aaattcgcat tctttcaaga acagcgggaa agatttgcga tcgattccat
     7741 tgtattttcg caagacacgt tttgcctgat tccaaaaatt ctcaatgccg ttaatgtggt
     7801 tctgacggtc tgcaaattcc ttggaatggt tgatgcggta ataaat
//
LOCUS       DAWHXZ010000088         7650 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000088 DAWHXZ010000000
VERSION     DAWHXZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.088"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(9..425)
                     /gene="dksA"
                     /locus_tag="WIE35_001190"
     CDS             complement(9..425)
                     /gene="dksA"
                     /locus_tag="WIE35_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ4154106.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(572..1363)
                     /locus_tag="WIE35_001191"
     CDS             complement(572..1363)
                     /locus_tag="WIE35_001191"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ4154107.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRERLQKEL
                     GVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTLSRYIGG
                     TRRIVRVMPNTPGKIGLGVSGMFAEAEVSETDRRIADRIMKSVGLTVWLDDEEKMHGI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1454..1819)
                     /locus_tag="WIE35_001192"
     CDS             complement(1454..1819)
                     /locus_tag="WIE35_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ4154108.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIRKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKK"
     gene            complement(1819..2505)
                     /locus_tag="WIE35_001193"
     CDS             complement(1819..2505)
                     /locus_tag="WIE35_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ4154109.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGRDPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTIC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVVLAVEVAKLPNIVVRG
                     LMCVAKAGGSEAELKTQFQTMQKLLADLNTAGVKADVLSMGMSDDMPAAIACGATHVR
                     IGSAIFGKRG"
     gene            2625..3668
                     /locus_tag="WIE35_001194"
     CDS             2625..3668
                     /locus_tag="WIE35_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ4154110.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3831..5057
                     /locus_tag="WIE35_001195"
     CDS             3831..5057
                     /locus_tag="WIE35_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ4154111.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5343..7493
                     /gene="yccS"
                     /locus_tag="WIE35_001196"
     CDS             5343..7493
                     /gene="yccS"
                     /locus_tag="WIE35_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ4154112.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQENVANAYEALGSYLEAKADFFDPDEAEWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKIAERLKSGETADDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1891 a         2541 c         1666 g         1552 t
ORIGIN      
        1 tgccgccatc agtcggcaaa ctgttttttc atcctctctc ggcgttcttg ggactcaaca
       61 gataaagtgg ctgtcgggcg tgccagcagc cgcttcagac cgataggctc tcccgtatcg
      121 gcacagaatc cataatcccc ttcatcaata ttgcggatgg tcgcctgtat tttactgaga
      181 agtttccgtt cccgatcgcg ggtacggagt tccaatgcgt actcttcttc ctgtgtggca
      241 cggtcggcag gatcgggggc tgattcgtgt tcttggagat gccctgtcgt agcggaagca
      301 ttttcgatga gttcgtcttg catttttacc agcaattcgc ggaaaaaagc caaatggtcg
      361 tcattcatat aatcgtcttc cggcccgctc caattcaaaa tatcttgttc tgtcagcttt
      421 gccataatgt atatcctctc ggttcaccgc aaaaaacaaa gcaccacaaa gtggctttat
      481 tcctttgatt tttataaggc ttattgccta aggcgttttt gaaaacgcgc atcataacac
      541 gttttggatt ggttttttta ttttctttac attattgata ttgccgttcc atttcctgcg
      601 aacggcgcac acaggcacaa acgccctcgc ttatggcttc ggcgacacga tgccgcctga
      661 aagcttccac ggcttcgtgg gttgtcccgc cttttgacgt tacatttttt tgaagcttct
      721 cgaaatcttc acccgtctgc tcggcaaggg caaccgctcc tttaaacgtt gccagactga
      781 gcgcgcgtgc ttctgccata tcaaaccctt gtcggatggc ggcattttgc aatgcgtcca
      841 gcagataaaa cacataagcc ggtccgctgc cgctgatacc ggtaatgccg tgcatttttt
      901 cctcatcatc caaccaaaca gtcaaaccga ctgatttcat gattcgatcg gcaatcctgc
      961 ggtctgtttc cgatacttcc gcttcggcaa acataccgga tacgcccagc ccgatttttc
     1021 cgggtgtatt cggcataacc cggacaatgc ggcgtgttcc cccaatgtaa cggctgagcg
     1081 tatcgaccgg caatccggct gcgacagaaa gcaccaatgc gccgttggtg cggatatttt
     1141 tgcacgcggc ttccatatcc tgcggcttga cggcaaggat taaaacatcg tcggaatgaa
     1201 gctccggcaa ggtttcagaa gtttcgaccc ccaactcttt ttgcaaacgt tcgcgctttt
     1261 ccgcaccccg attggctata tggatgcggt aaccgccttg tttgaccaat ccgcccgcaa
     1321 cggcagccgc catattgccg ccgccgagaa aataaacatt cattatttct accctattca
     1381 aaattacctg actaaattca ttcccaattt tttaccatcg gacgcgctcg gacagaccgt
     1441 tgcgccgcaa cccctacttt ttcaaacaat caaaaccttc ctgtttgaac gtctcaatca
     1501 aaggtttgcc gtcacgcgta cgccaatcca acccgcaggc ttcaaacttc cttattttcc
     1561 tgtaaaagta agattgttgt gcggattcat attcatttgc cctgacggaa cgattgccgt
     1621 ctgaaagcgg tatctcgtag tcctctaaag acggcggact ctcgccgcca atatcccaaa
     1681 aatcgatggc ggcaggtttt ctcggcttac accagccgga cagggacgaa cactcatacc
     1741 aacctgccaa ccggcagcct gcaaccgtaa atgcaagcaa cgcagccatt gccgtatatt
     1801 ttattgcccg aatttccatc agcccctttt cccgaaaatc gcactgccga tacggacatg
     1861 tgttgcaccg cacgcaatgg cggcaggcat atcgtccgac atccccatag acagcacatc
     1921 cgccttaacg ccagccgtat tgaggtcggc aagcaacttt tgcatggtct gaaactgggt
     1981 tttcaattct gcctcgctgc cgccggcttt ggcaacacac atcagtccgc gtacgacgat
     2041 attcggcaac ttcgccactt cgaccgcaag cacgaccgct tcttcgggcg cgacaccgtg
     2101 tttcgccgcc tcgcccgcaa tgttcacctc gatacacacc tgcaaaggcg gcattgagga
     2161 aggacgttgc ccgctcagcc ggacagcggt tttcagacgg catatggtat gcacccaatg
     2221 cgcgcgttcg gcgacaaact tggttttgtt ggactgcaca tcgccgatga cgtgccacac
     2281 gatgtcggtc aaatccgcca actcttccgt tttgccgtac cactcctgaa tatagttctc
     2341 gccgaaatca cgctgtccgg cggcgtaaac ttcgcggatg ccgtctgaag ggaaagtctt
     2401 accgacggca atcaggctga cggaatgcgg atccctgccc gcctgcagaa ccaattttcc
     2461 gatacggtcg gacacctcac aataacgttc ttgcaacacc gtcatagatt atcccctaat
     2521 taaaaatgat taaacagttg aaacccctcc agtcagggcg gtacaatcaa ggttgttaga
     2581 accattccaa ccaatcgaaa cattatacta aacagagccg cattatgcag attaccgact
     2641 tactcgcctt cggcgctaaa aacaaagcat ccgaccttca cctgagttcg ggcatatccc
     2701 ctatgattcg ggttcacggc gatatgcggc gcatcaacct tcccgaaatg agcgcggaag
     2761 aggtcggtaa tatggtaact tcggtgatga acgaccacca gcggaaaatc taccagcaaa
     2821 acttggaagt tgacttctcg ttcgaactgc ccaacgtcgc ccgattccgc gtcaacgcct
     2881 tcaacaccgg ccgcggtccc gccgccgtat tccgcaccat tcccagcacc gtcttatcgc
     2941 tggaagaatt gaaagccccg agcattttcc aaaaaatcgc agaatcgccg cgcggcatgg
     3001 tattggttac cggccctacc ggttcgggca aatcgaccac gcttgccgcg atgatcaact
     3061 acatcaacga aacccagccg gcacacatcc tgaccatcga agacccgatc gaattcgtcc
     3121 accaaagcaa aaaatccctg attaaccagc gcgagctgca ccagcacacc ctcagcttcg
     3181 ccaacgcgct gcgttccgca ttgcgcgaag accccgacgt tatccttgtc ggcgagatgc
     3241 gcgaccccga aaccatcggc ttggcactga ccgccgccga aaccggacac ttggttttcg
     3301 gcacgctgca cacgaccggc gcagcaaaaa ccgtcgaccg tatcgtggac gtattcccgg
     3361 cgggagaaaa agaaatggtg cgctctatgc tgtccgaatc gctgaccgcc gtcatctccc
     3421 aaaacctgct gaaaacgcac gacggcaacg gccgtgtcgc ctcgcacgaa atcctgattg
     3481 ccaaccccgc cgtccgcaac ctcatccgcg aaaacaaaat cacgcagatt aactccgtcc
     3541 tgcaaaccgg gcaggcgagc ggtatgcaga caatggacca atcgctgcaa tcgctggtgc
     3601 gccaagggct gatcgcaccg gaagtcgcac gcagacgcgc gcaaaacagc gaaagtatga
     3661 gtttctgaca cacaaccgcc ttccggccat accggcggga aaacaaggcg caaacacgcg
     3721 gggcgggacg cagcatcccg cccggctacc tttccgaaca agaagcgtcc gccttcctgt
     3781 tgaaacctgc cgccgcaaac tgcaaggctt aaaccgaaaa gaagctaacg atgaataccg
     3841 ataacctgca cgacatcttg gacgaaatgg ttcaagtgta ttctcaaaaa aaacaaagcc
     3901 gatccgaaac cccggccgaa atcggcgcac acttccaccc gctgctcgac cgcctgtgcg
     3961 aaaccgcaga agcacaaaac gcgtccgaca tccttatcag caaaggattc ccgccctcgt
     4021 tgaaaatcaa cagcgcatta accccgcagc cgcaaaaggc gctgacgggc gaggaaaccg
     4081 ccgccatcgc cgcatcgacg atgaacgccg aacaatcgga aatattccgg cgcgacggcg
     4141 aaatcaacta ctccgtccag tcgcgcagcg gcacgcgcta ccgcgccaac gcctaccaca
     4201 gccaaggcag cgcaggtttg gttttgcggc gcatcaacca cgtcatcccg caaatgcagg
     4261 aattgggcct gcccgaaaaa ctcaaagacc tcgccgtcgc accgcgcggg ctgctgatta
     4321 tcgtcgggcc taccggttcg ggcaaatcca ccacgatggc gactatgctc gaacaccgca
     4381 acaaaaccct gcccagccat atcgttacca tcgaagaccc gattgaattt atctacaaac
     4441 cgcgccgctg catctttacc cagcgcgaaa tcggcgtcga caccataaac tggcagacgg
     4501 cggtacaaaa cgctatgcgc caatcccccg acgtggtctg catcggcgaa gtccgcagca
     4561 gggaaagtat ggaatacgcg atgcagctcg cccaaaccgg ccacctgtgc atttttacgc
     4621 tccacgccaa caccgcgccg cagtcgctcg aacgcatact caacttctac cccaaagaac
     4681 agcacaacca aatactgatc gacatcgccc tcaacctgac cggcatcatc tgccaacgcc
     4741 tcgccctcaa acaagacaaa acgggcagga cggcggttgt cgacttgctc atcaacacgc
     4801 ccgccatcca agacttcatc ctgaagggcg acctgatgaa catcagtaaa atcatggaaa
     4861 ccgccaaaac cgacggaatg cagacgatgg atcaaaacct tttcgaactg taccgtcacg
     4921 gcatcatcag ttacgaagaa gccctgcgcc agtccgtttc cgccaacaac ctgcgattgc
     4981 acatccaact gcacaaagaa ggcaaaacgc ccgaactcct ttacgacagg gtcaacggtc
     5041 tcaacctcat ttcctgatcc gcaaaaccca atgccgtctg aaaaccgcat ccccgttttc
     5101 agacggcatg attttatccg ccccattcat gtgctacact ttatagtgga ttaaatttat
     5161 agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagcacaa atagtacgga
     5221 accgattcac ttggtgcttc agcaccttag agaatcgttc tctttcagct aaggcgaggc
     5281 aacgccgtac tggtttttgt taatccacta tattcacttc ataacaataa accggtaaaa
     5341 ccatgaaaac cccactcctc aagcctctgc tcattacctc gcttcccgtt ttcgccagtg
     5401 tttttaccgc cgcctccatc gtctggcagc taggcgaacc caagctcgcc atgcccttcg
     5461 tactcggcat catcgccggc ggccttgtcg atttggacaa ccgcctgacc ggacggctga
     5521 aaaacatcat cgccaccgtc gccctgttta ccctctcctc gctcacggca caaagcaccc
     5581 tcggcacagg gctgcccttc atcctcgcca tgaccctgat gaccttcggc tttaccattt
     5641 taggcgcggt cgggctgaaa taccgcacct tcgccttcgg cgcactcgcc gtcgccacct
     5701 acaccacact tacctacacc cccgaaacct actggctgac caaccccttc atgattttat
     5761 gcggcaccgt actgtacagc accgccatca tccttttcca aatcatcctg ccccaccgcc
     5821 ccgttcaaga aaacgtcgcc aacgcctacg aagcgctcgg cagctacctc gaagctaaag
     5881 ccgacttttt cgatcccgac gaagccgaat ggataggcaa ccgccacatc gacctcgcca
     5941 tgagcaacac cggcgtcatc accgccttca accaatgccg ttccgccctg ttttaccgcc
     6001 ttcgcggcaa acaccgccac ccgcgcaccg ccaaaatgct gcgttactac tttgccgccc
     6061 aagacataca cgaacgcatc agctccgccc acgtcgatta tcaggaaatg tccgaaaaat
     6121 tcaaaaacac cgacatcatc ttccgcatcc accgcctgct cgaaatgcag ggacaagcct
     6181 gccgcaacac cgcccaagcc ctgcgcgcaa gcaaagacta cgtttacagc aaacgcctcg
     6241 gccgcgccat cgaaggctgc cgccaatcgc tgcgtctcct ttccgacggc aacgacagcc
     6301 ccgacatccg ccacctgcgc cgtcttctcg acaacctcgg cagcgtcgac caacaattcc
     6361 gccaactcca gcacaacggc ctgcaggcag aaaacgaccg catgggcgac acccgcatcg
     6421 ccgccctcga aaccggcagc ctcaaaaaca cctggcaggc aatccgtccg cagctaaacc
     6481 tcgaatcagg cgtattccgc catgccgtcc gcctgtccct cgtcgttgcc gccgcctgca
     6541 ccatcgtcga agccctcaac ctcaacctcg gctactggat actgctgacc gcccttttcg
     6601 tctgccagcc taactacacc gccaccaaaa gccgcgtccg ccagcgcatt gccggtaccg
     6661 tactcggcgt aatcgtcggc tcgctcgtcc cctacttcac cccgtctgtc gaaaccaaac
     6721 tctggattgt catcgccagt accaccctct ttttcatgac ccgcacctac aaatacagtt
     6781 tctccacctt cttcattacc attcaagccc tgaccagcct ctccctcgca ggtttggacg
     6841 tatacgccgc catgcccgta cgcatcatcg acaccattat cggcgcatcc cttgcctggg
     6901 cggcggtcag ctacctgtgg cccgactgga aatacctcac gctcgaacgc actgccgccc
     6961 ttgccgtatg cagcaacggc gcctatctcg aaaaaatcgc cgaacgcctc aaaagcggcg
     7021 aaacggccga cgacgtcgaa taccgcgcca cccgccgccg cgcccacgaa cacaccgccg
     7081 ccctcagcag caccctttcc gacatgagca gcgaacccgc aaaattcgcc gacagcctgc
     7141 aacccggctt taccctgctc aaaaccggct acgccctgac cggctacatc tccgccctcg
     7201 gcgcataccg cagcgaaatg cacgaagaat gcagccccga ctttaccgca cagttccacc
     7261 tcgccgccga acacaccgcc cacatcttcc aacacctgcc cgaaaccgaa cccgacgact
     7321 ttcagacagc actggataca ctgcgcggcg aactcgacac cctccgcacc cacagcagcg
     7381 gaacacaaag ccacatcctc ctccaacagc tccaactcat cgctcggcag ctcgaaccct
     7441 actaccgcgc ctaccgacaa attccgcaca ggcagcccca aaacgcagcc tgaaaaagtt
     7501 tcggcatttt gtaagagagg gcagattgtc agacaggttg caagatagtg gataaagctt
     7561 tgcgcagggt aaatgcgtag caactgaacc gtcattccca cgaaaacaga aaaccaaaaa
     7621 cagaaaccta aaatcccgtc atttccacga
//
LOCUS       DAWHXZ010000089         7356 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000089 DAWHXZ010000000
VERSION     DAWHXZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.089"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..311)
                     /locus_tag="WIE35_001197"
     CDS             complement(<1..311)
                     /locus_tag="WIE35_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEZ4154113.1"
                     /translation="MTETQSLELAKELISRPSVTPDDQNCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KT"
     gene            complement(425..1045)
                     /locus_tag="WIE35_001198"
     CDS             complement(425..1045)
                     /locus_tag="WIE35_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenosine tetraphosphatase"
                     /protein_id="HEZ4154114.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     RLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(1079..1588)
                     /locus_tag="WIE35_001199"
     CDS             complement(1079..1588)
                     /locus_tag="WIE35_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ4154115.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(1683..2234)
                     /gene="azu"
                     /locus_tag="WIE35_001200"
     CDS             complement(1683..2234)
                     /gene="azu"
                     /locus_tag="WIE35_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ4154116.1"
                     /translation="MKTYLALISAAVIGLAACSQEPAAPAAEATHAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(2515..2937)
                     /locus_tag="WIE35_001201"
     CDS             complement(2515..2937)
                     /locus_tag="WIE35_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ4154117.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLAPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            3068..5818
                     /gene="secA"
                     /locus_tag="WIE35_001202"
     CDS             3068..5818
                     /gene="secA"
                     /locus_tag="WIE35_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ4154118.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHTLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPIQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            5962..>7356
                     /locus_tag="WIE35_001203"
     CDS             5962..>7356
                     /locus_tag="WIE35_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ4154119.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPTAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLL"
BASE COUNT         1834 a         2034 c         1854 g         1634 t
ORIGIN      
        1 gttttcatgt ccgccgcacc gcgcccgtat aatcttccgt cgcgctcggc cggctcgaac
       61 gggggcgaat cccatttttc gacagggcct gtcggtacaa cgtcggtatg ccctgcaaaa
      121 cagacgacgg gagctttcgt gccgcgtcgc aaccagatgt ttttggtgtc gccgaaatgg
      181 agttcttcag ccgcaaaacc gattttgtgc aggcgttcgg caaggagttt ttggcaattt
      241 tggtcgtcgg gggtaacgga tgggcgggaa atcaattctt tggcgagttc tagggattgg
      301 gtttcggtca tatttgttca cttttgaaat tagaccatct gaaacgttct gaatgtgatt
      361 ttcagacggc atttaggtta ggttggcata cggggtgggt atttttaccc gtcagtcttc
      421 tgaatcattt gccgtgacac gcttcgtaaa gcggcagcaa atcttccacc gtttccgcta
      481 tccatttcgc gacatcctgc ctgcccaaat cgtcgcgttc gatgtgtttg ccgatgcaga
      541 agaaatcttc gtcgttttgc aaccttcggt cggattcatt ttgctgcgcg acagtgcggt
      601 agtcgtcgta ttcgctttcc gcgccgtgcc acatatcgaa agaagcgtat ttttcagtat
      661 caaaattatc caaccaacgg ttgtaatcag gcagcgcaat cggcgacacg tcggctttgt
      721 agcaatgcca gtccaagctg acactcaaac ggcggcggtt gagcagtatc gacaaaatcg
      781 cggcggaatt tttatattgt tcgtatttga agtaggcaaa gaaatgggcg cgaacctgcc
      841 agccgttgca ccagcgttcg atgtgcggcg gcgcaaacgg cgcacccaat tcggcggcaa
      901 cctgctgaat cagctgctgc catatctgcc agttttcctt gtagtcggct ttgatttgcg
      961 gaatgctttc aggctggtat tttttaagct gggcaaactg gaaaaacggg atattgaaca
     1021 aatcgcaact tttcggggtc agcataatat atccttgaga cgattgtttc agacggcatt
     1081 atttgcgccg gcgcgccgcc ataatttcgc cgatttcggt cagtttttct tttgggataa
     1141 aggtgttgcc catatcaaac agcggctctt caatcgccaa atgaacatca tatcccgcca
     1201 caaaacgttt gaacgcttcc tcatcgggga cataagcgtt gtctgcttcg agtttggcaa
     1261 attcggcgga aacagccgcc cagttgtcgt gcagcccgat atgttggcgc aaaagctcgt
     1321 ccacgctttc ttgggcttgc ggcgcatatt gcagcagcag cgggaagaag ttttcttctt
     1381 cgtcttcatg gtgcagcggc gcggcaacgt tgaaatactg ggcgatttgg cggatggttt
     1441 gcaaaacaat ctgattgcag ccgttttcgg cgatatagtc cgacagcatg gcgacttgtc
     1501 cgcaaaaacg gcgcactttg ccgtggcagg catacagcat ttcaatcggt tcggcaaagg
     1561 taacgctttt ggtttcaaac ggattcatgt tttcgttctc aacgggcact tttcaagcag
     1621 tcattttata ataaaacagc ctgcacaaag caggctgtcc gtcttttgag actttaagcg
     1681 gattaatcga ccaaagtcac tttgccgttc atcaaagcac cgtgacctgg gaaggtacaa
     1741 gcgaatttgt attcgccgtc agccagtttg gcaggatcca aagtcaggga agactcttcg
     1801 ccgccgccga tcagtttggt gtgggcaaca acgcgcgcat cgtcaggttt gacatagtcg
     1861 gtatcggcag cacctacgcc gtctttaaat acgccgtcca tgtcttcagc tttggcaatc
     1921 acaaggttgt gacccatgct ggctttgggt tgcgtaccgg tatgtttcag agtgatggtg
     1981 aactctttac atgctttgct gacttggatg tctttggtgt tgaactgcat attgtcgttg
     2041 gattcgacag ttgccgcaca gttgccggca gcaggggctt cggcagcatc tgcaggagca
     2101 gcttcggcgg caggtgcttc ggaagcgggt gcttcagcag catgagttgc ctcggcagca
     2161 ggcgcggcag gttcttgaga gcaggcagcc aaaccgataa cggcagcaga aatcagagcc
     2221 agatacgttt tcataacaaa tctccaatcg ataaaataat attcggtttt acggaaatca
     2281 aagtgcaacc gccattaaca aaaccttgaa aaagattccg ccgcgttgca caaacagatg
     2341 tttcggggcg gcattttgct acaaatttca tttgaaatca aagcctgtct gcaagtttac
     2401 aatcgtttac tttaaaaggg gcaattttac cccgaaccta tttctttagt attagaccca
     2461 ttatccttta cttcttaata ttaacggatg tttacacaaa ttcccgtata cattttatgc
     2521 gccatgcctt ctaaccaagt ttgccaatgc ctccgccaat tcgggatgcc gtttttccaa
     2581 ctttaccgcc gccgaaccga aactctccag cgcagcctta ctcaaatgca gggtattggt
     2641 tttcggcggt ttttccggtt tcggggccag cctgaccgaa acagagcgta tcgaagcatc
     2701 aagccctgcc aactgcggca ataccgacgg tgcaatcatt ttcaagcgcg atgccgccat
     2761 attgtttgcc gccaaaagga caagcctgcc gtcttcgata catgccgtct gaaaatgcgg
     2821 gtgcaggttg gcaggcagca gttttttcac ggcggcatcc aaccgccgcc actgtcccgc
     2881 ctgtttcaaa agtccggaaa gcagcgcgtc ccgcctgccc aactgttcca aattcataaa
     2941 acatacaccc aaaaagattg aaataccgca aacgcgcctt tatttcagac ggcattagca
     3001 ctttgcacaa acgcttgtgt taaaatcgcg ttttcgccca ctattatatc aggcgcagga
     3061 attattcatg ctgacaaaca ttgccaagaa aatcttcggc agccgcaacg accgcttgct
     3121 gaaacaatac cgtaaatccg ttgccagaat caacgcgctc gaagaacaga tgcaagccct
     3181 aagcgatgct gatctgcaag ccaaaactgc cgaattcaaa caacgcctcg ccgacggtca
     3241 gactttggac ggcattttgc ccgaagcctt cgccgtctgc cgcgaagcgt cccgccgcac
     3301 cctcggtatg cgccacttcg acgtgcagct tatcggcggt atggtgctgc atgacggcaa
     3361 aatcgccgaa atgcgtaccg gcgaaggcaa aaccctggtc gccaccctcg ccgtctatct
     3421 caacgcgctg gccggcaaag gcgtacacgt cgttaccgtc aacgactacc tcgcctcacg
     3481 cgatgcgggc atcatggaac cgctctataa tttcctcggc cttaccgtgg gcgtgattat
     3541 ttcagatatg cagccgttcg accgtcaaaa cgcctatgcc gccgatatca cctacggcac
     3601 caataatgaa ttcggcttcg actacctgcg cgacaatatg gttaccgacc aatacgacaa
     3661 agtgcagcgc gaattgaatt ttgccgttgt cgatgaagtg gattccatct tgattgacga
     3721 agcgcgcact ccgctgatta tctccggcca ggcagacgac aacatccagt tgtaccaaat
     3781 catgaacacc gtcccgcccc acctcgtccg ccaagagaca gaagaaggcg aaggcgacta
     3841 ctgggtagat gaaaaggcgc accaggtcat cctgagcgaa gccggccacg aacacgccga
     3901 gcagattctg acccaaatgg gattgctggc agaaaacgac tcgctctatt ctgccgccaa
     3961 catcgccctg atgcaccacc ttatggcggc attgcgcgcg catacgttat tccacaaaga
     4021 ccaacattac gttatccaag acggcgaaat cgtcatcgtt gacgaattta caggccgtct
     4081 gatgtccggc cgccgctggt ccgaaggcct gcatcaagcc gtggaagcca aagaaggcgt
     4141 ggaaatcaaa cgcgaaaacc aaaccctcgc ctccatcacc ttccaaaact atttccgcct
     4201 gtacaccaag ctctccggca tgaccggcac agccgatacc gaagccttcg agttccaaag
     4261 catctacaac ctcgaaaccg tcatcatccc gaccaaccgc cccatacaac gcaaagactt
     4321 caacgaccag attttccgtt ccgccgaaga aaaattcgaa gccgtcgtca aagacatcga
     4381 agaatgccac aaacgtgggc agcccgtcct cgtcggcaca accagcatcg aaaactccga
     4441 actggtctcc catcttttgc aaaaagccgg tctaccgcac aacgtcctca acgccaaaga
     4501 acacgaacgc gaagccctga ttgtcgccca agctggcaaa gtcggcgcga ttaccgttgc
     4561 caccaatatg gcgggacgcg gtacggacat cgttttaggc ggcaacctga agcaccaaac
     4621 cgatgccatc cgtgccgacg aaaccttgag cgacgaagag aaacaggcac aaatcgccgc
     4681 actcgaaaac ggctggcagg cagaacacga taaagtaatg gaagcaggcg gcctgcacat
     4741 catcggtacg gaacgccacg aaagccgccg tatcgacaac caattgcgcg gacgttccgg
     4801 ccgtcagggc gaccccggat ccagccgttt ctacctttcg tttgaagacc cgctgctgcg
     4861 cttattcgca ctcgaccgcg ccgccgccat cctcaaccgt ctcgcccccg aacgcggcgt
     4921 tgccatcgaa cacaacctgc tgacgcgcca aatcgaaggg gcgcaacgca aagtcgaagg
     4981 cagaaacttc gatatgcgca aacaggtttt ggaatacgac gacgttgcca acgaacagcg
     5041 caaagtcatt tacagccagc gcaacgaaat tctgaccagc aaagacatca gcgacctgat
     5101 gcaggaaatc cgttctgatg tcgtcagcga cctcgtggat acctatatgc cgcccgacag
     5161 catggaagaa caatgggaca tcccgacttt ggagaaccgt ctggctgccg aattcagact
     5221 gcacgaagac atccaatcct ggctgaaggc ggacaatgcg attgacggtc aagacatcaa
     5281 agaacgcctg atcgaacgca tcgaaaacga atatgccgcc aaaaccgaac tggtcggcaa
     5341 gcaggcaatg gccgatttcg agcgcaacgt gatgttgcag gtcatcgaca accaatggcg
     5401 cgaacacctc gccgctatgg actacctgcg acaaggcata cacctgcgca gctatgccca
     5461 aaaaaatccg aagcaggaat ataaacgtga agcctttacc atgttccaag acctgtggaa
     5521 cggcatcaaa ttccatattg cctccctgct tacctcggtt caaatcgaac aaaaccctgt
     5581 cgcggtggtt gaagagcaac ccatcggcaa catccagtcc atccattccg aatcgcccga
     5641 tatggaagaa cttttgggtc agtcgcaaac cgatctggtt accgaagcct ttaatcccga
     5701 tgggacggat ttcagccccg aagccttgga agcgcgggga caaatcgtcc accgcaacga
     5761 cccctgcccc tgcggcagcg gtttgaaata caaacaatgc cacggcaaac tggcttgagc
     5821 gtttgaacgc aaatgccgtc tgaacatccg gtttccggct tcagacggca ttttgcctga
     5881 accgccacat ccgaccgcca ttccgaaaaa tcccgatttc gtaccgtcca aactaaaaac
     5941 aggcatccgc cccgccgaac tatgattcca tccgacttca ttgacgaact tctggccaaa
     6001 accgatattg tcgacatcat cgacgaacag gttccgctga aaaaaggcgg ggcgaactat
     6061 atggcgtgtt gcccgttcca caaggaaaaa acgccgtcgt tttcggtcag tccgaccaag
     6121 cagttttacc actgtttcag ttgcggggcg cacggttcgg cgattggttt tgtgatggaa
     6181 catcaggggc tgtcgtttcc ggaggcagtg cagttccttg ccgaccgcgt gggtatgatt
     6241 gtgcctaaag tgcgcgggca gaacgataat cccgaagtcc gtgccgaacg taagaaaaag
     6301 cagcagacgc tggaagaaac gacagcggcg gcggctgatt tttatgcgca acagttgaaa
     6361 ttcaatccga cggcgaaggc ttatttggac aagcgcggct tgagcgcgga agtcatcgcg
     6421 cattatggtt tgggctacgc gcccgacggc tggcagcctt tggcgcaagt attccaaccg
     6481 tacccgaata ccgcgttggt ggatacgggt atggtgattg acaatgaggg gcggtattac
     6541 gaccgattcc gtcatcggat tatgttcccc atccgcaatc cgcgcgggca ggtcatcggt
     6601 ttcggcggca gggtactgga cgactccaaa cccaaatatc taaactctcc cgatacgcct
     6661 ttgtttgata aggggaaaaa cctttacggc ttgtatgagg ggcgtgccgc tgtcaaggaa
     6721 gcggagcgga ttctggtggt cgaaggctat atggacgtgg tcgcgctggc acagttcggc
     6781 gtgggctacg gtgtggcggc cttgggcacg gcaacaacag ctgaacacgt caaaatccta
     6841 atgcgtcagg cggacagtat ttatttctgt ttcgacggcg acagcgcggg gcgcaaagcg
     6901 gcttggcgcg cgctggaaaa cgcgctgccg cagttgaagg acgacaaatc gctgcatttt
     6961 ttgttcctgc cggaagaaca cgaccccgac agttacatcc gtgcctacgg caaagcgcaa
     7021 tttgaagacg cgcttctgaa tcaaagcaag cccttgtcgg agtatttctg ggaacacctt
     7081 tcagacggca ttcatctcaa tacgcaggaa ggcaaggcgg aattggtaaa aaccagttcg
     7141 ccgcttttgg cgcagattac cgcgccggca ttggcttatt tgttaaaaca acggcttagc
     7201 gaactggtcg gcatcgaccc cgacaacctc gcgcaactgc tgggacagga agcgccgaag
     7261 cggcacgtca aacaaaaaaa ctacaaactg ccgccgattt ccgtcaaaca gcccgttatg
     7321 ccgacattgg tgcaacggca aatccgcagc ctcttg
//
LOCUS       DAWHXZ010000090         7287 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000090 DAWHXZ010000000
VERSION     DAWHXZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.090"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(91..1371)
                     /locus_tag="WIE35_001204"
     CDS             complement(91..1371)
                     /locus_tag="WIE35_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ4154120.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(1593..2213)
                     /locus_tag="WIE35_001205"
     CDS             complement(1593..2213)
                     /locus_tag="WIE35_001205"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ4154121.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQSREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNNIEKVLDGHFGC"
     gene            complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="WIE35_001206"
     CDS             complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="WIE35_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ4154122.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQIAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            3352..3924
                     /gene="ampD"
                     /locus_tag="WIE35_001207"
     CDS             3352..3924
                     /gene="ampD"
                     /locus_tag="WIE35_001207"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ4154123.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            4118..5404
                     /locus_tag="WIE35_001208"
     CDS             4118..5404
                     /locus_tag="WIE35_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ4154124.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKLQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            5552..>7287
                     /gene="ligA"
                     /locus_tag="WIE35_001209"
     CDS             5552..>7287
                     /gene="ligA"
                     /locus_tag="WIE35_001209"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ4154125.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHICRLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARRQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEHMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTVVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAEA
                     PAANQTQSVPLYKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQP"
BASE COUNT         1693 a         2159 c         1834 g         1601 t
ORIGIN      
        1 ttgtcctgat ttttgttaat ccactatatc aatctgacag caaaatgccg cctgaaaaca
       61 gttcagacgg cattttaaac cgcaaacggc ttacagcttc cattcgctca gcttggcagc
      121 gtaagcttcc aaatctgcaa tcggacgggt tgccacgccg ctttccatcg ctgctttggc
      181 ggcagccgta gcaacgcgcg gcagcaggcg ggaatcgaac ggagtcggaa tcaggtattc
      241 cgcgccgaat tcaaatttct taccgtaagc ggcaaccact tcttcggtta cttcttccat
      301 cgccaaatcc gccaaagcat acacgcaggc gcgtttcatt tcttcgttga tggtcgtcgc
      361 gccgacatcc aacgcaccgc ggaagatgaa cgggaagcac aatacattgt tcacttggtt
      421 cgggaagtcg gagcggccgg taccgataac cacgtccgga cgggtttctt tcgccagcgg
      481 cggcaggatt tccggattcg ggttggccat ggcgaacacg atgggttttt cgttcatcgt
      541 gttcaacatt tcgggggtca gcaggttcgc gccggagagg cccaagaaga tgtctttacc
      601 tttgacggca tcggcaagta cgcgctggcc attgtcttca acggcgtaga attttttgga
      661 ttcgtccatg cggtctttgt cttcgcgggt ttggtaaatc acgcctttgg agtcgcaaac
      721 ggtcacgttt tcgcgtttca agcccaaatc cagcaattgg ttcaagcagg caatcgcggc
      781 cgcacctgcg ccggaacaca ccaaagtcgc ttcttcgatt ttacggccgg taaaacgcag
      841 ggcgttcaat acggcggcgg cggtaatgat ggccgtgccg tgctggtcgt cgtggaatac
      901 ggggattttg cagcgtttgc gtaattcgcg ttcgatgtag aaacactcgg gtgctttgat
      961 gtcttcgagg ttgatgccgc cgaaggtcgg ctctaaagcg gcgatgatgt ccacgagttt
     1021 ttgcgggtct ttttcatcga tttcgatgtc gaacacgtcc acaccggcga attttttgaa
     1081 caatacgcct ttaccttcca tcacgggttt gcccgccagc gcgccgatgt cgcccaagcc
     1141 caaaacggcc gtaccgttgg aaatgacggc gaccaagttg cctttggcgg tgtatttgta
     1201 ggcattttgc ggatcggcat ggatttccat acaaggagcg gctacgcccg gagagtacgc
     1261 caacgccaaa tctttgtcgg tcgccaaaga tttggtcggg gtaacggaaa ttttgcccgg
     1321 aacgggtaat tcgtggaact tgagggcggc ttctttcaat gagttttcca tgtgtcaatc
     1381 ctgttgcgag aaaaagattt gaatcggact gtccgaaacg ctttcgggtc atcatgttac
     1441 gggcggttcg ttaatattgc cgacgcatcc tgccttgtaa ccgagcgtaa ttctatcatg
     1501 ccgaaagttt ggcgcatacc gccattttca cctgcggcac tgccgtttga ttttcggcgc
     1561 gggtggatgc gcttgtttca atatttgccg cattaacagc cgaaatgtcc gtccaacact
     1621 ttttctatgt tgtttctgac ttcatccaag ctgcggttac tgtcgataat ggcgtaccgt
     1681 tcgggacagg cggcggcacg gttcagataa acgctgcgca cacgcatgaa gaaatctgcc
     1741 tgttcctgct cgaaccggtc tttctcgcgc gactgtccga tacgcgccat cgacacttcc
     1801 agcggcacat ccaacagcag ggtcaaatcg gggcgcaaac cgccctgcac ccaatgttcc
     1861 aaaatttcaa tgtcttcaga cggcatcccc cgcccgccgc cctgataggc gaaggttgcg
     1921 tcggtaaaac ggtcggacac gacatggatg ccgtctgaaa gtgcaggcaa tatcacgtct
     1981 tcaatatgct gcattctggc ggcgaacatc atcagtgttt ccgcacgcaa accggctttg
     2041 gtttcagggt tgagcaggat ttcgcgcaag gcctcaccga ccggcgttcc gcccggttcg
     2101 cgcgtgaaca gcacgggcag ccccctccgt tcaaaccatg ccttgatgac ggcaaggttg
     2161 gtggatttgc cggcaccgtc tatgccgtcc aaagtgatga attgcggttt catgcttgca
     2221 gttccggtaa gggtatgccg tccgaaaaca caaacttttc agacggcatg gtttattttt
     2281 tcaaaatata tttgcgaacg gcggcgttgt gttcggtcaa atcatggctg aactggctca
     2341 agcccgtacc gtccattttg gacacgaaat acaggtattt ttcaccggac ggatgggcgg
     2401 cggcatcgag tgccgccttg ccgggcagcg cgatcggggt tggcggcaga ccgccgcgcg
     2461 tgtaggtgtt gtacggcgtg tcgcggcgca ggtcggcttt acggattttg cccttgtatg
     2521 ccgcacccat gccgtaaatc acggacgggt cggtttgcag gcgcataccg attttcaggc
     2581 ggttgacgaa gacggaagcg acatggtcgc ggtcggcttc atgccctgtt tccttttcga
     2641 tcaggctcgc cataatcagc atttcataag ggtttttata aggcagcccg tcctgcctgc
     2701 tttcccatgc ctcattcagt cggcgttgca tcgccttgta ggcgatttgg taaatccgta
     2761 aatcgctgcc gcccgcatcg atttcgtagc tgtcggggaa aaactgccct tcaggattgc
     2821 cgctgaaggc atcaggggca acttccgcca tcagtttttc attgctccag cctttggtgt
     2881 cgtgttcgat gtcgggcgtt gcgtcgatga ctttcctcat atgcgaaaaa cgcgaacctt
     2941 cgataatctg cacggtaacg gaatccggcc tgccgccgcg cattttctgc aagatatccc
     3001 aagcagacac ttccgaaggc agtctgtacg tccccgtatg cagcctgttg tgcacaccca
     3061 aaacgtaggc cgccgccgtc aaaacatgcc tgctgaacac gatgcggtct tcggcaagtt
     3121 tcctgccgac cgacgaaata ccctggtttt tggcaatttt aatcctgtat gccctgccgt
     3181 tgtctttagg gacgaaaagc agcgcggcga aaacggctgc cgatacggtc aaaaaaacgg
     3241 cagaccattt caacaatttt ctcaacatgg taggattccc agcattcgga atacgtctta
     3301 aaattcgggc gacagtataa cccaaattgc ccgccggaca ggaagaacat catggacaat
     3361 cacgccgaag cacactggca aaacggctgg cttcaaagca tacgccatac cccgtcgccc
     3421 aatttcagcc cgagggaaac gggggaaacg gtttccctga tcgtgttgca caacatttcg
     3481 ctgccgccgt tcgaatatgg tacggatgcg gtggaaaagc tgtttgccaa ccggctcgac
     3541 cccgacggac atccgttctt cagcctgata cacactttgc gcgtatccag ccatttttta
     3601 atcaaacgcg acggcgaaac ggtacagttc gtttcctgcg acaatatggc ataccatgcc
     3661 ggcgtatcct cgtttcgcgg acgggaaaaa tgcaacgcat tttccatcgg catcgaactg
     3721 gaaggctgcg atttcgaacc ctttaccgaa gcgcaatacc gttcgctcga agccttgttg
     3781 gacgcaatct gccgccgcta ccccatcacg gcggtaacgg ggcaccaaga catcgcgccc
     3841 ggccgcaaaa ccgaccccgg tcattttttc gactggcggc ggatacgggg gaaagggttt
     3901 cccgtggaca gaaatgccgt ctgaaaacca cagattttag acggcatttc ggaaaatttc
     3961 cgcttatttc gacacactgc tgtcagaccc tgcaattttc gttataattc aaacgttata
     4021 accggccgtt ccatcagact gccggaaacg caagcccgat ttaaccgcag tacccgcact
     4081 tcgcgcatcc tgcatcccga aatttaaagt gattgccatg atttacatcg tactgttcct
     4141 cgccgccgtc ctcgccgttg tcgcctacaa tatgtatcag gaaaaccaat accgcaaaaa
     4201 agtgcgcgac cagttcggac actccgacaa agatgccctg ctcaacagca aaaccagcca
     4261 tgtccgcgac ggcaaaccgt ccggcgggtc agtcatgatg ccgaaacccc aaccggcggt
     4321 caaaaaaacg gcaaaactcc aagaccctgc tatgcgcaac ctgcaagaac aggatgccgt
     4381 ctacatcgcc aagcagaaac aggcaaaagc ctccccgttc aaaaccgaaa tcgaaaccgc
     4441 cttggaagaa agcggcatta tcggcaactc cgcccacacc gtttccgaac cccaaaccgg
     4501 acattccgca ccgaaacctg ccgacgcgcc ggcaaaacct gcacccgttc cgcaaacacc
     4561 tgcaaaaccg ctgattacgc tcaaagaact gtcaaaagtc gaattaccct ggtttgacgt
     4621 gcgcttcgac ttcatctcct atatcgcgct gaccgaagcc aaagaactgc acgcactgcc
     4681 acgcctttcc aaccgctgcc gctaccagat tgtcggctgc accatggacg accatttcca
     4741 gattgccgaa cccatcccgg gcatccgcta tcaggcattt atcgtgggta ttcaggcagt
     4801 cagccgcaac ggacttgcct cgcaggaaga actctccgca ttcaaccgcc aggtggacgc
     4861 attcgcacaa agcatgggcg gtcagacgct gcacaccgac cttgccgcct ttatcgaagt
     4921 ggcttccgca ctggacgcat tctgcgcgcg cgtcgaccag accatcgcca tccatttggt
     4981 ttccccgacc agcatcagcg gcgtagaact gcgttccgcc gtaacgggcg tgggtttcgt
     5041 tttggaagac gacggcgcgt tccactatac cgacacgtcg ggctcgacca tgttctccat
     5101 ctgctcgctc aacaacgagc cgtttaccaa cgcccttttg gacaaccagt cctacaaagg
     5161 cttcagtatg ctgctcgaca tcccgcactc tccggcaggc gaaaaaacct tcgacgattt
     5221 gtttatggat ttggcggtac gcctgtccgg ccagttgaac ctgaatctgg tcaacgacaa
     5281 aatggaagaa gtttcgaccc aatggctcaa agacgtgcgc acttatgtat tggcgcgtca
     5341 gtccgagatg ctcaaagtcg gtatcgaacc gggcggcaaa accgcattgc gcctgttctc
     5401 ctaaaatcga atgcaatcaa agcggatatg tcgttacggc atatccgctt tttttcgggt
     5461 aaaattcagc tgttttcaac ctatcatttt tcagacgacc tttgcccgtt tcatcccgta
     5521 tcagacaaag gtcgtctgaa aaccttctct catgaatcca aactcaaaac ataacactaa
     5581 ctttaccaat cttcttaaac cgtctgatag tgatataaaa caatttgccg cacaacacat
     5641 ctgccgcctc accgacctcc tcaaccgcta cgcctacgaa tactacaccc tcgacgcgcc
     5701 cagcgtaccc gatgccgaat acgacaaatt gttccgcgaa ctcgaagcgt tggagttaaa
     5761 ccatcccgag ctcaaactgc ccgacagccc gacccagcgc gtcggcggcg agcctttggc
     5821 gggatttgcc gaagtacgcc acgaagtgcc gatgctgtcg ctgaccaacg ctttttcccc
     5881 gcaagatgaa aacggcgtgt tcgaccatgc cgaaatgtac gccttcgacc aacgcgtgcg
     5941 cgacggcttg gacggcggca atcccgaata cgttatcgaa cccaaattcg acggcctcgc
     6001 catcagcctg ctctaccgcg acggcgtatt ggtgcaggcg gcaacgcgcg gcgacggcac
     6061 gacgggcgaa gacgttaccc aaaacatcaa aaccgtatcc aacatcccct tgcggctgca
     6121 cggcgaaaat acgcccgaac tcatcgaagt acgcggcgaa gtactgatgc aaaaagccga
     6181 ttttgccgcc ctcaataaaa gacaagccga aaacgggcaa aaaccctttg ccaatccgcg
     6241 caacgccgcc gccggcagcc tacgccaact cgattcgcgc atcactgcgc aacgcaaact
     6301 gcactttttc ccctactccg tcgcccgccg gcaaggcggt ttcgtcgcgg aagaacacat
     6361 ccaagaactc gcctatttcc aagcactcgg cttcagtctg cccaatggca atttcggctg
     6421 tttcaaaaat atcgacgaag tattggcgtt ttacgaacac atgcagcaaa aacgccccga
     6481 actgccctac gaaatcgacg gcatggtggt taaagtcaac agcctggcgc aacagcgcga
     6541 actcggcttc atctcccgcg cgccgcgctg ggcgattgcc cacaagttcc ctgccgaaga
     6601 agccctgacc gtcgtcgaag cgattgacgt gcaaatcggc aggacgggcg cggtaacgcc
     6661 ggttgcccga ttacaacccg tattcgtcgg cggcgtaacc gttaccaacg ccaccctgca
     6721 caatcaggac gaagtttccc gaaaagacgt gcgcgtcggc gataccgtcg tcgtgcgccg
     6781 cgccggagac gtgattcccg aagtcgtgcg tgtgattttt gaacgccgcc cgatgcagga
     6841 aaccgccgtt gctgtttcag acggcatcgg gcatcggcag gacgatttgt ttgccgaagc
     6901 accggctgca aaccaaaccc aatccgttcc gctctacaag ccctaccgcc tgccgaccca
     6961 ttgccccatc tgccgcagcg aaatcgaacg cgaagaaggc gaagccgtcg cccgatgcag
     7021 cggcggtatg ctttgtcagg cacaacgcgc gcaaggctta atccacttcg cctcgcgcaa
     7081 agccatggac atcgacggct tgggtgaaaa acaaatcgaa cagcttgtcg cccaagacct
     7141 cgtccgccac ttcgccgacc tctaccgcct cgatattccg accctgcaaa aaatgaagga
     7201 aacggcggac aaaaccgttg ccgaatcgga tcaaatgccg tctgaaggca gttcggtcgg
     7261 tgcgtcgggc aaacataaaa aacaacc
//
LOCUS       DAWHXZ010000091         7270 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000091 DAWHXZ010000000
VERSION     DAWHXZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.091"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            97..237
                     /locus_tag="WIE35_001210"
     CDS             97..237
                     /locus_tag="WIE35_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEZ4154126.1"
                     /translation="MISNHDTEFTRDIYSFAILKTIEVQRNIAAKGSSRKKVGELLAI
                     YD"
     gene            230..1039
                     /locus_tag="WIE35_001211"
     CDS             230..1039
                     /locus_tag="WIE35_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ4154127.1"
                     /translation="MIDFDKPAEEDAIYQSRLKKIVSDDLVLYNENSLNVMRKILEKY
                     PNGCFDMIFADPPYFLSNDGFSCQNGQMVSVNKGNWDKSKGMAADLEFYEEWLRLCYA
                     LLKPNGTIWVCGTFHNIYLIGYLMQTIGYHILNNITWEKPNPPPNLSCRFFTHSTETI
                     LWAKKNKKAKHTFHYEMMKAQNDGKQMKCVWTFPPPNKAEKTFGKHPTQKPLPLLERC
                     ILSASNIGDLIFDPFMGSGTTGVAALKHGRKFCGCELEEDFFELAKKRLEK"
     gene            1036..1590
                     /locus_tag="WIE35_001212"
     CDS             1036..1590
                     /locus_tag="WIE35_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154128.1"
                     /translation="MIIGGIGGARTQTGLRFEERTDLRKLFEEIPGYDLRKTYDNAGY
                     EVWFNGELKAYCFKKYEFYRFLKRPGYSINWKDHLSKRLLPDNGLFIIIRDTLFIIEI
                     KFQQTPGSVDEKLQTCDFKRKQYTKLVHSLGWRVEYVYVLNDWFTKPEYKDVLDYIIS
                     VNCHYQFNTIPLRWFGLPDGETNE"
     gene            complement(2465..2776)
                     /locus_tag="WIE35_001213"
     CDS             complement(2465..2776)
                     /locus_tag="WIE35_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ4154129.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(2773..3140)
                     /locus_tag="WIE35_001214"
                     /pseudo
     CDS             complement(2773..3140)
                     /locus_tag="WIE35_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3968..4053)
                     /locus_tag="WIE35_001215"
     tRNA            complement(3968..4053)
                     /locus_tag="WIE35_001215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4017..4019),aa:Leu,seq:gag)
     gene            complement(4067..4417)
                     /gene="secG"
                     /locus_tag="WIE35_001216"
     CDS             complement(4067..4417)
                     /gene="secG"
                     /locus_tag="WIE35_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ4154130.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            complement(4424..5197)
                     /locus_tag="WIE35_001217"
     CDS             complement(4424..5197)
                     /locus_tag="WIE35_001217"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ4154131.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            5381..5884
                     /locus_tag="WIE35_001218"
     CDS             5381..5884
                     /locus_tag="WIE35_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ4154132.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAA"
     gene            5986..6642
                     /locus_tag="WIE35_001219"
     CDS             5986..6642
                     /locus_tag="WIE35_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ4154133.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALEEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            6721..7044
                     /locus_tag="WIE35_001220"
     CDS             6721..7044
                     /locus_tag="WIE35_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ4154134.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
BASE COUNT         1705 a         1588 c         1933 g         2044 t
ORIGIN      
        1 tcatttacca cttatgccaa agaaggtttc aatttagaca atcaaatccg tcttgcccag
       61 tcagcccaac aaattgcgcc cgattcccaa ggcgttttga tttcaaatca tgatactgaa
      121 ttcacaaggg atatttattc ttttgctatt ctgaaaacaa ttgaagtaca acgcaatatt
      181 gccgcaaaag gcagcagccg taaaaaagtc ggagaattat tggcaattta tgattgattt
      241 tgacaaaccg gctgaagaag acgccattta tcaaagtcgt ttgaaaaaaa tcgtttcaga
      301 cgaccttgtc ctctacaacg aaaattccct taacgtcatg cggaagatat tggaaaaata
      361 tccaaacggc tgttttgata tgattttcgc agacccgcct tactttcttt ccaatgacgg
      421 cttcagttgt caaaacggac aaatggtttc cgtcaataag ggcaactggg ataaatccaa
      481 aggaatggcg gcagatttgg aattctacga agaatggctc cgactgtgtt acgccttatt
      541 aaaaccaaac ggcacaattt gggtttgcgg cacattccat aatatttatt taatcggcta
      601 cctgatgcaa actatcggat accatatttt gaacaatatt acttgggaaa aacccaatcc
      661 tccccctaat ttgtcctgcc gtttttttac ccattcgaca gaaacaatct tatgggcaaa
      721 gaaaaataaa aaagccaaac acacgtttca ttatgaaatg atgaaagcac aaaatgatgg
      781 caaacagatg aaatgtgttt ggacatttcc tcctccaaac aaagccgaaa aaacattcgg
      841 caaacatccg acacaaaaac cactcccctt gcttgaacgc tgcatactct cagcttcaaa
      901 catcggagat ttaatttttg accctttcat gggcagcggc acaacaggcg ttgccgcctt
      961 aaaacatggt cggaaatttt gtggttgcga actggaagaa gatttttttg aattagcaaa
     1021 aaaaaggtta gaaaaatgat tattggcgga atcggtggtg caaggacaca aacaggactc
     1081 agattcgaag aacgtacaga cttacgaaag ttgtttgaag aaattcccgg gtacgactta
     1141 agaaaaacat atgataatgc gggttatgaa gtttggttca atggagaatt gaaggcttat
     1201 tgttttaaaa aatatgagtt ttaccgattt ttgaaaagac cgggatacag tattaattgg
     1261 aaagaccatc tgtctaaaag attactaccc gacaatggct tatttatcat catccgtgat
     1321 accttattta ttattgagat caaattccaa caaactcccg gttcagtaga tgagaaatta
     1381 caaacttgcg attttaagag aaaacaatat acaaaattgg ttcactcttt aggttggcgg
     1441 gttgaatatg tctatgtttt gaatgattgg tttacaaagc cggaatataa agatgtttta
     1501 gattatatta tcagtgttaa ctgccattac caatttaaca cgattccctt aaggtggttt
     1561 ggactgcctg atggtgaaac aaatgaataa ataccatatt taaaacacag ccgttcaagg
     1621 tcgtctgaat atcatttcag acgacctttc accaatcccc gccgttttcc atctttctgc
     1681 tattgtgata aagtagcccg accttttgtt caaaatacgc ggattccgcg acactgaatg
     1741 cagcatctgt caaagattat ttgaaaacgg tcgagttaga caagtctgcc gacaatgtcg
     1801 ataccacatc caaaactatc cgcagggttt aggcggtatt ccagagtacg gtgtcttatg
     1861 ctgatgctgt aaacaatgcc cgtatagaaa atctaaaacg gttgttgccg accgttatgc
     1921 cggtttatga gcaaagtgtc agaaataagg ggcgcgttaa tatagtggat taaatttaaa
     1981 tcaggacaag gcgacgaagc cgcagacagt acagatagta cggcaaggcg aggcaacgct
     2041 gtactggttt ttgttaatcc actataaaaa acgtcggcgt taagggaagg ggatcgagaa
     2101 tttgagccgt tgtttcaaaa tgccgtctga aatcgtttga gattcagacg gcatttaaac
     2161 agttctgcgc ccgcccgttt tgccttcaag cccgcgccgc ttaaatccaa tcccccctgc
     2221 aacgggcgca aaaaaatctc cgtgcctcgc ggtcggggat gtttcgggtt tcacgcccgt
     2281 ctgcgccgtg ccttgctgtc gggctatgcc gtctgcaccg ccgccgtttc ggcttggttt
     2341 gatgcggtcg ggcatttatg cggctacgcc ctgatattca gttcggcggt cttgcctgtt
     2401 ttggcaggca tattcagcag cgcgtctatt gtgaacttgc tgattttgcc gtttgccaag
     2461 tcggctacgc aatggcttcg atacccttgt ttttgacgat ggtcagcagc ttcaacgccg
     2521 cgccgctggg ctttttaacg ccccgctccc aagccgaaac gtgatttttt cccacgttga
     2581 gatagatggc gaaagcggct tgcgatagtg cctccttctc cctgattgcc ttgatgtctc
     2641 cgccgctcaa cggtttgatt tcggtcaggc aggacttgtc aaagccgcgc atagtctttt
     2701 tgtcgattgc gccgatgttg taagcccctt ccatcatttc atgaatggcg gctaatgcct
     2761 cgcttttgta tttcatgatt ctatctccgt gataatgccg tcttctttca aagccgccag
     2821 ctctgcccgc gtgtatttca gataatatgc ggcggctttt cggtaaacgt ccagttcttt
     2881 atccgaaatg ttttccctgt cgttcttggc aaaggcgtaa acgaaaatgc cctatctgcc
     2941 tgtttgaaca atatcaggct gcgatagccg ccgcttctgc cttgtccttg ccttgctatg
     3001 cgctgcttaa tcacaccgcc gcctaaatct gcgtctatca gcccgttatc cgcccgctct
     3061 accgcttcca gcagctcgga atcgttgatt ttgtgcttct tggcaaattt cactatccat
     3121 tggtttttga atatccgcat tgtgtagccg ttgattagtg atacttggaa ttataccaga
     3181 gacagacaac acggcaagct gcctttttta tgccccgcaa tttctccgtt tatgccgcct
     3241 gaaacactgg cggctaacct gattattttt taatcaatcg cttttaatgg ctcaaattgc
     3301 gtttttagcg cgtttcgctg tcttcctatg tccccatata taaccaagtg gtttaaccgc
     3361 cattcctgct caaaataggc gcggttccgt gcgttttggc ttttcattcc gctacgctcc
     3421 cactgccaag acggtgaaaa accggaaaac cccaaaagtt gttaccgatt cgttaccatt
     3481 tgttaccgca tttgttaccg catttgttac cgcatttgtt accaattttt tatctttttc
     3541 ttattttata gtgaattaaa tttaaaccgg tacggcaagg cgaggcaacg ccgtaccggt
     3601 ttaaatttaa tccactatag tataggtttt ttccaaaaac ccaaaaaaaa accgctctgc
     3661 atcttggacg gtttccccct cattccctac ggtgcgtcag tattccccgc aagctatttc
     3721 ccgcttggca ttcctgattt ggtggtcgtt cagctttatt tttacggtat tttctcgcgg
     3781 tattttcgga tgctgtaaaa aatcaggtta gttcaattag ggtggattgg acgggattgt
     3841 acggtcagga agggggacgg caggataaca atcagcctga aagccttgta ttgattggga
     3901 ttggttgacg gcattagaca ggattaaacg aaaaaaaacg cttagaattt ctaagcgttt
     3961 tgtgtgttgg tgccgacagc gagatttgaa ctcgcacagc ctacggccac taccccctca
     4021 agatagcgtg tctaccaatt tcaccatgtc ggcatttgaa aaactgttat ttctgctgct
     4081 gaggaacagg ggcagaaggt tcggtattgc ttacgggttt gggtgcttgc tgagtttgtt
     4141 gtacgttgct gaagtccaaa ccgtgttttg ttgtgtgggt gtgaatatac accatagcca
     4201 tgcaggttgc aaagaaaaat gttgctgcaa cggcggtcga gcggctgagg aagttagcgt
     4261 tgccggcaga gccgaatacg ccttgcgcgc tgccgcttcc cgatccgaaa gtcgcgccgg
     4321 catccgcgcc tttgccgtgt tggagcaata ctaacacgat gacggccaaa gcggaaatta
     4381 tattaacaat ccaaattagg gttttgaagg cttccatatt tttctacgca ttttgtgcgg
     4441 cactgatgat ggcggtaaag gagtcgtacg acaatgacgc gccgccgacg agtgcgccgt
     4501 ccacataagg tactgcgaag atgtcggccg cgttgtccgc tttcacactt ccgccgtaaa
     4561 ggacgcggat tttaacatcg cttccgcaca aagacaagat ttctttgtag atgaatgcgt
     4621 gcatatcggc aatctgttcg acggtggcga ctttgccggt gccgatcgcc cagacgggtt
     4681 cgtaggcgac ggcgatgttt ttggtatcca gcccttgcag gatggaaagc tgatgggcga
     4741 tgacttcgtg ttctttgccg gcttcgcgct cttcgaggct ttcgccgacg cacaataacg
     4801 ggatgagtcc gacgttgagg acgttttcca ttttgcggcg ttggatttcg tttttttcgc
     4861 cgaaataaag gctgcgttcg gagtgtccga tgaggacgat gtctgtgccg gtgtcggcga
     4921 gcatttcggc ggacacttcg ccggtgtacg cgccgttgtc ggggaagcgg ctcacgtctt
     4981 gggcgcaggt gaggatgcgg ttgtttaaga cgatttgcat ggcgttgtgc agttgcagca
     5041 ggtaaacggt cggggcggcg agtccgatga ggacgcgttc ggcggtgggg aggatgcgga
     5101 agcggtgcat gagcgaattg ttgttctgga gccggccgtt cattttccag ttgccgatga
     5161 cccatttttg atcccacatt ccgatttggt gatacatctt ttttgctccg tgtcgtgttt
     5221 tttctgtctg ccgcgtgtgg cgcgttgcaa tgtgaagttt agtggatatg cggcgggttc
     5281 gcaacttggg gcgggcggct gcgggggcgg tttggaatgt tgtttcgggc aggttgtttt
     5341 ataatggccg cctgatatgt atgcaactat aggagatgtg atgcacgcgc ttcatttttc
     5401 ggcttcggac aaggccgcgc tttatcggga ggtgttgccg cagattgagt ctgtggtggc
     5461 ggacgagacg gattgggtgg cgaatttggc gaacacggcg gcggttttga aggaggcgtt
     5521 cggttggttt tgggtgggtt tttatttggt cgatacgcgt tcggacgaat tggttttggc
     5581 accgtttcag gggcctttgg cgtgtacgcg gattccgttc ggtcgcgggg tgtgcggtca
     5641 ggcttgggcg aagggcggga cggtggtcgt cggggatgtg gacgcgcatc ccgaccatat
     5701 tgcctgttcg tctttgtcgc gttcggagat tgtcgtgccg ctgttttcag acggccgctg
     5761 tatcggcgtg ttggacgcgg acagcgaaca tttggcgcag tttgatgaga cagatgcttt
     5821 gtatttgggc gaactggcga agattttgga gaagcggttt gaggcttcgc gccaggcggc
     5881 ttgagtgatg gaaaggggcg ggcggcgcgt gccgaagttg gcgcggcggg agtgtggttt
     5941 tataatgcct gccattgttg aaacaattat ttgacggagc actaaatgga ttttgaaaaa
     6001 gcgcggttca atatggtcga acagcaaatc cgtccttggg atgtattgga ttttgacgtt
     6061 ttggacgctt tggaggagat tccgcgcgag ctttttgtcg atgaggattt gcagggtttg
     6121 gcgtatgcgg atatggcgct gccgcttgcc aacggtcata agatgctgga gccgaaagtc
     6181 gtggcgcggc tggcgcaggg cttgaagctg acgaaaaacg atacggtttt ggaaatcggc
     6241 acgggttcgg gctatgcgac cgcgcttttg gcaaaattgg ctggtcgtgt ggtgtcggac
     6301 gacatcgatg ccgaacagca aaaccgcgcc aaagcggtgt tggacggctt gtctttggaa
     6361 aacattgatt atgtgcaaaa caacgggttg accgaacttt ccgcaggtgc gccgtttgat
     6421 gcggtttatg tcggcggtgc ggtaaacctt gtgcctgaag tgttgaaaga acagttgaaa
     6481 gatggcggac gcatggtggt tatcgtagga cgcaagcctg ttcaacgcgc gcttttgatt
     6541 acgcgcaggg gcgatgtgtt tgaagagaag gtgctgttcg ataccttggt ggcgcatttg
     6601 gatgataagg atgccgatcc gtttggtggt tttcagtttt gatgttcgga ttgcgatgcc
     6661 gtctgaaagc gggtttgggg cttcagacgg cattttgctt ggtttttttt ggggggtgcg
     6721 atggatattg tgcaacttgg gtcggcggaa ttgaaggcgt ggatggacga agggcggatg
     6781 ttttgtttgt tggacgtgcg tacggatgaa gaaacggcgg tttgttcgct gccaaatgcg
     6841 ctgcatatcc cgatgaatct gattccgctg cggcagaacg agctgccgga tgatgtgccg
     6901 cttgtggtgt attgccacca cggtatccgc agcctgcata cggcgatgta tctggcggac
     6961 gcgggttttg aaaacctgta caaccttcag ggcggcatcg atgcatgggc ggccgagatt
     7021 gacggcaaca tgatgcgtta ttgaggcagg catttcagac ggcattcctt aaatgcgtgt
     7081 acctgtggtg ttccaaaagg tcgtctgaaa gtgcagcttc tgcgaagcta aagcgcggta
     7141 tcaacgaagt taaaatttgc ctgaacctta aaggcagcct gcaccccaat cccctctccc
     7201 gtgggagagg gctaggacga ggctggcaaa ctgaaagttt gcttgggcgg cattttcaat
     7261 gtgcaggctg
//
LOCUS       DAWHXZ010000092         7221 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000092 DAWHXZ010000000
VERSION     DAWHXZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.092"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(71..430)
                     /gene="rnpB"
                     /locus_tag="WIE35_001221"
     ncRNA           complement(71..430)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WIE35_001221"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(509..1612)
                     /gene="prfB"
                     /locus_tag="WIE35_001222"
     CDS             complement(509..1612)
                     /gene="prfB"
                     /locus_tag="WIE35_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ4154135.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVVGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1715..2608)
                     /locus_tag="WIE35_001223"
     CDS             complement(1715..2608)
                     /locus_tag="WIE35_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ4154136.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2950..3399
                     /locus_tag="WIE35_001224"
     CDS             2950..3399
                     /locus_tag="WIE35_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ4154137.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3473..3781
                     /locus_tag="WIE35_001225"
     CDS             3473..3781
                     /locus_tag="WIE35_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154138.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3897..4739
                     /locus_tag="WIE35_001226"
     CDS             3897..4739
                     /locus_tag="WIE35_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ4154139.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4729..5475
                     /locus_tag="WIE35_001227"
     CDS             4729..5475
                     /locus_tag="WIE35_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ4154140.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLST"
     gene            5497..6084
                     /locus_tag="WIE35_001228"
     CDS             5497..6084
                     /locus_tag="WIE35_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ4154141.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6090..6269
                     /locus_tag="WIE35_001229"
     CDS             6090..6269
                     /locus_tag="WIE35_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ4154142.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            6501..6941
                     /locus_tag="WIE35_001230"
     CDS             6501..6941
                     /locus_tag="WIE35_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154143.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1844 a         1926 c         1633 g         1818 t
ORIGIN      
        1 aaacacaacc taaataaaaa tgccgtctga accatatttc aggtttcaga cggcatttgc
       61 gtgtcggatg cacaccggac gggcggtaag ccgggttctg tctcggacag tcattcctct
      121 aggcataccg ttaccggtat gctcaagcaa cctacccgaa cgctcggcgg gcagcgtcat
      181 tgcgttctgt ttggtcttgc tccgaatggg gtttggcctg ccgcatattg ttaccaaatg
      241 cgcggtgcgc ccttaccgca ccttttcacc cttgcctgtg ctgccaaagc agccatcggc
      301 ggttttgctt tctgttccac tttccgtcgc gttaccgcgc ccggccgtta accggcattc
      361 taccctgcgg agcccggact ttcctccccg tatgccttac gcgatacgcg gcgactgtct
      421 gcccgtcccg tgtgcggcgc ggattataac acgaaacgca aaaatgccgt ctgaaacggt
      481 acaggtttca gacggcatac agcctaaact acacgccctg tttcaggctg gcttcgataa
      541 agccgtccaa atcgccatcc aatacggctt tggtgttgcc gacttcgtag cctgtacgca
      601 agtctttgat acgtgaggaa tccaaaacat acgaacggat ttggctgccc caacctacat
      661 cggatttacc ttcttccaac gcctgtttct cttcattgcg tttgcgcatt tccaattcat
      721 acagtttgga cttcaacatt tccatcgcgg cggctttgtt ggcgtgttgc gaacggtcgt
      781 tttgacattg caccacaatc cccgtcggct cgtgggtaat gcgcacggcg gagtcggttt
      841 tattgatgtg ctgaccgccc gcacccgatg cgcgataggt gtcgatgcgc aaatcggcgg
      901 ggttgatttc gatttcgatg gaatcgtcga tttcgggata gacgaacacg gaggcaaacg
      961 aggtatggcg tttgttgttc gagtcaaacg gagagtaacg caccaagcgg tgaacacctg
     1021 tttccgtgcg cagcaagccg taggcatatt cgccttccac tcggatagtc gcgcggttga
     1081 tgcccgcgat ttcgccgtcg tcttcttcga ggatttcgat tttgaagcct ttgcgctcgg
     1141 cgtagcggct gtacatgcgg aacagcatac ccgcccagtc ttccgcttcc gtaccgcctg
     1201 cgcctgcggt aatgtcgata aagcagttgt tcggatcggc gggctggttg aacatccgtt
     1261 tgaactccaa atccgccatc tgtttttcca gccccgccac atcttcctgc acggcggcaa
     1321 aaccttcttc gtcgttttct tcgacggtca tttcaatcag catgcggttg tcttcgatgc
     1381 ccgaagcgat gttgtcgagc gtcaacacga tgccttcgag gattttgcgc tctttgccga
     1441 tttcttgggc gcgtttcggg tcgttccaaa gttcggggtc ttcggaaaga ccgacaactt
     1501 cttccaatcg gtctttctta ccctgataat ccatataaac tcggatgtct tcgctgcgct
     1561 tttccaaatc attcagggta ttgttgagct ggttgattac ttcggcttcc atgattcttt
     1621 tgttctttca aaattttagg ggcgtattgt acgggattcg ggtatttttt tctatggata
     1681 aagccttctg gaaacacgtt cggacggcat agcgtcaata acggtatgcc gccagtttgc
     1741 gtttgatttc aggcaatgcg gcacgtgctg cctcctcacc caaccggatg gcgcgttttt
     1801 tctgatcgaa tccgccgact gcacccaaat ccaaaacctg cggtttgata accacatccg
     1861 cctgccccaa ctcattttgc aacgcggaaa cgctcattac gttcagcgtc tgatcgagat
     1921 aagagaagaa gccttggctg atgtttttgc ccggacgggc ggaaatatcg acggcaatca
     1981 cgaaattcgc cccctgccgc cgggcggcac tgacgggcac gggctgcgac agaccgccgt
     2041 caacatatgt atgcctgccg atgataacgg gttggaacac attgggaatg gcggcggaag
     2101 cgcgcacagc ctgcccggca ttcccttgat tgaaagcgac ggccttgccg gtttcaaaat
     2161 cagtagcaac agcggcaaat ttgatgggaa actgctgaat ctgcctgccg ccgacttttc
     2221 ggttgatgta attttgcagc ttttcgcctt tgataaaacc actggtggac aaggttaaat
     2281 cgactaaatc ggttttgcct aaaatttcgg cttccaattc gaggcggtcg ggcgacatac
     2341 ccgatgcaaa aaggctgccg actatcgaac ctgccgatgt gccggtaacc accttcacag
     2401 gaataccgtt ttctttcaaa accttaataa tacctacatg ggcaaatcct ttagatgcgc
     2461 cgccaccgag tgccaaaccg accactgcgg cgggtttggc ggtttgcacc ggcttgcggg
     2521 cagcattatt tcccgccgtg ccgcaggcgg caagcaacgc ggcggcggcg attgccaaaa
     2581 gcggtctgat ttttgaaaac gttaccatat tttccattcc tttatatatc gcaccccgtc
     2641 aaaaagaggg attgcttttc ttaacaaccc cctttgacag ccaagcaaat gggggctttg
     2701 ttaagtcatc atcaaaatta atatttcttt ttttccttta cggaaattat atttgaaggc
     2761 atactatcca aggcgggaat tatctcacaa caccgccgtt atccaaatat cccgcctttt
     2821 tcctttcctt tccatcaaaa tactttcttt ttatattcat taacttgtta aatcattggc
     2881 tgccgggtgt cagtttttcc gacaaaatcc gtctaatggg gtatcaacag aaccaaaaca
     2941 ggaacactta tgaaaatcgg aacaacttgg cagacggcat ccgctatgct ggttttgcgt
     3001 ctgtttgccg catatgaatt tttggaatcg ggtttgcaaa aatggaacgg ggagaattgg
     3061 ttttccgaaa tcaacgatca gtttccattc ccgttcaact tgctgccgga cgcgttaaac
     3121 tggaatctcg ccatgtatgc ggagctttta ctgcccgtac tgttgctttt gggtttggca
     3181 acgcgtctgt cggcattagg gctgatggtc gttaccgccg tcgcttgggc tgcggttcac
     3241 gccggttcgg gttacaatgt ctgcgacaac ggttataaaa tggctttaat ttatatcgtg
     3301 gtattaatcc cgctgctttt ccagggtgcg ggcggatggt cgctggatac gctgctgaaa
     3361 aaacggtttt gcccccaatg ccgtctgaag caagattgat tcagtcgtgg aatctgactt
     3421 taaacattcc aaccttatct cgttaacttg atattctgaa aaggaaatag aaatgaacaa
     3481 aaacattgct gccgcactcg ccggtgcttt atccctgtct ttggccgccg gtgcagttgc
     3541 tgccaacaaa ccggcaagca acgcaacagg cgttcataaa tccgcccatg gctcttgcgg
     3601 cgcgtccaaa tctgccgaag gttcgtgcgg cgcggctggt tctaaagcag gcgaaggcaa
     3661 atgcggcgag ggcaaatgcg gtgcgaccgt aaaaaaaacc cacaaacaca ccaaagcatc
     3721 taaagccaag gccaaatctg ccgaaggcaa atgcggcgaa ggcaaatgcg gttctaaata
     3781 atcccccccc ttcaaaccaa gccgcgtttt tcagtaaaat gcggcttttt taacggcaaa
     3841 caaagatttt ttaacaagca catcattctt ttgtgccatc cgaaccgggt aaaaatatga
     3901 ttcaacacgc aggcttgggc taccgccgcg acttggcgga agactttctc tcgctttccg
     3961 aaaacagccc gatatgcttt atcgaagccg caccggaaaa ctggctgaaa atgggcggct
     4021 gggcgcgcaa acagtttgac cgtgtggcgg aacggctgcc gctggcgttg cacggattgt
     4081 ctatgtcgct gggcgggcaa gcaccgctgg atactgattt gatagacggc atcaaagaaa
     4141 tgatgcgccg ttacgattgc acgtttttct ccgaccattt gagctactgc cacgacggcg
     4201 gtcatcttta cgatttgttg ccgctgccct tcaccgagga aatggtgcat catacggcgc
     4261 ggcgtatccg tgaagtgcaa gaccgtttgg gctgccgcat cgccgtggaa aacacgtcct
     4321 actatctgca ttccccgctt gccgagatga acgaggtcga gttcctcaac gccgtcgcac
     4381 gtgaggccga ttgcggcatt catctggatg tgaacaatat ctacgtcaac gccgtcaatc
     4441 acggcctgtt gtcgccggag gcttttttga aaaacgttga cgcagatcgc gtgtgctata
     4501 tccatattgc cggacatgac gtggaaacgc cggaattgtt gattgataca catggcgcgg
     4561 cagttttgcc gactgtttgg gacttgctcg aacttgccta tgccaagctg ccgacgattc
     4621 cgcccaccct gttggaacgc gattttaatt tcccgccttt tgccgaactc gaagccgaag
     4681 tcgccaaaat cgccgattat caaacgcgtg ccggaaagga ataccgccgt gcagcctgaa
     4741 acctccgccc aataccagca ccgtttcgcc caagccatac gcgggggcga agccgcagac
     4801 ggtctgccgc aagaccgact gaacgtctat atccgcctga tacgcaacaa tatccacagc
     4861 tttatcgacc gctgctatac cgaaacgcgg caatactttg acagcgaaga atggagccgt
     4921 ctgaaagaag gtttcgtccg cgacgcgcac gcccaaacgc cctattttca agaaatcccc
     4981 ggcgagttcc tacaatattg ccaaagcctg ccgctttcag acggcatttt ggcactgatg
     5041 gattttgaat atacccaatt gctggcagaa gttgctcaaa ttccggatat tcccgacatt
     5101 cattattcaa atgacagcaa atacacaccc tcccctgcgg cctttatccg gcaatatcga
     5161 tatgatgtta ccgatgattt gcatgaagcg gaaacagcct tgttaatatg gcgaaacgcc
     5221 gaagatgatg tgatgtacca aacattggac ggcttcgata tgatgctgct agaaataatg
     5281 ggttcctccg cgctttcgtt tgacaccctc gcccaaaccc ttgtcgaatt tatgcctgag
     5341 gacgataatt ggaaaaatat tttgcttggg aaatggtcag gctggactga acaaaggatt
     5401 atcatcccct ccttgtccgc catatccgaa aatatggaag gcaattcccc gggccaaaac
     5461 catctatcca cataaaatta ccttgttccc gatactatgc cgctacccga cctgaccgat
     5521 gccgaattaa tagagtcgcg taaactgctt ctgcattttg cgcggcttca gttgcccgac
     5581 caccctgatt tggctgaaga tttagtgcag gaaacattgc tgtccgcata cagcgcaggc
     5641 gacagttttc aaggcagggc acttgtcaac agctggcttt ttgccatatt gaaaaacaaa
     5701 attattgacg cattacgtca aatcggaagg cagaggaaag tctttaccac actggatgac
     5761 gagctactgg atgaagcatt tgaaagccat ttttcccaaa acgggcattg gacgccggaa
     5821 gggcagccgc aacattggaa cactccggaa aaatcattaa acaacaacga attccaaaaa
     5881 attctgcaaa gctgcctata caacttgcct gaaaacaccg cacgggtatt taccctgaag
     5941 gaaatactcg gtttttcatc cgacgaaata caacaaatgt gcggtatcag cacgtccaac
     6001 taccacacca ttatgcaccg cgcccgagaa tcattgcgcc aatgcctgca aatcaaatgg
     6061 ttcaaccaag aaaacccgaa gtaaacgtta tgaaaaaatg ccgcgatatc gccctgcttc
     6121 tttccaaaca tcaggaccgg gaaaccaccc cgggcgagaa gatttccata tacacacacc
     6181 tgctgttctg cccacattgc cgagaatata aaagacagct tcaaaccatc aaaatatcac
     6241 tggcaaaaac aaccagaact tcaaaataaa tgccgtctga aaaggcttca gacggcataa
     6301 gctgacggaa aaaaatcaaa ccgatttact gttatcggca gttcatccat aatacacgct
     6361 tcaaaagcag catattcctc catacggaat atataaatac gcaaaacacg aaggctgcat
     6421 caatttgctt tatttgcctt atttcacaga cggcgctacc cctcccgccc aacccgttct
     6481 ttctgaatga gcagatttca atgattaagg aaaccctaat gcgcccaatc ttcctatctt
     6541 tcgttttact ccctattttg ataaccgcct gcagcacacc ggacaagtct gcccgatggg
     6601 aaaatatcgg cacaatctca aacggcaata ttcatacata tatcaataaa gacagcgtga
     6661 gaaaaaacgg aaatctgatg attttccaag ataaaaaagt tgttaccaat ctaaaacaag
     6721 aacgttttgc caacaccccc gcatacaaga ctgccattgc cgagtgggaa atccactgca
     6781 acaacaaaac ataccgctta agttcgctac agttgtttga tacaaaaaac acggaaattt
     6841 ccacacaaaa ctacacagcc tcttccctcc gcccgatgag catcctgtcc gggacattaa
     6901 ccgaaaaaca atatgaaacc gtatgcggaa aaaaactctg attgcaactt atacacaaac
     6961 ttacccacaa accttatcat aaaaatgccg tctgaaatac tgaaatatca gcatttcgga
     7021 cggcattttg ccatttctcc gaaaagttat cctcgaagtt atccacatta tttttttaaa
     7081 ccagcttcca tccgaaatat aaacctgcta tacaatttca ttatcaaaac gtaaattgtt
     7141 ccattgatac acaaaactgc ttacccccat aattttgata aagcatttct tacattcccg
     7201 gctccgtccc gtaatcaaca c
//
LOCUS       DAWHXZ010000093         7156 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000093 DAWHXZ010000000
VERSION     DAWHXZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.093"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..561
                     /locus_tag="WIE35_001231"
     CDS             <1..561
                     /locus_tag="WIE35_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HEZ4154144.1"
                     /translation="RVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQFGKI
                     GYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVEKFPA
                     VHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPSLVLF
                     LFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            562..1368
                     /locus_tag="WIE35_001232"
     CDS             562..1368
                     /locus_tag="WIE35_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ4154145.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFAMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(1484..1639)
                     /locus_tag="WIE35_001233"
     CDS             complement(1484..1639)
                     /locus_tag="WIE35_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154146.1"
                     /translation="MYTGHYGGNTVWEAAASRGIGTAGDAPMPSGVKAGQSAVWKKAM
                     ESVGRRS"
     gene            complement(2335..2787)
                     /locus_tag="WIE35_001234"
     CDS             complement(2335..2787)
                     /locus_tag="WIE35_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ4154147.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            complement(2804..3034)
                     /locus_tag="WIE35_001235"
     CDS             complement(2804..3034)
                     /locus_tag="WIE35_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ4154148.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            complement(3041..3343)
                     /gene="priB"
                     /locus_tag="WIE35_001236"
     CDS             complement(3041..3343)
                     /gene="priB"
                     /locus_tag="WIE35_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ4154149.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHKSWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            complement(3344..3712)
                     /gene="rpsF"
                     /locus_tag="WIE35_001237"
     CDS             complement(3344..3712)
                     /gene="rpsF"
                     /locus_tag="WIE35_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ4154150.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            complement(3868..4818)
                     /gene="trxB"
                     /locus_tag="WIE35_001238"
     CDS             complement(3868..4818)
                     /gene="trxB"
                     /locus_tag="WIE35_001238"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ4154151.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            4957..7119
                     /locus_tag="WIE35_001239"
     CDS             4957..7119
                     /locus_tag="WIE35_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ4154152.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAYGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMYAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDEDALYRI
                     AAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKAGKAEF
                     AELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLRNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
BASE COUNT         1677 a         1901 c         1779 g         1799 t
ORIGIN      
        1 cgcgtggcgc ggctgaccaa cagccaaagc gcgttcgggg agcagctcga cagccttgcc
       61 gatatggtca gcttcggcgt tgctcccgct ctgattgcct acaaatggca gctttggcag
      121 ttcggcaaaa tcggttattc cgtcgccttc atctactgcg cctgcgccgc cctgcgcctc
      181 gccctgttca acacactcat cggcaaggtg gacaaacgct ggtttatcgg cgtgcccagt
      241 ccgactgccg ccgcgctgat tgtcgggctg atttgggtca accacagcgt cgaaaaattc
      301 cccgccgtcc actggtgggc attgggcatc acactgtttg ccggcctgtc gatgattgtc
      361 caaatccctt tttggagttt taaagaaatc aacatccgca gacaagtccc ctttgtcgga
      421 atgctgcttg ccgtcttatt gctgcttctg gtcacttggg aaccgtcgct cgtcctcttc
      481 ctgttctttc tcggatacag cctgtccggc tacattatgg cggcacgccg attctggaaa
      541 aaatacagaa aggcggatta aatgtggcat tgggacatta tcttaatcct gcttgccgta
      601 ggcagtgcgg caggttttat tgccggtctg ttcggcgtag gcggcggcac gctgattgtc
      661 cctgtcgttt tatgggtact tgatttgcag ggtttggcac aacatcctta cgcgcaacac
      721 ctcgccgtcg gcacatcctt cgccgtcatg gtcttcaccg ccttttccag tatgttgggg
      781 cagcacaaaa aacaggcggt cgactggaaa accgtatttg cgatgatgcc gggtatgata
      841 ttcggcgtat tcacgggcgc actctccgca aaatatatcc ccgcgttcgg gcttcaaatt
      901 ttcttcatcc tgtttttaac cgccgtcgca ttcaaaacac tgcataccga ccctcagacg
      961 gcatcccgcc cgctgcccgg actgcccgga ctgactgcgg tttccacact gttcggcaca
     1021 atgtcgagct gggtcggcat aggcggcggt tcactttccg tccccttctt aatccactgc
     1081 ggcttccccg cccataaagc catcggcaca tcatccggcc ttgcctggcc gattgcactc
     1141 tccggcgcaa tatcgtatct gctcaacggc ctgaatattg caggattgcc cgaagggtca
     1201 ctgggcttcc tttacctgcc cgccgtcgcc gtcctcagcg cggcaaccat tgcctttgcc
     1261 ccgctcggtg tcaaaaccgc ccacaaactt tcttctgcca aactcaaaaa atccttcggc
     1321 attatgttgc ttttgattgc cggaaaaatg ctgtacaacc tgctttaaaa cacacgaaaa
     1381 aaccttttta ccgtttgcac aagcaattaa tcaggacaaa gctgcccagt ctcctgttcc
     1441 gacaaaagga cagacaacct gaccgtgcgc ccgactttta cccttaactt cttctgccca
     1501 cagattccat tgcctttttc caaactgccg attgccccgc cttcacgccc gaaggcatcg
     1561 gcgcatcgcc tgccgtgccg attccacgcg atgcggctgc ctcccaaacg gtatttccgc
     1621 cataatgacc ggtatacaag ccgcaacctt tgcaatcata tatataagtt attaaatttt
     1681 taggatgtat tttgacaatc tgttgatttc aactgcttgc aagggaaaag gccattattt
     1741 ttcggtcaat caggaagtta tcctattgaa cattttgaaa caaagtatgt tatcagcacc
     1801 atattgaaac acagccaaat tttatgcccg ataccatttt caaaataaat aatcccctga
     1861 ttttcacaga tttctcaaaa tcagaaaagt ttttgtcaat tgaatatttt ggctgtgttt
     1921 tagtaatctg ttgatttcaa ttatttgcaa gggaaaagac aattattttt tcggttagga
     1981 agagatctat cctattgagt atattgaagc caggtacgct tatcaacact atattaaaac
     2041 acagccaata ttttttggcg cattttatgc atcaaatttc gttaacaaac tattcctgca
     2101 aagaccacag gcaaatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
     2161 caatacagat agcacggcaa ggcaaggcga ggcaacgctg tactggttta aatttaatcc
     2221 actataaaaa caaccatccc gatttttgca gaatatacgg aaaacaaaac aaataccgtc
     2281 tgaaaccaca ttccgacaat cggcagggtt tcagacggca tctgataatt tcaattactc
     2341 ggttgcggca acgacggcaa cggtaatttt agcaacggca tcagtgtgca aagccacttc
     2401 cacttcgtac tcgccaacgg ctttcagagg accgttcggc agacgtacat ttgctttcac
     2461 ggcttcgatg ccggcagcaa cgattgcagc agcaatgtcg gcattggtaa cggaaccgaa
     2521 caggcggccg tccacaccgg ctttttgagc aacggtaacg gtttgaccgt ccaatttttc
     2581 ctgacggact cgggcatctg ccaaaatttc agcctgtttg gcttccaatt cggcacggcg
     2641 tgcttcaaac tctttcatat tcgcttcggt cgcacgtttt gccttacctg cgggaattag
     2701 aaagttgcgg gcgtagccgt ttttaacggt tacgatgtcg cccaagttgc ccagaccgcc
     2761 gattttttct aacagaataa tttgcatgat tcaatctcca aaattatttg tgttggtcgg
     2821 tgtaaggcag cagagccagg aagcgtgcgc gttttacggc aacagccaat tggcgttggt
     2881 agaatgcctt cgttcctgtg atgcgtgcag gaatgatttt accgttttca gagataaagt
     2941 ctttcagcaa atcaacttgt ttgtaatcga cttcttggat tttttcagcc gtgaaacggc
     3001 agaattttct acgtttgaat gattgacgag ccattgtcgt ttaaccttta tattcttgaa
     3061 tattttgtat cctgagcatc ggcataaggg aacgtctgct tttttgagct aaaaaacctt
     3121 cgacgtgaac atatacacct tgccgatact gccactcttc cgcctgcctg cctaaaatcc
     3181 gtgccggaat ttccaattgg acaaggcatt gctgcccgtt ttcctcctgc cacgatttgt
     3241 gctttaaaat aatatctaaa acagggattc cggcaggcgt atatcgaata gggaaaacct
     3301 tttcaattaa cgcggcaagc gaaacaagat tattgaatcc caattattgg gcgaccgctt
     3361 cttcagacgc accgctcaac aggttcttag ccttttcacc acccaacata ggggatgctt
     3421 cggtaacggc gtgtttggtt ttgatggtta gatggcgcaa tacggcatca ttgaagcgga
     3481 atgcggtttc cagctcttca accacttcgg gagtggtttc gatgttcatc aaaacgtaat
     3541 gggctttatg gattttgtta atcgggtaag ccagttggcg gcgaccccaa tcttccaaac
     3601 ggtgaatctt accgtttgct tcggcaatca tggttttgta acgttcaacc atagcaggca
     3661 cttgctcgct ttgatcagga tgaacgataa acacgatctc gtaatgacgc atgttatctc
     3721 cttatggatg gtaaaaacag ccttctgcca tgcgaaagca gaaagcaagg ttcaaagagc
     3781 aggcattata ttggggtttg ccgacggaat caaggatttg gtgcgaaaaa tttgcattcc
     3841 gccgaaaatt tcggtttcag acggcattca aatgttttgg ctgcccagcc agcgttccgc
     3901 gtccaaagcc gcctgacagc cggaagccgc gctggtaatc gcctgacggt aggtatggtc
     3961 ttttacgtcg cccgccgccc atacgccttc gatattggtt gcgccgacat tgtccgccgt
     4021 gccgcctttg gttttcaggt aaccggcttc gtccatttcc aactgacctt tgaaaatatc
     4081 ggtattcggc ttgtgcccga tggcgataaa aatgccgctg acggcaattt gttgctcaga
     4141 accgtcgttg ttttttaata atgcgccgtt tacgccccga tcgtcgccca gtacttcttg
     4201 caggttgctt tccagcttga ggatgatttt gccctcttcc acgcgtttca tcagtttgtc
     4261 gatcatgatt ttttcggcac ggaactcgct gcggcggtgg atcagggtaa cggttttggc
     4321 gatattggca aggtagagtg cctcttcaac tgccgtattg ccgccgccaa ctacggcaac
     4381 atcttggttt ttgtagaaga aaccgtcgca ggtggcacaa gcggaaacgc cttttcctgc
     4441 aaacgcttcc tcactcggca aaccgaggta tttggcggac gcgcctgttg cgacaatcag
     4501 ggcatcgcaa gtgtactcgc ccatatcgcc tttgagtgta aacgggcgtt tttgcagatc
     4561 gacggcgttg atttggtcaa aaatgatttc cgttccgaaa cgttcggcgt gggcgagaaa
     4621 ccgcgccatc aattccggcc cttgcacgcc gtcggcatcg gcaggccagt tgtccacttc
     4681 ggtggtggtc atcagttgcc cgccttgcgc gatacctgta ataatgacgg ggtttaaatt
     4741 ggcacgcgcg gcatagacgg cggcggtgta tccggcgggg ccggaaccca aaataatcag
     4801 tttgcggtgt tgggacattg tttttccttt gctgtgtcaa gttttcggat tctactcgaa
     4861 ttatcggcgc gtttgagaaa tttcgaccat accggcgctc agacggcatc ccgcagcctt
     4921 aactgccgtc tgaatatcaa agcaggaatc acgcttatgc aacaaaaaat ccgtttccaa
     4981 atcgaaggca tgacctgcca ggcctgcgct tcgcgcattg aaaaagtgtt gaacaaaaaa
     5041 gattttgtcg aatcggcggg ggtaaacttc gccagcgaag aggcgcaggt agtgtttgac
     5101 gacagcaaaa cctcagtagc cgacattgcc aaaatcattg agaaaaccgg ttacggcgcg
     5161 aaggaaaaaa cggaagatac attgccgcaa cccgaagcag aacaccatat cggctggcgg
     5221 ctgtggctgc tgttcaccat caacgtcccg ttccttatcg gcatggcggg gatgatgatc
     5281 ggcagacacg attggatgat tccgccgttg tggcagttcg cattggcaag cgtggtgcag
     5341 ctttggctgg caatcccgtt ttacaaaagc gcgtgggcga gcattaaggg cggactggcg
     5401 aatatggacg tgctggttac catcggcacg gtctcgattt acctgtattc cgtctatatg
     5461 ctgtttttca gcccgcacgc ggcgtacggt atggcgcatg tgtattttga agtgggcgtg
     5521 atggtgatcg gttttgtgtc actgggtaaa tttttggaac accgtaccaa aaaatccagc
     5581 ctcaacagct tgggcttgct gctcaaactt acaccaaccc aagtcaacgt gcaacgcaac
     5641 ggcgaatgga aacagcttcc catcgaccaa gtgcaaatcg gcgaccttat ccgcgccaac
     5701 cacggcgaac gcattgccgc agacggcatc attgaaagcg gcagcggttg ggcggacgag
     5761 agccatctta ccggcgaatc caatcctgaa gaaaaaaagg cgggcggcaa agtgttggcg
     5821 ggcgcgttaa tgaccgaagg cagtgtggtg taccgcgcca cgcagctcgg cagccaaacc
     5881 cagctcggcg acatgatgaa cgcgctctct gaagcacaag gcagtaaagc accgattgcg
     5941 cgcgtagccg ataaagcggc tgcggtattc gtgcctgccg tcgtgggcat tgcgttgttg
     6001 acttttattg ttacttggct gattaagggc gattggacgg ttgcgctgat gtacgccgtc
     6061 gccgttttgg tgattgcctg cccgtgcgcg ctgggtctgg caacccctgc cgcgattatg
     6121 gtcggtatgg gcaaagcggt taaacacggt atttggttta aagacgcggc agcaatggag
     6181 gaagccgccc acgtcgatgc cgtcgtgttg gacaaaaccg gtacgctgac cgaaggcagc
     6241 ccgcaggttg ccgccgttta ttgcgttccc gacagcggct ttgacgaaga cgctttgtac
     6301 cgcatcgccg ccgccgtcga acaaaacgcc gcccatccgc tcgcccgtgc catcgtctcc
     6361 gccgcccaag cgcgcggttt ggacattccc gccgcacaaa acgcacaaac cgttgtcggc
     6421 gcaggcatta ccgccgaagt ggaaggcgtg ggtttggtga aagcaggcaa agccgaattt
     6481 gccgaactgg ccttgccgaa gtttttagac ggcgtttggg atattgcaag cattgttgcg
     6541 gtctcagtcg ataacaaacc catcggcgca ttcgcacttg ccgacgcgtt gaaagccgat
     6601 accgccgaag ccataggccg tctgaaaaaa cacaatatcg atgtctatat tatgagcggc
     6661 gacaaccaag gcacggtcga atacgtcgcc aaacaactgg gcatcgcaca cgccttcggt
     6721 aatatgagtc cgcgcgacaa agccgccgaa gtgcagaaac tcaaagccgc cggcaaaacc
     6781 gtggcgatgg tcggagacgg catcaacgac gcacctgcgc tcgccgccgc caacgttagc
     6841 ttcgccatga aaggcggcgc ggacgttgcc gaacacaccg catccgccac gctgatgcaa
     6901 cattcggtca accaactcgc cgatgcccta tcggtatcgc gggcgacgtt gagaaacatc
     6961 aagcaaaacc tgtttttcgc cttcttctac aatattttgg gcattccgct tgccgcgctc
     7021 ggttttttaa accctgtcat cgcaggcgcg gcaatggcgg caagctcggt ttccgtgttg
     7081 agcaacgcct tgcgcctgaa acgggtaaaa atcgattgac cgcatataac cacccgacag
     7141 cttttccgaa cggata
//
LOCUS       DAWHXZ010000094         7148 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000094 DAWHXZ010000000
VERSION     DAWHXZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.094"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(80..187)
                     /locus_tag="WIE35_001240"
                     /pseudo
     CDS             complement(80..187)
                     /locus_tag="WIE35_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            197..499
                     /locus_tag="WIE35_001241"
     CDS             197..499
                     /locus_tag="WIE35_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154153.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            783..1031
                     /locus_tag="WIE35_001242"
     CDS             783..1031
                     /locus_tag="WIE35_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ4154154.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTDL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGDFGEFCKGLIL"
     gene            1092..2834
                     /gene="hrpB"
                     /locus_tag="WIE35_001243"
     CDS             1092..2834
                     /gene="hrpB"
                     /locus_tag="WIE35_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ4154155.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETASGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQF
                     SYETAIHAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLSGERGFYWQNTLGWHFHPRH
                     QFYLGADYGRVSGESAQYASGKRLAGAVAGFRGSHKVGGIFAYDLFAGKPLHKPEGFQ
                     TANTVYGFNLNYSF"
     gene            2927..>7148
                     /gene="hrpA"
                     /locus_tag="WIE35_001244"
     CDS             2927..>7148
                     /gene="hrpA"
                     /locus_tag="WIE35_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HEZ4154156.1"
                     /translation="MNKGLHRTVFSKKHNTMVAVAETANSQGKGKQAGTSAPASPQIS
                     DGLYSKLKTTLKTLVCSLVSVSMVLPAHAQIITDKTAPKNQQAVILKTNTGAPLVNIQ
                     TPNARGLSHNRYTQFDVDAKGAVLNNDRNNNPFLAKGSAQLILNEVRGAASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGTPQIGKDGALTGFDVRQGTLTVGT
                     SGWNDKGGADYTEVLARAVALQGKLQGKNLAVSTGAQKVDYASGEISAGTAAGTKPTV
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADS
                     TEASPTYLSIETTETGAAGAFISNGGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSTGGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARADAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLSAPGGYIVDIPKGNLKTEIEK
                     LSKQPEYAYLKQLQVAKNINWNQVQLAYD"
BASE COUNT         2096 a         1759 c         1651 g         1642 t
ORIGIN      
        1 tttgtaaaag caaggatagg gcatctgccc ttttgattgt ttgattaacg atacaaggag
       61 tttcaaaatg agagttttat agtggattaa atttaaacca gtacagcgtt gcctcgcctt
      121 gccgtaccat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttaaa tttaatccac
      181 tatatgtgtt catgaaatga cttgggtcgg aggctcaggt aatgcgcaac aaagttcata
      241 ttattgcgaa atttgcgaat ctgcagggct taacgatacg ggaaatcctg ataaatcttt
      301 aggattgcca aacaatacgt tcagtaatcc gcctggttgg ggagctacaa tcggagcttt
      361 agcaggtagc cgcataggga tgcctgaatt tggtacgttt gcgagccatg ccattgaaaa
      421 tttcgactgg tcatggtatc gacgttatag ggaaattgcc gaaacgattg aacgagaata
      481 ttcaggcggt ttgccttaat agttgaggag gtcatgatgt ttgccaaaca ttatcaattc
      541 atcgcactcg gcatcatgct gcttctttat atgttgattc tctatacgac cgatttttcc
      601 aatctgacgt attggatgct gttttttatc tgttttatta caggaaaaat attagctcgt
      661 ttgttagaga aaagctttaa ataaaatagc agctagtcgc aaaaggtcgt ctgaaacctt
      721 ttcaggcggc ctttctaaaa tacatccaac ttcctaatcc ctatttttca aaaaggaaat
      781 ctatgcccca tctgcaaaac ctgtctttgg gcttaaagaa aaagctgcct gttatcctgc
      841 aaacagaaat atcagaatgc ggcttggcat gtctggcggc tgtggcggga tttcatggtt
      901 tccatacgga tttacgcgca ctgcgttcaa aatactgtcc gagacctttg caaaattccc
      961 caaaatcccc taaatgtctt ggtgggaatt ttggggattt tggggaattt tgcaaaggtc
     1021 tcattctata actgtaaata cttttaaatt tatgacagaa tagtaaatat tgctaaaata
     1081 atattaatgt catgaaattt tttcctgctc catgtctgtt ggttatcctg gctgtcatac
     1141 cccttaaaac cttagctgcc gatgaaaacg atgcagaact tatccgttcc atgcagcgtc
     1201 agcagcacat agatgctgaa ttgttaactg atgcaaatgt ccgtttcgag caaccattgg
     1261 agaagaacaa ttatgtcctg agtgaagatg aaacaccgtg tactcgggta aattacatta
     1321 gtttagatga taagacggcg cgcaaatttt cttttcttcc ttctgtgctc atgaaagaaa
     1381 cagcttttaa aactgggatg tgtttaggtt ccaataattt gagcaggcta caaaaagccg
     1441 cgcaacagat actgattgtg cgtggctacc tcacttccca agctattatc caaccacaga
     1501 atatggattc gggaattctg aaattacggg tatcagcagg cgaaataggg gatatccgct
     1561 atgaagaaaa acgggatggg aagtctgccg agggcagtat tagtgcattc aataacaaat
     1621 ttcccttata taggaacaaa attctcaatc ttcgcgatgt agagcagggc ttggaaaacc
     1681 tgcgtcgttt gtcgagtgtt aaaacagata ttcagattat accgtccgaa gaagaaggca
     1741 aaagcgattt acagatcaaa tggcagcaga ataaacccat acggttcagt atcggtatag
     1801 atgatgcggg cggcaaaacg accggcaaat atcaaggaaa tgtcgcttta tcgttcgata
     1861 accctttggg cttaagcgat ttgttttatg tttcatatgg acgcggtttg gcgcacaaaa
     1921 cggacttgac tgatgccacc ggtacggaaa ctgcaagcgg atccagaagt tacagcgtgc
     1981 attattcggt gcccgtaaaa aaatggctgt tttcttttaa tcacaatgga catcgttacc
     2041 acgaagcaac cgaaggctat tccgtcaatt acgattacaa cggcaaacaa tatcagagca
     2101 gcctggccgc cgagcgcatg ctttggcgta acaggtttca taaaacttca gtcggaatga
     2161 aattatggac acgccaaacc tataaataca tcgacgatgc cgaaatcgaa gtgcaacgcc
     2221 gccgctctgc aggctgggaa gccgaattgc gccaccgtgc ttacctcaac cgctggcaac
     2281 tggacggcaa gttatcttac aaacggggta ccggtatgcg ccaaagtatg cccgcacctg
     2341 aagaaaacgg cggcggtact attccaggca catcccgtat gaaaatcata accgccggat
     2401 tggatgcagc ggccccgttt atgttgggca aacagcagtt ttcctacgaa accgctatcc
     2461 atgcacaatg gaacaaaacg cctttggttg cccaagacaa gttgtctatc ggcagccgct
     2521 acaccgttcg aggatttgac ggtgaacaga gcctttccgg agagcgtggt ttctactggc
     2581 agaacacttt gggctggcat ttccatccgc gccatcaatt ctatctcggc gcggactatg
     2641 gtcgggtatc gggcgaaagt gcccaatatg cctcgggcaa gcggctggcg ggtgcagtgg
     2701 ccggcttcag aggcagccat aaagtaggcg gtatatttgc ttatgactta tttgccggca
     2761 agccgctgca caagcccgaa ggttttcaga cggccaacac cgtttacggc ttcaatttga
     2821 attacagttt ctaaccctct cctgcatttt ttaccgacat tcagacagtc tttcatcatt
     2881 tacaggccgt ctgaagcgat tacaaacatc atttaaaggg ataaatatga acaaaggtct
     2941 gcatcgtacc gttttcagca aaaagcacaa cactatggtt gcagttgccg aaaccgccaa
     3001 cagtcaggga aaaggcaaac aggcaggaac ttccgctccg gcttcgccac aaatttcaga
     3061 cggcctttac agtaaactca aaaccacgct taaaaccttg gtctgctctt tggtttccgt
     3121 cagtatggta ttgcctgccc acgcacaaat cattaccgat aaaaccgcgc ccaaaaacca
     3181 gcaggccgtt attcttaaaa ccaataccgg tgcgccgttg gtcaacatcc aaactcctaa
     3241 tgcccgtgga ttgagccaca accgctatac ccagtttgat gtcgacgcta aaggtgctgt
     3301 actgaacaac gatcgcaaca acaacccgtt tctggccaaa ggcagtgcgc agttgatttt
     3361 gaacgaggtg cgcggtgcgg ccagcaaact caatggcatc gttaccgtag gtggtcaaaa
     3421 ggccgatgtg attatcgcca accccaacgg cattaccgtg aacggcggcg gctttaaaaa
     3481 cgtcggccgc ggtatcttaa ccaccggtac gcctcaaatc ggcaaagacg gtgccttgac
     3541 gggatttgac gtgcgccaag gcacattgac cgtcggtacg tcaggttgga acgacaaagg
     3601 cggagccgat tacaccgaag tgcttgcccg tgcggttgct ttgcagggga aattgcaggg
     3661 taaaaacctg gcggtttcga ccggcgcaca gaaagtagat tacgccagcg gtgaaatcag
     3721 cgcaggtacg gcagcgggta cgaaaccgac cgttgccctc gatactgccg cattgggtgg
     3781 tatgtacgcc gacagcatta ccctgattgc caatgaaaaa ggcgtgggtg tcaaaaatgc
     3841 gggcacactc gaagcggcca agcaattgat cgttacttcc tccggccgta ttgaaaacag
     3901 cggccgcatc gccaccactg ccgacagcac tgaagcatca ccgacttatc tctccatcga
     3961 aaccaccgaa acaggcgcag caggcgcatt tatctccaat ggcggccgga tagagagcaa
     4021 aggcttattg gttattgata ccggggagga catcggtttg cgtaacggag cggtcgttca
     4081 aaacaacggc aaccgccctg ctgctacggt attaaatgcc ggccgtaatt tggttatcga
     4141 aagcaaagca aatgtgaaca atgctaaagg ttcgaccaat ctgtcgaccg gcggccgtac
     4201 cgtcatcaag gaggccagta ttcagactgg cactaccgta tacagttcca gcaaaggcaa
     4261 cgccgaatta ggcaataaca cacgcattac cggggcagat gttaccgtat tatccaacgg
     4321 caccatcagc agttccgccg taatagatgc caaagacacc gcacacatcg aagcaggcaa
     4381 accgctttct ttggaagctt caacagttac ctccgatatc cgcttaaacg gaggcagtat
     4441 caagggcggc aagcagcttg ctttactggc agacgataac attactgcca aaactaccaa
     4501 tctgaatact cccggcaatc tgtatgttca tacaggtaaa gatctgaatt tgaatgttga
     4561 taaagatttg tctgccgcca gcatccattt gaaatcggat aacgctgccc atattaccgg
     4621 caccagtaaa accctcactg cctcaaaaga catgggtgtg gaggcaggct cgctgaatgt
     4681 taccaatacc aatctgcgta ccaactcggg taatctgcac attcaggcag ccaaaggcaa
     4741 tattcagctt cgcaatacca agctgaacgc agccaaggct ctcgaaacca ccgcattgca
     4801 gggcaatatc gtttcagacg gccttcatgc tgtttctgca gacggtcatg tatccttatt
     4861 ggccaacggt aatgccgact ttaccggtca caataccctg acagccaagg ccgatgtcaa
     4921 tgcaggatcg gttggtaaag gccgtctgaa agcagacaat accaatatca cttcatcttc
     4981 aggagatatt acgttggttg ccggcaacgg tattcagctt ggtgacggaa aacaacgcaa
     5041 ttcaatcaac ggaaaacaca tcagcatcaa aaacaacggt ggtaatgccg acttaaaaaa
     5101 ccttaacgtc catgccaaaa gcggggcatt gaacattcat tccgaccggg cattgagcat
     5161 agaaaatacc aagctggagt ctacccataa tacgcatctt aatgcacaac acgagcgggt
     5221 aacgctcaac caagtagatg cctacgcaca ccgtcatcta agcattaccg gcagccagat
     5281 ttggcaaaac gacaaactgc cttctgccaa caagctggtg gctaacggtg tattggcact
     5341 caatgcgcgc tattcccaaa ttgccgacaa caccacgctg agagcgggtg caatcaacct
     5401 tactgccggt accgccctag tcaagcgcgg caacatcaat tggagtaccg tttcgaccaa
     5461 gactttggaa gataatgccg aattaaaacc attggccgga cggctgaata ttgaagcagg
     5521 tagcggcaca ttaaccatcg aacctgccaa ccgcatcagt gcgcataccg acctgagcat
     5581 caaaacaggc ggaaaattgc tgttgtctgc aaaaggagga aatgcaggtg cgcctagtgc
     5641 tcaagtttcc tcattggaag caaaaggcaa tatccgtctg gttacaggag aaacagattt
     5701 aagaggttct aaaattacag ccggtaaaaa cttggttgtc gccaccacca aaggcaagtt
     5761 gaatatcgaa gccgtaaaca actcattcag caattatttt cctacacaaa aagcggctga
     5821 actcaaccaa aaatccaaag aattggaaca gcagattgcg cagttgaaaa aaagctcgcc
     5881 taaaagcaag ctgattccaa ccctgcaaga agaacgcgac cgtctcgctt tctatattca
     5941 agccatcaac aaggaagtta aaggtaaaaa acccaaaggc aaagaatacc tgcaagccaa
     6001 gctttctgca caaaatattg acttgatttc cgcacaaggc atcgaaatca gcggttccga
     6061 tattaccgct tccaaaaaac tgaaccttca cgccgcaggc gtattgccaa aggcagcaga
     6121 ttcagaggcg gctgctattc tgattgacgg cataaccgac caatatgaaa ttggcaagcc
     6181 cacctacaag agtcactacg acaaagctgc tctgaacaag ccttcacgtt tgaccggacg
     6241 tacgggggta agtattcatg cagctgcggc actcgatgat gcacgtatta ttatcggtgc
     6301 atccgaaatc aaagctccct caggcagcat agacatcaaa gcccatagtg atattgtact
     6361 ggaggctgga caaaacgatg cctatacctt cttaaaaacc aaaggtaaaa gcggcaaaat
     6421 catcagaaaa accaagttta ccagcacccg cgaccacctg attatgccag cccccgtcga
     6481 gctgaccgcc aacggtatca cgcttcaggc aggcggcaac atcgaagcta ataccacccg
     6541 cttcaatgcc cctgcaggta aagttaccct ggttgcgggt gaagagttgc aactgctggc
     6601 agaagaaggc atccacaagc acgagttgga tgtccaaaaa agccgccgct ttatcggcat
     6661 caaggtaggc aagagcaatt acagtaaaaa cgaactgaac gaaaccaaat tgcctgtccg
     6721 cgtcgtcgcc caaactgcag ccacccgttc aggctgggat accgtgctcg aaggtaccga
     6781 attcaaaacc acgctggccg gtgcggacat tcaggcaggt gtaggcgaaa aagcccgtgc
     6841 cgatgcgaaa attatcctca aaggcattgt gaaccgtatc cagtcggaag aaaaattaga
     6901 aaccaactca accgtatggc agaaacaggc cggacgcggc agcactatcg aaacgctaaa
     6961 actgcccagc ttcgaaagcc ctactccgcc caaattgtcc gcacccggcg gctatatcgt
     7021 cgacattccg aaaggcaatc tgaaaaccga aatcgaaaag ctgtccaaac agcccgagta
     7081 tgcctatctg aaacagctcc aagtagcgaa aaacatcaac tggaatcagg tgcagcttgc
     7141 ttacgaca
//
LOCUS       DAWHXZ010000095         7019 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000095 DAWHXZ010000000
VERSION     DAWHXZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.095"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(190..402)
                     /locus_tag="WIE35_001245"
     CDS             complement(190..402)
                     /locus_tag="WIE35_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4154157.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWTEKTNAV"
     gene            complement(414..695)
                     /locus_tag="WIE35_001246"
     CDS             complement(414..695)
                     /locus_tag="WIE35_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154158.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(742..1005)
                     /locus_tag="WIE35_001247"
     CDS             complement(742..1005)
                     /locus_tag="WIE35_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154159.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1025..1597)
                     /locus_tag="WIE35_001248"
     CDS             complement(1025..1597)
                     /locus_tag="WIE35_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEZ4154160.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            1576..1887
                     /locus_tag="WIE35_001249"
     CDS             1576..1887
                     /locus_tag="WIE35_001249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154161.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSGLSSVLPASLRRLRVGLTLTASSLRYSVARLMPNSCACCLRYAERAPRSIAS
                     A"
     gene            complement(1909..2784)
                     /locus_tag="WIE35_001250"
     CDS             complement(1909..2784)
                     /locus_tag="WIE35_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ4154162.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2829..3119)
                     /locus_tag="WIE35_001251"
     CDS             complement(2829..3119)
                     /locus_tag="WIE35_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ4154163.1"
                     /translation="MAESKRVQRLLRVFITLDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3131..3310)
                     /locus_tag="WIE35_001252"
     CDS             complement(3131..3310)
                     /locus_tag="WIE35_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ4154164.1"
                     /translation="MYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYLVVGGQRKG
                     NYGKGHEIAKKLGLK"
     gene            3424..3801
                     /locus_tag="WIE35_001253"
     CDS             3424..3801
                     /locus_tag="WIE35_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4154165.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVAKGAEKKAAENTQVSATHKTA"
     gene            3830..4075
                     /locus_tag="WIE35_001254"
     CDS             3830..4075
                     /locus_tag="WIE35_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154166.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            4309..5277
                     /locus_tag="WIE35_001255"
     CDS             4309..5277
                     /locus_tag="WIE35_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154167.1"
                     /translation="MAKKTTKTIHYLRAQQNHSLFDLEVALRRVLSAAPTVQDTQIEQ
                     YNQLTQIMHRTLDPKDVKGNSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPP
                     SGYSFLRKEAFLYIVGHHVIFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFK
                     AVGNCDKLSLIQQHGVKSILLDASAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSD
                     EELEAQSEIHINLEVLLNGNSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVI
                     QPSDVKLSKSVRIGRYDEANSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            5274..5756
                     /locus_tag="WIE35_001256"
     CDS             5274..5756
                     /locus_tag="WIE35_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154168.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            5846..6349
                     /locus_tag="WIE35_001257"
     CDS             5846..6349
                     /locus_tag="WIE35_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ4154169.1"
                     /translation="MSDKFNQFINRVLSHEGGYANHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAISIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     AAADNAD"
     gene            6458..6790
                     /locus_tag="WIE35_001258"
     CDS             6458..6790
                     /locus_tag="WIE35_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154170.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAAVPQLAEVKKS
                     ANQEGGRQ"
     gene            6787..>7019
                     /locus_tag="WIE35_001259"
     CDS             6787..>7019
                     /locus_tag="WIE35_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ4154171.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGR"
BASE COUNT         1692 a         1648 c         1767 g         1912 t
ORIGIN      
        1 aattaggagg tatttggggc gaaaacagct gaaaacctgt gtttgggttt cggctgtcgg
       61 gagggaaagg aattttgcaa aggtctcaga gtgagtttat tttggggcgg cggcaggtcg
      121 ggggcaagcg gtgtggggct tggttgtggt ttttaggttt ttgggggtaa aaaatgccgt
      181 ctgaactttt cagacggcgt ttgttttttc tgtccaatcg aggaactgcc gccatttttc
      241 cagcggcata tcggcccgcc gcgtttcggt atcgggttcg gcttcccagc ggcctacctg
      301 tatgtagtgc ttcaccccga cgatttgcgc caactcggcc tgtgtcagtt tgcagcggtt
      361 gcgcagggtg cgaaggttgt aaggcgtgta gccgagttcc atgtcgttgc ggtttatttt
      421 gtttcgcata tttttttgac tgcccggcgg caggtttcgg taaggacggc ggcaaatcgg
      481 gcttcgtctg ccggttttcc gtcgaaaaaa ataaaatcgt aaagggtgat gccgttttcg
      541 gaatggcggt aatgcccgcc cgggcattcg ctgtctgtct gcctgtggat tttggcaatc
      601 aggcggggat agcggcaatg ggtaatgaaa cggcttgcgc cgtcttgccc gataatccat
      661 tcggggtagc ggttgaatag tgcggctctg ttcattttgt tcgggttgtt cgggggataa
      721 agcccctcgc ggggcttgtg gtcaggcaaa tttgaatatc agtgccaaca cggcggcgat
      781 ggcggtaacc agcccggtgg ccgccatcat gggataccat cgggattctt gggctatttt
      841 tacggattcg gcgttgattt tgtgcgcgtc tgcaatgatt ttggcgattt cggcatctac
      901 ttttctcaga ctggagtgtt tcatttcttg ttcgattatg ttcatcgtac ttcctttcgt
      961 ttttggcggt tgccgccgct tgtcggatgg taggatgtct gccatgtgta tatattgata
     1021 ccttttaggt tttattgcaa gtgttttggg cggcggcttc gtatgcttgg cggtggcggc
     1081 ggctgtacca ttcggcgagt tcgcgccgct cttggaggcg gtagctgtcg gcggtcaggt
     1141 agtggtgcag gcttgagaag ccggcgcgtt ggagggcggc gcggctgatg tggccgaggg
     1201 cgaggtctgt tgtgagggtg tcttttccgg cggtcaggct gatgttggtg aagaggtggc
     1261 ggaatccgtg cattgtgtgt ttggatttgc cgggggtgct gccgtcatag cccagtcgtc
     1321 ggatggcgtt gtgggcgaat ttgatgctga tgtggtcggg atggggggcg ggtttgcgcc
     1381 gtgggcggat gccggggaaa aggtggatgt tgtcgccggt ctgtgtgtgc agctctcgga
     1441 gtatttctac cgcccagtcc gacagtggga cggtaaaggg gtgtttggtc ttcatgtcgg
     1501 cggcggggat gtgccataac cgggcggtga ggtcgatgtc ttgccagcgg gcggaaagca
     1561 gtgcggaggg acgggatgac ttccccggtt tccccatcaa tccgcgccgc ctcgattttt
     1621 ccgttggcgc gcagcatttc caccgcctgc cgcaccgaca tcaaccgctt gccgttgcgc
     1681 ctcatcgcct ccaccaaaac cgccgaaatc aatttggctt cttccggttt aagctccgtc
     1741 ttgcccgcat cgctgcgccg tttgcgcgtc ggcttgacgc tgaccgcctc cagcttgcgg
     1801 tatagcgtgg caaggctgat gcccaattcc tgcgcctgct gcttaagata tgcagagcgt
     1861 gcgccgcgtt ccattgcttc cgcctgattc tcgactgcct taagacgctc aatcattgcc
     1921 ggattcatcg ccttctcccg tttcaccgcc caaccattcc ggcacattgt ctgtcggtgc
     1981 ttcagtcggc agggcatagc tttcgcgcag ttgctcgcag tccaaaataa tttgattgag
     2041 cgtgccgacc atctttgcct gatggctgat cccgtgtgcc tcactgtgcg cattaagttg
     2101 gtcgaacaaa tctttcagac ggctcacttg actgcggata ccgacctcaa ggcttgttaa
     2161 ctgcatcgcc aactcgctgc ctacgtcttc cgccttcggc tctctgacaa cggtttgctt
     2221 cttagccagc ttctcggcca gctcatcaat tttggctgtt ttggttttca tcacttcgtc
     2281 tttggcggcg aggttttcgc ggctttcgcg cagggcgacg cgcagctcgc gcaccgtcat
     2341 tcggtccaca tcgtcaaagg tcatgccgtt gacttcttcc ccttcggcca aacccaccag
     2401 cgtaacgtct tcttcgacca agagttccag cagcttcgac ttgcccaaat ccatcagctt
     2461 cggcgcggct ttctgcattt gcggggtcgc aaagcggcga gtggctgaca tcagacgtga
     2521 tgtttctgcg atacccagcc caaattggct tttcacaatt tccataaacc gtccgtgttc
     2581 cgtatgctct tttaaaataa tcagcgcacg tcccagttcg aacatgcctt ccatcgtctg
     2641 ccgtactgct tgacgaccgc gttcaaccca acgctcttcg ctataagtct cgccgttacc
     2701 ccactgctcc atcaccagca cactgcgcat cgctgcctga ttgcttactt tgtcggttgc
     2761 gataatttca atttctgtat tcatttttta tctccaaagt ttccgacgtc ggaaactttc
     2821 aaaatccgtt aatccacatc gacccgcttg ccgatttcgg caatcttgct ttgcagccgt
     2881 tcatgctgct gcctgaaccg ctctgcgatt tgcagggttt tgatgccgta ggcgtagttg
     2941 ccgttttcaa gtttgatgac caatcccgag gcaaccaaat catcaatatc cctgctgact
     3001 tgcgatggcg tcagccccag tccgaccgat aaatccttat tgctcagacc gataatcgga
     3061 tgctcgtcaa gcgtgataaa gaccctcaat agccgttgta cccttttact ttctgccatc
     3121 cgcatcctcc ttatttcagt cccagcttct tggcaatttc atgccccttg ccgtaattgc
     3181 ctttgcgctg tccgccgacc accagataca catcgcgcgg cttaaagccg ttctctcttg
     3241 cccactgcgc cagcgtttgg ccgttttggg caaaattttc ttttagtttt tcaacagtat
     3301 agccatagat agccctttcc cattgtgata aaatagtgaa ctatttatgc actagaaatt
     3361 aattagtgca ttaccaatgc gtgaattatg agattttaaa atctctttta caaggattat
     3421 tttgtgattt ctaaatctct ttttggtaat cgtttgaaag aaaaaagaaa gtttttaggg
     3481 cttacccaag cacaagctgc tgaaaaagca ggtattgagc gcgaaacatg gggcaaatac
     3541 gaacgtggcg tatttatgcc tagtggagat gtattgctct cttttttgaa tatgggaata
     3601 gatgtgagca gcttatttgc tgctgaacag ggaattaggc cgtctgaaaa ttctgaaatt
     3661 agtaaggaag agtcagagtt attagggtat taccgccaag ccagcgataa cggcaaattt
     3721 gtcattctca gtgtggcaaa aggcgcagag aaaaaagcag ccgaaaatac tcaagtttca
     3781 gccacgcata aaacagcgtg atttttaaat ccaaaatgga gacagtctta tgtttgactt
     3841 tgaacaacaa atcaaatggg gtgagcgagc ggaagaaatc gttaaagagg cagccaccca
     3901 gaacaatatc gaaattcccg aacctttggc ttcggcatta gctaaggcgg taaaagtgca
     3961 ttatctaagc caagctggtg tgttttcttt ggtagaagcc tatgcagaca cggtaaatcc
     4021 aactgaaaaa gaagtagatt accaagcaat cggcaaagag ctatttgaaa aataaaaaat
     4081 gccgtctgaa attttcagac ggcatttagg acactccgac agtcgggctg ctccggtgtt
     4141 ctgaaacgga tattaagcaa ttgaagcggc cgtgcatttt aaaacgcatt aaaaactacc
     4201 ccgacataca ggtcatcggg caggtggttc aggtgttgaa agatttgaat tgatgccgtc
     4261 tgaatttatt ttttcgacac agatattaaa agagaaagga ctgtacaaat ggcaaaaaaa
     4321 actactaaaa ctattcatta tttacgtgcg cagcaaaacc atagcttgtt cgatttagag
     4381 gtagcattaa gaagggtttt gtcagctgct cctactgttc aagatacaca aattgaacaa
     4441 tacaatcagc ttacccaaat catgcataga actttggatc ctaaagatgt aaaaggcaat
     4501 tcaattggtg gattactgat acacattggc agcggtacaa aagatgaaca tatccgcact
     4561 atgagcaata gacctgtaca gcaagatgat catggtggaa cacaagctcc tccaagtgga
     4621 tattcctttt tgcgtaaaga ggcttttttg tatattgttg gacaccatgt aattttttgc
     4681 ggacatggtt ttttgtcagc atcaacggtt gcttcttatt taagtttact tagcaataaa
     4741 ttgagagaga acaatccgga tattgttcaa tttaatattg aattcaaagc agtaggcaat
     4801 tgcgacaagt tgtcattgat acaacaacat ggggtaaaaa gtattctttt agatgcttcg
     4861 gcatatcaat tatccagaaa cagactgtat caagatagcc gttcaaccat tgctaaagca
     4921 ttagggaaag ttggtagtgt atttacaagc gaattgagtg atgaggaatt ggaagcacaa
     4981 tccgaaattc atatcaattt agaagtcttg ctaaacggta attcgagagc cagtattgaa
     5041 gcgcaatcgc tgatgcagga gcaggcagag gaaattattg atgatgaaac ggttaatcaa
     5101 ggattttcca ttaccacgca acaaggtgaa gtgattcagc cctctgatgt aaaactgtct
     5161 aaatctgtta gaataggacg atacgatgaa gccaactcgt tactccctga ctcggcattt
     5221 acagcaatca gtgaatattt cctagaactc caaagcagaa acttaactga acaatgaaaa
     5281 aatatctgcc cttactacgt tttttgttga ttgcttgtat atcagcattt ttagcatgga
     5341 aagggcaacc ttttgttcat gggaacgaga aggccgttga tttaattatt aacgtatttg
     5401 caatcttagc cggtttttta attgccatta tgacattgtt tagtgatatg aggtttgatg
     5461 aagatgcaaa ttggcgacaa attcaaattc gtgaaggcgt acaagaacaa cggtatataa
     5521 aacattcctt acttttttac acatatcttg ccgtattagt ttgtgttttt attgtcatct
     5581 tgttggctca taaagaagaa tacaaaaatg gaccagccat tttttggctt gaacggagct
     5641 atttattttt agcctgtatt tccatttttt attctgtatt cttacctggg aatttaatta
     5701 aaagccgaaa agaagagttt aaaaaattaa tggaaaagaa gaaacccaaa atctagaacg
     5761 agttttaacc ccctttaaaa tcccattcag acgacctttt ctaaaatccc tgtattgatt
     5821 tttaattcaa tacagggatt tttccatgtc agacaagttc aaccaattca tcaaccgcgt
     5881 cctctctcac gagggtggtt acgccaacca tcccaaagac cccggcggcg aaaccaattg
     5941 gggcatcact aagcgcaccg cacaggcaaa cggctacaac ggctccatgc gtgccatgac
     6001 gcgtgaacag gcaatcagca tttaccgtaa agcgttttgg gagcgttacc gcgccgacca
     6061 aatgcccgaa gcggtcgctt ttcaattttt tgatgcctgc gtcaaccacg gttacggcaa
     6121 tgccgcccgt atgctccaac gcgccgcagg cgtgccggac gatggaatca tcggcgaaat
     6181 cagcctcaaa gccatcaatt cccttcccga aaacgacctt ttattgcggt tcaacgccga
     6241 gcgtctggtc ttttatacca agctgggtac gttcacctct ttcggcaagg gctgggtacg
     6301 ccgtgtggcg caaaacctga ttcacgccgc cgcagacaat gccgattgag gagcaaacca
     6361 tgtcaaaaaa gtccattctc gccctgatgg ccgcagccat gcagcccgat ttcagccaca
     6421 gcgacctagg cattcgctac gccatgccga ctcagggatg ttggacgcaa gcccaccgca
     6481 agagcggggt agccgccgcg aaacgcgcag ccaaaaaaaa gcgtcacaaa taaccgcctt
     6541 tttccgatgg ctgggcggct tggtctctaa tccggccaca gggaaaatca gccataccaa
     6601 actttgggca aacgtcgccg cagccgccat gacttggaag ttcgtgcagg cggcggacgc
     6661 gcccgaatgg ctttggtggg catacggcgc gatggtcggc gggtatgcat taatcaaacg
     6721 cggcatcgcc gccgtaccgc agttggcgga agtcaaaaaa tccgcaaatc aggaaggggg
     6781 gcggcaatga ttgaatttgt ccgagccaaa aaacggctgc tttgggcatt tgtgcttttg
     6841 cttgtgtgga cgtgcggtta ccgatacgcc gccgacaagg ccgaagcgga acaaaccgcc
     6901 ctgattgcca cctatcggca ttcttctatg gttgcggcgg aacaatatgc cttgcagctt
     6961 aaaaaagcgc aggacgaaag gcagcggtgg tacgactttt cccaaaaaca aggaagaaa
//
LOCUS       DAWHXZ010000096         6998 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000096 DAWHXZ010000000
VERSION     DAWHXZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.096"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            366..665
                     /locus_tag="WIE35_001260"
     CDS             366..665
                     /locus_tag="WIE35_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ4154172.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFLFAVLTVLKLKGYQAGLYTLLIALAVAAFGFGMPAGMAVSSLPPQPH"
     gene            605..841
                     /locus_tag="WIE35_001261"
     CDS             605..841
                     /locus_tag="WIE35_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ4154173.1"
                     /translation="MRLRDAGGYGGFFPAAAAALTQRPYATLDLTAHYKIGKSTRIGL
                     DFENVFNKRYRPMPDIHVYGTPRSLTATVKHIVD"
     gene            complement(1033..2436)
                     /gene="mtrE"
                     /locus_tag="WIE35_001262"
     CDS             complement(1033..2436)
                     /gene="mtrE"
                     /locus_tag="WIE35_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ4154174.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREATYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALTAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(2491..5694)
                     /gene="mtrD"
                     /locus_tag="WIE35_001263"
     CDS             complement(2491..5694)
                     /gene="mtrD"
                     /locus_tag="WIE35_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ4154175.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIASKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSLEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(5706..6944)
                     /gene="mtrC"
                     /locus_tag="WIE35_001264"
     CDS             complement(5706..6944)
                     /gene="mtrC"
                     /locus_tag="WIE35_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ4154176.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEANLESARAQLVTAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPAVDE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQADVQTASEAKTASEAE"
BASE COUNT         1625 a         1961 c         1831 g         1581 t
ORIGIN      
        1 ccgtactggt ttttgttcat ccactataca tcaaacatca aattggactg accaatcagg
       61 gcggattcta atgacacgcc gttcccgccg tcaacggcat ttacctcgca ccgcccccga
      121 aacacaggaa aaaactacac caaactacaa tttttgttta tgcaaatatt tgttttgaca
      181 ggatttaaac gaaagctccg attcaaatct gccgaaccgc ccaaaaatat atagtggatt
      241 aacaaaaatc aggacaaggc gacgaagccg cagacagtac agatagtacg gtaaggcgag
      301 gcaacgccgt actggtttaa atttaatcca ctctattgac ctgaatatta aagtttcgca
      361 aagtaatgca acgttgcttt aattggtttg accactattg ccgacgatta gaaaaatatt
      421 ttcggagatg ttcaattatg gaaacttggg ttcaaaacta cacggcaatc ggcggcagcc
      481 tgtatctgac tgccgccgcc gcactcttac ccatcgtctt tttatttgcc gtgctgaccg
      541 tcctgaagct gaaaggctat caggcggggc tttatacgct gctgattgcg cttgccgttg
      601 ccgcattcgg cttcgggatg ccggcgggta tggcggtttc ttccctgccg ccgcagccgc
      661 actgacccaa cgcccctacg ccacgctgga tttgaccgcg cattacaaaa tcggcaaatc
      721 cacccgcatc ggtttggact ttgaaaacgt gttcaacaaa cgctaccgcc ctatgcccga
      781 cattcacgtt tacggcacgc cgcgcagcct gaccgcaacc gtcaaacata tagtggatta
      841 acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacaa cttggtgctt
      901 cagcacctta gagaatcgtt ctctttgagc taaggcgagc caacgctgta ctggtttttg
      961 ttaatccact ataaagaaag aaatgccgtc tgaaacctta tcgtttcaga cggcatttta
     1021 ttgcccgacc ggttatttgt cggtttgggt atcccgtttc aatccgccgc cgagtgcctt
     1081 gtacaaatcg gcaaggtttt cggcgcgggt cagttgtgcc gacaaagccg caccttccgc
     1141 cgaatagctg ctgcgttccg catcgagcaa atcaagcgcg ccggatacgc cgtgtttgta
     1201 acgcagaccg accaaacgca acgcttcttt agaggcgcgg ctttgtttgc ttaaagcatc
     1261 ataggcttta tccaactgct cgcgcgcggt caatgcgttt gccacgtctt gaaatgcgga
     1321 ttggacggcg gattcatagg caacgatttg tgcctgttgg cgcagttttg ccacatcgag
     1381 gttcgccttg ttcgtacccc aggtaaaaat cggcagggta atggaaggcg caaacgacca
     1441 aacgcccgtg ccgcttttga acagcccgcc caattcggca gaacccgtac caacgcttcc
     1501 ggtcaggcgg atggatggga aaaaggcggc gcgtgccgca ccgatattgg cgtttgcctg
     1561 tttgagcgcg tgttcggcag cacggatatc gggacggtcg agcaatactt cggaactcaa
     1621 accggccggc agtttttcaa caaaaaactg cttgtccaac ggcaaaccgg cgggcaggtc
     1681 gtcgggtatc ggttggttaa tcaaggttgc caaggcattg cgcgcctgtt cgcggctgcg
     1741 cgcggcatgg gcataatcgg ctttggcgga ttcgatgagg gcttcctgct ggcgtaaggc
     1801 gacggcggaa atcacgcctg ccttgtaacg caattcagac agcttgtagg ttgcttcgcg
     1861 cgttttcaag acacgctgcg ccaaagacat cgcttcttcg gcataacgtt cgttgaaata
     1921 ggctttggca acggtggcaa tcaggctcaa atgtgccgca tcgcggttgg cggtgctggc
     1981 aaaatagcct tgcagtgctg cctcgctgct gctgcggacg cgtccgaaca ggtcgagttc
     2041 gtaagatgcc gcacccagtc cgactttgta gctgctgctt acattgccgc cgctcaagct
     2101 gccttggcgc gagtcgttcg cattggcggc aagcgtgggc aggaggttgt tgcgctcaat
     2161 catgtattgt ttgcggtaga tttcgctgtt caatacggcg gtacgcaaac tggtattgcg
     2221 ctcgagtgcg atgtcgatca gcttttgcag gcgcgggtcg gcaaaatagt catgccaacc
     2281 taaatcgacc gcacggatgc cgctgtcggc ggtatcgttt ttgaacgttt cggcaacttc
     2341 gactttgggc tgctcgtatt ggggaatcat ggtgcaggcg gataatgcga aggctgctgc
     2401 aacagaagtc aaggtggttt tcaatgtagt atccataaaa aagtcctgat gccgtctgaa
     2461 aacccgtggg cgttcagacg gcatggttgc ttaatgttgg ctgtcgtccg aaccggtgat
     2521 gcccgcttcg gcggcgtgtt ttactgccat ttcgtgttcg tgcgcggttt ctttgaagaa
     2581 tttgcgcacc accacataga aaagcggaac aaggaacacg gacaagagcg tgccgatgag
     2641 catcccccag aatacggtcg tgccgatggc gcgctggctg gcagaacttg caccaccggc
     2701 aatatacagg ggaaccacgc ccaaaataaa ggcgaacgag gtcatgataa tcggacggaa
     2761 acgcaggcgg gctgcttcca aagcggcttc aaccgcgctt ttcccttgcg cttgaaggtc
     2821 tttggcaaac tcgataatca aaatcgcgtt tttcgcactc aaacccatca cggtaacgaa
     2881 accgacttga aagtagatgt cgttggcgaa cgagggaacg ctgcccaaca gtccttcaaa
     2941 caggttgcgc ccggttacgc ccgcagccgc accgatcaaa cccaacggaa tcacaaggat
     3001 gaccgccagc ggaatcgacc agctttcata aagcgcggca agtaccaaaa atacggctgc
     3061 aaccgccaaa ccgtacaaaa tcagggtttg cgagccgcct tttgcctctt cgcgcgactg
     3121 tccgccccac tccaggctgt aaccgccgcc caattcgtca accatttttt gaaccgccgc
     3181 catagcctgc ccggtggaaa cgccggttgc aggcgaagcg gacagcttca tcgaaggata
     3241 accgttgaag cgtacgctct gttccgtacc gttttcccaa gaaacagtag caatggtgga
     3301 aagcggtacg gcgacgccgg atttgttcgg cacggtcagg ttcaaaatat cggcaggctg
     3361 catacgggca tcctcgtcgg cctgcaccat cacgcgttgc agacggcctt ggttcgggaa
     3421 gtcgctgaca taagacgaac tcagcgcact tgccaatgcg gtgcggatgt cggcaaacga
     3481 aatgccttgc gccgccgcgg cggcacggtt gatgtcgatt ttcaactgcg gcgagtcttc
     3541 caaaccgccg gcacggacgg tgctggggtc aaacaaaccg ctggcacgca ttttctgaat
     3601 caactcgttg cgcttcgcca gcaatgcggt atggccggta ttgttgcggt cttgcaggtt
     3661 gatgctcaga cccgaaccgt tgcccaactc cagaatcgga ggcgggacga cggcgatgcc
     3721 aaaaccgtct ttaagcgtcc ccatcatcat acccgtcagc ttgctggcaa tcgcaacggc
     3781 atcgctgccg ggcgcggtac gctcgttcca atctttcaat atggcaaaac ccatcgccat
     3841 attctgaccg ctgcccgaaa agctgaagcc ggaaacggta atgatgtttt ctatttcagg
     3901 aatgcttttc gccagttggg taacttgcgc caaagtcgca ttggtgcgct cttgggtcgc
     3961 tcctgcaggc agttgcacgc tgaccatgac gaagccttgg tcttcggtcg gcaggaacga
     4021 agtcggcagg cgcataaaca ggaacacgcc cacaaccgcc aagccgatat agacaaccat
     4081 catgcggaaa gtcttacgca gcactttggc aacccggcct tcgtaaccgt gcgtccaact
     4141 gttgaatttc ttgttaaacc agccgaagaa accttttttc tcttcgtgat gccctttcgg
     4201 gattgtcttc aacattgtgg cacacaaagc aggggtaagg gtcagcgcaa ggaaggcgga
     4261 gaatgcgatt gatgacgcca tcgtcagggc aaactgtttg taaatattgc ccgtcgcccc
     4321 gctgaacatc gccaacggta cgaacacgga aatcagaacg gcggtaatac cgatgaccgc
     4381 gcccgaaatc tgacccatcg cttttttggt cgcttctttg ggcggcaagc cttcacccgc
     4441 cataatgcgc tcgacgtttt caaccaccac aatcgcgtca tcgaccacga tgccgatgac
     4501 caaaaccatc gcaaacatgg tcagtacgtt aatcgacatg cccatataag agatgaaagc
     4561 gaaaccgccc aacagcgaaa tcggtacgac gatggtcgga atcagcgtat aacggatgtt
     4621 ttgcaggaag agatacatta cgacaaacac cagcaccatc gcttcgatta aagtatgaat
     4681 cactttttca atcgaaattt cgacgaattt ggaagtatcg taaggggttt tccagctcat
     4741 accctgagga aagtattttt ccaacgtcgc catgcgttct ttaaccgcct ttgccgtcgc
     4801 catcgcattg ccgctgttgg acagcatcac cgccataccg gtggtattta caccgttcag
     4861 acgggttgag gaagaatagt cttccatacc cagtccgacc cttgccacat ccttcaggta
     4921 aacattagaa ccgtcggtat tggcgcggag gatgacgttg ccgaattctt ctgccgtacc
     4981 caactgccct tgcgccgtta cggtagccgt aaccgtctgt ccgcgaacgg cgggaagcga
     5041 accgatagaa cccgctgaaa tctggacgtt ctgggcggac agcgcgctgc cgatatcggc
     5101 aaacgacaaa ttgtagtttt gcagtttctt aggatcaacc caaatccgca tcgcgcgttg
     5161 cgcgccgaac aggcgtacct gccccacgcc ttcgatacgc tgcagttcgg gaacgatatt
     5221 acgctgcgcg tagtcgttca tctcttcggt tgactgcaca tccgacgaaa gcatcacaat
     5281 catcaggaaa ttggaacgcg ccttggatac ggttacgccg tattgctgga cggttgccgg
     5341 cagcgtgctc aatacttcgg aaagcttgtt ctgcacttcc acctgcgcca gattctcgtc
     5401 ggtatcgggc gtaaaggtca ggctcacgct gccgctgccg ctcgaatcgg cggaagtgga
     5461 catataatcc aaaccttcca cgccgttcat attccgctcg atcacggaaa gcacgctgtc
     5521 ttccattacc tgcgcggacg cacccggata agtggcgtgc aaagtaatgg tcggggcggc
     5581 gacggacgga tattgcgaaa caggcaggct tttgatgccg aaaatacccg ccgcaataat
     5641 gaaaatcgaa ataacccacg caaaaatagg gcggtcgata aaaaatttag ccatcgatgc
     5701 cttccttatt ccgcttcaga agcagttttg gcttcagatg ccgtctgaac gtcagcttga
     5761 ggcgcggcgg cttggttttc agacggcgcc cattctttgg gcgttacctt tttcgcaccc
     5821 gttataccgg cgatactgat gccttccaca accaccttgt ccccgtcctt cagacccgac
     5881 gtaacaatcc aattcgtacc ctgctgttgc gcgaccgtta cctcgcgggg ttccataccg
     5941 ccttgggcat tcacaatcat cacggtatct ttcgcaccgc gcgttaccgc ctgctgcggc
     6001 acaacaaatg cgttatccac cgccacttgg tccatcagca cgcgcacata caggccgggc
     6061 atcaggatgt tctgatcgtt cggtacggcg gcgcgcaggg taatctgacc ggtcgattcg
     6121 tcaacggccg gatcggcaaa cagcaggcgg cctttttcag ggtaaactgt gccgtcgtca
     6181 aatttgatgc cgaccgcaat cacaccatcc gccgccagca gtttgccttc ggctatctga
     6241 cggcgcaatt tcatcacttc ggatgcagac tgggtaacgt tcacatacat cggattggtt
     6301 tggcggatgg ttgccagcac ggtcgtatcg cccgcattca acagcgtacc ttcggaaact
     6361 ttggactgac cgataaagcc ggaaatcggc gcggtaatgc gcgaacggtt cagattgatg
     6421 ccggcagatt tgatcgccgc ctgtgctgct ttgacacctg cctcggcaga acgtttcgcc
     6481 gttaccgcag catcgtattc ctgccggctg acggcttcgg cggcaaccaa aggcttgtat
     6541 cgcgccaaat ccgcatccgc tttggcaagc gttgcctgag ccgttaccag ttgcgcgcgc
     6601 gcgctttcca gatttgcttc ataagtggaa ctgtcgatct gatacagcgg ctgtccggca
     6661 cggacataac tgccttcttg gaacaggcgt ttttggataa tgccgccaac ttgcgcacgg
     6721 acatcggcgg tacgcagcga ttccaaacgc cccggcaact cgacggtcaa tgcgacggtt
     6781 tgcggatgga cggttacgac accgacgacg ggcgcagggg cttcccgacc agcaggctgc
     6841 ccgccctgcg ccgcgtctcc gcctttaccg caagacgaca gtaccaatgc aacggcggca
     6901 gccaacgcgg ccgcacgcat cgccttaaaa gcataaaaag ccattattta tcctatctgt
     6961 ctggtttgat gtaaagggtt ttgccaatca acaggcat
//
LOCUS       DAWHXZ010000097         6951 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000097 DAWHXZ010000000
VERSION     DAWHXZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.097"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(78..1424)
                     /gene="mnmE"
                     /locus_tag="WIE35_001265"
     CDS             complement(78..1424)
                     /gene="mnmE"
                     /locus_tag="WIE35_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ4154177.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGVNDKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1555..1710
                     /locus_tag="WIE35_001266"
     CDS             1555..1710
                     /locus_tag="WIE35_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154178.1"
                     /translation="MEKRNTYAFLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1707..2138
                     /locus_tag="WIE35_001267"
     CDS             1707..2138
                     /locus_tag="WIE35_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ4154179.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGS
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2272..6642
                     /gene="app"
                     /locus_tag="WIE35_001268"
     CDS             2272..6642
                     /gene="app"
                     /locus_tag="WIE35_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ4154180.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFTYKIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGRKYIDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPRQNGKYSFHD
                     NNNGTGKIDAKHEHNSLPNRLKTRTVQLFNVSLSETAQEPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDIATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKA
                     ENFQIKGGQAVVSRNVAKVKGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEK
                     TITDDKVIASLTNTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNG
                     DLSLVGNAQATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSHSALNG
                     NVSLADKAVFHFESSRFTGQISGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSATDAPRRRSRRSLLSVTPPASAESHFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAGKDNAQSLDALIAAG
                     RDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETQPATTAFP
                     RARRARRDLPQPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAE
                     DRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTF
                     DDGIGNSARLAHGAVFGQYGIGRFDIGISTGAGFSSGSLSDGIGSKIRRRVLHYGIQA
                     RYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQ
                     HISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAA
                     AKGPQLEAQHSAGIKLGYRW"
BASE COUNT         1856 a         2008 c         1684 g         1403 t
ORIGIN      
        1 accaaacgga tcggattccc gtttttatgg gataacggaa tgttcagccg gacaccgcca
       61 cgatgctttc gatccgttta ttttccgata caaaacctcg aaaaaatcac gccgagcagg
      121 tcgtccgccg taaactcgcc cgtgatttcg ccgcatgcga cctgcgccaa gcgcaagtgt
      181 tcggcaaaca gctcgatttg atggttgccg cacaatgccg ccagcgacaa ttcttcctgc
      241 gctgctttga gtgcgttgac gtgccgcgtc cgcgccaaaa acagcccttc gccctcgccc
      301 tgccatccgg cctcgcgcaa caacgtccgt ttcagcgcgt ccaagccgtc gccggttttc
      361 gccgacagcg cgatgacggt ttccgcgccc gtaccgaacc cgcctgccgc gtgtgcgtgc
      421 aaatcggatt tgctgtggat ttcgatgcgt ttcaactccg gcggcaacgc gtccaaaatc
      481 gcccgtgtct tgtcgttcac gccttcgcgc ggatcgacca acaccagcgc gacatcggct
      541 tcggatacgg ctttgcggct gcgttcgatg ccgatgcgtt cgaccacgtc gtccgtctcg
      601 cgcaaacctg ccgtatcgac aatatgcacc ggaacgccgt caatcaggat acgttccctg
      661 accgcgtcgc gcgtcgttcc ggcaatatcg gtaacaatcg ccacttcgtc gcccgccagc
      721 gcgttcaaca ggctggattt gccgacattc ggcgcgccga ccaatacgac attcagacct
      781 tcgcgcaaaa tcgcgccctg ctgcgcgttg gcaagcacat catccacggc gcggcgcaag
      841 ccgtccagtt tgccgcgtgc gtctgccgct tcgagaaaat caatgtcttc ctcgggaaaa
      901 tctaacgtcg cttcgaccag catccgcaag gtaatcaagt cttcgaccag accgtgtatc
      961 cgccgcgaaa aatcgccttt gagcgagcgc aacgccagac gcgccgccga acggctggat
     1021 gcgtcaatca aatccgccac gccttccgcc tgtgccaagt ccagtttgtc gtttaaaaac
     1081 gcacgcttcg taaactcgcc cggttcggca aggcgcgcgc ccaattccaa acagcggttc
     1141 agcagcatat ccatcactac cggcccgccg tgtccctgaa gctcgataac atcttcgccc
     1201 gtaaaacttg ccggtgcggc aaaaaaaagc aaaagcccgc tgtcgattgc ctgaccgtcc
     1261 gcgtccgtaa aatcagcata ggttgccacg cgcggcttgg gcgttttccc acacaaagcc
     1321 tctgccatcg gcagcaggtt tttccccgat atgcgtatca cgcccacgcc gccgcgcccg
     1381 ggtgcggtag cgactgccgc aatcgttgga acgttatccg acataaaacc cccgaaaatt
     1441 caaaacagcc gcgattatag caaatgccgt ctgaagtccg acggtttggc tttcagacgg
     1501 cataaaaccg caaaaatgct tgataaatcc gtccgcctga cctaatataa ccatatggaa
     1561 aaacgaaaca catacgcctt cctgctcggt ataggctcgc tgctgggtct gttccatccc
     1621 gcaaaaaccg ccatccgccc caatcccgcc gacgatctca aaaacatcgg cggcgatttt
     1681 caacgcgcca tagagaaagc gcgcaaatga ccgaaaacgc acaggacaag gcgcggcagg
     1741 ctgtcgaaac cgtcgtcaaa tccccggagc ttgtcgagca aatcctgtcc gacgagtacg
     1801 tgcaaataat gatagcccgg cgtttccatt cgggatcgtt gccgccgccg tccgacttgg
     1861 cgcaatacaa cgacattatc agcaacgggg cagaccgcat tatggcaatg gcggaaaaag
     1921 aacaagccgt ccggcacgaa accatacggc aagaccaaac cttcaacagg cgcgggcaac
     1981 tgtacggctt catcagcgtc atcctgatac tgctttttgc cgtcttcctc gtatggagcg
     2041 gctaccccgc aaccgccgcc tcccttgccg gcggcacagt ggttgccttg gcgggtgctt
     2101 tcgtgattgg aagaagccga gaccaaggca aaaattaatg acaaatccta gggcgtgctt
     2161 catatccgcc cgaacgccga accgcacata taggcacatc ccgcgcgccg cccggaagcg
     2221 gaagccgcgc cctcccaaac aaacccgaat cccgtcagat aaggaaaaat aatgaaaaca
     2281 accgacaaac ggacaaccga aacacaccgc aaagccccga aaaccggccg catccgcttc
     2341 tcgcctgctt acttagccat atgcctgtcg ttcggcattc ttccccaagc ttgggcggga
     2401 cacacttatt tcggcatcaa ctaccaatac tatcgcgact ttgccgaaaa taaaggcaag
     2461 tttgcagtcg gggcgaaaga tattgaggtt tacaacaaaa aaggggagtt ggtcggcaaa
     2521 tcaatgacaa aagccccgat gattgatttt tctgtggtgt cgcgtaacgg cgtggcggca
     2581 ttggtgggcg atcaatatat tgtgagcgtg gcacataacg gcggctataa caacgttgat
     2641 tttggtgcgg aaggaagcaa tcccgatcaa catcgtttta cttataaaat tgtgaaacgg
     2701 aataattata aagcagggac taacggccat ccttatggcg gcgattatca tatgccgcgt
     2761 ttacataaat ttgtaaccga tgcagaacct gttgaaatga ccagttatat ggatgggcgg
     2821 aaatatatcg atcaaaataa ttaccctgac cgtgttcgta ttggggcagg caggcaatat
     2881 tggcgatctg atgaagatga gcccaataac cgcgaaagtt catatcatat tgcaagtgcg
     2941 tattcttggc tcgttggtgg caataccttt gcacaaaatg gatcaggtgg tggcacagtc
     3001 aacttaggta gtgaaaaaat taaacatagc ccatatggtt ttttaccaac aggaggctca
     3061 tttggcgaca gtggctcacc aatgtttatc tatgatgccc aaaagcaaaa gtggttaatt
     3121 aatggggtat tgcaaacggg caacccctat ataggaaaaa gcaatggctt ccagctggtt
     3181 cgtaaagatt ggttctatga tgaaatcttt gctggagata cccattcagt attctacgaa
     3241 ccacgtcaaa atgggaaata ctcttttcac gacaataata atggcacagg aaaaatcgac
     3301 gccaaacatg aacacaattc tctgcctaat agattaaaaa cacgaaccgt tcaattgttt
     3361 aatgtttctt tatccgagac agcacaagaa cctgtttatc atgctgcagg tggtgtcaac
     3421 agttatcgac ccagactgaa taatggagaa aatatttcct ttattgacga aggaaaaggc
     3481 gaattgatac ttaccagtaa catcaaccaa ggtgctggag gattatattt ccaaggagat
     3541 tttacggtct cgcctgaaaa caacgaaacg tggcaaggcg cgggcgttca tatcagtgaa
     3601 gacagtaccg ttacttggaa agtcaatggc gtggcaaacg accgcctgtc caaaatcggc
     3661 aaaggcacgc tgcacgttca agccaaaggg gaaaaccaag gctcgatcag cgtgggcgac
     3721 ggtaaagtta ttttagatca acaagcagat gaaaataata aaaaacaagc ctttagtgaa
     3781 atcggcttgg tcagcggcag gggtacggtg caactgaatg ccgataatca gttcaacccc
     3841 gacaaactct atttcggctt tcgcggcgga cgtttggatt tgaacgggca ttcactttcg
     3901 ttccaccgta ttcaaaatac cgatgaaggg gcgatgattg tcaaccacaa tcaagacaaa
     3961 gaatccaccg ttaccattac aggcaataaa gatattgcta caaccggcaa taacaacagc
     4021 ttggatagca aaaaagaaat tgcctacaac ggttggtttg gcgagaaaga tacgaccaaa
     4081 acgaacgggc ggctcaacct tgtttaccag cccgctgcag aagaccgcac cctgctgctt
     4141 tccggcggaa caaatttaaa cggcaacatc acgcaaacaa acggcaaact gtttttcagc
     4201 ggcagaccaa caccgcacgc ctacaatcat ttaaacgacc attggtcgca aaaagagggc
     4261 attcctcgcg gggaaatcgt gtgggacaac gactggatca accgcacatt taaagcggaa
     4321 aacttccaaa ttaaaggcgg acaggcggtg gtttcccgca atgttgccaa agtgaaaggc
     4381 gattggcatt tgagcaatca cgcccaagca gtttttggtg tcgcaccgca tcaaagccac
     4441 acaatctgta cacgttcgga ctggacgggt ctgacaaatt gtgtcgaaaa aaccattacc
     4501 gacgataaag tgattgcttc attgaccaat accgacatca gcggcaatgt cagccttgcc
     4561 gatcacgctc atttaaatct cacagggctt gccacactca acggcaatct tagtgcaaat
     4621 ggcgatacac gttatacagt cagccacaac gccacccaaa acggcgacct tagcctcgtg
     4681 ggcaatgccc aagcaacatt taatcaagcc acattaaacg gcaacacatc ggcttcgggc
     4741 aatgcttcat ttaatctaag cgaccacgcc gtacaaaacg gcagtctgac gctttccggc
     4801 aacgctaagg caaacgtaag ccattccgca ctcaacggta atgtctccct agccgataag
     4861 gcagtattcc attttgaaag cagccgcttt accggacaaa tcagcggcag caaggatacg
     4921 gcattacact taaaagacag cgaatggacg ctgccgtcag gcacggaatt aggcaattta
     4981 aaccttgaca acgccaccat tacactcaat tccgcctatc gccacgatgc ggcaggggcg
     5041 caaaccggca gtgcgacaga tgcgccgcgc cgccgttcgc gccgttccct attatccgtt
     5101 acacctccgg cttcggcaga atcccatttc aacacgctga cggtaaacgg caaattgaac
     5161 ggtcagggaa cattccgctt tatgtcggaa ctcttcggct accgcagcga caaattgaag
     5221 ctggcggaaa gttccgaagg cacttacacc ttggctgtca acaataccgg caacgaacct
     5281 gcaagcctcg aacaattgac ggtagtggaa gggaaagaca acaaaccgct gtccgaaaac
     5341 cttaatttca ccctgcaaaa cgaacacgtc gatgccggcg cgtggcgtta ccaactcatc
     5401 cgcaaagacg gcgagttccg cctgcataat ccggtcaaag aacaagagct ttccgacaaa
     5461 ctcggcaagg cagaagccaa aaaacaggcg ggaaaagaca acgcgcaaag ccttgacgcg
     5521 ctgattgcgg ccgggcgcga tgccgtcgaa aagacagaaa gcgttgccga accggcccgg
     5581 caggcaggcg gggaaaatgt cggcattatg caggcggagg aagagaaaaa acgggtgcag
     5641 gcggataaag acagcgcctt ggcgaaacag cgcgaagcgg aaacccagcc ggctaccacc
     5701 gccttccccc gcgcccgccg cgcccgccgg gatttgccgc aaccgcagcc ccaaccgcaa
     5761 cctcaacccc aaccgcagcg cgacctgatc agccgttatg ccaatagcgg tttgagtgaa
     5821 ttttccgcca cgctcaacag cgttttcgcc gtacaggacg aattggaccg cgtgtttgcc
     5881 gaagaccgcc gcaacgccgt ttggacaagc ggcatccggg acaccaaaca ctaccgttcg
     5941 caagatttcc gcgcctaccg ccaacaaacc gaccttcgcc aaatcggtat gcagaaaaac
     6001 ctcggcagcg ggcgcgtcgg catcctgttt tcgcacaacc ggaccgaaaa caccttcgac
     6061 gacggcatcg gcaactcggc acggcttgcc cacggcgccg ttttcgggca atacggcatc
     6121 ggcaggttcg acatcggcat cagcacgggc gcgggtttta gcagcggcag tctttcagac
     6181 ggcatcggaa gcaaaatccg ccgccgcgtg ctgcattacg gcattcaggc acgataccgc
     6241 gccggtttcg gcggattcgg catcgaaccg cacatcggcg caacgcgcta tttcgtccaa
     6301 aaagcggatt accgctacga aaacgtcaat atcgccaccc ccggccttgc gttcaaccgc
     6361 taccgcgcgg gcattaaggc agattattca ttcaaaccgg cgcaacacat ttccatcacg
     6421 ccttatttga gcctgtccta taccgatgcc gcttcgggca aagtccgaac gcgcgtcaat
     6481 accgccgtat tggctcagga tttcggcaaa acccgcagtg cggaatgggg cgtaaacgcc
     6541 gaaatcaaag gtttcacgct gtccctccac gctgccgccg ccaaaggccc gcaactggaa
     6601 gcgcaacaca gcgcgggcat caaattaggc taccgctggt aaccgccgga tatgccgaaa
     6661 ggggtctgac gatgccaccg tgcgctgtca aacccttttt ctgccgccgc cttgtgcctg
     6721 ctccattgtc tgataaccgt caaattgatt tttagcccat gtttggtggg tcggggaaat
     6781 ctatatcttc gtccgtgccg aaatagtctg agacctttgc aaaattcctt tccctcccga
     6841 cagccgaaac ccaaacacag gttttcgtct attttcgccc caaatacctc ctaattctac
     6901 ccaaataccc ccttaatcct ccccggacac ctgataatca ggcatccgtg c
//
LOCUS       DAWHXZ010000098         6945 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000098 DAWHXZ010000000
VERSION     DAWHXZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.098"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1044
                     /gene="mafB"
                     /locus_tag="WIE35_001269"
     CDS             <1..1044
                     /gene="mafB"
                     /locus_tag="WIE35_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ4154181.1"
                     /translation="LKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPIKGIGAVRG
                     KYGLGGITAHPVKRSQMGEIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRY
                     GKENITSSTVPPSNGKNVKLANKRHPKTKVPFDGKGFPNFEKHVKYDTKLDIQELSGG
                     GIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQREWENKT
                     GLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWE
                     MKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAWEKRKELEGQKWRGTSKSGVEIEGFT
                     EPNRTAYPLYNSK"
     gene            1071..1490
                     /locus_tag="WIE35_001270"
     CDS             1071..1490
                     /locus_tag="WIE35_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154182.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            1568..1651
                     /locus_tag="WIE35_001271"
                     /pseudo
     CDS             1568..1651
                     /locus_tag="WIE35_001271"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1648..1890
                     /locus_tag="WIE35_001272"
     CDS             1648..1890
                     /locus_tag="WIE35_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ4154183.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            1968..2213
                     /locus_tag="WIE35_001273"
     CDS             1968..2213
                     /locus_tag="WIE35_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ4154184.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PNYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            2216..2605
                     /locus_tag="WIE35_001274"
     CDS             2216..2605
                     /locus_tag="WIE35_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154185.1"
                     /translation="MWKIIKEDSNDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSCSLSKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPPL"
     gene            2632..2823
                     /locus_tag="WIE35_001275"
     CDS             2632..2823
                     /locus_tag="WIE35_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ4154186.1"
                     /translation="MRLSDGIFDTKQVTKRVFDGKGEAKIPSYTWHHHQDTGRMQLIS
                     ERKHTQTGHIGGEAMDKGK"
     gene            2826..3224
                     /locus_tag="WIE35_001276"
     CDS             2826..3224
                     /locus_tag="WIE35_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154187.1"
                     /translation="MLGLTRNNCDDLGFAILCYERNAADKKEFLDWLYGIVRTVPAES
                     FPRYLIDLIDCLESDDCSINIYNIIGFVPTTGMTKKEKDAVYGITYIRNQEFYDIPIT
                     REKALKALKKHPEIYQKFRHFFPFVELPPL"
     gene            3325..3714
                     /locus_tag="WIE35_001277"
     CDS             3325..3714
                     /locus_tag="WIE35_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154188.1"
                     /translation="MWKIIKEDSNDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDVGIGDIGNIVGFAPSSSLPKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            3862..4251
                     /locus_tag="WIE35_001278"
     CDS             3862..4251
                     /locus_tag="WIE35_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154189.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
     gene            complement(4292..5749)
                     /locus_tag="WIE35_001279"
     CDS             complement(4292..5749)
                     /locus_tag="WIE35_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ4154190.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSYIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(5764..6594)
                     /locus_tag="WIE35_001280"
     CDS             complement(5764..6594)
                     /locus_tag="WIE35_001280"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ4154191.1"
                     /translation="MAHYAIGDIQGCFDELTSLLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(6672..>6945)
                     /locus_tag="WIE35_001281"
     CDS             complement(6672..>6945)
                     /locus_tag="WIE35_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ4154192.1"
                     /translation="TMTVVGNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTD
                     LGNFAVFNEVMAEFIAEPFPARAAVGVASLPKGVQVEAEAVLVLNA"
BASE COUNT         1983 a         1309 c         1865 g         1788 t
ORIGIN      
        1 ctcaaagact atgccgcagc agccatccgc gattgggcag tccaaaaccc caatgccgca
       61 caaggcatag aagccgtcag caatatcttt acggcagtca tccccatcaa agggattgga
      121 gctgttcggg gaaaatacgg cttgggcggc atcacggcac atcctgtcaa gcggtcgcag
      181 atgggcgaga tcgcattgcc gaaagggaaa tccgccgtca gcgacaattt tgccgatgcg
      241 gcatacgcca aatacccgtc cccttaccat tcccgaaata tccgttcaaa cttggagcag
      301 cgttacggca aagaaaacat cacctcctca accgtgccgc cgtcaaacgg aaagaatgtg
      361 aaactggcaa acaaacgcca cccgaagacc aaagtgccgt ttgacggtaa agggtttccg
      421 aattttgaga agcacgtgaa atatgatacg aagctcgata ttcaagaatt atcggggggc
      481 ggtataccta aggctaagcc tgtgtttgat gcgaaaccga gatgggaggt tgataggaag
      541 cttaataaat tgacaactcg tgagcaggtg gagaaaaatg ttcaggaaac gagaagaagg
      601 agtcagagta gtcagtttaa agcccatgcg caacgagaat gggaaaataa aacagggtta
      661 gattttaatc attttatagg tggtgatatc aataagaaag gcacagtaac aggagggcat
      721 agtctaaccc gtggtgatgt acgggtgata caacaaacct cggcacctga taaacatggg
      781 gtttatcaag cgacagtgga aattaaaaag cctgatggaa gttgggaaat gaaaaaagga
      841 aggggcggaa aacctatgac aaatcattct atgttcccaa aagattggga tgaggctaga
      901 attagggctg aagttacttc ggcttgggag aaaagaaagg aattagaagg tcaaaaatgg
      961 agaggtacaa gtaaatctgg tgttgaaata gaaggattta cggaacctaa tcgaacagct
     1021 tatccacttt ataatagtaa atgaagtatt gattaaaaag gtgtttttaa atgtataaaa
     1081 taaaatgttt ttatgatcag tttagttctg gtgaattatt taactactgc cagtttttag
     1141 ataattcttc taatcaaaag ataaatacac gaggaacggt gtatcaatat attatttatg
     1201 ttttaacagg agacctttat ctacaaaaag acatagatga aaatttagaa tttatccatc
     1261 aagcagaaaa taatcccaat gaagtttatt caggaggtgg acaaggtttt tgctgggata
     1321 tttctgctga aaaagtagtt ttctatcaca acgaatttga tgaagaagac ggctggcctg
     1381 atttaagctg ttcgctgcat acttttaaaa ctgctttaat tgcttggaat gcttttttgc
     1441 aattgcctaa aagtattcat tcggtggtgg agactgtgat tgaggaataa gcataattag
     1501 cttaatgaat agaatcagcg atatagattg gactgcaaat ccacgcttat acgctgtgcc
     1561 atgattaaca ggaagagagc aacttggatg ggctagtagg gatggtaagc atttaaatat
     1621 atcaattgat ggaaagatta cacacaaatg actaatttaa aattagattt ctactctgaa
     1681 gttattataa aagattcttg ccctaatgat ttgttagaaa atggtgagac tattaaagga
     1741 aaaaaaggag tggtattagg tataagcgaa gaagatggta taatctatgg ctatactatt
     1801 ttactttttg atataaaata ttgtatatat atagataaga aatatattat accgactgga
     1861 aaaaaattct ctcgcgatga tttttattag ctaatttaag taatttaagc aagtttttta
     1921 gatactgaaa ctggtaatta tgtttatgta agcataagga gttcagattg aaggattcta
     1981 cagggcaaat gaggctggca accaaggatt tggcggaagc cattaaacga ggagaagtac
     2041 gtagttctgc ttttacaaca aagcaactaa aggcaatcga aaaaggagaa tctaaaatac
     2101 caaattacac ttggcatcat catcaagaca ctggaaggat gcaattgatt aatgaagact
     2161 tgcatcatga taccggccat atcggttgga gagcgatgag taaaggaaag taactatgtg
     2221 gaaaatcata aaagaggata gtaatgattt aggatttgca attaaatgct tattctctca
     2281 gtctattgat ttaaatgaat tcaaattatg gattgaacaa gtaatacgag atatgcccat
     2341 cgaggacatc ccgttttata tttttgattt ggcggatttt gatgggggaa ttgccgatat
     2401 tgacaatatt gtaggttttg tttcgagttg cagcctatca aaatcgaaaa aaaatgcctt
     2461 gacgggcatt gccttcttaa ggggtataga tgtctatgac cctcccgttt caaaagaaaa
     2521 agcattaaaa gccttagaga aatatcctga aatttatcag aagtttcagc atttctttcc
     2581 gtttgtagag ctgcctccgc tttaaacagt caaaatgccg tctgaaacaa tattcggctt
     2641 tcagacggca tttttgatac aaagcaggta accaaaagag tgtttgacgg aaaaggagaa
     2701 gctaaaatac cgagctatac ttggcatcat catcaggata cgggacgaat gcagttaata
     2761 tccgaaagaa agcatacgca aacagggcat ataggtggtg aagcaatgga taaaggaaaa
     2821 tgattatgtt ggggcttaca cggaacaatt gtgatgattt gggatttgct atattgtgtt
     2881 atgagcgcaa tgcagccgac aaaaaagaat tcttggattg gttatatgga attgtcagga
     2941 ctgtccctgc cgaaagtttt ccaaggtatc tgattgattt aatagattgt ttggaatcag
     3001 atgattgttc cataaatata tataatatta ttggctttgt tccaactacc ggtatgacga
     3061 aaaaggaaaa agatgccgta tatggcatta cctatatacg gaatcaagag ttttacgata
     3121 ttccgattac tagggagaag gcgttaaaag ccttaaaaaa acatcctgaa atttatcaga
     3181 agtttcggca tttctttccg tttgtagaac tgcccccgct ttaaacagtc aaaatgccgt
     3241 ctgaaacgat attcggcttt cagacggtat ttttgatata aagcgggtaa ctaaaagagc
     3301 gtttgacggc aaaggaagat aattatgtgg aaaattataa aagaggatag taatgattta
     3361 ggattcgcaa ttaaatgctt attctctcaa tctattgatt taaatgaatt caagttatgg
     3421 attgaacaag taatacgaga tatgcccatt gaggacatcc ctttttatat ttttgatttg
     3481 gcggatttcg atgtaggaat tggcgatatt ggcaatattg taggttttgc tccgagttcc
     3541 agcctgccga aatcgaaaaa aaatgccttg accggcattg ccttcttaag ggggatagat
     3601 gtctatgacc ctcccgtttc aaaagaaaaa gcattaaaag ccttaaagaa acatcctgaa
     3661 atttatcaga aatttcagca tttctttccg tttgtagagc tgcctccgct ttaaacagtc
     3721 aaaatgccgt ctgaaagacc gtccgccgtt cagacggcat ttttgatata aagcggataa
     3781 ctaaaagagc gtttgacggc aaaggagaat ctaaaatacc ggatgtatcg gttgggaagc
     3841 aatggataaa ggtaaataat tatgtggaaa attagtaaag aaaattgtga agatttagga
     3901 tttgcaatag tctgtatgtt ctatgatgct attaatcttt ctgaatttaa attatggttg
     3961 gatatagttg tcagagatac tcctattgat acaattccat tgtatatttt tgatttgatt
     4021 gattttgata agagtatagg ggaaatttat gatgtaattg gagtcgttaa ttatggttac
     4081 atttcaaatg atcaaaaaaa tgcattaacg ggcattgcct tcttaagggg gatagatgtc
     4141 tatgatccgc ctatttcaaa agaaaaagca ttaaaagcct tagagaaata tcctgaaatt
     4201 tatcagaggt ttcagcattt ctttcctttt gtagagcttc cgctttttta aaagacaata
     4261 tgccgtctga aaagttttca gacggcattt tttatttctt ccagtaggcg ggggtgaaga
     4321 ggatgaagac ggtgaagatt tccagcctgc ccaagagcat ggcggtaacg cagatccatt
     4381 tctgcatcac gtccaaaccg gcgtaattgc cggcgggccc gacttcgccc aggccggggc
     4441 cggcgttggt gatgcaggcg atgacggcgg tgaaggcggt ggtaaattcc ataccgctcg
     4501 ccatcagcag gaagctgaag aggacgacgg tcataaagta gatgaagatg aaggacataa
     4561 cggtcagcgc gaggcggtcg ggtatggcct tgccgctgat tttgacggtg cggacggctt
     4621 tggggtgcag cagcaccatc atttcgcgca ggctgaattt gaacaggacg agggcgcgta
     4681 tggttttgat gccgccgccg gtcgagccgg agttggcgag gatgttggcg aggaaaaaca
     4741 tccacaggga aatcaggagc ggccattgtg cgaagtcggt gttggccagc ccgtttgcca
     4801 gtccgatgga gacgaagttg aaggcggtgt agcgcaggga ttcggtaaaa ccggcgtaat
     4861 agccggtgtg ccacaggtac agggcggcgg caaggatgct gccggagagc agcagcagca
     4921 tcgtccggca ttcttcgtct ttccaatagg ttttgaggct gcggctgttg agggcggcga
     4981 aatggttggc aaaattgatg ccgccgacaa tggtgaaaac gatgatgacc gcttcgatga
     5041 ggggggagtt gtaataagct atgctggcat cgtgggtgga aaacccgccc agcgagaggg
     5101 tcgccatcgc gtgacagacg gcatcgaacc agcccatccc ggcaaaatgc aggcaggctg
     5161 ccgcgaggat ggtgatcagg gtgtagccga accagagttt tttcgccact tgggaaatgc
     5221 gcggcgacat tttgctttct ttgtcaatgc cggggatttc ggctttgaat aattgcgtgc
     5281 cgcctacgcc gagcataggc aggatggcga cggcaaggac gatgatgccc atcccgccca
     5341 gccagttgag catatgccgc caaaagttga cggagggggc gagcccgtcg acgtggggga
     5401 tgacggtcgc gccggtggtg gtcagtcccg acatcgattc aaaaaatgcg tcggtaaagc
     5461 ccatattcgg gaaatacagg tacatcggca tcgcagccat agcggcaaac gccagccaca
     5521 acatcaggac gagggtgaag ccgtcgcgcg ggcgcagttc gcgcctgaac cggagggtgg
     5581 cgagccggac gatgtacgag ccggaaaggg taacggtcgc ggtggtggcg aaggcggtgt
     5641 acgcgccgtc cgaaaaggcg taggagaggg cggcgggtat cagcaggata aaggaaaaca
     5701 gcatacccag tcgggagagg acgtgggcga tgggcaggat tttgtgcata gtggggcggt
     5761 ccgttatttt gcgaagcttt tccagtctat gccgccggcg gcttggactt gggtaatttc
     5821 ttccgtttcg aggttgacgg cggtcagctg tccgccccac agcgcgccgg tgtccagcga
     5881 gatgacgttg tcggcattcg tgtagcccag cgaggaccag tgtccgaaga tgatggtgcg
     5941 gtcgaggttt tgccggtcgg gggctttgaa ccacgggcgc aggtaaggcg gcattttttt
     6001 cactgtggat ttgtagtcga aatccagttc gtttttaaag gtcagggcgc gcatccgcgt
     6061 gaaggcgttg acgatgaagc gcaggcgggc atagccttcc aaaccttcgt cccacgcggc
     6121 cggtttgttg ccgtacattt tggagaagaa tttgacgtat tttttgccgc gcagttcggc
     6181 ttcggcttct ctagcgagcg attcggcttt ggcgatgcgc cattgcggca ggatgccggc
     6241 gtgtaccatc acgcggctgc cctcgcgtat caacagcggt tgcgcacgca gccagtcgag
     6301 catttttttt ccgtcggggt gtttgagtat gggttcgatt gtgtcgctgc gtttgggcgc
     6361 gccttcgccg cagccgacag cgagcaggtg caggtcgtgg ttgccgagga cgatttgcac
     6421 gctgttttcg tgccggatgc agaattgcag cgtttcgagg gatttcgggc cgcggttgac
     6481 gatatcaccc gtcagccaga gggtgtccgt gccgtggttg aagccgattt tgccgagcag
     6541 tgaggtcagt tcgtcgaaac agccttgtat gtcgccgatt gcgtaatgtg ccattgcaga
     6601 tgttgtgaag tgggaaagtg ttgcggttcg gacggcatgg ttttgaaata tcatgcagtc
     6661 cgaacgtgga attatgcgtt caaaacgagg acggcttcgg cttcgacctg cacgcctttg
     6721 ggcagcgagg caacgccgac ggcggcgcgg gcggggaagg gctcggcgat aaattccgcc
     6781 atgacttcgt tgaagacggc aaaattgccc aagtcggtca ggtaggcgtt gagtttgacg
     6841 atgtcggcca gcgtgccgcc tgccgcttcg gcgacggctt gcaggttttg gaacacttgg
     6901 cgcgcttcgg cgcggaaatc gccgttgccg acgacggtca tcgtg
//
LOCUS       DAWHXZ010000099         6944 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000099 DAWHXZ010000000
VERSION     DAWHXZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.099"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            74..703
                     /locus_tag="WIE35_001282"
     CDS             74..703
                     /locus_tag="WIE35_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ4154193.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
     gene            849..2510
                     /locus_tag="WIE35_001283"
     CDS             849..2510
                     /locus_tag="WIE35_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ4154194.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPTAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGIVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVLLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENI"
     gene            2627..3232
                     /locus_tag="WIE35_001284"
     CDS             2627..3232
                     /locus_tag="WIE35_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ4154195.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            3304..3696
                     /locus_tag="WIE35_001285"
     CDS             3304..3696
                     /locus_tag="WIE35_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ4154196.1"
                     /translation="MPTSTKPYVLRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3725..3874)
                     /locus_tag="WIE35_001286"
     CDS             complement(3725..3874)
                     /locus_tag="WIE35_001286"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154197.1"
                     /translation="MPSEARIAGSDSIFNVPISNRQGQHGFNTPKQVLPINGFRLRPI
                     GQNAV"
     gene            complement(3895..4341)
                     /locus_tag="WIE35_001287"
     CDS             complement(3895..4341)
                     /locus_tag="WIE35_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ4154198.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSVKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(4473..4685)
                     /locus_tag="WIE35_001288"
     CDS             complement(4473..4685)
                     /locus_tag="WIE35_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ4154199.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(4974..6821)
                     /locus_tag="WIE35_001289"
     CDS             complement(4974..6821)
                     /locus_tag="WIE35_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ4154200.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTHAPSSKTLADNIETAGLSAS
                     VPTRPAEPEGKTLADYSSYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKSL
                     GARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLE
                     QAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGK
                     EARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQLT
                     DDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKLY
                     KQAVATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFQNSRT
                     AGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLR
                     YISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSHVGAQGLMQVMPATAREIA
                     GKIGMDAAQFYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTPL
                     EGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGIVPAR"
BASE COUNT         1374 a         1855 c         1904 g         1811 t
ORIGIN      
        1 tggggttgaa gttgacatct acaaagttta atgtttgaat acattaaaaa aatatatttg
       61 aatgagatgt tttatgattc aaaatgttgt tacttcaata atcctgtatt ctgggacagc
      121 cgtagactta cttattatcc taatgttatt ttttgccaaa agaaaaagca gaaaagacat
      181 cattaacatc tatttaggac aatttctagg ctctgttagc ctgatattgc taagtttgct
      241 ttttgcattt gtcttagatt atattcctag taaagagatt ttaggtttgc tcggcttgat
      301 tccaattttc ctaggcatca aagttttgct gttaggagat tctgatggag aggctattgc
      361 caaagagggt ttgcgcaaag ataataaaaa cctgattttt ctagtcgcta tgattacttt
      421 tgcaagttgt ggtgctgaca atattggtgt ctttgttcca tattttacta ccttaaattt
      481 agcgaatttg atagtggctt tacttacctt tctagtcatg atttatctct tggttttttc
      541 tgcccaaaaa ttagcacaag tcccttctgt tggagaaact ttggaaaaat atagcagatg
      601 gtttgttgct gttgtttatt tgggattggg ggtatatatc ctgattgaaa acaacagttt
      661 tgatatgcta tggactgtgt cgggccagga aaaaatatta tgaaaaagat agtatctgca
      721 aagactgcca tgtgcagtct tttttgttgc cggtcttttt gtgtctgatg ccgtctgaag
      781 cagtctctgc acgacctttg tgcgaatatt tgctacactt ggcaatccgt ccatttcttc
      841 tcacgcttat gctgacctac actccgcccg atgcccgccc gcccgccaaa acccacgaaa
      901 agccgtggct gctgctgttg atggcgtttg cctggttgtg gcccggcgtg ttttcccacg
      961 atttgtggaa tcctgccgaa cctgccgtct ataccgccgt cgaagcactg gcaggcagcc
     1021 ccaccccttt ggttgcccat ctgttcggtc aaaccgattt cggcataccg cccgtgtatc
     1081 tttgggttgc cgccgcgttc aaacatttgc tgtcgccgtg ggcagccgac ccgtatgatg
     1141 ccgcacgctt tgccggcgtg tttttcgccg ttgtcggact gacttcctgc ggctttgccg
     1201 gtttcaactt tttgggcaga caccacgggc gcagcgtcgt cctgattctc atcggctgta
     1261 tcgggctgat tccagttgcc catttcctca accccactgc cgccgccttt gccgccgccg
     1321 gactggtgct gcacggttat tctttggctc gccggcgcgt gattgccgcc tcttttctgc
     1381 tcggtacggg ctggacgctg atgtcgttgg cagcagctta tccggcagca tttgccctga
     1441 tgctgccctt gcccgtactg atgtttttcc gtccgtggca aagcaggcgt ttgatgttga
     1501 cggcagtcgc ctcacttgcc tttgccctgc cgcttatgac cgtttacccg ctgctcttgg
     1561 caaaaacgca gcccgcgctg ttcgcgcaat ggctcgacga tcacgttttc ggtacgttcg
     1621 gcggcgtgcg gcacgttcag acggcattca gtttgtttta ctatctgaaa aacctgcttt
     1681 ggtttgcatt gcctgcgctg ccgctggcgg tttggacggt ttgccgcacg cgcctgtttt
     1741 cgaccgactg ggggattttg ggcattgtgt ggatgcttgc cgttttggtg ctgcttgccg
     1801 tcaatccgca gcgttttcag gataacctcg tctggctgct tccgccgctt gccctgttcg
     1861 gcgcggcgca actggacagc ctgaggcgcg gcgcggcggc gtttgtcaac tggttcggca
     1921 ttatggcgtt cggactgttt gccgtgttcc tgtggacggg ctttttcgcc atgaattacg
     1981 gctggcccgc caagcttgcc gaacgcgccg cctacttcag cccgtattac gttcccgaca
     2041 tcgatcccat tccgatggcg gttgccgtac tgttcacacc cttgtggctg tgggcgatta
     2101 cccgcaaaaa catacgcggc aggcaggcgg ttaccaactg ggcggcaggc gttaccctga
     2161 cctgggcttt gctgatgacg ctgttcctgc cgtggctgga cgcggcgaaa agccacgcgc
     2221 cggtcgtccg gagtatggag gcatcgcttt ccccggaatt aaaacgggag ctttcagacg
     2281 gcatcgagtg tatcggcata ggcggcggcg acctgcacac gcggattgtt tggacgcagt
     2341 acggcacatt gccgcaccgc gtcggcgatg tacaatgccg ctaccgcatc gtcctcctgc
     2401 cccaaaatgc ggatgcgccg caaggctggc agacggtttg gcagggtgcg cgtccgcgca
     2461 acaaagacag taagttcgca ctgatacgga aaatcgggga aaatatataa aaaacaacag
     2521 attgagccga atttctggat taagtgccgg aaatcgcgta taattgcgcg attaaacctt
     2581 tataaatcag ccgttttaca ggcatcacac aggagcgaca attattatga tgaccctcta
     2641 ttccggcatt acctgcccct tcagccaccg ctgccgcttc gttttgtacg aaaaaggcat
     2701 ggattttgaa atcaaagacg tcgatattta caacaagccc gaagacctcg ccgtcatgaa
     2761 cccgtataac caagttcccg tgctggtcga gcgcgatttg gtgctgcacg agtccaacat
     2821 catcaacgaa tacatcgacg aacgcttccc ccatccgcag cttatgcccg gtgatcccgt
     2881 tatgcgcggc aggggccggc tggtgctgta ccgtatggaa aaagaattgt tcaaccacgt
     2941 ccaagttttg gaaaaccccg ccgccaccaa caaggaacag gcaaaagcgc gcgaagccat
     3001 cggcaacggt ctgaccatgc ttgccccttc gttcagcaaa agcaaataca tcctcggcga
     3061 agatttttct atgattgatg tcgcccttgc tccgctgctg tggcggctcg accactacga
     3121 tgtcaaactg ggcaaaagtg ccgcgccgct gctcaaatac gccgagcgca tcttccagcg
     3181 cgaagccttt atcgaagcac tgacacccgc cgaaaaagcc atgcgcaaat aagtccgaaa
     3241 tgcttgcaaa acccaccgtt ttgcaggcat tttcctattt tggcgtacaa cacggaaccc
     3301 atcatgccca cctcaaccaa accctacgtc ctccgcgctc tctgcgaatg gtgcagcgac
     3361 aacagcctca caccgcacat ccttgtttgg gtcaacgaac acacgcgcgt ccccatgcag
     3421 tatgtccgcg acaatgaaat tatgctcaac atcggtgcga ccgccacgca aaaccttcaa
     3481 atcgacaacg attggatcag cttttccgcc cgtttcggcg gacaggcgca cgacatatgg
     3541 atacctgtcg gacacgtcct cagccttttc gcacgggaga ccggcgaagg tatggggttt
     3601 gaattggagg agtaccgccc cgacacgccg cctgaaaaca cctctgccga aaccgcgccc
     3661 cgacccgcca aaaaaggctt gaaattggtc aaataaatct atgccgtctg aacggaatcg
     3721 tgtttcagac ggcattttgt ccgatggggc gcaaacggaa tccgtttatc ggcaaaacct
     3781 gtttcggcgt attgaaaccg tgttgcccct gccgattcga tattgggaca ttgaaaatgc
     3841 tgtctgaacc tgcgatacgg gcttcagacg gcattttgtc cgatattcgg gcaatcaggc
     3901 ggtcagcacg gctttcagga ttttgttgac ttcgcccata tcggctttgc cggcaagtcg
     3961 ggttttcaat acgcccatca ctttgcccat atccgccata cctgccgcgc cggtttcggc
     4021 aacggcggca tcgaccgcag tacggatttc gccggcggag agcatttgcg gcaggtagcg
     4081 gtgcagtacc tcgatttcgg cgttttcttt gtctgccaaa tcctgacggc cggcttcagt
     4141 gtagattttc acgctgtctt tgcgctgttt gaccattttg gtcaggatgg cggtgatttt
     4201 ggcatcgtcg gcttcggtgc gttcgtccac ttcaaactgt ttgacggcgg cgttgatgag
     4261 gcggatggtg ccgagggaaa cttggtcttt ggcgcgcatc gcggttttca tgtcttcggt
     4321 aaggcggatt ttcaggctca taatgttctc gctgggatgt cggatggaaa cggcgggttt
     4381 cgatgccgtc tgaaaagcaa aacacaccgc agagcaggtc ttgcggtgtg tcctcatatc
     4441 acagggctga cctgtaatct gtacttgaac gtttagtaca ttttgggcgg cagttgttgg
     4501 ctgcgcaggc gtttttgcag gcgttttacg gctgccgctt ttttgcgttt gcgttcggta
     4561 gtcggttttt cgtaggcttc gcgggcgcgc agctcggtca gcaggccggt tttttctacg
     4621 gcgcgtttga aacggcgcat agcgacttca aagggttcgt tttcttttac gcggattgca
     4681 ggcattttat ttcctttaat aaattctgtt gttgcatctg cccatcatat cggtggaaag
     4741 ggtagggcag acggtgttga aggtttaccg tatcgctgtg ccttgcggcg gcgggatacg
     4801 cgccgtgacg gaaacatcac ctcctaacgg gtcggcttgt gccgtgtcaa gctgcttgaa
     4861 aaagcaggaa aaacaatttt cggattgtct tatatttatg gttcgccgtc aatgccgttc
     4921 gggataaaaa tgccgtctga aagaccgggc gggtttcaga cggcatcggt acgtcagcgt
     4981 gcaggaacaa tgcccatacg ctgtttgagc gggatgtgcg gcgcgccgaa gagggcggcg
     5041 tagtaggcgg cattggtcat cacttttttg acatagtcgc gcgtttcgga aaacgggatg
     5101 gtttcggcat ataccgcgcc ttcgaggggc gtgtccgcct gccatcggcg cgccctgccg
     5161 ggaccggcgt tatagcctgc ggtggcgagg acttcgttgt tttgcaggcg gcgtttggtg
     5221 tccgccatat accacgtccc catacggata ttgccgtcgg cggtgtaaaa ttgtgcggca
     5281 tccataccga ttttgccggc gatttcgcgc gcggtggcag gcataacctg catcagcccc
     5341 tgcgcgccta cgtgggattg cgcgcctata acgaagcggc tttcctgacg aatcagaccg
     5401 taaacccaag ccggatcgac attaacattt tgcgcgtggc ggattaccgt gtctttaaac
     5461 ggcgaaatat agcgcaaggt gtagttgagt ttgcggtcgg tgcgttccgc gctgttgacc
     5521 gccatatcgt aaaaaccgtg gtcgaacgcg gtttgcgcgg cggtcagcag cttgtcttcg
     5581 tcaaagccgc gtgtggcaaa acgccattcc gcctgagcct gacggcgcat tttcgcatcg
     5641 ccggcggttc ggctgttttg gaacagtacc agcgcgcgct taatcgcgcc gtcttccgcc
     5701 atacggagga cgctgttttt gccggcatcg ggaacattgt tgcgcgtatc gattttgcgc
     5761 cccaactctt cccctgccag tacggcataa aaattcctgc ccgtcgctac cgcctgtttg
     5821 tagagttttt ccgcctcttg cgtgttgccc gttgcggcgc ggctgcgtgc cagccagtag
     5881 agccaggtcg ggcttttttg cagtttttcg ggcatatgcg agattacgga agccagctcg
     5941 tcccaacgtc gggcgcgcaa ggcggcgcgg gcgtaccact cgatttggtc gtcggtcagt
     6001 tggcggcggt cggcaacctt gccgtaatag tccaaggcgg caggcacatt gaggttttgc
     6061 gactgataat gccccaatac gccccacgcg aaactgcgtt gttcgaggct taaaccgctt
     6121 tccatttcgg acagcagggc ggcggcattc ggcgatttgc gtgcttcttt gccgatgacg
     6181 ttcaacaggg catattcgcg cgaaccttgt gtaccgccgt caaacgggct gcccaatgcg
     6241 gcggcaaggt tgcgtgcgtc tgtggtttgg cggccggcca gcagtccgcg cacgcgcctc
     6301 caggcgtcgt tgccgtccaa caagccggat gcggctgcct gttccaacag tttggtgcag
     6361 cccgaaggca gtttgcccgt atttttgacc agttcggcgg cacgcgtata gtcgttgcgg
     6421 ctcgaatcgg cgtagcattc gacttcttgg gcgcgccctt caggtttgag tttggcatat
     6481 tcctgtgcaa acagcgtcca ctgtctgcgt gcgcccaaag acttcagcca ctcgttgcgg
     6541 acattttccg ccatcgcgct gtcgccggcg ttttccaaat aggcggcgac ggcggcatcg
     6601 tttttctgtt tcactgcatc cagtgcggaa gggtagctgc tgtaatctgc cagcgttttt
     6661 ccttcgggtt cggcagggcg ggtgggaacg cttgccgaaa gaccggcagt ttctatattg
     6721 tctgccaggg tcttgctgct gggcgcgtgt gtcgaagaac acgcggcaag caccagggcc
     6781 gccagcagcg gcagggaatg cttcatagag ggtaggtaca tcggatttcc ttaagaatcg
     6841 gaaccctgaa cggtcagggt tggaaaagac aaaatgccgt ctgaacaggc gtttgcccga
     6901 attatatgcc gaaactgcac cgcctttgga atgtttccga cata
//
LOCUS       DAWHXZ010000100         6929 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000100 DAWHXZ010000000
VERSION     DAWHXZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.100"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..266
                     /locus_tag="WIE35_001290"
     CDS             <1..266
                     /locus_tag="WIE35_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154201.1"
                     /translation="CPIKPKPTNHAQQRKEEAKNDSHRSVGDSNRVVREGKQYLDSDT
                     GNHVYVKGDKVVILTPDGRQVTQFKNSKANTSKRVKNGKWTPK"
     gene            244..525
                     /locus_tag="WIE35_001291"
     CDS             244..525
                     /locus_tag="WIE35_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154202.1"
                     /translation="MGNGHQNNRSPLEKRIFYLEHSGQYLMICALSDYSQNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEEAISHHLEDFS"
     gene            904..2037
                     /locus_tag="WIE35_001292"
     CDS             904..2037
                     /locus_tag="WIE35_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154203.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NTNAFVAKLARLFRNADRAVVIVKESMRTEESLAGTVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYYIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGYGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMTRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     VREEKQGDRLPDFPLNLEDLDKEVRRYAEAAARRSGGRRDLSH"
     gene            complement(2124..2399)
                     /locus_tag="WIE35_001293"
     CDS             complement(2124..2399)
                     /locus_tag="WIE35_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154204.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDTNLNGLNLWMKKGVENLWDDTVGKKARLMCISVFWIVVFNL"
     gene            complement(2416..3189)
                     /locus_tag="WIE35_001294"
     CDS             complement(2416..3189)
                     /locus_tag="WIE35_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ4154205.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEQTSFNNPEPMTGFE
                     HTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPE
                     YEAYEDKRHIPENPYFHEFYYIKKGENPAIITHWNNRVNQAEEDNYSTSVGSCINGFT
                     VQYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            complement(3575..3850)
                     /locus_tag="WIE35_001295"
     CDS             complement(3575..3850)
                     /locus_tag="WIE35_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ4154206.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRKLWEQAEKMPSET"
     gene            complement(3847..5130)
                     /locus_tag="WIE35_001296"
     CDS             complement(3847..5130)
                     /locus_tag="WIE35_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ4154207.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFASGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWPEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGPFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(5286..6704)
                     /gene="glnA"
                     /locus_tag="WIE35_001297"
     CDS             complement(5286..6704)
                     /gene="glnA"
                     /locus_tag="WIE35_001297"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ4154208.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGETAYFGPEPEFFVFDGIEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPTVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
BASE COUNT         1872 a         1640 c         1674 g         1743 t
ORIGIN      
        1 cgtgtccgat caaaccaaaa ccaaccaatc atgcccaaca aagaaaagaa gaagctaaaa
       61 acgattctca tcgaagtgtg ggagattcca atcgtgtcgt tcgcgaagga aagcaatatt
      121 tagattccga cacaggaaac catgtttatg taaaaggaga taaagtggtt attctaactc
      181 ctgatggaag acaggtaact caatttaaga actcgaaagc caatacgtca aaaagggtaa
      241 aaaatgggaa atggacacca aaataatcga tctcccttag aaaaaagaat tttttatctg
      301 gagcattcgg ggcagtatct gatgatttgc gccctatcag attactccca aaataaacat
      361 actgtcgtta tggcgaattt tctctatcca aatgaaaaaa tggattggag aaatttagat
      421 gatttgttca atgagttggt tttagaggaa ttacagtctt catttatgga ttggtatccg
      481 actattgaag aggcaattag ccatcattta gaagactttt cgtaacaagc ataaattaaa
      541 ggttgtctga aactatgttc gattttcgac gacattttcc tcattggagg gggcggcaat
      601 ttatggaaaa tagatttgaa aaaatcgtta aggatatttt tagttttacc caatttgttc
      661 cgaagcagaa aatgagattc ccaccccatc ctcccgttat gacgactgtt ggtgggaatt
      721 ggaaatcttg aatgcctctg catttgaatt agtcaaaaaa ttgaatcaaa gcaaacaaga
      781 gtaacaagct gcagcctgca taggggtttt taaagtacgg cagcaaccgt agattaaatt
      841 aaaaatcaaa caatcaaagc tcgtctgata taatcgatat attctgttaa acgaaaaata
      901 aatatgttta aaaaattcaa accggtactg ttgtcatttt ttgcacttgt atttgccttt
      961 tggctgggga cgggtattgc ctatgagatt aatccgcgtt ggtttttgag cgatacggca
     1021 actgaaatac ctgaaaatac gaatgctttt gtggcgaaac ttgcccgcct gttccgaaat
     1081 gccgacaggg cggttgtcat cgtgaaggaa tcgatgagga cggaggaaag tcttgccgga
     1141 actgtggatg acggtccgtt gcagtcggag aaggattatc tcgcgctcgc tatccggctc
     1201 agtcgtttga aagaaaaggc gaaatggttt cacgtaacgg agcaggaaca tggggaagag
     1261 gtttggctgg attactatat cggcgagggc ggtttggttg cggtttcgct ttcgcaacgc
     1321 tcgccggaag cgtttgttaa tgccgaatat ctgtatcgga acgatcgtcc gttttctgta
     1381 aatgtgtacg gcggaacggc tcacggagaa aattatgaaa cgacaggaga atatcgggtt
     1441 gtttggcaac cggacggttc ggtatttgat gcgtcgggat acgggaaaat cggggaagat
     1501 gtttatgagc attgcctcgg gtgttatcag atggcccagg tatatttggc gaaataccgg
     1561 gatgtcgcga atgatgagca gaaggtttgg gacttccgcg aagagagcaa ccggattgcg
     1621 tcggactcgc gcgattctgt gttttatcag aatatgcggg aattgatgac ccgggggatg
     1681 aaggcgaaca gtcttgtggt cggctatgat gcggacggtc tgccgcaaaa agtctattgg
     1741 agtttcgaca atgggaaaaa acgccagagt ttcgaatatt atttgaaaaa cggaaatctt
     1801 tttattgcac aatcttcgac ggtagcattg aaagcggatg gcgtaacggc ggatatacag
     1861 acctatcatg cgcaacagac gtggtatttg gatggcgggc ggattgtccg cgaagagaaa
     1921 cagggagaca gactgcctga ttttcctttg aacttggaag atttggacaa agaagtgcgc
     1981 cgttatgcgg aggctgcggc gagacgttcg ggcggcaggc gcgacctttc tcactgaacg
     2041 caaatgccgt ctgaaaccgg ttttcagacg gcattggaga gtcaaacgct ttttgcaata
     2101 ccgtatatgc cgaatccgat tcgctacaaa ttgaaaacca caatccaaaa aacggaaata
     2161 cacattaaac gggccttttt accgaccgta tcatcccata ggttttccac accttttttc
     2221 atccataaat tcaaaccatt taagtttgta tccttaccga caattgcagc agcatcatcc
     2281 gcataacggt cacccaaatt agccaaataa cgccaaccgc cataggtatc gccacggaaa
     2341 gcataaccac gcaaggtttg gatttgttct ggtgttaaaa caacttcacc ctcattcata
     2401 ataagatcct ttaaattatt ttttacttga attgtttaca aaactctgta ccaattgctc
     2461 tacttgttgg tgataaccta ccaactcctg ctgtgtgagc tgctgctttt cccgaataaa
     2521 cgggtaatac tgtaccgtga aaccgttaat acaggaacct acgctagtgc tataattatc
     2581 ttcttcagcc tggtttactc gattattcca atgagtaata atcgccggat tttctccttt
     2641 tttaatatag taaaattcat gaaaatatgg attttcagga atatgtcttt tatcttcata
     2701 agcttcatat tcaggatagg cagatggcat ttttttaacc ccgtaagtaa gcaatctttc
     2761 tcccatagtc gtacttaaac tacacgcatc ttttcggata acaatatcgt ccacgctatc
     2821 caaaccatcg cgtagaaatt ggataaagtg tgctttgttt tgctggttgt atggctcaag
     2881 aacccagcct tggtcggttt ttttgtaata gacgctaatc tctatcaaat taatggagta
     2941 agcatcctgc ccgggcgtgt cggaaagcca ttttgtggca ttgtcttgcg tataccgtgc
     3001 aagatagcca taagggataa ccattttggt gccctgaaaa tcaaatgtaa ccgtatgttc
     3061 aaatcctgtc attggctcgg gattgttgaa actggtttgt tcattaacag gttcacatga
     3121 ggtcatagta aaaacactcc caataaacaa aataaaaagt tgataaatgg tagtagcata
     3181 tggtctcata atttcaagcc tagaaattag ttaaagaata tattatggta gatactaact
     3241 ctttaagttg acatattgtg cgatgtcgag ggctgtacca tattttccca tcgtattttc
     3301 ccgttcttta tatgagcggc aggcatcctc ataaaagaag ggataccgat acggaaaaat
     3361 accccggcat aaaaaatata ctgattaatc aatacaaatc caacaaacag ttcatcgtat
     3421 tcttttctgt tggcgtataa aaaatactga tatatgcctt cactttcata gggcgacgct
     3481 ttatgtaccg tcctgtgtgt tgaaacgtcg gttttcctta ccgcctgccc cgcttttccc
     3541 tgattgctgc caactgccgt ccgaacctga accgtcaggt ttcagacggc attttttccg
     3601 cctgttccca caatttccgt ttttgtgcgg cggtcagttt tttgacggcg atttcctgtt
     3661 tgagcgccgt gcctttatcc atcccgcctg caacgatacg catcgccacc ggtttgaata
     3721 cgcgggtata tttcgcgcct ttaccggttg tgtgggcggc aagccgctgt tgcggattcg
     3781 ggctgatgcc gcaatagaac gcgctgtttt cacacaatat caggtaaacg ctccagtttg
     3841 acgcgttcat ctgcctgcat cctgagtttt ttccccgttc caaggcgcaa ctttcagcag
     3901 cagcagcata cccggcaggg caagtatgaa acacagctgg aaaaacggta cataaccgag
     3961 ccattcgatc agataaccgg caaaggaatt gatgaccgtg cgcgggacgg cggacaggct
     4021 ggtaaacagc gcaagctgcg ttgcggtaaa tgcgggattg gtttcacgcg ccatatacga
     4081 tacgaacgcc gccgtcccca accccacgcc gaccgcttcc gcgccgataa ctgccgccag
     4141 catcagcctc tcgcctgtgc cgaccgtgtc gaaaggtccg aaccctgcca gccatacaaa
     4201 ccccaaaacg gttacagcct gcaccgcgcc gaatagccac aaggctttgt ttacgccgat
     4261 tttcagcatc cacacgccgc ccaagatacc tgccgccact gccggccaca gtcctgcatt
     4321 ttttgcaatc aaaccgatgt ctgtcttact gaaacccata tccagataaa acggcgttgc
     4381 caacgcggtt gccatactgt cgccgagttt gtaaaggaag ataaacagca gcacgcacac
     4441 cgccgaagcg atgcccttgc gcgtaaaaaa ttctttaaac ggctctacca cggtctgctt
     4501 caacgtttta ggaacggcag gcggcaacac gggttcgcgc gcaagaaaca gcgtcatcag
     4561 aagaccgggc agcataaaca gcgaagtgat gacaaacacc tccggccacg gcatcctgtc
     4621 tgccagcacc aaactcaatg aaccgggaat cagggcggca acccgatagg cgttcacatg
     4681 aaccgagttg cccaaaccca attcctcatc cgacaaaatc tcgcgcctga acgcatccaa
     4741 tacaatgtcc tgactggcgg aaaaaaaagc gacaagcacc gacaagccgg caatcagcgg
     4801 cagatgatta cgagggttta aaaaggcata agccgccaaa gccgccagca aacctgcctg
     4861 cgtcagcagc atccacccgc gccgccgccc caaaacgggc agcctgaccg cgtccatcag
     4921 cggcgaccac aaaaatttcc aagtaaacgg cagaccgatt aacgccatca gcccgatgct
     4981 cttcaaatcc acctgctcgc tgcgtaacca cgccggaatc aggttaatca gaaagtacag
     5041 cggcagcccc gaggcaaacc ccgtaaaaat acagacaagc atattgcggg aaaagatttg
     5101 cccgataaaa cctgatttcg atgcagtcat cgttcgtgca gcccaaaaaa aacaaaagcc
     5161 tattgtagca aatatcgccc gcctgctgaa aaacgccgcc ccgcgccgcc ggtttgccgt
     5221 tcaaatgcaa aatgccgtct gaaactgtgt tcagacggca ttgcttttct gaaaaccaga
     5281 cgtgcttaca ggctgtaata catttcaaat tccagcgggt gcggcgccat gcggatgcgg
     5341 cgtacgtctt cttctttgaa agcgatgtag ctgtcgatcc agtctttgct gaacacgccg
     5401 ccgcgcagga ggaattcgtg gtcggctttg agggcggcca gtgcttcttc caaagaagcg
     5461 caaacggtcg gcaccaatgc atcttcttcc ggcggcagat cgtacaggtt tttatcggca
     5521 gggtcgcccg gatggatttt gttttgaatg ccgtccaaac ccgccatcaa cagggcggca
     5581 aatgccaaat acgggttggc ggtcggatcg gggaaacgcg cttcgatgcg gcgcgccttg
     5641 ctgctgttca cggacggaat gcggatggaa gcggaacggt ttttggcgga gtatgccagt
     5701 ttggtcggcg cttcaaagtg cggcacgagg cgtttgtagg agttggtgga cggattggta
     5761 atcgcgttca aggctttggc gtgtttgatg atgccgccga tgtagtagag cgcggtgtcg
     5821 ctcaagccgg catagccgtc gcctgcgaac aggttttgac cgtctttcca aatggattgg
     5881 tgaacgtgca taccgctgcc gttgtcgccc ataatgggtt tgggcatgaa agtggcggtt
     5941 ttgccgaagt tgtgggcaac gttttgaatc acatatttca tgtcttgggt ttggtcggcg
     6001 cgtttgacca aagtagcaaa gcgcgtgccg atttccattt ggctgccggt gccgacttcg
     6061 ctgtggtgca cttccacttc aataccgagt tcttccaaaa tgtttaccat cgccgaacgc
     6121 aaatcctgac cgcagtcaat cggtgcaaca ggtgcgtaac cgcctttgac ggtcgggcgg
     6181 tggccggtgt tttgaccgtc catatgcaga ccgcttgccc acgcgccgct ttcggacgtg
     6241 atttcgtaac gggttttgtg catatcggtt tcaaattcta tgccgtcgaa tacgaaaaac
     6301 tcgggttcgg gaccgaaata ggcggtctcg ccgataccgg aagatttcaa ataggcttcg
     6361 gctcggcggg cgatggagcg cgggtcgcgg tcgtaaccct gaccgtcggc gggatcgata
     6421 acgtcgcaag tcaacacaac agtcgcatca tcataaaaag gatcgacgaa ggctgtagac
     6481 gcatcgggac gcaactgcat atcggaagcc tgaatgcctt tccagccgcc gatagacgaa
     6541 ccgtcaaacg cctgaccgtt ttcgaaccac tcttcggggt cttccaacac gatgcgcgca
     6601 ggcacggtaa agtggtgctg cttgcctttg gtatcggtaa agcgcaaatc gacaaagcgg
     6661 gcttcgcttt cttcaatcaa ttttacggcg tttttaatag acatttttca gctcctggaa
     6721 aagggaaata gcaaaatata cggaacgcgg cagattagcc caaagccagc atgattctac
     6781 cacgtccatg ccgtctgaaa ctcgccctgc gtcaaatttc aagctggaaa atatagtttc
     6841 agacggcaag attgtcaaca atatgcttcg gtttgtctgt tcaaaagcag aatacccgcc
     6901 cttttgtccg aatgccgaca aaacagatg
//
LOCUS       DAWHXZ010000101         6879 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000101 DAWHXZ010000000
VERSION     DAWHXZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.101"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            108..1277
                     /gene="prpF"
                     /locus_tag="WIE35_001298"
     CDS             108..1277
                     /gene="prpF"
                     /locus_tag="WIE35_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ4154209.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1691..2890
                     /locus_tag="WIE35_001299"
     CDS             1691..2890
                     /locus_tag="WIE35_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ4154210.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSIVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMDKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELVGIL"
     gene            3248..3868
                     /locus_tag="WIE35_001300"
     CDS             3248..3868
                     /locus_tag="WIE35_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ4154211.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIVNQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(3995..4438)
                     /locus_tag="WIE35_001301"
     CDS             complement(3995..4438)
                     /locus_tag="WIE35_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ4154212.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4612..4980
                     /locus_tag="WIE35_001302"
     CDS             4612..4980
                     /locus_tag="WIE35_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ4154213.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFA
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            5025..6179
                     /locus_tag="WIE35_001303"
     CDS             5025..6179
                     /locus_tag="WIE35_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ4154214.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(6236..>6879)
                     /locus_tag="WIE35_001304"
     CDS             complement(6236..>6879)
                     /locus_tag="WIE35_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ4154215.1"
                     /translation="VPAILTALRPVLPEHTWISDVGSTKSSVIEAFRRCLPDRLHRCI
                     AAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGIALIENLWRAVGAEIYTMDA
                     QRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAATGFRDFTRIASGHPAVWAD
                     ICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYFEEAKTTRDRWLDGN"
BASE COUNT         1503 a         1899 c         1952 g         1525 t
ORIGIN      
        1 ggcgaacccc gacagtttgc ggaatcaaac ggctttggtc ggagtggcag cctaatccac
       61 tataaaaatc gtgggcagag cccacgctac ataaggagaa tctagaaatg ccgcaaatta
      121 aaattcccgc cgtttactac cgtggcggta catcaaaagg cgtgtttttc aaacgttccg
      181 acctgcccga ggcggcgcgg gaagcgggaa gcgcacgcga caaaatcctc ttgcgcgtac
      241 tcggcagccc ggatccctac ggcaagcaga tagacggttt gggcaacgcc agctcgtcca
      301 ccagcaaggc ggtgattttg gacaagtccg aacgcgccga tcacgatgtc gattaccttt
      361 tcgggcaagt ttccatcgac aaaccttttg tcgattggag tggcaactgc ggcaacctca
      421 ccgccgccgt gggcgcattt gccatcgagc aaggcttggt cgataaaggc aagattcctt
      481 cagacggcat ctgcacagtc aaaatctggc agaaaaacat cggcaaaacc attattgccc
      541 atgtaccgat gcaaaacggc gcagttttgg aaacaggcga ttttgagctc gacggcgtaa
      601 cgttcccggc agccgaagta caaatcgaat ttcttgatcc agccgacggc gaaggcagta
      661 tgttcccaac cggcaatttg gtcgatgaaa ttgatgtgcc gaatataggc cgtttgaaag
      721 ccacgctcat caacgcgggc attccgaccg ttttcctgaa tgccgccgac ttgggctaca
      781 cgggcaaaga gttgcaagac gacatcaaca acgatgccgc agctttggaa aaattcgaga
      841 aaatccgcgc ttacggtgcg ctgaaaatgg gtctaatcag cgacgtatcc gaagctgccg
      901 cccgcgcgca cacgccgaaa gtcgccttcg tcgcgcccgc cgccgattac accgcctcca
      961 gtggcaaaac cgtgaatgcc gccgacatcg atttgctggt acgcgccctg agcatgggca
     1021 aattgcacca cgcgatgatg ggtaccgcct ctgttgccat tgcgaccgcc gccgccgtgc
     1081 ccggtacgct ggtcaacctt gccgcaggcg gcggaacgcg taaagaagtg cgcttcgggc
     1141 atccttccgg cacattgcgc gtcggtgcag ccgccgaatg tcaggacgga caatggacgg
     1201 ccaccaaagc ggttatgagc cgcagcgcac gcgtgatgat ggaaggttgg gtcagggtgc
     1261 cggaagattg tttttaaatt gacgtagcat gggtttgccc gcgagccata aacaggtcgt
     1321 ctgaaaaaca agtaaacatc aaatcactga ccattccttt cccttgccct gtggcggaag
     1381 gcggcaaatc acaaggaaga acacggaaac cccgataaaa gacagcttcc cgtattaccg
     1441 tcattcccgc gcaggcggga atctagacct gtcaatatgg aggattggta ggggaaaaca
     1501 ggtttcgtga gttctacatt ctggattccc gccacagcct gtcctcgcgt aggcggggac
     1561 ggaataacga tagaaaatgc ggcatacgct ttgcccaaag aggccgtctg aaacaccttg
     1621 cgcctgatgt ctgccttttt cagacgaccc cacaccaaaa aaacaaccac aaactacaag
     1681 gagaaacatc atgtccgacc aactcatcct cgttctgaac tgcggcagtt catcgctcaa
     1741 aggcgccgtt atcgaccgca aaagcggcag catcgtccta agctgcctcg gcgaacgcct
     1801 gaccacgccc gaagccgtca ttacgttcag caaagacggc aacaaacgcc aagttcccct
     1861 gagcggccga aattgccacg ccggcgcggt gggtatgctt ttgaacgaac tggaaaaaca
     1921 cggtctgcac gaccgcatca aagccatcgg ccaccgcatc gcccacggcg gcgaaaaata
     1981 cagcgagtct gttttgatcg accaggccgt aatggacgaa ctcaatgcct gcattccgct
     2041 tgcgccgctg cacaaccccg ccaacatcag cggcatcctc gccgcgcagg aacatttccc
     2101 cggtctgccc aatgtcggcg tgatggatac ttcgttccac caaaccatgc cggagcgtgc
     2161 ctacacttat gccgtgccgc gcgagttgcg taaaaaatac gctttccgcc gctacggttt
     2221 ccacggcacc agtatgcgtt acgttgcccc tgaagccgca cgcatcttgg gcaaacctct
     2281 ggaagacatc cgcatgatta ttgcccactt aggcaacggc gcatccatta ccgccatcaa
     2341 aaacggcaaa tccgtcgata ccagtatggg tttcacgccg atcgaaggtt tggtaatggg
     2401 tacacgttgc ggcgacatcg atccgggcgt atacagctat ctgacttccc acgccgggat
     2461 ggatgttgcc caagtggatg aaatgctgaa caaaaaatca ggtttgctcg gtatttccga
     2521 actttccaac gactgccgca ccctcgaaat cgccgccgac gaaggccacg aaggcgcgcg
     2581 cctcgccctc gaagtcatga cctaccgcct cgccaaatac atcgcttcga tggctgtggc
     2641 ctgcggcggc gttgacgcac tcgtgttcac cggcggtatt ggcgaaaact cacgtaatat
     2701 ccgagctaaa accgtttcct atcttgattt cttgggtctg cacatcgaca ctaaagccaa
     2761 tatggacaaa cgctacggca attcgggtat tatcagcccg accgattctt ctccggctgt
     2821 tttggttgtc ccgaccaatg aagaactgat gattgcctgc gacactgccg aacttgtcgg
     2881 catcttgtag ccaaaaaagg gacgagtccg cacaatgccg tctgaaaccc caaacgcccg
     2941 attaggctga tgaggatttt agacggcatt gttcattttt ttgttatctt gcatttttgt
     3001 gcggacggtg gaatttcatc ctgtaaacat aaatatttgt cggaaaacag aaaccctccg
     3061 ccgccatttc tacgaaagca ggaaaccagc aacgcaaagc gacagggatt tgttggaaat
     3121 gaccgaaacc gaacgaaccg gattcccgcc tgcgcgggaa tgacgggatt ttctgttttt
     3181 gtggaaatga cgggattttg aatttcgggc gtacaatacg gaaaacatga cgataaggac
     3241 acaaaccatg gcacagtttt tcgccgttca tcccgacaat ccccaagaac gcctcatcaa
     3301 gcaggcggtt gaaatcgtca atcaaggcgg cgtggtggtt tatccgaccg attcgtgtta
     3361 cgccttgggt tgcaaactcg gcgataaggc ggcgatggaa cgcatactct ccatccgcaa
     3421 aatcgatttg aaacaccacc tgaccctgat gtgcgccgat ttgagcgagt tgggcacgta
     3481 cgccaaaatt gacaacgtac agtttcgtca gcttaaagcc gccacgcccg ggccttatac
     3541 ctttatcttg caggcgacga aggaagtgcc ggcgcgcacg ctgcacccga aacgcaaaac
     3601 catcgggctg cgtattcccg ataatgccat tgcacaagcc ctgctggggg aattgggcga
     3661 gccgctttta agctgcaccc tgatgctgcc cgaagacggc gaaccattga ccgatcctta
     3721 tgaaatccgc gagcgtttgg aacacgccgt cgatttggtg attgacggcg gctggtgcgg
     3781 aaccgagccg accaccgtcg tcgatatgac cgacggcacg gaattggtgc gccaaggttg
     3841 cggcgatacg gcggtgttcg gtttgtaggg aaaccgatgc cgtctgaagc atcggctgtt
     3901 cagacggcat tgcgcgcctt gccggcggca gtccgaaatg ccggcgcgta tcgcgctcgg
     3961 tcggaatatc cgtttgaaac ggcattttga tgcattactg caccgcaatc ggaattctcg
     4021 gttcgtagag caggtcgtag gtcggcttgt tgagcaggtc ttggagcgtg aaaccgtcca
     4081 gatacgtgaa aaatgacttc atcgcgccgc cgagtatgcc cgtcagccgg caggacggtg
     4141 taatcaggca ttcgttgttc tcgcccatgc actcgaccag ctgcatcggt tcgaggtggc
     4201 ggacaaccga gccgatgttg atgcggtcgg gcggtgcggc aagccgcaga ccgccgcctt
     4261 ttccgcgcac actgtggagg aagccgcctt tgaccagcgc ggtaacgacc ttcatcagat
     4321 ggcttttgga aatgccgtag gttacggcga tggtactgat gttgaccagc gcatcgtcgt
     4381 tgatggcagt gtagataagg acgcgcagcc cgtagtccgt atgttgtgtc aaatacatga
     4441 ttttctcggt atggatcgtt attcttatcg gtacggttta agtttcacgg aaaatacctt
     4501 aatggttgaa accctgtccg tcggggcggt agaatgcagc ctgtctgcgg cggtatgccg
     4561 tctgaaacat ccgcgctacc gtttgataat ttgttattgt aactcaaaat catgaaaccg
     4621 ttgaaacgac atcccgccct tatcgggctt tcgcgtgacc accaccattc gctttccctg
     4681 tgcgtgcgtc tgttgcggac gccggaagaa aggcatcggg acgaactcga accgcatttt
     4741 gccgaattgg aaacccattt tcgcgaagaa gaagccaagt ttgccccaat ttggcagaat
     4801 gtcgcccccg aattgaaaca acgtttcgag aaagaccacg cccgactgcg gcagatgatg
     4861 gcaagccccg aatacggtaa cgcggcgtgg aataccgctt ttgccacaac cctgcgcgac
     4921 cacgcgcgct ttgaagaacg cgagctgttt cccgccgccg aaccgttttt gccggcatga
     4981 ttccgttttg cggcaaatat attaatgata aacaaggaac acacatgaaa tttaccaagc
     5041 accccgtctg ggcaatggcg ttccgcccgt tttattcgct ggcggctctg tacggcgcat
     5101 tgtccgtatt gctgtggggt ttcggctaca cgggaacgca cgagctgtcc ggtttctatt
     5161 ggcacgcgca tgagatgatt tggggttatg ccggactggt cgtcgtcgcc ttcctgctga
     5221 ccgccgtcgc cacttggacg gggcagccgc ccacgcgggg cggcgttctg gtcggcttga
     5281 ctatcttttg gctggctgcg cggattgccg cctttatccc gggttggggt gcgtcggcaa
     5341 gcggcatact cggtacgctg tttttctggt acggcgcggt gtgcatggct ttgcccgtta
     5401 tccgttcaca gaatcaacgc aactatgttg ccgtgttcgc gctgttcgtc ttgggcggca
     5461 cgcatgcggc gttccacgtc cagctgcaca acggcaacct aggcggactc ttgagcggat
     5521 tacagtcggg cttggtgatg gtgtcgggtt ttatcggtct gattggtacg cggattattt
     5581 cgttttttac gtccaaacgg ttgaatgtgc cgcagattcc cagtccgaaa tgggtggcgc
     5641 aggcttcgct gtggctgccc atgctgaccg ccatgctgat ggcgcacggc gtgatgcctt
     5701 ggctgtcggc ggctttcgcg tttgcggcag gcgtgatctt taccgtgcag gtgtaccgct
     5761 ggtggtataa acccgtgttg aaagagccga tgctgtggat tctgtttgcc ggctatctgt
     5821 ttaccggatt ggggctgatt gcggtcggcg cgtcttattt caaacccgct ttcctcaatc
     5881 tgggtgtgca tctgatcggg gtcggcggta tcggcgtgct gactttgggc atgatggcgc
     5941 gtaccgcgct cggtcatacg ggcaatccga tttatccgcc gcccaaagcc gttcccgttg
     6001 cgttttggct gatgatggcg gcaaccgccg tccgtatggt tgccgtattt tcttccggca
     6061 ctgcctacac gcacagcata cgcacctctt cggttttgtt tgcactcgcg cttttggtgt
     6121 atgcgtggaa gtatattcct tggctgattc gtccgcgttc ggacggcagg cccggttgag
     6181 acaaaccgcc gcagatttcg ggtctgggcg gtttgctttt cagacggcag ggcggtcagt
     6241 tgccgtccag ccagcggtcg cgtgtggttt tggcttcttc aaaatagcgg tacagggctt
     6301 cgcggtcgtc ggtggtcagg atgtttgcca aaacgtccaa ctgtttgccc aagccttgaa
     6361 ccagttgcag caggctgtct ttgttggcaa ggcagatgtc cgcccacacg gcgggatgac
     6421 cggaggcgat gcgggtgaag tcccgaaagc ccgtggcggc gaatttcaga tattcctgtc
     6481 cgtcggggtg gtcgagaatc tggtggacat aggcgaaggc ggtcaggtgg ggcatatggg
     6541 agacggcggc gaaaaccgcg tcgtggcgtt gcgcgtccat cgtataaatt tccgccccga
     6601 ccgcgcgcca caggttttct atcaaggcaa tgccgtctga atgttcgccg ccgtgtggcg
     6661 tgatgatgag ttttctgtgg cggaacagcc cgaactgcgc ggcttgcgca ccgcttctgt
     6721 ccgaaccggc aatcgggtgg gcggcgatgc agcggtgcag gcggtcgggc agacagcggc
     6781 ggaaggcttc gatgaccgaa gatttggtgc tgccgacatc ggaaatccaa gtgtgttccg
     6841 gcaaaacggg gcgcagcgcg gtcaaaatgg cgggaacgg
//
LOCUS       DAWHXZ010000102         6851 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000102 DAWHXZ010000000
VERSION     DAWHXZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.102"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(23..2314)
                     /gene="clpA"
                     /locus_tag="WIE35_001305"
     CDS             complement(23..2314)
                     /gene="clpA"
                     /locus_tag="WIE35_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ4154216.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYSSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIILKVVDKFLLQLEHQLLDKK
                     VEAEFTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(2316..2618)
                     /gene="clpS"
                     /locus_tag="WIE35_001306"
     CDS             complement(2316..2618)
                     /gene="clpS"
                     /locus_tag="WIE35_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ4154217.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            2861..3064
                     /locus_tag="WIE35_001307"
     CDS             2861..3064
                     /locus_tag="WIE35_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ4154218.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            complement(3158..3349)
                     /locus_tag="WIE35_001308"
     CDS             complement(3158..3349)
                     /locus_tag="WIE35_001308"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154219.1"
                     /translation="MPSEDEGSTFCPVPARLPFYAAPPFADNIAHFFISTDFPLSVKI
                     LSHLSTTGYTQVQDKACGD"
     gene            complement(3361..4692)
                     /gene="pmbA"
                     /locus_tag="WIE35_001309"
     CDS             complement(3361..4692)
                     /gene="pmbA"
                     /locus_tag="WIE35_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ4154220.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            4846..5352
                     /locus_tag="WIE35_001310"
     CDS             4846..5352
                     /locus_tag="WIE35_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ4154221.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAIVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RTRMELLADDGALTQFMSDFPNADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MENGDAEI"
     gene            5399..6115
                     /locus_tag="WIE35_001311"
     CDS             5399..6115
                     /locus_tag="WIE35_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154222.1"
                     /translation="MLFRKTTAAVLAAALMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPVDIYYTVTEKHTDKSKLFGNILYTPPLLILDAA
                     GAVLALPAAALGAVVDAARK"
     gene            6184..>6851
                     /locus_tag="WIE35_001312"
     CDS             6184..>6851
                     /locus_tag="WIE35_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEZ4154223.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQTLTNCEAAAGRLADAVERKEKILIVADYDADGATACSVGMSGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGIKEP
                     NLGEL"
BASE COUNT         1445 a         1722 c         2022 g         1662 t
ORIGIN      
        1 ctttcagacg gcatttttga ttttatgcgg aggcggtttt tattttgacc ttgcttttct
       61 taaacttcaa cacggcttct tcttttgccg catcccagtc tatccgcacg aagccgccgt
      121 cggatagttt gccgaacagg agttcgtcgg cgagcggttt gcggattttt tcctgaatca
      181 ggcggtgcat cgggcgcgcg cccatttgcg ggtcgaaacc tttttccgcc agatatttgt
      241 gcaatgccga cgtgaattcg gcttcgactt ttttgtcgag gagctggtgt tcgagctgga
      301 gcaggaattt gtccacgact ttgaggatga tgggttcgga caagggtgca aacgggataa
      361 tcgcgtccaa gcggttgcgg aactcgggcg tgaagagctt gttgattgcc tgcatttcgt
      421 cgccgcgttc gcgcttggcg gtaaagccga ggctggggcg gctgaggctt tccgcgcctg
      481 cgttggtggt cataatcagg atgacgttgc ggaaatcggc actcttgccg ttgttgtcgg
      541 tcagtttgcc tgcgtccatg acttgcagga ggacgttgaa aatgtcgggg tgggcttttt
      601 cgatttcgtc caagagcaat acgcaatgcg gctgtttgtt gacggcttcg gtcaaaaggc
      661 cgccttgttc aaagccgacg tagcccggtg gcgcgccgat gaggcgcgat acggcatggc
      721 gttccatgta ttcggacata tcaaagcgtt gcagcggtac gcccagcgag taggcaagct
      781 gtttggcggc ttcggttttg cccacgccgg tcgggccgga gaagaggaag ctgcctatcg
      841 gtttgtcggg cagggcaagg ccggaacgcg acattttgac ggcggcaacc agcgcgtcga
      901 tggcgttttc ttgaccgtaa accatgtttt tcaaatcgcg accgaggaat tgcagcactt
      961 gtttgtcgtc gtgcgacacg gttttttcgg gaatccgcgc aactttggcg atgacggttt
     1021 caatttgtgc tttgccgatg acttttttct gtttggattt gggcagaatc cgttgcgccg
     1081 cgcctgcttc gtccatcacg tcgatggctt tgtcgggcag gaaacgctcg ttgatgtagc
     1141 gtgcggagag ttcggcggcg gcttcgagtg cgccttgcgt atagcggact tggtggaagg
     1201 cttcaaacat cggcttcaag ccgcgcagga tttgtacggt ttcggaaacg gtgggttcga
     1261 ccacgtcgat tttttggaag cggcggctta aggcgtggtc tttgtcgaaa atggtgcggt
     1321 attcgtcgta agtggtcgcg ccgatgcagc gcagcgaacc ttttgccagc gcgggtttga
     1381 gcaggttgga cgcgtccatg gtgccgccgc tggtgctgcc tgcgccgatg atggtgtgga
     1441 tttcgtcgat aaacaaaatg gcgtgcggga ttttttcgag ctgtttcaag acggatttga
     1501 cccgcgcttc aaagtcgccg cggtatttcg tgcccgccaa cagcgagccc atatccagcg
     1561 cgtacacttc ggcatctttg agcgcgtctg gaatgccgcc gttgacgatt tgatgtgcca
     1621 aaccttccgc cagcgcggtt ttgcccacgc ccgcttcgcc gaccaaaagc ggattgtttt
     1681 tgcggcggcg gcataggatt tgcaccagcc gttccatttc gtgtttgcga ccaatcaaag
     1741 ggtcgatacg gccggctttg acttcggcgt tgaggttgac ggtgtacgcc gataaagggt
     1801 ttttgcccgg tttggtgcgg ttttcattat cgtcgtccat gccgtctgaa gaatagttgc
     1861 catcgtcttc atcttcatcg ggagagccgt gggcaataca gcgcaaaact tcaaaacgcg
     1921 taaccgattg cagcttgagg aaatagacgg tgtggctgtc ggtttcgctc atcagcgcga
     1981 ccaaaacgtc caacggttcg actgcggctt ttccggcaga ctgggtatgc accatcgccc
     2041 gttgcatcac gcgttggaag ccgagcgtgg gccgggtttc gaccgtgtct aaaaggtgtt
     2101 cgggaatcag gggggtgttt tcggcaacgc tggcggcgag ctgttcggac accactttca
     2161 aatccgcgcc gcagagtttt aagacgttcg gcacggaggc atcttcttcg atgagtacca
     2221 aaagcagatg ctcgaggctg ataaattcat aatgagcctt acgcgcctcg cggtaaagct
     2281 gctgcaaaat ctgttccaat tcgggtgcaa gcatattaaa tctcctcgac aatacattgc
     2341 agcggatgcc cttcggcttt tgcccgctgc atgacttgtt gttgtttggt ttgggcaata
     2401 tcgcgcgtgt aagtgccgca caggcctttg ccttcgtgat gaaccaagag catcaccgct
     2461 accgcctgtt cttgtccgag cataaagatt tcggtcagga tttcgacgac aaattccatc
     2521 gtggtgtaat cgtcgttcaa taggaaaacg ccgtaacgtt tcggcggcag ggtgttcaga
     2581 cggtgcaaga gcgtgtcgga ttggtgttgc gcggtcatag tgtgtcccat ttgaaagccg
     2641 cgttcagacg gcatttttgt tgtattttcg gtacttttgc ctattttccc acttttttga
     2701 aaacatagct tgacgttttg tcttaacaaa tgtaaaaaga cggttaagca ggattgggca
     2761 tccgccgagt atatcaagct ggaaggaacg ctagacggct cggtttgccg tatgccttgt
     2821 tttgctgatt ttgttaattt ttgagtatag gaagtttcta atggcaaccg gtatcgtaaa
     2881 atggtttaac gacgctaaag gttttggttt catcactcct gatgaaggcg gcgaagattt
     2941 gttcgctcac ttctcagcaa tcaatatgga aggtttcaaa accctgaaag aaggccaacg
     3001 cgtctctttc gacgtaacca ccggccctaa aggcaaacag gccgccaaca ttcaggctgc
     3061 ttaattcctg atgtacggtc aaatgtatat ttgaaaacgg cgggacaggc aatgtcccgc
     3121 cgtttttgtc tgccgttttg ccggcggcgg aaaaacccta atccccgcac gccttatcct
     3181 gaacttgtgt gtaccctgtt gtggacaagt ggcttagtat tttgacggat aagggaaaat
     3241 cagtgctgat gaaaaaatgt gcaatgttgt cggcaaaagg cggtgcggca taaaacggca
     3301 aacgggcagg cacggggcaa aacgtgctgc cttcgtcttc agacggcatc ggcagggcgt
     3361 tcagcttccg gcaaccgtca tccccgcaat cagaatcgag ccgattttgt tggacgaacg
     3421 ccgcaaagcg tcatccgcca cgccgacaat gtcgcggtac atatcctgca agcgtccggc
     3481 tacggtaatc tcgtggacgg ggtaggcaat cacgccgttt tccacccaaa aacccgccgc
     3541 gccgcgcgag tagtcaccgg taatggtgtt cgcgccctgt cccatcagtt cggttaccaa
     3601 caatcccgtc cccatttctt tcagcaggtc ggattgcgtt tcgtgcgtat ggttcaaata
     3661 caggttgtgc gcgccgccgg cgttgcccgt ggtctgcata ccgagtttgc gcgcgctgta
     3721 actgctgagg aaatagcctt cgacaatgcc gttttgaatc acgaagcgcg gcgcggtggc
     3781 aacgccttcc gcatcaaaat agctgctgcg gaaagagcgg gggatgtgcg gttcttcgcg
     3841 caggttgagg aaatcgggca ggactttttt gccgatgctg tcgatcagga aactgctttg
     3901 gcggtagagc gcaccgccgg agagtgcgcc gacgaggtgt ccgataagac cgcccgaaac
     3961 ggtggtatcg aagaggacgg ggtagctgcc tgtcgggatg ctgcggctgc cgagtcggcg
     4021 caaagtgcgg cgggcggcgg tttgaccgat ggtttcgggg ctgtccatat ccggatggcg
     4081 gcaggcggaa tcgtaccagt agtcgcgctg catgccgttt tcgtcggcgg caacgacgct
     4141 gcaggaaatg ctgtggtgcg tgccttgccg gtgtgcggca aaaccgtggg tgttgccgta
     4201 aacgtattgg taatggccgg tttgcaccgc cgcgccttcg gagttttcga tgcgctcatc
     4261 ctcgttcagg gcggcttgtt cgcattgttt tgccaagccg acggcggctt ccgtatccaa
     4321 atcccattcg tggtaaaggt cggggtcgcc gatgtgtttt gccatcagac aggcatcggc
     4381 aagtccggcg caaccgtctt cggcggtgtg gcgggcgatg tcggcggcgg cgcggacggt
     4441 gtcgcgcagg gcttgttcgg agaaatcggc ggtgccagcg cggcctttgc gtttgccgac
     4501 gtaaacggta atgtccagcg acttgtcctg ctggaactcg atttgttcga tttcgcccag
     4561 ccgcacgctg acgctttgtc cgatggattc gctgaaatcg gcttcggcgg cggtcgcgcc
     4621 cgctgctttt gccaagtcga gcgtgcggcg gcagaggtcg agaagttcgg aagcggtgtg
     4681 gttgaacagc atagaaatct ttcttggtgg agcgttgcgg cattttaacc gtttcgggcg
     4741 gcaggggcaa aagcgtccgt ttgcagggcg gacggtgcaa aatgccgtct gaacgcggaa
     4801 tcattctgtt aaaatgcgcc cgtcggataa aatttgagaa tgaagatgtt tgaacaagaa
     4861 gacgaatggg tcagcaaaac ccaaatgaaa aagcagatga acggtttgca ggatttgggt
     4921 atggagctga ccaagctctc aagcgacacg ttgaaaaaaa tcggcttgga cgaagatttg
     4981 tacgaagcca tcgttaccta taaaaaaatc acatccaacg gcgcgctcaa gcggcaggcg
     5041 caatttatcg gcagactgat gcgcgatacc gatcccgcgc ccatcgaggc atttcttgcc
     5101 aagctgcgcg gcgacgatgc ggcgcacaac gcctttttgc aacgtgtgga gcggacgcgg
     5161 atggagctgc tggcggacga cggcgcgctg acgcagttta tgtccgattt tccaaacgcc
     5221 gatgcgggca agctgaggac gctcatccgc aataccaaaa aagaacagga acagaacaaa
     5281 ccgccgaaaa atttccgcgc cctgtttcaa gagttgaaaa ccgttatgga aaacggggac
     5341 gcggaaattt aggcatattt tcagacgaca tccgccgtta tttagattgg aggataaaat
     5401 gttgttccgt aaaacgaccg ccgccgtttt ggcggcagcc ttgatgttga acggctgtac
     5461 ggcaatgatg tggggcatga acgacccgtt cagccaaacg accgcctata aacacgttga
     5521 caaggatcaa atccgcgcct tcggtgtggt tgccaaagac aatgcccaat tggaaaaggg
     5581 cagtctggtg atgatgggcg ggaaatactg gttcgtcgtc aatcccgaag attcggcgaa
     5641 gctgacgggc attttgaagg ccgggttgga caaacccttc caaatagttg aggatacccc
     5701 gagctatgct cgccaccaag ccctgccggt caaactcgaa tcgcctggca gccagaattt
     5761 cagtaccgaa ggcctttgcc tgcgctacga taccgacagg cctgctgaca tcgccaagct
     5821 gaaacagctt gagtttgaag cggtcgaact tgacaatcgg actatttaca cgcgctgcgt
     5881 atccgccaaa ggcaaatact acgccacacc gcaaaaactg aacgccgatt accattttga
     5941 acagagtgtg cctgtcgata tttattacac ggttactgaa aaacataccg acaaatccaa
     6001 gctgtttgga aatatcttat atacgccccc cttgttgata ctggatgcgg cgggtgcggt
     6061 actggctttg cctgcggcgg ctctgggcgc ggtcgtggat gccgcccgca aatgaaaagc
     6121 aatgccgtct gaaaagcttt cagacggcat ttcagcctca tcatatcttg aaaaaacttc
     6181 gtcatgtcag tcaaaatcca aacccgatcc gtcaataccg acgtttttaa tcatttgctc
     6241 accgccggtg ccgatccttt aatcgcccgg ctttgtgcct cgcgcggtgt gcaaagtcct
     6301 gccgaattgg acgacaaact cgcttccctc ctgccttatc aaacgctgac aaactgcgaa
     6361 gccgccgccg gccgtttggc ggatgcggtt gaacgcaagg aaaaaatcct gattgtcgcc
     6421 gactatgatg ccgacggcgc gacggcgtgt tccgtcggta tgagcggttt ggcggcgatg
     6481 ggggcgaaag tggatttcct tgtgcccaac cgctttgagc acggctacgg cttaacgccc
     6541 gagctggctg aaatcgcggc agagcaaggt gtggatttgc tgattacggt tgataacggt
     6601 atcgccagca tcgcaggtgt ggcgagggcg caggctctgg gtttggatgt catcgttacc
     6661 gaccaccatc tgcctgccga gaccgtgccc gactgcatca tcgtcaatcc gaaccaaaaa
     6721 ggctgcggtt ttccaagcaa aagcttggcg ggcgtgggcg tgatttttta tgtattgatg
     6781 gcgttgcgtg ccgaattgcg ccgccgcaat tatttttcag acggcatcaa agagccgaat
     6841 ttgggcgaac t
//
LOCUS       DAWHXZ010000103         6798 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000103 DAWHXZ010000000
VERSION     DAWHXZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.103"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            120..1025
                     /gene="glyQ"
                     /locus_tag="WIE35_001313"
     CDS             120..1025
                     /gene="glyQ"
                     /locus_tag="WIE35_001313"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ4154224.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1102..1512
                     /locus_tag="WIE35_001314"
     CDS             1102..1512
                     /locus_tag="WIE35_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ4154225.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAE
                     DDAEAFDRFMDELIAYLEVNDFGMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLHTTPLVDGFYGDDGWENYSETYKQAD"
     gene            1533..3596
                     /locus_tag="WIE35_001315"
     CDS             1533..3596
                     /locus_tag="WIE35_001315"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ4154226.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAALGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3613..4659
                     /locus_tag="WIE35_001316"
     CDS             3613..4659
                     /locus_tag="WIE35_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ4154227.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLDILIVDDGSTDGTLAIARRFQEQDGRIRILAQPRNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTLPAGAWLDFAADGRMRRLFTL
                     RQYFGILHRLLKNR"
     gene            4701..5528
                     /locus_tag="WIE35_001317"
     CDS             4701..5528
                     /locus_tag="WIE35_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ4154228.1"
                     /translation="MQNHVISLASAAERRAHITDTFGVRGIPFQFFDALMPSERLEQV
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYITVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHWGTAGYIISK
                     KAIRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            5540..5635
                     /locus_tag="WIE35_001318"
     CDS             5540..5635
                     /locus_tag="WIE35_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ4154229.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILRRLLKNR"
     gene            5677..6519
                     /locus_tag="WIE35_001319"
     CDS             5677..6519
                     /locus_tag="WIE35_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ4154230.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT         1761 a         1823 c         1771 g         1443 t
ORIGIN      
        1 aatttaatcc actatatcat gtatgccgtg ttttcaaatc agtctgtctt tgagataaac
       61 ctatccaaac agcaatggaa acagtaaaat aaacccttta ttttatcgaa tcataagcca
      121 tgcttacctt ccaacaaatc atcttcaaac tgcaaacctt ctgggcagac aaaggctgca
      181 ccgtcatcca acccttcgac atggaagtcg gtgccggcac atcccatccc gccacctgcc
      241 tgcgcgcgct cgggcccgag ccttggtttg ccgcctacgt ccaacccagc cgccgcccca
      301 aagacggccg ctacggcgac aaccccaacc gcctgcaaca ctattaccaa ttccaagtcg
      361 ccctcaaacc cgcccccgcc aatatccaag acctttatct cgactccctg cgcgaattgg
      421 gcatcgaccc caaagtgcac gacatccgct tcgtcgaaga cgactgggaa aaccccaccc
      481 tcggcgcatg gggcttgggc tgggaagttt ggctcaacgg catggaagta acccagttta
      541 cctatttcca acaagtcggc ggcatcgact gcacgcccgt actcggcgaa atcacctacg
      601 gcatcgaacg cttggcgatg tacctgcaag gcgtggaaaa cgtctatgac ctcgtttggg
      661 caaaaacgcc ggacggcaac accgtaagct acggcgacgt gtaccaccaa aacgaagtcg
      721 aacaatccac ctacaacttc gaatacagcg atgccgattg gctgctgcgc cagttcaacg
      781 actacgaagc gcaagccaaa cgcctgcttg ccgaagaaaa cgccgccctc gccttgcctg
      841 cctacgagct ggttctcaaa gcggggcata cgttcaatct tttagacgca cgcggcgcga
      901 tttccgtaac cgagcgggca acttatattg gacgtattcg tgcattgagc cgcgccgtgg
      961 cgcaaaaata tgttgaaagc cgcgaaaaac tgggcttccc tttaatgaag gcaaatgctg
     1021 cctaaggggg cgacgatttt ttacgttcgg gcggcacgat aaggcagccg tccgtaaacc
     1081 attatatatt ttaggaaaat catgcctgtc tatttccgca ccccaagcag gaaacgcctg
     1141 cacaaaatga accgccgcca gcataaaaag ctgcatttgg gcgagtttca aacattgatt
     1201 ttcggcgttc aaggcagttt gcaccaagct gaggacgatg ccgaagcgtt cgaccgcttt
     1261 atggacgagc tgattgcgta tcttgaggta aacgactttg gaatggcggg cgggggcgat
     1321 tgcggcgatt tttcgtgcct gtttcaaaaa tccggtgccc gaagcttggg cgaaaccgag
     1381 aaaaaagcca ttatctcttg gctggcaggc cgcgaagatg tcgccacttt gcacaccacc
     1441 ccgctggttg acggttttta cggggatgac ggctgggaaa attacagtga aacctataaa
     1501 caggcagatt aaccaaattt aaagaaagcc gaatgatgac ccaaaccctt ttaatcgaac
     1561 tccttaccga agaactcccg cccaaagcac tgaataatct gggcaaccat tttgccgccg
     1621 ccgttgccga aggcttggaa aaagcgcaac tggttgacgg tgcagccgaa tttacggctt
     1681 atgcctcgcc gcgccgtttg gcggttcaag tcaaaaacgt taaagccgtt caggccgacc
     1741 aaaaaatcgt gaaaaaaggc cctgccgtgg cgaatgccat gaaagacggt gcgccgacca
     1801 aggctttgga aggtttcgca cgcggtgcgg gcgcgaaaat cgaagacttg accatcgtcc
     1861 acgacggcaa acaggatgtg tacgcctacg aatacgtcca aaccggcaaa ccgctgggcg
     1921 aacttttgga agacattatc aatcaagccg ttaagaaact gccgattccg aaagtgatgc
     1981 gttggggcag cagcacgttt accttcgtgc gccctgttca cgggctgatt gtgctgcacg
     2041 gcggcgacat cgtaaacgtc agcgttttgg gtctgcaaag cagcaatcaa accttgggac
     2101 accgcttctt gtccaacggc gaaatcacca ttgaaaacgc cgacagctac gccgaacaaa
     2161 tgcgcgggca aggcaaagtc gtcgcttcgt ttgccgagcg caaagccgcg attcagacgg
     2221 cattggaagg gcaggcgcgc agtctgaacg ccgccgtcgc cgccgatgaa gcattgttgg
     2281 acgaagtaac cgcgctggtc gaatggcctg ttgttttgga agccggtttt gaggaacatt
     2341 tcctcgccgt gccgcaggaa tgcttgattt taacgatgca gcaaaaccaa aaatactttc
     2401 cgctgctcga ccaaaacggc aagctgatga accgcttcct gctggtttcc aacctgcaaa
     2461 ccgaagaccc gtcgcacatc atccgaggca acgaacgcgt tttgcgcgcg cgcctgtctg
     2521 atgccgagtt cttctacaaa caagaccaaa aagcgacttt ggaaagccgc ctgccgaaat
     2581 tgtccagcgt ggtgtaccac aacaaaatcg gctcgcaagc cgaacgcatc gaacgcctgc
     2641 aaagcattgc cgcccacatc gccaaagcat tgggcgcgga tgccgccgct gccgaacgcg
     2701 ccgcgcgtct tgccaaagcc gacttggtaa ccgaaatggt tggcgagttc cccgaactgc
     2761 aaggcacgat gggcaaatac tatgcacgtt tggacggcga aaccgaagaa atcgccgaag
     2821 ccatcgagca gcactatcag ccgcgttttg ccggcgacaa gctgcccgaa agcaaaattg
     2881 ccgccgccgt ggcactggcg gacaagctgg aaaccctggt cggcatttgg ggcatcggtc
     2941 tgattccgac cggcgacaaa gacccctacg ccctgcgccg cgccgccttg ggtattttgc
     3001 ggatgctgat gcagtacggt ttggacgtga acgaactgat tcagacggca ttcgacagct
     3061 tccccaaagg tttgctcaac gaaaaaacgc cgtctgaaac cgccgacttt atgcaggcgc
     3121 gccttgccgt gttgctgcaa aacgattatc cgcaagacat cgtcgccgcc gtactcgcca
     3181 aacagccgcg ccgtttggac gatttgaccg ccaaactgca agccgttgcc gcgttcaaac
     3241 agctgcccga agccgccgcg ctcgccgccg ccaacaaacg cgtgcaaaac ctgctgaaaa
     3301 aagccgatgc cgcgttgggc gcggttaacg aaagcctgtt gcaacaggac gaagaaaaag
     3361 ccctctttgc cgccgcgcaa ggcttgcagc cgaaaatcgc cgccgccgtc gccgaaggca
     3421 atttccaaac cgccttgtcc gaactggctt ccgtcaaacc gcaagtcgat gcattctttg
     3481 acggcgtgat ggtaatggcg gaagatgccg ccgtaaaaca aaaccgcctg aacctgctga
     3541 accgcttggc agagcaaatg aacgcggtag ccgacatcgc gcttttgggc gagtaaccgt
     3601 tgtacagtcc aaatgccgtc tgaagccttc agacggcatc gtgcctatcg ggagaataaa
     3661 ttgcagcctt tagtcagcgt attgatttgc gcctacaacg tagaaaaata tttcgcccaa
     3721 tcattagccg ccgtcgtgaa tcagacttgg cgcaacttgg atattttgat tgttgatgac
     3781 ggctcgacgg acggcacact tgccattgcc cggcgtttcc aagaacagga cggccgcatc
     3841 cgcatcctcg cgcagccgcg caattccggc ctgattccct ctttaaacat cgggctggac
     3901 gaattggcaa agtcaggaat gggggaatat attgcacgca ccgatgccga cgatattgcc
     3961 gcccccgact ggattgagaa aatcgtgggc gagatggaaa aagaccgcag catcatcgcg
     4021 atgggcgcgt ggctggaagt tttgtcggaa gaaaaggacg gcaaccggct ggcgcggcat
     4081 cacaggcacg gcaaaatttg gaaaaagccg acccgacacg aagatattgc cgactttttc
     4141 cctttcggca accccataca caacaacacg atgattatga ggcgcagcgt cattgacggc
     4201 ggtttgcgtt acaacaccga gcgggattgg gcggaagatt accaattttg gtacgatgtc
     4261 agcaaattgg gcaggctggc ttattatccc gaagccttgg tcaaataccg ccttcacgcc
     4321 aatcaggttt catccaaata cagcgtccgc caacacgaaa tcgcgcaagg catccaaaaa
     4381 accgccagaa acgatttttt gcagtctatg ggttttaaaa cccggttcga cagccttgaa
     4441 taccgccaaa taaaagcagt agcgtatgaa ttgctggaga aacatttgcc ggaagaagat
     4501 tttgaacgcg cccgccggtt tttgtaccaa tgcttcaaac ggacggacac gctgcccgcc
     4561 ggcgcgtggc tggattttgc ggcagacggc aggatgcggc ggctgtttac cttgaggcaa
     4621 tacttcggca ttttgcaccg attgctgaaa aaccgttgaa aaacgccgct ttatccaaca
     4681 gacaaaaaac aggataaatt atgcaaaacc acgttatcag cttagcttcc gccgcagaac
     4741 gcagggcgca cattaccgat accttcggcg tgcgcggcat cccgtttcag tttttcgacg
     4801 cactgatgcc gtctgaaagg ctggaacagg taatggcgga actcgtaccc ggtttgtcgg
     4861 cgcaccccta tttgagcgga gtggaaaaag cctgctttat gagccacgcc gtattgtgga
     4921 agcaggcatt ggacgaaggt ctgccgtata tcaccgtatt tgaggacgac gttttactcg
     4981 gcgaaggcgc ggagaaattc cttgccgaag acgcttggct gcaagaacgc tttgaccccg
     5041 attccgcctt tatcgtccgc ttggaaacga tgtttatgca cgtcctgacc tcgccctccg
     5101 gcgtggcgga ctacggcggg cgtgcctttc cgctgttgga aagcgaacac tgggggacgg
     5161 cgggctatat catttccaaa aaagcgatac ggtttttcct ggacaggttt gccgccctgc
     5221 cgcccgaagg gctgcacccc gtcgatctga tgatgttcag cgattttttc gacagggaag
     5281 gagtgccggt ttgccagctc aatcccgcct tgtgcgccca agagctgcat tatgccaagt
     5341 ttcacgacca aaacagcgca ttgggcagcc tgatcgaaca cgaccgcctc ctgaaccgca
     5401 aacagcaatg gcgcgattcc cccgccaaca cattcaaacg ccgcctgatc cgcgccttga
     5461 ccaaaatcag cagggaaagg gaaaaacgcc ggcaaaggcg cgaacagttc attgtgcctt
     5521 tccaataaaa ggaggagaga tggacatcgt atttgcagca gacggcagga tgcggcggct
     5581 gtttaccttg aggcaatact tcggcatttt gcgccgattg ctgaaaaacc gttgaaaaac
     5641 gccgctttat ccaacagaca aaaaacagga taaattatgc aaaaccatgt tatcagctta
     5701 gcttccgccg cagaacgcag ggcgcacatt gccgcaacct tcggcgcgcg cggcatcccg
     5761 tttcagtttt tcgacgcact gatgccgtct gaagaactga atcggatgat ggcggaattg
     5821 gtgccgggat tggcaaaaca gcacctgttg agcgaagtgg aaaaagcctg ctttatgagc
     5881 cacgccgtat tgtggaaaca ggcattggat gaaggtctgc cctatgttgc cgtatttgag
     5941 gacgatgtcc tgcttggcaa agacgcagaa aagttccttg ccgaagatac ttggttggaa
     6001 gagcgttttg ataaggattc tgcctttatc gttcgtttgg aaacgatgtt tgcgaaagtt
     6061 attgtcagac cggataaagt cctgaattat gagaaccggt catttccttt gctggagagc
     6121 gaacattggg ggacggctgg ctatatcatt tcgcgtgagg cgatgcggtt tttcttggaa
     6181 aggtttgccg ttttgccggc agagtggatt aaagcggtag attggatgat gtttacttat
     6241 ttctttgata aggaggggat gcctgtttat caggttaatc ccgccttatg tacccaagaa
     6301 ttgcattatg ccaagttcct cagtaaaaac agtatgttgg gtagcgattt ggaaaaagat
     6361 agggaacaag aaagaagaca ccgccgttcg ttgaaggtga tgtttgactt gaagcgtgct
     6421 ttgggtaaat tcggtaggga aaagaagaaa agaatggagc gtcaaaggca ggcggagctt
     6481 gagaaagctt acggcaggcg ggtcatatcg ttcaaatagt ttgtgtaaaa tatagcggat
     6541 taaaattaga aatggataca ctgtcattcc cacgcaggcg ggaatccagt ctgttgagtt
     6601 tcagtcattt ccgataaatc accgcaacat taaatttcca gattcccgcc tgcgcgggaa
     6661 tgacggtggg gggatttctg tttttccgac aaattcctaa aacttaaaat ttcatcattt
     6721 ctacgaagac agaaaaccaa aaatagaaac ctgaaaacag aaacctgaaa acagaaacct
     6781 aaaattcgtc attcccgc
//
LOCUS       DAWHXZ010000104         6788 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000104 DAWHXZ010000000
VERSION     DAWHXZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.104"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(498..1790)
                     /locus_tag="WIE35_001320"
     CDS             complement(498..1790)
                     /locus_tag="WIE35_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ4154231.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIALTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            1867..2835
                     /locus_tag="WIE35_001321"
     CDS             1867..2835
                     /locus_tag="WIE35_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154232.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWGGTNGGTQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDIATDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(3036..4502)
                     /locus_tag="WIE35_001322"
     CDS             complement(3036..4502)
                     /locus_tag="WIE35_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ4154233.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4744..6788)
                     /locus_tag="WIE35_001323"
                     /pseudo
     CDS             complement(4744..6788)
                     /locus_tag="WIE35_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1427 a         2036 c         1739 g         1586 t
ORIGIN      
        1 tgcaaacact gccagaacgg ataaatccgg attcccgccc acttcgtcat tcccgacgcg
       61 cacgccgctt tcccgcccac ttcgtcattc ccgcgaaagc gggaatccgg tgcgttgggt
      121 ttcagtcatt tccgataaac tcctgccgca ttgtatttct agattcccgc tttcgcggga
      181 atgacggcgg atgggtttcc gtaccgtggt gtccggattc ccgacggtac gggaatgacg
      241 gcagtgcttc cttaacttag ttttatttta atcgactgta tggtggatta acaaaaatca
      301 ggacaaagcg acgaagccgc agacagtaca aatagtacga aaccgattca cttggtgctt
      361 cagcatctta gagaatcgtt ctctttgagc taaggcgagg caacgacgta ctggtttttg
      421 ttaatccact ataaatgccg tctgaaaacc tgtttgcagg ttttcagacg gcattgtttt
      481 cgtcgccggt tcaaacttca atccgattgc gacacggtaa ccggtatgcc gcgcaagcgc
      541 gcaagcagcg cgtccgcctt ctgtttctgc gcggcaaacg ccgccttgtc cgcctgcgtc
      601 aattcgggtg tcggcaatgc gactgaaaca ggattaacgg gctgcccgtt gatgcgcgcc
      661 tcgtaatgca ggtgcggccc ggtcgaacgc ccggtcgaac cgacaaaacc gatgacctcg
      721 ccgccgcgca cattgccttg tgcctgcgaa aacgcgctca agtgcgcgta cagcgtttcc
      781 acgccgttgg cgtggcgtat catcaccgcg ttgccgtatc cgcccttccg gcctttaaag
      841 gtaatcacgc cgtcggcgga agccctgacc ggcgttccct gcggtgcggc ataatcgatg
      901 cccgtgtgca gccgccatgt gtgcaggatg gggtgcatac ggtagccgaa cggcgaagaa
      961 atgcgcgtat agaccagcgg ctcgatgttg aagccgcctt tttcctgcaa caccctgccg
     1021 tcttcatcat aataattgcc gccccctccg ccttccttgt ccgaacggta atagaatgcc
     1081 tgatgccttg tgccgccctt aacgacttca gccgccaaaa tatcgcccgc cgccacctgc
     1141 tgcccgtgga aatacaggct gtcgtacatc aggcgcacgg catcgccttc cttcaaaccg
     1201 tcaaggctga agcggccggc gaaaatcccg cttaaggatt cgcggatttc gacgggcact
     1261 tccgcccgcg ccagcgaacc gcgcgccgac gttttgacca cgaccgaacg cagcgtcggc
     1321 aaaaccttca tatccgcctc agaagccgac cgccgccata tgccgccttt cttttccaaa
     1381 gcgaccagat tgcgctcgcc gtcttcgtcg gtaaaaaact gtacttcgcg cgcgccgccg
     1441 tcgccgccga ccaaaacatg aaccgactgg tcggcacgca aatgccgcaa atcggcttcg
     1501 ccgccatatt tttccgtgat tcgggcaatc tcgtcccgcg ccatacccga acgcgccagc
     1561 acgtccgcca gcgagtcgcc cggctgcacc gcctcctgca cccaatatgc cgtctgaacg
     1621 ccgctgccgc cccaagacag cggcggcaga ttttgttcca cgcgctgcgg tctgacgcgt
     1681 tccgtcctct ctgtcaaagc aatgtatgcc gccgacacca aaataatcga aacggcaagc
     1741 gcgcgcagcg cgtatttccg atgttttgcc gaaagtggga agacagccat attgttcctt
     1801 tttgacggcg tttgcgccga atcctctata atatccgccc gatccgcatt tgtcagacat
     1861 caaacgatga aactcaccct cgccctcccc tcgctcaatt tggatgaaga cgaaatccga
     1921 atacctctat gcctgcccgc cttcaacaaa atcctgcaat acggctcgct gcaccgccaa
     1981 agctgcacgg cttcggcatt ttacgcgcgt tatttgtggt gcggcagact ggcggaacgc
     2041 gcggcgcaaa gcctgaatat gccgtctgaa accgtcgcgc tcgccacgcc cgtctggcaa
     2101 aagatgggga tgcatcaggc aaatgtgctg acggcggaat atttggatgt cggtacggat
     2161 gaagcggaac ggctctgccg cgatctgtcg gcgttttacg gcgatattcc gtggcgtttc
     2221 gttcccgtcc tgcccgaatt atggctggtt tcccttcccc gcgcgtaccg ctggggcgcg
     2281 aagcccgttt tggacttggg cggattgttg ggcgcggacg atcagccgga cggagaagat
     2341 gcgttggaat ggctgcgcgt ccaaaccgaa atccaaatgt ggctggcggc gcaccccgtc
     2401 aaccataacc gcaagaaacg cgggctgccc gagttgaacg gcttatggct ttggggcggc
     2461 acaaacggcg gcacgcaagg cggcacgctt ttcgccgata cggtttggag ccgtttccac
     2521 cccgaccgcc gcgcattgcc cgacaacttc cgcgcctatg aagaaacggc ggcacacctg
     2581 cccgacacgc accatatcct gtttatggac gatttgcgcc tgaccgccct gacaggggac
     2641 agggagcggt atgcggcaat attgcagcag tgggaagaac gctggttcgc accgctttac
     2701 gaagccgtcc gcatcggcaa aatcaaacgg ctggacatcg ccacggacgg acaacacggc
     2761 ggtacgctga ccttcaaacc cgccgacagg tggaaatttt ggcgcggcgc gaaaaccttt
     2821 gacggaatct ggtgaacccg cccgccgcaa ccgcgacaat gccgtctgaa gcccgaatcg
     2881 ggtttcagac ggcattttac ctcgcatcgc cgttatgccg gaaaaaccgg aatgcgtttc
     2941 cgcacacgcg gcggcacgca aagcaccgcc cgaaagccgc cgatgcccgc cgtgataccg
     3001 ccgccggcag ggtttcctcc gaaaaggaac agcaatcaga acgttttatt aaactcgaca
     3061 aacgcccgat ttttctcgta ttcgttgaac acatcgttac tccgcgtttc gcggtgcgac
     3121 aacgtcaggc gcggcgtgat gcctttgaaa tgcaatgccc ggtgccaaag gctcaaggat
     3181 gtgttcaatt ctttatccct gcgcctttcc cctttaaaac cgctgaaaaa gccgggtttt
     3241 tcataatgcc gtttcgccgc gccgaggcgc aacagcgaag acaggccgct gccgccccat
     3301 tcctgccccc aggcaaagcg caggccgtaa cggttgaaat tgtcgccccg gtcggcgggg
     3361 ttgcgctcgc ggtaaaaatc caaaccgccc atccaatatt ggcgcgcatt ccggtaaaac
     3421 accagcgaat tggaaatttg caaatgggta ttgtcggaac gcgcccggcg cgtattcttc
     3481 aaacgccccc actccgccga agacaacgtt tgccatttcg gggtttgcca acggttgaaa
     3541 taaaggcgtg cgccgttggt gtaagaataa gcgtcgttgc cgtaggtgcg gcgttcgtgg
     3601 aacactgcca gcccggcatc tttgcgccgg tcggcaaaac cgatgccgcc ggaaacgcct
     3661 gccgtcatat cgttgaattt cttattcccc ggataaaccc tgccggacac gtcgccgccc
     3721 gccgtcgtgt accagccgtt tttcagcgac cattttttct ccgcgccgag ccggtaattg
     3781 accgccgtgc cgtccacctg tttcgggaaa gtccatttgc cgtactgctg ccgtttcggg
     3841 gcttggttga tattgtgttc gcgggtgacg ctgaagccgc catttacctt ccacgcatcg
     3901 cgttcgcgca atgccttgcg gtacagctcg acctgctcca tcagctgcgg cggcaggttt
     3961 tccgccttca ggcggtcgaa ctggtctgcc gccgcctcgt tctgcctgtt ttcaaacaat
     4021 gccgccgcca aacgcatacg gacggcgggc gcgtcgggtt gggcggcaat caattcccgg
     4081 taatgggaaa tcgcctcctt caccctaccg tctgcctgcg ccaaaatccc ttgtgcataa
     4141 agtgccaaca tcttatcctg ctgcgcctgt tgtaggtaaa tcggcaaaat aacgcggata
     4201 ccggcaatat tgtttgagac cactgcggaa tacatcgcgc gggacaacaa ttcgggattt
     4261 ttcagcaggg tttctccgtc aatctgcaaa acttttcctt tttcccgcac ctgccccggt
     4321 accttctccc tgtcgattgg tttgacctcc gcttcatgaa gcctgaactc gggacggctt
     4381 ctcaaatccg gttcgcgcgg tgtttcttcg gcatatgccg cgcttgccaa taaaggcagc
     4441 agcagcatcc atctgtttcg tgcaggcata aatgtcttcc cacaaaaata aaaaataacc
     4501 attatcaatc ggcggattgt atcagttttt tatgccgctg ttccaaaccc tgccgcccga
     4561 tgccgcgaca atgccgtctg aagccagaat cgggtttcaa acggcggcaa aaaaccggcg
     4621 gttttccgcc ggtttcatca tgatgccccg cccttcgggg cggcagggtt cggattggtt
     4681 tgcggggcgg cgcgcggggc cgtgccggtg tgccgcctgt gcgtcaaaac atttcccgac
     4741 gcatcaaaac agccatttga gcgacaatgc ggcttctttg tcgccgtccg tccttttgcc
     4801 gtatccgatg cgtgcggaca tatggccttt ccaaccggct tcaatgccga accgcccttc
     4861 gagcgccgtc ctgcctgcca gcgtctgttt ttcgccgtcc atttccacgc cgaaagattt
     4921 tgacctgtgc aaaacattaa aagcggcgaa aggctgaaga ttgacaccgt tacgcaaagc
     4981 aaaacgggtt tttgcccgaa taccggcgcg gctttgccac tgaccgctgc cgagcagtcc
     5041 gaccgccgtc ccctcgctgt cggtaaagcc gccgtttacg cccaagtagg taaactgagc
     5101 ctgcggttgc aggtaaaacc gcacattatt gccttttccg acaacgcctt ccgccacaag
     5161 cgcgttgtag ccgccttcga cagaagccgt ccaacctttg gttttgtagc gttccacacg
     5221 gttttcatca ttgatgcggt gtttgaaacg ttggtattgc aaccagccgt ccaaatacgc
     5281 acccgtttgt ttatcgcgca actgatgcca cgcagcataa acacccccgc cataaccatg
     5341 caaataactg cctgccgcac cgcctttgcc gttgactgat gcgtgctggc cggccctgcc
     5401 gcccatcacg ccgattgcca gccggctgcc ttcattttgc cgtacaaaca cctcgccgcc
     5461 gatttgcacg cctttgcgcc gcccgtccgc agccgcgccg ccccgtatat tttgatgcga
     5521 ccggccgccg atgaagcgca gccacaattt ttgacgggac ggatcggcgg catacacgtc
     5581 gtctcccctg tcggcggcac gcagcccaaa caaagtattt gccgcataag cctgttgggc
     5641 atataaaacc gcttccgcct cggggatagc caaacgctgc acgcccaagc aaaatccgcc
     5701 ttcgcattgc ttgagcaata attcgtacag tccctgccgg taattttgtt taaacgcaaa
     5761 cgaattaggg tctgcctttt ccgccgcaat cagtttgcgc ccgtcgagcg ttttcagatt
     5821 ttccggccgg cgttccaaaa ccacgccgga ttgtccgctg actttgtctt caaaggtcag
     5881 ggcgggttcg tccgactccc gcacgtcgta gccgaacagg aaatccgcgc cgccctgata
     5941 cgctttatgg acggtcagtt tttcatcttt cgtgttcagg cggatccggg cattatcgga
     6001 agtttccagc atatggatgt cggaatggcg gcgcggttcc caaagggaat ctttcagcgt
     6061 catcttggtc agttccaaca cctgtccggc gacgtgggat ttgttttcga ttttgacgtg
     6121 taatttttta ttgaagatgt tgttgagact agtctgtgat gtattactat gaggagtgag
     6181 tacggaatct tctaccaatt tattaattaa atcggggttt ttctgtttga gtatactaaa
     6241 ttttggtcca agctgttcta tatacgcctc ctcagtccgt tttttatttt cttcccaagc
     6301 ttcgggtctt gttttgtata aatcctgtaa ttgcttctta accgcatcct ttaaattatc
     6361 cccgtaagta tctgtaatat atttcttaac aacgggcata ttatcgacgg cgagtatgcc
     6421 gctattgtag tttttacctt ggatattgat ttcgtcatac ccatattcgg atgaattttg
     6481 atcggtttta ccgctggaaa aataatggga agtgccgggt ttcggcttgt ctgaaaatga
     6541 aaaatcggca tcgtcgccga ttttggtaga acgagtattt gaaccaagct gagctctgaa
     6601 cataccgaag caatcaaaag atatgctgca cgggccgcgc caaacgtggg aatttctgtc
     6661 ttccgccgct tctaaaataa atttgttttt aatctcggct tctttgacgg gatcgtacgc
     6721 atccgaacct caaacatatg gcgtgcaaaa cagcgataaa acagataatg ttaatagatt
     6781 aacccgac
//
LOCUS       DAWHXZ010000105         6660 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000105 DAWHXZ010000000
VERSION     DAWHXZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.105"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            285..2132
                     /locus_tag="WIE35_001324"
     CDS             285..2132
                     /locus_tag="WIE35_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ4154234.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNTLENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATVDL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLTGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMELKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            2187..6356
                     /locus_tag="WIE35_001325"
     CDS             2187..6356
                     /locus_tag="WIE35_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ4154235.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADCNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRINPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     HFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNVAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTRKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNGKGK"
BASE COUNT         1571 a         2135 c         1748 g         1206 t
ORIGIN      
        1 aaggttgcgt tgatttgccc tatgcagtgc cggacaggct ttgcttgatc attcggcgca
       61 acggtttaat ttattgaacg aaaataaatt tatttaatcc tgcctatttt ccggcactat
      121 tccgaaacgc agcctgtttt ccatatgcgg attagaaaca aaatacctta aaacaagcag
      181 atacatttcc ggcgggccgc aacctccgaa ataccggcgg cagtatgccg tctgaagcgt
      241 cccgccccgt ccgaacagtg ttaaaatcga aatccgccac accgatgcac gacacccgta
      301 ccatgatgat caaaccgacc gccctgctcc tgccggcttt atttttcttt ccgcacgcat
      361 acgcgcctgc cgccgacctt tccgaaaaca aggcggcggg tttcgcattg ttcaaaaaca
      421 aaagccccga caccgaatca gttaaattaa aacccaaatt ccccgtccgc atcgacacgc
      481 aggatagtga aatcaaagat atggtcgaag aacacctgcc gctcatcacg cagcagcagg
      541 aagaagtatt ggacaaggaa cagacgggct tcctcgccga agaagcaccg gacaacgtta
      601 aaaccatgct ccgcagcaaa ggctatttca gcagcaaagt cagcctgacg gaaaaagacg
      661 gagcttatac ggtacacatc acaccgggcc cgcgcaccaa aatcgccaac gtcggtgtcg
      721 ccatcctcgg cgacatcctt tcagacggca acctcgccga atactaccgc aacacgctgg
      781 aaaactggca gcagccggta ggcagtgatt tcgatcagga cagttgggaa aacagcaaaa
      841 cttccgtcct cggcgcggta acgcgcaaag gctacccgct tgccaagctc ggcaacaccc
      901 gggcggccgt caaccccgat accgccaccg tcgatttgaa cgtcgtcgtg gacagcggcc
      961 gccccatcgc cttcggcgac tttgaaatca ccggcacaca gcgttacccc gaacaaatcg
     1021 tctccggcct ggcgcgcttc caaccgggca cgccctacga cctcgacctg ctgctcgact
     1081 tccaacaggc actcgaacaa aacgggcatt attccggcgc gtccgtacaa gccgacttcg
     1141 accgtctcca aggcgaccgc gtccccgtca aagtcagcgt aaccgaggtc aaacgccaca
     1201 agctcgaaac cggcatccgc ctcgattcgg aatacggttt gggcggcaaa atcgcctacg
     1261 actattacaa cctcttcaac aaaggctata tcggctcggt cgtctgggat atggacaaat
     1321 acgaaaccac gcttgccgcc ggcatcagcc agccgcgcaa ctatcggggc aactactgga
     1381 caagcaacgt ttcctacaac cgttcgacca cccaaaacct cgaaaaacgc gccttctccg
     1441 gcggcatctg gtatgtgcgc gaccgcgcgg gcatcgatgc caggctgggg gcagagtttc
     1501 tcgcagaagg ccggaaaatc cccggctcgg atgtcgattt gggcaacagc cacgccacga
     1561 tgctgaccgc ctcttggaaa cgccagctgc tcaacaacgt gctgcacccc gaaaacggcc
     1621 attacctcga cggcaaaatc ggtacgactt tgggcacatt cctgtcctcc accgcgctga
     1681 tccgcacctc tgcccgcgca ggttatttct tcacgcccga aaacaaaaaa ctcggcacgt
     1741 tcatcatacg cggacaagca ggttacaccg ttgcccgcga caatgccgac gttccttcgg
     1801 ggttgatgtt ccgcagcggc ggcgcgtctt ccgtgcgcgg ttacgaactc gacagcatcg
     1861 ggcttgccgg cccgaacgga tcggtcctgc ccgaacgcgc cctcttgacg ggcagcctgg
     1921 aataccaact gccgtttacg cgcacccttt ccggcgcggt attccacgat atgggcgatg
     1981 ccgccgccaa tttcaaacgt atggagctga aacacggttc gggactgggc gtgcgctggt
     2041 tcagcccgct cgcgccgttt tccttcgaca tcgcctacgg gcacagcgat aagaaaatcc
     2101 gctggcacat cagcttggga acacgcttct aaaccgatac ggccgcttca gacggcattg
     2161 cagcaaacta ttttgaaaca gacattatga ccgataccgc accgacagat accgatccga
     2221 ccgaaaacgg cacgcgcaaa atgccgtctg aacaccgccc caccccgccg gcaaaaaaac
     2281 gccgcccgct gctgaagctg tcggcggcac tgctgtctgt cctgattttg gcagtatgtt
     2341 tcctcggctg gctcgccggc acggaagcgg gtttgcgctt cgggctgtac caaatcccgt
     2401 cttggttcgg cgtaaacatt tcctcccaaa acctcaaagg cacgctgctc gacggcttcg
     2461 acggcgacaa ctggtcgata gaaaccgagg gggcagacct taaaatcagc cgcttccgct
     2521 tcgcgtggaa accgtccgaa ctgatgcgcc gcagcctgca cattaccgaa atttccgccg
     2581 gcgacatcgc catcgttacc aaaccgactc cgcctaaaga agaacgcccg ccgctcagcc
     2641 ttcccgacag catagacctg cctgccgccg tctatctcga ccgcttcgag acgggcaaaa
     2701 tcagcatggg caaagccttt gacaaacaaa ccgtctatct cgaacggctg gatgcttcat
     2761 accgttacga ccgcaaagga caccgccttg acctgaaggc cgccgacacg ccgtggagca
     2821 gttcgtcggg gtcagcctcg gtcggcttga aaaaaccgtt tgccctcgat accgccattt
     2881 acaccaaagg cggactcgaa ggcaaaacca tacacagtac ggctcggctg agcggcagcc
     2941 tgaaggatgt gcgcgccgaa ctggcgatcg acggcggcaa tatccgcctc tcgggaaaat
     3001 ccgtcatcca cccgtttgcc gaatcattgg ataaaacatt ggaagaagta ctggtcaaag
     3061 ggttcaacat caatccggcc gccttcgtgc cttccctgcc cgatgccgga ctgaatttcg
     3121 acctgaccgc catcccgtcg ttttcagacg gcatcgcgct ggaaggttcg ctcgatttgg
     3181 aaaacaccaa agccggcttt gccgactgca acggcatccc cgtccgtcag gttttaggcg
     3241 gctttgtcat ccggcaggac ggcacggtgc atatcggcaa tacgtccgcc gccctgctcg
     3301 gacggggcgg catcaggctg tcgggcaaaa tcgacaccga aaaagacatc ctcgatttaa
     3361 atataggcat caactccgtc ggcgcggaag acgtactgca aaccgcgttc aaaggcaggt
     3421 tggacggcag catcggcatc ggtggcacga ccgcctcgcc caaaatctct tggcaactcg
     3481 gcatcggcac ggcgcgcacg gacggcagcc tcgccattgc aagcgacccc gcaaacggac
     3541 agcggaaact ggtgctcgac accgtcaaca tcgccgccgg gcaaggcagc ctgaccgcgc
     3601 aaggctatct cgagctgttt aaagaccgcc tgctcaagct ggacatccgt tcccgcgcat
     3661 tcgacccttc gcgcatcaat ccgcaacttc cggcaggcaa tatcaacggc tcaataaacc
     3721 ttgccggcga actggcaaaa gagaaattca caggcaaaat gcggttttta cccggcacgt
     3781 tcaacggcgt accgattgcc ggcagtgccg acattgttta cgagtcccgc caccttccgc
     3841 gtgccgccgt cgatttgcgg ctggggcgga acatcatcaa aacagacggc ggcttcggca
     3901 aaaaaggcga ccggcttaac ctcaatatca ccgcacccga tttatcccgt ttcggtttcg
     3961 gactcgcggg gtctttaaat gtacgcggac acctttccgg cgatttggac ggcggcatcc
     4021 gaacctttga aaccgacctt tccggcgcgg cgcgcaacct gcacatcggc aaggcggcag
     4081 acatccgttc gctcgatttc accctcaaag gttcgcccga cacaagccgc ccgatacgcg
     4141 ccgacatcaa aggcagccgc ctttcgctgt cgggcggagc ggcggttgtc gataccgccg
     4201 acctgatgct ggacggcacg ggcgtgcagc accgcatccg cacacacgcc gccatgacgc
     4261 tggatggcaa accgttcaaa ttcgatttgg acgcttcagg cggcatcaac agggaactta
     4321 cccgatggaa gggcagcatc ggcatcctcg acatcggcgg cgcattcaac ctcaagctgc
     4381 aaaaccgtat gacgctcgaa gccggtgcgg aacgcgtggc ggcaagtgcg gcaaattggc
     4441 aggcaatggg cggcagcctc aacctgcaac acttttcctg ggacaggaaa accggcatat
     4501 cggcaaaagg cggcgcacgc ggtctgcata tcgccgagtt gcacaatttc ttcaaaccgc
     4561 ccttcgaaca caatctggtt ttaaacggcg actgggatgt cgcctacggg cgcaacgcgc
     4621 gcggctacct caatatcagc cggcaaagcg gcgatgccgt attgcccggc gggcaggctt
     4681 tgggtttgaa cgcattttcc ctgaaaacgc gctttcaaaa cgaccgcatc ggaatcctgc
     4741 ttgacggcgg cgcgcatttc gggcggatta acgccgattt ggacatcggc aacgccttcg
     4801 gcggcaatat ggcaaatgca ccgctcggcg gcaggattac cgcctccctt cccgacttgg
     4861 gcgcattgaa gccctttctg cccgccgccg cgcaaaacat taccggcagc ctgaatgccg
     4921 ccgcgcaaat cggcggacgg gtcggctctc cgtccgtcaa tgtcgccgtc aacggcagca
     4981 gcaactacgg gaaaatcaac ggcaacatca ccgtcgggca aagccgctct ttcgataccg
     5041 cgcctttggg cggcaggctc aacctgaccg ttgccgatgc cgaagtattc cgcaacttcc
     5101 tgcccgtcgg acaaaccgtc aaaggcagcc tgaatgccgc cgtaaccctc ggcggcagca
     5161 tcgccgaccc gcacttgggc ggcagcatca acggcgacaa gctctattac cgcaaccaaa
     5221 cccaaggcat catcttggac aacggttcgc tgcgttcgca tatcgcaggc aggaaatggg
     5281 taatcgacag cctgaaattc cgtcacgaag ggacggcgga actctccggt acggtcggta
     5341 tggaaaacag cggacccgat gtcgatatcg gcgcggtgtt cgacaaatac cgcatcctgt
     5401 cccgccccaa ccgccgcctg acggtttccg gcaacacccg cctgcgctat tcgccgcaaa
     5461 aaggcatatc cgttaccggg atgattaaaa cggatcaggg gctgttcggt tcgcaaaaat
     5521 cctcgatgcc gtccgtcggc gacgatgtcg tcgtattagg cgaagtcaaa aaagaggcgg
     5581 cggcaccgct ccccgtcaat atgaacctga ctttagacct caatgacggc atccgcttcg
     5641 ccggctacgg cgcggacgtt accataggcg gcaaactgac cctgaccgcc caatcgggcg
     5701 gaagcgtgcg gggcgtgggc acggtccgcg tcatcaaagg gcgttataag gcatacgggc
     5761 aggatttgga cattaccaaa ggcacggtct cctttgtcgg cccgctcaac gaccccaacc
     5821 tcaacatccg cgccgaacgc cgcctttccc ccgtcggtgc gggcgtggaa atattgggca
     5881 gcctcaacag cccgcgcatt acgctgacgg caaacgaacc gatgagtgaa aaagacaagc
     5941 tctcttggct catcctcaac cgcgccggca gcggcagcag cggcgacaat gccgccctgt
     6001 ccgcagccgc aggcgcgctg cttgccgggc aaatcaacga ccgcatcggg ctggtggatg
     6061 atttgggctt tacccgcaag cgcagccgca acgcgcaaac cggcgaactc aaccccgccg
     6121 aacaggtgct gaccgtcggc aaacaactga ccggcaaact ctacatcggc tacgaataca
     6181 gcatctccag cgcggaacag tccgtcaaac tgatttaccg gctgacccgc gccatacagg
     6241 cggttgcccg tatcggcagc cgttcgtcgg gcggcgagct gacatacacc atacgtttcg
     6301 accgcttctc cggttcggac aaaaaagact ccgccggaaa cggcaaagga aaataagcgg
     6361 ttttcagacg gcgcgccgcc aaaccggaca tttgaaaacc tgcttttcca ccgtccgccg
     6421 ccgccgtccg cctgcaaggg aacagaatcg atatagtgaa ttaacaaaaa tcaggataag
     6481 gcgacgaagc cgcagacagt acaaatagta cggaaccgat tcactcggtg cttgagcacc
     6541 ttagagaatc gttctctttg agccaaggcg aggcaacgcc gtaccggttt ttgttaatcc
     6601 gctatattcc gccatctcta agatttacag cgatacacag gtaatttaag gaatgcccga
//
LOCUS       DAWHXZ010000106         6650 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000106 DAWHXZ010000000
VERSION     DAWHXZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.106"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..184)
                     /locus_tag="WIE35_001326"
     CDS             complement(<1..184)
                     /locus_tag="WIE35_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ4154236.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADE"
     gene            complement(417..2474)
                     /gene="ppk1"
                     /locus_tag="WIE35_001327"
     CDS             complement(417..2474)
                     /gene="ppk1"
                     /locus_tag="WIE35_001327"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ4154237.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAQEGIHFYRRRNWTGAQKKWIESYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPSYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRATPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(2643..3086)
                     /locus_tag="WIE35_001328"
     CDS             complement(2643..3086)
                     /locus_tag="WIE35_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ4154238.1"
                     /translation="MPFRLFLGILCDLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(3093..3608)
                     /locus_tag="WIE35_001329"
     CDS             complement(3093..3608)
                     /locus_tag="WIE35_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ4154239.1"
                     /translation="MKIKQIVKPGLAVLAAGILSACATKSNVKADGATDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVHEWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            3875..6505
                     /locus_tag="WIE35_001330"
     CDS             3875..6505
                     /locus_tag="WIE35_001330"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ4154240.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     AAAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYADKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAEAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFKRLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGSQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEASLVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
BASE COUNT         1464 a         1797 c         1935 g         1454 t
ORIGIN      
        1 cctcatcggc agccaaggcc aaatgatggt tgataaccgt gcggatttta cggtagaaca
       61 gtgctgccga attgggatgg atacccaaaa tatcggcggc agaacgggcg gtaacttcca
      121 gtacaaaaaa acggagcagt tctttctgta cttttttctt taatttgcag tgcgttatct
      181 tcatatttcg agggtaacat atctgctaat ctagtacagc ccctaaaatt taatccacta
      241 taatgccgtc tggaatgcgg cggcttgggg ttgggagcgt ataggggaag tgcttgtgca
      301 ggaatggcga aggtgtgttt ttgactccgg tccgccctgt ttagattgct gtaaaaccat
      361 gccgtctgaa acggaaaggt ttcagacggc atggtttttg attggggcgg tgcgggtcag
      421 cctccgagca gtgtccacaa atcgttttgc aggtcggctt cgctttcgcc ctcggcgggc
      481 gtggcgcgga tatagccgcc gtcgggctgc atcagccacg cgtgcgtatt gtctgccaat
      541 gccatttcca aaccttcgcg gatgacgcgt tttttgagtt cgggcgcggt aatcggcgcg
      601 gcggtttcga tgcggcggaa gaagttgcgc cccatccaat ccgcgctgga gataaaggta
      661 tcgtccgcgc cgttgttgtg gaagcaatac acgcgcgcgt gttcgagctg cctgccgatg
      721 atggagcgga cgcggatgtt ttcggacaag ccttttacac ccgggcgcaa ggtgcacata
      781 ccgcgcacaa tcaaatcgat ttgtacgcct gccgcgcttg cccgatacag ggcttcgatg
      841 acggtcggtt cgatgagcga attcatcttg gcggtaatcc gcgccggttt gccggctttt
      901 gcgtgttcgg tttcgcgtgc gatgcggtcg ataaccattt tgtgcagggt aaacgggctt
      961 tggtagagtt tgttcagccg cccgggtttg cccaagcctg tgatttccat aaacaatgtg
     1021 ttcacatcgg cggtgatttg ttcgtcggcg gtaatgatgc cgaagtcggt gtagatgcgc
     1081 gatgtgcctt ggtggtagtt acccgtgccg aggtgggcgt aacgcttgag tacgccgtct
     1141 tcgcggcgga tgaccagtgc catcttggcg tggactttgt agccgaacac gccgtacacg
     1201 acgtgcgcgc ccgcctcttc gagctgcttc gcccagttga cgttgttggc ttcgtcaaaa
     1261 cgcgccatca gttcgacgac gacggttact tgtttgccgg cgagtgccgc cttcatcagg
     1321 gcgcggacga gttcggaacg cgtgccggtg cggtaaatcg tcattttgac ggcaagcacg
     1381 tcggggtcgg cggcagcttc gcgcatcatt tcgacaacgg ggtcgaacga ttggtagggg
     1441 tggtgcagca ggatgggcga ttggcgcacc aaatcgaata tggacgcggt tttgcccaag
     1501 gctttcagtc tgcccggcgt gtgtgtggga aatttcaaat cggggcggtt gactaggtcg
     1561 gggacggcgt tgaggcgcac gaggttgacc gggcctttga cctgatagag ttcggcggcg
     1621 gtcagtttga attgcgcgag cagaaagtcg cggatgtagg agggacaggt gtcggcgact
     1681 tcgagccgca cgccgtcgcc gtattcgcgg tcgtgcagtt cgttttgaat ggcggcgcgg
     1741 aggttttgta cgtcttcttc gtcaacggtc aagtcgctgt cgcgcgtgag gcggaactgg
     1801 tggcagcctt tgacgttcat gcccgggaag agtttgccga cgtgggcgtg caggatggag
     1861 gagaggaaga cgaagccgtg tccgccgcca cacagttcgg acggcagggg aacaacgcgc
     1921 ggcaggatgc gcggtgcctg cacaatcgcc atccccgaag gcctgccgaa cgcgtccgtg
     1981 ccgtcgagtt cgacggcgaa gttgagcgat ttgttcagcg gtcgcgggaa ggggtgggac
     2041 gggtcgagtc cgatgggggt cagaatcggc agcaattcgc ggtcgaaata gctttcgatc
     2101 cactttttct gcgcgcccgt ccagttgcgg cggcggtaaa aatggatgcc ttcttgtgcc
     2161 agctcgggct gaaggacgtt gttgaacagg tcgtactggt gccgtatcag ggagcgcgcc
     2221 gcttcggtaa cgtcggcgat ggtttcagac ggcattttgc cgttgtccag cctgcgccgg
     2281 gggtgcagct tgttttcacg cttgagccac gccatgcgga cttcgaaaaa ctcgtcaaga
     2341 ttggacgaca cgatgcacag gaagcgcagg cgttccaaaa gggggacgtt tttgtcttcc
     2401 gcctgtgcca acacgcggcg gttgaatgcc agcaggctta gttcgcggca gaggatgcgg
     2461 ttttgttcgg gcataaggtt ctcccaaaga tggttgttgt tcggtcggag cgggcaggca
     2521 atgccgtctg aaggcttggc ggcatctgcg ccggtttcag acggcatgac gtggcggaca
     2581 gtctgtttca ggcatcgggg caatgtgaaa acagataccg gcgcgtgcgg tatccgtctg
     2641 tttcaaatca cttccaacac aaaataatga cgcagttttt cgtacaccgc atcgctgacg
     2701 ttgatgcagc cgttggtcat aatcctgtcg gacacggacg gggaggcgat gcgttcgttc
     2761 cgcctttccg acggtatctg attccaaacg cggtgcaggg cgaaaagaaa atcaccctcc
     2821 tgcttgaaac cgatgacttc gccgccgtat ccaggcttgt cggtactgtt cagcgtcagc
     2881 ccgaacgtcc ccttgggcgt ggcaacaccg atgaggacgg ggtggcactg cctgtcatcg
     2941 gcaaaacaga gttccgcttt ggatgtgttt acgatgactt ttttcttttg aatataagcg
     3001 ttgaccgcat ccggttgccc ctgtgcatat acgggggcaa ccatcgatag gtcgcacaat
     3061 atccctaaaa acaggcggaa cggcatgtcg gattattggc ggatacgttt cggttttgcc
     3121 ggcacgattt cgcggatgat gacttgcggc tcggctttgg gtgcgggcgg cggacaggcg
     3181 gcatctttcg ggaagacggg gttccagtag aagctgcggg caaatttgtc tttatcgaaa
     3241 atcactttgt attggcaggt agtaacatct tctacgccgg acgtgttttc agggtcgata
     3301 cctacgcccg gggtatggaa gtggaacagg taatcccatt cgtgtacgcc gtacatgcct
     3361 tcgtcgtaat gcgggcgacc caggattttg tagatgtcgt ctttggtcag gccggggcgc
     3421 atctgatcca gttcgtcata ggtcgggaat gtgccgcgct tgttgtcgag cgttacggaa
     3481 tagggtttcg ggaaaaccgg attgtcggtc gctccgtcgg ctttgacgtt gcttttggtt
     3541 gcgcaggcag acagaatgcc cgccgccaat actgccaagc ccggtttgac gatttgtttg
     3601 attttcatat gcagaatcct tttttacccg atgcccgtct gccttttgtt cggaagagcc
     3661 gcatcaggag agttttaaac atttgatttt ggtttgtaat attagcataa aaaacgtgcg
     3721 tatcagtaaa cgcggtgtat ttgtacggca tacggaatga tgcgcgtgcg aatttacgca
     3781 tcctgccggc aatttgccga ttcgccgaca tcggcaacct gttataattc ctcctttaaa
     3841 ttcctaacgt tttcaagcga aaaacagaat gactatgcaa gaacaatatc aacccgccgc
     3901 catcgagcct gcggcgcaga aaaaatggga cgacgcccgt attttcaacg tctccgaaga
     3961 cgcttccaaa cccaaatact actgcctctc catgttcccc taccccagcg gcaagctgca
     4021 tatggggcat gtgcgcaact acaccatcgg cgacgtattg agccgcttca aacttttaaa
     4081 cggcttcaac gtcatgcagc ctatgggttg ggacgcgttc ggcatgccgg cggaaaacgc
     4141 ggcgatgaaa aacaacgtcg cccctgccgc ttggacgtac gataacatcg aatacatgaa
     4201 aacccagctc aaaagcctgg gttttgcgat tgactgggcg cgcgaaaccg caacctgcaa
     4261 acccgaatac taccgctggg aacaatggct gtttaccaag ctgtttgaaa aaggcatcgt
     4321 ttatcgcaaa aacggcacgg taaactggga cccggtcgac caaaccgtcc ttgccaacga
     4381 gcaagttatc gacggacgcg gttggcgttc gggcgcgttg atcgaaaaac gcgaaatccc
     4441 gatgtattac ttcaaaatca ccgattacgc cgaagagctg ctcaacgact tggacaagct
     4501 ggaacactgg cctgaacaag tcaaaaccat gcagcgcaac tggatcggca aatctcgcgg
     4561 catgaccgtg cgctttgctg tttcagacga cagcaaacaa ggcttggaag gcgattacgc
     4621 gaaattcctg caagtttata ccacccgccc cgacacgctg atgggtgcga cttatgttgc
     4681 cgttgccgcc gagcatccgc tggcagccgc cgcagccgcc gacaaacccg aattgcaggc
     4741 atttattgcc gaatgcaaag ccggttcggt tgccgaagcc gatatggcga cgatggagaa
     4801 aaaaggcgtg ccgaccggtc gctacgtcgt caacccgctc aacggcgaca agctggaagt
     4861 gtggattgcc aactatgtat tgtggggcta cggcgacggc gcagtgatgg cggttccggc
     4921 gcacgacgaa cgcgattttg agttcgccgc caaatacaat ctgcctaaaa aacaagtcat
     4981 tgccgtcggc gacaacgcat tcgacgcaaa ccgatggcaa gaatggtatg ccgacaaaga
     5041 aaacggcgta ttggtcaaca gcggcgactt ggacggcttg gattttcaga cggcattcga
     5101 tgccgttgcc gccaagctgc aaagccaagg cgcgggcgaa ccgaaaaccc aataccgcct
     5161 gcgcgactgg ggcatttcgc gccaacgcta ctggggctgt ccaattccca tcgtccattg
     5221 tgaaaaatgc ggcgacgtac ccgtcccagc cgaccaactg cccgtcgttc tgcccgaaaa
     5281 cgtcgtaccc gacggcatgg gttcgccgct ggcaaaaatg cccgagtttt acgaaactgc
     5341 ctgcccgtgc tgcggcggcg cggcgaaacg cgaaaccgac accatggaca ccttcatgga
     5401 atcaagctgg tatttcttcc gctatatgtc gcccaagttt tcagacggca tggtatcggc
     5461 agaagccgcg aaatactggg gcgcggtcga ccaatacatc ggcggtatcg aacacgcgat
     5521 tttgcatctc ttgtacgcgc gtttcttcac caaactgatg cgcgacgaag gtttggtcaa
     5581 tgttgacgaa ccgttcaaac gcctgctcac gcaaggcatg gtcgtctgcg aaacctacta
     5641 ccgcgaaaac gacaaaggcg gcaaagactg gatcaacccc gccgatgtcg agctgacgtt
     5701 cgacgacaaa ggccgccccg tttccgccgt cctcaaagcc gacggcctgc ccgtcgtcat
     5761 cagcggcacg gaaaaaatgt ccaagtccaa aaacaacggc gtcgatccgc aagaactgat
     5821 taacgcctac ggcgcggaca ccgcccgcct gttcatgatg tttgccgcac cgcccgaaca
     5881 gtccctcgaa tggagcgaca gcggcgtcga aggcgcacac cgcttcctgc gccgcctgtg
     5941 gcgcaccgtt tacgaatacc tgaaacaagg cggcgcggtc aaagcgttcg caggcagcca
     6001 agatggtttg tctaaagaac tcaaagacct gcgccacaaa ctgcattcca ctatcgccaa
     6061 agtcagcgac gactacggcc gccgccagca gttcaacacc gccatcgccg ccgtgatgga
     6121 attgctcaac caatacgaca aaaccgacac cggcagcgaa caaggccgcg ccgtcgccca
     6181 agaagtattg gaagccgccg tacgtctgct gtggcccatc gtgccgcaca tctgcgaaac
     6241 cctgtggagc gaattgaacg gcgcgaaatt gtgggaagca ggctggccga cagtcgacga
     6301 agcctccttg gtcaaatccg aaatcgaagt catggttcaa gtcaacggca aactgcgcgg
     6361 aaaaatcacc gtcgccgccg acgcatccaa agccgacctc gaagccgccg cactcgccac
     6421 cgagggcgca gtgaaattca tggaaggcaa gcccgccaag aaaatcatcg tcgtgccggg
     6481 cagactggtg aacatcgtcg tctaaccgct tttaaggttt agccacacgg ataaaggccg
     6541 agacctttgc aaaattccct tccctcccga cagccgaaac ccaaacacag attttcggct
     6601 gttttcgccc caaatacctc ctaattctac ccaaacgccc ccttaatcct
//
LOCUS       DAWHXZ010000107         6628 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000107 DAWHXZ010000000
VERSION     DAWHXZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.107"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1410
                     /locus_tag="WIE35_001331"
     CDS             <1..1410
                     /locus_tag="WIE35_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ4154241.1"
                     /translation="LGQERKAFADQYALERQIRQRIETDLEESRQTVRDVQNDLSDVG
                     NRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIEQERLASEEK
                     LSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERIHGFGELVKQ
                     TYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWGEMILETVLE
                     NSGLQKGREYVVQAAAVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVSLTAYVRYTQ
                     AADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVEPAYLLALQN
                     DAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGGKLYDKFVGF
                     VQTLESVGKGIDQAQSSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQLARDLTEHA
                     NETTALSESLEYAAEDEAV"
     gene            complement(1617..2249)
                     /locus_tag="WIE35_001332"
     CDS             complement(1617..2249)
                     /locus_tag="WIE35_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ4154242.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTRKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFSPNRWLEEGETLTVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            2341..3165
                     /locus_tag="WIE35_001333"
     CDS             2341..3165
                     /locus_tag="WIE35_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ4154243.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQTKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     IGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            3234..3797
                     /locus_tag="WIE35_001334"
     CDS             3234..3797
                     /locus_tag="WIE35_001334"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ4154244.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            3878..4315
                     /locus_tag="WIE35_001335"
     CDS             3878..4315
                     /locus_tag="WIE35_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ4154245.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            4384..4653
                     /locus_tag="WIE35_001336"
     CDS             4384..4653
                     /locus_tag="WIE35_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ4154246.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            4653..6428
                     /gene="ptsP"
                     /locus_tag="WIE35_001337"
     CDS             4653..6428
                     /gene="ptsP"
                     /locus_tag="WIE35_001337"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ4154247.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLENEIAKIVRCEDE
                     EKAEKLIKQINSVSVEEEADFKGRK"
BASE COUNT         1625 a         1764 c         1922 g         1317 t
ORIGIN      
        1 ctcggacagg aacggaaggc gtttgccgac caatatgcct tggaacgcca aatccgccaa
       61 agaatcgaaa ccgatttgga agaaagccgc caaactgtcc gcgacgtgca aaacgacctt
      121 tccgatgtcg gcaaccgttt tgccgcagcc gaaaaacaga ttgcccattt gcaggaaaaa
      181 gaggcggaag cggagcggtt gaggcagtcg cataccgagt tgcaggaaaa ggcacagggt
      241 ttggcggttg aaaacgaacg tttggcaacg caaatcgaac aggaacgcct tgcttctgaa
      301 gagaagctgt ccttgctggg cgaggcgcgc aaaagtttga gcgatcagtt tcaaaatctt
      361 gccaacacga ttttggaaga aaaaagccgc cgttttaccg agcagaaccg cgagcagctc
      421 catcaggttt tgaacccgtt gaacgaacgc atccacggtt tcggcgagtt ggtcaagcaa
      481 acctatgata aagaatcgcg cgagcgactg acgttggaaa acgaattgaa acggcttcag
      541 gggctgaacg cgcagctgca cagcgaggca aaggcactga ccaacgcgct gaccggtacg
      601 cagaacaagg ttcagggtaa ttggggcgag atgattctgg aaacggtttt ggaaaattcc
      661 ggccttcaga aaggacggga atatgtggtg caggcggcgg ctgtccgcaa agaagaagac
      721 ggcggcacgc gccgcctgca gcccgacgtt ttggtcaacc tgcccgacaa caagcagatt
      781 gtgattgatt ccaaggtttc gctgacggct tatgtgcgct atacgcaggc ggcggatgcg
      841 gatacggcgg cgcgcgaact ggcggcacac gttgccagca tacgcgcgca tatgaaaggc
      901 ttgtcgctga aggattacac cgatttggaa ggtgtgaaca ctttggattt cgtctttatg
      961 tttatccccg tcgaaccggc ctacctgttg gcgttgcaga atgacgcggg cttgttccaa
     1021 gagtgtttcg acaaacggat tatgctggtc ggccccagta cgctgctggc gactttgcgg
     1081 acggtggcga atatttggcg caacgaacag caaaatcaga acgcactggc gattgcggac
     1141 gaaggcggca agctgtatga caagtttgtc ggcttcgtac agacgctcga aagcgtcggc
     1201 aaaggcatcg atcaggcgca aagcagcttt cagacggcat tcaagcagct tgccgaggga
     1261 cgcgggaatc tggttggccg cgccgagaaa ctgcgtctgt tgggtgtgaa ggcaaacaag
     1321 cagcttgcac gcgatttgac cgaacatgcc aatgaaacaa cggcgttgtc ggaatctttg
     1381 gaatatgcgg cagaagatga agcagtctga cttgtgcgga aaaatattgt ttcagccgga
     1441 gcgggaatgc cgaaagcgtg gcgtaactgt accggttctg atttggggtt tgtttgaagt
     1501 acttaccaaa gccttgtctg gcgaggtaca ccggcaaggc agcggttgtc tgagcctttt
     1561 gtgtttcaga cggcatcagt gcagatgatg ccgtctgaag accgtagtga aagcggttag
     1621 aaaaacggat tgtgccgttt ttcgtgtccg atggaagtca tacgcccgtg tccggcgaca
     1681 acttgcacgg tttcggggag ggcgaataat ttgttgcgga tattattgat taagtcggcg
     1741 tggttgccgc gcggaaaatc ggttctgcct atggtttcgt aaaacagcac gtcgcccgca
     1801 atcagcaatt ccgcctcggc gcaataaaag acgacatgtc ccggcgtatg gcccggaata
     1861 tgcagcactt gaaaggcata gcgtccgacc gtgagcgttt cgccttcttc gagccaacgg
     1921 ttcggcgaaa atgcgggcga gacgggaaat ccgtattgcg cggtagtttg cggcagcgat
     1981 tggagcagga attcatcgtc cggatgcggc ccgaggacgg gaactttacg cgttttcagc
     2041 atttcgacca cgccgcctgc atgatcgaga tgaccgtgcg tcagccagat tgccgtgagc
     2101 gtaagtttgc ggtttgccaa cgcttgcagc aggaacggca cgtcgccgcc gacatcggtc
     2161 aggacggctt cgccgctttc gtcgtcccaa atcagggtgc agttttggcg gaagggggta
     2221 acggggaaga tttcgtaacg tagggtcatc ggcgtgttcc taaacggttt ttcagacggc
     2281 atcgggtttg ccgtttgttt tatggcggtt tgccgcccgt tttgatattg ggggaatcag
     2341 atgattaaga agacaatcgg cggcatcata ccgattttta cggcggtttt catccctgca
     2401 tcggcaggcg cggcggattt gatgctggcg caggaataca aagggcagga tattgccggc
     2461 tgggcaatga gcgagaaact cgacggcgtg cgcgcctatt gggacggaaa gcacctgata
     2521 agccgtcagg gctacgcgtt tacgcccccc aaaggcttta ccgcgcagtt tccgccttat
     2581 cctttggacg gcgaattgta tagcggacgt ggtcagttcg agcagatttc cgctaccgtg
     2641 cgttctgttt cttcagactg gcgcggcatc cgcctgcacg ttttcgatgt acccaaggcg
     2701 cagggcaatc tctaccaacg tttggcagtc gcaacgcagt ggctgaaaac gcatccgaac
     2761 gcgccgatta ccatcatccc gcaaaccaaa gtgcgcgacc ggcagcacgc gatggacttt
     2821 ttaaaacaaa tcgaagcgca gggcggcgaa ggcgtgatgc tgcgtcagcc cgaatcccgt
     2881 tacagcggcg gcaggagcag ccaattattg aagctgaaaa gccaatacga cgacgaatgc
     2941 acggtaacgc ggcactatga gggcaaaggg cgaaacgccg gacggttggg cgcgatcggc
     3001 tgcaaaaacc ggcacggcga attccgcatc ggcagcggtt tcaaagacaa agaccgcgac
     3061 aacccgccca aaatcggcac actgattacc taccgctacc gtggttttac gcggaaaggc
     3121 acgccgaaat ttgccacatt tgtgcgcgtg cgtaccgacc gctgagcagg acggttcaga
     3181 cggcatccga tactttggtt tataattttc cttttaccga ccgattccga catatgaccg
     3241 atttagaaac caaacgcctt gaaacacagg cgatgcttga aaacgccgat cttttgttcg
     3301 accaaggcca atgccgtgcc gcactgcaaa aagtggcgga cgagattacg cgcgatttgg
     3361 gcggcaaata tccgctgctg ctgcccgtga tgggcggcgc ggtggtgttt acggggcagt
     3421 tgctgccgct gttgcgtttt cccttagatt ttgattatgt tcacgtttcc cgttacggcg
     3481 acaagctgga gggcggcgcg ttcaactgga agcgtatgcc cgatgcggaa caaatccggg
     3541 gcaggcacgt cgtcgtgctg gacgatattt tggacgaagg gcatacgatg tccgccattc
     3601 aagccaaact tttggaaatg ggtgcggcaa gctgccgtgc ggcggtgttc gccaacaagc
     3661 tgattgacaa acccaagcct atccgagccg attatgtcgg actggatgtg ccgaaccgtt
     3721 atgttttcgg ttacggcatg gatgcggcgg gctgctggcg caatttgggc gagatttacg
     3781 cattggacgg aaaataagga cgcgatgccg tctgaaggct gttcagacgg cattgcgccc
     3841 atacgccggc aggacaacga ggaacaggac gcataatatg atagggcttt taatcatcac
     3901 acacgaaacc ataggcgaag cctaccgcaa gctggcacat catttttttc cgggcggact
     3961 gcctgaaaac gtccgcatac tcggcgtgca gccgacggaa gaccaagacg acatcatcaa
     4021 caacgccatt gccgcgcttc aggagtttcc cgacaacgac ggcgtgctaa ttatgaccga
     4081 tattttcggc gcgaccccct gcaatgcagc ccgccgcctc gtgcgcgaaa acaaatcggc
     4141 gattttgacc gggctgaacg cgccgatgat ggttaaggcc gtccaatatt cgccggcggc
     4201 ggaagacctt gccgccttta ccgaatgcgt cagggaggcg gctgtaaaag gcattttcgc
     4261 catcacgtcc gcgcccgaag atttggtgtg ccggcgcagc ggcgatgccg tctgaagaag
     4321 cggcagggca ggaaaacatt ttaaacggcg tctgctgccg atatacataa cacgggaacc
     4381 gaaatgctca aacaatccat cgaaatcatc aacaaactcg gactccacgc ccgcgcgtcc
     4441 aacaagttca cccaaaccgc gtcccaattc aaaagcgaag tctgggttac gaaaaacgac
     4501 agccgcgtca acggcaaaag cattatgggg ctgatgatgc tcgccgccgc caagggtacg
     4561 gtcatcgaac tggagacgga cggcgcggac gaggcggaag cgatgcgcgc cctgaccgac
     4621 ttaatcaacg gctacttcgg cgagggcgaa taatgagtat cgtgctgcac ggcgtggcgg
     4681 cgggcaaagg cattgccgtc ggttgcgccc acctgattgc gcgcggtacg gaggaagtgc
     4741 cgcagtatga tgttgcggag gcggacaccg atgccgaagc cgaacgtttc gatgccgccg
     4801 tcaaagccac gcgcaaagag ttggaacagc tccgcagcgc gattcccgaa aacgccccga
     4861 ccgagttggg cgcgttcatc tcgctgcacc tgatgctctt gaccgatgtt accttgtcgc
     4921 gcgaacccgt cgatatttta agggaacaaa aaatcaacgc cgagtgggca ttgaagcagc
     4981 agagcgacaa actcgccgcc caattcgaca atatggacga tgcctatttg cgcgaacgca
     5041 agcaggatat gctgcaagtc gtccgccgca tccacaacaa cctgatcggg cagggcaacg
     5101 agttggaagt tgccgacaac ctgtttgacg aaaccgttct gattgcaaac gacctttcgc
     5161 ccgccgacac ggttttgttt aaagagcagc gcattgccgc cttcgttacc gatgccggcg
     5221 gccccaccgg gcatacggcg attttgggca ggagcttgga catcccgtcc gtcgtcgggc
     5281 tgcacaacgc gcgcaaactg attaccgaag gcgaaacggt cattgtggac ggtatcaacg
     5341 gcgtgttgat tatcgcgccg gatgagtcgg tgttgaacga ataccgccgc cgtgcccgcg
     5401 aataccgcag ccacaaacgc gatttgaaca agctcaaaaa aaccgccgcc gccaccgccg
     5461 acggggtctg catcgagctt gtgggcaata tagaatccgc cgaagacgtg aaaccgctgc
     5521 acaacctcgg cgcagacggc atcgggctgt tccgcagcga gtttctttac ctgaaccgcg
     5581 atacgatgcc gtctgaagac gagcagtacg aagtgtacag cgcgattgtc aaaaaaatga
     5641 aaggcaaaag cgtaacgata cggacagtcg atttgggtgt ggacaaaaac ccgcgctggt
     5701 tcgggaaaaa cagcacgccc aacggcagcc tcaaccccgc gctgggcatg accggcatcc
     5761 gcctgtgcct tgccgaaccg gtcatgttcc gcacccagat gcgcgccatc ctccgtgcgg
     5821 cggtacacgg ccccgtgcgg atgatgtggc cgatgattac ctcagtatcc gaagtgcgcc
     5881 agtgcctcat ccacctcgac accgcgcaac gccagcttgc cgaacgcggc gatgccttcg
     5941 gtaaagtcgg catcggctgt atgattgaaa ttccgtctgc cgcgctgacc gtcggcagta
     6001 ttttgaaact ggtcgatttc atctccgtcg gtaccaacga cctgattcaa tacatcttgt
     6061 ccgtcgatcg cggcgacgac agcgtcagcc acctctacca gcccggccat cccgccgtgc
     6121 tgaaaatgct gcaacacgtc atccgtaccg ccaaccgcat ggacaaagac gtatccgtat
     6181 gcggcgagat ggcgggcgat accgcgttta cccgcgtttt attgggtatg gggctgcgcc
     6241 gtttttccat gaaccccaac aacatcctgc ccgtcaaaaa catcattctg cacagcaatg
     6301 tcggacagct cgaaaacgag attgccaaaa tcgtccgttg cgaggacgag gaaaaggcgg
     6361 aaaagctgat caaacagatc aacagcgtgt ctgtcgagga agaagccgac ttcaaggggc
     6421 ggaaataaat acggcaggta aaaaatagaa atacttaaca atgcccgcaa tctgaaattt
     6481 tgccattctt gcaaaataga aaaccgaaac agaaacccaa aatcggccat tccctcaaaa
     6541 acagaaaacc aaaatcagaa acctaaaatc cgtcattccc gcgcaggcgg gaatctaggt
     6601 ctgtcggcac ggaaacttat cgggtaaa
//
LOCUS       DAWHXZ010000108         6625 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000108 DAWHXZ010000000
VERSION     DAWHXZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.108"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..760
                     /gene="atpB"
                     /locus_tag="WIE35_001338"
     CDS             <1..760
                     /gene="atpB"
                     /locus_tag="WIE35_001338"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ4154248.1"
                     /translation="FSFINLDAVFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEI
                     LFEFVDDMCKSIIHNEKSRKAVAPLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNH
                     HALLRVVPTADLNTTLALAVGVLLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFL
                     LNLVEFLSKTVSHGMRLFGNMYAGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIA
                     GLAWAIFHILVITLQAFIFMALAFVYIGQAHDAH"
     gene            817..1053
                     /gene="atpE"
                     /locus_tag="WIE35_001339"
     CDS             817..1053
                     /gene="atpE"
                     /locus_tag="WIE35_001339"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ4154249.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            1124..1594
                     /locus_tag="WIE35_001340"
     CDS             1124..1594
                     /locus_tag="WIE35_001340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ4154250.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            1599..2132
                     /locus_tag="WIE35_001341"
     CDS             1599..2132
                     /locus_tag="WIE35_001341"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ4154251.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            2143..3690
                     /locus_tag="WIE35_001342"
     CDS             2143..3690
                     /locus_tag="WIE35_001342"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ4154252.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTAFPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLVLAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            3715..4590
                     /gene="atpG"
                     /locus_tag="WIE35_001343"
     CDS             3715..4590
                     /gene="atpG"
                     /locus_tag="WIE35_001343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ4154253.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            4628..6025
                     /gene="atpD"
                     /locus_tag="WIE35_001344"
     CDS             4628..6025
                     /gene="atpD"
                     /locus_tag="WIE35_001344"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ4154254.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            6036..6458
                     /locus_tag="WIE35_001345"
     CDS             6036..6458
                     /locus_tag="WIE35_001345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ4154255.1"
                     /translation="MSIMQVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         1618 a         1502 c         1791 g         1714 t
ORIGIN      
        1 tttttcgttt attaaccttg atgccgtctt ttttgccgtc ctgttgggcg taatcggcag
       61 cttcctgttg tggcgcggtg cgaaaaaagc aacggcaggc gttcccggac gtttccaggc
      121 tgccgtggaa atcttgtttg agtttgtgga cgatatgtgt aagagcatca ttcacaacga
      181 aaagtctaga aaagccgtcg cgccgttggg tctgacgctg tttgtctgga tttttctgat
      241 gaacgcgatg gatatgctgc cggtcgattt gctgccgatg gtatggcagg gcattaccgg
      301 caaccatcac gccctgctgc gcgtcgtacc gactgccgat ttgaacacca ctttggcact
      361 cgctgtcggc gtgttgctga tttgtatcta ttacaacatc aaaatcaaag gattgggtgg
      421 ctggtttcac gagttgttca gcgcgccgtt cggtgcaaaa ctcgcacctg cgaactttct
      481 gttgaatttg gtcgagtttc tttctaaaac cgtatcccac ggtatgcggt tgttcggcaa
      541 tatgtatgcg ggcgaattgg tattcttgct gatagccttg cttggcggtg cttgggcggc
      601 ttccggcagc gtcgaagtca tggatccgat tctgtttgta ttccacatta ttgccggttt
      661 ggcatgggcg attttccata ttttggtgat taccctgcag gcgtttattt tcatggcgtt
      721 ggcgttcgtc tatatcggac aggcacatga tgcgcactga ttttcctaag tagtttgttt
      781 ttgtagttta acctttgttt ctttaaggag tttaaaatgg gtttgattgc tatcgcatgt
      841 ggtttgatcg ttgcattggg tgcattgggt gcctctatcg gtatcgcaat ggtcggttct
      901 aaatatttgg agtcttctgc ccgtcagccc gaactgatcg gtccgctgca aaccaaactg
      961 ttcctgattg ccggtttgat tgatgccgca ttcttgattg gtgtcgccat tgcactgctg
     1021 ttcgccttcg ttaacccgtt tgcaggtgca taatcaggac ggattcgtcc gttccgtaca
     1081 ggcgcagcct gtttgattaa ccccagtacg aaggttaagt aacgtgaata tcaatgcaac
     1141 attattcgct caaatcatcg tctttttcgg tttggtatgg tttaccatga aattcgtgtg
     1201 gccgccgatt gcaaaagctt tggatgagcg tgccgcaaaa gtcgccgaag gcttggctgc
     1261 cgccgagcgt ggtaaaagcg atttcgagca ggctgaaaaa aaggttgcag aactcttggc
     1321 agaagggcgt aatcaggttt ccgaaatggt tgccaacgcc gaaaaacgtg ccgccaaaat
     1381 tgtcgaagaa gccaaagaac aggcttcttc cgaggcggcg cgcattgcag ctcaggcaaa
     1441 ggccgatgtg gagcaggaat tgttccgcgc acgcgaatcc ctgcgcgagc aggttgccgt
     1501 gttggctgtc aaaggtgccg aatctatttt gcgcagcgaa gtcgatgctt ccaaacacgc
     1561 aaaactgctc gataccctga aacaggagct gtaatcttat ggcagagttc gcaacgattg
     1621 ccagacctta tgcaaaggca ttgttcggtc tggctcagga aaaaaaccaa attgagtctt
     1681 ggttgggcgg actggaaaaa cttgcggcgg ttgttcagga agggaaggtg gcttcattga
     1741 ttgaccgtcc tgaaacgaat gcttcagaaa aagcagatat cctcatcgat ttggtcggtt
     1801 tgaaagacaa ggagttgaaa aactttgtta tcgtcttggc cgggcagaaa cgtttgtcga
     1861 tattgccgga agtatatgct caatatcaag acttgacctt atcattcaac catatcaaat
     1921 ctgccgtcat ttacagtgcc tatccgttga ccgacaaaca ggtcggcgag ttggcgcaaa
     1981 tgctgaataa gcgtttcgac agcgagctga aaatctctgt cgaaatcgaa ccagagctga
     2041 ttggcggcat aaaagttgaa gtgggtgatc aggttttgga tttgtctgta caaggcaaat
     2101 tgagtgcctt gtacacgact atgacgaatt aggagagttt tcatgcagct taatcctgct
     2161 gaaattagcg atttgattaa agccaagatc gaaaatctgt ctgtaaatgc cgaagtgcgt
     2221 acccgtggta cggtgatttc ggtaactgac ggtatcgttc gcatccatgg tttgtcagat
     2281 gcaatgcaag gtgagatgct cgaattcccg ggtaacactt tcggcttggc gatgaacctg
     2341 gagcgcgact ccgtcggcgc cgtagtgttg ggcgagtacg aacatattaa agaaggcgac
     2401 acggttactt gtaccggtcg tatcttggaa gtgccggtcg gacgcgaact ggtcggacgc
     2461 gtcgttgatg cattgggtcg tccgattgac ggcaaaggcc cgattaatac aactttgacc
     2521 gcccctatcg aaaaaatcgc accgggcgtg attgcgcgca aatcggttga ccagccgatg
     2581 caaaccggtc tgaaggcgat tgactctatg gttcctgtcg gtcgcggtca gcgtgagttg
     2641 attattggcg accgtcagac aggtaaaacg gccgtagcat tggatgccat cgtcaaccaa
     2701 aaaggtacgg gcgttatctg tatctatgtc gctatcggtc aaaaagcatc ttctattgcc
     2761 aacgtggtcc gcaaattgga agagcatggc gcgatggaac acaccatcgt ggttgctgca
     2821 actgcatctg aagcggcggc attgcaatat atcgcacctt actccggttg tacgatgggt
     2881 gaattcttcc gcgaccgcgg cgaagatgcc ttgattgttt atgacgattt gtccaaacag
     2941 gctgtggctt accgtcaaat ttccctgctt ttgcgccgtc cgcccggccg cgaagcctat
     3001 cccggcgatg tgttctacct gcactcccgt ctgttggaac gtgcagcacg tgtcaatgaa
     3061 cacgaagtgg agaaattgac caacggcgaa gtaaaaggca aaaccggttc tctgaccgcg
     3121 tttccgatta tcgaaaccca agcgggcgac gtatctgctt tcgttccgac taacgtcatt
     3181 tcgattaccg acggtcagat tttcttggaa accgacctct tcaacgccgg tatccgtcct
     3241 gcaatcaatg ccggtatttc cgtatcccgc gtaggcggtg ctgcacaaac caaagtgatt
     3301 aaaaagctgg gtggcggtat ccgtttggtg ttggcgcaat atcgtgaatt ggcggctttc
     3361 tcgcaatttg catccgattt ggatgaagct acgcgcaaac aattggagca tggcgaagtc
     3421 gtaaccgaac tgatgaaaca gaaacagttc agtacgttga atacggctga aatggctttg
     3481 accctttggg caatcaacaa cggttcgtat tctgatgttc cggttgccaa agctttggct
     3541 tttgaatctg aatttttgag ctttgtccgt acccaacatc cggaagtttt ggaagccgtc
     3601 aatgcttcag gtgcaatgtc cgacgagagc gagaaaacgc ttgaagcagc catgaaatcc
     3661 ttcaaatctt cttacgccta tcaggcataa ggcttgaagt gaaaggagtc tgaaatggca
     3721 gtaggaaaag agattctcac caaaatccgc agtgttcaga atacccaaaa gatcactaaa
     3781 gcgatgcaga tggtgtcaac ctctaaaatg cggaagactc aggaacggat gcgtttggcg
     3841 cgtccgtatg ccgaaaaagt gcgtatggtg atgagccatc ttgcgcaaac caataccgat
     3901 catggtattc cgttactgga atctcatcgg gaaatcagac gtgtcggttt tattttgatt
     3961 acgtctgata agggtttgtg tggcggtttg aacgccaacg tgctgaaaaa gtttttggca
     4021 caagttcaag agtatcggaa tcaaggtatt gaagaggaaa tcgtatgcct tggcagtaaa
     4081 ggtctgatgg cgtgtcagag cattggtctg aatgtggttg ccagtgccgt aaatttgggc
     4141 gataccccaa aaatggaaat gctgctcgga cctttgacag aactcttcca acggtatgag
     4201 aaacatgaaa ttgacagaat ccatctggtg tattcgggtt ttgtcaatac catgcgtcaa
     4261 gaaccgagaa tggaagtatt gctgcctatc ggtgagaacg tgattggcga ttcagctccc
     4321 aaatcaccgt tcagctggga ataccgctac gaaccgactg cacttgcagt gttggaatat
     4381 ctggttcgcc gctatttaga gtctgtggtt tatcaggcgt tgagcgacaa tatggcatcc
     4441 gaacaggcag cccgcatggt tgccatgaaa gctgcaacag acaatgcagg caatgccatc
     4501 aaagagttgc gtttggtata taacaaatcg cgtcaagctg cgattaccac ggaattgtca
     4561 gaaattgtag caggtgcggc ggctgtctga tgccgtctga atcctgtaca gaaattagga
     4621 tacgataatg agccaaggca aaatcgtaca aattatcggt gcggttgttg acgtggaatt
     4681 tccacgcgac atgattccgc gcgtttacga cgctttgaaa ttagacgaaa acggtctgac
     4741 tttggaagtc caacagcttt tgggcgacgg cgtagtccgt accattgcga tgggcagctc
     4801 ggacggtttg aaacgcggca tgactgtgag caataccggt gcgcccatta ctgtgccggt
     4861 aggtaaaggt acattgggtc gtattgtcga cgtattgggt acgcctgttg acgaggcagg
     4921 tccgatcgat accgacaaga gtcgtgccat ccaccaagcc gctcctaagt ttgacgaact
     4981 gtcttccaca accgaattgc tcgaaacggg cattaaagtg attgacttgc tgtgtccgtt
     5041 tgccaaaggc ggtaaagtag gtctgttcgg cggtgccggt gtgggtaaaa ccgtgaacat
     5101 gatggaattg atcaacaaca tcgccaaagc gcacagcggc ttgtccgtgt tcgcaggcgt
     5161 gggtgagcgt acccgcgaag gtaacgactt ctaccacgag atgaaagatt ccaacgtatt
     5221 ggataaagtg gcaatggttt acggtcagat gaacgaacct ccgggcaacc gtttgcgcgt
     5281 cgcattgacc ggtttgacca tggcggaata cttccgtgac gaaaaagacg aaaacggcaa
     5341 aggccgcgac gtattgttct tcgtggacaa catctaccgt tacactctag ctggtaccga
     5401 agtatccgca ttgttgggcc gtatgccgtc tgcagtgggc taccaaccga cattggcaga
     5461 agaaatgggt cgtttgcagg agcgtattac ctctacccaa accggttcta ttacttctat
     5521 ccaagccgta tatgtacctg cggacgactt gaccgacccg tctccggcaa caacgttcgc
     5581 ccacttggac gcaaccgtcg tattgagccg cgatattgcc tctttgggta tttacccggc
     5641 agtcgatccg cttgattcca cttcgcgcca actggatccg atggtcttgg gtcaagagca
     5701 ctacgacgtg gcgcgcggcg tacagtccac cctgcagaaa tacaaagaat tgcgcgacat
     5761 catcgccatc ttgggtatgg acgaattgtc tgacgaagac aaactgaccg taatgcgtgc
     5821 gcgtaaaatc caacgcttcc tgtcccaacc gttccacgtt gccgaagtgt ttacaggttc
     5881 tccgggcaaa tatgtcgccc tgcgcgatac cattgccggc ttcaaagcca tcttgaacgg
     5941 cgaatacgat catctgcccg agcaggcatt ctatatggtc ggcagcattg aagaagcggt
     6001 tgagaaagcg aaaaccttaa actgaggagg tcggcatgag catcatgcaa gttgaggtgg
     6061 taagtagcga gcagaaaatc tattcaggcg aggcaacatt tatcgttgtt ccgactgtac
     6121 agggcgaact cggtatttat ccgcgacacg agccgattat gagtttggtg cggccggggg
     6181 ctttgcgttt gaccgttccg ggcgaggata aagaggtttt ggttgctgtt tccggcggtg
     6241 ttttggaagt acagcctgat aaagtaactg tcttggcgga tgttgccgtc cgcagtgcag
     6301 agatggatca ggcacgtgcg gaagaggcga aaaaagccgc agaagcgggc atttcccaag
     6361 ctaaagacga taaggctttg gcggaggcgc ataaagcatt ggctgctgcg attgcacagc
     6421 ttaaaacttt ggactatatc cgttcgcaca agaaataaac ttgtttggtt tggaaaagca
     6481 cgaccgtttg gtcgtgcttt ttattttccg gatttaaatt aaagactgtg tttggatatg
     6541 gcgttggcag gcatagcctg ctttaaggta ttcaataggg cagcctgccg cctaaccgaa
     6601 acatatagcg gattaacaaa aatca
//
LOCUS       DAWHXZ010000109         6534 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000109 DAWHXZ010000000
VERSION     DAWHXZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.109"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            95..1174
                     /locus_tag="WIE35_001346"
     CDS             95..1174
                     /locus_tag="WIE35_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ4154256.1"
                     /translation="MKYKALSLLPLAAALAACAGGGVAEPHVPVSIPTATPLTGEVKL
                     SSDNSKIENINTANTETHPPRRTRRSLYASPQNTSSGISIQQREVEKIYFGVKSPEKS
                     FIFQTPGGAQYALSSYADPIVPSYSSPDFKIPDRHAGQRLADGSRIFICCSDSGATSY
                     AEITKQDYMKFGAWIGPNGEIDLFAGGFPVGKTPPPAFSYGNSTPETALSKGKITYQV
                     WGIRVKDGQFVTSSYTPPKGSSFTGYTNTPVLSFITANFNSNKLAGEIRGNSDYGPSV
                     KIENATISGPSFSGNATSGGKTGNLEGKFFGKFNGSYGNTETSIGGKITFKDDRSLDT
                     VFGGVSYVKKLDETANRDTEHLTKQ"
     gene            1202..3628
                     /gene="hupB"
                     /locus_tag="WIE35_001347"
     CDS             1202..3628
                     /gene="hupB"
                     /locus_tag="WIE35_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ4154257.1"
                     /translation="MSIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVKIENKGYVFSPTQSFSKYQTYVATGVARSQPDPQEWVNKSTLFKLGY
                     NFNDRNRIGWIFEDSRTDRFTNELSNLWTGTIYSPATGDYRHRQDVSYRRRSGVEYKN
                     ELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINSEVYHSFRHIRQNTAQWTAD
                     FEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFVKLYDPKIFTSNQAKITMLIESRSK
                     YKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAHAG
                     FSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAETAKNF
                     ELGLAGSGKAGNFKFSGFQTRYRNFIELTYMGVSSDKPNSPNYAPISDGTALVSSPVW
                     QNQNRSSAWVKGLEFNGTWNLDSIGLPQGTHAGVNVSYIKGKAKQTNGQETPINALSP
                     WSAVYNLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLFDL
                     SAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSPGR
                     SYNFTIEAKF"
     gene            complement(4236..5870)
                     /gene="groL"
                     /locus_tag="WIE35_001348"
     CDS             complement(4236..5870)
                     /gene="groL"
                     /locus_tag="WIE35_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ4154258.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGTVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAIESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(5964..6251)
                     /locus_tag="WIE35_001349"
     CDS             complement(5964..6251)
                     /locus_tag="WIE35_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ4154259.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVE"
     gene            6464..>6534
                     /locus_tag="WIE35_001350"
     CDS             6464..>6534
                     /locus_tag="WIE35_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ4154260.1"
                     /translation="MLPSKYEDNALQIKEKSTERAAP"
BASE COUNT         1607 a         1915 c         1553 g         1459 t
ORIGIN      
        1 tttttgcacc gacccacaaa accagttgac atcatataac atttaaactg ataatcaaaa
       61 ctattattaa cacatcatca agattgagta tgcgatgaaa tacaaagccc tctccttact
      121 gccgcttgct gccgcccttg ccgcctgtgc cggggggggg gtagccgaac cgcacgtccc
      181 cgtgtccatc cccaccgcca cgccgctgac cggtgaggta aagttatcaa gcgataacag
      241 caaaatcgaa aacatcaaca ccgccaacac tgaaacacat ccgccgcgcc gcaccagacg
      301 ctccctctat gcctccccgc aaaacacatc ctccggtatt tccattcagc agcgggaagt
      361 agaaaaaatc tatttcggtg ttaaatcacc agaaaaatca ttcatcttcc aaacacccgg
      421 cggcgcgcaa tacgcccttt catcctacgc cgaccccatc gtccccagct actcctcccc
      481 cgatttcaaa attcccgacc gccacgcagg gcagcggctt gccgacggca gccgcatctt
      541 tatctgctgc agcgactccg gggctacttc gtatgcggaa attaccaaac aagattatat
      601 gaaattcggg gcgtggatag gtcccaacgg cgaaatcgac ctcttcgccg gcggcttccc
      661 cgtcggcaaa acgccacctc ctgcgttctc ctacggcaac agcacgcccg aaaccgcatt
      721 gtcgaagggc aaaatcactt atcaggtttg gggcatccgc gtcaaagacg ggcaatttgt
      781 tacctcctct tatacgccgc ccaaaggcag ctcttttacc ggctacacca acacccccgt
      841 cctttccttt atcaccgcca atttcaacag caacaaactg gcaggcgaaa tccgcggcaa
      901 cagcgattac ggaccgagtg tgaaaattga aaacgcaacg atcagcggtc cgagcttttc
      961 cggcaatgcc acctccggcg gcaaaaccgg caacttggaa ggcaagtttt ttggaaaatt
     1021 caacggcagc tacggcaaca ctgaaaccag cataggcggc aaaatcactt ttaaagacga
     1081 ccgctccctc gataccgtat tcggcggtgt gagttatgta aaaaaacttg atgaaaccgc
     1141 caatcgagat accgaacacc ttactaaaca ataaaccatc tcatccctac aaaggtttct
     1201 tatgtccatc cccttcaaac ccgtattggc tgccgccgcc atcgcccaag cgtttcccgc
     1261 ctttgcggca gaccccgcgc cgcagtccgc ccaaacgctg aacgaaatca ccgttaccgg
     1321 cacgcacaaa acccaaaaac tcggcgaaga aaaaatccgc cgcaaaacct tagacaagct
     1381 cttggtcaac gacgaacacg acctggtgcg ctacgacccc ggcatttccg tcgtcgaagg
     1441 cggcagggcg ggttctaacg gctttaccat acgcggcgtg gacaaagacc gcgtcgccat
     1501 caacgttgac gggctggcgc aggcggaaag ccgctcttcc gaagccttcc aagaattgtt
     1561 cggcgcgtac ggcaacttca acgccaaccg caacacttcc gagccggaaa acttttccga
     1621 agtaaccatc accaaaggcg cggactcgct caaatccggc agcggcgcat tgggcggcgc
     1681 agtcaattac caaaccaaat ccgcaagcga ttatgtttcc gaagacaagc cctaccattt
     1741 ggggataaag ggcggcagcg tcggcaaaaa cagccaaaag ttcagcagca tcaccgccgc
     1801 cggcaggctc tttggtttgg atgccttatt ggtttatacc cgccgcttcg gcaaagaaac
     1861 caaaaaccgc tcgaccgagg gcgatgtcaa aattgaaaac aagggatatg ttttcagccc
     1921 aacccaatca ttcagcaagt accagaccta cgtagccaca ggggttgcgc gctcccaacc
     1981 cgacccgcaa gaatgggtaa acaaaagcac cttgttcaag ctgggctaca atttcaacga
     2041 ccgcaaccgc atcggctgga tttttgaaga ctcccgcacc gaccgtttta ccaacgagct
     2101 gtctaatttg tggacgggta caatttactc tcccgcaacg ggcgactacc gccaccgcca
     2161 agacgtgagc taccgccgcc gctccggcgt cgaatacaaa aacgaattgg aacacggccc
     2221 gtgggacagc ctcaagctgc gctacgacaa gcagcgcatc gatatgaaca cttggacttg
     2281 ggacatcccg aaaaattacg atttgcgcgg catcaacagt gaagtttacc attcgttccg
     2341 gcatatccgc caaaacaccg cgcaatggac tgccgatttt gaaaaacaac tcgacttttc
     2401 caaagccgtt tgggcggcgc aatacggctt gggcggcggc agaggggaca atgccaactc
     2461 ggattacagc tatttcgtaa aactgtacga ccccaaaatc ttcacttcca accaagccaa
     2521 aatcacgatg ctgatcgaaa gccggtcgaa atataaattt gcctattgga acaatgcgtt
     2581 tcacttgggc ggcaacgacc gcttccgcct gaatgcgggc atacgctacg acaaaaacag
     2641 cagcagcgcg aaagacgatc cgaaatacac caccgccatc cgggggcaga ttccccattt
     2701 gggttcggaa cgcgcgcacg cgggcttcag ctacggcacg gggttcgact ggcggtttac
     2761 caagcatctg cacttgttgg caaaatacag caccggcttc cgcgcaccga cttcggacga
     2821 aacctggcta ttgttcccgc atcctgactt ttaccttaaa gcaaacccca acctgaaggc
     2881 agaaaccgcc aaaaacttcg agctgggact tgccggcagc ggcaaagcag gaaactttaa
     2941 attctccggt ttccaaaccc gctaccgtaa ttttatcgaa ctgacctaca tgggcgtatc
     3001 ctcagacaag cctaacagcc ctaattatgc gccgatttcc gacggtactg cattggtcag
     3061 ctcgcccgtt tggcaaaacc aaaaccgctc ctcggcatgg gtgaaaggtt tggaatttaa
     3121 cggcacatgg aacctcgaca gcatcggttt gccgcaaggc acgcacgcag gtgtcaatgt
     3181 cagctacatc aaaggcaagg ccaaacaaac caacggacag gaaaccccta ttaacgccct
     3241 ttcgccatgg agtgcggttt acaatctggg ctacgacgct ccttccaaac gttggggcat
     3301 caacgcctac gccacgcgca ccgccgccaa aaagccgtcc gacaccgtcc acagcaacga
     3361 cgacttgaac aacccgtggc cttatgccaa acacagcaag gcctatacgc tgttcgacct
     3421 ttccgcctac ctcaacatcg gcaaacaggt tacgctccgc gccgccgcgt acaacattac
     3481 caacaagcag tactacactt gggaatcttt acgcagcatc cgcgagttcg gcacggtcaa
     3541 ccgcgttgac aacaaaaccc acgccggcat ccaacgcttt acctcgccgg gcaggagcta
     3601 caatttcacc atcgaagcga agttctaagc tcctgatgtg ccaatgccgt ctgaaacccg
     3661 atgatttggg tttcagacgg cattttttac ggtcgcaccg ccgccccctt tcaggcttgt
     3721 accaagccca atcccgcaac tctccgtcat tcccgcaact ctccgtcatt cccgcgactc
     3781 tccgtcattc ccacggaagt gggaatctag aaacgcaaag ctgcaagaat ttatcggaaa
     3841 tgactgaaac tcaacgaacc tggattcccg cctgcgcggg aatgacgaag ctatccgcac
     3901 agaaacctgc accacgtcat tcccacgaaa gtgggaatcc agaacgtaaa atctgaagaa
     3961 accgttttat ccgatacgtt tccgcaccga cagacctaga ttcccgcttt cgcgggaatg
     4021 acggcggaaa ggttgctgtt tttccgataa attcctgccg ctcttcgttt ttgggatggc
     4081 gggaaataaa acaaaagcgc gcgtatcaaa aaacaaaaat gcaaagaacg gcgttggcaa
     4141 ttttaaggtt tgcgccttag ccgcacttat tcgcaaaaaa aaccgcacgg cgttgaccgt
     4201 gcggcttgtt gtctgaaagc ttcagacggc atttcttaca tcatgccgcc cataccgccc
     4261 atgccgccca tgtcaggcat agcaggtttt tcttcaggga tttcagcgat catgcagtct
     4321 gtggtcagca tcagaccggc gatagatgcg gcgtgttgca gcgcggaacg ggttactttg
     4381 gcaggatcga gtacgcccat ttcaatcata tcgccgtatt cgccgctgcc agcgttgtaa
     4441 ccgtagttgc ctttgccttc caacactttg ttcacgacta cgctgggttc gccgcctgcg
     4501 ttggcaacga tttggcgcag cggagactca atggcgcgca agacgatttg tacgcctgcg
     4561 tcttgatcgg cattgccggt gtgcaggttt tccagagcgg cacgggcgcg caacagggct
     4621 acgccgccgc ctgcaaccac gccttcttca acggctgcgc gggtagcatg cagcgcgtct
     4681 tctacgcggt cttttttctc tttcatttcg acttcggtag cggcaccgac tttgattact
     4741 gccacgccgc ctgccagttt ggccacgcgc tcttgcagtt tttctttgtc gtaatcgctg
     4801 gttgcggttt cgatttgttg gcggatttcg gcaacacgcg cttcgatttg ggctgcgtcg
     4861 ccgaagccgt cgatgatggt ggtgttttct ttaccgattt cgatgcgttt ggcttgaccc
     4921 aagtcgtcca aagtcgcttt ttccagagac aggccgactt cttcggaaat caccgtgccg
     4981 ccggtcagga tggcgatgtc ttgcagcatc gctttgcggc ggtcgccaaa gcccggagct
     5041 ttaacggcaa cggttttcag gatgccgcga atgttgttta ccaccaaagt cgccaaggct
     5101 tcgccttcta cgtcttcagc gataatcaag agcggacggc tggcttttgc cacttgttcc
     5161 aaaacaggca acaagtcgcg gatgttgctg atttttttgt cgaacagcaa tacaaacgga
     5221 ttgtccaagc cggcgatttg tttttccgca tcgttgatga agtaaggaga caggtagccg
     5281 cggtcgaatt gcataccttc aaccacgtcc agctcgtttt ccaaagattt gccgtcttca
     5341 acggtaatca cgccttcttt gccgactttt tccatcgctt cggcaataat cgcgccgact
     5401 tgttcgtcgg agttggcgga aatcgagccg acttgggcga tttctttaga agtgtcgcaa
     5461 ggtttggcga tgtttttcag ctcttcaacc aaagcggcga cggctttgtc gataccgcgt
     5521 ttcaggtcgg tcgggttcat accggcggta acgtatttca taccttcggc aacgatggat
     5581 tgcgccagta cggtggcggt agtcgtaccg tcgcccgcca cgtcgttggt tttggacgcg
     5641 acttctttta ccatttgcgc gcccatattt tcaaacttgt ctttcagttc gatttcttta
     5701 gcgacggtta cgccgtcttt ggtaatgtgc gggccgccga aagcgcggtc aaccaccacg
     5761 ttgcggcctt tgggacccag ggttacgcgc acggcgtttg ccagaatgtt cacgccgttt
     5821 accatttttt gacggacttc attgccgaat tgtacgtctt ttgctgccat ttcaattctc
     5881 caaaaatcat taaaactgtc tgataaaacc gtttatgccg tctgaagacg gtttgccgtt
     5941 tcagacggca tcatcattcg cacttattca acgatgccga aaatatcttc ttcgcgcatt
     6001 accaacagct cttcgccgtc ggcttttacg gtttggccgc tgtatttgcc gaaaatgatt
     6061 ttgtcgccga ctttgacatc cagcggacgg cgcgcgccgt ctttaccgat tttgcccgcg
     6121 cccacggcga tgacttcgcc catatcgggt ttttcggcgg ccgcacccgg caaaacgatg
     6181 cccgatgcgg ttttctcttc agcttccaag cgtttgacga cgacgcggtc gtgtaaagga
     6241 cggatggtca tatctatgct ccgataaaat ggtttgaaaa caatcatctg cccgaacggt
     6301 tcggacagat taaggatgaa aaccggacag ccgtcaggcg gctgcccgta taagtcggca
     6361 aaattagggt gtgtgcgggc aaattcaagt gaggcggaaa aaatttattt ccgccgtttt
     6421 ttatagtgga ctaaatttaa ggggctgtac tagattagca gatatgttac cctcgaaata
     6481 tgaagataac gcactgcaaa ttaaagaaaa aagtacagaa agagctgctc cgtt
//
LOCUS       DAWHXZ010000110         6513 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000110 DAWHXZ010000000
VERSION     DAWHXZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.110"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(19..504)
                     /gene="purE"
                     /locus_tag="WIE35_001351"
     CDS             complement(19..504)
                     /gene="purE"
                     /locus_tag="WIE35_001351"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ4154261.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLNMELEQ
                     I"
     gene            complement(552..1268)
                     /locus_tag="WIE35_001352"
     CDS             complement(552..1268)
                     /locus_tag="WIE35_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ4154262.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            complement(1268..1936)
                     /locus_tag="WIE35_001353"
     CDS             complement(1268..1936)
                     /locus_tag="WIE35_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ4154263.1"
                     /translation="MTTHLSNVAPDLQNYLNSIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPRYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(1947..2132)
                     /locus_tag="WIE35_001354"
     CDS             complement(1947..2132)
                     /locus_tag="WIE35_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ4154264.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            2275..4251
                     /gene="mutL"
                     /locus_tag="WIE35_001355"
     CDS             2275..4251
                     /gene="mutL"
                     /locus_tag="WIE35_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ4154265.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPEQSLHERIAAIVGDDFQT
                     ASLEIDSGSGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVVSTPMPSENDSENLFDRASNYPTGNKSDTHNAFGSSGKTAPMP
                     YQSAYAPQQRSLSLRESRAAINTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGRLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAAPAML
                     GKSDVVSLARDVLGELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            4346..6469
                     /gene="dnaX"
                     /locus_tag="WIE35_001356"
     CDS             4346..6469
                     /gene="dnaX"
                     /locus_tag="WIE35_001356"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ4154266.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYAPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAEQDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAREMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTIGGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEAETAQTPVQTASAAAMPSESKTAEPVTNQENNDVPPWEDAPDEAQTAAGTT
                     AQASAKSIQTASEAETSPDNQVSKNKAADNETETSLSEVPSENPIQATPNDEAVETEA
                     FAHEAPAEPFYGYGFPDNDCPPEDGAEIPPPDWEHAAPADAEEENNADEGSNDENNTP
                     SAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARATAD
                     KKRLDKIRDTLAQAYGLQLTLQTQDWHDESGRETPAMQDKRVQAEDRQKAQALLEADP
                     AAQKILQAFGAQWQPESLELAANRP"
     misc_feature    5535..5574
                     /gene="dnaX"
                     /locus_tag="WIE35_001356"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
BASE COUNT         1554 a         2028 c         1666 g         1265 t
ORIGIN      
        1 attcgcagac ggtgggtttt aaatttgttc caattccata ttcaaaacag cctgttcctg
       61 tttggctcgg aagtctgcca gtttttgcgc cagttcgggg gtttcgttgg cgagcatgga
      121 aacggcgaac aatgcggcat ttgccgcgcc tgcctcgccg atggcgaatg tggcgacggg
      181 tacgcctttg ggcatttgta caatcgataa aagcgaatct tcgccgcgca ggtatttgct
      241 ggggacgggt acgcccaaaa cggggacggt ggtcttggcg gcaaccatac cgggtaaatg
      301 cgccgcgccg cccgcacccg cgatgatggc tttgatgccg cgcgcccgtg cggtttcggc
      361 gtattggaac atcaaatccg gggtgcggtg tgcggaaaca acgcgcgcct catattctac
      421 gccgaactct tcaagaaact gcgctgcctg ccgcataacg ggccaatcgc tgttgctgcc
      481 catgatgatg ccgatttgta tcataaatcc tccttggtgc ggatggggta aaaagcggaa
      541 aaatggaaaa actatcgttt gcgcacggct gcggcggcgc gttttgccgc cgggctgctc
      601 gggtaagcct gtatcaggct gcgccaagtc gcccttgcaa tgtctttctg ctgcaacctg
      661 tattggcatt cgccgatttt gaacatggct tcgggcgcgg ttgggctgtc tttgaaacgg
      721 ttggcgtaac gccctccgat ttcgatgacg gattcgcagt tgcccatacg cgccctgctt
      781 tgcagcaaca gatacatact gcgttgcgcg atgctgccgc cgtcgcctcc gtccgcgcct
      841 ttcaacaggg aggcagcggc agaaaacctg ccgcttttat agtgtttgag tgcctgattg
      901 tagaggtttt gtgcggtttc gacggtatgt gcggatgcgc tgccgccttc ggtattgagg
      961 taatgctctt tcaacttgcg gtcgtcgagt ttttggacgt atgccctgct ggaagggtgt
     1021 gttttcgcat gctccagtgc tttgactttg ccgtttaagg tttccacttc gttcgacagg
     1081 cggacgagtg tgccttccag ataatccaaa cggtcttgca gagtgggaac gggataggga
     1141 atagcgtccg aactacctgc ctctgcctgc ggttcgggcc gcatatcctg aatattgcgg
     1201 gaaacagggg aagaacaggc ggcagatacg gacagccaaa tgataaaaag cggtaatttg
     1261 gtcttcatta ttttttcaga agcagggtca agccgtcgcc gacgggcagg gtgatgggga
     1321 caatccgcgt atcgttcggc aggttttgat tgaaatcttt gaggatgccg acgctgggcg
     1381 gcgcatcgga agccgcttcg cgcatcaccc ttccgttcag caaaatattg tcgatggcga
     1441 tgatgccgcc ttgacggaca agcttgaggc aacgctcgaa atatcgcggc gtgggcggtt
     1501 tgtctgcgtc tatcagtgcc aaatcgtagc ttccggcttc accctgtgca atcaaatcat
     1561 ccaatgtcag caatgcgggt tgcaggtgca ggctgatttt atgtgccaca cctgcctcgt
     1621 tccaaacctg acgcgccgta tcggtaaagg ttacattgat gtcgcaggcg gtaatccgcc
     1681 cgtgttctgg caacgccaat gcaagcgcgg tgctgctgta tccggtaaac acgccgactt
     1741 ccagatattt ttccgcgcga atcagctttg ccagccaaac caaaaccgcc gcctgttcgc
     1801 gcgcaatcgc cattttgccc atacggtgat gctcggtctt ctcacgcagc cgcgtcaaaa
     1861 cgggatgttc gggttcgccg atgctgttca gatagttttg caggtccggt gcgacattgg
     1921 acagatgggt cgtcatttcg gcgggttcag tcttggtaat aggtataagg ttttttcgct
     1981 acttttgccg cctcgaagtt ttcctgttct tcgggattga gttcgacatc ccacaaaagc
     2041 ccccggtttt ccaaacgctg ctgttccaac tcaggttttt cttcaatcag gcggttgagg
     2101 aattgtgtgg cttcggattg gtagtgatac atctttgcgc tccaatttta cgggatacgg
     2161 cgtgattata ctggtatttt ccaaacggga taaacggctt ttatcaagaa tacaggcaga
     2221 aaagtaaggg gttttattat agaataaagc gtttttgcaa cggaagccta ccttatgcca
     2281 cgcattgccg ccctgcccga ccatcttgtc aaccaaatcg ccgccggcga agtggtcgaa
     2341 cgccctgcca acgccttgaa agaaatcgtt gaaaacagta tcgatgcagg cgcaacggcg
     2401 attgatgtcg agctggcggg cggcggcatc cgcctgattc gcgtcagcga caacggcagc
     2461 ggtattcatc cggacgacat cgagcttgcg ctccaccgac acgctaccag caaaatcaaa
     2521 accttaaacg atttagaaca cgttgccagc atgggctttc gcggcgaagg cttggcaagc
     2581 atcgcctccg tcagccgcct gaccctgacc agccgtcaag aagacagttc gcacgcaacc
     2641 caagtcaaag ccgaagacgg caaactcagc agccccaccg ccgccgccca ccccgtcggc
     2701 accaccatcg aagccgccga actcttcttc aacacccccg cgcggcgcaa gttcctcaag
     2761 tctgaagcca ccgaatacgc ccactgcgcc actatgctcg aacgcctcgc gctggcgcat
     2821 ccgcacattg ccttctcgct caaacgcgac ggcaaacaag tgttcaaact ccctgaacaa
     2881 agcctgcacg aacggattgc cgccattgtc ggcgatgatt ttcagacggc atctttggaa
     2941 atcgacagcg gcagcggcgc attgcggctc tatggcgcga ttgccaagcc gactttcgcc
     3001 aaaggcaaaa ccgacaaaca atactgcttc gtcaaccatc gttttgtccg cgacaaagtg
     3061 atgctccacg ccgtcaagca ggcataccgc gacgttttgc acaacgccct cacccccgct
     3121 ttcgtccttt tcctcgacct gccgcccgaa gccgtggatg tcaacgtcca cccgaccaaa
     3181 accgaaatcc gcttccgcga cagccggcag gtgcaccaac tcgtgttcca cacgctcaat
     3241 aaagcccttg ccgacacacg cgccgacttg accgaaagcg tcagcaacgc aggcgaagtg
     3301 ttgcatgaca ttaccggcgt tgtctccacc ccaatgccgt ctgaaaacga cagcgaaaat
     3361 ctgtttgatc gcgcatccaa ctacccgaca ggcaacaaat cagatacaca caatgccttt
     3421 ggttcatcag gcaaaaccgc gcccatgccc tatcagtccg catatgcgcc gcaacaacgc
     3481 agcctgtccc tgcgcgaaag ccgcgcggca ataaacacct atgccgaact ctacaaaaaa
     3541 accgacgaca tcgaccttga gttaagccga ttcgagcagg cacgtttcgg caatatgccg
     3601 tctgaaacgc ctgctcccca aacagatacg ccgctttcag acggcatccc gtcccaatcc
     3661 gaactgccgc cgctcggttt tgccattgcc caattacttg gcatctacat tcttgcccaa
     3721 gccgaagaca gcctgttgct catcgatatg cacgctgccg ccgaacgcgt caactacgag
     3781 aaaatgaaac gccaacggca ggaaaacggc aggctgcaaa gccaacgcct gcttattccc
     3841 gtaacctttg ccgcgtccca cgaagaatgc gccgcccttg ccgattatgc cgaaacgctg
     3901 gcaggcttcg ggctggaatt atccgatatg ggcggcaaca ccctcgccgt ccgcgccgca
     3961 cccgcaatgt tgggcaaatc cgatgtcgtc tccctcgccc gcgacgtatt gggcgaactc
     4021 gcccaagtcg gcagcagcca aaccatcgaa gaacacgaaa accgcatcct cgccactatg
     4081 tcctgccacg gctcgatccg cgccggccgc cggctcaccc tgcccgaaat gaacgccctt
     4141 ctgcgcgata tggaaaatac gccgcgcagc aaccagtgca accacggcag gccgacttgg
     4201 gtcaaactga ctttgaaaga attggacgca ctgttcttgc gcggacagta agccgaaagt
     4261 gctagaatac gccgcccgag accgccgttc agacggcatt ccgacgcacc gacagaaaca
     4321 tcacgaccga aaccaagaga aaaacatggc ctatcaagtt ctcgcccgaa aatggcggcc
     4381 caaaaccttt tccgacctag tcggtcagga acacgtcgtc aaagccctgc aaaatgcctt
     4441 ggacgaaggc aggctgcacc acgcctacct gctgaccggc acgcgcggcg taggcaaaac
     4501 caccatcgcc cgcatccttg ccaaaagcct caactgcgaa aacgcgcaac acggcgaacc
     4561 ttgcggcgtg tgccaaagct gtacgcagat cgatgccgga cgctatgtcg acctgctgga
     4621 aatcgacgcc gcctccaaca caggcatcga caacatccgc gaagtattgg aaaacgccca
     4681 atacgcgccg accgccggca aatacaaagt ctatatcatc gacgaagtgc atatgctttc
     4741 caaaagcgcg ttcaacgcta tgctcaaaac gctggaagag ccgcccgaac acgtcaaatt
     4801 catccttgcc acgaccgatc cgcacaaagt ccccgttacc gtattaagcc gctgcctgca
     4861 attcgtctta cgcaatatga ccgcgcagca ggttgccgac caccttgccc acgtcctcga
     4921 cagcgaaaaa atcgcctacg ccccccccgc cctgcaactt ttgggacgcg ccgccgccgg
     4981 atcgatgcgc gatgccttga gcctgctcga ccaagccatc gccctgggtt cgggcaaagt
     5041 tgccgaacaa gatgtccgcc aaatgattgg tgcggttgac aaacaatacc tttacgaact
     5101 gctgacaggc atcatcaacc aagacggcgc agccctgacc gccaaagcgc gggaaatggc
     5161 ggcgtgtgcc gtcggctttg acaacgcctt gggcgaactc gccatactgc tgcaacacct
     5221 cgccctgata caggcagtgc cgaatgcctt ggcgcacgac gaccccgatt ccgatatttt
     5281 gcaccgcctc gcccaaacca taggtggcga acaaatccag ctttactacc aaatcgccgt
     5341 ccacggcaaa cgcgacttgg gcctcgcccc cgacgaatac gccggcttta tgatgaccct
     5401 gctgcgtatg ctggcgtttg cgcccttggc ggcagcttcg tgtgatgcaa acgccgtgat
     5461 tgaaaatacc gaacttcaat ccccatcggc acaaaccgcc gaaaaggaaa ccgccgcaaa
     5521 aaagccccaa ccgcgccctg aagcggaaac cgcccaaaca cccgttcaga cggcatccgc
     5581 agcggcaatg ccgtctgaaa gcaaaactgc cgaacccgtt accaatcaag aaaacaacga
     5641 tgttccgcct tgggaagacg cgccggacga agcccaaacc gcagccggca cgaccgcgca
     5701 agcatcggca aaaagcattc agacggcatc cgaagccgaa acatcgcctg acaatcaagt
     5761 ttccaagaac aaggcagccg acaacgaaac cgagacttcc ttgtccgaag tgccgtctga
     5821 aaaccccatt caggcaacac cgaatgatga agccgttgaa acggaagcat ttgcgcacga
     5881 agcccctgca gaacctttct acggttacgg ctttccggat aatgactgcc ccccggaaga
     5941 cggcgcagaa atcccgccgc cggattggga acacgccgcc cctgccgatg cggaagaaga
     6001 aaacaacgcc gacgaaggca gcaacgacga aaacaacacg ccgtccgccc cgccgcccga
     6061 gttttccacc gaaaactggg cagccatcgt ccggcatttc gcccgcaaac tcggcgcggc
     6121 gcaaatgccg gcgcaacact ccgcgtggac ggaataccat cccgacaccg gcctgatggt
     6181 tttggcaatg accgccgaag cacgcgccac cgccgacaaa aaacgcctcg acaaaatccg
     6241 cgacaccctt gcccaagcct acgggctgca actcaccctg caaacccaag actggcacga
     6301 cgaatccggc agagaaaccc ccgcaatgca ggacaagcgc gtccaagccg aagacaggca
     6361 aaaagcacaa gcattgctcg aagccgaccc cgccgcacaa aaaatcctcc aagcattcgg
     6421 cgcgcaatgg cagcccgaat cactggaatt ggcggcaaac cggccataaa cagatataat
     6481 gccgcccgaa cccttcggac ggcattgccg ttt
//
LOCUS       DAWHXZ010000111         6512 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000111 DAWHXZ010000000
VERSION     DAWHXZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.111"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(143..826)
                     /locus_tag="WIE35_001357"
     CDS             complement(143..826)
                     /locus_tag="WIE35_001357"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ4154267.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVIPDGENLQVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1122..3425
                     /gene="parC"
                     /locus_tag="WIE35_001358"
     CDS             1122..3425
                     /gene="parC"
                     /locus_tag="WIE35_001358"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ4154268.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKTDLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLSDENEKRKLIVKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTI
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATNQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            3445..4962
                     /locus_tag="WIE35_001359"
     CDS             3445..4962
                     /locus_tag="WIE35_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ4154269.1"
                     /translation="MVISNTRELEKLKDRIPNLINIARIAIVFPLLIMHIFGLEVSGH
                     ENLHASWTAWAFYVWLAISCWLIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVVCSCLLSYGRYPLLYASYAAILLIFNAIADGDIGKYPHISDAR
                     TASATFILVAASYLSAIFTSLSVRYIDSAGKLAHENHAAYRRIRGLNQIVLNRVQEAV
                     VVINVEHQTILFNKKAKDLLPMLEIGQHTALFDPITVLWDKASSRTFERNIDTPALTA
                     RIRAVPMNKEQNKLLILYIRPQSEIQAEALSVKLAALGQLTANLTHEIRNPMSAIRHA
                     NGLLRENMEAGAADPFNAKLCKIIDGNVCRINKMLEDISSLNKRNKTEREAIGLIPFW
                     EEFKQEFLLGHPDAAGCIRLDMQGNHLTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            5014..5481
                     /locus_tag="WIE35_001360"
     CDS             5014..5481
                     /locus_tag="WIE35_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ4154270.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            5585..6337
                     /locus_tag="WIE35_001361"
     CDS             5585..6337
                     /locus_tag="WIE35_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ4154271.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         1601 a         2016 c         1606 g         1289 t
ORIGIN      
        1 acctaaaacc catccttccc acgaaaacag aaaaacaaaa acctaaaatc ccgtcgttct
       61 cgcgaaagca gttgctacgt ttaccgtcaa aaatgccgtc tgaacgctca agccttcaga
      121 cggcattttt tatttcacac ccttacaggt agaatttttc gatgactttc aaattgtcgt
      181 ccaatttgta caccaacggc tgaccggtcg ggatttccaa gcccatgatg tcttcgtcgg
      241 aaatgccctc gatgtgtttt gccagcgcgc gcagggagtt gccgtgcgcc gccaccaaga
      301 cgcgtttgcc gctcaaaatc gcgggggcga tttggtcttc ccaaaacggc aatacgcgct
      361 ccagcgttac ttgcaggttt tcgccgtcgg ggataacatc ggcaggcaga tgggcatagc
      421 ggcggtcttt gtgtgcggaa aactcatcgt ctttgtccaa aagcggcggc agggtgtcgt
      481 agctgcgccg ccagatgcgg acttgctcgt cgccgtattg ttcggcggtt tgttttttgt
      541 ccaggccttg cagtcggccg tagtggcgtt cgttcagccg ccatgttttg atttgcggta
      601 cgaacagttg gtcggattct tccaaaacga tgttgcaggt cttaatcgcg cgggtcagga
      661 cggatgtgaa ggcgatgtcg aactcatagc cgttttcttt cagtttttta ccggcggcag
      721 cagcctcggc aagcccctgc tcgctcagct tcacgtcgcg ccagcctgta aacaggtttt
      781 tcgcgttcca ttcgctttgt ccgtggcgga taaataccaa ttccatatcg tctccaatgt
      841 gtgaaagtgg gaaagcctta tttataacat attttcacat tcccgtattt gattcagatt
      901 cagacacgcg cccactatgg tttgccgttt tgatttacaa taatgccctt tgctttacat
      961 tccgcataca caatgtttca acacacagga cgacacataa agcaccgccc tatgtgttgc
     1021 cctgatttgg aaggggttgc cccctcccaa ataaagtctg attctaccgc cccgaaggac
     1081 agatgtccaa gtggcggggg ttcaaccgaa aaggaaatac gataaatatg caaccgcacg
     1141 ctccacatac cgattccaat acgctgatgc tcggccgata cgccgaacgc gcctatctcg
     1201 aatacgccat gagcgtggtc aaaggccgcg cgctgcctga agtttcagac ggccaaaagc
     1261 ccgttcagcg gcgcattttg tttgccatgc gcgatatggg tttgacggcg ggggcgaagc
     1321 cggtgaaatc ggcgcgcgtg gtcggcgaga ttttgggtaa ataccacccg cacggcgaca
     1381 gttccgccta tgaggcgatg gtgcggatgg cgcaggattt taccttgcgc tatcccttaa
     1441 tcgacggcat cggcaacttc ggttcgcgcg acggcgacgg tgcggcggcg atgcgttaca
     1501 ccgaagctcg gctgacgccg attgcggaat tgctgttgtc cgaaatcaat cagggaacgg
     1561 tggatttcgt gccgaactac gacggcgcgt ttgacgagcc gctgcacctg cccgcccgcc
     1621 tgcctatggt gttgctcaac ggcgcgtcag gcatcgcggt gggcatggcg accgagattc
     1681 cgtcgcacaa tttgaacgaa gtgacgcagg cagcgattgc gctgttgaag aagccgacgc
     1741 tggaaaccgc cgacctgatg caatatattc ctgcgcccga ttttgccggc ggcggtcaaa
     1801 tcatcacgcc ggcggacgaa ttgcgccgga tttacgaaac cggcaaaggc agcgtgcgcg
     1861 tgcgtgcgcg ttatgaaatc gagaagctgg cgcgcggaca gtggcgcgtc atcgtaaccg
     1921 agctgccgcc gaacgccaat tccgccaaaa tccttgccga aatcgaagaa caaaccaacc
     1981 cgaaaccgaa agcgggtaag aaacagctca accaagacca gctcaacacc aaaaagctga
     2041 tgctggattt aatcgaccgc gtgcgcgacg agtccgacgg cgaacatccc gtgcgactgg
     2101 tgttcgaacc gaaatccagc cgcatcgata ccgatacctt catcaacacg ctgatggcgc
     2161 aaacttcgct ggaaggcaat gtgtccatga acttggtgat gatggggctg gacaaccgcc
     2221 ccgcgcagaa aaacctgaaa acgattttgc aggaatggct ggatttccgc accgtaaccg
     2281 taacacgccg tctgaaattc cgtttgaacc aagtggaaaa acggctgcac atcctcgaag
     2341 gccgtctgaa agtctttctg cacatcgacg aagtgattaa agtcatccgc gaatcagatg
     2401 acccgaaaac cgatttgatg gcggcgttcg ggctgaccga aatccaagcc gaagacattt
     2461 tggaaatccg cctgcgtcag ttggcgcgtt tggaaggttt caaactcgaa aaagaattga
     2521 acgaattgcg cgaagaacaa ggccgtctga acatcctttt gagcgacgaa aacgaaaaac
     2581 gcaagctgat tgtcaaagag atgcaggcgg acatgaaaca gttcggcgac gcgcgccgca
     2641 cgctggtgga agaagccgga cgcgccgtgc tgacacaaac caccgccgac gaacccatca
     2701 cactgattct gtcggaaaaa ggctggatac gcagccgcgc cggacacaat ctcgatttga
     2761 gccaaaccgc gttcaaagaa ggcgaccgcc tcaaacaaac cctcgaaggc agaacgattt
     2821 tacccgtcgt cattctcgat tcatcgggca gaacctacac ccttgatgcc gccgaaatcc
     2881 ccggcgggcg cggcgacggc gtgccggttt catccttgat cgagctgcaa aacggcgcga
     2941 aacccgttgc gatgttgaca ggattgccgg aacaacatta tttattatca agcagcggcg
     3001 gctacggctt catcgccaaa ctgggcgata tggtcgggcg cgtgaaagcg ggcaaagtgg
     3061 tgatgaccgc agacagcggc gaaaccgttt tgccgccggt tgccgtctat gcctcctcgt
     3121 tcatcaaccc cgactgcaaa atcattgccg ccaccaatca aaaccgcgcc ctcgccttcc
     3181 ccatcggcga attgaaaatt atggcgaaag gcaaagggct gcaaatcatc ggattaaacg
     3241 ccggcgaatc gatgacgcat accgccgttt cttccgagcc ggaaatcctg attgaaagcg
     3301 aaggcaggcg cggcgcagcg cacaaagacc gcatccccat ctccctgctt gaggcaaaac
     3361 gcggcaaaaa aggcaaacta ttacccatat cgggcagcct gaaacagctt tcttccccga
     3421 aataaacccg actctgcaca tactatggta atttccaata cccgcgaact tgagaaactc
     3481 aaagaccgga ttcccaatct gatcaacatc gcccgcatcg ccatcgtctt ccccctgctg
     3541 attatgcata tcttcgggtt ggaggtcagc ggacatgaaa acctgcacgc ttcgtggacg
     3601 gcgtgggcat tttatgtttg gctcgccatt tcctgctggc tgattttctt ttccatcatc
     3661 catccgcatt ggcaatggca gtcgctgaaa atgccgcgtt tcagcgcggt ggcggacatc
     3721 acgatgatcg gcgtgctgac ctacctgttc ggcggcatcg attccggctt cggcatcctg
     3781 attctgccct tcgtcgtctg ctcctgcctg ctcagctacg ggcgttaccc cctgctctat
     3841 gccagctacg ccgccatcct gctgatattc aacgccattg ccgacggcga tatcggcaaa
     3901 tatccgcaca tatcggatgc ccgaaccgcc tcggcaacct tcatcctcgt cgccgcctcc
     3961 tatctttccg ccatcttcac ctcactgtcg gtcagataca tcgacagcgc cggcaaactt
     4021 gcccatgaaa accacgccgc ctaccgccgc atcaggggct tgaaccaaat cgtactcaac
     4081 cgcgttcagg aagcggtcgt cgtcatcaac gtcgagcatc agaccatact gttcaataaa
     4141 aaggcaaaag atctgctccc catgcttgaa atcggacagc ataccgccct attcgaccct
     4201 attaccgttt tatgggacaa ggcctcttcg cgtactttcg aacgcaatat cgacacgccc
     4261 gccctgaccg cccgcatccg cgccgtgccg atgaacaaag agcaaaacaa actgctcatc
     4321 ctctacatcc gcccgcaaag cgaaattcag gcagaagccc tgtccgtcaa acttgccgca
     4381 ctcggacaac tgaccgccaa cctcacccac gaaatccgca acccgatgtc cgccatccgc
     4441 cacgccaacg gcctgctgcg cgaaaatatg gaagcggggg cggcagatcc gttcaacgcc
     4501 aaattgtgca aaatcatcga cggcaacgtc tgccgtatca acaaaatgct cgaagatatt
     4561 tcctcgctca acaagcgcaa caaaaccgaa cgcgaagcca tcggcctgat accgttttgg
     4621 gaagaattca aacaagagtt cctgctcggc catcccgatg ccgccggctg catccgccta
     4681 gatatgcagg gcaatcacct gaccgcctat ttcgatcccg cccacctgcg gcaaattatg
     4741 tggaacctcg ccaacaacgc gtggcggcac agccgcaaac agcccggctc gatttccgtc
     4801 accatccgcc ccgcgcaaaa aaacaccgtc tgtatcctct ttgccgaccg cccgaagtgc
     4861 aggaacacct gttcgaaccc ttttacacca cggcggaaaa cggcaccggc ctcgggctgt
     4921 atgtcgcccg cgaactggcg cacgccaatt tcggcgattt gacctaccta ccggaagcca
     4981 aatgtttcga actcacatta ccggaaaaaa ccaatgactg aactgcaaca ccccgtcctc
     5041 gtcgtcgatg acgaaaccga cattctcgac ctgatggaaa tgaccctgat gaaaatgggc
     5101 ttgcgcgtcc ataccgcgtc aggcgttgcc gaagccaaaa acaagctcga cagccaacgc
     5161 tattcgctcg tcctgaccga tatgcgtatg ccggacggct cggggctgga agtcgtccaa
     5221 cacatcaaca gccgcctgct cgatacgccg gttgccgtca tcaccgcctt cggcaacgcc
     5281 gatcaggcac aggaagcgtt gcgttgcggc gcgttcgacc ccgataccat gcagatacag
     5341 gactatctcg accaaatcga acgcgacatt atcgaacaaa ccctcaaaca aaccgaaggc
     5401 aaccgcacgc aggccgccaa acgcttgggc atcagcttcc gttccatgcg ctaccgtatg
     5461 gaacgcctca acatcggctg acgacaaaac ggcatccgca ccatctccgc ccacccgaaa
     5521 aaatgccgtc tgaaacggca cgggaaagcg ggttcgcccc acgcccgaac ggacacaaaa
     5581 caccatgacc gacatcctta ttgacaacac cgccaccgaa accgtccgca ccctgatacg
     5641 ggcattcccc cttgtgcccg tttcccaacc gcccgaacaa ggcagttacc tccttgccga
     5701 acacgatacc gtcagcctca ggcttgtcgg ggaaaaaagc agcgtcatcg tcgattttgc
     5761 ctccggcgcg gcacaatacc ggcgcacaaa aggcgggggc gaactcatcg ccaaagccgt
     5821 caaccacacc gcgcacccca ccgtttggga cgcaaccgca ggattggggc gcgacagctt
     5881 cgtcctcgcc tcgctcgggc tggccgttac cgccttcgag caacatcccg ccgtcgcctg
     5941 cctgctttca gacggcatcc gccgcgccct cctcaatccc gaaacgcaag acaccgccgc
     6001 gcgcatcacc ctgcatttcg gcaacgccgc agtacaaatg cccgcacttg ttcaaacaca
     6061 aggcaaaccc gacatcgtct atctcgaccc catgtatccc gaacgccgca aaagtgccgc
     6121 cgtcaaaaaa gaaatgacct acttccaccg gctcgtcggt gaagcgcaag atgaggccgc
     6181 cctcctccat accgcacgcc aaacggcaaa aaaacgcgtc gtcgtcaaac gcccccgcct
     6241 cggcgaacac cttgccggac aagcccctgc ctaccaatac accggcaaaa gcacccgctt
     6301 cgacgtttac ctgccctacg ggacggacaa gggataacgc ccataaaaca agacaccgaa
     6361 aaatttgccg ttcttatgca aacgagaaac cggtttttgc gtttcgactg ttttggataa
     6421 atcatcacac cttaaagttt gtcattccca cggaagtggg aatccagaaa tgaaaagcaa
     6481 caggaattta tcgtaaatga ccgaaaccga ac
//
LOCUS       DAWHXZ010000112         6479 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000112 DAWHXZ010000000
VERSION     DAWHXZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.112"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            54..197
                     /locus_tag="WIE35_001362"
     CDS             54..197
                     /locus_tag="WIE35_001362"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154272.1"
                     /translation="MMLNQGFCEAFIVVVLMGIAAIMRKRPSFSKPPHRFRVCPSSRP
                     DLQ"
     gene            234..446
                     /locus_tag="WIE35_001363"
     CDS             234..446
                     /locus_tag="WIE35_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ4154273.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            594..2177
                     /locus_tag="WIE35_001364"
     CDS             594..2177
                     /locus_tag="WIE35_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ4154274.1"
                     /translation="MSSKKKKAAFARAATENGVPDVSNIKNSYDDLMYESGAFSQTAI
                     NNLEARARLMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIERMGLENVRLEEKDILTIDESFGKFDYIIVHGIWSWVPDTVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLRDIMQFSSRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDTNLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDILLSGIRDVMKTGEAFKTEDVAENLDRRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTDYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2259..3356
                     /locus_tag="WIE35_001365"
     CDS             2259..3356
                     /locus_tag="WIE35_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ4154275.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANTIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFSWQLSFTDNIGVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQENIPSYAEALKREGHNPFFSA"
     gene            3470..4585
                     /locus_tag="WIE35_001366"
     CDS             3470..4585
                     /locus_tag="WIE35_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ4154276.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     ACGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIIGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4710..5165
                     /locus_tag="WIE35_001367"
     CDS             4710..5165
                     /locus_tag="WIE35_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ4154277.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RSEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5236..5712
                     /locus_tag="WIE35_001368"
     CDS             5236..5712
                     /locus_tag="WIE35_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ4154278.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5700..>6479
                     /locus_tag="WIE35_001369"
     CDS             5700..>6479
                     /locus_tag="WIE35_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ4154279.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMEQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLAASKTD
                     LCTPEQLVEFHDKAAKLFPPKAKVVEVRNAQLDIQWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQV"
BASE COUNT         1684 a         1570 c         1701 g         1524 t
ORIGIN      
        1 aacgacgtac tggtttttgt taatccacta taaatgcgcc gattgccaag gcgatgatgc
       61 tcaaccaggg tttttgcgaa gcgttcattg ttgtcgtcct gatgggaatt gccgccatta
      121 tgcgcaagcg tccgtctttt tccaaaccgc cgcaccgttt ccgcgtttgc ccaagttcac
      181 gccccgactt acaataacgc ccgtcagatt gcagttccaa cgaaaggaaa atgatggaaa
      241 cccttatcct cgacatcggc ggcatgagct gcggcggctg cgtcaaaagc gttacccgga
      301 tattggaagg cgtaaaaggc gtggcaagcg tcgaagtcag ccttgaaaac aaaagtgcga
      361 ccgtcggata cgaccccgcg caaaccgatg caggggcgtt gattgaagcc gttgaagacg
      421 gcggctatga tgccgcgttg aaataaagcg gcaaaaatgc cgtctgaagc cttcgcgcct
      481 cctagatgcc gtctgaagta cgctccgcgc cttcagatgg catttgattt agttgaacgg
      541 gtaggatgaa ttggttaaac ttgcgggcat attgtcttta gagtgaacaa aatatgtcga
      601 gcaagaagaa aaaagccgct tttgcccgtg ctgcaacgga gaacggtgtg ccggacgtgt
      661 ccaacatcaa aaattcatat gatgatttga tgtacgaatc cggcgcattc tctcaaaccg
      721 ccatcaataa tttggaggcg cgcgcccgcc tgatgggctt gcagcccgcg cccgccgcca
      781 atgccaaggt cttggagctg ggctgttcga tgggcggcaa catcatcacg caggcgcttt
      841 attacccgga tgcggaattt gtcggtatcg acttgtccgg caggcaggtt gcgcagggca
      901 acgccatcat tgaaagaatg ggcttggaaa atgtgcgtct ggaagaaaaa gatattttga
      961 ccatcgatga gtcattcggg aagttcgact atatcatcgt ccacggtatt tggtcgtggg
     1021 ttcccgatac ggttaaagac aaaatctttt cgatttgccg gaacaacctg acggaacacg
     1081 gcattgccta tatttcgtac aatgtctacc ccggctggaa acggcaggag cagttgcgcg
     1141 atattatgca gttttccagc agggatgtgc ttgaagaacc cttggaagcg cgaacgcgga
     1201 aaggcttgga cgcgctcaag gcactggcgg aaattttgga aaacgacaag ggcttgggcg
     1261 gcggcggcaa acttccggcg attcaaaaaa tattgaatca taatttttat tacatcgcac
     1321 acgaatatat ggaggcgttt aacgacccga tttacgtcaa cggcttcatc gaatgggcta
     1381 accgccacag gctcgcatat atcggggata ccaatttgca cgtgtctttt gtttcttgga
     1441 tggcggagca tacgcgggag cggattctgg cattggcagg ggacgattat attgccaaag
     1501 aattttacag tgatatttta tccgaccgcc aattccgccg ttcgctttta tgccgtgagg
     1561 aagtcgggga tactgtcagg cgtgatgagt cggttgccgt cgaagtgata gaaagtttga
     1621 atttccgccc ggcaagaggg gaaacaatca actttgatga aaacgatatc ctgctttcgg
     1681 gcatacgcga tgtgatgaaa accggagagg cgtttaaaac ggaagatgtt gcggaaaatc
     1741 tcgaccgccg atttcccggt ctggaatttg accgcatgaa aatcaattcc cagctgttat
     1801 tgcaaaccat tctcgggcgt ttttctgttt catcagacaa tgcgggcaaa ccattttttg
     1861 aagaccataa aacctatgtg ccggcgcgtt ttacagatta tgccgccgcc tttgtggaac
     1921 atggcgcgga agcgtttgtc cggcctgcca accgttacaa cgaaagcacc ccctcattcg
     1981 gatacgggca tttgtacatt atgcggcaat tgtcgcggcc gacgagcaaa caggcattga
     2041 ttgaaacggt tgccgaaaac ttgaacatcg tcagcaccac gcccgacggt ttgacattcc
     2101 atccgcctgc cgaagtgtat gtggaagaaa tattggcaga cttggcagac aggcattttc
     2161 tcgtttcggc ggattgacgg gcaagtcccg gcggcaatcc atccgatata ttttcagaca
     2221 ggaaagacac acactacgaa aagcggtgca cgacacatat gaaccagaca tttaccttgc
     2281 ccgatacgcg cccgtatccc caaaatccga ttaaaaacca cctgctgctc aatgcctacc
     2341 agttggcgca taattcttcc caggcttcgc gcaaactctc gtccggccaa cttcaaaccg
     2401 aaatcagggg gatgcttgag caaaaccact atatcaacct ttccctcgcg ctgacgatgt
     2461 cgcccgatgc cggaacttat gccgcgctgc tttccagtgt gaacgcagta ctcgattgcg
     2521 agaaagaagg cgaagtgcag tggttcgccc tgccggtcgt gctggtgtcc ggctgcaaga
     2581 aagaacgggc aatcgagatg aagctgccga cggaggcatt gtttgcctgc ctgcaaaact
     2641 atccgcacct gcgcgcgttg acgcaagaga cacaatggct gccttatctt gtgcattcgt
     2701 ccgatttgag tgcggtcgcg ccggatgagt ggtggcgtgc caaacaaaat accgaagcgg
     2761 cggcgcaaca cttacgccgt ttcgccccgc gccctttgct gttgcccgaa gggcagtccg
     2821 tccacgtcgt ttacgcgctg ggtttcggca gcggcaaggt tcagacggca ttgggtcaga
     2881 accttcttca ggcaggcctg cccttaatgc aggtatggca ggaaaatctt gcatcggaag
     2941 gtgttacgct gtttgccaat ccgctttccc cggattctcc ggtacgcgcg ctttcagacg
     3001 gcagccacac gcgccagcgt atggcgatgg atgtttttgc ggcaaacacg atacgcgccg
     3061 tccgtatgca gagtccgcgc gtcggcgtgg tcgctgcggc aaaggcgggc ggacaggttt
     3121 tattcgggtt taatgcgacc gacggcgcgt tcgaagtcgt gccgcaggtg ttttcgtggc
     3181 agctttcctt taccgacaat attggcgtta ttcagcaaaa ttttttggat ttaatggcag
     3241 aatgccgtgt agaacacgtt tacctgttgc acaatccttt gggcgaacag gaaaacatcc
     3301 cgagctatgc ggaagcgttg aaacgggaag ggcacaatcc gtttttcagt gcatagtgat
     3361 ttgtagtagt gataggcttt ctcatttcta taataaatca aaataaagag cacaacactt
     3421 tttcattctg tgttgtgcct tgagtgaaac gaaaggatga attataaata tgaagattgt
     3481 aattgcaccg gattcgttta aggaaagctt gacagctcaa caagtagctg aagcaataaa
     3541 aagaggcttc caacaatcga tagcagatgt ggaatgtctc ctctgtcctg tcggtgatgg
     3601 gggagaaggc actgtagatg ctatccgaca ttctcttgac ctagaagaaa aatgtctcca
     3661 agtgacagga ccttttggac aaaaagaagt catgcgctat tttcaaaaag aacaactagc
     3721 cctattcgaa gttgctgatt tggttggtct tggaaaaatc ccgctagaga aacgaaatcc
     3781 attacaaatc caaactcgtg gtattggaga attgattcgc cacctcatta gtcaagaaat
     3841 taaagaaatc tatattggcg ttggcggtac ggccagtaat gatggaggta ttgggattgc
     3901 tgctggttta ggttatcaat tttatgatga ggatggaaat accttgcccg cttgcggtca
     3961 atccttacta aacctagctt ctgtttcaac agaaaatcgc tatgaaattc ctgaagatgt
     4021 tcacattcgt attttagcag atgttgtgag tcccttatgt ggacaccaag gtgcgaccta
     4081 tacgtttggc aagcaaaaag ggttggattc tactatgttt gaggccgtag atcaggcaat
     4141 acaagatttt tatgaaaaag tctcccctgc aacattaaaa cttaaaggag caggagctgg
     4201 tggaggcatc gctggtggtt tgtgcgcctt tgctcaggca agtatcgtat ctggaattga
     4261 cacctgcctg gacttgattg actttgataa gaaagtgtca gatgttgact tggttatcat
     4321 tggtgaagga agactagatc gtcaaagttt agcagggaaa gcgcctattg gtgtagcaaa
     4381 aagaacccct gtcggagttc ctgttgtcgc tatttgtggc agtctcgttg aagatttgcc
     4441 ttccctgcca tttgaaaata tccaagctgc cttttctatt ttggagaaaa gtgaaccttt
     4501 agaagatagt ttgaaaaatg ccagtctcta tttggagcat acggcttcta atattggaca
     4561 cttattaaat atgcctaaga tttagccaaa ccatttcttc cagatggatg ttttggctgg
     4621 ttctgcctta tgcggttacg attaaccgac cgatgacgtc tgaaagggtt tccgcttcag
     4681 acggcatcgc gccggaacag ggaggggata tgaaaaaacc gaaaatcctt tttgtctgcc
     4741 tcggcaacat ctgccgttcg ccgatggcgg aatacatttt gcgccgccgc gccgccgaag
     4801 cgggcattcc ccttgaagcg gacagcgcgg ggacatcggg ctggcacgac ggcgaagata
     4861 tgcaccgcga gacggcaaag atattgaaaa aacacggtat cgatgcttca ggctttacca
     4921 gccgcaaaat ccgccaaagc gatgcggcgg cgtttgactg catcatcgcg atggacggca
     4981 agaatttgtc cgaattggaa agaaccttcg gcaggcggtc ggaaaaaata ttcaagctga
     5041 ccgacctgat acccgaaagc ggttacgacc atgtccccga tccgtggtac acgggcgatt
     5101 ttgaagaaac cttcaggctt gcggatgcgg gctgtcgggc attgttggaa aagatttcca
     5161 aataaagcaa ttgaatacaa atataaaacc gcctcctgcc gtatcggttg ttcagacggc
     5221 ataaacagag aatttatgaa aacaaatttc aaacagaaaa ttattgaaca ggcacgcagc
     5281 gaaggcttgc aggtaaccgc tttgcgcgag caggtattag atattgtctt gcagcaaagc
     5341 ggcgtgatta aagcctacaa cgtcttgtcg cagatgcagc agcaaagcga gggtgtgctt
     5401 gcaccgccta ccgcctaccg cgcccttgat ttttgggcgg agcagggcgt tttgcacaaa
     5461 gtggcggcgg tcaacggcta tattttgtgc agccacgcgc agcacgagtg cgacgaccat
     5521 tgccacgacc acgaagaagc cgaagcgcac cacagcgcgt ttattttggt ctgcaccgaa
     5581 tgtggcacgg cggacgagca aaccctcagc cacgagtggg cggcactgcg cgcaggcgtt
     5641 gccgaaagcg gctttgcgct gaaagaagaa cacgttgttt taaccgggat ttgtaaaaaa
     5701 tgtcagaaat gaaaaaaacc aaagtccacc tgatttcagg ttttctggga acaggcaaaa
     5761 ccaccgcgct caaaagtctg atggaacaaa aagaccccaa cgaaaaatgg gtcatcatcg
     5821 tcaacgagtt tggcgaaatc ggcattgacg gcgcggtatt gagcgacaac ggcatccctg
     5881 tggcagaaat cgccggcggc tgtttgtgtt gcaccgccgg cccgcaaatg ggcgtaaccg
     5941 tgcagaaaat gctgcgcgat gccaagcccg accgcctgat gattgaggca agcggactgg
     6001 cgcacgccgc cagcgtcatc gacgaactca aagccaaacc gctggacagc cttttggaaa
     6061 tcggcgcggt ctttaccgtc gtcgatccgc gccaattcat caaccccgat tacgcgcaac
     6121 aggcattgta taaagaccaa atcggcattt gcgacgtatt ggccgccagc aaaaccgatt
     6181 tatgcacccc cgaacagctg gtcgaatttc acgacaaagc ggcaaaactg ttcccgccca
     6241 aagccaaagt ggtcgaagtc cgaaacgcgc aactcgatat ccaatggctc gatattcccg
     6301 tcatcgaaaa atcacgctac cgcctcaaag ccctgccgga caacacgatg ggcttccagt
     6361 cgcaaggttt cacattcccc gccggacgcg atttcgacgg cgaaaaattg accaacttct
     6421 tcaatgattt gcccaagatg accgaaggac tcgtccgcgc caaaggcgtg tttcaagta
//
LOCUS       DAWHXZ010000113         6461 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000113 DAWHXZ010000000
VERSION     DAWHXZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.113"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            150..1691
                     /locus_tag="WIE35_001370"
     CDS             150..1691
                     /locus_tag="WIE35_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ4154280.1"
                     /translation="MDGRRWVVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFLDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFALGIFLLLDNGEGGK
                     QTETL"
     gene            1720..2319
                     /locus_tag="WIE35_001371"
     CDS             1720..2319
                     /locus_tag="WIE35_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ4154281.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESQIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2316..2909
                     /gene="nadD"
                     /locus_tag="WIE35_001372"
     CDS             2316..2909
                     /gene="nadD"
                     /locus_tag="WIE35_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ4154282.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRAGVSDGIPPAAARYIREHGLYEK"
     gene            2968..3354
                     /gene="rsfS"
                     /locus_tag="WIE35_001373"
     CDS             2968..3354
                     /gene="rsfS"
                     /locus_tag="WIE35_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ4154283.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            3408..3878
                     /gene="rlmH"
                     /locus_tag="WIE35_001374"
     CDS             3408..3878
                     /gene="rlmH"
                     /locus_tag="WIE35_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ4154284.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAVPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVFLTEQLYRAVSILHNHPYHRE"
     gene            3951..4217
                     /locus_tag="WIE35_001375"
     CDS             3951..4217
                     /locus_tag="WIE35_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ4154285.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4374..5057
                     /locus_tag="WIE35_001376"
     CDS             4374..5057
                     /locus_tag="WIE35_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ4154286.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPASAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            5181..5693
                     /gene="coaD"
                     /locus_tag="WIE35_001377"
     CDS             5181..5693
                     /gene="coaD"
                     /locus_tag="WIE35_001377"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ4154287.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            5725..6378
                     /locus_tag="WIE35_001378"
     CDS             5725..6378
                     /locus_tag="WIE35_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ4154288.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRCGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1402 a         1701 c         1858 g         1500 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactatat ttcagacggc gggaggaagg
       61 cggatacggt atgtagtcgg attgaaatat acggtatgtg ggggcttttg ggtacaatcg
      121 caacatcagt gtttcaagtg gggaaggcga tggatggacg gcgttgggtg gtatggggtg
      181 cttttgccct gctgccttcg gcttttttgg cggtaatggt cgttgcgcct ttgtgggcgg
      241 tggcggcgta tgacggtttg gcgtggcgcg cggtgctgtc ggatgcctat atgctcaaac
      301 gtttggcgtg gacggtattt caggcagcgg caacctgtgt gctggtgctg cctttgggcg
      361 tgcctgtcgc gtgggtgctg gcgcggctgg cgtttccggg gcgggctttg gtgctgcgcc
      421 tgctgatgct gccttttgtg atgcccacgt tggtggcggg cgtgggcgtg ctggccctgt
      481 tcggggcgga cgggctgttg tggcgcggca ggcaggatac gccgtatctg ttgttgtacg
      541 gcaatgtgtt tttcaacctt cctgtgttgg tcagggcggc gtatcagggg tttgtgcaag
      601 tgcctgcggc acggcttcag acggcacgga cgttgggcgc gggggcgtgg cggcggtttt
      661 tggacattga aatgcccgtt ttgcgcccgt ggcttgccgg cggcgtgtgc cttgtcttcc
      721 tgtattgttt ttcggggttc gggctggcat tgctgctggg cggcagccgt tatgccacgg
      781 tcgaagtgga aatttaccag ttggtcatgt tcgaactcga tatggcggtt gcttcggtgc
      841 tggtgtggct ggtgttgggg gtaacggcgg cggcagggtt gctgtatgcg tggttcggca
      901 ggcgcgcggt ttcggataag gcggtttccc ctgtgatgcc gtcgccgccg cagtcggtcg
      961 gggaatatgt gctgctggcg tttgcggcgg cggtgttgtc tgtgtgctgc ctgtttcctt
     1021 tgttggcaat tgttgtgaaa gcgtggtcgg ccggcgaatc gtggcgtgtg ttaatggaaa
     1081 gtgaaacgtg gcaggcggtg tggaatactt tgcgcttctc ggcggcggcg gtgtatgcgg
     1141 cggcggtttt gggtgtggtg tatgcggcgg cggcgcggcg gtcggcgtgg atgcgcgggc
     1201 tgatgttttt gccgtttatg gtgtcgccgg tttgtgtttc ggcgggcgtg ctgctgcttt
     1261 atccgcagtg gacggcttcg ttgccgctgc tgctggcgat gtatgcgctg ctggcgtatc
     1321 cgtttgtggc aaaagatgtt ttatcagcct gggatgcact gccgccggat tacggcaggg
     1381 cggcggcggg tttgggtgca aacggctttc agacggcatg ccgcatcacg ttccccctct
     1441 tgaaaccggc gttgcggcgc ggtctgactt tggcggcggc aacctgcgtg ggcgaatttg
     1501 cggcaacctt gttcctgtcg cgtcccgagt ggcagacgct gacgactttg atttatgcct
     1561 atttggggcg tgcgggtgag gataattatg cgcgggcaat ggtgttgaca ttgctgttgt
     1621 cggcgttcgc gctgggcatt ttcctgctgt tggacaacgg cgaaggcgga aaacagacgg
     1681 aaacgttata atgtgaaccc tttttcagag gacggcaaaa tgagcgagtt ggataacatc
     1741 cttgcccata accggcagtt tgtcgagtcg ggcgaatatg aaaaatactt taccgacaaa
     1801 taccccgaac gcgggctggc agttttgtcc tgtatggatg cgcggattat cgggctgctg
     1861 cccgacgcgt tgggtttgaa aaacggcgat gccaagctga ttaaaaatgc cggcgcgctg
     1921 gttacgcacc cgtggggttc ggtgatgcgg agtcttttgg ttgccgtgtt tgaattgaag
     1981 gtcagagaga ttatggtcat cgcccatcac gattgcggta tgcaggggct gaatgccgaa
     2041 gaattcctcg ggcgcgtccg ggaaagccag attcccgaag accgtatcga aaccctgcgt
     2101 tatgccggta tcgacctcga cggctggctg accggtttcg acaacgtcga agacagcgtg
     2161 cgccacacgg tggaccttat ccgtaaccat ccgctgatgc cgcgccatat cgccgttcac
     2221 ggactggtca tccatcccgt taccggcaaa ctgacgctgg ttgtggacgg cagtgtttca
     2281 gacggcatgg acttatcgga aggaatggaa acatcatgaa gaaaatcgga ctgttcggcg
     2341 gcactttcga cccgatacac aacgggcatc tccacatcgc ccgcgccttt gccgacgaaa
     2401 tcggcttgga cgcggttgtt ttcctgccgg caggcggccc gtatcacaaa gacgcagcct
     2461 ccgcttccgc cgccgaccgc cttgccatgg tcgaactggc gacggcagaa gacgcgcgtt
     2521 ttgccgtcag cgattgcgac atcgtccgag aaggtgcaac ctatactttt gataccgtcc
     2581 aaatcttccg ccagcagttc ccatccgcgc aactctggtg gctgatgggc agcgacagcc
     2641 tgatgaagct gcacacatgg aaaaaatggc agatgctcgt gcgcgaaacc aatatcgccg
     2701 tcgccatgcg gcagggcgac agcctgcacc aaaccccgcg cgaactgcac gcgtggctgg
     2761 gcaagtccct tcaggacggc agcgtccgca tcttgtccgc cccgatgcat aatgtgtcgt
     2821 caacggaaat ccgccgcgcc ggtgtttcag acggcatccc acctgccgcc gcacgctaca
     2881 tccgcgaaca cggtttgtat gaaaaataaa gtcaaatcag taagaaacgg ctataatgcc
     2941 gtctgaaaac atcccgttag gaaaataatg aacgaacaag aactgcaaga cctgcaaaaa
     3001 atggtcgggg tcgccgtcaa cgccctcgaa gacatcaaag ccaaagacat ttccgttctc
     3061 gaaacgcaag acaaaacttc gctgtttgcc agaatgatta tcgccagcgg cgacagtacg
     3121 cgccaagtca aagcactggc caacaacgtt gccgtcgatt tgaaagaagc cggttttgaa
     3181 atcctcagta ccgaaggaga cagcggcgaa tggacgctcg ttgatgcagg agacctcgtc
     3241 gtccacgtta tgctccccgc cgtgcgcgac ttctatgata tcgacaccct ttggggcggc
     3301 gagaaaccga gttttcacgc cggaatgcag aagccgtggc acgcggcaga ctgattcccg
     3361 atgccgtctg aatgttcaga cggcattttc ctgtaaggga gaaagcattg aacattaccg
     3421 ttttggcagt cggcaccaaa atgccgcgct gggttgatga ggccgtcgcc gaatacgcca
     3481 aacgcttcgg acgcgacgtc gcctacgcac tcaaagaaat caaacccgaa aaacgcggcg
     3541 cgggcgtgaa tgccgcccaa ggtatggcgg cggaagaaaa acgcatcctc gaagccgttc
     3601 cgcaaggcgc gttcctcgtc gttcttgacg aacgcggcaa agcgccgacc tccgtcgagc
     3661 tggcggaaca cctcaaaagc tggcggcaaa acggcgaaca cgtctgcttc gtcatcggcg
     3721 gcgcggacgg catgaccgac cgcctcaaac aacaagcccg catgatgatg cgcctgtcca
     3781 gcctcaccct gccgcacggc atggtgcgcg tctttctgac cgagcagctc taccgcgccg
     3841 tttccatcct gcacaaccat ccctatcatc gggaataaga aggcttttgt ccgtatccct
     3901 aatcggttaa aatcaccccg ttatttctga acacagcaaa ggaatccgct atggcacgca
     3961 tggtattctg cgtcaagctc aacaaagaag ccgaaggcat gaaatttccg ccgcttccca
     4021 acgaattggg caagcgcatt tttgaaaacg tatcgcaaga agcgtgggcg gcgtggacgc
     4081 gccaccaaac gatgctgatt aacgaaaacc gtttaagcct cgccgatccg cgcgcgcgcg
     4141 aatacctggc tcagcagatg gagcagtatt tcttcggcga cggcgcggat gccgttcagg
     4201 gatacgttcc gcaataacgg ttttccgttt tgaacacagg ctgtccgaaa ctgcttcaga
     4261 cggcctttaa aatacgccgg acaactttat cttcacgcaa ccggacaaat tttgacattg
     4321 ggcaatgtta aaatccccca ttattcccaa ccgcgctttc aggagcagat gatatgcaac
     4381 acgacgttta cgactacacc gcgcatacgg tttctaaaaa caccgtcctg cagaaaacct
     4441 accgcctgct cggattttca tttattccgg cttccgcagg cgcggcactt gccgccaatg
     4501 ccggtttcaa tttttacgcc gccttcggtt cgcgctggat agggtttgcc gtcgtgttgg
     4561 cgtttttcta cggtatgatc cacttcatcg agaaaaaccg ttacagcaat accggcgtta
     4621 ccctgctgat ggtattcaca ttcggtatgg gcgtattgat cggccccgtg ctgcaatacg
     4681 cgctccatat tgccgacggt gcgaaaatcg tcggcattgc cgccgcgatg accgccgccg
     4741 tctttttaac gatgtccgcc ttggcgcgcc gaacccggct cgatatgaac gcgctcggac
     4801 gcttcctgac cgtaggtgcg gtcattctga tggtcgccgt ggttgccaat ctgtttttgg
     4861 gtattcccgc actcgccctg accatttccg ccggttttgt cttgttcagt tccttaatga
     4921 ttatgtggca ggtacgcacc gtcatcgacg gcggcgaaga cagccacatc agcgcggcac
     4981 tgacactgtt tatctcgctt tacaacatct tcagcagcct gctgaacatc ctgttgtctt
     5041 taaacggcga ggattgatgc cggtaaaacg ccgtccgact atgccgtctg aaactgcttc
     5101 agacggcatt ttactttggg catacaatac ggatgcacac gcaccaacac acagccgact
     5161 atgaagccgg gaggaatcaa atgttaccga atacaccgcg ccgcgccgtt tatgccggca
     5221 gtttcgatcc gcccacattg gggcatctgt ggatgatacg gcaggcgcaa tctatgtttg
     5281 acgaactcat cgtcgccatc ggcattaacc ccgacaaacg cagcacctat accgtcgctg
     5341 aaaggcagga tatgttgtgc gatattacta aaatgtttcc caacgtcaga accgatgtat
     5401 ttgaaaaccg atttttggtg cattacgccc gtgaggtaga tgcaggattc atcgtgcgcg
     5461 gcatccgttc tgcttcggat tacgaatacg aacgttccat gcgccatatc aacagcgacc
     5521 tcgcccccga aatatccacc gtattcctca tgccgccgcg cgaaatcgcc gaagtgtcgt
     5581 ccactatggt caaaggactg gtcgggccgg aaggctggac ggaaacgatc caccgctacg
     5641 tgccgcaagc tgtgtacgaa aaaatccttg ccgaacatca acacgaaaat tgaacccttc
     5701 tatttttgca aggcatatat ggatatgttg gaaattttgt tccgccatca agacttcgtc
     5761 gccatcaaca aaccgggcgg catatccgtc caccaagaca gcggcgagac cggactggcc
     5821 cgaacactcg ccatacagtt gggcgtagag cgtgtgtggc tgctgcaccg attggacaaa
     5881 cagaccagcg gcattttact gtttgccctc aacaaagaaa gcgcgtccgc ccttagcgga
     5941 cagtttgccg gcaaaagcat caaaaaaacc tatttggcac tgtccgaccg caagccgtcc
     6001 aaaaagcagg gttggattaa aggcggaatg gaaaaatcca gatgtggaat gtggaagttg
     6061 acgcgcaata cagaaaatat cgccgttacc cgattccaca gcatcagtat cgccgaaaaa
     6121 ctgcggctgt tcatcctaga accgcatacg ggcaaaacgc accaattgag agtggcgatg
     6181 aaaagtttgg gcagtccgat tctgggcgac agcttatacg gcggaacaga atccgaaacc
     6241 atgtttctgt atgcatggaa aatacaattt gcctaccaaa accgacaaat cgaaattgtt
     6301 gcacccttaa aaaacgaatg gcagactgaa aatatctctc atgcgctgga agaattttat
     6361 atggcaaaag caggttaata aatataaatt aatttataaa caatatgata aatattttta
     6421 taaaaatttt tcaagaaaac ggttgactgc aaatctcttc a
//
LOCUS       DAWHXZ010000114         6383 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000114 DAWHXZ010000000
VERSION     DAWHXZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.114"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            176..1234
                     /gene="alr"
                     /locus_tag="WIE35_001379"
     CDS             176..1234
                     /gene="alr"
                     /locus_tag="WIE35_001379"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ4154289.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1298..2653)
                     /locus_tag="WIE35_001380"
     CDS             complement(1298..2653)
                     /locus_tag="WIE35_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ4154290.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVKRGGRIPAPVQSMK
                     N"
     gene            complement(2668..2940)
                     /locus_tag="WIE35_001381"
     CDS             complement(2668..2940)
                     /locus_tag="WIE35_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ4154291.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3066..3803)
                     /locus_tag="WIE35_001382"
     CDS             complement(3066..3803)
                     /locus_tag="WIE35_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ4154292.1"
                     /translation="MKKLSRIVFSIVLLGFSASLSAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAYAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGSKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4084..4983)
                     /gene="prmB"
                     /locus_tag="WIE35_001383"
     CDS             complement(4084..4983)
                     /gene="prmB"
                     /locus_tag="WIE35_001383"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ4154293.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLTVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5128..5721
                     /locus_tag="WIE35_001384"
     CDS             5128..5721
                     /locus_tag="WIE35_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154294.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            5833..>6383
                     /locus_tag="WIE35_001385"
     CDS             5833..>6383
                     /locus_tag="WIE35_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ4154295.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIATAAMKA
                     AIPDYMKFGEGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVAKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVDRPANPDAVFTCFKADKGAEAVNNDTEHNAT
                     YFSHFPSKQKSTGGSRCPFLYRFP"
BASE COUNT         1742 a         1604 c         1467 g         1570 t
ORIGIN      
        1 atttgggaaa gcgattatat caggaaaagc aaaccgcctt cctacctgaa aactgctgct
       61 tcggcttgaa gacacaaggg tctttaatat tttaaaagcc ttgccgttgg attataattc
      121 cctaattgat ttcttaattt tgctaataaa cacttgcttg gtaaggaatg aatttatgcg
      181 ccctttgaac gtgcagatca ggttgggcaa ccttaggcac aattatcgga ttttaaagga
      241 aatgcacgga ggcaagctgt tggcggtagt gaaggccgac gcatacggac acggtgcggt
      301 cagatgtgct ttcgcgctgg cagacttggc agacggcttt gccgtggcga caatcgatga
      361 aggaatcagg ctgcgggaga gcggcattac ccatccgatt gtccttttgg aaggcgtatt
      421 tgaagcatca gagtacgaag cggtcgaaca atactcgctc tggccggcag tcggaaacca
      481 atggcaactt gaggctttgc tttcccgcca ttggaaaaaa cctgtcaagg tctggttgaa
      541 aatggattcg ggaatgcacc gtaccggatt tttccctcat gattacgctt cggcatatgc
      601 ggcattgaaa cagtcggaat atgtggacag tattgtcaaa ttctcgcatt tctcctgtgc
      661 ggacgaaccc gaaagcggta tgacggaaat acagatggaa gcattcgatt tgggtacgga
      721 agggctggaa ggcgaagaaa gccttgccaa ttcggcggct attttgaatg ttcccgaagc
      781 acgcagggat tgggggcgcg cgggcttggc gttatacggc atttccccgt tcggaggcag
      841 cgatgacagg ctgaaacccg tgatgaggct ttcaacccgt atttttggcg aacgcgtttt
      901 acagccgcac tcccctatcg gttatggcgc aacattttat accagcaaat ctacgcgcgt
      961 cggtctgatt gcctgcggtt atgcggacgg ttatccgcgc cgcgctccaa gcaattcccc
     1021 cgtcgccgtc gacggtaaat tgacccgggt catcggcagg gtctctatgg atatgatgac
     1081 catcgagctg gacgcttcgc aagaaggttt gggacacgag gtcgaactgt ggggcgatac
     1141 ggtcaacatc aatactgttg ccgaagcggc cggaaccatc ccttacgaat tgatgtgcaa
     1201 tatcaaacgt gcaaaattca cttatatcga gtaatcgagt ccaaacaaaa atgccgtctg
     1261 aagcctttca gacggcattt ccccatcaaa accgcaatca gtttttcatt gattgaaccg
     1321 gagccgggat tctgccgccc cgtttgacga acacttcgca cgaaccttct ttgaccggca
     1381 ttacaggcgc gtagcctaac aggccgccga actcgacact gtcgccgacg gttttaccgg
     1441 ttaccggaat aatgcgcacg gcagtggttt tgctgttgat catgccgatg gcggcttcgt
     1501 cggcaatgat gccggaaatg gtgtgcgcgg gtgtgtcgcc gggaacggca atcatatcca
     1561 agccgaccga acaaacggcg gtcatcgctt cgagtttatc caacgtcagc acgcctgctt
     1621 cggcggcggc aatcatacct tcgtcttcgg aaacggggat aaacgcgcca ctcaaaccgc
     1681 caaccgcgct cgaagccatc atgccgccct ttttcacggc atcgttcagc aatgccaaag
     1741 ctgctgttgt gccgtgcgta ccgcagacgc tcaaacccat ttcttcaaga atgcgcgcca
     1801 ccgagtcgcc gacggcaggg gtcggtgcca gcgacaagtc gagaatacca aacgggatat
     1861 tcagcatttt tgaggcttcg cggccgatga gttcgcccac gcgggtaatt ttgaaggcgg
     1921 ttttcttcac aacttcggca acttcggtca atgtcgttgc atccgaattt tccaacgcgg
     1981 cttttacgac acctgggccg gatacgccga cattaatcac agcatccgct tcgcccgatc
     2041 cgtgaaacgc acccgccata aacggattgt cttccaccgc attgcagaac acgacgattt
     2101 tcgcgcagcc gaaaccttcg ggcgtaattt cagccgtgcg tttgatggtt tcgcctgcca
     2161 gcttgaccgc atccatattg ataccggcgc gcgtactgcc gacattgatg gagctgcaca
     2221 cgatgtcggt ggtcttcatc gcttcgggaa tggaacggat taacacctcg tctgaaggcg
     2281 acatgccttt ttgcaccaac gcggaaaagc cgccgataaa ggacacgccg atggctttgg
     2341 cagccttatc caaagtttgc gccacgctga cataggaatc ggcatgggta gccgccgcga
     2401 tttgggcaat cggcgtaacg gaaatgcgct gattcacaat cggcacgccg tatttggcag
     2461 acagatattt tgccgtcgcc accaagtctt tgccgaccgt ggtaattttg ttgtaaatgt
     2521 tttggttcaa cacattgata tcgctgctga tgcagtcgtg cagatcaatg ccgatggtaa
     2581 tggtgcggac atcaaaattc tggtcggcaa ccattttgac ggtttctaaa atttcgccgg
     2641 attggatact catcacattc ctccgactca aatgcggtgc atcgcttgga agatttcttc
     2701 attttgcata cggatatcaa gcgcgagttt tttgctctct tccgcaaaca aatccaaaac
     2761 ctcctgccga gatttggggc atttcgaggt atccaccaag ataatcatgg taaaaaaatc
     2821 atccatcagc tgttggctga tattgaggat gttgattcgg ttttccgcca aaattttgga
     2881 aacatcgtac acgatgccga cgcggtcttt accgatgacg gtgatgactg aattgttcac
     2941 aggcttactc cttgcagata tccgttaaag tccgaaatta taccaccgtt ggattttgaa
     3001 gaaatattgt caacaatata tacatacaaa atgccgtctg aaactatttc agacagcatc
     3061 aagattcagg gttcgattaa ataaccatcc ttatcccact gggttttcct gaccaacttg
     3121 tcatcctgat aaacagcttc actcttttta gaaccatctt cataccactc taagactact
     3181 ccattacgtt tatgatgatg gatagacagt tccgagagta atcgaccgct ttcatcccat
     3241 gtcagaatct tggccggctc atcattgacc ataaccattt ccgttttgat attgccatca
     3301 gcataccatt gtttccatac gccgtttgcc ttattttgtt taaactggat ttcgctttcc
     3361 ttgctgccat tacggtaata gcggtacccc gtaccttcac tcaaaccatt tttataaggc
     3421 ataaccgcag attttttacc gttcggatac cagttgaccc actcaccgtc cggcttaccc
     3481 ttgctgaagc cccccgccat ttttttctga ccattaaaat gccacaaaat caacataccg
     3541 ttttgcaggg taggcacaaa agatttgatt tgcgttgaag caacgatata aggttcggaa
     3601 tatttcttca tcgacggata ataaaaatcc tgcgcgtacg caataccctc cgccacacta
     3661 tattgcctga tataagcggc agaagacatc gtcgccgtca gtttcccgtt ctgattaaaa
     3721 taaacagaat aggtctgtgc cgataaagaa gccgaaaaac ccaacaggac aattgaaaat
     3781 acaatccgag ataatttttt cattgcaata gcgatataaa aacaaggctg tgttttagta
     3841 atctgttgat ttcaattatt tgcaagggaa aagacaatta ttttccggtt aggaataaac
     3901 ctattctact gaatatattg aagccaagta cgcctatcaa cactatatta aaacactgcc
     3961 aaaaacaatt aacttataaa caatatggta aggatttctc tgccaagcat caaacccgag
     4021 acaacgtatc gtaaaaatgc cgtctgaaaa caaatcgtct tcagacggca tttccccttc
     4081 aactcactct tcacccaata actgctcgcg cgtcaagagg aaaacaaaac cgtcgccccc
     4141 gctggtttcc aaccaagtaa aaggcaactc cggatacgct gcttccaata catccctgtt
     4201 atgcccgatt tccaccagca atacaccttt gggattcaga aactttgccg cattcagaag
     4261 aatctgcctg gtggcatcca acccgtccgc cccgctgccc aatgccaatt ccggttcgtg
     4321 caaatactct tcaggcaata actcaaccga ttccgcatcc acataaggcg gattggaaac
     4381 aatcaaatca taagtgcctt ccaatccttc aaacaaatcc gtatgaataa gccggatgcg
     4441 ttcttccaaa ccataatctt cgacattaat ccctgccact tccaaagcat ccaagctcac
     4501 atcaaccgca tcaatttggg catcaggata atgatgcgcc atctgaatgg caaggcaacc
     4561 gcttccggtg caaagatcca aagcattatg caccaactca tcgtattcta tccaaggacg
     4621 aagtccgtca cccaacaatt cataaataaa agaacgaggt atgattacgc gctcatccac
     4681 atagaaatca aactctccct gccatgcctg gtgtgtcaaa taagcggctg gaatgtgttc
     4741 gacagcacga cgctcaataa ccgtcagcac ttcctctttt tcagcttcca agagttttgc
     4801 atcaagatat ggggcaagca tatccaaagg caaattcaaa gtatgcagaa tcaaataagc
     4861 tgcttcatca tgcgcattat ccgttccatg accaaaaaag agccctgcct cattaaaacg
     4921 gctgactgca aaacgtaaaa catcgcggat agtcgtcaat tcttgtgctg cctgattaaa
     4981 cataatatga accattctgc gtatagatac ttttaattat aacagaaaca acaagcaaac
     5041 cttttcatat cgccaaataa ccacccaatc tacccataca actacataaa tgcccgcgcg
     5101 aaaaccatcg cccgaacgga aacgacaatg gccgacggta tgggcaacct gattggctgg
     5161 gaaaaaacgg ggcttgttgc cggtaagcag tggataaccg caaaagacga caaggtgtcc
     5221 gatgtctgca atgccaacgg cgagatgggc gtaatcgggc tttacgagcc tttctcacac
     5281 ggcgcattga cgatacccgg tcatccgaac tgccgatgcg aggttgtttc cgtatcgggt
     5341 ggcgaattgg gggaatttgc cgaaaaaaag gagcttcgta aagcggctat gcagtatgcg
     5401 cgggataact ttatcggcaa aagctatgtc aatgaaaaca gcgggcatga actgaaggta
     5461 acttggcaag gtgtgaaaca cgctgcgtca aaggcaaatc aggcggaatt atccatcatg
     5521 acaaaacttg atgacttatt gcgctacgca aaatatgagg gttcttattc ggataggaaa
     5581 ggtcatccta atattattgc agcacataag tatcgtgccg ttgccaaggt tgggaatgag
     5641 tctttaaata tcggtgtgat tgtaagggaa tttccagacg accataaaca ttacgaccat
     5701 ttcatcttga aggatgaata aagccctttt gcagtgtcgt tctggagcgg atagcgttaa
     5761 ggcaagtaca cttccagcct tgaaaaaggg ctttaaattc agcatgccat ttatacaggc
     5821 aggagtaaac ccatgacaaa gttatacgca gaaatcgcca agatggagac gcaggacgac
     5881 gacacggtca aggtttgggg ttacgcttca agcgaggaaa tcgattcgga cggcgaagtc
     5941 atcgcgacgg cagctatgaa ggcggcgatt cccgattata tgaagtttgg cgaggggcgc
     6001 gagatgcacg gctcaaacgc tgcgggaacg gcaattgaaa tcaacgttga agacgacggc
     6061 ataacctttt tcggtgctca tatcatcgac cccgttgtcg tggcgaaagt caaaacaggc
     6121 gtttacaaag gcttttccat cggcggcagc gttaccgccc gcgatgattt gaacaagtcc
     6181 caaatcacgg gcttgaagct gacggaaatc agccttgtcg accgccctgc caatcccgat
     6241 gcggtgttta cctgctttaa ggcggacaaa ggtgcggaag cggtaaacaa cgatacagaa
     6301 cataatgcta catattttag ccatttccct tccaaacaaa aaagcaccgg cggcagccga
     6361 tgccccttcc tttacaggtt ccc
//
LOCUS       DAWHXZ010000115         6333 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000115 DAWHXZ010000000
VERSION     DAWHXZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.115"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(77..1531)
                     /locus_tag="WIE35_001386"
     CDS             complement(77..1531)
                     /locus_tag="WIE35_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ4154296.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACEII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(1528..2229)
                     /locus_tag="WIE35_001387"
     CDS             complement(1528..2229)
                     /locus_tag="WIE35_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ4154297.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPTDLEENA"
     gene            complement(2226..3005)
                     /locus_tag="WIE35_001388"
     CDS             complement(2226..3005)
                     /locus_tag="WIE35_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ4154298.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPNMPRPKHIASFLLERTGGKA"
     gene            3289..4695
                     /locus_tag="WIE35_001389"
     CDS             3289..4695
                     /locus_tag="WIE35_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ4154299.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIATDL
                     DESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFILGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRMYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSSLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTLS"
     gene            4807..6333
                     /locus_tag="WIE35_001390"
     CDS             4807..6333
                     /locus_tag="WIE35_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ4154300.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
BASE COUNT         1369 a         1834 c         1577 g         1553 t
ORIGIN      
        1 tttttgttaa tccactatac aaatgccgtc tgaagccctg tttcgggttt cagacggcat
       61 ttttgcgaac aactttttat gcctgttctt tttggcgcat tttttctgcc attagttcgt
      121 gcaaggtttt cttcgccggt ttcatcggca cgcggttttg cgtccaaccc aactgtttgc
      181 gtggggtcag gttgcggaac ttggtggctg cccaaccgaa ggcgcggtag gttttgctgc
      241 cgctgaaaat accgttgaat gtgcgccacg ccatttgttc gccgaaggta tgcgatgcgc
      301 cttgccctcg gatggggtgc ggcacggttt cggtcggcga acgttgcgct tcaacacgca
      361 gacgttgcat ttgttcggta atcggaatgc gtaccggaca aacttcaacg cacgcgccac
      421 acatagtaca cgcagtcggc agatcgcgtg tcgcatccaa gcccaacaaa tgcggagaga
      481 taatctcgcc aatcgggccg ggataagtcg taccgtaagc cgcaccaccg atacgggtat
      541 aaaccggaca gtggttcata cacgcaccgc aacggataca ttgcaaggtg cggcgcattt
      601 gatcttctgc gtaagcttgg ctgcggccgt tgtcgagcag aaccaagtgc atttcttgcg
      661 gaccgtctaa ttcttcactg cggcgcgggc cggtaatcat attgaaatag gttgtgatgt
      721 tttgaccgat ggcggagcgc ggcagcagac tgtacaaggg tgggatgtcg gacaatttcg
      781 ccaccacttt ttcaatgccg gtaatagcga tatgcacggg cggtacggtg gtactcaagc
      841 gaccgttgcc ctcgttttcc accagacaca gcgtacctgt ttcagcaacg gcaaagttta
      901 cgccactcaa accgacatcg gcagtgctgt aaatatcgcg cagtgcttta cgggcgaagc
      961 cggtcagttg gtctacatcg tctgtcagcg gcgtaccgag gttttggtgg aacagttcgc
     1021 taacctgttc tttggttttg tggatagcag gcatcacgat atgggtcggt ttttcacctg
     1081 ccatttggac gatgaactcg cccaagtcgc tttccaccgc tttaatgcct tttgcttcaa
     1141 gataatggtt cagctcgatt tcctcgctga ccatcgattt gcctttgacc atcagcttgc
     1201 cgtttttggc tgtgatgatg tcgtggatga tttcgcaggc ttcggccggt gtttccgccc
     1261 agtgtacttt tacgcccaac ttggtcaggt tttcttccag ctgctccagc agggcaggca
     1321 atttagacaa tgagcgctga cggacgtgtt cgcacaaatc gcgcaagctt tgcagctctt
     1381 cttcgtcggt caaaacagct ttgcgtttgg tcatcagcat atccatcgcg gtacgcaggc
     1441 ttttacgcaa aggcttgtct tgaagggaaa ttgcggcgtt ttgcttgaaa gtttccggct
     1501 tcatgtgaaa tttgatggtt tgcgtagtca tgcgttttcc tccaaatcgg taggggaaat
     1561 gtggtcgggc aggatggcga ggataaccaa atcacgcgga ccgtgcgcgc cgtaagcaag
     1621 cgtcagttgg atgtctgcgg ttttggacgg accggaaatc aggaatacat tggtcggcat
     1681 accgttttcc accagttttt cgccttcgac ggcattatga aactcgttgt acatcttgga
     1741 cgtatcgaac aggcagaaat gcacgggcgg aacgagactc aaagtacgcg gctcttcggg
     1801 gctggaaaac agcatcagcg tgccggtgcg ggcgatgccg cattgcgcgc cgctgaagcc
     1861 cgcatcgatg ttcgtgaaaa actcggtttt ccaagtatcg atttcgcgct cgaaggcaat
     1921 cggttcgata ttgctgtccg ccaatgcggc acgggcaatt tgtccgtgtt cggtcgccaa
     1981 gggcagcagg atgtttttca aacccttgcc ttctgccgct tcgcggaaaa cctgcatcca
     2041 attgcttttc gtcacccaat aaatttcggt tttgaccgcg cgcattgcgg cagcccaatg
     2101 tttcagacgc tcggcttcgc tgccccaaga aacacccatt tcacggtaat aatcaaaaac
     2161 cgcaggttct tccatcggca atgcgtcggc ttttttcagt tttgccaaaa tattttcgcg
     2221 cgcgctcatg ctttgcctcc ggtacgttcc aacaagaagg atgcgatatg tttcggacgc
     2281 ggcatattcg gttcgtcctt agcaattttg ccgccgatgt tcatcataca gccgcagtcc
     2341 gcgctgatga tttcggttgc gccggtttct ttcagcgcgg cgactttgtc ggttaccatc
     2401 gcgccggaaa tatcggcttg tttgacggag aatgtgccgc cgaagccgca acattcgctt
     2461 tcgtggtcgt ggacgatgcg ttcgacgttt tccataccgt caatcagttg ccagcccgag
     2521 agatggacat tcatttcgcg gcgggcggcg caggaagtgt gaacggcgac tttgagcggt
     2581 tcgcccttgt cttcgggttt gaaaccgatg gcaagcagga aatgggtaaa ctcgatgatg
     2641 cggccggcgc aatccacagc cctttcctcg tactcgctgc ctttaaacag cgtcggccaa
     2701 tggtgcttca tcataccgcc gcacgaaccg gacggtacga caatcggcca ctcctcgggg
     2761 aaaaggtcga gttgcgctct ggcgacatca aaggcttcgg tcggatggcc ggatgaatag
     2821 gcgggctgac cgcagcagct ttgcgccatc gggaaatgga cgcgtatgcc ctgttgctcg
     2881 attagggtaa tggcatccat gcctgcttcg ggcataaaaa gatcaaggac gcaagtgccg
     2941 aaaaaataga catcggtcgg attgctgtcg tatcggatga tttttggggg aatggttgcg
     3001 ctcatgtttt ttgataacgg gaaacccgtt tttttctgta gaaaggtaag cgtttacttt
     3061 aagtaattga ctgttgcggg tcaagtctaa ttttaaaaaa taatccggtt tttcttacaa
     3121 actgccccat aacgcttact gtaccttact ctgataattt tcgataataa ttatcattac
     3181 aatgaaatgc cgattcgttt gcttgtgaac attcaggacg ccgactctga cggcattcag
     3241 acagcatctg aaaacaataa cggcacaagg atggggtaca atcgccctat ggaaaaaaac
     3301 accctatccg cccgcgcacc atccccttgg ctgccgcttc tgctggcaat tgccattttt
     3361 atgcagatgt tggatgcgac cattttaaat accgcactgc ctgaaattgc caccgacctg
     3421 gacgaatcgc ctctggatat gcaactggca gtcgttgcct atacgctgac ggttgccctg
     3481 ctgattcctt tgagcggtta tttggcggac aggttcggaa cgaaaaaagt ctttttcggt
     3541 tcgattgccg tttttatact cggatcggca ttgtgcgccg catcgggttc gctgtttgaa
     3601 ttgacgcttt cccgtgtcgt tcagggcatc ggcggttcga tgctggttcc gataccgcgt
     3661 ctgaccatct tgcgtatgta cgacaagtcc aagctgctca atgccatcaa ttatgcggtc
     3721 atgcctgcat taatcgggcc ggttttaggg cctttggcgg gcggttattt ggtcgaatac
     3781 gcttcgtggc actggatttt cctgctcaac ctgcccatcg gtctgctggg tttcatattg
     3841 ggacgcaaca tcatgcccga tattaaaggc agcaatatct ctttagactt caaaggctat
     3901 ctgatttttt ctgccgccgc gtgcctcttg ttactttcgg cagaaagcct gtcgcacgcg
     3961 ctgcctccgt attttgcact gttgccgctg tgcggcggac tgctgtttgc acgccgttat
     4021 ttccgacata tgaaaaccgc gtccaaaccg atttattccg ccgacctgtt tctgatacgc
     4081 actttccgtc taggactggc gggcaatctg ttcagccgtc tcggcatcag ctcgattcct
     4141 tttctgatgc ccctgatgtt tcaagtcgct ttcggcttcg gcgcaagcct gtcgggttgg
     4201 ctggtcgccc ccgtcgccct gtcttcgctg ctggtcaaac cgctgattgc accgctcatg
     4261 aaacgtttcg gctaccgcac ggtactgctt tggaacacca agctgcttgc cgccttcatc
     4321 atgctgctcg ccctgcctga cggaaactcg tcgctgtgga tttggatttt cctctcgctg
     4381 gcgatcggcg cgtgcaactc cctgcagttt tctgccatga atacactgac cctcgccgat
     4441 ttgcgcccgc aacagaccgg cagcggcaac agcctgatgg cggtcaacca acagcttgcc
     4501 atcagcatgg gcattgttgt cggcgcatta atccttaaaa actggacatt tctgataccg
     4561 gcttcttcag gtctgcattc cgccttccgt atgaccctgc tcagcatcgg cggcatcacc
     4621 cttgcctcgt cgttggtttt caagcgtctg cacgtttcag acggcgcaaa cctgacacgg
     4681 aacacactgt cctaaagcgg tccgcacgca aaacttttac ccgtttcaac gtttggatta
     4741 tgattccgca catccatgcg cgccaacccc aaaacacagg caatgccgtc tgaaaccata
     4801 tccctgatga aaacacgcag cctaatttcc cttttatgcc tccttctctg ttcatgttct
     4861 tcatggttgc ccccactgga agaacggacg gaaagccgtc atttcaatac ttccaaaccc
     4921 gtccgcctgg acaacatcct gcaaatccgg cacacccctc ataccaacgg gctatccgat
     4981 atctatctgc tgaacgaccc ccacgaagcc tttgccgccc gcgccgccct tatcgaatct
     5041 gccgaacaca gcctcgattt gcaatactac atctggcgca acgacatttc cggccgactg
     5101 ctgttcaacc tcgtgtacct tgccgcagaa cgcggtgtgc gcgtacgcct gctgttggac
     5161 gacaacaaca cgcgcggatt ggacgacctc ctgctcgccc tcgacagcca tcccaatatc
     5221 gaagtgcgcc tgttcaaccc cttcgtctta cgaaaatggc gcgcactcgg ctacctgacc
     5281 gacttccccc gcctcaaccg ccgcatgcac aacaaatcct ttaccgccga caaccgcgcc
     5341 accatactcg gcggacgcaa tatcggcgac gaatacttca aagtcggtga ggacaccgtt
     5401 ttcgccgacc tggacatcct cgccaccggc agcgtcgtcg gcgaagtatc gcacgacttc
     5461 gaccgctact gggcaagcca ttccgcccac aacgccacgc gcatcatccg cagcggcaac
     5521 atcggcaagg gtcttcaagc actcggatac aacgacgaaa cgtccagaca cgcgctcctg
     5581 cgctaccgcg aaaccgtcga acagtcgccc ctctaccaaa aaatacaggc aggacgcatc
     5641 gactggcaga gcgtccaaac ccgcctcatc agcgacgacc ctgcaaaagg actcgaccgc
     5701 gaccgccgca aaccgccgat tgccgggcgg ctgcaagacg cgctcaaaca gcccgaaaaa
     5761 agcgtctatc tggtttcacc ctatttcgtc cccacaaaat ccggcacaga cgcactagca
     5821 aaactggtgc aggacggcat agacgttact gtcctgacca actcgctaca ggcgaccgac
     5881 gttgccgccg tccattccgg ctatgtcaaa taccgaaaac cgctgctcaa agccggcatc
     5941 aaactctacg agctgcaacc caaccatgcc gtccctgcca caaaagacaa aggcctgacc
     6001 ggcagctccg taaccagcct gcacgccaaa accttcattg tggacggcaa acgcatcttc
     6061 atcggttcgt tcaacctcga cccccgttcc gcgcgtctca acaccgaaat gggcgttgtt
     6121 atcgaaagcc ccaaaatcgc agaacagatg gagcgcaccc ttgccgatac cacccccgaa
     6181 tacgcctacc gcgttaccct cgacaggcac aaccgcctgc aatggcacga tcccgccacc
     6241 cgaaaaacct acccgaacga acccgaagcc aaactttgga aacgcatcgc cgcaaaaatc
     6301 ctatccctgc tgcccataga aagtttatta tag
//
LOCUS       DAWHXZ010000116         6311 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000116 DAWHXZ010000000
VERSION     DAWHXZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.116"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..274
                     /locus_tag="WIE35_001391"
     CDS             <1..274
                     /locus_tag="WIE35_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ4154301.1"
                     /translation="DLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVH
                     TLNGSGLAVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(352..1350)
                     /locus_tag="WIE35_001392"
     CDS             complement(352..1350)
                     /locus_tag="WIE35_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ4154302.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAHDPFCNPEAEKIGGRYVDLDELYAQSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQIGDCGNAVRADG"
     gene            complement(1475..2551)
                     /gene="prfA"
                     /locus_tag="WIE35_001393"
     CDS             complement(1475..2551)
                     /gene="prfA"
                     /locus_tag="WIE35_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ4154303.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(2655..3425)
                     /locus_tag="WIE35_001394"
     CDS             complement(2655..3425)
                     /locus_tag="WIE35_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ4154304.1"
                     /translation="MNFTDTHCHLADPALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDFVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANIPGIAKIAAEIRGTCVEEIAKVTERNADALLGR"
     gene            complement(3433..4425)
                     /locus_tag="WIE35_001395"
     CDS             complement(3433..4425)
                     /locus_tag="WIE35_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ4154305.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWSYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TASGFDNPHFGVIAEWDETQGWYNLRIPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSEGWGAEDVHKELVRLKLV"
     gene            complement(4492..5175)
                     /locus_tag="WIE35_001396"
     CDS             complement(4492..5175)
                     /locus_tag="WIE35_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ4154306.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(5364..>6311)
                     /gene="glmM"
                     /locus_tag="WIE35_001397"
     CDS             complement(5364..>6311)
                     /gene="glmM"
                     /locus_tag="WIE35_001397"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ4154307.1"
                     /translation="EIEAKIDEEMKTQPSARLGRARRISGADDRYIEFCKSTFPSHSD
                     LRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEKCGATYTKTLQAAV
                     LQHEANYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREGINIGGVVGTVMTN
                     MAMENALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILCMDKHNTGDGIISA
                     LQVLAALQTLNQDLATVCADWQPYPQTMINVRIGKGQNWQEASKDVLAEVEKELEGKG
                     RVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
BASE COUNT         1362 a         1735 c         1721 g         1493 t
ORIGIN      
        1 tgatttggaa gtttgggtgc cggcgcaaaa tacctaccgc gaaatctcaa gctgctccaa
       61 ctgcgaagat ttccaagccc gccgcatgaa ggcgcgtttc aaagacgaaa acggcaaaaa
      121 ccgcttggta catactttga acggctccgg cttggctgtt ggcagaacgc tggtcgccgt
      181 attggaaaac catcaaaacg ccgacggcag catcaacatc cctgccgcac tgcaaccgta
      241 tatgggcggc gttgccaagt tggaagtgaa ataagtttgc aagctgcctg aacgtcaaat
      301 gccgtctgaa acctggttca gacagcattt cctttaaact tttaaaacac gtcagccgtc
      361 ggcacgaacc gcattgccgc aatcgccgat ctgtccgacc tcgcggatat tggacagcgt
      421 aacttccgaa atattaccca acgcctcttc cgtcaaaaac gcctgatgac cggtaaacag
      481 cacgttatgg caggacgaca accggcggaa cacgtcgtcg gtaatcacat cgttggattt
      541 gtcttcaaaa aacagctcgc gctcgttctc gtacacatcc atgcccaatg cgccgatttt
      601 ccggcgtttc aacgcctcaa tcgcggcggc actgtcaatc agcccgcccc ggctggtgtt
      661 gataatcatc acgccgtctt tcattttgtc gaacgccgct tcgttcagca tatagtggtt
      721 ttccggcgtg gcggggcaat gcagcgtgat gatgtccgac tgggcataca gctcgtccaa
      781 atccacatac ctgccgccga ttttttccgc ttcggggttg caaaacggat cgtgagccag
      841 caggttcatg ccgaaaccct ttaaaatccg catggttgca ataccgattt tccccgtgcc
      901 gataacgccc gccgttttgc cgtacatatt gaaaccggtc agaccttcca gcgaaaaatt
      961 cgcatcgcgg gtacgctgat aggctttgtg gatacggcgg ttcaaagtca gcatcagacc
     1021 gaccgtatgt tccgcaaccg attcgggcga ataggcaggc acgcgcacga ctttcaagcc
     1081 caactcttca gccgccttta aatccacatt attgaagccg gcacaacgca acgccacagt
     1141 tttcacgcca atttgcgcca atttttccaa cacgggccgg ctgccgtcgt cgtttacaaa
     1201 aatacaaacc gcttccgcgc cttccgccat tttcgccgtt ttcgcatcca gcataaaatc
     1261 aaaaaactcc agctcgaagc cgaaatgccg gttggcgcgg gtaaaatgtt cgcggtcata
     1321 gcttttcgta ccgtaaatcg caatcttcat caatatgtcc agttgtcgtc tatggttgag
     1381 aaacggcata atacactgaa ttcaaacaaa tcagtagaat atggtggatt aaaattgatt
     1441 gcatgcacgg catttccatt tcaaaacaca aaactcaatc gcccattgcc gccaaaagct
     1501 ccgcctgatg ttcggcaatc agggcatcgg tgatttctgc caaatcgccg tccatcacaa
     1561 aatccagctt gtgcagggtg aggttgatgc ggtggtctgt taccctgcct tggggatagt
     1621 tgtaggtgcg gatgcgttcg ctgcggtcgc cgctgccgat gagggatttg cgttcggcgg
     1681 cttctttggc ttgggcttcg cgtttttgcg cgtcgttcag gcgggcggcg agaactttca
     1741 ttgcctgcgc tttgttggcg tgttggctgc ggccgtcttg gcattcgacc accatgccgg
     1801 tgggcaggtg ggtaatgcgg acggcggaat cggttttgtt gatgtgctga ccgcctgcgc
     1861 cggatgcgcg gaaggtgtcg atgcgaaggt cgacggggtt caactcgatg tcttccagtt
     1921 cgtccgcttc gggcatgacg gcaacggtgc aggcggaggt gtggatgcgg ccttggcttt
     1981 cggtggcggg gacgcgctgc acgcggtgtc cgcccgattc aaatttcaaa cggctgtacg
     2041 cgcctaagcc gataatacgg gcgataactt ctttatagcc gcccaactcg ctttcgttgg
     2101 cggacacgat ttcaacctgc cagcggttgc gttcggcgta gcggctgtac atacgcagca
     2161 aatcgccagc aaacagcgcg gcttcgtcgc cgcccgttcc ggcgcgtact tcgataaaga
     2221 tgtttttgtc gtcgtcggca tctttgggca gcagcagttt ttgcaattcg gtatcgagtg
     2281 cgccgatttt ggctttcgcc gcttcgattt cttcggcggc aaagtctttc atttcggggt
     2341 cggacaacat ttcttcggca tccgccaaat cgctttgcgc cagttgatag ttttggaata
     2401 cttcgacgac gggggtcagt tcagcgtgtt cgcgcgtgag cttgcggtag ttgtccatat
     2461 cggatgtggc ttcgggttgc cctaaaaggt gggtaacttc ttccaaccgg tcgccgagtt
     2521 gttgcagttt ttctaagata gacggcttca taattcttcc ataacaaacg ccgcctgaat
     2581 ttcagacggc atcaacactg aattattata ataggttttc cggacattca aaaagataat
     2641 ctcagatgga taacctaccg tcccaacagg gcatcggcat tgcgctccgt taccttggca
     2701 atctcttcca cacaggttcc gcggatttcc gcagcaatct ttgcaatacc cggaatattg
     2761 gcaggcgtat taatctcttt tttcagcata aacgggctgt cggtttccaa tacgaaatcc
     2821 ccgtcgttca aggctttaag cgtatcgcgc actttacgcg cgttcggatt gagcaacaac
     2881 gaaccgatgc cgattttgaa acccagtttc gtcaacacgc gcgcttcttc cgcgctgcct
     2941 gagaaggcgt gaacgatgcc gccttgggca aagcctgtct gtttgacggc ggcggcgatg
     3001 tcggcggtgg ctttgagatt atggataatc acgcggcggc gcagggtttg cgcgatggca
     3061 agctggcgga cgaaaacttg gatttgccgt tcgcgctgcg gcggcgtttg ggttttgtcg
     3121 taaaaatcca aaccgatttc gccgacccat gcctgcggat aacgtgccaa catcgcttcc
     3181 aggcggacga aatcacgctc ggcaatgccg tctgaaaacc aaggatggat gccgagcgca
     3241 atacggattt gaccgtgttc ggacggcatt tccgacagtt ccgccacgct ttgccaatcc
     3301 tgcggacagg tggcgggaac gataaaccgc ttcaccccaa ctttccgcgc ggcggtcaaa
     3361 atgtgcggca ggttttcgcg taaggcggga tcggcgaggt ggcagtgggt gtcggtgaag
     3421 ttcattccgg tttcacacta actttagtct tactaattct ttgtgaacat cttccgcgcc
     3481 ccatccttcg gaaacggcaa gcgtcatcaa tgcggttgct gtttccaaat tgcatttgcc
     3541 gccgttgatg atgcctgagt tgcggaacgc gttgccttgc gcgtaaacgg cagcggttct
     3601 gccttgtcgg acttggctga tgttgagcag cagtttgcct tgctgcgtaa agtcttgcac
     3661 ggcgcggatg aaaccttcgt ctgcgggcgt gttgccgtgt ccgtaacttt gcaggataag
     3721 ggcgtgggcg ggaagctgtc cgagtccgtc ggcaagttct tggacggcaa agccgggaat
     3781 aagcgtgcgg acggcgattt ttgcctgcgg gtcaggatag cggattttga ggccgtctga
     3841 aacggccgcc gcatcttggg acggaatacg aagattgtac cagccttgag tttcatccca
     3901 ttcggcgatg acaccgaaat gcggattatc gaagcctgag gcggtttcgg tgctgacttt
     3961 gctgctgccg acggcgggat aaagtttgcc gtcaaaggca ataagcgtct gcttcaatcc
     4021 gagtgggaat gcggcaacgg cagtcgccag attgcgcggc gcgtcgctgc ccacggcgtt
     4081 gtacgaccat tgggaaccgg tcaggacgat cggcttgtcc aaaccctgca gcgcgagggc
     4141 gaacagattg gcggtatagg cgagcgtgtc cgtaccgtgc agcaccaaaa cgccgtcgta
     4201 ctgcggcagc gcggcggcaa tgatgtccag ccaagcgcac cagttttgca gttggacggc
     4261 ggaagaatca atcagcgtcc cacaaacgtg ccaatcgaaa tccatgccgt ttgaaaacgg
     4321 cgcgagtgcc tgcccgacca gcgcggtatc ggggcgcagg ccttcgctgc tttgcgtcat
     4381 gccgatggtg ccgccggtat aaaggacaaa aattttctgt ttcatggctc atcctgaaaa
     4441 tgcaaagacc gcctgaaacg cgaaatgcgt ccggacggtc gaaaatctga tttactgctg
     4501 cttctgtgcc gcttttttcg ctgccttttc cgccttgcgt ttggcgcgtt tttcgctcaa
     4561 ttgtgcgcgg taaagctgat aatgtcggcg tttgcgccac cagaaccacg ccagcgccgc
     4621 cgccagcacg cccaatgcga tgaagatgcc ggattgcagg ctgtgcattt tcgccatcag
     4681 ccaatcgatg ttgtgcgcgc cgtactcgcc caagtaaatc caaacgggca cggaaatcag
     4741 cgcggcaagc ccgtccataa tcagaaagcg cagatacgat actttgcggc tgatgccggc
     4801 ggtaacgaaa acggcagtcc gcaaacccgg caggaagcgg gcgacaaaca gaacccagtt
     4861 gccgtatttg tcgaattttt cctgaacctg tgcgtaacgt ttcggcgtca tgatgcgcgc
     4921 aatcggtttg aacttgagga ttttctgccc ccaaattcgt ccggcggcga acatgatgcc
     4981 gtccccgacc aatacgccga gcataccgac tgcaaacata atatgcggat tggtataacc
     5041 catacccgaa atcacgccgc ctgttaccaa ggtcaaatcc tcggggatcg gcacgccgaa
     5101 accgcagatg accaaaacga aaaacacggc cgcatagccg tattcgacaa aaaaggcttc
     5161 caaaagggca aacatagcgg atattccatt gtcggagata aaaagtcaga acaagccgaa
     5221 acattttctg cgcgaaacgg cattctatca aagattatgc cgtctgaaag cggcaagggg
     5281 cagattcgcg ccctctgccg gacgttcaga cggcatttaa acaggaaaac cgccgctgtt
     5341 gaaacggttt tgcctgattt tgcctaaagg ctgccgatgg cggaagcaat acgttccgca
     5401 ccgtcgcgcg cccaatccgc ctgccgcgcc tccaccatca cacgcacgac gggttcggtt
     5461 cccgaagcgc gcaacacgac acgccctttg ccttcgagtt ctttttccac ttccgccaac
     5521 acgtcttttg aggcttcctg ccagttttgc cctttgccga tacggacatt aatcatcgtt
     5581 tgcggatacg gctgccaatc ggcgcaaacg gtggcgaggt cttggttcag cgtttgcagt
     5641 gccgccaaaa cttgcagcgc ggaaataatg ccgtcgccgg tgttgtgttt gtccatgcac
     5701 aaaatatggc cgctggcttc gccgccgatg agccagccgc gttggttcaa ctgttccaac
     5761 acatagcggt cgccaacttt ggcgcggcag aaatcgacgc cctgctcttt cagggcgttt
     5821 tccatcgcca tattggtcat gaccgtgccg accacgccgc cgatgttgat accttctcgg
     5881 gcgcgggctt tagcgatgac gtaaatcagg ctgtcgccgt cgtaaacctt accgttttta
     5941 tcgaccatca tcaagcggtc gccgtcgccg tctaaggcga tgccgtagtt ggcttcatgc
     6001 tgtaaaacgg cggcctggag tgtcttggta taagtcgcac cgcatttttc attgatattg
     6061 tagccgttgg gttcgtcgcc gatgctgacg acttgtgcgc ccagttcgtg aaacaccttg
     6121 ggagcgacac cgtaccccgc gccgttggcg gtatcgataa ccaacttcaa accccgaagg
     6181 tcggaatggc tgggaaaggt ggatttgcaa aattcgatat agcggtcgtc cgcaccgctg
     6241 atgcggcggg cgcgcccgag gcgtgcagaa ggctgggttt tcatttcttc gtcgattttg
     6301 gcttcgattt c
//
LOCUS       DAWHXZ010000117         6290 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000117 DAWHXZ010000000
VERSION     DAWHXZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.117"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..140
                     /locus_tag="WIE35_001398"
     CDS             <1..140
                     /locus_tag="WIE35_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ4154308.1"
                     /translation="SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLSDA
                     V"
     gene            224..943
                     /locus_tag="WIE35_001399"
     CDS             224..943
                     /locus_tag="WIE35_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ4154309.1"
                     /translation="MATLSDKTILITGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASTESKGRSGEIVYL"
     gene            1108..1779
                     /locus_tag="WIE35_001400"
     CDS             1108..1779
                     /locus_tag="WIE35_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ4154310.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            1789..2157
                     /locus_tag="WIE35_001401"
     CDS             1789..2157
                     /locus_tag="WIE35_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ4154311.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            2154..2801
                     /locus_tag="WIE35_001402"
     CDS             2154..2801
                     /locus_tag="WIE35_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ4154312.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            3244..4386
                     /locus_tag="WIE35_001403"
     CDS             3244..4386
                     /locus_tag="WIE35_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEZ4154313.1"
                     /translation="MQKWQTELYSTPLWLCLTPKSTVKVLLLITVMVTLLLTEVRLNV
                     LSTFMSKGLYDSMQDLNASAFWMFAAMNAGVVLIRAFNNVVNDFLDQGLAIKWSEQLN
                     EVLTTRWLADKNYYRLQMRRHAPDNIDQRIQQDAQDFIASTIEFVRGMVNSVVTSLEF
                     AVVLWGLAGILTVFGFDIPRGIVWLVFIFVILATFIAMWIGNPLIRYNYENEKLNGDY
                     RYSLIRVRDHAESVAFYNGEQHEHGRLSDRFKAIIRNRWRIARQSVCLSGFNDMFTNG
                     IKLFPIILQAPRLFAGQIKIGDIQQTVQAFARLQNALSFFRMFYNKFTAYRARLERLY
                     GFLLSTEEQHSARQPEISEVSDGIALECKAAAAFARIGGSFCTAEA"
     gene            complement(4496..4705)
                     /locus_tag="WIE35_001404"
     CDS             complement(4496..4705)
                     /locus_tag="WIE35_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154314.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPTYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(4702..4998)
                     /locus_tag="WIE35_001405"
     CDS             complement(4702..4998)
                     /locus_tag="WIE35_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ4154315.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            complement(4977..5582)
                     /locus_tag="WIE35_001406"
                     /pseudo
     CDS             complement(4977..5582)
                     /locus_tag="WIE35_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5662..5949
                     /locus_tag="WIE35_001407"
     CDS             5662..5949
                     /locus_tag="WIE35_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ4154316.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVCFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            5921..6223
                     /locus_tag="WIE35_001408"
     CDS             5921..6223
                     /locus_tag="WIE35_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ4154317.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
BASE COUNT         1488 a         1757 c         1619 g         1426 t
ORIGIN      
        1 tgtcgtggtt gcaccggcac cgcatcttcc ccgccggcga ctacctccac ccagactacc
       61 ccgccacgct cgaagccgcc gtacaatcag gtttcgcgtc ggcggaagcc tgcctgcaaa
      121 gcctgagcga tgccgtctga aaacgccggc cggtatcggg acgctttaca aggtgcggca
      181 aaacgctaaa atacccccga ccgaccacca aaaaggaaaa agtatggcga cactgtccga
      241 caaaaccatc ttaattaccg gcgcatcgca aggtctgggc gaacaggtcg ccaaagccta
      301 tgcggcggca ggcgcaaccg tgattctggt ggcacgccat cagaaaaaac tggaaaaagt
      361 ctatgacgcg attgtcgaag ccggacaccc cgaacccttc gccatccgtt tcgacctgat
      421 gagtgcggaa gaaaaagaat tcaatcagtt cgccgccacc attgccgaag ccacgcaagg
      481 caaactggac ggcatcgtcc actgcgccgg ctatttttac gccctctcgc cgctggattt
      541 ccaaaccgtc gccgaatggg tcaaccaata ccgcatcaac accgtcgcac ctatggggct
      601 gacccgcgcc ctgttcccgc tgctgaagca gtcgcccgac gcgtccgtca tcttcgtcgg
      661 cgaaagccac ggcgaaacac ccaaagccta ctggggcggc ttcggcgcgt ccaaagccgc
      721 gttgaactac ctgtgcaaag tcgccgccga cgaatgggaa cgcttcggca acctgcgcgc
      781 caacgtcctc gtccccggcc ccatcaattc cccgcaacgc atcaaatccc atccgggcga
      841 agccaaaagc gaacgcaaaa gctacgggga cgtgctgccc gcatttgtct ggtgggcaag
      901 taccgaaagc aaagggcgga gcggcgaaat cgtttacctc taaatccgga aaaggcaggc
      961 atcccgcccg cgccgcgatt ccgtttgaaa aatatccgta ccttccgcgc gcatccgtcc
     1021 cgcacagaca aacaccttga cttaaattta cataaggaca ataatgataa cccgttattt
     1081 ccttaacttg accttagggt aaattttatg aaaaccgttt ccaccgccgt tgtccttgcc
     1141 gccgctgccg tttcactgac cggctgtgcg accgaatcct cacgcagtct cgaggtagag
     1201 aaagtcgcct cctacaatac gcaatatcac ggtgttcgta ccccgatttc cgtcggaaca
     1261 ttcgacaacc gctccagctt ccaaaaaggc attttctccg acggggaaga ccgtttgggc
     1321 agccaggcaa aaaccattct ggtaacgcac ctgcaacaga ccaaccgctt caacgtactg
     1381 aaccgcacca atttgaacgc attaaaacag gaatccggca tttccggcaa agcgcataac
     1441 ctgaaaggcg cagattatgt cgttaccggc gatgtaaccg aattcggacg cagagatgtc
     1501 ggcgatcatc agctcttcgg cattttgggt cgcggcaaat cgcaaatcgc ctatgcaaaa
     1561 gtggctctga atatcgtcaa cgtcaatact tccgaaatcg tctattccgc acagggcgcg
     1621 ggcgaatacg cactttccaa ccgtgaaatc atcggtttcg gcggcacttc cggctacgat
     1681 gcgactttga acggcaaagt tttagacttg gcaatccgcg aagccgtcaa cagcctggtt
     1741 caggctgttg acaacggcgc atggcaaccc aaccgttaag gagaaatgat gaatccgaaa
     1801 accttgagcc gtttgtcgct gtgtgcggcg gtcttggctc tgaccgcctg cggcggcaac
     1861 gggcaaaaat ccctgtatta ttacggcggc tatcccgata ccgtctatga aggtttgaaa
     1921 aacgacgaca cttcgttggg caagcagacc gaaaagatgg aaaaatactt tgtggaagcc
     1981 ggcaacaaaa aaatgaatgc cgccccgggt gcgcacgccc atctgggact gctgctttcc
     2041 cgttcgggag acaaagaggg cgcgttccgc cagtttgaag aagagaaaag gctgtttccc
     2101 gaatcgggcg tatttatgga cttcctgatg aaaacaggta aaggaggcaa gcgatgaaac
     2161 ctttgatttt agggcttgcc gccgtgttgg cgctgtctgc ctgccaagtt caaaaagcgc
     2221 ccgatttcga ctacacgtca ttcaaggaaa gcaaaccggc ttcaattttg gtggttccgc
     2281 cgctgaacga atcgcccgat gtcaacggaa catggggtgt actggcttcg accgccgcgc
     2341 cgctttccga agccggctat tacgtcttcc ccgccgcagt cgtggaggaa accttcaaac
     2401 aaaacggctt gaccaatgcc gccgatattc acgccgtccg gccggaaaaa ctgcatcaga
     2461 ttttcggcaa tgatgcggtt ttgtacatta cggttaccga atacggcact tcatatcaaa
     2521 ttttagacag cgtgacgacc gtatccgcca aagcacggct ggtcgattcc cgcaacggaa
     2581 aagagttgtg gtcgggttcg gccagcatcc gcgaaggcag caacaacagc aacagcggcc
     2641 tgttgggggc tttggtcagc gcagtggtca atcagattgc caacagcctg accgaccgcg
     2701 gttatcaggt ttccaaaacc gccgcataca acctgctgtc gccctattct cacaacggca
     2761 tcttgaaagg tccgagattc gtcgaagagc agcccaaata agcatatgcc gtctgaacct
     2821 ctccgagctt cagacggcat atgcgaatct tgatacttgc aagccgcacc gcccaaaatc
     2881 gggcggccgg tttcaatatc cgaacaaggt tcgggttctg tattttgcag aacccgaacc
     2941 ttttattcaa atttaaactt aattccggcg tggttgtatg gtatagtgga ttaacaaaaa
     3001 tcaggacaag gcgacgaagc cgcagacagt acaaatagta cggaaccgac tcacttggtg
     3061 cttcagcacc ttagagaatc gttctctttg agctaaggcg aggcaacgct gtactggttt
     3121 aaatttaatc cactataaat taacacgcaa tgtaccctga ccggcaaaac ttccccaaac
     3181 ttcatcaaaa aatcaactgt acccgtcttt ttcagacggc attgataccg taagaaattt
     3241 cccatgcaga aatggcaaac cgagctttat tccacaccgc tttggctttg cctgacaccg
     3301 aaaagcacgg tcaaagtctt gctgctgatt acggtaatgg ttacgctgct gttgaccgaa
     3361 gtacggctga atgtgttgag tacctttatg tccaaaggac tttacgactc gatgcaggat
     3421 ttgaacgcct ccgcgttttg gatgtttgcc gcgatgaacg cgggcgtggt gctgatacgg
     3481 gcgtttaaca atgtcgtcaa cgacttcctc gatcaaggct tggcgattaa atggtcggag
     3541 cagctcaatg aagtgctgac gacgcgctgg cttgccgaca aaaactacta ccgcctgcaa
     3601 atgcgccgcc acgcgccgga caacatcgac caacgtatcc aacaggacgc gcaggatttc
     3661 atcgcttcga ccatcgaatt tgtacgcggc atggtcaatt cggtcgttac ctctttggaa
     3721 tttgccgttg ttttgtgggg cttggctggc atcctgaccg tgttcggctt cgacattccg
     3781 cgcggcattg tttggctcgt ctttattttt gtgattttgg cgacctttat cgcgatgtgg
     3841 ataggcaacc ccttgattcg ttacaactat gaaaacgaaa aactcaacgg cgactaccgt
     3901 tattccctca tccgcgtacg cgaccacgcc gaaagcgtag cgttttacaa cggcgaacaa
     3961 cacgaacacg gccggctttc cgaccgcttt aaagccatta tccgcaaccg ttggcgcatc
     4021 gcgcggcaaa gcgtctgctt aagcggcttt aacgatatgt tcaccaacgg catcaaactc
     4081 ttccccatta ttttgcaagc gccgcgcctg tttgccggac aaatcaaaat cggcgacatc
     4141 cagcagaccg tccaagcctt tgcgcgactg caaaacgcgc tatctttttt ccggatgttc
     4201 tacaacaaat tcaccgccta ccgcgcccga ttggagcgtc tgtacggctt tttgctgagt
     4261 accgaagaac aacacagcgc gcggcaaccc gagattagcg aagtttcaga cggcattgca
     4321 ctggaatgta aggcggcggc agcttttgca cgaattggcg gcagcttttg caccgccgaa
     4381 gcgtaggaaa ttgtattagc ttttgcacaa atttatcccc gtttaatggt ctgttaaacg
     4441 gggattatct tatgccttta aaagtagtac gctacttgcg gtcagaactt ataaatcagg
     4501 tggtcgtcgt agcttgaatt gctgttgatg tgctgctcac agtcaaggtt tggctgcctg
     4561 caaaacgctc ccatccggtc aggcgcagtt ctgccacgag gtcaagataa gtaggcagtt
     4621 cgctgcgggt gccggagaag aagacaaatg gcggccgcac cattgccatc aggcgcagaa
     4681 actctaccat gccgaagtag ttcatcgcgt ccccttttcc ccggcggcgc ggctttggtt
     4741 gaaccgcgcg tcgtgctgcc tgcctttcag ttcgctgata tacgcctgcc ggcagtgttg
     4801 gcggtcgaaa aatacgccgt tgatcagggt gtataaaacc ttccagcggg ttttgggctt
     4861 ttgggcgagc cgcgcgccgc gataggtgcg gcttgagagg gtttcgtccg ccgcgccgcc
     4921 ggttagggcg ttgaacagtt ggtcgatggc taccaacagg tggtaaacgt attttttcag
     4981 ccgatgttta gcgtccattc ttcgatttcc ttttccagcg cgtccaatcc tggcgcggtt
     5041 tcgatggtgt tcaatttgtc ttcgagctgc tgccgctttc cgataatcgc gccggcggca
     5101 acggccaggc gggcggattt ttcgacaact ttttcaatca aaacgtccaa ttccacgccc
     5161 cttgcggcgg cgatttgcgc cagcatcggg gtcggggcgt tgttgtccgc ctaccgcgcg
     5221 agggcttctt tttcctgcct gtaaaagctg tcgatttcca cttggggata gcccgccaag
     5281 aggctgtttt tgagttcgtc cgcctttgcc gcgaggcgga atgccgtggc ggttttttgt
     5341 tcggcgaaac gggcggcggc agcggcttcg ccgatttccc atttcttgcc gtcccattcg
     5401 tggtattcgg acgggcgcgg cggggttaaa acggggcggc cgtcggaatc tgcggcaatc
     5461 tgcccgccct gcgcctgtcc tgccaaaagg gcggcgtatt cttcggcgcg gacggcaacc
     5521 gcgccttcgg ggatgctgcc caaggtgtcg tcgtaaaagc cgtttttgaa atagatggtc
     5581 atttcggggt ttcctttttt gtatttacat tttggttttt tgccgcttat aatgtaacga
     5641 caaaatacgc tgcaagctgc aatgaagatt gaatttgact ccgaaaaaaa tcaacgcaat
     5701 atcgaagagc ggaatttgcc ctttgaatca gtcgggcaaa tacgctggac aacggccgtt
     5761 attgtgcccg atgtctgctt tgattacccc gaaccgcgtt atgttgccgc tgcttatcta
     5821 ggcgatacgc aacgcctgca cattgtctgt tttacgccga taaaagacgg catccgtgtc
     5881 atcagttttc gcaaagcgaa taaaagagag gttaaaaaat atgcaacgtc aatccttaac
     5941 aaacgctgac ggcgaagtgc gggaacttgc cgccgaagat tttgcgcttg cccgccccat
     6001 tgccgaagcc ctgcccgaag acttggcaca ggtgcttttt tcccatcaaa aacaattgga
     6061 agaaaagggc gtaatgcaaa aacgaaacac aggcaaaacc ccgaaacaac tcgttaccat
     6121 acgcctgtcc gccgatgtgg tcgaaaaatt ccgcgccggc ggcaagggct ggcagacgcg
     6181 catcaacgag gttttgcgcc aatatgtcgc ccaattgaaa taacgggggc ggccgctgct
     6241 tatcgggcgc gcccgactat cctttcccgc gcggtttcgg ccgtgcggtt
//
LOCUS       DAWHXZ010000118         6252 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000118 DAWHXZ010000000
VERSION     DAWHXZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.118"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..218)
                     /locus_tag="WIE35_001409"
     CDS             complement(<1..218)
                     /locus_tag="WIE35_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ4154318.1"
                     /translation="MASSGVNNEIKDKKLSLWAKRQDGSVKWFCGQPVTRADTDTDTA
                     VTAASDKQIDPKHLPSTCRDESTAGCTN"
     gene            complement(247..576)
                     /locus_tag="WIE35_001410"
     CDS             complement(247..576)
                     /locus_tag="WIE35_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ4154319.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLAIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(654..872)
                     /locus_tag="WIE35_001411"
     CDS             complement(654..872)
                     /locus_tag="WIE35_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ4154320.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVQQTQPFQLHRCS"
     gene            994..1947
                     /locus_tag="WIE35_001412"
     CDS             994..1947
                     /locus_tag="WIE35_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ4154321.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIIITNKVMISADIIAHAPQLELIAVSATGVNNVDIEAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLARYAQAFGMRVVFAEHKHAPVVREGYVSFEDAVQTADVLSLHCPLNAQTENMIGEN
                     ELQQMKPGAVLINCGRGGLVDENALVAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(2022..4079)
                     /gene="metG"
                     /locus_tag="WIE35_001413"
     CDS             complement(2022..4079)
                     /gene="metG"
                     /locus_tag="WIE35_001413"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ4154322.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFNIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLDECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVSGSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPILPKVAEN
                     AAKFLNLEAITWANTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EESQYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYLNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(4194..6032)
                     /gene="glmS"
                     /locus_tag="WIE35_001414"
     CDS             complement(4194..6032)
                     /gene="glmS"
                     /locus_tag="WIE35_001414"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ4154323.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPTDVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGKLSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
BASE COUNT         1247 a         1510 c         1888 g         1607 t
ORIGIN      
        1 tggtttgtgc aaccggcagt tgattcatcg cggcaggttg acggcaggtg cttggggtcg
       61 atctgcttgt cgctggcggc ggtaacggcg gtgtcggtgt cggtgtcggc gcgcgtaacc
      121 ggctgtccgc agaaccattt taccgaaccg tcttgacgct tggcccacag ggagagtttt
      181 ttgtctttga tttcgttgtt tacgccgctt gaagccatta tgtcagacgg tattgcccgg
      241 gcagctttat tcgtacactt tcagcagctc gacttcaaat atcaaagtgg cgtgcggggg
      301 aatcacgccg cccgcgccgt gtgcgccgta gcccatttcc gaagggatgg ccagcttgcg
      361 tttgccgcct tccttcatgc cgccgaagcc ttcgtcccag cctttgatga cttgtccgac
      421 accgagcgtg atggtcagcg gctggcggcg gtcgaggctg gagtcgaatt tggttccgtt
      481 ttccagccaa cctgtgtaat gcacggtaat ctctttgcct ttaactgctt cttttccgaa
      541 gccttcttgc aagtcttcaa taatcaggcc gcccatattt gtcctttcgt tgcttgttgg
      601 tcaaaacggc aagggtaaca taccgtccgt cgaagtcaaa tgccgctcaa acgtcagctg
      661 catcggtgca gctgaaacgg ctgtgtttgt tggactgttt tatttttttc gtaaaggttc
      721 catgcttttt catggaaata gaaaacgacg gtgttgatta ggggctcgac cagcgcgacc
      781 gcccccgata cgcctatact gcccgtcagt acataggtta cactgaaggc ggtgctgaaa
      841 tgcagtgcgg caaaagtcag ggttttaagc atcatcctct cccgaattgg acattgacgg
      901 aggaatgata aaggttatca taaggttgcg cggtttaaat ttgctatttg ttgttagtgt
      961 aaataaatca tttttcaaat ggggatagaa attatgaatc ataaaaagat cgttgttttg
     1021 gatgcggata ctttacccgg ccgggttttt cattttgatt ttccgcacga gcttgcggtt
     1081 tacggtacga caggtgcgga tgaaacggca gaacgggtgc gcgacgcgca tattatcatc
     1141 accaataaag tgatgatttc agcggatatc attgcacacg ctccgcaact tgaattgatt
     1201 gccgtcagtg cgaccggtgt gaacaatgtc gatattgagg cggcgaaggc ggcgggcgtt
     1261 gcggtatgca atgtccgcgc ctacggaaac gaatcggttg cggaacacgc ctttatgctg
     1321 atgattgcgt taatgcggaa tttgcccgcc tatcagcgtg atgttgcggc aggattatgg
     1381 gaaaagtcgc cgtttttctg ccattatggc gcgccgattc gggatttgaa cggtaaaacg
     1441 ctggcggttt tcggacgcgg caatatagga cggacgcttg cccgatatgc acaggcattc
     1501 ggtatgaggg tagtgtttgc cgaacacaaa cacgcgcccg ttgtgcgtga aggctatgtt
     1561 tcctttgaag atgcggtaca gactgccgac gtgttgtcgc tgcactgtcc gctgaacgcc
     1621 caaactgaaa atatgatagg cgaaaacgaa ttacagcaga tgaagcccgg tgcggttttg
     1681 atcaattgcg ggcgcggtgg gctggtggat gaaaatgcgc tggttgccgc actcaaatac
     1741 gggcagattg gcggggcagg tgtcgatgtt ttgacggagg aaccgcctag gggcggcaat
     1801 cccttgctga atgcacgact tcccaatctg attgttacgc cgcataccgc gtgggcaagt
     1861 cgtgaagcgt tggacaggct gttcgatata ttgttggcga acattcacgc ctttgtgaaa
     1921 ggcgaggcgc aaaaccgcgt ggtttgaacc tgtcgggatt gcggaaaaaa atgccgtctg
     1981 aacgcctcaa gggttcagac ggcatttttt gagatttctg cttaaccgac tttgtcgccc
     2041 ggttgcgcgc ctgcatccac atccaagagc ttcagtttcc cgtctgccgt ggcggcagag
     2101 agaatcatgc cttcggatac gccgaatttt gccattttgc gcggggcgaa gttggcgacg
     2161 gcgatgacca tccttccgtt taactcggca gggttcaggt aagacgcggc gatgccggag
     2221 aagatgatgc gtttttcaaa accgaaatcg aggtcgaatt tcaaaagttt ggtgctgcct
     2281 tcgacagctt cgcagttcaa tactttggca acgcgcatat cgattttcat aaagtcgtcg
     2341 aagcttgcct gttcggcaac tttttcgtat tgactttctt cggcggcagg tgcggctgcg
     2401 gcggcgatgc tttgtttgtt ggcttcgatt aaatcgtcca cttgtttttg ctccactcgt
     2461 tgcatgagat gttcgtattt gttgatggcg tgttcgccca aggtttcgcg tgtgtttgcc
     2521 caagtgatgg cttccaaatt caggaatttg gcggcgtttt cggccacttt cggcaagatg
     2581 ggggcaaggt aggcggtcag catggtaaag gcgttgatga gttcgctgca tacttcgtgc
     2641 aggcgttcgt cttggccttc ttgtttcgcc agttcccaag gtttgttggc atcgacgtat
     2701 tcgttgacaa tgtctgccaa ggccatgatg tcgcgcaggg cgcgggcgta ttcgcggttt
     2761 tcgtattgct cggcgatggt gtcgctttcg gcggcaagtt ttgccagcaa tgcgctgcct
     2821 gaaacgtctt tcagacggcc ttcaaagcgt ttggcgatga aacctgatgc gcgggcggcg
     2881 atgttgacgt atttgccgac gaggtcgctg tttacgcgcg aaataaagtc ttgcaggttc
     2941 aaatcgatgt cttcgatttt gctgttgagt tttgcggcga tgtagtagcg catccactcg
     3001 gggttcaggc cttgttccaa ataagatttg gcagtgatga aggtgccgcg cgatttggac
     3061 attttttgtc cgtctacggt caaaaagccg tgtgcgtaca cgccggtcgg ggcgcggtgg
     3121 ccggagaaat gcagcatggc aggccagaac agggcgtgga aatagaggat gtctttgccg
     3181 atgaagtggt acatctcggt ttggctgtcg gctttgaagt attcgtcaaa atcgacgccg
     3241 atgcggtcgc acaggttttt aaacgacgcc atgtagccga cgggcgcgtc cagccagacg
     3301 tagaagtatt tgcccggcgc gtcggggatt tcaaaaccga aatacggtgc gtcgcgggaa
     3361 atgtcccagt cggacagggt ggtttcttcg ccttcgccca gccattcttt cattttgttg
     3421 agggcttcgg gttgcagatg gggcttgccg tcgtgcggat tgttgccgga agtccatgct
     3481 ttgaggaagt cggcgcattc gtccagtttg aagaagaagt gttcggattc gcgcaattcg
     3541 ggtttggcac cggaaacggc ggaatacgga ttaatcagtt cggtcgggga ataggtcgtg
     3601 ccgcagactt cgcagttgtc gccgtattgg tcttgggcgt ggcatttcgg gcattcgcct
     3661 ttaacgaaac ggtcgggcag gaacatttgt ttttcagggt cgaaaagctg ctcgatgacg
     3721 cggctttcga ttttgccgtt ggctttcagc gcgcggtaaa tgtcttggga aaactgtttg
     3781 ttttcagggg aatgggtgct gtaataattg tcgtagccga tgttgaaacc ggtaaagtcg
     3841 gcgaggtgct cttcgcgcac tttggcaatc atgtcttcgg gcgcgatgcc ttgtttttgc
     3901 gcggcaagca tcacgggcgt gccgtgggtg tcgtcggcgc agcagtagta gcattcgtgg
     3961 ccgcgcagtt tttggaagcg cacccaaacg tcggtttgga tgtgttcgac catgtggccg
     4021 aggtggatgc tgccgttggc atagggcagg gcggaggtaa ctaagatttt gcgtgtcata
     4081 ttgtgctttg caaacaatgg gtaaaggcgg attataccgc aaatcaaacg gggaaatgcc
     4141 gtctgaagcc tgaaaaatcg ggcttcagac ggcattttgc caatcggcgg gatttattcg
     4201 acggttacgg atttcgccag attgcgcggc ttgtccacat ccgtgccgcg tgcgagggcg
     4261 gtgtggtagg cgaggagctg cacggggata gtatgcacga cgggggacag tttgccgacg
     4321 tggcgcggtg cgcggataac gtgcacacct tcggtggcat taaaattgct gtcgaggtcg
     4381 gcaaagacga aaagttcgcc gccgcgcgcg ccgacttcct gcatattggc tttgactttg
     4441 tccaacaggc tgtcgttggg tgcgatgacg acgacgggca tgttttcgtc caccaaggca
     4501 agcggcccgt gcttcagttc gccggcagga taggcttcgg cgtggatgta ggtgatttcc
     4561 ttcagtttta acgcgccttc gagggcaatc gggtaatgga tgccgcgccc caaaaacagc
     4621 gcgctggttt tcttggcaaa ctgttgcgcc caagcggcaa tttgcggttc gaggtttaaa
     4681 gcgtgctgca cgctgccggg aagctggcgg agttcttcgg tataacgtgc ttcatcttcg
     4741 ccggatacca agccgcgtac cttcgccagc gttaccgcca aaccgaacag tgcgaccagc
     4801 tgcgtggtaa atgctttggt cgaggcgacg ccgatttccg caccggcacg ggtataaagc
     4861 acgaggctgc tttcgcgcgg cagggcggat tccatcacgt tgcaaatgga gaggctgtgg
     4921 cggtgtccca aagatttggc gtatttcaaa gcctccatcg tgtccagcgt ttcgccggat
     4981 tgggaaatgg taatgaccag ttggtccgga tcggcaatca cgctgcggta tcggtattcg
     5041 ctggcgattt cgacgtcggt cggaattttg gcgatggatt cgagccagta tttggcggtc
     5101 agcgcggcgt aataggacgt gccgcaggca aggattttga cgctgcggat gctttcaaac
     5161 acgcttttgg cgtttttgcc gaagttttcg gggatgaagc cgccgtcgag gaaaacctcc
     5221 gccgtgtctg caatcgcgcg gggctgctcg tggatttcct tttgcataaa gtggctgtac
     5281 ggccccagct ccaaagaggc gagcgagagt tcggatacct tgactttgcg ttcggcaggc
     5341 aggccgtttt tatcggtcag ccttttgatg ccgtctgaag ccagcagtgc gatgtcgccg
     5401 tcttcaaggt acgccacgcg gcgcgtaaag gcgatgacgg cggacacgtc cgaagcgata
     5461 aaggtttcat cgtcgcccaa agcgaccaaa agcgggcagc ccatacgcgc cacaactaat
     5521 tcatcaggct tgtcttgggc aataaccgcg atggcgtatg cgccgtggaa acgtttgacc
     5581 gcttcctgca cggcttcaaa cagcctgccg ccgttttgcg cgtattcgtg attgatgctg
     5641 tgggcgatga cttcggtatc cgtttgcgat tcaaaccggt atcccaaacc ttccaagcgt
     5701 ttgcgttcgc tttcaaagtt ttcgatgatg ccgttgtgga cgacggctat catcccaccg
     5761 gaaatatgcg gatgggcatt cggctcggta acgccgccgt gtgtcgccca acgcgtatgc
     5821 ccgatgccga tgccgccgct gatgcctttt tcgcgtgctg cgtcttccat aagctgcacg
     5881 cgtccgacgc ggcgcacgcg tttgattttg ccgtcggtgt tgacggcaat gcctgatgag
     5941 tcataacccc ggtattcgag gcgtttgagg ccgtcggtca gaaaatcgac gacgttgtga
     6001 tgggcgcgga tggcgccgac gataccgcac ataactgttc cttagtatcc ggttgaaaaa
     6061 aaacaggcgc ggacggctcc cgtgccgcac cttcctcttc ggattataaa ccgcctcccg
     6121 cgccgaaaaa cagcaaaatg ccgtctgaag gcttgggctt gctcaaaaaa gggagggatt
     6181 tccccgcctg accgggatgg gcgttcagac ggcattgcct gccgccggtt tatagttttt
     6241 acaaatcaat at
//
LOCUS       DAWHXZ010000119         6225 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000119 DAWHXZ010000000
VERSION     DAWHXZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.119"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..109
                     /locus_tag="WIE35_001415"
     CDS             <1..109
                     /locus_tag="WIE35_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ4154324.1"
                     /translation="KDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            162..740
                     /gene="pth"
                     /locus_tag="WIE35_001416"
     CDS             162..740
                     /gene="pth"
                     /locus_tag="WIE35_001416"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ4154325.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            801..1007
                     /locus_tag="WIE35_001417"
     CDS             801..1007
                     /locus_tag="WIE35_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ4154326.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            1004..1366
                     /locus_tag="WIE35_001418"
     CDS             1004..1366
                     /locus_tag="WIE35_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ4154327.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            1597..3012
                     /locus_tag="WIE35_001419"
     CDS             1597..3012
                     /locus_tag="WIE35_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEZ4154328.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            3131..3640
                     /locus_tag="WIE35_001420"
     CDS             3131..3640
                     /locus_tag="WIE35_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ4154329.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKNGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            3807..5189
                     /locus_tag="WIE35_001421"
     CDS             3807..5189
                     /locus_tag="WIE35_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ4154330.1"
                     /translation="MASIARDIFKAYDIRGIVGKTLTDEAAYLIGKAIAAKAAEKGIT
                     RIALGRDGRLSGPELMEHIRRGFTDSGINVLNVGMVATPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLSIIEKDGFAAAGKQGSVTEKDISGEYLKHI
                     TGHIRLKRPMNIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGTFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSAMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(5209..5964)
                     /locus_tag="WIE35_001422"
     CDS             complement(5209..5964)
                     /locus_tag="WIE35_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ4154331.1"
                     /translation="MIKIRNIHKTFGENTILRGINLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(5974..>6225)
                     /locus_tag="WIE35_001423"
     CDS             complement(5974..>6225)
                     /locus_tag="WIE35_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ4154332.1"
                     /translation="APQAFRVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANR
                     TYDFLPVYIEAALVYWCFCKVLFLIQARLEKRFDRYVAK"
BASE COUNT         1527 a         1864 c         1472 g         1362 t
ORIGIN      
        1 caaagacgac acgcccctgc gcgacggcga ccgcatcgaa gtgtaccgcc cgctgttgat
       61 cgaccccaaa gaagcgcgcc gcaaacgcgt tcaaaatcaa gaagaataac catgccgtct
      121 gaagccttca gacagcattc gacagaaaca cggaaaatat catgtcaaac aaaatcaaaa
      181 tgcttgtcgg attgggcaac cccggcaaag aatacgaaca aacccgacac aatgcgggtt
      241 tttggttcct cgacgaactg gcgtggaaat ggaaggcttc atttaaagaa gaaaaaaaat
      301 tcttcggcga agtcgcccgt gccgccctgc ccgacggcga cgtttggctg ctcaaacctg
      361 ccacgttcat gaaccgttcc ggacaggcag ttgccgcgct tgcacagttc tacaaaatca
      421 aacccgaaga aatcctcgtc gtccacgacg aactcgacat tccctgcgga cggattaaat
      481 tcaaactcgg cggcggcaac ggcggacaca acggcttgaa agacattcag gcaaaactcg
      541 gcacggcaga ctattaccgc ctgcgcctcg gtatcgacca cccgggcgac cgcaacctcg
      601 tcgtcggcta tgtcctgaac aaacccagta cggaacaccg ccgacagatt gacgatgccg
      661 tcgccaaatc cctgcaagcc atacccgaca tccttgccgg caaatgggaa gaagcaaccc
      721 gcttcctgca cagcaaatga cccgatgccg tctgaagccc tttcagacgg catgttcccg
      781 atttccatat ccgaacagtc atgaccgaac tcaagcagct tatccaaacc gaatccatcc
      841 ccgtcatcga agaaaccctc gatttcctgc tgtacgaatg cagcatcgac gacgcaccat
      901 ccgccgaaga agtggcacaa tggcgcgaca tacttgccgc acgcggcggc aaattcctgc
      961 gcctgtccaa aatctgccaa acgtggctgg acgaggaggc ggcatgaagc tgccgcgcaa
     1021 ccgcttcagc ctgctttccg cattgtggtt tgccggcggc atctattcgc tgctcttcaa
     1081 agctgccgac accgcgccgc cgccgtttcc gcatttcgac aaagcagcac accttgccct
     1141 gtttttcgca caaatctggc ttttgaccaa agcattcaaa accggaaaac ttcccatccc
     1201 ctaccgcagc ctgatggtct ttgccctctg tttcgccctc ttcagcgaat gcgcgcaggc
     1261 atggtttacc gcaacgagaa ccggcagttt gggcgatgtt cttgccgata tggcaggtac
     1321 ggttctcgca ctctttgccg cccgcgccgc cgaccgcccg gactgaatcg gtttatttcc
     1381 cccaaacagg atgcacctct tcccgataca tcctattttt ccccttacat atcaatccca
     1441 tctcataaac aaaaccgaca aatcgtttac aatatattta cactacatca gattacaaat
     1501 atactcgaac cagttgtaga atcggcggtt ccacaaaccg tttcggattg cacgacacaa
     1561 aagaaaaacc gattttattg ccttaaagga gtattcatga acctgcatgc aaaggacaaa
     1621 acccagcatc ccgaaaacgt cgagctgctc agtgcgcaga agccgattac cgactttaag
     1681 ggcctgctga ccaccattat ttccgccgtc gtctgtttcg gcatttacca catcctgcct
     1741 tacagccccg atgccaataa aggtatcgcg ctgctgattt tcgttgccgc actttggttt
     1801 accgaagccg tccacattac cgtaaccgca ctgatggtgc cgattctcgc cgtcgtactc
     1861 ggtttccccg acatggacat caaaaaggcg atggctgatt tttccaaccc gattatctac
     1921 atttttttcg gaggcttcgc gcttgccacc gccctgcata tgcagcggct ggaccgtaaa
     1981 atcgccgtca gcctgttgcg cctgtcgcgc ggcaatatga aagtggcggt tttgatgttg
     2041 ttcctcgtta ccgcctttct gtccatgtgg atcagcaaca ccgccaccgc cgcgatgatg
     2101 ctgcctctag caatgggtat gctgagccac ctcgaccagg aaaaagaaca caaaacctac
     2161 gtcttcctcc tgctcggcat cgcctattgc gccagcatcg gcggcttggg cacgctcgtc
     2221 ggctcgccgc ccaacctgat tgccgccaaa gccctaaatc tggacttcgt cggctggatg
     2281 aagctcggcc tgccgatgat gctgttgatt ctgcccttga tgctgctctc cctgtacgtc
     2341 atcctcaaac ctaatttgaa cgaacgcgtg gaaatcaaag ccgaatccat cccttggacg
     2401 ctgcaccgcg tgatcgcgct gttgattttc cttgccacag ccgccgcgtg gatattcagc
     2461 tccaaaatca aaaccgcctt cggcatttcc aatcccgaca ccgttatcgc cctgagtgcc
     2521 gccgtcgccg tcgtcgtctt cggcgtggcg caatggaagg aagtcgcccg caataccgac
     2581 tggggcgtgt tgatgctctt cggcggcggc atcagcctga gcacgctgtt gaaaacatcc
     2641 ggcgcgtccg aagccttggg acagcaggtt gccgccacct tttccggcgc gcccgcattt
     2701 ttggtgatac tcatcgtcgc cgccttcatt atttttctga ccgagttcac cagcaacacc
     2761 gcctccgccg cattgcttgt accgattttc tccggcatcg ctatgcagat ggggctgccc
     2821 gaacaagtct tggtattcgt catcggcatc ggcgcatctt gtgccttcat gctgccggtt
     2881 gccacaccgc ctaacgcgat tgtgttcggc acgggcttaa tcaagcaacg cgaaatgatg
     2941 aatgtcggca tactgctgaa catcctctgc gtagtattgg ttgctctgtg ggcttatgct
     3001 gtactgatgt aaaccatcga cctaaacaac aagaccgtct gaaagaatat ttttcagacg
     3061 accttgaagt ttgtcgttca gacacaattt gtcgaatcat tcaaaaccgg attctaacga
     3121 aaggaaaccc atgattatcc tgcacaccaa caaaggcgac atcaaaatcg aactcgattt
     3181 cgacaaagcc cccgttaccg ccaaaaactt cgagcaatac gtcaaaaacg gcttctacga
     3241 cggcgtaatc ttccaccgcg tcatcaaggg cttcatgatt caaggcggcg gcatggatga
     3301 aaacatgaac gaaaaagaaa cccgcgatcc gattcaaaac gaagcgtcca acggcctgcc
     3361 caacgataaa tacaccatcg ccatggcgcg tacttccgat ccccattccg ccagcgcgca
     3421 attcttcatc aacactgccg acaacgcttt cctgaacttc cgttctaaag agctgtacgg
     3481 caaaaccgtc gtccaagact ggggctatgc cgtattcggt aaagtcgttg acggttttga
     3541 cgttgtcgat gccatcgaag gcgtttctac caaacgtcat ggttaccacg acgacgtacc
     3601 gagcgaacct gtcgtcatca ttaaagccga agcggtataa accgacaatc cgaaagcagc
     3661 ctgcacaaag gctgctttta tttcagacgg catgttattt gatgtaaccg tgctatgccg
     3721 tctgaaaacc ggacgcaagc cttcagacgg cataccgcta tgttaaaata tgcccgtttt
     3781 tcctaaacgg acacaaagga aaccttatgg caagcatcgc ccgcgacatc ttcaaagcct
     3841 acgacatccg gggtattgtc ggcaaaaccc tgaccgacga agccgcctac cttatcggca
     3901 aagccatcgc cgccaaagcc gccgaaaaag gcattacccg catcgcgctc ggacgcgacg
     3961 gacgcttgag cggtcccgaa ctgatggaac acatccggcg cggctttacc gacagcggca
     4021 tcaatgtcct caatgtcggt atggttgcca ctcctatgct ctacttcgcc gccgtcaacg
     4081 aatgcggcgg cagcggcgtg atgattaccg gcagccacaa tccgcccgat tacaacggct
     4141 ttaaaatgat gctcggcggc gacacgcttg ccggcgaagc catccaagaa cttttgtcca
     4201 tcattgaaaa agacggtttt gctgccgccg gcaaacaagg cagcgtcacc gaaaaagaca
     4261 tctccggcga atacctcaaa cacattaccg gacacatcag gctcaaacgc ccgatgaaca
     4321 tcgccattga cgcgggcaac ggcgtgggcg gcgcgtttgc cggcaaactc tacaaaggct
     4381 tgggcaacga agtaaccgag cttttctgcg acgtggacgg cactttcccc aaccaccatc
     4441 ccgacccatc caaaccgaaa aacctgcaag atttgattgc cgcgctgaaa aacggtgatg
     4501 ccgaaatcgg cttggcgttt gacggcgatg ccgaccgctt gggtgtggtc accaaagacg
     4561 gcaacattat ttatcccgac cgccaactga tgcttttcgc gcaagacgtg ttgagccgca
     4621 atcctaaagc caaagtgatt ttcgacgtca aatccacccg tctgcttgcg ccttggatta
     4681 aagaacacgg cggcgaacct gtgatggaaa aaaccggcca cagctttatc aaatccgcca
     4741 tgaaaaaaac cggcgcgccg gttgccggcg aaatgagcgg acacatcttc ttcaaagaac
     4801 gctggttcgg cttcgacgac ggtctgtacg ccggcgcacg cctcttggaa atcctgtctg
     4861 cctccgataa tccgtccgaa gtgttaaaca acctgccgca aagcatttcc acgcccgaac
     4921 tcaacatcgc cctgcccgaa ggcagcaacg gccatcaggt tatcgacgaa ctcgccgcca
     4981 aagccgaatt tgaaggcgca accgaaatca tcaccatcga cggtctgcgc gttgaatttc
     5041 ccgacggctt cggtctgatg cgtgcttcca ataccacgcc gattctggtg ttgcgttttg
     5101 aggcggatac gcaagaagcc atcgagcgga ttcaaaacca attcaaagcc gtcatcgaaa
     5161 gcaatccgaa tctaatctgg cctctgtaaa cacaggaaaa atgcttaatc aaatcttggt
     5221 agattggatt tggcttaaaa atctccgcgt ccgttcgtgt ttggggtggt cgaacaactc
     5281 tttcgggctg ccctgctcta cgataacgcc gccgtccatc acgacaacgg tagtcgcaac
     5341 ttccagcgcg aacttgattt cgtgggtaac gacgaccatc gtccaacctt cccgcgccaa
     5401 ttccttcatg gcgttcaaca cgtcttgcac caactcgggg tcaagcgcgg aagtcggttc
     5461 gtcaaacagc atcagctcgg gctgaatcgc caatgctcgg gcaatgccga cgcgctgctg
     5521 ctgaccgccg gaaagctggt agggatacaa atccactttg tcgcccaagc cgactttttc
     5581 cagcagtttc agagcctctt cgcgcgcttg ggcggcaggc ttgccctgta cggcaaccgg
     5641 tccttccatc acgttttcca aggcggtttt gtgcggaaag aggttgtatt gttgaaacac
     5701 catgcctgat ttgcggcgca gtgccaaaat atcgtgtttg cttggttttt tagaaaaatc
     5761 gattttcagc ggtcgctcgt tgtcgaactc gatttgtccg tcttcgggca tttccaacgc
     5821 gtttaggcat cgcagaaacg tcgttttgcc tgagccggaa ggcccgagga tgacgaccac
     5881 ctgccctttg cacacatcca aattgatgcc gcgcaaaatg gtattttcgc cgaaggtctt
     5941 atggatattg cggattttaa tcatgacaac tccttatttg gcgacatagc ggtcgaagcg
     6001 tttttccaag cgcgcctgaa tcaggaacag cactttgcaa aaacaccagt aaaccaaagc
     6061 ggcttcgata tagacgggca aaaagtcata agtgcggttt gccgtttcct gcgcgacgcg
     6121 gaataattcc gttaccgtca cgactgccgc gagcgaggtg tttttaaaca aaccgataaa
     6181 ctcgttgctc aaaggcggca cggcaacgcg aaatgcctgc ggcgc
//
LOCUS       DAWHXZ010000120         6210 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000120 DAWHXZ010000000
VERSION     DAWHXZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.120"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            141..1538
                     /gene="aspA"
                     /locus_tag="WIE35_001424"
     CDS             141..1538
                     /gene="aspA"
                     /locus_tag="WIE35_001424"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ4154333.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEVLGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLAVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1610..2485)
                     /locus_tag="WIE35_001425"
     CDS             complement(1610..2485)
                     /locus_tag="WIE35_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ4154334.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(2487..3209)
                     /locus_tag="WIE35_001426"
     CDS             complement(2487..3209)
                     /locus_tag="WIE35_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ4154335.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3231..3710)
                     /gene="ybaK"
                     /locus_tag="WIE35_001427"
     CDS             complement(3231..3710)
                     /gene="ybaK"
                     /locus_tag="WIE35_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ4154336.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGNGLIVLMHGDKQISTRNLARHLGAKRIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3785..4147
                     /locus_tag="WIE35_001428"
     CDS             3785..4147
                     /locus_tag="WIE35_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ4154337.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            4182..5045
                     /locus_tag="WIE35_001429"
     CDS             4182..5045
                     /locus_tag="WIE35_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ4154338.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKVVFEILEDYAVGAEL
                     EASAEPLAAQEPSLVFTAECVSDGICSVVLPHRGILHIAPETALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEEAGIVLDSVQDSENFTALTVIKFSAAQKELTVPNGGIFKA
                     VHLFFKTENAE"
     gene            5138..6097
                     /gene="ttcA"
                     /locus_tag="WIE35_001430"
     CDS             5138..6097
                     /gene="ttcA"
                     /locus_tag="WIE35_001430"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ4154339.1"
                     /translation="MSKKIKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQSLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
BASE COUNT         1574 a         1794 c         1515 g         1327 t
ORIGIN      
        1 ccgaaaacaa cgtttcccgt tttcagacgg catacgatat ggacaatttc atccggccgg
       61 tatagtgttc gtcctgcggc ggcatctttc cgatggcgca ttttttcaat attaattatc
      121 cgacttacaa aggtaaaaac atgactgtcc gtatcgaaca cgatttattg ggcgaccgcg
      181 agattcccgc cgaagtgtat tggggcatcc acaccctgcg cgccattgaa aactttaaaa
      241 tctccacaca aaaaatttct gacgtgccgc agtttgtccg cagtatggtg atggtgaaaa
      301 aggcgacggc acaggcaaac ggtaagttgg gcgcggtcaa gcccgaaatc gccgccgcca
      361 ttgaaaaggc ttgcgacgaa gttctgctga acaaccgctg cctcgaccaa ttcccgtccg
      421 atgtgtatca gggcggtgcg ggtacttcgg tcaatatgaa caccaacgaa gtcatcgcca
      481 acctcgcatt ggaagtcttg ggatacgaaa aaggccgcta cgatatcgtc aatccgatgg
      541 atcatgtcaa cgccagccaa tccaccaacg atgcctaccc taccggcttc cgccttgccg
      601 tgtattacag catcggcgaa ttgctcgaca aactggccgt cctgaaaaac gcctttgccg
      661 ccaaagccga agagtttaaa gacgttttga aaatgggccg cacccagctt caagatgccg
      721 tgccgatgac tgccggccaa gaattccaat ctttccaagt gttgttggaa gaagaaatcc
      781 tcaacctcga ccgcacccgc caactgctgt tggaagtcaa tttgggcgca acggcaatcg
      841 gcacgggcgt gaacacgccc aaaggctacg ccgaactggt ggtcaaaaaa ctctccgaag
      901 tcagcggctt gccttgcaaa ctgaccgaaa acctgattga ggcgacctcc gactgcggcg
      961 catatgtgat ggtacacggc gcattgaagc gcacggcggt caaactctcc aaaatctgca
     1021 acgacttgcg cctgctctct tccggcccgc gtgccggctt gaaagaaatc aacctgcccg
     1081 aattgcaggc cggttcttcc ataatgcccg ccaaagtcaa tcccgtgatt cccgaagtgg
     1141 tcaaccaagt ctgcttcaaa gtcatcggca acgacacgac gattaccttc gccgccgaag
     1201 ccgggcaact gcaattaaac gtgatggagc cggtcatcgc ccaatgtatg tttgaaacca
     1261 tttccctctt gggcaatgcc gcagtcaacc tgtccgacaa atgcgtcaaa ggcattacgg
     1321 tcaaccgcga aatctgcgaa cgttacgttt tcaactccat cggtttggtg acttatctga
     1381 atccgtatat cggacaccac aacggcgatt tggtcggcaa aatctgcgcc caaaccggca
     1441 aaggcgtgcg cgaggttgtc ttggaacgcg ggctgttgag cgaagaagaa ctcaaccgca
     1501 tcctctctcc cgagaacctg atgaaccccc atctgtaacg ttgcagacaa atgccgtctg
     1561 aagcagggat gctgcttcag acggcatttt gttatatcaa tcagccggat tatatgccga
     1621 acagtacctt gacttccccc caataaaaca ggcaggcgat agaacacagt gccaacacga
     1681 ttcccgccgt attgattgtg ttgatttttt ccttaaagaa caatgcgccc gaaagcgtac
     1741 ccaaaacaat cacgccgata ttcataccgg caaaaaccaa ggtcggatta tccttcatca
     1801 tttggtgcgc ggtgatgtag gttacgatat tcataaaatt caaaccgccc aagaatatgc
     1861 cgccgaccac actctcaaca cgccatctga ccgatttggc aaacaggcag gcaaacatca
     1921 gcacacccgc cagcacaaat gcaaccagca ggttgcccgc aaatgccgtt ccgcttttgg
     1981 caagctgttt gaacaggata tcgataatgc cgtaacctgc ccacacgccc agcagcaatg
     2041 ccgcctgccg ccacgcgctt cctgattttt tgccaccgct gtgtttccaa agcaggcaga
     2101 acagtgcggc aaatgcgagg cacagcccga ttagtctgcc ttcgctgagt ttttcgccaa
     2161 acagcgtcaa ggcggcaaca atcggcaaaa acagcgacaa acgctgcgcc gcgtcggatt
     2221 tgacgatacc ggcggattct acggatttgc ccataatgac aaatacggac ggcagcagca
     2281 cgcccaatgc ggcaaacagc caccatgtcg gcaaaaatgc gccgatattg ccgacatccg
     2341 gcttcaatac cagcagggtc agtatgaccg cgaccacata attgacggcg accgcctgcg
     2401 cgatgtcgat ttttttcttc cttgccattt tcagcaaaat ggaaaccgac acgctgaaca
     2461 cgatgctgat taacaggtaa gccatactat atccatttct cgggtgcaac cgccctgccg
     2521 ccgttgaagc ggataaagcc ggcgatgatg cggtagaaat accagacgtt cgttgcaata
     2581 ataatcagca cgccgatacc caaaagggca gtcagcgcac ccaaaatata aagccaaaat
     2641 gtgcgccaaa aggttttaat caggtattcg gtatgtgcgg catagacaat gctgtccaaa
     2701 ctgttccgtt tcatataggc gagaatcacg ccgactatgg gcatataggc gaaaatcacg
     2761 ccgactatgg gcatgaacag catgattgca cccaaaccga acaggatgta ggcggcgagg
     2821 acatagttgc gcccgcttcc gccgccggac ggttcgtaag tatggcacga acgtgatgcc
     2881 tgccgttcaa acgcctgtct gaacgcctgt tcgcggcgca tctgttcgcg gtaaaactgc
     2941 tccctgcgcg cctgttcgcg gcggaatgcc tcttcctgcc gcccgcgttc ctcatgccgt
     3001 ctgaaggacg cgtcgtattg catccgtttc gacaggtcgg aaagcgtatc gtaagcccgt
     3061 tggatttctt tgaacttttc ttccgcctgc ggattgcccg gattgcggtc gggatggtat
     3121 ttcattgcca gcttgcggta ggcgcgtttg atttcgtccg cccccgcctg cggcgacacg
     3181 cccaatacgg catacaaatc tttgtccata ttgttcctta tacttcccgg tcaaaccgcc
     3241 gcctgtattg ttttcgggtt caaaatattt aaatctccgg gacggatacc gataatgaac
     3301 ccgcgtttcc cgccgttgat atagatggtt tccaaatcca tcaccgactg ttcgacgtaa
     3361 atatccaatt ttgtccggat gccgaacggc gttgtgccgc cgaccagata gcccgtccac
     3421 ttgtttgcct gtgcgggcgt agcgggttcg atgcgtttcg cacccaaatg ccgagccaga
     3481 ttgcgcgtgg aaatctgttt gtcgccgtgc atcaagacaa tcagcccatt cccgttttca
     3541 tcttgcaaaa caatggtttt aatgaccaag tgttcgtctt tgccgaatag tcgggcaaac
     3601 tgcgccgtgc cgccgtgttc ctcataggca taggtaaaag gttcaaattc gatgccgttt
     3661 tcacgcaaaa cgcgcacggc aggtgttatc ggataagtgt gtttactcat ttttcgtcat
     3721 gcaaaacatg gaaatcggtc aagcgcaatg ttaccatata tcccctttat cgaaggaaac
     3781 cgatatggat atccgatatt tcggcacaac gccccgctat tccgaagccg tcggcgcaaa
     3841 cggcttgatt ttcctctctg gcatggttcc cgaaaacggt gaaacggctg ccgaacagac
     3901 tgccgacgtg cttgcccaaa tcgaccgctg gctggcggaa tgcggctcgg acaaagcgca
     3961 cgttttggat gcggtcatct atctgcgcga tatgggcgac tatgcggaaa tgaacggcgt
     4021 gtgggacgct tgggttgccg ccggcagaac gcccgcccgc gcctgcgtgg aagcgcggct
     4081 cgcccgtccg gagtggcgcg tcgaaatcaa aataaccgcc gtgaagagag acgcggcaac
     4141 tgcataaaca gatttcctta tattttcaga gtaggcaaat gatgaaaacc ttactgccct
     4201 ttttcggcgt ggtgcgcgtc agtggcgaag accgccaaac cttcctgcac ggacagcttt
     4261 ccaacgacat caataatctt cagaccgggc aggcgtgtta tgcgacttac aacacgccca
     4321 aaggccgcgt catcgccaat atgattgtcg tcaaccgagg cggcgatttg ctgctgatta
     4381 tggcgcaaga cctgcttgaa gcaaccgtca aacgcctgcg gatgtttgtg ttgcgtgcca
     4441 aagtcgtttt tgaaatcctt gaagattacg ccgtcggcgc ggaattagaa gcatccgccg
     4501 aaccgcttgc cgcgcaagaa cccagtctcg tctttaccgc cgaatgcgtt tcagacggca
     4561 tctgttccgt cgtcctgccc catcggggca ttttgcacat cgcccccgaa accgccctgc
     4621 cgccttatga tgccgccgcc gaaaacgcgt ggcggctgca cgaaatccgc agcggttacc
     4681 cgtggatatg cgcggctacc aaagaaaccg ccgtcgccca aatgctcaac cagcacatca
     4741 tcggcggcgt acacttcaaa aaaggctgct atccgggaca ggaaatcatt gcccgtgccc
     4801 agtatcgggg gcaggtcaaa cgcggcttgg cggtattgtc gggaaattcg gccgtcgaag
     4861 caggaaccct gctgacggcg gacggcgaag aggccggcat cgtactcgac agcgttcaag
     4921 attcggaaaa ctttaccgcc cttaccgtga ttaaattctc cgccgcacaa aaagaactga
     4981 ccgtcccaaa cggcggtatc ttcaaagcgg ttcatctctt ctttaaaacc gaaaatgcag
     5041 aataagccgc cgatgccgtc tgaagccctt cagacggcat tgccgccccg ccaatcggtt
     5101 acaatgccgc ctttccaatt ttcaaacccg aaccgttatg tccaaaaaaa tcaagcaaga
     5161 actcgaaaac aacaaactca gcaaacgcct gcgccacgcc gtcggcgacg cgattaacga
     5221 tttcaatatg atcgagccgg gcgacaaaat catggtctgc ctctccggcg gaaaagacag
     5281 ctatgccctc ttggacatcc tgcgccggct ccaagccagc gcgccgattg atttccaact
     5341 agttgccgtc aatctcgacc aaaagcagcc gggcttcccc gaagaagtat tgccgaccta
     5401 tctcgaaagc atcggcgttc cctacaaaat cgttgaagaa gacacctact ccaccgtcaa
     5461 acgcgtatta gacgaaggca aaacgacttg ttcgctgtgc agccgcctgc gccgcggcat
     5521 cctctaccgc actgcaaaag aattgggctg caccaaaatc gccttgggac accaccgcga
     5581 cgacatcctc gccaccttgt ttttaaatat gttttacggc ggcaaactca aagccatgcc
     5641 gcccaagctg gtgagcgaca acggcgaaca catcgtcatc cgtccgctgg cgtatgtaaa
     5701 agaaaaagac ttaatcaaat acgccgaatt aaaacaattc cccatcatcc cgtgcaacct
     5761 ctgcggctcg caacccaacc tgcaacgcca agtcatcggc gatatgctgc gcgattggga
     5821 caaacgcttc cccggccgca tcgaatcgat gttctccgcc ctgcaaaacg tcgtcccttc
     5881 ccaccttgcc gataccgaac tcttcgactt tgtcggattg gaacgcggtc aaagcctgaa
     5941 acacggcggc gatttggcgt ttgacagcga aaaaatgccc gaacgttttt cagacggcag
     6001 cgaagaagac gaaagcgaaa tcaaaatcga gccgcaaaaa gccgaacgca aagtcatcaa
     6061 cattctggcg aacaagccga aaacctgcgg cgcgtaaccc cccgccgtca aaaatgccgt
     6121 ctgaagcccg tttggcgttt cagacggcat tttatagtgg attaaattta aattaggaca
     6181 aggcgacgaa gccgcagaca gtacaaatag
//
LOCUS       DAWHXZ010000121         6169 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000121 DAWHXZ010000000
VERSION     DAWHXZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.121"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            238..684
                     /gene="yciA"
                     /locus_tag="WIE35_001431"
     CDS             238..684
                     /gene="yciA"
                     /locus_tag="WIE35_001431"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ4154340.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKIEVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLPTP"
     gene            complement(735..1556)
                     /locus_tag="WIE35_001432"
     CDS             complement(735..1556)
                     /locus_tag="WIE35_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ4154341.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQVLADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGDTEFSNVRFKGDDERAAGVYESVEFIRPEDIAETSLW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1675..2133
                     /locus_tag="WIE35_001433"
     CDS             1675..2133
                     /locus_tag="WIE35_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ4154342.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2297..3412)
                     /gene="lst"
                     /locus_tag="WIE35_001434"
     CDS             complement(2297..3412)
                     /gene="lst"
                     /locus_tag="WIE35_001434"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ4154343.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKAMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPKDYWLKPVYALFTQSGIPILTFDDKD"
     gene            3512..3616
                     /locus_tag="WIE35_001435"
                     /pseudo
     CDS             3512..3616
                     /locus_tag="WIE35_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            3639..3779
                     /locus_tag="WIE35_001436"
     CDS             3639..3779
                     /locus_tag="WIE35_001436"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix 2"
                     /protein_id="HEZ4154344.1"
                     /translation="MPPHIRHSTSDTAAKSGKTISPFLSRRRVQTRQDRTKREQSRQN
                     MP"
     gene            3846..6071
                     /locus_tag="WIE35_001437"
     CDS             3846..6071
                     /locus_tag="WIE35_001437"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ4154345.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     TKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT         1642 a         1806 c         1380 g         1341 t
ORIGIN      
        1 gattcccgcc tgcgcgggaa tgacggttca gttgcttagg gtaggatttg gcgggattgg
       61 tgggctgaag cccaccctac ggccccgccc tacgcatccg ccctacaatg cgccttgcga
      121 atctgcccta cgcccgccct tgtatgtctc aaatgggtta aactacgcac ttcccgactt
      181 cccgtttcag gcaggggtgc aggtccgatt tatatttcaa taaaacaagg aaactttatg
      241 cagcacgaag aaggcaaccg ccaacgccct caaggcgaac tgctcctgcg taccgtcgct
      301 atgccgcgcg ataccaatcc caaccaagac attttcggcg gctggattat gtcgcagatg
      361 gatttgggcg gcggcatatt ggcggcggaa atcgcgcggg gacgcatcgt taccgtcgcc
      421 gttcaggaaa tgaactttat ccgcccggtc aaggtcggca acgtcgtctg ctgctacggg
      481 cattgcgtcc gcgtgggcaa tacttccctc cagcttaaaa tcgaagtctg ggtgaaaact
      541 ttgatgaacg attgcgttac cgaagaccgc tacctcgtaa ccgaggcggt gttcacttat
      601 gttgccatcg atgcggaagg caatccgcgc ccgattccga aagaaggcaa ccccaaattg
      661 tcgggcttat tgcctactcc gtaaaaatac ccgtaaaaat gccgtctgaa actgtcttca
      721 gacggcattc cgcatcattt gaaccaggac ctgatgcggg aaaacagcga catactctgt
      781 ttttcaaagt cttcccgcac ttcgggcacg gcttttttta tcactttcat tcctgcaaaa
      841 gtctgcgcca cgggcataat ttcaatcgtg ttcacattca tatgcgccgg ccggcggtac
      901 agccacaatg aggtttccgc aatatcttcg gggcggataa attccacact ctcatacacg
      961 cccgccgccc tctcgtcatc gcctttgaag cgcacgttgg aaaactccgt atcgccgcac
     1021 aaacccggct cgatattggt aacgcggata tttttatccg ccaactccgc gcgcaaattc
     1081 aggctgaact ggcgcacaaa cgccttggtc gccccgtaaa cgttgctgcc ggcataagca
     1141 taattacccg caatcgaacc caaattcatc acataaccgc cgccgcgttc caccatttgc
     1201 ggcaaaatct tgcgcgtcag gaacgtcaaa cccaaaacat tggtttgaat cattgtttcc
     1261 caatcttcaa aatccgcctt gtccgccgtg tccaaaccca aagccagccc ggcattgttg
     1321 atgaggcagt cgatgtcgga aaattcatcg gggatgccgt ttaaggcatt ttccaccgac
     1381 tcgcgtcgcg acacgtccat ttccaaaggg taaaacaaag cacccaattc atccgccaaa
     1441 acctgaagcc tgtccgcacg gcgcgccgca ccgataacgc ggtatccagc cccgacaaac
     1501 gcgcggcaca tcgcttcgcc gaatcctgcc gaagcaccgg taattaaaac agccattgtt
     1561 tttcctttct ttctcgtttc ccaaacctgt ttcaggtatc ataacaccct tcagacggca
     1621 tcgcgccgac tgaaaatgac aacaaccata ccaccacgac aggacacatt caatatgaaa
     1681 acccaaatca gccttgctgc tgccgccatc acactgctcc tttccgcctg cggaggcagc
     1741 ggaatacctt cccaacccaa aggcgaaatt tccgaaaacc gcaccgccgc gttcaaatct
     1801 atgatgcccg acttctcgcg tatgggcaaa atggtcaaag gcgaagagcc ttacgatgtc
     1861 gaaaaattca aacaggctgc cgcggcgttt gccgaaagca gcaagaaacc gttcacactt
     1921 tttgagtccg atccgcaagg caacggccgc gccctgccag ccgtttggtc ggatggtgca
     1981 aaatttgaag ccgaaaaaac aaaattcgcc gccgccgtcg aaaaactcaa tgccgcagca
     2041 caaaccggca aactggatga aatcaaagcc gcctacggcg aaaccggcgc aagctgcaaa
     2101 tcctgccacg acagtttccg cgcacccgaa taacatccga aacagaacaa tgccgtctga
     2161 agccttcaga cggcattttc tttgccattt cagcacacgc aaagcaatca gaaattatcg
     2221 tcaattcaaa aaatacgcca tatcctgatt tttgccttac ttactccgcc attttctatt
     2281 ttgatttgct atgcgattaa tctttatcgt caaatgtcaa aatcgggatg ccggattggg
     2341 taaacagggc atacaccggc ttgagccaat aatctttcgg aagggaagcc ggtttcaatg
     2401 cgtaaacgtg cacattggga aaatccttca tcgtcaacgc cgcgccgctg aaaaaggtat
     2461 aaatttcata cctcgtatgc gggtttttct taatctcgcg caaaatatag tcttcgatga
     2521 catagggcga atttaatgtg gttacgccgg aaagcccgta ggtttggcgg gggtgcggtg
     2581 cgacatattg tatgttgaag ttttttgccg ccttttccga aatttccttc atctccttgt
     2641 cgggcgaacc caaaagtatc cgcaccgtgc cgcccgtttc atcccccgct ttcagttcgg
     2701 acgcatcgaa cagcggcagg taagtcatct tgcggcggcc gtcgtccata atgtttttca
     2761 aacccttgaa tatcgtgtaa tgctcgtcgg aagcattacg ggttttggcg atactccaat
     2821 ctccgatcat catccgggca aaattccgct tgatcgtccc gtttacagaa aactcatcgc
     2881 ccaaatagct gctgctttga attaaattgc ctgtcccgtc gtcaaaggtt ttgatttccg
     2941 catccgggta agtgctcaaa aaggcggcaa tgctgacttt ttccaaactt gccaaataaa
     3001 tccgcttgac cttcggcagc agcattgcct tcaccttcag ctccgccatc gtcggaatga
     3061 aattaaacga tttgttcaaa ccgtagggca ggtggaaaaa atacgcccgc tccgccttat
     3121 cctttatctg attgaaataa taatcgtatt tttcattcct gttttcagac atcagcacca
     3181 cataaaaccg ctcccccgga tgctgcgcca taatcctttc cgccaccttc atctgcaata
     3241 tggtatagca gaaaatcaga ttgaccggtt ccccctcttc attgaagagt ttgtccttca
     3301 gcagggaaac cgcattcctt tccccctgat ttacccggtc aaatgtataa aatatcccga
     3361 aacaaaaaac aatcagacac aacacggtca aacaagcctt tttcaagccc atatccctaa
     3421 aactccattc cgacaaatta aacatacgcc ccgagtgtcc cgggcggaaa aatgcggaat
     3481 tttagcagat attccgacag ttaccgaata ttatagtggt ttaaatttaa atcaggacaa
     3541 ggcgacgaag ccgcagacag tacaagcagt acgacaaggc gaggcaacgc tgtactggtt
     3601 taaatttaaa ccactataaa atcaatttca ccgaaaatat gccgccgcac atcaggcaca
     3661 gcacctccga caccgccgcc aaaagcggga aaaccatttc ccccttcctt tcacgccgcc
     3721 gcgttcaaac acgccaagat cgaacaaagc gcgaacaaag ccgccaaaat atgccataat
     3781 agccacgcac attccccgcg caatgtgttc caaaagaaaa aaccaaccta caagagaata
     3841 atcctatgac tcaaaaatcc accattgttt atacccatac cgacgaagcc cccgcgctgg
     3901 cgacccaatc gctgctgccg attgtgcagg cttttgcccg ccacgccgat attgatgtca
     3961 aaactgccga catttccctg tccggccgta ttttggcggc attccccgaa tatctgaccg
     4021 aggcgcagcg cgtacccgac gcgcttgccg aattgggcga actggtaaaa caacccgatg
     4081 caaacgtaat caaactgccg aacatcagcg catccgtacc tcaactgacc gccgcgatta
     4141 aagaattgca gtctaaaggc tttgccgttc ctgactatcc tgccgaccct caaaccgatg
     4201 aagaaaaagc cgtacgcgaa cgctacgacc gcatcaaagg cagcgcggta aaccctgtcc
     4261 tgcgtgaagg caactccgac cgtcgcgccc ccaaagcggt gaaaaacttt gccaaaaaaa
     4321 atccgcacag catgggcgca tggacaaaag actccaaaac ccacgttgcc accatgcaaa
     4381 gcggcgactt tttccataac gaacaatccg ttaccgtacc tgaagcgact tccgtatcca
     4441 tcgtgttcac cgacaaacaa ggcaataaaa aagagctgcg cgcgcctgtt gccctgaaag
     4501 ccggcgaaat catcgacgca accgtgatga gcaaaaaagc cctgctcgcc ttccttgccg
     4561 aacaagtgaa agacgcgaag gcaaaaggcg tattgttctc actgcatatg aaagccacga
     4621 tgatgaaagt gtccgacccg attatcttcg gacacgccgt caaagtgttc ttcgcgcctg
     4681 tatttgaaaa attcggcggc aaactggctg ccgcaggtgt caacgtcaac aacggcttcg
     4741 gcaacctgct ggccaatctg gacaaactgg atgcggacac ccgcgccgcc gtcgaagctg
     4801 aaatcgccgc cgtttacgcc gccaaccctg atttggcgat ggttgattcc gataaaggca
     4861 ttaccaacct gcacgttccc agcgatgtca tcgttgatgc ttctatgcct gcgatgattc
     4921 gtaactccgg ccgtatgtgg gataaagacg gcaaagcgca agacaccaaa gccgtcattc
     4981 cggacagcag ctatgccggt gtttaccaag caaccatcga cttctgccgc gaacacggcg
     5041 cattcgatcc gacaacaatg ggtactgtgc ccaatgtcgg tttgatggcg caagcggcgg
     5101 aagaatacgg ctcacacaac aaaacctttg aaatcgaagc cgacggccaa gtccaagtca
     5161 ttgatgcggc aggaaaagtc ctgatgcagc atgacgttga agcaggcgac atttggcgta
     5221 tgtgccaaac caaagacgct ccggttaaag actgggtgca acttgccgtc aaccgcgccc
     5281 gtctgagcaa cacgcctgcc gtgttctggc tcgacgaaaa ccgtccgcac gacaagagcc
     5341 tgctcgccaa ggttaaagcc taccttgccg aactggatac caatggcctc gacatccgcg
     5401 tcctcgctcc tgaagaagcc gccaagttca gcttgggtcg tctgaaaaac ggcgaagata
     5461 ctatctccgt aaccggtaac gtcctgcgcg actacctgac cgacttgttc ccgattttgg
     5521 aactcggcac aagcgcgaaa atgctgtcta tcgttccatt gatgaacggc ggcggtatgt
     5581 ttgaaaccgg cgcgggcggt tctgcaccga aacatgttca acaattcctc gaagaaaacc
     5641 acctgcgttg ggactctttg ggcgaatttc tcgcactcgc cgtatcgttt gaacatctgg
     5701 cgcaaaaaac cggcaacacc aaagcccaag tcctcgccga cactttggat gcagccaccg
     5761 aaaaactgct gttgaacgac aaatcgccta aacgtaaagc cggcgaactc gacaaccgcg
     5821 gcagccattt ctacctcacc ctctactggg cgcaagaatt ggcggcgcaa gacaaagatg
     5881 ccgaactgaa agccgcattt acgccattgg cagccgcttt gaccgccgac gaagcgaaaa
     5941 tcgttgccga gctttccgcc gtacaaggca aagcggccga catcggcggc tactacgccg
     6001 ccaatcctga aaaagccgca caagtgatgc gtccgagcgt aacctttaat caggcactaa
     6061 acgccttata agcacaaagg taaaaatgcc gtctgaacat tcgtcgttca gacggcatta
     6121 gttatcccta tctgcctgat tatgattaaa cgccgcaccc attggatat
//
LOCUS       DAWHXZ010000122         6108 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000122 DAWHXZ010000000
VERSION     DAWHXZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.122"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(209..1201)
                     /gene="pdxA"
                     /locus_tag="WIE35_001438"
     CDS             complement(209..1201)
                     /gene="pdxA"
                     /locus_tag="WIE35_001438"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ4154346.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAPGEGELEVLHIPTAEAVAAGRLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGESGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            1361..2788
                     /locus_tag="WIE35_001439"
     CDS             1361..2788
                     /locus_tag="WIE35_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ4154347.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            3113..5008
                     /gene="mnmG"
                     /locus_tag="WIE35_001440"
     CDS             3113..5008
                     /gene="mnmG"
                     /locus_tag="WIE35_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ4154348.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELNLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPEDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            5076..5660
                     /gene="rnhB"
                     /locus_tag="WIE35_001441"
     CDS             5076..5660
                     /gene="rnhB"
                     /locus_tag="WIE35_001441"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ4154349.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPEIFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVAWHVAAASPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
BASE COUNT         1442 a         1775 c         1609 g         1282 t
ORIGIN      
        1 gattcccgca acctttcgtc attcccgtga aaacgggaat ctagaacctc aaactttcag
       61 ataatctttg aatattgccg tcgcccgaag gtctggattc ccgcctgcgc gggaatgacg
      121 atttggaaat tacccgaaac ccaaaaacaa ctgaaaccga acagaccgga ttcccgcctg
      181 cgcggaaatg acgggtcttt tatcatcttt aaaggctgcc gcgcgccatc tcgacggcgg
      241 tctccacggc agttatcagg ctgccggaat ccgccctgcc ggttgccgcc aaatcgagcg
      301 cggtgccgtg atcgacggag gtgcggataa agggcagccc cagcgtgatg ttcacgccct
      361 gtccgaagct gtggtatttc aacacgggca gcccttggtc gtggtacatc gccaatacgg
      421 catccgcacc ttcgagcata aacggttgga acaaggtatc cgccggatac gggccggcga
      481 ggtttatccc ttcgcggcgc aggttttcca atgcggggat aatggtgtcg gtttcttcgt
      541 gtccgaggtg tccgctctcg ccggcgtggg ggttgagtcc ggcgactagg attttgggat
      601 ttttgatgcc gaatttgtgt tttaagtcgt gatgcaaaat gcgtgcgacg ctttcaatca
      661 gcggttgcgt gatggcggcg gcaacgtctt tcagcggcag gtgggtcgtt acgagggcga
      721 cgcgcaggcc tttgccggca agcatcatca caacctgccc cgtgccgctt ttttccgcta
      781 gatattcggt gtgcccgctg aaaaaacctg tgcttgcgcg cgcgtcgttg atgatgcctt
      841 tgtgcagcgg cgcggtaacg atgccgtcga aaatgccgtc tgaaatgcct gcgagcgcgg
      901 tgtccaaaag ttgcagcaca taggcggcgt tggcgggatt gagccgccct gccgcaaccg
      961 cttcggcggt cggaatgtgc agcacttcca gctcgccttc tccgggtgcg ccgcctgatt
     1021 ccggatcgaa gtcgcgcaag acgacgcttt tgcccaaggc ttcggcgcgc gcgcgcaata
     1081 ggtttttgtc gcccaatacc gcgcagcggc agggcaggcg tgcaaacgcc aagtccaaac
     1141 aaatatcggg gccgatgccg gcaggctcgc cggaagtaac ggcaaaaaca ggctgtttca
     1201 tcgtgtttgc tccaaacaaa atgcgattct aacgccgcag ccgcgcggcg atgtaaattt
     1261 ttctgatttt gttgacaatc tgctagaatg ggcgtttaca aaatttaaac cctgcttgca
     1321 taccgccaat atgtgcgagt ttcaacttta aggaagcgat atgaacgaga actttaccga
     1381 atggctgcac ggctgggtcg gcgccatcaa cgatccgatg tggtcatact tggtttatat
     1441 gcttttgggt acggggcttt tcttcaccgt aaccacgggc tttgtccaat tccgcctgtt
     1501 cgggcgcagc atcaaagaaa tgctcggcgg ccgcaaacag ggggacgacc ctcacggcat
     1561 cacgccgttt caggcatttg taaccggcct tgccagccgc gtgggcgtgg gcaatatcgc
     1621 gggcgtggcc atcgccatca aagtcggcgg accgggcgcg gtgttttgga tgtgggtaac
     1681 cgccttaatc ggtatgagtt cggcgtttgt cgaatcttcg ctggcgcagc tctttaaagt
     1741 ccgcgactac gacaaccacc atttccgggg cggccctgcc tactacatca ctcaagggct
     1801 ggggcagaaa tggctgggcg tgttgttcgc cctgagcctg attttctgtt tcggctttgt
     1861 gtttgaagcg gttcagacca ataccattgc cgataccgtc aaagcggcgt ggggttggga
     1921 gcctcattat gtcggcgtcg ccctggtgat tttaaccgcg ccgattatct tcggcggcat
     1981 caggcgcata tctaaagcgg cggaaatcgt cgtccccctg atggcggttt tgtacctctt
     2041 tatcgcgctt ttcatcattt tgaccaatat tccgatgatt ccggacgtgt tcggtcagat
     2101 tttttcgggc gcgttcaaat tcgacgcggc agcaggcggc ttactcggcg gtctgatttc
     2161 gcaaacgatg atgatgggca tcaaacgcgg cctgtattcc aacgaggcgg gtatgggttc
     2221 cgcgccgaac gccgccgccg ccgccgaagt gaaacaccct gtttcgcaag gtatgattca
     2281 aatgctgggc gtgtttgtcg ataccatcat cgtttgttct tgcaccgcct tcatcatctt
     2341 gatttaccaa cagccttacg gcgatttgag cggtgcggcg ctgacgcagg cggcgattgt
     2401 cagccaagtg gggcaatggg gcgcgggctt cctcgccgtc atcctgttta tgtttgcctt
     2461 ttccaccgtt atcggcaact atgcctatgc cgagtccaac gtccaattca tcaaaagcca
     2521 ttggctgatt accgccgttt tccgtatgct ggttttggcg tgggtctatt tcggcgcggt
     2581 tgccaatgtg cctttggtct gggatatggc ggatatggcg atgggcatta tggcgtggat
     2641 caaccttgtc gccatcctgc tgctctcgcc cttggcgttt atgctgctgc gcgattacac
     2701 cgccaagctg aaaatgggca aagaccccga gttcaaactt tccgaacatc cgggcctgaa
     2761 acgccgtatc aaatccgacg tttggtaaat cccgccctta ccagagccgc ttccccgcga
     2821 agcggctttt ccctttccgc acactgtaaa aacagggcga acaagcgtac aatcccaacc
     2881 ctttactttt gaatccattt cgtttttcag acggcatatt gaatatagtg gtttaacaaa
     2941 aatcgggaca aggcggcgaa gccgcagaca gtacagatag tacagaaccg attcacttgg
     3001 tacttcagca ccttagagaa tcgttctctt tgagctaagg cgaggcaacg ctgtactggt
     3061 ttttgttaat ccactataaa tgccgtctga aacaccgtca ggcaatacac acatgaccca
     3121 catgatttac cccaaaacct acgacgttat cgtcgtcggc ggcggacacg ccggcacaga
     3181 agccgcgctc gccgccgccc gtatgggcgc gcagacgctt ttgctcacac acaatattga
     3241 aacgctcgga caaatgtcgt gcaacccttc catcggcggc atcggcaaag ggcatttggt
     3301 gcgcgaactc gacgcgctcg gcggcgcgat ggcgttggca accgacaaat ccggcatcca
     3361 gttccgccgt ctgaacgcca gcaaaggcgc ggcagtacgg gccacgcgcg cgcaggcgga
     3421 tcgtatccta tataaagccg ccatccgcga aatgttggaa aaccaagaaa acctcgacct
     3481 tttccaacaa gccgtcgaag acgtaacgct cgatggcgac cgcatttcag gcgtaatcac
     3541 cgcgatgggc gtggagttta aagcacgcgc cgtggtgttg accgcaggca cgtttttgtc
     3601 cggcaaaatc cacatcggtt tggaaaacta cgagggcgga cgcgccggcg accccgccgc
     3661 caaatcgctg ggcggacgtt tgcgcgaatt gaacctgccg caaggccgtc tgaaaaccgg
     3721 cacgccgccg cgcatcgacg gacgcacgat tgacttctcc caactgaccg aacagcccgg
     3781 cgacacgccc gttcccgtca tgtccgtacg cggcaacgcc gaaatgcacc cgcgccaagt
     3841 gtcctgctgg attacgcata ccaacacgca aacccacgac atcatccgct caggcttcga
     3901 ccgcagcccg atgtttaccg gcaaaatcga aggcgtgggc ccgcgttatt gtccgtctat
     3961 cgaagacaaa atcaaccgct tcgccgacaa agacagccac cagattttcc tagaacccga
     4021 aggcctgact acgcacgaat actaccccaa cggtatctcc accagcctgc cgttcgacat
     4081 ccaaatcgcg ctcgtccgca gtatgaaagg tttggaaaac gcccatatcc tgcgccccgg
     4141 ctacgccatc gaatacgact acttcgatcc gcgcaacctc aaagcaagcc tcgaaaccaa
     4201 aaccattgcc ggattgtttt tcgccgggca aatcaacggt acgacaggtt atgaagaagc
     4261 cgccgcgcaa ggactgctgg caggcgcgaa cgccgtgcaa tacgtccgcg aacaagaccc
     4321 gctcctgctg cgccgcgaac aagcctatct cggcgtattg gtggacgacc tcatcaccaa
     4381 aggcgtgaac gaaccctacc gaatgttcac cagccgtgcc gaataccgcc tgcaactcag
     4441 ggaagacaac gccgatatgc gcctgaccga agacggctac aaaatcggct tggtgggtga
     4501 agcgcaatgg cgcatgttca acgaaaaacg cgaagccatc gaacgcgaaa tccaacgttt
     4561 gaaaacaacg tggtacacgc cgcaaaaact cgccgaagac gaacaaatcc gcgtattcgg
     4621 gcaaaagctc agccgcgaag ccaacctgca cgacctcctg cgccgcccga acctcgacta
     4681 cgccgcgctg atgacgctcg aaggcgcgat gccgtctgaa cgcctctccg ccgaagtggt
     4741 cgaacaagtc gaaatccaag tcaaatacca aggctatatc gaccgccaaa acgaagaaat
     4801 cgacagccgc cgcgacatcg aaaccttaaa gctgcccgag gatatcgatt acagcaaagt
     4861 taaaggtttg tcggcagaag tgcagcaaaa gctcaaccag cacaaacccg aaaccgtcgg
     4921 gcaggcaagc cgcatttccg gcgtaacccc tgcggcagtg gcattgctga tggtgcattt
     4981 gaagcgcggg tttaaagacg cgaaataaac acatcggcgc gatgccgtct gaaacaccgt
     5041 tcagacggca tcgcgccatc ccgacaggaa acatcatgca catactgacc gccggcgtgg
     5101 acgaggcagg gcgcgggcct ttagtcggca gcgtgtttgc cgccgccgtc atccttccgg
     5161 aaatattcga cctgcccgga ctgaccgact ccaaaaaact cagcgagaaa aaacgcgacg
     5221 cgcttgccga aatgataaaa aatcaggcgg ttgcgtggca cgttgccgcc gcctcgcccg
     5281 aagaaatcgc cagcctcaac atcctgcacg ccaccatgct cgcgatgaaa cgcgccgttg
     5341 acggtttggc tgtgcgtccc gaaagaatat tcatcgacgg caaccgcatt cccgaacatt
     5401 tgaacatccc tgccgaagcc gtcgtcaaag gcgacagcaa aatcatcgaa atctccgccg
     5461 catccgtttt ggcaaagacc gcacgcgatg cagaaatgta cgcactggcg caacgccatc
     5521 cccaatacgg tttcgacaaa cacaaaggtt acggcacgaa gcagcatctg gaagccctca
     5581 aacaatacgg cgtgctgccc gaacaccgcc gagacttcgc ccccgtcaga aacctgctcg
     5641 cgcagcaggc cttgttttaa accggcacaa aaatgccgtc tgaacttcag acggcatttt
     5701 cagcatcgca acttcaaaga gaaagaaccc aaaccgtcat tcccgcgaaa aatagaaaat
     5761 caaaaaaaaa cctaaaatcc gtcattcccg cgcaggcggg aatccaatcc gtccggtttc
     5821 cgtttttttt gaatttcagg taacttccaa accgccattc ccgcaaaagc gggaatctag
     5881 aaactcaaag ctgcaagaat ttatcaaaaa tgactgaaac tcaaaaaacc ggattcccgc
     5941 gaaaacagga atccggagtc tcagggttgg aaaaaccgtt tttcccgata agtttccgta
     6001 ccgacagacc tagattcccg cctgcacggg aatgacgata tttcagtttt gtgtttttga
     6061 tttttttgtt tttgcgggaa tgacgatata gtggattaac aaaaatca
//
LOCUS       DAWHXZ010000123         6107 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000123 DAWHXZ010000000
VERSION     DAWHXZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.123"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(169..2256)
                     /locus_tag="WIE35_001442"
     CDS             complement(169..2256)
                     /locus_tag="WIE35_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ4154350.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(2259..3524)
                     /locus_tag="WIE35_001443"
     CDS             complement(2259..3524)
                     /locus_tag="WIE35_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ4154351.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKLEQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(3491..3705)
                     /locus_tag="WIE35_001444"
                     /pseudo
     CDS             complement(3491..3705)
                     /locus_tag="WIE35_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4745..4981)
                     /locus_tag="WIE35_001445"
     CDS             complement(4745..4981)
                     /locus_tag="WIE35_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ4154352.1"
                     /translation="MKELHTSELVEVSGGKFHIFAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGISKKW"
BASE COUNT         1941 a         1084 c         1039 g         2043 t
ORIGIN      
        1 aaaaaacaac aaattcaaca gaaaaaggaa actgaaaaat cgccaaaaca caaagccagt
       61 cgtgatgatt gggaaatgga acgttaaacc gctattttca tgtattctat taaattattt
      121 gaaaaaagac ttcacttttt gttaaagaaa aatgaagtct tgttctgttc aacctaaatt
      181 cagaatttta tctgccgatt gaattgtttc ttggcggtgt gcaaccatta ttttcataat
      241 accaagactt tttaggttat ggttaatttt ttgttcattt tctacatcta aatggctact
      301 tgcttcgtct aaaaatagaa ttttgggtcg tttatacaat gcacgagcca agataactct
      361 ctgcttttgt ccacctgata agatatttcc catatcgcca atcaaggtct catagcccat
      421 tggcatttta agtatatcgt catgtatttg tgccattttt gcacattgtt caatgagctc
      481 catatttggg ctttcatcaa aaaatgaaat attttcccca atagaacctg caaaaaggac
      541 atcatcttgg cgaacaatcc cgctcaatcc cctaataaag gatggagaaa cttgatatat
      601 atcatgccca ttaatactaa ctgtaccagt ttcaggtttt aggctacctg ttaaaatgtt
      661 taacaaagtg gacttccccc gaccagattg tcctgttaaa acaactgctt cattatcttt
      721 aaattccaaa ttaatgtttt caaaaagata tggctcatta tcagcatatc tgaatgagac
      781 gttttcaact ttaagaacca gttgttcatt atctaattta ggtatatgat tatacttaat
      841 aatttcagtt tctgtttcat ttaaagtaat gtcagccaaa cgttcagcat gaagccctaa
      901 cattttgatt tggatgtatt ggtcaacgag agaagctgtt ctgctttcaa attgcccttt
      961 ataagccaaa aaagccatca gaacaccgac tgtaaatgaa ccatctaaaa ttgcgcttgc
     1021 accaagataa attatgataa tattttccat gctaaacaac agtttatttg aaaattcaaa
     1081 taaagcagag agtttatctg ttgtcagctt ggtattgact gtattcacaa ataggctcat
     1141 ccaagtgcca tgtctttgat aatgtttatc aaataattta actgattgga taccacgaat
     1201 ggtttccatg aaatatgagt tttgtttggc ttcatgaaca atattttctt ctgttgcatt
     1261 tcttaatggg taatatgcaa gccaacgaat tagtatgtac aaaacaagtg ttaaaagaac
     1321 aatcagcgat aattgagtgc tgtaaattgt cattaacacg aaagtaaaaa cagccattaa
     1381 gctatttaaa actaaaacaa aaaaagtaga agttagtgtt tcttggatat gatctattga
     1441 accaaatctt gaaatcacat ctcctaaatg tcgtttactg aaatagtcat taggtaagtc
     1501 aagtaacctt ttaaatatat tggctttcca ttgtaaatta agagttgtag ataggtgcat
     1561 acctacccat gcttgtaaca ggctaattaa ctgttgcagg atagccagta aaccaaatcc
     1621 caaagtaagg gtcagtaata aatttttatc agcagttaca atgacatggt ctattaccca
     1681 ttgcataaag aatggactaa ccaatgcaaa gacttccaaa gaaatagcta atataagcat
     1741 ttgaattaaa gagcgtttta agcctgaaat cccccttaat agagataata ttttgatttt
     1801 ctttgtttct tttttctctt caaaatgggt attggggaat aattctaggg caatccctgt
     1861 gaatttttgt gaaacttcgt ccattttgat ttttcgcata ccgacagcag ggtccataat
     1921 gacgatactg tctttggaaa tggaacaaag tacaacaaaa tggtttaagt tccaatggag
     1981 aatgcagggt agttgtaaat ttgacagctc atctaactct aaacgcaaag ctcgtggcgt
     2041 taaattcatt tcattgccaa atctcatgat gtctgcaaga tttgcgccct ttaatgacag
     2101 ggtgtatttt tggcgtaacg ttcttaaatc agtgtgaaag ccataatagg acaagatgga
     2161 tgtcaggcat gctaaaccac attcagcaac ttctgtttgc agaatgacag gtagcttttt
     2221 gttaaatcca aaggacagtc ttgataaata atccattttt aattgatttt tcctgaaatg
     2281 ctgtaaagtg ggtcaagtac ccattcgtac aatttttttc gttcatggag aatatctgct
     2341 tctaaaatca tgccaatttg aagcggcttg ttttctccgt atgctttaat cgtttgtttt
     2401 tccaatttaa ctttcacaag ataggcaggt tcatttaata aggttgggtt agtgaaaatg
     2461 atacctaaac ctgatagctc ttgtttaccg agagcagttc tggcaactga aataatttct
     2521 cctgtggcat gtccaaattt ttggtaaggg tacgcttggt aacgtaaaac aactttatct
     2581 ttcggtttaa taaaaccaac agctttactg ggtatgtaaa gattggcgac caattcagtt
     2641 tgttcaggga caatgcttaa cagcaattta gacggttcaa cttgttgccc tatatcaaca
     2701 ttaattgttg atatataacc tgatttacta gctcgtatgg tttgttcgga tttcaaatca
     2761 aaatccaaaa tttcttggtt catttccgta atcgcacggt tgagttggct caattcggtt
     2821 ttatggcgtt caggcaggct gcttaatgta attttctgtt catcaagttc cctgattgca
     2881 ttattttgtt caagttttag gctctccaaa cgtgagcgtt gttccaataa atggctttcg
     2941 gcggtcatct tatcttgttg ggatactgcg ccttgactgg ctaaaaactt gttcttgtta
     3001 agggtttttt ccgctaaacg aatttgacga ttttgccctg taatttgctg tttaatattc
     3061 tctaattgat tgtttaaacg atgaatgttg ttatgaacat ttttttgctc attttgatga
     3121 atgcgcttta aacgttccaa ttcttgtaat gccaaagttt ttttaaggtt ggcttctgct
     3181 gccaatttgg cttgtacgtt tcctttttcg ccaaaacgcg atgtggaaag tttgaacaat
     3241 ggttcgccag ctttgacaaa gttaccatct tcaacaaatt tatgcgtaat cgtgccgata
     3301 tcggaagagt aaacacgaac cacccccata gttggaagta attgaccttc aacggttgtt
     3361 ttattggtat agctaccaaa aatcaaaaag ataatgatac acagagcaat gagaaaagcg
     3421 caaaaagtca gaaataaaaa agagaatgga cgggtcaaga taacctgacc tgtccactta
     3481 ttttgttggg ctacaaagac ttcttttcgg aataagtggg acatttatta ttatttctta
     3541 aaaaaatgga tagaaaaaca ccaataatta aatacttatg cctaataaca taaatttgct
     3601 attgattgat gtttctattt taattacatt aaatttaacc aataatccag aaatggttgc
     3661 agctaaaatg caaaagacag tggcgtaaat ggtttcagta ttcataattt ttctctttca
     3721 aaattgttca atctaaatag aaaaaggtaa cttcaataca tattgctgta actgaaatta
     3781 ccttttcctt aatgatgtaa ttatcttatg gcttcatcat gccacgaccg atattgtatc
     3841 cgccagcagc agagaaaata ccagctccaa ttgcgccaac ccagcctact ggtcctgtcc
     3901 acttgttccg ttgagctaca aagacttctg gtctgaaaaa tgagtgattg ttagacatgt
     3961 gtacttattt atgtttcttt gattgggata aatttgtaaa aacaaaaaaa aggcaagttg
     4021 ttattataga agaacttaaa atgcttaaca tactcattga ggtttgtacc acataatgag
     4081 atacgagata atttgttggg agtaaaatca aaaaaagatt aaatatttaa aataatcact
     4141 atattttttc attatttgtt cctttttgta tttaagttct tatgaaaaac tagggtattt
     4201 tgaatagaaa atatcaaaat accctagttt tattaaggaa tattaccacc aacaagtgct
     4261 tccatattgt acagcaccag tgattgcacc accaatagct ccgaatttcg cacctggaat
     4321 agcaccaata ccacctgcaa atgaaccaac aattgcccca ccagctgctc cacctaatgc
     4381 actaccaatg gtattttttg agaaatcacg ccagttacaa gcagcaccag aaacttattg
     4441 aatttcattc aaagtcaaaa cttgcataat attcaccttt gttaagtgtg gttgagaaaa
     4501 aagtaaattt tcatcgttac tcattatttg caatcatgca tatactgtaa ccaagcacta
     4561 tattcagttg gatcatagtt tttcctactg ggttgtctga tatgactatt ggcactattg
     4621 gcacaattgg gattagcaac tttattgtga gtacctaatg gattgatatt tcctgtaaag
     4681 tggctattgc atgcgcttaa tgagcataga tttattaatg tcagaaagga taaaacaaaa
     4741 aatcttacca ttttttacta ataccaaaag taggtttcga aaactgtaca ccaagacctt
     4801 gtacatatcc aaattgcttg ctaacagaaa actctactcc actattgtta ggggaatagg
     4861 aagcaccaat tttaccaaca gcaaccatat cttttttacc taggttgccg ccaccctgtg
     4921 caaagatatg gaatttgcca cctgacactt caactaattc agaggtgtgt aactctttca
     4981 tgattttctc ctgtaaagaa gtatggttgg aaatgtaaat cagaattaac ctttcttaag
     5041 tttaatttaa tacttttttt gcaagtattt tattttaaat taaatcaata ttttaaaaat
     5101 atttacttgt atactgaata tttacttgta tactgaaaaa gaaactccta atggtggagt
     5161 ggggcttttg tttaagtaag cattgaaaaa atttaaattt cccattatga ttaacttaat
     5221 gcaagagctt tttcaggacg tacaaaccag cgaacgggcg ttctgatgtg ggcaggttgg
     5281 ttgtcgctaa ccaatttcag tagctcaatc gtcagattgg tatccggccc tatcaagcta
     5341 aggagaggct gcggctacta cacaaagcac ctgaaattat tgggtgcttt tgtcttttta
     5401 tggcattgta ccaatttgtg taccacgagg gcttgttcgc ccaaacgcgg taacaactga
     5461 atacacactt tccccctcaa cttctttgcg ttctaaaacg actgtcccaa tccgagaata
     5521 gacaatcata ggacgatgat tgccccttac atcactaaat tcactaaaaa tttttgcgcc
     5581 tgattgaaaa atcatttcca cataaaaggg ttacacctgt ttcatcgtgg cttaatccaa
     5641 accgcgttaa atctgctttg tgttctgcca aaatgtgaat cacaccaaag ccgtgattaa
     5701 cctttttgtg ttcgccgtgt tttaaaacaa tttgacctgc cgagaagtct gctctattgt
     5761 tcggacaata gggggaatat accaattact ttaactacat cattttttga catttttctt
     5821 gcctttcaat ttaaaatcca aaatcatact gccataattt agcaatccaa aaaaatttag
     5881 gcagcagtca tggtttcgta ttttagaaaa cgaaacttgg tttggtgttt tgaagcattt
     5941 cagtacacaa acatggaatt ttaaattgtc ttgtgttgcc ttgatttagg atgttccagt
     6001 tttttaataa atagtttggg gctgtcctag ataactagga caaacttgat tttactaatt
     6061 gttttaaaat ggaacaagaa cttttatctc actgttgtta aaacgtc
//
LOCUS       DAWHXZ010000124         6099 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000124 DAWHXZ010000000
VERSION     DAWHXZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.124"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..954)
                     /locus_tag="WIE35_001446"
     CDS             complement(<1..954)
                     /locus_tag="WIE35_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HEZ4154353.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALIREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERV"
     gene            1215..1311
                     /gene="ffs"
                     /locus_tag="WIE35_001447"
     ncRNA           1215..1311
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WIE35_001447"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1479..3170
                     /gene="dld"
                     /locus_tag="WIE35_001448"
     CDS             1479..3170
                     /gene="dld"
                     /locus_tag="WIE35_001448"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ4154354.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            3302..3409
                     /locus_tag="WIE35_001449"
                     /pseudo
     CDS             3302..3409
                     /locus_tag="WIE35_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            3465..3836
                     /locus_tag="WIE35_001450"
     CDS             3465..3836
                     /locus_tag="WIE35_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ4154355.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            3991..4539
                     /locus_tag="WIE35_001451"
     CDS             3991..4539
                     /locus_tag="WIE35_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ4154356.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            4673..5764
                     /locus_tag="WIE35_001452"
     CDS             4673..5764
                     /locus_tag="WIE35_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ4154357.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLTLAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
BASE COUNT         1577 a         1844 c         1515 g         1163 t
ORIGIN      
        1 cacgcgctcg atgcgcagcc attcgccctg cgggttgacc atctgccagt aggcgaggtt
       61 gtccaacgcc gtcccccaca gcacggcttt tttaccgccc gcgttcatcg ccacattacc
      121 gcccacacag gacgcatcgg cggaagtcgg atcgacggcg aacaccaaac ctgcctgatg
      181 cgcggtttct tccacccgcc gcgtaaccac gcccgcgccg caccggataa tcggatgctt
      241 gccgtccaag cctgccagct caacgtattc aacaccacga tgcttgtcga gtttttcggt
      301 attgatgact gcgctgtttg cgtccaaagg aatcgcgccg ccggtataac ccgtgccgcc
      361 gccgcgcgga ataatgacca aatccagctc gattaaggcg cgcaccaaag gcgcgatttc
      421 cgcctccgtg tcgggattga cgacgacaaa cggatactcc acgcgccagt cggttgcatc
      481 ggtaacgtgc gttacccgcg ccaatccgtc aaacataata ttgtgcggct tggtgatttt
      541 gctcaaacgc tccaaaatct gccgccgctt ttggctggtt tcatcaaaac tgctatcaaa
      601 acgctcgact gctttttctg ctgcggcaac caagacatcc acttgccgat tatcgtcgcg
      661 gcgtttgcgg atttcgttca ggcggtggcg catttcgcgt atcaacgcgg cgcggcgttt
      721 cggatgctcc agcaaatcat cgaccagata cggattgcgc acgaccaccc aaatatcgcc
      781 caacacttca aacagcatcc gcgccgaacg tccagtttta cgctgtccgc gcaagtcttg
      841 cagaatctgc cacgcctcgt cgcccaataa tcggatgacg atttcgcggt cggtgtagga
      901 agtatagttg taggggattt cccgaatacg ctgaggggcg gtagtcgtgg tcatggtgtg
      961 tccgtgtttg gcagttttta tttgaatgcc gtctgaaaag tcggtttcag gcgagtttga
     1021 aaaggcgata atgtagttca atttttgata cttttcaatg ccgaaacagg aaataccctt
     1081 atagaaacaa aataacgctt tgagaaattg ttgacaatat tatcaaatat aaattttagg
     1141 tggtgtttcc ggcgtatgac cgtatcaaaa cggttgagtt tttcagaaaa cccgttataa
     1201 tgccgtttct ctgaggcggg tctccccgca tggcaaatcg gaacaccggg tcaggggcgg
     1261 aagccagcag cccactccga tgcgccagtg ccgggggttt ggcccgccgc cctatttgaa
     1321 acgccggagc ttcgatgttc cggcgttccc gtatcgcacc gatgccgtct gaaagccgtt
     1381 cagacggcat ttcctttact ttgccataac gcataactac aatgggaaaa caatttcaat
     1441 gtgcatatgc acctgccgac aattttaagg aaactgccat gagcgcatca caattactgt
     1501 caagactgac ccaaaccgta ggcgaaaaat acatcatcac cgaccccgcg aaaaccgaac
     1561 aataccgcca aggctaccgc ttcggcgagg gtaaggcgtt ggcggtggtt cgccccggaa
     1621 gcattctgga aatgtggaaa attctgcagg cctgcgtcga agcggacgtg attgtgatta
     1681 cgcaggcggc gaataccggt ttgacgggcg gctcgacccc cgacggcaac gattacgacc
     1741 gcgacatcgt gattgtgaac accatgcgga tgaacatcat ccaaaccatc aacaacaacg
     1801 aacaagtcgt ctgcctgccc ggttcgaccc tgaaccagct tgaattgctg ctgaaacctt
     1861 tggggcgcga accgcattcg gtcatcggct catcctgtat cggtgcgtcc gtcttgggcg
     1921 gcgtgtgcaa caactccggc ggcgcgttgg tacagcgcgg cccggcctac accgaaatgg
     1981 cgttgttcgc ccaaatcaac gaagaaggca ggttggaact ggtcaaccac ttgggcatag
     2041 acttgggcga cacgcccgaa gaaatcctga ccaacctgca aggtcatcat tatcaaaaca
     2101 aagacatcaa acaagacgcg ggcaaagggc acgaccacgc ctattgcgaa cacgtccgcc
     2161 aagtggacga accgaccgcc gcgcgtttca atgccgaccc cgcccgccat tacgaagcct
     2221 cgggctgcgc gggcaaactg atggtttttg ccgtccgttt ggacaccttc ccgcaagaaa
     2281 aacaaaccgc cgtgttctac atcggcacga acgacatcaa cgagctgacc gacatccgcc
     2341 gcgccgcctt gggcgaattt gaaagcctgc ctgtttccgg cgaatacatc caccgccacg
     2401 ctttcgacat tgccgacgtg tacggcaaag acacgtttta cgtcatcaaa aaattcggta
     2461 cgcaccaact gccgaaatta tttgacttga aggcgcgcgt ggacaggttc ggcaaaaaag
     2521 ccagcttcct gcccaaacat ttttccgaca aggccatgca gttcgtcagc aaatacctgc
     2581 ccgaccacct gcccaaatcc atgcgcgatt accgcgacaa atacgaacac cacctgattc
     2641 tgaaaatggg cgggaaaggc gtggatgagg cgcgcgcgtt cttaaaagaa tattttgcac
     2701 accacagcgg cgcgtttttc gagtgcaacg ccgaagaaac ccaagccgcg atgctgcacc
     2761 gtttcgccgt cgcctccgcc gccatccgct accgcgccgt gcatgatgac gaagtggaag
     2821 acttggtcgc gcttgacatc gccctgcgcc gcgacgaccg cgactggttt gaaaaactgc
     2881 cgcccgaaat cgacaataaa atcatccaca aactctacta cggacatttc atgtgccacg
     2941 ttttccatca ggattacatc atcaaaaaag gcaacgactg catggcgttg gaacacgaaa
     3001 tgctgcatct cttagaccaa cgcggcgcgc aatatcccgc cgaacacaac gtcggtcatt
     3061 tgtatgaagc caagcccgcg ctcaaacagt tttaccgcaa actcgacccg accaacagct
     3121 tcaacccggg tatcggcaaa accagcaaaa agaaaaactg ggcggaataa gcgcgtccgc
     3181 tttgaaggca ggcaatgccg tctgaaggca aaacgccgtt cagacggcat tgtcgggttt
     3241 gaagttcccc gtcgggtaat cgctttcaaa ccgtatgccg acacacgggc gcgcccctcc
     3301 caaatatagt ggattaaatt taaatcagga caaggcaacg aagccgcaga cagtacagat
     3361 agtacggcaa ggcgaggtaa cgccgtactg gtttaaattt aatccactat aaagtctgat
     3421 cctaccttcc caaagggcag ggtttcaacc gaaaaggaaa cacgatgaaa ccatacaaaa
     3481 tctacaccca tcccgccctg ccgccccaag ccgtcaaaca aggctggtca tggccggggc
     3541 tcttgttcgg cacgctgtgg gcgtgtttca aaagaatgtg gggtttgggg ctggggctga
     3601 cgggcgcgat attcgtgctg gctgtgtttg cccaactggt ttacggcgac acgcccgcca
     3661 cagactcggc gtttaacgta ttgggcttgg cggtttccgt ctggttcggc gcaaagggca
     3721 acagccttta tgcacgccac cttttatcgc gcggctatac cgaattgccc gaaacggttg
     3781 aggcagccaa tccccaagcc gcattggcgc aatatttcgg gcgcgggggc cgataagcgc
     3841 gttttctttt ccatcatgcc gtctgaaaaa tgttcagacg gcatttctta taaccttttg
     3901 tttgcagcac ataaccaatc agtctttcct attccgttcc gaaatgcata ccatgcgccc
     3961 gtacccaata tttgcgaaca aggaaagaaa atggcacgtt taaccgtaca caccctcgaa
     4021 accgcccccg aagccgccaa agcgcgcgtc gaggcggtac ttcaaaacaa cggctttatc
     4081 cccaacctta tcggcgtatt atcaaacgcc cccgaagcct tggcgtttta ccaagaagtc
     4141 ggcaagctca acgccgccaa cagcctgacc gccggcgaag tcgaagtcat ccagatcatc
     4201 gccgcccgta ccaaccaatg cggtttttgc gtggcagggc acaccaaact cgcaaccctg
     4261 aaaaaactcc tgtccgaaca atccgtcaaa gccgcgcgcg ctttggcagc aggcgaattt
     4321 gacgatgcca aactcggcgc gctcgccgcc tttacccaag ccgtaatggc gaaaaaaggc
     4381 gcggtatccg acgaggaact caaagcattt ttcgatgcgg gctacaacca gcagcaggca
     4441 gtcgaagtcg tgatgggcgt agccttggca accctgtgca actacgtcaa caacctcgga
     4501 caaaccgaaa tcaaccccga attgcaggct tacgcctgat acggcaaacc gcccgaatat
     4561 cgggcggttt ttcaaaaaca ccccctcaaa caaaaacaag ccgccaagcg gcagggaagc
     4621 agccgcgcat caggcgcgcc cccgattccc ccgcaaacct taaggaacaa agatgaacgc
     4681 ccaaaccctg attgccaacg ttgccgaatt cgtcaaaacc aagctcaaac ccatagtgga
     4741 cgacatcgac cgcaaaggat actaccccga agcatttatg cgcgaactcg gcgcaatcgg
     4801 tggattcggc gcagtcggta cagaagccga aggcggcaac ggcttgggtt tggcaacgca
     4861 aatcgccgta ttgcgcgaaa tcggcaaaga atgcggcgca acctctttca gtgcgtggtg
     4921 tcaggcggct tgcgcgtggt atctgcacca aacgcccaac cgggccgtca aagacaaata
     4981 ccttgccgac atcctgcaag gcaaagtatt ggcgggtacg ggcatgtcca ataccgtcaa
     5041 acaccttgcc gacatcgaaa aacacaacct ccaagccgag cgcgtggagg gcggctacac
     5101 agtcaacggc gcgctgccgt gggtgtccaa catcggcgaa gaccacattt gggcgaatac
     5161 cgcccaaatc ggcggcggct acgtcatgtt catcacaggc ggacaatggg aaggcgtaag
     5221 cctgcaaaac tgccccgaat tttgcgccct cgaaggcacg cgcaccttca gcctgaactt
     5281 caaagacgta tttatccccg acgaagacat catcgccgcg cccgaacagt ttgccgacta
     5341 catccaaagc atcaaagcag gctttatcct cctgcaaatc ggcatcggcg cgggcgtgat
     5401 agacggcagc ctcggcatca tccgcctcgc caacgtcgtc aacgccgaag tcaaccgcta
     5461 tctcgacgac ggctacgaca gcctcaaaac aaggctggac ggcgcgtggg cagaaaccga
     5521 acggctcgcc ggattggctt ggagcggcac gcccgacaac cttaccaccc tcaagctgcg
     5581 cgaagccgcc gccgtactga cacttgccgc cgcccaatcc gccgccctgc attccggcgc
     5641 aaaaggctac ctgatgcgga gtcccgccca aaggcgcgtc cgcgaagcaa tgtttgtcgc
     5701 catcgtaact cccgcgatca aacacctccg caaagaaata gcggcaatcg aagccgcaaa
     5761 ataaacaggc ggaaaaaacc gaaaccccca tcgcgtcatt cccgcgaaag cgggaatcca
     5821 gcccccaacg taacaggaat ccatcggaaa aaacagaaac cctcaaaccg tcattcccgc
     5881 aaaagcggga atccagcccc ccctaacgca acaggaattc atcgaaaaaa ccgaaacctc
     5941 caaaccgtca ttcccacgga agtgggaatc cagtaaccga aaaaccacag gaatctatcg
     6001 aaaaaaaacc caacccccaa aaaaaccggg cggatacctc accatcctcc caaaccctga
     6061 tttatagcgg atgaacaaaa atcagggcaa gacggcgaa
//
LOCUS       DAWHXZ010000125         6088 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000125 DAWHXZ010000000
VERSION     DAWHXZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.125"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            57..1610
                     /locus_tag="WIE35_001453"
     CDS             57..1610
                     /locus_tag="WIE35_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ4154358.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAISAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASVGLSKELAGLKAQTPIEKIIWTGKSRPVSETAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQTLLKRATVFLGIPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(1701..3728)
                     /locus_tag="WIE35_001454"
     CDS             complement(1701..3728)
                     /locus_tag="WIE35_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ4154359.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPNQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTVIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            4017..6023
                     /locus_tag="WIE35_001455"
     CDS             4017..6023
                     /locus_tag="WIE35_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ4154360.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
BASE COUNT         1553 a         1856 c         1420 g         1259 t
ORIGIN      
        1 cggcatattg ttttcaaatg agggcgttcg ccgtccgcaa ccataaagga agtttcatga
       61 accggactta tgccaatttc tacgaaatgc tcgccgccgc ctgccgcaaa aacggcaacg
      121 gcacggcagt gttcgacggc aaggaaaaaa ccgcctaccg cgcgctcaag caggaggccg
      181 aagccgttgc ggcgtatctg caaaatatcg gcgtgaagtt cggcgacacg gtcgcgctgg
      241 cggtttccaa ttccacagaa tttattaccg cctatttcgc catctccgcc atcggcgcgg
      301 tcgccgtacc gatgaacaca tttttgaaaa acagcgaata cgcgtatatc ctgaacgact
      361 gcaaggcgcg cttcctgttc gcctcggtcg gcctgtcaaa agaattggcg ggcttgaagg
      421 cgcaaacgcc catcgaaaaa atcatttgga cgggcaaaag ccggccggtc agcgaaacgg
      481 cggaaggcga tgcctttttt gaagacgtgc gccgcttccc cgaaaaaccc gacttgggcc
      541 gacaaccccg gataaatgat ttggcacaca tcatctacac ctccggcacg acggggcatc
      601 ccaaaggcgc gctaatcagt tacgccaacc tgttcgccaa cctgaacggc atcgaacgca
      661 tctttaaaat ttccaaacgc gaccgcttta tcgttttcct gccgatgttc cacagcttca
      721 cgctgacggc tatggtgctg ctgccgattt atatggcgtg ttcgattatt ttggtcaaat
      781 ccgttttccc gttttccaat gttttgaaac agaccctgct caaacgcgcg accgtgtttt
      841 tgggcatacc cgcgatttac accgcgatga gcaaggcgaa aatcccttgg tatttcagat
      901 ggttcaaccg cattcgcctg tttatcagcg gcggcgcgcc tttggcggaa caaaccatcc
      961 tcgatttcaa agccaagttc ccccgcgcca aattgctgga aggctacgga ctgagcgaag
     1021 cctctcccgt cgtcgccgtc aatacgcccg agaggcaaaa agcccgcagc gtcggcatcc
     1081 ccctgcccgg tttggaagcc aaagccgtcg atgaagaatt ggtcgaagtg ccgcgcggcg
     1141 aagtgggcga actgattgtc aggggcggtt cggtgatgcg gggctacctc aatatgcctg
     1201 ccgccaccga tgaaaccatc gtcaacggct ggttgaaaac gggcgatttc gttaccatag
     1261 acgaagacgg cttcatcttt atcgtcgacc gcaaaaaaga tttgattatt tccaaaggtc
     1321 aaaatgtcta tccgcgcgag atcgaagagg aaatctacaa actcgatgcc gtcgaagccg
     1381 ccgccgtcat cggcgtgaaa gaccgttatg ccgacgagga aatcgtcgcc ttcgtccaat
     1441 tgaaggaagg tatggatttg ggcgagaacg aaatccgccg ccacctgcgt accgtgctgg
     1501 caaatttcaa aatccccaaa caaatccact ttaaagacgg gctgccgcgc aacgctacgg
     1561 gcaaggtatt gaaacgggtg ttgaaggagc agtttgacgg aaacaaatga acgccgtgcc
     1621 gtccgaagcc ccgtccggca aaaaaaatgc ggtgaatctg cttcaccgca tttttttggc
     1681 aaacgccgga gcgaggatat ttactctgcc aattccacct gctcgtgcgc catcagttcc
     1741 tgaccgacat catccaacaa catcaaataa cgttcgtagt ttttcaaaat gtcggcaatc
     1801 agctcgtcct tgttcatata ctgcacatcg tacccgacgc gcccgtcgaa aaaataagcg
     1861 tagggtttgt aagttgtctg atgccggata tgcggcagct tgccgtcgtt aatcaactgg
     1921 tcggatacat cctgcccgac agacttaatc ccgtacataa aatcgcgcat cgtctctttc
     1981 cgaatgacga actcgattgc gggctcgtcc tgatgaaaca tcttatcgac ccggacgctc
     2041 aagccgtatt cttccgaaag ctcccgttgc aactcgtgca tagcgggcga tgcggtatgt
     2101 ttgaggaatt ttaaaatatc ctgctcctgc gtctggctca ttatctgcac cagccgttct
     2161 ttccacttgc cgcccgtcca aaatacactg gtagggttaa cccgggtctc aaaatatttc
     2221 ttatccgcac tcaatccttt ccacaggctg aaacacatta tcagcatcag cagggcaaac
     2281 ggcagggaaa caatcagggt catagactgc aggttgccga gtccgcccga gcgcatcagc
     2341 aaaacggcaa cggcagacat cagcacgccc cacataaccg cctgccaccg tggcgcgctc
     2401 aagcctttgt cccgagaggt aatattgttc aggacataaa tcccggaatc ggcagaagtt
     2461 acaaaaaaca gagaaatgac cagcaggctg acgatgctcg tcaattcggg cagggggagg
     2521 taattaaaga atttaaaaag cagcgtttcc ggagaggagg tcatcttttc gagcattccc
     2581 cccgcaaccc cgtcattcag ccaaatcacc gtattgccga agacggtaaa ccacaaaacg
     2641 ccgaacaggc cggggatgag caaaaccccg aagacaaact cgcggatggt gcgccccttt
     2701 gaaatgcgcg cgataaacaa acccacaaac ggcgcccaag aacaccacca cgcccaataa
     2761 agcaccgtcc aagattcaaa ccacggcttg tgttcccgtt cgtacgcata agttttaaaa
     2821 ctgaggcgca ccagatttcc gaggtagttc cctatgttgt cgccgaatgc cgacaacagg
     2881 taaacagtgg gacccgccgc caaaacaaaa aacagcagca aaaacgcaag gctcaggttc
     2941 aactcgctca acaccttcac gcccttcccc acgccggata ttgccgaaac gacggcgagg
     3001 gacatgacgg cggcgataat caaaacctgc acgctgaagc tgttttcggc aatccagccc
     3061 atttcctgca atccggcgcc cagttgcgaa gccccgaacc ccaatgtggt gatgatgccg
     3121 aaaaaagtag caagcaacgc cataatatca atggcatcgc cgaaccttcc ggaaattttt
     3181 tctttcaaca ggggataaaa acaagaacgc agggcaagcg gcagcttgta gcggaaaccg
     3241 aaataagcca aagccaatgc aatcgtaccg tacaccgacc aagcgtgaac gccccaatgg
     3301 aacaccgtgt gcagcaatgc ctgctgctgc ctgtgttccg gcgtgccggc cgtaatgtcc
     3361 gaaaaataat gcatcaacgg ctctgccacg ccgaaaaaca tcagacccac gcccatcccg
     3421 gccgcaaaca gcatcgccag ccacgacagg aagccgaatt ccggcacatc ttcatcccgt
     3481 ccgagcctga tgtttcccaa actgctgacc gagagtatca gcaggaaacc cagaaaaatg
     3541 gaaaacgtta aaacataaaa ccagctgaac tcggtaaaaa tgacttcttt tgcccgatcg
     3601 agccacatct gcacctgatt cggcacggtt aaaaccaata ccaccaaaac acacacaaaa
     3661 aacaaagtcg tcaaaataac catcggatta aatgacgttc ggcgttctat aaattcagac
     3721 agggacaaac cttctcactc ctttgttaaa aacagacaaa cccggtcatc gggcaaagcg
     3781 gtcaaaacct gccggtaaaa taccggcttc cggatgacga aatgcacaaa ccggccgaaa
     3841 ttgttataat cgaaaaaatt aaaaatcaat acggattatt ctatcagatt gatttatcga
     3901 tatttattat atcattaata tagtttgatt tcaaaccggc ggaaaatgcg ccgcccaccg
     3961 caggcgcgcc ctccccgcta tcggggcgtt taacgccgga ttgccgatgc ggtacaatgg
     4021 ctgatatgaa gaaaattacc cctcaaaacc tgcgccccct gctttcggaa agcttgggac
     4081 ataccgattt tgtcaacgtc ctcaacgcac tgattaaatt tttgcgccgt ggcggcaaaa
     4141 aatgtgcggg ggaacgtttc gacctgatta tcgacacatt caaacaagac agggaattac
     4201 tgtcccgctt cagccggtgt ttttacatct ggctcgcgca aatacacatt tatccggcac
     4261 tcatcaaact cggcatcttc tcgcgccaca gctttgcccg ggaaatgggc atacgcatct
     4321 acgaacgctt cagcccgtca tataaagatt ttgccaactt gggcgaagtc ttcctttatc
     4381 ttttccattc cgaaaacgac gacaaatggc tgcaaacgct caatatccgc caatggctgg
     4441 ttttatacga actcatccgg agccacgccg agccgtccaa attgcagacg gcgggcatcc
     4501 gccttgccga tgcgcgtttg cgcgccatcg aaatgctgtc tgtctggacg gcatccgaag
     4561 ccatcgaacc cgacctcatc cgcatcgccc cgcgcctgct ggaagccgat tcttccttcg
     4621 tcgccctcca acgcgaaacc gccaaactgg tcgaacacta ccgcaacggc accacgcctt
     4681 acgacaccgc ccacctcgaa gtgatgttcg accaatgttt cagccagatt gactatttgc
     4741 gccgcaaagg gacgggcgcc ggctccggtt cgtcggtcaa agtcgcccac ctgctcgaac
     4801 ggctccggca gaccgtagac cgtctaaagc tgctcaccga catccaaacc ggtgccggca
     4861 acagcaaccg cctgaccatc gcgctgatga actccctcat ctacgcggcg gtcgaacaat
     4921 acagcacccg ccacctgcgc cgcagcagca tccgtatgct cgcccgcagc attaccgaaa
     4981 acaaaagcca ccacggcgaa cactacatca cccgcaaccg caaagaatat ttcaaaatgt
     5041 tctactcggc ggcaggcggc ggcatcatca tcgccctgat ggcgctgctc aaaatccgca
     5101 tcggcacact cggcctcagc cccttcctga cttccttgtc ggctgggttc aactacggca
     5161 tcggctttat gatcatccat atgctgcact gcaccgtcgc caccaaacag cccgcgatga
     5221 ctgccgccag ctttgccgaa caggtcgatc tcaacgaagg cggcaaagcg gtggacaaca
     5281 aactcgccaa gctcctcatc gacgtatgcc gttcccaaag tgtcgccgtt ttcggcaacg
     5341 tttccatcgc catccttttg gcgtgcgcca tatcgttcgg ctatgcccat ctgtaccggc
     5401 agcccatact cgatgcccac accgccgcct accagttcaa atccatagac atcatcgctt
     5461 acccgacgct gtggtatgcc gccattgcag gtctgtggct gttctgctcc ggcatcatcg
     5521 caggtttttt cgacaaccgc gccgactacc tcaacctgcg ccaacgcctg cccttcaacc
     5581 ccttgctgcg taaaatcatg cgccccgggc cccgccgcgt cctcgccgcc tacatccaca
     5641 aacactacgg ctcgttggca ggcaacttca tcttcgggat gctcttgggt atgaccggct
     5701 atttcggaca cctcctcggg ctgccgctgg acatccgcca cgtcgccttt tcctccgcca
     5761 acctcggcta tgccgccgtc agcggcaacg tcggtttggg cacgttcgta ctcggcattt
     5821 tcagcgtcct cgccatcggc ctggtcaacc tctgcgtcag cttcagcctc gccctcttcg
     5881 tcgccctgcg ctcgcgcggc acgaaaatcg gcagcatccg caatctgatt aagagttttt
     5941 ggaatcagat taaaagcaat ccctgcatac ttttcctccc gcccgccaaa gaacagggac
     6001 atcctccttc ggacaagcct tgaccggcaa tgccgtctga agcgggattc gccccgaata
     6061 ccgccctgat gcgggaaatc cccataaa
//
LOCUS       DAWHXZ010000126         6018 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000126 DAWHXZ010000000
VERSION     DAWHXZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.126"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..413)
                     /locus_tag="WIE35_001456"
     CDS             complement(<1..413)
                     /locus_tag="WIE35_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ4154361.1"
                     /translation="MLCPEKMSGMAGQYPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASVGA"
     gene            complement(449..562)
                     /gene="rrf"
                     /locus_tag="WIE35_001457"
     rRNA            complement(449..562)
                     /gene="rrf"
                     /locus_tag="WIE35_001457"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(659..3550)
                     /locus_tag="WIE35_001458"
     rRNA            complement(659..3550)
                     /locus_tag="WIE35_001458"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3956..4031)
                     /locus_tag="WIE35_001459"
     tRNA            complement(3956..4031)
                     /locus_tag="WIE35_001459"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3996..3998),aa:Ala,seq:tgc)
     gene            complement(4037..4113)
                     /locus_tag="WIE35_001460"
     tRNA            complement(4037..4113)
                     /locus_tag="WIE35_001460"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4077..4079),aa:Ile,seq:gat)
     gene            complement(4215..5755)
                     /locus_tag="WIE35_001461"
     rRNA            complement(4215..5755)
                     /locus_tag="WIE35_001461"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1314 a         1767 c         1288 g         1649 t
ORIGIN      
        1 gcgccgaccg aagcctgatc cttcagcttc gccagcaccg cagggccgat gccctttacc
       61 ttggtcaaat cgtccacaga cttgaacgca ccgttttgcg cacggtattc cgcaatggcc
      121 ttcgccttcg cagggcctat gcccggcagc gcctccaact cctgctgcga agccgcattg
      181 atgtttaccg ccgcaaggga gaaggcgcag gagaacagca tacagaacag cacgaacatt
      241 ttcttcatgg tttttccttt aagggttgca aacaataaac cgcatcttgc gacgataaaa
      301 cgagtcattc taaaatgaat atcccaaagt ttcaagccgt tcctccgcaa acccgaccgg
      361 acgccgtacg gatactgtcc cgccatcccc gacatttttt ccgggcaaag caaaaacccc
      421 cgaataatcg ggggttttct gaaagggtgt ttggcagtga cctactttcg catggaagaa
      481 ccacactatc atcggcgctg agtcgtttca cggtcctgtt cgggatggga aggcgtggga
      541 ccaactcgct atggccgcca aacttaaact gttacaaatc ggtaaagcct taatcaatat
      601 attcggtaat gactgaatca gtcagtaagc ttttatctct tgaagttctt caaatgatag
      661 agtcaagcct cacgagcaat tagtatgggt tagcttcacg cgttaccgcg cttccacacc
      721 ccacctatca acgtcctggt ctcgaacgac tctttagtgc ggttaaaccg caagggaagt
      781 ctcatcttca ggcgagtttc gcgcttagat gctttcagcg cttatctctt ccgaacttag
      841 ctacccggct atgcaactgg cgttacaacc ggtacaccag aggttcgtcc actccggtcc
      901 tctcgtacta ggagcagccc ccgtcaaact tccaacgccc actgcagata gggaccaaac
      961 tgtctcacga cgttttaaac ccagctcacg taccacttta aatggcgaac agccataccc
     1021 ttgggaccga ctacagcccc aggatgtgat gagccgacat cgaggtgcca aactccgccg
     1081 tcgatatgaa ctcttgggcg gaatcagcct gttatccccg gagtaccttt tatccgttga
     1141 gcgatggccc ttccatacag aaccaccgga tcactatgtc ctgctttcgc acctgctcga
     1201 cttgtcggtc tcgcagttaa gctacctttt gccattgcac tatcagtccg atttccgacc
     1261 ggacctaggt aaccttcgaa ctcctccgtt acgctttggg aggagaccgc cccagtcaaa
     1321 ctgcctacca tgcacggtcc ccgacccgga tgacgggtct gggttagaac ctcaaagaca
     1381 ccagggtggt atttcaagga cggctccaca gagactggcg tctctgcttc aaagcctccc
     1441 acctatccta cacaagtgac ttcaaagtcc aatgcaaagc tacagtaaag gttcacgggg
     1501 tctttccgtc tagcagcggg tagattgcat cttcacaacc acttcaactt cgctgagtct
     1561 caggaggaga cagtgtggcc atcgttacgc cattcgtgcg ggtcggaact tacccgacaa
     1621 ggaatttcgc taccttagga ccgttatagt tacggccgcc gtttactggg gcttcgatcc
     1681 gatgctctca catcttcaat taaccttcca gcaccgggca ggcgtcacac cctatacgtc
     1741 cactttcgtg ttagcagagt gctgtgtttt taataaacag tcgcagccac ctattctctg
     1801 cgaccctcca gggcttacgg agcaagtcct taaccttaga gggcatacct tctcccgaag
     1861 ttacggtatc aatttgccga gttccttctc ctgagttctc tcaagcgcct tagaattctc
     1921 atcctgccca cctgtgtcgg tttgcggtac ggttcaattc aaactgaagc ttagtggctt
     1981 ttcctggaag cgtggtatcg gttacttcat gtccgtagac actcgtcatc acttctcggt
     2041 gttaagaaga cccggatttg cctaagtctt ccacctaccg gcttaaacaa gctattccaa
     2101 cagcttgcca acctaacctt ctccgtcccc acatcgcatt tgaatcaagt acaggaatat
     2161 taacctgttt cccatcgact acgcatttct gcctcgcctt aggggccgac tcaccctacg
     2221 ccgatgaacg ttgcgtagga aaccttgggc tttcggcgag cgggcttttc acccgcttta
     2281 tcgctactca tgtcaacatt cgcacttctg atacctccag cacactttac aatgcacctt
     2341 catcggccta cagaacgctc ccctaccatg ccagtaaact ggcatccgca gcttcggtta
     2401 tagatttgag ccccgttaca tcttccgcgc aggacgactc gaccagtgag ctattacgct
     2461 ttctttaaat gatggctgct tctaagccaa catcctggct gtctgggcct tcccacttcg
     2521 tttaccactt aatctatcat tggggacctt agctggcggt ctgggttgtt tccctcttga
     2581 caacggacgt tagcacccgc tgtctgtctc ccgaggaacc acttgatggt attctgagtt
     2641 tgccatgggt tggtaagttg caataacccc ctagccataa cagtgcttta cccccatcag
     2701 tgtcttgctc gaggcactac ctaaatagtt ttcggggaga accagctatc tccgagtttg
     2761 tttagccttt cacccctatc cacagctcat ccccgcattt tgcaacatgc gtgggttcgg
     2821 tcctccagta cctgttacgg caccttcaac ctggccatgg atagatcact cggtttcggg
     2881 tctacaccca gcaactcatc gccctattaa gactcggttt ccctacgcct cccctattcg
     2941 gttaagctcg ctactgaatg taagtcgttg acccattata caaaaggtac gcagtcacac
     3001 ctctagggcg ctcccactgt ttgtatgcat caggtttcag gttctgtttc actcccctcc
     3061 cggggttctt ttcgcctttc cctcacggta ctggttcact atcggtcgat gatgagtatt
     3121 tagccttgga ggatggtccc cccatattca gacaggattt cacgtgtccc gccctacttt
     3181 tcgtacgctt agtaccgctg ttgagatttc gaatacggga ctgtcaccca ctatggtcaa
     3241 gcttcccagc ttgttcttct atctcgacag ttattacgta caggctcctc cgcgttcgct
     3301 cgccactact tgcggaatct cggttgattt cttttcctcc gggtacttag atggttcagt
     3361 tctccgggtt cgcttctcta agtctatgta ttcaacttag gatactgcac agaatgcagt
     3421 gggtttcccc attcggacat cgcgggatca ttgctttatt gccagctccc ccgcgctttt
     3481 cgcaggctta cacgtccttc gtcgcctatc atcgccaagg catccacctg atgcacttat
     3541 tcacttgact ctatcatttc aagaacttct ttgactttgc ctaacattcc gttgactaga
     3601 acatcagact tgaatttcct actttgataa agcttactgc tttgttgtgt cttaatcctg
     3661 ccttttgtgt ttcaggatta agtcgataca atcatcaccc aaatactgtg tttgttttct
     3721 tttctctcgc gagagatttt tatcctttgc aaagaataaa aaatcaaaac aaacgctttg
     3781 tctttgtttg ttgatttcgg ctttccaatt tgttaaagat cgatgcgttc gatattgcta
     3841 tctactgtgc aaatcaaaac gagctgatta ttatatcagc attttgttct tggtcaagtg
     3901 tgacgtcgcc ctgaatggat tctgttccat tcttccgttt tgatttgtac agtattggtg
     3961 gaggcaaacg ggatcgaacc gatgaccccc tgcttgcaaa gcaggtgctc taccaactga
     4021 gctatgcccc cgttcttggt gggtctggga ggacttgaac ctccgacccc acgcttatca
     4081 agcgtgtgct ctaaccagct gagctacaaa cccggattct cttcttaagc gaatcttgcc
     4141 ttcactcaag cttcttccgc atctttttca gtttaccgat aagtgtgaat gcctaaagcc
     4201 tcttctttct ctagaaagga ggtgatccag ccgcaggttc ccctacggct accttgttac
     4261 gacttcaccc cagtcatgaa gcataccgtg gtaagcggac cccttgcggt taccctacct
     4321 acttctggta tcccccactc ccatggtgtg acgggcggtg tgtacaagac ccgggaacgt
     4381 attcaccgca gtatgctgac ctgcgattac tagcgattcc gacttcatgc actcgagttg
     4441 cagagtgcaa tccggactac gatcggtttt gtgagattgg ctccgcctcg cggcttggct
     4501 accctctgta ccgaccattg tatgacgtgt gaagccctgg tcataagggc catgaggact
     4561 tgacgtcatc cccaccttcc tccggcttgt caccggcagt ctcattagag tgcccaactg
     4621 aatgatggca actaatgaca agggttgcgc tcgttgcggg acttaaccca acatctcacg
     4681 acacgagctg acgacagcca tgcagcacct gtgttacggc tcccgaaggc actcctccgt
     4741 ctctggagga ttccgtacat gtcaagacca ggtaaggttc ttcgcgttgc atcgaattaa
     4801 tccacatcat ccaccgcttg tgcgggtccc cgtcaattcc tttgagtttt aatcttgcga
     4861 ccgtactccc caggcggtca atttcacgcg ttagctacgc tactaagcaa tcaagttgcc
     4921 caacagctaa ttgacatcgt ttagggcgtg gactaccagg gtatctaatc ctgtttgcta
     4981 cccacgcttt cgggcatgaa cgtcagtgtt gtcccaggag gctgccttcg ccatcggtat
     5041 tcctccacat ctctacgcat ttcactgcta cacgtggaat tctacctccc tctgacacac
     5101 tcgagtcacc cagttcagaa cgcagttccc gggttgagcc cggggatttc acatcctgct
     5161 taagtaaccg tctgcgcccg ctttacgccc agtaattccg attaacgctc gcaccctacg
     5221 tattaccgcg gctgctggca cgtagttagc cggtgcttat tcttcaggta ccgtcatcag
     5281 ccgctggtat tagcaacagc cttttcttcc ctgacaaaag tcctttacaa cccgaaggcc
     5341 ttcttcagac acgcggcatg gctggatcag gcttgcgccc attgtccaaa attccccact
     5401 gctgcctccc gtaggagtct gggccgtgtc tcagtcccag tgtggcggat catcctctca
     5461 gacccgctac tgatcgtcgc cttggtaggc ctttacccca ccaactagct aatcagatat
     5521 cggccgctcg aatagcgcaa ggcccaaagg tcccctgctt tctctctcaa gacgtatgcg
     5581 gtattagctg atctttcgat cagttatccc ccactactcg gtacgttccg atatgttact
     5641 cacccgttcg ccactcgcca cccgagaagc aagcttctct gtgctgccgt ccgacttgca
     5701 tgtgtaaagc atgccgccag cgttcaatct gagccaggat caaactctta tgttcaatct
     5761 ctaacttttt aacttctggt ctgcttcaaa gaaaccaaca ggacaatgtt caaaacatta
     5821 tcttgtctgt ctttcaaaca gtgtgaggct caaggcactc acacttatcg gtaatctgtt
     5881 ttgttaaaga gcgttgcgaa ttataaagta tctcttccgc ctgtcaagat atctctcgat
     5941 atccccaaca ttctgtgcta tacttttcag ttcgtccgcc gcttctgcag cagcgaagaa
     6001 ccgaactata cgcccaca
//
LOCUS       DAWHXZ010000127         5992 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000127 DAWHXZ010000000
VERSION     DAWHXZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.127"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(141..728)
                     /locus_tag="WIE35_001462"
     CDS             complement(141..728)
                     /locus_tag="WIE35_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ4154362.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            891..2297
                     /gene="dnaB"
                     /locus_tag="WIE35_001463"
     CDS             891..2297
                     /gene="dnaB"
                     /locus_tag="WIE35_001463"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ4154363.1"
                     /translation="MNDYTAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
     gene            2599..3267
                     /locus_tag="WIE35_001464"
     CDS             2599..3267
                     /locus_tag="WIE35_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ4154364.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKGGAPNNKCDFGKKGQGMLAFGDKNGNK
                     TYDGDTADVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTADRVVWTFNQNGTFGYST
                     NQDLTNTSRFVYSDGYIQIVLTDARAVSDADKKFRSAVVLINSSGRVEVCPRNDRRTV
                     CQYK"
     gene            3297..3917
                     /gene="pilV"
                     /locus_tag="WIE35_001465"
     CDS             3297..3917
                     /gene="pilV"
                     /locus_tag="WIE35_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ4154365.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNNTLSHTLSAVDGEFA
                     IDAIKTKTQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGNAPTFSGNAFSSNCDN
                     KANGDTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGRE"
     gene            3914..4867
                     /locus_tag="WIE35_001466"
     CDS             3914..4867
                     /locus_tag="WIE35_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ4154366.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHIEQDVVSDVTQKDSL
                     FSLKRNSTRNSTNKLIPIAESSNIGYQGFIQRLNALIFQYGIDDVNASADTTVVSSCA
                     AISKPGKQILNLEDVKKELKIVSQDKERNGNIARQRHVVNAYAVGRIAGEEGLFRFQL
                     NEKGEWGNPQLLVKKINKMDIRYIYVSGCPEDDDAGKEETFRYTDKFNSAQDAVTPAG
                     VEVLLSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            4846..5439
                     /locus_tag="WIE35_001467"
     CDS             4846..5439
                     /locus_tag="WIE35_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ4154367.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRSCPANSTNLCIDNKGAKYKKGTQSVSKMPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            5444..5917
                     /locus_tag="WIE35_001468"
     CDS             5444..5917
                     /locus_tag="WIE35_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ4154368.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVDFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASAQAHLETLSSDAGCEAFSNRKK"
BASE COUNT         1651 a         1312 c         1537 g         1492 t
ORIGIN      
        1 tgacagtaac cgtagcaact gaaccgtcat tcccacggaa gtgggaatct agaaataaaa
       61 agcagcaggc atttatcgga aataactgaa attcaatacc gcaaaaatct acccgaaatg
      121 atatagcgga ttaacaaaaa tcaggacaag gcggcaaaac gtttggcgac ttcgtcccag
      181 ttgacgattt cccagaaacc tttcaggtag ttaggacggc tgttgcggta gtcgatgtag
      241 taggcgtgtt cccacacgtc gcaggtcagc agcggcgtgt tttcagtggt cagcggcgta
      301 gcggcgttgg aagtggaaac caaatccaat ccgccggcag gggtttttac cagccacgcc
      361 caaccggagc cgaaagtacc ggccgcgcag gcattgaacg cttcttggaa tttctcgaag
      421 ctgccccatt tcgcgtcgat ggcggcggcc agttcgccgg aaggtttgcc ttgacctttg
      481 ggcgtgaaac ccagccagta gaaagtgtgg ttccaagttt gggctgcgtt gttgaacacg
      541 ccgcccgaag attttttcac aatctcttcc aaaggcaggt tttcaaattc ggtgcctttg
      601 atttgattgt tcaggttggt gatgtaggtt tgatggtgtt tgccgtagtg gaattccaaa
      661 gtttctttgc tcagatgcgg ggacaatgcg tccagttcat aaggcagttg cggcagctta
      721 tgttccattt tgtgctccta atattgtttt aaatgttgta ttttggcagt gttgctgcaa
      781 atagctcagc agcgcacata ttttacctgt tttgagggat ggaaaccaat taaacctgct
      841 ttacgctata atagaaggtt gcaatttcgg cacgacagat aggatgtacc atgaacgatt
      901 acacagctat gccgtctgaa gaccgtgagg tcggcgcatt gtcgctgcct ccacattcga
      961 tggaggcgga acaatccgtt ttgggcggtt tgatgctgga aaatccggct tgggacagga
     1021 ttgccgatgt ggtttccggt gaagacttct accgccacga acaccgcctg attttccgat
     1081 ccattgctaa attaatcaat gagagccgtc ccgccgatgt gattacggtt caggaagatt
     1141 tgcagcggaa cgaagaattg gaagcggcag gcggattcga atatctgatt acgctggcgc
     1201 aaaacacccc gtctgccgcc aacatccgcc gctacgccga aatcgtgcgc gagcgttcca
     1261 tcatgcgcca actcgccgaa gtggggacgg aaatcgcccg cagcgcatac aatccgcaag
     1321 gcagggacgc ggggcagctt ttggacgagg cggaaaacaa agtattccaa atcgccgaaa
     1381 gcaccgccaa atccaagcag ggctttttgg agatgcccga tttgctgaaa gaagtcgtac
     1441 agcgcatcga tatgctctac tcgcgcgaca atcccgatga agttaccggc gtgccgacag
     1501 ggttcatcga cctcgacaaa aaaacctcgg gtctgcaacc cggcgacctg attatcgttg
     1561 ccggtcgtcc gtctatgggt aagaccgcct tttctatcaa tatcgccgaa cacgttgccg
     1621 tagaaggcaa gctgccggtt gccgtcttct caatggaaat gggcggggcg caactggtca
     1681 tgcgtatgct cggctcggtc ggacggttgg atcaaagcgt tttgaaaacc ggcaggctcg
     1741 aagacgaaca ctggggtcgc ctgaacgaag cagtcgtcaa actctccgac gcgcccgtgt
     1801 acatcgacga gaccccgggt ctgaccgcgc tcgaactgcg cgcccgtgcc cgccgtctcg
     1861 cccgtcaatt taacaataag cttggattga ttgtcatcga ctacctgcaa ctgatggcag
     1921 gatccggccg ttccgacaac cgagcttcgg agctgggaga gatttcacgt tcgctcaaag
     1981 cgttggcgaa agaattgcaa gtccccatca tcgccctgtc gcaattgagc cgcacggtcg
     2041 aatcgcgtac cgacaaacgc ccgatgatgt ccgaccttcg cgaatccggt gctatcgagc
     2101 aggatgccga cctgattatg tttatgtacc gcgacgaata ctacaaccaa gactcgccca
     2161 tgaaaggcct tgccgaatgt atcatcggca aacaccgcaa cggtcccgtc ggtaaaatct
     2221 tcctcacatg gacgggacaa ttcaccaaat tcgacaatgc tgcctatatt cccgaggagg
     2281 caaagataga ggattaaatg gctatataaa aatttattag gcgaaatcag gcaaaatcgt
     2341 ttaaaatcat gcttagagat tgccctaaaa aataaaacgc ggtcttgagg catttttgca
     2401 ttcagcccgc atataattga aaatatagtg gattaacaaa aatcaggaca aggcaacgaa
     2461 gccgcagacc gtacaaatag tacggaaccg attcacttgg tgcttgagca ccttagagaa
     2521 tcgttctctt tgagctaagg cgaggcaacg ccgtactggt ttgaatttaa tccacgatac
     2581 attaccagtt aacgtcctat gtgtacacga aaacaacaag gtttcacgct aacagagctg
     2641 ctcatcgtga tggtcattgc agccattatg gcgatgatag ccctccccaa tatgagccaa
     2701 tggattgcat cccgccgcat tgccagtcat gcggagcgga ttgccaacct tttgcgtttc
     2761 tccaggggag aagccgtccg gctcaatctt cctgtctata tctgtcctgt tcaagttaaa
     2821 aaaggcggtg cgcccaacaa taaatgtgac ttcggcaaga aggggcaggg aatgttggct
     2881 tttggcgata agaatggcaa taagacatat gacggcgata cggcggatgt tctccttcgc
     2941 agtgtggtat tgaatgatga tatcaatgat aagcggatta attatgcctt taaccatatc
     3001 gctttcggtc aaacgcagcc gaccgctgac cgtgtagttt ggacattcaa tcaaaacggg
     3061 acgttcggtt attctaccaa tcaggatctc acgaatactt ccagatttgt ttactccgac
     3121 ggttatatcc aaatcgtgct gacagatgcg agagcagttt cagatgccga taagaaattc
     3181 cgttcggcgg tggttttgat taacagcagc ggcagggttg aggtttgtcc tagaaacgat
     3241 aggcgtaccg tatgccaata taaatgattg ttttagtcgg tttgggaaat aaaaaaatga
     3301 agaataatga ttgcttccgc ctgaaaaacc cccagtccgg tatggcgctg atagaagtct
     3361 tggttgctat gctcgttctg accatcggta ttttggcgtt actgtctgtg cagttgcgga
     3421 cagtcgcttc cgtcagggag gcggagacac aaaccatcgt cagccaaatc acgcaaaacc
     3481 tgatggaagg aatgttgatg aatccgacca ttgattcgga cagcaacaag aaaaactata
     3541 atctttacat gggaaacaat acactatcgc atacactatc agctgtggat ggtgagtttg
     3601 cgattgatgc cataaaaact aagacacagt tggcagaggc acaattgaag agatttagtt
     3661 atgagctgaa aaatgccttg ccggatgcgg cagccatcca ttacgccgtc tgcaaggatt
     3721 cgtcgggtaa cgcgccgaca ttctccggca atgctttttc ttcaaattgc gacaataagg
     3781 caaacgggga tactttaatt aaagtattgt gggtaaatga ttcggcaggg gattcggata
     3841 tttcccgtac gaatcttgag gtgagcggcg acaatatcgt atatacctat caggcaaggg
     3901 tcggaggtcg ggaatgagac gtaaaatgct aaacgtacca aaaggcagtt atgatggtat
     3961 gaaaggtttt accattattg aatttttggt tgcgggcatg ctcagtatga ttgtcctgat
     4021 ggcggtcgga tcgagttact tcacatcccg gaaattaaat gatgcggcaa acgagcgtct
     4081 tgctattcaa caggatttgc ggaatgcggc aacattaatt gtccgcgatg caagaatggc
     4141 ggggagcttc ggttgtttca atatgtctga gcatatcgaa caggatgttg tttccgatgt
     4201 gacgcaaaaa gactctcttt tttccttaaa aaggaacagt acaaggaaca gtacaaataa
     4261 acttatcccc atagcggaat cttcaaatat cggatatcag ggttttatcc agcgccttaa
     4321 cgcattgatt ttccaatacg gaatcgatga tgttaatgca agcgccgata ctaccgtcgt
     4381 cagcagctgt gccgcaatat cgaaaccggg taagcaaatc cttaatttag aagatgtaaa
     4441 aaaagaattg aagattgtga gtcaggataa ggagcgaaat ggcaatatag cgcgtcaaag
     4501 gcatgtggtc aatgcctatg cggtcggcag gattgccggt gaggaaggtt tgttccgctt
     4561 ccaattgaat gaaaagggcg agtggggtaa tcctcagttg ttggttaaaa agattaataa
     4621 aatggatata cggtatattt atgtttccgg ctgtcctgaa gatgacgatg ccggcaaaga
     4681 ggaaacattc agatatacgg ataaattcaa cagcgcccaa gatgctgtta cgcctgccgg
     4741 ggtggaggtt ttattgagta gcggtactga taccaagatt gccgcttctt cagacaatca
     4801 tatttatgct taccgtatcg atgcgacaat acgcggggga aatgtatgcg caaacagaac
     4861 actttgacgg gaatcccgac ttctgacgga cagagggggt ttgcactgtt tatcgtgctg
     4921 atggtgatga tcgtcgtggc ttttttggtt gtaactgccg cgcagtctta caataccgag
     4981 cagcggatca gtgccaacga atcagacagg aaattggctt tgtctttggc cgaggcggct
     5041 ttgcgggaag gcgaacttca ggttttggat ttggaatatg atacggacag taaggttaca
     5101 tttagcgaaa actgtggaaa aggtctgtgt accgcagtga atgtgcggac aaataatgat
     5161 aatgaagagg cttttgacaa tatcgtggtg caaggcaagc ccaccgttga ggcggtgaag
     5221 cgttcttgcc ctgcaaattc taccaacctg tgcattgaca ataaaggggc gaaatataag
     5281 aaaggcacgc aaagcgtcag caaaatgcca cgttatatta tcgaatattt gggcgtgaag
     5341 aacggagaaa atgtttatcg ggttactgcc aaggcttggg gtaagaatgc caataccgtg
     5401 gtcgtccttc aatcttatgt aagcaataat gatgagtaat aaaatggaac aaaaagggtt
     5461 tacattgatt gagatgatga tagtcgtcgc gatactcggc attatcagcg tcattgccat
     5521 accttcttat caaagttata ttgaaaaagg ctatcagtcc cagctttata cggagatggt
     5581 cggtatcaac aatatttcca aacagtttat tttgaaaaat cccctggacg ataatcagac
     5641 catcaagagc aaactggaaa tatttgtctc aggctataag atgaatccga aaattgccga
     5701 aaaatataat gtttcggtgg attttgtcaa taaggaaaaa ccaagggcat acagcttggt
     5761 cggcgttcca aagacgggga cgggttatac tttgtcggta tggatgaaca gcgtgggcga
     5821 cggatacaaa tgccgtgatg ccgcttctgc ccaagcccat ttggagacct tgtcctcaga
     5881 tgccggctgt gaagccttct ctaatcgtaa aaaataaggt tgttttgcca ataccgtctg
     5941 aaaatcaatg ttcagacggt atttttatgg gtatagtgga ttaacaaaaa tc
//
LOCUS       DAWHXZ010000128         5986 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000128 DAWHXZ010000000
VERSION     DAWHXZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.128"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(241..3093)
                     /gene="gcvP"
                     /locus_tag="WIE35_001469"
     CDS             complement(241..3093)
                     /gene="gcvP"
                     /locus_tag="WIE35_001469"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ4154369.1"
                     /translation="MKLSELFNPDEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNMINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDERVYPQTLDVMKTRAKYFGFELVVGDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVSADIMSLVLLKPPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDAFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIAKRIHALASAFADALVSDGIKVVHKVFFDTVTVDFGSK
                     EKTDQVFAAALESGYNLRRVNDTQVAAAFHETSTREDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQNDILQHPVFNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLSIRRYQEANGEGHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAKVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASADQT
                     AVAAAAYGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLGEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKEDNPLVNAPHTASDVTGEWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3124..3483)
                     /locus_tag="WIE35_001470"
     CDS             complement(3124..3483)
                     /locus_tag="WIE35_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154370.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRCANLMAVLPL
                     FVVILVLLNIVFPLPAHPLAWLVPAGFMVLVGGFPLSLPLAVLLVLTCCILAHCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            3431..3622
                     /locus_tag="WIE35_001471"
                     /pseudo
     CDS             3431..3622
                     /locus_tag="WIE35_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
     gene            complement(3758..4399)
                     /locus_tag="WIE35_001472"
     CDS             complement(3758..4399)
                     /locus_tag="WIE35_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ4154371.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4389..4940)
                     /locus_tag="WIE35_001473"
     CDS             complement(4389..4940)
                     /locus_tag="WIE35_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ4154372.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFGEADIERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(4944..5462)
                     /locus_tag="WIE35_001474"
     CDS             complement(4944..5462)
                     /locus_tag="WIE35_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ4154373.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5462..>5986)
                     /locus_tag="WIE35_001475"
     CDS             complement(5462..>5986)
                     /locus_tag="WIE35_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein B"
                     /protein_id="HEZ4154374.1"
                     /translation="ISSNNLLTGSKMIELNDQPSASPKLRPHTVYAGDTVIATQGGGL
                     DDLQVKLADLLDKFDKLPLDKTVAELNGSLAELKSTLKSANAALSSIDKLVGKPQTQN
                     IPNELNQTLKELRTTLQGVSPQSPIYGDVQNTLQSLDKTLKDVQPVINTLKEKPNALI
                     FNSSSKDPIPKGSR"
BASE COUNT         1249 a         1504 c         1741 g         1492 t
ORIGIN      
        1 gtttgtccgt gtggaaatga catcaaaggg tgtgaatagg cttttcatct ttgaaatctg
       61 tctgcccgat cggatgaata aaaagtagga tgataaaaaa aactgatgta tggatactgg
      121 tttaacccat ttgttttaaa gttatagtcg agatacgtgt atcggtgcaa cgagtctgcc
      181 gtcgacgaaa atgccgtctg aaagtgtttc agacggcatt tgtctttggg gtttcaacgg
      241 tcagtcttca taattttcca tcggtgggca gctgcacacg agattgcggt cgccgtacac
      301 gtcgtccacg cggttgacga agggccagaa tttgtgttcg cgcacgaagg gcagcgggaa
      361 gacggcttct tcgcgagagt acggatgcgc ccattcgccg gttacgtcgg aggcggtatg
      421 cggagcattc accagtgggt tgtcctcttt cggccattcg ccgtcgatga ctttctgcac
      481 ttcgcggcga atggatttca gggcggcgat gaagcggtcg agttcggctt tgctttcgct
      541 ctcggtcggc tcgatcatca gcgtaccggc aacagggaag gaaaccgtcg gcgcgtggaa
      601 gccgtagtcc ataaggcgtt tggcgatgtc ggtttcggtg atgccgcttt cggctttgag
      661 cggacgcaaa tcgacgatgc attcgtgcgc gacgcggccg tttttgccgg tgtagagaat
      721 cggatagtct tcgcctagac gtttggcgac gtagttggcg ttgagcaatg cccaacgtgt
      781 cgcctgctcc ataccttgtt tgcccatcat ggtcaggtac atccaagtaa tcggcaggat
      841 ggatgcagaa ccatatgctg cggcagccac ggcagtttga tcggcacttg cgctgtgggt
      901 gtcggtcaag gtatgacccg gggcaaacgg ggcgaggtgg gctttcagac cgatgggacc
      961 catgcccgga ccgccgccgc cgtgggggat gcagaaggtt ttgtgcaggt tcatgtgcaa
     1021 cacgtctgcg ccgactttgg caggctgcat gatgccgatt tgggcgttga ggttggcgcc
     1081 gtccatgtaa acctgtccgc cgttttcgtg gacgatgcgg cagatgtcgc ggatgccttc
     1141 ttcgtacacg ccgtgggtgg acggataggt aatcatgatg gcggacaaag cgtcgcggtg
     1201 ttgctcggct ttggctttca aatcgtcgat attgacgttg ccgtgttcgt cggtatcgac
     1261 aacgacgact ttcaagccca gcatggccgc agtcgcaggg ttggtaccgt gggcggattt
     1321 gggaatcaga cagatgttgc ggtgtccctc gccgttggct tcctgatagc ggcggatgga
     1381 gagcataccg ctgtattcgc cctgcgcgcc ggagttgggc tggaaggaaa tcgcgtcaaa
     1441 gccggtgatg gctttcaggc tgttttccat gtcggcaagc aattcgcggt agccggcggt
     1501 ttgcgcttcg ggagcgtaag ggtggatgtc ggagaactcc gcccaagtaa tcggcaacat
     1561 ttccgcagtg gcattgagtt tcatggtgca actgcccagc gaaatcatac tgcggttcat
     1621 cgccaaatcg cggtcttcga gttttttcag gtagcgcaac atttcgtgtt cggtgtggta
     1681 actgttgaac acaggatgtt gcagaatgtc gttttgacgc agtaattcgg cgttcagacg
     1741 gcctttgaca tcatcggcaa atgcgaacgt atccttgccg gtaaacgcgc ggtacaaatc
     1801 aaccaaatct tcgcgtgtcg aagtttcatg gaaggctgct gcgacttgag tatcgttgac
     1861 gcggcgcagg ttgtaacccg attccaaagc agcggcaaac acttggtctg ttttctcttt
     1921 actgccgaaa tcgacggtaa cggtatcgaa gaagactttg tgaaccactt taatgccgtc
     1981 tgaaaccagc gcgtcggcaa aggcagaagc cagcgcgtga atgcgcttgg caatacgttt
     2041 cacaccttcg gggccgtggt aaacggcata catacccgcc aagttcgcca gcaatgcctg
     2101 tgccgtacaa atattggatg tcgctttttc gcggcggatg tgttgctcgc gggtggacaa
     2161 cgccatgcgc aaggcaggtt tgcccgatgc gtctttggat acgccgatga tgcggcccgg
     2221 ggcggaacgt ttgaacgcgt ctttaaacgc gaaataagca gcgtgcggac caccgaagcc
     2281 catcggtacg ccgaagcgtt gcgtgttgcc caaagcaata tccgcgccca attcggcagg
     2341 cggtttcagc aaaaccaagc tcataatgtc ggcggaaacg gcaacgattg tgccttttgc
     2401 tttcagacgg ccgataacgt cctgcaagtc ttgcacatcg ccgtctttgc cgacgtattg
     2461 gaacagcgcg ccgaagtatt cgccctcgtc ggcttgagca aaatcgccga ccaccagctc
     2521 gaagccgaaa tacttggcac gggttttcat cacgtccaaa gtttgcggat acacgcgctc
     2581 gtccacaaag aaacgctcgg attttacctt gcccacgcgg tgcgccatcg ccatcgcttc
     2641 ggcggcggcg gtcgcttcgt ccagcaaaga cgcgcccgcc acagggaaac cggtcaaatc
     2701 gatacacact tgttggaagt tcaacaaagc ttccaaacga ccctgcgcga tttccgcctg
     2761 atacggcgtg taggcggtgt accaacccgg attttccaat acgttacgca aaatcacgtt
     2821 cggcacgcgg gtcgggtaat agcctaaacc gatataggat ttgttgatca tgtttttcga
     2881 cgcaatgcct ttcaattttg ccaacgcgtc cgcttcggtc agggcatcgg gcaaatcgag
     2941 ttcagacggc atacggatgc tttgcggcac ggtgttgccg acaaaatcgt ccatactttt
     3001 ctcgccgaca gcggcaagca acgccgcttc gtcgccaaaa ctcaaatgcc gcgcggcaaa
     3061 ttcgtcgggg ttgaacagtt cggacagttt catttctact ccttcgggtt tgtagggctg
     3121 ccatcaggca gaatttttag acatcggatg attcgggcaa gggtagcaca aaagacggga
     3181 taatggcgga caatgcgcca gaatgcagca ggtcaggaca agcagcaccg caagcggcag
     3241 cgataaggga aagccgccga ccaaaaccat gaaacctgca ggcaccagcc aagctaaggg
     3301 atgcgccgga agcggaaaaa caatattgag cagaaccaaa atcacgacaa acagcggcag
     3361 caccgccatc aggttggcac acctgtatgt tgcgtacggg tcggacgctt cttcaggttc
     3421 gcaatattcc atgatttcag cctgtccggg ctttatccac agcaggctcg gacggtcggt
     3481 catgccgtct gaaccggcag gccgtctttt tcaaaaaatg ccataccgct gcaacttcat
     3541 gcggctgtat ggcgtaggtt ttgcacttca gacggcatat gcgtcccgaa gcagtttggt
     3601 gtcccagtcg gttcatgatt atagtggatt aaatttaaac cagtacggcg ttgcctcgcc
     3661 ttgccgtact atttgtactg tttgcggctt cgtcgccttg tcctgatttt tgttaatcca
     3721 ctatatataa acaacttggg tgcggataaa cctttgttta tctaaacccc tgttttctca
     3781 aaaattgatt caaatcttcg gcaaatttcc gatagccttt gccgttggcg tggatttggt
     3841 cggatttcag attattatcg cccaaaattt ccgcccacgc gccgccgaac agcggaatgc
     3901 cgtattcctc ggacaaatcc tcatacagcg gatgatcgct caaatgcccg aacaacgcgc
     3961 ccaaggtaat gtgcggcacg ccgacgagga cggcggggat gttttccttc tgcacggttt
     4021 cgatgatttt cgcgatattg gcgcgggtct gctccttggg aactttgcgc agaaagtcgt
     4081 tgccgcctat gccgacaatc acaagcttgg gtttgcgtgc caacagcgcg ggcaggcgcg
     4141 acagggcttg ggcggatgta tcgcccgata cgccgccgtt gacaatattc caacccgtca
     4201 gtttttgcag ttgcgcgggg taggattcgc cggggtttgc gccgtagccg aaggtaagcg
     4261 aatcgcccaa ggcaagtacg gtgcttcctt cgggaatttt ggcgtgggtt cgggcggatt
     4321 ttctgccgca ggcggtaagg agcaacgcgc ctgcgccgag gaggaaggtt cttctgttca
     4381 ttggtttttc agatggcatc gagccatttc gggtggtatt tcagggtttc acggtaaacc
     4441 ggacagcttt cggcgtgcgc gcctttgagg tagccggtac tcatcagaaa ttcgccgacg
     4501 atttcgccgc cgacgaattt gaaatgtttt ttaaagagtt taacccattc gtctttgctt
     4561 cgcggatggt gcgcgtcgag ccagttcttg aacgagccgt attcttgttg caacgcttgg
     4621 atttgccgtg cattgaaaat ggcggcatcg attttcaggc ggttgcggac aatacccgca
     4681 tcggcaagca gccgctcaat atcggcttcg ccaaatgcgg caacagtatc gatgtcgaac
     4741 ccttcaaatg ccgtctgaaa cgcctgccgc ttcttcagca tcagcgtcca gctcaatcct
     4801 gcctgattga tttccaacac cagccgctca aacaattcat tgtcgtcctc aatcggaaaa
     4861 ccgtattggg tgtcgtggta atgtttgttc gggttgtcgg tgttttcggg aagtgaggcg
     4921 gcaaattcgc aatagttcac ggtttactcg accatctgtt gcgccgcctg tttcagtccc
     4981 tgttcgagtg cggcggtcat ggcggcgtag ccgtcaccct gctgttcggt ttcgatatgg
     5041 aagggtctgt tcgtaccgtc gggtaggacg gcgtagccgc tgatgagggt tttgcccgtg
     5101 tagctgcctt ggaatgcgtc gatatagacc gtccattttt cggtactgcc gctgcgtgag
     5161 gcaggaacaa agatgcgtgt gctgtccaaa cggttgaatg cattgctcaa cgccgcttcg
     5221 agcatatcgt ccaaggtgtc tgcccagacg tggttttgtg cggtgttgag gcggtagggg
     5281 tcggtttgat agaccagtcc gccgcgtttg agcggttcgg caagacggac ttcgacggca
     5341 gtttcgccgc cttgcgttgc aggacggatg tagcggctgt cgggcaacac gaaatattgt
     5401 gtgctttgca cagtaccgca ggcggcaagc gtcagggcgg cggcaatcgg gaagaggcgc
     5461 attatcggct tcctttcggg atagggtctt tgctgctgct gttgaaaatc agcgcgttgg
     5521 gtttttcttt caaagtgtta atgacgggtt gaacgtcttt taaggttttg tccaaacttt
     5581 gcagcgtatt ttgtacgtcg ccgtagatag gcgattgagg cgatacgcct tgcagggttg
     5641 tgcgcaactc tttcagggtt tggttcagtt cgttcggaat gttttgtgtc tgcggtttgc
     5701 cgaccagttt gtcgatggag cttagggcgg cattggcaga tttgagtgtg gatttgagct
     5761 cggcaagcga accgttcaat tcggcaaccg tcttatctaa aggcagtttg tcgaacttgt
     5821 ccagcaaatc cgccaatttg acctgcaaat cgtccaaacc gccgccctgg gtcgcgataa
     5881 cggtatcgcc tgcataaacg gtatgcggtc gcagcttagg tgatgcggaa ggctgatcgt
     5941 tcaactcaat cattttgctt ccggtcagca ggttgttgct ggagat
//
LOCUS       DAWHXZ010000129         5974 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000129 DAWHXZ010000000
VERSION     DAWHXZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.129"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..891)
                     /gene="mafA"
                     /locus_tag="WIE35_001476"
     CDS             complement(<1..891)
                     /gene="mafA"
                     /locus_tag="WIE35_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ4154375.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVE"
     gene            1044..1148
                     /locus_tag="WIE35_001477"
                     /pseudo
     CDS             1044..1148
                     /locus_tag="WIE35_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1155..1304)
                     /locus_tag="WIE35_001478"
                     /pseudo
     CDS             complement(1155..1304)
                     /locus_tag="WIE35_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1530..2249)
                     /locus_tag="WIE35_001479"
     CDS             complement(1530..2249)
                     /locus_tag="WIE35_001479"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ4154376.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(2493..3347)
                     /locus_tag="WIE35_001480"
     CDS             complement(2493..3347)
                     /locus_tag="WIE35_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ4154377.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPATVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKKAVDYAA
                     EVAAAAKV"
     gene            complement(3476..4204)
                     /gene="rpsB"
                     /locus_tag="WIE35_001481"
     CDS             complement(3476..4204)
                     /gene="rpsB"
                     /locus_tag="WIE35_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ4154378.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAEGGFNKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(4377..4610)
                     /locus_tag="WIE35_001482"
     CDS             complement(4377..4610)
                     /locus_tag="WIE35_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154379.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFT
                     ITVRRRNHLMRQQEQAESEQQRAQRQKDSGTKP"
     gene            4688..5269
                     /locus_tag="WIE35_001483"
     CDS             4688..5269
                     /locus_tag="WIE35_001483"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ4154380.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINHLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRAAQKRGVKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     AKLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVGTLPREAHDLPLDGFVSEAGILCF"
     gene            5330..5920
                     /locus_tag="WIE35_001484"
     CDS             5330..5920
                     /locus_tag="WIE35_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ4154381.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         1345 a         1351 c         1677 g         1601 t
ORIGIN      
        1 ctctacggat ggggcagagt tacccgtatg attgccgtat ggtcggatat cggagaaatc
       61 gaccattaat ccttccgtcg gtttaattcc tttgcttact ttatacggcc ccatccacaa
      121 tgcgtaattt tctttatagg cagcttcaaa cgcattggtt ttgggtttga tgagcaattt
      181 tttattggtt ctgtctactg cgaaatattc cagttttgtt tgggctttca gtgtttcggc
      241 attgtatagg tgcatttcgg ttctgttgcg tatcgttccg aatacgtcga tgttaataaa
      301 cacatctgta tcggcattgg caggagaaac aacgtctatg ccgcgcagga aaaatacggt
      361 ctgtaccaag tgggaaagaa aggcagtgtc gcgcgggtta gtcgtcaagg tttcatttcg
      421 ataatccccc atcccgccaa tatttaagcc cagactgctt ttacttccgc taccgtctga
      481 ttgggtgcga gagcgtgcag gggcattaag tgtagataaa gaagtggtta aacctgtcaa
      541 accgcctgat gttgtttcag cggtggtttc gtaacgtgga taggtgtaat cggtacggac
      601 ggcagggctg tttatgtatt cgccacgaat cagtgcatca atggagtagc gaccccctgt
      661 caaactgcct gaaccttggt cgcccatagt tgcaatgtac aatgcaactt ttcgtccgtg
      721 taatgcctgt aaatccatgt ctttaacggc agctctggca gaagcggcca caagttcttg
      781 ttcgaccgca aagcgtttac ctccgccatg cgatggaata cctgtcagtg tcccgcaggc
      841 ggataaaata aaaactgaaa aaagaatagg tatcagcagc cgtgcttgca tagattttct
      901 cctttgatga aaaacaaatt gtatcaaaat tgtaaacaaa ttataaatta aataaaatat
      961 ttctttactg gttttgtggt ttggttttca ataggctgcc cgcacatagt gggatttacg
     1021 gttgcaatgg ctgcggcgta ttttatagtg gattaaattt taatcaggac aaggcgacga
     1081 agccgcagac agtacaaata gtacggcaag gcgaggcaac gctgtactgg tttaaattta
     1141 atccactata ggcttttttc ggtcagttgc cggttaatca gtcccggcag ttcggacagg
     1201 gtgttgtctt gcgccaacct gttgcggtag cggcataagg tgctgtaatc ggggatgctt
     1261 ggtttgtcaa aacggcaaaa caggttgaaa tcggtgcggg tgatgggtac ggcgttgccc
     1321 ttatgccgat tttgtccgcc tgctgcaagg cggtttgtct aatgaaagga aaaatgccgt
     1381 ctgaacattg ttcagacggc attttcaaat ggaaggcaat atagtcggca tatgaaaaat
     1441 tgatgtataa ggcaggatgg gtttaggctt gttaatttcc atataataaa tggccgtatg
     1501 aagcccatat gcgactatat ctgccggaat cagcagtgaa ccagtgtacc ctcgttttcg
     1561 ccggttacca cgcgtttgag cgagccttct ttagcgatac cgaatactac gatattgagc
     1621 ttgcgttcgc ggcagagggc gaaagcggtg gcatccatca ctttaaggtt tttcaacaag
     1681 gcttcgtcaa aagtaatggt ttcgtagcgt gttgcagatg ggtctttttt cgggtctgcg
     1741 gtgtacacac cgtcaacgtt ggtggctttg agcatcacgt cgcagttcat ttctgcaccg
     1801 cgcaatgcgg cggcagtgtc ggtcgtgaag aacggattac cggtaccggc ggcgaaaatc
     1861 acgactttgc cttcttccaa atattgaatg gctttaggac gggcgtaagt ttcggcgatt
     1921 tgctgcatag acagtgcgga ttgtacgcgc gctttgatgc ccaaagtttc aaatgcgtct
     1981 ttgagtgcca acgcgttcat tacggttgcc atcatgccca tgtagtcggc ggtggcgcgg
     2041 tccatactgc ctgcttgtgc agatacgcca cggaaaatgt taccgccgcc gacaacgata
     2101 ccgacctgca cgcccatttt aacgacttcg gcaatttcgc cgacagtttg aacgatggta
     2161 tcgtgattga tgccgaacgg atcggaaccc atcagggatt cgccggagag tttcagtaat
     2221 acgcgtttgt atttgatttg ctgtgtcatg ggatattctg ctttctcgtt ggggatgttg
     2281 ccggcttccg ggcgggatgc cgtctgaaag gtgtatgcct tggcagggcg tatgcggcgt
     2341 gccggcggtg tgaaaacggg ctgctgaatt tgccgccgct gttttcgggc ggggcgggca
     2401 tttgaaccct gccgccggaa aatatgggcc tttcccaaaa aaaagcaccc tgattcgttt
     2461 ggaatccagg tgctttcttt ttcataagtg ccttacactt tggcggcagc ggctacttct
     2521 gccgcgtaat cgacggcttt tttctcgata ccgtcgccta ctttgtagcg gacgaagctg
     2581 accacttcag tgccgttttc tttagcgaat tgggcaacag tttggtcggg gttcatcacg
     2641 aacgcttggc cgttcagagt gatttcagcc aagaatttgc ggatacggcc ttcaaccatt
     2701 ttagcggcga tgtcggcagg tttgccggaa gcgatagctt gttcagtgta gatgtggcgt
     2761 tctttttcaa cggtttcagc atctacttcg gcttcactta cgcattgtgg tttagcggca
     2821 acgatgtgca taccgatttt gcgtgctacg tcttcagagc ctttgtactc aaccaatacg
     2881 ccttcggtag ccaaagcacc gtggatgtag gcaaccagtt ggttggcagt gtcgatcact
     2941 tggaagcggc gaacagacat gttctcgccc aatttagcga tgatggcttt acgttctgct
     3001 tcaaccagtt cgctcagttc ttcaacggta gccggttttt tctcggcagc ggttttagca
     3061 acgaagttgg caaattctac gaagcccgcg tctttggcaa cgaagtcggt ttcgcagttt
     3121 acttcgacca atgcgccgac attgccgttg atggcgtaag ctaatacgcc ttcggcagcg
     3181 gtacgaccgg ccagtttacc ggctttcgcg ccggatttga tgcgcaggat ttcttcggct
     3241 ttgtcgaagt tgccttcagc ttcaaccaag gcttttttgc attccatcat gcccaagccg
     3301 gtagcggcgc gcagatcggc aaccattttt gcagtaattt ctgccatttt gaatctccta
     3361 gatttttggg aaatacggaa aggattttcc gcattcggga atcgtgtgcc gcgaacggac
     3421 gttcagacgg catatttgag aaaaggggca taacgcccct cttctgtaca ccgaattact
     3481 cggcagcagc ttcttgggca gcggctacag tttcttgcag cgcttggttt ttgccttcca
     3541 aaactgcgtc ggcgatgccg cggcagtaca ggcggatggc tttggcggag tcgtcgttgc
     3601 cggggataac gtatttcacg ccgtcagggc tgttgttggt atcgactacg gcgataacgg
     3661 ggatgcccaa tttctcagct tcgaccagag tacctttttg gtagccggta tcgataacga
     3721 aaatcgcgtc aggcaggcct ttcatgtttt tgataccgcc caaagaacgt tccagttttt
     3781 caacgtcgcg ttgcatttcc agaatttctt ttttgttgaa accgccttca gcagcgtttt
     3841 ccagggcagc agttttttct tccaggcgtt tgatggattg cttaacggtt ttgtagttgg
     3901 tcagcatacc acccaaccag cggtaatcaa cgaaaggcat accggcacgg gtagcttctt
     3961 cgcggatgat gtcgcgggct tggcgtttgg tacctacgaa caatactgta cctttgttgg
     4021 caaccagacg acgtacggct tcttgcgcct cttggaacat cggcagggtt ttttccaagt
     4081 ttacgatatg gattttgttg cgcgcaccga aaatgtattg tgccattttc gggttccaaa
     4141 aacgggtttg gtggccgaag tgaacaccgg cttcaatcat ctgacgcata gtaatttgag
     4201 acattttatt tccttgaaag ggttaagagc acacattcaa tcgcatagaa cttgataggt
     4261 cacgcctatt gtcaagcacc cttcggcgaa agtgggcata agttttaaaa aatagtgtgt
     4321 ttccgaaatg gaaaactgac ggattatagc ggataagatg ccgtctgaaa agggattcag
     4381 ggttttgtgc cgctgtcttt ttgccgttgt gcgcgctgct gttcggattc cgcctgttcc
     4441 tgctgcctca tcaggtggtt gcggcggcgg acggtaatgg tgaaaaccgt gaacacggtg
     4501 ggcagcaccg cccaaaaaac caaataaatc aaggcgcgcg caatactcgg ctgcgcggcg
     4561 gaatacataa cggcaacaaa aagatagccg atggcgacga tgtgccacat tgtgtgcgtc
     4621 ctgtttgaat gttaacaaaa tcgttataat cgcaatattc taccgcaatc ctgaaaggcc
     4681 gtctgaaatg aggaacgagg aaaaacgcgc cctgcgccgc gaattgcgcg ggcggcgttc
     4741 gcaaatgggg cgggacgtgc gggcggcggc aacggtaaaa atcaaccacc tgctcaaacg
     4801 ttatattaaa aaagggcgga aaatcggcgt gtattggccg atgggcaagg aattgcgttt
     4861 ggacggcttt gtccgcgcgg cgcaaaaacg cggtgtaaaa ctttatctgc cttatatcga
     4921 accgcgttcg cggcggatgt ggtttacgcc gtatcctgaa agcggaatgg aacgggagcg
     4981 catacggggc agggcgaagt tgaacgtgcc gcagtttgca gggcgcaaaa tccgcgtgca
     5041 cggtttgtcg gtattgctcg tcccgcttgt cggcatagac cgcgaaggct accgcttggg
     5101 gcaggcaggc ggctattacg atgcgacgct tgcagcgatg aaataccgtt tgcaggcaaa
     5161 aaccgtgggc gtgggctttg cctgccaact ggtcggcacg ctgccgcgcg aggcgcacga
     5221 cctgccgctg gacggttttg tgtcggaggc ggggatattg tgtttttagc cgttcagacg
     5281 gcatttcggg cggaaatgcc gtctgaaggc ggaatattgg gagaaaagga tgtcggaaaa
     5341 gataatactg ccggtcttgt cattgggcgg cgttcggctc gaaccgttgg atgtgcatca
     5401 tgaaacgggt ttgcgcgagg cggtttgcga tggggaggtt tggaagctgg gggtaacgtc
     5461 cgcgccccat ccggataggg tggcagacta tatcgggaca gcattggcaa cgcgtttggc
     5521 gtttgcggtt gtcgatgaag aggcgggcag ggtggtcggg acaacggcgt attatcattt
     5581 tgaaccgcag attccgcgtt tggatatcgg attcacatgg tatgcggcat cggcacggcg
     5641 gacacggata aacacctgtt gcaaaatcat gctgttggat tacgcatttg atgttttggc
     5701 ttgccgttgc gtgggatggc gcacggatat tctgaacctt gcctcgcaac gtgccatcga
     5761 acggctgggt gcggaaaaag acggcgtgct gcgtatgcat atgctccgta aagacggcag
     5821 tgtgcgcgat acggtcgtgt acagtatgct gagggaagat tggtgcaaaa acagggaaat
     5881 cctgaccggc aggttggcag ggtatggcgt gcaggtgtga ggcagtattt tcaaatttat
     5941 agtggattaa caaaaaccag tacggcgttg cctc
//
LOCUS       DAWHXZ010000130         5971 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000130 DAWHXZ010000000
VERSION     DAWHXZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.130"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1137
                     /locus_tag="WIE35_001485"
     CDS             <1..1137
                     /locus_tag="WIE35_001485"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ4154382.1"
                     /translation="VRTLSDGIKIQALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKL
                     NILGATSGDTGSAAEYALRGKKGVNVFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKG
                     MFDDCQDIVKAVQNDAVFKEKYHIGTVNSINWGRIVAQVVYYFAGYFKATQSNDEQVS
                     FCVPSGNFGNVCAGHIAKQMGLPIRRLIVATNENDVLDEFFKTGAYRPRNSEHTYVTS
                     SPSMDISKASNFERFVFDLMDRDPQEINTLWAEVAAGKGFDLRFALDKVGGKYGFTSG
                     KSTHADRLATIKQVYEQDQELIDPHTADGVKVAREVREEGETVVCLETALAAKFDATI
                     REAVGDVAIPRPAVLKGLETLPQRVQTAPNSADAVKGIIEQTLA"
     gene            1184..1978
                     /locus_tag="WIE35_001486"
     CDS             1184..1978
                     /locus_tag="WIE35_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154383.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSVGIAVPELLAVRK
                     NALMFGNLAGIPLDAQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSEQP
                     AEIQRLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADIPP"
     gene            2166..2942
                     /locus_tag="WIE35_001487"
     CDS             2166..2942
                     /locus_tag="WIE35_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ4154384.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            3018..3124
                     /locus_tag="WIE35_001488"
                     /pseudo
     CDS             3018..3124
                     /locus_tag="WIE35_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3713..>5971)
                     /locus_tag="WIE35_001489"
     CDS             complement(3713..>5971)
                     /locus_tag="WIE35_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEZ4154385.1"
                     /translation="RLYDLPEVQSDFVETHGGTREAVLMLGGITCAACVWLIEQQLLR
                     TDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYDAQKIEAANQKERK
                     QYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLMVLPVVFYCAVPFY
                     QGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFESIAMLLFFLLGGRF
                     MEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLKAGDIVLVKPGETI
                     PVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLIIRTDRTGGGTRLS
                     HIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLYADAHTALWITVAL
                     LVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTDIIFDKTGTLTQGK
                     PAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGSVPDIAIKQRLNRI
                     GEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLGSQSGFQAVFYLTD
                     PLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRAQAMPEDKLEYVKA
                     LQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVLLNEDLRTVAHLLD
                     QARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSSLAVLGNALRLHKR
                     GKMQSEKMPSEQ"
BASE COUNT         1449 a         1586 c         1602 g         1334 t
ORIGIN      
        1 gtccgcacgc tttcagacgg catcaaaatc caagccttgt ccaacggccc gacgctggcg
       61 ttcaaagata tggcaatgca gtttttgggc aatgcgtttg aatatgtttt aaacaaagaa
      121 ggcaaaaaac tcaatatctt gggcgcaacc agcggcgata cgggttcggc tgcggaatat
      181 gccttgcgcg gcaaaaaagg tgtgaatgta tttatgctgt cgcccgatgg caaaatgagc
      241 gcgttccaac gcgcgcaaat gtacagcctg caagacggga atatccacaa tatcgccgtg
      301 aagggaatgt ttgacgactg tcaggatatt gtgaaggcgg tgcagaacga tgccgtgttc
      361 aaggaaaaat accatatcgg tacggtcaac tcgattaact ggggacgcat cgtcgcgcaa
      421 gtggtttatt actttgccgg ctatttcaaa gcgacgcaaa gcaatgacga gcaagtcagc
      481 ttttgcgtgc cgagcggcaa cttcggcaac gtttgcgcgg gacacatcgc caaacaaatg
      541 ggcttgccca tccgccgcct gattgtcgcg accaatgaaa acgatgtgtt ggacgagttt
      601 ttcaaaaccg gcgcataccg cccgcgcaac agcgagcata cttatgttac ctccagcccg
      661 tcgatggaca tttccaaagc gtccaacttc gagcgtttcg tgttcgacct gatggatcgc
      721 gatcctcagg aaatcaatac gctgtgggcg gaagtggctg caggcaaagg ctttgacctg
      781 cgatttgcct tggacaaagt cggtggcaaa tacggcttta cttccggcaa gtccacccac
      841 gccgaccgcc tcgccaccat caaacaggtt tacgagcaag accaagaact catcgacccg
      901 cacactgccg acggcgtaaa agttgcccgc gaagtgcgcg aagaaggtga aacggtggtt
      961 tgtttagaaa ctgcgttggc agcgaaattc gatgcaacca tacgcgaagc cgtcggcgat
     1021 gtcgccattc cgcgccccgc cgtgctgaaa ggtttggaaa cattgccgca gcgcgtgcaa
     1081 accgcgccga atagtgcgga tgcggtaaaa ggcatcatcg aacaaaccct tgcctgatgt
     1141 cccgtgtggg cggcattccg tccgaactta agattgaaag aaaatgacct tacctatgca
     1201 ggaaacccgt ttttctatac tgctggacga actggcggca aaacaggagg cagccatcgc
     1261 tccccacctg cttgccgacg gaaccaaggt atgggtacgc aaagcaggca ggcacaatgc
     1321 gcggtggcgt tacgcgctgc tcggtatggt tgcccgatat ttgaaactgg gggtgttgaa
     1381 gccggttccc agcctcggcg gcgagcctgc cattgcaacc gaatcaaaac gcctgtacga
     1441 attgcgctcg gtggggatag ccgttcccga attgctcgcc gtcaggaaaa atgcccttat
     1501 gttcggcaat ttggcaggca tcccgctcga tgcgcaaatc cgccaagaag ccgaagccgg
     1561 aaaggcggac gcttggcttg ccggtttgga agccattgca cgcgtgcata aaaaacagca
     1621 gttcctcagt caggcgtttg cacgaaatat gatgtgggac ggaaaaaaca tcagctttct
     1681 agattttgaa gacgaccctt ccgaagtcct gaccatagcg caatgccaag cccgcgattg
     1741 gctctgctac atccattcga ccgcgctgat attgaaaaac ggcggactgc tggaagcggc
     1801 tgcggaaaaa tggggcggcg tactgtctga gcagcctgcc gaaatacaaa ggctgattgc
     1861 cgccaccgtc aaaccgatcc tcccgatacg caggctggaa cacccccgct ggggacggga
     1921 cgcgctcagg ctggcagcct cgatttccct gatttccctt gccgatatac cgccataagc
     1981 cgccgcccgc cccgcgatgc cgtctgaagc ccttcagacg gcatataacc aacgcgcatc
     2041 aagcattgtc gcaaccatcc gtttcgttta gaattttgac tttatcccga atcgggcaga
     2101 cattttcact ctgccgcaca tcccgaacaa gcactatcca tttcttttac tgacaggaaa
     2161 cccaaatggc agcattcaat acccaaaaag tattgtccgt acaccactgg acagacgcat
     2221 attttacctt tacctgcacc cgcgacgaat cgttgcgctt tgaaaacgga cagttcgtta
     2281 tggtcggatt gatggtggac ggcaagccgc tgatgcgcgc atacagcgtc gcctccgcca
     2341 actgggaaga acacctcgaa tttttcagca ttaaagtcca agacggtccg ctgaccagcc
     2401 gcctgcaaca cctcaaagtc ggcgacgacg tgttaatcag caaaaaaccg accggaaccc
     2461 tggttgccgg cgacctgaat ccgggcaaac acctttactt gttgagcacc ggtaccggca
     2521 tcgctccttt cttgagcatc accaaagacc ccgaaattta cgagcaattt gaaaaaatca
     2581 tcctcgtaca cggcgtgcgc tacaaaaaag atttggcgta ctacgaccgc tttaccaaag
     2641 aattgcccga acacgaatac ctcggtgact tggttaaaga aaaactgatt tactacccga
     2701 ttgtttcgcg cgaagaattc gaacaccacg gtcgcctaac cgacctgatg gtgagcggca
     2761 aactgtttga agacatcggc ctgcccaaaa tcaacccgca agacgaccgc gccatgctgt
     2821 gcggcagccc cgcgatgctg aaagatacct gcaaagtttt ggacgatttc ggactgaccg
     2881 tctctccgaa aacaggcgta cgcggcgatt acctgattga gcgtgcgttt gtagatcaat
     2941 aagctgtttg aaaatcccgt ctgaacggca ccagcaatca aaaaccccga atcctgccaa
     3001 tcaggattcg gggtttttat agtgaattaa atttaaatca ggacaaggcg gcgagccgca
     3061 gacagtacaa atagtacggc aaggcgagcc aacactgtac cggtttaaat ttaattcact
     3121 atatagtggg taaaaattag aaatgcacat ttccgtcatt ccaacacagg tgggaatccg
     3181 gacttacctg tttcattatc gtttgaaaat aaaagagaaa ccaaacatat agattcccgc
     3241 ttgcgcggga atgacgacgg tgttttgccg tagccaaagc agaaaaacat ccctattgtc
     3301 atttcatcat gatatgacaa taggatgtta aaaattaatt atcattatat gattgatata
     3361 taagaaaaac tgtcccaata agaaatgtta tgcagaaaat aaaatgaaaa ggatggtttt
     3421 cattgaaaat ggttgttctg ccaccgtcat acacagagcc agtatttgga aaaaaatgat
     3481 ccatattgac atcattattc catttgtcgc ataaatccac tttttaattt tttccacatt
     3541 aatataatct cttatttaat aaaatcaaaa taaaaatcaa atgcctaaca attcgatctc
     3601 aaattttgaa cgccaacgtg tttgagcggt aacaatacat actttaagtt tgcaattctt
     3661 catcgtctta tttttgtttt catcaactat acgtctaaag caacgccgtc cgtcattgtt
     3721 cggacggcat tttttcagac tgcattttcc cccgtttgtg aaggcgcagg gcgttgccca
     3781 aaaccgccag cgaactgaag ctcataccca gtgcggctat ccacggttgg acatagccca
     3841 aaacggcaag cggtacggca atgatattgt acgcgcccgc ccatatcagg ttttgccgga
     3901 taatatggcg ggtgcgccgc gcctgatcga gcaggtgggc gacggtacgc aaatcttcgt
     3961 tcaataacac aatgtccgcg ccgtccctcg caatatccgt cccgcccgct gcggcggcgg
     4021 atacgtctgc ctgcgccaaa acgggcgcgt cgttgatgcc gtcgcctatc atcagcactt
     4081 ttttcccttc tttttgcaag gctttgacgt attccagttt gtcctcgggc atggcttggg
     4141 cgcggtagtg cgcgacaccc agggcgcgtg cggtttcggc aacggcggtt tcgcggtcgc
     4201 cgctgaggat gtgcagggtc aggtttttgc ctgccaactg ccgcaccgcc tccgccgcgc
     4261 tgtctttcaa ggggtcggtc aggtagaaca cggcttggaa accgctttga ctgccgaggt
     4321 aaaccgcgct gccgccgcct tctgtttgcg gttctttacc tgaaatttcg gcgacatagg
     4381 atgccctgcc caatgcccaa acctgtgttt cgccgttgac ggtcagttgc gcgcccacgc
     4441 cttcgccgat gcggttgagg cgttgtttaa tagcgatgtc ggggacgctg ccgtctgaaa
     4501 tgcggcagtt gaggatggcg cgggcaaggg gatgttcgga ctgttgttct aaagcctgcg
     4561 ccaccgcgag aacaaaggct tcgtctgtgc ctctcaacaa tgagatacgg cggacggcgg
     4621 gtttgccttg ggtcagcgtg ccggttttgt cgaagatgat gtcggtggtt tgggcgaggg
     4681 tttcgattgc ctgctttccg ccgattaaaa taccttcgcg cgccagcgta ccggtagaag
     4741 ctgccagcgc ggtcggcgtg gcaagcgata aggcgcaggg gcaggtaatg accagcaggg
     4801 cgacggtaat ccacaatgcg gtgtgcgcgt cggcgtacag cgtccagccg atgaagacgg
     4861 ggacggcaag caggagttcg ccgaatatga aagacgaggc gtattgttcc gccaactcgg
     4921 cagtgcgcgg tttttgcgct aaggcgcggt cgagcaggcg gacgatgtgc gacagtcgcg
     4981 tgccaccgcc ggtgcggtcg gtgcgtataa tcagggggct ttgcgtgttg agtgtgccgg
     5041 cggttacttt ttcagacggc attttggcga cgggcaggct ctcgccggtc agcatagatt
     5101 cgttgacggc actgcttcct tccagcaccg tgccgtcaac ggggatggtt tcgcccggtt
     5161 tgaccagcac gatatcgccc gccttcaatt tgacgacagc tgcctcgcag gtttcctgcg
     5221 tatcggggta atcgggcata tgatggcaaa acgcaggaat cagcttcacc agcctctcgg
     5281 cggcatcgcc tgccttacgg cgggcaatgt gttccataaa gcgtccgccc agcaggaaaa
     5341 acagcagcat cgcgatggat tcgaaataca tcccctgccc cgcatttgtc gcaaggctgt
     5401 aaacgccggc gataaaggtc atgatgatgg cgacggtaat cggcgtatcc atgccgacgc
     5461 ggcggttttt caagtcgcgc agcgcgcctt gataaaacgg gacggcgcaa tagaatacga
     5521 cgggcagcac cattaaaaag ccgccccaat ggaggatttg caggaaatcg ggttcgatgt
     5581 cgccgccgta aaggtaggtc ggcagcgcga acatcatcgt ctgcatcatc cccagcccgg
     5641 caacggcgag gcggacgatg tattgtttgc gttctttttg gttggcggct tcgatttttt
     5701 gcgcgtcata gggtgcggcg gtgtagccta tctgcctgat tttcaacaga atgtcggaaa
     5761 ggcggatttt gccgtcgtcc cagacgacgc ggcagcggtg cgtgctgtaa ttgaggtcga
     5821 tgcggacgat gccgtctgta cgcaaaagct gctgttcgat cagccagacg caggcggcgc
     5881 aggtgatgcc gccgagcatt aaaaccgcct cgcgcgtgcc gccgtgggtt tccacaaagt
     5941 cggactggac ttcgggcagg tcgtacaggc g
//
LOCUS       DAWHXZ010000131         5886 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000131 DAWHXZ010000000
VERSION     DAWHXZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.131"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..229
                     /gene="gatB"
                     /locus_tag="WIE35_001490"
     CDS             <1..229
                     /gene="gatB"
                     /locus_tag="WIE35_001490"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEZ4154386.1"
                     /translation="TIAEIIEKHGLQQMTDTGAIEAMVDEVLANNAKAVEQFKSGNEK
                     ALNAIVGQVMKASKGKANPAQVQELIKAKLA"
     gene            321..1331
                     /locus_tag="WIE35_001491"
     CDS             321..1331
                     /locus_tag="WIE35_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ4154387.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMDGHSEQALSEAEKAQGKILMCRTTAQSDISINIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRKNGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQNSSRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(1460..2092)
                     /gene="pdxH"
                     /locus_tag="WIE35_001492"
     CDS             complement(1460..2092)
                     /gene="pdxH"
                     /locus_tag="WIE35_001492"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ4154388.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            2323..3372
                     /locus_tag="WIE35_001493"
     CDS             2323..3372
                     /locus_tag="WIE35_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ4154389.1"
                     /translation="MSKQPTSKRQWRDGVAPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGERRRKKA"
     gene            complement(3441..4982)
                     /locus_tag="WIE35_001494"
     CDS             complement(3441..4982)
                     /locus_tag="WIE35_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ4154390.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVSTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVVETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSVGRQASVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(5593..>5886)
                     /locus_tag="WIE35_001495"
     CDS             complement(5593..>5886)
                     /locus_tag="WIE35_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ4154391.1"
                     /translation="VCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAVSPQHIL
                     ERGFSVVKNTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDCI"
BASE COUNT         1484 a         1745 c         1463 g         1194 t
ORIGIN      
        1 caccattgcc gaaatcattg aaaaacacgg tttgcagcaa atgaccgata ccggcgcgat
       61 tgaagccatg gtggacgaag tgctggcaaa caacgccaaa gccgtggaac agttcaaatc
      121 cggcaacgaa aaagccctga atgcgattgt gggacaagtg atgaaggcca gcaaaggcaa
      181 agccaacccc gcgcaggttc aagagctgat taaagccaaa cttgcctgac aagcaagatg
      241 ccgtctgaaa cccaaatccg acgttcagac ggcattttca cagtctgacc acattaaaac
      301 agcataaaaa acaatattcc atgaaccaca ccgttaccct gcccgaccaa accacctttg
      361 ccgccaacga cggcgaaacc gttttggccg ctgccgcccg acaaaacctc aacctgcccc
      421 attcctgcaa aagcggtgtc tgcggacaat gcaaagccga actggtcagc ggcgatattc
      481 aaatggacgg acactcggaa caggctttat ccgaagcaga aaaagcgcaa ggcaagattt
      541 tgatgtgccg caccaccgcg caaagcgata tcagcatcaa catccccggc tacaaagccg
      601 atgccctacc cgtccgcacc ctgcccgcac gcatcgaaag tattattttc aaacacgatg
      661 tcgccctcct gaaacttgcc ctgcccaaag ccccgccgtt tgccttctac gccgggcaat
      721 acattgattt actgctgccg ggcaacgtca gccgcagcta ctccatcgcc aatccgcccg
      781 accaagaagg cattttggaa ctacacatcc gcaggcgcaa aaacggtgtc tgctcggaaa
      841 tgattttcgg cagcgaaccc aaagtcaaag aaaaaggcat cgtccgcgtt aaaggtccgc
      901 tcggttcgtt taccctgcag gaagatagcg gcaaacccgt catcctgctg gcaaccggca
      961 caggctacgc ccccatccgc agcatcctgc tcgaccttat ccgccaaaac agcagccgcg
     1021 ccgtccattt ctactggggc gcgcgtcatc aagatgattt gtatgccctc gaagaagcac
     1081 aagggttggc atgccgtctg aaaaacgcct gcttcacccc cgtattgtcc cgccccggag
     1141 agggctggca gggaagaaaa gggcacgtac aagacatcgc ggcacaagac caccccgacc
     1201 tgtcggaata cgaagtattt gcctgcggct ctccggccat gaccgaacaa gcaaaaaatc
     1261 tgtttgtgca acagcataag ctgccggaaa acttgttttt ctccgacgca ttcacgccgt
     1321 ccgcatcata attccccggt ataaaaaaga ttcaggcttt ccgttcggaa cacaaaaaac
     1381 ttcccgtccg tattttcccc ataaaaaatg ccgtctgaag cccgattccg gttttcagac
     1441 ggcatatgtt ttttcctgtt caaggcgaca gccgctcgcg tatccagcca ccatccagca
     1501 aacggtattg gatgcggtcg tgcagcctgc tcggcctgcc ctgccaaaac tcaatcaaat
     1561 cgggaatcac aatatagccg ccccaatgcg gcggacgcgg cacgtgcaaa ggatgtttga
     1621 gtccgaccgc cgccgccttt gccaccaata ccgccttgtt cggaatcacc tcgctctgcg
     1681 cacttgccca cgcacccaaa cggctctgat acgggcggct ctcaaaatat tcgtccgaca
     1741 acttctccgc cagcctttca acacgccctt ccacgcgcac ctgacgctcc agctccggcc
     1801 aaaaaaacgt catcgccgca aatggatgag catccagcga acgtccctta cggctgtgat
     1861 aattcgtaaa gaaaacaaaa ccttcagaat taacttcctt cagcagcacc atacggctgt
     1921 tgggtctgcc gcgtccgtcg accgccgcca cattaaccgc cgtcggctcg ttgacctgtg
     1981 cgcgtaccgc ctcgtccaac caccgctcga actgctcgat cggattgtcg gcgcaatcgc
     2041 cttccgacaa ttcccgttta ctgtaatctt cccgaatatt gtgcaaatcc atttaccgct
     2101 cctcttgtca tttgaaagat tctacccccg cacgcaaacc gatttcaacc gttgcacaaa
     2161 ctttgcctcg cttctccaaa cctcaggaca caacgatttc atccgcaaaa ccgccgcata
     2221 aggtacaata tcgaaccgtc cgaccgaggg cggcatttta tcaacccgtc ctgccgcaca
     2281 cgccgcagaa gaaccgcctt atcaggcgag ttaggaaaaa tgatgtccaa acagcccacc
     2341 agcaaacgcc aatggcgcga cggcgtagcc ccgtctgcca agaaaaccgc caaacctttc
     2401 aaaagcaaag cccgtcccaa agacgaaacg gacaaaaccg catcccaacc ttacggacaa
     2461 aaagcttcag acggcatcaa acctcaaaac ctccccaaac agcgcgccgc caaagccaaa
     2521 aaactcgtcg tccgcaatcc caaccaaaaa attatggaac acgcgcgcga tttaaaagaa
     2581 cgccgcagcg acctatcgcg catggaaccc gaacgcctgc aaaaagtgct tgccgcgtcc
     2641 ggcgtcggct cgcgccgcga aatggaagaa tggatcaaca acggctgggt aacggtcaac
     2701 ggcaaaaccg cgcaactggg cgacaaagtc acccccgacg accacgttac cgtcaaaggc
     2761 agcatcatca aactcaaatg ggccgaccgc ctgccgcgca tcatcctata ttacaaacaa
     2821 gaaggcgaaa tcgtttcccg cgacgacccg caaggccgcg tcagcatatt cgaccgcctg
     2881 ccgcaggccg ccagcagccg ctgggtcgcc atcggacgct tggacatcaa caccagcgga
     2941 cttctgattc tcaccacctc cggcgaactc gtccaacgtt tcgcccaccc cagcttcgaa
     3001 gtcgaacgcg aatacgccgt gcgcgtactg ggcgggctga ccggcgaaca aatgcgcgtc
     3061 ctcaccgaag aaggcgtgat gctcgaagac ggcttggcaa aagtcgaacg catccgcgaa
     3121 caaggcggcg aaggcgcgaa caaatggtac aacgtcgtga ttaaagaagg ccgcaaccgc
     3181 gaagtgcgcc gcatttttga aagccaagga ctcaccgtca gccgcctcgt gcgcgtcggc
     3241 ttcggcccca tcggactgcc caaccgcctc aaacgcggac agttctacga actcaacccc
     3301 gccgaagtcg ccaacatcct caaatgggcg gaaatgctgc tgccgggcga acgccgccgc
     3361 aaaaaagcct aaacccgcca aaacacaaaa atgccgtctg aaacatctac tgtttcagac
     3421 ggcattttat tcgggcgttt tcaggagaaa aggtcgagtg ctttgacaaa gaccatcacc
     3481 acgccgtagg cgagcggtat gacggccaat gcccaacgcc accagaccga tatgccgccg
     3541 ccggaaactt tttcgcccac aaggtaagcg tcggatatgg cggtttcgtc atcggggttg
     3601 ccgctgtgtg cggcggtttt gatgtctttt tcgtggtgtt tttcgtgtac ggatttgacg
     3661 gcgaggttgc acaacaaacc gataatcagc aggcacgcca tgatgtacat ggttacgctg
     3721 tatgcctgtg ccgccggtat gccgctgtcg atttggcttt ggcgtatgta attgaccagt
     3781 accgggccga tgacggcggc ggttgaccag gccagcagga tgcgcccgtg aatcgcgccg
     3841 acctgatagg tgccgaacag gtctttcagg taggcgggaa tggcggcaaa tccgccgccg
     3901 tacatggaaa taatcacgca aaagccgatg atgaacaggg ctttgctgcc gccctcgccg
     3961 atggagggaa cggcgaaata cagcagcgaa ccgagtacga agaaaatggt gtaggtgttt
     4021 ttgcgtccga ttttgtcgga aacgctcgac cataaaaagc gtccgcccat gttaaacagg
     4081 ctcaggaggc tgacgaagcc tgccgccgca cctgcgccga ctgacgcctg cctgcctacg
     4141 gaggtttcgg aaaagagttc ctgaatcatc acggatgcct gacccaatac gccaatgccg
     4201 gcggttacgt tcaggcacaa tacccagaac aacagccaaa attgcggcgt tttcatggct
     4261 tgtgacacgt tgacatgatt gctgctgacc agcttgtttt gcgttttcgg cgcggtatag
     4321 ccttcaggtt tccagccgtc ggcaggtacg cggatggtaa acgcgccgaa catcatcagt
     4381 gcgaggtaaa gcaggcccaa tacggcgaag gtttcaacaa ccccgactga agcggcgttt
     4441 gaaaaggcgt tcatcagtga tacggaaagc ggagaggcca gcattgcgcc gccgccgaaa
     4501 cccataatcg ccaaaccggt cgccataccc ggcttgtcgg gaaaccattt catcagtgtg
     4561 gaaaccggcc cgatgtagcc taagcctaag cctacgccac cgatgacgcc gttgcccaaa
     4621 taaagcagga agaggttgtg cgtactcacg ccgaatgcgg atacgaagaa gcccaggctg
     4681 aaacagcagg cggcggcaaa tatggctttg cgcggcccta cccgttccat ccatgtgccg
     4741 aacagggcgg ccgacgcgcc cagcatcgcg agtgcgatac tgaaaatcca acctacggtc
     4801 gtcagcttcc aatctccggc cgccgattcg gttatgccga taagtttggt cagcggcgcg
     4861 ttgaatacgg aataggcgta aatctgcccg atggcaaggt gtaccgccaa tgctgcgggc
     4921 ggtacgagcc aacggttgaa acccggcttg gcaatgcttg cctcacggtc taaaaacttc
     4981 ataacatcct ctttctgtca gttgaaaaat aaaatttcat ttgctcaatg gaaacttatt
     5041 gaaaactata aaaaaatatc ggatcggttt tttatccgcc ccaagatgcc ccgtctgaaa
     5101 catttcgggt gtacggaaag gtttctgttt tttccgacaa attcctgcgg cttttcgctt
     5161 ccggattccc gcttttgcgg gaatgacgaa ttaaagatta tcttaaggtc aagggactgg
     5221 attcccgctt ttgcgggaat gacggcggcg ggttgctgtt ttttatttat aaatttctgt
     5281 aactttggat ttcagccatc ttcaataaat tatcgaaacg tatcaatcgt cattcccgcg
     5341 caggcgggaa tccatccagt tcgttcgatt tcggtttttt aagtttcgag taacttctga
     5401 ctcgtcattc ccgcgtaggc gggaatccgg ttttttgagt ttcagtcatt tctaatacat
     5461 tcccaccact tttcgcttcc ggattcccgc tttttcagga atgacgaatt aaagattatc
     5521 ttaaggtcaa gggactggac tcccgctttt gcgggaatga cggcggaggg ggagcggttt
     5581 ttccgattgg gtttaaatgc aatcgaacaa atcctgctgc ccttgttctt tgcttacgcg
     5641 cacgtcggtt tcgccgtcgg cgaaggtaat gtgcagcttc tgcccctgct tcaaaacatc
     5701 ggcgttgcgg atgacttgtc cgcgtgtgtt tttgacgacg gaaaagccgc gctccagaat
     5761 gtgctgcggc gaaacggctt cgagcaatgc ggcttgggcg gtcaggcttt ggcggcggtg
     5821 ggtaagcagt tggctgaagg cggcgggcaa tgccgtctga aggcggacga tgttgttttt
     5881 gcaaac
//
LOCUS       DAWHXZ010000132         5841 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000132 DAWHXZ010000000
VERSION     DAWHXZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.132"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..997
                     /locus_tag="WIE35_001496"
     CDS             <1..997
                     /locus_tag="WIE35_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ4154392.1"
                     /translation="IPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGSRELSLSGRDL
                     AGFLVDCSAPQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVW
                     QALTHIANSVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRCNIERMDIEWDTD
                     NRFSEVTFLAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKK
                     QLADWRLEGFTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRM
                     DGTQTELRLKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            994..1662
                     /locus_tag="WIE35_001497"
     CDS             994..1662
                     /locus_tag="WIE35_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ4154393.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            1762..2109
                     /locus_tag="WIE35_001498"
     CDS             1762..2109
                     /locus_tag="WIE35_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ4154394.1"
                     /translation="MDKELNPDTGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRVDTA
                     AGGFDYRHEVPVI"
     gene            2123..2566
                     /locus_tag="WIE35_001499"
     CDS             2123..2566
                     /locus_tag="WIE35_001499"
                     /inference="COORDINATES: protein motif:HMM:NF016736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ4154395.1"
                     /translation="MFETPTFEQIRERILRDTKSLWPDADISPDSDHYVHASRLASCA
                     EGQYAHQSWIVRQIFPDTADREYLERHASMRGLRRRNPTTASGTLTVSGIAQSMLSDG
                     LQVRIGQRFYRTTARAVIGSGGTAEIPAIADEPGAAANVATARRN"
     gene            2566..3177
                     /locus_tag="WIE35_001500"
     CDS             2566..3177
                     /locus_tag="WIE35_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ4154396.1"
                     /translation="MAAPAGVATECRLTVQGGTDRESDASLLARLLEIIRRPPAGGNR
                     YDYKNWALSVDGVTSAYVYPLRRGLGTVDIAITSADGVPSEETVRRVQAYIDEMRPVT
                     AKNALVLKPTVTAVPVTVQVKLDGIDLDEAKRRIRTALKEYFDTLIPGDGLTVSQIEA
                     AISNVDGVIDRRLTAPTANRAADTVNRIEWFKAGAINVTEMPS"
     gene            3174..3734
                     /locus_tag="WIE35_001501"
     CDS             3174..3734
                     /locus_tag="WIE35_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ4154397.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            3745..5718
                     /locus_tag="WIE35_001502"
     CDS             3745..5718
                     /locus_tag="WIE35_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ4154398.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGDQTITGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAGGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAANGLAVGTKQEDEIKRHVHKVFSHWVNHPHAA
                     ALGYEDHNERQRSALVSTWTDENLNDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETVNAGALDAGTLAQNLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRHRDSLLEALQD"
BASE COUNT         1417 a         1740 c         1693 g          991 t
ORIGIN      
        1 catacccgat ttaagcggag agagctgcga ggtagtgata gacgggcaaa tcgtgatgac
       61 gggcatcatc ggcagccagc gccacggcaa aagcaagggc agccgcgagt tgagcttgag
      121 cgggcgtgat ttggccggtt ttttggtgga ttgctccgcg ccgcagctca atgtaaaggg
      181 catgacggta ttggatgcag ccaaaaagct ggccgcgccg tggccgcaga ttaaagcggt
      241 ggtgcttaag gccgaaaaca accccgcttt ggacaaaatc gacatcgagc cgggcgaaac
      301 cgtatggcag gcattaaccc atattgccaa ctcggtcggg ctgcatccgt ggctggagcc
      361 ggacggcacg ttggtggtgg gcggtgcgga ttacagcagc ccgccggtgg cgacattgtg
      421 ttggagccgc accgacagcc gctgcaatat cgagcgcatg gacattgagt gggataccga
      481 caaccgcttt tccgaggtta cttttttggc gcaatcgcac ggccgcagcg gcgacagcgc
      541 caaacacgat ttaaagtggg tgtacaaaga cccgacgatg acgctgcacc gccctaaaac
      601 ggtggtggtg tccgatgccg acaatttggc cgcattgcaa aagcaggcta aaaagcagct
      661 ggccgactgg cggctggagg gatttacact cacgataacc gtgggcggcc ataaaacccg
      721 cgacggcgta ttgtggcaac ctggccagcg tgtgcatgtg atcgacgacg agcacggtat
      781 cgatgcggtg ttttttctga tggggcggcg gtttatgcta tcccgcatgg atggcacgca
      841 aaccgagctg cggctcaaag aggacggtat ttggacaccc gacgcttacc ccaaaaaggc
      901 cgaggcggcg cgcaagcgca aaggcaaacg caaaggcgtg agccataagg gcaaaaaagg
      961 cggcaaaaaa caagcagaaa cggcggtgtt tgaatgagtt tgagtaaatt ggcgaaaaaa
     1021 acggcacaaa ctgctaaaaa tatcggcgaa accctgcgcg cggcctttcg gggaaaaatc
     1081 acgctggtgg tgtcgtccga gccgatacag cgcgtgcagt tgagcggctt ggccgacgaa
     1141 accctgcaag accttgaaca tttgcaggaa tacggctttg ccagccatcc gcccgacggc
     1201 agcgaagcgg tagtgatacc gctgggcggc aatacttcgc acggtgtgat tgtgtgcagc
     1261 cagcacggca gctaccgcat caaaaacctt aagcccggcg agacggcgat ttttaatcat
     1321 gagggtgcaa aaatcgtgat taagcaaggc aaaatcattg aggccgattg cgacgtgtac
     1381 cgggttaact gcaaacaata cgaggttaat gcggccacgg atgccaaatt taacgctccg
     1441 ttggtggaga ccagtgcagt gttgacggcg caaggccaaa tcaacggcaa cggcggcatg
     1501 gccgtcgagg gcggcgacgg agccaccttt agcggcgatg ttaaccaaac gggcggcagc
     1561 tttaacaccg acggcgacgt ggtggccggc aatatatcgt tgcgccagca cccgcatacc
     1621 gacagcatcg gcggcaaaac cttaccggcg gaaccggcat agacaagcag acctttggca
     1681 gccttcgggc tgcttttttg tgcgtgcggg attgaagccc gtgtactccg tgaggccgtc
     1741 tgaaaacggc aaaatgccaa catggacaaa gagctaaacc ccgacaccgg cgactatacc
     1801 ggccgcaccg tcgatacgct gcaaaatgcc gtgtatatcc gtttgatgac accgttgggc
     1861 agctggtggg cggataaaac gctcggctcg ctgctgcatt tgttgcagcg cgaaaaagac
     1921 ctgcaacggg tcagcctgtt ggccgagcaa tatgccgatg aggcactgca accgattgtt
     1981 aagagcgggc gtgccgacaa gattaccgtg cgcgcagagc agccgcacga cggccgcctg
     2041 atcctgcata tccgggtgga tacggcggcg ggcgggtttg attaccgcca cgaagtgccc
     2101 gtgatttaaa gaggttttaa acgtgtttga aacgccgaca tttgagcaaa tccgcgagcg
     2161 tatcctgcgc gataccaaaa gcctgtggcc ggatgccgat atcagccccg acagcgacca
     2221 ttatgtgcac gccagccgtt tggccagctg cgccgaaggg caatatgcgc atcaaagctg
     2281 gattgtgcgg cagattttcc ctgataccgc cgaccgcgag tatttggagc ggcatgcctc
     2341 catgcgcggc ttgcgccgcc gcaatcctac cacggccagc ggcacgctga ccgtaagcgg
     2401 tattgcgcaa tccatgcttt cagacggcct gcaagtgcgt atcggccagc gtttttaccg
     2461 cactaccgcc cgcgccgtta tcggcagcgg cggcacggcg gaaataccgg caatcgccga
     2521 cgagccgggc gcggccgcca atgtggcgac ggcgaggcgc aactgatggc cgcccccgcc
     2581 ggtgtggcca ccgaatgccg ccttaccgta caaggcggca ccgaccgaga aagcgatgcc
     2641 tcactgctgg cgcgtctgtt ggaaatcatc cgccgaccgc ccgcaggcgg caaccgttac
     2701 gactataaaa actgggcgtt gagtgttgac ggcgtaacca gcgcatatgt ttatccgctg
     2761 cgccgcggct tgggtacggt ggatattgcc attacctccg ccgacggtgt gccatcggaa
     2821 gaaactgtgc gccgcgtaca ggcttatatc gacgagatgc gcccggtaac ggcaaaaaat
     2881 gcgctggtac tcaagccaac cgtaacggcg gtgcctgtta ccgtgcaagt caagctcgac
     2941 ggcatcgact tggacgaggc caagcgccgc atacggacgg ccctaaaaga atatttcgac
     3001 accctgatcc ccggcgacgg cctgactgtg tcgcaaatcg aggcggctat cagcaatgtg
     3061 gatggtgtga tcgaccgccg tctgactgcg ccgacggcca accgtgccgc cgatacggtt
     3121 aaccgcatcg agtggtttaa agcgggcgcg attaatgtaa cggagatgcc gtcatgagct
     3181 atcaagacat cttgcggggc ctgttgcccc ccgtgtcgta tgcccgcaat gccccgcgtg
     3241 tgcgggcgca ggcagaaata gacggcgcag cgctggatgc ggtggcggaa tcggctcaaa
     3301 gcgttgccga tgccgtcgac ccgcgcagcg ccggccaaat gctggccgat tgggagcgcg
     3361 tattaggttt ggacggtacg ggcaaaaacc gccagcgccg tgtgttggcc gtcatggcca
     3421 agctaaacga aacaggcggc ttgagtattc cttattttgt gcgtttggcc gaggcggcgg
     3481 gctatcaaat ccaaatcgac gaaccgcagc cgttccgcgc cggtgtaaac cgcgccggcg
     3541 accgtcttgc gccgcaggaa atcatgtggg tgtggcacgt taacgtgcgc ggcggcaaca
     3601 accgcattac ccgattccgc gccggtatct cggcggcggg cgacaggctg accgattaca
     3661 gcgatgccgt gatcgagagc ctgttcaacc gcctcaagcc cgcccacacc gctatccgat
     3721 ttacctaccg ataaggacta aaccatgcac cccatcgaaa cccccgacaa gaccttccac
     3781 gacggcgacg gcgtgtccga attgggcacc atcctgcccg cgtggtggct caaccaagtg
     3841 caatccgagc tgctggccgt gctgactgcg gccggtatcc agccggataa gtcgcagcct
     3901 aatcaattac tggcagcact aaataggctg gctgtagtta ccaccggcga ccaaaccatc
     3961 accggccaaa aaacctttac cgcccaaacc caattccaaa gcggcatcca tttatccgcc
     4021 aaccagacga actggaacgg cggccacaaa gcctacatcg gcgcggatgc cgacaacgcc
     4081 cacatcgtct tcggcgacga caccctccgc ctgcacggcg caaacaaccg catttcctac
     4141 aacaaccacg acatcttcca caaagccaac aaaccgcgtt ttgccgaaga catcgaaggt
     4201 aagccgaaca cactgtccgg atacggcatc ggcaatttca aagtcgaaac cttccggggc
     4261 gatttgaaca ccctcaaaac agacggcatc tattccctgc cgacggcggg cggcagctcc
     4321 aacctgcccg ttgaaaacac cgcctgccat atccaagtca tcgccggcac gaaacacggc
     4381 tggtgcaggc agttgggtta tcccgcctac acgtccgacg tgtacgaacg ccaccaaacg
     4441 agcagcgcaa acgacaactg gtccgcatgg aaaaaactca attcggacgg catccccgtc
     4501 ggcgcgatcg tatcctttcc caaagccgtc cgaaaccccg caggctatct caaagccaac
     4561 ggcacgacct ttgcacaaaa caccttcccc gacctctacc gcgccctggg caacagcaac
     4621 cgcctgcccg atttaagccg taccgacatc ggcatcaccg catggtttcc gtccgaccaa
     4681 atcccgaccg gctggctggc gtttgacgac atccgcacgc gcgtaaccga aaccgcttat
     4741 cccgagctgt accgtctgct gaccggaaaa tacggcagca tccaaaacgt cccgcaggcg
     4801 gaagaccgct ttatccgcaa cgccgccaac ggcttggcag tcggaacgaa gcaggaagac
     4861 gaaatcaaac ggcacgtcca caaagtattt tcacactggg taaaccaccc gcacgcggca
     4921 gccctcggtt acgaagacca caacgaaagg cagagaagcg cgctcgtatc gacttggacg
     4981 gacgaaaatt taaacgacaa cggcttttta accccgcgcc cggacagcaa aatggcgaca
     5041 ggcggcgacg aaaaccgccc caaagccctg gttttaaaac tgtgcatcaa agccgccgac
     5101 accttgggcg aagccgtgtt ctggataaag tcccacggcg aaaccgtcaa cgccggcgcg
     5161 ctggacgcgg gcacgctggc gcaaaacctc caagacaaag cagaccgcgc ccacacccac
     5221 accgccgccc aaatccaagg gctggacgaa aaaatcagca ccgccgttgc cgcgcaattc
     5281 acacgccaaa ccatcggcgg cgtggatatt gtcagattcc ccgacggcac aatgatacag
     5341 accggcagct acaggttcac acgaagcggc ggccccatag aaaacgaagt cgtcttcccc
     5401 gtcgcctttg ccgacggcaa cgtcaaatgc ttcgtatccg aacgccattc gggacgcgtt
     5461 accggcgatc gaaggcaaca caactggctg tttatccgcg caaaaaacca cgccgccgcc
     5521 attattacca actggtacga aggcagttgc gactggatgg ccatcggcaa agccgcctcg
     5581 ggaaacgccg ccagctcccc gataggcccc gaaatacctg aaaccaacga agaaccgcaa
     5641 agagagagtg gaagaacatc aaccggcccc cgaaaccgcc gccaccgaga cagcttgctc
     5701 gaggcactgc aagactagcg ggctgtagag atggctgtag agatggctgt agagatggct
     5761 gtagagatgg ctgtagagac gggttgtaga gatgggttgt agagatgggt tgtagagatg
     5821 gctgtagaga tggctgtaga g
//
LOCUS       DAWHXZ010000133         5818 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000133 DAWHXZ010000000
VERSION     DAWHXZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.133"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..1018)
                     /locus_tag="WIE35_001503"
     CDS             complement(<1..1018)
                     /locus_tag="WIE35_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ4154399.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPTRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARF"
     gene            1215..2036
                     /gene="nadE"
                     /locus_tag="WIE35_001504"
     CDS             1215..2036
                     /gene="nadE"
                     /locus_tag="WIE35_001504"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ4154400.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(2106..2579)
                     /locus_tag="WIE35_001505"
     CDS             complement(2106..2579)
                     /locus_tag="WIE35_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ4154401.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAELLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQAPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            2881..3213
                     /gene="trxA"
                     /locus_tag="WIE35_001506"
     CDS             2881..3213
                     /gene="trxA"
                     /locus_tag="WIE35_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ4154402.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(3266..4213)
                     /locus_tag="WIE35_001507"
     CDS             complement(3266..4213)
                     /locus_tag="WIE35_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ4154403.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            4542..>5818
                     /locus_tag="WIE35_001508"
     CDS             4542..>5818
                     /locus_tag="WIE35_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ4154404.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFK"
BASE COUNT         1398 a         1591 c         1517 g         1312 t
ORIGIN      
        1 caaaacgggc ggtgtgggca cggaggtttt gcttcatcga gtaagacagc gtttgcgcca
       61 gcttgccgat tgaagcgcgc tgctcatcaa gtttttggcg cggatgacgg atttgccgcg
      121 caagccagtc gagtttttgg ctggcatcga aatagcgttg ttccaaaacg gttttcagac
      181 ggccttgcgc ctgagcgaga cgatgtagcg attcttggcg gttggggctg accagctccg
      241 ccgcaccggt cggcgtgggc gcgcgcacgt cggcgacaaa atcggcaagc gtaaaatcgg
      301 tttcgtgtcc cacgccactg acgacgggaa ccgtgcaggc ttcgattgcg cgcacgaccg
      361 gttcttcgtt aaacgcccac aagtcttcaa tgctgccgcc gccgcgacag acaatcaaca
      421 cgtcgcattc ggcgcgttgc gaggcggttt taatcgcttg ggcaatttgt aactcgctgc
      481 ctgcgccttg aacaggtgtc ggataaacga taacgggaat ttcgggcgcg cggcgtttca
      541 aggtggtaac gacatcgcgc aatgccgctg ctgccaggct ggtgacgatg ccgatacatt
      601 gcggacgggt aggcaacggc tttttgcgtt ccgccgcaaa cgcgccttcc gcctgcaact
      661 gtgccttcaa ccgctcgtag gcttcgtaaa gctgccccaa acctttgagc cgcacctcat
      721 tcacggtaat ttgaaattcg ccccgcgcct cgtaaatact gatttttcct gatacttcga
      781 tatggtcgcc ttctttcaaa ggcttcgcca aacgcgccgc cgcacctttg aacatcgcgc
      841 aacgcacctg cgcgcggctg tctttgagcg agaaataata atgtccgctg gcggcacggg
      901 tcaggttgga cacttcgccc gcaatccaca ggccggcaag gtggttttcc aaaagacttt
      961 tggcaaatgc gttcaactcg gaaacggaca acacgtcaga atgaaaaaaa tcagacatag
     1021 aatcaatcaa ataataaaaa atatgaatat gttttgaagc ctaaggcggt attagtccgc
     1081 ctaaattgtc aacaatatta taacacgcgc catcttgccg cccgcctttt cccgtatgac
     1141 ttttttaagc ggggaatggg aaaaatattc atcaacccgc cctcaatcta ttcaaattac
     1201 accgccgaca agctatgatg cggatatttt cgacaggagg aaaaatggat acgcaggcag
     1261 ttatcacaca tattgtccga tggttggacg aatacgccgc ccgggcaaat gcaaaagggt
     1321 ttgtcgtggg cgtttccggc ggcatcgatt ccgccgtcgt ctccgcactc gccgcccgca
     1381 ccggccgcac cacgctgctt ctggatatgc cgatacgcca acaccccgac cagcttgagc
     1441 gggcaaggct gcacatccgc aatctgcaac ggcaatatgc caatgtaagc gcacaaacgg
     1501 tcgatctgac cgacaccttc cagacctttg aacaaaccgt cggcgttcat cagacggcat
     1561 ttgacaatca gccgctttcc ctcgccaacg ccagaagccg cctgcgtatg ctgaccctgt
     1621 actactacgg gcagatacac ggactactgg ttacggggac aggtaacaaa attgaagatt
     1681 tcggtgtagg attttttacc aaatacggcg acggcggcgt ggacatcagc ccgattgccg
     1741 acctgaccaa aacccaggtt taccggcttg ccgaagcatt gggcgtggac gaggcgattc
     1801 aaaaagcccc gccgaccgac ggcctgtggg atacggaacg caccgacgaa gaacagatgg
     1861 gcgcaagcta tcccgaactg gagtgggcaa tgggcgtgta cggcacgcgc aaacccgaag
     1921 attttgaagg gcggcagcgc gaagttctgg aaatctatac gcggctgcac cgcgcgatgc
     1981 agcacaaaat caacccgatt cccgtgtgcc gcattccgcc cgaattgttg ggctgaaacg
     2041 tggaaatgcc gtctgaagcg gaaaaccgtg tttcagacgg cattgacata tccggctcct
     2101 atcccttaag aatcgagtac gcgggcaaac aaaatatcgt tttccaaatg aatgtggtcg
     2161 ttcaaatcct ccaccatttc tttggccaaa gcgtaaagcc gcgtccagct tccgcaggct
     2221 ccttcgggcg cttggaaatt atcggtcagc tctttgagcc ttgcgatggc gcggtcgtgt
     2281 tcttcatgtt cgtgcatcat cacgctgatg ggcatcgccg caccccgacc gacaccctga
     2341 ttaatcatcg gaaacagcat cctttcctct ttcatcatat gcatcagcag ttcgttctgc
     2401 atataggcaa gcagctcggc aatttccgcc ggaaaggtat cggcatgaac ttgggccact
     2461 ttttgcgcca gcggcaccaa ttcttcaaat tgtgcacggt gtacattgtg gtagcgttgc
     2521 aggatatgat cgacggttgc accaaaggga gcagtttccc aaacggaaaa atcagtcatg
     2581 gcagggttcc ttttatttat agggtttcgg gtttggtttt gaacattcat actttaagaa
     2641 tctatttaaa cggagcatac accgcccgcg cgcttctgta cagcctcaaa cgtattcctt
     2701 acattttgat aataaaagta attttcagaa ataaaatact gtccgaaccg ttttttagaa
     2761 tttgcaaagg cgattggggc ggtacagaaa aactattatc ccgcccgccc acttgaaatt
     2821 tttatgccca agccctatcc tgcacgctat cgtgccaatc ccaaccgaaa aggaaaaata
     2881 atgagcagcg aattgattgt acacaccagc gatgccgctt ttgaaaaaga cgttttaaat
     2941 gcagatatcc ccgtcctgct ggacttttgg gctccgtggt gcggcccctg caaaatgatt
     3001 gccccgattt tggacgacat tgccgccgaa tttgaaggcc gtctgaaagt ggtcaaaatc
     3061 aacatcgacg acaacgaagc caccccgtcc cgtttcggcg tgcgcggcat tccgaccctg
     3121 atggtgttca aaaacggcga agtcgtcgcc accaaagtcg gcgcattggc aaaaggtcag
     3181 ctgaccgcct ttgtcgaagc ctctatcgcc tgataaagcg caatcgaaaa agccgccgga
     3241 agattccggc ggctttttcg cacccttaag atttgtggcg gatttcccag cacctatgga
     3301 tttttttgtt gcggaaatct tcgggaacgg attgtttgga aatgtctttg acggcgtatt
     3361 gttccgatac caagtcgtct aagacgaagc tgcgcaggtt gttggaaaag tacaaaatgc
     3421 cgtctgaagc gagcagcttc accgcgccgt caatcagctt tttgtggtcg cgctggatgt
     3481 cgaggatgtc ggacattttc ttgctgttgg aaaaactggg cgggtccatc acaatgaggt
     3541 cgaaccgcct gccttcccca tatgccgtct gaagatattg gaacacgtcg gcgcggacga
     3601 ttttatgccg ttccgtatcg atgccgttca attcaaaatt gcgtctcgcc caatcgagat
     3661 aagtgttaga caaatcgaca gtttcgctgg atgccgcgcc gccggtggcg gcatagacag
     3721 tgaagctgcc ggtgtaggaa aacaggttta aaaaacgttt gcccgccgcc gtttcgccga
     3781 cttttttgcg cgtgtttcga tgatccaaaa aaagccccgt atccaaatac ttatcaaggt
     3841 tgacccaaaa cttgcggccg ttttcggtga tgacgaaatc gtcgcccgcc ttgccggttt
     3901 tttcgtattg ctgcaaacct ttttggcgtt cgcggcgttt gaggcggatt tgttcgggcg
     3961 caaaaccggt aacgaaggcg acggcttcca agacttcggc aagccacgct tcgtattctt
     4021 cgggccgcat cagccagccg gtatcgtatt cctgaaggtg gattcgatcg ccgtaaacat
     4081 cggcggcaaa ggggaattgg gggatgtcgc ggtcgtaaat gcgccaggct tcgatgccgt
     4141 tgcgtttcgc ccatttcata aggtgtttga tgtttttgcc caagcggttg gcaaacggtg
     4201 tgatgtcggt cattggtttc aggcggaata aagtggaaaa cggcaatttt actgtaatta
     4261 acgcccgatt gcttgaccgt ttcgggcaaa ccctatacca tccgccgctt atcttgtcat
     4321 acgaagccat cgccttccaa cctaaaccgc ccttacgggc gcgtttcttt tgttgcttgc
     4381 atatttgcca agcctttctg tgcaggttgt cgtcgatgta aaccacaagc aagccgcttg
     4441 cgacaaccct gtaacttcac attccccgta tcgttaccct tccctgcttc aggccgtctg
     4501 aacctttcgg acgcgggcgt tgttgtcttc caaggatagc catgtctatt aaatttgccg
     4561 atttgaacct cgataaaaac atcttgtccg ccgtcagcag cgagggttac gaaagcccga
     4621 cgccgattca ggcgcaggcg attccgtttg ctttggaagg ccgcgacatc atggcttcgg
     4681 cgcaaaccgg ctccggcaaa accgccgcct ttctgttacc gactttgcaa aaactgacca
     4741 aacgcagcga aaaaccgggc aaaggcccgc gtgctttggt gttgaccccg acccgcgaat
     4801 tggcggctca agtcgagaaa aacgcgctgg cgtatgccaa aaatatgcgt tggttccgta
     4861 ccgtcagcat cgtcggcggc gcatcgttcg gctaccaaac ccgcgccctg agcaaaccgg
     4921 tcgatctgat tgtcgccacg ccgggccgtc tgatggacct gatgcaaagc ggcaaagttg
     4981 attttgaacg tttggaagtg ctgattttgg acgaagccga ccgcatgtta gacatgggct
     5041 ttatcgacga catcgaaacc atcgtggaag caacgccgag cgaccgtcag actttgttgt
     5101 tctccgccac ttgggacggc gcggtcggca aactggcgcg caaactgacc aaagaccctg
     5161 aaatcatcga agtcgaacgc gtggacgacc aaggcaaaat cgaagaacaa ctgctgtact
     5221 gcgacgatat gcgtcacaaa aaccgcctgc tcgaccacat cctgcgtgat gccaacatcg
     5281 atcaatgcgt gattttcacg tccaccaaag cgatgaccga agtcatcgcg gatgaattgt
     5341 acgaaaaagg tttcgccgcc aactgcctgc acggcgacat gccgcagggc tggcgcaacc
     5401 gcacgctgat ggatttacgc aaaggccgct gcaaaatttt ggttgccacc gacgttgccg
     5461 cacgcggtat cgacgtaccg accattaccc acgtcatcaa ctacgacctg ccgaaacagg
     5521 cggaagacta cgtccaccgc atcgggcgca ccggccgcgc aggccgcacg ggtattgcga
     5581 ttacgtttgc cgaagtgaac gaatacgtca aagtccacaa aatcgaaaaa tacattaacc
     5641 gcaaactgcc cgaactgacc atcgaaggca tggaaccgac ccgcaaacgc aaatccgcag
     5701 gcggtaagcc aaaaggcaaa ggcggctggg gcgataggaa atccggcggc cggcgcggcg
     5761 atcataaacc gggcaaagaa ggcttcggcg gcaaaacgcg cggcgaaggt ttcaagaa
//
LOCUS       DAWHXZ010000134         5801 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000134 DAWHXZ010000000
VERSION     DAWHXZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.134"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(231..386)
                     /gene="rpmG"
                     /locus_tag="WIE35_001509"
     CDS             complement(231..386)
                     /gene="rpmG"
                     /locus_tag="WIE35_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ4154405.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(418..651)
                     /gene="rpmB"
                     /locus_tag="WIE35_001510"
     CDS             complement(418..651)
                     /gene="rpmB"
                     /locus_tag="WIE35_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ4154406.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(901..2427)
                     /gene="farB"
                     /locus_tag="WIE35_001511"
     CDS             complement(901..2427)
                     /gene="farB"
                     /locus_tag="WIE35_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ4154407.1"
                     /translation="MDYPPLKGAALAWITLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVIFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVTALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPIIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSFVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(2451..3623)
                     /gene="farA"
                     /locus_tag="WIE35_001512"
     CDS             complement(2451..3623)
                     /gene="farA"
                     /locus_tag="WIE35_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ4154408.1"
                     /translation="MATHADETTIQNRQEANSAKVKRKRRLTALTLLFALAAAAAGSA
                     FFLWWQHEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVL
                     AYERAKNELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSA
                     EELAHARAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQ
                     RTQIRAPADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPA
                     ELVSDLYGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDV
                     DRHPLRIGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     gene            complement(3667..4026)
                     /locus_tag="WIE35_001513"
     CDS             complement(3667..4026)
                     /locus_tag="WIE35_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ4154409.1"
                     /translation="MINLELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTDDSDAAD
                     IFSVRICSPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKS
                     LTYLLRFFSWEFEDYQC"
     regulatory      4241..4285
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            4289..4762
                     /gene="queF"
                     /locus_tag="WIE35_001514"
     CDS             4289..4762
                     /gene="queF"
                     /locus_tag="WIE35_001514"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ4154410.1"
                     /translation="MSRNTEELQGISLLGNQKTRYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            4839..5525
                     /locus_tag="WIE35_001515"
     CDS             4839..5525
                     /locus_tag="WIE35_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ4154411.1"
                     /translation="MYAFTDAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYIFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIALVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
BASE COUNT         1460 a         1611 c         1324 g         1406 t
ORIGIN      
        1 gtattattat agtggattaa caaaaatcag gacaaggcga cgaagctgca gacagtacaa
       61 atagtacgga accgattcac ttggtgcttc agcaccttag agaatcgttc tctatccttg
      121 gcagacggtt tgcagcagat tcgcatacgg cagatgtttc aagcggaaaa gaaacacgat
      181 aaaaataaaa aagcctccga acagtcggag gctttgcttt caaactgaaa ttatttcagt
      241 ttggtttctt tgtacactac gtgtttgcgg gcaaccggat cgaatttttt aatttccagt
      301 ttgccgggca tagtgcgttt atttttggta gtggtgtaga agtgaccagt acctgcactg
      361 gattccagtt tgattttatc gcgcattgca gtaatcctta attaaatagt gtttaaatta
      421 agcttcgccg cgagcacgca aatcagccaa tacgacatca atgcctactt tgtcgatggt
      481 acgcagtgca gcgttggaaa cgcgcaggcg aacccagcgg ttttcacttt ctacccaaaa
      541 acgacgtgat tgcaagttgg gcaaaaaacg gcgtttggtt ttgttgttgg cgtgcgatac
      601 gttgttgccg gacatcgggc gtttgccggt cactttgcaa actcgtgcca taatcagtct
      661 ccaaacttct aaaatagtaa aacgcgattt ataccacgaa aaacagtgtt agttcaagtg
      721 ttttaaagaa aaacggctgt tttgcgcgga taatccttcc gattgtcgca tctgtggaac
      781 gtttgcaggt gcgaaacaga tggggcgcga ttatagcgga tttcgggaac tgctgcattc
      841 aaaatgccgt ccgaacatat ttccagtatt ttcagacggc atttcaagtt ttcaaatccc
      901 tcaatgtcca ccgccgccgc cgttgtggaa cggcggtttt gccagccata tgacaggaat
      961 catgatgatg aacagcaggc tgcctgccat aaagatttca ttcgatccga tgatgaagcc
     1021 ctgctgggta atggtattgt tgatgatacc tagggtttga atgtcggaaa tgccttgttg
     1081 ggacaaatgc gccgccgttt cgtgcaatgt tgcggaatag ggcgtgatgt gttcggcaaa
     1141 gcgtgtgtgg tgcaacgctt cgcgccgttc ccacagggta ctgacgaatg atacgccgac
     1201 accgcccatc agcacgcgca agaaattcga caggctgccg gcggcggcaa tctgtccgcc
     1261 cttcatatgc gacagtgtga tggtggtcag cggcaggaag aacatggcaa cgcctgcccc
     1321 ctgccaaaac tgcggccaga tgacgttgcc gatatccatg tcggcataaa aatcggtgcg
     1381 ccaatagaaa gtgaaggcaa aagtcaggaa gctggcggtt acgagcaggc gcatatcgat
     1441 tttattgccg aacctgccga taatggggga caggaaaact gggaggatgc cgacgggcgc
     1501 ggcggcaagc cctgcccacg tggaggtata gcccaggttg gtctgcaaca ctaacggcag
     1561 cagggtcagc gtccccatat acaccataaa acccaatgac gtggcaatga cgccgacggt
     1621 aaaattccga tctttaaaca gcgataaatc gacaatcgga tatttttctc ccaattccca
     1681 aacaataaaa tacgacaagc acaccaatgc ggttacgccc aaggtaatga tttctcccga
     1741 agcgaaccag tcgagttcct tacccctgtc cagcatcatc tgtaacgcgc cgataccgac
     1801 taccatcaat gtaagcccga catagtcggt cggcatttta acggtttccg tttcccgata
     1861 tttcaaatgt ttccatgtaa tccatgccga tatgataccg atagggatat taatgaagaa
     1921 aatccaaccc caatgccagt ttccggaaat ccaaccgccg agtatcggcc cgagaacagg
     1981 ggcgacaacg acggtcattg cccacaacgc cagtgccagc gtccgttttg cgggcggata
     2041 ggatgccatt aacaggcttt gcgacaaggg aatcagcggc ccggcgataa agccctgcaa
     2101 gatgcggaaa ataaccagcg actgaaggtt gggcgcgatg ccgcacagcc acgatgtgat
     2161 gacgaaaccg acagcggcgg cggtaaacaa tttgacctcg ccgatacgtt ttgccaaaaa
     2221 gcccgttaac ggcacggaaa cggcgtttgc cacagcaaaa gaagtaatga cccacgtccc
     2281 ctgagtggtt gccgcaccga ggttgccggc gataacggga acggcgacat tggcgatagt
     2341 cgtatctaaa acttccataa atacggcaag ccccaaagac agcgtaatcc atgccaatgc
     2401 cgcaccctta agcggtggat aatccattgt gtcctccgtt tcagacggca tcagggcgcg
     2461 gattgcccga ggatttcatc gaccgtccga tcgacttccg accagtcggt actttccatt
     2521 tccggcaatg ccgcacccgg cgtttttgaa acaggcgcgc ctgcggcgga agtatccact
     2581 ttaaccgtca tcgacaaacc gatacgcaac ggatgcctgt ccacatcttc gcggttcagc
     2641 acgatacgga cggggacgcg ctgcaccact ttaatccagt tgcccgttgc gttttgcgcc
     2701 ggaatcagcg aaaacgcgct gcccgtacct gccgaaaaac ctgccaccct gccgcgataa
     2761 acaatttgtt tgccgtacaa atcggacacc agctcggcag gctgtccgat tttcatatgc
     2821 cgcaactgcg tctctttaaa attggcatcc acccacacat ccgacagcgg caccaccgcc
     2881 atcagcggcg cgcctgccgc cacctgctgc ccgacctgca ccgaacgctt cgccacctga
     2941 ccgtccgccg gcgcgcggat ttgcgtccgc tgaaggttca accacgcatc tttcaacctg
     3001 ccgattgccg tctgaaccgc cggctgttcg cgcaaagaaa cctgaccgcc caaagccgcg
     3061 cgtgccgaag actcttccgc caaagccgcc ttaactgccg cctgcgcctg agacactgcc
     3121 gcacgggcgt gtgccagctc ttcggcggac accgcgcccg attccgccaa agcagaccgg
     3181 cggcgcaaat catcctgcgc gcgtgccaaa tccgcacggc gcaaggcaac ctgcgccccc
     3241 gcctgcgaag tggcggcatt ttgccggcgg ttttgccgca ccgcctgaac cagctcgttt
     3301 tttgcccgct cgtaagccag cacatcatta tcgtcgtcca ataccgccag cacgtcgcct
     3361 tttttcacgg catccgtatc gtcgtgcaaa accttccgta ccgtaccgcc cttttgcggc
     3421 gtaacctgaa ccacgcgtcc ggcaacataa gcgtcttccg tttcctcttc gtgctgccac
     3481 cataaaaaaa acgccgaccc ggcggctgcg gcggcaagcg cgaacagcag cgtcaatgcc
     3541 gtcaggcggc gtttacgttt gacttttgcg ctattggctt cttgtctgtt ttgaattgtc
     3601 gtttcatctg catgtgttgc cattattcca attccaaata cttctaatac tatgtttttg
     3661 atttaattag cactgataat cttcaaattc ccacgaaaaa aaacgaagta aataagtcaa
     3721 tgacttttcc caagtttctt ttgaacattc tttaagaatt ttctcaattt ccgatttaat
     3781 aacagaatga ttaaattcat tcataatcat catacccgct ccccatttaa ccctttgatt
     3841 ttggaaacaa ttatgcaaaa tccatttagg agagcatatg cgaacagaaa atatatctgc
     3901 agcatcacta tcatcagttc ctatgtctaa atcaattccc acacaaaaat tgtctttgat
     3961 ttcgggaacg aaatcttcaa aggcacaatc gtaaagattg atggctttca attctaggtt
     4021 aatcatttta tattcaaaat aaataaaacc aattaaagat tggttaggat tctataccct
     4081 ggcaataact caatcaagtt aatgatttag aatgaatatt caaataaact gtatcagccc
     4141 ggcctcacag aactatttat atccacacat caccgagtaa agcaattacc catactaacc
     4201 gaagaaaact acacaaatac cgccacttat attacaatcg ccgcccgtgg ttcgaaaacc
     4261 tcccacacta aaaaactaag gaaaccctat gtcccgcaat accgaagagc tgcaaggcat
     4321 ctcgcttttg ggtaatcaga aaacccgata tccgaccggc tacgcgcctg aaattctcga
     4381 agcattcgac aacaaacatc ccgacaacga ctatttcgtc aaattcgtct gcccagagtt
     4441 caccagcctc tgccccatga ccgggcagcc cgacttcgcc accatctaca tccgctacat
     4501 tccgcacatc aaaatggtgg aaagcaaatc cctgaaactc tacctcttca gcttccgcaa
     4561 ccacggcgat tttcatgaag actgcgtcaa catcatcatg aaagacctca ttgccctgat
     4621 ggatccgaaa tacatcgaag tattcggcga gttcacaccg cgcggcggca tcgccattca
     4681 tcctttcgcc aattacggca aagcaggcac cgagtttgaa gcattggcgc gtaaacgcct
     4741 gtttgagcac gacgcacaat aaaccatcct tcccatattt cagacggcat tccttcgata
     4801 cgatatgccg tctgaacgcc tttccgaaag gtttttttat gtacgcattt accgacgcac
     4861 agcaacagaa ggcactcttc tggctggtgc tttttcatat cctcatcatc gccgccagca
     4921 actatctggt gcagttcccc ttccaaattt tcggcatcca caccacttgg ggcgcatttt
     4981 cctttccctt catcttcctt gccaccgacc tgaccgtccg cattttcggt tcgcacttgg
     5041 cacggcggat tatcttttgg gtgatgttcc ccgccctttt gctttcctac atcttttccg
     5101 ttttgttcca caacggcagt tggacaggct tgggcgcgct gtccgaattc aacacctttg
     5161 tcggacgcat cgccttagcc agctttgccg cctatgcgct cggacaaatc cttgatattt
     5221 ttgtattcaa caaattacgc cgtctgaaag cgtggtggat tgccccaacc gcatcaaccg
     5281 tcatcggcaa cgccttggat acgttggtat ttttcgccgt tgccttttac gccagcagcg
     5341 atggatttat ggcggcaaac tggcagggca tcgccttggt cgattacctg ttcaaactca
     5401 ccgtctgcgg tctgtttttc ctgcccgcct acggcgtgat tctgaatctg ctgacgaaaa
     5461 aactgacgac cctgcaaacc aaacaggcac aagaccgccc cgcgccctcg ctgcaaaatc
     5521 cgtaaaacag ccctgccggg agtcgggacg gttgggtttt cttttatttg caaacactgt
     5581 cggaacggat aaatccggat tcccgcccac ttcgtcattc ccgcgaaagc gggaatccgg
     5641 tgcgttgggt ttcagtcatt tccgataaac tcctgccgcg ttgtatttct agattcccgc
     5701 tttcgcggga atgacggcgg atgggtttcc gtgccgtggt gtccggattc ccgcccgtac
     5761 gggaatgacg gcagtgcttc cttggcttaa ttttatttta a
//
LOCUS       DAWHXZ010000135         5792 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000135 DAWHXZ010000000
VERSION     DAWHXZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.135"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(129..890)
                     /locus_tag="WIE35_001516"
     CDS             complement(129..890)
                     /locus_tag="WIE35_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154412.1"
                     /translation="MDVNKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRDHVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMALFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            990..1241
                     /locus_tag="WIE35_001517"
     CDS             990..1241
                     /locus_tag="WIE35_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154413.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1293..2099
                     /locus_tag="WIE35_001518"
     CDS             1293..2099
                     /locus_tag="WIE35_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ4154414.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSEDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALKKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            2406..3929
                     /locus_tag="WIE35_001519"
     CDS             2406..3929
                     /locus_tag="WIE35_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ4154415.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRIDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLDKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTGLLGMIGKSERGAATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVES"
     gene            4027..5439
                     /gene="trkA"
                     /locus_tag="WIE35_001520"
     CDS             4027..5439
                     /gene="trkA"
                     /locus_tag="WIE35_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ4154416.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         1463 a         1719 c         1409 g         1201 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat attaatattt tgcgcccgtt
       61 ccgtgaggct ttcaagtcgg atgagaatgc aaatgccgtc tgaacacggc tttcagacgg
      121 catgacggtc agcgtttgta ttttaactcg tacttgatgt cgttgaggat tttgcggaca
      181 tcgtgttcca acacgtcttc gaccaccgcg cccgcctgct cgtgcagcat ttgctggagc
      241 tgataagtaa agagtgccat ctgcttttgc accgccgttc gaatgatgcc gttgacggta
      301 tcggtcagat gcggacgcag gcgtttgatc agccgttcgg tcagctcctg ttcggacagg
      361 cagaacactt cgcgccggtt gacggctttc gggttcagga tattgatttg gacgggcatc
      421 aacgcctctt ccgcaccgcc ttcgccgttt tccgcaaccc cgtctgccgc cgtacccgat
      481 tctgccgcat cggcgttttc cccgccttca atctgtccgg tttcaaattc ggcactgtct
      541 tttttggcat cgaacaggat tctccgccgc gattcgatgt gtttttccga aaccgacatt
      601 tgcagggaag cctgcgcgtt gagccagttt tcctgaagga caatcatcgg gtcggtttcg
      661 acttcctcgc cgcaatcggc aacggcggca ttgtgttcct cctgccattt tttcagatac
      721 gccttcaaca catggtctcg gctctcatcg tccagtttcg gcacaggcgc gtccgttccg
      781 gtttcagagg ggcgggacag cggcgcgtaa gtcggcactg ccttcatacg gcgcgtctga
      841 cgcaggtttt ccaaacgttt ttcccaattc ggctctttat tcacatccat tttcggcttc
      901 cggttcttaa tctttgcagg cagacaaacc cgcgcccaaa gcgcggtttg atataatggc
      961 gcattttaac agattcgcga ggatacatca tgggcagcat cgaacagcgt ttggaatatc
     1021 tggaagaggc gaacgacgtg ctgcgtatgc agaaccacgt cctgtccacc gcattcaaag
     1081 ccttaatccg cgcccttccc gccgaaaccg ccgaaatcgc ggtcgagtcg attcagcttg
     1141 cttttgagga cgccttggca gaattgagct atgaggacag cccccatacc gatttgttcc
     1201 acgacgttac ttatgcgttt ttccgtgaaa aagaacgtta attttatgtt aaactgattt
     1261 tttaggcttt ttgattaccg aaaggaattt tgatgaatat gaaaaaatgg attgccgccg
     1321 cccttgcctg ttccgcgctc gcgctgtctg cctgcggcgg tcagggtaaa gatgccgccg
     1381 cgcccgccgc aaaccccgac aaagtgtacc gcgtggcttc caacgccgag tttgccccct
     1441 ttgaatcttt agactcgaaa ggcaatgtcg aaggtttcga tgtggatttg atgaacgcga
     1501 tggcgaaggc gggcaatttt aaaatcgaat tcaaacacca gccgtgggac agccttttcc
     1561 ccgccttgaa caacggcgat gcggacgttg tgatgtcggg cgtaaccatt accgacgacc
     1621 gcaaacagtc tatggacttc agcgacccgt attttgaaat cacccaagtc gtcctcgttc
     1681 cgaaaggcaa aaaaatatct tcttccgaag atttgaaaaa catgaacaaa gtcggcgtgg
     1741 taaccggcta cacgggcgat ttctccgtat ccaaactctt gggcaacgac aacccgaaaa
     1801 tcgcgcgctt tgaaaacgtt cccctgatta tcaaagaact ggaaaacggc ggcttagatt
     1861 ccgtagtcag cgacagcgcg gtcatcgcca attatgtgaa aaacaatccg accaaaggaa
     1921 tggacttcgt taccctgccc gacttcacca ccgaacacta cggcatcgcg gtacgcaaag
     1981 gcgacgaagc aaccgtcaaa atgctgaacg atgcgttgaa aaaagtacgc gaaagcggcg
     2041 aatacgacaa aatctacgcc aaatattttg caaaagaaga cggacaggcc gcaaaataag
     2101 cccgcccatc cgaacacaat gccgtctgaa gccctttcag acggcattgt tcatcaatcg
     2161 gcctacaatg aactgcctgc tgatttctcc ctaccgcaaa gcaacaggca aagattacaa
     2221 atatcaaaat ccgagtaaaa cagtatttta ttaaaacaaa ttgataatca agagattaga
     2281 attatgtatt gtctttaccg tacaaacgct ggcactattt caacctgata aaaaacagcc
     2341 ttcaaaaagg ttgcttaaaa caacagcaga cacttaccgc cacaaccttg aaaaggaaca
     2401 caatcatgac cgtcatcaaa caggaagact ttatccaaag catttgcgat gccttccaat
     2461 tcatcagcta ctaccatccc aaagactaca tcgacgcgct ctataaagcg tggcagaaag
     2521 aagaaaaccc cgccgccaaa gacgcgatga cgcagatttt ggtcaacagc cgcatgtgtg
     2581 ccgaaaacaa ccgccccatc tgccaagaca caggtatcgc aaccgtgttt ttgaaagtcg
     2641 gtatggatgt gcagtgggat gcggacatga gcgtggaaga aatggttaac gaaggcgtac
     2701 gccgcgccta cacttgggaa ggcaatacgc tgcgcgcttc cgttctcgcc gacccggccg
     2761 gcaaacgtca aaacaccaaa gacaacacgc ccgccgtcat ccatatgagc atcgtgccgg
     2821 gcgacaaagt cgaagtaacc tgcgcggcaa aaggcggcgg ttctgaaaac aaatccaaac
     2881 tcgccatgct caatccttcc gacaacatcg tcgattgggt attgaaaacc atcccgacga
     2941 tgggcgcggg ctggtgtcct cccggcatct tgggcatcgg catcggcggc acgcccgaaa
     3001 aagccgtgtt gatggcaaaa gaatccttga tgagccacat cgacattcaa gagttgcagg
     3061 aaaaagcagc gtcaggtgca gaattgtcca ccaccgaagc cctgcgcctc gaactctttg
     3121 aaaaagtcaa cgcgctgggc atcggcgcgc aaggcttggg cggtctgacc accgtgttgg
     3181 acgtgaaaat cctcgattac ccgacccatg ccgcttctaa gccgattgcg atgattccca
     3241 actgcgccgc cacccgccac gtagagtttg aattggacgg ctcaggccct gtcgagctga
     3301 ctccaccgcg cgtcgaagac tggcccgatt tgacttacag ccccgacaac ggcaaacgca
     3361 ttgatgttga taagctgacc aaagaagaag tggcgagctg gaaaaccggc gacgtattgc
     3421 tgttgaacgg caaaatcctc accggccgcg atgccgcaca caaacgcctc gtcgatatgc
     3481 tcgacaaagg cgaagaattg cccgtcgatt tcaccaaccg cctgatttac tacgtcggcc
     3541 ctgtcgatcc ggtcggcgat gaagtcgtcg gcccggcagg ccccaccacc gccacccgta
     3601 tggacaaatt cacccgccaa atgctcgaac aaaccggcct cttgggtatg atcggcaaat
     3661 ccgagcgcgg cgcggccacc tgcgaagcca ttgccgacaa caaagccgtg tacctcatgg
     3721 cggtcggcgg cgcggcgtat ctcgtggcaa aagccatcaa atcttccaaa gtcttggcgt
     3781 tccccgaatt gggcatggaa gccatttacg aatttgaagt caaagacatg cccgtaaccg
     3841 tcgccgtgga cagcaaaggc gaatccatcc acgccaccgc cccgccccaa tggcaggcga
     3901 aaatcggcat catccccgtc gaatcttgag gcgccatgcc gtctgaacac aaaatctgct
     3961 ttcagacggc atttccgccc ccggttgcgg tacaatccac catttcatca ctcggcgacc
     4021 cacaccgtga aaatcctcat tttaggcaac ggacaggtag gttctaccgt cgcacaaaac
     4081 cttgccgcca tacccaacaa cgacgtaacc gttatcgaca tcgacgaaaa agcattgcag
     4141 gaaacaggca gccgcctcga tgtccaaacc gttttcggca acggcgcatc ccccttcaca
     4201 ttagaacgcg ccggcgcgga agatgccgac ttgctgctcg cgctctcccg cagcgacgaa
     4261 accaacatcg tcgcctgcaa agttgccgcc gacctgttca acatccccgg ccgcatcgcg
     4321 cgcgtccgtt ccagcgaata cctcgaatac ctcagcccca agctcgaaaa caacgaaaac
     4381 ggcagccttt ccatattcgg cataaccgaa accatcagcc ccgaacagct cgttaccgaa
     4441 cagcttgccg gcctgataga ctgcccgggc gcattgcagg ttttacgttt tgcagacgac
     4501 cgcgtgcgga tggtcatcat acaggcgcgg cgcggcggac tgcttgtcgg acgcagcatt
     4561 gccgacatcg cccaagattt gcccgacggg gccgactgcc aaatctgtgc cgtttaccgc
     4621 aacaaccgcc tcatcgtccc cgcgccgcaa accgtcatca tcgaaggcga cgaaatcctg
     4681 tttgccgccg ccgccgaaaa catcggcgcg gtcatacccg aattgcgccc caaagaaacc
     4741 agcacccgcc gcatcatgat tgccggcggc ggcaacatcg gctaccgtct cgccaagcag
     4801 ctcgaacacg catacaacgt caaaatcatc gaatgtcggc cgcgccgtgc cgaatggata
     4861 gccgaaaacc tcgacaacac cctcgtcctg caaggttcgg caaccgacga aaccctgctc
     4921 gacaacgaat acatcgacga aatcgacgta ttctgcgccc tgaccaacga cgacgaaagc
     4981 aacattatgt ccgccctttt ggcgaaaaac ctcggcgcga agcgcgtcat cggcatcgtc
     5041 aaccgctcaa gctacgtcga tttgctcgaa ggcaacaaaa tcgacatcgt cgtctccccc
     5101 cacctcatca ccatcggctc gatactcgcc cacatccggc gcggcgacat cgttgccgtc
     5161 caccccatcc ggcgcggcac ggcggaagcc atcgaagtcg tcgcacacgg cgacaaaaaa
     5221 acttccgcca tcatcggcag gcgcatcagc ggcatcaaat ggcccgaagg ctgccacatt
     5281 gccgccgtcg tccgcgccgg aaccggcgaa accattatgg gacaccatac cgaaaccgtc
     5341 atccaagacg gcgaccacat catctttttc gtctcgcgcc ggcgcatcct gaacgaactg
     5401 gaaaaactca tccaagtcaa aatgggcttt ttcggataaa ccgccccatt ccggacacat
     5461 tgccgccaag cggtatggaa gcagaaataa tggtaggtgg gcttcagacg gcatccgccc
     5521 tccccgtcat tcccgcgcag gcgggaatct agaccttggg atagcggcaa tattcaaagg
     5581 ttatctgaaa atttagaggt tctagattcc cgttttcacg ggaatgacga agagttgcgg
     5641 gaatctagac cattggaaag cggcaatatt caaaggttag ctgaagcttt agaggttcta
     5701 gattcccgtt tccacgggaa tgacgaaagg tggcgggaat ccagaacgtc gggcaacggc
     5761 aatattcaaa agccgtctga aaatttaaaa gt
//
LOCUS       DAWHXZ010000136         5724 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000136 DAWHXZ010000000
VERSION     DAWHXZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.136"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..717
                     /locus_tag="WIE35_001521"
     CDS             <1..717
                     /locus_tag="WIE35_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ4154417.1"
                     /translation="VLTSATLQSLGNFNLMLRQTGLQWLPETTTLALKSPFDFEKQGE
                     LYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRKQMQDVALRLPGDYL
                     PLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPGTACVQVIIAKLPFA
                     MPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRTEQDYGRVTILDNRI
                     KTQRYGQQLLAGLPPFKRIG"
     gene            753..1367
                     /locus_tag="WIE35_001522"
     CDS             753..1367
                     /locus_tag="WIE35_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ4154418.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGQATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIATAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
     gene            complement(1728..3527)
                     /locus_tag="WIE35_001523"
     CDS             complement(1728..3527)
                     /locus_tag="WIE35_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ4154419.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVMIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPKNTVEIHGRYLYGDQDHLTYFGS"
     gene            complement(3608..4978)
                     /gene="purB"
                     /locus_tag="WIE35_001524"
     CDS             complement(3608..4978)
                     /gene="purB"
                     /locus_tag="WIE35_001524"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ4154420.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGLLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(5050..5682)
                     /locus_tag="WIE35_001525"
     CDS             complement(5050..5682)
                     /locus_tag="WIE35_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ4154421.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
BASE COUNT         1414 a         1588 c         1495 g         1227 t
ORIGIN      
        1 gtgttgacct ccgccaccct gcaatccttg ggcaacttta acctgatgct gcgccaaacc
       61 gggctgcaat ggctgcctga aaccaccacc ctcgccctca aaagcccctt tgactttgaa
      121 aaacagggcg aactctacat cccccccata tacgccagcc ccaaagaccc cgaagcacac
      181 accgccgccg tcatcgagtg gctgcccaag cttatttcgc ccaccgaagc catcggcaca
      241 ctcgtcctgt tttcctcgcg caaacaaatg caggatgtcg ccctgcgcct gcccggagac
      301 tacctgccgc tcctgctcgt acaaggcgaa ttgcccaaag ccgtcctcct gcaaaaacac
      361 caccgggcca tagaagaagg caaagccagc atcatcttcg gactcgacag ctttgccgaa
      421 ggactcgacc tgcccggcac cgcctgcgtg caagtcatca tcgccaaact ccccttcgcc
      481 atgcccgaca accccatcga aaaaacccaa aaccgctgga tagaacagcg cggcggcaac
      541 cccttcatcg aaatcaccgt ccccgaagcc ggcatcaaac tcatccaggc tgtcggccgc
      601 ctcatccgca ccgaacaaga ctacggccgc gtaaccatcc tcgacaaccg catcaaaaca
      661 cagcggtacg gccaacaatt attagccggc ctgccaccgt ttaaaaggat agggtaaacc
      721 taccgccctt cccgcaacag aaaggaaaag tcatgaactt cacccgactg ctcaaccaag
      781 tcttaagcac ggttcaaaaa aaaggcaaca cattctccga cagcccgctc aattcattcg
      841 gcggaggcgc gctggttgcc ggtgtcgcct ccatgctact gaacggtaaa aaccgcaaaa
      901 ccatcaccaa aatcggttcg accgccgctt tgggctacct cgcctaccgg ggctatcaga
      961 tgtggcagca aaacaaaggg caggcaaccg taacacaaag cgatttccaa cctgccggag
     1021 aaactgaaga aacatacagc cgcaccgtat tgcgcaccat gatagccgcc gccgcttcag
     1081 acggcatgat agacgaagcc gaacgccgga ctatcgaaca ggaaagcggc acagaccccg
     1141 aaactgccgc atggctcgcc gccgaatacc gcctgcccgc aagcatcggg gacatcgcca
     1201 ccgccgtcgg caacgatgag gcgttggcgg cggaggccta tctggcggca aggttggtct
     1261 gtgccgattt gtcgcggaaa gaaaccgtct tcctcgcccg cctgtcgcag gctttgaaac
     1321 tggatgacaa tctggtggag agtttggaaa ggcaattggg gttttgatga cacagctggg
     1381 gagggaaaat catcaaaaaa cggttattcc gaccccggcc tcaaaacggc aaaatatagt
     1441 ggattaacaa aaaccagtac ggcgttgcct cgccttagct caaagagaac gattctctaa
     1501 ggtgctgaag caccaagtga atcggttccg tactatttgt actgtctgcg gcttcgtcgc
     1561 cttgtcctga tttttgttaa tccactataa aagccgccgc cccgaacggc aaacagcggc
     1621 ataggctgcc aaaggcaata tccgacaaga aggcaggcta ctgcaatgcg ccgccgtggg
     1681 aagatttaag caggcgttcg tgtttgtgga attcccgccc catataatta agaaccgaaa
     1741 taggtcaggt ggtcttggtc gccgtaaaga tagcgtccgt gtatttcgac cgtgtttttg
     1801 ggcaggtatt tttgtgcgtc cacccaatgc acattgggga tatctttaac caaatcaaaa
     1861 accgcctgat tgctcttgcc gatgtcgccc atagcctgaa tggggcggag atattggttt
     1921 gcggcaaatc ttttcaattt ttcctccctc aggggcgaac ggctgattga tgtgttgttt
     1981 gcaaaaacat agacgggttt gacggcggct atccttttga cggtttccct gaatcgggct
     2041 tggaacccgg gtattaggaa ggattgcgct tcaaatctcg gcacgggctg gccgcccatc
     2101 ctcaaatcat agaattgggc aatgaaaacg gcttcggctt tttcaacttc atcccggtat
     2161 tttcgacata acgggttgtc tgccagcttc tcatctaccc aaaccaaaca ctccgaatcg
     2221 agggacagga ttttggcttt ccacccttcc cggctgccga cataatccag aaacccccgc
     2281 aggtgtccgg cgtgcgagtc gccgagggtc aggacggttt ccggaaaatg attttccgca
     2341 gcaaggggcg cgccgggcaa cgggcggagg tgttcctgtt tcaatatccc ccttgcgtac
     2401 aggttgtaac cgacaagtat cagggacggg gcgagataga ggcagaaaaa tgcctttttg
     2461 aaggtcatct tccgttttct aagcggctgt tcaatcaaat aataactcaa cagggaaaat
     2521 ccggccgtca acgcggcaac cgccgatacg gcaggcagtc cgagctgttt gtcgcctgta
     2581 atgtaatggg cgaaagcaat aaaaatccaa tggtacaggt atagggaata agagattttg
     2641 ccgacaaata cgatggggct tgccgacagg atgcgggtcg gaagtgtccc gtattgcata
     2701 ctccggataa gcagtgccgt cagcaggcag ggaaggagca gggtcattcc cgggataaac
     2761 ggattgtgtt tgtcaatcac gaacaggcag gcaagcaatg cgccgaagca gagtgatgaa
     2821 agcaactgcc gttttccatt tgctgtttgc cgtctgccgt tttgcgtttg cccgtaaacc
     2881 gccagcagcg aacctgccaa cagctcggga aacctcagtg tcgaaaggta ataagtattg
     2941 ggttggttga gaatatcggt ataaaacccg cttggcaaaa acgatgtggc agtcaaaatc
     3001 agaaatagga tgatgctgat gttacgcagc acccgtagcg attttgtttt tttgcagcaa
     3061 aatatcagca aaagaggata caggaggtaa tactgttcct ctactgccaa agaccagata
     3121 tgcagtacgg ggttctcgtc ggcactcaaa tcgaaatacc cctgctgaaa ccccagataa
     3181 atattggaca agaaaaccgc agaaagctcc acggttttcc gcatttggtt gaaatcttcg
     3241 taaaggaaga tttgagaggc aatcaccgaa gccagcgaca cggccgcaat aaaagcagga
     3301 taaatccgct taatcctgcg ggtataaaaa tcccggaaag aaaaagaacc gttctgtatt
     3361 tcagaaagaa tgatgccggt aatgaggaat cctgagatga caaagaaaat gtccaccccc
     3421 aggaatcctc cgggcagcca gcggttattc aggtggaaaa tcatgacgga tagcacggcg
     3481 acggcccgca atccgtcaat ttccggtctg tatcggacag cttgcataaa tatcgccccc
     3541 gtatgtcccg ttatcttaaa aatgccgtct gaacgcgcgt tcagacggca tcggtttcag
     3601 taaacgctca aatccgtttc gccaacgctt cagccttgcc cacatacagc gcgggggtca
     3661 gctcaagcaa tttggctttg gcttcggcgg ggatttccag caatccgata aagcctttca
     3721 gcacttcggg cgtgatgccg cctttgccgc gcgtcaggtc tttcagtttt tcgtaaggat
     3781 tggcgacacc gtaacggcgc atcacggttt gaatcggctc ggcgagcagt tcccaagtgg
     3841 catccaaatc agcggcaagc gcggcagggt tgggttcgag cttgttcaga ccgcgcaggt
     3901 gggcggcgaa acccaacacg gcatagccca cgcccacgcc catattgcgc aatacggtgc
     3961 tgtcggtcag gtcgcgctgc cagcgggaaa tcggcagttt ttcggacaaa aagcccaata
     4021 cggcattggc cataccgagg ttgccctcgg agttttcaaa gtcgatgggg ttgactttgt
     4081 gcggcatggt cgatgagccg acttcgcccg ctttgacttt ttgtttgaag taacccaatg
     4141 aaatataacc ccaaacgtcg cggttaaagt cgatcagaat cgtgttgatg cggctgaggg
     4201 tttggaagaa ttccgccata tagtcgtgcg gctcgatttg gatggtgtag gggttgaagg
     4261 tcagaccgag gctgatttcg acgaagttgc ggcagtgggt ttcccaatct acatcaggat
     4321 aggcgaccat atgggcgttg tagttgccga ccgcgccgtt gattttgccg aggaactctt
     4381 gcgcttgcag gtttttaaac tggcgttgca ggcggtacac gacattggcg gtttctttgc
     4441 ccaaagtggt cggcgtggcg ggctggccgt gggtgcggct catcatcggg acggcggcaa
     4501 ggtcgtgcgc catagcggtc agtttttcga tgatttcggc cagcttcggc agcaaaacag
     4561 cctcacgcgc ttcttgcagc attaaagcgt gggacaggtt gttgatgtct tcgctggtgc
     4621 aggcgaagtg gatgaactca ctcacggcgg cgacttccgg cacttcggca aaacgttttt
     4681 tcagccaata ttcaatggct ttgacatcat gattggtcgt ggcttcgatg gctttgacgg
     4741 cggccgcgtc ttccaatgaa aagttttcaa tcaccgtgtc gatttcggca agcgtttcgg
     4801 cactgaaggg cggcacttcg gcaatcttcg gctcggcggc gagggctttg agccagttta
     4861 attcgacttt gacgcgcgct ttcatcaggc cgtattcgga aaaaatcggg cgcaatgctt
     4921 caacggattg ggcataacgg ccgtctaaag gggaaagcga ggcgatgggg ttgatcattt
     4981 cggcatcctg ttcgggaaag acatcaaaaa ttgttaaatt gtttgcaatt atacatcact
     5041 ctcaggacgc tatgccgtct gaagcccgtg ttccgacaga tacgcaacga tttcgtcttc
     5101 atcgtccgat atggcgaaca aagaggctgc cccttcggaa atcagaccgc gcgccaaaag
     5161 ctgcgcgttt atccactccg ccaagcccga ccaaaacgcc tttccgacca aaacaatcgg
     5221 acgcggcggc actttgcccg tctgcaccaa ggtcaggatt tcaaacaatt cgtccagcgt
     5281 cccgaagccg cccggcatca cgacatatgc ttgggaatag cggaaaaaca ccgccttgcg
     5341 ttcggcaaaa cgggaaaacc gcaaggcgat gtcctgatac ggattcggtt tctgctcgtg
     5401 cggcaaaacg atgttcagcc ccaccgaaac cgacttccct gcaaacgcgc ccttgtttgc
     5461 cgcctccata atccccggcc cgccgcccga aatgacggca atgcccgaat ccgacagccg
     5521 ccgcgccaga cggcaggcga acgcataatc cgcatgattc tgcggcgtgc gcgcgctgcc
     5581 gaagatactg actgccggaa acacgcccgc caatgcttcg tctgcctgcc tgcgttcggc
     5641 atcataacgt gcctgctccg gcacacggtt tgtattctcc attccatcct ccgttcaaaa
     5701 acagcgattg tacaccgtca aaaa
//
LOCUS       DAWHXZ010000137         5657 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000137 DAWHXZ010000000
VERSION     DAWHXZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.137"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            97..813
                     /locus_tag="WIE35_001526"
     CDS             97..813
                     /locus_tag="WIE35_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ4154422.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            962..1864
                     /locus_tag="WIE35_001527"
     CDS             962..1864
                     /locus_tag="WIE35_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ4154423.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGTNLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSENAGMYCLMLLVLLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWVKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIALLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1907..2680
                     /locus_tag="WIE35_001528"
     CDS             1907..2680
                     /locus_tag="WIE35_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ4154424.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EDAGSLKRHHGGASSGGNLPEGLPASRQIQCQNEKNAIARLIAEHIPDESSLFVSIGT
                     TVEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2792..4480
                     /locus_tag="WIE35_001529"
     CDS             2792..4480
                     /locus_tag="WIE35_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ4154425.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVLELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4573..5529
                     /gene="gshB"
                     /locus_tag="WIE35_001530"
     CDS             4573..5529
                     /gene="gshB"
                     /locus_tag="WIE35_001530"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ4154426.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSSGLSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMQLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELSERDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1311 a         1546 c         1560 g         1240 t
ORIGIN      
        1 atttgtgtaa tactttgatt tccccgacac gatttgaagc acagccgcct taaaacatct
       61 taaaaaattt gaaagcgtaa aaatacggaa aacatcatga actttgaaaa cgacgacatt
      121 atccatgcgc cgaccacgtc ttccttgatt ctcgaagagc ggcacgattc ggagctgttc
      181 cgtgtttacg ccctgatttt ggacggcatt accgatcagg tgctgctgcc cggcaaaaag
      241 ctgaccgaat ccgaactttg ccgccagatg gtgtgttcgc gcaacaccgt ccgcggcgcg
      301 ttgtcgcttt tggcgcacga caagattgtc gatttgcaac ccaacagggg cgcgttcgtc
      361 cacgttcccg atttgaaaga aatgcaggat gtgttcaatg cgcgcatcga aatggagacg
      421 atgattttga atatcctcgc agatttgccg gatttggaaa cgcgcctcaa gccgctttat
      481 gcgatgatac ggcgcgaaga agaggcctcc ggcaggggag accgcgttgg ctggaaccgc
      541 ctgtccaatg ccttccacgt cgaactggcg cgcctggtgg gcaacgatgt gctgttcgac
      601 attatgaaca cgctgtgcgc gcggtcttcc ctgattgtcg ccgtggcggg cgtgcatcgc
      661 gaggaaaaac acgccatcaa tacgcatacg cattccgaac accgcgaaat cctcgacctg
      721 ctgctggcgg gcaggcgcaa ccgcgtggtc aaaatcctgc gccgccattt gggcaactgt
      781 atggagcgtt tggaaaagac tttggaagat tgaatgccgg agcggataag ccgcctgaac
      841 cttcaggcgg ttttttaatg gcgaacctga tgccgtctga aatatggatg cgggtatctg
      901 caattttcag acggcaattt ttaaaccgca tatatcatgc ggcaataaag gaggtcagga
      961 gatggacagc ctgatgacgt tgctttcggt attgataccg atgtttgccg gattttttat
     1021 ccgtgtgccc aagccttacc tgcccgcttt ggataaggtg ctatcggtct tggtgtatgc
     1081 tgtgctgctg ctgatcggcg tctcgttgtc gcgcgtggag gatttgggtt cgcggttgga
     1141 cgatatggcg ttgacggttc tgtggctgtt tgtttgtacg gtcgggacga acctgcttgc
     1201 tttggcagtg ttgggaaagt tattcccgtg gcggataaag gggaaaggga agggcgtttc
     1261 ggtcggtgtg tcgggcagtg tggggcagct cggatgcgtg ctgctcggat ttgcatccgg
     1321 caaactgatg cgcgatattt ggatgccgtc tgaaaacgcg ggtatgtatt gtctgatgct
     1381 gctggtgctc ctcatcggcg tacagctcaa aagcagcggc gtatcgttgc ggcaggtttt
     1441 ggtcaaccgc aggggtattc ggttgtcggt ctggtttatg ctttcatctc tttcaggcgg
     1501 gctgctgttt gccgcatcgg cagacggtgt gtcgtgggtg aaaggtttgg cgatggcttc
     1561 cggcttcggt tggtattccc tctcgggttt ggtgatgacc gaggcttacg gcgcggtatg
     1621 gggcagtatc gcgcttttga acgatttggc acgagagctg ttcgcgctgg catttattcc
     1681 gctgctgatg aagcgttttc ccgatgcggc agtgggggtc ggcggcgcga ccagtatgga
     1741 tttcacattg cccgtgattc ggggtgcggg cggcttggaa gccgtaccgg tagcggtcag
     1801 cttcggcgtg gtggtcaata tcgccgctcc gtttctgatg gtggtgtttt ccgctttggg
     1861 ctgaacgcgg taaaatcggc atcccgatgc aaggaggcag aaaacgatga aaccgaaaat
     1921 ccaaaggcac ggagagattt taagccttgt ccgccggcat cagtttatgt cggtggacga
     1981 gcttgccgcc gcattggacg ttaccccgca gacgatacgc cgcgacatcc gcgaattgga
     2041 ggatgctggc agcctgaaac gccaccacgg cggcgcatcc tcgggcggaa acttgccgga
     2101 ggggctgcct gccagccgcc aaattcaatg tcaaaacgaa aaaaacgcca ttgcccggct
     2161 gattgcggaa catattcccg acgagtcatc gctgtttgtc agtatcggca cgacggtgga
     2221 agccgtggca tcggagctgg tgagacggcg cggcagcctg cggattatta ccaacaatat
     2281 ccacgtcgcc tccatcgttt cggcgcggac ggattacacg gtcatcatca cttccggagt
     2341 cgtccgccct ttggacggcg gcattaccgg cgtggcaacc gtcgatttta tcaaccagtt
     2401 caaagtcgat tatgccgtga tgagtacgca cggcgtggaa agcgacggtt cgcttttgga
     2461 cgacgactac aaagaagtca gcgtgatgca ggcgatgatt gccaacgcgc gcgtccgctt
     2521 cctcggcgtg gaccacagta aattccgcag caacgcgctg gtcaggctcg gcgacattac
     2581 ggcgtttgac aaagtattta ccgaccggct gcccgatacc gcgatgcaga agatgctgaa
     2641 agaggcgggg gtggaatgcc tgattgccga tgccgtctga acgctatgtc aaagcgcgca
     2701 agtcgggtac aataaacaca tcatcaaacc gcttcagacg gcataaggaa ccgccccaat
     2761 gccgtctgaa gccatctgtt caaagaaaac tatgctcaat aaagaccaat ttgcggacaa
     2821 ccattttatc cgcaccttca tcgaagaaga cctcaaaagc ggcaaacacg aaaccatcca
     2881 aacccgtttt ccgccggagc ccaacggcta cctgcacatc ggacacgcca aatccatctg
     2941 cctgaacttc ggtttggcgt atatttacga cggcttgtgc aacctgcgtt ttgacgatac
     3001 caaccccgaa aaagaaaacg acgaatacgt caacgccatc aaagaagacg ttgagtggct
     3061 cggtttccat tgggcgggcg agccgcgttt cgcttccaac tatttcgacc agctttatga
     3121 ctacgccgtc ggtttggtta aagacggcaa agcgtatgtc gatgatttga cgccagaaga
     3181 aatgcgcgaa taccgcggta cgctgaccga agcaggcaaa aacagccctt atcgcgaccg
     3241 cagtatcgaa gaaaacctcg acctgttcac gcgcatgaaa aacggtgagt tccccgacgg
     3301 cagcaaaacc ctgcgcctga aaatcgacat ggcatccggc aacatcaata tgcgcgatcc
     3361 ggtcatctac cgcatccgcc gcgcccatca ccacaacacc ggcgacaaat ggtgcatcta
     3421 tccgatgtac gactacacgc attgtatttc cgatgccatc gaaggcatca cgcattcctt
     3481 gtgtacgctt gagtttgaag cccatcgtcc gctgtatgac tgggtgttgg acaacatccc
     3541 cgcgccgcac gccacacgtc cgcgccaata cgagttttca cgcttggagc ttttgtacac
     3601 cattacctcc aaacggaaat tgaatcagtt ggttgtggaa aaacacgttt ccggctggga
     3661 cgatccgcgt atgccgacca tttccggtat gcgccgacgc ggctacacgc ccgaagggct
     3721 gcgcctgttt gccaaacgcg ccggtatttc caaatctgaa aacatcgtcg atatgagcgt
     3781 gttggaaggc gcgattcgcg aagagttgga aaattccgcc ccacgcctga tggcggtgtt
     3841 gaacccgctc aaagtaaccc tgaccaactt tgaaaccggc agaacccaaa gccgccgtgc
     3901 cgcgttccat ccgaaccacg aagaaatggg cgatcgcgaa gtacctgttt cacaaaccat
     3961 ttacatcgaa gccgacgact ttgccgaaaa tccgcccaaa ggcttcaaac gcctgattcc
     4021 cggcggcgaa gtacgcttgc gccacggcta tgtcatcaag tgcgatgaag tcgtcaaaga
     4081 cgaagcaggc aatgtgcttg aactcaaatg cagcatcgac cacgacacct tgggcaaaaa
     4141 tccagaaggc cgtaaagtta aaggcgtgat tcattgggtt tccgccgaac acgccgccga
     4201 aatcaaagtc cgtctgtacg accgcctctt taccgtcgag cgtcccgatg ccgtgcgcgg
     4261 cgaagacggc gaatacctgc cgttcaccga tttcctcaat ccggaatccg tcaaggaaat
     4321 caccgcctac gccgaacctg ccgccaaaga tttgccggca gaaagccgtt ggcagttcga
     4381 gcgtatcggc tacttcgtaa ccgaccgtaa agaccacggc aaagacacgc cggtgtttaa
     4441 ccgcacggtc acgctgaaag attcttggca gcctaagtaa aaccccatcc ttgccgtctg
     4501 aatattgttc ggacggcatt tcccctttgc ccgcgaaatg cggcacattc ggcacaccgg
     4561 aaaggaaata tgatgaaagt cctctttatc gccgacccga tggcaagttt caaaacctac
     4621 aaagacacca cctacgccat gatgcgcgag atggcaaaac gcggctggcg gctgtttcat
     4681 accttgagca gcggattgtc ggttaaaaac ggtctggtgg ttgctgatgc gtctgctttt
     4741 gagtttctcg gcgcacaaga cgaccacgac aaaaactggt ttgccgccgc cgacaaagtt
     4801 cagacggcat tgaaagcatt tgatgccgtg attatgcgta ccgatccgcc gttcgatatg
     4861 caatacctct acgccacaca gcttctgacg ctggcggagc agcagggcgc gaaggtcttt
     4921 aacagcggac agtcgatgcg tgacttcaat gaaaaactgg cgattttgaa cttcagccgc
     4981 tttaccgcgc ccacgctggt aacgacccgt tccgccgatg tccgcgcatt tttgaaagaa
     5041 cacggcgaca tcatcattaa accgctcgac ggcatgggcg gtatgggcat cttccgcctg
     5101 accgaaaaag accccaacat cggcagcatc ctcgaaaccc tgatgcagct tgattcccgc
     5161 accattatgg cgcaacgcta cattcccgaa atcgtacacg gtgacaaacg catcctgatt
     5221 atcggcggcg aagtcgtccc ctatgctttg gcgcgtattc ctcaaaacgg cgaaacacgc
     5281 ggcaaccttg cggcaggcgg gcgcggcgtg gcgcaggaat tgagcgaacg cgaccgcgaa
     5341 attgccgaaa cgctcgcccc cgaactcaaa cggcgcggca tcctgctggc cggtttggac
     5401 gttatcggca gcaacctgac cgaagtcaac gtaaccagcc cgaccggatt ccaagaaatt
     5461 atgaaacaaa aaggcttcga tgtggcggca atgtttgccg atgccgttgc cgcgtggtcg
     5521 gtacgttaaa ccgatgccgt ctgaaaggct tttgcttcac aaccgcctgg tttgatcggg
     5581 caggcagggt tttccctcag tcggcagaga tacgcttaat accgtacgga caccccgccg
     5641 ccccgttttc agacggc
//
LOCUS       DAWHXZ010000138         5603 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000138 DAWHXZ010000000
VERSION     DAWHXZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.138"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(65..418)
                     /locus_tag="WIE35_001531"
     CDS             complement(65..418)
                     /locus_tag="WIE35_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ4154427.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            complement(786..1124)
                     /locus_tag="WIE35_001532"
     CDS             complement(786..1124)
                     /locus_tag="WIE35_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154428.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            complement(1260..2120)
                     /locus_tag="WIE35_001533"
     CDS             complement(1260..2120)
                     /locus_tag="WIE35_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ4154429.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            2278..2847
                     /locus_tag="WIE35_001534"
     CDS             2278..2847
                     /locus_tag="WIE35_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ4154430.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            complement(2909..3772)
                     /locus_tag="WIE35_001535"
     CDS             complement(2909..3772)
                     /locus_tag="WIE35_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154431.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKE
                     IVFGTTVGDFGDMVKEQIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPVAWNEGAAK"
     gene            complement(3931..4617)
                     /locus_tag="WIE35_001536"
     CDS             complement(3931..4617)
                     /locus_tag="WIE35_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ4154432.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKAVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIIRKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            complement(4619..5356)
                     /locus_tag="WIE35_001537"
     CDS             complement(4619..5356)
                     /locus_tag="WIE35_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ4154433.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKTRVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
BASE COUNT         1234 a         1361 c         1625 g         1383 t
ORIGIN      
        1 aacatccgac aaactggtca agctctgcaa gtggtgcttg atgtagtcgg cagcggtaat
       61 ggtttcacct gccataatct ttcactctca acaatactaa aaaaaccaaa tggctgacac
      121 cgagcagccc catcagaaac ggggcgaaca ccaacgattg atgccataca gcaaatacgg
      181 cgagcatgga caacagcgac agcactactt ttaaaatctc tccgaagacg aacatcctgc
      241 tttgcaggaa ggggtttccc ctgaaaagtt ttaaaagtaa aactgcaaca aacgtgggaa
      301 gcaggtagga caaaccgcca ccgaccgccg aaaggaatcc ggcaaaaccc catacagcaa
      361 aggcaactgc ggcgcatatg gacaatacgg cggattgtag gatgataatc tgcttcataa
      421 agggaatgtt tccgcctcgg atttggggcg cggctaatat aatttagaag ccttattacg
      481 tcaagcgaca gttaatcttt gtgaaacaac gtatcccaat ccgccgcacc cgccgcctga
      541 ataacggcga caggtgtcat tctaacacac attacatata attacaggat attaaggagt
      601 ttgtccgcaa tttctttaca cttttaatgt tcttacgtga tttgtttgct ttacgtggaa
      661 ataataaaaa atcaacgcga aattgtagca gtttatcggt cggattgtcg gcagtttggg
      721 gaatttgctc aataaataaa aggtcgtctg aaaatatttt cagacgacct tttccgaata
      781 aaggattagc aacggcctgc cgctttaagc aaagcattgc attgactttt gcctttgtgc
      841 gttccgcctc ccaaacaaat tgcatcggaa gtggtaacgc cgattgtgct gattacactg
      901 gtaacatagc attggctcac gcgcttaccc acagttgcgg taaagttgat gcgtatgcct
      961 tcattgttgc ggttgctgat ttttacggca tttgggctga cgcccaaggc aaacgcggca
     1021 cgttcctgaa gtttctggtc ggaaacggtt acattattga ttgagccgca acctgctaat
     1081 gccaacgcaa cgaacgcagc cgaaacgatg atgcgtgtgt tcataatttc ctcgaaaatt
     1141 aaaaatgaaa acaggaaaac gattcttacg tgaagcagaa aaaatgtcaa tagaattata
     1201 tttcccactt aaaatctgga aagctattct ctatatttca gacggtatat cccgcaaaat
     1261 taaggtcggt aatctatgcc caattgcttc aagatatggt cgaaggtttc gggcgtgtcg
     1321 aaatgcagga tgattctgcc ttttttatgg ttggcggttt tgatttcggc gttgacaccc
     1381 agtttttcag tcagcaaatc attcaggcgg ccgatgtcgg cggcggcagt ttttttgggc
     1441 tcgggacgtt tgttttgaag ggcggcttgg ctgcggcgtt cgacttcgcg caccgaccag
     1501 ccgtttttga cagccttttg cgccaattcg agctgttcga cgacgggcag ggtcagcagt
     1561 gcgcgggcgt gccccatttc gaggcggcgt tggtaaagca tttcctgcac gggttcgggc
     1621 aggcttaaaa ggcgcaggct gttggaaatc gcgcttcggc ttttaccgac ggcttgggcg
     1681 atggtttcgt gggtcagccc gaactcgtcg gcaaggcgtt tcaagccttg tgcttcttcg
     1741 atggggttga ggttttcgcg ctggaggttt tcgatcaaac ccattgccaa cgcggtttcg
     1801 tcgctgatgg ttttgataac ggcggggatt tcggtcaggc cggcaatctg tgcggcacgc
     1861 caacgccgct cccccgcaat cagttcgtat cgggacagtc cgtgttcgcg cacgatgacg
     1921 ggctgtatca cgccttgcgc cttaatcgaa tctgccagct cctgcaaggc ttcgtcatcg
     1981 atttgaacac gcgcctgata gcggccgggc cggatatctt taaccgcaac cgtggtcaat
     2041 cggtcgccgc tgctgttgtc tgcgccgttg gcgagcagcg aatccaaacc gcgccccaat
     2101 ccgcctttta cttttgccat accgccctcc cgtgcctatt cagataggat gttaaatcgg
     2161 atattttatc ggatattggg tgttgccgac aatttgtatc cgtatttatc ggatttctgt
     2221 tttttcacta taatagccgg tttgccgttg caggcggttt tatgggaaag gcggatgatg
     2281 gtacggcgtt tgataatcgg catcagtggg gcgagcggtt tccaatacgg cgtgaaggct
     2341 ttggaacttt tacgcgcgca agatatcgaa acgcaccttg tggtatcgaa aggtgcggag
     2401 atggcgcgcg cttcggaaac ggcttatgcg agagacgagg tatatgccct cgcggacttc
     2461 gtgcatccga tcggcaatat cggggcgtgc attgccagcg gtacgtttaa aacggacggg
     2521 atgctggtcg ccccctgttc gatgcggacg cttgcctcgg tcgcgcacgg cttcggcgac
     2581 aacctcttga cgcgtgcggc ggatgtggtt ttgaaggaaa ggcggcggct ggtgctgatg
     2641 gtgcgcgaaa cgccgctgaa ccttgcccat ttggacaata tgaagcgggt aacggaaatg
     2701 ggcggcgtgg tgtttccccc tgttcctgcg atgtaccgca aaccgcagac ggcggacgac
     2761 atagtggcgc acagtgttgc acacgctttg tcgctgttcg gaatcgatac gccggattcg
     2821 gcggaatggc agggaatggc ggattaaagg acaaaaatgc cgtctgaaca cggatacggt
     2881 tcagacggca tcattttata cgactgcctt atttggctgc gccttcattc catgcgacag
     2941 gggatttgta gccctcgaag cgtttgtgcg cgtaggcttt gaacgcgtcg gagttatagg
     3001 cctcggttac gtctttaagc cattggctgt ctttgtcggc ggttttgacg gcagaccagt
     3061 tgacataggc aaagctcggt tcttggaaca gggcttcggt cagcttcatg ccgctgctta
     3121 tggcgtagtt gccgttgacg acggcaaaat ccacgtcggc gcggctacgc ggcagttgcg
     3181 cggcttcaag ctcgacgatt ttgatgtttt tcaggttttc ggcaatgtcc gctttggatg
     3241 cggtcagcgg attgatgccg tctttgagtt tgatccaacc cagttcgtcg agcatcacca
     3301 agacgcgggc gaagttggac gggtcgttgg gcgcggatac ggtgctgccg tctttgactt
     3361 cttccagcga tttcagcttg cccgggtaca gtcccaaagg cgcggtcggc acttggaaga
     3421 cttcggtgat gtccagattg tgttcttttt tgaagtcgtc aagatagggt ttgtgttgga
     3481 agacgttgat gtccaactcg ccctcagcca atgccagatt cgggcgcaca tagtcggtaa
     3541 actcgaccag tttgacggtg tagccttttt tctccagctc gggttggatt tgttctttga
     3601 ccatatcgcc gaagtcgccg acggtcgtgc cgaagacgat ttcttttttc gccgcgccgt
     3661 tgtcggcggc ggcagaagcg gatgcggcgg gcgcgctatc tttttgaccg ccgcaggcgg
     3721 cgaggatgag cgcgagtgcg gcggcggaaa gggttttgaa gaaggttttc atattttctc
     3781 ctgatgttgt ggcagtttca aacaaaaatg acgggcaggg agtcctgccg tccggattcg
     3841 gcgttcagac ggcatttgcc gcgaacaggg ggattttata gcatttttcg gatagcggtg
     3901 ggggttttgg cgttcagacg gcatttgggt tcaacgtttg tcgagtttcc gcgccaacgc
     3961 gttgccggtg ctttgaatca ggatgaccag cagcacgagg agggcgacga tgaagacgat
     4021 gacttcggtt tggtagcggt agtagccgta gcggatggcg aggtcgccca agccgccgcc
     4081 gcctatcatc cctgccgccg cgctgtatga caaaagcccg atggcaagca cggtaatgct
     4141 ggaaaccatg cccgcgcgcg cttcgtttaa gaggactttg cggatgatgg caaccggcgg
     4201 cgcacccata gcggcggcgg cttcaatcac gcctttgggg acttcgcgca ggttttgttc
     4261 caccaaacgg gcaaaataaa acaatcccga cacgctcaac accagcgagg cggcaaccgg
     4321 accgatggtg ctgccgacga tggcgcgtgt ggcgggtatc atcgcaatca tcaaaatgac
     4381 gaaggggaag gcgcgcatga ggttgacgag gttgtcgagc aggaagttca ccgccttgtt
     4441 gtaatgcagt tggcggctgg aagttacgaa gagcaacacg cccagcaacg tgccgaaaac
     4501 gacggcgaat gtggtggaca agccgaccat cacgaaggtt tcgcccaatg cgcggacgat
     4561 ttcgcctttc ataccgatga tgttggaaac ggcttgttgg aatgttaagt ctgccatatc
     4621 agtcctcccg aatcagttcg cgcccgatgt cggatcgggc gtggatttgg ttgccgcgta
     4681 cttcgacgat ttcgacgact ttgcctttgt ccaacagggc ggcgcggtcg cacagtcgtc
     4741 tgatgacgct catttcgtgg gttacgatga cgatggttac gttgaatcgt ttgttgatgt
     4801 cttccaaaca ttccaagacg ctgcgcgtgg tggcggggtc gagggcggaa gtgggttcgt
     4861 cggcgaggat gacttggggt ttgggcgcga gtgcgcgggc gatgccgaca cgctgtttct
     4921 gcccgccgga aagctgggcg ggatagtggc cggcgcgttc ggtcagtccg acgatttcga
     4981 ggcattcggc aacgcgcgtt ttgatttttt cagacggcca tccggcgatt tccaatggga
     5041 aggcgacgtt gccggcgacg gtgcggttgt tcaacaggtt gaactgttga aataccatgc
     5101 cgatattctg ccgcgcctga cgcaatgcgg cggcatcaag cgcggtcagc tcttgtccgc
     5161 agacgttgac cttgccgctg tcggggcgtt ccaacaggtt aatcaggcgc aacagggtgg
     5221 atttgcctgc acccgaataa cccatcagcc cgaagatttc gccgtcgcgg atttcgaggc
     5281 tggtcggctc gacggcggca aaacgggtct tgtcgcgcgt ttggtaatgc ttggaaacct
     5341 tgtccaaaat aatcattgtc ttttccatac aacaaagccc gatgtcggac acaacgggcg
     5401 cggaagataa agctgaaatt gtcggaacgc tttagctgtt atgcccgcaa gctgtgtcaa
     5461 atcggcaggt taattttcgt aggatattat cgggaaagca tttttttgtc aataaagcag
     5521 gaagcgggca accatttcgg acaatgccgt ctgaaacggg caaaggcagc ggttcgcacc
     5581 aaaacggcaa ataattgaaa aac
//
LOCUS       DAWHXZ010000139         5516 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000139 DAWHXZ010000000
VERSION     DAWHXZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.139"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(94..990)
                     /gene="argB"
                     /locus_tag="WIE35_001538"
     CDS             complement(94..990)
                     /gene="argB"
                     /locus_tag="WIE35_001538"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ4154434.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1160..2290
                     /locus_tag="WIE35_001539"
     CDS             1160..2290
                     /locus_tag="WIE35_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ4154435.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDHQDATARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2361..3212
                     /locus_tag="WIE35_001540"
     CDS             2361..3212
                     /locus_tag="WIE35_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ4154436.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVIFGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDLEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3295..3957)
                     /locus_tag="WIE35_001541"
     CDS             complement(3295..3957)
                     /locus_tag="WIE35_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ4154437.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(4052..>5516)
                     /locus_tag="WIE35_001542"
     CDS             complement(4052..>5516)
                     /locus_tag="WIE35_001542"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ4154438.1"
                     /translation="IRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCS
                     LSRAIERDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAR
                     EYTDDVEFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELI
                     AKTPNGGKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVM
                     ALKVRHDLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGV
                     LKHRETYEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFK
                     ELADKKREIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEK
                     RASATGSGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNG
                     QGADTDVLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
BASE COUNT         1168 a         1603 c         1395 g         1350 t
ORIGIN      
        1 tcaggaatga cggtttggaa attgcccgaa accccaaaaa cagaaaccgg acaaacgggt
       61 tttccgccaa agccggcatt ttccgacttt gcttcaggca tcttccccac cgcccaaaat
      121 catcgaaccg ataccggcat cggtaaagat ttccagcaaa agcgcgttgg gcaccctgcc
      181 gtcgatgatg tgcgtggctt tcacgccgtt gacggcggct tcgaccgcgg aagcgatttt
      241 cggcagcata ccgccataca gcgtgccgtc ggcaatcagt tcatcaatcc gtttcggcgt
      301 gagtttggtc agcagattgc ccgttttgtc catcacaccg gcgatattcg tcatcatcaa
      361 aagtttttcg gcgttcaatt cttccgccaa tttgcctgcc accaaatcgg cgttgatgtt
      421 gaacgcttcg cctttttcac ctacgccgac gggggcgacg acgggaatgc agccacgttc
      481 tatcagccct ttaaccaaac cggtatcgat gctttccacc gtaccgacct gtccgatgtc
      541 cacgccattc tgttcgggcg tatcaatcaa aagtttcttc gccttaatga aatggtcgtc
      601 gcgtccgctt acgccgaccg cgtgtccgcc atatgtgtta atcatcgaca cgatttcttt
      661 attgacatgc ccgcccaaca ccatttcgac aatatccatc gcctctttgt cggtaacgcg
      721 cattccttgg acaaactcac cctttttgcc gactttttca agcatcgcat tgatctgcgg
      781 cccgccgccg tgaacgatga cgggatgaat gccgaccagc ttcagcagca cgacatcgcg
      841 ggcaaaccct tctttcaagg caggttcggt catcgcgttg ccgccgtatt tgatgacggc
      901 gaccgaaccg gaaaaccggc ggatgtaagg cagcgcttcg gcaaggatac gcgccttgtc
      961 ggcggcggaa ataatgtttt cagactccat aacggctccc tattgttaac aatttcggct
     1021 tatgttaacg ccaaacgccg tctgaaacaa tcttcagacg gcatccggca gactttcggg
     1081 caggttgaag ccttgcgata cgggcggtaa aatcgcggtt tatccggttc gagaaaccgg
     1141 cgacagcaag gactttccca tgattaaaat cagcacccgc ttcgatgccg gctcggtcgt
     1201 cgtcaaagac ctgaccgatc cttccaacat ccgcctcgcc ctgcgtcccg acaacgcctc
     1261 cgattttgca caatggttct acttccggct gcaaggcgcg gcatatcaaa actgcatcat
     1321 gcattttgaa aacgcggcag aagccgccta cccgaaaggc tgggaaggct atcaggcgtg
     1381 tgcctcatac gaccgccgca actggttccg cgtaccgact tcctacgaaa acggcgtact
     1441 gaccgtcaac cataccccgc tgtccaatag cgtgtactac gcctatttcg aaccctattc
     1501 cgaagaacag cacctcaacc tcctcggcga cgcgcaaggc agcggcctgt gccgcatcga
     1561 cgacttgggc agcaccgtgc aagggcgcga catcaatctg ctgaccatag gcaatcaggt
     1621 cgaaagcgat ttgaaaatct ggattaccgc ccgccagcat ccaggcgaaa ccatggcgga
     1681 atggtttgtc gaaggactgc tcggcaggct gctcgaccat caggatgcaa ccgcgcgcac
     1741 cctgctcgat cgcgccacgt tctatatcgt ccccaacatg aaccccgacg gttcggcact
     1801 gggcaacctt cgcaccaacg ccgccggtgc gaacctcaac cgcgaatggg aaaatccgac
     1861 tttggaaaaa agccccgaag tgttcttcgt gcgcgaaaaa atgctggaaa ccggcgtgga
     1921 tttgttcttg gacatccacg gcgacgaagg gctgcccttt atctttgttg caggaactga
     1981 aggcgtgcca aactacaatc cgcgcatttc cgcgttggaa acacagttca aaaccgccct
     2041 tttgaacgcc agccccgatt tccaagacga atacggttac gaaaaagatg cgcccggtca
     2101 ggcaaacatg accttggcaa caaactgggt cggcaaccgt ttcaactgcc ttgcctatac
     2161 gttggaaatg ccctttaaag acaatgccaa cctgcccgac gacgatttcg gatggaacgg
     2221 tcaacggtcg ttgcgcttgg gcgaagccgc gttgtccgcc atcctcaaca tcatcggcga
     2281 tttgcgctga aacccttatt gcagaaatgc cgtctgaagc atcatgcctt tcagacggca
     2341 tttgaaaacg aaagcctgtt atgattatcc atccccaatt cgatcccgtc ctcatcagta
     2401 tcggcccgct tgccgtccgc tggtatgccc taagctacat cctcggattt attcttttta
     2461 cctttctcgg cagaaggcgc atcgcgcaag gcttgtccgt ttttaccaaa gaatcgcttg
     2521 acgacttcct cacttggggc attttgggcg taatttttgg tgggcgtttg ggttacgtcc
     2581 tgttttacaa gttttccgac tacctcgccc atccgcttga tattttcaag gtatgggaag
     2641 gcggaatgtc gttccacggc ggctttttgg gtgtagttat tgccatatgg ttgttcggcc
     2701 gcaaacacgg catcggcttc ctcaaactga tggacacggt cgcaccgctc gttccgctgg
     2761 gtctcgcttc gggacgtatc ggcaacttca tcaacggcga actttgggga cgcgttaccg
     2821 acatcaacgc attttgggca atgggcttcc cgcaggcgcg ttacgaagac ctcgaagccg
     2881 ccgcgcacaa tccgctttgg gcagaatggc tgcaacaata cggtatgctg ccgcgtcatc
     2941 cctcgcagct ttatcagttt gcacttgaag gcatctgcct gttcgccgtc gtttggctgt
     3001 tctctaaaaa acagcggccg accggacaag tcgcctcgct cttcctcggc ggctacggca
     3061 tattccgctt cattgccgaa tttgcacgcc aacccgacga ctatctcggg ctgctgacct
     3121 tggggctgtc gatggggcaa tggttgagcg tcccgatgat tgttttgggt atcgtcggct
     3181 ttgtccggtt cggcatgaaa aaacagcact gaccgcctgt gaaaaatacc gtctgaaaca
     3241 tggaaatccg gtttcagacg gcatttttac gtttggttca tggaaacaaa cgcttcaatt
     3301 cttaccgcaa cacgccttgt attttctgcc gctgccgcac ggacagggat cgttcctgcc
     3361 tgttttttcg ccttccctgc ggacggtttg cggcttgttg atgaccgcct gccagtagcg
     3421 gtagatatcc gccaatgcat aaggcaactc ggattccagt tccgccagct cgccttctgt
     3481 gaattgcaga cggatagtgc cgttgtcctc ttcgtcgtaa ataccgccca atgccatgat
     3541 gggataaaac aactcttcaa acgcttcatc atcgacggct tcaaaccaat cggtcggcac
     3601 aatatccaaa ccgtaaagat aagcattgca ccatgtgtaa aaatcgctgc cgccgtcttc
     3661 gttttcatac agccacaaat cgggcagttt tttatccgac atcgcggcgg ttgtttccat
     3721 cgccattgcc aaaaccagcc gttcgatttc ggaacgttcg gcggcggtaa attgcgattc
     3781 gtcgcccaac acttcgggca gccagtcgag cggtgtcaat ttgtccggcc cgctcaacag
     3841 cgccgtcata aaaccttgaa cctcgtcgca acgcatcgtg ttgccttgtt cgcttttggc
     3901 atccaacaat tcgctcaacc gccgtttgga tgcttcggta aattttcggg aatccatcat
     3961 tttccttttc aaatgggttt tgcgccctat tatcgccgca atgccgtctg aagccttttt
     4021 atgcttcaga cggcatcgtc tgttttcacg ctcaaatcgt accgctgcct tgcgctttcg
     4081 gtttggcggc actaaattcc agtttgctca aagcggaaag gtaggctttg gcggtggcga
     4141 ccaaaacgtc ggtatccgcg ccctgaccgt tgacgacgcg gttgccgcgc gccagacgga
     4201 cgctggtttc gccctggctt tccgtacctt gcgtgacggc gttgacggaa taaatctgca
     4261 aagccgcgcc gctttgcgcc acgctttcaa tcgctttgaa aatcgcatcc acggggccgg
     4321 agcctgtggc ggaagcgcgt ttttcttcgc ctttgatgct gaacacgatg tcggcgcgcg
     4381 gctcttcgcc ggtttcggtg ctgatttttt gggagatgaa tttgtagctc tcggcattca
     4441 tgctgcccat ttcgtcggat accagtgcgt gcaggtcttc atcgaagatt tcgcgttttt
     4501 tgtcggcgag ttctttgaag cgggcaaacg cggcgttcag tgcctcttcg ctttccaact
     4561 cgatgcccaa atccgccagc ttggttttga aggcgttgcg gccggacaat ttgcccaagc
     4621 tcaaacggtt tgttgcccag ccgaccgatt cggcggacat aatctcgtaa gtttcgcggt
     4681 gtttcagcac cccatcctga tggatgcccg attcatgcga aaaggcattg gcaccgacaa
     4741 tggctttgtt gggctgcacg ggatagcccg taatggtgga caccagtttg gacgaaggca
     4801 cgatttgcgt ggtatcgatg ccggtttcca agccgaacaa gtcgtggcgc actttcaacg
     4861 ccatcacgat ttcttcaacc gaagcattgc ctgcacgttc gcccaagccg ttgacagtac
     4921 attccacctg acgcgcgccg ccttttaatg cggcaagcga attggcaacc gccaagccca
     4981 aatcgttgtg gcagtgtgcc gaccaaacga ctttgccgcc gttgggcgtt ttggcaatca
     5041 gttcgcggaa aaattcttcg gttttgtacg ggattgaata gccgacggta tcgggaatat
     5101 tgatggtggt cgcgcccgct tcaatcaccg cgccgcagat ttcggcaagg aaatcgattt
     5161 ccgaacgcaa cgcgtcttcg caggaaaatt ccacatcgtc ggtgtattca cgagcgattt
     5221 tcaccgcttt gaccgccgcc tcaatcacct gcttcggctt catcttcaat ttgtactcca
     5281 tatggatggg gctggtggcg atgaaggtgt ggatgcgttt tttcggcgcg ggcgcaacgg
     5341 cctcacccgc ctgacggatg tcccgctcga tggcgcggga caatgaacag accgttgatt
     5401 tggtaatggt tttcgcaatc gcattgaccg cctcgaaatc gcccgggctg gcagcggcaa
     5461 aacccgcttc gatgatgtcc acacccaatt tttccagctg gcgggcgacg cggatt
//
LOCUS       DAWHXZ010000140         5490 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000140 DAWHXZ010000000
VERSION     DAWHXZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.140"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            70..543
                     /locus_tag="WIE35_001543"
     CDS             70..543
                     /locus_tag="WIE35_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ4154439.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            669..1772
                     /gene="mnmA"
                     /locus_tag="WIE35_001544"
     CDS             669..1772
                     /gene="mnmA"
                     /locus_tag="WIE35_001544"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ4154440.1"
                     /translation="MNTTANTTNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQSTDKPVIITR"
     gene            1843..3513
                     /locus_tag="WIE35_001545"
     CDS             1843..3513
                     /locus_tag="WIE35_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ4154441.1"
                     /translation="MEKIWLDSYEQGVNAEIDITQYGSVSDVFCQSVKKFAGQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFVIRKIKKMVPEYRIRETISFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITDTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVRGPQVMKGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLIVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVRKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            3625..5259
                     /locus_tag="WIE35_001546"
     CDS             3625..5259
                     /locus_tag="WIE35_001546"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ4154442.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGVHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     TGSLAAKIYGSGHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         1451 a         1492 c         1384 g         1163 t
ORIGIN      
        1 gtactggttt ttgttaatcc gctataagat taagcacatt ccccgtctga tattataagg
       61 agcagaaaga tgaaaaaatt attggcggcc gtgatgatgg caggtttggc aggcgcggtt
      121 tccgccgccg gagtccacgt tgaggacggc tgggcgcgca ccaccgtcga aggtatgaaa
      181 ataggcggcg cgttcatgaa aatccacaac gacgaagcca aacaagactt tttgctcggc
      241 ggaagcagcc ccgttgccga ccgcgtcgaa gtgcataccc acatcaacga caacggcgtg
      301 atgcggatgc gcgaagtcga aggcggcgtg cctttggagg cgaaatccgt taccgaactc
      361 aagcccggca gctatcacgt gatgtttatg ggtttgaaaa aacaattaaa agagggcgac
      421 aagattcccg ttaccctgaa atttaaaaac gccaaagcgc aaaccgtcca actggaagtc
      481 aaaatcgcgc cgatgccggc aatgaaccac ggtcatcacc acggcgaagc gcatcagcac
      541 taatctgctg aaaatgtttg aaatgccgtc tgaaaaagcc cgggctttca gacggcattt
      601 ttatgaccgc ctttaaaatg tgttaaaatc tgcctttaaa aaccgccgtt tccaaccata
      661 ttctacgcat gaatacgaca gcaaacacca ccaatatcat cgtcgggctc tccggcggtg
      721 tcgattcttc cgtaaccgcc gccctgctca agcagcaggg ttatcaagtg cgcggtgtgt
      781 tcatgcagaa ctgggaagac gacgacaacg acgaatattg cagcatcaaa caggattcgt
      841 tcgatgccat cgccgttgcc gatattatcg gcatcgatat cgacatcgtt aatttcgccg
      901 cgcaatataa agacaaagtc tttgcttatt ttcttcagga atacagtgcg gggcgcacgc
      961 cgaatccgga tgtgttgtgc aacgccgaaa tcaaattcaa atgctttttg gactacgccg
     1021 tagggcaggg cgcggatacc atcgccaccg gccactatgc gcgcaaagaa gttcgcaacg
     1081 gcgtgcatta cctgctcaaa ggtttggatc gaaacaaaga ccaaagctat ttcctctacc
     1141 gcctcaagcc tttccaactc gaacgcgcga tttttccgtt gggcggtttg gaaaaacccg
     1201 aagtgcgccg ccttgccgcc gagttcaaac tgccgaccgc cgccaaaaaa gacagcacgg
     1261 gcatctgctt catcggtgaa cgcccgtttc gcgagtttct gcaaaaatac ctgccgaccg
     1321 acaacggtaa aatggttacg cccgaaggga aaaccgtcgg cgaacacgtc gggctgatgt
     1381 tctacacgct cggacagcgc aaaggtttgg gcatcggcgg cgcgggcgaa ccgtggtttg
     1441 ttgcggctaa agatttgacg aaaaacgaac tgattgtcgt gcaaggacac gaccatccgc
     1501 tgctctatac ccgcagcctt gtgatgaacg atttgagttt cacgcttccc gaacgcccga
     1561 aggcaggacg ctatacctgc aaaacgcgtt accgtatggc ggacgcgcct tgtgagttgc
     1621 gctatttgga tgaagaaacc gccgagctgg tgtttgacga accgcaatgg gcggttacgc
     1681 cgggccagtc tgccgtgctg tacgacggcg acatctgttt gggcggcggc attatccaat
     1741 caaccgacaa acccgtcatc atcacacgat aaaggtaatg ccgtctgaaa cggttttcag
     1801 acggcattgt tccgctcaat tccactttaa agaccgatac ctatggaaaa aatctggtta
     1861 gacagttacg aacagggcgt gaatgccgaa atcgacatca cgcaatacgg ttccgtcagc
     1921 gatgtatttt gccaaagtgt caagaaattt gccggccaac ctgcttttca aaacatgggc
     1981 aagacgctga cttatgccga aaccggcaaa ctggcaacag attttgcttc ttatctgcaa
     2041 aacgtcctga agctgccgcg cggcgaacgt gttgccatta tgctgcccaa cgtattgcaa
     2101 tacccgattg cccttttcgg cattttgcag gcaggtttgg tagcggtgaa caccaatccg
     2161 ctctataccc cgcgcgagct ggagcatcag ttgaaagaca gcggcgcgac cgccatcatc
     2221 gttttggaaa atttcgccaa cacgctggag ctggtgctgc cgcgcacgca gatcaaacac
     2281 gtcatcgtag cctccgtcgg cgaaatgttc gggctgctta aaggttcgct gatcaatttc
     2341 gtcatccgga aaatcaagaa aatggtgccc gaataccgta ttcgggaaac catttccttt
     2401 cagacggcat tgaaagaggg ggcgaagcac gttttccaac ctgtcgcatt aaaccgcgaa
     2461 gataccgcgc tgttgcaata cacgggcggc acgacaggtg ttgccaaagg ggcggtgctg
     2521 agccacggca acatctgcgc caatatgctt caggcaaaag aatggattaa aaaccagttg
     2581 cgtgagggaa aagaaaccgt tatcgccgcc ttgccgctgt accatatttt tgccttaacc
     2641 gtgaatctga tgatttttgc caatgccggc tcgaaaattg tcctgattac caacccgcgc
     2701 gatatgaaag gctttatcgg agaactgaaa aaacagcggg ttaatgtatt tatcggcgtg
     2761 aacacgctgt ttaacgcgat ggttaaccgt cctgattttg ccgaagtcga tttttcagga
     2821 ttgcggctga ctttgggcgg cggtatggcg acccaaaaag ccgttgccga aaaatggaaa
     2881 aaaatcaccg acacgcccat cgtcgaagcc tacggtctga ccgaagccag ccccggcgtg
     2941 tgctgcaacc ccttaaacat cgaatcatac agcggcagca tcggtttgcc cgtctcgtcc
     3001 accgaagtcg aactgcgcga cgcaaacggc aaagaagtcc ccgtcgggca gccgggcgaa
     3061 ttgtgggtgc gcggcccgca agtcatgaaa ggctattgga accgtcccga agaaaccgct
     3121 aaagccatag acgcgcgcgg tttcttggaa accggcgata ttgccgtcat ggacgaaaaa
     3181 ggctggttga agctggttga ccgtaagaaa gacctcattg ttgtttctgg ttttaatgtt
     3241 tatccgaacg aaatcgagga agtcatcgcg catcacggca aagtgatgga agtcgcctgt
     3301 atcggcgttc ccgacgaaaa aaccggagaa gctctcaaag tgtttgtcgt cagaaaagat
     3361 ccgtctttga cgaaagaaga acttatcgcc ttttgccgca ccgaattaac cgcatataaa
     3421 gtaccgaaaa atatcgaatt ccgcgacgag ttgcccaagt ccaacgtcgg caaaatcctg
     3481 cgccgcgagt tgcggcaaag tactgggaaa taaagaaaag atgccgtctg aaaacagccg
     3541 tccaccgttc agacggcatc cgtccgtttg ccagaaccgc gcgcttcaag ttaaaatcgc
     3601 acattccaac atgggtattc catcatgacc aaattcattt tcgtaaccgg cggcgttgtc
     3661 tcctcactgg gtaaaggtat cgccgccgct tctattgccg ctatcctcga atcgcgcggc
     3721 ttgaacgtta ccatgctcaa gcttgatcct tatatcaacg tcgatcccgg cacgatgagc
     3781 ccgtttcaac acggcgaagt gttcgtaacc gacgacggcg cggaaaccga cctcgactta
     3841 ggacactacg aacggtttat cgattccacg atgacccgcc gcaacagctt cagcacgggg
     3901 caggtgtacg aaaacgtcat cgccaaagaa cgacggggcg actacctcgg cggcacggtt
     3961 caagtcatcc cgcacattac cgacgaaatc aaacgccgca tccacgaagg cgcggcaggt
     4021 tacgatgtgg cgattgtcga aatcggcggc acggtcggcg acatcgaatc gctgccgttt
     4081 ttggaagcca tccgccagat gcgaagccag ttgggacgca acaacaccct gttcgcccac
     4141 ttgagctacg tcccctacat cgccgctgca ggcgaaatca aaaccaagcc gacccagcac
     4201 accgttaaag aaatgttgag catcggtttg caacccgaca tcctgatttg ccgtatggac
     4261 aggacaatgc ctgcggacga acgccgcaaa atcgccttgt tctgcaacgt ggaagaacgc
     4321 gcgattgtcg gcagctacga tgtggacagc atctacgaat gccccgaaat gctgcacgac
     4381 caaggcatcg acaacatcat taccgagcag ttgcagctta atgtgcagca ggcggatttg
     4441 accgcgtgga aaaaaatcgt ccacgccatc caaaacccga aacacaccgt caaaatcgcc
     4501 atggtcggca aatacgtcga tttgaccgaa tcctacaaat cactgattga agccttgaaa
     4561 cacgcaggtg ttcacaccga aaccgacgta caaattacct ttgttgacag cgaaagcatc
     4621 gagaaaaaca acggcgacgt ttctatgctc aaagatatgg acgccatcct cgttcccggc
     4681 ggtttcggtt cgcgcggcgt ggaaggcaaa atcgccgctg tgcgctatgc ccgcgaaaac
     4741 aacgtgccat acttgggcat ctgcctcggt atgcagattg cgctgatcga atacgcccgc
     4801 gacgtggcag gtttgaaagg tgcgaattcc accgagttcg acctcaaatg cgccgccccc
     4861 gtcgttgccc tgattgacga atggcaaact gccgacggca gcgtcgaaac ccgcgacgaa
     4921 tccgccgatt tgggcggcac catgcgtttg ggcgcgcaag aagtcgaatt gaaaacaggc
     4981 agcctcgccg ccaaaatcta cggcagcgga cacatccgcg aacgccaccg ccaccgctac
     5041 gaagtcaaca acaactatgt tcctacgctg gaacaggcag gtttggtcat cggcggcgta
     5101 tccgccggac gcgaacgctt ggtcgaaacc atcgaactgc cgaaccatcc ttggttcttc
     5161 gcctgccagt tccaccccga gtttacctcc aatccgcgca aagggcatcc tttgttcacc
     5221 gcgtttgtca aagctgcgtt gaacaataaa aaagcctgat aaagcgttac ttgatgatca
     5281 aaatgccgtc tgaaagcctg aatgaagact ttcagacggc attttcgcaa atcggggatt
     5341 ataaaagcca tcaatttaat ttttatggtt ttatctaata tttaccaact gaatattgtt
     5401 aagcggggcg tgaaactcaa gttgagacct ttgcaaaatt ccccaaaatc ccctaaattc
     5461 ccaccaagac atttaggagc accttcttcc
//
LOCUS       DAWHXZ010000141         5489 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000141 DAWHXZ010000000
VERSION     DAWHXZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.141"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..744
                     /locus_tag="WIE35_001547"
     CDS             <1..744
                     /locus_tag="WIE35_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4154443.1"
                     /translation="KLGVKTGLSIDKNRLPYLDEYYKTTKPAGTLFEPDYEALNAYKP
                     QLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDALAQIFGKQAEADKLKA
                     EIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWLHKDIGVPAVDEAIKEG
                     SHGQPVSFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNPLVAETTAWKKGQVVYL
                     VPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            908..1877
                     /locus_tag="WIE35_001548"
                     /pseudo
     CDS             908..1877
                     /locus_tag="WIE35_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            1867..2841
                     /locus_tag="WIE35_001549"
     CDS             1867..2841
                     /locus_tag="WIE35_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ4154444.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELAVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            2886..3512
                     /locus_tag="WIE35_001550"
     CDS             2886..3512
                     /locus_tag="WIE35_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154445.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAFMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGVGPAGRFARRNLLSFGAGEINGTLALRRRDTGKTVAVSLNAALQPFAPE
                     MRELMPKTVGGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            3596..4354
                     /locus_tag="WIE35_001551"
     CDS             3596..4354
                     /locus_tag="WIE35_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ4154446.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLACTPTAELAKTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDFADRYLTELSGGQRQRAMIAMVFCQRTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQVAMQG
                     KPDDIFTAANIKTLFDMDVSVLDHQSKKLIVHHI"
     gene            complement(4413..4751)
                     /locus_tag="WIE35_001552"
     CDS             complement(4413..4751)
                     /locus_tag="WIE35_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ4154447.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            4846..5058
                     /locus_tag="WIE35_001553"
     CDS             4846..5058
                     /locus_tag="WIE35_001553"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154448.1"
                     /translation="MPETVIPAQAGIPISAFQQYLKVSVNPNFHIPNRTETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            5215..>5489
                     /locus_tag="WIE35_001554"
     CDS             5215..>5489
                     /locus_tag="WIE35_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154449.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWA"
BASE COUNT         1159 a         1636 c         1440 g         1254 t
ORIGIN      
        1 aaattgggcg tgaaaaccgg tttatccatc gataaaaacc ggctgcctta tttggacgaa
       61 tattacaaaa ccacgaaacc tgccggaact ttgttcgagc cggattacga agccttaaac
      121 gcctacaagc cgcagctcat catcatcggc agccgcgccg ccaaggcgtt tgacaaattg
      181 aacgaaatcg cgccgaccat cgaaatgacc gccgataccg ccaacctcaa agaaagtgcc
      241 aaagagcgta tcgacgcgct ggcgcaaatc ttcggcaaac aggcggaagc cgacaagctg
      301 aaggcggaaa tcgacgcgtc ttttgaagcc gcgaaaactg ccgcgcaagg caaaggcaaa
      361 ggtttggtga ttttggtcaa cggcggcaag atgtcggctt tcggcccgtc ttcacgactg
      421 ggcggctggc tgcacaaaga catcggcgtt cccgctgttg acgaagccat caaagaaggc
      481 agccacggcc agcccgtcag ctttgaatac ctgaaagaga aaaatcccga ctggctgttc
      541 gtcctcgacc gaagcgcggc catcggtgaa gagggtcagg cggcgaaaga cgtgttggat
      601 aatccgctgg ttgccgaaac aaccgcttgg aaaaaaggac aagtcgttta ccttgttcct
      661 gaaacttatt tggcggccgg tggcgcgcaa gagctgctga atgcaagcaa acaggttgcc
      721 gacgctttta acgccgcgaa ataatgaaac ggcggcattc gatgccgtct gaaacacgga
      781 tgcaaaccgc ttcctgtgtt tcagacggca ttgcccgata cggaggcttc aaacaaggct
      841 ttccgctccg acggttcgga ctgccttgtt tgaatcttct acgccgtaac gcttttccct
      901 tctgtttatg actgccaaac ctttttccct caacctgact aacctcctgc tgctggcggt
      961 gttgtttgcc gtcagcctgt cggtcggcgt tgccgatttc cgctggtcgg atgtgttttc
     1021 gctgtccgac agccagcagg ttatgttcat cagccgcctg ccgcgcacgt ttgcgattgt
     1081 gctgacgggc gcgtcgatgg cggtggcggg gatgattatg cagattctga tgcgcaaccg
     1141 ttttgtcgaa ccttctatgg cgggcgcgag ccaaagtgcg gctttgggtt tgcttctgat
     1201 gtccctgctg ctgcctgccg cgccgctgcc ggtcaaaatg tcggttgccg ccgttgccgc
     1261 gctaatcgga atgctggtgt ttatgatgct tatccgccgc ctgccgccga cggcgcaact
     1321 gatggtgccc gctggtgggg attattttcg gcggcgtggt tgaggcggtg gcgaccttta
     1381 tcgcgtatga aaacgaaatg ctgcaaatgc tcggcgtgtg gcagcagggc gatttttcga
     1441 gcgtgctgct ggggcggtac gagctgcttt ggattacggg cggtttggcg gtgtttgcct
     1501 atctgattgc cgaccggctg acgattttgg ggctgggcga gacggtcagc gtgaatttgg
     1561 gtttgaaccg gacggcggtg ttgtggtcgg ggctgattat tgtggctttg attacgtcgc
     1621 tggttatcgt tacggtcggc aatattccgt ttatcgggct ggtcgtgccg aacatcatca
     1681 gccgcctgat aggcgacagg ctgcgccaaa gcctgcctgc ggtggctttg ttgggcgcgt
     1741 ctttggtttt attgtgcgat attttgggac gcgtgattgt gtttccgttt gaaattccgg
     1801 tctcgaccgt cttcggggta ttgggtacgg cgttgttttt gtggctttta ttaaggaaac
     1861 ctgcccatgc cgtctgaaaa aaatatcggt tttatggcag gaagcagccg tccgttgtgg
     1921 gtagcttttg cattgttgct ggtttcctgc gtcctgttta tgacgctcaa cgtcaaaggc
     1981 gattgggatt ttgtactgca cctgcgtctg accaaacttg ccgcgctgct gatggtcgcc
     2041 tatgcggtcg gcgtgtccac gcaactcttc caaacgctga ccaacaatcc gattctgacc
     2101 ccttcaattt tgggtttcga ttcgctgtat gtgtttttgc agaccttgct ggtgtttacg
     2161 ttcggcggcg tgggctatgc ttccctgccg ttgacgggca aattcggctt tgaacttgcc
     2221 gttatgatgg gcggctcgct gctgctgttt tacacgctca tccgtcaggg cggacgcgat
     2281 ttgccgcgta tgattttaat cggcgtgatt ttcgggattt tgttccgcag cctgtcgtcg
     2341 ctgctttcgc gcatgatcga ccccgaagaa tttacggcgg cgcaggcgaa tatgtttgcc
     2401 ggattcaata ccgtccacag cgagcttttg ggcataggcg cgctgattct gctcgtcagc
     2461 gcggcggtcg tttggcgcga acgctaccgc ttggacgtac accttttggg gcgcgaccaa
     2521 gccgtcaatt tgggcatcag ctacacgcgc aacaccttgt ggatactgct ttggattgcc
     2581 gcattggtgg cgacggcgac cgccgtggtc ggcccggtaa gctttttcgg gcttctcgcc
     2641 gcctcgcttg ccaaccactt ttccccgtcc gtgcgccatt ccgtccgcct gccgatgacg
     2701 gtttgtgtcg gcggcatcct cctggtcggc ggacagaccg tattcgaaca cttcttgggc
     2761 atgaaggcgg tattaagcgt ggtggtcgaa tttgcgggcg gactcgtttt cctctatctc
     2821 gttttaaaac acaaaaaatg acggatgcca tctgaacggc agcccgcccc gaaaggacaa
     2881 accatatgac gcaagaacgt ttacccgaat ttttcgaccg cgccccgacg ctgaccgtac
     2941 aagacccgct tgccgcattc ctcggcgcgg ccgaaaacgg catcctcact taccgctacg
     3001 ccgatgccgt gcgcctgtgc ggacattcct gcccgaccgt cgcgggcgcg tacctgatgg
     3061 ttatcaaagg tctgaaagca ctttacggcg aagagctgcc cgaacgcggc ggcatcgaag
     3121 cctttatgca gggcgcgcgc gacgaaggca cggtcggcgt aaccgcgtcc gtcgtccaac
     3181 tccttaccgg cgccgcaccc gaaaccggct ttggcggcgt aggccctgcc ggacgctttg
     3241 cccgccgcaa cctcttgtcc tttggtgcag gcgaaatcaa cggcacactc gcgctccgcc
     3301 gccgcgatac cggcaaaacc gtcgccgtca gcctcaacgc cgccctgcaa cccttcgcac
     3361 ccgaaatgcg cgaacttatg cccaaaaccg tcggcggcag cgcaagcgca gaagaactcg
     3421 aacgcttcgg acaactctgg caggcacgcg ttaaagcatt tttgaccgaa tcggcggacg
     3481 acccgcagtt cgtcatcgtc cgcgaagtgt gagcgttcag acggcattcc gaatttcaaa
     3541 tgccgtccga acccccgcca aacaacaaca aacctacgcc cgacaagcat ccgccatgat
     3601 taccatccgc aacgtcagct accgcatcgg cacacgcccc atcctcgaca acgtcagcct
     3661 cgacatcccc gaaggcggca ttaccgccct cgtcggcccc aacggtgcgg gcaaatccac
     3721 cctgttttcc tttatggcgc ggctgcgccc gcttgaaagc ggcagcatcg cctaccgcgg
     3781 caaaaacctt gcctgcaccc ccaccgccga actcgccaaa accctgtcca tcctcaccca
     3841 agaaaacagc atcatgagcc gcatcaccgt gcgcgacctg ctcatgttcg gccgctatcc
     3901 ctaccatcaa ggcagaccga ctgccgaatg ccgccgtatc gttaacggtg caatcgaaga
     3961 attccacctg caagacttcg ccgaccgcta cctgaccgag ctttccggcg gacaacgcca
     4021 acgcgccatg attgccatgg tgttctgcca gcgcaccgac tacgttttgc tggacgaacc
     4081 gctgaacaac ctcgacatgt atcatgcccg ctccctcatg caaatcctgc gccggctgac
     4141 cgacgaacac aagcgcacca ccgtcgtcgt attacacgac atcaaccagg ccgccgccta
     4201 cgccgaccac gtcgtcgcca tgaaaaacgg ccaagtcgcc atgcagggca agcccgacga
     4261 cattttcacc gccgcaaaca tcaaaaccct gttcgatatg gatgtgtccg tcctcgacca
     4321 ccaaagcaaa aaactcatcg tccaccatat ctgaagctca aaaagaaaat gccgtctgaa
     4381 aacctttcag acggcatttc cattccaaac gctcaaaccg ccgcgtccgc acgttcgccc
     4441 gtgcggatac ggattgcctc ctcgaccggc agcacaaaaa tcttgccgtc gccgattttt
     4501 cccgaacgcg ccacctcgac aatcacgtca atcgcgcgtt ccacagcgtc atccgccaac
     4561 accagctcga ttttgacctt gggcaggaaa tcgacagcgt attccgcgcc ccggtaaatc
     4621 tccgtatgcc ccttctgcct gccgaaccct ttgacctcgc tgacggtcat acccgtaatg
     4681 ccgatttccg tcaacgcctc gcgcacgtcg tcgagtttga acggtttgac aatcgcctcg
     4741 atttttttca tcacattctc cattaattaa cgtaaacaaa cgacaaatat agcagaaacc
     4801 gccggcaaac gataaacggt actgttcgcc cggcgatgcg gcgatttgcc ggaaaccgtc
     4861 attcccgcgc aggcgggaat cccgatctct gcatttcagc aatatttaaa ggtttctgta
     4921 aatccaaact tccatattcc taaccgtacg gaaacgacgg catttgatct aaaaacatac
     4981 ggaagcagtt gcccgcccct ctacttcctg caattatccg catacccggc tttgggtaaa
     5041 aatcagaaaa acagataaaa cccttattcc ttaatatctt cagacggcac ctgtattttc
     5101 cccaactgta tgccgtctga agattgtcaa cattttaagc caaaataccc aagcaataca
     5161 gccccgttgc gcgtataatg acagattttc caaccgcatt tgagagccga atccatgtct
     5221 gtcgttttgc ccttgcgcgg cgttaccgcc ctttccgatt tccgtgtcga aaaactcttg
     5281 caaaaagccg ccgcactcgg actgcccgaa gtcaaattaa gcagcgaatt ttggtatttc
     5341 gccggcagcg agaaagcatt ggatgccgcg actgtcgaaa aactgcaagc cttgttggcg
     5401 gcgcaaagcg ttgaacaaac gccaaaagcg cgcgagggct tgcatttgtt tttggtaacg
     5461 ccccgtttgg gtacgatttc gccgtgggc
//
LOCUS       DAWHXZ010000142         5479 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000142 DAWHXZ010000000
VERSION     DAWHXZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.142"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(79..315)
                     /locus_tag="WIE35_001555"
                     /pseudo
     CDS             complement(79..315)
                     /locus_tag="WIE35_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
     gene            511..969
                     /locus_tag="WIE35_001556"
                     /pseudo
     CDS             511..969
                     /locus_tag="WIE35_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            869..1237
                     /locus_tag="WIE35_001557"
     CDS             869..1237
                     /locus_tag="WIE35_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ4154450.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDNLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLVGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            1306..2844
                     /gene="lnt"
                     /locus_tag="WIE35_001558"
     CDS             1306..2844
                     /gene="lnt"
                     /locus_tag="WIE35_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ4154451.1"
                     /translation="MFSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFSLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3108..3971
                     /gene="rpoH"
                     /locus_tag="WIE35_001559"
     CDS             3108..3971
                     /gene="rpoH"
                     /locus_tag="WIE35_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ4154452.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(4030..4176)
                     /locus_tag="WIE35_001560"
     CDS             complement(4030..4176)
                     /locus_tag="WIE35_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154453.1"
                     /translation="MLIHYINFANLSEKQLMRQCLVHVCFLISAIAMAWTDKIVYGTT
                     HKPH"
     gene            4455..5339
                     /locus_tag="WIE35_001561"
     CDS             4455..5339
                     /locus_tag="WIE35_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ4154454.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDTENSRQSLELNSDLVNQLAEINKNLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLVEAVGDINNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIDEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
BASE COUNT         1411 a         1636 c         1253 g         1179 t
ORIGIN      
        1 cgaccttgcc gtcgggcagg atgtaccacg ccaaatcccc gcctgaagca cggtgtcgga
       61 ctgcatttcg attcggctat acgccttttc acgcaggctg taattgaatt tccacaaacc
      121 gccgaacact gccggcggac gcgcgaccag catcaggaac gccgcaatcg ccaaggcttc
      181 cgccgccccg tccaacacgc cggcgggaga aaccagcagc aaaggcattc ccaaacgtgc
      241 gggtaaagtg gtcgaaacca cgctcagaaa caacagtgcg ccacccggca ggaacagact
      301 gcccatccaa tccacgctaa cgattctccc ttaaaattca tgaaattaaa atcgaaagac
      361 tgtaacacgg cattttcttt tcacgaaact ttacttcacg tccgccacag cgttcagacg
      421 gcattttcct gtttcatgcc gcctgaaacc gcccaaatcc gcgttctcct atataatgtc
      481 ggactttgcg ccgcatttca ggattcttta atggcatttg cctcactatt taccctgccg
      541 gacgacatca ccgccgtttt ggacgatgtt gccctgatga ccaaagccgc cgcaaagaaa
      601 accgccggcg tggtcggcga cgatttggca cccaatgcca accgggttac gggcgtatcg
      661 gcagaacgcg agttgccgat tgtttggtcg atggcgaaag gttctttttc caacaagccg
      721 gttttggtgc ctgcggccct gctgctttcc gccttcctgc cgcaactcat tacgccgctt
      781 ttgacggcag gcggcattta tctgtgcttt gacggcgtag aaaagctgct gcacaagttt
      841 ctgcaccgac atgacgcgca tgctcgtaat ggcggcaatc ggcatcggca tgactgtttt
      901 ggtgtacgga atcgttgccg tcatcgtcaa actcgacaac ttaggcatgc tgctgatgcg
      961 ccgcccacaa accttttcgc gcagtttggg gcaaatgctg actgccttta tgccttgctt
     1021 tatgcgcggt ttgagcgtgg tcggcacgct tgccatgttt cttgtcggcg gcgtactggt
     1081 cgcccacaac ctcggccttc tgcacgattt cctgcacgcg caacactggg acgctggctg
     1141 ggcggagtac ttcgccaacc tcgttgtcgg gctgctttcc ggttcgattg cctgcgcccc
     1201 cgccttgccg ctgatgaatc gtttcggcag gcattgattt ctttttcaaa taccgatgcc
     1261 gtttgaaaga tgttcagacg gtatcttccg aacagacaga tgaatatgtt ttccaaactg
     1321 gacaaatact ggcagcaccc cgccctctac tggcctttgc tcatcctttt tgccgccgcc
     1381 acccccttta ccttcgcacc ctactaccac ttttggctga tgcccttgat tttcggtgcc
     1441 ttcgtccgcc tcatcgaact gcgtccgcgt tttgctgtct cttccgccta cctgttcagc
     1501 ctgaccgcat acacgacaca gttctactgg atacacaccg ccctgcacga cgtttccggc
     1561 ctgcccgacc tctatgccgt accgctgact ttcctactcc ccgcctacct tgccctttat
     1621 ccggcactgt gtttctggct gtggaaaaaa tttaccctgc ctcggggcat aaaaatcggt
     1681 ttggtactgc ccatcctgtg gacactgacc gagtttgccc gcgaacgttt cctgaccgga
     1741 ttcggctggg gcgcaatcgg ctactcccaa atcaccccgg acagcccgct cgccggcttt
     1801 gccccattgg gcggcatcca catggttaca ctggcaaccg cctttctcgg tgtctggctg
     1861 gttttggcga gtgataacac cgcacgttcg ggcaaacgcc tgcttccaat tatcctgatt
     1921 gccgccctgc ttgccgcagg ctacaccgcc cgacaaaccg acttcacccg ccccgacggc
     1981 agccgcagca ccgtcgccct gcttcaaggc aacatcgacc aaaccctcaa atggcgtgaa
     2041 gaccaagtca tcccgaccat acagaaatat tacgaacagg tcggcaaaac cactgccgac
     2101 atcgtcatcc tgcccgaaac cgccatcccc gttatgcgcc aaaacctgcc ggaaaacata
     2161 ctggcaaaat ttgccgaaca ggcgcaaaac aacggcagcg cgctcgccgt cggcatcagc
     2221 caatacactt cggacggcaa cggttacgaa aacgccgtca tcaacctgac cggttatcag
     2281 gaaaacaatc aggacggcat cccctactac gccaaaaacc acctcgtccc cttcggcgaa
     2341 tacaaaccgc tgcccttcct gaccacgccg ctttacaaaa tgatgaatat gcccctttcc
     2401 gacttccgca aaggcggcgg taagcaatcc gccctgctga tgaaaaacca aaaaatcgcc
     2461 ttcaacatct gttacgaaga cggattcggc gacgaactga ttgccgccgc caaagatgcc
     2521 accctgcttg ccaatgccag caatatggcg tggtacggaa aatccaacgc catgtaccag
     2581 cacctccaac aatcgcaggc gcgggctatg gaactcggac gctatatggt ccgtgccacc
     2641 aacaccggcg caaccgccat catctccccc aaaggcaaca tcatcgccca agcccaaccc
     2701 gatacggaaa ccgtattgga aggacacatc aaaggctatg tcggcgaaac gccctatatg
     2761 aaaaccggca gttcatggtg gttgatgggc atattgaccc tagccgcact gattcttttc
     2821 atcttccgaa acaaagaaca ctgatacctg cacagcccgt ccggacgggc tgtctttttt
     2881 caaaacggct atttacatac aattacgtat gtaatataat tctgaaatcg aattgagcca
     2941 tcaggtttac aaaatcaatc aaacctgcct tacaaaattt tcctgtcttt caaaccttag
     3001 cgcatatctc ctgccaaaca tacaaaatac aagccaagca gtgatatact gcatcccaac
     3061 acagcaaacc gccgatacgc agtttcagcc gaaaggaaat gccccaaatg aataacgctt
     3121 tcgcattacc cgccatccaa agcggcaacg gcagcctcga acaatacatc cacaccgtca
     3181 acagcatccc gatgctgtcc caagaagaag aaacccgcct cgccgaacgc cggataaagg
     3241 gcgacctcaa cgccgccaaa caactcatcc tgtcccacct gcgcgtcgtc gtttccatcg
     3301 cgcgcggcta tgacggctac gggctgaatc aggcagacct gattcaggaa ggcaatatcg
     3361 gactgatgaa ggcggtcaaa cgctacgagc caggtcgagg cgcgcgtctg ttttcatttg
     3421 ccgtacactg gattaaagcc gaaatccacg agtttatttt gagaaactgg cgtttggttc
     3481 gcgttgccac caccaaaccg caacgcaagc tgtttttcaa tttacgcagt atgcgtaaaa
     3541 acctcaacgc cctgtctccc aaagaggcac aagacatcgc cgacgatttg ggtgtcaaac
     3601 tttccgaagt tctggaaatg gaacagcgca tgacgggaca cgacatcgcc atcatggcag
     3661 acaacagcga cgacgaggac agtttcgccc ccatcgactg gcttgccgac cacgattccg
     3721 aaccgagccg acaactatcg aaacaggcgc attacgccct gcaaacagaa ggtttacaaa
     3781 acgctttggc gcaactggat gacaggagcc gccgtattgt ggaaagccgc tggcttcaag
     3841 atgacggcgg tctgacgctg caccagcttg ctgcagaata cggcgtctct gccgagcgca
     3901 tccgccagat tgaagcgaaa gccatgcaga aactgcgtgg tttcctgacc gaagaagccg
     3961 aagcggtttg aaatatacgc aataaaatac ccgacaaatt gttttgtcgg gtattttatt
     4021 tggtaaacat caatgcggtt tgtgcgtcgt gccgtacacg attttatccg tccaagccat
     4081 cgcaatcgcc gaaatcaaaa agcagacatg caccagacac tgccgcatca gctgcttttc
     4141 cgacagattg gcgaaattga tatagtggat taacaaaaat caggacaagg cggcgaagcc
     4201 gcagacagta caaatcgtac ggaaccgatt cgcttggtgc ttcagcacct tagagaatcg
     4261 ttctctttga gctaaggcga ggcaacgccg tactggtttt ggttaatcca ctatacattt
     4321 cccttccttg cagcaacaaa agcgcgtgat tccaatctgc cgctgcaaaa tcttcaattt
     4381 ttcacacccg aaaacgcttg ccgccataca gatgtgatat attgattcca ttttaacggg
     4441 cttcggagaa cgacatgagc agcggaaata tccacatcca cagcatgacc ggatttgcca
     4501 acgcggcggc agagtgcggc agcaaacgca tcaatctcga catccgcgcc gtcaaccacc
     4561 gctttttgga cattcaaatc aggatgccgg atgatttgcg ctatttggaa agcggtatcc
     4621 gcgagaaaat ttcatcccat atcgcgcgcg gcaaagtcga atgcaaaatc caaattcaag
     4681 atacggaaaa cagcagacag tccttggaac tcaactcgga tttggtgaac cagcttgccg
     4741 aaatcaacaa aaacctccgc aagcatcacg atttggcaaa actgggcgtt gccgacatcc
     4801 tgcgtttccc cggcgtattg gcaagccaaa gggaaaatac ggaggaattg gcaaaaagca
     4861 ttaccgaact gaccgaaaaa gcgttaaaag actttactgc cgcacgccgg cgcgaaggga
     4921 aaaaattggg cgagcacctg ttacaacgcc tcgaaagcat ggaagaaatc atagatgcat
     4981 tgagcgaact ctttcctacc ctgctggaaa cacataaaga aaaaatccgc gcccgcctcg
     5041 tcgaagcagt cggcgacatc aataatgaca ggctgcaaca ggaatttgcc ctctttattc
     5101 aaaaatccga catcgacgaa gaattcagcc gcctgcgcac gcatatcgac gaagtacgcc
     5161 gcatcgttac cgaacacaaa ggcagcagcg gaaaacgctt ggatttcctg atgcaggaat
     5221 taaaccgcga agccaacact ttgggcagca aatccattgc cgccgaatgc acccaagcct
     5281 cggtcgaact gaaagtctta atcgagcaga tgcgggaaca agtgcagaat atcgaataat
     5341 cggtttcccc gataaccaaa tgccgtctga aagccctttc agacggcatt ttttcaggtt
     5401 gaaacctttg cggtataaag gcttaaggct ttcctttgac cgccaccaat cttgttcctg
     5461 caagaaaatc atacagaaa
//
LOCUS       DAWHXZ010000143         5427 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000143 DAWHXZ010000000
VERSION     DAWHXZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.143"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..954)
                     /locus_tag="WIE35_001562"
     CDS             complement(<1..954)
                     /locus_tag="WIE35_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ4154455.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAI"
     gene            complement(989..1453)
                     /locus_tag="WIE35_001563"
     CDS             complement(989..1453)
                     /locus_tag="WIE35_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ4154456.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPSRNIKLLVFGLLIAFLGYLLGKIF"
     gene            complement(1613..1951)
                     /gene="erpA"
                     /locus_tag="WIE35_001564"
     CDS             complement(1613..1951)
                     /gene="erpA"
                     /locus_tag="WIE35_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ4154457.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(2083..2769)
                     /locus_tag="WIE35_001565"
     CDS             complement(2083..2769)
                     /locus_tag="WIE35_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4154458.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            2957..3229
                     /locus_tag="WIE35_001566"
     CDS             2957..3229
                     /locus_tag="WIE35_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154459.1"
                     /translation="MKKTGKYLIYTAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            3437..5365
                     /gene="dnaK"
                     /locus_tag="WIE35_001567"
     CDS             3437..5365
                     /gene="dnaK"
                     /locus_tag="WIE35_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ4154460.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
BASE COUNT         1584 a         1458 c         1163 g         1222 t
ORIGIN      
        1 gatggcgaga taacgtttgt cgtaatcggt cagcgtgccg acgatgccga aatcgagggc
       61 gatgtagcgg ttgtcggcgg caaccaaaat attgccgggg tgcatatccg cgtggaaaaa
      121 gccgtcgcgg aagacctgcg tgaaaaagat ttccacgccg taatcggcga gtttgtgcaa
      181 atcgatgccg tctgctttga gtttggcgat gtcggcaacc ggcgtgccgt ccatccattc
      241 gattgtcagc acgtcgctgg tgcagtagtc gtaaaacacc ttgggcacaa tcagcatatt
      301 gctgttttgg aaattgcgcc cgagctgtcc ggcattggcg gcttcgcgca tcaaatccaa
      361 ctcgtcgtgc aggtatttgt cgaactccgc caccacttcg agcggcttca gacgcctgcc
      421 gtctgaaaac aagcgttcga cccaggccgc gccgaaacgc atcagcgaca aatcctgttc
      481 gataaggggc aaaaggttgg ggcgcaaaac tttaaccgcc actcgttcgc ccgaatgcag
      541 gcgggctttg tgtacttggg cgatggacgc gctggcgatg ggctcggttt caaattccgc
      601 atacagcttt tcgatggact gacccaacga tttttcgatt tgttcacgcg aaagccgcgc
      661 gtcaaaaggc ggcactttgt cttgcagctt tgccagttcg acggcgtaat cgtgcggaat
      721 caaatcgggg cgtgtggaca aaacctgccc gaacttgatg aaaatcggcc ccaggctttc
      781 caaggcaagg cgcagacgga cagcaggcgg ttcgtttttc aatttggacg actgcggcat
      841 cattttcagc agagcgcata tccaaccgct acccatcagc gaagcacaca gacctgccag
      901 ccgatagcgg taaaaagtcc cgacaataac cgtcaggcgt ttcaaccatt tcatatataa
      961 aatcccgaat acagcattta tcggacaatc aaaaaatctt accgagcaga tagcccaaaa
     1021 aggcaatcag cagcccgaaa accagcagtt tgatattgcg ggacggcggt tcgggttgcg
     1081 aaggtgcgga aatatcatct tcccgcccgc cgtgaagttc aagatattcg ggcatttccc
     1141 catccgcgcc ttctccttcg ccctcccctt cctccggcgg cacgaccgtt tccaactcca
     1201 tatatctccg ggtaataata tccaaaccca aatagctgaa ataatcatgc aactcttccc
     1261 gtttcgccca agtgcatttt tcctcaacca gcacttgagg tttggaatcg agcgattcga
     1321 tcaatccttc cgccttttcc tcgccgagat tctccttaag gcactcccgg ataagcccgc
     1381 gatccacttg gggcgggtaa gaaacatata catcgtacaa atcgtttcgg taatctttac
     1441 tcatctcaat cataagggaa caaatacgcc tgataccgtt ccgcccgttc ctttcgggaa
     1501 acccgattca tcttcaaaca atcggattat atgtgaattc atcccgataa tatattttct
     1561 aaaagtaaaa atgccgtctg aacgttcaga cggcatcaca caaaccaagc ggtcatacgg
     1621 aaaacgacga tccgcaaccg caggttgttt ccgcattcgg attgcggatg acgaattgcg
     1681 aaccctgcaa actttccgta tagtcgattt ccgcaccgac cagatattga tagctcatcg
     1741 gatcgaccaa aaagaccaaa ccgtttttct caatttcaaa atcgtcgtcg tttttgattt
     1801 cgtcaaaagt aaatccgtac tggaaacccg aacagccgcc gccattgaca aaaacccgca
     1861 atttcaaatc gggattgttt tcttcggcaa tcaaatcggc aactttggca cagcagctgt
     1921 cagtaaaaat aatagggctt tcatccgaca tggtgttatt ccttaatata atattttctg
     1981 cattctctct caaaccccct tcaaaggcaa gcaggcttcg cctttgaaaa aggaattgtt
     2041 ccaatagata agtttgaccg cctgcaattt caagccccaa actcaaatcg tcctgccgaa
     2101 ccactcgaca cgcccgataa tctccacatc atcggtcaaa tcgttcaaat tgatttcaaa
     2161 agcaggataa gcctcgtttg cagaaatcac attgataatc ccgcccggta caatctgtaa
     2221 acgtttaacc agcagatttt cattaatccg caacacatac agaccgtccc tcggcgtatt
     2281 ttcaccatga ttgaccaaaa tcgaatcgcc gtcattcaaa accccctcca tcgaatcccc
     2341 cttgacggaa attacagaca ggtttttcgt atcgcgggta acataattct caatccaatg
     2401 ccgtctgaac gccattgtaa ataccggttc ctcatgatcg acaaactgcc cgtatcccgc
     2461 agccgcccga atatcatatc tcggcacgaa gacaaactcg tccgtatcga cttcattgcc
     2521 caaagtatcg taagcaaggg atttttttgg ggcttcatcc ggaaacggat taccctcccc
     2581 ggtcagcagc caatcgatac tacacccctt caactgcttg atttttttca atgtttcaga
     2641 ctttggcaga ccgccttcat tccatatcct gctgaagccc gcaatcgtca tttcaatatc
     2701 ggatgcgatt tttgcctgcc tcgcttcgct tttccataaa aaaaccagtc tgtctttaaa
     2761 ggtttccata aatatccttc cactcccctt cattcaatca tctaaagtaa acatgaaata
     2821 tattttacct tagctatcca aaaaacggaa caattatgat aaactaaatt taaattatga
     2881 tttttcttac ttaatacata atttttcaga tttttccata ggtttaatcc tgaattgaca
     2941 gaaaaaggat agaaaaatga agaaaaccgg caaatatctt atctatactg cggcatttac
     3001 cgcattctgc tttgccttcc aagaaaaccg ttctgaagcc aaacagcccg acatcacttt
     3061 atccgcatcc ctgtgcgaac aattcaacat actgaacgcc aaagatatgg atgcagaaca
     3121 agtctccctt tccaaagaat gcgacatcat cgagtcttca cacgactggg aaaaagagta
     3181 cggcaacttg aacgaacagg aaatgctcgc cggcatcgtc tacgaataaa cctgcctgcc
     3241 atttgaaata ttatgcttga atgcattgga gccaaatgta ttaaatcaaa tataaaacca
     3301 atatattcat aaagttatat acttatagcc atgctgtagc ttgaaacagc ccgccgcccg
     3361 ccctatttac agcccatcgg gacaaaatgt tccaacatat ttctcaaaat aagcaaaatc
     3421 aaataggatt atccacatgg caaaagtaat cggtatcgac ttaggtacaa ccaactcttg
     3481 tttggccatt tccgaaaacg gtcaaaccaa agttatcgaa aacgcagaag gcgcacgcac
     3541 cacgccgtcc gttatcgctt atttggacgg cggcgaaatc ctcgtcggcg cgcctgccaa
     3601 acgccaagcg gtaaccaacg ccaaaaacac tatttacgcc gccaaacgtt tgatcggcca
     3661 caaatttgaa gacaaagaag tccaacgcga catcgaatct atgcctttcg aaatcatcaa
     3721 agccaacaac ggcgacgcat gggtaaaagc acaaggcaaa gagctgtctc ctcctcaaat
     3781 ttccgcagaa gtcctgcgta aaatgaaaga agccgccgaa gcttacttgg gcgaaaaagt
     3841 aaccgaagcc gtgattaccg ttccggccta cttcaacgac agccaacgtc aagccaccaa
     3901 agacgcaggc cgtatcgccg gtttggacgt aaaacgcatc atcaacgagc cgaccgcagc
     3961 cgctttggca ttcggtatgg acaaaggcga caacaaagac cgcaaagtag ccgtatatga
     4021 cttgggcggc ggtactttcg atatttccat catcgaaatc gccaacctcg acggcgacaa
     4081 acaatttgaa gtattggcta ccaacggcga tactttcttg ggcggtgaag acttcgacca
     4141 acgcttgatc gactacatca ttgacgagtt taaaaaagaa caaggcattg atttgaaaca
     4201 agacgtcatg gctctgcaac gtctgaaaga agctgccgaa aaagccaaaa tcgaattgtc
     4261 cagcggccag caaaccgaaa tcaacctgcc gtacatcacc atggacgcaa ccggcccgaa
     4321 acacttggcg atgaaaatta cccgcgccaa attcgaaagc ctagttgaag acctgattgc
     4381 ccgctctatc gagccttgcc gcaccgcatt gaaagatgcc ggtctgagca ctggcgacat
     4441 cgacgacgtg attttggtcg gtggtcaaag ccgtatgccg aaagtacaag aagccgttaa
     4501 agacttcttc ggcaaagagc cgcgcaaaga cgtgaaccct gacgaagctg tcgccgtagg
     4561 cgcagcgatt caaggcgaag tgttaagcgg cggccgcagc gacgtattgc tgctggacgt
     4621 aacccctctg tccttgggta tcgaaaccat gggcggcgtg atgaccaagc tgattcaaaa
     4681 gaacaccacc atcccgacca aagcgtcgca agtgttctct accgccgaag acaaccaaag
     4741 cgcagtaacc atccacgtac tgcaaggcga acgcgaacgc gcttctgcca acaaatcttt
     4801 gggtcagttc aacttgggcg acatcgcacc cgcaccgcgc ggtatgccgc aaatcgaagt
     4861 caccttcgat attgacgcca acggtatcct gcacgtttcc gccaaagaca aaggcactgg
     4921 caaagcagcc aacatcacca tccaaggttc ttcaggtttg agcgaagaag aaatcgaacg
     4981 catggtgaaa gatgccgaag ccaatgccga ggaagataaa aaactgactg aattggtcgc
     5041 ttcccgcaac caagccgaag ccctgattca ctctgtgaaa aaatctttgg ccgactacgg
     5101 cgacaaactc gacgcagccg agaaagaaaa aatcgaagcc gcgctgaaag aagccgaaga
     5161 agcagttaaa ggcgacgaca aagccgctat cgatgccaaa accgaggcgc tgggcgcagc
     5221 cagccaaaaa ctgggggaaa tggtttacgc tcaagcacaa gctgaagccc aagcaggcga
     5281 aagcgaacaa gccaatgctt ctgcaaagaa agacgatgat gtcgtagatg ccgactttga
     5341 agaagtaaaa gacgacaaaa aataattagg gcgtgtccct aatttgaata agcccaaaag
     5401 agccctattt taaccctata ttccaac
//
LOCUS       DAWHXZ010000144         5400 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000144 DAWHXZ010000000
VERSION     DAWHXZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.144"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1026
                     /gene="gshA"
                     /locus_tag="WIE35_001568"
     CDS             <1..1026
                     /gene="gshA"
                     /locus_tag="WIE35_001568"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ4154461.1"
                     /translation="FYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKIL
                     LEPLLRTRDRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTN
                     HFGAYNQVTAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAK
                     YDESGITDKPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVI
                     VQEGIYTYETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSI
                     PTGNGDNSQEAPEACKRVFEQWYSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAAS
                     IELEETA"
     gene            1149..1826
                     /gene="radC"
                     /locus_tag="WIE35_001569"
     CDS             1149..1826
                     /gene="radC"
                     /locus_tag="WIE35_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ4154462.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            1899..2624
                     /locus_tag="WIE35_001570"
     CDS             1899..2624
                     /locus_tag="WIE35_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ4154463.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            2777..4057
                     /locus_tag="WIE35_001571"
     CDS             2777..4057
                     /locus_tag="WIE35_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ4154464.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHASYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNSRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            4062..5240
                     /gene="hflX"
                     /locus_tag="WIE35_001572"
     CDS             4062..5240
                     /gene="hflX"
                     /locus_tag="WIE35_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ4154465.1"
                     /translation="MTGRTGGNGSTQAQPERVMLVGVMLDKDGTGSSAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            5237..>5400
                     /locus_tag="WIE35_001573"
     CDS             5237..>5400
                     /locus_tag="WIE35_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HEZ4154466.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTA"
BASE COUNT         1356 a         1493 c         1493 g         1058 t
ORIGIN      
        1 ttttacctgc aaaacattta cgccctcggc gagattttgc gttcggcagg gtatgaagtg
       61 cgcttgggca gcctgaatcc ggaagtaacc gaaccgaccg aatttgaaac tgcattgggc
      121 gacaaaatcc tgttggaacc tttattgcgt acccgcgacc gcgtccatct tgcagacggc
      181 ttttcgcctt gcgtggtttt gttgaacaac gatttgtctg ccggcattcc cgacatcctc
      241 aaaggcatca gccaaaccgt tttgccgccg ttgcacggcg gttggacgac gcgccgcaaa
      301 acaaatcatt tcggcgcgta caaccaagtt accgccgaat ttgccaagtt aatcggcatc
      361 gacgaatggc aaattaaccc ttattttgaa aaaatcggcg gtttggactt ccaagggcgt
      421 gaaggcgaag acgcgttggc ggaagcggta gaacgtgtgc tggcgaaaat tcaagccaaa
      481 tacgacgaat cgggcattac cgacaaacct ttcgtcatcg tcaaagccga tgccggcact
      541 tacggcatgg gcgtgatgag cgtcaaatcc gccgacgaag tgcgcggatt gaaccgtaaa
      601 aaccgcaata aaatggcgaa agtcaaagaa ggcttggaag tcagcgaagt gattgtccaa
      661 gaagggattt atacttatga aaccttaaac ggcgcggtgt gcgaacccgt cgtgtatatg
      721 atggaccgct tcgtcatcgg cggctttttc cgcgtacacg aagggcgcgg tgcggacgaa
      781 aacctcaacg ccggcggtat ggtgtttgtt ccgctgtcta acagcattcc taccggtaac
      841 ggcgataatt cccaagaagc gcccgaagcc tgcaagcgcg tattcgaaca atggtactcg
      901 ctgggtatgc cgcgctctga aaaagactgc gacgtggaca acgaacacaa ccgcctctac
      961 gtttacggcg taatggcacg cctgtcgctt ctggcggctt caatcgagtt ggaagaaacg
     1021 gcgtaagact gttttgaaat acagatgccg tctgaagcgg aaatccggtt cagacggcat
     1081 ttcggatatt tggcgtgtgg gaacatctgt ttcagacggc atctcagact atttaaaaaa
     1141 gggaaaacat gagcatcaag caatggccgg aaggcgaaag acccagggaa aagctgttgg
     1201 aacgcggggc ggcggcgttg agtgatgccg aacttttggc aatcctgctg cgcgtcggca
     1261 cgcgcggaat gagtgcggtc gatttggcgc gttatttgct gcaggagttc ggcagtttgg
     1321 ggaggctgat gagcgcggag gtcggcaaac tgtcggcata caaagggatg gggacggcaa
     1381 gtttcacaca gtttgccgtg gtcagggaaa tcgggcggcg gatattggcg gaagaattgc
     1441 aggagagcat cgtcctgtcc gatccggata cggtggccga ttatttacgc tttcatttgg
     1501 ggcaggaaaa agtcgaagtc agcgtcgcgc tgctgctgaa ccgccaaaac caactgattg
     1561 cggtcagaga gctgtcgcgc ggtacggttg cggaaaacac gatttacatc cgtgaaattg
     1621 tcaaactggc attggacgaa tatgccgaca gcctgattat cgcgcacaac catccgggcg
     1681 gctcgcccga accttcgcag gaagacatca tgttcacaag gcggctggca caggcaatgt
     1741 cgctggtcga tgtgtcgctg ctcgaccatt ttatcgtaac ggcgcaaacc gtccgttcgt
     1801 tcaggcagct cgggctgatg ccctgacacc ctgttttaca tgcggcggct ctgataaaat
     1861 agccgcttca accgtattcg acagatatcg ttaagttaat ggaaacacaa aacaaaccta
     1921 ccgttaccga cattgaccgc cccatactcg tcccgcccgg cggacataaa aaagtcttgc
     1981 tgcattcctg ctgcgccccg tgcagcggcg aagtgatgga ggcgatgctt gcctccggca
     2041 tcgactacac catttatttt tacaatccca atatccatcc gcacaaagag tatatgctcc
     2101 gaaaagagga aaacgtgcgc tttgcggaaa agttcggcat tcctttcatc gataaagacg
     2161 acgactacga aaacgaccgc aaagaatggt ttgccaaagc caaaggcatg gagtttgagc
     2221 cggaacgcgg catccgctgc accatgtgtt tcgatatgcg ttttgaaaag gcggcgcaat
     2281 acgcgcatga acacgggttc cccgtcttta ccagttcgct gggcatttca cgctggaaaa
     2341 atatggcgca aatcaacgac tgcggacacc gcgccgccgc gccttacgat gatgtggtgt
     2401 attgggattt caactggcgc aaaggcggcg gcagcgcgcg catgattgaa atcagcaaac
     2461 gtgaaaactt ctaccagcag gaatattgcg gttgcgccta ttccttgcgc gattccaatg
     2521 cccaccgcaa atcgcagggc agaatcccca tcaaactcgg cgtgctgtat tacggcgacg
     2581 aatcgacaca atatgaacct gcacccatcc gggtggacaa ataaacatcc gatgccgtct
     2641 gaaggttcag acggcatcag gttcggcatc ggcacgggga aaggtttgcc ggtttggcaa
     2701 tctgcaatcg gaaaccgcat tggcaagttt gccgttttga taaaacaccc cgttgccgcg
     2761 tcgggaggac ggcattatga aatccctttt tattcggctg ctcctgttgg gttcggcggc
     2821 aggcgttttc taccataccc aaaaccaatc cctgcccgcg ggcgaacttg tctatccgtc
     2881 cgcaccgcaa atcagggacg gcggcgatgc gctgcactac ctcaaccgca tccgagccca
     2941 aatcggtttg cacaagctgg cacacgcgcc ggttttggaa aactccgccc gcaggcacgc
     3001 aagctacctc acgctcaatc ccgaagacgg acacggcgaa caccatcccg acaatccgca
     3061 ctacaccgca caaaagctga ccgaacgcac acgccttgcc gggtatctct acaacggcgt
     3121 gcatgaaaac atcagcacgg aagaggaagc cgccgaatcg tccgacagcg acatccgcac
     3181 gcagcaacgc caagtggacg gattaatgag cgcaatctac caccgccttt ccctacttga
     3241 ccgccatacg gatgaggcag gagcggcatt tgtgcgcgaa aacggtaaaa ccgttctcgt
     3301 attcaatcag ggcaacagca ggtttgagcg gcattgcgcc caaggcagaa atcagccgga
     3361 agcaggacgg aaatattacc gcaacgcctg ccataacggt gcggtcgtgt acaccgacga
     3421 agccatgccc gcacaggagc tgctctatac agcctatccc gtcggcagcg gcgcactgcc
     3481 ttatttccac ggcgagcgtc cagaccccgt gccggaatat gaaatcacgg gcaatcctgc
     3541 cagcattgat ttttccgagg cggcaggcaa aattacgatg aaaagtttca agctgtatca
     3601 gggtaaaaac gaaatccgcc ccgtcagggt tttaaccgcc ggcaacgacc ccaacggcag
     3661 gctgaccgcg taccaattcg cgctttttcc gctcaagcct ttggaatacg gcacgcttta
     3721 tacggcggta ttcgactatg tccgcaacgg acggcgcgcg caggcgaaat ggcagtttag
     3781 aacccgaaaa cccgattacc cttattttga ggtaaacggc ggcgagacac ttgcggttag
     3841 aaaaggcgaa aaatatttca tccactggcg cggacgctgg tgtttggaag cgtgtacccg
     3901 ttatacctac cggcagcgac ccggcagccg cctgtccata ggaaggcacg aggcgggcgg
     3961 catcgtcttc agcgttgacg gaatggcggg cagccgcatc acgcttgcac cggaaggaga
     4021 aacggaacga ggcgtaaccc tttatttaca ggattgaata catgacaggc agaacaggcg
     4081 gcaacggcag tacccaagcg caacccgaac gcgtcatgct ggtgggcgta atgttggaca
     4141 aagatggtac gggcagcagt gccgcccgtc tgaacggttt tcagacggca ttggcggaag
     4201 ctgtcgagct ggtcaaagcg gcgggcggcg attccgtgcg cgtggagact gccaaacgcg
     4261 accgtccgca caccgcgctg tttgtcggca cgggcaaggc ggcggagctg tcagaagcag
     4321 ttgccgcaga cggcatcgat ttggtcgtat tcaaccacga actcacgccc acgcaggaac
     4381 gcaaccttga aaaagaactg aaatgccgcg tattggacag ggtagggctg attctggcga
     4441 ttttcgctcg ccgcgcccgc acgcaggaag gcaggctgca agtcgagttg gcgcaattga
     4501 gccatttggc gggacgcttg atacgcggtt acggccatct gcagagccag cgcggcggta
     4561 tcggcatgaa aggccccggc gaaaccaaac tggaaaccga ccgccgattg atcgcccatc
     4621 ggatcaatgc cttgaaaaaa cagcttgcca acctcaaaaa acagcgcgcc ctgcgccgca
     4681 agtcccgcga atcgggcaca atcaaaacgt ttgcgctggt cggctatacc aatgtcggca
     4741 aatccagcct gttcaaccgg ctgaccaagt cgggcatata tgcgaaagac cagcttttcg
     4801 ccacactcga cacgacggcg cggcggctgt acatcagtcc cgaatgcagc attatcctga
     4861 ccgataccgt cggattcgtc agcgatctgc cgcacaaact gatttccgcc ttttccgcca
     4921 cgctggaaga aaccgcgcaa gccgatgtgc tgctgcacgt cgtcgatgcc gccgctccga
     4981 acagcggaca gcagattgaa gacgtggaaa acgtactgca agaaatccat gccggcgata
     5041 ttccgtgcat caaggtgtac aacaaaaccg acctgctgcc gtctgaagaa caaaacacgg
     5101 gcatatggcg cgacgctgcg ggaaaaattg ccgccgtccg catttccgtt gctgaaaata
     5161 ccggtataga cgcactgcgc gaagccattg ccgagtcttg tgccgccgca ccaaacacag
     5221 acgaaaccga aatgccatga aaaaaacctg tttccactgc ggtctggatg ttcccgaaca
     5281 cctccacctg actgtccgtt acgaaaacga agaccgcgaa acctgctgcg ccggctgtca
     5341 ggcggtcgca caaagcatta ttgacgcggg cttgggcagt tattacaaac aacgcaccgc
//
LOCUS       DAWHXZ010000145         5354 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000145 DAWHXZ010000000
VERSION     DAWHXZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.145"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(103..2934)
                     /gene="lbpA"
                     /locus_tag="WIE35_001574"
                     /gene_synonym="tbpA"
     CDS             complement(103..2934)
                     /gene="lbpA"
                     /locus_tag="WIE35_001574"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ4154467.1"
                     /translation="MNKKHGFQLTLTALAVAAAFPSYAANPETATPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTYDIQREKTEPFFSVEGERESK
                     PVAKLAGYGKYLNNQLNRWVKERIEKNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEADKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKKLVRGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDDKNQDKYLLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADH
                     KTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2931..5177)
                     /locus_tag="WIE35_001575"
     CDS             complement(2931..5177)
                     /locus_tag="WIE35_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ4154468.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAPTLSDSKS
                     SNPADKPAPAPAEPSVEITPVNRPAVGAAMRLPRRNTAFHREDGTVIPDSKQAEEQLS
                     FKEGDVLFLYGSKENKLQQLKSEIHKRDPDVEIRTSENENKKYDYKFVDAGYVYVKGK
                     DEIKWTSDHKQFSNRLGYDGFVYYSGERPSQSLPSAGTVEYSGNWQYMTDVKRHRAGK
                     AVGIDNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDKKILKGELIKNQYVRD
                     RNGPYNPLTIYDITATLDGNRFTGSAKVNTKLKTSHADKEYLFFHTDADQRLEGGFFG
                     DNGEELAGRFISNDKSVFGVFAGKQKTETANASDTNPALPSGKHTKILDSLKISVDEA
                     ADKKARPFEVSPMPDFGHPDKLLVEGREIPLVSQEKTIKLADGREMTVRACCDFLTYV
                     KLGRIKTDRPAVKPKVQDEEGDEEDTGVDSVEEGEIGIGDEDEGTEDEAVEDGEESEE
                     EISEDDNGEDEEEIAEDEGEEEPEEESPEEGNGSSGSIPPAPEASKGRDIDLFLKGIR
                     TAETDIPHSGTAHYTGTWEARIGTPIQWDNQADKTAKAKFTVDFGGKSISGTLTEENG
                     VEPAFYIEKGVIDGNGFHATAHTRDTGINLSGTGSTNPQRFKASNLRVEGGFYGPQAE
                     ELGGIIFNNDGKSLGITEGTENKVEADVEAEAEAEVEAGVEQLKPEVKPQFGVVFGAK
                     KDMQEVEK"
BASE COUNT         1162 a         1442 c         1232 g         1518 t
ORIGIN      
        1 ggaaatcttg tttatattga atcaaaaaaa cctgcacctt aatcagttgg cggtttagtc
       61 cgacttttgg ggtgcagatc aagctttcag acggtatttc ctttaaaact tcatttcgag
      121 cgcgaggctg aagttcctgc ccggtgcggc ataccttcca tagttgctgt cgccgccgtg
      181 ccggtttgcc gtgctttccg cggtctggcg caaggattcc caagtaacgt agcggtagtt
      241 gccgatattg tagatagccg ccctcaaggt cagccgtttt ttcagattca gataggcgga
      301 aacgtctgcc gtcgaccaag aagacgacgc tctttttgtc gaatatcgtt tttgatcgcc
      361 tgccagataa gcaagctcgt cagggttttt ccctttggaa taggtcagca taatgtttgc
      421 gccccatttc ccctcgggct ggtcgtatcc gaaccccaaa acataacgcg acggctgtac
      481 cgcatccaaa gcatagctgc ggagggacag tcccggccgg ttggataccg atttcggttt
      541 gatacggttg tacgccaatg tggtatacaa accttcgggc agtttgccgt acacaccgtt
      601 ccagtcgatt tttcccaata tattaacgcc ttggagcgac atattttggg cattgtaata
      661 atcgcgtata tcaatctctg tcaattgtcc tgcctgattc ggcaatttgg ttttgtgatc
      721 ggcaacggca atcatatcgg tatagcggtt gcggaagctg ctgatttcca aaaagccgaa
      781 atcgcccttc cactgcaaac cgatttcgcg gttggctgcc ttttccgatt tcagggcggg
      841 acgctgccag cctttcggat aatcgtgata aatgtctatc ccgaaaagtt cttggaatga
      901 gggcgttctg aagccgctgg aggcgcggta agacacggaa aaatgccggt tcggtttgaa
      961 cacgatgccg ctgttccacg aacggtcaac ataccgcccg ctgcggacga gttcttccga
     1021 cgtggtgaag tttttccggt cgtacctgcc gcccaagctg aaatcaaaat atttgccgat
     1081 tgaaaaacgg tcgttcaaag aaatgtggat attgctgccg ttgatttttc ttggcacgca
     1141 tttgcgggaa cgcaaagttt cgatgtagcc gcagaccgac ccttcgacga cttcgggctt
     1201 acccaaaaga tacttatctt gatttttatc atcgaatccc gtggattccg aaatccttgc
     1261 cgcattgtgg gaaagctgtt cggggcggga gatcgctttg gaagcatcgt aaccgaagcc
     1321 caaagtcaga tggtgtttcg tccatttgtt tttcagcgat ttctcaaacg aggcattcaa
     1381 aacattgtgc tgttcgcggt agtggaaacg gtcgctgctg tcgtaggaat acggtttgtc
     1441 cgccgacgcg cggcaggatt tgtccacagc aggatacacg gcacaattca gcttcagcgt
     1501 gttgttgtcg gttgccacgc cctgtttgtc aaacgacaac accgccttgt ccgcccaatt
     1561 gtcagaatac gcttcgtttt cataacggta cagcaaaccc atacggcggc ggcggtgatg
     1621 ttcgtcaata aatttggtgc gggaatattt caaacctatg cccctgacca actttttatc
     1681 gcccttccac tcttctatat tcggcacaaa atacaagccg tcgcggaaat cgtcgccgtc
     1741 gtacaccccg ctcttgtctc taaacttgtc cgcctcgtcc gtaccgtaat actgtttttc
     1801 cgtcatatcg cggatgtcgt aacgctgttt ggtatcctca aacacgccgc cgacataatg
     1861 cctgccgccg aagcggtagc ccagcttggc aagccaagag ccgctgcggt aatccatcgg
     1921 atcgggcaat atcctgccgc cgcccgtgta agcttgggcg gacaggtttt cgtggcgcgc
     1981 ctgcgcctcc cgcacctgcg cctcttcttc agcacttaaa ggctgatttt tttcaatacg
     2041 ttcttttacc cagcggttga gctggttgtt caaatatttc ccgtagcccg ccaattttgc
     2101 cacgggcttg gattcacgct cgccctctac tgagaaaaat ggctctgttt tctcgcgttg
     2161 aatatcgtat gtctggcgga acgcgtccaa acggtttatg ccgtatgcca ccccgtccgc
     2221 aatatcgccg tgcgggcgcg tttcccgccc ctgacgttcg gttcggatta acagcccttc
     2281 ccaaccgtct ttgctgaacc ccgcgccgag cgacttcata aattggcggt ttttactgcc
     2341 gtaggcggtt tttgcctgta tcccccaact tttgccgtct gaaatcaggt ctgccgcctc
     2401 tttggtgcgg aaggcgaccg cgccgccgag tgcgccgctg ccgtgatcgg acgaaccggc
     2461 acctttgtcg atttccaccg tgctgatgtt ttcatattcg atttcgttga ttgcaccgct
     2521 gccgccgcgt ccgccgtatc cgctcaacga tccctgcacg gtaaacgcct gtatttgggc
     2581 aacgccgtca accgaaaccg ccacacggtt tttatccacg ccgcgtatcg agtagccgcc
     2641 gctcgcgccg ttgccctgtt cgacaaccgc cacgcccgga tcgtagcgcg tcaggtcgcg
     2701 gataccgagt acctgttctt tgttcaaagt ttccgacgtt ttgacgattt tgcccaaacc
     2761 ggtcgcctct ttcgatcgcc gtcccacttt ggcggcacgg acggtaacct ctttcaggga
     2821 ttgggtctgc gccgcatcag gtgtcgccgt ttcggggttt gccgcatatg atgggaaagc
     2881 ggctgcaaca gccaaggcag tcagagtaag ctgaaaaccg tgtttcttat tcatttttcc
     2941 acctcctgca tatctttctt cgcaccgaat accacgccga attggggttt aacttcaggt
     3001 tttaactgtt caacaccagc ttcaacttca gcttcagctt cagcttcaac atcagcttca
     3061 actttatttt cagtaccttc agttatacca agagatttcc catcattatt gaaaataata
     3121 ccgcccaatt cctccgcctg cgggccgtaa aatcctcctt ctacacgaag attactggct
     3181 ttgaaacgtt gaggattagt cgaaccagtt cccgaaagat tgatgccggt atcccgagtg
     3241 tgtgctgtcg cgtggaaacc gttgccatca atcacacctt tttcaatata gaaagcaggt
     3301 tctacaccgt tttcctccgt cagcgttcca gaaatcgatt tcccgccgaa atcaacggta
     3361 aattttgctt ttgcggtttt atccgcctga ttgtcccatt gaatgggtgt gccgatacgc
     3421 gcttcccaag tgccggtata atgcgccgtt ccgctatgcg gaatatccgt ttccgccgtg
     3481 cggatacctt tcaggaaaag gtcgatgtcc ctgcctttag aggcttccgg ggcaggcggg
     3541 atgctgcctg aactgccgtt tccttcttcc ggcgattctt cttcgggttc ttcttcgcct
     3601 tcgtcttcgg cgatttcttc ttcgtcttca ccgttatcat cttcggaaat ttcttcttcg
     3661 ctttcttcgc cgtcttctac ggcttcgtct tccgtgcctt cgtcttcatc gccgattccg
     3721 atttcgcctt cttcgacgct atcaacgcct gtatcctctt cgtccccctc ttcatcctgc
     3781 accttcggtt tgacggcggg acgatcggtt tttatccgtc cgagtttcac ataggtcaaa
     3841 aagtcgcaac aagcacggac ggtcatttcc ctgccgtcgg caagcttgat ggttttctct
     3901 tggctaacca aaggaatttc acgcccttcg acaagaagct tgtcgggatg accaaaatcg
     3961 ggcatagggg aaacctcaaa cggacgggct tttttatcag ccgcctcgtc aacggaaatt
     4021 tttagagaat ccaagatttt ggtgtgtttt ccagacggca gggcaggatt tgtatctgat
     4081 gcgtttgctg tctctgtttt ttgtttgcct gcgaatacgc cgaatacgct tttgtcgttg
     4141 ctgataaacc gtccggcaag ctcttctccg ttatcaccga aaaaaccgcc ctcaagccgc
     4201 tgatcggcat cggtatggaa aaacaaatat tctttatcag cgtggctcgt cttcaacttg
     4261 gtgttaactt tggcactgcc ggtaaagcgg ttgccgtcca atgttgcagt aatgtcgtaa
     4321 atggtcagcg gattataggg accatttcta tctcgcacat actgattttt aatcagctcg
     4381 ccctttagga tttttttgtc gaaatcaacc gtatatttgg caggatgctt ttccctttcg
     4441 tcgacatcct tagccgcata agaagttgca ccaacatcgt taccataaaa tgtgatataa
     4501 cccaaattgt caatgccaac cgccttacct gctcgatgac gtttgacatc ggtcatatat
     4561 tgccagttac cagaatattc caccgttccc gcactcggta aagattggga aggacgttct
     4621 ccggaataat atacaaaacc gtcataacct aagcggttgg aaaactgctt gtgatctgaa
     4681 gtccacttaa tttcatcttt tccctttaca tatacataac ctgcatctac aaatttataa
     4741 tcatattttt tattttcatt ttctgatgtc ctaatttcta catcgggatc acgtttatga
     4801 atttcgcttt taagttgttg aagtttattt tcttttgaac cgtataaaaa cagaacatca
     4861 ccttctttaa acgacagctg ttcttctgct tgtttgctat cgggaattac cgtgccatct
     4921 tcacgatgaa aagcagtatt ccgccttggc agccgcattg ccgcaccgac ggcgggccgg
     4981 ttgaccggcg tgatttctac cgaaggctcg gcaggagctg gagcaggctt atccgcagga
     5041 ttggaagatt tggaatctga cagagttggc gcggtcggcg ttgattcgac aacaggctgt
     5101 acgccgaaat tgccgccgat acaagatgcc aaaagtaagg gcaacaagac aatgccgcca
     5161 taattcggtt tacacatccc tacttttcct cctatttgat taataataat tatcattata
     5221 ttaatatgta cagataatat caagccgttt ttatagtgaa ttaacaaaaa tccggacaag
     5281 gcgacgagcc gcagacagta cagatacatc ccgtcattcc cacgaacctg catcccgtca
     5341 ttcccacgaa ccta
//
LOCUS       DAWHXZ010000146         5354 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000146 DAWHXZ010000000
VERSION     DAWHXZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.146"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            153..1058
                     /locus_tag="WIE35_001576"
     CDS             153..1058
                     /locus_tag="WIE35_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ4154469.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGPYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFNERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSEP"
     gene            1104..1253
                     /locus_tag="WIE35_001577"
     CDS             1104..1253
                     /locus_tag="WIE35_001577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154470.1"
                     /translation="MNWQAVKTGKTKHASNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(1472..2491)
                     /gene="lepB"
                     /locus_tag="WIE35_001578"
     CDS             complement(1472..2491)
                     /gene="lepB"
                     /locus_tag="WIE35_001578"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ4154471.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFAAVFLIFVVLCGTAWAVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFRSDLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRS
                     GTAIR"
     gene            complement(2636..4429)
                     /gene="lepA"
                     /locus_tag="WIE35_001579"
     CDS             complement(2636..4429)
                     /gene="lepA"
                     /locus_tag="WIE35_001579"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ4154472.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(4571..5272)
                     /locus_tag="WIE35_001580"
     CDS             complement(4571..5272)
                     /locus_tag="WIE35_001580"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ4154473.1"
                     /translation="MSLKTVAVIGAMEQEIKLLREMMENIKAVSFGRFSAYEGELVGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPDCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPARFASDGILIEAAKRAARTFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AANSAKMVAEIVKSL"
BASE COUNT         1175 a         1363 c         1447 g         1369 t
ORIGIN      
        1 gggttttatc tggcaggacg gttttttgcc ccaacggaaa atagtcggcc tgcccgtaaa
       61 atcagccgtt tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat
      121 ggcggcattc ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg
      181 gtaacggcaa aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg
      241 gcagtctgat tgtcaggtcc gtccccgtcg gcccgtatgc aatcgcattt tggcggttgc
      301 tgatttcggt gttcgtattt tggtttttgg cacggttttt cgggcaaaaa ttcccaaaaa
      361 acaggaaaac cgtccgatat gccctgacgg cgggcgtgtt cctcgctttc gatttggcgt
      421 tgtggcacga aagcatacac gcggtcgggc cgggtatttc caccctgctc aacagcctgc
      481 aaatcttttt cttgtcggca atcggtgttt tcttttttaa cgaacgtttg agcgggctga
      541 aaaaggcagg cttaatatcg gcagttgccg gcgtggcgat gattgccggt gcggaattcg
      601 gctacaacgg taatgcggtt tggggattcg ccagcggttt ggtatcggga ctgatgctcg
      661 ccctgtcgat ggtgttcgtc cgcaaaaccc atgaaatcga gccggtggcg cttttccctt
      721 caatgatgat tttgagtttg ggcggcgcgg tatcgctggt tgttccggca ttgctgatgg
      781 atggcggcgt gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg
      841 tggtgatgca gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt
      901 cgctgacggg gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg
      961 ggttgggcaa accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa
     1021 tttacctcgg ttcgctgaaa cagcagtccg agccttgact ccgtccggca tcagattttc
     1081 tgccgtggca aacctgcgta cgattgaact ggcaggcagt caaaaccggc aagaccaaac
     1141 acgccagcaa cggcgcggag cggaaaatgc cgtctgaagc cctttcagac ggcatcgggg
     1201 cgggcagaat caaaaaagcc gcctccgctg gaatggcaat gcaactaaaa taatgtccgt
     1261 atttccatat gccgcccaca ccgtcagtcg gacgaaatgc cctttattca atgggatggg
     1321 tttccacaca aacggaaata tagtggatta aatttaatcc actatattat ccccgaggct
     1381 gccggcaggg aaacaaggcg tttgatgata aaaatgccgt ctgaaacagg gttcggatgg
     1441 catttttgat gttccggcgt aattttccgg cctaacggat tgccgtaccg gacctgccga
     1501 aatcgccgaa gttcatcaaa atgaacattg ccttgccgac aaccagcttg tcatccacaa
     1561 atccccagta gcgcgaatcg gcactgttgt cgcggttgtc gcccatagcg aaatagcgtc
     1621 cttcgggaac tttgcacacg aaaccgctgc cgtcgtcggc atattggcag tgttccaaac
     1681 cgctttgctc tatggaatat ccgttttcag acataatatc ggaggtatat ttgcccaata
     1741 cgggcaggga aacggcaggc tgtccttctt ttttcagaat attgaaggat ttgccgtcca
     1801 gatcgctgcg gaacatatcc gtgttgtgga tttcggaagg gtcggtgtcg tcgggataac
     1861 ggtatgtgcc gttaggaata tcggaagtgg gtttgccatt taccgtcaaa atcttatccc
     1921 gatattcgac cacatcgccc ggaatgccga caatacgctt gatgtaggtc atctccggct
     1981 gcagaggata attaaaaaca acgacatcgc ctcgttcgat tttgcctgta ggaataaata
     2041 tattgtttaa aacgggtacg cgcagaccgt aggaaaattt gccgaccaaa atgaaatcgc
     2101 ccttaatcag gcccgggcgc atcgagctgg acgggatttg gaacggttcg gcaataaacg
     2161 accggatgag gaacaatacc aaaacggtag ggaagaaact gccgaaataa tcgccgaagt
     2221 ggctgctttc cgagatttcg ggatgagtct tcaggcggta tttgtatacc gcccaagccg
     2281 taccgcacaa tacaacgaaa atcaggaaaa cggcggcaaa gctcataaac agggacaaag
     2341 cggcaaacac gccgaccgcc gtcaggatat aggcgtattc gaggccggaa ctccattcac
     2401 cgttttcctg ccgcttcttg tcgcttttga aataaaggat gatgccggca agcagcgcgg
     2461 cagccgcgcc cgacattagc attgtgttca ttgttgttcc tcaacggtta aaaacccgcc
     2521 cggccgtgca accgttttaa ggcggcaaat tgcaaaattt gtttgcgggc gcgtgcccct
     2581 gaaatcaggg cggtttgaga ggtgttcccg acgcgccgcc ctatgtgccg aaatattatt
     2641 tgtcgctcac ctgcaaaatc gccaagaacg cgctttgcgg gatttccaca ttgcccactt
     2701 gtttcatacg gcgtttacct gccttttgtt tttcaagcag ttttttctta cgcgtaatat
     2761 cgccgccgta acatttcgcc aagacgtttt tacgcagtgc tttgacgttt tcgcgggcga
     2821 taatctggct gccgatggcg gcttggacgg caatgtcgaa catttggcgc ggaatcagct
     2881 cgcgcatttt cgatgccagc tcgcggcctc ggtgaaccgc gctttgacgg tgcacaatca
     2941 ggcttaaggc atcgactttt tcgccgttga ccatgatatc cagcttaatc aaatcagacg
     3001 gttggaactc tttgaaatgg tagtccaacg aagcataacc gcgcgaagtg gatttgagtt
     3061 tgtcgaagaa gtccatgacg acttcgttca tgggcaaatc gtaagtcagc atgacttggc
     3121 ggcccatata ttgcatattg acctgaacgc cgcgcttttg gttacacaaa gtcatgacgt
     3181 tgccgacgta ttcctgcggc acgaggatgg tcgcggtaat aatcggctcg agtatggttt
     3241 cgatgctgcc gatgtcgggc agtttggacg ggttttcaac ttcgattttc tcaccgtttt
     3301 tcaacacgac ttcgtaaacc accgtcggcg cggtggtaat caagtccatg tcgaactcgc
     3361 gctccaaacg ttcctgcacg atttccaagt gcagcagacc caagaagccg caacggaagc
     3421 cgaagcccaa tgcctgggaa acttcaggct cgaatttcaa cgaagcgtcg ttaagctgca
     3481 atttttccaa agcgtcgcgc aaggcttcgt agtcgtggct ttccacggga taaagtccgg
     3541 cgaatacttg gctttgtact tcttggaagc cgggcagcgg ctcagtggca gggttggcaa
     3601 ccagtgtaac cgtatcgcca actttcgcct gacccaattc ttttacgcca gtaatcaaaa
     3661 agcccacttc gccggctttg agttcttgtt tttgaactga ttttggtgtg aatacgccca
     3721 gctgctcgac ctgcgtttcc gccttggtgc tcataaagcg cactttgtct ttcagtttga
     3781 tggtgccgtt tttcactcga atcagcatga ccacgccgac atagttgtca aaccacgaat
     3841 cgacgatcac ggcttgcagc ggcgcgtttt cgtcgccggt cggcgcaggg attttggcaa
     3901 cgatttcttc taaaacgtct tccacgccga tgccgctttt ggcggaacat tgcaccgcac
     3961 cgacggcatc gatgccgata atgtcttcga tttcttgttc cacgcgttcg gggtcggcgg
     4021 cgggcaggtc gattttgttt aaaacaggca caacctccac gcccaaatca atcgcggtat
     4081 agcagttcgc cacagtttgc gcttccacgc cctgcgacgc gtcaacgacc aaaagcgcgc
     4141 cttcgcaggc agagagcgaa cgagagactt cgtaagagaa gtcgacgtgt cccggcgtgt
     4201 caatcaggtt gagttgatac acctgcccgt cgcgtgcttt atagttgagc gcggcggttt
     4261 gcgctttaat ggtaatgccg cgctcttttt cgatgtccat ggaatcgagt acctgcgtac
     4321 tcatttcgcg caaatccaaa ccgccgcaat attggatgaa gcggtcggca agcgtcgatt
     4381 tgccgtggtc gatgtgggca atgatggaga aatttcggat atttttcatt gagttgtttt
     4441 taatgctgaa cggtaggttt tagaaatgcc gtctgaacaa acagcggtac gtccaaatat
     4501 cgggcgcaac gtggaaatag cccgatattc taacggaaaa ccgctgtttt ggcatgattt
     4561 gataaaggtt ttataaagat ttgacgattt ctgccaccat ttttgcggaa tttgccgccg
     4621 ccgttttcaa gaactcgtca aagctgatgt ctgccttttc gtctgccgaa tcggaaaccg
     4681 cgcggatgat gacgaaaggc gtttccaact gatgacaggt ttgggcgatt gccgccgctt
     4741 ccatttctac cgccttaact tcggggaagt gcttgcggat ttccgccacg ccttcgctgc
     4801 tgtggacgaa gcggtcgccg ctgacaatca ggccttgttc taccgccgcg ccttcaaacg
     4861 tccgcgccgc ccgttttgcc gcctcaatca aaatgccgtc tgaagcaaac cttgccggca
     4921 gttgcggcac ttgtccccaa acatagccga atgcggttac gtcgacatcg tggtgtgcgg
     4981 tttccgtacc gatgactacg tcgccgactt tcaaaccctt gcccaaaccg cccgcgctgc
     5041 cggtgttgat gacgcagtcc ggtgcgaatt gatggataag ccaagccgtt gcaaccgccg
     5101 cgttgacctt gccgatgccg ctcaatgcaa gcaccatgcg ttttcccacc aattcgcctt
     5161 cataggcgga aaatctgccg aaagagacgg ctttgatatt ttccatcatc tcgcgcaaaa
     5221 gcttgatttc ttgttccatt gcgccgataa cggctactgt tttcaaagac atattgctga
     5281 cctgttgtga atttcggata gaatgccgga ttatacacgc taacacggca ggattgagtg
     5341 ggggcggttt gtcc
//
LOCUS       DAWHXZ010000147         5338 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000147 DAWHXZ010000000
VERSION     DAWHXZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.147"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            152..2296
                     /locus_tag="WIE35_001581"
     CDS             152..2296
                     /locus_tag="WIE35_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ4154474.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGGSFDLDSVDTEAPRPAPK
                     YQDVSSEKPQAQKDQGGYGFAMRFKRRNWHPQANPKEDEIKLSENDWEATGLPGNPKN
                     LPERQKSVIEKVKTGSDSNIYSSPYLTQSNHQNGSANQPKNEVTDYKEFKYVYSGWFY
                     KHAKLEIIKENNLIKGAKSGDDGYIFYHGEKPSRQLPVSGEVTYKGVWHFVTDTKQRQ
                     KFNDILGTSKKQGDRYSGFPGDDGEEYSNKNEATLQGSQEGYGFTSNLKVDFNKKKLT
                     GELIRNNRVTNATANDKYTTQYYSLEAQVTGNRFNGTATATDKPKKDGETKEHPFVSD
                     SSSLSGGFFGPKGEELGFRFLSDDKKVAVVGSAKTKDKDANGNTEAASGGTGAAASGG
                     AAAMPSENGKLTTVLDAVELTHGGKAIKNLDNFSNAAQLVVDGIMIPLLPEASESGNT
                     NQGTNGGTAFTRKFDYTPKSDEKDTQAGTAANGDQAASNTAGDTNGKTKTYEVEVCCS
                     NLNYLKYGLLTRKTAGNTGEGGNGSPTAAAQTAQGAQSMFLQGERTDEKEIPSEQNVV
                     YRGSWYGHIANGTSWSGNASNATSGNKADFTVNFGEKKINGTLTAENRQAATFTIEGT
                     IQGNGFSGTAKTADSGFDLDQSNTTGTPKAYITDAKVQGGFYGPKAEELGGWFAYPGD
                     KQTENATVASGNGNSASSATVVFGAKRQQLVQ"
     gene            2383..5127
                     /gene="tbpA"
                     /locus_tag="WIE35_001582"
     CDS             2383..5127
                     /gene="tbpA"
                     /locus_tag="WIE35_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ4154475.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEAAGRGVQSFNRLVPVEDDNQYAHFVVEEECESRNYETCKANP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERT
                     QQTFDTRDMTVPAFLTKAVFDENKKQAGSLSGNGKYAGDHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     NLRHQDYYHQSANRAYSSKTPPKNGNQNSPNGDKSKPYWVSIGGGNVVTGQICRLGNN
                     TYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLS
                     WNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIV
                     FKGDFGNLEASWFNNAYRDLIVRGYEAQIENGKETAKGDPAYLNAQSARITGINVLGK
                     IDWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQ
                     PEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIK
                     KHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF
                     "
BASE COUNT         1534 a         1345 c         1365 g         1094 t
ORIGIN      
        1 ggtttccgtg cggacgcgtt cggattcacg actgcgcggg aatgacggga ttttggtgta
       61 ttccctaaaa aaaataaaaa aagcatttgc aaatttatta aaaataaata aaataataat
      121 ctttatcatt ctttaattga attaggttta tatgaacaat ccattggtaa atcaggctgc
      181 tatggtactg cctgtgtttt tgttgagtgc ttgtttgggc ggaggcggcg gcagtttcga
      241 tcttgattct gtcgataccg aagccccgcg tcccgcgcca aagtatcaag atgtttcttc
      301 cgaaaaaccg caagcccaaa aagaccaagg cggatacggt tttgcaatgc gcttcaagcg
      361 gcggaattgg catccgcagg caaatcctaa agaagatgag ataaaacttt ctgaaaatga
      421 ttgggaggcg acaggattgc caggcaatcc caaaaactta cctgagcgac agaaatcggt
      481 tattgaaaaa gtaaaaacag gcagcgacag caatatttat tcttccccct atctcacgca
      541 atcaaaccat caaaacggca gtgcaaacca accaaaaaac gaagtaacag attataaaga
      601 gttcaaatat gtttattccg gttggtttta caaacacgct aaactcgaaa tcataaaaga
      661 aaacaactta attaagggtg caaagagcgg cgacgacggt tatatctttt atcacggtga
      721 aaaaccttcc cgacaacttc ccgtttctgg agaagttacc tacaaaggcg tatggcattt
      781 tgtaaccgat acgaaacaga gacaaaaatt taacgatatt cttggaacct caaaaaaaca
      841 aggcgacagg tatagcggat ttccgggtga tgacggcgaa gaatattcca ataaaaatga
      901 agcgacttta caaggcagtc aagagggtta tggttttacc tcaaatttaa aagtggattt
      961 caataagaaa aaattgacgg gtgaattgat acgcaataat agagttacaa acgctactgc
     1021 taacgataaa tacaccacgc aatattacag ccttgaggct caagtaacag gcaaccgctt
     1081 caacggcacg gcaacggcaa ccgacaaacc caaaaaagac ggcgaaacca aggaacatcc
     1141 ctttgtttcc gattcgtctt ctttgagcgg cggctttttc ggcccgaagg gtgaggaatt
     1201 gggtttccgc tttttgagcg acgataaaaa agttgccgtt gtcggcagcg cgaaaaccaa
     1261 agacaaagac gcaaatggca atactgaggc ggcttcaggc ggcacaggtg cggcagcatc
     1321 gggcggtgcg gcagctatgc cgtctgaaaa cggtaagctg accacggttt tggatgcggt
     1381 cgagctgacg cacggcggca aagcaatcaa aaatctcgac aacttcagca atgccgccca
     1441 actggttgtc gacggcatta tgattccgct cctgcccgag gcttccgaaa gcgggaatac
     1501 caatcaaggt acaaacggcg gaacagcctt tacccgcaaa tttgattaca cgccgaaaag
     1561 cgatgaaaaa gacacccaag caggtacggc ggcgaatggc gatcaagccg cttcaaatac
     1621 ggcaggtgat accaatggca aaacaaaaac ctatgaagtc gaagtctgct gttccaacct
     1681 caattatctg aaatacgggt tgctgacgcg caagactgcc ggcaatacgg gggaaggcgg
     1741 caacggcagc ccaaccgccg ccgcccaaac ggcacagggt gcacaaagta tgttcctcca
     1801 aggcgagcgc accgatgaaa aagagattcc aagcgagcaa aacgtcgttt atcgggggtc
     1861 ttggtacggg catattgcca acggcacaag ctggagcggc aatgcttcca atgcaacgag
     1921 tggcaacaag gcggacttta ccgtgaattt tggcgagaaa aaaattaacg gcacgttaac
     1981 cgctgaaaac aggcaggcgg caacctttac cattgaggga acgattcagg gcaacggttt
     2041 ttccggtacg gcaaaaactg ctgactcagg ctttgatctc gatcaaagca ataccaccgg
     2101 cacgcctaag gcatatatca cagatgccaa ggtgcagggc ggtttttacg ggcctaaagc
     2161 cgaagagttg ggtggatggt ttgcctatcc gggcgataaa caaacggaaa atgcaacagt
     2221 tgcatccggc aatggaaatt cagcaagcag tgcaactgtc gtattcggtg cgaaacgcca
     2281 acagcttgtg caataagcac ggctgccgaa caatcgagaa taaggcttca gacggcatcg
     2341 ctccttccga tgccgtctga aagcgaagag tagggaaaca ctatgcaaca gcaacatttg
     2401 ttccgattaa atattttatg cctgtcttta atgactgcgc tgcccgttta tgcagaaaat
     2461 gtgcaagccg gacaagcaca ggaaaaacag ttggatacca tacaggtaaa agccaaaaaa
     2521 cagaaaaccc gccgcgataa cgaagtaacc ggtttgggca aattggtcaa aaccgccgac
     2581 accctcagca aggaacaggt actcgatatc cgcgacctga ctcgctacga ccccggcatc
     2641 gccgtcgtcg aacaggggcg cggcgcaagc tcgggctatt cgatacgcgg tatggacaaa
     2701 aaccgcgttt ccttgacggt agacggcttg gcgcaaatac agtcctacac cgctcaggcg
     2761 gcattgggcg ggacgaggac ggcgggcagc agcggcgcaa tcaatgaaat cgagtatgaa
     2821 aacgtcaagg ctgtcgaaat cagcaaaggc tcaaactcgg tcgaacaagg cagcggcgca
     2881 ttggcgggtt cggtcgcatt tcaaaccaaa accgccgacg atgttatcgg ggaaggcagg
     2941 cagtggggga ttcagagtaa aaccgcctat tccggcaaaa accgggggct tacccaatcc
     3001 atcgcgctgg cggggcgcat cggtggtgcg gaggctttgc tgatccacac gggtcggcgc
     3061 gcgggggaaa tccgcgccca cgaagccgcc ggacgcggcg ttcagagctt caacaggctg
     3121 gtgccggttg aagacgacaa ccaatacgct catttcgtgg tcgaagaaga atgcgaaagc
     3181 agaaattacg aaacgtgtaa agcgaatccg aaaaaagatg ttgtcggcaa agacgaacgt
     3241 caaacggttt ccacccgaga ctacacgggt cccaaccgct tcctcgccga tccgctttca
     3301 tacgaaagcc ggtcgtggct gttccgcccg ggttttcgtt ttgaaaacaa acggcactac
     3361 atcggcggca tactcgaacg cacgcaacaa actttcgaca cgcgcgatat gacggttccg
     3421 gcattcctga ccaaggcggt ttttgatgaa aataaaaaac aggcgggttc tttgtccggc
     3481 aacggcaaat acgcgggcga ccacaaatac ggcggactgt ttaccaacgg cgaaaatggt
     3541 gcgctggtgg gcgcggaata cggtacgggc gtgttttacg acgagacgca caccaaaagc
     3601 cgctacggtt tggaatatgt ctataccaat gccgataaag acacttgggc ggattatgcc
     3661 cgcctctctt atgaccggca gggtatcggt ttggacaatc attttcagca gacgcactgt
     3721 tctgccgacg gttcggacaa atattgccgt ccgagtgccg acaagccgtt ttcctattac
     3781 aaatccgacc gcgtgattta cggggaaagc cacaggctct tgcaggcggc atttaaaaaa
     3841 tccttcgata ccgccaaaat ccgccacaac ctgagcgtga atctcgggtt tgaccgcttt
     3901 ggctctaatc tccgccatca ggattattat catcaaagtg ccaaccgcgc ctattcgtcg
     3961 aaaacgcccc ctaaaaacgg caatcaaaac agccccaacg gcgacaagag caaaccctat
     4021 tgggtcagca taggcggggg aaatgtggtt acggggcaaa tctgccgctt gggcaacaat
     4081 acttatacgg actgcacgcc gcgcagcatc aacggcaaaa gctattacgc ggcagttcgg
     4141 gacaatgtcc gtttgggcag gtgggcggat gtcggcgcgg gcttgcgcta cgactaccgc
     4201 agcacgcatt cggacgacgg cagcgtttcc accggcacgc accgcaccct gtcctggaac
     4261 gcaggcatcg tcctcaaacc tgccgactgg ctggatttga cttaccgcac ttcaaccggc
     4321 ttccgcctgc cctcgtttgc ggaaatgtac ggctggcggg cgggtgttca aagcaaggcg
     4381 gtcaaaatcg atccggaaaa atcgttcaac aaagaagccg gcatcgtgtt taaaggcgat
     4441 ttcggcaact tggaggcaag ttggttcaac aatgcctacc gcgatttgat tgtccggggt
     4501 tatgaagcgc aaattgaaaa cggcaaagaa acagccaaag gcgacccggc ttacctcaat
     4561 gcccaaagcg cgcggattac cggcatcaat gttttgggca aaatcgattg gaacggcgta
     4621 tgggataaat tgcccgaagg ttggtattct acatttgcct ataatcgtgt ccatgtccgc
     4681 gacatcaaaa aacgcgcaga ccgcaccgat attcaatcac atctgtttga tgccatccaa
     4741 ccctcgcgct atgtcgtcgg cttgggctat gaccaaccgg aaggcaaatg gggtgtgaac
     4801 ggtatgctga cttattccaa agccaaggaa atcacagagt tgttgggcag ccgtgctttg
     4861 ctcaacggca acagccgcaa tacaaaagcc accgcgcgcc gtacccgccc ttggtatatt
     4921 gtggatgtgt ccggttatta cacgattaaa aaacacttca ccctccgtgc gggcgtgtac
     4981 aacctcctca actaccgcta tgttacttgg gaaaatgtgc ggcaaactgc cggcggcgca
     5041 gtcaaccaac acaaaaatgt cggcgtttac aaccgatatg ccgcccccgg ccgaaactac
     5101 acatttagct tggaaatgaa gttttaaacg tccaaacgcc gcaaatgccg tctgaaaggc
     5161 ttcagacggc attttttaca caattcccgc cattttccat catccccgat acaccgtaat
     5221 cctgaaaccc gtcattcccg cgcaggcggg aatccagtcc gttcggtttc ggttttttga
     5281 ggtttcgggt aacttctaaa ccgtcattcc cgcgaaggcg gaaaatcaaa aacagaaa
//
LOCUS       DAWHXZ010000148         5279 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000148 DAWHXZ010000000
VERSION     DAWHXZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.148"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            141..575
                     /locus_tag="WIE35_001583"
     CDS             141..575
                     /locus_tag="WIE35_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ4154476.1"
                     /translation="MNFALSVIMLTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(623..1966)
                     /locus_tag="WIE35_001584"
     CDS             complement(623..1966)
                     /locus_tag="WIE35_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ4154477.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1971..2624)
                     /locus_tag="WIE35_001585"
     CDS             complement(1971..2624)
                     /locus_tag="WIE35_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ4154478.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2775..3065
                     /gene="gatC"
                     /locus_tag="WIE35_001586"
     CDS             2775..3065
                     /gene="gatC"
                     /locus_tag="WIE35_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ4154479.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQNI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRDRLYIVPQVIEE"
     gene            3128..4573
                     /gene="gatA"
                     /locus_tag="WIE35_001587"
     CDS             3128..4573
                     /gene="gatA"
                     /locus_tag="WIE35_001587"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ4154480.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4570..>5279
                     /locus_tag="WIE35_001588"
     CDS             4570..>5279
                     /locus_tag="WIE35_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HEZ4154481.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFW"
BASE COUNT         1316 a         1644 c         1290 g         1029 t
ORIGIN      
        1 tttgggaatc tgttttaccc caatatataa agcaccatat taaggcggag tgtcttcccc
       61 actttgaccc gaacccggaa aagacaccgc ccaagccaat cctgatgctg ccccgacagc
      121 caaccattaa ggaaatccta atgaactttg ctttatccgt cattatgttg accctcgcct
      181 ctttcctgcc cgtcccgcct gccggagccg ccgtctttac ttggaaggac ggcggcggca
      241 acagctattc ggatgtaccg aaacagcttc atcccgacca aagccaaatc ttaaacctgc
      301 ggacgcgcca aaccaaaccg gcggtcaaac ccgcccaagc cgacgcaggg aagcgcacag
      361 acggcgcggc acaggaaaac aatcccgaca ctgccgagaa aaaccggcag cttgaggaag
      421 aaaagaaaag aattgccgaa accgaacggc agaacaaaga agaaaactgc cggatttcaa
      481 aaatgaacct gaaggcggtg ggaaattcaa atgcaaaaaa caaggatgat ttgattcgga
      541 aatacaataa cgccgtaaac aaatactgcc gttaatcggc tctagcgcaa acccgatgcc
      601 gtctgaagcg gcacggggtt tgtcatttct gccagtaggt tttgacgttg acgaactcgt
      661 acagcccgaa ttcggacaat tcgcgcccgt aaccggaatc tttgactccg ccgaaaggca
      721 ggcgcaaatc gctgctggta tggcggttga taaacaccga tcccgcctgt attttttcgg
      781 caaaccgcca agcgcgttcg gtatcggcgg tataaatgca ggcaccgagc ccgaacgggg
      841 aatcattggc aagggcgatg gcatgttctt cgttttcggc gcgcaaaatc agggcggccg
      901 gcccgaatac ttcttctctc cagacgcggc aggcaggatt taccctgtct aaaaccgtcg
      961 cgggataaaa ccagcctcgc ccttgtggga tttttccgcc ggtcaggcat accgcgccgt
     1021 ttgaaacggc atcttcaacc tgcccgtgaa ccctgtcccg caaatcttcg cggtgcagcg
     1081 gtgcaagcgt agtatcggga tgtttggggt cgcccatttt caatttagcg cattcggcaa
     1141 gaaacagcgt gataaaacga tcggctgcgg cttcggttac gatgatgcgc ttggcggcgt
     1201 tacacgattg ccccgcatcg cggaaacggg aataacaggc ttctgcggcg gcacgctcca
     1261 aatcagcatc gggcatcacg ataaaggcgt tgctaccgcc gagctccaac acggttttct
     1321 taaggtttgc gcccgcgtgt gccgcaagga tgcgccccgt atgcgttgaa ccggtaaacg
     1381 ccattgcatc ggtatcttca accgccttga gcgtgcccgc ctcatccagc cacacgcctg
     1441 ccagaggaat gccgtctgaa gccaaatcga acagtgcctg actgacgcgt gccacgctgg
     1501 gcgcgggttt gacggcgcac gcgttgcccg cgcacatagc gggaacggcg aaacgcaata
     1561 cctgccagac gggatagttc caaggcatga cggcaaacac cacgcccaaa ggctcgaagc
     1621 gcacctgact caaactcgcc tgcgtcgcga tggttttgtg ggcaagcagt tcgggggcaa
     1681 ggcgggcgta atagcgtatc agttcgatag acttgccgat ttccgcacgg cattcgtgca
     1741 agcagcgtcc gacttcctca cacaccattt ccgcaaaacg ctctttctcc gcctccaaac
     1801 ggtcggcaaa tttttgcagg cgcgcggcac gttcggttac gcccagttgc gcgaacgccc
     1861 cgccgcgcat tttcaaatcc gccagccgcc gttcaaactc cgcataatct tgagctggac
     1921 ggcggtaaag cgtttcgccc gtaaatacat tgacactgtg aaacatcgaa tcaacctgcc
     1981 agttgcggga atatcgtttt cagtcccgac acaataatct ccaccgatac cgccgccagc
     2041 atcataccca taatgcggtt taaaatcgtc agccccgtcg cgcccagcag gcggctgacc
     2101 ttcccggcaa cgattaaaat ggcataacaa atcgcactga ccaccaaacc ggccgcgata
     2161 atcaacgcga tgtcgccgta tgttttagcc gccgaagcgt aaataatcac ggtcgaaata
     2221 ccgcccgggc cgatggtgat cggtatggcg atgggcacga cggcaatcgc tccggcattg
     2281 cgggcggggc gcgcctgccc cgtttccggc tgcgcgccga gattctgctt ggcgggattg
     2341 tcgttgccgt tcatcataga aatggcaatc agcaacacca aaattccgcc gccgacctga
     2401 aacgaaccga cgctgatgcc caaaaccttc agcagcgtac cgccgatcag cgcaaatacc
     2461 gcaatcacgg caaacacggc aacggcggcc gtccgcgcga ccttcctgcg ctccttcgtg
     2521 ctgtgcccgt tggtcaggtc aaggtaaagc gacaacgcgc taaacggatt gatcagcacc
     2581 aaaaaagcca caatcagctt gccgatttcc atgcccaatc ccattatttc cccctccttc
     2641 aaacccgtgc ggcaggcatc cgatgctgca aattgccgcc gcaacggatt tttccgttat
     2701 aattaaaaat tcaagcaata cgccccatca tacccgaacg acggtatctt taccatcaga
     2761 caaggatgct tttcatggca ctgacacttg ccgacgtaga caaaatcgcc cgactctccc
     2821 gactgcacct gactgcggaa gaaaaagaaa aatcgcttca agaattaaac gacattttca
     2881 ctatggtcga acagatgcag aacatcaaca cagacggcat cgaaccgatg gcgcacccgc
     2941 acgaggccgc cctgcgcctg cgcgaagacg aagtaaccga aaccgaccgc gccgccgaat
     3001 atcaggcggt cgcaccggaa gtacgcgacc gcctgtacat cgtaccgcaa gttatcgaag
     3061 aataatccga atacgcttca gacggcatca gcaataccgc ccgaagccct ttaaggatgg
     3121 aagatttatg acccaataca cattgaaaca ggcaagcgtc ctgttgcagt ccaaacagat
     3181 ttccgccgtc gaactggcaa gcgcatacct tgccgccatc gccgaaaaaa atcccgccct
     3241 caacggctat atcaccatcg accaagataa aacccttgca gaagcccgtg ccgccgacga
     3301 acgtatcgcg cagggcaacg cctccgcgct taccggcgta cccgtcgcct acaaggatat
     3361 tttctgccaa accggctggc gcagcgcgtg cgcttccaaa atgctcgaca acttcatctc
     3421 cccctacacc gccaccgtcg tccaaaacct gctcgacgaa ggtatggtaa cgctcggccg
     3481 caccaatatg gatgagttcg ctatgggttc gaccaatgaa aactcattct acggtgcagc
     3541 caaaaaccca tggaatcttg agcacgtccc cggcggttcg tcaggcggtt ccgccgccgt
     3601 cgttgccgcg cgcctcgccc ctgccgcgct cggttcggac accggcggct ctatccgcca
     3661 acccgcatcg cactgcggca ttaccggcat caaacccaca tacggcacgg tttcccgctt
     3721 cggtatggtc gcctacgcct ccagcttcga tcaaaccggc ccgatggcgc aaactgccga
     3781 agactgcgcg attctgttaa acgcgatggc aggtttcgac cccaaagact ccaccagcct
     3841 cgagcgcgaa aaagaagact acacccgcga tttgaaccaa ccgctcaaag gtttgaaaat
     3901 cggcctgccc aaagaatatt tcggcgaagg caacagcgcc gatgttctga cggcattgca
     3961 aaacaccatt gatttgctga aagcccaagg cgcggaattg attgaagttt ccctgccgca
     4021 aaccaagctg tccatccccg cctactacgt cctcgcctcc gcagaagcca gcaccaacct
     4081 ttcacgttac gacggcgtac gttacggaca ccgtgccgcc caattcgccg atttggaaga
     4141 aatgtacggc aaaacccgcg ccgaaggttt cggcagcgaa gtcaaacgcc gcatcatgat
     4201 cggcacttat gtactgtcgc acggctacta cgatgcctac tatctcaaag cccaaaaact
     4261 gcgccgcctc gttgccgatg attttcagac ggcatttgca cggtgcgacc tcatcctcgc
     4321 gccgaccgca cccactgcag ccccaaaaat cggagcggat gcttcgccgg ttgaaaccta
     4381 cttgagcgat atctacacca tcgccgtcaa cctcgccgga ctgcccgcat tgaccctgcc
     4441 cgcaggcttc agcggcggcg gactgcccgt cggcgttcag cttgtcggca actacttcgc
     4501 cgaagccaaa atcctcggtg cggcgcatca aatccaactc aacagcgatt ggcacggcaa
     4561 acgacccgaa tgaagcagaa ccgcaccttt accttccccg attttcgcac cgtttacagc
     4621 tatgcgcctt tatatcggct gcaacattta aaatacacat tgcgaaaatt tttcggaaaa
     4681 aaagaaattt acgccttcga gcagtttgtc aacgcctccc ctatccgtca ggggctgttc
     4741 ctccactgcc cgcaaaatgc ctatccgctg ctgcgcgaat ttgttgacag gcgttttaac
     4801 tgcaaacgcc gtttagatgc gatgacggca gattttctca tggcggaaaa actgttcggc
     4861 acagacatcc tgcaccaaat ggaagactac cgcttccatt tggtcttggc gcacctttca
     4921 gacggcatca gcttgtggct caaccgcaac gacaactgcg tcgaagaagg cgcgtggtct
     4981 ttatctttgc gcgacgaagc aggcaaccgg ctgtatatgg cgactttcgc ctttgtcggc
     5041 acacacctgc tgacagcctc cgtacaaggg cctgcgggtg aagaagccaa agacactgtc
     5101 cgccgcataa ccaaacaact tcacggcttg cgtccccaac aactgatggt aaccgccctg
     5161 caatatttcg ccgccgtact cggcttggac ggcgcaatgg gcattgcaca aaaacatcag
     5221 gtcaaactgc gctggaaact taaaaagcgt gtcaaaatga attacgacgc attctggca
//
LOCUS       DAWHXZ010000149         5259 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000149 DAWHXZ010000000
VERSION     DAWHXZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.149"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            113..808
                     /gene="pgl"
                     /locus_tag="WIE35_001589"
     CDS             113..808
                     /gene="pgl"
                     /locus_tag="WIE35_001589"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ4154482.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            789..1775
                     /locus_tag="WIE35_001590"
     CDS             789..1775
                     /locus_tag="WIE35_001590"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ4154483.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1825..2673
                     /gene="hexR"
                     /locus_tag="WIE35_001591"
     CDS             1825..2673
                     /gene="hexR"
                     /locus_tag="WIE35_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ4154484.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2794..4440
                     /gene="pgi"
                     /locus_tag="WIE35_001592"
     CDS             2794..4440
                     /gene="pgi"
                     /locus_tag="WIE35_001592"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ4154485.1"
                     /translation="MKHFHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGSQKHDSSTERLINLYLQTNRK"
     gene            4454..5005
                     /locus_tag="WIE35_001593"
     CDS             4454..5005
                     /locus_tag="WIE35_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ4154486.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQLWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKVSELACKTVETEARLEELY
                     NRKKALIDEMVREADKKELPKRL"
BASE COUNT         1338 a         1464 c         1393 g         1064 t
ORIGIN      
        1 aaagcagtcc cgatatttgg tatcaaaagc ataaacaact gttcgaccgg catattgctc
       61 aacccgtttc cataacggaa tatgccgtct gaaataaaaa aaggacacaa atatgtttgt
      121 ttggcacgaa tacgaaaatg cggcagaagc ggcgcagtct ttggctgacg cagtggcgga
      181 tgctttgcag ggcgcactgg acgagaaggg cggtgcggtg ttggcagttt ccggcggacg
      241 ttcgccgatt gcatttttca acgccctgtc gcaaaaagat ttggattgga aaaacgtcgg
      301 catcaccttg gcagatgaac gcatcgtgcc gaccgtccac gccgacagca ataccggttt
      361 ggtgcgcgaa tacctgttga agaacaaagc ggaagcggca atgtggattc ctatggtgga
      421 agacggaaaa actgaaaccg aattacatcc cgatgctgtt gtcgattatg cactgaaaca
      481 ttacaaacag cccgatgttt tggttttggg tatgggaaac gacgggcata cggcttcgat
      541 tttcccgaaa gctccgcagt ttcagacggc aatcgacggt tcggcaggtg tcgcgctggt
      601 gcataccacg cccgttaccg cgccgcacga gcgcgtcagt atgaccttgg atgcgattgc
      661 ccatacgggg catgtgtttt tggcgatacg gggcgaagag aaaaaagccg tgttcgacca
      721 agccgcacaa ggcgaaaacc gcgaatatcc gatcaacctc gttttgaacc atcaaggagt
      781 gaactgccat gtcttctacg ccgaataaac aagccggata tccccgactg gtcgccgata
      841 tcggcgggac gaatgcacgc tttgcgctgg aaaccgcgcc gcgcgtcatt gaaaaagccg
      901 ccgtgcttcc gtgtaaagac tacgatacgg ttaccgatgc ggtgcgtgcc tatctgaatc
      961 aaagcggtgc aacagccgta cggcacgcgg catttgccat cgccaacccg attttgggcg
     1021 actgggtgca gatgaccaac caccattggg cgttttccat cgaaaccacc cgtcagactt
     1081 tggggctgga caccctcatc cttttgaacg actttaccgc gcaggcattg gcggtaacgc
     1141 agacttcaag caaagacctg atgcaggtag gcgggcaaaa gcctgtcgaa tttgccccca
     1201 aagccgttat cggccccggt accggcctgg gcgtgagcgg attggtgcac agccacgcag
     1261 gctgggtggc tttggcgggc gagggcgggc ataccagttt cccgccgttt gacgatatgg
     1321 aagtgctgat ttggcagtac gccaaaaaca aatacggcca tgtttccgcc gaacgctttt
     1381 tgagcggcgc gggcttgagc ttggtttacg aggctttggc tgcaaaacag aaagccaaac
     1441 ccgccaaact gatgccgtct gaaatcacgg aaaaggcttt gagcggcgcg tcgcctttgt
     1501 gccgtcagac tttggacatc ttctgcgcca tgctcggcac ggttgcttcc aacctcgccc
     1561 tgacgctggg cgcgcgcggc ggcgtgtacc tgtgtggcgg cattattccc cgcgtgttgg
     1621 aatatttcaa aacttccccg ttccgcagcc gtttcgagaa caagggcagg tttgaagcat
     1681 atcttgccgc gattcccgtg tatgtcgtct tgagcgagtt tcccggaatt tctggtgcgg
     1741 ctgcggctct cgacaaccat ttgagaaacg tttaaccaca gcggctcctt gcagcggggc
     1801 tgcattatcg aagggcatat cattatgtta agcaaaatca gcgaatcact ggcaaacctt
     1861 tccggtgcgg aacgcaaagt cgccgaatgt gcattggcgg aacccaaatg gttcgtccat
     1921 gccgccgttg ccgaaattgc cgaacgtgcg tccgtcagcc agccgaccgt catccgattc
     1981 tgccgcagct tgggttataa agggctgccc gagttcaagc tcgcgttgtc cgccagcatc
     2041 ggtcatgagg gtatgcccta tgtccatgaa gaactcaacg ccgacgacga tatggcaagc
     2101 gtggtcgaga aagtgttggg caatgccgcc gcctcgctgt tgggcgaacg ccgcttcctg
     2161 aaagagtcgg agctggaaaa cgccattgcc acgctgatgc acgcccgccg cgtcgagttt
     2221 tacggtgtcg gcaattccgg tatcgttgcg caggacgcgc aacacaagtt tttccgtttc
     2281 ggaatgtcca cggtcgccta tgtcgatacg cacacgcagc tgatggcagc atccgttttg
     2341 agcgatcagg atgttttggt tgccatttcc aacacgggtt cgtctatcga acttttggat
     2401 gcggtcagca tcgccaaaga aaacggcgcg tccgtcatcg cactgacccg caacgattcg
     2461 cctctggcgc aacttgccga ctgcgtgttg agcgttgcca cacaggaaaa tgccgaactc
     2521 tacacgccca tggtttcccg cctcttgcag cttgccgtca tcgacattct cgccatcgga
     2581 cttgccctgc gcttgggcga tgctgccagc ctgcaattgc agaaaagcaa aaaaagcata
     2641 cacaacaagc acatcgatta cgacaaagat tgaccttcag acggcatccc acaaatgccg
     2701 tctgaaatgc cgaacaacgg ccgtcggcgg cttgcggcag tttccagcag ccttttcatc
     2761 ccacaacaaa aaacctcatt caggagcata tagatgaaac actttcacga cttacccgca
     2821 tggtcgaaat tgtggaacca ctttgacgac agcaaaacat tgcatatgcg cgaaatgttc
     2881 gagcaagacc cgcagcgtgc ggaacgctac tggctgcaag tcggcggact gacgctggac
     2941 tactccaaaa accgcatcaa cgacgaaacc atgtcgcttt tgttcgagct tgcccgagaa
     3001 gcaggcgtgc cggagcggat gcggcagatg ttccacggcg aaaaaatcaa taccaccgaa
     3061 aaccgcgccg tcctgcatgt cgcccttcgc aaccgcacca attcgccgat tgtggttgac
     3121 ggtgaagatg tgatgcccaa agtcaaccgc gttttgcaac gtatgggcga atttgcacac
     3181 gaagtccgca gcggaagctg gctgggctat accaaccaag tcattaccga cgttgtcaac
     3241 atcggcatcg gcggatcgga tttgggcccg ctgatgatgt gtaccgcgct caaacctttc
     3301 ggtcatccgc gcctcaatat gcacttcgtc tccaacgtgg acggctccca actgcgcgac
     3361 gtattgtcca aagtccaccc cgaaaccacg ctgttcatca tcgcctccaa aacatttacc
     3421 acgcaggaaa cgctgaccaa cgcgctgacc gcgcgcgaat ggtttttgaa tcatgcgggc
     3481 gacgaagaag ccgttgccaa acacttcgcc gccgtttcca ccaatcaaaa agccgtcgcc
     3541 gaattcggca tcgacaccgc caatatgttt gaattttggg attgggtcgg cggtcggtac
     3601 agcctgtggt ccgccatcgg attgccgatt atgctgtatc tcggcgaaga aaacttcatt
     3661 gaaatgctca acggcgcgca cctgatggac caacacttca tcaacacacc gctcgagcgc
     3721 aacctgcccg tcattctcgc cctcatcggc atctggtata tcaactacta cggcggcggc
     3781 agccacgtca tcgcgcctta cgaccaacat ttgcaccgcc tgcccaaatt catccagcag
     3841 ctcgatatgg aaagtaacgg caaacaggtt acgttggacg gcaaagcagt cggacacgaa
     3901 acctcgccga ttatctgggg cgaaacgggc attaacggcc agcacgcctt cttccaactg
     3961 ctgcaccaag gcacgcacat tacccccatc gacctgattg cctcgcttga aaaacgcagc
     4021 aacctgcccg gacaccacga aatcctgctt gccaacgtct tcgcccaagc agaagccttt
     4081 atgcgcggca aaacccccga cgaagtccgc gccgaactca aagcgcaggg tatggatgag
     4141 gcgcgcatcg aagagctggt cccgcacaaa accttctccg gtaaccgccc gaccaacctc
     4201 atcctgatgg acaaggtcaa cccgcgtaat atgggcagcc tgattgccat gtacgaacac
     4261 aaaaccttcg tacaaggcat catttggggc atcaacagct tcgaccagtg gggcgtggaa
     4321 ctcggcaaac aactggctaa aaccattttg ggcgaactga ccggcgaaac cgggtcgcaa
     4381 aagcacgaca gctcgaccga acgcctgatc aacctctacc tgcagaccaa ccgcaaataa
     4441 aacctgcgga aaaatgccgt ctgaacgccg accgtttcaa acggcatttt tatcgaacag
     4501 gaaaaccgtc ggtaaacttg cagagcgtgt gcaatcccga tatgatggtt tgcataaatt
     4561 taaacatata tgttccgcag ctatggcact gattaaagag ccgttggaca aagcgaaaca
     4621 aaggaacgaa gaacttgaag cggcagaaga agcggcggcg caggaggcat tgggtcggga
     4681 gcaggaagtc gaccgcgtat ccgaatggga agaacgctac aagctgtcgc gcagcgagtt
     4741 cgagcagctc tggaaaggat tgcctcaaac cgtacagaat aagctgcaag cctcacagaa
     4801 aacatggaaa agcggtatgg acaaaatctg tgccaacaat gcgaaagccg aaggtgaaac
     4861 gccaaacggc ataaaagtca gcgaactggc atgcaaaacg gtggaaaccg aagcacgctt
     4921 ggaagagctg tacaaccgta aaaaagccct tatcgacgaa atggtcaggg aagcggacaa
     4981 gaaagaactg ccaaagcggc tctgaacagc gcggttcagg cactgcccgc cgatattgcc
     5041 gaaaccgtca tgcccgagta ccgcaactga caaaacggtt tgaatgccaa atgcgccgac
     5101 ggcgacgaat acggcgtggc acaatctgac tgccgtacca gagaaatcaa tgcgaaaacc
     5161 aaagaaatcc aaggttatct gattgactga aacttggatg cgggaatgtc ggcggctttt
     5221 gcgtttttgt cgtttttata gtgaattaaa tttaaaccg
//
LOCUS       DAWHXZ010000150         5173 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000150 DAWHXZ010000000
VERSION     DAWHXZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.150"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(73..861)
                     /locus_tag="WIE35_001594"
     CDS             complement(73..861)
                     /locus_tag="WIE35_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ4154487.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVGALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            1186..2541
                     /gene="yegQ"
                     /locus_tag="WIE35_001595"
     CDS             1186..2541
                     /gene="yegQ"
                     /locus_tag="WIE35_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ4154488.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRSIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSIAKQSQYVGHVTEIDAEGWATVEVKNR
                     FAIGDSLEIIHPSGNQTIKLEQMARKGQPVDVAPGNGIQVKIPNMRGKEKALIARVLN
                     P"
     gene            2675..3151
                     /locus_tag="WIE35_001596"
     CDS             2675..3151
                     /locus_tag="WIE35_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ4154489.1"
                     /translation="MLAACQSGSAPKNAHTASPGTPAGESCKVIRGDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGIGKSDSEAYERA
                     LINAARKFQQTAGKLGGRGVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
     gene            complement(3481..3678)
                     /locus_tag="WIE35_001597"
                     /pseudo
     CDS             complement(3481..3678)
                     /locus_tag="WIE35_001597"
                     /inference="COORDINATES: protein motif:HMM:NF013466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            complement(3682..4290)
                     /locus_tag="WIE35_001598"
     CDS             complement(3682..4290)
                     /locus_tag="WIE35_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ4154490.1"
                     /translation="MSNSSFKLSALAIACGLALSACSSGGGDAPSVKPTPTVPGASNP
                     APANPEPSAPAASPLKTGELGEKYFYGEGEITKLVINGETINLTVADEQLSRRVKLWT
                     DFDKIITSQGFENLIYGAAKLADGSYALFVQGKLSDSLPGDTVTYSGDVLHFRSRNLT
                     EGNWFNEGNSFRAAGSFTATVNFAGKSLNGKINSGDGLYMGT"
     gene            complement(4484..5113)
                     /locus_tag="WIE35_001599"
     CDS             complement(4484..5113)
                     /locus_tag="WIE35_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ4154491.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKKLPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
BASE COUNT         1365 a         1503 c         1151 g         1154 t
ORIGIN      
        1 caaaacgaag gctgtatgaa gggctgaaaa ccgaccctgc cgtctgaaac cggcagggtt
       61 gttattatgc gttcaaatca cgcccaaatc ttgcaagccc ctcaacacac cgtcctcatc
      121 aacgctgggg caaacatatt tcgccgcctc tttcgccgcc tgttccccgt tgcccattgc
      181 cacgccgaac ccgacttctg acagcatttc cacatcgttc aaaccgtcgc cgaacgccat
      241 cacgtctgcc atttccaaac ccaatgcccc aaccacgctt ctgatgccgt ctgttttcga
      301 cattcccgca ggcagcagat cgaccgcttc ctcgtgccag cgcaccgttt tcaagccttc
      361 ccgttccaca atatccgacc agggcggcat ttcgttttcc tccgcaaaca ccagcatctg
      421 atacaccggt ttgcttgaaa aataatcctt atcggcaaaa aaatcgctgg cgatatgctg
      481 caaggcgcgg cacacgcatt ccgacagcgc ggacacggcg atcccctctc cgccgacaaa
      541 cgcataatcc atgcccaagc catccaaatg cgcgcaaacc ctgcccatca aaccggcatc
      601 catcggtact tcgcgcacag ttttaccgtg cagcagcgca aactgtccgt ttatcgttac
      661 cacggcatcc atacccgttt ccgccatcat gtccctgacc ttttcgggaa tcgtcgccaa
      721 agaccgcccc gttgccaacg ccgtcaatat gcctttgccg cgcaaagccg ccaccgccgt
      781 tttcacggaa ggacgcaagg tatccgtata tttgcggtac agcgtatcgt caatatcgaa
      841 aaacacgatt ttaggattca tcacattctc tctcgcattc aaactgccgc attgtatccc
      901 aatcaggcaa atacttgata aatccttata aatttcccgg caaaattgac cgcgaacacg
      961 aaaaggcgga taatccgccc atcctcaaac ccttttcaga cggcatttgc agcaatgccg
     1021 tctgaaacat ttttacaaag cacacaaatc atgtttcaac acacaggacg acacataaag
     1081 cgtcgcccta tatgttgccc tgattcggaa agggttacac ccctcccaaa taaagtctga
     1141 ttctaccgcc ctaaagggcg gggtttcaac cgaaaaggaa acacgatgaa agcacccgaa
     1201 ctcttattgc ccgccggcgg attggaaaga atgcgcgccg cctacgatta cggcgcagat
     1261 gccgtttacg ccggcagccc gcgttactca ctgcgcgccc gcaacaacga atttgccaaa
     1321 ctcgacgttc tcgaacaagg cattaaagaa gcgcacgagc gcaacaaaaa attcttcctt
     1381 accgtcaata cgctgccgca caactccaaa ctcaaaacct tcgtctccga tatggagccc
     1441 ttgattgcga tgaagcccga cgcgctgatt atggcggatc cgggtttgat tatgaccgtg
     1501 cgcgaaaaat ggccggaaat gccgatccac ctgtccgtac aagccaacac caccaactac
     1561 tggggcgtga aattctggca gaatatcggc gtcgaacgca tcatcctgtc gcgcgaattg
     1621 agtatggaag aaatcgccga aatccgccaa caatgccccg acatcgaact cgaagtcttc
     1681 attcacggcg cattgtgcat cgcctactcc ggccgttgcc tactctccgg ctacttcaac
     1741 caccgcgacc ccaaccaagg cacctgcacc aactcctgcc gttgggacta caaagtccac
     1801 aacgctacgg aaagcgatgc gggcgatgcc cagcttctgc aaggtttcaa ctttgaaaaa
     1861 gcccaagaag aagccaacca aaactttgaa ggcatcaacg gtcaaaaacg ccatccctac
     1921 gccgacaaag ttttcctgat tgaagagtcc aaccgcccgg gcgagatgat gccgattatg
     1981 gaagacgaac acggcaccta catcatgaac tccaaagacc ttcgcagtat tgaagtggtt
     2041 gagaaactcg ccaaaatcgg cgtggacagc ctcaaagtcg aaggccgtac caagtcgctc
     2101 tactacgttg cacgcgtggc tcaatcctac cgcaaagcga ttgacgatgc cgttgcaggc
     2161 cgtccgtttg attacagcct gttgagcgaa ctcgaaggcc tcgccaaccg cggctacacc
     2221 agcggcttcc tcgaacgcca ccaaacccaa gactatcaaa actacctgac cggccactcc
     2281 atcgccaaac aaagccaata tgtcggccac gttaccgaaa tcgacgctga aggttgggca
     2341 acggtggaag ttaaaaaccg ctttgccatc ggcgattcgc tcgaaatcat ccacccgagc
     2401 ggcaaccaaa ccatcaaatt ggaacaaatg gcccgaaaag gccagcctgt cgatgttgca
     2461 cccggcaacg gcatccaggt caaaatcccc aatatgcggg gcaaagaaaa agccctcatc
     2521 gcacgcgtat tgaacccctg agccacaatg ccgtccgaac cccgttttca gacggcattt
     2581 tgttgaatct tacggtacaa tggcaatgat ttcccaccgc aagcaaggat atccgacatg
     2641 aaaaaaaatc ctcatactct ccgccgccgc cctgatgctg gcagcctgcc aatccggcag
     2701 cgcacctaaa aacgcgcaca cggcatcccc cggcacaccg gccggagaat catgcaaagt
     2761 catccggggc gacaccgcag gaagcggcaa aaacatcatt taccgttgct gcaacggtca
     2821 ggcggcaata aatgcagcca ttgcagacgg cgtattggaa tcagggattc ccgtcagctt
     2881 cggcggcggg ggcgtaccgg ttgcaaacgg caggaatcta gttacccgtc aggcggcaaa
     2941 cggcataggc aaatccgact cagaagccta cgaacgcgcc ctaatcaatg ccgcccgcaa
     3001 attccaacag accgcaggca aactcggcgg acgcggcgtt accggcttcc acagctactt
     3061 tgacaagcaa ccgctgcaag gcgggcaata cgactgtcag gcaggctcgt tccacgtccg
     3121 cgtcgtcatg cgcgccaatg tcgtccgtta acatatcggc aatcaaaaaa tgccgtctga
     3181 aacccgtttt cagacggcat tttttcaggc tttaccggca attccttcaa catcccactc
     3241 cggctgtgac tgtaacactg tagcagcaag gtatacggat atatagtgga ttaacaaaaa
     3301 tcaggacaag gcggcgagcc gcagacagta caaatagtac agcaagacga gccaacgctg
     3361 tactggttta aatttaattc actatattat cactttccgt catcctaacc atataatatc
     3421 aaacaaaagc cccggtctat ccgcaccggg gctttcgtca aatcaaggat tcaaacggga
     3481 ttattgggtt ttcttaccac caaatacgcc gaagctgtta tttgtatatg tcttggcctg
     3541 ttttggttca tccttaacat agctgtacct gcctgccatt tctgcagcct tggcaccata
     3601 gaatcctcca gagatactac ctttctcacc gtttttccaa gtaccggtaa agcggttgcc
     3661 atcaagactt ccctcgaatt cctaggtacc catatacaat ccgtcaccac tgttaatctt
     3721 accgttcagg gatttaccgg caaaattgac agtagcagta aacgaaccgg ctgcacggaa
     3781 tgaattacct tcattaaacc aattaccttc ggtcaagttg cgcgaacgga aatgcaatac
     3841 atcgccgcta taagtaaccg tatcgccggg cagactgtcg ctcaactttc cttgcacaaa
     3901 gagtgcgtat gaaccatctg ccaatttagc cgcaccataa ataagatttt caaaaccttg
     3961 ggaagtaata atcttatcga agtctgtcca aagcttcacc ctccttgaca gctgttcatc
     4021 agccactgtc aaattaatag tttcgccatt gatgaccagc ttcgtgattt caccttcccc
     4081 gtaaaaatat ttttcaccca actcaccggt tttcaatggt gaggcagccg gtgctgaagg
     4141 ttcgggattg gcaggcgccg ggtttgatgc acccggaaca gtcggggtag gcttaacact
     4201 tggcgcgtct ccgcctcccg aactacaagc tgaaagagct agaccgcagg cgatggcaag
     4261 tgcactcaat ttaaatgacg aattcgacat aacattctcc taatgacatg gataacaaaa
     4321 ataagttcat cctcttttac gaaaaggcat gatgcttatt cataaattta ttgaaaaaaa
     4381 atttcaagtt ttcatagtcg gttgcaaata tgttccggca aacagctgcg tatcatataa
     4441 agcgcaactc cataaaaaca ccgatacctg atttttgcat tactcaatgc ctgtgcggca
     4501 tcattccttc cggcgcttcg gcatcagcgg ccaagccaaa ggtttcgcgc aacacgactt
     4561 tgtaaaatgc aaaggcttcg cgcgcgcctt ggatggcttc cgcttcggct tcgggagtca
     4621 ggttcaaagc gttcagatgc tcgacgaagg cgcgccagtg tttgccgcga ccgtcgggat
     4681 ggggagcgag gtggcgcgcg ccgtgttcgc cgttgtaatc gagtttttgg gcgtgtttga
     4741 acaaaaacgc cgcgcccaaa ttggaacctt cggcgcaata aagccagccg attgctttgt
     4801 tgccggtttc gtgtggcaat tttttgtcga atttgtaagg ctcttcgccc aagtctttca
     4861 agtcttgcgt tacggcatcg tatcgcgcca tatattccag ctcgggaatg gctttgttta
     4921 attcggcatc tttatagatg tggtcgacgg ccttgtggaa aacggattgg agtttcaaaa
     4981 atttaatgta gttttctttg ctgacaaacg gttggacaga cataacgagg ttatccacgc
     5041 tgtcgtgaac cgccgtggta tccgccttca gacgtttggc aaacgtcagg gcttgatttt
     5101 cggtttcact cataagtttt cctttgtcgg taagggataa ggatgcgggg cgggcaaaat
     5161 ccgcacttgc ccg
//
LOCUS       DAWHXZ010000151         5130 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000151 DAWHXZ010000000
VERSION     DAWHXZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.151"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..543)
                     /locus_tag="WIE35_001600"
     CDS             complement(<1..543)
                     /locus_tag="WIE35_001600"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ4154492.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIR"
     gene            671..1288
                     /gene="gmk"
                     /locus_tag="WIE35_001601"
     CDS             671..1288
                     /gene="gmk"
                     /locus_tag="WIE35_001601"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ4154493.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            1347..1553
                     /locus_tag="WIE35_001602"
     CDS             1347..1553
                     /locus_tag="WIE35_001602"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ4154494.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            1646..3802
                     /locus_tag="WIE35_001603"
     CDS             1646..3802
                     /locus_tag="WIE35_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ4154495.1"
                     /translation="MPVPQPTAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGENLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGKHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3869..5053)
                     /gene="coaBC"
                     /locus_tag="WIE35_001604"
     CDS             complement(3869..5053)
                     /gene="coaBC"
                     /locus_tag="WIE35_001604"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ4154496.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNKSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAGYL
                     NK"
BASE COUNT         1327 a         1284 c         1286 g         1233 t
ORIGIN      
        1 acggatattc ttgccgcctt gaaggtcggt aaattccaaa atggcggcgg cttcgacaat
       61 ttccccgccg agtttgcgga tcagctccag tccggcaagc atcgtgccgc ccgtggcaac
      121 caaatcatcg accagcagca cgcgcgaacc gggtttgacg gcatcggtgt ggatttccac
      181 cgcagcttcc ccgtattcga gcgcgtagct ttgcgatacg gtttcaaaag gcagcttgcc
      241 ttttttgcgg atggggacga aaccgacgtt gagctggtag gcgagtgccg cgccgataat
      301 gaagccgcgc gcgtccaaac cggcaacgat gtcgattttc tgatccatat agcggtaaac
      361 caataaatca accaaaagtc ggaagtattc cgcgctttgc aggacgggcg tgatgtcgtg
      421 gaataagatg cctttttgcg gccagttttc gattttgcgg attttgtcgg caagcgcgcc
      481 gacactcata gcttcgggat gaaccagcat tgcgtgttcc aagtttgatg tttttaacgc
      541 catattgtag ccgtcagcct gccgcttgcc aaatcaatca aaacaatagc ggcaaaatat
      601 gggcatcggc aaataggaaa ccgagtgtcc gttgtgatac aatcggctgg aaaatataca
      661 ggtaaaaaat atgtccgctt atcgaaaagg caacatcttt atcatttcgg ccgcttccgg
      721 cacgggcaaa accacgctgg tgtcgcggct gttggcaaac cataacggtt tgcgcgtttc
      781 cgtatcgcac acgacgcgtc cgccgcgtga aggcgaagta aacggcgtac attatcattt
      841 tgtttctaaa gaagagtttg agtcgcttat cgcgcaggaa gcttttttgg aatacgcaga
      901 cgtgttcggc aactattacg gcacaagcgc ggagggcgtg aatgctttgg cggcggcagg
      961 ctatgacgta attttggaaa tcgacgttca gggcgcggcg caggttcgca acgcgctgcc
     1021 ggaagccgtc ggcatcttta tcctgccgcc gtctttcgat gtgcttgctg cacgtctgaa
     1081 cggacgcggg acggacagtc gggaagttat ccaaaggagg ctgtcgaagg caaggcatga
     1141 aatcgaacag tccgtattgt tcgattttgt cgtggtcaat gatgacttgg cgcgggcgga
     1201 ggaggatttg cgccatattg tgaatgcctg ccgtctgaaa aggtcgcggc aactgggatt
     1261 tattgcagat ttgttggaaa attcctaaaa aaccgcgaaa atacccagtt tcctatttta
     1321 aatatttttg aaagaaagca aataatatgg cacgtattac caccgaagac tgtaccggaa
     1381 aaatttccaa ccattttgac ctgacattgg tagcggcccg tcgggcccgc cagcttgaaa
     1441 atggcaatac gccgcttgtg gacgatgtcc gcaataacaa accgaccgtt accgccttaa
     1501 gggaaatcgc cgccgggcat atcggtacgg aactgttgac gcgcaataaa taaattctgc
     1561 cggaaacgca cgtcggcaca ttctgccgcc gtgcagtccg acggtttgaa acgaaaaata
     1621 aaatatgttc cgaaagccgt ctgaaatgcc tgtccctcaa cccaccgcac cttacgaccc
     1681 cctgaccgcc gaagcgcgtg ccctgctttt ccataccgcc tcctacctca agcccgagga
     1741 acaggcggag cttgaaaaag ctgtcgccta tgcgtttcgc gcccacgacg ggcaaacccg
     1801 caaaagcggg gagccctaca tcacgcatcc gattgccgtt gcgacgcagc tcgccctttg
     1861 gcatatggac atacagggtc tttgtgcagg cgtgatgcac gacgtattgg aagatacggg
     1921 cgtgacaaaa ggggaaatgg cggcggtgtt cggcaatacg gttgccgaga tggtggacgg
     1981 gctgtccaaa ctcgaaaaac tcaaatttga agatcatgca gagcatcagg cggagagttt
     2041 ccgcaaactg attttggcaa tgaccaaaga tgtacgcgtc atcgtcgtca aacttgccga
     2101 ccgtctgcat aatatgcgga cactcggttc gatgcgcccg gacaaacgcc gccggattgc
     2161 aagggaaacc cttgaaatct atgcacagat tgccaaccgc ataggtttga ataacgcata
     2221 tcaagagctt caggatttat cgttccaaaa cctgcatccc aaccgctacg agactttaaa
     2281 aaaagcgatg gacaagagcc ggaagaaccg gcaggacgtt gtcggcaaag tcttgcgcgc
     2341 attcggccag cggctggtag gcgcgaatat agaggccaaa atcaaaggca gggaaaaaaa
     2401 cctgtacggc atccatcaga aaatgatggc gaaaaagctg cgctttgccg aggttatgga
     2461 tatttacggt ttccgcgtca ttgtcaacag cattccagcc tgttatgccg cactcggcgc
     2521 attgcacacc ctctatcagc ccaagcccgg gcggttcaaa gactatatcg ccattccgaa
     2581 aagcaacggg tatcaaagtc tgcatacgac tttggtcggt ccttacggct tgccgattga
     2641 agttcagata cgtaccaagg aaatggatgc tgttgccgaa ggtggaatcg ccggacattg
     2701 gagctataaa tcatattcta agacggtcga tcaggcggtg cttcacacaa accggtggct
     2761 gaaaaatatc ttagatttgc aggcaagcag tgccaatgcc attgagtttc tcgaacacgt
     2821 caaagtcgat ttgtttccga acgaaatcta catccttacg ccaaaaggaa aaatcctaac
     2881 tttgcccaaa ggggcaacgc ctgtcgattt tgcttatgcg gtgcataccg atatcgggca
     2941 caaaaccgtt gccgcacgta tcaacaatat catgatgccg ttgcgtacga agctcaaaac
     3001 cggtgattcc gtcgaaatta ttacatccga acacgccaaa cccaatcccg cgtggttgaa
     3061 tttcgccgtg tcaggcaggg cgcgcagtgc catacgccaa tatattaaaa accttaaccg
     3121 gcacgatgcg gtcgttttgg gagagaacct cttacaaaaa gccctgtcca gtttgctgcc
     3181 caaagatgtc ctgctttcag acggcatcaa agaaaaatac cttgccgatc tcaacgataa
     3241 acagacttca tttgaggaag tgctgtacaa cgtcggtatg gggcataccc tgcctgttta
     3301 tgtcgctatg catattgccg aattggcagg aaagcatttc ggtagtgagg tcaggctcag
     3361 ttccattaaa gtcgatgggc aggaaagcgg gcatattcat tttgcagagt gctgccaccc
     3421 tgttcccggc gattccatcc gtttgctgtt ggttaaggga aaaggcatga ttatccacag
     3481 agatacctgc ccgacgttgt tgaagtccga tcccgaacag cagctggatg cagactggga
     3541 aaatatgaac gggcagaact accgtgtcgg gcttcaagtc caatcggaag acagccacgg
     3601 cctgttggca ttaatggcgc aagcgatttc cgattccggt gcggacattg agtcggtcga
     3661 aacaccgtct aaatcccagt cgggaacgga aggttttgtc gaattcaaat tcttattgaa
     3721 agtcaagaat ctgaatcaat tgaatcagat tattcaaaat ctgcatagta ttccatatat
     3781 ccgcaaagtc atcagaagtt gaagcaggta aaacgggttg ccttttgatt aacaggcaac
     3841 ccgctttatg gtttatccgt tcaattgctt atttgttcaa atatccggca agcctttcaa
     3901 caatctgcat cgccgcttca tctttacttg tttcgggaaa agatagttcc ccgtcgtccc
     3961 cgataatggt aatccggttg gtcggtttgc ccatcgcgat tgaaacatca ttggcaacta
     4021 tcaccggtat ttttttctta atacgttttt ctcgtgcata tgccattaca ttctccgttt
     4081 cagcggcaaa accgatgcag aacggcgggt tcggcaatga ggcaatagaa gccaaaatat
     4141 cggggttctc atccaattcg atggataacg gtttggcatt tttatctttt ttgaattttt
     4201 gcgtactctt gtttttaacc ttatagtctg agacggcggc aacagaaata aaagcatctt
     4261 gtttttcaat caaacgatgc actgcgcgat gcatatcttc agcgctgacg gcttgaaccg
     4321 tatcggatat gccgaaaggc agcgcggttt gaagctgtcc gtgaatcagg ctgacttccg
     4381 caccggcggc acggcacgcc cgcgccaaag ctacccccat ttttccgctg gaggtatttg
     4441 tgatgcccct gacagggtca atggcttcaa atgtcgcacc tgcggtaatc aagattttct
     4501 tgccccttaa aattttcggt gtccacaagt ccggaagcaa atccagcaat tcggcaggtt
     4561 ccggcatcct tcccatgcca ttttctccgc aagcctgttc gcccaagccc ggcatataga
     4621 cagtaatgcc gtctgaaacc agttgtgcga tattccgttg gttggcaggg ttgagccaca
     4681 tttccacatt catcgcggga gcgatggcaa gcggacattt ccgtgcggct gccagactgg
     4741 tcagtaggtt atctgccacg ccgttacaga tttttgccac ggtattcata cttgccggcg
     4801 caatcagaaa aacatccgca ttccgggtca ggttgatatg ttccataccg tttgaaccgt
     4861 tgccgccgtg cgtgtcggtc aggacaggat tgccgcttaa agcctgaaaa gtcagcggag
     4921 aaacaaattc agttgccgag cggctcataa ccaccgtaac cgaatgcccc tgttttttca
     4981 gcagacgaac caactcgcaa gacttatacg ccgcaatact gcccgttaca cctaaaagaa
     5041 tatgtttgcc cattttgaca atttctaatt acgaagaaaa aacagcagcc attctataac
     5101 aaagtgtgga tacattagat accgtttgga
//
LOCUS       DAWHXZ010000152         5116 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000152 DAWHXZ010000000
VERSION     DAWHXZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.152"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..554)
                     /gene="pepN"
                     /locus_tag="WIE35_001605"
     CDS             complement(<1..554)
                     /gene="pepN"
                     /locus_tag="WIE35_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ4154497.1"
                     /translation="MSKIVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASAGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSDGRHWVKWEDPFAKPSYLFA"
     gene            complement(690..1484)
                     /locus_tag="WIE35_001606"
     CDS             complement(690..1484)
                     /locus_tag="WIE35_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ4154498.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLHGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(2234..3103)
                     /locus_tag="WIE35_001607"
     CDS             complement(2234..3103)
                     /locus_tag="WIE35_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ4154499.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWDGKKRETVLKQHFKHMAKLMLEYGLYWYAPAKRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLQFYPAWKSFPGEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSSDFY"
     gene            complement(3154..3690)
                     /gene="ruvC"
                     /locus_tag="WIE35_001608"
     CDS             complement(3154..3690)
                     /gene="ruvC"
                     /locus_tag="WIE35_001608"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ4154500.1"
                     /translation="MAASVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(3693..3932)
                     /locus_tag="WIE35_001609"
     CDS             complement(3693..3932)
                     /locus_tag="WIE35_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ4154501.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMGQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(3963..4973)
                     /gene="dusB"
                     /locus_tag="WIE35_001610"
     CDS             complement(3963..4973)
                     /gene="dusB"
                     /locus_tag="WIE35_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ4154502.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTKKTLHRSDFTDEGGIVAVQIAGSDPQQMADAARYNVSLGAQVIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHKNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAQTVLKQTAV
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDSAGV
                     RIARKHIGWYIGEMPDGEQTRRDINRLDSAAAQYDTLAGYLERVAEKTDGWACAYRQN
                     AV"
BASE COUNT         1222 a         1355 c         1249 g         1290 t
ORIGIN      
        1 aaagcaaaca gataactcgg tttggcaaac gggtcttccc atttcaccca atggcggccg
       61 tctgaatact cgccgccgtc gattttgttg ccgttggaga gcaaaacggg atagcgtttt
      121 ttgtccgcga cgatggtggt cgtgaacttg gacatgacat ccggacggtc gatatagaac
      181 gtgattttgc ggaagccctc cggctcgcac tgggtaaaca ggttaccggc ggacgcatac
      241 agccccatca gcgatttgtt ttccgccggc aggatttcgg tttccacttc gacggtgaag
      301 cgttcggacg gcacgtctgc aatcgtcagc gtctcacctt ccaacacata atccgccgcc
      361 gccccgttga ttttgacgga caagagtttc gccgaaccgt ccaacaccag cggctcccct
      421 accctctgcg gctcaaccgt caaacgcgac ttcacgacgg tttgcggttc ggcaatatca
      481 aaatgcaggt cggtttcaag gatgcggtag gcaggcgttt ggtaatcttt cagataacgg
      541 acgattttgc tcattttttt ctttcaatat tgtttggttt gactggaaaa ggcttcagac
      601 ggcataggca aaccctgcac cgatatgccg tatgaaaccg aaacatcagt gcggacgcac
      661 cgtcaaacaa ccggttttaa tttcaatctt tatttccacg cttggacaaa ttctgcccaa
      721 tgcggtttgt cttctgtttg ggcggaaaga tagtttctga tccgcttgat ttccatttcg
      781 tattcgtccg cgtcgaatct gccgctgacc agacagaaac gcagatacat caaatacgta
      841 ttcacggcat cggtttcaca ataattgcgg atttccttca gcctgcccgc gtggaacgca
      901 tcccacacct tgctgccgtc catacccagc ttgcctggaa aaccgcacag tttcgccata
      961 tcgtccagcg gcacgtttgc cctcggctgg taaagcgcga gcaaatccat caaatcgcaa
     1021 tgacgttggt ggtagcggct gatgtagttg ttccacttga aatcgcggct gtcgccgaaa
     1081 tcgccgtcac ccatatccca atagcgcgcg gcgttgatgc cgtgtagcag cgaacggtaa
     1141 tgcagaaccg gcaggtcgaa accgccgccg ttccaactga ccagttgcgg cgtatgtttt
     1201 tcaatcaact cgaaaaattt ggcaatcacg acttcttcgc catcgtccat ctcgccgatt
     1261 gtaccgacat ggactttatc ctgcccccaa cgcatgcagc acgaaatcgc cacaatctga
     1321 tgaagatgat gctgcataaa atccccaccc gtctgagcac ggcgtttttg ctgggcaaac
     1381 agcaccactt catcgtcggg cagcgaggac ggcagctcgt acaatgttcg gataccctgc
     1441 acatcgggta cggtttcaat atcgaaagcc aaaatcgtgg tcatgacagc accttgtatt
     1501 taaaacagac ttgcgcctat tgtgtcatta aaaggcggat aaaaaaagag gcaacccccc
     1561 acaggattgc cccaatacct caaatcagag atttacgctt cacaaacaat acaggctttc
     1621 gcctgcggct ttgcccgcgt agctcaactc tacgccggca aacttttgtt tcaccgtttc
     1681 cgatgaaacc ccgaccaatc gcaagactga ccggaaaatc ctttcagacg gcatttcctg
     1741 cctgtcgtgt aattccatgt agcgaaatgt acgccatttt ctacgctttg ccaagcattt
     1801 tttacaatat aaatgtcaaa acattaattt tataaaattg ctgaaaatat taaatatatg
     1861 gatttttatt ttttatattt caataaatat aaatttaatt ttgatttata tttaaattta
     1921 agcataaaat gtcaaatatt aaaggaaaca tgaaaggcat atattaaata tttatttata
     1981 acgctatgtt tttaaagaaa attaatttta atatattaac tagattgtct gcatatattc
     2041 ataggtttgc ggtatttctt ccaaaacctg cttcgaattt cccgaccaag tcttaaaaat
     2101 attgtttttt gagatactta aatagcagcg attatcaaat gaaatctgtt catataatct
     2161 gccattttgc atttaaaaaa acaatcagga gtttcgactc gaaacgcctg atatgttttg
     2221 taattttacg tagtcagtaa aaatcggagc tgccttccgg acgggtttta aaacgcttgt
     2281 gcagccaaaa atactgctcg ggatgttcgc gcacgcgttc ctcaataaag cggttcatac
     2341 gttgcgcgtc ggcttgcgcg tcttcacccg gaaaggattt ccaagccggg tagaattgca
     2401 gtgtaaccgt attgtccgcc tccctgacgg ggatggcggg aatcactttg gcatttgcaa
     2461 gcgcggcaat gcggctcaag ccggtaatcg ttgccgtctg aataccgaaa aaatccacaa
     2521 aaaccgaatc gttgcgtccg aaatcctgat cgggcagata cagaaacggc gcgctgcttt
     2581 tgcggaactg tttgacgagg gcgcgcagcc cttcggtgcg cccgataagg aaaacgttgt
     2641 gataacggtt gcggcctttc aaaatctgtt cgtccaatat cttgtttttt tggtgggaat
     2701 acatactaat tagcggaaca tcctgattga gcgcgtacac cgccatctcg aacgcggtaa
     2761 agtgcggata caggatgatg actttttccc ccgccgccag tgcgtcgtcc aaataatgct
     2821 tattgcggta acgcaccagc gatttcaggc gtttagcagg cgcataccaa tataagccgt
     2881 attcaagcat cagtttcgcc atatgtttga aatgctgctt caatacggtt tcgcgctttt
     2941 ttccgtccca ctcgggaaag cattttgcca aattgatttc gccgatacgg cggcggggtt
     3001 tgaccaaaag gtaggcgagc aagcctgtca gatcggcaag tttgtgcagc agcgcaaacg
     3061 gcagaaactg caaaacatac agtacaaaaa atataaattt catctcgata cacattttct
     3121 tttcaggcgg caaaatacaa atgccgtctg aaactattga aacctgccgc gcttgacctg
     3181 catccccgaa ggattgagtt tggcggcaag cccgtggttg cgtaaggcgt gggtcagcgc
     3241 gacggcaaga ccatccgccg catccgcctg cggcgttccc gataagccga gcatctgcac
     3301 caccatatgc tgcacctgtt cttttgccgc cttgcctttg ccaactaccg cctgtttgac
     3361 ctgcaaggcc gtgtattccg aaacgggcag cttatggctg accaatgccg ccaatgccgc
     3421 gcccctagcc tgccccagca tcagcgtcga tgccggattg acgttgacga acacctgttc
     3481 caccgccgcc tgttgcggct tgtaaacggc aacgacttcg ccgatatgcc gcacaatcac
     3541 ggcaatcctg tctgccagag gcgcatcggc aggcgttttg atgcagccgg aggcgacgta
     3601 aaaatgatcg cgccccctga catcgatgat gccgaaaccc gttacacggc tgcccgggtc
     3661 gatgcccaag atacggacgc ttgcagccat attcacaaca aaccgtgttg aatcagcttc
     3721 ttacgcaggg tattgcggtt cagccccagc atcacggatg ctttggactg gttgccgccg
     3781 cattgcccca tcacgcacac cagcagcggt ttttccacct gatgcaagac catatcgtac
     3841 acgccgcaag gttcggtacc gttcaggtct ttgaaatatt gttccaaatt ttgtctgata
     3901 cattgggaaa tatcgggaag ggtatggggc ataatcgcgc tttcaaaagg ataatcgggt
     3961 gttcagacgg cattttggcg gtaggcgcac gcccaaccgt cggttttttc ggcaactctt
     4021 tcaagataac cggcaagtgt gtcgtattgc gccgccgcac tgtccaatcg gttgatgtcg
     4081 cggcgcgtct gctcgccgtc gggcatttcg ccgatgtacc agcctatgtg tttgcgtgcg
     4141 atgcgtacgc ctgcactatc gccgtaaaac gcgtgtatgg cgcggatgtg gttcaaaatg
     4201 gcggcggcgc attctgccaa actcaaggca ggcggcaaaa caccgtgttc ggcataatgt
     4261 ttcaaatcgc ggaagaacca cggcctgcct tgcgcgccgc gccctatcat aatgccgtcg
     4321 acggcggttt gtttgaggac ggtttgggct ttttgcggcg aagtaatgtc gccgttgacc
     4381 cagaccggga tgttcagacg gcatttggtt tcggcgatga gttcgtaacg cgcttcgcct
     4441 ttgtacattt gcgtacgcgt gcgtccgtgg acggcaaggg cggcgatgcc gcaatcttcg
     4501 gcgattttgg cgataacggg caggtttttg tggtcgtcgt gccagcccaa acgggttttg
     4561 agggtaacgg gtacgcccgc cgctttgacc accgcctcca aaatggcggc aaccagcggc
     4621 tcgttctgca tcagcgcgct gccggcttgg acgttgcaca ctttcttggc gggacaaccc
     4681 atattgatgt cgatgacctg cgccccgagg ctgacgttgt aacgcgcggc atcagccatc
     4741 tgctgcggat cgctgccggc aatctgcacg gcaacgatgc cgccttcatc ggtaaaatcg
     4801 ctgcggtgca gggtcttttt ggtatttctc aacgtcggat cgctggtcag catttcgcac
     4861 accgcccaac ctgcgccaaa atctcggcaa aggcggcgga acggcttgtc ggtaatgcct
     4921 gccatcggcg caagtgcgat ggggttgtcg ataaaatagc cgccgatgtg cataatgggt
     4981 ccgcgttcca aaaaagtgcg acattgtaca ttttttaagc aaaatttcca atctctgcct
     5041 gcacccgcga ttggggcaga caccgtttta tggcataatc cgcacacaga ttccctgccc
     5101 cgccactcac aggaag
//
LOCUS       DAWHXZ010000153         5037 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000153 DAWHXZ010000000
VERSION     DAWHXZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.153"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            46..237
                     /locus_tag="WIE35_001611"
     CDS             46..237
                     /locus_tag="WIE35_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154503.1"
                     /translation="MIQTILEDGDIEVKNLKTENYSGKAREILGDSYPIEHISKNIST
                     IQNIISEFMIQKQTPAPII"
     gene            257..460
                     /locus_tag="WIE35_001612"
     CDS             257..460
                     /locus_tag="WIE35_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154504.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            487..1449
                     /locus_tag="WIE35_001613"
     CDS             487..1449
                     /locus_tag="WIE35_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ4154505.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDATGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGTVVLSASDKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            1496..1867
                     /locus_tag="WIE35_001614"
     CDS             1496..1867
                     /locus_tag="WIE35_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ4154506.1"
                     /translation="MKRQAFFKPIACAAFLSAISLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLILIDYFVPPELFVKLGQHL"
     gene            1893..2819
                     /gene="fabD"
                     /locus_tag="WIE35_001615"
     CDS             1893..2819
                     /gene="fabD"
                     /locus_tag="WIE35_001615"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ4154507.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFTGQAVVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAESTQGEVVEAVNFN
                     SPGQVVIAGNTAAVERAMLAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIEAH"
     gene            2958..4823
                     /gene="msbA"
                     /locus_tag="WIE35_001616"
     CDS             2958..4823
                     /gene="msbA"
                     /locus_tag="WIE35_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ4154508.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
BASE COUNT         1228 a         1583 c         1180 g         1046 t
ORIGIN      
        1 gataccgaat taactatcta ttttgaaaaa ggatattatt tgccaatgat tcaaacaatt
       61 ttagaagacg gggatattga agtcaaaaac cttaaaacag aaaattacag cgggaaggca
      121 agggaaatct tgggagattc ctaccctatt gagcatatct ctaaaaatat ttctacaatt
      181 caaaatataa tttctgaatt tatgatacaa aaacaaacac cggcaccaat aatttgatgg
      241 aaaaccaaca atcaaaatgc cgtctgaagc atgtttggtt ttcagacggc atttccttcg
      301 cttaaaacag cgtatcggca accccgccct gcctgtccac ggcaatctgc atctgaaaac
      361 catctgtatc ccaaaccaca cccccatccc tgtttccgtc atttgcaccc tgcccgtatt
      421 gggcaatcat cggtttttcg cttacaatag ccgaatctga accaactctc taaaaaggcc
      481 gttcccatgc agtatgcaaa aatttccggc acgggcagct atcttcccgc caaccgcgtc
      541 agcaatgacg accttgccca aaaggtagat acctctgacg agtggattac cgcgcgtacg
      601 ggcatcaagt tccgccatat tgcagccgaa aatgagaaaa ccagcgatct tgccgccgaa
      661 gcagcccgcc gcgcactgga tgcaaccgga ttagacagcg gcgaaatcga tttaattatt
      721 gtggcgacgg caacgccgga tatgcagttt ccttctaccg ccaccatcgt ccaacaaaaa
      781 ctgggcatca ccaacggctg ccccgcgttt gacgtacagg cggtgtgtgc gggctttatg
      841 tacgcgctga ccacggcaaa cgcctacatc aaaagcggca tggcaaaaaa cgcgctggtc
      901 atcggcgcgg aaaccttcag tcgcattgta gactggaacg accgcacaac ctgcgtattg
      961 ttcggcgacg gtgcgggcac ggtggtttta agcgcgtccg acaaaccggg catcatccac
     1021 agcaaactca aagccgacgg caattatctg aaactcttaa acgtccccgg gcaaatcgcc
     1081 tgcggcaaag tttccggttc gccgtacatt tcgatggacg gtcccggcgt gttcaagttt
     1141 gccgtcaaaa tgctgtccaa aatcgccgat gacgttatcg aagaagccgg ttataccgcc
     1201 gctcaaatcg actggatcgt gccgcatcag gcaaaccgcc gcattatcga atcgaccgcg
     1261 aaacatttag gtttgagtat ggacaaagtc gtcctgaccg tccaagacca cggcaacaca
     1321 tccgccgcat cgattccgct ggctttggat acgggcatcc gcagcggaca aatcaaacgc
     1381 ggtcaaaacc tgctgctcga aggcatcggc ggcggtttcg cgtggggcgc ggtattgctg
     1441 caatattgaa taccatgccg tctgaagcag gttttcagac ggtatttccc atatcatgaa
     1501 gcggcaggct ttcttcaaac caattgcgtg tgcggcattt ctgtctgcca tttcgctgcg
     1561 cctccccgta ttgggcgcgt gttacgcaat attgtccctc tatgcgtttg cactttacgg
     1621 catcgacaaa cggcgtgccg tgcggggaaa acgccgcatt cccgaacacc gcctgctgct
     1681 gcctgccttg ttcggcggtt gggcgggcgc atacttgggc agcaggatat tcaggcataa
     1741 aacggcgaaa aagcgttttg ttgtgctgtt ccgtctgact gtttcaggta atgtcctggc
     1801 gaccctcatc ctgattgatt atttcgttcc gcccgaactt tttgtcaaac tcgggcaaca
     1861 tctctgacaa tccaaccaaa caaaggaata ccatgtcttt tgcattcttc tttcccggac
     1921 aaggttctca aagcctcggc atgatgaacg gctttacagg gcaggccgtc gtcaaaaaca
     1981 catttgacga agcatccgcc atattgggtc aagacttgtg ggcgatgatt aacggtagcg
     2041 atgccgaaat catcggtcaa accgtcaata cccaacccat catgctcgcc gccggcgttg
     2101 ccgtttaccg cgcctatttg gaagcgggcg gcaaaacgcc tgccgccgtt gccggacaca
     2161 gcctcggcga atataccgca ctcgttgccg ccggcgcatt ggattttgcc gacgcggtca
     2221 aactcgtgcg cctgcgcgcc gaactgatgc agtctgccgt accgcaaggc gtgggcgcaa
     2281 tggcggcgat tctcggcttg gaagatgaac aggtaaaaca aatttgcgcc gaatccacac
     2341 aaggcgaagt ggttgaagcc gtcaacttca actcgcccgg acaagtcgtg attgcaggca
     2401 ataccgccgc cgtcgagcgc gctatgcttg ccgccaaaga agccggcgcc aaacgcgccc
     2461 tgccgctgcc cgtgtccgta ccttcccatt gcagcctgat gaaacccgcc gccgacaaac
     2521 tcgccgaagc cctgaaaacc gttgaaatca agcagccgca aatccgcgtc atccacaacg
     2581 ccgacgttgc cgcctacgat gatgccgaca aaatcaaaga cgcgctcgtc cgccagcttt
     2641 acagccccgt acgctggaca gaaaccgtca acgcccttgt ttcagacggc attgccgaat
     2701 ccgccgaatg cggcccgggc aaagtgttgg cgggcttggc aaaacgcatc aacaaggccg
     2761 ccgcgtgcag cgcactgacc gatgccggac aggttgccgc ctttatcgaa gcgcactgac
     2821 ttcgttccgc aaaaagcagc ctgccctctt caggctgctt ttcacgtccg aacggcaaac
     2881 agccgcatat ttgcgctata attgatgacg accaaaccac cgacagcggc tgccgttgca
     2941 gttcccgccc taccgatatg atagaaaaac tgactttcgg actgtttaaa aaagaagacg
     3001 cgcgcagctt tatgcgcctg atggcgtacg tccgccccta caaaatccgc atcgttgccg
     3061 ccctgattgc cattttcggc gttgccgcca ccgaaagcta ccttgccgcc ttcatcgccc
     3121 ccctgattaa ccacggcttt tccgcacctg ccgcgccgcc cgagctgtct gccgccgccg
     3181 gcatcatttc caccctgcaa aactggcgcg aacagtttac ctatatggtt tgggggacgg
     3241 aaaacaaaat ctggaccgtc cccctcttcc tcatcatcct cgtcgtcatc cgtggcatct
     3301 gccgctttac cagcacctat ctgatgactt gggtctccgt gatgaccatc agcaaaatcc
     3361 gcaaagatat gtttgccaaa atgctgaccc tttcctcccg ctaccatcag gaaacgccgt
     3421 ccggcaccgt actgatgaat atgctcaacc tgaccgaaca gtcggtcagt aacgccagcg
     3481 acatcttcac cgtcctcacg cgcgacacga tgatcgttac cggcctgacc atcgtcctgc
     3541 tttacctcaa ctggcagctc agcctcatcg ttgtcctgat gttccccctg ctctccctgc
     3601 tctcgcgcta ctaccgcgac cgtctgaagc acgtcatttc cgactcgcaa aaaagcatag
     3661 gcacgatgaa caacgtgatt gccgaaaccc atcagggaca ccgcgtcgtc aagctgttca
     3721 acgggcaggc gcaggcggca aaccggtttg acgcggtcaa ccgcaccatc gtccgcctca
     3781 gcaaaaaaat cacgcaggca acggcggcac attccccgtt cagcgaactg attgcctcga
     3841 ttgccctcgc cgttgtcatc ttcatcgccc tgtggcaaag ccaaaacggc tacaccacca
     3901 tcggcgaatt tatggcattc atcgtcgcga tgctgcaaat gtacgcaccc atcaaaagcc
     3961 ttgccaacat cagcatccct atgcagacga tgttcctcgc cgccgacggc gtatgtgcat
     4021 ttctcgacac cccgcccgaa caggacaaag gcacgctcgc gccgcagcgt gtcgaagggc
     4081 gtatcagctt ccgcaacgtc gatgtcgaat accgttcgga cggcatcaaa gccctcgaca
     4141 acttcaacct cgacatcaga caaggcgaac gcgtcgccct ggtcggacgt tccggcagcg
     4201 gcaaatccac cgtcgtcaac ctgctgcccc gctttgtcga accgtctgcc ggcaacatct
     4261 gcatagacgg tatcgacatc gccgacatca aactcgactg cctgcgcgcc caattcgccc
     4321 tcgtctccca agacgtattc ctgtttgacg acaccctgtt tgaaaacgtc cgatacagcc
     4381 gtcccgacgc aggcgaagcc gaagtcctgt ccgccctcca agccgccaac ctgcaaagcc
     4441 tgattgacag ctccccgctc ggactgcacc agcccatcgg atcgaacggc agcaacttgt
     4501 ccggcggaca gcggcaacgc gtcgccatcg cccgcgccat tttgaaagac gcgccgatat
     4561 tattattgga cgaagccacc agcgcattag acaacgaatc cgaacgcctc gtccaacaag
     4621 cgctcgaacg cctgatggaa aaccgcaccg gcatcatcgt cgcccaccgc ctgaccacca
     4681 tcgaaggggc cgaccgcatc atcgtaatgg acgacggcaa aatcatcgaa caaggcacac
     4741 acgaacaact gatgtcccaa aacggctact acacgatgtt acgcaatatc tcaaacaaag
     4801 atgccgccgt ccggacggca taaacaaaat gccgtccgaa atggtataat cgccccgacc
     4861 ctttcagacg gcactatatc cgccgaccca tccgattatc ttcaatcact gtaaaaccca
     4921 ttatgaccca agacaaaatc ctcatcctcg acttcggttc gcaagttacc cagctgattg
     4981 cccgccgcgt gcgcgaagcc cacgtttact gcgaactgca ttccttcgat atgcctt
//
LOCUS       DAWHXZ010000154         4992 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000154 DAWHXZ010000000
VERSION     DAWHXZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.154"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(224..2500)
                     /locus_tag="WIE35_001617"
     CDS             complement(224..2500)
                     /locus_tag="WIE35_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ4154509.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     NKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSRADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDNAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVYLNRNDYRHDEKAGDAVENFFKNKTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSATSEAVKQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGKRPFIAQADQNAPRVPAARLGVHLKASLTDLIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            2922..3512
                     /locus_tag="WIE35_001618"
     CDS             2922..3512
                     /locus_tag="WIE35_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ4154510.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRDNLPDCLEITAWTDDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3575..4633
                     /gene="trpD"
                     /locus_tag="WIE35_001619"
     CDS             3575..4633
                     /gene="trpD"
                     /locus_tag="WIE35_001619"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ4154511.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQIAQSISQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(4785..>4992)
                     /locus_tag="WIE35_001620"
     CDS             complement(4785..>4992)
                     /locus_tag="WIE35_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ4154512.1"
                     /translation="TVVVPNAQSATLLPIIRKKVKPDGIVYTDTFRSYDVLDISEFSH
                     LRKFNGIPKEHLGLHLKKCQWHFK"
BASE COUNT         1141 a         1150 c         1404 g         1297 t
ORIGIN      
        1 gaattggatt ctcgcctgcg cgggaatgac gggatcttgg gtttctgctt ttgatttttc
       61 tgcttttgcg agaatgacgg cgtgaaagca agaatgatga aacaaagaaa atgggaatga
      121 tggcatagtg gtttgttctt tgtctttgcc atatttccta aaaaattgat taaaaaaggt
      181 tttcagaatg ccgtctgaaa accttttttc tgcctgtccg attttaaaac ttcacgttca
      241 cgccgccggt aaagctgcgg cccatttgcg gcgtatcaga gagaaagctg ctgtgggcgt
      301 aaacggattg gttgagcagg ttgtcggctt tgacgtacca attccactcg ccatagcgcg
      361 tattgcggcg gtagtttgcg ccgaggttga gcatatggtg tccgggcgta cgcgtttcgt
      421 agcgggcgag tttgttttgg gcgaacacgc ggtagtagtc caagttggca tcgatgaggt
      481 cggtcagcga ggctttcagg tgaacgccga ggcgcgcagc cggaacgcgc ggcgcgtttt
      541 ggtcggcttg tgcgatgaag ggacgcttgc cgtaagcatc ttccctgccg ggtagggaag
      601 gcaggttttt cagacggcct cgtacatagt cgccggaaac accgatgcgg tagcgcggtg
      661 tcggtttgaa gtagatttca ccttccgcgc cgtagaagtc tgcgccggat tggttgtagc
      721 gcacgagctt catttcgctg tcgtcttcga tggatttggg gccgcgtccg tcgtttaagg
      781 tttgggcgta aatgtagttg ccgaagcggt tgcggtagag tgccagattg tattgccagc
      841 ggtcgccttc gtagcccaac gcgagttcga tgttgttgga acgctctttg ttcaggtgtt
      901 tgttgccgac ttcaaaagtg ttggtggcaa cgtgtttgcc gtgtgcgtac agctcttgcg
      961 ttgacggcag gcgttcctga tgggaggcgg tcaggctgag tttgtgttgc ggcgtgaaat
     1021 accagttgcc cgaaagtgcg aacgagcggg cggtttggcg gtgcgcgccg aggtcgggca
     1081 gggggtggtt gtagtagttt tcccgatcaa tcaatgcttt gtcgtaccgg atggaggctt
     1141 tttgtttttc cacgcgtacg ccgccttcaa gcgtgaagtt gtcccagttt gcctgttcta
     1201 caccgaaaaa gctgtaatgg cgcacattgt tgtcaatcaa catcggttgt ttgaccgctt
     1261 cggatgtggc agataaagca ctggattttt gttgtaaata ttgcacgccc cagctgcctt
     1321 tcagacggcc tatgggttgg tggcgcaact cgatgcgggc gttttgcgtt ttgtttttga
     1381 agaagttttc cactgcatcg cctgcttttt cgtcgtggcg gtagtcgttg cggttcaagt
     1441 atacgcgcag ggcttcaaaa ccggggaacg gttgcttcca ttcggcacgg agttcgtagc
     1501 gtttgttgcg caggtctatc cacggtctgc cgctgtgggt gtgtgcgtgt gcattatcgt
     1561 cgtcgtggaa gccgcagctc aagcccggat tgtcgtaatc gatgtcttct tcggtcaaca
     1621 ggtgcggata aagctgtaaa tagcgtttgt taatcaagct cttttgccag atgatgtcgg
     1681 cgtggcaatc atcgtattcg tggctgtggg caggcagacc atattggtcg cgacggtcgc
     1741 tgtacgctac gccgataaaa cctttttcgc caacccaaga cagcccgatg ctgcccgttt
     1801 gcgaatcggc gcggctgtcg ggcaggcgtt tcagattgcg gtaacgcggt acggcgtaat
     1861 cgcccgattt gcggtacaag ccttccgtat gcaatacaaa gtttttgccc aagccgatat
     1921 tgatgcctgc ggatgtcagt ttttccaaat tgccgctgct caaacgcaat ccggcttcgc
     1981 ccgatacgcc gttttcaggc attttttcgg gaattttatt gtcggcaaca tcaaccagcc
     2041 ccgcgacatt gcccgaacta tataaaagcg taaccgggcc gcgcaggatt tcgacctgtt
     2101 gcgacaaggc ggtatctacc ataatggcgt gatcgggcga aaaatccgcc atatcgcccg
     2161 tttcgccgtg atggttcaaa accttaatcc gcctgcctgt ttgaccgcgt atcaccggtg
     2221 cggacgcgcc gccgccgtat tgcgaggcgt gaatacccgg tacgccgtcc aaagcatcgc
     2281 ctaagttgac ggctttttgg cgcaaggtat cgccggagat gattttgtcg gaggcggtcg
     2341 aagtgtgcag cagccccgaa gtggcgcgcg gacggctttt gccgacgacg ctgaccgttt
     2401 ccaaatccac cgattgctca gtttcatgcg cttgggcgag gaggggtgtg ttgattaaaa
     2461 gaattgataa aacaatgggt ttgagtgtag tttgtgccat tttggtttct cgtcgcattt
     2521 caaaagtttg ttattatata acattacatt ccttatatca taagattttg agaacactca
     2581 gagggcatag gcaaaagttt ttcaaatgaa acggttgcgg catcgggcgg tgtccatttg
     2641 tatccgccgt ccttcggggg cgcggttgat gttgacgcag attccgctgt gtttgcccat
     2701 tatgttcggc tgcgggataa ccaaaattta tgagtgcata aaaacggtac gttcccgaac
     2761 ggtcgaaaag gtggcaaaat ggcgtacttg tcagaacgcg aggctctgcg ccaggttcac
     2821 gagggtgcat cgggcacgct aagatataag agggtatgga tgggggtatc ggaaatgcag
     2881 ttaataacaa acaaattata aatcaatagg ttaataacaa aatgcttttg tttatcgaca
     2941 attacgacag ttttacttac aacatcgtcc agtatttcgc tgaattgggg caggaagtcg
     3001 ccgtgcgccg caacgatgat attacgttgg aagaaatcga ggcattgaat ccgcaatatc
     3061 tcgttatcgg acccggtccg tgttcgccca aagaagcggg gatttccgtg gcggcgatgc
     3121 gccattttgc cggccggctg ccgattatgg gcgtgtgcct cgggcatcag acgataggcg
     3181 aggcgttcgg cggcaggata gtccgcgcca aaacgctgat gcacggtaag gtgtcgcccg
     3241 tgtcccattc gggcaagggt atgtttaaag gtttgcccaa tccggttacc tgtacgcgtt
     3301 atcacagcct cgttatcgat cgggataatt tgcccgattg tttggaaatc acggcttgga
     3361 ctgacgacgg cgagattatg ggcgtgcgcc ataaggaata tgccgtcgag ggcgtgcagt
     3421 tccaccccga agccctcttg accgaacacg gacatgatat gttgaacaat tttttaatcg
     3481 aatttcaaaa cttcaaaccg caaaaaatct gacgcgatgc cgtctgaagg cttcagacgg
     3541 cattttcgtc cgaatattga acggaggaca aaaaatgatt acaccgcaac aggctatcga
     3601 acgattaatc agcaataacg agttgtttta cgatgaaatg accgacttga tgcgtcagat
     3661 tatgagagga caggttctgc cggagcagat agcggccatt ttgacaggat tgcgtatcaa
     3721 ggttgaaacc gtttccgaaa ttaccgcagc tgcagccgtc atgcgcgagt ttgcgacaaa
     3781 agtgccgctg gagaatgcag aggggctggt cgatatcgtc ggtacgggcg gggatggtgc
     3841 caaaaccttc aatatttcga cgacctcgat gtttgttgcc gcagcggcag gcgcgaaggt
     3901 tgccaaacac ggcgggcgtt ccgtttcatc ttccagcggc gcggcggacg tggtggagca
     3961 gatgggcgca aacctcaacc tgactcccga acagattgct caaagtatca gccagaccgg
     4021 tatagggttt atgttcgccc ccaatcacca cagtgccatg cgccatgtcg ccccggtacg
     4081 ccgttcgctc ggtttccgaa gcattttcaa catattgggt ccgttgacca atcctgcggg
     4141 cgcgccgaac caacttttgg gcgtgttcca caccgatttg tgcggcattt tgtcgcgggt
     4201 cttgcaacaa cttggttcaa aacacgtttt ggtcgtttgc ggggagggcg gcttggatga
     4261 aattacactg acgggcaaaa cgcgcgttgc cgaactcaaa gacggaaaaa tcagcgaata
     4321 cgacatccgc ccagaagatt tcggtatcga aacccgccgc aatttggatg aaatcaaagt
     4381 tgccaatact caggaatctt tgttgaaaat gaatgaggtg ctggatggga aagaaggggc
     4441 tgcgcgcgat atcgtattgc ttaataccgc cgccgcccta tatgccggaa atgtcgccgc
     4501 ttcgctttca gacggcatat ctgccgcacg ggaagccatc gattcaggta gggcaaaatc
     4561 gaaaaaagag gagtttgtcg gttttcaacc acaacaaaga tgccattttc ttggaaagat
     4621 ggagcttggg tgatgccgcc atgattatgg aacttttgtg gcaaaacata agcacttcac
     4681 gaagagaact taccaaactg tttttatata aaaacttggg gctgtactag ataaccagac
     4741 caaattccca ttaactaatt gtcttaaaat ctgaatttga gattctattt aaaatgccat
     4801 tggcatttct ttaaatgcag ccccaaatgc tctttgggaa tgccgttaaa cttacgtaaa
     4861 tggctaaatt cactaatatc aagcacatca taactacgaa aggtatccgt gtatacaatg
     4921 ccatcaggct taactttctt tcggataatt ggcaacaatg tcgctgattg cgcattagga
     4981 acaacgacgg ta
//
LOCUS       DAWHXZ010000155         4977 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000155 DAWHXZ010000000
VERSION     DAWHXZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.155"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(265..2301)
                     /locus_tag="WIE35_001621"
     CDS             complement(265..2301)
                     /locus_tag="WIE35_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ4154513.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVERLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAYLVCNFAPPVG
                     DKEARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YDVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRGAAESFKAFRGREPRIDALLRHSGFDNAA"
     gene            complement(2389..3261)
                     /locus_tag="WIE35_001622"
     CDS             complement(2389..3261)
                     /locus_tag="WIE35_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ4154514.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAVVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            3531..>4977
                     /locus_tag="WIE35_001623"
     CDS             3531..>4977
                     /locus_tag="WIE35_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEZ4154515.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKV
                     VGDSDKKGTTVRFLASAETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIG"
BASE COUNT         1081 a         1312 c         1470 g         1114 t
ORIGIN      
        1 gcgttgtttg accgtttcag acggcatttt tatcaaattc aaaaatattc cgtcattccc
       61 gcgaaagcgg gaatctagaa cgtaaaatct aaagaaaccg ttttatccga taagtttccg
      121 caccgacaga cctggattcc cgcctgcgcg ggaatgacgg gattttggct tactgttttt
      181 gtgaaaatgg cgaaaccgtg caaatgctga tgccgtctga aacactgttt gattgtttca
      241 gacggcattt tttgttcaca tcgatcaagc cgcgttgtcg aaaccgctgt ggcgcaagag
      301 tgcgtctatg cgcggttcgc gtccgcggaa ggctttgaag gattctgccg cgccgcgcga
      361 tccgccgacg gcgaggattt cctgccaaaa gcgtttgcct gtggcggcga catcgtcgct
      421 ttcttcaaag gcggcgtatg cgtccgcact caatacttcc gcccacgcgt agctgtaata
      481 gcctgcggaa tagccgcctg cgaagatgtg gccgaagctc aaggcgaagc ggttgtattc
      541 gggcggttgg atgacggcga cttttttgcg cacgctgtct aaaacctgtt gccagttttt
      601 cagacggcct tcgtcgtctt cgctgtaaat catcatatcg aagagggcaa actccatttg
      661 gcggacgagg aacataccgc gttggaagtt tttggcggcg agcattttgt cgaagagttc
      721 tttcggcagg ggaacgccgg tttcttcgtg ggctgacatt tgtgccaaga cgtcgtattc
      781 ccagacgaaa ttttccataa actggctggg cagttcgacc gcgtcccatt ctacgccgtt
      841 gatgccggat acgcccagtt cgtccacttg ggtaagcagg tggtgcagcc cgtgtccggt
      901 ttcgtggaag aggatgagga tttcgtcgtg gctcaagcgg gcttctttgt cgccgacggg
      961 cggggcgaag ttgcagacga ggtaggcggt gggcagttgc agcgtgccgt cggcgaagcg
     1021 gcggcggcct ttgtagtcgt tcatccacgc gccgccgcgt ttgccttcgc gtgcgtacaa
     1081 atccatataa acgccgccta tggtttcgcc gttttgttgc aattcaaaat agcgcacgtc
     1141 tttgtgccag acggggacgg ttttttcggt aaatccgatg ccgtagagtt ttttgatttg
     1201 ggcgaacagt ccgtttaata ctttgccgac ggggaagtat tttttgactt cggtttcgct
     1261 gaacgcgtat ttggcttcgc gcagtttttc gctggcgtag cccaagtccc acggttgcaa
     1321 atcggcgagg ttcaggcttt cgcgggcgaa ggctttgact tcggcgaggt ctttttcggc
     1381 gtagggtttg gcgcggcggg cgaggtcgtg caggaagttt aaaacttgtt cgggcgtgtc
     1441 cgccattttg gttgccagcg acaattcggc gtagtttttg aagccgagca gtttggcggt
     1501 ttgcagggcg tttgcgagcg tgcggtcgat gttggcggtg ttgtcgaatt tgccgtcgtc
     1561 tgaaagttcg ctggcgcggg taacgtaggc gcggtagatt tgttcgcgca gttcgcggtt
     1621 gtcggcgtat tggatgacgg cgaggtagtg cggaatctgc aaaccgattt tgtagcctgt
     1681 tttgccttcg ctttgcgcgg cggcggcaaa catggcgagc gcgtcttcgg gaatgccggc
     1741 aagcggtgcg gcatcgtcaa agtaaatgcc gaacgcgtcg gtcgcgtcta ggacgttttg
     1801 ggagaatttg gcggaaagtt gcgcgccttc ggtttgcagt tttgccagtt ctgcctgctg
     1861 ttcgggcggc agttccgcgc cgctgaggac gaaatcgcgc agatcgtggt tgagtttggt
     1921 tttttgcgcg ggggagaggg tgtcgaactc gggggagttt ttgatggttt tgaagcggtt
     1981 gtacagctcg atgtcttgtc cgatttcggt gaagaagacg gtgatttcgg gcatcagttc
     2041 gttatagacg gcgcgcagtt cgggcgtgtc ggcgacggag ttgaggtgcg acaccacgcc
     2101 ccaaatcctg ccgacgcgct cggtgatgcc ggtcagacgc tcgacggtgt tcgcccagcc
     2161 ggtgtgcgtt tgggctttga tggcggcgat ttgttcgcgc gcttcggcaa tggcggtttg
     2221 cagggcgggt ttgatgtctt cggttttgat ttgatcaaaa cggggttctt cgcccaaatg
     2281 gagcagtgcg ttgtcagtca taagatgggt ttcctttgcg tgttgtgctt cagacagcat
     2341 atcggcaata tgccgtctga agccggaaat ggggtcggtc ggggaaagtt aaagtgtttc
     2401 ggagccggcg gggtaattta acaggcggta ttgtacgcgc aaaaattcgt ccataaccgc
     2461 ctgttggatt tccagccgct ttgaaacggg ggaggcgaag cggacgatga tgcggtatgc
     2521 cttgtcatcg tacggcacgc gggtaacgcg cggtctggcg gcgggcgtga taaacagttt
     2581 ttccgcctgc acgttttcca aatgccgttg gatggcgggg atgtagggcg cgcacaaggg
     2641 ctcgagtacg gctttcagac ggcatacggc ttcatccgaa tccaaatgga tggggacggg
     2701 gatttcgacc gtatggatga cgtagtcgcc caaaatattg tcgcggcgca cgggatggct
     2761 caacaacagg ctgttgggga aagaaacggt ggttcccgca agctgtccga ccagggggtt
     2821 cggaccgacc tgcatcatca gcgtgttcaa caggttgatg tcgaccacgc gcccgcgcag
     2881 gccgttgatt tcgatatagt cgccgaccga gtattgctgg gtggcagacc ttaaaatact
     2941 gcccgacaga cacataatca gttcctttgt cgccacgacg accgccgccg ccaccgcaaa
     3001 catcgacaaa gccagcgttt ggatttgcgc cgaccagata aatgccagcg aaaacagcac
     3061 caaaagcagc gttatattgc ggctggcaac caaaaaccgc cgcttgcttt cgatgccgaa
     3121 atccggatgc cgtttgaagt ggatattcaa cagaagggcg cgcgccagca gcaaagccgc
     3181 gaccaccgcc acggattcga ccgcctccgc acgtatcggg acggcaccga gccaagtgtc
     3241 caacatattc catatttcca tttccgcgcc cctgtccgaa aagtataggg tgtagatttt
     3301 agtggcaaaa aaacgctttt gccactttac gggacaatcc ccgacctgat gccggaggca
     3361 aacgtaccgt ttcatctttt cccggcggca cactgatgcg gataacgctg aattaggcgg
     3421 caaaatcggc tataatacac aattatatat gccgtcccgc ccgctttttc tacaaggtgc
     3481 ggacttgttt tggcggcatc gcaaaatctt ttcaaaatcc ggcaaaaaat atgactgaac
     3541 aaaaacacga agaatacggc gccgacagca ttcaggtgct cgaaggcttg gaagcggtac
     3601 gcaaacgccc cggtatgtac atcggcgaca cgcaggacgg cagcggcctg caccacatgg
     3661 tgttcgaagt attggacaac gccattgacg aagcactcgc cggacattgc gacaaaatca
     3721 ccgttaccat ccacgccgac cattccgtca gcgtcgccga caacgggcgc ggtatgccca
     3781 ccggcatcca cccgaaagaa ggacgctccg ccgccgaagt catcatgacc gtattgcacg
     3841 cgggcggtaa attcgacaac aacagctaca aaatctccgg cggcctgcac ggcgtgggcg
     3901 tgtccgtcgt caacgcgctg tccgactggg taacgctgac catctaccgc gacggcaaag
     3961 aacacttcgt ccgcttcgtg cgcggcgaaa ccgaagagcc gttgaaggtt gtcggcgatt
     4021 ccgataaaaa aggcacgacc gtgcgcttcc tcgccagtgc ggaaaccttc ggcaacgtcg
     4081 aatacagctt cgacatcctc gccaaacgca tccgcgaact ttcgttcctg aacaacggcg
     4141 tggacatcga attgaccgac gagcgcgacg gcaaacacga aagcttcgcc ctttccggcg
     4201 gcgtggctgg attcgtgcaa tacatgaacc gcaaaaaaac gcccttgcac gaaaaaatct
     4261 tctacgcgtt cggcgagaaa gacggcatga gcgtcgaatg cgcaatgcaa tggaacgaca
     4321 gctatcagga aagcgtgcag tgcttcacca acaacatccc tcagcgcgac ggcggtacgc
     4381 acctgaccgc gttgcgccaa gtgatgacgc gcaccatcaa cagctacatc gaagccaacg
     4441 aagtcgccaa aaaagccaaa gtggaaaccg ccggcgacga tatgcgcgaa ggtttgacct
     4501 gcgtgttgtc cgtcaaactg cccgacccca aattttcgtc ccaaaccaaa gacaaactgg
     4561 tttccggcga aatcggtccc gtcgtcaacg aagtcatcag ccaagccctg accgacttcc
     4621 tcgaagaaaa ccctgccgaa gccaaaatca tcaccggcaa aatcgtcgat gccgcccgcg
     4681 cgcgcgaagc cgcccgcaaa gcccgcgaaa tcacccgccg caaaggcgta atggacggct
     4741 tggggctacc cggtaaactc gccgactgcc aagaaaaaga ccccgcattg tcagaacttt
     4801 atctggtcga gggcgattct gcaggcggtt ccgccatgca ggggcgcgac cgcaaattcc
     4861 aagcgatttt gccgcttaag ggcaagattt tgaacgtcga aaaagcgcgc tttgaaaaaa
     4921 tgctcgccag tcaggaagta gccgctttga ttaccgcgtt gggcgcgggc atcggca
//
LOCUS       DAWHXZ010000156         4953 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000156 DAWHXZ010000000
VERSION     DAWHXZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.156"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(189..1514)
                     /locus_tag="WIE35_001624"
     CDS             complement(189..1514)
                     /locus_tag="WIE35_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ4154516.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPAPAGTTVAGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMQQNPQRLAEVLGQNPSYIFF
                     RELTGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1726..2565
                     /locus_tag="WIE35_001625"
     CDS             1726..2565
                     /locus_tag="WIE35_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ4154517.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGIGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKQQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            2562..3728
                     /locus_tag="WIE35_001626"
     CDS             2562..3728
                     /locus_tag="WIE35_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ4154518.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRIHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3992..>4953
                     /gene="efeB"
                     /locus_tag="WIE35_001627"
     CDS             3992..>4953
                     /gene="efeB"
                     /locus_tag="WIE35_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ4154519.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKRGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNT"
BASE COUNT         1105 a         1306 c         1461 g         1081 t
ORIGIN      
        1 aagcaccaag tgaatcggtt ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc
       61 tgatttaaat ttgatccact ataattccgt caaataagaa aggaattttg tgcctgcggt
      121 atcgcaaaac ttcgccttaa tgcgcccgat tgcctaggga tgggcttcag acggcattgt
      181 tttccggttt acgggcggta ttcgggcttc ataccgttgg gcagaagctg ccagacatat
      241 cccgtggttt tctgtttgcc ggcaagttcg ccggcttcgt cgccgtaacc ccaaaaataa
      301 tccacgcgca ccgcgccttt aatcgcgctg ccggtatcct gcgccataat caggcggttg
      361 agggctttgc gggtaaccgg atgggcggtg gcgacaaata agggcgcgcc caaggtaatg
      421 tagtgccggt cgactgcgcc ggcgtactcg cccatcagcg gcgtgcccag tgcgccgaca
      481 gggccgtcat tgctgcttcc ggtaagctct cggaaaaaga tatagctggg gttttgcccc
      541 aaaacttcgg cgaggcgttg cgggttttgc tgcatatagg ctttgatgcc ctgcatcgag
      601 gtctgcccga gcttgaggta gcctttgtcc gccatatagc gtccgatgga aacgtaggga
      661 tgttcgtttt tgtcggcata gccgatgcgg atgtatttgc cggacggggt tttcagacgg
      721 cccgagcctt ggatgtgcat aaaaaaaagt tcgacggggt cttcggcgta accgagtatc
      781 ggggctttgc cgtcaagcgc gccgccgttg atttggttgc gcgtgtggta ggggaggaag
      841 cggcttcctt caaacctgcc tttgattgcc gttgtgcgcg cggtgatggg gaatcgggag
      901 aggtcggcgg tgtgcgtgcc gccggcattg tcgattgtgc cgctgttttt ccccgtctgc
      961 ctgatgcgga caagggcttt tccgctccgc aaaccggcag gcagggggac ggagataaaa
     1021 tcgtcgggaa taccgtaaat cgggaagcgg gcttgtgccg tccgcctgtc gtcgcccttc
     1081 agcaccggct cgtaatagcc ggtaaccgta ccggcaaggc ttccgttgcc tgcaacctgc
     1141 cacggcgtga aatagcgttc aaaaaactgt tttgcctgaa aggaatggac gggggtttga
     1201 aaggcttggg cacacacatc ctgccagcct tggcggtttt tcaaattggc gcagccgagg
     1261 cggaaggatt gcaggctttt ggcgaaatcc tgcgccgccc agtggggcag ggacaggtgc
     1321 ggcacaacgg tataaacggc cccgccgccg gcaaccgtcg ttccggcggg ggcggggatg
     1381 ccgaccggcc ggtccgggcc gttgatgacg gatgtgtcgg gttgcggaaa ggtttggatg
     1441 ctcttgcttt ggcaggcggc gaggatggcg gcggcgatgc cgcacagggc ggcgcggaac
     1501 aggtattttt tcataatgga caatgttgtc agcagtaata aaaaaagatg gtttcgggcg
     1561 gcgttgcggc agccgtggag aggggatttt aacacagggc gcagctgcag cctgcggaac
     1621 tttccgccgc gcggtactgc agataaaaat aacttgcatt tgtatttaca agcaatgaaa
     1681 atattccgat aatatattat tcatcatcct tgttcgttcg cgtttatgct ggtcgctttt
     1741 ttaattatgt tgcgcgaggg tatcgaagcc gcgctcatcg tcggcatcgt tgccggtttt
     1801 ctgaaacagt ccggacattc caaactgatg cctaaggtct ggttcggggt cgtccttgct
     1861 tctttgatgt gtttgggcat cggatacggc atccattcgg caacgggcga gattccccag
     1921 aagcagcagg agttcgtcgt cggcattatc ggtttggttg ctgtcgcgat gctgacttat
     1981 atgattttat ggatgaaaaa ggcggcgcgt tcgatgaagc agcagcttca ggattctgta
     2041 caggcggctt tgaaccgtgg cagcggtcaa ggatgggcct tggtcggtat ggcgtttctt
     2101 gccgtggcgc gtgaaggtct ggagagtgtt tttttcctgc ttgccgtatt caaacagagc
     2161 ccgacttggc agatgccggc cggcgcggta gcgggggttt tggctgccgc cgtgattggc
     2221 gcgttgattt atcagggtgg gatgcgcctg aatctggcga agtttttccg ttggacgggg
     2281 gcgtttctga ttgtcgttgc cgccggcctg cttgccggtt cgctgcgcgc gctgcatgag
     2341 gcaggtattt ggaacgcgct tcaggacatt gtgttcgact catcaaaata tttgcacgaa
     2401 gacagcccgt tgggcgtgct gctcggcgga tttttcggct ataccgacca tccgacgcag
     2461 ggcgaagcct tggtttggct gctgtacctt attcccgtca tgacttggtt tttgtgcggc
     2521 agcaggccgt ctgaaacttt aacccgtaaa gaggagctga aatgagaaaa ttcaatttga
     2581 ccgcattgtc cgtgatgctt gccttaggtt tgaccgcgtg ccagccgccg gaggcggaga
     2641 aagctgcgcc ggcagcgtcc ggtgaggcgc aaaccgccaa cgagggcggt tcggtcagta
     2701 tcgccgtcaa cgacaatgcc tgcgaaccga tggaactgac cgtgccgagc ggacaggttg
     2761 tgttcaatat taaaaacaac agcggccgca agctcgaatg ggaaatcctg aaaggcgtga
     2821 tggtggtgga cgagcgcgaa aacatcgccc ccggactttc cgataaaatg accgtcaccc
     2881 tgttgccggg cgaatacgaa atgacttgcg gtcttttgac caatccgcgc ggcaagctgg
     2941 tggtaaccga cagcggcttt aaagacaccg ccaacgaagc ggatttggaa aaactgtccc
     3001 aaccgctcgc cgactataaa gcctacgttc aaggcgaggt taaagagctg gtggcgaaaa
     3061 ccaaaacctt taccgaagcc gtcaaagcag gcgacattga aaaggcgaaa tccctgtttg
     3121 ccgacacccg catccattac gaacgcatcg aaccgattgc cgagcttttc agcgaactcg
     3181 accccgtcat cgatgcgcgt gaagacgact tcaaagacgg cgcgaaagat gccggattta
     3241 ccggcttcca tcgtatcgaa tacgcccttt gggtggaaaa agacgtgtcc ggcgtgaagg
     3301 aaattgcagc gaaactgatg accgatgtcg aagccctgca aaaagaaatc gacgcattgg
     3361 cgtttcctcc gggcaaggtg gtcggcggcg cgtccgaact gattgaagaa gtggcgggca
     3421 gtaaaatcag cggcgaagaa gaccggtaca gccacaccga tttgagcgat ttccaagcca
     3481 atgtggacgg ttcgaaaaaa atcgtcgatt tgttccgtcc gctgatcgag gccaaaaaca
     3541 aagccttgtt ggaaaaaacc gataccaact tcaaacaggt caacgaaatt ctggcgaaat
     3601 accggactaa agacggtttt gaaacctacg acaagctggg cgaagccgac cgcaaagcgt
     3661 tacaggcctc tattaacgcg cttgccgaag accttgccca acttcgcggc atactcggct
     3721 tgaaataagc cgcaagcgtt cagacggtat ttggcggcag ataccgtctg aagttttata
     3781 gtggattaac aaaaaccagt acggcattgc ctcgccttgc cttgccgtac tatttatact
     3841 gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actatatccg ccatatattg
     3901 cagggcggga tttcaacctg ccgctatcgg ttaatggaaa aacggcgtgc agggataccc
     3961 atcctgctgc acggatattg aaggaaacac catgagcaaa aaccaacccg cacaaccgac
     4021 caggcgcact ctttttaaaa ccgcgatcgc agccggagca gtcggcgcaa tcggaggtta
     4081 tctcggcggc aaaaaacggg gcgaaaccgc cgaacgcacc gccgaaagtc aacactcgcc
     4141 ccaagcctat ccctgctacg gcgaacatca ggcaggcatc gttacgccgc agcaggcgtt
     4201 ttcgattatg tgcgccttcg acgtaaccgc gcaaagtgcc aagcagctgg aaaacctgtt
     4261 ccgcacgctg accgcccgca tcgagtttct cacccaaggc ggcgaatacc aagacggcga
     4321 cgacaaactt ccgccagccg gcagcggcat tttgggcaaa gccttcaacc ccgacgggtt
     4381 gaccgttacc gtgggggtgg gcagcagcct gtttgacggc cggttcggac tcaaagacaa
     4441 aaaaccgatt catttgcagg aaatgcgcga cttctccaac gataagctgc aaaaaagctg
     4501 gtgcgacggc gatttgagcc tgcaaatctg tgccttcacc cccgaaacct gccaagccgc
     4561 cctgcgcgac atcatcaaac acaccgtcca aaccgccgtt atccgctgga gtatcgacgg
     4621 gtggcagcct aaatccgaac ccggcgcgat ggcggcgcgc aacctgttgg gcttccgcga
     4681 cggcacgggc aaccccaaag tttccgaccc caaaaccgcc gacgaggttt tgtggacggg
     4741 tgtggccgcc aacagcctcg acgaaccgga gtgggcgaaa aacggcagct atcaggcagt
     4801 ccgccttatc cgccactttg tcgagttttg ggacaggacg ccgcttcaag agcaaaccga
     4861 cattttcggg cggcgcaaat acagcggtgc gccgatggac ggcaaaaaag aagccgacca
     4921 accggatttt gccaaagacc ccgaggggaa tac
//
LOCUS       DAWHXZ010000157         4906 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000157 DAWHXZ010000000
VERSION     DAWHXZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.157"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(458..802)
                     /locus_tag="WIE35_001628"
     CDS             complement(458..802)
                     /locus_tag="WIE35_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154520.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(840..1163)
                     /locus_tag="WIE35_001629"
     CDS             complement(840..1163)
                     /locus_tag="WIE35_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154521.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYETFDIL
                     KQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(1166..1354)
                     /locus_tag="WIE35_001630"
     CDS             complement(1166..1354)
                     /locus_tag="WIE35_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ4154522.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(1424..1759)
                     /locus_tag="WIE35_001631"
                     /pseudo
     CDS             complement(1424..1759)
                     /locus_tag="WIE35_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(1865..2236)
                     /locus_tag="WIE35_001632"
     CDS             complement(1865..2236)
                     /locus_tag="WIE35_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ4154523.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(2233..2484)
                     /locus_tag="WIE35_001633"
     CDS             complement(2233..2484)
                     /locus_tag="WIE35_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154524.1"
                     /translation="MDNTRIMAAHEAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            complement(2616..2822)
                     /locus_tag="WIE35_001634"
     CDS             complement(2616..2822)
                     /locus_tag="WIE35_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154525.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(2832..3677)
                     /gene="mafB"
                     /locus_tag="WIE35_001635"
                     /pseudo
     CDS             complement(2832..3677)
                     /gene="mafB"
                     /locus_tag="WIE35_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(3774..3986)
                     /locus_tag="WIE35_001636"
     CDS             complement(3774..3986)
                     /locus_tag="WIE35_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154526.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            complement(4063..4422)
                     /locus_tag="WIE35_001637"
     CDS             complement(4063..4422)
                     /locus_tag="WIE35_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ4154527.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(4427..>4906)
                     /locus_tag="WIE35_001638"
     CDS             complement(4427..>4906)
                     /locus_tag="WIE35_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ4154528.1"
                     /translation="KWDWVKNTDYKTPAARPMQTLDGEMAGGNKPPKSITSGGKANAA
                     TYPQLVNQLTGQNLKNIAAQDPRLSLALHKSEKNFPIGTATYEEADRLGKIWVGEGAR
                     QTSGGGWLSIDGTRQYRPPTEKNSQFATTGIQANFETYTIDSNGKRNKIKNGHLNIR"
BASE COUNT         1360 a          960 c          777 g         1809 t
ORIGIN      
        1 tcattaatac cttgtaaagt ttgctgcgca gagatgtagg tcggatattt gtatccgaca
       61 aaaatatttg acgcatctat cgtttccgaa atgccgctgt tggaaaatgt cggattcgag
      121 aatccgacct acggctattt cgtttaagtc ataaatagcc attcatgttc cttctgaatt
      181 tgcttttgct atccttagaa ccaatcatat aaacattatc atcattaatt aatgttctaa
      241 gtttatgaag cggtcaaatc tgtcaaacta ccagagatga agcatatcct cctggaagta
      301 attctgttcg tgctgtttcc atatcttctt tgccatcatc agttacttct ctgtctaaat
      361 aaaatcttac gataagtttt tgtctaattt catcaaattc tgccgtaatt aatcttatat
      421 tgatatgaat acacggttca tacctgctat tagccagtca ggtgtagttg gatggggatt
      481 catttttaat aatatcttca agcaagtgaa atgtaagaaa agcgtagata cttgaaggcc
      541 ggctatctga ttggcaaaaa gaaatttcgg agataaagaa tttccgttta tatttttcat
      601 aaattaaatc aacaccttgt tttacataaa aactaacttc ctcaggaagg atatttataa
      661 cattctctcc ttttactttt atagttaatg gtttgatttt tatgttttca tgaatatctg
      721 ataggattat ttctaaaaaa ttatttttat ttccagtcat tctggctatt ttatgtatat
      781 ttccattgtg ttcgattatc attttatttt ttccaaggat ttccttctat attgatagtt
      841 tatctaaaga tttcatttaa aatatcctga ctaatttctt gtttatataa ttctacaatt
      901 ttctgtttct gtgtatttgt ataaaatttt tcgttggtat ctgcttgata ttttaattgt
      961 ctcaatattt ccatgatttc atattctgat ttttcatcat attcttcaat catgtaatca
     1021 acaaattgtt ttagaatatc aaaggtttcg taactttcca aaattttaat atgtttatcg
     1081 gcaaaattgg acatatacca ttcgtcagtg attggattag tatttaaatc atctaaaaaa
     1141 acatttacat catctaaaaa catgactaat ccttaaactt aaaatctgtt ttctcaccgg
     1201 ttcttgagtt ttcaatgtaa tgtatttcaa ttttgtttac gttttgaaca cgcttaaccc
     1261 aaccgtcttt tgctaaccaa ccatttttag tatcggacat aggaggtatt ctcgcaggag
     1321 ttttgccctg tgggtttgcc ttaacttctt ccattgctaa ttgttccttt aaattattag
     1381 gtattgtacg accggttgat ttttcaacag attgtgtccc tatttttaat gatttgtttc
     1441 ccccagccat ttccccgtct acagtctgca tagggcgggc agcaggtttt ttatagccgg
     1501 tatttttaac ccaatcccat ttagtcgggt taagttctac ttttttaccg cgccaaaccg
     1561 tacctgcggc ctctgctacg gaaagggcag tttgggctgt tgcagttata ttcggatggg
     1621 catcagccca ttgtctggcg gaattaatgc cgttttttac cgcaaaagca ctgtcctgta
     1681 ataggctcgc ggcagcaagt tgtgcttccg gacttaaatt tcctaattca ttaatatctt
     1741 gtaaagtttg ctgcgcagag atgtaggtcg gatatttgta tccgacaaaa atatttgacg
     1801 cgtctatcgt ttccgaaatg ccgctgttgg aaaatgtcgg attcgagaat ccgacctacg
     1861 gctgctacca accatcattt tcagcttctt ttagccaagt atcaaaattt tcaaaagtct
     1921 cgtaatattt tgtattatct aaattaacta ctataatttt aggattacct gatttatctg
     1981 aaatatcaaa acaggcagcc cactcttggt ttctagcaaa aggtaatagg tttggaatgc
     2041 cagttatttc aaaataaatg tcaattactt ctgatatatt gctttctgca ttttcaaacc
     2101 accaaggaaa agaatattgt tcatcgtagt taatggcatg agtggattga gctaatttta
     2161 aataactttg cggatattta aatcctttag gaagcattgc ttcaggatac ggaacaaaac
     2221 tattttgttt tatcatttcc taagtacctt tacatcataa atacttccgt taggaaatct
     2281 tttgctccac ccttctggaa ctcctttttg tgtttcttgc tttctaattc gtagctggat
     2341 agcttctccc caagtttgag gctcaatacc atcatgctta aacctgattc tctcttttga
     2401 tgataatcgg tcattaaaat tatgaacatt cgcttccact tttacccctg cttcatgtgc
     2461 tgccataata cgcgtgttat ccatactagt aggtgctcca tcaggcatat taacaacatc
     2521 aaccctgtcg ccaacccatg atttttttcg ttcattgcag ctacaagaga atcatagttg
     2581 taatttgcac taggctctga caaaaatttg ggttgttaag ttgtcatttt tttataaatt
     2641 ccagcaaact gttgttgtaa attatctact tcatgttttc taacaccaga tacattttca
     2701 aattctcttg aatctacaca tacgcccgca tatatctcat tcaagatagc tcgtaaaaca
     2761 aaaacttcat cttgagttag tgaaatagtt gagttttgat tatttatgga tagtatattc
     2821 atggcttttt cctataaatc atcataatat tttttacctg atgttggtct aaatgctgta
     2881 cctccatcgt cagaattttt atctcggata actattgtcc ctgttttatc atcccaatac
     2941 gcagttctac cgcgaggtaa ctctttcata tttgttggat ggctaacaat attttcaata
     3001 tgccgagcaa aatctgctgg tgaattgata tttaaatccg taaattcttg ttgtcttatg
     3061 acatgcttgt tataagcatg cccactacta atttgttctc caattagttg cgcttgtaaa
     3121 gacggttgtg tggaagcatc tgctttgctg ttggacgtta tagatttagg cggtctattc
     3181 ccccctgcca tctccccatc tacagtctgc atatggcggg cagcaggttt tttatagccg
     3241 gtatttttaa cccaatccca tttggtcggg ttaagttcta cttttttacc gccccaaacc
     3301 gtagttgcgg cctctgctac ggcaagggca gtttgggctg ttgcagttat attcggatgg
     3361 gcatcagccc attgtttggc agagttgata ccgtctttta ccgcaaaagc actgtcttgt
     3421 aatgcggttg cagccgcaag ttgtgcttcg ggacttaaat ttcctaaatg attaatacct
     3481 tgtagagtct gctgcgcggc tgtatctgtg accgggctta ctgcactgtc tgtaattgcc
     3541 ccaatcccta ctccttgaaa acccattaaa aaaggattaa ccgcaccttg gacgatgccg
     3601 cgaacatcat ccatacggtt agcccaccaa tttttattgg ggtcgttttc ccatatcagt
     3661 tttcctgctt catcagcaca gttaggattg gcattgatgc ccacccagtt tcaggcagcc
     3721 tgaaaaggat ttttaatata cggcaaaagg tgggcaacaa gtgcccacct tacctagcta
     3781 acatttatcc caagtatcat atattttttt aagttcttta tccaaagaaa gaactttatt
     3841 ttttcaacac catgaattat ctcaaaatct ctacaatcca cgcatacgcc tgaataaatt
     3901 tctcctacaa ttgcttgaat aatatacaac tcatctttat cgagactaat tttaactgaa
     3961 ctctcttcaa aattaattaa tttcatgaat aattcctttt attttaattc gtcatcaaaa
     4021 taccgtttgc ctgtttttgg tctaaatgct gtcccaccat ctttagtctt ggatatttag
     4081 attataatcg tcaaattccc agtcaaaata ttttgccaaa tttgatatag acttatcttc
     4141 agacgttgaa ttacatattt ccaatatatc atttatcaat tttaatatgt gttcaatatt
     4201 aaaacggttt gtaacaattg agttttttaa tgacatcggt ctatgtatat ttgaaagtaa
     4261 ccattcagtg ctatatatat aaacataaaa ataatcagca ccattacttt catcaaaacc
     4321 tattgataat gtcaaaagta tacaaaaatt ttctaacttg tcttgtggga aatcttccaa
     4381 atcaaagtca gaacaatcat aattttttaa ttttaatctc atataattac ctaatattta
     4441 aatgtccatt tttaatttta ttcctttttc catttgaatc aatagtataa gtttcaaaat
     4501 ttgcttgaat acctgtagtt gcaaattgtg aatttttttc tgttggtggc cgatattgtc
     4561 gagtgccatc tatacttaac catccgcctc cactagtttg tcttgcaccc tcaccaaccc
     4621 aaattttacc tagcctatct gcctcttcat aagttgcagt tcctattgga aaattttttt
     4681 cactcttatg aagagctaga ctcaatcttg gatcttgagc cgcaatgttt tttaagtttt
     4741 gcccagttaa ttgattaact aattgaggat aagttgcagc attggctttt cctccagacg
     4801 ttatagattt aggcggttta ttcccaccgg ccatttcccc atctaaagtc tgcataggtc
     4861 gggcagcagg tgttttataa tcggtatttt taacccaatc ccattt
//
LOCUS       DAWHXZ010000158         4882 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000158 DAWHXZ010000000
VERSION     DAWHXZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.158"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..165
                     /locus_tag="WIE35_001639"
     CDS             <1..165
                     /locus_tag="WIE35_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ4154529.1"
                     /translation="GMQNSGLAAALAAAHFAAAPVVAVPGALFSVWHNISGSLLATYW
                     AAKAGKHKKP"
     gene            complement(318..1442)
                     /gene="rluD"
                     /locus_tag="WIE35_001640"
     CDS             complement(318..1442)
                     /gene="rluD"
                     /locus_tag="WIE35_001640"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ4154530.1"
                     /translation="MQNTSFDNESDYSDDSDFASASGTENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVQQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPKTGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            1441..2244
                     /locus_tag="WIE35_001641"
     CDS             1441..2244
                     /locus_tag="WIE35_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ4154531.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(2302..4521)
                     /locus_tag="WIE35_001642"
     CDS             complement(2302..4521)
                     /locus_tag="WIE35_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ4154532.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVFPFAPLQQAGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQTGIVPSLNASGIRYLDYLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1237 a         1583 c         1163 g          899 t
ORIGIN      
        1 ggtatgcaaa actcgggcct ggccgccgcg cttgccgccg cacactttgc cgctgcgccg
       61 gtcgttgccg ttccgggcgc attgttcagc gtgtggcaca atatctccgg ctcgctgctg
      121 gcaacttatt gggcggccaa agccggtaaa cataaaaaac cctaaacgcc tttcatcata
      181 atgccgtctg aacggggcaa acgaaaccgg cggtttccgc ccgacccctt cagacggcct
      241 ttgtttgaaa tcggcaggga cgattgtccg gctgctccga ttcggctgcc tgcgttcgcc
      301 ccgcctttca agccgcctca ctcccttaca taaaccactt cgacatcgta gtcgtcttcg
      361 ttccaatcgt catcgtcgtc cgcgccgaat ttgtcctgcc attcctcttc attgctcaaa
      421 gacgaatcca aaccggcttc aagacggagg acagacaaca aatgatatat gtcgtccgga
      481 atcggtgctt caaacgaaac ggtttcgccg gttttcggat gagtgaaact taagcggtag
      541 gcgtgcaatg cctgacgcgc acccaaactt ttaacggctt ctttcaccgt gtcgccgcac
      601 ggatggcgcg ggttgccgta aaccgggtcg gcggcaagcg gatggttggc ctcacgcata
      661 tggacgcgga tttggtgcgt cctgcctgtt tcgagcgagc attcgatata gctgtgcgcc
      721 agatagcgtt ccaacacttt gacgtgggta acggctggtt tgccgccgaa tttgacgact
      781 gccattttca ggcggttgtg cggatcgcgt ccgatttggg tttcgatttt accgtcaaag
      841 ggaacgatgc cgttggcgac ggcgcggtaa atgcgtttga ccgtgcgctc ttgaagctgt
      901 tgcacgaggg aattttgcgc cggcagggtt ttggcaacca ccatcagccc gctggtttct
      961 ttgtccaaac ggtgtacgat gcctgcacga ggtacttggc tcaattcggg acagtgcgcc
     1021 aagaggccgt tgagcagcgt tcccgtccag ttgccggccg ccggatgcac caccagtccg
     1081 gccggtttgt tgacgacgat gacggtatcg tcttcgtaaa caatatccaa agccataggc
     1141 tctggaacaa acgccagatt ttcctcactc ggacggacgg ttacacaaat ttgctcgccg
     1201 cctatcattt tgtctttggg ttgcgaaggt ttatcgttta caataaccgc gccttcttta
     1261 atccatgatg tcaggcggct gcgcgagtag tcgggcagaa gtttcgccaa caccgcatcc
     1321 aaccgcccgc cggcaagctc gagcggaacg gtcagaccga tacggttttc agttccggaa
     1381 gctgacgcaa agtctgaatc gtcgctataa tcggattcat tatcaaagga agtattctgc
     1441 atgaaaaaaa ttcttttaac ggtttcatta ggtttggcac tgagtgcctg tgccactcaa
     1501 ggtacggtcg ataaagatgc ccagattacc caagattgga gtgtggagaa gctctatgcc
     1561 gaagcccagg acgaattgaa cagcagcaat tatacgcggg ctgtcaagtt atacgaaatc
     1621 ttggaatcgc gcttccccac cagccgccat gcccggcagt cgcaactgga taccgcatac
     1681 gcctattata aagacgatga aaaagacaaa gctctggcgg caatcgaacg cttccgccgc
     1741 cttcacccgc aacacccgaa tatggattac gcgctgtatc tgcgcggctt ggtgctgttc
     1801 aacgaagacc agtccttctt gaacaaactg gcctcgcaag actggtccga ccgcgacccg
     1861 aaagccaacc gcgaagcgta ccaggcgttt gcggaactcg tccaacgctt ccccaacagc
     1921 aaatacgccg ccgatgcgac cgcacgcatg gtcaaactgg tcgatgcact gggcggcaat
     1981 gaaatgtcgg tggcgcgcta ctacatgaaa cgcggtgcat acatcgccgc cgccaaccgc
     2041 gcccaaaaaa tcatcggcag ctaccagaat acgcgttatg tcgaagaatc gctcgccata
     2101 ttggaacttg cctaccaaaa actcggcaaa ccacagcttg ccgccgatac gcgccgcgtg
     2161 ttggaaacca acttcccgaa aagcccgttt ttgacgcacg cttggcagcc cgacgatatg
     2221 ccctggtggc gttactggca ttaagcccat cttcgtaatg ccgtctgaag ccgtttcaga
     2281 cggcatttta tgtttcaaac ctcattcaaa cggttttttc tgccaataga atttataccc
     2341 tctcaaacgt tgagccttca cgccgccgcg ccccaagccg aattgcagcg cgcctgacaa
     2401 atcggtacgc agcagtttga tgccgtgtgc gcggacacgg ttctgcaccg cttcggtcgg
     2461 atgtttgtag gcgttggcat aaccgcttga agcaacagca tattcgggcg agacggcatt
     2521 gaggaacacg cccgacgagg acgtattgct gccgtgatgt cccaacacca aaacctggct
     2581 gtacaggttg cctccatact tgccgaccag gctttcctca cccttcgtat ccaaatcgcc
     2641 cgttaccagc agtgccgcac cgcccgccac aacacgcaaa acacaacttt tcccattatc
     2701 atcgatgttt ttgcgttcag acggcatcaa aaactcgaaa tccacgccgt cccattgcca
     2761 acgctgttcc gcacaatgcc gcgccccctc ataaaattcc ggctgtccgg cataaatccc
     2821 gccgttcggt atcttgccga ctgcctgaaa accgccgtcg tggtcgctgt cgtgatgcga
     2881 caaaaccaaa taatccaaat acctgattcc cgacgcattc aggctcggca caatccccgt
     2941 ctgcgccgcc gctgccgttc cggtgtcaaa caaaagatga tgatttgccg tctgaaccga
     3001 caccgacaaa ccctgccccg catcccaaac cgtaaccgca acctcatttt ccggcacgcc
     3061 gggtgaacgg taaaacacaa accctgccaa cagcaacacc gcccacggac gcaaacccaa
     3121 gccgcgcggc agcagcaaca gcaaagcggc acacaccgcc aacaccaaca gcggcaaagg
     3181 tgcggaggca acggcaaact cgggcgacac atcggcaagc cacaccaaaa accgcaaagt
     3241 gtattccgcc aaaaacgccc ccgcctgctg aagcggcgca aacggaaaaa ccgaacccag
     3301 caacgccagc ggcgtcaata cccaagaaaa ccacggaatt gccaccgcat tgaccaaagg
     3361 gctgattaaa ggcagcgaag caaacagata accgagcaaa accagcgaca acaccgaagc
     3421 cgcccactgc ccgcgcaaag cagtttgccg ttttccctcg tgcaaacgcc ccgagcacgc
     3481 ccatatcagg gccgccacca aaccgaaaga cagccaagtc cccacaccca agaccgccga
     3541 cgggtcgaac agcagcaccg ccgccaacgc ctgccaccac gtcgcccacg ccgacaatct
     3601 tcccctgcgc caagcccacg caaacgccgc caacatcaaa acgctgcgct gcgtcggcac
     3661 ggaaaaaccg gcaagcagcg cgtaaaacag cgcgcctgca caccccgccg ccaaaaccca
     3721 cgcgcgcggc cgggcaggaa ggcgcgggga acaggcaagc agccgcttcg ccagccacgc
     3781 aaacattacc gccaccatcg taacgtgcaa acccgaaata ctgaccaaat gcgtcagccc
     3841 caacggccgg aacgcctgcc acaattcggg gcgcaatgcc gactgttcgc ccacgctcaa
     3901 cgcgcgcatc agcccgatgc cgtctgaaag cccgccgtcc gcatccgcct gctgccaatt
     3961 acggctgatg cggctgcgcc aaaccgcaat cccccaaccg cttccgccat gcagcaaaac
     4021 cctgtccgca ccgaccgtcc ccgcaccgcc tattccgttg gataatgccc acgcctcacg
     4081 gttcaaaccg cgcaggttca actccccgac gacagggtgc acacgtgccg ttatccgcca
     4141 tctgctcccg accgcccatt cgcgccgttt gtagtccgac agcaacaaat caaacgtccg
     4201 accaccgctg tccacagcct ttgccgcaaa ctgcacacgc cgcccgtccg acctcggcat
     4261 atccgccact tccaccgtca acggcacacc tgaaaccgcc tccgcccgcc attgcgaaga
     4321 cagtgccgct tccgttctga atacgccgta agccgcgccc gccaacacgc acagcatcag
     4381 accggcaaac gcaaaacgcc ttgcaaacac agcaaaaacc gcaaaagccg ccagccaaaa
     4441 cagccaatgc ggcacgaccg gcagcgcaaa cgaagccgcc gcgccgacaa cccaacacgg
     4501 caaaaaccaa cgacggaaca cgtctcccca ccccagatat ttcaaaaata aacggataat
     4561 aaagcaaatc cgaaaaaccg tcataacaag atttgaaaat gatatagtgg tttaaattta
     4621 aatcaggaca aggcgacgaa gccgcagaca gtacaagcag tacgacaagg cgaggcaacg
     4681 ctgtactggt ttttgttaaa ccactataag aatgcaaacg aaaagcctca gtattatccg
     4741 agacaacggc aaactttcca tcgtcattcc cacgaaccca catcccgtta ttaccacgaa
     4801 agcgggaatc taggactcag ggttaagaaa acctacatcc cgtcattccc tcaaaaacag
     4861 aaaaccaaaa tcagaaacct aa
//
LOCUS       DAWHXZ010000159         4859 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000159 DAWHXZ010000000
VERSION     DAWHXZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.159"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..984)
                     /gene="serS"
                     /locus_tag="WIE35_001643"
     CDS             complement(<1..984)
                     /gene="serS"
                     /locus_tag="WIE35_001643"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ4154533.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITL"
     gene            1150..1674
                     /locus_tag="WIE35_001644"
     CDS             1150..1674
                     /locus_tag="WIE35_001644"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ4154534.1"
                     /translation="MLDREGYRPNVGIILTNNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            1741..3726
                     /gene="parE"
                     /locus_tag="WIE35_001645"
     CDS             1741..3726
                     /gene="parE"
                     /locus_tag="WIE35_001645"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ4154535.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVSNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDSILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTVELDI"
     gene            complement(3800..4204)
                     /locus_tag="WIE35_001646"
     CDS             complement(3800..4204)
                     /locus_tag="WIE35_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ4154536.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAEAAGSSAAE"
     gene            complement(4296..>4859)
                     /gene="aroC"
                     /locus_tag="WIE35_001647"
     CDS             complement(4296..>4859)
                     /gene="aroC"
                     /locus_tag="WIE35_001647"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ4154537.1"
                     /translation="AANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGLGEPVF
                     DRLDAEIAYAMMGINAVKGVEIGAGFGSVVQRGSEHGDELTPQGFLSNHSGGILGGIS
                     TGQDIHVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEAMLALV
                     LIDHALRHRAQNADVQVNTPDITLSNK"
BASE COUNT         1075 a         1355 c         1344 g         1085 t
ORIGIN      
        1 cagggtaatc acgcggtagg gcagttccaa agccttcagg atgttttcgg catggccgac
       61 catttcttcc agcgtttcgt atgatttttc gggatgaacg atttgaacca tttccacttt
      121 gtcgaactgg tgctggcgaa tcagaccgcg cgtgtctttg ccgtacgaac ccgcctcgct
      181 gcggaaacag ggcgaatgcg cggtcagctt cagcggcagt tgttcggacg ggataatgct
      241 gccggcaacg gtattggtca gggtaacttc agctgtcgga atcagatatt gcgtggtttt
      301 gctctcgtcg ccgccacggg taacgtggaa caaatcttct gcaaacttcg gcagctgacc
      361 cgtgccttgc aaggtggtgt cgtccacgat atagggtgtg taatgctcgg tgtagccgtg
      421 tttcagcgtg tgcgtatcga gcatgaattg cgccagcgca cggtgcaggc gggcgatttg
      481 tccgcgcatc acggtaaatc gtgcgccgga aagtttcgcg ccaccttcga aatccaaacc
      541 caaaggttcg cccaaatcga catggtcttt gatttcaaag tcaaattcgc gcggggtgcc
      601 gactttgcgg acttcgacgt tttcggtttc gtctttaccg gcaggtacgc tttcgtgcgg
      661 caggttaggt atgctcaaca accatgcgtc caattctttt tgaacggcat ccaaatcggc
      721 ggcagcctgt tccaaatcgg ttttgatttg ggcgacttga tccatcgccg cctgcgcttc
      781 ttcgtgtttg ccctgacctt tcagtgcgcc gatttgtttg gaaatgctgt tgcgcgaggc
      841 ttgtagttct tcagttttca cctgaacgga cttgcgtcgt tcttccagtg cgtcaaaacg
      901 tgcggtatca aagtcataac cgcgccgtgc aagccgttcg gcaacggcgg cggtgttgct
      961 gcggagcaat tggatatcta acatggtctg attcccgaat taatttgaaa cacattattg
     1021 taaaccaaat ttaagatatt ttcccgaatc cgtccgtgtt tcgacatgct tgaaccatac
     1081 cgtctgattt gtcaaaaccg ttcgatttgt gggaaaatcc caagattgaa tttaacggag
     1141 ggcgacaccg tgttggacag agaaggctat cgccccaatg tcggtatcat cttaaccaac
     1201 aaccgcgacg aagtcttttg gggtaagcgc gtgcgcgaac attcttggca gtttccgcaa
     1261 ggcggcatca agccgggcga aagccccgaa accgccatgt accgcgagtt gtacgaagaa
     1321 gtcgggcttt tgccgcaaca cgtcaaaatt atcggccgca cgcgcgactg gctgcgttat
     1381 gacgtgccga acaactgggt acgccgcgaa tggcgcggat cctatcgcgg gcagaagcag
     1441 atttggtatc tcctgcgcct gaccggccga gattgcgatg tcaacctgcg cgccacccgc
     1501 caccccgaat ttgacggctg gcgttggcat caatattggg cgcccgttga cgaagtgatt
     1561 gatttcaaac gcgacgttta tttgggggcg ttgaaagaac tctcttcccg cttcctgcgc
     1621 ggtatggaaa gttatgaaga ctttgccgcc cgccaacctt ccggcaaccg gtaaaacaac
     1681 ggaacgcaac gctttcaggc cgtctgaaag cgttgttttt cttactttca gattattcct
     1741 atggctaaaa acaaccaata cagcgaatcc agcatcaccg tcctcaaagg tttggaaccg
     1801 gtcaaagaac gtcccggtat gtacacccgc accgacagcc cgacccatat ctgtcaggaa
     1861 gtgattgata acgcggcgga cgaggcgttg ggcggtttcg ctacggcaat cgacgtgcaa
     1921 attcatgaag acggttcgct ttccgtacgc gacaacggcc gcggtattcc cgtcggtctg
     1981 caccctgaag aaggcgtacc cgtagtcgaa ctcgtgttca cccgtctgca cgcaggcggc
     2041 aagttcaaca aaaaagacgg cggcagcgcg tatgcctttt caggcggcct gcacggcgtg
     2101 ggcgtatccg tgaccaacgc cctttccacc cgccttgagg taacggtcaa acgcgaaggc
     2161 aaaatccacc gtatcgtgtt tgccggcggc gacgtggtcg aaccgttggc acaagtgggc
     2221 aaatgcgccg tcaaagacag cggcaccgaa gtgcgcgtgt ggccggacgg aaaatatttt
     2281 gaaagcccga attacagcat tcccgaactc gaacgcctgc tgcgtgccaa agccgtgctg
     2341 ctgccgggtg tgcgcgtttc cctgacccgt ccggtcaaag gcgaagacga agcgcacacc
     2401 caaacctggc attaccccga cggcctgaaa agctatctga ccgacctgat tgccgacgcg
     2461 caggaagccg taccgctgtt ctcttgcgaa aactacattt cagacgaaca caacggcgat
     2521 ttcagcatcg gcgaaggcgc cgcctttgcc ctgacttggc tggaagaagg ctcgtgtgcc
     2581 aacgaaagct atgtcaacct catccccacc ccgctgggcg gcacgcacga agcgggcttg
     2641 aaacaagccg tgttcaacgc cgtcagcaac ttcatcaacc tgcacaacct cttaccgcgc
     2701 ggcgtgaaag tgcaaagcga cgacgtgttc ggcaaaactg cctttgtcct ctctgcccgt
     2761 gtcctcgacc cgcagttcca aggtcagacc aaagacaaac tgaccaaccg cgacgcgctg
     2821 aaactcgttg ctaccgtatc gggcgaccct ttggaactgt ggctgaacca aaacgtggac
     2881 ttcggcaaaa aaatcgccga actcgccatc cgtcaggcac aggcgcggat gcgttcggtt
     2941 aaaaagattg aaaagaaaaa aggcagcggc gtcgccgtcc tgcccggtaa gctgaccgac
     3001 tgcgaaagcg aagacatccg cgaaaacgaa ctcttcctcg tcgaaggcga ttccgccggc
     3061 ggctcagcca aactcgcccg cgacaaagcc acccaagcca tcctgcccct gcgcggcaaa
     3121 gtgctcaaca gctttgaagt ccaccccgac caactcttcg gcaacgccga aatccacgac
     3181 atttccgtcg ccatcggcgt cgatccgcac ggtattaacg accatcccga tttaagcggc
     3241 ctgcgctacg gcaaaatcgc catcctgtcc gatgccgacg tggacggctc gcatattcaa
     3301 gttttgctgc tgaccttatt ctaccgccac ttcccgaaac tggttgccga cggacacatc
     3361 tacgtcgccc agccgccgct gttccgcgtc gatgtcaacg cacaaggcaa aagcaaacct
     3421 gcccgcaaat tctacgccct cgaccaaaac gaactcgaca gcattttgga gcggctgcaa
     3481 aaagaaggcg tcaaagaaac cgcctattcc atcagccgtt tcaagggctt gggcgaaatg
     3541 aaccccgacc agctcaaaga caccaccatg caccctgaca cccgccgcct gttgcaggta
     3601 caaatcccag aaggtgcaga tgacgagacg cgcgacatct tcgtcaaact gatgggcaaa
     3661 ggcgaagccg ccgcccgccg cgcttggatg gaacgcgaag gcgatacggt ggaattggat
     3721 atttgaaccg tgattgccgc atacgaaaaa atgccgtctg aaaccaaatc ggttttcaga
     3781 cggcatttcg tttggggatt tattctgctg cggaagatcc ggctgcttcg gcggcagctt
     3841 gggcggactg ctgttgcaaa gcctgctgca cgaaaggatg gttttgcgcg atcgcctgtt
     3901 cttcgggggt aacttcgcct ttgaaacggt tgttcaaatc gaaacgtttg ccgccgcgcg
     3961 ccaaggcttt cagataacgc ggacggcggc agtggttggc gaggacgcgt gcaatcagtg
     4021 cggaatcgta ttgcggcaaa gcggcaatca aatcctgatc aatgccgagc gccaacggtt
     4081 tgaagcgttt gaatacatcg tatttgccgt agatgtggtc ggcgatcatt tcggtctgtt
     4141 tctttttgct catagtttgg acggcggatt tcagtgccgc gcccaaagcg gtttcttgtg
     4201 tcatttcggt tgtattcctc taaacaaacc cgtgcgtcaa agcggcacgg caatgctcgg
     4261 tattctatta taaagtctgt gttttggcta aatttttatt tgtttgaaag ggtaatgtcg
     4321 ggcgtattaa cctgaacatc cgcattttgc gcgcgatggc gcagggcgtg gtcgattaaa
     4381 accaatgcca gcatagcttc tgcaatcggc gcggcgcgca agccgacgca ggggtcgtgc
     4441 ctgccgtgcg tggcgagttc gatggggttg ccgttgatgt cgatactgcg gcgcggggtg
     4501 gcgatggagc tggtgggttt gatggcgata ttgacgtgga tgtcttgtcc ggtgctgatg
     4561 ccgccgagga tgccgcctga gtggttggac aggaagcctt gcggggtcag ttcgtcgccg
     4621 tgttcgctgc cgcgttggac aacgctgcca aaacctgcgc cgatttccac gcctttgacg
     4681 gcgttgatgc ccatcatcgc gtaggcgatt tcggcatcga ggcggtcaaa aacaggttcg
     4741 cccaagccga cagggacgtt ggcggcttcg atatgcagct tcgcgccgac ggaatccaag
     4801 gatttgcgca cgctgtccat atagttttcc agctcggcaa tttggctatg gttggcggc
//
LOCUS       DAWHXZ010000160         4715 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000160 DAWHXZ010000000
VERSION     DAWHXZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.160"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            49..1557
                     /gene="nadB"
                     /locus_tag="WIE35_001648"
     CDS             49..1557
                     /gene="nadB"
                     /locus_tag="WIE35_001648"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ4154538.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKLAIEWLLAQGVAFDQ
                     NNNGLHLTREGGHTCRRIAHVADYTGEAVMQSLTVQIRCRPNIRVCEQQMALDIQTES
                     GAACGLTVLDCRTQATYSIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARSSENGRTFLISEAVRGEGGILTNQAGERFMPYYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYSLGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQAKTSPRPSESLSSEAGIFSDGIQSAFSRPVLQAFNQRHLGILRNDTGLRC
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(1735..2400)
                     /gene="pgmB"
                     /locus_tag="WIE35_001649"
     CDS             complement(1735..2400)
                     /gene="pgmB"
                     /locus_tag="WIE35_001649"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ4154539.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYLEMIQAVKPEDVYLGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSDTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(2413..4671)
                     /locus_tag="WIE35_001650"
     CDS             complement(2413..4671)
                     /locus_tag="WIE35_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ4154540.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALSGSQTDWQVSNSFEVEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVVFETLLDAHKAGWLHRWEIADVVIEGNDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRSGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVSAAELEKWADISANMYRPYDEELGVFVQHDGFLDKGIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLDGSHAAALEK"
BASE COUNT          956 a         1341 c         1350 g         1068 t
ORIGIN      
        1 ttaatccact ataccgtttc agacgatctc tcaaacaagg acaacaccat gcaaaccgat
       61 tgcgacgtat tgattgccgg aaacgggctg gcggcactga cgctcgccct gtcgctgcct
      121 gaatcgttcc gcatcgtcat tttgtgcaaa aaccggctgg acgacactgc cagccgtcat
      181 gcgcaaggcg ggattgcggc ggcgtggtcg ggagaggacg acatcgaaaa acacgttgcc
      241 gataccttgg aggcgggcgc gggtttgtgc gacgaagccg ccgtccgcac catcctgtcg
      301 cagggcaaac tggcaatcga atggctgctg gcgcagggcg tggcgttcga ccaaaataat
      361 aacggcctgc acctgacgcg cgaaggcggg cacacctgcc gccgaatcgc ccacgtcgcc
      421 gactacacgg gcgaagccgt catgcagagc ctgactgtcc aaatacgctg ccgcccgaac
      481 atccgcgttt gcgagcagca gatggcgttg gacatccaaa ccgaatcagg tgcggcgtgc
      541 ggactgaccg tcctcgactg ccgaacgcaa gcaacctaca gcatccgcgc ccgccatacc
      601 gtactcgcag gcggcggctt gggacagatt tacgccgcca ccaccacgcc gcccgaatgc
      661 acgggcgacg ccatcgccat ggcgatacgc gcaggctgcg cagtcgaaaa cctcgaattt
      721 atccaattcc accccacagg cttggcaaga tcgtctgaaa acggccgcac cttcctgatt
      781 tccgaagccg tgcgcggcga aggcggcatc ctgaccaacc aagcgggcga acggtttatg
      841 ccgtattacg accgccgcgc cgaactcgcg ccgcgcgaca tcgttgcccg cgccatcgcc
      901 gccgaaatcg ccaaacaaac gcaagacttc gtctcactcg atatcagcca tcaacccgca
      961 gcgttcgtcc gccggcattt cccgtccatc catcggcact gtttatccca atgcggcctg
     1021 gatatcacgc gccaagccat ccccgtccgc cccgtgcaac actacacctg cggcggcatc
     1081 caaaccgacc cctgcggcag aacttccctg ccgcagctct actccttagg cgaaaccgcc
     1141 tgcaccggct tgcacggagc caaccgcctt gccagcaact ccctgctcga atgtgtcgtt
     1201 accgccagac ttgccgccca aaccatcgca gacggacaag cattccaagc caaaacgtct
     1261 ccaaggccgt ctgaaagcct ctcctccgaa gcaggcatct tttcagacgg catccaaagc
     1321 gcattcagcc gccccgtcct gcaagcgttc aaccaacgcc atctgggcat cctccgcaac
     1381 gataccggtc tgcgctgcgc catcgcccag ctgcggcttt ggaaacaaaa ccaaaccgaa
     1441 ccgcacaccg cgtccgaata cgaaaaccgc aacctgctcg aatgcagcct cgccgtcgcc
     1501 caagccgcat acaggcggag acagaacatc ggcgcgcatt ttaatagtga ttgttaaggt
     1561 tggctggtta gaaaaaaaat cgttcaaata aatttgaata gcatggttaa gaaattggca
     1621 tttagttttt agggttgcgt aagttgggtt atcaatctgt tctaaaagca gaaaggccgt
     1681 ctgaaacact gttcagacgg ccttttactt ccttgacact ttatctgacg cgttttacct
     1741 gcccgactgt gcccacacgt tttgcaggta ggcgtaggtc agctcggcgg tatcggacac
     1801 cagcgcgatg tcgctgccca agtcttcggc tttgcccacg ccgataggca aggcgccggc
     1861 ggctttgatg gcggcgacac ctgcggcagc gtcttcaatg ccgatgcagc ggcggatgtc
     1921 cgcgcccacg ccctcggcgg cggcgaggaa gatgtcgggg gcgggtttgg aatgcgcgac
     1981 ggcggcaggg tcggcgacgg tgtcgaagaa gtgggtcagc cccatgcgtt cgagcaggaa
     2041 ggggccgttt ttactggcgg acgcaagggc gatttttttg ccgtttgctt ttaatgcttc
     2101 cagcagcggc aaaatgccaa gatacacgtc ttcgggtttg actgcctgta tcatctccaa
     2161 gtagttgtcg tttttgcggc gggtcagttc ggcgaactcg gcttcgccga cggttttacc
     2221 gccgtgcgcg aggatgcgtt tgagcgaatc gtcgcgcgac acgcctttga gctgctcgtt
     2281 aaacttgcgg tcgatgccga tgcccagttc ttcggcgagt tttttccacg cgcggtagtg
     2341 gtattcggcg gtgtcggtga tgacaccgtc gagatcgaag aggactgcgg taaacgtcat
     2401 tttgcaccct ccttattttt ccaacgcggc ggcgtggctg ccgtcgagcg tgatgtcttt
     2461 gccatacacc tgcaaatcga gcggctcgcc tttgagcagg gtgaagacga cgttttcttt
     2521 gccgacggcg actttaatca gacggcctcg gtagttgata tggaaggcgt agcctgtcca
     2581 tgcgcccggc aggaacggtg cgaagctgag tttaccgccc caagttttca tttgggcgaa
     2641 accttggacg atggcgagcc acgagccggt catagaagta atgtgcaggc cgtcttcggt
     2701 gtcgttgttg tagttgtcca agtccaggcg ggcggtgcgc tggtacattt ccacggcttt
     2761 ttcttctttg cccagttcgg cggcgagaat agagtggata caaggcgaca acgagctttc
     2821 atgcacggtc atcggttcgt agaagtcgaa gttgcggcgt ttttcgtcga tattgaaacg
     2881 gtcgccgaag aaatagatgc cttgcaatac gtccgcctgt ttgataaagg gcgaacgcag
     2941 gattttgtcc cacgaccatt tctggttgag cggcaaatcg tcgggcgaaa gcgcggacac
     3001 ggggcggatg cctttgtcga ggaagccgtc gtgttgcacg aatacgccga gttcttcgtc
     3061 atacggacgg tacatattcg cgctgatgtc cgcccatttt tccaactcgg cggcgctcac
     3121 gttcaaatcc ggacgcgggt atttcgccag ggcttcgcgg gtgtagtcca atacccatgc
     3181 ggcgagggtg ttggtgtacc agttgttgtt gatgttgttt tcgtattcgt tcgggccggt
     3241 tacgccgtga atcatgtatt tgccgctgcg tttggagaag tggacgcggt ccgcccagaa
     3301 gcgggacact tcgaccaaga cttccaagcc ttctttggca agatagccct cgtcgccggt
     3361 gtagttggtg tagttgtaga tggcgtaagg aatcgcgccg ttgcggtgga tttcctcgaa
     3421 ggtgatttcc cattcgttgt ggcactcgat gcccgtaaac gttaccatcg gatagagtgc
     3481 gcccgccaag ccctgttcgc gcgcgttgtg ttgcgcctgt ggcagttggt tgcggcggta
     3541 ttgcagcagg ttgcgggtaa cttcgggttc cgccagtgcg aggtagagcg gcacggcgta
     3601 ggcttcggtg tcccaatagg tcgcgccgcc gtatttttct ccggtaaagc ctttcgggcc
     3661 gatgttcaga cgcgcgtctt cgccgtagta ggtggagaac agttggaaca gattgaaacg
     3721 gatgccttgc tgcgcttcgt cgttgccttc gatgaccacg tcggcgattt cccaacggtg
     3781 cagccagcct gctttgtgcg cgtccagcaa ggtttcaaac acaacgcctg cgactttttc
     3841 cgacaaggcg cggcctgcgg ctttcactgc ttccaagccc tgataatcgc ggctggtggt
     3901 aacaataacc cgtttttcaa aggttttggg cgtgccgccg acttcgacct cgaaagaatt
     3961 ggagacttgc cagtcggttt ggctgccgct gagggctttg aagctgccgg cgaaagtttg
     4021 ctcggcgttg acgataaatt gttccacgcc gaaagggttg gcaacggttt gggcggcaat
     4081 gtaggagaga ctgtctgaaa cgcctttgtc caatacctgc cagaattttt cttcgtagtt
     4141 ggagtcttcg tttttcacgt cggcatcgat gatggaatcg atgcggactt ggtgggtttt
     4201 gccgtcaacg gatacggctt cccagcggat aaccgccagc tctttttgcg cgacggacag
     4261 gaatttgcac acattgaaac gcacaccgaa tacggtgaac gagcggcgca acacgccgtg
     4321 ctgcatatcg agttcgacgg agaagccggc aacatcgttt ttcgccaagt ccacctcttg
     4381 tccgtcgaca aagattttga ctttgctgaa attgagcgcg ttgatggctt taccgaaata
     4441 tttggggtag ccgtttttcc accagccgac gcgggttttg tcgggaaacc acacaccggc
     4501 gatgtaggta cccaagtggc tgtcggcgga ataggtttcc tcaaagctgc cgcgcatacc
     4561 catatagccg ttgcccaaac tggtcaggct ctcttgcagc cgtttgtgtt ctttttccag
     4621 ctttgccgaa cgcagcgtcc aagggctgat ttccataatt cttgtgtaca tttatgaagc
     4681 tcctgtttgg attgatttga gggaatggtg aaatc
//
LOCUS       DAWHXZ010000161         4705 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000161 DAWHXZ010000000
VERSION     DAWHXZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.161"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            47..451
                     /locus_tag="WIE35_001651"
     CDS             47..451
                     /locus_tag="WIE35_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ4154541.1"
                     /translation="MTAQPCPICTAQDEDILLQTPNLRVIAIHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
     gene            491..1675
                     /locus_tag="WIE35_001652"
     CDS             491..1675
                     /locus_tag="WIE35_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ4154542.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNVGFTVTNQQAGSRRF
                     QRFAGTDSQRAADFQDVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLNASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(1738..2271)
                     /locus_tag="WIE35_001653"
     CDS             complement(1738..2271)
                     /locus_tag="WIE35_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ4154543.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(2450..4705)
                     /locus_tag="WIE35_001654"
     CDS             complement(2450..4705)
                     /locus_tag="WIE35_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ4154544.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSQEAREKLFNFFFWTSWSASTNRPDENFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTISAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLIFIGGAICVAI
                     QATKIVFGRDK"
BASE COUNT         1177 a         1320 c         1178 g         1030 t
ORIGIN      
        1 agaaattatc ctaccgtttt ttaaacactt tcaggaataa ggaaaaatga ccgcccaacc
       61 ctgccccatc tgcacggcac aagatgaaga tattttgctg caaaccccca acctccgcgt
      121 catcgccatc cataacgaca gcggttcgcc tgcattctgc cgcgtcattt ggcgtaagca
      181 catcgccgaa atgaccgacc tttcggcagc ggaacgcggc gaattgatgg aaatggtgta
      241 caaagtcgaa gccgctatgc gccaagtgtt tcaacctgcc aagattaacc tcgccagctt
      301 gggcaatgtc gtgccgcacc tgcactggca tattatcgcc cgctttgaaa acgatgcgtc
      361 cttccccgcg ccgatttggg caaaccccgt ccggaaacac ggtatgaccc tgccgcaaga
      421 ttggacggaa cagcttaaaa agctgcttta agcccgccga tgccgtctga aaccgtatga
      481 aagggaaatt atgaccgaac cgacctcccg ccgccgtttt ctgaaaacct gcaccgccgc
      541 tgccggcgcg gggctgcttc aggcttgcgg cacatccgcc acatccgttc cgccccttcc
      601 ctcttcccat tccgttgtga aagcccgaac cgtgcctctc caaacgccac gccgtcaaag
      661 ttcggacggc aaccttctgc gcgttgtcgc ttcgtcagga tttgccgaag acaccaaccg
      721 cgtcaacaca gccttaaccc gcctttacaa tgtcggtttt accgtaacca accaacaggc
      781 gggcagccgc cgtttccaac ggtttgccgg cacggactca caacgtgccg ccgatttcca
      841 agatgtcgct tccggtcgcg ttgccacacc taaagtgctg atgggcttgc gcggcggtta
      901 tggtgcggca cgcattctgc cgcatatcga ttttgcttcg ctcggcgcaa ggatgcgcga
      961 acacggcacg ctgtttttcg gattcagcga cgtatgcgcc gtccagctgg cattgttggc
     1021 aaaaggcaat atgatgagtt ttgccggccc gatggcttat agcgattttg gcaaacccgc
     1081 ccccggtgcg tttacgatgg atgcctttat caagggtgca acccaaaacc gcctgaccgt
     1141 tgatgttcct tatatccaac gcgccgatgt cgaaaccgaa ggcacgttgt ggggcggcaa
     1201 cttaagcgtc ctcgcctcgc tcgccggtac gccttatatg cccgacatcg acggcggcat
     1261 tttgttcctc gaagatgtcg gcgaacagcc ctaccgcatc gaacgtatgc tcaatacgct
     1321 gtatctttcg ggtattttga agaaacagcg cgccatcgtg ttcggcaatt tccgtatgga
     1381 aaaaattcga gatgtctatg atccgtctta tgatttttcc gccgttgcca accatgtttc
     1441 gcgcacggcg aaaatccccg tgctgacggg cttcccgttc ggacacattg ccgacaaaat
     1501 cactttccct ctaggcgcgc acgcccgaat ccgtatgaac ggaaacagcg gttattcggt
     1561 cgcgtttgaa ggctacccga cactcaatgc gtccgccctg actttggata ccctgctccc
     1621 accgccggat ttgcccatct tccccgaaag cggtgttgcc gatatttcgg aataaacccg
     1681 caaacggaca aatgccgtct gaagccttca gacggcattt cccaagacgg cggcagatta
     1741 cagcaatgcc cgaatatcgg cttcgatttc ttcgggcgta acactaggcg caaaacgctc
     1801 gaccacttcg ccgtcgcggt tgacgaggaa tttggtaaag ttccatttga tgtcgccttc
     1861 gtcgcgcttc tctcccaaag ctgcgagctt caacacgaaa tctttaaaca gatgattgcc
     1921 tttatcttgc ggtttgacgg atttcaggta ggcatacaag ggcgcggtat ttgctccatt
     1981 gacttcgatt ttgtcgaaaa tcttaaactt cgtgccaaac ttcatcatac acacttgggc
     2041 aatttctccg ctgctttcgg gagcctgttc gcggaactgg ttgcacggaa aatccaaaat
     2101 ctccaagcct tctgcggtat attgtgcata cagcttctgc aaagcctcgt attgcggggt
     2161 cagaccgcaa cgcgttgccg tgttgacaat cagcagaacc ttgccgcgat agcctgacaa
     2221 atcaaccgca ttgccttccg catctttcat ttgaaaatcg taaataccca tttttatcct
     2281 tatctgatgt aaaccgatgc catctgaaac gtgcttcaga cggcatgaaa gcagcaattg
     2341 tatagccgat taaaataaaa aatccacatc cttttccatt cccgtcccaa tccgcaataa
     2401 aaaactgcac ccgaaaacgg gtgcagttgc tcatttcata ccgcaaaact tatttgtcgc
     2461 ggccgaatac gattttagtg gcttggatgg caacgcagat tgcaccgccg atgaagatca
     2521 agtcagcagc agtacgaacc caacgcagag tatccaagat ttccatttgc aggaactctt
     2581 cgctacgcgc ataccacagg ccgtgcgtga tagaggcgta tgcctgaatc gcgccgacag
     2641 gcagcaggct gatggcaatc ataccgacca agccgccgtt gagcagccag aaaccccaag
     2701 tcatcaattt gtcgtcaaac tgcgcgttcg gtttcaaata acgggcaacc agcaatacga
     2761 agcccaatgc caagaaaccg tacacaccga acaaggcggc gtgcgcgtga acggcagaag
     2821 tgttcaaacc ttggatatag aacagggaaa tcggcggatt gatcaggaag ccgaatacgc
     2881 cggcaccgat catattccaa aaggcgactg ccacgaagca catcagcggc caacgcaggc
     2941 gtttcgccca gtcggacagg tgttggtaag accagtgttc gtatgcttca cggcccagca
     3001 acaccagcgg cacgacttcc aaagcggaga agcaggcacc gattgccata gaggcggagg
     3061 tagagccgga gaagtacagg tggtgcagcg taccaggaac gccgcccaac ataaagatgg
     3121 cggcagcggc caaagtggag gcagtggcgg tactgcggcg gacaaagccc atattgtaga
     3181 agacaaaggc aaaggcggca gtggcaaata cttcaaagaa gccttccacc cacaggtgaa
     3241 ccacccacca acgccagtat tccataacgg caatcgggga tttttcaccg tagaacaggc
     3301 ccggcgcgta gaacacgccc acgccgacca tagaagcgac aaagattgcc agcaggtttt
     3361 tgtccacgcc tttttcttta aaggcggaaa tcgtgcaacg cagcatcagg aacagccaca
     3421 acaacagacc gaccatcagc agaagttgcc agaaacgtcc caaatcgagg tattcgtaac
     3481 cttggtgtcc gaaccagaag ttaaattccg ggggaaggat gtgcgtcaac gcgaagaagt
     3541 tgcccgcgta agaaccgccg accacgatga agagggcgat atagaggaag tttacgccgg
     3601 cacgttggaa cttgggatct ttgccgccgt tgacaatcgg cgcgaggaac aagcctgccg
     3661 tcaaaaagcc ggttgcaatc caaaagatgg cggattggat gtgccaagta cgggtcaggg
     3721 cgtaggggaa ccagtcggac atttcaaagc ccaacgcctc gtcaatgccg tagaaaccct
     3781 ggccttcgac ggtgtagtgc gcggtcagac cgcccagcaa tacctgtacc acaaacaggg
     3841 cgaccgtcag gaagacgtat ttgcccaatg ctttttgcga aggggtcagt tggattttgg
     3901 aaatcgggtc ttcagacggc acttccactt cttcgtgttt ggtcaggaag gaataacccc
     3961 acatcagcaa accgatgccc atcagcagaa gaacaacgct ggtgaatgac cacatatagt
     4021 tttcagtggt cggtacgttg ttgatcaaag gttcgtgcgg ccagttgttg gtgtaagtaa
     4081 aattctcgtc aggacggttg gtcgaagcag accaagaagt ccagaagaag aagttgaaca
     4141 gtttttcacg cgcttcttgg cttggcaatg tgttgttttt cattgcaaag tgttcgcggg
     4201 tggtttgcag cgcgggatca tcgccgtaaa caccgtgata ataaggcagg atgctttcga
     4261 tggcttttac acgcgtatcg ctgatgacca cagaaccatc ttctttcaca cggctttgat
     4321 tacggtattc gtcagccaag cgtgttttca agacagcttg ttcctcaggg gaaacttcat
     4381 cgaatttttt gccgtaagtc tgttgcgcgg tcaaatccaa ccaggcggac aactcacgat
     4441 gcagccagtc ggccgtccag tccggagcct gatatgcgcc gtgacccaga atcgaaccga
     4501 cttccatacc gccggtactc tgccatgcag actgacctgc caaaatatcg tctttcgtca
     4561 tcaagacttt gccggatgcg gaaacgacct gttcggggta aggcggggct ttcttataaa
     4621 cctcgctgcc catatagcca agaatggtaa agcataccgc cagaacggca aacagcaagt
     4681 accacagctt cttgtactgt cccat
//
LOCUS       DAWHXZ010000162         4698 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000162 DAWHXZ010000000
VERSION     DAWHXZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.162"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(531..776)
                     /locus_tag="WIE35_001655"
     CDS             complement(531..776)
                     /locus_tag="WIE35_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154545.1"
                     /translation="MLKSIELNSHVRNRLAAYLKGRGMDFQTAMQEEEGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            893..1318
                     /locus_tag="WIE35_001656"
     CDS             893..1318
                     /locus_tag="WIE35_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ4154546.1"
                     /translation="MYSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYVR
                     LSDMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLILLAYQLYCGVL
                     LRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            1330..1806
                     /locus_tag="WIE35_001657"
     CDS             1330..1806
                     /locus_tag="WIE35_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ4154547.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGENAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(2156..2644)
                     /locus_tag="WIE35_001658"
     CDS             complement(2156..2644)
                     /locus_tag="WIE35_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ4154548.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAGRTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(2712..3767)
                     /gene="aroG"
                     /locus_tag="WIE35_001659"
     CDS             complement(2712..3767)
                     /gene="aroG"
                     /locus_tag="WIE35_001659"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ4154549.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            4119..4324
                     /locus_tag="WIE35_001660"
                     /pseudo
     CDS             4119..4324
                     /locus_tag="WIE35_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697603.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4413..4691
                     /locus_tag="WIE35_001661"
     CDS             4413..4691
                     /locus_tag="WIE35_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154550.1"
                     /translation="MRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVGKQLTDSVGLE
                     FDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK"
BASE COUNT         1157 a         1202 c         1097 g         1242 t
ORIGIN      
        1 atcggggatg tccggggttt aaaggtctgg attcccgcct gcacgggaat gacggtttag
       61 aaatcacctg aaacgagaaa aagaaccgaa accagataag tcggattccc gcacgggagg
      121 gaaggacggg atttcaggtt actgtttttg attttctgtt ttgtgggaat aacgggattt
      181 tggatgatgt gcattttcgg gaaaactaaa aaccctccgc cgtcattccc gcgaaagcgg
      241 gaatctagaa ttttaatgcg gcaaaaattt atcggaaaaa accgaagttt aaagacctag
      301 attcccgcct gcgcgggaat gacggtttag aaatcacccg aaacgagaaa aagaaccgaa
      361 accgaacaag ccggattacc gcctgcgcgg gaatgacggc tcaaaagtta cgaaacgaaa
      421 aataaccgaa accagacaag tcggattccc gcgcgggcgg gaataacgat aagcgtaatt
      481 tcaatctgcc atattgtctt ttcacttgcc tgaaaacaga tttcagccgc ctatccctgc
      541 atccggcggc tgatgtagtc ggcaatcaaa tcccgctttt cccaaaaaaa tttctgcatt
      601 ttttgttcgg aatacagttt gcggaccaga gtgggcagac cgctgtggac gatggcggcg
      661 atttctttgt tgccttcttc ttcctgcatt gccgtctgaa aatccatgcc cctgcctttc
      721 agatatgctg caaggcggtt gcggacatgc gaattgagtt cgatagattt gagcattttt
      781 cctccgtttt cgataacgat tatatttaat ccgtctgtat gccttatttt atcatgtttc
      841 acgatcggtt tgcctgtaca ataccgcttt tcagtatgat gtgctggcat ttatgtattc
      901 ttggttcaag ctgtttcact tgttttttgt catttcgtgg tttgcagggc tgttttacct
      961 gccgaggatt ttcgtcaata tggcgatgat tgatgcgccg cgcggcaatc ccgagtatgt
     1021 gcgcctgtcg gatatggcgg tgcggctgta ccgttttatg tcgccgttgg gcttcggcgc
     1081 ggtcgtgttc ggcgcggcga taccgtttgc cgccggctgg tggggcagcg gctgggtaca
     1141 cgtcaaactg tgtttgggct tgatactctt ggcttaccag ttgtattgcg gcgtgctgct
     1201 gcgccgtttt caggattaca gcaatgcttt ttcacaccgc tggtaccgcg tgttcaacga
     1261 aatccccgtg ctgctgatgg ttgccgcgct gtatctggtc gtgttcaaac cgttttgacg
     1321 gagcatctta tgccgataga aatcgaacgc cgttttttga ttggaaacga caactggcgg
     1381 caatacgccg acgaaccgct gctgttgagg cagggttatc tgtctgttga aaaagaacgc
     1441 accgtccgtg tccgcattgc cggacaacgg gcgtggctga cgctgaaagg ctatatttcg
     1501 gaaatcagcc gcagtgagtt tgaatacgaa atcccgcttg ccgatgcgga aaagatgatg
     1561 gaaacgatgt gcccgtttaa aatggaaaaa tggcgttatc cggtcagatg gggcggcagc
     1621 ctgtttgaaa tcgatgtttt ccttggcgaa aacgcgcctt tggtcgtcgc ggaaatcgag
     1681 ttgcccgacg agaatgccga ttttgaccgt ccggactggc tggggcgcga aatcactgcc
     1741 gacggtatgt ttaccaatgc ttatttgagc aggcatccgt tctcaagctg gaaaaatgcc
     1801 gtctgaaacg gcttcagacg gcattctccg acaatcatcg caaatccaaa gacagcctct
     1861 atatccttat ttctgctttc tttgttccat tattcccaat aaatccccat aacctaaaat
     1921 ctcgtcattc ccgcgcaggc gggaatccgg ttctttaaac ttcggttttt tccgataaat
     1981 tgctttagct tttcgtttcc ggattcccgc ttttgcggga atgacgatgg aagattgttg
     2041 ttatttcgga taaattaccc taactttaaa atcccggatt cccatcttcg ggggaataac
     2101 aatattttct atttcagccc atcatttgcg ggggatttta tagagagttt atatttcaat
     2161 atctcaaata atgcacaaaa gcatagcgcg tgcctttgct gctgacgcgg cgttccgtcc
     2221 gccctgcctc tttccaatgc gtccggtcta tttcggggaa aaacgcatct ccttccacag
     2281 acaaatccac ttcggttatc cgcaaatcgg tcgccaatgg catcgcttgt ccgtatatct
     2341 gcgcgccgcc cataatgacg gcttcttccg cgcctgcgca caatgccaat gccgcctcca
     2401 aacttgccgc cgtttccgcg cctgccgcgc aataatccgc ctgccggctg atgacgatgt
     2461 tcctccgtcc gggcaggggt ttgacgggca gggattccca cgttttccgc cccataatga
     2521 cgggtttgcc caaggtatag gctttgaaaa atgcgaaatc ttcggggatg tgccaaggca
     2581 tagcattgcc cgccccgatg cacaggtttt ccgcacacgc cgcaattagg gttattttga
     2641 gcatatttcc tccacaaaaa tgccgtctga acgcttcaga cggcatttta tgacattccg
     2701 tgtcaaaaat ctcaaccggc gcgcgccata cgttttttgt ttgcacctgc caacaatgcc
     2761 aacagttctt cagtcgcgcc ccaaccgata cacgcatcgg taatgctttt gccgtacact
     2821 tccggcttat cctgtctgcc ttcgaccaaa tggctttcca ccatcacgcc cataatattg
     2881 ctcccgtcct gttccaattg ggcagcaata tcttgtgcca cttccatctg ccgggtgtaa
     2941 tccttgcggc tgttggcgtg gctgcaatct atcatcagct tatcggttac ccctgccgca
     3001 cgcagttgtt ccgccgcctc gctgacgtgt cccgcatcat aattcggctc ttttccgccg
     3061 cgcaaaatga catgacagtc gggattgccg ccggtatgga caatggcgga atgtccggcc
     3121 ttggttacag acaagaaatg atgcgaatgg ctcgccgcac cgattgcgtc gatggcaatc
     3181 ttcaaattgc cgtccgtacc gtttttaaag ccgacggggc aggacagccc gcttgccaat
     3241 tcgcggtgaa cttggctttc ggtcgtccgc gcaccgattg ccccccaaga aatcaagtcc
     3301 gcataatatt gcggcgtaat catatccaaa aactcggtag aggcaggcat acccatattg
     3361 ttcagcgaca acaacaggct gcgcgcctga cgcaaaccga aattgatatc gaacgtaccg
     3421 tccaaatgcg ggtcgttaat caaacctttc caacccaccg tcgtcctcgg cttctcgaaa
     3481 taaacgcgca tcacaatcaa aagctcgttt tcatactgct tgcggagttt caacaaacgc
     3541 tccgcatatt ccaacgccgc tttcggatcg tgaatcgaac acggcccgat aataaccaac
     3601 agccgcttgt ccctgccgtg aaccaaatcg gaaatttcct gacgggtgcg gtgaaccaag
     3661 cccgaagcct ctttggaaat cggcagctcg taaagatggg caatcggcgg caacaactct
     3721 ttaacttctt taatcttaat atcgtctgtg gggtaatggt gtgtcatctt gcatctttcc
     3781 tgatggtttt ttatacgcgg cttaggttac atcgtttacc ttgcacaaac aagtgcaaca
     3841 tattactaca aagctatttt atttcaaaat aactgatata tcttttttca tagattttaa
     3901 atttcatatt tttaatattt taggaatttt attccaaaga aaaagaaaca gattgataaa
     3961 aacaataccc gccattcatt cggacaaaac cgcacggaac atccgttttc ccatttccac
     4021 gacaatccgg tacagaacac cccgctgccg attccgcctg cacatccggc aaaatttcat
     4081 acacaaggaa aatgttgaaa acaaaaagaa ctcattgctt gaaaaaagat ttaatgggaa
     4141 tgcgggtatt tcccacactt tttccattta tttatcctca ttataggaag aaaaacatga
     4201 aaaaaccctg cttgcactct cacttgcagc cgtgtccgcc gttacggtag ccgatattcc
     4261 cgagtctaac ggcaaaggca aagccatcat cggcgataag gaaaaacacc gttaccaagc
     4321 atgaaaacct tgctttgaaa gtccatggcg aaatccgcca agatttgggg gacggatgat
     4381 ccctaacccc ttgggcggat gcatatgcag atttgcgcgg caaaaccaaa gtgatgacga
     4441 cccagatgtg tgcttcccgc gatgtcagca aaagcgccaa aggttggagt gtcggtatcg
     4501 gtctgaatgt aggcaaacag ttgaccgaca gcgtcggtct cgagtttgat ccatactacc
     4561 gtcacaaaac aatctgcaaa ccccgtgaga ttgttttgga cggcgacaaa accaaaatgg
     4621 gccgctccaa atccaacgag tacggcttcc gcgtagccgc aacgttctat agtcaattaa
     4681 aatcaaaata ggacagta
//
LOCUS       DAWHXZ010000163         4652 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000163 DAWHXZ010000000
VERSION     DAWHXZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.163"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..2848
                     /locus_tag="WIE35_001662"
     CDS             <1..2848
                     /locus_tag="WIE35_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ4154551.1"
                     /translation="TAVFDVHTLDSDGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLS
                     GLPGVQKEGTDGIITGVAFVLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDS
                     VRLAGLEHLDWRYVRAVGYATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARA
                     RDGEGFIAVSPEARKTFWLDRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERIN
                     IELSIQNKLKLCAALEQYLSGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWEW
                     LLANLDAPLADYKVRYGSAVHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFS
                     GKTDTKIIQGLGKIHAKTVRSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVE
                     RIMKIARSLGGVISGEHGIGITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSD
                     LRNAYTPSFELLGAESLIMEKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPR
                     NKILGVGLLTEAFLYEEQTRRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVT
                     VAVRNYLADSGHKRFAPAAAMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKL
                     LPIGTKKQKAEPKATVGKAPIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIR
                     NPAAPEDAEAVFYFPGCGSERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGG
                     NKAKAEEMSTNNRVAFHRMANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIID
                     IHEYLLEKGVKLDGVKGQQYLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESG
                     MFAVKRPDIATQVKFRKQEEIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPA
                     DYIVIEMAKHILGENWLDEFVKKANNGGVEKVLL"
     gene            2925..3926
                     /locus_tag="WIE35_001663"
     CDS             2925..3926
                     /locus_tag="WIE35_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ4154552.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(3988..4170)
                     /locus_tag="WIE35_001664"
     CDS             complement(3988..4170)
                     /locus_tag="WIE35_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154553.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            4357..>4652
                     /locus_tag="WIE35_001665"
     CDS             4357..>4652
                     /locus_tag="WIE35_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ4154554.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIP"
BASE COUNT         1231 a         1410 c         1092 g          919 t
ORIGIN      
        1 aaccgccgtg ttcgacgttc acacgctgga ttcagacggc ctcaatatcg ttaaaaccga
       61 acgtttggaa attcccggcc acaaatttcg caaagtcggt ttgggcaaag acgttaccga
      121 caaattcttg agcggcctgc ccggcgtaca gaaagaaggc acggacggca tcatcaccgg
      181 cgttgctttc gtgttgcaca aaatgccgaa atacacgcgc actgtgtgta tggagttttt
      241 cggcacggtc gccaccgcta cgccttctat cgtcgaaatc cgcgatttcc tgctcgccca
      301 cgacagcgtt cggctggcag gtttggaaca tttggactgg cgttatgtcc gcgccgtcgg
      361 ctacgccacc aaagcggcag gcaaaggccg gccgaaaatg gtcttactgg cggacgtggt
      421 ttcagacgac gaagccgccg tagaggcagc cgccgagcac atctgtgaac ttgcccgcgc
      481 ccgcgacggc gaaggcttta tcgccgtatc gcccgaagcg cgcaaaacct tctggctcga
      541 ccgcagccgc accgccgcca tcgccaaaca caccaacgcc tttaaaatta acgaagacgt
      601 ggtcatccca ctcgaaaggc tcggtgagta ttcagacggc atcgaacgca ttaacattga
      661 gctttccatc caaaacaaac tcaaactctg tgccgccttg gaacaatatc tgtccggcaa
      721 actccccatc gacaaaatgg gaaccgacct gccgaccgcc gaacttttgg gcgaacgcgg
      781 caaacacgcc cttgcccacg tttccgccgt caaagcgcgt tgggaatggc tgcttgctaa
      841 cttagacgcg ccgcttgccg actataaagt acgctatggc tcagccgtcc acgccgcgcc
      901 cgaagccaaa gacaatgaaa gctgctttac cgcattccgc gatttccgcc tgcgcgtgtc
      961 tgtcaaagcg gacgtaatga agccgctttc tgaaatcttc agcggcaaaa ccgacaccaa
     1021 aattatccaa ggcttgggaa aaatccacgc aaaaaccgta cgcagccgcg tctttgtcgc
     1081 cctgcatatg cacgccggcg acggtaacgt tcacaccaat attccggtta actcagacga
     1141 tgccgaaatg cttcagacgg cataccgctc agtcgaacgc attatgaaaa tcgcccgttc
     1201 gctcggaggc gtgatttcgg gcgaacacgg catcggcatc accaagctcg aatttttaag
     1261 cgacgaagaa atgcagccgt tttgggacta caaaaaccaa gtcgatccca aacacacctt
     1321 caaccgtcac aaactgatga aaggctcgga cttacgcaac gcctacacgc cgtctttcga
     1381 gcttttgggc gcggaatcgc tgattatgga aaaatcagac ctcggcacga ttgccgattc
     1441 cgtcaaagac tgcctgcgct gcggcaaatg caaacccgtc tgctccaccc acgttccgcg
     1501 tgccaacctg ctgtacagcc cccgcaacaa aatcctcggc gtgggtttgc tgaccgaggc
     1561 cttcttatac gaagaacaaa cccgtcgcgg cgtttccatc aaacactttg aagaactcat
     1621 ggacatcggc gaccactgca ccgtgtgcca ccgctgcgtc aaaccctgcc ccgtcaacat
     1681 cgacttcggc gacgtaaccg tagccgtccg caactacctt gccgattccg gccacaaacg
     1741 atttgcgcct gccgcagcta tgggtatggc gtttttgaac gccaccggcc cgaaaaccat
     1801 caaagccctt cgcgccgcca tgatacaaac cggcttcccc gcgcaaaact ttgcctacaa
     1861 aatcggcaag cttcttccga tcggcacgaa aaagcaaaaa gccgaaccca aggcaaccgt
     1921 cggcaaagcc ccgattaaag aacaggttat ccatttcatc aaccgcccac tgcccaaaag
     1981 cgtacccgct aaaacaccgc gctccttatt gggcatcgaa gacggcaaaa gcatccccat
     2041 catccgcaac cccgccgcgc ccgaagatgc cgaagccgtg ttctacttcc cgggttgcgg
     2101 ttcggaacgc ctgttcagcc aaatcggact tgccgttcaa gccatgctct ggcacgtcgg
     2161 cgtacaaacc gtcctgccgc ccggctatat gtgttgcggc tatccgcaag acgcgggcgg
     2221 caacaaggct aaagccgaag aaatgagcac caacaaccgc gtggctttcc accgtatggc
     2281 gaacaccctc aactacctcg acatcaaaac cgtcgttgtc agttgcggca cttgttacga
     2341 ccagctcgaa aaataccgct tcgaggaaat cttccctggc tgccgcatca tcgacatcca
     2401 cgaatacctg ctcgaaaaag gcgtgaaact cgacggcgtg aaaggtcagc aatacctcta
     2461 ccacgacccc tgccacaccc cgattaaaac catgaacgcc acccaaatgg ccagcagcct
     2521 gatgggacag aaagtcgttt taagcgaccg ctgctgcggc gaatccggta tgtttgccgt
     2581 caaacggcca gacatcgcca ctcaggtcaa gttccgcaaa caagaggaaa tcgagaaaaa
     2641 cctcaaagag ctgccgcagg gcgaacccgt caaaatgctg acttcctgcc ccgcctgcct
     2701 gcaaggtttg agccgctacg ccgacgacaa caatatgcct gccgactaca tcgtcatcga
     2761 aatggcgaaa cacattctcg gcgaaaactg gctagacgag tttgtgaaaa aagccaacaa
     2821 cggcggcgtg gaaaaagtat tgctgtaaca acggacacgg aaatgtcgtc tgaacgccga
     2881 aagccttcag acggcattgt ttaaacccaa tacctaccca aatcatgatt gacggacaaa
     2941 caaccgaacc caaacaaaaa acccgaatca tccttgcccc tatgcagggt ctggtcgatg
     3001 acgtgatgcg cgacctgctg acgcgtattg gcggctacga cgaatgcgtc agcgaatttg
     3061 tacgcattac ccataccgtg cattcccgat ccatatggtt aaaatatgtc cccgaaatcg
     3121 ccaacggaaa caaaacgttt tccggcacgc cctgcaccgt ccaacttttg ggcagcgatg
     3181 cggacaatat ggcggcgaat gcgctggaag ccgtccgctt cggtgcgaac aaaatcgatt
     3241 tgaacttcgg ctgccccgcg cccaccgtca acaaacacaa aggcggcgca atccttttaa
     3301 aagagccgga actgatattc cacatcgtca aaacgctgcg cggacgtttg cccgcacata
     3361 ttccgctcac cgcaaaaatg cggctcggtt acgaagacaa aagccgggct ttggaatgcg
     3421 cctgtgcgat tgccgaaggg ggcgcatgcg gactgaccgt acacgcgcgt accaaagccg
     3481 agggttacga accgccggcg cattgggaat ggataaggaa aatccgagac agcgtcaata
     3541 ttcccgttac cgccaacggc gacgttttca gcctgcaaga ctatatcggc atcaaaacaa
     3601 tcagcggctg caacagcgtg atgctcggtc gcggcgcggt catccgcccc gatttggcgc
     3661 ggcaaatcaa gcaatacgag aacggcgggc cggtcaaaga cacggatttt gccgaagttt
     3721 ccaaatggat acggcagttt ttcgagctgt gcctgacaaa agaggcaaac aataaatacc
     3781 cgatcgcccg tcttaagcaa tggttgggca tgatgaaaaa aacgttcgac ccggcgcaaa
     3841 cgctgtttga ccgcgtccga acggttaagg atgcggatga agtccgccga atcctgaacg
     3901 catttgagca tgagataaat gtttgactgt ctcccatgct tcaaagcaaa tgccgtctga
     3961 aaatctttca gacggcattt catatcttta ttgctgcttt tcttccgtat ccggcgtatt
     4021 tttgtttttc agcttcgcac ccaagcccaa acgtttttca taatccgatt gcggggtatc
     4081 gtcttcctgc ataccgaatg cgccgacatt gacccacagc gacagcagcg cgacgacaaa
     4141 ggagcaaaag ccgattacat accaaaacat tgcccctccc gatttgttaa aatcatatca
     4201 aatacagtgc cgaatttatc acaaatgcac gggcaaatat agtgaattaa atttaaataa
     4261 ggacaaggcg gcgagccgaa gacagtacaa atagagacct ttgcaaaatt ccccaaaatc
     4321 ccctaaattc ccaccaagac atttagggga tttctcatga gcaccttctt tcaacaaacc
     4381 gcccaagcca tgattgccaa acacatcgac cgtttcccgc tattgaagtt ggaccaggtg
     4441 attgattggc agccgatcga acagtacctg aatcgtcaaa gaacccgtta ccttagagac
     4501 caccgcggcc gtcccgccta tcccctgttg tccatgttca aagccgtcct gctcggacaa
     4561 tggcacagcc tctccgatcc cgaactcgaa cacagcctca ttacccgcat cgatttcaac
     4621 ctgttttgcc gttttgacga actgagcatc cc
//
LOCUS       DAWHXZ010000164         4599 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000164 DAWHXZ010000000
VERSION     DAWHXZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.164"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            101..226
                     /locus_tag="WIE35_001666"
                     /pseudo
     CDS             101..226
                     /locus_tag="WIE35_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(377..1420)
                     /locus_tag="WIE35_001667"
     CDS             complement(377..1420)
                     /locus_tag="WIE35_001667"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ4154555.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSKAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(1417..1710)
                     /locus_tag="WIE35_001668"
     CDS             complement(1417..1710)
                     /locus_tag="WIE35_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154556.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQAVGQRSDGIPANHTKEKP"
     gene            1964..3118
                     /locus_tag="WIE35_001669"
     CDS             1964..3118
                     /locus_tag="WIE35_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ4154557.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDPRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTATQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKYEEVRKLIYQLIELDFLEIL"
     gene            3347..3700
                     /locus_tag="WIE35_001670"
     CDS             3347..3700
                     /locus_tag="WIE35_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154558.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRNWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            3761..4387
                     /locus_tag="WIE35_001671"
     CDS             3761..4387
                     /locus_tag="WIE35_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ4154559.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ILQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRATNLFIS"
     gene            4339..4539
                     /locus_tag="WIE35_001672"
     CDS             4339..4539
                     /locus_tag="WIE35_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154560.1"
                     /translation="MVSSDLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADI
                     LHEKRRLYEWVLELIYSMSGKL"
BASE COUNT         1257 a         1028 c         1184 g         1130 t
ORIGIN      
        1 cccgcgcagg cgggaatcta gaaatgaaaa accacggtgt tatcggcaat gactgaaacc
       61 ggagaaatga cgaaattgaa gcgtgtggaa atgtgtcgga aatcgctgaa gccggataaa
      121 ctatagtgga ttaaatttaa atcaggacaa ggcgacgaag ccgcagacag tacagatagt
      181 acggcaaggc gaggcaacgc tgtactggtt taaatttaat ccactataga ttcccgcttt
      241 tgcgggaatg acggtatttt agatttttgt tttcgcggaa taatgagcca aaagccgtcc
      301 gaacccaaaa aaaccgtcgc tttcagacgg catcaaaaaa ccgcaggcac gatgcctgcg
      361 gtttgccgtt tgcgcttcag gggagcaggg ggattgcgcc caagccgtgt gtttccttca
      421 gtccgaacat gatgttcata ttttgcaccg cctgacccgc cgcgcctttg acgaggttgt
      481 cgatgacgga aaggacgacc cacacatcgg attgcgccgc ctgttggatg ctgatgcggc
      541 agaggtttgc gccgcgcacg ctgcgggttt cgggtgtcga accggcgggc aggatgtcca
      601 cgaacaggct gtcgcggtaa tagtcgcgca ggatggtttc ggggcagatg ccgtctgaaa
      661 ggtggaggta aacggtggcg tgcataccgc gtatcatcgg cgcgaggtgc ggcgtgaaca
      721 cgaatccttc ggcgatgccg tcctgaagcc cggcgatggt ctgcctgatt tcgggcaggt
      781 ggcggtgtcc agctatgccg tatgctttga agttgtcgcc gacttcgcac aacagcgaac
      841 cgacattgcc tttcctgccc gcgccggaca cgccggattt gcagtcggca atcagcggca
      901 taccgggctt cagacggcat tgccgcaaca gcggcacgag cggcagggat acgcaggtcg
      961 ggtagcagcc ggggttggcg acgaggcgtg cctgtgcgac ggcttcgcgg ttgagttcgc
     1021 tcaatccgta cacggcttgg gaaacgaggt cgggggcggc gtgggtcatg ccgtaccagt
     1081 gttcccaggt cggaatgtcc cgtatgcgga agtcggcgga aaggtcgatg acgcgcacgc
     1141 cctgttcaat caggcgcggc gcgtctttca tggcgatgcc gttgggcgtg gcgaagaaga
     1201 cgatgtcgca ttgttccaaa cctgcctcgt cgggcgtttg gaaggcgagg ccgtacacgc
     1261 cgcgcaaact cggaaagtaa tcggcaactg cagttcccgc tttgctgcgg ctggttacgg
     1321 cggcgacttc gacatcggga tgggcggcaa gcaggcgcag cagttccacg cccgtgtagc
     1381 ccgtcgcgcc gacaatgccg actttgattt ttttactcat ggtttttcct ttgtgtggtt
     1441 ggcgggtatg ccgtccgaac gctgtccgac ggcttgtccg acggggtttt gattggcggc
     1501 ggaaggacgg tacacaaagc gtttgaaaag cgtgtctaat ttaatggcat ccgcctctgt
     1561 ttttaaaacc cagccctgcc gtccggaata aacataaccg taggcgcgaa agtcggcagg
     1621 atatttcggt gcctgcccca aacccaaacc gtatgcggcg aaagcggtgg tcgtggagaa
     1681 ggaaagcagg aggaggacga gggttttcat aaggttccaa accgaacggg gcgatgaagc
     1741 ccgattatag caaaaggtcg tctgaaaacc gttttcagac gaccttttat ttaattacaa
     1801 taaaaatgtt aacaacaaaa aacaaaccgc ttttttccgt ttggagacaa atttttaact
     1861 aaaattgtca acaattcctt gacgcacaca caaaaaaaac tttaatattt gtaaatatat
     1921 gtaacattat aaattttaaa tattttctga gtaaggggaa gtaatgagca tccatctcga
     1981 cttcggcatt agtcctaaaa cgttccgaca gacttatctg tatcaaaagc ccaagctctt
     2041 taaaggagcg gttcggaatc tcgaatccgc atcttggaaa gatatcaacg agatatacca
     2101 acgagcagac ccaaccgcac cgctgtttca tctgcgtaaa aaaggcgcaa tcgttcctaa
     2161 agaagaatac gtcgaaagtt tcgacgattt gggcaaaact cgctaccgtt ttattaaatc
     2221 cgttatctac gaacatatga agaatggtgc gtcgttagtc tataaccata ttaacaacga
     2281 gccgttttca gaccatatcg cccgtcaagt cgcccgcttt gccggcgcac atactattgt
     2341 tagtggatat cttgcttttg gcagcgacga atcttataaa aaccattggg acccccgcga
     2401 tgtgtatgcc atccagcttt tcggcaagaa acgttggcaa cttactgccc ctgatttccc
     2461 tatgccattg tatatgcaac agactaaaga tactgatatt tccattcctg aacatatcga
     2521 tatggatatt atccttgaag caggtgatgt cctctacatc ccacgcggtt ggtggcacag
     2581 acctatcccg ctcggctgtg aaaccttcca cttcgctgtc ggtaccttcc caccaaacgg
     2641 ctataattac ctcgagtggc taatgaagaa atttcccacc atagaaagtc tgcgccacag
     2701 tttctcagac tgggagcaag ataggacgcg tatcaacgat actgccacac aaattgctgc
     2761 catgattgcc gaccccgtca attatgaagc cttcagtgaa gactttctcg gcaaagaacg
     2821 taccgatacc gcttttcatc tcgaacagtt cgcgaatccc aacgctactc cgctttcaga
     2881 cgacgtcagg ttgagattaa atgccaataa tttggatacg ttggaaaagg gatatttgat
     2941 tgggaatggg atgaagataa gcgtagatga gttggggaaa aaagtgttag aacacatcgg
     3001 taagaatgaa ccgttattgt tgaaaaatct actggttaac ttcaatcagg gaaaatatga
     3061 agaagttagg aagttgattt atcagttgat agagttagat tttctggaaa ttttgtgagg
     3121 gattctatga aaaactggaa gcaatttaca tttttcgtaa tattagtaat agcttgttat
     3181 caagtgctat attttttatc agatatgttt cttctcgact acattaacaa atatagttgg
     3241 aatttgaatt ttattcaggg tactttgaat tttttttcaa tatatctgcc atatgttttt
     3301 gttagccgaa tttttaggaa taccaaccaa gaaaaggagt ataaaaatga ttgaacttca
     3361 acttcatgaa ttgaagctgg tttcaggggg aggtcctgta acagacaata tagctggaaa
     3421 tgtagctaat gctgccacaa ccaaaggagg tcccacatgg ggggatttgg ttgcaatacc
     3481 tgctgccgca gcggcagttt attatctgcc gaaaaatgct tacggtgctg ctggtgcaaa
     3541 tggtgtatac aatgtgactc gtaattgggt aaatgatgcc gttaatgcac ctccttataa
     3601 cggaagacca atctttgaga ttgaacatgg attaactgct cccgcaacaa aagcagataa
     3661 atcaggaaac ggctacactg acggtacaga ttactgctga tatttctcat cgtccagaca
     3721 atctctaggg gtcgtctgaa actttcttaa cttcaatttt atgaatagac ccaagcaacc
     3781 cttcttccgt cccgaagtcg ccgttgcccg ccaaaccagc ctgacgggta aagtgattct
     3841 gacacgaccg ttgtcatttt ccctatggac gacatttgca tcgatatctg cgttattgat
     3901 tatcctgttt ttgatatttg gtaactatac gcgaaagaca acagtggagg gacaaatttt
     3961 acctgcatcg ggcgtaatca gggtgtatgc accggatacg gggacaatta cagcgaaatt
     4021 cgtggaagat ggagaaaagg ttaaggctgg cgacaagcta tttgcgcttt cgacctcacg
     4081 tttcggcgca ggaggtagcg tgcagcagca gttgaaaacg gaggcagttt tgaagaaaac
     4141 gttggcagaa caggaactgg gtcgtctgaa gctgatacac gagaatgaaa cgcgcagcct
     4201 taaagcaact gtcgaacgtt tggaaaacca gaaactccat attttgcaac agatagacgg
     4261 tcagaaaagg cgcattagac ttgcggaaga tcagtggcaa aaacggcact gggcagacag
     4321 gaattgtcgg gattgggcat ggtatcctcc gatttggcga agagcaacga acctgtttat
     4381 ctcgtgaaaa taaaacccga caaaccaacc atcactgcat acggtgagga aaaaccgctg
     4441 caaatcggca tgacgttgga agcagacatc ctacacgaga aacggcgatt gtacgaatgg
     4501 gtattggagc tgatttatag tatgtcgggc aaactgtaag acttgaggct gtaaagataa
     4561 agaagttgtc tgaaaccttt tcagacgacc agaagacaa
//
LOCUS       DAWHXZ010000165         4586 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000165 DAWHXZ010000000
VERSION     DAWHXZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.165"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            84..308
                     /locus_tag="WIE35_001673"
     CDS             84..308
                     /locus_tag="WIE35_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ4154561.1"
                     /translation="MLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMTRL
                     PTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            complement(516..1901)
                     /gene="pntB"
                     /locus_tag="WIE35_001674"
     CDS             complement(516..1901)
                     /gene="pntB"
                     /locus_tag="WIE35_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ4154562.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGINGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1967..2350)
                     /locus_tag="WIE35_001675"
     CDS             complement(1967..2350)
                     /locus_tag="WIE35_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ4154563.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(2354..3895)
                     /locus_tag="WIE35_001676"
     CDS             complement(2354..3895)
                     /locus_tag="WIE35_001676"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ4154564.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWVCPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKDVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFFVTRRMLN
                     MFRKG"
     gene            4185..>4586
                     /locus_tag="WIE35_001677"
     CDS             4185..>4586
                     /locus_tag="WIE35_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ4154565.1"
                     /translation="MPHALVLQFPSAETLPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEKDAALLGSRQIDHAVLSDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILA"
BASE COUNT         1011 a         1167 c         1321 g         1087 t
ORIGIN      
        1 aactctacca aagcgacggc agacacagcg tcaatccgac tgagggctac ggcacaggcg
       61 gcttgttgca aggcaaaaaa catatgcttt cactgacttg gaatgcgccg attgaggcgt
      121 ttacccgcga aggcgatttc tttgagggca aaggcgttga tgttttgtat atgcacttcc
      181 acaaagccaa cgagtttttg ggtatgaccc gcctgccgac attcttatgt aacgatgtgg
      241 ttaaaaatcc gcaagtggaa aaatacttgg cagattatca ggcacacttg gaaaaagtgt
      301 tcggctaatt aaaaatccat cttcaacacg gagatggatt ttgtttgttt cgttgatttt
      361 gtgtcagttt cagatgtagg tgcttattcg gacgggccgt ctgaaaatgt ttgccccaat
      421 gcaaaaaaat cactgcaaac cttcataaac ggggtttgca gtgatttttt caaatcaaac
      481 agattgaaaa cctgcgccga attgttcaga cggcattatt ttttcagttc ggacagaatg
      541 tcatctacgg ttttcttcgc atctccgaaa cacatcacgc tgttttcgtt gaagaacagt
      601 gggttttgta cacctgcgta gccggtattc atcgagcgtt tgaagacgac gacttctttt
      661 gccttccaca cttccaacac gggcataccc gcaatcgggc tgttcgggtc ggtttgggcg
      721 gcggggttga cggtgtcgtt cgcaccgatg accaagacca catcggtttc ggggaagtcg
      781 tcgttgattt cgtccatttc caaaacgatg tcgtagggga ctttggcttc ggcgagcagt
      841 acgttcatat gaccgggcag gcggccggcg acggggtgga tgccgaagcg tacttcggtg
      901 ccgtttttac gtaaaagctc ggtgatttcg gcaacggggt attgcgcttg tgcgactgcc
      961 ataccgtagc ccggggtaat gatgacattg tttgcgcctt tcagcatttc ggcaatatcg
     1021 gcagctttga cttctcggta ttcccctatc tcttggctgc cggaagataa tgtgccgctg
     1081 tcgctgccga aaccgccggc aattaccgag acaaacgagc ggttcatggc tttgcacata
     1141 atgtaggaca gaatcgcgcc gcttgagccg accagcgcgc cggtaacgat gagcaggtcg
     1201 ttggagagca tgaagcctgc cgctgcggcc gcccagccgg agtaggagtt gagcatggac
     1261 acgaccacgg gcatatctgc gccgccgatg gaggcaacca agtgccagcc gaatgcgagg
     1321 gcaatcaggg tcataatcag caggatgaag ccgctaccgt taatgccgac gaatacgagc
     1381 agcaacacaa acgatacggc gagcgccagt gcgttgagct tgtgtttggc gggcagttgc
     1441 agcgggctgc tgctgatttt gccgttgagt ttgccgaatg cgaccagcga gccggtaaag
     1501 gttaccgcgc cgatgaaaat gcccaaatag acttcgacca gatggatggt gtgcatatcg
     1561 tgcgaaacgt tgcccggctc gatatagctg ttgaagccga ccaaaaccgc cgctaggccg
     1621 acgaagctgt gcagcagggc aatcagttcg ggcatttcgg tcatttccac ctttttggct
     1681 ttgtagatgc cgattgccgc gccgatgagc atggcgatga tgatccagcc cagtccgtgg
     1741 gtattgtcgg aaaaaacagt tacaaaaagg gcgaccgcca taccggcgat accggaatag
     1801 cagccctgtt tggcggtttc ctgtttggac agccccgcca gtgagaagat gaataaaatt
     1861 gcggcaacga tatacgccgc tgttacgagt cctgaagaca tggaaattct ccgattttcg
     1921 atgatttgtt tttaatgccg tctgaactgt tcagacggca tctgtttcaa gcagccgcga
     1981 caaacagccc gacggtgcag gcaaatccgc ccgcccacat cagcgagcgc aatgctgctt
     2041 tgtctgcgat gtagcaccag ataaaggcga ggcggaacag gatgaacagg ccggcaagcg
     2101 tgttgacggt tgcttgtccg gcattgccgg ttgcgtgtgc cgtcaaaacg gcggcggcaa
     2161 acggtgcaaa ggcttcaaaa ccgttttgct gcgcggcgtg ggcacgggcg gctgtgcctt
     2221 gcgtgcgcgc cagaaaatcg cgcggattgt ggttgtcttt aaaccggaat ccgcccgctt
     2281 ttttggcata tgccgcacaa aaaagcggca ataagcaggc aatcaggatg caccaatagg
     2341 caaaagtcat gctttatcct ttcctaaaca tattcaacat gcggcgcgtt acgaagaagc
     2401 cgccgaagat attgatgctg gcaatcagga tggcgatgaa ggcaagcagg gtaacgaaac
     2461 cgttgccttg gctgatttgc agcagcgcgc cgacgacgat gatgccggag atggcgttgg
     2521 ttaccgacat cagcggtgtg tgcagcgagt ggctgacgtt ccaaacgaca tagtagccga
     2581 tgacgcaggc gaggacgaag acgataaagt ggttcaggaa tgctgcgggt gcgaccgcgc
     2641 cgacccacag taccaacacg gcggcgatga cggcgggcgc gagttttttc cacaggggaa
     2701 cgggtttcgg ttcgggcttg gcggcaggcg cggctttttc agacggcgtt tgctgcggtt
     2761 gggcggaaac ttgaatcggc ggaggcggga aggtgatttc gccgtcgcgg gtaacggtca
     2821 tgttgcggat aatcacgtct tcaaaatcca gcgtgatttc gccgtctttg ttcgggctta
     2881 acaacttggt caggttgacc aggttggtgg cgtaaagctg ggaagactgt ccggcaaggc
     2941 ggtttgccat gtcggtgtag ccgatgattt tcacgccgtt gccggttacg gacaattcgc
     3001 ccggtcgggt gagttcgcag ttgccgcccg tcgccgccgc caaatcgacg atgacggagc
     3061 cggatttcat gctttccacc atttctttgg taatcagctt gggcgcgggt ttgcccggaa
     3121 tggcggcggt ggtgatgatg atgtccacgt ctttcgcctg ctcggcaaag agcttcatct
     3181 cggcggcgat aaattcgtcg ctcatcactt tggcatagcc gtcgccgctg ccgcccgatt
     3241 cttgcgggaa gtcgagtttc aaaaacttgc cgcccatcga ttcgatttgt tccgccactt
     3301 ccaagcgggt atcgaacgcg cgcaccactg cgccgagcga gtttgccgta ccgatcgccg
     3361 ccaaacctgc cacacctgcg ccaatcacca aaacctgcgc gggcggcact ttgccggcgg
     3421 cggtaatttg accggtgaag aaacggccga aggcgttggc ggcttcaatt acggcgcggt
     3481 agccgctgat gtttgccatc gaagacaaag cgtccaaagc ctgcgcgcgc gaaatgcggg
     3541 gcaccatatc catcgccagc gcgttcactt tcttggcgcg caaggcttcg accaaagcct
     3601 cgttttggcg cggccacagg aagctgacga tggtttgacc ttcgttcaaa agcggcagtt
     3661 cctgttcgga cggcgcgttg accttataaa tcaaagggca gacccaaacc gccgctttgt
     3721 cggcaacggt tgcgcctgct gtttggtaag cggcatcgtc caaacttgcc gccaaacctg
     3781 caccgctttc gacaacggtt tcaaagccca gtttgcccag cagggcgacg gtggcgggcg
     3841 tgcaggcgac gcgggtttcg ccggataatg actcgcgtgg gataccgatt ttcatctctg
     3901 aatccttttt cgggtagttt atatgtatcg tgggttaaat ttaaatcggg gcagggcgga
     3961 gcaacgccgt accggtttaa aaccgattca cttcaaatgt taatatattt tagataatcc
     4021 ccttataacg aattttcatc aggctggcaa tagttgcggc attttcccgt gttgtccgac
     4081 acatactgcc gtttgcgttt gaagtcattt tccgattgca gtcccgcccg ccgtaacatt
     4141 ctttgccgtt tcactatata atccgcattt tttgagccgc cgttatgccg cacgccctcg
     4201 tcctccaatt tccctccgcc gaaaccctgc cttccgactt cccctcacgc ctgcccgaac
     4261 ctgattatgc tgatgaaaag cgtatgcgtt ttatcgttga agaagggttt tctttaagcg
     4321 aaaaagacgc ggcgttactt ggcagccgtc aaatcgacca cgccgtgttg tcggatatgg
     4381 attttggcga actcggtttg attgtcagcg atatggattc gacgctgatt accatcgaat
     4441 gcgtcgatga aattgcggca ggcgtgggtt taaaagacaa ggtagcggaa attaccgagc
     4501 gttcgatgcg cggagagttg gatttcgagc agtcgttgcg cagccgcgtt gcgctgttgg
     4561 cgggattgga cgaacagatt ttggcg
//
LOCUS       DAWHXZ010000166         4524 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000166 DAWHXZ010000000
VERSION     DAWHXZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.166"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(125..1063)
                     /locus_tag="WIE35_001678"
     CDS             complement(125..1063)
                     /locus_tag="WIE35_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ4154566.1"
                     /translation="MLELNGLCKRFGNKTVADDICLTVGRSKILAVLGRSGCGKSTLL
                     NMIAGIIRPDGGEIRLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAESLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVAHLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMRYAGAVSGKDTVRIHIEEREIVRFR"
     gene            complement(1072..1947)
                     /locus_tag="WIE35_001679"
     CDS             complement(1072..1947)
                     /locus_tag="WIE35_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ4154567.1"
                     /translation="MPTVRFTESVSKQDLDALFEWAKASYGAKSCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWTDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNTAAGGVSSGELPSETVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAILPETFLPENQDGEVACFEKMDIGGLLDAMLSENMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2136..2212)
                     /locus_tag="WIE35_001680"
     tRNA            complement(2136..2212)
                     /locus_tag="WIE35_001680"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2176..2178),aa:Met,seq:cat)
     regulatory      2362..2461
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2554..4455
                     /gene="thiC"
                     /locus_tag="WIE35_001681"
     CDS             2554..4455
                     /gene="thiC"
                     /locus_tag="WIE35_001681"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ4154568.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTRPDQITPEFVRREIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1089 a         1289 c         1201 g          945 t
ORIGIN      
        1 gaatgacggc agggtggttt ctgttgtttc ggataaattt tgcagccctg ataaaaaata
       61 tggctgcttt ggtaaaaaaa tgccgtctga aaggttttca gacggcattt gtttttaaga
      121 agcatcagcg gaagcggacg atttcccgtt cttcgatatg gatgcgtacc gtatccttgc
      181 ccgataccgc cccggcgtac cgcatatcga ggttcagcca taggatgccg tgttccggat
      241 ggaggacgga caggctgaac gattcgggca aacaggtacg ggataatacg cggcactcca
      301 tgccgtcttg gtcgaaacgc accgcatgtt gcggaatatg gcggtcatcg tcggtattgg
      361 gcagccccat cagatgggcg acctgcacgc cggcaggcgt ttgaaccaag gtttcgggcg
      421 taccgcattg aaggattttc ccctcgtgca tgacggcgat ttcgtctgcc gccgtgcagg
      481 cctcttcggg cgaatgcgtt accaaaacgg cagggatgcc gcccttgcgg atacgttcgg
      541 cagtcatgcg gcgcagccgg tcgcgcaaat gcgtgtccaa actggaaaac gattcgtcca
      601 acagcagcag ggaagggcgg acaaccaaag cgcgcgccag tgccaaccgt tgcttttcgc
      661 ctccggaaag tttttcaggc ttgcggtgcg cctcgttttc cagtccgact tcggcaagtg
      721 ccgccatggc gaggctttcg gcttcggctt tcggcatttt ttgcattttc aaaccgaatg
      781 ccgcattttc cagtgcactc atatggggaa acagcgcgta atcttgaaac atcagcgaaa
      841 tacggcgttt ttcgggcggc atacgggtaa tgttttcccc gttcagccga atttcgccgc
      901 cgtccggacg gataatcccc gcaatcatat tcagcagggt ggattttccg cagcccgacc
      961 gtcccaaaac ggcaagtatt ttgctgcggc cgacagtcag gcagatgtcg tcggcgacgg
     1021 ttttattgcc gaagcgtttg cagagtccgt tcagttcaag catggcgtat cctataaacg
     1081 tatgccgtcc agccactcgg acagcggatg ggcggcatca atcagaccgt aacggcaaaa
     1141 cgcgtccagc gtaaccagtt gcgcgtcgtg catcatgttt tccgacaaca tggcatccaa
     1201 cagaccgccg atgtccatct tttcaaaaca cgcaacctcg ccgtcctgat tttcaggcag
     1261 gaaggtttcg ggcaggatgg catcgaatac atacaggatt tcattgtgca caccccggct
     1321 gacggggcgc aggctgtgca gccgagatac cggacggata aacgggaaca gcgttttatc
     1381 caaaccggct tcttcgctgc tttcgcgaca cacggtttca gacggcaatt caccgctgga
     1441 aacaccgccg gcggcagtat tgtcgagttt gtcgggatcg actgctttgt gcggactgcg
     1501 cctgcctatc cagaaatgcc atcggccgtc cgattcggtc agaccgttga gatggacggc
     1561 gcggctgagt agtccgaacg ggcggaaagc ggcgcgttcg agcgtgaaca aggggttgcc
     1621 gccgccgtcg gtcaggtcga aacactcgtt gcgccagccg tgaagcagcc ccgccttgtt
     1681 ccatgtgcgg gcgaggtgct gcaagcgtcc gcccatatcc gtccagccgt ccgcattcag
     1741 aaaaatgccg tctgaagact ccgagcagcc tgcctcccag tcttttttga cgcgctccgc
     1801 ccattccggc gacagattgc ccaaaggcag acggttcaga tacagcgttt tccagcaact
     1861 ttttgcaccg taacttgctt ttgcccactc gaacagggca tcaaggtctt gtttgctgac
     1921 ggattcggta aaacggacgg tcggcatagt cggctttctt gaacataatg cggaagattg
     1981 tagccaaacc gcctgaattt gcaaccgcaa acagacggcg ggctgccgtt ccgatattgc
     2041 ggcaggaagg aaaatccggc aaaaaacgga ggcggcagga aacaggaaac aggcaaaaca
     2101 aaacccctcg gcattgaacc gaggggttta atatttggtt gcgggagcag gattcgaacc
     2161 tacgaccttc gggttatgag cccgacgagc taccatgctg ctccatcccg cgtcagaaga
     2221 tggaactata cggcagattt tttatgttgt caaacattat ttccgcgaaa aatcataaaa
     2281 ttttgccgtc cggcggttta tgcctgattt gaaatatcat tttctttaca aaagttcatg
     2341 ttcgtgattt aattttggtt aacattgaaa caggggtgct gcatgatgtt caggcggctg
     2401 agaaataccc tttacacccg atctggataa tacctgcgtg gggagttttc acggattctg
     2461 cctttcagac ggcatttttt gatactcggg ccgtttgaaa aagcaaagtg ctcctgtttc
     2521 tttattctaa acgagaaaac aggagcattt tttatgacta cgccaaaaaa aactgccaaa
     2581 acttccggca acgaagcgcg cgaacttgcc gacttgagcg aagacatcgg catccgcttc
     2641 aaatatccga actcggagcg cgtgtatctg caaggcagcc gcgacgacat ccgcgtgcct
     2701 ttgcgcgaaa tccgtcagga cgacacctac acggcgcaag gcacggaagc caacccgccg
     2761 attcccgtct atgacaccag cggcgtgtac ggcgatccgg cggcgcacat cgacctgaaa
     2821 caaggtctgc cgcacatccg cacggcgtgg ctggacgaac gcggtgatac cgaaatcctg
     2881 cccaagcttt ccagcgaata cggcatcgaa cgcgcacacg atccgaaaac cgcccatctg
     2941 cgcttcaacc aaatcacccg cccgcgccgc gccaaagccg gccgcaacgt aacccagctc
     3001 cattacgccc ggcaaggcat tatcacgccg gaaatggaat ttgtcgccat acgcgaacgt
     3061 ttgaaattag acgaattgtc ccaaaaaccg gaatacgcca aactcttgaa gcaacacgcg
     3121 gggcaaagtt tcggcgcgaa catcccgacc cgtcccgatc aaatcacacc cgaattcgtg
     3181 cgccgggaaa tcgccgccgg acgtgcgatt attcccgcca acatcaacca tcccgaactc
     3241 gaaccgatga ttatcggccg caacttccgc gtcaaaatca acggcaactt gggcaattcc
     3301 gccgtcactt ccagcctgac cgaagaagtc gaaaaaatgg tgtggtcgct gcgttggggc
     3361 gcggacacaa ttatggactt atccacaggc gcgcacatcc atgaaacgcg cgaatggatt
     3421 atccgcaacg cgcccgtccc catcggcacc gtgcctattt atcaggcttt ggaaaaaacc
     3481 ggcggcattg ccgaagattt gacttgggat ttgttccgcg acaccttaat cgagcaggcg
     3541 gaacaaggcg tggactattt caccatacac gcgggcgtgt tgctgcgtta tgtaccgatg
     3601 accgccaacc gcctcaccgg catcgtctcg cgcggcggtt cgattatggc gaaatggtgt
     3661 ttggcacatc atcgggaaaa cttcctctac acgcatttcg acgaaatctg cgaaatcatg
     3721 aaagcgtatg atgtgtcgtt cagcctcggc gacggcctgc gccccggctg cattgccgat
     3781 gccaacgacg aatcccaatt cgccgagctg cacaccttgg gcgaattgac cgataaagcg
     3841 tggaaacatg acgtacaagt catgatcgaa ggccccggcc atgtgccgct gcaacgcgtc
     3901 aaagaaaaca tgaccgaaga gctgcaacac tgctttgaag caccttttta cacactcggc
     3961 ccgctcgtta ccgacatcgc acccggctac gaccacatca cctcgggcat aggcgcggcc
     4021 aatatcggct ggtacggcac ggcgatgctt tgttacgtta cccccaaaga acatttgggg
     4081 ctgcccgaca aagaagacgt gcgcaccggc atcatcacct acaaactcgc cgcccacgcc
     4141 gccgatctcg ccaaaggctg gccgggcgca caattacgtg acaacgccct gagcaaggcg
     4201 cgtttcgagt tccgttggcg cgaccaattt cgcttaagcc ttgaccctga acgtgccgag
     4261 agcttccacg acgaaaccct gcctgccgaa ggcgcgaaaa tcgcccactt ctgctcaatg
     4321 tgcggcccca aattctgctc gatgaaaatc acgcaggaag tgcgcgacta cgccgacaag
     4381 caaaaagccc aacggcaggg catggaggaa aaagcggtcg agttcgtcaa aaaaggcgcg
     4441 aagatttaca gttgaaacgt caagcaaaaa atgccgtctg aaacccggaa aaaaggcttc
     4501 agacggcatt ctttcgcttg tgaa
//
LOCUS       DAWHXZ010000167         4462 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000167 DAWHXZ010000000
VERSION     DAWHXZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.167"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1017..1787)
                     /gene="xth"
                     /locus_tag="WIE35_001682"
     CDS             complement(1017..1787)
                     /gene="xth"
                     /locus_tag="WIE35_001682"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ4154569.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPALPDDPQRRVIAATVSGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1847..2128)
                     /locus_tag="WIE35_001683"
     CDS             complement(1847..2128)
                     /locus_tag="WIE35_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ4154570.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSNHLNRLRVEGLVDFTRYHRIIEYRLVSEEAAAILHTVRDLENKRVA"
     gene            complement(2534..3415)
                     /gene="nadC"
                     /locus_tag="WIE35_001684"
     CDS             complement(2534..3415)
                     /gene="nadC"
                     /locus_tag="WIE35_001684"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ4154571.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVQQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTIYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(3491..4429)
                     /locus_tag="WIE35_001685"
     CDS             complement(3491..4429)
                     /locus_tag="WIE35_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154572.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
BASE COUNT         1031 a         1118 c         1191 g         1122 t
ORIGIN      
        1 ctgatgcaga tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
       61 caaatagtac ggcaacgctg tactggttta aatttaatcc actatatttt gtaatagctg
      121 ccattatgcc tactaggata tataccttct atataatcct tatttaaata aaaaccctta
      181 taaaaatctg tgttatctgt cgggattgaa aaaaagattt tatctttctc atcaggttta
      241 tatttgctat caatatcaaa aagagaataa ggattacaat gagttcgaca tactaggtaa
      301 aaatttatat cttggatcat gaaatattcg atatttatca ggatcgaaag atgctaaaaa
      361 ttcatcaata ttggtactgg atattttgtc atcactaata aacatcgcta gagatgggcg
      421 actttgattt tccatttgtt gattagtaaa ggatataaat actaacttgc cattgtcaaa
      481 ccacatagat ttatatagtg gattaaattt aaaccagtac gtcgttgcct cgccttgccg
      541 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatag
      601 ttattgcctt tgggagaatc aattctgaca gcacataaca taggttcatc tttacagtcg
      661 ttatattggg tttcatcaaa ctcaactgca tagcacaata aaggcaatag gaaaaaaagg
      721 agattaattt attactatac ataaggcagc ctgactattc attctaaaat aaatccggtt
      781 tttaatattt gattctgctc cggattatat acataataaa ttttactgct ccaaaatctg
      841 atattaaccc tatggatttc ataaattttt cagaaaaata ggctgtgctt gaacaatccg
      901 ccgactccgg ctgtttgtca gggaaaaggc aattattttc ggttagtcag cagcctatcc
      961 tattgaatca cttaaagata tggatgtttg tcaacgccat atcaaaacac ggtcttttac
     1021 caatcgaatt ctgccgtcac cggcgcgtgg tcgctcggac gctccagcgc gcgcgtctcc
     1081 aaatcgacgc ggacatcctt caacgccgcc gccatcgcag gcgacaccaa aatatggtcg
     1141 atacgcaggc ccagtttgcg ttggaacatc gcgccgcgat agtcgaacca ggtatagaac
     1201 gcgccttcgg gatggacttg gcgcaggctg tcggtcagtc ccaaatccag caggttttga
     1261 aaccactgcc gttcgacgga cgaacagtgg attttttcgt gccatttttc agggtcgtaa
     1321 cagtccgcat cggcaggcgc gatattgaaa tcgcccagca acaccagttt gccgtggcgg
     1381 gtcatttcat cgcggacaaa ctccgtcagt gcggcaaacc actgttcctt atatttgaat
     1441 ttggggctgt ccaaagcctc gccgttgacg caatagacat tgatgacgcg cacgccgctg
     1501 acggttgccg caatcacgcg ccgttgcgga tcgtccggca gtgcgggcaa accgaaatgc
     1561 acgtcctgcg gcacgctgcg gctgacgatt gccacgccgt tgtaggtttt ctgcccgctc
     1621 caaacacagt gccagcccat catttgcaaa gcggcggccg gaaatttgtc ctgatcgagt
     1681 ttgagttcct gcaaaaccaa aatatcgggc ggattgtcgg caagcaggtt ttgcacctgc
     1741 ggcagccgca cattgagcga attgacattc caagtggtga ttttcataac atttccgaaa
     1801 aatgctgtct gaaacgttca gacggcaaaa ggaaaggatt ctaacactat gccacgcgtt
     1861 tgttttccaa atcgcgaacc gtgtgcagaa tcgccgccgc ttcttcggaa accaggcggt
     1921 attcgataat gcggtggtaa cgcgtaaaat cgactagacc ttccacgcgc aggcggttca
     1981 aatggttgga aactgcggtg gccggcaggg ataaggattt tgccagttcg gcgatattgc
     2041 gttcgctgtc caacaattga atcagtatcg ccatacgttc gggatgcgcc ataagtttga
     2101 gtatggtgtg gagcggtatt gtgttcatcg gtattcctaa aacctttgcc gcccgccaag
     2161 cggattgacg ggcggcagat atggatgatt gtgattatct ttataaatcg tattataaat
     2221 caaatattta tggatgattg tacaggattg tatgttcctt ttcccacttt tttgcaaaca
     2281 agcgtaaagg cgggctttca gacggcataa aactgccgcc ccccgtcctt tttcctgccc
     2341 taccgatgcc gccgcgctgt cgggcaaaca aaatacctgc ctgaaatgct gttttttcta
     2401 aagcatcacg acctttacga aaaatgccgt ctgaagaatc tttcagacgg catttcctcc
     2461 ctgaggcaat aaccggatta gggttaagcg gttccttgcc tgacatatca gacagccgcc
     2521 cgcaccctaa aactcacgcc acgaaatcca aacctatgtc caacgaacgg ctgctgtggg
     2581 tcagatagcc gagggcgatg ccgtccactc cggtttgcgc cacgcgcttc aggcggtcga
     2641 agccgatgcc gcccgatgct tcgcaataga tggtgtgggg gtgggcggtt tgcgtgtggc
     2701 agcggtttgc cgcttctttc agggtttcgt cgtccatgtt atccagcaaa atccgttccg
     2761 cgcccgctgc gatggcttcg tccagttgtg ccaacgtatc cacttcgatt tccacgcagg
     2821 tcaatgctcc gacagcctgt tttgcctgct gcacggcttg ggcgatgctg ccgcaatagg
     2881 cgaggtggtt gtctttgatg agcacggcgt cgtccaaacc catgcggtgg ttcacaccgc
     2941 cgcctgccct gacggcgtat ttttgcagga cacgcagcag ggggatggtt ttgcggctgc
     3001 acacgatgtc tgtaccgtat tcggcgactt cggcaacggc acgcgcggtg gcggtggcga
     3061 tgccgcttaa gtgcgtgagg tagttgagcg cggtgcgttc ggcggcgagc agcgcgcggg
     3121 cgttgccttc gacggcggca agcgtctgac ctgcgcggac ggcttgcccg tctcggattt
     3181 cggcttggaa gcggacggac ggatccatcg tctgaaaggc gaggcgcgct aaatccatgc
     3241 cggcgataac gccgtcttcg cggctgacga ggaagagttt ggcggttttg tcgggggcga
     3301 taacggcggc ggacgtaatg tcgccgcgcc tgcccaagtc ttcgctcaag gcttgttcga
     3361 ctatggggcg caacagggtg tcgggcaggg aaaagagggt gttttcagac ggcatgaggt
     3421 ttcctttgtc gaaaaagatt gatgttgcac cgatggggca aaacttcatt tgatgccgtc
     3481 tgaaaacggg ctaacggctg cccagcggca atccgatgtc cgaaatcagc ctgtcgggca
     3541 ggacgtcgtc gcggaagcgg caaagctgcg cgggacggcc tttgctgccc gatacgccgg
     3601 tatccgacgg ctcgataagg ttttgctgct gaatctggcg gcggaagttt tgcttgtgca
     3661 gcaatcttcc gctgatggct tcgacactgt tttgcagttg cagcagcgtg aattcgggcg
     3721 gcatcagttc aaaaatcacg gggcggtatt tgattttggc gcgcaggcgg gacagggcgg
     3781 tcgccagtac gcgacggtga tcgtggcgca tgggctgccc cgtgagcgcg aagtcgaagt
     3841 ttgcctgcgg ctcggcggct tccactatca gaccgctttc atacagcatt tcatagcgtt
     3901 gtaaaacgta ttcttccgac cagttttccg gttcgacccc ccagcacaaa tgaatgcgct
     3961 tgagccgcat ttgacgcact tcctccgtgt ccgccgaatt tgcccaaatg cgcaggcggc
     4021 tgacgacggc gtcgcgctgc ccgccgtcgg tgcgcaagtc ttcccacggg aaatagccgt
     4081 agcagtcctg ccatttcgca tccgggtgca ggatgctgtc ggctgcctcg cgcaccagcc
     4141 ccaaatagct gacgtacaag acgggcatgc cgtgttcgtt gcggcggtgg gtatcgacaa
     4201 aggtgtaaag ctgttccaca tagcccatag gctgcgaagt ctgcttggca acccacagct
     4261 tcacgcccgc ctgcagggaa ttgcgcagag gggagagcgg gccgttgggc aggagtgtgc
     4321 cttgggcgac ggttaatacc cgcaggccgc cgtcggtaac ggcaatcaat acgggaacca
     4381 gctcgacgat gctttgcggc ggggcttcgg cttcggggta ggcatccatg ctcggctttc
     4441 tgcgggtggg aaaggcttta tt
//
LOCUS       DAWHXZ010000168         4386 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000168 DAWHXZ010000000
VERSION     DAWHXZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.168"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(46..3078)
                     /locus_tag="WIE35_001686"
     CDS             complement(46..3078)
                     /locus_tag="WIE35_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEZ4154573.1"
                     /translation="MFWIVMIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPNASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNGAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIYIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPAEFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFENVPSERPSCRVSDTEADE
                     GAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGSGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3243..3923
                     /locus_tag="WIE35_001687"
     CDS             3243..3923
                     /locus_tag="WIE35_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ4154574.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSVGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3952..4311
                     /locus_tag="WIE35_001688"
     CDS             3952..4311
                     /locus_tag="WIE35_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ4154575.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
BASE COUNT          888 a         1288 c         1117 g         1093 t
ORIGIN      
        1 tgccgtctga aacagtcttc agacggcatt tccatttgca aaaaatcaag cattgtccaa
       61 ggggacgaga atcgtacggt tgccgttgtg ttccggtgcg gacacaatgc cttccgcctc
      121 catctggtca atcagacgcg cggcgcggtt gtagccgatg cgcaaggcgc gctgtacgcc
      181 cgaaatactg gctttgcgcg ttttcaggac aacggatacg gcttcatcat acatcggatc
      241 ggtttcgtcg tcgccgctgc gcccgatgcc gggcagctct tcgctgccgc tgccgctcaa
      301 aatatcgtca acatagtccg gctcgccaaa ctgtttcaaa tattcgacca cgcggtgcac
      361 ctcttcatcc gaggcaaacg cgccgtgaac gcgctgcgga taggcagtac ccggcggcag
      421 gaacagcata tcgccctgac cgagcaggtt ttccgcgccc atttggtcga gaatcgtgcg
      481 gctgtcgatt ttgctggaca cttggaacgc gatacgcgtc gggatgttcg ccttaatcag
      541 acccgtgatg acatcgacgc tggggcgttg tgtggcaaga atcaaatgga tgcctgccgc
      601 gcgggctttt tgtgcgaggc gcgcaatcag ttcctcgatt ttcttgcctg ccgtcatcat
      661 caaatcggca aactcatcga ccacgaccac gataaacggc agtttttcca aaggttcggg
      721 atcgtcgggc gtgaggctga acggattgcc gattttttct ccccttgctg cggcttcggc
      781 gattttttga ttgaagcccg caagattacg cacacccata aagctcatca ggcggtagcg
      841 tttttccatt tcgttaacac accagttcag cgcgtttgcc gccagcttca tatcggtaac
      901 gacaggggcg agcaggtgcg ggatgccttc gtaaatgctc aattccagca ttttcggatc
      961 gatcataatc atacgcacgt cttccggcgc ggctttgaaa agcatagaca gaatcatcgc
     1021 gttgacaccc accgatttgc ccgaaccggt cgtgccggca accaacaaat gcggcgcttt
     1081 tcccaagtcg gttacgacgg gctgtccggt gatgtcctga ccgagcgcga gcgtcagctt
     1141 ggatttggat tcggcaaact cgggcgaatt gaagatttcg ctcaggcgta tcatttggcg
     1201 tttcgggttc ggaagttcca aacccataca ggttttgccg gggatggttt cgacaacgcg
     1261 gatggaagcc acgccgagcg aacgcgccaa gtctttttcc agattcagaa cggaattgcc
     1321 gcgcacgccg acatcgggtt cgatttcata acgcgtaatc acggggccgg aataagaatc
     1381 gacaaccttg accttgactt tgaactccgc caatttttct tcgatggtga tgctgttttc
     1441 caacagttct tcttcggttt gcgtcgcctc gggattgaac agcggcggca gaagcaggtc
     1501 ggttgtcggc aggtgttcgg ataccgcacc ggtctcttcc gattggaacg ccccttcatc
     1561 cgcttccgta tccgataccc ggcaggacgg gcgttcagac ggcacatttt caaacggaca
     1621 aaccgcctga ctgtcatgcc cgaacgcttc ggtttccgac agatattgcc cgcttgaccg
     1681 ctctgccgca ccttcactgc cgtcatctgc aatagcggcg gtttcctcct gccaacctcc
     1741 attcaaaaca tcatcggcaa gatggtcggt ttcggcaatg cggctgcgtt gcgcctgctc
     1801 gaattctgcc ggcggttcat aggtacggtt gtagatttcc gatacgggag gcggcggcgg
     1861 aatatcgatt gcgggcatgg gaactttcgg cacttcgggt ggttcgacca cccaagcatc
     1921 gggcgcagca ggttcttcaa tatagatatc ggcagcttca tcataaaccg gaccgccgta
     1981 tcccacagac gaaacggttt ccgtgaaaac attttcggtt tcagacggca tttcgacatc
     2041 ccgtttcccg acaaccgtcc gactttccgg aatcaggcgc gaaatccgcg cctccgcaga
     2101 aaccgttgcc ttgttcggtt ctttcaaatt gacggaaacg cggcggcagg catcgcggac
     2161 ggctgtgccg tctgaaacgg actgtccttg ggaaacatcc ggggattttg cctccgcctg
     2221 ccctgcatga cggcggaacg gcgcattccg tgcattttcg gctttgactg cagaaaaggc
     2281 agacggatgc tgcttctcaa aatacggctc aaatccgtaa tccgcagact cggaaaacgc
     2341 ttctttgtcc gcatcgaaca tacgggaata cgtgcgttga agcacagggt cgtccaaacc
     2401 gataatctgc agcccttcca tcggggtatc ggacacgcgg actttgggga ccgctgtttc
     2461 gtttttctca aatgcatcga tatagcgttt tttcgtttcc cttaaagccg cgccgtttaa
     2521 ggcacgcgtt gcttcttcca aagtgatttc cttaaaaacc ggacggacgg gcgaaatttc
     2581 gctttcagac ggcatcaacc cttcagcaat actccggtcg aatgggatgc ggcggttgtc
     2641 gattacggca gttgcaatgt cttccgtatc ggcagcctct tcctccgcag cttctgctgc
     2701 ttccgtttcc gcttcttccg tcccgtttcc actgtctgaa tatccgtcgg atgcgttcgg
     2761 aacatccgca cggtttgcag aagcaggcgg aacaggccta tccggttcgg attcgtgcgc
     2821 cagatagttt cggcagaaac ggacgacacc gacgaacagc caatacactg ccgttttgac
     2881 ggcatggaaa agcatcaggg caagttcggg aaaatcgggc ataccgtctt tgatttcagg
     2941 cagctccgcc tgtttttccc ctttcttttc ctgccacgca gaaacctcgc gcatccactc
     3001 gcgttcggat tgtgcgcgga caaaaaacaa gccggcaagc gcaagcaaca aaataacgat
     3061 cataactatc caaaacatat gccgtttccc gcaagacaaa cgattaaacg ggtttatttt
     3121 aacgtgtttc catcccaaaa aaaacaaccg gtcggcaatt ctgccgaaac cgattcggct
     3181 ttcggtttgc ccccaccgac agtataatag ccgtcccttc ccttaatctt tgcggcattg
     3241 ccatgatttt ccaaacagtc tggttttcag atgtggtact gtcggtttcg tggattgtcc
     3301 ttatcctgat actggcagct tccgctccgt cggcattccg ctcgcttgcc cgctaccgtt
     3361 ctgcccttcc cctgtgtacc gtaattttct ccgcggcatg gtgtctgaac gcctctgccg
     3421 gcggcggaca gcttgcacag atgaactacc acctgctcgc cgtcaatcta gttgctctga
     3481 tggtgggcac ttccgccgcc ttttggcttg ccgcgctgct gatgctgccc tactgcctgc
     3541 tgtttgccgg ttctgtcggc gcatatccgc cgaacgcttt ggtgctgatt ctgcccgcgc
     3601 tggccgtcaa ccgcctgtcg cgtatgctgg tcaaccgcct gccacccaat attttcatct
     3661 ttattttcgt caacggcttt ctcgcttccg ccgccggcat tctgctgacc gggctggtgc
     3721 tgaccggtat tttggatgcc gcaaacgctt ttccgtccga aatattgtgg acgaccgccc
     3781 tgcccgtctt tattctgctg gcgtgggcgg aagcttttct cagcggcatt tcaactgcca
     3841 tatttgttgc actgaagccc cattggatca acactttcga cgacaaccgc tatctgaagt
     3901 ccgaccgcgg catatggcgg tagccttcag acggcataga caggcaaaac catgctttcc
     3961 aacatcatcc ccctttccat cggtgcggca ctcggtgcaa cggcgcgttg gctgctcaac
     4021 ctcgccgttc ctgccgccat gtctcccgcc acgggcaacc tgtttgccaa ctggacgggc
     4081 gcgctgctca tcggcatctt tgccgaaacc atcaatcacc cgcaatggaa gctgctgctg
     4141 attaccggtt ttctcggcag cctgaccacg ctttccggat tttcactgga aaccgtaacc
     4201 ctgctccaat cgaatcgtcc tgcttcagca cttgccaata ttttcctaca cacggcaggt
     4261 tcgctgctgc tgacttggct cgggctgaag ataggggcgg cagtcaaata accgcaacaa
     4321 attgcctccc tttgttttga cataatctat aaattagctt cgcttgttta aatactggct
     4381 gtgcca
//
LOCUS       DAWHXZ010000169         4378 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000169 DAWHXZ010000000
VERSION     DAWHXZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.169"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..497
                     /gene="hisF"
                     /locus_tag="WIE35_001689"
     CDS             <1..497
                     /gene="hisF"
                     /locus_tag="WIE35_001689"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ4154576.1"
                     /translation="RLLNAGADKVSINTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAV
                     NSENTRWEIFTHGGRNPTGLDAVEWAIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRA
                     VAEAVDIPVIASGGVGNVRHLIEGITEGKADAVLAAGVFHFGEIAIREAKRAMREAGI
                     EVRL"
     gene            528..923
                     /gene="hisI"
                     /locus_tag="WIE35_001690"
     CDS             528..923
                     /gene="hisI"
                     /locus_tag="WIE35_001690"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ4154577.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGGAWETADAVLKDEKEIYGSTH"
     gene            1030..2625
                     /locus_tag="WIE35_001691"
     CDS             1030..2625
                     /locus_tag="WIE35_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ4154578.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRNTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWTPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGRFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            2644..2831
                     /locus_tag="WIE35_001692"
                     /pseudo
     CDS             2644..2831
                     /locus_tag="WIE35_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
     gene            2850..2966
                     /locus_tag="WIE35_001693"
     CDS             2850..2966
                     /locus_tag="WIE35_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HEZ4154579.1"
                     /translation="MPSEPEKRLSDGIFACNSNADGQNQGASDTVIGCVPSA"
     gene            complement(3000..3530)
                     /locus_tag="WIE35_001694"
     CDS             complement(3000..3530)
                     /locus_tag="WIE35_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ4154580.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(3571..>4378)
                     /locus_tag="WIE35_001695"
     CDS             complement(3571..>4378)
                     /locus_tag="WIE35_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ4154581.1"
                     /translation="RYRTPNIKYIYQDNQGTAEARNTGIKNSNGKYIVFIDSDDFINC
                     QVLLDFLSKDDIDMPDVVFLNAVKYDKGSVSHFGEDYQPEKILKQSKVEVLKGLCRFR
                     KFPGSACNKIIKRELIIKEKLFFEKEVYAEDIEWSMRLFNAATTFSYLDGCYYYYRQG
                     RKDSKTGTVSEKSIKSLLYILEKNAEMEFDRDISSYLYSFLSYEYLVLLFIMTSKNIE
                     CDADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
BASE COUNT         1148 a         1223 c         1036 g          971 t
ORIGIN      
        1 gccgcctgct caatgccggc gcggacaaag tcagcatcaa caccgccgcc gttacccgtc
       61 ccgacctgat tgacgaagcc gccggatttt tcggttcgca agccatcgtc gccgccgtcg
      121 atgccaaagc cgtcaactcc gaaaacacac gctgggaaat ctttacccac ggcgggcgca
      181 atccgaccgg tttggatgcg gtggaatggg cgatcgaaat gcaaaaacgc ggcgcgggcg
      241 aaatcctgct caccgggatg gacagggacg gtacgaaaca gggtttcaac ctgccgctga
      301 cccgcgccgt tgccgaagcc gtcgacatcc ccgtcatcgc ctccggaggg gtcggcaatg
      361 tccggcacct gattgaaggc ataaccgaag gcaaagccga tgccgtactt gccgccggcg
      421 ttttccattt cggggaaatc gccatccgcg aagccaaacg cgctatgcgc gaagccggca
      481 tcgaagtgcg cctctgaccg ccccgaccat gccgtctgaa aggaaatatg gataaaaacc
      541 tgcttgaagc cgtcaaattt gacgaaaaag gtttggtttg cgccatcgcc caagatgccg
      601 aaaccaaacg tgttttaatg gtagcgtgga tgaacgccga agccctgcaa aaaaccgtcg
      661 aaaccggctt tgcccactat tacagccgtt cgcgccaaaa acaatggatg aagggcgaag
      721 agtcgggaca cacgcaaaaa gtccgcgaac tgcgcctcga ctgcgacggc gacgccattg
      781 tgatgctcat cgcccaaaac ggcggcatcg cctgccacac cgggcgagaa agctgctttt
      841 acaaagtctg gcgcggcggc gcgtgggaaa ccgccgatgc cgtcctgaaa gacgaaaaag
      901 agatttacgg cagcacgcac tgaccgcctc caacattgaa ttatcaggca tttttttgta
      961 caatttcgcc gtctcaaaca ctgtccgggc cgtctgaaaa gcggcctgaa cctttttgca
     1021 aagaaaacca tgtcccaaga aatcctcgac caagtgcgcc gccgccgcac gtttgccatc
     1081 atctcccacc ccgacgcggg taaaaccacg ctgaccgaaa agctgttgct gttttcgggc
     1141 gcgattcaga gcgcgggtac ggtaaaaggc aaaaaaaccg gcaaattcgc cacctccgac
     1201 tggatggaca tcgagaagca gcgcggcatt tccgtggcat caagcgtgat gcagttcgac
     1261 tacaaagacc acaccgtcaa cctcttggac acgccgggac accaagactt ctccgaagac
     1321 acctaccgcg ttctgaccgc cgtggacagc gcgttgatgg tcatcgacgc ggcaaaaggt
     1381 gtggaagcgc aaaccatcaa actcttgaac gtctgccgcc tgcgcaatac gccgattgtc
     1441 acgttcatga acaaatacga ccgcgaagtg cgcgattctt tggagttgct ggacgaagtg
     1501 gaaaacatcc tgcaaatccg ctgcgcgccc gtaacctggc cgatcggcat gggcaaaaac
     1561 ttcaaaggcg tgtaccacat cctgaacgac gaaatctatc tctttgaagc cggtggcgaa
     1621 cgtctgccgc acgagttcga catcatcaaa ggcatcgaca atcccgaatt ggaacaacgc
     1681 tttccattgg aaatccagca gttgcgcgac gaaatcgaat tggtgcaggc ggcttccaac
     1741 gagtttaatc tcgacgaatt cctcgccggc gaactcacgc ccgtattctt cggctctgcg
     1801 attaacaact tcggtattca ggaaatcctc aattcattga ttgactggac gcccgcgccg
     1861 aaaccgcgcg acgcgaccgt acgtatggtc gagccggacg agccgaaatt ctccggattt
     1921 atctttaaaa tccaagccaa tatggatccg aaacaccgcg accgcatcgc cttcctgcgc
     1981 gtctgctccg gcagattcga gcgcggtatg aagatgaaac accttcgcat caaccgcgaa
     2041 atcgccgcct ccagcgtggt aaccttcatg tcgcacgacc gcgagctggt ggaagaagcc
     2101 tacgccggcg acatcatcgg catcccgaac cacggcaaca tccaaatcgg cgacagcttc
     2161 tccgaaggcg aacaactggc gttcaccggc atcccattct tcgcgcccga actgttccgc
     2221 agcgtccgca tcaaaaaccc gctgaaaatc aagcaactgc aaaaaggctt gcaacaactc
     2281 ggcgaagaag gtgcggtgca ggtgttcaaa ccgatgagcg gcgcggattt gattttgggc
     2341 gcggtcggcg tgttgcaatt tgaagtcgtt acctcgcggc tggccaacga atacggcgta
     2401 gaagccgtgt tcgacagcgc atccatctgg tcggcgcgct gggtatcgtg cgacgacaag
     2461 aaaaaactgg cggaatttga aaaagccaat gcaggcaacc tcgccatcga cgcgggcggc
     2521 aacctcgcct acctcgcccc taaccgcgtg aatctgggac tcacgcaaga acgctggccg
     2581 gacatcgtgt tccacgaaac gcgcgagcat tcggtcaaac tttaaaaagc aatcggcaat
     2641 aaaatgccgt ctgaacccga aaaaagggct tcagacggca tttttgcctg caactcaaat
     2701 gcaccgatca aaatcaaggc gcatcggata ccgttatcgg gcatcccgtc cgcatgaatt
     2761 tagggctgac gcaagaacgt tggccggaca tcgtgttcca cgaaacgcgc gaacattcgg
     2821 tcaaactgta aaaagcaatc ggcgataaaa tgccgtctga acccgaaaaa aggctttcag
     2881 acggcatttt tgcctgcaac tcaaacgcag acggtcaaaa tcaaggcgca tcggataccg
     2941 ttatcggatg cgtcccatcc gcatgaattt ggggctgacg caagaacgcc gatgtgattt
     3001 cacatcccgt actgtttcga cagcttcaca taatgcgcgg cggaatattt caaaaaggct
     3061 ttttcctcat cggtcagcac gcgcacttgt ctgaccggcg aaccgacata aaggtagccg
     3121 cccgccaaac gtttgcgcgg aggcacgagg ctgcccgcgc cgatcatcac ttcgtcctca
     3181 atcacggcat cgtccaaaac ggtgctgccc ataccgacca ggacgcggtt gccgatacgg
     3241 cagccgtgca gcatcacttt gtgccccacg gtaacgtctt cgccgataac cagcggcgac
     3301 ccttcgggtt tggcggcggt tttgtgggaa acgtgcagga cgctgccgtc ctgtatattg
     3361 ctgcgcgcgc cgacggtgat gctgttcaca tcgccgcgca acacggcgca cggccacaca
     3421 gaaacgtctt cggcaagcga cacttcgccg atgacgacgc acgcttcgtc tatcatacag
     3481 ctttcgtgga tttcgggcgt gcggttttgg aaagttcgga ttgcgttcat ttttcctcct
     3541 tcggtaagat gtatagtgtt aaaggattta ttaaatattc cccctgattg cttttaaaat
     3601 cctgcctgta atatcgaccc cgaataatgt gattatcggg aatatcagct tatatatcaa
     3661 tttattggac tttaacagca taaaccttaa atgatacgcc cttcttttta tatcagcatc
     3721 acactctata tttttactcg tcatgataaa aagcaaaacg agatattcgt aggaaagaaa
     3781 agaataaaga taacttgata tatccctatc aaattccatt tccgcatttt tctccaaaat
     3841 atataataat gactttatac ttttttccga aacagttcct gttttagaat cttttcttcc
     3901 ctgccgataa tagtaataac aaccgtccaa ataagaaaaa gttgttgccg cattaaataa
     3961 tctcattgac cattcgatat cttcggcata aacctctttt tcaaaaaaca gtttttcttt
     4021 gataatcaat tcccttttta taatcttatt acacgccgaa cccggaaatt ttctaaatcg
     4081 gcataatcct ttcaaaactt cgactttgga ttgcttgagt attttttcag gctgataatc
     4141 ttcgccaaaa tgtgaaacac ttcccttatc atatttaacc gcatttaaaa aaaccacatc
     4201 cggcatatca atatcatctt tactaagaaa atccagcaag acctgacaat taataaaatc
     4261 atccgaatca ataaagacta tatattttcc gtttgaattt tttattccgg tattcctagc
     4321 ttctgccgtc ccttggttat cctgatatat atatttgatg tttggtgttc ggtatctg
//
LOCUS       DAWHXZ010000170         4344 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000170 DAWHXZ010000000
VERSION     DAWHXZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.170"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            345..1265
                     /gene="ribF"
                     /locus_tag="WIE35_001696"
     CDS             345..1265
                     /gene="ribF"
                     /locus_tag="WIE35_001696"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ4154582.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMAELKRQIAADMEAAKCW"
     gene            1406..4195
                     /gene="ileS"
                     /locus_tag="WIE35_001697"
     CDS             1406..4195
                     /gene="ileS"
                     /locus_tag="WIE35_001697"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ4154583.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDRPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGISILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETLRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMASYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKASDGEKCERCWHYTHDVGAVAGHETICKR
                     CAGNVGGEGETRHYA"
BASE COUNT         1065 a         1199 c         1153 g          927 t
ORIGIN      
        1 ccttcggcgc ggcaaacgca accacgcgct tttggtttgg aaataatccg attgccgcag
       61 aaatgccgtc attcccgcct gcgcgggaat gacggcggag ggttgtttgt ctcggtttac
      121 ctggttaaaa aagaacgatt ttcactgatg ttgcatcagg tttggggcga tgtttcaaca
      181 cataggatcg cgcctgatgc acgctttatg cgtttggcgc gttcggcggc gggaaatttg
      241 cctgcttttc cagcgtcggg cgggcgtaac gggcggcaca ctgtctataa accgcaatac
      301 cgtttacaat gaccgcctgt ttcaccacat acccgaacgc aacaatgaga atcaggctgg
      361 ggcggcacaa cgcgcccgac tttccgcacg gggccgccgt aaccataggc aatttcgacg
      421 gcgtacacct tggacacaaa cacatcctcc aaaaactccg cctcgaagcc gatacgcgcg
      481 gattacccgt cgtgaccgtc gttttcgaac cccaacccaa agaatttttc gcactccgca
      541 ccggcaggat gccaccgtgt cggatcagcc ccctgcgcac caagctcgaa ttattggaag
      601 gcacaggctg tgtcgatgcc gtctgggttt tgcgtttcga tcaaaatttt tccgaaatat
      661 ccgcgcaagg gtttatcgac cgcctgctgc gtcaaacctt gaatacgcgt tatttgctcg
      721 tcggcgatga tttccgtttc ggtgcggggc gggaaggctg ttttgaactt ttggcacaac
      781 agcccgatat gcagaccgag cgtacgcctt ccgtcatcgt cgaagacatc cgcaccagca
      841 gtaccgccgt gcgacaagcc ctttcagacg gcaaccttgc ctatgcgaaa aaacttttgg
      901 gacacgacta cgtcttgagc ggcagggtgg tgcacggcag aaaactcgga cgcaccttaa
      961 acgccccgac tgccaacatc cgcctgcccc gccaccgtta tgcactcggc ggcgtgttcg
     1021 tcgtcgaagc ggacggcgca ttcggcacgc ggcgcggcgt ggcgagcttc ggtttcaatc
     1081 ccaccgttga tagcggctgt tctcaaaagc ttgaagtcca cctgttcgac tttcaaggcg
     1141 acctgtacgg acaacggctg aacgtccgct tcctgcacaa actgcgcgat gaagaaaagt
     1201 ttgacggtat ggcagaactg aaaaggcaga tagcagccga tatggaagcc gcaaagtgtt
     1261 ggtagaaaaa ccttatacaa accatccgat tgggctacaa tcaatctttt aactgttcag
     1321 acggcatagg ggtttcccgt tgtgaaatac tgtttgaggc gcaatgccgt ctgaaccgaa
     1381 atattgtaac aatagagatt aaaaaatgac cgattacagc aaaaccgtca acctgctcga
     1441 aagcccgttc ccgatgcgcg gcaatcttgc caagcgcgag cctgcatggc tgaaaagctg
     1501 gtacgagcaa aaacgctacc aaaaactgcg cgaaatcgcc aaaggccgtc cgaaattcat
     1561 cctgcacgac ggcccgccgt atgccaacgg cgacatccac atcggtcatg ccgtcaataa
     1621 aattctcaaa gacatcatta tccgcagcaa aacccaagcc ggttttgacg cgccttatgt
     1681 gccgggttgg gactgccacg gcctgcccat cgaagtgatg gtagaaaaac tgcacggcaa
     1741 agacatgccc aaagcgcgtt tccgcgaatt gtgccgcgaa tatgccgccg aacaagttgc
     1801 ccgccagaaa aaagacttta tccgcttggg cgtgttgggc gattgggacc gtccttacct
     1861 gaccatggat ttcaaaaccg aagccgatac cgtgcgtatg ctcggcgaaa tctacaaatc
     1921 cggctatctc taccggggtg cgaaaccggt tcaattctgc ttggactgcg gttcttcgct
     1981 ggccgaagcg gaagtggaat ataaagacaa aatctcgccc gcgattgacg ttgcctatcc
     2041 gtttaaagac accgccgcgc ttgccgccgc attcggtttg gcaggtatcg aaggcaaagc
     2101 gtttgccgtt atttggacaa ccacgccttg gactttgcct gcgagccaag ctgtttcagc
     2161 gggtgcagac gtggtgtatc aattaatcga tacgcccaaa ggcaaattgg tattggccaa
     2221 agatttggcg gaagacgctt tgaaacgcta cggcttttca gacggcattt ctattcttgc
     2281 cgaaaccacc ggcgacaagc tggaaaacct gcacatgaac catccgttcc tcgaacgcga
     2341 tattcccatg cttaacggcg aacacgttac caccgatgcc ggtaccggct tggtgcatac
     2401 tgcgcccgcg cacggtttgg aagactacgc cgtctgcaac aaatacggca tcgagcttta
     2461 caatcctgtc aacgccgaag gcaaatacat cggcgaaacg ctgcgcgttg ccggtatgcg
     2521 cgtttgggag gcgaaccccg tcatcctgca atggttggaa gaaaccggca accttttggc
     2581 aagcagtaaa atcgaacaca gctacgccca ctgctggcgg cacaaaacgc cgctgattta
     2641 ccgcgcgacc ggtcaatggt ttgtcggtat ggacaaagcc ggtgccgacg gcaaaaccct
     2701 gcgcgacaaa gccatcaagg ccgtggacga caccgaattc ttcccgtctt ggggtcgcgc
     2761 gcgtttggaa gccatgattg aaggtcgtcc tgactgggtg gtttcacgcc aacgctattg
     2821 gggcacgccg atgactttct ttgttcacaa agaaacgggc gagctgcatc cgaactctgc
     2881 cgaacttttg gaaaaagttg ccctgaaaat cgaagaaaaa ggcatcgagg cttggttctc
     2941 cctcgataaa agcgaattat taagcgcgga agattgcgaa cattacgaca aactccccga
     3001 tacgatggac gtatggttcg attccggttc gacccattat tccgttgtga aacaacgcga
     3061 agaattggaa tggccggcgg atttgtatct cgaaggcagc gaccagcacc ggggctggtt
     3121 tcaatcgtcc atgctgaccg gctgcgcctc atcaatggga cgcgcaccgt ataaacagct
     3181 gctgacccac ggttttgttg tagacggcga aggcaaaaaa atgtcgaaat ccatcggcaa
     3241 cgtcgtcgcg ccgcaagaag tctataacga attcggcgcg gacatcctgc gtctgtgggc
     3301 ggcatctacc gattacagcg gcgaattggc gatttctaaa gaaatcctta agcgcgtaac
     3361 cgaaagctac cgccgtatcc gcaatacctt gagctttttg tttgccaact tgagcgactt
     3421 taatccgatt gaagatgccg tgcaacaggc ggatatggtg gaaatcgacc gctatgccct
     3481 ggtgttggcg cgtcggctgc aagagcgtct ggcaggcgat tactatccgc gctacgcctt
     3541 ccacttcgcc gtaaaagaca ttgtttcttt ctgctcggaa gacttgggcg cgttctacct
     3601 cgacatcctg aaagaccgcc tctataccac caaagcagac agccatgcac gccgcagcgc
     3661 acaaactgcc ctgtatcaca tcacacgcag tttggttctc ttgattgcac cgattttgtg
     3721 cttcaccggc gaggaagcgt gggacatcat cggcggcggc gaagaagaca gcgtcctttt
     3781 ccatacttgg cacgagttcc cgaccatcaa cgaaaaagcc gaagccgaac tggtgaaaaa
     3841 atggacggca atccgcgaag cccgcgaagc ggtaaccgcc gccattgagc ctttgcgcgc
     3901 cgacaaaacc gttggttcgt ccttgcaggc ggaagccgaa atcactgcgc cggaagaaat
     3961 ggccagctac ctgaacgctt tgggcgaaga attgcgcttt gccctgctgg tgtctaaagc
     4021 cgaagtgaaa gtaggtgatg aacttgccgt tgccgccaaa gccagcgatg gtgaaaaatg
     4081 cgaacgctgt tggcactaca cccacgatgt gggcgcagtt gcaggccatg aaaccatctg
     4141 caaacgctgc gccgggaatg tcggcggaga aggcgaaacg cgccattacg cctgataaag
     4201 tttgagcaaa tgccgtctga aaccgccaaa cagcatttca gacggcattt tttgtaccgc
     4261 gatttgtctt tataacggcg gaggggtttc aagattgcgg tgttgtcgga atgcaactga
     4321 accgtcattc ccacgaaagt ggga
//
LOCUS       DAWHXZ010000171         4339 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000171 DAWHXZ010000000
VERSION     DAWHXZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.171"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            52..1005
                     /locus_tag="WIE35_001698"
     CDS             52..1005
                     /locus_tag="WIE35_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ4154584.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1116..1439)
                     /locus_tag="WIE35_001699"
     CDS             complement(1116..1439)
                     /locus_tag="WIE35_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154585.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1612..2973)
                     /locus_tag="WIE35_001700"
     CDS             complement(1612..2973)
                     /locus_tag="WIE35_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ4154586.1"
                     /translation="MLKKWLNKMLPSSRSSKEAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKEAGFQAYVVGGAIRDLLLGIEPKDFDVATDAMPEQVHKLFRRSRIVGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETTAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3328..>4339)
                     /locus_tag="WIE35_001701"
     CDS             complement(3328..>4339)
                     /locus_tag="WIE35_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ4154587.1"
                     /translation="TVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWR
                     KAQPFDMGFALGPRINAAGRLDDMSVGIACLLARDDAEAQELAAQLNNLNIERREIEQ
                     SMLQDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGE
                     VRGSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAV
                     REMVCEDDLSQTFITDGSLPARDITLEQAQNLAKHVWGQGFAPPSFTDEFHVVRQQPL
                     GAEGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWE
                     AA"
BASE COUNT         1007 a         1260 c         1080 g          992 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgt
      361 accaaacgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt tgaccgtgtc
      661 accaagctga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tttctttatc cgtccaaccg atacatcaat cgaattttct gcatcggatt
     1141 gcctcatctt cgcaaaacat tgccgaagtt tcgcggttgc acgcctgacg cactttctgc
     1201 cgcgccacgc cttcattccg atcgcttgcc tcaaacgtct tctgaaaagg cgtaacgctg
     1261 catacataca tggtggaatt tgaaccgaaa aggtcgattt tgacggtatt gctgccgctt
     1321 tccaatacgg cgacacggtg ttccaactgc gccagacgtg cttccaaatc gccgattctg
     1381 tcggcaaatg caggcatggc ggcacacgcg gtcaaaatgc ccaaaataca ttttttcatc
     1441 aatttttcct tgtttcttga accctgccca cccggacatc ctttttccga gaggcagggt
     1501 tagactttat ttacggggat gaatcccctt acaaatcagg ataatacata gaacggcact
     1561 ttatgcttca tcccatgtgt tgaaacttca ttgctccttg tctgttgacc cttattccca
     1621 atccgttccc acaaccttcg gcttgggttt gcgcctgcga cggcggcgtt ttttcgcctg
     1681 tccttcgttt ttttcgtgtc gggcggcggc ttcgtttttg gtcatctccg accgctgttc
     1741 cgtcgatgcc gtctgaaacg ccgtccacca ctcggcaagg gcgcggtccg cattgccggt
     1801 ttcggcgcgc aagagcagga aatcataggc ggcacggaaa cgcgcctgtg caaacagttt
     1861 gtgcggacgt gcgcctttgc ggttttcaaa ctgcggctgg aacatccaaa tttcgcgcat
     1921 cgtggcggaa aagcgttgcg gcacgcccca accgcgttcg acggtttcgc gcatcgtatt
     1981 gattgcatcg gacagggcgg gcgcgggttt caaaccctgt tgcagattgc ttttccaatg
     2041 gcgttccaac tcgggccaca tcagagccgc caatacaaaa ccgaccgaaa ccgatttgtc
     2101 ggcacgcagc cgctcatcgg tatttttcag ggcaagcacc gtcatttttc cggcgatgcc
     2161 gtctgaaaca cgcaaggcat tgagcagcgg atggatgtcg tccggtatgt caaatccgtt
     2221 caaacgtttc agacactcgc gcgcgtgccc tgaaaacagc agtttcatga tttcgtcaaa
     2281 cagcctcgct accggttcgt gcttcagacg gcatatcgat tcggcaatcg gtgcggtggt
     2341 ttcttccgac agctcaaagc ccaatttgcc cgacaggcgg atggcgcgca aaatcctgac
     2401 agggtcttcc tgatagcgtt cggcggcatc gccaatcata accagcctgc gggcggcaac
     2461 atcggcaatc ccgttgtgga aatccaaaat ctcttctttt tccggatcgt aatacaaggc
     2521 attgcaggta aaatcgcgcc gcatcgcatc ttcttcgatg ctgccgtagg tgttgtcctt
     2581 cataatcctg ccgtgcgcgt tctgatgaac tttcgcaccg ccgcgaaacg tcgttacttc
     2641 gataatctcc gcaccgttca tcacatggac gatttgaaaa cgcctgccga cgatgcggct
     2701 gcggcggaag agtttgtgca cctgttcggg catggcatcg gttgcgacat cgaaatcctt
     2761 gggttcgatg ccgagcagca ggtccctgat cgcgccgccg accacataag cctgaaaccc
     2821 tgcctctttc aggcggcgta tgacgttttc ggcggcaaag ctcaacattt cggcacggat
     2881 gttgtgtctt tcggcaggaa tgaccgtttt actttccgct tctttactgc tccgactgga
     2941 aggcagcatc ttattcaacc attttttcag cataaatatt ctgacccgaa gagcggcatc
     3001 tccacgggca accgtgttca gacggcatcg ggcgcaggtt tgcaagatgc cgtaaccata
     3061 aacaaaaggc gtagaaacta cgccgtccga atcacgccgc cctcgcggcg gcaacgcgtc
     3121 attataacag attgccctcc aaaaaaatca gaccctgctt ttgtggcaga gtctgaaatt
     3181 tgatgcacct tatatattgc gtccctaaga agggatgatt aacaaaaatt aacgcccttt
     3241 actttctttt ccgattggga acattcccgt cgtacaaaca ggcaaatgaa ggaacagaaa
     3301 tcacattcaa acggcgttcc gccgcctcta cgccgcttcc cagtagtcga tataaagctg
     3361 caattcgagg ttgttgcgcc attcgttggc aacggggcgg tacaccgtgc ggatgtattc
     3421 gggaatgtct tcgctgcacc gccagaacat tgcttcaaat tcgcagccgt ctttttgcag
     3481 ccacgctttt ttgtgtttgc cttccgcgcc caaaggctgc tggcggacga cgtggaactc
     3541 gtcggtaaaa ctcggcggcg cgaagccctg cccccaaacg tgtttagcaa ggttttgcgc
     3601 ctgttccagc gtaatgtcgc gtgcaggcag gctgccgtcg gtgatgaagg tttgcgacaa
     3661 atcgtcttcg cacaccattt cgcgcacggc ttcttcaaag gccgtctgaa acgcgggaat
     3721 gttgtgttcg agtatgctca agcccgccgc catcgcgtgt ccgccgaatt tcaaaatcaa
     3781 atcgggatgg cgtttggaca ccaagtccaa agcatcgcgc aagtgaagat tgggaatgga
     3841 acgccccgaa ccgcgcactt caccgttgtc ggcaggcgca aacacgatgg tcggacgata
     3901 aaaacggtct ttgaggcggc tggcaacgat gccgaccacg ccttgatgga agtcgtcgcg
     3961 atacgccacc aaagtcgtct gacctgaagg cagggtttca ggaaaagcat tcagcgcgtc
     4021 ttgcagcata gactgctcga tttcgcgacg ttcgatattg aggttgttta actgcgccgc
     4081 cagttcctgc gcctcggcat catctcgcgc caacagacag gcaataccga ccgacatatc
     4141 gtccagccgt ccggcggcgt tgatgcgcgg ccccaacgca aaacccatat caaacggctg
     4201 cgccttgcgc caatcgcgcc gcgccacttc aaacaaggcg cggataccgg ggcgcatttt
     4261 gccggagcgc atccgtttca aaccttgcga cacgaggatg cggttgttgt ggtcgagagg
     4321 gacgacatcg gcaaccgtg
//
LOCUS       DAWHXZ010000172         4322 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000172 DAWHXZ010000000
VERSION     DAWHXZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.172"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(109..948)
                     /locus_tag="WIE35_001702"
     CDS             complement(109..948)
                     /locus_tag="WIE35_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ4154588.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1233..1308
                     /locus_tag="WIE35_001703"
     tRNA            1233..1308
                     /locus_tag="WIE35_001703"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1266..1268,aa:Arg,seq:ccg)
     gene            complement(1373..2566)
                     /locus_tag="WIE35_001704"
     CDS             complement(1373..2566)
                     /locus_tag="WIE35_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ4154589.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIATVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEAELVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFAYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2587..3675)
                     /gene="trmA"
                     /locus_tag="WIE35_001705"
     CDS             complement(2587..3675)
                     /gene="trmA"
                     /locus_tag="WIE35_001705"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ4154590.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKKLGDAWQQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTRKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEQTLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     AVERAALFDQFPFTPHIESSVLLKKKIL"
     gene            3818..>4322
                     /gene="aroC"
                     /locus_tag="WIE35_001706"
     CDS             3818..>4322
                     /gene="aroC"
                     /locus_tag="WIE35_001706"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ4154591.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFE"
BASE COUNT         1010 a         1160 c         1086 g         1066 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aattcactat aaattcccat aaaaaaacgg
       61 agcagatacc tgccccgttt ttatttaatc cgaaatttta atctaaattt agaattttgc
      121 accggattgg tttgccatat agtcaacagc cgctttgact tcgtcatcgc tcaaacctgc
      181 attgccgcct ttggcaggca tcgcgttaaa gccttcaagg gcgtgtttgt gcaaggtttc
      241 tttgcctttt ttgatacgcg gtgcccaatc gtcttttttg cctatgccgg gaataccggg
      301 aatcgaaccg ccgtggcaca cctgacaggt tgcttcgaag acttttttac cgtcaacgcc
      361 gaccgcaggg gctgccgcac ccttgtcttc tgccttcgct tctgccggag ctgcactatc
      421 ggcaggagca gaagctgttc ctgaagcggc attgtcggca ggcgcagcct catcaggatt
      481 cgggaaagaa ccgccgcttt tgttcgccat gtaagtaatc gcccgtttaa gttcctgatc
      541 ggtcaggtct gccgcaccgc cttttgcagg catggcgtta aagccgttca gcgcgtgttg
      601 gaacaaggta tcgaagcctt gcgcgatacg cggtgcccaa tcgccgttgt gttccagttt
      661 cggagcgttc ggcacattgc tgtccgccgc gtggcattgg atacagattt tgccgaaaat
      721 ctgttcgcct tggcgttcgc cgacggggat gccgtcgccc atcgtcaatt gtccgacagg
      781 ctggatacgg gtctgcgttg ctgcttccgt agtggcatcg acatcgccga acgagccgct
      841 gcccgccagc ttaatcagga aataaaggac tgcaataaca ataacgatac cgctcacaag
      901 ggtaaacagt gcagagcctt gggctttgtt gtcgcggagt tgtttcattt ggtaggcctc
      961 gccgtcaggt taggttgtgc tgtaaattat agtttggtgt gttaaacgca gttaacaata
     1021 ttttgctgga ttatactgaa ttcacagggt ctttccaatc gctatcattg aaaatatgaa
     1081 aaaatttgcc aacggtatct gtataaaaca aacaatcctt tgaaaataat ggtttatcct
     1141 caagaaaact ctccttatgc cgccatacgc cgcctgccgg cgcaagataa cctttgccaa
     1201 tttgcagaat ttacgttaac cttacgtttt acgcacccat agctcagttg gaagagtgtc
     1261 agtttccgaa gctggaggtc acaggttcga tccctgttgg gtgcgccaat tataaagaga
     1321 ccgtctgaaa gataaatatt tttcagacgg tcttttgact tacttcaaac tcttatttca
     1381 agacttccgc aaatgcgcgg gcaacatagt cggtattcga cgtattcagt ccggcgacgc
     1441 acatcctgcc ggaatccagc aggtaaacgg caaattcgtc gcgcagcctg cggacttgtt
     1501 ccacgctcaa tcctgtgtag ccgaacatgc cgcgctgttt gatgaaataa gcgaaatcgc
     1561 gatcggggat ttgcgcagtt aagacatcat aaagtttctg ccgcatcgca cggatgcggt
     1621 cgcgcatcat ataaacctcg ttttgccaca aggcgtaaag ttcggggctg ttcatcacgt
     1681 cggcggcgat atacgcgccg tgcgcgggcg ggctggagta gatacggcgg acagtgaatt
     1741 tgagctgtcc gaataccaat tccgcttctt ccttattcgg gcaaaccacg ctcaggccgc
     1801 cgacgcgctc gccgtagagc gacaggtttt tcgagaagga attgctgacg aacagcggca
     1861 attccatttc caccgctttg cggacggcgt aggcatcgct gtccaaatcg ccgccgaatc
     1921 cttggtaggc aatgtccata aacggaatca gtttgcgcgt tttgatgatg tgcaacactt
     1981 cgtcccattg ttgttccgac atatccacgc cggtcgggtt gtggcagcag ggatggagga
     2041 tcaggacgct gttttcgggc agggtgttga aaaacgcggt catttcgtcg aatttcacgc
     2101 cgacagtggc agggtcgtaa tatgggtaag tgccaacctc gaaacctgca ccttcaaaaa
     2161 tgccgcgatg gttgtcccaa gtcgggtcgc tgacgtaggc gcgcgcttca ggaaaccagc
     2221 ggtgcaggaa atctgctccg actttgagcg cgcccgagcc gcccaaggtt tgcacggtag
     2281 cgatgcgtcc ttgcgcgagc gcagggttgt ctttgccgaa caataaatgc tgcaccgcgc
     2341 tgcggtaagt gtccaagcct tccatcggca ggtagggcga cggcgcaggc gcggcggcgc
     2401 gggcggtttc ggctcggctc acggattcca acacgggcat tttgccttcg tcgtcgaaat
     2461 aaatgcctat gctcaaattg actttttcag ggcgcgggtc gtttttgaag gtttcgacca
     2521 aactcaaaat cggatcgccg ggatagtatt cgatgtgtcg gtacatagtt cttacctctt
     2581 gatttttcaa agtattttct ttttcaacag tacgctgctt tcaatatgcg gcgtaaacgg
     2641 aaattggtcg aacagggcgg cacgttcgac cgcatgggtt tccaccaagg tgtccaaatt
     2701 agcacgcagc gtttcgggat tgcaggaaat gtagatgatg ttgtcaaact gcgacaccag
     2761 cttcaaagtt tgctcatcga tgccggcacg cggcggatcg acgaaaatag tggaaaatgc
     2821 gtaatccgtc aaagcaatac cgccatcctt aaggcgttta aactcacgtt ttctggtata
     2881 ggcttcggta aattcttcag cagacagacg ggcgatttta atgttgccga tgcggttggc
     2941 ttcgatattc cattgcgccg cgctgacgga ggttttggag atttcggttg ccaaaacacg
     3001 ttcaaacttt tcagataaag gaagcgtaaa gttgccgttt ccgcaataca gttcgagcaa
     3061 atccccatcc aatccttctg ctgcaccgca ggcccattca agcattttct gacacacggc
     3121 ggcattcggt tgggtaaaac tgccttcaat ttgccgataa cggaaatccc ggttgccgac
     3181 cttcaaagtt tccgttacat agtcctgttt taagactatt ttctgtcccc tgctccgccc
     3241 aataacggaa atatccaact gttgctgtaa cgcttgcgcc gcctgttgcc atgcgtcgcc
     3301 gagttttttg tggtaaatca tggtaaccag catttccccg ctgagcgtgg acagaaattc
     3361 gacggcatac caacggtttc tcagttcggc agattgggcg gcaacggcaa tcaattcagg
     3421 catcaggcaa ttgactgcct ctgaagcagc atcgaagcgg tcgcaacgga tcaggcttgc
     3481 cccgccagcc ttctgccctt tttcaaacat ggcgtaaaac atttcaccgc cttcgtgcca
     3541 aatacggaac tcggcacgca tacggtaatg tttgtccgga gattcataca cttcccactc
     3601 aggaacatcc aaacctgcaa aaagggtttt aaggtggtct tttttatctt gaagctgctg
     3661 cgtgtagtcg ctcatatcgc tatcctgaaa aatcagaccg gattataagc cgattcactg
     3721 caaaacacgc aaccccttta ccggcatata acccgttttc ctccaacttt tatgctaaaa
     3781 tccgccgtct ccaacatacc gccgacagaa ggaaaatatg gcaggcaaca ctttcggaca
     3841 actcttcacc gtgaccacgt tcggcgaaag ccacggcgca ggtttgggct gcatcatcga
     3901 cggctgcccg cccggcttgg aattaagcga agcggatatc caatttgacc tcgaccggcg
     3961 caaacccggc accagccgcc acgttaccca acgccgcgaa gccgaccaag tcgaaatcct
     4021 ctccggcgta ttcgaaggca aaaccaccgg cacacccatc gccctcttaa tccgcaatac
     4081 cgaccagcgc agcaaagact atggcaacat cgccaccagc ttccgccccg gccacgccga
     4141 ctatacctat tggcacaaat acggcacgcg cgactaccgg ggcggcggca ggagttccgc
     4201 ccgcgaaacc gccgcccgcg ttgctgccgg agccgttgcc aaaaaatggt tgaaagaaaa
     4261 attcggcacg gaaatcaccg cctacgtgac ccaagtcggc gaaaaagaaa tccggtttga
     4321 ag
//
LOCUS       DAWHXZ010000173         4318 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000173 DAWHXZ010000000
VERSION     DAWHXZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.173"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(24..1133)
                     /gene="mltB"
                     /locus_tag="WIE35_001707"
     CDS             complement(24..1133)
                     /gene="mltB"
                     /locus_tag="WIE35_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ4154592.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1042..1293)
                     /locus_tag="WIE35_001708"
     CDS             complement(1042..1293)
                     /locus_tag="WIE35_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154593.1"
                     /translation="MPLDGIFGGGRQISGGAKGNARHSAKPPIAADAAFRAAPLCRFP
                     TYPICSEHIYEKEKNTAAGNLFGGFVCLYGDGGTPAPGK"
     gene            complement(1334..2761)
                     /locus_tag="WIE35_001709"
     CDS             complement(1334..2761)
                     /locus_tag="WIE35_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ4154594.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDAAACLLNDIRKDILDCRYCG"
     gene            2946..>4318
                     /locus_tag="WIE35_001710"
     CDS             2946..>4318
                     /locus_tag="WIE35_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ4154595.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHILPDLAVNRQSDEPLQALKDFQTTFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNP"
BASE COUNT          895 a         1225 c         1192 g         1006 t
ORIGIN      
        1 aatttaatcc actataaaaa acattacaat cccgggccgc caagcgaatt ggcaatgtcc
       61 ctgaccgccg ttacatacat ccgactgtga ttgtactgcc ataccgtata aaaattgttc
      121 aagcccaaat aatattcaaa cacgccgggt gcggtttcca gtttgaacaa aaccgccttt
      181 tcatcatctg caagctcttc gccggggatg atgccgtacg cctttaaatc cgccaccgtc
      241 cgcgtcaggg cggttttttc gccaatgatt gcctgaacat ccgcacccgg cgccaatgtt
      301 gcagacacca gtattttccc gcccgtgcgc caaccgtgct gcttcatata attggcaacc
      361 gatgccgcga catcgccgat gttgccccat atgtcccgat gtccgtcccc gtcataatcc
      421 accgcccatt tccggtagct cgaaggcata aattgcggca tccccattgc gcccgcatag
      481 ctgcctttaa aggcgaaaac atcgccgcct tcttcttttg ccagctttaa aagctcgacc
      541 aattcttttt ggaaaaaccc ggcgcggcgg gggtaatcaa agcctaaggt cgccaatgcg
      601 tccgccacac ggaaactgcc cgtatttttg ccgtaattcg tttcaatccc gataaccgcc
      661 acgataagtt cggcaggcac gccgtatttt tgcgccacat catcgataag cgcgcggttt
      721 tccgcataaa accggcgcgc gccgcgaaat ttcgccttgc ccgaatttcc cgtgcggaac
      781 acataccacg gacgcgatgt ggaggggcgg tgcataatct tgacgatgtc cgccttgtaa
      841 gccgctttgt caaaaaaatc ctgccattcc gcccgggaaa aatccccttt cccgacttca
      901 tcgtccacaa aacggcggac atttgcattg gcggcaaacc cgctgtcgga taccggtacg
      961 gctgccgcgt caaatacggc tgccgcgtca aacgcggggc ggctttcttt tttcatttca
     1021 tccgcgcggg gggcttgggc ttcatttgcc cggggcgggc gtgcctccat cgccgtacag
     1081 gcagacaaag ccgccaaaca aattgccagc ggcagtattt ttctcttttt cataaatatg
     1141 ttccgaacaa atagggtaag tgggaaagcg gcacaagggg gcggcgcgaa atgcggcatc
     1201 cgccgcaatc ggcggctttg cggaatgccg cgcgttgcct tttgcgccgc ccgaaatctg
     1261 cctgccgccg ccgaaaatgc cgtctaaagg cacttcccct ttcagacggc attgcctgcg
     1321 ccgtatttgc ccgctatccg caatatcggc agtccaatat gtctttgcgg atatcgttca
     1381 gcaggcaggc tgccgcgtct tcgtgttccg cctgtacgaa gacaaagagt cgggcgatga
     1441 ttttgccgat aaagactttt tgcagggcgc aggcgtcggt cagcgtgtgt tcctgcagga
     1501 agctgcggat gtcttcgggt aaggtgtaat cacgggcaac ggcggcaaag cgggcatcgg
     1561 tttgcaggcg gtcgagcagg gtttggtttt tgtccaactt catgcctgct tctttttctt
     1621 cggcggtggc gcggacaaac tggtcgtaaa tttcggcata aagcatatcg ttcgggcctt
     1681 caaaaatcgt gaaggggcgg atgtcgatag cgatattgcc ggcggtgtgt ccgcgttcaa
     1741 aacccttcgc gcccaagagt ttttgcaaca tttgcgcggc ggcgtaagtg tattccgtgg
     1801 cgagggtttt gacgatgttc gcctccatca gctgatgggc gacgggggca acaggcgaaa
     1861 cggaatggca gacgtagcgg taaagaatct cggaaacctg atggcggcgc cggatttcgc
     1921 ggcgttcgta atcgacgaat ctgatgtcgt tgcggacgta tcgttccagg ttttcaagga
     1981 tgtattccat aatgccgtgc gtcatgccga tcagttgcag gcggctgcgg ataaagatgt
     2041 tttggaacgc gcgcaaaccg gcagcgtcgc tctgggagag tttcatcacg gcggttgcag
     2101 gcatttcggc atcgatgcgg ttgacggcgt aacggacggc gcgcaagcct tcggatgcga
     2161 gggtttcgca gcggatgtat gttttgggga cgagcagcag gtcgatgact ttggcgagtt
     2221 tgccgttttt gcgttctttg gcggcaacga ggaggaagtc gctttgcgag ttgccctgcc
     2281 agtattttgc ggcgttgacg taaatggttt gtccgtcggt atattcgtag taagactgca
     2341 tttctcgggc aatcgccgcg ccggaggttt cgggttcggt aacgcccaaa ccaccgccct
     2401 cgcctttgaa aaccatgtcc aaaccctgtg cgatttgcgc ttcatcgccg aactcttgca
     2461 gtggctgcaa caccagcgcg ccttcgatgc cggtacgcag cgtaacgggc acgccgtaat
     2521 gccccgcaat ccgcaagact tcctgaattt caaactggct gcccttgcgc ccgccgtgtt
     2581 ttttgtcgag gaagggcaac agcaaacccg cctgcttcaa ggcaagccat ttgtcttcgg
     2641 gcaggtatcg catcaggtcg ataccgtctg aaaaaatgcg gcggaatgcg gattcgatgt
     2701 gctttaaaaa agcagccgtg tccatagttg acggctgcgc gctcggttcg gtgtgtatca
     2761 tcggcttcct ctgtcggttc ccattaatcg gcggccggtc aaaccgcctg ccacagttta
     2821 gagttgattt tctaaacttt accacaaagt gcgccgggca acaatccgcc gacctttcag
     2881 acggcatcgc gccccctccc gtgctaaaat gaccgtttgc atcactgtcc gccgattgcc
     2941 gcactatgac ctaccccatc cccaaacccc gtgaaaaatc ccgttggtcc aatctttcgc
     3001 aaggctcgct gcccttggct ttggcgcgtt atctgccgca taagcggctc aaggtcgtgc
     3061 tgacccaaga tgcggaacag gcgctacgcc ttcagacggc atggcggttt ttccgtccgc
     3121 acgacacggc ggtgttcctg ccggactggg aaacgctgcc ttacgagcgt ttttcgccgc
     3181 atcaggattt ggtgtcggaa cggctgtcgg cgttgtggca gattaagagc ggcgcggcgg
     3241 acgtgttgtt cgtgccggtt gccacggcga tgcagaagct gccgcccgtg ccgtttctgg
     3301 cagggcgcac gttttggctg aaaacagggc agactttgga tataggccgt ctgaaaaccg
     3361 atttggtaga tgcgggctac aaccatgttt cccatgttgt tgcggcgggc gagtttgccg
     3421 tgcgcggcgg cattgtcgat ttgttcccca tgggcagcga aatgccctac cgcatcgatt
     3481 tgtttgacga tgaaatcgac agcatcaaaa cctttgatac ggacacgcag cgcaccatct
     3541 cccccgtttc cgaaatccgc ctgctgccgg cgcacgaatt ccccaccgac agcgaggcgc
     3601 aaaaaatctt ccgcagccgc ttccgcgagg aagtcgacgg taatccgaac gatgccgccg
     3661 tgtacaaagc cgtcagcaac ggccacttcg gtgcgggcgt ggaatattac ctgccgctgt
     3721 tttttgaaaa cgagctggaa acgctgtttg actatatcgg cgaggatgcg ctgtttgtct
     3781 ctttgggcga tgttcacgcc gaggcaaacc gcttttggag cgacgtcaaa tcgcgttacg
     3841 ccatggcgca gggcgatgaa acttatccgc ctttgcttcc acagcatttg tatctctctg
     3901 ccgatgtatt cgcaggccgt ctgaaaaact acggacaagt gctgcccgat gtttccggca
     3961 aggaacacat cctgcccgac cttgccgtca accgccaatc cgacgagccg ttgcaggcat
     4021 tgaaggattt tcagacgacc tttgacgggc ggattttgct gtgcgccgaa agcttgggac
     4081 ggcgcgaaac catgctcggt ttcttgcagc aaaacggttt gaaagccaaa cctgtgtccg
     4141 actggcaggg cttcttgtcg gcgcatgagc cgctgatgat tacagtggcg ccgttggctt
     4201 acgggtttaa actgggcgga ctgcaatcgc cgaaccaaca gcaaactact tccgcctccg
     4261 aaggagaagg cgatgcggtt accgaccaaa ccgaattttc cgcatccgca acaaaccc
//
LOCUS       DAWHXZ010000174         4188 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000174 DAWHXZ010000000
VERSION     DAWHXZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.174"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..676
                     /locus_tag="WIE35_001711"
     CDS             <1..676
                     /locus_tag="WIE35_001711"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ4154596.1"
                     /translation="ALAEKTETLKQRLHRIVGSHRDARFASSLAAEDMVITDLIAGEN
                     LNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRFRPVREDADRYVESKGRFAFYDS
                     VEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSATRTELPFAEYDAGRDIGKYNPI
                     FDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCTRPVKAGEDIRAGRWWWEGRNSK
                     ECGLHK"
     gene            710..922
                     /locus_tag="WIE35_001712"
     CDS             710..922
                     /locus_tag="WIE35_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEZ4154597.1"
                     /translation="MPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRETAVADI
                     SERSATRLDDQASEAAMEKRKKEGYF"
     gene            961..2247
                     /locus_tag="WIE35_001713"
     CDS             961..2247
                     /locus_tag="WIE35_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ4154598.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDAHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDESTYGTVAAGMIL"
     gene            2244..4058
                     /locus_tag="WIE35_001714"
     CDS             2244..4058
                     /locus_tag="WIE35_001714"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ4154599.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLKAAGIQVRLAELKD
                     YKAKNIADERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCQAGKDFDRRFEELGAKRLLERVDADLDFTASANAWTDNIAALLKEEAAKNRATP
                     APQAMPPADLQTVPESRYCKADPFPAALLANQKITARQSDKDVRHIELDLSGSDLSYQ
                     AGDALGVWFDNNPALVGEILDLLGIDPATEIQAGGKMMPVARALSSHFELTQNTPAFV
                     KGYAAFAHYEELDKIIADNAVLQDFVQNTPIVDVLHRFPASLTAEQFIRLLRPLAPRL
                     YSISSAQAEVGDEVHLTVGVVRFEHEGRARTGGASGFLADCVEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFAADFLYQTEWQ
                     QFAKEGFLHKYDFAWSRDQEEKIYVQHKIREQAAELWQWLQEGAYVYVCGDAAKMAKD
                     VEAALLDVIIQAGHLNRDDAEEYLNTLREEKRYQRDVY"
     gene            4085..>4188
                     /locus_tag="WIE35_001715"
     CDS             4085..>4188
                     /locus_tag="WIE35_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit beta"
                     /protein_id="HEZ4154600.1"
                     /translation="MTVQTKTKGLEWQEKPLSNNERLKTESNFLRGTI"
BASE COUNT         1084 a         1203 c         1117 g          784 t
ORIGIN      
        1 cgcccttgcg gaaaaaacgg aaaccctcaa acaacgcctg caccggattg tcggcagcca
       61 ccgggatgca cggtttgcca gcagccttgc ggcagaagat atggtgatta ccgaccttat
      121 cgccggcgaa aacctcaata tcggcatttt taccttggat acggggctgc tccacacgga
      181 aaccctaaac ctgctggaca gactcggacg cgcatatccc catttgcgga taaagcgttt
      241 ccggccggtt cgagaagatg ccgaccgtta tgtggaaagc aaaggcaggt ttgcctttta
      301 cgacagcgtt gaagcgcgcc gcgaatgctg ccgcatccga aaaatcgaac cgctcaaccg
      361 cgccattgca ggcgcggacg cctggcttac cgggcaacgc cgcgaacagt ccgccacacg
      421 cacggaactg ccgtttgccg aatacgatgc cggacgggac atcggcaaat acaacccgat
      481 tttcgactgg tcggaacacg acgtgtgggc atacatcctc gccaacaatg tgccttacaa
      541 cgatttgtac cggcaaggat ttcccagcat aggctgcgac ccctgcaccc gccccgtcaa
      601 ggcgggcgaa gacatccgcg ccgggcggtg gtggtgggaa ggcagaaaca gcaaagaatg
      661 cgggctgcac aaataattca taaatatcag acagaagaaa taaggaataa tgccgtctga
      721 aacctccgaa atccttgatg tccgcttccg caccgtcggc gacatcagct gcacctgccc
      781 ggtagaaagc accgcgtcca cgccgacgga gattatcaga gaaacagcgg ttgccgacat
      841 ttccgaacgc agcgcgaccc ggctggacga tcaggcaagc gaggcggcaa tggaaaaacg
      901 caaaaaagaa ggctatttct aaatttcaaa aaccctttaa gcagatatga ggaagaaaat
      961 atgacggcac aacaccaggc cccgctccgc ttcattaccg ccggcagcgt cgatgacggc
     1021 aaaagcaccc tgatcggacg gctgctctac gacagcaaag ccctgctgtc cgaccaaatc
     1081 aaaaccttgg aatccggcaa aagcaaaggt ttgaaagaaa ccatagactt ctccatcctg
     1141 accgacggac tggaagccga acgcgaacag ggcattacga tcgatgtggc ataccgctat
     1201 ttttccacgg cgaaacggaa attcatcatc gccgacacgc cggggcacga acaatacacg
     1261 cgcaatatgg ttacgggcgc aagcaccgct tcggcggcag tcgtactggt tgacgcatcc
     1321 caactggatt tcggcgcgca gcccttacag cttctgccgc agaccaaacg ccattccgcc
     1381 atcctccgcc aactcaactg cccgcatatt gtggtggcgg tcaacaaaat ggacctgctg
     1441 gattacagcg aagacaaatt caacgccatt gtggaggcat accgccgact tgccgaacag
     1501 ctcggtttga aagacgcgca tttcgtaccg gtgtccgcac tcttgggcga caacattgtt
     1561 taccccggcg gcaatatgcc ttggtataaa ggcgaaccct tattgtccat tttggaaacc
     1621 ctgccggggg cggacgaagt cagccgcacc gccgacgatt tttatttccc cgtgcaactg
     1681 gtcgtccgcc aagatgccga caaggccgat gatttccgag gctatcaggg gcgcatcgaa
     1741 cgcggttcgg tcgccgtcgg gcaaaccgtc cgcatcgaac cgaacgggct gaccgccgaa
     1801 gtgtccgaaa tcatcacgcc gaaaggggaa gtggcgcagg cttttgccgg cgaggcggca
     1861 accctccggc tggaccgcga catcgatgtt tcgcgcggcg acctttttgt cgataaaaat
     1921 tccccgctcg ccccgcaaaa acatctggaa gccacgcttt gctggtttga cgaacgtccg
     1981 ctcaacaccg cgcgcaaata cctgctcaag cacggcacgc aaaccgtgcc ggcaaaagtc
     2041 ggggaaattg aaagcgtttt ggatgtccgc acgctggaac aagaggccgg cgcggaatcc
     2101 ttgaagatga acgacatcgc caaagtccgc atcaaccttc aaaaacccgt tacggcaacg
     2161 ccttatgcgg aaaacaccgc cgccggttcg tttatcctga tagacgaatc gacatacggc
     2221 actgttgcgg caggtatgat tttatgagtg aacacgatat gcagaacaca aatccgccat
     2281 taccgcctct gccgcccgaa atcacgcagc tcctgtcggg gctggacgcg gcacaatggg
     2341 cgtggctgtc cggctatgct tgggcaaaag caggaaacgg ggcatccgca gggctgcccg
     2401 cgcttcagac agcattgccg gcggcagaac ccttttccgt taccgtcctt tccgcctcgc
     2461 aaaccggcaa tgcgaaatcc gttgccgaca aagcggcgga cagcctgaaa gccgccggca
     2521 tacaagtcag gctcgccgaa ctgaaagact acaaggcgaa aaacatcgca gacgaacgcc
     2581 gcctgctgct ggttacgtcc acccagggcg aaggcgaacc gccggaagaa gccgtcgtgc
     2641 tgcacaaact gctgaacggc aaaaaagccc cgaaattgga caaactccaa tttgccgtac
     2701 tgggcttggg cgacagctcc tatccgaatt tctgtcaggc aggtaaagat ttcgaccggc
     2761 gttttgaaga attgggggcg aaacgcctgc tcgaacgcgt tgatgcggat ttggacttta
     2821 ccgcctctgc aaacgcctgg acagacaata tcgccgcact cttaaaagaa gaagccgcaa
     2881 aaaaccgggc aacacccgcg ccacaggcaa tgccccccgc cgaccttcag acggtaccgg
     2941 agagcaggta ctgcaaggca gacccctttc ccgccgccct gctggccaat cagaaaatca
     3001 ccgcccgcca atccgataaa gacgtgcgcc acatcgaact tgatttgagc ggttcggatt
     3061 taagctatca ggcaggcgac gcgctcggcg tttggtttga caacaatccg gcactggtcg
     3121 gggaaatcct agacctgctc ggcatcgatc cggcaacgga aatacaggca ggcggaaaga
     3181 tgatgccggt tgcgcgcgca ctttcatctc atttcgaact cacgcaaaac actccggctt
     3241 tcgtcaaagg ctatgccgcg ttcgcccatt atgaagaact cgataaaatc attgccgata
     3301 acgccgtttt gcaggatttc gtgcaaaaca cgcctattgt cgatgtgctg caccgcttcc
     3361 cggcaagcct gacggcagaa caattcatcc gtttactgcg tccgcttgca ccccgtttgt
     3421 attcgatttc ttcagcacag gcggaagtgg gcgatgaagt gcatttaact gtcggcgtgg
     3481 ttcgttttga acacgaaggc cgcgccagaa cgggcggcgc atccggtttt cttgccgatt
     3541 gcgtcgaaga agacggcacg gtgcgtgtat ttgtggagcg caacgacggc ttcagactgc
     3601 ccgaagacag ccgcaagccg attgtgatga tcggctcggg caccggcgtc gcaccgttcc
     3661 gcgctttcgt ccaacaacgt gccgcagaaa atgcggaagg caaaaactgg ctgattttcg
     3721 gcaatccgca ttttgccgcc gacttcctct atcagaccga atggcagcag tttgccaaag
     3781 agggtttcct gcataaatac gatttcgcct ggtcccgcga tcaggaagaa aaaatctatg
     3841 tgcagcacaa aatccgcgaa caggcggcag agctttggca atggttgcag gagggcgcat
     3901 atgtttatgt gtgcggcgat gcggcaaaaa tggcaaaaga tgtcgaagcc gccttattgg
     3961 atgtgattat tcaagcagga catttaaacc gggacgacgc agaagaatat ttaaatacgc
     4021 tgcgtgaaga aaaacgctat cagcgtgatg tttattgatt aaatgaaatc ggaaggaaca
     4081 caaaatgacc gtacagacca agacaaaagg tttggagtgg caagaaaaac cgctatccaa
     4141 caacgaacgc ctgaaaaccg aaagcaattt tttacgcggc acgatttt
//
LOCUS       DAWHXZ010000175         4187 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000175 DAWHXZ010000000
VERSION     DAWHXZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.175"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(84..1130)
                     /gene="recA"
                     /locus_tag="WIE35_001716"
     CDS             complement(84..1130)
                     /gene="recA"
                     /locus_tag="WIE35_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ4154601.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEVANEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            1384..2148
                     /locus_tag="WIE35_001717"
     CDS             1384..2148
                     /locus_tag="WIE35_001717"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ4154602.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLDQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGAFPCSDDYYFKLL
                     DALIESRLPDIIDIELFAGETAVRRAVADAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLEAKRLAAKPVVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            2168..4183
                     /gene="rep"
                     /locus_tag="WIE35_001718"
     CDS             2168..4183
                     /gene="rep"
                     /locus_tag="WIE35_001718"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ4154603.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRHKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
BASE COUNT          976 a         1154 c         1157 g          900 t
ORIGIN      
        1 caaaactcaa atcaagccgt tcggaggcgg ctcaaaaaaa cggtacttcg cagcagaagt
       61 accgtttatc gggatttcag gttttattct tcggggcgtt cgccgtcggt ttcgtcctgc
      121 gtcccttcgg tgatgtgcat ttctacgccg ttgagggcgc ggatttttgc gtcgatttca
      181 ttggcgactt cgggattttc cttcagccag acgcggacgt tgtctttgcc ctgaccgatt
      241 ttcgcgccgt tgtagctgta ccacgcgccg gatttgttga tgatgtcgtt tttcacgccg
      301 atgtcgatca attcgccttc ccaactgatg ccttctccgt agaggatgtc aaactctgcc
      361 tgacggaacg ggggggcgac tttgtttttg atgactttga cgcgggtttc gttgcccaat
      421 acttcttcgc cttttttgat ggatccggtg cggcggatgt cgaggcggac ggaagaatag
      481 aatttcagcg cgttgccgcc ggtggtggtc tcggggctgc cgaacattac gccgatcttc
      541 atccggattt ggttgatgaa cacaaccagc gtgttggttt ttttgatgtg tccggtcagt
      601 ttgcgcaaag cctgactcat caggcgcgcc tgcagtccga catggctgtc ccccatatcg
      661 ccttcgattt cggctttggg gacgagtgcg gctacggaat cgacgactac catatctatg
      721 ccgcccgaac ggacgagtgt gtcgcagatt tccaaagcct gttcgccggt atcgggctgg
      781 gacaggtaaa gctcttcgac ttttacgccg agtttgcggg cgtaaacggg atcaaaggcg
      841 tgttcggcat cgacaaaggc gcacacgccg ccgtttttct ggcattgggc gacggcttcg
      901 aggcagaggg tggttttgcc ggaggattcg gggccgaaga tttcgacgat gcgcccgcgc
      961 ggcagaccgc cgactccgag ggcgaggtct aatccgagcg atccggtgga aatgacttcg
     1021 aggttttctt cctgctggct gccgtccatt ttcatgatgg cgcctttgcc gaaacttttt
     1081 tcgatttgcg ccagtgcggc ggcaagggct ttgcttttgt cgtctgacat tggggttact
     1141 ccggaacaaa tgcggtatgt aggatgcggc gcaacacggg ctgcggcgcg ggatgtgtat
     1201 cgttttcccg atgtgcgggc tatcggtaat gctgcttcac gaggttgcca ttatcgcata
     1261 tttccttgct tgccgatatg cggcaggacg cggcggcttg tgccggaatg gaatctggat
     1321 gccgtctaaa aggcggccgg ctttgttata atggcggctg ttttttctgt gtgtgcctgt
     1381 tttatgtgtt cctgccttgt tgtcaaaaat accgttatcg gaagcggacg caccaaaatc
     1441 gccgtgccgc ttgtcgcccg cgatgccgcc gaactttccg ccgtacttga ccaaatcaaa
     1501 aacctgccct tcgacatcgt cgagttccgc gccgattttt tggaatgcgc gggcagcatc
     1561 ggcgaagtat tgcgccacac gcagaccgtc cgcgacgcgc tgcccgacaa gccgctgctg
     1621 tttacgttca gacggcatgg cgaaggcggc gcgttcccgt gttcggacga ttattatttc
     1681 aaactgctcg acgcgctgat cgaaagccgc ctgcccgaca tcatcgacat cgagctgttt
     1741 gccggcgaaa ccgccgtccg gcgcgccgtg gcggatgctc aaaaaaacgg catcgccgcc
     1801 ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag aagaaatcgt atgccgtctg
     1861 aaacagatgg aggactgcgg cgcggacatc tgcaaaattg cggtgatgcc gcaaagcgcg
     1921 gaagatgtgc tgactttgct ttccgccacg ctcgaagcga aacggcttgc cgccaaaccg
     1981 gttgttacga tgtcgatggg gcagacgggt gcggtcagcc gccttgccgg acaggtgttc
     2041 ggctcaagca tcacgttcgg ttcgggaacg caaaactccg cgccggggca aatcggcgta
     2101 tccgccctcc gtgcggcact cgactgtctc gaaagcggcg cagactgatt tcagacggca
     2161 tcaaaacatg atgaaactca atccccaaca gctcgaagcc gtccgttacc tcggcggtcc
     2221 cctgctcgtc ctcgccggcg ctggcagcgg caaaaccggc gtgattacgc aaaaaatcaa
     2281 gtatttgatt gtcaatgtcg gctatctgcc gcataccgtt gccgcgatta cctttaccaa
     2341 taaagccgcc gcggaaatgc aggagcgcgt cgccaaaatg ctgcccaagt cgcaaacgcg
     2401 cgggctgacg atttgcacgt tccattcttt gggcatgaag attttgcgcg aagaggcgaa
     2461 ccatatcggt tacaaaaaaa acttctccat tctcgattct accgacagcg cgaaaatcat
     2521 cggcgaactc ttaggcggta cgggcaaaga agccgtattc aaggcgcagc accagatttc
     2581 cttgtggaaa aacgatttaa aaacgcctga agatgtcgtt cagacggcat cgaacatttg
     2641 ggaacaacaa accgcacgcg tgtatgcgag ctatcaggaa accttacaaa gctatcaggc
     2701 agtggacttt gacgacttaa tccgcctgcc tgccgtgctg ttgcagcaaa acagcgaagt
     2761 gcgccacaaa tggcagcggc ggctgcgtta tctgctggtt gacgaatgcc aagatacgaa
     2821 tacctgccaa tttacgttga tgaaacttct gaccggcgcg gaaggtatgt ttaccgccgt
     2881 cggcgacgac gaccagtcca tctacgcatg gcgcggtgcg aacatggaaa acctgcgtaa
     2941 aatgcaggaa aactatccgc agatgaaggt catcaaactg gagcaaaact accgctccac
     3001 cgcgcggatt ctcaaaatcg ccaacaaagt catcgaaaac aaccccaagc tgtttaccaa
     3061 aaaactttgg tcgcaattgg gcgaaggcga gccggtcaaa gtcgttgcct gccaaaacga
     3121 gcaacacgaa gccgactggg tcgtcagcca aatcgtcaaa caaaaactca tcggcggcga
     3181 caaaacccaa tatgccgatt tcgccgtgtt gtaccgggga aagcatcagg cgaggatttt
     3241 cgaggaagca ttgcgcggcg cgcgcatccc ctaccgtctc tccggcggac aaagcttttt
     3301 cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg ctgcttgcca accccaacga
     3361 cgatcccgcc tttctgcgtg ccgttaccac gcccaaacgc ggcatcggcg atgtaacgct
     3421 gggcaagctc aacacttacg cgcacgaaca cgaatgcagc ctgtatgaag ccgcgcaaaa
     3481 cgaagaagcc ctcgccacgc tgaacaatac caaccgccaa cacctgcaag cctttatgga
     3541 tatgttcggc agttaccgtg ccaaagccga aaccggcgaa gcgggcgagt tcatcaacag
     3601 cctgctcgaa gaaatcgact atgaaaacca cctgcttgcc agtgaagaag gcaaagctgg
     3661 cgaaatcaaa tggcgcaacg tcggcgattt ggtatcatgg tttgcgcgaa aaggcgggga
     3721 agacggcaaa aacatcatcg aactcgccca aaccgtcgcc ttgatgacgc ttttggaagg
     3781 aaaagacgaa gaagaaaccg atgccgtctc gctatccacg ctacacgccg ccaaaggttt
     3841 ggagtatccg tatgttttcc ttgtcggttg cgaagaaggc gttttgccgc acaacgacag
     3901 catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg tacgtcggca tcacccgcgc
     3961 caaacgccaa ctcacactga cccactgcgt caaacgcaaa aaacaaggca catggcagtt
     4021 ccccgaaccc agccgattca tagacgaaat gccgcaggaa gatttgaaaa tcctggggcg
     4081 caaaggcggc gaaccgattg tcagcaagga agaaggcagg cgcaaccttg ccgatataat
     4141 cggaaggctc gacaacctaa aaaaaagcgg cgcggcggat taaaccg
//
LOCUS       DAWHXZ010000176         4011 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000176 DAWHXZ010000000
VERSION     DAWHXZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.176"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..245)
                     /locus_tag="WIE35_001719"
     CDS             complement(<1..245)
                     /locus_tag="WIE35_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ4154604.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVA"
     gene            505..1914
                     /gene="leuC"
                     /locus_tag="WIE35_001720"
     CDS             505..1914
                     /gene="leuC"
                     /locus_tag="WIE35_001720"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ4154605.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            2011..2265
                     /locus_tag="WIE35_001721"
     CDS             2011..2265
                     /locus_tag="WIE35_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154606.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            2327..2968
                     /gene="leuD"
                     /locus_tag="WIE35_001722"
     CDS             2327..2968
                     /gene="leuD"
                     /locus_tag="WIE35_001722"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ4154607.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAGQTLT
                     TPGGETFAFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            3137..>4011
                     /gene="dcm"
                     /locus_tag="WIE35_001723"
     CDS             3137..>4011
                     /gene="dcm"
                     /locus_tag="WIE35_001723"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ4154608.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFY"
BASE COUNT         1144 a         1065 c          942 g          860 t
ORIGIN      
        1 gcaaccgccg ccagcgggat aaagttggga ttcagattat tgaagccgcc ggggaacaaa
       61 ttcatatcga tagacgaaat tttgtaaccg gcattgcgta tatcgaccga accgtaaaac
      121 ggcgggcggt gtacgctcca ctgcgtgcga aaccacgcct cgattttggc gtgattggac
      181 aggatttttg cctcaaacgc ctgaagttgc gccgaatgtt cgggcgacat caccggtaat
      241 ttcatctttc gtctgcctct gtgtgttttt tgacaagatg cagatgataa tgcctttgaa
      301 agaatgacga caagtattct ttcgtatcga atcatgcttt agatataact gtttactgaa
      361 atggacaata cgtctaattt tttcgttaaa attgtttgca aaataacacc ggctcaaaaa
      421 ttagacaaaa tttgttgcgc ggtataaaga atacgtctaa aatccgccca atccgcatta
      481 ccatttatcc aaaagaacaa catcatgaca gcacaaaccc tctacgacaa actttggaac
      541 agccacgtcg tccgcgaaga agaagacggc accgtcctgc tctacatcga ccgccatttg
      601 gtgcacgaag ttaccagccc tcaggcattt gaaggcttga aaatggcggg gcgcaagctg
      661 tggcgcatcg acagcgtcgt ctccaccgcc gaccacaaca ccccgaccgg cgattgggac
      721 aaaggcatcc aagacccgat ttccaagctg caagtcgata ctttggacaa aaacattaaa
      781 gagtttggcg cactcgccta ttttccgttt atggacaaag gtcagggcat cgtacacgtt
      841 atgggccccg aacaaggcgc gaccctgccc ggtatgaccg tcgtctgcgg cgactcgcac
      901 acttccaccc acggcgcatt cggcgcactg gcgcacggca tcggcacttc cgaagtcgag
      961 cacacgatgg cgacccagtg cattaccgcg aaaaaatcca aatccatgct gattgccgtt
     1021 gacggcaaat taaaagcggg cgttaccgcc aaagacgtgg cactttacat catcgggcaa
     1081 atcggcacgg cgggcggtac aggctacgcc gtcgagtttg gcggcgaagc catccgcagc
     1141 ctttctatgg aaggccgcat gactttatgc aatatggcga tcgaagccgg tgcgcgctca
     1201 ggcatggttg ccgtcgacca aaccaccatc gactatgtaa aagataaacc cttcgcaccc
     1261 gaaggcgaag cgtgggacaa agccgtcgag tactggcgca cgctggtgtc tgacgaaggt
     1321 gcggtattcg acaaagaata ccgtttcaac gccgaagaca tcgaaccgca agtcacttgg
     1381 ggtacctcgc ctgaaatggt tttagacatc agcagcaaag tgccgaatcc tgccgaagaa
     1441 accgatccgg tcaaacgcag cggtatggaa cgcgcccttg aatacatggg cttggaagcc
     1501 ggtacgccat taaacgaaat ccccgtcgac atcgtattca tcggctcttg caccaacagc
     1561 cgcatcgaag acttgcgcga agccgccgcc atcgccaaag accgcaaaaa agccgccaac
     1621 gtacagcgcg tgttaatcgt ccccggctcc ggtttggtta aagaacaagc cgaaaaagaa
     1681 ggcttggaca aaattttcat tgaagccggt tttgaatggc gcgaaccggg ctgttcaatg
     1741 tgtctcgcca tgaacgccga ccgcctcgca ccggggcaac gctgcgcctc cacctccaac
     1801 cgcaattttg aaggccgtca aggcaacggc ggacgtaccc acctcgtcag ccctgccatg
     1861 gcagccgcag ccgcagtgac cggtcgcttt accgacatcc gcatgatggc gtaagtattt
     1921 tcaggcggtc tttaagaatt tgacgcggcc gtctgaaata tgtcccgtaa ggcagaaaca
     1981 cctttaatat tcaaaccaag gagtttaacc atgaacaaac ttttcattac cgccctgtcc
     2041 gccctcgcct tgtccgcctg cgccggcact tgggagggcg cgaaacaaga caccgcccgc
     2101 aaccttgaca aaacacaggc cgccgccgaa cgcgccgccg aacaaacagg caacgccgtc
     2161 gaaaaaggct gggacaaaac caaagaagcc gtcaaaaaag gcggcaatgc cgtcggacgc
     2221 ggcatttccc atctcggcgg aaaaatcgaa aacgccaccg aataaccgat tcgcgatgcc
     2281 gtctgaaacc gcttcagacg gcatcgtcag cacaagacgc aaacccatga aagcctttac
     2341 caaaattacc gccatcgtcg ccccgctcga ccgcagcaac gtcgataccg acgccatcat
     2401 ccccaaacaa tttttaaaat ccatcaaacg cagcggcttc ggccccaatg cctttgacga
     2461 atggcgttac ctcgaccacg gcgaaccggg catggacaac agcaaacgcc cgttgaaccc
     2521 tgatttctcg ctgaaccagc cgcgttacca aggcgcgcaa atcctgttga cgcgtaaaaa
     2581 cttcggttgc ggctcttcac gcgaacacgc cccttgggca ttggacgact acggcttccg
     2641 cgccgtcatc gcccccagct tcgccgacat cttctttaac aactgctaca aaaacggcct
     2701 tttgcctatc gtgttgaccg aagaacaggt tgaccggctt ttcaaagaag ttgaagccaa
     2761 cgaaggctac cggctctcca tcgaccttgc cgggcaaacc ctgaccaccc ccggcggcga
     2821 aacattcgca ttcgacatta ccgaacaccg caaacactgc ctcttaaacg gcttggacga
     2881 aatcggactg accctgcaac acgccgatga aattcacgcc tttgaagaaa aacgccgcca
     2941 aagccagcct tggctgttta acggttaaac ctatgccgtc tgaaggccgt tcagacggca
     3001 ttcctgataa catataattg ctgcaactaa tgtaaacaga ggggctataa attgctttca
     3061 tctttcaagc gggcttagca acgctctaaa ttaatgcaat acagctctat ttcaaataaa
     3121 tttttagatt tttaatatgc aacagattaa atttattgac ttattttccg ggatgagcgg
     3181 tatcaggaaa ggatttgaac aagcctgtcg gaaacagtcg gttgcttgcg aatgtgtttt
     3241 tacctcagaa atcaaaccgg cagctttgga agtattgaag caaaactacc ctgatgaagt
     3301 gccgtatgga gatataacga aaattgaaac gggagatatt cccgattttg acatcttgtt
     3361 ggcaggcttc ccttgccagg ctttttcttt cgccggaaaa agattgggct ttgaagatac
     3421 gcggggaacg cttttctttg atgtggcaag gattttaaag gcaaaaaaac caaaaggttt
     3481 tattttggaa aatgtggaag gattggtgac gcacgataga aaagatccga cacaaaaaat
     3541 aggacgcacc ctgaccgtta ttttggaaac cttggaaaca ttgggctact atgtttcttg
     3601 gaaagtttta aatgcaaaag atttcggcat tccccaaaac aggaagcgca tttatctgac
     3661 aggcagtttg aaatccaaac ccgacttgtc ttttgaaaca accctaagtc cgaaattaaa
     3721 aaatattttg gaatcgggac tgcctaccga aagcagtcct ttcatcaaaa aattgctaaa
     3781 gaaatttccc ccgtccgaac tgtacggaaa atcagtaaag gacaaacggg gagggaaaaa
     3841 caatatccac agttgggata ttgaattaaa aggcacagta accgaagaag agaagcaatt
     3901 attaaatatg cttctaaaag aacggaggaa gaaaaaatgg gcttcagaaa tcggcatcga
     3961 ttggatggat gggatgcctt tgacaaaagc gcaaatttca actttctata a
//
LOCUS       DAWHXZ010000177         4000 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000177 DAWHXZ010000000
VERSION     DAWHXZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.177"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(64..1314)
                     /locus_tag="WIE35_001724"
     CDS             complement(64..1314)
                     /locus_tag="WIE35_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ4154609.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSAIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLENVRKQITALCDKYTVYGA"
     gene            complement(1381..1576)
                     /locus_tag="WIE35_001725"
                     /pseudo
     CDS             complement(1381..1576)
                     /locus_tag="WIE35_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1795..3570)
                     /gene="ggt"
                     /locus_tag="WIE35_001726"
     CDS             complement(1795..3570)
                     /gene="ggt"
                     /locus_tag="WIE35_001726"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ4154610.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLIEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGNYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3738..4000)
                     /locus_tag="WIE35_001727"
                     /pseudo
     CDS             complement(3738..4000)
                     /locus_tag="WIE35_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin, Grx4 family"
BASE COUNT          997 a          951 c          943 g         1109 t
ORIGIN      
        1 gcctgaaaca ttgtgagatt tcaggcggca ttggattgct tggcggaata tttttaaaaa
       61 ggcttacgcg ccgtaaacgg tgtatttgtc gcagagggcg gtaatctgtt tgcggacgtt
      121 ttccagattc gcttcgtctt cggggttaga caatacgtcg gcaaccaagt tcgccaatac
      181 gcgcgcgtcg gcttcgttaa aaccgcgtgt ggtcatggca gcggagccga tgcggatgcc
      241 ggaggtaacg aagggttttt ccggatcgtt cgggatggcg tttttgttga cggtgatgtg
      301 cgctttgccc aaagcggctt cggcggcttt accggtgatt ttcatcggtt gcaggtcgac
      361 gaggaaaacg tggctttcgg tgcggccgga aacgatgcgc aaaccgcgtt taaccaactc
      421 ttctgccatg gcggcagcat tgattttcac ttgttttgcg tattgtttga actcgggttg
      481 caacgcttct ttaaacgcta cggctttggc ggcgatgacg tgcatcagtg gaccgccttg
      541 taggcttggg aagatggcgg agtttaacgc tttttcgtgg gtattgtcgc ggcacaaaat
      601 cacgccgccg cgagggccgc gtagggtttt gtgggtggtg gtggtcacga agtcgcagaa
      661 tggcacgggg ttgggatatt cgccaccggc aatcagtccg gcatagtgcg ccatgtcgac
      721 aaagaggtat gcgccgactt tatcggcgat ctcgcggaat tttgcccagt cgatttgcaa
      781 cgcgtaggca gacgcaccgg ccacaatcat tttaggtttg tgttcgagag ccaagcgttc
      841 cacttcggca taatcgagca cttcgttttc atccaaacca taagtaacgg cattgtagag
      901 tttgccggaa atattaacgc tcgcgccgtg ggtcaggtgg ccgccgtgcg ccaaagacat
      961 acccaaaatg gtgtcgcccg gttttaaaac ggaagcgtac acggcttggt tggcttgcga
     1021 gccggagtgc ggttgaacgt tggcgtattg cgcgccgaac agttttttga cgcggtcgat
     1081 tgccaattgt tcgacaatat cgacgtattc gcagccgccg tagtagcgtt tgccggggta
     1141 gccttcggcg tatttgttgg tcagctggga accttgcgct tccattacgg cgcagctgac
     1201 gtagttttca gaggcaatca gctcgacgtg gtcttgctgg cgttggtctt cttgggcaat
     1261 ggctgctgcc aaatcggggt catattgtgc gagggtaacg ctttttgaaa acatgttctc
     1321 ggctcctttg tgtaatcagg gtatcatgag tgttttttgt ataaaaaaat acttcaaaac
     1381 ctaaggcaga tagtccataa tgcgtaaatt ttctttggca ttatcaggta atttatttaa
     1441 catgctggtt tttagcgtct cattaccttt atgttagtac aaggctaccc aaaggcaata
     1501 catcaaatgg taaattttcg gcagtttgtg caaaatcaat caaataacca gcaccaacca
     1561 catctttatc agccataacc cgctgataca ccacttgcca agacaactct cctgccccac
     1621 catgtcttaa aatcagatga atggcttgta atgctgtgtt tggcgtctca caaattgcga
     1681 agatctgctc agaagcttgc ttgatggctt gtatttttgt ttgattactc acaatttccc
     1741 cctattttaa tattaactta atgcggtcaa attcacaaaa tacaagcttc acctctaatc
     1801 acccatcact cgaccttctc ggcgtggatc ggcaccacca accagcctgc ttggctcgat
     1861 aataatggct tgaacacctg aatttagctc acgcacgtca gtcttatagc ccaaatcatt
     1921 taatgcttgt tgccactgga gggcggttgt acccgtttct agttcatagt taccaaagcg
     1981 atttaataaa ttgggtgcac tgatggcatc ttggatatcc atattccagt cactatgtgc
     2041 cacaatcgtc ttagcgacat agccaatgat acggctacca cctggggagc cgattgccat
     2101 ataaggcttg cctgctttaa atacgatggt tggtgccatt gaggagcgtg gtctcttgcc
     2161 gggctcgaca cgattggcga cctgtttgcc ctgctttatt ggctcaaaac taaagtctgt
     2221 cagctcatta ttcagcaggt agccatttgc catcaaagtt gagccaaacg cattttcaat
     2281 ggaagtcgtc attgatagca cattgcccgc cttatccaca attgatatat gactggtaga
     2341 aggtaactca atcgcttgtg aggacaccca ctcatgaata aaatcgcctg cagatacgct
     2401 aggcaatgcc ttatccgact gctcaagcag ctggctgcga tgttttaggt agtctttaga
     2461 aatcaactgg cgaatgggta ctggtacaaa atcagggtcg cccaaatata catcacgatc
     2521 cgcaaacgca agcctagaag cgtcgcccaa gagacgtaaa ccttcagcat cataccccac
     2581 ctgattgggt gaaaattcat ttaaaatccc caaaatttga cccacagcaa tcccacctga
     2641 gcttggtgca cccataccgc atacttcata aatacgataa gtcacacaaa caggcgggcg
     2701 ttccaccact tgataatcag ataaatcttg taaggataat tgaccggggt tatccttagc
     2761 attttggaca actgaaacga tattttgggc atatttacca gtatgcagag cttttgcacc
     2821 ttgagctgct aacgcctgaa cactgtcagc aaattctaaa tttttcagca agctgcctgc
     2881 ttgtagcggc acaccattcg gcaaaaaata agcggctgtt tttggatagc gtgccaaatg
     2941 ctgctgattt tgctcaaccg agatggcaag ccttggcgac acctcaaagc cttgttttgc
     3001 caagcggatc ggtgtatcaa ataattttcc ccaaggcaat acaccgtatc gctgatgtat
     3061 tgtctccatc agtttaggga tagcaggcgt acccaccgag cgaccaccga ccaccgcttc
     3121 cataaatttc aatggttgac catctttatc caaaaataat tccggcgtcg cacgcatcgg
     3181 tgccgtctca cgcccatcaa atgtggtcaa tgttttggcg gtattatccc aatacaacac
     3241 aaatgcacca ccgcccaagc ctgacgactg tggctctacc aagcttagtg tcgtctgcac
     3301 cgccaccatc gcatctgcag cgctaccgcc ttgctttaag atatcatagc cagcttgtgt
     3361 tgctaatgga ttggctgacg ctaccataaa atcacttgca atcacctgct tttgttcgat
     3421 cagtcccgtt gcatgttcag gcgtgtgagc gtctgcacct gtgatgacag cagaatgagt
     3481 attaacctta ccttgattgg catggatgac ttgacatcca gagattgtca tagacattat
     3541 caatgcagtc aataaatatg ttttagccac aagcactcct tcgcctgagt ttgattgatg
     3601 attcatacaa ggcatgctga ttattgtatt taatatggct aaataattca atccaaacta
     3661 tcaatcttga ccatcaaaaa aagaccgcta atgtcatcag cagtcttttt tgatatttat
     3721 tttaagatat taagtaatca gacctttggg ctatgctctt caatgagtgg ttttagctca
     3781 cctgattggt acatttgtag gataatatca ctaccaccga ttaactcacc attaacccaa
     3841 agctgtggaa aggttggccg attggcgatg agtggtagag tactgcgaat ttctgggttt
     3901 tctaggatat taacaaaagc aaagggtctg ccaattgggt cagcacctct actgcacgcg
     3961 ctgaaaatcc acattgggga aactggggcg tgcctttcat
//
LOCUS       DAWHXZ010000178         3930 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000178 DAWHXZ010000000
VERSION     DAWHXZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.178"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..331)
                     /locus_tag="WIE35_001728"
     CDS             complement(<1..331)
                     /locus_tag="WIE35_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ4154611.1"
                     /translation="MFSLYREDSLISRLNRDGYLTSPSADFLELLSLCRDIHALTDGA
                     FDPTVQALWKLYADYFAARPDAETPPPQNSVKEALKRVGFGKVSFDDREIRFAEKGMG
                     LSLNGIAQ"
     gene            complement(312..593)
                     /locus_tag="WIE35_001729"
     CDS             complement(312..593)
                     /locus_tag="WIE35_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
                     /protein_id="HEZ4154612.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNKDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(602..1396)
                     /locus_tag="WIE35_001730"
     CDS             complement(602..1396)
                     /locus_tag="WIE35_001730"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ4154613.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIYELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1780..3114
                     /gene="gdhA"
                     /locus_tag="WIE35_001731"
     CDS             1780..3114
                     /gene="gdhA"
                     /locus_tag="WIE35_001731"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ4154614.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3256..3630
                     /locus_tag="WIE35_001732"
     CDS             3256..3630
                     /locus_tag="WIE35_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154615.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3674..>3930
                     /locus_tag="WIE35_001733"
     CDS             3674..>3930
                     /locus_tag="WIE35_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ4154616.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
BASE COUNT         1070 a         1014 c          925 g          921 t
ORIGIN      
        1 cttgcgcgat gccgttcaag gacaaaccca tacccttttc ggcaaaacgg atttcacggt
       61 cgtcaaacga gactttgccg aagccgaccc gtttcagggc ttccttcacg ctgttttgag
      121 gaggcggcgt ttccgcatcg ggacgggcgg caaaataatc ggcatacagt ttccacaacg
      181 cctgcactgt cggatcgaac gcgccgtccg tcagcgcgtg aatatcgcgg cacaggctca
      241 acagttccaa aaaatccgcc gacggcgaag tcagataacc gtccctgttc aggcggctga
      301 tcaggctgtc ttcacggtaa aggctgaaca attttttcca aacgcgccac ttccgccaaa
      361 accttgttga ccaaatccgc cgcctgtctg tcgtccacac cgaacaggcg cagctccgca
      421 ccggaaccca gcgcgacacc tttccagaag aacacatttt cattgcgttt ttcatcttta
      481 ttgcgttttt catcatcggc ggcaaaagga ttcggcagga aagaaaccgc cgcgcccgcc
      541 gccgcaacgg cggcaactgt caaaaaacgc ctgcgcccga aatgcctgcc cataccgcct
      601 cctaaaccga cactgccgcc ttgatgtgcg gatgggggtc gtaaccttcc aactcgaaat
      661 cttcaaactt gaaggaaaac aaatctttga cttcaggatt gattttcatc actggcaagg
      721 cgcgcggttc gcgttccaac tgcaatgcgg cctgctcgaa atggttgcgg tacaaatgcg
      781 cgtcgccaaa cgtatggaca aactcgcccg cctccaatcc gcacacttgc gccatcatca
      841 tggtcaacaa tgcgtagctg gcaatattaa acggcacacc aaggaaaata tctgcactac
      901 gctggtaaag ctggcaggac agtttgccgt cggcaacgta aaactgaaac agcgcgtggc
      961 agggcggcaa ggccatttcg tcgaccaaag ccggattcca cgccgataca atcaggcggc
     1021 gcgagtcggg gtttttctta atttgttcca ccacattggc gatttggtcg atatgcctgc
     1081 cgtcgggcgc gggccagtta cgccactggt agccgtaaac cgggcctaag tcgccgtttt
     1141 cgtccgccca ctcgtcccaa atggaaacat tgttgtcctt taggtatttg atattggtat
     1201 cgcctttgag aaaccaaagc agctcgtaga taatcgaacg cagatgcagc tttttggtcg
     1261 tcagcagcgg aaaacctttg cccaagtcaa aacgcatctg ataaccgaat acggagcgcg
     1321 tgcccgtacc ggtgcggtct gatttgtccg taccgttgtc gaggacgtgg cgcatcaagt
     1381 ccaaataggc tttcatagca gtctttcatc aaattaaacg gcgcatattg taacatttcc
     1441 ggataatgcc caaaacacgg atacagacag gcaggattgt tggcaatttc agtccttttc
     1501 cacagtaaaa cccggtggga aaacaaaatt accttgattg gaatcaaaaa atctagttta
     1561 attacttaga ataaaatttc aataatattt ttatttacga aattaattta tgattattga
     1621 ttaattatcg gcaacaaaca tcaaacatcg aaaacatgga aaaaataatg tcaacaattt
     1681 ttgccaaatc gggcttggca tcagaaaaaa ataggtttat attcccacct acaaatttgt
     1741 tttcccatta gtacactatc aaccaaaagg agtatccgaa tgactgacct gaacaccctg
     1801 tttgccaacc tcaaacaacg caatcccaat caggagccgt tccatcaggc ggttgaagaa
     1861 gtcttcatga gtctcgatcc gtttttggca aaaaatccga aatacaccca gcaaagcctg
     1921 ctggaacgca tcgtcgaacc cgaacgcgtc gtcatgttcc gcgtaacctg gcaggacgat
     1981 aaagggcaag tccaagtcaa ccggggctac cgcgtgcaaa tgagttccgc catcggtcct
     2041 tacaaaggcg gcctgcgctt ccatccgacc gtcgatttgg gcgtattgaa attcctcgct
     2101 tttgaacaag tgttcaaaaa cgccttgacc accctgccta tgggcggcgg caaaggcggt
     2161 tccgacttcg accccaaagg caaatccgat gccgaagtaa tgcgcttctg ccaagccttt
     2221 atgaccgaac tctaccgcca catcggcgcg gacaccgacg ttccggccgg cgacatcggc
     2281 gtaggcggac gcgaaatcgg ctatctgttc ggacaataca aaaaaatccg caacgagttt
     2341 tcttccgtcc tgaccggcaa aggtttggaa tggggcggca gcctcatccg tcccgaagcg
     2401 accggctacg gctgcgtcta tttcgcccaa gcgatgctgc aaacccgcaa cgatagtttt
     2461 gaaggcaaac gcgtcctgat ttccggttcc ggcaacgtgg cgcaatacgc cgccgaaaaa
     2521 gccatccaac tgggcgcgaa agtgctgacc gtttccgact ccaacggctt cgtcctcttc
     2581 cccgacagcg gtatgaccga agcgcaactc gccgccttga tcgaattgaa agaagtccgc
     2641 cgcgaacgcg ttgccaccta cgccaaagag caaggtctgc aatactttga aaaccaaaaa
     2701 ccgtggggcg tcgccgccga aatcgccctg ccctgcgcga cccagaacga attggacgaa
     2761 gaagccgcca aaaccctgtt ggcaaacggc tgctacgtcg ttgccgaagg tgcgaatatg
     2821 ccgtcgactt tgggcgcggt cgagcaattt atcaaagccg gcatcctcta cgccccggga
     2881 aaagcctcca atgccggcgg cgtagcaact tcaggtttgg aaatgagcca aaacgccatc
     2941 cgcctgtctt ggactcgtga agaagtcgac caacgcctgt tcggcatcat gcaaagcatc
     3001 cacgaatcct gtctgaaata cggcaaagtc ggcgacacag taaactacgt caatggtgcg
     3061 aacattgccg gtttcgtcaa agttgccgat gcgatgctgg cgcaaggctt ctaagcaaac
     3121 gccgccgctc cgcaaacaaa atgccgtccg aaccgcaaat gctgttcaga cggcatttca
     3181 ttatccgccc gttcgaatcg ggtgatacta tttgtatcca gaattgttgg atagtttggg
     3241 agtggagagg aagaaatgat tatggaccaa tactatatgg agttaaagaa taagttatct
     3301 aacagaccaa tcctactgga taatactaat gattttcttt ttgttttagt aaatacagtg
     3361 aaagctatga tagaaaatac tgataaaagt cagctttcag agttagataa aattttagac
     3421 ggtgtcacaa gtcaagaatt gaaacttgcc tatgattttt gtcaagggaa attcggtcaa
     3481 gcaggttttt cttatcgtag gcacccgaat tatttttatt tgtccagttt aattgcgact
     3541 tttccagaat ttgagttgtc taaagcagac cgagattacc tcaaaggaat cataaatttt
     3601 gataactacc ttttatatga gttagattag tctttaatca cctcaaaacc ctatttattc
     3661 aaaaggagaa aaaatgattg agctgaaatt agtagatgag agcagttttc aggcagtgct
     3721 ggatttgaaa atatctgaag ctgatgaacg agcacgtttt gtggctccaa atgtacgctc
     3781 tttagctgac gcatggctct accgggaaaa tgaagatgtg tttccgaggg caatctattg
     3841 ggataagcaa gtggttggct ttctcctgct ggaaatagac aaggatgaag cagaatactt
     3901 tatctggcgg ataatgattg gtcagcagtg
//
LOCUS       DAWHXZ010000179         3883 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000179 DAWHXZ010000000
VERSION     DAWHXZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.179"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..400
                     /locus_tag="WIE35_001734"
     CDS             <1..400
                     /locus_tag="WIE35_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEZ4154617.1"
                     /translation="GAEFLLDECKRHDVKFLLVSGGFTFFTERLQQRLGFEYQHANVL
                     EIENGRLTGRLKGRIIDAQAKADLLREYRSRLGLQPHQVLAMGDGANDIPMLKEAGIG
                     VAYRAKPKVQAAADACINFGGLERVRGLFG"
     gene            464..1687
                     /locus_tag="WIE35_001735"
     CDS             464..1687
                     /locus_tag="WIE35_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ4154618.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGTDGVS
                     ISFREGVAQASGMRRVAVLTLCGAVAGSGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAVFFQRTAQKFPFIKRDNIKIIPLAVCMFALIGVIAVWFP
                     EILGNGKAGNQLTFGGLTGWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSGSAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            1848..3428
                     /gene="purH"
                     /locus_tag="WIE35_001736"
     CDS             1848..3428
                     /gene="purH"
                     /locus_tag="WIE35_001736"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ4154619.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLTKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLGEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDILSGTPEIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDICR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRITARKAQDANFDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTGIRHFRH"
BASE COUNT          885 a         1126 c         1088 g          784 t
ORIGIN      
        1 cggtgcggaa ttcttgttgg acgaatgcaa aaggcacgat gtgaaattcc tgctggtgtc
       61 cggcgggttc acatttttta ccgaaaggct gcaacaacga ctcggcttcg aataccaaca
      121 cgccaatgtt ttggaaattg aaaacggcag gttgaccggt cgtctgaaag gcagaatcat
      181 cgacgcgcag gcaaaggcgg atttattgcg cgaataccgc agccgcctcg gattgcagcc
      241 gcatcaggtg ttggcgatgg gcgacggtgc gaacgatatt ccgatgctca aagaagcggg
      301 cataggcgtg gcttaccgtg ccaaaccgaa agtgcaggca gccgccgatg cctgtatcaa
      361 cttcggcggt ttggagcgtg tacgcggcct gttcggatag gcggatagga aacggatgcc
      421 gtctgaaagg ttttcagacg gcatttgaac ggcaggaacg acagtgggac gcagaaaact
      481 ttggtttgcc ctggcagcag cgggcgttat cggcggtttg gtcggcattg tgctgacgga
      541 actgatgcac ttcatacaac atacggcata cggttatggc acggacggcg tgtccatctc
      601 gttccgcgaa ggtgtggcac aggcttccgg tatgcggcgc gttgccgtgc tgacgctgtg
      661 cggcgcggtc gcaggcagcg gctggtggtt gctgaaacgt ttcggcaagc cgcaaatcga
      721 aatcaaagcc gccttgaaac agccgttgca ggggctgccg tttctgacga cggttttcca
      781 tgttctgctg caaatcataa cggtcggact cggttcaccg ctcggacggg aagtcgcccc
      841 gcgtgaaatg actgccgcgt tcgcttttgc cggcggcagg cggttgggtt tggatgaaga
      901 cgaaatgcgg ctgctgattg cttgcgcttc gggtgcgggt ttggcggccg tgtataacgt
      961 gccgctcgcc tccacacttt ttatcctcga agccatgctg ggcgtgtgga cgcagcaagc
     1021 cgtcgccgcc gcattgctga cttccgtcat cgccaccgcc gtcgcgcgca tcggcttagg
     1081 cgacgtgcag caatatcatc ccgccaacct tgccgtaaac acttcattac tttggttttc
     1141 cgccgtcatc ggcccgatac tgggcgcaac cgccgtcttt ttccagcgca ccgcccaaaa
     1201 attccccttc atcaagcgcg acaacatcaa aatcattccc ttggcagtct gcatgtttgc
     1261 gctcatcggc gtgattgccg tttggtttcc agaaattttg ggcaacggca aagcaggcaa
     1321 ccaactgacc tttggcggtt tgaccggttg gcaacacagc cttgagctga ccgccgtcaa
     1381 atggctggtt gtcttgatgg cgttggcggc aggcgcatac ggcggactga ttacgccgtc
     1441 tatgatgctc ggcagcacca tttcttttgc agccgccgcc gcgtggaaca gcgttttccc
     1501 cgaaatgccg tctggaagcg cggcagtcgt cggagcggcg gtgtttctcg gcgtatcgct
     1561 caaaatgccg ctgaccgcca ttgtgttcgt attggaactg acctacgcgc cgctgtccct
     1621 gctgctgcct ttgtgcatcg gcatggcggg ggcattggga acatcgggaa aaatgggctt
     1681 caaataaatg ccgacaaatg ccgtctgaaa ggttttcaga cggcatttct ttgtaaattg
     1741 tcagcaatat ctgcaaaaac ctactgccaa aaaactagaa atagcggata ataccgcccc
     1801 gaaaaagttc ccctttcccc atccaactgt cagaaaagga agccccaatg tccgtcatca
     1861 aacgcgccct gatcagcctg tccgacaagg caggcgcggt cgaatttgcc caaaccctga
     1921 ccaagctcgg tgtcgaaatc ctctccaccg gcggcacggc gaaactgctt gccgatgcgg
     1981 gcgttcccgt gattgaagtc gccgactata ccggcttccc cgaaatgctc gacggccgcg
     2041 tgaaaacgct gcatccgaaa atccacggcg gtattttggg tcggcgcgat ttgggcgaac
     2101 acgtcgccaa gatggaagaa cacggcatcg gcaatatcga ccttgtgtgc gtcaaccttt
     2161 atcccttcgc cgccaccatc gccaaaccaa actgcacact ggaagacgcg attgaaaaca
     2221 tcgacatcgg cggcccgacc atggtgcgtt ctgccgcaaa aaactggaaa cacgtcgcca
     2281 tcgttaccga caccgccgac ttccccgcca tcgccgccga actcgaagcc aacaacggcg
     2341 cgttgagcga caaaacccgc ttcaacctgt cgcgcaaagc gttcagccac accgcccaat
     2401 acgacggcat gatttccaac tacctgacct cgctttcaga cgacatcttg agcggcacgc
     2461 ccgaaatcgg tgaattcccg agccagttca accaaagctg gattaaagtg caagacatgc
     2521 gctacggcga aaacccgcat cagcgcgccg cgttctaccg cgatattgac cccgccgcag
     2581 gcagcctttc cgcctacaaa caactgcaag gcaaagaatt gtcttacaac aacatcgccg
     2641 atgccgatgc cgcatgggaa gccgtcaaat cctttgacgc gccagcctgc gtgatcgtca
     2701 aacacgccaa tccgtgcggc gtcgccgttg cagccgatac attgaccgcc tacaaactcg
     2761 cctacgtcac cgacaccacc agcgcgttcg gcggcatcat cgccttcaac cgcgaagtgg
     2821 acggcgaaac cgtcaaacag attaccgaca accagtttat ggaagtcttg atggcgccga
     2881 agtttaccgc cgaagccctc gaaatcgccg ccgccaagaa aaacgtgcgc gtattggaag
     2941 tgccgcttga ggcaggcgca aaccgcttcg aactcaaacg cgtcggcggc ggactgttgg
     3001 tacaaacgcc cgacatctgc cgcatcagcc gcgccgattt gaaagtcgtc tccaaacgcc
     3061 aaccgaccga gcaggaatgg aacgatttgc tgttcgtctg gaacgtcgca aaatacgtca
     3121 aatccaacgc catcgtgttc ggcaaaggcg gacaaaccta cggcatcggt gcaggccaaa
     3181 tgagccgcgt ggacagcacc cgcatcaccg cccgcaaagc gcaggacgcc aatttcgacc
     3241 tcaacggcgc gtgtgccgcc tccgatgcct tcttcccctt ccgcgacggc gtggatgtga
     3301 ttgccgaaca gggcattaaa gccatcatcc atccggcagg ttcgatgcgc gatcaagaag
     3361 tcttcgacgc ggcagacgaa cacggcattg cgatggtggt aaccggcatc cgccacttcc
     3421 gccattaagg cagatgaaca aggcaatgcc gtctgaaggg ctttcagacg gcattttgcg
     3481 ctattttgtg aaggtaggga tgacggttcg ggtattcctg acagggtgga ttttcaaggt
     3541 gttgtatagg gtgtaggagg attcgtaaaa ggtgggatgc agggtgtgct tcagcccgct
     3601 gcatcaaaaa tttttggaga accggcggga gtcggcggtt ttggtttcgg cggggacggt
     3661 ggaaatgggt aacattgacg gaatcgacgg aagcggtggg ctgaagccca cccttgtata
     3721 ttggaatcac tgtatcatag caacaaaccg cccggccgcc acccgcgccc acccaaggca
     3781 cacaaccgtt gcgtagctca gggagcggca gggcaaccca tcgacacaac cggacagttg
     3841 ccggacaaca caaccgaatg caaggcaggt ttatgatgag tac
//
LOCUS       DAWHXZ010000180         3873 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000180 DAWHXZ010000000
VERSION     DAWHXZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.180"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1161..1598)
                     /gene="rnhA"
                     /locus_tag="WIE35_001737"
     CDS             complement(1161..1598)
                     /gene="rnhA"
                     /locus_tag="WIE35_001737"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ4154620.1"
                     /translation="MDQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            1712..2581
                     /gene="tehB"
                     /locus_tag="WIE35_001738"
     CDS             1712..2581
                     /gene="tehB"
                     /locus_tag="WIE35_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ4154621.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKAENLPEILLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKLKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAARDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAESLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPEVIADMQAHTAVG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVKYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(2631..3437)
                     /gene="thiD"
                     /locus_tag="WIE35_001739"
     CDS             complement(2631..3437)
                     /gene="thiD"
                     /locus_tag="WIE35_001739"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ4154622.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETIFAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
BASE COUNT          823 a          963 c         1097 g          990 t
ORIGIN      
        1 tttaatttgt ctgtccgcat tgccgctaat tgtaccactt catcgtattt ccttttcggt
       61 tgaaaccccg ccactcggac atctgtcctt cggggcggca ggatcagact ttatttggga
      121 gggggtgtaa ccccttccaa atcaggacga cacataggga ggtgctttat gtgtcgtcct
      181 gtgtgttgaa atatatcgga cgcgtttgaa cagttgtgcg gcaaactgtt ttagcggcag
      241 gcaagagatg ccgtctgatg ccgtctgaag cccattgtcc gtcattccca cacaggcagt
      301 tgctacggtt gctgaagccc cttagccgtc gcgcaggcgg ttgctacggt tactaaaatc
      361 ccgtcattcc cgcgcaggcg ggaatccagt ccgttcggtt tcagttgttt ctgataaatc
      421 ttgcagcgtt gggtttttag attcccgctt tcgcgggaat gacggggcgg tcggatgccg
      481 cctgaaactc cgtcattctt gcaacttttc gtcattcccg tgaaaacggg aatctagaac
      541 ttcaaacttt cagataatct ttgaatattg ccgttgcccg acggtctaga ttcccgcttg
      601 cgcgggaatg acggtagagc ggtttcagtt gctcccgata aatgccgcaa tctcaaatcc
      661 cgtcattccc tcaaaaacag aaaaacaaaa tcagaaacct aaaatcccgt cattcccgca
      721 gctcttcgtc attcccgtga aagcgggaat ctagaccttt taaattttca cacggctttt
      781 gaatattgcc gttacccgaa ggtctggatt cccgcctgca cgggaatgac ggcagagcgg
      841 tttcagttgt ttctaaataa atttttgcag cgttggattt ttagattccc gctttcgcgg
      901 gaatgacggg gcggtcggat gccgcctgaa gcccgacaaa gcccccaatg ccgtctgaaa
      961 cctcgtcatt cctctgctgt ccgcaacttt tcgtcattcc cgcaaaagcg ggaatctaga
     1021 gcctctaaat tttcagacgg cttttgaata ttgccgttgc ccgacggttt ggattcccgc
     1081 ctgcgcggga atgacggtgg aggacgatgc cgtctaaagc ccattagcgg tttcagacgg
     1141 catttttgcc ggagcggcag tcaggaaaac tgcgctgcgc cacggtttgc caaatcgtcg
     1201 gcgcgttcgt tttccgcgtg tcccgcgtgt cctttaaccc aagtccagct gacttggtgc
     1261 tgcccaacca gaacgtcgag ttctttccac aagtcgtcgt ttttgacggg ctgtttggcg
     1321 gcggttttcc agccgttgcg cttccaaccg tgtatccagt tttccatgcc gtttttgacg
     1381 tattgcgagt cggtgcagat gatgacggtg cagcggcgtt tgagcgattt cagtccttcg
     1441 atgacggcgg tcagctccat gcggttgttg gtggtttgcg cttcgccgcc gaaaagttct
     1501 ttttcgtggc tgccgtagcg catcaacacg ccccagccgc ccgcgccggg attgcctttg
     1561 cacgcgccgt cggtgtaaag gtaaacggtt tggtccatta ttgtgccttt gtgtggtgtc
     1621 cggcgtgatt ataagcggtt tgtgcgccgt gccgtctgaa agattgcggc ggattaaaat
     1681 aggcatataa tgtgcaactt tatttttgaa tatgaaggag cggatagtgg gtcaaagcgg
     1741 cgagttgttt tgtttcggac aaatgcccgt atggaaggcg gaaaacctgc cggaaatttt
     1801 gttgtcgggt tattcgtccg aaggagggga atgggtctgc ctgaatgtgt tgcagggcga
     1861 tgtcgaagtc cgcacgcagg acggggcggc agaggtttgg tcggcggaaa gcggcgattg
     1921 cgtgtttgcg ccgcagcagg tgttttcggt caaactgaaa acggacgatg ccgaaatccg
     1981 tttgtcgctg tattgcgcgg cggcagacta ttttcacaaa aaatacggga tgagtgccac
     2041 gcattctgcg gtcgcggcgg cacgggatac cgtaccggcg ggcagggcgt tggatatggg
     2101 ctgcggacag gggcgcaacg cgctgtttct cggtttgaag gggtttgaag ttactgcagt
     2161 cgatcacaat cccgccgcct tggcaaacgt ggcggagctg gcagaggcgg agagtttgaa
     2221 cgtccgcacg ctggaatatg atttgaacgc cgccgccttg cagggcgagt ttgattatat
     2281 tgtggcaacg gtggtgctga tgttcctgat gccgcagcgc gtgcctgaag tgattgccga
     2341 tatgcaggcg catacggcgg tgggcgggta caacttaatc gtatcggcaa tggatacggc
     2401 ggatttcccc tgtccgatgc cgttcccctt caaatttaaa gagggagagc tgaaggatta
     2461 ttatcgggat tgggagctgg tcaaatataa agaagaattg ggcgcgatgc acgccaaaga
     2521 cgaaaacggc aatccgatac ggtttaaatt tgtaacgatg ctggcgaaaa agcctgaata
     2581 atcctcaaaa atgccgtctg aacattttgt ttcagacggc atttttacgt ctagtcccga
     2641 tacgcccaat gattgaccgg cccgtgtcct gcgccgattt ccaaagggtt tgagattgcc
     2701 gccgtgatgt atgccttggc agtctgtacg gctttgcaaa cgtctaagcc ttttgccaac
     2761 tcggcggtaa tgcaggcaga aaacgtgcag cccgtgccgt gcgtgtgggc ggttggaaag
     2821 cgcgggctgt ccaattccag cgtttcattt tgcgtaaaca gccaatccgt gcagcgtccg
     2881 cttgtgctgc cgttcaaatg tccgcctttg ataacgacat ttttgacacc gtaatcaagc
     2941 aggatttttg ccgcacgttc cgcatcttta cggttttcga tatgcacgcc ggtcagggct
     3001 tccgcctcgg gcaggttggg ggtcaataca tccgtatcgg gaagcagcag gcgcgtcagt
     3061 gccgcaacgg cggaatcctg caacagcggc gcaccgcctt tggcaatcat cacggggtcg
     3121 agtacgcgcc tgccgaagtc gcagtgtttc agtttgtcgg caacgcattc gatgatttcc
     3181 gccgtgccga gcattcctat tttgtaggcg cggatgtcaa agtcttccct gattgcttgg
     3241 atttgtgcga agatggtttc ggtcgggacg agatgaaccg ccgacacgcc caaggtattt
     3301 tgcgcggtaa cggcggtgat gacgcacgtt ccgaacacgc cgcgcatctg aaacgttttc
     3361 aaatccgcct gaatgcccgc accgccgccc gaatccgaac cggcgatggt aagcgtttgc
     3421 acaaaagagc ttttcatttg ttcctcctta atattgtgtc cgataaaaag ggctaatgcc
     3481 tgtaaaaaag cacggcgatg ccgtctgaaa accgcagcgc ggggtttgtg gtgcattata
     3541 tgccgtattg caggacaagg cacagggcag ggcggcgggg tgcatcgtag caactgtatt
     3601 tttgcccgac ggggtgaaaa atacagttgc tacacccact gataatgaaa aaagagagca
     3661 atgccgtctg aaccgcgtgt tgcggcgagc ataaaccggc acgctttatt cgtcattccc
     3721 acaaaaacag aaacctaaaa ttccgtcatt cctacgaaag cgggaatcca gtgtgttcgg
     3781 tttcagttgt ttttgggttt cgggtgattt ccaaatcatc attcccgcgc aggcgggaat
     3841 ctagcccttg gggcagcggc aatatttata aag
//
LOCUS       DAWHXZ010000181         3861 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000181 DAWHXZ010000000
VERSION     DAWHXZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.181"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WIE35_001740"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WIE35_001740"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ4154623.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGIAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDDGGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WIE35_001741"
     CDS             1439..1924
                     /locus_tag="WIE35_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ4154624.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WIE35_001742"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WIE35_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ4154625.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGCFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLSGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          791 a          949 c         1072 g         1049 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cctcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 tcgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaga tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgttgct gagcgcggct ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcatcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaagttttag atgacggcgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgcgcctt cttcgcccaa
     2041 acggcgtttc ttatcggctt cgtattcctg atagttgccg tcgaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccaac ggtcgtgaga
     2161 aatcaccatc acgctgccgg caaattccaa caatgcgtct tccagcgcgc gcagggtttc
     2221 cacatcgagg tcattagacg gttcatccag cagcaataca ttgccgccgc tcaacaaggt
     2281 tttcgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcatcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttgaacagc gtagatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttatcg ccgaacgatt tggaaacatt tacaaattca atcacttcgt tacccaaacg
     2701 ctcggcaacg ggaataaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcctca aaacgcgcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgcgttacc gcaacgactg tgccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 cgattccgca tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gcttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc agcgatttcc aattcatgct ctgcaccgcc
     3241 gcccgtggac gaaccggccg cgataatcgc ttccaagcgg ccctgctctt ccgccaacgc
     3301 gtcaaaatcc gcatcgggat tggcatactc ggcatacact tcttccaagc gtttctgcgc
     3361 cgccgccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt gatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacgcccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taataatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg ccccggcaac
     3841 catttcggat tttccaaagc a
//
LOCUS       DAWHXZ010000182         3833 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000182 DAWHXZ010000000
VERSION     DAWHXZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.182"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(135..1052)
                     /gene="thrB"
                     /locus_tag="WIE35_001743"
     CDS             complement(135..1052)
                     /gene="thrB"
                     /locus_tag="WIE35_001743"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ4154626.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACTRLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKVFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1089..1817)
                     /gene="ubiG"
                     /locus_tag="WIE35_001744"
     CDS             complement(1089..1817)
                     /gene="ubiG"
                     /locus_tag="WIE35_001744"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ4154627.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1969..3210
                     /locus_tag="WIE35_001745"
     CDS             1969..3210
                     /locus_tag="WIE35_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ4154628.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPVGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRQKFGAGKTYKVYGGLWLMVWVFLF
                     GIINIAAQVLSQMELVPVFKG"
     gene            3360..>3833
                     /locus_tag="WIE35_001746"
     CDS             3360..>3833
                     /locus_tag="WIE35_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ4154629.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQHRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIMAHSHMMQRELVGLYGVPPER"
BASE COUNT          914 a         1224 c          878 g          817 t
ORIGIN      
        1 gccttgtcct gatttttgtt aatccactat aaaatggaaa atacccggct atcgtctcat
       61 tttcgtttta atcagccata aaaatgccgt ctgaaaccct ttcagacggc atttctgtca
      121 aacgccggac gcactcaatc caaactcaac agcaggttgc ggaacgcgtt cgggtctttg
      181 ataaacgtca tctcgcccgc ctgcggaaaa tgaaagtcca acaggcgcga cacccaaaaa
      241 cggatgcagc cggcacgttg ggcggtcggg aaatacgcct tttcttcggc actcaagggg
      301 cgcacgccct cataaccgcc gataaacacc tttttcaacg cctcatccaa cttattgtcc
      361 gccgtccttg cccaatcgtt gaccgcaatc gccaagtcat acataaaatt gccccggcag
      421 gcataataga aatcgatgaa acccgatacc tgaccgccgt caagcaacac attgtcttta
      481 aacaaatcgg catggatgat gcccgaaggc agatgattgc cgagattgtc ctttaacgca
      541 tcgatttcgg aacacagcag cgcggcatcg tcttgcgaca ggacgggcag cagccgggtg
      601 cacgcctccg tccaccacgc atcgtaacgc gggttttcca tttccaaagg gaaatcggcg
      661 gcggcaaggt gcattttggc cagcatcgca ccggtatgga aacactgctc cgccgtcggc
      721 agcgcggtat ccgaaccttt caggcaggca accaaacaag ccggcttgcc cgccaaaacg
      781 gaatcaagcc ggccgtcttt gcgcgcaacc ggcgcggcaa ccgccacgcc cttcatactc
      841 aaatgccggt taagctccag aaaaaacggc agctcttcct gtttcaacac ttcaaacacg
      901 gtcagcacat aacgtcccga agtcgtcgtc agaaaataat tgctgttggt aatcccctgc
      961 gcgatgccct gcagggaaac aaattccccc aaatcgtaac cgctcaggaa gccgcgcatt
     1021 tcatcatcgg aaacactggt atagacagac atatcaactc acttgctcaa aaacgggcaa
     1081 acccgccgtc agaacgccgg acggcaggca aacatataat tcacatcggt cgaatcgcac
     1141 agggcataag tttgcgacaa cacatggtaa gtcataccct tcgtatccgc cacatccaag
     1201 cctgcctgac ggcacattcg cgccagctcg gcaggtgcga tgaatttttt ccagtcgtgc
     1261 gtgcctttgg ggacaaactt caacagatat tccgccgcca caatcagatg caggtacgat
     1321 ttcgggtttt tattgatggt ggaaaaaaac accatgccgt ccggcttgac cagcttggca
     1381 caagcacgca cgatggcggc gggatcgggg acgtgttcca tcatttccat gcacgttacc
     1441 acatcgaacg agtgcggttc cgcctcggca aggtcttcca cgcggataca ttcgtattcg
     1501 atatcggcga cattgttcaa agccgcgtgc aggcgggcgg tttccaacga ctgctccgcc
     1561 atgtcgatgc cctttacaaa cgccgcgccg cgccgtgcca tactttccgc caagatgccg
     1621 ccgccgcagc ccacgtccaa aacccgtttg ccgcacaaat ccgcgtgccc gtcgatataa
     1681 tccagccgca gcggattgat gtcgtgcaag gttttgaact cgcccgactt gtcccaccat
     1741 ttgtcggcaa tccggctgaa tttggcgatt tccccctcat cgacattata ttttttgtcg
     1801 gacattttcc ctcccatctg acgaaccgcc cactccaaaa cccaagatta tactatggaa
     1861 cacaatgccg tctgaaagcc ttttcgggcg gcgcggcgca aatgccttac aaatccttac
     1921 actttacggc ataatggcgg ctcgcttttt ctggcagaaa gacaaaatat gcccaccaaa
     1981 accccttcac tgttcggcgg cgcgatgatt atcgccggca cggtcatcgg cgcaggtatg
     2041 ctcgccaacc cgaccgccac atccggcgta tggtttaccg gctcgctggc cgtgttgctg
     2101 tacacctggt tttctatgct ttccagcggc ctgatgattt tggaagtcaa cacccactat
     2161 ccccacggcg caagtttcga cacgatggtc aaagacctgc tcggacgcgg ctggaacatc
     2221 atcaacggca tcgccgtcgc cttcgtttta tacctgctca cctacgccta catcttcgtt
     2281 ggcggcgacc tgaccgccaa aggcttaggc agcgccttgg gcggcaatgt ttcactcacc
     2341 gtcggacaac tcgtcttctt cggcatcctc gccttttgcg tatgggcatc cgcacgcttg
     2401 gtcgaccgct tcaccagcgt cctcatcggc ggcatggtat taacctttat ttgggcaacc
     2461 ggcggcctga ttgccgatgc caaaatgccc gtcctgttcg acacccaagc ccccgtcggc
     2521 accaactact ggatttacgc cgccaccgcc ctgcccgtct gccttgcgtc attcggtttc
     2581 cacggcaacg tctccagcct gctcaaatac tttaaaggcg acgcgcccaa agtggctaaa
     2641 tccatctgga cgggcacact gattgcgctg gtaatttacg tcctctggca aaccgccatc
     2701 caaggcaacc tgccgcgcaa cgagttcgcc cccgtgattg ccgccgaagg gcaagtctcc
     2761 gtcctcatcg aaaccctgtc caaattcgcc caaaccggca atatggataa aatattgtcc
     2821 ctgttttcct atatggcgat cgccacctcg tttttaggcg taaccttagg tctgttcgac
     2881 tacatcgccg acatcttcaa atggaacgac agcgtgtccg gccgcaccaa aaccgccgcg
     2941 ctgaccttcc tgccgcctct aatttcctgc ctgctcttcc ccaccggctt cgttaccgcc
     3001 atcggctacg tcggcctggc ggcaaccgtc tggacaggca tcatccctgc catgctgctc
     3061 taccgctcgc gccaaaaatt cggcgcaggc aaaacctata aagtttacgg cggcttgtgg
     3121 ctgatggttt gggtcttcct tttcggcatc atcaacatcg ccgcacaggt attgagccaa
     3181 atggaactcg tccccgtatt taaaggataa aggcaaaatg ccgtttgaag cccgccggcg
     3241 gcttcagacg gcattgccgc aacaaacggc aaccgtattc cggcacacag cgcattaccc
     3301 tgcccctcac gcacaaatcc cgccccgaca aaccgggacg caaccataaa ggaacaatga
     3361 tgaagctcaa aatagatatt gcaaccaaca acttcaaaca cggcggcggc acggaacgct
     3421 acacattgga tttggtaaag ggtctgaaca gacaaaacat cacaccggcc gtttatgcga
     3481 cgaaatttga tcacggcatt cctgaatacg ccatgatcga accccatctt gtcgatcaac
     3541 accggacgct gaaaaaacta cgctcattcc tcttttcaag ccggctcgct caaaccagaa
     3601 aaaacagtgc cgccaaactg attgcctgcc accacgccga ttacgccgac ctcctcatct
     3661 gcggcggcac acacttgggc tacctgcacc atatggcgca aaaaccgaac ctgctcgacc
     3721 gcctcgccat acgccgcaac cgcagcaact acgccaccgc caaactgatt atggcgcatt
     3781 cccatatgat gcagcgcgaa ctggtcggac tatacggcgt tccccccgaa aga
//
LOCUS       DAWHXZ010000183         3827 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000183 DAWHXZ010000000
VERSION     DAWHXZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.183"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(88..906)
                     /locus_tag="WIE35_001747"
     CDS             complement(88..906)
                     /locus_tag="WIE35_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ4154630.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
     gene            complement(986..2236)
                     /locus_tag="WIE35_001748"
     CDS             complement(986..2236)
                     /locus_tag="WIE35_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ4154631.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="WIE35_001749"
     CDS             complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="WIE35_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ4154632.1"
                     /translation="MFDFPPISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHLGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHGGGGRKCLLTAHTERGRESLPL"
     gene            2889..3701
                     /locus_tag="WIE35_001750"
     CDS             2889..3701
                     /locus_tag="WIE35_001750"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ4154633.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALSTTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          847 a          932 c         1146 g          902 t
ORIGIN      
        1 cactataacc gaaggtaagg gaggggtgtg tccactgtgc cgccgcattt gccgcgtgcg
       61 gaagatacgg ataacaaccc cttggaatta aagggagaag ttgcgggaaa tatccatatc
      121 aggctgtggg ccgtacttat ttacggtatt ttcgtatgcc gaaataaaat atttcttgaa
      181 taaaccgtaa tcgccggttt cgtaataggc tacgacagcc tcggcatagg cgggataact
      241 gtcgggggag aatacgcaag gaaaaaaacc ggagcgcatc agcgacaagg tcatgcagtt
      301 tctggcagta cgtttattgc aatctttaaa atattgaaga tacgccaaat tattatggag
      361 ataaacggcg cggtcgaaag gattttctat tttgggagct tcttgaagca accattttaa
      421 ttccgtatcc aaactttgcg ggttagataa aggggtgtag tctgtcccgc ttatggtaac
      481 gctgtcgcgg cggactacgc cgccggagcc tttttccagt aagttttcgg aaatcaggct
      541 gtgggtggtt tttaagaaat ccaaccagtc aaacggctcg gggctgtcca aaccggacag
      601 caggtgccgg taactttccc tcaggttgat taacattacg gcatccgaat ataacttccc
      661 gcctgcggtt tgcccgagtt taagcaatgc ctgcgtatcg tattgattat aagtgtttcc
      721 ttccaatttg gcagaactgt acacaaaatc gataccgatt tcttcaatat tacgcacgga
      781 gtccgttaaa acagataaag gaagccgatc ggacaattcg gtcaggtatt cttgttcgcc
      841 ggctgtaaaa atggggcgga tatttaaaaa atcctgttgg tatctcaatt tatccatacc
      901 tttcatcata aaaacgcggt ttggggcgga agtaggttta tgcctgcctt catccgacaa
      961 ctatattgaa acaccgcctt ttcaatcaac cccgcttcaa tacggatgta ttgatgtagc
     1021 gttggacacc cgaggcaatg gattgggcgc actgccgacg gaaggattcg ctgcccagca
     1081 gcttctcttc ggcaggattg gacaggaagg cggtttcgac caggatagac ggcatatcgg
     1141 gcgcgcgcaa aacggcgaaa ttggcttcgt ccaccctgcc tttgtgcaga tggttgagcc
     1201 tgcccaattc ttcaagcacc agtttgccga gtttgcggct gtcgcgcagc gtggcggttt
     1261 gggtcatatc gagcagggcg gtatcgacat tgcggttgcc gctggtcggt acgccgccga
     1321 ccgcgtcggc attattttgg gtttgttcca agaatttggc ggcggagctg gtcgcgcctt
     1381 tggtgttcag catataaacc cctgtgccgc gcgcggaggg gctggtgaaa gcgtcggcgt
     1441 ggatggagac gaatacgtcc gcccgccgtg ctcgcccttt ggcgacacgc acgcccaatg
     1501 ggatgaacac gtcttcgttg cgcgtcataa agacgttgta acccaaggct tccaattgtt
     1561 ttttggtttc gcgggcgatg gagaggacga cgtgtttttc ctgtaggccg cccgggctga
     1621 cggcgccggg gtcttcgccg ccgtgtccgg ggtcgatcat aatgacgggt ctgcgcccgt
     1681 ttttgccgcg ttcggtttgt gcgggagtgt tttgagcgag gtcggcttca ggggagccgc
     1741 gacgggtttt gttcaggctg ccgttaagca gcgccatcat cggatcgtcg ggatccatcc
     1801 cttgcggata gagatcgacg acgaggcggt ttttaaagcc gccgacgggg gggagggaaa
     1861 aaacttgcgc gtaggaaggc tgtttcaaat cgatgacgag gcggacggtg gtcggtgtgt
     1921 tctgacccgc gcgtatgctg cggataaaag ggtcgtccgc cataaccttc tgagacagtc
     1981 cgtgcaatac ggtattgatg ttcgcgtttt gtatgtcgac gaccagcctg cccgggttgt
     2041 cgagcgtgaa gtgctggtat ttgagcgcgg cggtgctttc cagcgtcagg cgggtgtagg
     2101 tgtgcgacgg ccatatccgt gcggcggtga attgcggggc gcgtaccgtt ttggcaacgg
     2161 cggatgcgat ggggcttagg gcgaacagtg tgccggcggt gcggcggatg atttgtcttc
     2221 gtgtcagttt ggtcatagcg gcaggctttc gcgtcctcgt tcggtatggg cggtcagcag
     2281 gcattttctg ccgccgccgc cgtgtgtcaa tgttgcggtg atgtcggcgg gcggcgtaaa
     2341 ttccccgccc tgttgcggcc attcgatcag gcagacgctg tttgcggcaa acagttcgtc
     2401 aagccccgcg tcttcccatt cttcggggaa cgagaagcgg tagaggtcga aatggtgcaa
     2461 ggtgaagcgt tccagcggat aagattcgac gatggcgtag gtcgggcttt tgactgcgcc
     2521 cagatgaccc aatccgcgta ggatgccgcg tgtcagcgtg gttttgcccg cacccaaatc
     2581 cccttcgaga taaatgacca gcggtgcgtt taaacgggaa gaccacgccg cgcccaaatc
     2641 gagtgtggcg gcttcgtcgg caaggaatcg ggaaatgggg gggaaatcaa acataggggc
     2701 tttaaaactt ttatgtggcg gatcggagag ggcggccggc aaaccgtgcg cccggcgggc
     2761 cggatgcggc attgccgtac attataacag ccaaatccgc atcctgcttt cagacggcaa
     2821 tggctgtcaa gaaaaagcgg cgcgtgtaca atacgcggat tgtatgtttg ggacggattg
     2881 gaaaaaggat ggaaaatatc ggcaggcagc gacccatcgg cgtttttgac tcgggaatcg
     2941 gcggtttgac caatgtgcgc gcgctgatgg agcggctgcc gatggagaac atcatttatt
     3001 tcggcgacac ggcgcgcgtg ccttacggga cgaaatctaa ggcgaccatc gaaaatttct
     3061 cgatgcagat tgtcgatttt ttattggaac acgatgttaa ggcgatggtt atcgcgtgca
     3121 acacgattgc ggcggttgcg gggcagaaaa tccgtcaaaa gacgggcaat atgcctgtgt
     3181 tggacgtgat ttccgccggc gcgaaagccg cgctgtcaac cacgcgcaac aacaaaatcg
     3241 gcattatcgc caccaatacc acagtcaaca gcaatgctta tgcgcgcgcc atccacaggg
     3301 acaaccccga cacgctcgtc cgcacgcagg ccgcgccgct gctcgtccct ttggtggaag
     3361 agggctggtt ggaacacgaa gttacccgcc tgaccgtatg cgaatacctc aaaccattgc
     3421 ttgcagacgg catcgatacg ctggtgttgg gctgcacgca ctttcccttg ctcaagccct
     3481 taatcggcag ggaggcgggc aatgtcgcgt tggttgattc tgcaattaca acggccgaag
     3541 aaaccgcacg cgtccttgct caggaaggat tgctcaatac cgacaacaac aatcccgact
     3601 accgttttta cgtcagcgat attcctttga aattcagaac catcggcgag cgttttctgg
     3661 gcaggacgat ggagcagatt gaaatggtgt ctttgggtta aaacgatgac ggaaagctgc
     3721 ccgagattac agaaacctaa aatcccgtca ttcccacgaa agtgggaatc tagacctgtc
     3781 ggtgcggaaa cttatcgggt aaaacggttt cttgagattt tgcgtcc
//
LOCUS       DAWHXZ010000184         3796 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000184 DAWHXZ010000000
VERSION     DAWHXZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.184"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..333)
                     /locus_tag="WIE35_001751"
     CDS             complement(<1..333)
                     /locus_tag="WIE35_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEZ4154634.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGVGLARAAVAGRVNGKL
                     VDACDPIVENSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKP"
     gene            complement(387..463)
                     /locus_tag="WIE35_001752"
     tRNA            complement(387..463)
                     /locus_tag="WIE35_001752"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(427..429),aa:Val,seq:gac)
     gene            642..1757
                     /gene="tgt"
                     /locus_tag="WIE35_001753"
     CDS             642..1757
                     /gene="tgt"
                     /locus_tag="WIE35_001753"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ4154635.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAIGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAIEQGKFADWQARFHENRARGTD"
     gene            1839..2849
                     /locus_tag="WIE35_001754"
     CDS             1839..2849
                     /locus_tag="WIE35_001754"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ4154636.1"
                     /translation="MLPFFPEPSLSYTQQNRTAVLLLNLGTPDAPTAQAVRPYLKSFL
                     TDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYEKIWFKEGSPLEVYTARQAAALAER
                     MPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLVIPMYPQYAASSSGAAVDKVCEQLL
                     LQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAEHGRGKKLMLSFHGVPQKHYDLGDP
                     YPDECRHTAKLLAEALELTEDQYVVSFQSQFGRAKWVTPSTQDLFGKLPKQGVTELDV
                     FCPGFLADCLETMEEIALMGREQFYEAGGKSYRYIPCLNDNPDWIDALVALAEENLGG
                     WR"
     gene            3041..3442
                     /locus_tag="WIE35_001755"
     CDS             3041..3442
                     /locus_tag="WIE35_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ4154637.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(3505..3795)
                     /locus_tag="WIE35_001756"
     CDS             complement(3505..3795)
                     /locus_tag="WIE35_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154638.1"
                     /translation="MAKYFTETGISVREYFDFFGEFVVLPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT          993 a         1079 c          923 g          801 t
ORIGIN      
        1 cggtttttcc gtggcgatgt cgtaataaaa gccctcttca atgacggggc cgataaccat
       61 ttttgcatta ggatagagtt gcttgacggc atgcccgaca agatgggcgc aggaatggcg
      121 gatgatttcg atgccttcct gatctttcgg agtaatgatt tgaacagcag aattttcaac
      181 aatcgggtcg cacgcatcga ccaatttgcc gtttaccctg cctgccaccg ccgccctcgc
      241 caaaccgaca ccgatagacg cagcaatttg agccacggta acgggggatt cgtattggcg
      301 gactgagccg tccggcaagg taatgttcaa catcaaaaag ctccaaaaca ttaaaaaata
      361 acggcataaa tgccgttatg ggaatttggt aggcacgatt ggattcgaac caacgacccc
      421 caccatgtca aggtggtgct ctaaccaact gagctacgtg ccttcaaaga atttttgcat
      481 tttatcgggt cgttttaatt tttgcaagag gtatgtgcat ttctttatgc aacattgtgg
      541 caagatttgg ggatgtcgtc tgaaaaaccg tgggaattca tttataatgc cgtcttttaa
      601 aacatatttc gccccctgtc gcccaactga cattcaatct tatgcttaaa tttaccttac
      661 acaaaaaaga cggatacgcc cgacgcggca cgctggagtt gaaccacggc aaaattgaaa
      721 cgccggtatt tatgcctgtc ggtacttacg gctcggttaa ggcgatgaac ccgcaaaacc
      781 tgcacgacat caaggcgcaa attattttgg gcaacactta tcatttgtgg ctgcgtccgg
      841 gcttggaggt cgtcgaacag tttggagggc ttcacggctt tatcggctgg gacaaaccga
      901 ttctgaccga ctcgggcggt tttcaggttt tttctttgtc ggatatgcgc aagctgacgg
      961 aagaaggctg tacgttcaaa agcccgatca acggcgacaa gctgttttta tcgcctgaaa
     1021 tttccatgaa aatccaaacg gttttgaact cggacatcgc gatgcagctg gacgaatgca
     1081 cgccgggcga ggcgacgcgc gaacaggcgc gaaaatcgtt gcaaatgagc ctgcgctggg
     1141 cggaacggag caaaaaagcc tttgaagatt tgaaaaaccc gaacgcactg ttcggcatcg
     1201 tgcaaggcgc gatgtatgaa gatttgcgcg aagagtcgct gaaaggcttg gaagagctcg
     1261 atttccccgg cttggccatc ggcggcttgt ccgttggcga acctaagccc gaaatgtacc
     1321 gcatgttgcg cgccgtcggc cctatcctgc cggaacacaa acctcattat ttaatgggcg
     1381 tcggtacgcc tgaagactta gtgtacggcg tggcacacgg cgtggatatg ttcgactgcg
     1441 tcatgcctac tcgcaatgcg cgtaacggct ggctgtttac ccgttttggc gatttgaaga
     1501 tcaaaaacgc caaacacaag ctcgacaagc gtccgataga cgaaagctgc acctgctacg
     1561 cctgccaaaa cttcagccgc gcctacctgc accatctgca ccgcgccggc gaaatcttgg
     1621 gcgcacagct caacaccatc cacaacctgc atttctacca agtcattatg gcggaaatgc
     1681 gcgatgccat agaacaaggc aaatttgccg actggcaggc gcgattccac gaaaaccgtg
     1741 ctcgcggcac agactgactc ccggcaatgc cgtctgaagc cgcttcagac ggcattttgc
     1801 cgctttaccc tacccgcact atttttcaga aaccgatcat gcttccattc tttccagaac
     1861 cctccctttc ctatacgcaa caaaaccgta ccgccgtttt gctgttgaac ctcggcacgc
     1921 ccgacgcgcc gaccgcgcag gcggtacgac cttatctgaa gtcctttctg accgaccggc
     1981 gcgtcgtcga actgcccaaa tggctgtggt atcccattct gcatggtctg gtactgacgt
     2041 tgcgccccaa aaaaagcgcg cacgcctatg aaaaaatctg gtttaaggaa ggttcgcccc
     2101 tagaagttta cacagcacgc caagctgccg cgcttgccga acgtatgccc gacctcatcg
     2161 tccgccacgc catgacctac ggcaatcctt cggtggcaga cgtattgtcg gaactgaagg
     2221 cgcaaggcgc gggcagactg ctggtcatcc cgatgtaccc gcaatatgcc gcttccagca
     2281 gcggagcggc agtagataag gtgtgcgaac aattactgct gcaacggaac caaatgagcg
     2341 tccgcaccgt ttcgcgcttt tatgacgata ccggctacat tgatgcgatg aaaaaccaca
     2401 tcctccgata ttgggcggaa cacggacgtg ggaaaaaact gatgttgagt tttcacggcg
     2461 taccgcagaa gcactacgac ctcggcgacc cctatcccga cgagtgccgc cacaccgcca
     2521 aactgcttgc cgaagcactg gaactgaccg aagaccaata tgtcgtatcg ttccaaagcc
     2581 aattcggcag ggcaaaatgg gttacaccga gcacgcagga tttgttcggc aaactgccca
     2641 aacagggggt aaccgagctg gacgtattct gcccgggctt tttggcagac tgtttggaaa
     2701 ccatggaaga aatcgccctg atggggcggg aacagtttta cgaagcaggc ggcaaaagct
     2761 accgctacat cccctgcctc aacgacaacc ccgactggat agacgcactc gtcgcacttg
     2821 ccgaagaaaa ccttggcggc tggcgttgaa actgtttgac ccatgtctaa aatgccgtct
     2881 gaacgccgtg ttcagacggc attttcccgc tactttagcg tatgatggca tctatgccgg
     2941 acaacaacat caagtgcaac cttactaccg attcggcaaa aaaatctttc acccgctacg
     3001 caaaatcaac aacaaggttt caaacattaa ggaaacacca atgaacacaa cccgactgcc
     3061 gaccgccctc gtcttgggct gcctctgcgc cgccgcttct gccgccgaca acagcatcat
     3121 gacaaaaggg caaaaagtgt acgaatccaa ctgcgtcgcc tgccacggca aaaagggcga
     3181 aggccgcgga accatgtttc cgccgctcta ccgctccgac ttcatcatga aaaaaccgca
     3241 ggtgctgctg cacagcatgg tcaaaggcat caacggtaca atcaaagtca acggcaaaac
     3301 ctacaacgga ttcatgcccg caaccgccat cagcgatgcg gacattgccg ccgtcgccac
     3361 ttatatcatg aacgcctttg acaacggcgg cggaagcgtt accgaaaaag acgtaaaaca
     3421 ggcaaaaggc aaaaaaaact aaacagacaa aatgccgtct gaaaccggca atccggcttc
     3481 agacggcatt caaatcaaac ctttctaacg cggtactttc agccccatag acccgatatt
     3541 gccgtccccg tcaacctcct tcgcggtcag cataaagccg ccgatgtcaa tacggccgca
     3601 ctcgaccggc tcctgactgt cggaacgctt atcgaaaagc tcggcaaggc tcaaaccctc
     3661 ttcgcccgct tccagcctca aaccgtaagc aagtgccaaa tcacccgaac gtgctgccgg
     3721 caaaacgaca aactcaccga agaaatcaaa atactcacgg acgctaatac ccgtctcggt
     3781 aaagtatttc gccatt
//
LOCUS       DAWHXZ010000185         3735 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000185 DAWHXZ010000000
VERSION     DAWHXZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.185"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            123..2150
                     /gene="uvrB"
                     /locus_tag="WIE35_001757"
     CDS             123..2150
                     /gene="uvrB"
                     /locus_tag="WIE35_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ4154639.1"
                     /translation="MEVIQYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIAQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVMRDRIRNIKESLLFGAE"
     gene            2196..3464
                     /locus_tag="WIE35_001758"
     CDS             2196..3464
                     /locus_tag="WIE35_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HEZ4154640.1"
                     /translation="MKLSKTRIDKSGLALAKQQYKDEIDFIELEECFDLYRQSHLSPL
                     SKTTIDIQNILREYIGEYYIAQRLKRKPQIIRKLNRLSVRLTQLQDIGGCRIIVPTNR
                     DVDKIYKFLSSKKDNLDFLIKKVTDYREKGRDDSGYRALHIIIERENLNFELQIRSRI
                     QHYWAESIEKTSVVYGYYLKEQEGDRDVINYFKELSDVFYEIESGRKPTTIQKLNLED
                     LRKRCEVIIQKSDTHKILNSYVNEGIISALIEKEKRNTNSLNNWILIFDWNAGVFVSW
                     DVVAQDPKQAVEVYVNYENQYPSLEGFEVVLIGSSDIASVQKTHSHYFGIASYENILE
                     TLDESIFCFAKKDDIDIGARRILAAMVRHKFWGTKTVTSETLKNHFCQNLFGFDYSLR
                     DLIRKELIKYGRDKDLSLNIAKKMEIESYL"
BASE COUNT         1175 a          887 c          796 g          877 t
ORIGIN      
        1 tgggtagcga ttgggtttca gacggcatac attcaggatt tcaccgcctt atgccgtctg
       61 aaagacgttt ccgacccatc cctactacac actttgtccg caaacgcatc acaggcaaga
      121 ttatggaagt tatccaatac ccaaattccc ccttcaaact ccaccaaccc ttcccgcccg
      181 caggcgacca gcccaccgcc attgcaggcc tgctcgaagg gctttcagac ggccttgcct
      241 atcaaaccct gctcggcgta accggttcgg gcaaaaccta caccatggcg aacgtcatcg
      301 cccaaagcgg ccgccccgcc atcatcatgg cgcacaacaa aacccttgcc gcccagcttt
      361 acgccgaaat gcgcgaattt ttccccgaaa acgcagtgga atatttcgtc tcctactacg
      421 actattacca gcccgaagcc tatgtgccca gccgcgactt gttcatcgaa aaagacagcg
      481 cgatcaatga acacatcgaa caaatgcgcc tttccgccac caaaaacctg atgacgcgcg
      541 acgacgtgat tatcgtcgcc accgtgtccg ccatttacgg tatcggcgac ccgaccgagt
      601 atcaacaaat ggtgttgtcc gtcaaagaag gcgataccat agcccagcgc gacatcatcg
      661 ccacgctcgt ttccatgcag tacgaacgcg gcgatttgga cttcaaacgc ggcagcttcc
      721 gcgtgcgcgg cgacgtgatt gacgtgtacc ccgccgaaag ctccgaaaac gccttgcgta
      781 tcagcctgtt cgacgacgaa atcgaccgcc tcgatatgtt cgacccgctt tcaggcagcc
      841 ttattcaacg cgtcggccgc tacaccgtct tcccgtccag ccactacgtt accccgcgcg
      901 acaccgtatt gcgcgcctgc gagtccatca aagaagaatt gcgcgaacgc atcgaatttt
      961 tcgcccgcga acaacgcccc gtcgagcaac aacgcatcga acaacgcacc cgcttcgacc
     1021 tcgaaatgct ctacgaaatg ggcttctgca aaggcatcga aaattattcc cgccacttct
     1081 ccggcaaaaa agaaggcgaa ccgccgccca cgctgatgga ctatctgccc gacaacgcca
     1141 tcatgttcat cgacgaaagc cacgttaccg ttacccaaat cggcggcatg tacaaaggcg
     1201 acgcatcgcg caaacaaaac ctcgtcgact acggcttccg cctgccttcc gcccgcgaca
     1261 accgcccgct caaattccac gaatttgaaa aagtcatgcc gcaaaccgtc ttcgtttccg
     1321 ctactcccgc caaatacgaa gaagaacacg ccggacaagt ggtcgaacaa gtcgtccgcc
     1381 ctacagggct ggtcgacccc caaatcatca tccgccccgt cgccacccaa gtcgatgact
     1441 taatgagcga aatcaacgac cgcatccaaa aaggcgaacg cgttctcgtt accaccctca
     1501 ccaaacgcat ggcggagcaa ctcaccgact attacagcga actcggtatc aaagtgcgct
     1561 acctgcacag cgacatcgac accgtagagc gcgttgaaat tattagagat ttgcggcttg
     1621 gactgtttga cgtacttgtc ggcatcaacc tcttgcgcga aggcttggac atccccgaag
     1681 tctcccttgt cgccatcctc gacgccgaca aagaaggctt cctgcgctcg caccgcagcc
     1741 tgattcaaac cataggccgc gccgcgcgca acgtgaatgg cgttgcaatt ttgtatgccg
     1801 acaaaatcac cgactccatg aaagctgcca tcgacgaaac cgaacgccgc cgcgaaaaac
     1861 aaattaaatt caacgaagaa cacggcatcg tgccgcagca gattaaaaaa caggtcaaag
     1921 acatcatcga cggcgtgtac cacgaagaag acagcggcaa aggccgtaga caaggcaaaa
     1981 acaaagttaa agtcggggag attcataacg aagaagacgc gattaaagaa atcgccaaac
     2041 tggaaaaagc catgcagcag gcggctcggg atttgcagtt tgaggaagcg gcggttatgc
     2101 gtgatagaat acggaatatt aaggaaagcc tgttgtttgg ggcagagtaa tgaaatagtt
     2161 taatattctc agcttaatgt ataaaggata attttatgaa attgtcaaaa acaagaatag
     2221 ataaatctgg cttagcattg gctaaacaac aatataaaga tgaaattgat tttattgaat
     2281 tggaagaatg ttttgatttg tataggcaat cacatctttc accattatct aaaacaacta
     2341 ttgatattca aaatattctt agggaatata ttggtgagta ttatatagct cagcgactaa
     2401 aaagaaaacc acagataatc agaaagttaa ataggcttag cgttagacta actcagttgc
     2461 aagacattgg tggttgtaga attattgttc caacaaatag agatgttgat aagatatata
     2521 agtttttatc aagtaagaag gataatcttg attttttaat aaaaaaagta accgattacc
     2581 gtgaaaaagg tagagatgat tcagggtata gagctttaca tataattata gaaagagaaa
     2641 atcttaactt tgaattacaa attagaagta ggatacaaca ttattgggca gaaagtatag
     2701 agaaaacctc tgttgtttat ggatattact taaaagagca agaaggggat agagatgtaa
     2761 ttaactattt taaagaatta tcagatgttt tttatgaaat tgaatcaggg agaaaaccaa
     2821 ctactataca aaagttaaat cttgaagatt taagaaaaag atgtgaagtt attattcaga
     2881 aatcagatac tcataaaata ttaaatagct atgttaatga aggtataatt agtgcgttga
     2941 tagaaaagga aaagagaaat actaattcat tgaataactg gatactaatt tttgattgga
     3001 atgcaggggt tttcgttagt tgggatgttg tagctcaaga tccgaagcaa gccgttgagg
     3061 tatatgttaa ttatgagaat cagtatccat ctttagaggg gttcgaggtt gttttgatag
     3121 gatcatccga tatcgcttct gtacaaaaaa cacatagtca ttattttggc atagcctcat
     3181 atgaaaatat tctagaaaca cttgatgaat caattttttg ctttgctaaa aaagatgata
     3241 ttgatattgg ggcaagaaga atattggcag caatggtaag gcataaattt tggggaacaa
     3301 aaacagtaac atcagaaact ttaaaaaatc atttctgcca aaatcttttt ggatttgatt
     3361 attctttaag ggatttaata agaaaagaat taataaaata tggaagagat aaagatttat
     3421 cattgaatat agcaaagaaa atggaaatag aatcgtattt ataagcaagc gcagcttgta
     3481 tccccatcaa ccgtgtgcgt ggctacgcca cacaccttac ctgcgtatgg cacaaaaact
     3541 ttaaaaccga ggtgtagggt gtgtgcggta cgcacgcacg cgttctttgt agtgcaaacc
     3601 actgcttcaa actttcagac gacctcaaac gccgtctgag aatctatggc ataaaggccg
     3661 tctgaaaaaa tggtttcagt acaactgcca ctggttgtaa cttgattaaa accacccttt
     3721 cagacagcct gccgt
//
LOCUS       DAWHXZ010000186         3695 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000186 DAWHXZ010000000
VERSION     DAWHXZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.186"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(120..1034)
                     /locus_tag="WIE35_001759"
     CDS             complement(120..1034)
                     /locus_tag="WIE35_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ4154641.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WIE35_001760"
     CDS             1290..2759
                     /locus_tag="WIE35_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ4154642.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WIE35_001761"
     CDS             3011..3382
                     /locus_tag="WIE35_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154643.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLDSSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..>3695
                     /locus_tag="WIE35_001762"
     CDS             3455..>3695
                     /locus_tag="WIE35_001762"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ4154644.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYT"
BASE COUNT          801 a          854 c         1052 g          988 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttgtggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ctttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccgga ggcggtaaca cattcggcag cttagacggt ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggtcg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgctga aaaacaggcg gaatgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccgaaag gatgaccggc aacgatgtga tgcagatgct
     3301 ggattcgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcacccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 agcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacgg
//
LOCUS       DAWHXZ010000187         3694 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000187 DAWHXZ010000000
VERSION     DAWHXZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.187"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            351..1472
                     /gene="dnaJ"
                     /locus_tag="WIE35_001763"
     CDS             351..1472
                     /gene="dnaJ"
                     /locus_tag="WIE35_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ4154645.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1668..3686
                     /locus_tag="WIE35_001764"
     CDS             1668..3686
                     /locus_tag="WIE35_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ4154646.1"
                     /translation="MNKSLSSSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDTAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGK"
BASE COUNT          777 a          816 c         1116 g          985 t
ORIGIN      
        1 ggaacctgta aaggaagggg catcggctgc cgccggtgct tttttgtttg gaagggaaat
       61 ggctggaaaa agacctgcgc tatatcgggg aatgaatcga cgcaaaggaa atgccgtctg
      121 aacatgcgtt cgggcggcgt ttttataaca aaaacacttc atggcggttg gttttatgcc
      181 tgtctaagtt tttgtgtcgt gcatacctga ggatttcaga cggcatcggt ttatgctgtc
      241 tgaaaagtgt attccgtttc agtttgtaag ctatggcagt ctgtttgtct tgtgttttgc
      301 gtaattgccc ttattttgag ccgtgatttt attttgaatt agatgaaaaa atgagtaatc
      361 aagattttta tgcgacgctg ggtgtggcaa gaacagctac cgatgatgag attaaaaaag
      421 cctaccggaa attggcgatg aaataccatc ccgaccgcaa tcctgacaat aaagaggcgg
      481 aagagaagtt taaagaagta caaaaggcgt atgaaacttt gtccgacaag gaaaagcgcg
      541 ctatgtacga ccagtatggt catgcggcgt ttgaaggcgg cggacagggg ggcttcggag
      601 ggtttggcgg atttggcggt gcgcagggtt ttgactttgg ggatattttc agccaaatgt
      661 ttggaggcgg ttcggggcgc gcccagcctg attatcaggg tgaggacgtt caagtcggta
      721 tcgaaatcac gcttgaagaa gccgcaaaag gtgtgaagaa acgcatcaat attccgactt
      781 atgaagcgtg tgatgtctgt aacggcagtg gcgcgaaacc ggggacatcc ccggaaacct
      841 gcccgacttg caaaggttcg ggtacggtgc acatccagca ggcgattttc cgtatgcagc
      901 agacttgtcc gacctgccac ggtgcgggca aacacattaa agaaccttgc gtcaaatgcc
      961 gtggcgcggg gcggaataag gcggtcaaga cggtggaagt caatattccc gccggtatcg
     1021 atgacgggca gcgtatccgt ttgagcggcg aaggcgggcc gggtatgcac ggtgcgcctg
     1081 ccggcgactt gtatgtaacc gtccgcattc gggcgcataa gattttccaa cgcgacggtc
     1141 tggacttgca ttgcgaactg ccgatcagtt ttgccacggc tgctttgggc ggggagttgg
     1201 aagtgccgac cttggacgga aaggtcaagc tcaccgtccc caaagaaacc caaaccggca
     1261 ggaggatgcg tgtgaaaggt aagggtgtca aatcgttgcg cagcagcgcg accggcgatt
     1321 tgtactgcca tattgttgtc gaaacgcctg tcaatttgac tgaccgtcaa aaagagcttt
     1381 tggaagagtt tgagcggatt tctaccggct tggaaaacca aacaccgcgc aagaaatcgt
     1441 ttttagacaa gctgcgcgat ttgtttgatt gattttaagg ttcggaaaca agcagccgta
     1501 tcggggaatc tccttgatac ggctgttttt atttgtttaa aaatagtttt tattttcaat
     1561 ggggtatgag gcagggtggg ataactgttt ttaactgttc tttttaaaac ttgacatcat
     1621 ggcgtgatgc caacaatatg tgaacgtctg ttgtcaaagg aagaataatg aataaatctt
     1681 tatccagttc ggtagaagaa taccgcgagc tgacgctccg aggcatgata ctcggtgcat
     1741 tgatcactgt aatttttact gcgtccaatg tttacctcgg tttgaaagtc gggctgacct
     1801 ttgcctcgtc gattccggcg gcggtgattt cgatggcggt tttaaagttt ttcaaaggca
     1861 gcaatatttt ggaaaacaac atggtgcaga cccaagcttc ggcggcgggt acgctttcga
     1921 ccatcatctt cgtcctgccc ggtttgctga tggcgggcta ctggagcggt ttcccgttct
     1981 ggcagacgac gcttttatgt attgccggcg ggattttggg ggtgattttc accattccgc
     2041 tgcgttacgc gatggtggtg aaaagcgatt tgccttatcc ggaaggtgtg gcggctgctg
     2101 aaattttgaa agtgggcggt catgaagaag gggataaccg tcagggcggc agcggcatca
     2161 aagagctggc ggccggcggt gcgttggcgg gattgatgag cttttgcgcc ggaggtctgc
     2221 gcgtgattgc cgacagcgcg agttattggt ttaaaagcgg tacggcgatt ttccagctgc
     2281 cgatgggctt ttcactggca ttgttgggcg cgggctattt ggtcggactg acgggcggta
     2341 tcgccatcct gttgggcatt tcgattgctt ggggcattgc cgtgccgtat ttctcctcac
     2401 acattccgca accttccgat atggaaatgg cggcgtttgc gatgaagctg tggaaggaga
     2461 aagtgcgttt tatcggtgcg gggactattg gcattgcggc ggtttggacg ctgttgatgc
     2521 tgctcaagcc gatggtggaa ggcatgaaga tgtcgttcaa gagttttggc ggcggtgcgc
     2581 ccgctgcgga acgcgccgaa caagatttgt cgcctaaagc catgattttt tgggtgctgg
     2641 cgatgatgtt tgttttaggc gtgtcgtttt accactttat cggcgattcg cacattacgg
     2701 gcggcatggc ttggcttttg gtggtcgttt gcacgctttt ggcttccgtc atcggctttt
     2761 tggtcgccgc cgcctgcggt tatatggcag gtttggtcgg ctcgtcttcc agcccgattt
     2821 ccggcgtggg catcgtgtcc gtcgtcgtta tttcactggt tttgctgctg gtaggcgaat
     2881 ccggaggttt gttggcggat gaggctaacc gcaaattttt gctggcactg actttgtttt
     2941 gcggctcggc agtaatctgc gtggcttcga tttccaatga caacctgcaa gacttgaaaa
     3001 ccggctacct gctcaaagcc acgccttggc ggcagcaagt cgccctgatt atcggctgta
     3061 tcgttggtgc gctggttatt tcgcccgtgt tggaactgct ttacgaagcc tacggcttta
     3121 ccggcgcaat gccgcgcgaa ggcatggata cggcgcaggc tttggcagct cctcaagcga
     3181 ctttgatgac gaccatcgcg tcgggcattt tcgcccacaa cctcgaatgg gtctacatct
     3241 ttaccggcat cgcgatcggc gcggtattaa tcgtcgtcga tttggtgttg aaaaaatcat
     3301 caggcggcaa acttgccctg cccgtccttg cggtcggtat gggtatttat ctgccgccgt
     3361 ccgtcaatat gcccatcgtg gcaggcgcgg tgttggcggc ggtgttgaaa cacatcatcg
     3421 gtaaaaaagc ggaaaaccgc gaaggccgtc tgaaaaacgc cgagcgtatc ggaaccttgt
     3481 tctccgccgg cctgattgtc ggtgaaagcc tgatcggtgt gattatggcg tttattattg
     3541 ccttctccgt gaccaacggc ggctcggatg cgccgcttgc gttgaatctg caaaactggg
     3601 atgccgccgc ttcttggttg ggtttggcgt tcttcgttac cgggatgttt ttctttgcac
     3661 agcgcgtact gaaggcgggc aagtaggctg tcgg
//
LOCUS       DAWHXZ010000188         3502 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000188 DAWHXZ010000000
VERSION     DAWHXZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.188"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            386..1195
                     /locus_tag="WIE35_001765"
     CDS             386..1195
                     /locus_tag="WIE35_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ4154647.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEYEAYEDKRHIPENPYFHEFYYIKKGENPAIITHRNYHRYGENDY
                     STSVGSCINGFTVRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
     gene            1212..>3502
                     /locus_tag="WIE35_001766"
     CDS             1212..>3502
                     /locus_tag="WIE35_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ4154648.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGR
                     LPNTSEIERSYYKAVTENGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNAQA
                     VNNPNGSEWDNRKQLISALDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVAKREWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKET
                     KKRNGKYHVYDPLALDLDGDGIETVATKGFAGSLFDHTNNGIRTATGWVSADDGLLVR
                     DLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINAADAAFQTLRVW
                     QDLNQDGISQANELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGSYTKTDGTTAKM
                     GDLLLAADNLHSRFKDKVELTAEQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSA
                     AETKEAQLALLDNLIHKWAETDSNWGKKSPMRLSIDWTQTANEGIALTPSQVAQLKKN
                     ALVSLSDKAKAAIDAARDRIAVLDAYTGQDSSTLYYMSEEDALNIVKVTNDTYDHLAK
                     NIYQNLLFQTRLQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEML
                     AYGELRSWYEGRRLMADYVEEAKKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNV
                     GFGHNKNVSLYGNDGNDTLIGGAG"
BASE COUNT         1118 a          664 c          769 g          951 t
ORIGIN      
        1 ttacagtcga tgaaccacca ttttgcttta tttcagcggg cttgagatgt gtataagaat
       61 attgttttga ataaatttaa agaaaatgat aatcgttatt gacgattttt aaaggaaagc
      121 gtagagtgcc aattctatga agcaatacgg taagtaacaa tgaaaatatc tactgcttgg
      181 gtatagagca tatttcacaa cccgtaacta ttcttgcgga aacagagaaa aaagtttctc
      241 ttctatcttg gataaatata tttaccctca gtttagttaa gtattggaat ttatacctaa
      301 gtagtaaaag ttagtaaatt atttttaact aaagagttag tatctaccat aatatattct
      361 ttaactaatt tctaagcttg aaattatgag accatatgct actaccattt atcaactttt
      421 tattttgttt attgggagtg tttttactat gacctcatgt gaacctgtga atgaaaagac
      481 agatcaaaaa gcagtaagtg cgcaacaggc taaagaacaa accagtttca acaatcccga
      541 gccaatgaca ggatttgaac atacggttac atttgatttt cagggcacca aaatggttat
      601 cccctatggc tatcttgcac ggtatacgca agacaatgcc acaaaatggc tttctgacac
      661 gcccgggcag gatgcttact ccattaattt gatagagatt agcgtctatt acaaaaaaac
      721 cgaccaaggc tgggttcttg agccatacaa ccagcaaaac aaagcacact ttatccaatt
      781 tctacgcgac ggtttggata gcgtggacga tattgttatc cgaaaagatg cgtgtagttt
      841 aagcacgact atgggagaaa gattgcttac ttacggggtt aaaaaaatgc catctgccta
      901 tcctgaatat gaagcttatg aagataaaag acatattcct gaaaatccat attttcatga
      961 attttactat attaaaaaag gagaaaatcc ggcgattatt actcatcgga actatcatag
     1021 gtatggagag aacgattaca gcactagcgt aggttcctgt attaacggtt tcacggtacg
     1081 gtattacccg tttattcggg aaaagcagca gctcacacag caggagttgg taggttatca
     1141 ccaacaagta gagcaattgg tacagagttt tgtaaacaat ccaagtaaaa aataatttaa
     1201 aggatcttat tatgaatgag ggtgaagttg ttttaacacc agaacaaatc caaaccttgc
     1261 gtggttatgc tttccgtggc gatacctatg gcggttggcg ttatttggct aatttgggtg
     1321 accgttatgc ggatgatgct gctgcaattg tcggtaagga tgcaaactta aatggtttga
     1381 atttatggat gaaaaaaggt gtggaaaacc tatgggatga tacggtcggt aaaaagaccc
     1441 gtttagagaa atttgatcgg gttgcattgc aacatttcag ccaatatgta gatctaatta
     1501 atgaaaataa tggtagatta cctaacacta gtgaaattga gagaagttac tataaagccg
     1561 ttaccgaaaa tggtgtttct tctagtgcag ctattgattt agttattaat cgctcacttc
     1621 cggatatggc agatggttat tgggcattag gtttggggat agaagccgaa cgtatccaca
     1681 atgcgcaagc agtaaataat ccgaacggta gcgaatggga taatagaaag cagttaatat
     1741 ctgctttaga taaaggattt gatggatctt ttaaagagaa gcattttact tttttacaat
     1801 ctgtgataat ggatgtaaca aagttaggtg ttgaatatac aatagatggt tggcaaaaaa
     1861 ttggaggttg gggtaatggg ataatcaatg atttatataa aagtgttgca aaaagagagt
     1921 ggactggaat atttgagatc gttaataata acatcaagca atttagagat ctgttcccaa
     1981 atccggaagg ctggatcgat gatggtcacc aatgtttcgc tccttgggtt aaagaaacta
     2041 aaaaacgcaa tggcaaatat catgtctacg accccctcgc cctagatttg gacggagacg
     2101 gtatagaaac cgttgccacc aaaggctttg caggcagctt atttgatcac accaacaacg
     2161 gtatccgcac cgccaccggt tgggtttctg ccgatgacgg tttactcgtc cgcgatttga
     2221 acggcaacgg catcatcgac aacggtgcgg aactcttcgg cgacaacacc aaactggcag
     2281 acggttcttt tgccaaacac ggctacgcgg ctttggccga attggattca aacggcgaca
     2341 acatcatcaa cgcggcagac gccgcattcc aaaccctgcg tgtatggcag gatctcaatc
     2401 aggacggcat ttcccaagct aatgaattgc gtacccttga agaattgggt attcaatctt
     2461 tggatctcgc ctataaagat gtaaataaaa atctcggtaa cggtaacact ttggctcagc
     2521 aaggcagcta taccaaaaca gacggtacaa ccgcaaaaat gggggattta cttttagcag
     2581 ccgacaatct gcacagccgc ttcaaagaca aagtggaact cactgccgaa caggcaaaag
     2641 ccgccaatct tgcgggcatt ggtcgtctgc gcgatttgcg cgaagctgcc gcattgtccg
     2701 gcgatttggc caatatgctg aaagcttatt ctgccgccga aactaaagaa gcacagttgg
     2761 cattgttaga taatttgatt cacaaatggg cggaaaccga ttcgaactgg ggcaaaaaat
     2821 cgccaatgcg actttcaatc gattggacgc aaacggctaa tgaaggtatt gcactgacac
     2881 catcccaagt agcacaacta aaaaagaacg ctttagtttc cctttctgat aaagctaaag
     2941 cagctattga cgccgcccgc gaccgcattg ccgtgcttga tgcctacacg gggcaggatt
     3001 ccagcacact ctattacatg agcgaagaag acgcgcttaa tatcgtcaaa gtaaccaacg
     3061 atacatacga ccatctcgcc aaaaacatct accaaaacct gttgttccaa acccgtttgc
     3121 agccatattt gaatcaaatc agtttcaaaa tggaaaatga tacgttcact ttggatttta
     3181 gtggtcttgt tcaagcattt aaccatgtca aagaaactaa tccgcaaaaa gcttttgtgg
     3241 atttggccga gatgcttgca tatggcgaac ttcgttcttg gtatgaaggc cgaagactaa
     3301 tggccgatta tgtggaggag gcaaaaaaag caggtaaatt tgaagattac cagaaagtgt
     3361 tgggtcagga gaccgttgca ttattagcta aaacatcggg tacgcaagca gatgatatcc
     3421 tgcaaaatgt aggctttggt cataataaaa atgtttcttt atatggtaat gacggcaacg
     3481 acactctgat cggcggtgcc gg
//
LOCUS       DAWHXZ010000189         3484 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000189 DAWHXZ010000000
VERSION     DAWHXZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.189"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            205..1677
                     /gene="pyk"
                     /locus_tag="WIE35_001767"
     CDS             205..1677
                     /gene="pyk"
                     /locus_tag="WIE35_001767"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ4154649.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            1985..3385
                     /locus_tag="WIE35_001768"
     CDS             1985..3385
                     /locus_tag="WIE35_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ4154650.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWS
                     TMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFE
                     KEKTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMN
                     SLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARF
                     KNHADIIGLQYTYKFK"
BASE COUNT          900 a         1049 c          889 g          646 t
ORIGIN      
        1 taaatttagc ctaagtcata aaaccgtgta attttgcgcc aaaaaaggcg gtttgcaggt
       61 aaaatatcaa actattaatt taaatcatat ttttacagaa tattccgccg cgttcatcaa
      121 atggacatca aaccggttcc aaatttctgt aattttgtaa caaaataccg caaaacaccc
      181 gattgagacc aaaaggactt tcatatgaac cagacaagcc gcgatctgac ccgcatcagc
      241 cacaacacta aaatcgtcgc cacccttggg ccgggcagca acaacgtaga actgttggaa
      301 gatatgatcc gcgtcggcgg cctgaacgtc gtccgcttca acttcagcca cggcacgccc
      361 gaattccatc aggaaaacgc ccgcatcgtg cgcgaggcgg caaaacgcgc cggacaggaa
      421 atcgccatca ttgccgacct gcaagggccg aaaatccgcg tgggcaaaat cgccggcggc
      481 ggcattgaat tgaacaaagg cgaaacgctg gtactggatg ccgcgctcga aggcgaaggc
      541 acgcgcgagg cggtcggttt ggactaccgc gacctgcccg acgacgttgc cgcaggcgac
      601 gtattgtggc tggacgacgg cctgctgacc ctgaccgtgg aatccgtcga aggcagcagg
      661 attatcacaa gggtggaaaa cagccatgtc ctgaaaagca acaaaggcat caacaaacgc
      721 ggcggcgggc tgtccgcagg cgcgttgacc gaaaaagact tccgcgacct gaaaaccgcg
      781 attgccatcg gttgcgacta cctcgccatc agctttgtga aatctgccga agatttgcac
      841 atcgcgcgcg ccaaagtcga agaagaaatg aagggcagca ctgccgtgcg ccccggtttg
      901 gtttccaaaa tcgaacgcgt agaagcgatt gaaaaccttg atgaaatcat cctcgccggc
      961 gacggcatta tggttgcgcg cggcgacttg gcggtcgaag tcggacacgc cgccgtcccc
     1021 gccctgcaaa aacggatgat ccgccgcgcc cgcgagttgc gccgcttcag cattacggcg
     1081 acgcaaatga tggaatcgat gattaccaac cccgtgccga cccgcgcgga agtcagcgat
     1141 gtggcaaacg cggtattgga cggtaccgat gcggtgatgt gttccgccga aaccgccgtc
     1201 ggcgcgtatc cgtttgaaac cgtcagccaa atggcaatta tctgcgcggc tgcggaaaaa
     1261 gagcaggatt cgctcaacgg cgttgccgaa caggcggagt atcccgaagc ggtcagcacc
     1321 aacctggcgg ttgccggcgg cgcggtcagc gtggcgcgcg cggttcacgc caaagcgatt
     1381 gtcgccctga ccgaaagcgg ttcgaccgcc tttgaaatca gccgccacaa catcaccctg
     1441 ccgattttcg cgctgacccc gagcgtttcc gcccaacgcc gtatggcgat gtaccgcggc
     1501 gtgcgcccgc tgattttggc aaccagtacc gaccacgaca cggcgttgaa cgaagtcgaa
     1561 accatgctgg tggaacacaa catcctgcat tccggcgacc agtacatcat caccagcggt
     1621 tcgcaaatgc gcgaatccgg ttcgaccaac acactggaag tgctgcgcgt caaataatcc
     1681 gccctgagtg gaaaatgccg tctgaagccg atgcccgagg cttcagacgg cattttttgc
     1741 ggcggcgcgg cggttcgggc aaactgaaaa aattgtgcaa atccggcaac atcggataaa
     1801 attgagtacc tatactaaag cgaaacaagg catttccgac tgcctttttt atagtggatt
     1861 aaatttaaac cagtacggcg ttgccttgcc gtactatctg tactgtctgc ggcttcgtcg
     1921 ccttgtcctg atttttgtta atccactata tttatccacc gtccgccttt ttacggaaac
     1981 cgaaatgacc ccttccgcac tgaaaaaaac cgtcctactg ctcggcactg cctttgccgc
     2041 cgcatccgca caagcctccg gctaccactt cggcacacag tcggtcaacg cgcaaagcac
     2101 ggcaaatgcc gccgccgcag aagccgccga cgcatcgacc atcttctaca accctgccgg
     2161 cctgaccaaa ctcgacagca gccagatttc cgtcaacgcc aacatcgtgc tgcccagcat
     2221 tcattatgag gcggattccg ccaccgactt taccgggctt cccgtccaag gttcgaaaag
     2281 cggcaaaatc accaaaacca cggtcgcgcc ccacatctac ggcgcataca aagtcaacga
     2341 caatctgacc gtgggcttgg gcgtgtacgt ccccttcggc tctgccaccg aatacgaaaa
     2401 agattccgtg ttgcgccaca acatcaacaa actcggtctg accagcatcg ccgtcgaacc
     2461 tgtcgccgca tggaaactca acgaccgcca ttccttcggc gcaggcatca tcgcccaaca
     2521 tacttccgcc gaactgcgca aatatgccga ctgggggatt aagagtaaag cagagatatt
     2581 gacggcaaaa ccgcccaaac ctaacggtgt agccgaagct gcaaaaattc aggccgacgg
     2641 acacgccgat gtcaaaggca gcgattgggg cttcggctac caactggcgt ggatgtggga
     2701 catcaacgac cgcgcgcgcg tgggcgtgaa ctaccgttcc aaagtctcgc acacgctcaa
     2761 aggcgatgcc gaatgggcgg cagacggcgc ggcggcgaaa gcaatgtgga gtacgatgct
     2821 tgcagcaaac ggctacacgg cgaatgaaaa agcccgcgtt aaaatcgtta cgcctgagtc
     2881 tttgtccgta cacggtatgt acaaagtgtc cgataaagcc gacctgttcg gcgacgtaac
     2941 ttggacgcgc cacagccgct tcgataaggc ggaactggtt tttgaaaaag aaaaaaccat
     3001 cgtcaacggc aaatccgacc gcaccaccat cacccccaac tggcgcaaca cctacaaagt
     3061 cggcttcggc ggttcttatc aaatcagcga accgctgcaa ctgcgcgccg gcatcgcttt
     3121 tgacaaatcg cccgtccgca acgccgacta ccgcatgaac agcctgcccg acggcaaccg
     3181 catctggttc tccgccggca tgaaatacca tatcggcaaa aaccacgtcg tcgatgccgc
     3241 ctacacccac atccacatca acgacaccag ctaccgcacg gcgaaggcaa gcggcaacga
     3301 tgtggacagc aaaggcgcgt cttccgcacg tttcaaaaac cacgccgaca tcatcggcct
     3361 gcaatacacc tacaaattca aataaacggc ccgccgtttg attgtagaaa tgccgtctga
     3421 aacagatttc cgtttcagac ggcattgttt taggcgcgat ttatagtgga ttaacaaaaa
     3481 tcag
//
LOCUS       DAWHXZ010000190         3477 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000190 DAWHXZ010000000
VERSION     DAWHXZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.190"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     regulatory      64..167
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            234..1460
                     /gene="cytX"
                     /locus_tag="WIE35_001769"
     CDS             234..1460
                     /gene="cytX"
                     /locus_tag="WIE35_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ4154651.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARRTGGLKTVSMLLMLLAV
                     LWLSVEVFASSGTNAAPAVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATL
                     TATLAYTLTSCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDA
                     YSAGVSANNISAKLSETPIAVAVAVVGTLLAVLLPVTEYENFLLLIGSVFAPMAAVLI
                     ADFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVL
                     VRLFFKKTQSLQRNPS"
     gene            1457..2557
                     /locus_tag="WIE35_001770"
     CDS             1457..2557
                     /locus_tag="WIE35_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ4154652.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVIKLGRQSIPLWRGIRCRLKTPAMMQENGSLIVWHGQDKPL
                     SNEFVRHLKRGGVADDEIVRWRTDDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTVWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSRKRRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            2578..3195
                     /gene="thiE"
                     /locus_tag="WIE35_001771"
     CDS             2578..3195
                     /gene="thiE"
                     /locus_tag="WIE35_001771"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ4154653.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIARCVAACQGSHTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYIASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT          721 a         1088 c          947 g          721 t
ORIGIN      
        1 atgcgccgtg ccgccgaaaa tgtaagaatt ttgccaacgc acgttgcttt gtgtaaactt
       61 aaagctttct tgtcggagtg ccgccgccgg gcggctgaga ttgcgaaagc agaatccgta
      121 gaacctgtcg gggtaatgcc tgcgtaggaa acaaaccgtc aaatgcctta tcgggcttcc
      181 gttccctttt ccgcacttcc ccgcccacct tccttctttt taaaggacgt catatgtcgg
      241 gcaatgcctc ctctccttca tcttccgccg ccatcgggct gatttggttc ggcgcggcgg
      301 tatcgattgc cgaaatcagc acgggtacgc tgcttgcgcc tttgggctgg caacgcggtc
      361 tggcggctct acttttgggt catgccgtcg gcggcgcgct gttttttgcg gcggcgtata
      421 tcggcgcact gaccggacgc agctcgatgg aaagcgtgcg cctgtcgttc ggcaaacgcg
      481 gttcagtgct gttttccgtg gcgaatatgc tgcaactggc cggctggacg gcggtgatga
      541 tttacgccgg cgcaacggtc agctccgctt tgggcaaagt gttgtgggac ggcgaatctt
      601 ttgtctggtg ggcattggca aacggcgcgc tgattgtgct gtggctggtt tttggcgcac
      661 gcagaacggg cgggctgaaa accgtttcga tgctgctgat gctgcttgcc gtgttgtggt
      721 tgagcgtcga agtgttcgct tcgtccggca caaacgcagc ccccgccgtt tcagacggca
      781 tgagcttcgg aacggcagtc gaactgtccg ccgtcatgcc gctttcctgg ctgccgcttg
      841 ccgccgacta cacgcgccac gctcgccgtc cgtttgcggc aaccctgacg gcaacgctcg
      901 cctacacgct gacgagctgc tggatgtatg ccttgggttt ggcagcggct ctgtttaccg
      961 gagaaaccga tgtggcaaaa atcctgctgg gcgcaggttt gggtgcggca ggcattttgg
     1021 cggtcgtcct gtcgaccgtt accaccactt ttctcgatgc ctactccgcc ggcgtaagtg
     1081 ccaacaatat ttccgccaaa ctttcggaaa cacccatcgc cgttgccgtc gccgttgtcg
     1141 gcacactgct tgccgtcctc ctgcccgtta ccgaatatga aaacttcctg ctgcttatcg
     1201 gctcggtatt tgcgccgatg gcggcggttt tgattgccga ctttttcgtc ttgaaacggc
     1261 gtgaggagat tgaaggcttt gactttgccg gactggttct gtggctggcg ggcttcatcc
     1321 tctaccgctt cctgctctcg tccggctggg aaagcagcat cggtctgacc gcccccgtaa
     1381 tgtctgccgt tgccattgcc accgtactgg tgcgcctttt ctttaaaaaa acccaatctt
     1441 tacaaaggaa cccgtcatga cccgtatcgc catcctcggc ggcggcctct cgggaaggct
     1501 gaccgcactg cagcttgcag aacaaggtta tcagattgca cttttcgata aaggctgccg
     1561 ccggggcgaa cacgccgccg cctatgttgc cgccgccatg ctcgcgcctg cggcggaagc
     1621 ggtcgaagcc acgcctgaag tcatcaaact gggcaggcag agcatcccgc tttggcgcgg
     1681 catccgatgc cgtctgaaaa cacctgccat gatgcaggaa aacggcagcc tgattgtgtg
     1741 gcacggacag gacaaacctt tatccaacga gttcgtccgc catctcaaac gcggcggcgt
     1801 agcggatgac gaaatcgtcc gttggcgcac cgacgacatc gccgaacgcg aaccgcaact
     1861 cggcggacgt ttttcagacg gcatctacct gccgaccgaa ggccagctcg acgggcggca
     1921 aatattgtct gcacttgccg acgctttgga cgaactgaac gtcccctgcc attgggaaca
     1981 cgaatgtgcc cccgaagacc tgcaagccca atacgactgg ctgatcgact gccgcggcta
     2041 cggcgcaaaa accgtgtgga accaatcccc cgaacacacc agcaccctgc gcggcatacg
     2101 cggcgaagtg gcgcgggttt acacacccga aatcacgctc aaccgccccg tgcgcctgct
     2161 gcacccgcgt tatccgctct acatcgcccc gaaagaaaac cacgtcttcg tcatcggcgc
     2221 gacccaaatc gaaagcgaaa gccaagcccc cgccagcgtg cgttcagggt tggaactctt
     2281 gtccgcactc tatgccgtcc accccgcctt cggcgaagcc gacatcctcg aaatcgccac
     2341 cggcctgcgc cccacgctca accaccacaa ccccgaaatc cgctacagcc gcaaacgccg
     2401 cctcatcgaa atcaacggcc ttttccgcca cggtttcatg atctcccccg ccgtaaccgc
     2461 cgccgccgtc agattggcag tggcactgtt tgacggaaaa gacgcgcccg aacgtgacga
     2521 agaaagcggt ttggcgtata tccgaagaca agattaaagc cgtctgaaag gaaccccatg
     2581 accttcccgc cactcaaatc cccgctcaaa ttctacgccg tcgtccccac cgccgattgg
     2641 gtggggcgca tggtcaaagc aggtgccgac acggtgcaac tgcgctgcaa ggccctgcac
     2701 ggcgatgaat tgaaacgcga aatcgcccgc tgtgtcgccg cctgtcaggg cagccatacg
     2761 cagcttttca tcaatgacca ctggcgcgag gcaatcgaag caggcgcgta cggcgtgcat
     2821 ctcggacaag aagacatgga caccgccgac cttgccgcca tcgccgccgc cggtttgcgc
     2881 ttgggtttga gtacgcactc cgttgccgaa ctcgaccgcg ccctgtccgt acaccctagc
     2941 tacatcgcca gcggcgcgat tttcccgacc acgaccaagc aaatgcccac cgccccgcaa
     3001 ggcttggaca aactgtgcga atacgtcaaa caggcaggcg gcacgcccgt cgtcgccatt
     3061 ggcggcatcg atttgaacaa cgcccgagcc gtactcgcca ccggcgtttc ctcactcgcc
     3121 gccgtccgcg ccgtaaccga agcggcaaat cccgaagcgg tggttaaagc gtttcaggct
     3181 ttgtgggatg gataaaaccg aaagaagaaa attcaattgc cgtgtaggca aaacttagcc
     3241 cgttatcgca aacatactta actttaaatg tggcatatca tcaaattccg tcattcccgc
     3301 gcaagcggga atccgcctta aaacttgaga aaccatcatt tgaaaaacag tttccgaatt
     3361 tcaaaaatgg attcccgcct gcgcgggaat gacggcaacc ggccggttgc gtatcaaaaa
     3421 ataaagtaat tcggctagat atagtggatt aacaaaaatc aggacaaggc gacgaag
//
LOCUS       DAWHXZ010000191         3395 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000191 DAWHXZ010000000
VERSION     DAWHXZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.191"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(231..1295)
                     /locus_tag="WIE35_001772"
     CDS             complement(231..1295)
                     /locus_tag="WIE35_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ4154654.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKAKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAGH
                     QCVANRFTINHTADKLLDAI"
     gene            complement(1296..2054)
                     /locus_tag="WIE35_001773"
     CDS             complement(1296..2054)
                     /locus_tag="WIE35_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ4154655.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2129..2204)
                     /locus_tag="WIE35_001774"
     tRNA            complement(2129..2204)
                     /locus_tag="WIE35_001774"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2169..2171),aa:Ile,seq:cat)
     gene            complement(2258..>3395)
                     /locus_tag="WIE35_001775"
     CDS             complement(2258..>3395)
                     /locus_tag="WIE35_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEZ4154656.1"
                     /translation="VDWHERFPELEAQLGAPIEDYAPPKHWTRKQLRSMGRVSYLCVD
                     AAEQALTDAGLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTGTSRNFSANTYVR
                     MMPHTTAANIGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFF
                     SSEVYVFDSLYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAII
                     YAELVGYGANSDAYHISTPRPDAQGAILAFQTALQHAGLAPEDIGWINLHGTGTHHND
                     SMESRAVAAVFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRQSNPEGKLPPRLW
                     DGQNDPDLPAINLTGSGSRWETEKHIAASSSFAFGGSNCVLIIG"
BASE COUNT          759 a          834 c          907 g          895 t
ORIGIN      
        1 gtactggttt ttgttagtct actatacatt gtaacaaagc acttgatgcg gcagcacaag
       61 ccggggaaga cgctgcaaaa ctttgccaat aggctgcgta atatcggggg atattgggat
      121 tttcttattt ttaaggatgg attcggggtg tttctttcca atgaacgaat ataatttggc
      181 ggtttgttgc atcaaaggct ttcagacggc atatgccgtc tgaaagcagt ttatattgca
      241 tccaataatt tgtcggcggt atggttgatg gtaaaacggt tggcgacgca ttgatgtcct
      301 gccgttgcca gtgcgttttt cgtttcaggg ttcaagacga ggcgttcgat ttcgtccgcc
      361 aaagattcag gtgtcagcct gtccagcaga atccccgatt gatgatgttc gataatttcc
      421 ttttgcgccc ccaaagtatt ggaaatcacc gccgttcggc agtacatcgc ctcgcataat
      481 gacaaaccga atgcctccgg gacgaggctg ggcaaaacca cgacatcgct ttggcggtaa
      541 aacgaagcaa gtttttcggt aaacccttca aaagaaacaa atggttctgc tcctgaagca
      601 gatacgtccc gcttcaggcg gcacatataa tccggatgtc cgtcccctgc caatttgagc
      661 ctgatttgag gatatttccg atgcagtatc acacaggctt caatcaggtt ttccaatcct
      721 ttttctggac tgatcctgcc ggcgtaggcg acggtaaaaa aacggctgtc gggtttttct
      781 tgagagggag ggaaacggcc ggtatcgata ccgttatgaa caatccggta ttttgcttta
      841 aagggattgt cggcggtttg cacatcgtaa accagacggg aaacgcaaat aaagcggtct
      901 gtgtttttct gtatcaggcg gtggtaaaaa tcggttttgt tggcgacgac attatgtttg
      961 acaaatatca ggcgcacccc ggtcagtttt ttcaaaagta tggataaggc ggcaattttg
     1021 ccggtgtgta tcatcaggtg ggaaatgcgg tttttccgga taaagcgggt aagcgcgcag
     1081 agcgaaaaca gcccgttgag cgtgtgaagg cgcgttgtga aaacggaaga aacttcggaa
     1141 aaacgtctgt gcatcaactc attattttta ttgacggcgg taaacatcgt gcaaccccga
     1201 agccccaatg cttttgaaac atcatagaca tactgttcgc ccccgcccca aatcttggcc
     1261 gatacgatat ttaatatcct gaattctttt tccatttaaa attttccgcc agatttgtac
     1321 agataatata gtttgacata tttaatcatc gtgtaatagc tgtggttgac ggacataatc
     1381 caacccattt ttccatcgag aaaccctttg ttcaggatat aaattttgaa aaaaccccaa
     1441 atcgggcgga ggataatgtc cctaacgaaa cgcacgggct ttccctgctc tcggtatttc
     1501 tcggctgaga tggaagtgta tttgttgaat ttgttgaaat attgttccca gttgtcgtat
     1561 gtgtaatggt acataaaatg cttcagacgg cgtttggggt agggggtttg tacggtttcg
     1621 tgtactttgc cttgcaccga actgtctttt ttcggcatca gacggcatac gctgtcggga
     1681 cgcatcgcgc cgtgtgtggc gggatggttg gggaaaaggt tgcggcgttc gacaaaatag
     1741 gcggcataat cgccggtttg gacaattttt gagatttcat cagatagttc cggcgtgcag
     1801 cgttcgtctg catcaatcag gaaaacccat tctccgcctg cctgttcgat ggcaaatgtt
     1861 ttttgcgctc cgaaatcccc gttcaaatgc cgtctgaaga cttttgcacc caaaccctcg
     1921 gcaatttcgg cagtattgtc ggtgctgtgg tcgtcaataa cgataacttc tttatcgaaa
     1981 cggcaacttt caatacattc ccgaatgtgg tttgcttcgt ttttggcaac aatcaatacg
     2041 ctgacttttt tcattatcaa ggtttctcaa agcattttgg tttcacaggg cggcaaaaaa
     2101 gcccaaacat acgtccgggc ttgggaattg gtgggtccgg tggggttcga acccacgacc
     2161 aagggattat gagtcccctg ctctaacccc tgagctacag acccgtcaag agtaggcgcg
     2221 tattgtatag catagcggtg ggattgacaa acttatttca tccgatgatt aagacgcagt
     2281 tgcttcctcc gaaggcaaac gacgagctgg cggcaatgtg tttttcggtt tcccagcggc
     2341 tgccgctgcc ggtcaggttg atggcgggca ggtcggggtc gttctgcccg tcccaaagcc
     2401 ggggcggaag tttcccttcg ggattgcttt gccggtcggc aatgccccac gcgaacgcgg
     2461 cttcgattgc gcccgccgcg cccagcgtgt gtccggtttg cggcttggtg gacgtgcagg
     2521 gcgtattgtt gccgaaaacc gctgcaacgg cacggctttc catactgtcg ttgtggtgcg
     2581 tcccggtgcc gtgcagatta atccagccga tgtcttcggg cgcaagacct gcgtgttgca
     2641 atgccgtctg aaaggcaagg attgcgcctt gcgcgtcggg gcggggcgtg gaaatatggt
     2701 aggcatcgct gttggctccg tagccgacga gttcggcgta aattatcgca ccgcgccgtt
     2761 tggcgtgttc caattcttcc agcacgaaaa tccccgcgcc ttcgccgatg accagcccgt
     2821 cgcggttcgc gtcgtatggg cgcggggttt tttccggttc gccgttgcgg cggctggcgg
     2881 cataaagcga gtcgaaaaca tacacttcgg acgagaaaaa ttcttcgcct ccgcccgcca
     2941 gcatcatatc ggtcagaccg tatttgatgg cttcgtaggc ataacctatg ccttggctgc
     3001 cggacgaaca cgcgctcgat gtcgggatga tgcgtccttt cagcccgaaa aagatgccga
     3061 tattggcggc ggtggtgtgc ggcatcatac gcacataggt gttggcactg aagttgcgcg
     3121 acgtgccggt cagcaacaat tcgcccacat cgccgatgtc tttggtgctg ccgctggaag
     3181 agccgcaggc aacgcccatc cgtccgtcgg taatgctttc gtccccgagc aaaccggcat
     3241 ccgtcaaagc ctgctccgcc gcatcgacgc acaaatacga cacacgcccc atacttctga
     3301 gctgcttgcg cgtccaatgt ttcggcggcg cgtaatcctc aatcggcgca cccagttgcg
     3361 cttccaattc ggggaaacgt tcgtgccaat ccact
//
LOCUS       DAWHXZ010000192         3339 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000192 DAWHXZ010000000
VERSION     DAWHXZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.192"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(63..1256)
                     /locus_tag="WIE35_001776"
     CDS             complement(63..1256)
                     /locus_tag="WIE35_001776"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinyldiaminopimelate
                     transaminase"
                     /protein_id="HEZ4154657.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALADALNTQMHKLWHISNIYTTRPAQELAQKLVKNSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATETIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVCSQGQKLQTALRSLGEQTGVFKQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLDDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            1660..2160
                     /locus_tag="WIE35_001777"
     CDS             1660..2160
                     /locus_tag="WIE35_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154658.1"
                     /translation="MNAMPQTAAQNETLHICSIIVDDARDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            2245..2799
                     /locus_tag="WIE35_001778"
     CDS             2245..2799
                     /locus_tag="WIE35_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154659.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            2813..3013
                     /locus_tag="WIE35_001779"
     CDS             2813..3013
                     /locus_tag="WIE35_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154660.1"
                     /translation="MPSEGLSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
BASE COUNT          809 a          964 c          854 g          712 t
ORIGIN      
        1 gcaaacaaaa ccgcttcgga acgttgaacc acgtttccga agcggttttt tgtttttgat
       61 gcctaggggc ggtttgcggc ggcaaattcc gtcagcacgt gttccaaacg ccgcaaacct
      121 tccgccgtat cctcatcgtc caacagcagc gaaggcgcga aacgcaatac gtccgcaccc
      181 gcaaccaaaa tcatcacgcc gtgtttcaag gcggcggcgg ttatttcgga tgcgcgtccg
      241 cgataaggcg cgtccaacac gcagccgagc agcagcccca tgccgcgaac ttgcttaaac
      301 acgcccgttt gttctcccaa agaccgcaat gccgtctgaa gtttctgccc ctgactgcag
      361 acgttgtgca aggtttccgg cgcgttgatg atgtcgaagg cgcggctgcc gaccgcacac
      421 gccatcgggt tgccgccgaa agtcgagcca tgcgtgcccg gttggaaggc ggcggcaatc
      481 gtttctgtcg ccagcatcgc gccgatcgga aagccgcagc ccaaggcttt tgccgaactc
      541 aaaatatcgg gaacaacgcc gtaatgttca taggcaaaca gcctgcccgt atgccccatc
      601 ccggtttgaa cttcgtccaa aatcaacaac gcattgtgcc ggtcgcacag acggcgcgcg
      661 gtttgcaaat attccgcagt ggcgggcagg atgccgcttt cgccctgtat cggctcgatg
      721 atgaccgcgc aggtctgttc gccgacggca gcttccagcg cggcaatatc gttgaacgga
      781 acgtgcgtaa tgccttgcgg caggggtgca taatccttgc tgtatttcgg ctgaccgccg
      841 acggacacgg taaacagcgt gcgtccgtgg aaactgttga tacaggcgac gatttcgctt
      901 tttcctccgc cgaaacggtc gcgcgcatat ttccttgcca gcttcaacgc cgcctcattc
      961 gcttccgcgc ccgagttgca gaaaaaaacc ttgtcggcaa aactgttctt caccaatttt
     1021 tgcgccaatt cctgcgccgg acgcgtcgta tagatattgg aaatgtgcca cagcttgtgc
     1081 atctgcgtgt ttaaagcatc ggcaagggca gggtggcagt gtcccagcgc attgacggcg
     1141 atgccgcctg aaaagtcaat atattcacgc ccttccgtat cccaaacgcg gctgcctgaa
     1201 gcgcgttcgg gaatcatcgg tgcaaaggcg aaattggggg tcagatagtt ttgcataatt
     1261 gctcctgatt gcggttttgc tcgggattat gcaccgtttt tcaaagcaga taaagggaaa
     1321 ttgccgacaa tatcgggcag cggtaaaaat aaaccgaaat attttcaatc ggatagatga
     1381 aaaatcaaaa aaatttcgat aaaatgcttg aggcgcggca gcgttttgat ttagaatctc
     1441 cccaatattt ttatcttcag acggcatttc atcatgcaag gcaaaaaatt tacaaccgct
     1501 tattgctatt ggtaccacac agacgtgcgg tacttttttg cgcttgcttg aaatccatcc
     1561 catacgcaaa acaggccgct cgaaaatggg gcggcttttt tgttaccttc acacaggtcg
     1621 cccgattcgg caacacacga cagaatcagg aaaatgacca tgaatgcaat gccccaaacc
     1681 gccgcacaaa acgaaaccct gcacatttgc agcattattg tcgatgatgc acgggatttt
     1741 tcccccatgc ttctcgatgc agggcgggac gacacactca tcagcgaatc gatgattccg
     1801 caaatccgca ctgttgccgc cctgatagcc gccgaacggc acgacttcag ccgcagcagc
     1861 cccgccgagt ttaccgatgc cgccgatttt tttgccgcac ggattctcgt attgggcgtg
     1921 cgacgcttcc atctggacgt atcgctgctt cagattttga aaaccgccaa cagccgcgcc
     1981 cgccgctttg ccgaaaaaca ccgcctgttt ttcacacccg cacaagccga attgagcctg
     2041 aacaaacaca aaaaccgccg cctgctgacc gtagaaaccg aacacgaagt cgaaaacaaa
     2101 ggcaacccag tcgccaacag cctcgcattt gtccgcaaat tgcacaccct gcccctgtaa
     2161 acatcacgat ttgccggcag aatgcttgcc aaaattcccg acggcgatac aataaaaccg
     2221 attttccacc caaacaggac acacatgaaa aaaatcatcg cctccgcgct tatcgcaaca
     2281 ttcgcactcg ccgcctgcca agacgacacg caggcgcggc tcgaacagca gcagaaacag
     2341 attgaagccc tgcaacagca gctcgcacag caggcagacg atacggttta ccaactgact
     2401 cccgaagcag tcaaagacac cattcctgcc gaagcacagg caaacggcaa caacgggcaa
     2461 cccgttaccg gtaaagacgg gcagcagtat atttacgacc aatcgacagg aagctggctg
     2521 ctgcaaagcc tggtcggcgc ggcggcaggc gcgtttatcg gcaacgcgct ggcaaacaaa
     2581 ttcacacggg caggcaacca agacagtccc gtcgcccgac gcgcgcgtgc agcctaccat
     2641 cagtccgcac atcccaatgc gcgcaccagc agggatttga acacgcgcag cctccgtgca
     2701 aaacaacagg cggcgcaggc gcagcgttac cgcccgacaa cgcgcccgcc cgccaattac
     2761 cgccgccccg ctatgcgcgg tttcggcagg aggcggtaaa cccggcgtgc caatgccgtc
     2821 tgaagggctt tcagacggca tttttgcatt tgttagggac attgttatgt tgccgtttga
     2881 ttttcagacg gcattttgtt tccaagcgtt tgatgtcggg atggcaattc tgatttacgt
     2941 gcgttggcag ggtggcattg tcatgccgcc gttgggtttt cagacggcat catatcgaac
     3001 agtcaagccg tagggcgagc attcttgccc accgttgcaa gggcgggatt gggtgggcag
     3061 gaatgcccac cctgcatctg aaacccttcc gtgtcattcc cgtgcaggaa ggaatctaga
     3121 tatttgaatt tacggaaatt ttcaatcgtt gccaaagcgg aaaagggttt ttattacagc
     3181 ctaatccttt gggtatcaaa gccgggcggt gttgaaacat tgcaaaaatg ccgtctgaaa
     3241 cccgaaatcg gtttcagacg gcattggttt atgcggcagg ttggggctta gcggtctttg
     3301 taacggcgtt cgcctctgcc tgcaaaagaa tcgccggct
//
LOCUS       DAWHXZ010000193         3337 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000193 DAWHXZ010000000
VERSION     DAWHXZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.193"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..1194)
                     /gene="clpX"
                     /locus_tag="WIE35_001780"
     CDS             complement(<1..1194)
                     /gene="clpX"
                     /locus_tag="WIE35_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ4154661.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIAWQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVG"
     gene            1384..1755
                     /gene="rbfA"
                     /locus_tag="WIE35_001781"
     CDS             1384..1755
                     /gene="rbfA"
                     /locus_tag="WIE35_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ4154662.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGMNLSALIDQVAAEKPVED"
     gene            1788..2711
                     /gene="truB"
                     /locus_tag="WIE35_001782"
     CDS             1788..2711
                     /gene="truB"
                     /locus_tag="WIE35_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ4154663.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIVATARAD
                     ISLAEFQTACKALTGDIRQVPPMFSALKHEGKPLYEYARKGIVIERKARYITVYAIDI
                     AEFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLSCDVLVSHFPQTVLNDYAVHMLRCGQRPRFEEDLPSGTPVRVYT
                     EIGEFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            2928..>3337
                     /locus_tag="WIE35_001783"
     CDS             2928..>3337
                     /locus_tag="WIE35_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154664.1"
                     /translation="MKTVIQTELTQALLSHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDLRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRS"
BASE COUNT          856 a          907 c          787 g          787 t
ORIGIN      
        1 accgaccacc acttttttca agccttgcaa atcaggcagg cggtacatcg tatcgagcag
       61 gcagcgttcg acgatggaac gcaggccgcg cgcgccggtt ttgcgctcca ttgcctgcca
      121 cgcaatggaa cgcaatgcgc cttcttcaaa ttccaactcg acattttcca taccgaacaa
      181 ggcttgatac tgcttgacca aagcgttttt cggctcggtc aaaatattaa tcagcgcgtc
      241 ctcatccagt tcttctaacg ttgcaatcac aggcaaacgt ccgattaatt ccggaatcag
      301 accgaatttg attaaatctt ccggttcgac gatgccgaac agcttggtaa tgtcggcatt
      361 ttcgtccttg ctgtgaacgg acgcaccgaa accgataccg cctttttcag tacgctggcg
      421 gatgactttt tccaagcctg caaacgcgcc gccgcagata aacaggatgt tggtggtatc
      481 gacgttgata aattcctgat tcggatgctt gcggccgcct tggggcggaa cgctggccac
      541 cgtaccttca atcagtttca gcaaggcttg ttgcacgcct tcgccggaca cgtcacgcgt
      601 gatggacgga ttgtcgcttt tgcgtgaaat cttatcgatt tcgtcgatat agacgatgcc
      661 gcgttgggct ttttcgacat cgaaatcaca tttgcccaag agtttggtga tgatttgctc
      721 gacatcttcg ccgacataac ctgcttcggt cagcgtggtt gcatctgcca tcacgaacgg
      781 cacatccagt ttgcgcgcca aagattgcgc cagcagggtt ttacccgatc cggtcgggcc
      841 gataagcagg atgttggatt tcgacaattc gacattagct ccggctttcg ggtggcgcag
      901 gcgtttgtaa tggttgtaca ccgacaccgc caaggctttc ttggcttgtt cctgaccgat
      961 aacgtggtcg ttgaggttgg cgacgatttc ggcgggcgtg gggagcttgc cggattcttc
     1021 cggctcccct ccggcacttt ccgaaggcgt gccgtcattt ccgtcttcat gcaatatttc
     1081 aatacagttt gagacgcatt cgtcacagat aaaggcgttt tcgccctcaa ttaaatgttt
     1141 gacgtgtgat ttggattttc cgcaaaagga acaggtacgg ttttcgttgg acatggcttt
     1201 ctttacaatg tatgcgttac agaaacggca cgcgccgttc gggttgccaa gtataataac
     1261 tatatccgtt cttatcaatg tattacctta aaatcccgcc gattcggcta taatacgccc
     1321 tttcgcaacc gccccggcgg caaaaatgcc gtctgaaacc aaatctgaaa tctgaggata
     1381 ttcatgagaa aaccccaacg cggctatgcc cgccaagacc gtgtcaaaga acaaattatg
     1441 cgcgagcttg ccgaactcgt ccgtaccgga ctgaaagacc cgcgcgccgg cttcattacc
     1501 gtcaacgaag tcgaagttac ccgcgattac agccacgcca ccgtgttcta caccatttta
     1561 aaccaagacg cgcgcgaaat tacggaagaa gtgctggaac acgcgcgcgg acacctccgc
     1621 agcgaattgg ccaaacgcat caagctgttc aaaatccccg agctgcattt caaatacgac
     1681 gaatccttgg aacgcggtat gaacctgtcc gcccttatcg accaagtagc ggcggaaaaa
     1741 ccggttgaag actgacggat atgcccatgc cgtccgaaca tcgaaccatg aatacaggca
     1801 aaccccaaaa acgtgccgtc aacggtgttt tgctcttgga caaacccgaa ggcctttcca
     1861 gcaacaccgc gctgcaaaaa gcgcggcgtt tgttccgtgc cgaaaaagcc ggacataccg
     1921 gcgtgctcga tcctttggca accggacttt tgcccgtctg cttcggcgaa gcgaccaagt
     1981 tcgcccaata cctgctggat gccgacaaag cctacaccgc cacgctgaaa ctcggcgaag
     2041 ccagcagcac gggtgatgcc gaaggcgaaa tcgttgccac cgcccgtgcc gatatttcct
     2101 tagccgaatt tcagacggcc tgcaaagcat taacgggcga catccgccaa gtgccgccaa
     2161 tgttttccgc gctcaagcac gaaggcaaac cgctgtacga atacgcccgc aaaggcatcg
     2221 tcatcgaacg caaagcgcgc tacattaccg tttacgccat cgatattgcc gaatttgacg
     2281 cgcccaaagc cgtcatcgac gtacgttgca gcaaaggcac ctacatccgc accctcagcg
     2341 aagacatcgc caaacacatc ggcacgttcg cccacctgac cgccttgcgc cgcaccgaaa
     2401 ccgccggctt taccatcgcc caaagccaca cgcttgaggc cttggcaaat ttgaacgaaa
     2461 cagaacgcga cagcttgctg ctatcctgcg acgtattggt ttcacacttt ccccaaaccg
     2521 ttttaaacga ttatgccgtc catatgctcc gctgcggaca acgtccgcgt ttcgaggaag
     2581 acctaccttc cggcacgccg gtacgcgttt acactgaaat tggagaattt gtcggtttgg
     2641 cagaatatca aaaggaaata tgccgtctga aagccttgcg cctgatgaac acggcggcat
     2701 ccgccgcctg aacgacggtt aaaaatacgg gctgtgtttg aataatgtgt tgatatttcc
     2761 gcaaaatccc gacacactcg gacaccctcc ccgcttatcg caactttgcg aacgcccccc
     2821 gaaacagaaa agacatcaaa ggattgattt tattagaatt tatttgcaaa gccatttgcc
     2881 gttacacaag aatggcacat taaaatgact gatgaggatt tataacgatg aagacagtca
     2941 ttcaaaccga attaacccaa gccctgctat cacacgaaaa agtatgggcg aacgaagaaa
     3001 aaaccatttt agccaaaaac attctgttgg atttggtgga aaaaaccgac ccgaccatta
     3061 tcggtttgtt attgaataat gatgagttaa aacgccattt ctttgtggaa gtgaatggtg
     3121 ttttggtgtt taaattgcag gatttacgtt ttttcttgga caaacacagc gtcaataatt
     3181 cctacacaaa atacgccaac cgcattggtt tgacggacgg caaccgcttt ttgaaagaca
     3241 attcggatat tgtgttggat tttccgttta aagattgcgt attgaatggc ggacaaagca
     3301 ccgaagaagg cgaagagatt tattttaaac gcagtaa
//
LOCUS       DAWHXZ010000194         3306 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000194 DAWHXZ010000000
VERSION     DAWHXZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.194"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(142..780)
                     /locus_tag="WIE35_001784"
     CDS             complement(142..780)
                     /locus_tag="WIE35_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ4154665.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            1035..1877
                     /locus_tag="WIE35_001785"
     CDS             1035..1877
                     /locus_tag="WIE35_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ4154666.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            1943..2605
                     /gene="exbB"
                     /locus_tag="WIE35_001786"
     CDS             1943..2605
                     /gene="exbB"
                     /locus_tag="WIE35_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ4154667.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            2608..3042
                     /locus_tag="WIE35_001787"
     CDS             2608..3042
                     /locus_tag="WIE35_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ4154668.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
BASE COUNT          868 a          779 c          931 g          728 t
ORIGIN      
        1 cttgctctat tattcatgct aagttaaatt gaggacacgc cctaataatg atgaaaaaaa
       61 taagattaca ctacaggaaa acgcggcagg gcaatatgtc ctgccgcgtt ttctggagcg
      121 tttatgccgt ctgaaagcca gtcagacgaa catctgcaca aacgcaatca ccagccgcac
      181 aatcggtgca ataaacgcac ccaaaacccc ggtcagcatc agcagtagga taatccacgt
      241 cccataaggt tcgattttgc ggaacgcttg cgaatatttc gccgacagga aggtgtcgat
      301 gaaaatgccg ccgtcccaag gcaggatggg gatgatgttg agcgcgaaca gaatcgcatt
      361 gatcagaata ccgtagtttg ccatttgagc caacggcatc tgatacgccc caccgacata
      421 cggagtcagc accaaaacca cgccccacag aacagccatc gctagattcg acagcgggcc
      481 ggacgcggca acgcaacgcc aggcaaggcg cgggttgcgg aagttgcgcg aatcgatagg
      541 aatcggacgc gcccagccga acaggaaggg cgtgaacatc aaagtaagca gcggtacgat
      601 gattgtgccg accaaatcga tatggggcag ggggttcagt gtcagcctgc cgtattgttc
      661 ggcagtgttg tctccccagt agcgcgccgt atagccgcgc gccacctccc tgacggtaat
      721 ggcgagcagc acgggcagga cggcaagcag aaacacgccc aaatcaaaat tttgaaacat
      781 ggttttcctt tgaacagatg tggtgtgcag cccgaaacgg ctgacagaca gaagcggcaa
      841 tatatcatct aacctgattt tattggaata agaaatcaga cggaatcatt ttttctgccg
      901 gcccgttatc tgatgatttg ctgtaataca gcgtttaaac acggtttgcc tgtgaacgga
      961 acagacattc atatgaccaa aaattgtaaa aataggttgc aaataggaat tgttgctatt
     1021 attatgcccg aattatggat aaagaacgaa ttttaacccc cgcagtcgtg ttttccgtcg
     1081 cactgctgca tttggcaatg gtggcattgc tctggcaggc gcacaagctg cccgtgatag
     1141 agtcaggaaa tgttatcgaa tttgtcgatt tgggtgattt tggcggaggg gacggtgcac
     1201 ccgaaggcgc aggtgcgcct gctgcgcctg agccgcaacc tgagcctgag ccgcccaaac
     1261 ctgtcgagcc gcccaagccg gttttgaagc cggtggttac gaaaaaggcg gatgcggata
     1321 ttcagcagcc taaggaagag ccgaaacctg aagaaaagcc gaaacccgaa gaaaaaccga
     1381 aaccagagcc taagccggaa gcgaagcctg tcccgaaacc ggcggaaaaa ccggtcgaga
     1441 agccgtctga aaaacctgcc gaacatccca gcaatgcttc tgccaaagca gacagcgagc
     1501 agggtaatgg ggaagataag ggaaccggta ccaaaggaga cggaacgggg cgcggagaag
     1561 gcagcggtaa aggtagcggc ggtgtcaaag gcgaacacgg ggaaggagcc ggcagcagca
     1621 aaggcaatcc tttacgcgcc aacggcagca ttccgcgccc ggcttatccc acgctttcta
     1681 tggagaatga cgagcagggt acggttgttt tgagcgtttt ggtttctccg ggcggtcatg
     1741 tcgagtccgt taaaatcgtg aaaagcagtg gtttttctcg gttggacaat gcggcacgca
     1801 aggcggcgca aaacgggcat tttcaagcca atgcctggac ggagtttaaa gtccccgtca
     1861 agtttgaatt gaattaggcg gttgccgcag cggtttgcgg cagcttggat gtttgattgg
     1921 ttttttcagt gttggaatca ttatgaattt gaaattagta tttgaatcgg gcgatcccgt
     1981 cctgatcggt gtgtttgtgt tgatgctgtt gatgagtatc gtaacgtggt gtttggttgt
     2041 cttgcgctgc atcaagctgt atcgggcgcg caaagggaat gccgccgtca aacggcatat
     2101 gcgcgatact ttgtcgctga acgacgcggt cgaaaaagtg cgcgccgtcg atgcgccttt
     2161 gtccaaactg gcgcaagagg cattgcagtc ttaccgcaac taccgccgaa acgaagcgtc
     2221 cgaactggcg caggctttgc cgttgaacga gtatttggtc attcaaatcc gcaacagtat
     2281 ggcgcagatt atgcgccggt ttgattacgg gatgaccgcg cttgcctcca tcggcgcgac
     2341 cgcgccgttt atcgggctgt tcggcacggt ttgggggatt taccacgccc tgatcaatat
     2401 cgggcaaagc gggcagatga gtattgcggc ggttgccggc ccgattggcg aggcactggt
     2461 ggcgacggcg gcgggtttgt tcgtggcgat tccggcggtg ttggcataca acttcctcaa
     2521 tcgcggcaca aaaatactga cccaggattt ggatgcgatg gcgcacgatt tgcacgtccg
     2581 cctgcttaat caaaaggata gctgattatg gcatttggtt cgatgaattc cggcgacgat
     2641 tctccgatgt ccgacatcaa cgttacgccg ttggtggacg tgatgctggt gttgctgatt
     2701 gtgtttatga ttactatgcc ggtgctgacg cattccatcc ctttggaact gccgaccgcg
     2761 tccgagcaga caaacaagca ggacaaacag cctaaagacc ccctgcgcct gacgattgat
     2821 gcgaacggcg gctattatgt cggcggggat tctgcaagca aagtggaaat cggggaagtg
     2881 gaaagccgtc tgaaagccgc caaggagcag aatgaaaacg tgattgtggc gattgcggca
     2941 gacaaggcgg tggaatacga ttatgtaaac aaagctttag aagccgcccg tcaggcagga
     3001 atcaccaaaa tcggttttgt aaccgaaacc aaggcgcaat aaagttcgac gcttgaacga
     3061 atgccgtctg aaagtctttc agacggcatt tcttatgttt aatcagctat atatagttga
     3121 ttaaaacaaa aatgagacga cagagcaccg tctccatttc tgcgcaggcg ggaatacaaa
     3181 catctgattt ttcttttatt ttcaaacatt actgaaatgg gtaagtctgg agtcctgcct
     3241 gcgcgagaat aatgaaaatg tgcatttcta atttttatag tggattaaca aaaatcagga
     3301 caaggc
//
LOCUS       DAWHXZ010000195         3254 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000195 DAWHXZ010000000
VERSION     DAWHXZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.195"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            197..1138
                     /gene="miaA"
                     /locus_tag="WIE35_001788"
     CDS             197..1138
                     /gene="miaA"
                     /locus_tag="WIE35_001788"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ4154669.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIIPPTESYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSTHLGSLTSHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPSLTADSPAIRCVGYRQAWKYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            1142..1861
                     /locus_tag="WIE35_001789"
     CDS             1142..1861
                     /locus_tag="WIE35_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEZ4154670.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLDGTPPQEMSQAHKARLLAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIAAAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            1866..2354
                     /locus_tag="WIE35_001790"
     CDS             1866..2354
                     /locus_tag="WIE35_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ4154671.1"
                     /translation="MTALLVILALALIAAGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYMAGIWGTKYTGAGKLAVRGALVGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLIVGTTFKIGCAVSILFILLVKYIAY
                     LF"
     gene            2420..3172
                     /gene="rlmB"
                     /locus_tag="WIE35_001791"
     CDS             2420..3172
                     /gene="rlmB"
                     /locus_tag="WIE35_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ4154672.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYVQEGKNDVRTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
BASE COUNT          768 a         1038 c          820 g          628 t
ORIGIN      
        1 atcggacgct gccgccgatg cctgaggaca aggctgcatc gggcgcgggc atattgaaaa
       61 cccggcgcgt cggtttgaaa ttttataaag gcatacggta aacacgcccc cttgccgtcg
      121 gcgtgcttcc gcaaaccaaa atataacgtc ggcacgcttt ccgctataat ccgtattttt
      181 ccaaacctga aacgttatgc cgaccccgaa agcctttgcc ctgctcgggc cgaccgccgg
      241 aggcaaaacc gcgctcgccc tcaaaattgc cgaaaccctg cctgtcgaaa tcatcagcct
      301 cgattccgcg ctggtttacc gcgatatgga catcggcacg gcgaaaccgt ccgcctccga
      361 acgcgccttt gtcccgcacc accttatcga catcatcccg cctaccgaat catacagcgc
      421 cgcccgcttt gtcgaagact gcacgcgctt ggtcggggaa atttcctcac gcggcaggtt
      481 tgcccttatt gtcggcggca cgatgatgta tttccgcgcc ctgacccaag gtttgaacga
      541 tttgcccgaa gccgacgcct gcctgcgtgc cgacttggac gaacaaaaac agatgtacgg
      601 cttggatttc ctctaccgca ccctgcaaaa agtcgaccct gaaaccgcat gccgtctgaa
      661 accgaacgac agccagcgta tcggacgcgc tttggaagtt tattatttaa ccggcaaacc
      721 gatgagcaca catctcggca gcctgacctc ccatacgctc ccttttgatt tgcataccgc
      781 cgccctgatt cccgaaaacc gcgcccgcct gcatgaaaac atcgccctgc gtttccacct
      841 gatgcttgaa cagggcttta tcggcgaagt ggaaaacctg cgccgccgct atcccagcct
      901 gaccgccgac tcccccgcca tccgctgcgt cggctaccgt caggcatgga aatatctgga
      961 tggaaaaacc gatttcccgg catttgtcga gaaaggcatt gccgccaccc gccaacttgc
     1021 caaacgccaa ctgacttggt tgcgcaaaac acctttagac tgcgttgccg acccgttttc
     1081 agacggcact tcatgcacac gcctgattga ggcggcaaaa cggtttttcg gcgcataaac
     1141 catgctgacc acaccgccgc tcgcccccaa aaccgtcgcc gccctgcaca ggctcggtat
     1201 ccgtacgctc gaagaactgc ggcaaaacgg ttcggtcaaa gcctttctgc tgctcaaagc
     1261 atcgggtttg accctcacga aaagcacgct gtggcaactc gagtcgctgc tggacggcac
     1321 gccgccgcaa gaaatgtccc aagcgcataa agctcgcctg cttgccgaat tgaaaaacca
     1381 tccgcccgtc gccgcctttc cgccgcagga agaaatggaa cacttcatgc gcgaagcact
     1441 gcgccaagca gaacaatccg ccgccgacgg cgaaataccc gtcggggcag tcatcgtttc
     1501 agacggcaaa atcatcgcgg cggcacacaa cacctgcatt gccgactgca atgtcagccg
     1561 ccacgccgaa atcaacgccc tggcacaggc aggcagcgaa atgcaaaact accgccttga
     1621 cggatgcgac atctacatca ccctcgaacc ctgcgccatg tgcgcgtccg cactgataca
     1681 ggcacgaatc aggcgcgtga tctacggtgc ggcagaaccc aaaaccggcg cggcaggaag
     1741 catcgtcaac ctgtttgccg acaaacgcct caatacgcac accgccatac ggggcggaat
     1801 cctgcaagaa gaatgccgcg ccgtattaag ccgttttttc caaaacaaaa gaaaaggttg
     1861 aacctatgac cgccttgctc gtcatcctcg ccctcgccct gatagccgcc ggtacggcgg
     1921 gcatcgttta ccccgccctg cccggattgg cattgatgtt tgccggaacc tggctgctcg
     1981 cctactccgg cggctaccaa atctacggcg cgggcgtttt gtggacggtc ggactcatca
     2041 gccttgccgg catactggcg gactatatgg caggcatatg ggggacaaaa tataccggag
     2101 cgggcaagct cgccgttcgc ggcgcattgg tcggcagcat catcggcata tttttctccc
     2161 ttcccggact aatactcggt ccctttatcg gcgcggcggc aggcgaactg atcgatcggc
     2221 gcaatatgct tcaggcaggt aaagcgggct tgggtacgct gttggggctt atcgtcggta
     2281 cgacattcaa aatcggctgc gccgtatcca tcttgtttat cctgttggtg aaatacatcg
     2341 cctacctgtt ttaacccctt cagacggcat accggcaaat tgctataatg ccgtctgaac
     2401 ccttttcaca aagcaccata tggcaaacca aagacctatc tacggcttcc atgccgtcaa
     2461 cgcccgattg tggcaaaacc ccaaatccat cgtcgaactc tatgtccaag aaggcaaaaa
     2521 cgatgtacgc acgcgcgaag tgttggaaaa ggcggcaaac gaaaacatcc gcgtatattt
     2581 tgccgatgcc gaccgcctca acgccatcag caaaggtgcg cgccatcagg gcgtggtcgg
     2641 atttatcgat gcctccaaaa accacgtcca cctcgaagac gtattggaaa acttaagcga
     2701 accgccgctg ctgctgatac tcgacggcat caccgatccg cacaacctcg gcgcgtgcct
     2761 gcgtaccgcc gacgcaatgg gcgtacatgc cgtcatcgca ccgaaagaca aaagcgcggg
     2821 gctgaacgcc accgtcagca aagtcgccag cggcgcggcg gaaaccgtcc cctacatcac
     2881 cgtaaccaac ctcgcccgca ccctgcgcga gctgaaagaa tacggcattt ggatcatcgg
     2941 caccgatatg ggcggcaacg ccgaccttta ccattgcgat ctgcccgaca gcgcggcatg
     3001 ggtgatgggc aacgaaggcg acggtatgcg ccgcctgaca cgcgaacatt gcgacatgct
     3061 ggtgtccata cccatgttcg gcacggtcga aagcatgaac gtctccgtgt ctgccggaat
     3121 ggtattaagc gaaacccgcc ggcagcgcgt attaaaaaac gaaaaagcgt aagcctcaaa
     3181 aacaatgccg tctgaaacag ggattctgtt tcagacggca ttcgttttca aaatcagtgt
     3241 tccaagtggt cggt
//
LOCUS       DAWHXZ010000196         3219 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000196 DAWHXZ010000000
VERSION     DAWHXZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.196"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            356..2236
                     /locus_tag="WIE35_001792"
     CDS             356..2236
                     /locus_tag="WIE35_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ4154673.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFGPEELDALDGALQKGWQ
                     KGLHSVLFADYEFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDDLPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPNTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLKPCAGGLGFEGIFNV
                     VIRTLSLKPVSASDGIVSGIGGPDSNAQARTAGQGGATPHPFDSNPPYRGVYGVGSGI
                     VIDSDPAAEYRECGWKARFLNELRPDFGIFETLRAENGRCTLLDRHLCRLKTSAQALN
                     LPLPDGCENQIKQYIADLPDGAFRVKALLASDGISLSRAVLNRLTDKQRVIISPTILP
                     AQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKYQGQWLT
                     PSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFAAA
                     LA"
     gene            complement(2295..>3219)
                     /locus_tag="WIE35_001793"
     CDS             complement(2295..>3219)
                     /locus_tag="WIE35_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ4154674.1"
                     /translation="AVNRNANNAAPQYCRQNGGRQICSVSRAERAAGLNYEIEAEKLT
                     ALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRAYLGWQYKNARQTAGILPFYQVQLS
                     GSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTYRPNPGWQFSVALEHYRQRYREQDR
                     AEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRFVPKRETVGGAVNNAAYRRNGVYAG
                     WAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEAQRNREWNVSLALSHDKLSYKGIVP
                     ALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
BASE COUNT          744 a         1038 c          752 g          685 t
ORIGIN      
        1 agccgcagac agtacaaata gtacggaacc gattcacttg gtgcttcagc accttagaga
       61 atcgttctct ttgagctaaa gcgaggcaac gctgtactgg tttttgttaa tccactatac
      121 ttggagctgg tcttgctttt cgcctaattc tacgttttca aacggttgca gctggtggtc
      181 tgtcataaag gtctccttat tgtatttcag gttggaaatc ggaatttgtt ttcacaattt
      241 tacaccttcg ccccccactt tctctacata aaattacatt ttgccgatat ttgccgaatc
      301 gtctgaaaat atgtgtaaaa agagcgtata atcaaaacat ttgccccgaa ttgccatgcc
      361 ctatttcgcc ctgtttgacg atgccgtaag cggccgcgca aaacgctatc aaaatcatgt
      421 agaaagccgt tttttcggtc ccgaagaact cgatgctttg gacggcgcgc tgcaaaaggg
      481 ctggcaaaaa gggttgcatt cggtgttgtt tgcagactac gaattcggtt tgccgctgac
      541 gggggttgag tccgagcgcg gcggcaacct tgccctgcac tggtttgccg actgcgccga
      601 caccgatgcc gcaagctggc ttgcccgaca ctcagacgac cttcccgccg gcatttccac
      661 gccgcaatcc tccgtatccg aaaccgatta cctcgaccgc atccgccaaa tccacgaagc
      721 catccggcgc ggcgacacct atcaaatcaa ctacaccacc cgcctgcacc tgcaagccta
      781 cggcaatccc gtcagcctct accgccgcct gcgccagccc gtcccctatg ccgtcttgtc
      841 ccacctgccc gatgcggagg ggcaatccgc gtggacgctg tgtttctcgc ccgaactctt
      901 cctcaacatc gcttcagacg gcaccatcag caccgaaccg atgaaaggca ccgcgccgat
      961 tttgggcgac ggacaagacg aacgccgcgc cgccgaattg caagccgacc cgaaaaaccg
     1021 cgccgaaaac gtgatgattg tcgatttgct gcgcaacgac ctcggcaaaa tcgcccaaac
     1081 cggcaaagta tgcgtgcccg agccgtttaa agtgtcgcgt ttcggcagcg tttggcagat
     1141 gaccagcacc atccaagccc aagccctgcc aaacacctcg ttcgccgaca tcctccgcgc
     1201 cgccttcccc tgcggcagca tcaccggcgc gcccaaaaaa atgagtatgc agattatcga
     1261 atcgctcgaa accgaagcgc gtgggcttta tacgggcagc atcggctacc tgaaaccttg
     1321 cgcgggcggg ttggggtttg aaggcatatt caacgtcgtc atccgcacct tgtcgctcaa
     1381 acccgtttcg gcttcagacg gcattgtttc aggcatcggc ggcccggatt caaacgcgca
     1441 agcccgaacg gcagggcaag gcggggcaac gccgcatccg tttgactcca atccgccgta
     1501 tcggggcgtg tacggtgtcg gttccggcat cgtcatcgac agcgaccccg ccgccgaata
     1561 tcgcgaatgc ggctggaaag cccgtttcct caacgaattg cgccccgact tcggcatttt
     1621 tgaaaccctg cgcgcggaaa acggacgctg caccctgctc gaccgccacc tatgccgtct
     1681 gaaaacctcc gcccaagccc tcaacctgcc cctgcccgac ggctgcgaaa atcaaatcaa
     1741 acaatacatt gccgacttgc ccgatggcgc gttccgcgtc aaagccctgc tcgcttcaga
     1801 cggcatcagc ctgtcccgcg ccgttttaaa ccgtctgacc gacaaacaac gcgtcatcat
     1861 ttcgccgacc atcctgcccg cacaaaacta cctgcgccgc ttcaaaacca cctgccgcac
     1921 cgtcttcgac caagcgtggc aaaccgccga aacacaaggc gcgttcgaca gcctgttttt
     1981 caattcagac ggcatcctgc tcgaaggcgg cagaagcaac gtgttcgtca aataccaagg
     2041 acaatggctt acgccctctt tagatttaga cattttaaac ggcataatgc gccaagccgt
     2101 attggacgaa ccgcaaaaat atttgcaaac aaatcaagta atcgaaacac acatcacaca
     2161 aaaaacactg caagaagccg aagaaatccg cctctccaac gccctgcgcg gcgtatttgc
     2221 cgccgccctt gcctgaacgc gcaaaaatgc cgtccgaacc tgtttccaaa gttcggacgg
     2281 cattatccca ccattcaaaa ccgccaatcc gccgacacaa acacctcgct gttgcggcgt
     2341 ttcgcatacg gcacattact ttccgtcctg ccgaaacgat aattcaacgc cggcacgata
     2401 cctttgtacg acaacttgtc gtggctcaaa gccagcgaga cattccattc gcggttgcgt
     2461 tgcgcctctg tcgagaaagc cgcaatgccc ttatagttgc ggcgggcata agacgcagaa
     2521 acccgactgt tcaaaccgcc caactgccgc cactcctgcg cccaaccggc ataaacaccg
     2581 ttgcgccggt aggcggcatt attgaccgcg ccgcccaccg tttcgcgttt cggcacaaac
     2641 cgcacaaact gccagccgcc gaacacagtt gccgattcgc ccaaacgttt tgccgacgaa
     2701 acataaaacc cgtcctgcct gccgttattg tattccgccc tatcctgttc gcggtagcgt
     2761 tggcggtaat gttccagcgc gaccgaaaat tgccatcccg ggtttgggcg gtaagtatgg
     2821 gacaactgca cgccgactcc gtgcgccagc atatacggcg gcaggcggcg gttgtttacc
     2881 cgttttgttt tcgcatcaaa gccgtcgctg cccgacaact gcacctgata aaacggcaaa
     2941 atccccgccg tctgccgtgc atttttatac tgccaaccca aatacgctct gccgaacccg
     3001 tcgtcataag ctgatttttt actgaaataa tagctcgtgc cgccgatatt ggaacggaac
     3061 aacaaataat gattatctgc caacgccgtc agtttttccg cctcgatttc ataattcaag
     3121 cctgccgccc gctccgcccg gctgacactg catatctgcc ggcctccgtt ttgccggcaa
     3181 tactgcggcg cggcattatt ggcatttcta ttgaccgcc
//
LOCUS       DAWHXZ010000197         3218 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000197 DAWHXZ010000000
VERSION     DAWHXZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.197"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(281..2446)
                     /locus_tag="WIE35_001794"
     CDS             complement(281..2446)
                     /locus_tag="WIE35_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ4154675.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDDDHRIVLSHMKDQHRGIRTVREEFTVGDKSSRINIDR
                     QAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPNH
                     TRITTRGANFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSKFEISDKKKGADGKEVD
                     VDDAQKEKNRANEKIVHAYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTH
                     DGKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGT
                     KAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIKNH
                     GYELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFK
                     NPNLEIGWRGRYVQKAVGSILVAGQKDRSGKLENVVRQGFGVNDVFANWKPLGKDTLN
                     VNLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          708 a          746 c          904 g          860 t
ORIGIN      
        1 ttctaaggtc tagattcccg cctgcgcggg aatgacggct gcagatgccc gacggtcttt
       61 atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac aaatagtacg
      121 gaaccgattc acttggtgct tcagcacctt agagaatcgt tctctttgag ctaaggcgag
      181 gcaacgccgt actggttttt gttaatccac tatatatcaa attatcagaa cagatgccgt
      241 ctgaaaggct ttcagacggc attttttcgg gatgtgcgtt ttagaacttg tagttcacgc
      301 ccaagcgtac atcgcggccc acgcccggca gggtattggt ccagcgttgg ctgtgcggat
      361 agtagaacgt gttgaacacg ttgttaaccg aaagattaac attgagcgtg tctttgccca
      421 gcggtttcca gttggcgaag acatcgttca caccgaaacc ttggcgtaca acgttttcca
      481 atttgccgct gcggtctttt tgacctgcca ccaatatcga acccacggct ttttgaacat
      541 agcgtccgcg ccagccgatt tccagattcg ggtttttgaa gcggtaggca agggaggctg
      601 tccaagtgcg gccgacttgt gcgccaaatt caggattcgc gctcaacagc ttgtctttgt
      661 gcgtatcgta aaagcgcggt ttgctgtggc ttacgccgac tttggcagtc aggccgccgg
      721 tgcggtagga cgcgcccaat tcgtaaccgt ggtttttgat gtaaccggca ttgacggctt
      781 cacggacagc ggcagagacg tggcggtttt gcggattggc aagcgcgtct ttgatggttt
      841 gccagaagta gctgccgttg gcggcaaacg tgccgtcgtt gtagttgaag ccgatttcgg
      901 tattgcgcgc gcgttcggct ttcgtgccgt cggcaatcga gatgatgccg cgtttaccgt
      961 gggtttgcag cgcgtcatac aggcgcgggc tgcggctggc gtagttgtgg ctcgcgctga
     1021 agctccagtg ttcgtgcggc tgccaaatca caccgaaact cgggttaagg ttgctgcttg
     1081 aaacggtttt gccgtcatgg gttttcacct tgaagcggtc gtaacgcagc ccgccggtca
     1141 gggtaaagcc gtcaatctcg tgaatggctt cgatatacgc gccggtatcg gttttggtcg
     1201 ggttagacag tttgtaggcg tgaacaattt tttcattggc acgatttttt tctttttgtg
     1261 catcatctac gtcgacttct tttccgtcgg ctcctttttt cttatcggag atctcaaatt
     1321 tcgagttcaa aaacgcttgc ggtttgattt cctgatggcg gtagttgata ccgtatttca
     1381 gcagggtttg ttcggcaagg cggctgtcga agttgaagtt cgcaccacga gtggtgattc
     1441 gggtatggtt ggggcctttt acattgcctg cgtagccggt gccgctgtca tcggcggaat
     1501 agcgttcttt ttccaacaca taggcgttgg catccagttt ttcgacaaag cccaggtttt
     1561 tacccgtgta cgccaagttg gtgttggatt gggtagtttc gcggtaagca ggggcttggc
     1621 ggtcaatatt tatccgtgaa cttttgtcgc cgacggtaaa ttcttcacgc acagtgcgga
     1681 tgccccggtg ttggtctttc atgtggctca acacgatgcg gtggtcgtcg tcgccgaaag
     1741 ttgttccgat tttggcgagg tagctgcgtt tgtccagcgc gctgtacggt acggttttgc
     1801 cgccgttgac attgcggaaa cctttgccgg cttcgtaatc tttttcatcg ttgcggttgt
     1861 aagagaacaa accgtcgaag ttgccctctt ttccgaatac gctggcgccg tagcttacgc
     1921 cttcgttgct ggcaaagccg ctgttgaggc gcacgcccca gtttttatcc aagcctttga
     1981 gcaggtcttg ggcatcgacg gttttggcga taatcgcgcc gttggtcgcg ccgataccgg
     2041 cagaggcgga acccgcgcct ttttgtacgg aaacgacttt aaccaaagcg ggatcgacaa
     2101 taaatctgcc ttggtggtaa aggatttggc tgtcggaata ggcgttgtcc accttgatgt
     2161 cgacagagtt ctgacccata ccgcgcagcg tcaggaattg ggacgtgccg ttgccgccgc
     2221 cgaaatcgat ggacggctct tcttttaaga gttcgcgcat atcggttgcg gtgctttcgt
     2281 ctttttgttg aagcgtaacg atgttggtac ggattttgct gccttggcgg tcgcctttta
     2341 cggtaacggt atccaatgcg acattggcat tattttctgc cgcatgggca aaacctgccg
     2401 ccagggtaag cgagagcagg ctgaggcgga aaaacggggc gttcatttgt tcgtcctttt
     2461 gagtgtatga agggaagtaa atccaaaccg ttaaggtttg gcaggataag aaaaataatt
     2521 aataattatt tttatttata ttaacagggg ggggggattt ttgcaaagct gattatcgtt
     2581 tttatttgcg aagtgttgtt ttttgttgac aagttttgtc ggaaatgtaa aaaacggcgg
     2641 aaatatagtg gattaacaaa tgcgggaatg acgaaaagca acctttccgc gtccttcccg
     2701 cgaaagcggg aatctagaac ccctaacggg caggaatcta tcggaaaaaa ccgaaaccga
     2761 acagactgga ttcccgcctg cgcgggaatg acgaagcctg cgcgggaatg acgaaaagca
     2821 acctttccgc gtccttcccg cgaaagcggg aatctaggaa ctcaaagctg caagaattta
     2881 tcagaaacaa ctggaactca aaaaaactag attcccgcct gcgcgggaat gacgaagggt
     2941 taaagaaatg atgagaaacg gcggtaattt atcgggaata accgaaaacg aacggaaatc
     3001 aggacaaggc ggcgaagccg cagacagtac ggataggacg gaaccgattc acttggtgct
     3061 tgagcacctt agagaatcgt tctctttgag ctaaggcgag gcaacgacgt actggttttt
     3121 gttcatccac tataacagca accctgtcgc cgtcattccc gcgaaagcgg gaatccagtc
     3181 cgttcagttt cggtcatttc cgataaattc ctgctgct
//
LOCUS       DAWHXZ010000198         3198 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000198 DAWHXZ010000000
VERSION     DAWHXZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.198"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            358..981
                     /locus_tag="WIE35_001795"
     CDS             358..981
                     /locus_tag="WIE35_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ4154676.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIMLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1092..2474
                     /locus_tag="WIE35_001796"
     CDS             1092..2474
                     /locus_tag="WIE35_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ4154677.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQDGLQCALSQLEGVEDIGFSFDRQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2478..3002
                     /locus_tag="WIE35_001797"
     CDS             2478..3002
                     /locus_tag="WIE35_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ4154678.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          656 a          862 c          952 g          728 t
ORIGIN      
        1 ctcaagatat agtggattaa atttaaatca gtacggcaag gcgaggcaac gctgtactgg
       61 tttaaattta atctactata tttccgcctg ctgccgcccg aaaagcgtga tgcgctgatg
      121 gtgctgctgc acaatccgga cgacggatag ccgttgaaaa cgggaaatgc ccgggcggtg
      181 tcgcctgtgc ctgtttggac aagcgttctt gagggcggta gaattgaggt ttgcccgaaa
      241 agggcagggc gatatgccgc gcttggattt tgcgcgcggt ttttcgcgtt ggaagcaatc
      301 attgtctgtt tgaatattta atgccgtctg aaacgtccgg acgcgccccc gttccctatg
      361 aaaaaaccga ccgataccct acccgttaat ctgcaacgcc gccgcctgtt gtgtgccgcc
      421 ggtgcgttgt tgctcagtcc tctggcgcac gccggcgcgc aacgtgagga aacgcttgcc
      481 gacgatgtgg cttccgtgat gaggagttct gtcggcagcg tcaatccgcc gaggctggtg
      541 tttgacaatc cgaaagaggg cgagcgttgg ttgtctgcca tgtcggcacg tttggcaagg
      601 ttcgtccccg aggaggagga gcggcgcagg ctgctggtca atatccagta cgaaagcagc
      661 cgggccggtt tggatacgca gattatgttg gggctgattg aggtggaaag cgcgttccgc
      721 cagtatgcaa tcagcggtgt cggcgcgcgc ggcctgatgc aggttatgcc gttttggaaa
      781 aactacatcg gcaaaccggc gcacaacctg ttcgacatcc gcaccaacct gcgttacggc
      841 tgtaccatcc tgcgccatta ccggaatctt gaaaaaggca acatcgtccg cgcgcttgcc
      901 cgctttaacg gcagcttggg cagcaataaa tatccgaacg ccgttttggg cgcgtggcgc
      961 aaccgctggc agtggcgttg attttgaacc cgcgccgcaa ccgaaatacg gcgaatcctg
     1021 tataatccga aaatctgttc actggaagtt cagacggcat tgcaactgtt gatgccgtct
     1081 gaaaaaatat gatggcaaga gataaccgca tccaaatgtt tccgcacgaa tggcgcgcca
     1141 gtacgacgct ttccggcgtg tacgcgctgc gtatgctggg tatgttcctc gtgctgcccg
     1201 tattggcggt gtatgccgcc tcgctgcccg gcgcggaagg caacaaaacg ctggtcgggc
     1261 tggcaatggg catttacggg ctgacacagg ctctgctgca actgcctttg ggcatcgctt
     1321 ccgacaagtt cgggcgcaag aaaaccattt atgtcgggct gattgtgttt gcagcgggca
     1381 gcttccttgc cgccgccgcc gatacgctgc ccatgctggt cgccgcacgc gccatacagg
     1441 gtgcgggagc ggtcagtgcg gcggttaccg cgctgctggc ggatttgacg cgcgacggcg
     1501 tacgtacccg cgcgatggcg atgatcggtt tgagtatcgg tctgacgttt tcggtcagcc
     1561 tcgtcgttgc ccctgtgatt gccgacgcgg tgggcgtacg cggcttgttt ctgctgaccg
     1621 gtattttgac cgttgccagt atcggcgtgg tggcgtggat gactcccgac ccggaggttt
     1681 ccaagctgca cgaagatacg caggcgcagc cttcgcgcat aggcgaagtt ttgaaaaacc
     1741 gtaggctgct gacgctcgat ttcggcattt tcgccctgca cgccgcgcaa atggcattgt
     1801 ttaccgcgct gcctttcgcg atgacccagc tcggtttgga aaaaatccag cactggaaag
     1861 tctatctgcc ctcgaccatt acgggattgg tggtaatggt tccgctgatt atcgtcggcg
     1921 agacgcgcaa caagcttaag caggtttttg ttttgggtat cgtctgtatt gcggcggcgc
     1981 agctcggttt gctgtccggt atgcgctcgg taggcttgat taccgcttat ttggttgttt
     2041 actttatcgg ttttaatgtg ttggaagcga gcctgccgtc tatggtttcc aaaatcgcgc
     2101 cgtccgacct gaagggtacg gcgatgggcg tgtacaacac gatgcagtcg ctcggactgt
     2161 ttgccggcgg cgcggcaggc ggtttgctgt ttcaaaaata cggcttttcc ggcgtgtttg
     2221 ccttttgcag tatattgatg ctgctgtggc tggtaattgc cgttttatcg cccgcgccca
     2281 agcccgtcaa aaacctcagt taccctgtcg gcggcgtgtg gcagggcaat caggacggct
     2341 tacaatgcgc cttgtcgcaa ctcgaaggcg tggaagacat cggtttcagt ttcgacaggc
     2401 agaccgtcta tctcaaagtg ttgcagaagg gtttcgatca ggctgccgct gaaaaaatca
     2461 tcacaggagt ttaaaaaatg tcattgaaca aagtcatcct tatcggccgt ctcggacgag
     2521 atcctgaagt acgctatatg cccaacggcg aagccgtctg caatttcagc gtcgccacca
     2581 gcgaaacttg gaacgaccgc aacggtcagc gcgtagagcg caccgagtgg cacaacatca
     2641 cgatgtaccg caaactggcg gaaattgccg ggcaatacct caaaaaaggc gggctggttt
     2701 atctggaagg cagaatccaa agccgcaaat accaaggcaa agacggcatc gaacgcactg
     2761 cttacgatat tgtcgccaac gaaatgaaaa tgttgggcgg gcgcaatgaa aacagcggcg
     2821 gtgcgcctta cgaggaaggt tacggtcaga gtcaggaggc ttaccaacgc cccgcgcagc
     2881 aaagccggca gcccgcctcc gacgcgccgt cccatcccca agaagcacca gccgcgccgc
     2941 gccgccaacc cgtgcctgcc gccgccccgg tcgaggacat tgacgacgac atcccgttct
     3001 gaattttacg gcagcacatc ctcgcggagg gaaatcataa aggacggaga aaccttaaac
     3061 cttacggggc agggtttctc ttttttgcat ttgtgccggc ttgttgctgc cccctgcgtt
     3121 gggcggaacg ttgcgattgc gccgttctgg cgttttgagg cagtgatgcc tgccgcaggc
     3181 ccacgctgtt ttcagacg
//
LOCUS       DAWHXZ010000199         3197 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000199 DAWHXZ010000000
VERSION     DAWHXZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.199"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..699
                     /locus_tag="WIE35_001798"
     CDS             <1..699
                     /locus_tag="WIE35_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEZ4154679.1"
                     /translation="ATVGSAPYRDLSPLGAEWAEKADGNVRIVGCAVCGEFKKAQVQE
                     QLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNACVVVSCGTAVTV
                     DALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTTGNAVASGMMDAV
                     CGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENTVRVADNLVIHGL
                     LNLIAAEGGESEHT"
     gene            812..1636
                     /locus_tag="WIE35_001799"
     CDS             812..1636
                     /locus_tag="WIE35_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ4154680.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(2399..3187)
                     /locus_tag="WIE35_001800"
     CDS             complement(2399..3187)
                     /locus_tag="WIE35_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ4154681.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
BASE COUNT          721 a          876 c          949 g          651 t
ORIGIN      
        1 gcaaccgtcg gtagcgcgcc gtaccgcgat ttgtcgcctt tgggcgcgga gtgggcggaa
       61 aaggcggatg gaaatgtccg catcgtcggt tgcgccgtgt gcggagaatt caaaaaggca
      121 caagtgcagg aacagctcgc ccgaaaaatc gagtggctgc cgtcttccgc acaggctttg
      181 ggcatacgca accactaccg ccaccccgaa gaacacggtt ccgaccgctg gttcaacgcc
      241 ttgggcagcc gccgcttcag ccgcaacgcc tgcgtcgtcg tcagttgcgg cacggcggta
      301 acggttgacg cgctcaccga tgacggacat tatctcgggg gaaccatcat gcccggtttc
      361 cacctgatga aagaatcgct cgccgtccga accgccaacc tcaaccggca cgccggtaag
      421 cgttatcctt tcccgaccac aacgggcaat gccgtcgcca gcggcatgat ggatgcggtt
      481 tgcggctcgg ttatgatgat gcacgggcgt ttgaaagaaa aaaccggggc gggcaagcct
      541 gtcgatgtca tcattaccgg cggcggcgcg gcaaaagttg ccgaagccct gccgcctgca
      601 tttttggcgg aaaataccgt gcgcgtggcg gacaacctcg tcattcacgg gctgctgaac
      661 ctgattgccg ccgaaggcgg ggaatcggaa catacttaaa aaatacaccc tttttcaaca
      721 cggggcggca tccgtccgaa tccgtccgga ttctgccgcc cgacagactg ccgcccaggc
      781 gggcggggtt tcaaacattg aggaaattct aatgaaatgg ctcttcaccc ttttggcggt
      841 actcaatatt gccgtattcg gcggcacggt gggctataaa ttggccgtta aagcgacggg
      901 cggcgtgccg gaaaaccggg ccgtcgaaaa cacgcccccc gcaacgcccg ccgccgacaa
      961 tgcggcggca tccgtggagg atacggcggc actgctcaaa ccgggcgaca tcctgaccga
     1021 agagcaggcc gagcagttgc gcttgaaaaa ggaagcggaa cagaaaaaac tgagggagaa
     1081 aaaacagcgt gaagaaaaag cccgccgcga aaaactcgcc gccgaaaagg cgcaggcgga
     1141 acgcgaaaac ggcgcggcgg atgccttatg cgccgcgcag gcaagcctca cgatggacga
     1201 agacgactac caccgcatca aagggctttt gggcaaatgg tcgcacgttg ccagcaggag
     1261 cgtcgaaaaa cgcaccgcca aagccaaacc tgccgacaaa acctaccgcg tcgtcctgcc
     1321 cgtttccgcc gatgccgaaa atcaggcggc ggagctgtct gccaaaggtt tcaaccccat
     1381 accgtttgac ggcgcattga gtttgggtgt cggcaacagc cgggaaaacg cccaagccct
     1441 gcaaaaccgg cttgccggcg ccggattcgg cggggcgcat attgtcgaac actttgccga
     1501 agccgacagg caggacgatt ctttgagcgt gtcgcgtatg acggttttgt ttaccggcgt
     1561 gaatgccgcc gatgcggacg aaattcgtaa aatcacgtcc ctatacggca aactgaacct
     1621 caagtcttgc aaataggcgc gaaagccgga cgataaacgc caaacccacc gcgaaggtgg
     1681 gtttggcgtt tcggttttga gtatctgata tataaaaagg gaatgtatgt ctcgtatatg
     1741 ccgactccaa gtgtgaaagt gaattgtgag ggaatacagt gtttaatggt tcggaatgga
     1801 cgacttccga gtggttttga ttatacgcta ataataataa ttatcaataa aaatttaaaa
     1861 ataaatgcgg attttttgta atacgatgaa aaataaacaa attaacctgt ccgccgatgt
     1921 ttgtgattcg ggtcggcaaa ctttcattgt gtcggacaaa tcggggtcgg cagtcatagc
     1981 tgagggcttc gtgcctgacg gaaataagcc tgccgtgttt gaacatccgt ccgatttggc
     2041 gcggacaggg ttgtcattcg ggggtgtttg agcgtggatt cggggatgcg aaagcggtaa
     2101 aaccgttgcg gatgaaggct tacggacggc tttaggtatt tttaagtgta ttcaaacaac
     2161 aacaaaacgc ttcttctatt gccgtattta gggcttgcgc ggcagcctgc acaccgtgga
     2221 ggccgagtgt ttcgttggtt gcggcatcgg gacgggcggc aaggtttttc ggccggtttt
     2281 tccatttttt ccgacaaagc cgtgatgccg gggaatggcg gtttgtccgt ttcaggccgc
     2341 cgccgttttg gaccgcgatg ccgtctgaag ccttcagacg gcatttttgc cgcctttttc
     2401 aatattccgc cgaatgccaa aacggttgtc cgactgtcgg cgtgctggct tgcgctttgt
     2461 cgcgtgcttc gaccggtccg gcttcaaatg ccagccgtcc ggattcgacg gcgagtgcga
     2521 aggcgcgtgc catattgacc ggatcgccgc tgcgggaaac ggcagtattc aaaagcacgc
     2581 cgtcaaagcc ccattccatc acttgtgccg cctgtgaggg caaacccaag cccgcgtcga
     2641 taatcagcgg cgtgtcgggc aggcgttcgc gcaggacgtt caacgcgtag gcgtgaaccg
     2701 cgcccaaacc cgtgccgatc ggggccgccc acggcatcaa cgcctgacag cccgcgtcga
     2761 gcaggcggcg gcaggcaatc aggtcttcgg tgcaataagg cagcactttg aagccgtctt
     2821 taatcaggat ttccgccgct tcgacaagct ggaacacatc cggctgcaag gtgtcgtcat
     2881 ctccgatgag ttccaatttt atccaatcgg tttcaaacac ttcgcgcgcc atttgcgccg
     2941 tcgttaccgc ttcctgcacg ctttggcagc ctgccgtgtt cggcaggacg ggaacgccgg
     3001 tttcttgaag cagcgaccaa aacccctgac cgtgcgcctc gccgccgctt cccgcgcggc
     3061 gcaatgagac ggtaatcatc gcgggttggg cgatacggac ggattgtttg aggatttcag
     3121 gggtcgggta ggcagccgtg ccgagcagca gccgtgaagg gaaagtttct ccgtataggg
     3181 tgagcataat gggttcc
//
LOCUS       DAWHXZ010000200         3154 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000200 DAWHXZ010000000
VERSION     DAWHXZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.200"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            49..2910
                     /locus_tag="WIE35_001801"
     CDS             49..2910
                     /locus_tag="WIE35_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEZ4154682.1"
                     /translation="MINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGY
                     NENYKNYTAYMRKEAPEDGDGKDIEASFDDEVVIETEAKPTDIRHVKEIGHIDVVSHI
                     IGGRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFG
                     TTSRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNML
                     FIFSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLS
                     APYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIG
                     AVGVDSKFGWGLLDTGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKG
                     GSQLQLHGNNTYTGKTVIEGGSLVLYGNNKSDMHVETKGALIYNGAASGGSLNSDGIV
                     YLADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAG
                     YLNRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRG
                     NAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMP
                     GIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLD
                     HNATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTW
                     SENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGT
                     LMQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGL
                     AGLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVA
                     GLGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT          827 a          873 c          880 g          574 t
ORIGIN      
        1 aatctgcata ccggagactt tacaaaccca aatgacgcat acaagaattt gatcaacctc
       61 aaacctgcaa ttgaagcagg ctatacagga cgcggggtag aggtaggtat cgtcgacaca
      121 ggcgaatccg tcggcagcat atcctttccc gaactgtatg gcagaaaaga acacggctat
      181 aacgaaaatt acaaaaacta tacggcgtat atgcggaagg aagcgcctga agacggagac
      241 ggtaaagaca ttgaagcttc tttcgacgat gaggtcgtta tagagactga agcaaagccg
      301 acggatatcc gccacgtaaa agaaatcgga cacatcgatg tggtctccca tattattggc
      361 gggcgttccg tggacggcag acctgcaggc ggtattgcgc ccgatgcgac gctacacata
      421 atgaatacgc atgatggaac caagaacgaa ataatgtctg cagccatccg caatgcatgg
      481 gtcaagctgg gcgaacgtgg cgtgcgcatc gtcaataaca gttttggaac aacatcgagg
      541 gcaggcactg ccgaccattt ccaaatagcc aattcggagg agcagtaccg ccaagcgttg
      601 ctcgcctatt ccggcggtga taaaacagac gagggtatcc gcctgatgca acagagcgat
      661 tacggcaact tgtcctacca catccgtaat aaaaacatgc ttttcatttt ttcggcaagc
      721 aatgacgcac aagctcagcc caacacactg accctattgc cattttatga aaaagacgct
      781 caaaaaggca ttatcacagt tgcaggcgta gaccgcagtg gagaaaagtt caatggctcc
      841 aaccattgcg gaattactgc catgtggtgc ctgtcggcac cctatgaagc aagcgtccgt
      901 ttcacccgta caaacccgat tcaaattgcc ggaacatcct tttccgcacc catcgtaacc
      961 ggcacggcgg ctctgctgct gcagaaatac ccgtggatga gcaacgacaa cctgcgtacc
     1021 acgctgctga caacggctca ggacatcggt gcagtcggcg tggacagcaa gttcggctgg
     1081 ggactgctgg atacgggtaa ggccatgaac ggacccgcgt cctttccgtt cggcgacttt
     1141 accgccgata cgaaaggtac atccgatatt gcctactcct tccgtaacga catttcaggc
     1201 acgggcggcc tgatcaaaaa aggcggcagc caactgcaac tgcacggcaa caacacctat
     1261 acgggcaaaa ccgttatcga aggcggttcg ctggtgttgt acggcaacaa caaatcggat
     1321 atgcacgtcg aaaccaaagg tgcgctgatt tataacgggg cggcatccgg cggtagcctg
     1381 aacagcgacg gcattgtcta tctggcagat accgaccgat ccggcgcaaa cgaaaccgtg
     1441 cacatcaaag gcgatctgca gctgggcggc gaaggtacgc tgtacacacg tttgggcaaa
     1501 ctgctgaaag tggacggtac ggcgatgacc ggcggcaagc tgtacatgtc ggcacgcggc
     1561 aaaggggcag gctatctcaa ccgtaccgga caacgtgttc ccttcctgag tgccgccaaa
     1621 atcgggcggg attattcttt cttcacaaac atcgaaaccg acggtggtct gctggcttcc
     1681 ctcgacagcg tcgaaaaaac agcgggcagt gaaggcgaca cgctgtccta ttatgtccgt
     1741 cgcggcaatg cggcacggac tgcttcggca gcggcacatt ccgcgcccgc cggtctgaaa
     1801 cacgccgtag aacagggcgg cagcaatctg gaaaacctga tggtcgaact ggatgcctcc
     1861 gaatcatccg caacacccga gacggttgaa actgcggccg ccgaccgcac agatatgccg
     1921 ggcatccgcc cctacggcgc aactttccgc gcagcggcag ccgtacagca tgcgaatgcc
     1981 gccgacggtg tacgcatctt caacagtctc gccgctaccg tctatgccga cagtaccgcc
     2041 gcccatgccg atatgcaggg acgccggctg aaagccgtat cggacgggtt ggaccacaac
     2101 gctacgggtc tgcgcgtcat cgcgcaaacc caacaggacg gtggaacgtg ggaacagggc
     2161 ggtgttgaag gcaaaatgcg cggcagtacc caaaccgtcg gcattgccgc gaaaaccggc
     2221 gaaaatacga cagcagccgc cacactgggc atgggacaca gcacatggag cgaaaacagt
     2281 gcaaatgcaa aaaccgacag cattagtctg tttgcaggca tacggcacga tgcgggcgat
     2341 atcggctatc tcaaaggcct gttctcctac ggacgctaca aaaacagcat cagccgcagc
     2401 accggtgcgg acgaacatgc ggaaggcagc gtcaacggca cgctgatgca gctgggcgca
     2461 ctgggcggtg tcaacgttcc gtttgccgca acgggagatt tgacggtcga aggcggtctg
     2521 cgctacgacc tgctcaaaca ggatgcattc gccgaaaaag gcagtgcttt gggctggagc
     2581 ggcaacagcc tcactgaagg cacactggtc ggactcgcgg gtctgaagct gtcgcaaccc
     2641 ttgagcgata aagccgtcct gtttgcaacg gcgggcgtgg aacgcgacct gaacggacgc
     2701 gactacacgg taacgggcgg ctttaccggc gcgactgcag caaccggcaa gacgggggca
     2761 cgcaatatgc cgcacacccg cctggttgcc ggtctgggcg cggatgtcga attcggcaac
     2821 ggctggaacg gcttggcacg ttacagctac gccggttcca aacagtacgg caaccacagc
     2881 ggacgagtcg gcgtaggcta ccggttctga cggacaggaa gcagacagcc gcaaagatca
     2941 cggtctttgc ggctgtttct tatgaaaaga aaaccctatt ccaattgcct gcttctattg
     3001 tttcaagact tcttccaaag attcggcatt aatcagatgt atagcggatt aacaaaaatc
     3061 aggacaaggc ggcgaagccg cggacagtac aaatagtacg gaaccgattc actcggtgct
     3121 tgagcacctt agagaatcgt ttctttgagc taag
//
LOCUS       DAWHXZ010000201         3151 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000201 DAWHXZ010000000
VERSION     DAWHXZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.201"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(125..976)
                     /locus_tag="WIE35_001802"
     CDS             complement(125..976)
                     /locus_tag="WIE35_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ4154683.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
     gene            1324..2838
                     /gene="katA"
                     /locus_tag="WIE35_001803"
     CDS             1324..2838
                     /gene="katA"
                     /locus_tag="WIE35_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ4154684.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            2914..3135
                     /locus_tag="WIE35_001804"
     CDS             2914..3135
                     /locus_tag="WIE35_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154685.1"
                     /translation="MPSETSFDRFRRHFPIPPPPFQWAFFIKRKISRHSRPTAHFSAV
                     IPAPSPPVQTGTKTFPRHSRESGNLERKI"
BASE COUNT          759 a          889 c          814 g          689 t
ORIGIN      
        1 gtttctagat tccctctttc gcgggaatga cgggatgcag gtttccgcgc ggacggattc
       61 ggcattcccg ctttcgcaga aatgccggct tttggtttgt tgtctttata agattattcg
      121 gcaattattc tgcggttttg cgtttgtgtg ctgccggaat ctcaaaggat tctctgtatt
      181 tggcaatggt gcggcgagaa acctccatgc cgcggaaagc cagcaggttg gcgagcgcct
      241 cgtcagaata cggcgtgggt gtggacgagg caaaaccggc ggcgggaaag ggattgagcg
      301 aagcaatgag gtcgagtgcg gcttcgagtg tgccgctgtc ggtttggggc aggcgttttt
      361 ttattcgcgc gagggtttgg ctgcggttgc cgtcaatgct gtcgagggcg tttcggacga
      421 tatgcagggc ggagggtttg gcagcacatt cgcccgatct ttctatctgc agtatcagcg
      481 attcgttcaa atcggcggcg gccacgcctg ccgggtcgaa ttttttcaat gcggtcaggg
      541 cttgtttcag cattgcttca tccaacatcc actccaaggg cgtatggtcg aggatgtctt
      601 cgatgctgtc ggtcagataa ccttgctcgt caaggaaatc gatgaggatg tggacacagg
      661 cggattcttg gtcggaaagc gggtgttcgc atacttgcgc gtgcaggtat tgcttgaaat
      721 cctgctcgcc ggcgatgttg gacagcatat cttcgccttc gtctccgccg atttgacggg
      781 caggcgcagt gtaatggctg aactcggcat cggaaaattc atccgtgtct ttgcgttcga
      841 gcagggggtt gtccgacagc cagttttcga cctcgcgttc aagttcgata cccgacatct
      901 gcaatacgcg caaagattgt tgcagccgct ggttgagctg ctgggtctgt ttgagcttta
      961 ttccgagtaa ggtcatgata atgtggggaa aattgttatt ttcagcctgt cggcgcaaaa
     1021 aatgccgcaa agcgtcattg cattataaat ggttttaatg agcgggttcg gattccgttc
     1081 gataacaaaa aacaaacgaa aatcaagaac cgattgctta taataatatt aaatcgattt
     1141 catagtttta atagcgaaaa tcttggcgta tagtcgcatc catagttttt acaaaaggga
     1201 aataaaatgt cgattcaaga aatttattgc aatcaagaaa ccggttacga atacgctttc
     1261 cgccaaatcg tactgtaagc cgtttacccc catttgacca acctgagaaa aggaacaaga
     1321 gcgatgacta cctccaaatg ccctgtaacc catctgacca tgaacaacgg cgcgcctgtt
     1381 gccgacaatc aaaacagcct gaccgccggt cctcgcggcc ctctgctggc gcaggatttg
     1441 tggctgaatg aaaaactcgc cgacttcgtg cgcgaagtca tccccgaacg ccgtatgcac
     1501 gccaaaggtt cgggcgcgtt cggtacgttt accgtaacgc acgacatcac caaatacacc
     1561 cgcgccaaaa tcttcagcga agtcggcaaa aaaaccgaga tgttcgcccg tttcaccacc
     1621 gtggcaggcg aacgcggcgc agccgatgca gaacgcgaca tccgcggttt tgcgttgaag
     1681 ttctataccg aagaaggcaa ctgggatgtg gtcggcaaca acacgcccgt gttcttcctg
     1741 cgcgacccgc gtaagttccc cgacctgaac aaagccgtca aacgcgaccc gcgcaccaat
     1801 atgcgctctg ccacaaacaa ctgggacttc tggacgctgc tgcccgaagc actgcaccaa
     1861 gttaccatcg tgatgagcga ccgcggcatc cccgccggct accgccatat gcacggcttc
     1921 ggttcgcata cctacagctt ctggaacgaa gcaggcgagc gtttttgggt gaaattccat
     1981 ttccgcaccc aacaaggcat taaaaacctg accaacgaag aagccgccaa aatcatcgcc
     2041 gacgaccgcg aaagccatca gcgcgactta tacgaagcca tcgaacgcgg cgagtttccg
     2101 aaatggacga tgtacatcca agtcatgcct gaagcagacg cggaaaaagt accttatcat
     2161 ccgtttgact tgaccaaagt ttggccgaaa aaagactatc cgctgattga agtgggcgaa
     2221 ttcgagttga accgcaatcc cgaaaacttc ttcgccgatg tggaacaatc cgccttcgca
     2281 ccgagcaacc tcgttcccgg tatcggcgcg agcccagata aaatgttgca agcccgtttg
     2341 ttcaattacg ccgacgcgca acgctaccgc ttgggcgtga acttccgcca aatccctgtc
     2401 aaccgtccgc gttgccctgt tcacagcaac cagcgcgacg gacaaggacg cgccgacggc
     2461 aactacggca gcctgccgca ctacgaaccc aatagcttcg gccaatggca gcaacaaccc
     2521 gacttcgccg aaccgccttt gaaaatcaac ggcgacgcgg cgcactggga ctaccgccaa
     2581 gacgatgacg actatttcag ccaaccgcgc gccctgttca acctgatgaa cgacgcgcag
     2641 aaacaggcat tgttcggcaa caccgccgcc gcaatgggcg acgcgcccga cttcatcaaa
     2701 taccgccata tccgcaactg ctaccgttgc gatccggcat acggcgaagg cgtggcaaaa
     2761 gccctcggac tgactgtcga agacgcacaa gccgcccgcg cgaccgaccc cgcactcggc
     2821 cagcccggtt tgctgtaagg gggcattatg tggatggaaa ttgaagaaat cctgtcccgc
     2881 gccgtccgct accggaaata accgggcata aaaatgccgt ctgaaacgtc gtttgaccgc
     2941 ttcagacggc atttccccat cccgcccccg ccgtttcagt gggcgttttt tattaaacgc
     3001 aaaatatccc gtcattcccg ccccacagcc cacttttccg ccgtcattcc cgcaccatcc
     3061 ccaccggttc aaaccggcac gaaaactttt ccgcgtcatt cccgcgaaag cgggaatcta
     3121 gaacgtaaaa tctaaagaaa ccgtttttcc c
//
LOCUS       DAWHXZ010000202         3140 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000202 DAWHXZ010000000
VERSION     DAWHXZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.202"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..380)
                     /locus_tag="WIE35_001805"
     CDS             complement(<1..380)
                     /locus_tag="WIE35_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154686.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="WIE35_001806"
     CDS             complement(438..803)
                     /locus_tag="WIE35_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154687.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1330)
                     /locus_tag="WIE35_001807"
     CDS             complement(785..1330)
                     /locus_tag="WIE35_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154688.1"
                     /translation="MKKQITAAVMMLSMIAPAMANSMDNQIFENQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            complement(1493..1765)
                     /locus_tag="WIE35_001808"
     CDS             complement(1493..1765)
                     /locus_tag="WIE35_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154689.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIS
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
     gene            complement(2641..2889)
                     /locus_tag="WIE35_001809"
     CDS             complement(2641..2889)
                     /locus_tag="WIE35_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154690.1"
                     /translation="MNIKNEIVALISKKIDGGQYSSLENFALSVLREKIIKNTVSSDE
                     KLLIINDFLSGFLEFDPETGKPVGETLKIEQMIDFFLV"
BASE COUNT         1013 a          681 c          452 g          994 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcgccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccctctcag tctccttcat ctccttttga gaaagttccg
     1201 ccaactgcat cggcgcatct gcccgcgtat ggaatacttg gttttcaaat atctgattgt
     1261 ccatgctgtt tgccattgcg ggggcaatca tagacagcat cattacggct gcggtaattt
     1321 gttttttcat aataactcct ttggattaca aggttggaaa atcaaagccc tgcttagaac
     1381 gtatgttgca cacccaattt caataggtaa atcagattgc aaatccagca atttgaatat
     1441 tgtcattgtt ccgtgcaaaa gggattttta ttgatgagtt gtgtactggg tttcagcttg
     1501 gctttttaga taatcttttc acaagaacat acatcaatac tgtccaaaaa aaaagatttg
     1561 ataaatatat tgcaacatat tttattccac ctccatccga aacagaaacc aatatatttt
     1621 taattaatac atagctgatt attacattta ttattcctat aatatataag gatcttgcca
     1681 aaattttttt tgattttatt ttaggaatat ctcttatcca tgctaaaata cagcccaatg
     1741 tcgaaaagaa aataacagca aacactacct tacctcctct cacttccaac caaaccgata
     1801 gcaggtttgg ttggaagttg gtttatttat tatttaacgg cgaatgtcag tgttcttacc
     1861 cgtagaacct gcataaccat ttatgccacc agcaacagtc cccactgcta cacctcgcat
     1921 accattaatc agaattcttc ctattttttc gaatccacta tttcttccaa agatgcagca
     1981 aacgcaggtt aagcctagac ccaatctacg tttgctgccg catctgatcc ctattgtttc
     2041 ttatccttac atcttcctgc cttgtcaatc aaataaagac agaagactat acaaaaactg
     2101 accgacatac tgaaaatacc tatccccttc catgcaatct ccgcaccatt gacccaatag
     2161 atcagactga gaaacaacaa agccacagta taaatcaacg taaaaaatat tgcgtaaagc
     2221 acaataaagg gaacttttat ctcacggttg ttttttataa tacattcagc aacttgattt
     2281 ccgaatatac ctgataaaaa atatagtatt agatcatcca tttcgtttat cttctatgtt
     2341 ttcccattgc ggcgctagaa gactgattta atgctgcacc atgcaatata gtgaattaac
     2401 aaaaaccagt acggcgttgc ttcgccttag ctcaaagaga acgattctct aaggtgttga
     2461 agcaccgagt gaatcggttc cgtactatct gtactgtctg cggcttcgtc gccttgtcct
     2521 gatttttgtt aatctactag agtttaaaca actttatttt tgattttatc ccaagtataa
     2581 acctgacaat cgtatttaaa agccggatat aagcctagct tgttcactgg tatgtatgat
     2641 ttagactaaa aagaaatcaa tcatttgctc aattttaagc gtctccccga caggtttccc
     2701 ggtctcagga tcaaactcaa gaaaacctga taaaaaatca ttaataatca ataatttttc
     2761 atcactggaa acagtatttt ttataatttt ttcccttagt acggataaag caaaattttc
     2821 taaagaacta tattgccccc catcaatttt tttagaaatt aaagcaacta tttcattttt
     2881 aatattcata aattaccctt aaatgataaa gggagcgaag cccgcttaaa acgtataact
     2941 gtatacccaa tttcagcaag cacaggttga gttgccaagc ctaacagagc ctgaatgatt
     3001 ggttataact caaccttata tctacactta ccgaaaacct caaataactt aataaaaatc
     3061 acacctaata cttactgaaa cgataaacta cattttcttt aaataaggtt ttatctgtta
     3121 tctcatttaa agacaataaa
//
LOCUS       DAWHXZ010000203         3135 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000203 DAWHXZ010000000
VERSION     DAWHXZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.203"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            113..2881
                     /locus_tag="WIE35_001810"
     CDS             113..2881
                     /locus_tag="WIE35_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ4154691.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRTGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQQYFVQTGGLKFNSNSGKWER
                     DLQRQFWKTSYYKQYKDPQKLKKYIEDQDKDWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYREHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEEVTCNDDKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
     gene            2911..>3135
                     /locus_tag="WIE35_001811"
     CDS             2911..>3135
                     /locus_tag="WIE35_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154692.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPRRRESRPFGFGF"
BASE COUNT          878 a          782 c          762 g          713 t
ORIGIN      
        1 ccaattgaaa tcttcagacg gtatatcgaa tttacacttt ttaatgttta tgccgcctaa
       61 aaaaatgcta atatatttct taattgtctg actgtttatt gttgaggaaa atatgagatc
      121 ttctttccgg ttgaagccga tttgttttta tcttatgggt gttatgctat atcatcatag
      181 ttatgccgaa gatgcagggc gcacgggcag cgaggcgcag atacaggttt tggaagatgt
      241 gcacgtcaag gcgaagcgcg taccgaaaga caaaaaagtg tttaccgatg cgcgtgccgt
      301 atcgacccgt caggatatat tcaaatccag cgaaaacctc gacaacatcg tacgcagcat
      361 acccggtgcg tttacacagc aagataaaag ctcgggcatt gtgtctttga atattcgcgg
      421 cgacagcggg ttcgggcggg tcaatacgat ggtggacggt atcacgcaga ccttttattc
      481 gacttctacc gatgcgggca gggcaggcgg ttcatctcaa ttcggtgcat ctgtcgacag
      541 caattttatt gccggactgg atgtcgtcaa aggcagcttc agcggctcgg caggcatcaa
      601 cagccttgcc ggttcggcga atctgcggac tttaggcgtg gatgacgtcg ttcagggcaa
      661 taatacctac ggcctgctgc taaaaggtct gaccggcacc aattcaacca aaggtaatgc
      721 gatggcggcg ataggtgcgc gcaaatggct ggaaagcgga gcatctgtcg gtgtgcttta
      781 cgggcacagc aggcgcagcg tggcgcaaaa ttaccgcgtg ggcggcggcg ggcagcacat
      841 cggaaatttt ggcgcggaat atctggaacg gcgcaagcag caatattttg tacaaacagg
      901 cgggttgaaa ttcaattcca acagcggaaa atgggaacgc gatttgcaaa ggcaattctg
      961 gaaaaccagt tactataaac aatacaaaga cccccagaaa ctgaaaaaat acatcgaaga
     1021 ccaagataaa gactggcggg aaaacctggc gccgcaatac gacatcaccc ccatcgatcc
     1081 gtccagcctg aagcagcagt cggcaggcaa tctgtttaaa ttggaatacg acggcgtatt
     1141 caataaatac acggcgcaat ttcgcgattt aaacaccaaa atcggcagcc gcaaaatcat
     1201 caaccgcaat tatcagttca attacggttt gtctttgaac ccgtatacca acctcaatct
     1261 gaccgcagcc tacaattcgg gcaggcagaa atatccgaaa gggtcgaagt ttacaggctg
     1321 ggggctttta aaggattttg aaacctacaa caacgcgaaa atcctcgacc tcaacaacac
     1381 cgccaccttc cggctgcccc gcgaaaccga gttgcaaacc actttgggct tcaattattt
     1441 ccacaacgaa tacggcaaaa accgctttcc tgaagaattg gggctgtttt tcgacggtcc
     1501 tgatcaggac aacgggcttt attcctattt ggggcggttt aagggcgata aagggctgct
     1561 gccccaaaaa tcaaccattg tccaaccggc cggcagccaa tatttcaaca cgttctactt
     1621 cgatgccgcg ctcaaaaaag acatttaccg cttaaactac agcaccaata ccgtcggcta
     1681 ccgtttcggc ggcgaatata cgggctatta cagctcggaa ggcgaattta agcgggcatt
     1741 cggagaaaac tcgccgacat acagggaaca ttgcaacccg agctgcgggc tttatgaacc
     1801 cgtattgaaa aaatacggca aaaagcgcgc caacaaccat tcggtcagca ttagtgcgga
     1861 cttcggcgat tatttcatgc cgttcgccag ctattcgcgc acacaccgta tgcccaacat
     1921 ccaagaaatg tatttttccc aaatcggcga ctccggcgtt cacaccgcct taaaaccaga
     1981 gcgcgcaaac acttggcaat ttggcttcaa tacctataaa aaaggactgt taaaacaaga
     2041 tgatacatta ggattaaaac tggtcggcta ccgcagccgc atcgacaact acatccacaa
     2101 cgtttacggg aaatggtggg atttgaacgg ggatattccg agctgggtca gcagcaccgg
     2161 gcttgcctac accatccaac atcgcaattt caaagacaaa gtgcacaaac acggttttga
     2221 gttggagctg aattacgatt atgggcgttt tttcaccaac ctttcttacg cctatcaaaa
     2281 aagcacgcaa ccgaccaact tcagcgatgc gagcgaatcg cccaacaatg cgtccaaaga
     2341 agaccaactc aaacaaggtt atgggttgag cagggtttcc gccctgccgc gagattacgg
     2401 acgtttggaa gtcggtacgc gctggttggg caacaaactg actttgggcg gcgcgatgcg
     2461 ctatttcggc aagagcatcc gcgcgacggc tgaagaacgc tatatcgacg gcaccaacgg
     2521 gggaaatacc agcaatgtcc ggcaactggg caagcgttcc atcaaacaaa ccgaaaccct
     2581 tgcccgccag cctttgattt ttgattttta cgccgcttac gagccgaaga aaaaccttat
     2641 tttccgcgcc gaagtcaaaa atctgttcga caggcgttat atcgatccgc tcgatgcggg
     2701 caatgatgcg gcaacgcagc gttattacag ctcgttcgac ccgaaagaca aggacgaaga
     2761 agtaacgtgt aatgatgata aaacgttgtg caacggcaaa tacggcggca caagcaaaag
     2821 cgtattgacc aattttgcac gcggacgcac ctttttgatg acgatgagct acaagtttta
     2881 aaggcagccc gcgttttgta gaaaaccgca atgccgtctg aaagcccttc agacggcatt
     2941 tgtttcccca aacgcatcat cctgccgcaa gcctatgcca atccgtttta tcgcatcggc
     3001 aactcaaaga aaaatccatt tcattcccac gcagggaagc cggtttttga tttcggttat
     3061 ttttggttgt ttcgggtaat ttatgagtcg tcattcccgc gcaggcggga atctagaccg
     3121 ttcggtttcg gtttt
//
LOCUS       DAWHXZ010000204         3101 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000204 DAWHXZ010000000
VERSION     DAWHXZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.204"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..365
                     /locus_tag="WIE35_001812"
     CDS             <1..365
                     /locus_tag="WIE35_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ4154693.1"
                     /translation="TDKGYLVLEHPKQKIGGRRIKDESLPKGYNIVSGKKSFEINKIL
                     DPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSFDIPKKDKYDLLGN
                     TVAVPVIKAVSERLLHTL"
     gene            381..1112
                     /locus_tag="WIE35_001813"
     CDS             381..1112
                     /locus_tag="WIE35_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ4154694.1"
                     /translation="MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            1146..2216
                     /gene="leuB"
                     /locus_tag="WIE35_001814"
     CDS             1146..2216
                     /gene="leuB"
                     /locus_tag="WIE35_001814"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ4154695.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDISYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAILAAL"
     gene            2379..2942
                     /locus_tag="WIE35_001815"
     CDS             2379..2942
                     /locus_tag="WIE35_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ4154696.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT          920 a          818 c          671 g          692 t
ORIGIN      
        1 tgacagataa aggctatttg gttttggagc atccgaaaca aaaaattggc gggcgaagaa
       61 tcaaagatga atccctgccc aaaggctaca atattgtttc aggaaaaaaa tcatttgaaa
      121 tcaataaaat attagaccca aatgatgttg cgccaacctt agttgcaatg gatatggagc
      181 accttttcgt cgttgacaac ggcggtttgc gcacacttac cggaaaagaa ggtttacgct
      241 tattcggcta tccggacgat tattcgtttg atattcctaa aaaagacaaa tacgatttat
      301 tgggcaatac cgttgccgtt cctgtgatta aggcggtatc tgaaaggctt ctgcatactt
      361 tataaaccga tagggataaa atggtaaaac ttactgcaca acaaatattt gataagttgc
      421 tgaatgagga aaaaatctta tcggtcaatg gtcaaattag atttttcttg ggcgatgtgg
      481 atattatcgt caaacaaaaa gatgtcgtcg gcaacatcat tcaagaatgg ctcggcggat
      541 ggttgagaaa aagggaaatt gaatttgatg tttcaaccaa tacccaaatg ccccccgatt
      601 tctttttaaa taaaaaagac cgcagtagag aattgttgga agtaaaggcg ttcaaccgaa
      661 atgccggccc gggttttgat attgcagatt ttaaaatgta ttctgatgaa atcattcata
      721 aaccctatat gctggatgta gattatttaa tattcggtta cgatatggac gacaacggca
      781 atgtaaccat caaggattta tggcttaaaa aagtatggca aattacgaga agcatggatg
      841 ggtgggcaat taatcttcaa gtcaaaaaag gcgtggtgca taaaatccgc ccgggtgttt
      901 ggtacagcat aaataaaaag aatatgccta tgtttgaatg cttggaagat tttgtttccg
      961 caattgaaga aaccgtttat caaaacccgg caacacggca taacgcatcc ttatggaaaa
     1021 agaaatttga ggaagcctat aaaaaacact acaaccgatc gatttccata cctcgttggc
     1081 acgaaattgc acacaaatac aaaaagaaat aacccttcaa ccatcaaccc ataaggaaac
     1141 ttgaaatgac caaacatatc gccatcctcc gaggtgacgg catcggtccc gaaatcgtcg
     1201 ccgaaaccgt ccgcgtactc gacaaactca tcgcccaagg cttggacatc agctacgaat
     1261 acgccccatt gggtggtgaa gcctacgacg aatacggcca tccttatccc gaatttacgc
     1321 aaaacctctg ccgcaaagcc gatgccgtcc tgcttggtgc agtcggttct cctcaatacg
     1381 acaacctcga ccgtccgttg cgccctgagc gcggcctgtt ggcaatccgt aaggatttga
     1441 acctgtttgc caatttgcgt cctgctgttt tatatcccga attagccaac gcctccacgc
     1501 tgaagcctga aatcgttgcc ggtttggata ttttgattgt gcgcgaactc accggcgaca
     1561 tctatttcgg cgaaccgcgc ggcatccgcg ttctggaaaa cggcgaacgc gaaggctaca
     1621 acaccatgaa atacagcgaa agcgaaatcc gccgtattgc ccatgttgcc ttccaatccg
     1681 cccaaaaacg cagcaaaaaa gtctgttccg taggcaaagc caacgttttg gaaaccaccg
     1741 aactgtggcg cgaaatcttt gaagaaatcg gcaaacaata tcccgatgtc gaactctccc
     1801 atatgtatgt ggacaacgcc gctatgcagc tcgtgcgcgc gcccaaacaa tttgacgtga
     1861 ttgccaccgg caacatcttc ggcgatattt tatcggacga ggcctctatg ctgaccggct
     1921 ccatcggtat gctgccttct gcttctttgg acgaaaacgg caaaggcctg tacgaaccgt
     1981 ctcacggctc cgctcccgac atcgccggac aaaacaaagc caacccgctg gccaccatcc
     2041 tctcgctcgc aatgttgctg cgttacagcc tgaacgacga agcgcgcgcg caacaagttg
     2101 aaaacgccgt ccaaaaagtg ctgcaacaag gcttgcgtac cagcgacatt tacgaagaag
     2161 gcacaaaact cgtttcctgc tccgaaatgg gcgatgcgat actcgccgcc ttgtaaaaaa
     2221 acaaagcgcg gatcacaccg cgctttttca tctgcccttt tccgcccgca ccgttcgtca
     2281 accgcctgca aacccaccgt ttaccggcag gaaatgccat aaatccccgc ctatgttagg
     2341 ataaacacct ttatcggccc acacaaagga ttatcatcat gaaaaaaatc atcttcgccg
     2401 cactcgcagc ggcagccgtt ggcactgcct ccgccgccac ctacaaagtg gacgaatatc
     2461 acgccaacgc ccgtttcgcc atcgaccatt tcaacaccag caccaacgtc ggcggttttt
     2521 acggtctgac cggttccgtc gagttcgacc aagcaaaacg cgacggtaaa atcgacatca
     2581 ccatccccgt tgccaacctg caaagcggtt cgcaacactt taccgaccac ctgaaatcag
     2641 ccgacatctt cgatgccgcc caatatccgg acatccgctt tgtttccacc aaattcaact
     2701 tcaacggcaa aaaactggtt tccgttgacg gcaacctgac catgcacggc aaaaccgccc
     2761 ccgtcaaact caaagccgaa aaattcaact gctaccaaag cccgatggcg aaaaccgaag
     2821 tttgcggcgg cgacttcagc accaccatcg accgcaccaa atggggcgtg gactacctcg
     2881 ttaacgttgg tatgaccaaa agcgtccgca tcgacatcca aatcgaggca gccaaacaat
     2941 aaatcttgat gcgaatcagc acaaatgccg tcttcatcaa accgccccgc caacggtaag
     3001 ccgatatggc ggagcacaat ccgccgaaag atatttcagc ggcaggcagg gcttgccgga
     3061 aagtccggaa agtatagtgg attaacaaaa accagtacgg c
//
LOCUS       DAWHXZ010000205         3091 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000205 DAWHXZ010000000
VERSION     DAWHXZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.205"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1385
                     /locus_tag="WIE35_001816"
     CDS             <1..1385
                     /locus_tag="WIE35_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HEZ4154697.1"
                     /translation="EAKGQGNPRKGGKAEKDTVSANKKVRSDKNGKAVKQDKKYREEK
                     NAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVSNSLKQLQEERIRQERIRQAR
                     GNLASVNRKQREAWDKFQKLNTELNRLKTEVAATKAQISRFVSGNYKNSQPNAVALFL
                     KNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKALAVQEQKINNELARLKKIQANVQ
                     SLLKKQGVTDAAEQTESRRQNAKIAKDARKLLEQKGNEQQLNKLLSNLEKKKAEHRIQ
                     DAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMSNLTAEDRNIQAPSVMGIGSADG
                     FSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVFYSTAPATVESIAPGTVSYADELDG
                     YGKVVVVDHGENYISIYAGLSEISVGKGYMVAAGSKIGSSGSLPDGEEGLYLQIRYQG
                     QVLNPSSWIR"
     gene            1512..2996
                     /locus_tag="WIE35_001817"
     CDS             1512..2996
                     /locus_tag="WIE35_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ4154698.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLINSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT          884 a          690 c          910 g          607 t
ORIGIN      
        1 aagaggcaaa aggacagggc aatccgcgca agggcggcaa ggcggaaaaa gacactgttt
       61 ctgcaaataa aaaagtccgt tccgacaaga acggcaaagc agtgaaacag gacaaaaaat
      121 acagggaaga gaaaaatgcc aaaaccgatt ccgacgaatt gaaagccgcc gttgccgctg
      181 ccaccaatga tgtcgaaaac aaaaaagccc tgctcaaaca aagcgaagga atgctgcttc
      241 atgtcagcaa ttccctcaaa cagcttcagg aagagcgtat ccgccaagag cgtatccgtc
      301 aggcgcgcgg caaccttgct tccgtcaacc gcaaacagcg cgaggcttgg gacaagttcc
      361 aaaaactcaa taccgagctg aaccgtttga aaacggaagt cgccgctacg aaagcgcaga
      421 tttcccgttt cgtatcgggg aactataaaa acagccagcc gaatgcggtt gccctgttcc
      481 tgaaaaacgc cgaaccgggt cagaaaaacc gctttttgcg ttatacgcgt tatgtaaacg
      541 cctccaatcg ggaagttgtc aaggatttgg aaaaacagca gaaggctttg gcggtacaag
      601 agcagaaaat caacaatgag cttgcccgtt tgaagaaaat tcaggcaaac gtgcaatctc
      661 tgctgaaaaa acagggtgta accgatgcgg cggaacagac ggaaagccgc agacagaatg
      721 ccaaaatcgc caaagatgcc cgaaaactgc tggaacagaa agggaacgag cagcagctga
      781 acaagctctt gagcaatttg gagaagaaaa aggccgaaca ccgcattcag gatgcggaag
      841 caaaaagaaa attggctgaa gccagactgg cggcagccga aaaagccaga aaagaagcgg
      901 cgcagcagaa ggctgaagca cgacgtgcgg aaatgtccaa cctgaccgcc gaagacagga
      961 acatccaagc gccttcggtt atgggtatcg gcagtgccga cggtttcagc cgcatgcaag
     1021 gacgtttgaa aaaaccggtt gacggtgtgc cgaccggact tttcgggcag aaccggagcg
     1081 gcggcgatgt ttggaaaggc gtgttctatt ccactgcacc ggcaacggtt gaaagcattg
     1141 cgccgggaac ggtaagctat gcggacgagt tggacggcta cggcaaagtg gtcgtggtcg
     1201 atcacggcga gaactacatc agcatctatg ccggtttgag cgaaatttcc gtcggcaagg
     1261 gttatatggt cgcggcagga agcaaaatcg gctcgagcgg gtcgctgccg gacggggaag
     1321 aggggcttta cctgcaaata cgttatcaag gtcaggtatt gaacccttcg agctggatac
     1381 gttgatcgga ttgccgaaac ggtaggcttt atgcaaatcg tgccgatatg gtgcgctgtt
     1441 ccgtgaagtt tgccgtttgc cgtacctgta ttttccggtt tttggaaatc aagagagaaa
     1501 gttaaagaag aatgtcgaaa cctgttttta agaaaatcgc actttatact ttgggtgcaa
     1561 tcagcggcgt ggccgtcagt ctggcggtgc agggttttgc cgccgagaag gacaggcggg
     1621 ataacgaagt cctgccggtg caatccatcc gcacaatggc ggaggtttac ggtcaaatca
     1681 aggcaaacta ctatcaggac aaacccgatg ccgatttgtt tgaaggtgcg atgaagggta
     1741 tggtggccgg tttggatccg cattccgaat atatggataa aaaaggttat gccgagataa
     1801 aggagtccac cagcggcgaa tttggcggct tggggatgga aatcgggcag gaagacggat
     1861 ttgtcaaagt ggtttcgccg attgaggaca cgcctgcgga acgggcgggg gtgaaaagcg
     1921 gcgatttcat tgtgaaaatc gataatgttt cgacacgcgg catgacggtc agcgaagcgg
     1981 tgaagaaaat gcggggcaag ccgggtacga agattacttt gacgctgtcg cgcaaaaatg
     2041 ccgacaagcc gatagtcgtc aacctgaccc gtgccattat taaagtgaaa agcgtccgcc
     2101 atcacctgat cgaacccgat tacggctata tccgcgtgtc gcagttccaa gagcggacgg
     2161 tcgaaagcgt caataccgcc gcaaaagagc tggtaaagga aaataaagga aaaccgctca
     2221 aggggctggt gttggatttg cgcgacgacc ccggcgggct tttgactggc gcggtcggcg
     2281 tgtcggcggc atttctgccg tctgaagcag tcgtcgtcag caccaaggga cgcgacggca
     2341 aagaccgcat ggtactgaaa gccattcctg aagattatgt gtacgggatg ggcggcgatt
     2401 cgttggcggg cattcctgcc gagttgaaaa ccataccgat gacggttctg attaattcgg
     2461 gttcggcttc cgcgtcggag attgtcgcag gtgcattgca ggatcataaa cgcgcggtca
     2521 tcgtcggtac gcagagcttc ggcaaaggtt cggttcagac tttgattcct ttgtccaacg
     2581 gcagcgcggt caagctgaca acggcactgt attatacgcc gaacgaccgt tctattcagg
     2641 cgcaggggat tgttcccgat gtcgaagtaa aagataagga acgcattttt gaaagccgcg
     2701 aggcggattt ggtcggacac atcggcaatc ctttgggcgg cgaggatgtg aacagtgaaa
     2761 cccttgccgt gccgcttgaa aaagatgcgg ataagcccgc tgtaaaagaa aaaggtaaaa
     2821 agaaaaagga cgaggatttg tcttcaaggc ggattcccaa ccctgccaaa gacgaccagt
     2881 tgcggaaagc tttggattta gtcaagtcgc ccgagcagtg gcagaagtct ttggggctgg
     2941 cggcgaaaaa gccggtttca aataaagata agaaagataa aaaagataag aagtaggttt
     3001 tgacgatgcc gtctgaaagg atttcagacg gcatattatg tttgaaagga caggttatga
     3061 gcgatttttt accggaattt gaaccgtgcg g
//
LOCUS       DAWHXZ010000206         3050 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000206 DAWHXZ010000000
VERSION     DAWHXZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.206"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(471..1007)
                     /locus_tag="WIE35_001818"
     CDS             complement(471..1007)
                     /locus_tag="WIE35_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ4154699.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYTTD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(1041..1397)
                     /gene="folB"
                     /locus_tag="WIE35_001819"
     CDS             complement(1041..1397)
                     /gene="folB"
                     /locus_tag="WIE35_001819"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ4154700.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1453..2055
                     /gene="plsY"
                     /locus_tag="WIE35_001820"
     CDS             1453..2055
                     /gene="plsY"
                     /locus_tag="WIE35_001820"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ4154701.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAVLTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGGSR"
     gene            2132..2977
                     /locus_tag="WIE35_001821"
     CDS             2132..2977
                     /locus_tag="WIE35_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ4154702.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAKNLPTELNGFIAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
BASE COUNT          754 a          927 c          651 g          718 t
ORIGIN      
        1 gagctaaggc gaggcaacgc catactggtt tttgttaatc cactataccg ttctcaaatt
       61 caaattgggc ttttaggtct ataacaaaga aaaagccttg gcaggataaa cctaccaagg
      121 ctttttatta aaaaaaacgc tctattactg tgtctaaaaa tgcaaaaaaa gtaaccatca
      181 taaccctttt gccaaaatca atatcatctt ccgatgaatg aattgacggt atataagaaa
      241 gaattaataa gctcataaac cggtaagaaa aatcagtaac aaagattttc attattaacc
      301 attcttaaaa atgatcacgt tcgtttgggt aattattata cccttttccc tcttattact
      361 aaagcacgaa tgactttaaa ggacgcgccc agagacaagc cgaaaaggca tatctcatcc
      421 gccaaacaaa atgcccgcca atctactccg gagggcagtt caacaccctg tcaatccttc
      481 atcagccagt attgcaaacc gattaatgtc ttgccgtctt taatttcatc gtttgccaat
      541 gcctgacgga cttcttcttt cgacatcaat acggtttccg taatctcgtc ttcgtcattg
      601 gcaagcgtac tgcccaaacg cacgccttcc gcctcgaaca gatacatttt ttcgttgcaa
      661 aaaccgaccg ccgtataaaa actgtaaagc aggcgtacgc tgtcggtggt ataaggcgtt
      721 tcttcagcca gttctcgtaa agcgcatgct gccatatcct cgcccgccac atccagcttg
      781 cccgcaggaa gttccaatgt cgcctgattt gccgcatacc gccactgccg taccaaaacg
      841 accttttcct catccgttac cgccaacacg cacgccgcgc ccggatgccg gatgacgata
      901 cgctgccctt cattgccgtt gggcaacctg accttatccc tgctgatgct gacgaaaccg
      961 ccctcgtaaa tggtttcgcc gcctaatttt acttccctca aatccacttc atatctcctt
     1021 tccgtttcct attctgccgt tcaatcttca cgcttgccgc gctcgatttc cacgccaacc
     1081 tcgcgcacgc cttccaaaat acccggcttg acgattttca cgcgcaccca caccgcgccg
     1141 aaatatccca aaaccaaatc ggcaatatat tccgccaacg cttccaaaag caggaaatcc
     1201 tgttctttca gatgtcggcg cagcgtttcg catacctcgg cataatgcac cgtattggca
     1261 atatcgtcgt ccgaacccgc tttctcggga acgccgatgt ccaaatcgac aatcagggtc
     1321 tgcaaccgtt cgcgctccca gccgtacacg ccgataagcg tatccgcctt gatgccgtgc
     1381 aaaaagattt tatccataat ccgccccgtt ttttgctaaa ataaccgcac cattctaagt
     1441 aaagtaccgc aaatgttcaa cataccggct gttgctgttt cctatctgat cggctcactt
     1501 tctttcgccg tcatcgtgtc caagtattac ggcatggacg acccgcgcac ctacggatcg
     1561 ggcaatccgg gggcaaccaa tgttttacgc agcggcaaaa aaaaggcggc cgtgctgacg
     1621 ctcttgggcg atgccgccaa aggtttggtt gccgttttgc ttgcacgcgt gcttcaagaa
     1681 ccgctcggtt tatccgacag cgcaatcgct gccgtcgcac tcgccgcgct ggtcggacat
     1741 atgtggccgg tgtttttcgg atttaaaggc ggcaaaggcg tggcaacggc attgggcgtg
     1801 cttctggcac tctctcccgc aactgccttg gtctgcgcgt tgatttggct tgtgatggca
     1861 ttcggcttca aggtgtcctc ccttgccgca ttaaccgcca caatcgccgc cccccttgcc
     1921 gcactgtttt ttatgccgca tacttcttgg attttcgcaa ccctcgcaat cgccatattg
     1981 gtgttgctcc gccataagag caacatcctc aacctgatta aaggcaaaga aagcaaaatc
     2041 ggcggcagcc gctgatttcc catccatccc tgtttatgcc gtctgaaccc cgcttcagac
     2101 ggcatccgaa tatcccgccg ttttaccgac tatgctcagt tacagacacg cattccacgc
     2161 cggcaaccac gccgatatgc tcaaacactt caccttgttt ttagtgctgc aatatttcaa
     2221 ccgcaaagac aagccctact ggtacatcga cacgcacggc ggcgcgggtg tgtacaatct
     2281 cgaaggcagc gaggcacaaa aagtcggcga ataccggcaa ggcatcgccc tacttcgaca
     2341 agcaaaaaac ctgccaaccg agttaaacgg ctttatcgcg cacatacaaa aaatcctgcc
     2401 ctcgcccgaa ctttactgcg gttctccgtg gctggcgcaa tcgctgaccc gcgtcggcga
     2461 caaattacgc ctgtttgagc tgcaccccac cgactttgtc catctgcaaa acaatatggg
     2521 cgaagcagga ttgggcaaac gcggacaagt gttgcgcgaa gacggttaca aagggctgat
     2581 ttccctgctg ccgcccccgc cgcgccgcgc caccgtcctc atcgacccgc cctatgagga
     2641 aaaacaggac taccggcgtg taacggaaac gctgaaagcc gctttaaaac ggtttgagtc
     2701 cggctgttat ctcatttggt atccctgcct cagccgcgaa gaaagccgca agctgcccga
     2761 agaattgaaa aaactcctgc ccgacaacta ccttcacgcc gaacttcacg tccacgcgcc
     2821 gaaagccgac ggtttcggta tgcacggcag cggaatgttc gtcatcaatc cgccctatct
     2881 gttggcggaa caattggcgg caaacctccc cgccctgacg cggctgctgg cgcaagacga
     2941 aggcgcgcgc taccttttgg acagcaaaat ccgctgaccc cgatgccgtc tgaaaccctt
     3001 cagacggcat ctcccatcaa acaaaaccgg acgatatacc ggcagccggc
//
LOCUS       DAWHXZ010000207         2995 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000207 DAWHXZ010000000
VERSION     DAWHXZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.207"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            82..243
                     /locus_tag="WIE35_001822"
     CDS             82..243
                     /locus_tag="WIE35_001822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154703.1"
                     /translation="MRWLNLKNSTSVHNTQGIRTKEAVEQGLRGNNRPEKGIGNRWNY
                     DGFIKELAK"
     gene            293..577
                     /locus_tag="WIE35_001823"
     CDS             293..577
                     /locus_tag="WIE35_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154704.1"
                     /translation="MKVITITLFTSCILSGCYGLEYGMSGGIPHDDWAIGSHVSQAKA
                     DAAWKECSDKYMRNGGSGLVEQCMNDKGLIRLSEIRRRQKRAETQRAVIK"
     gene            complement(702..1418)
                     /gene="trmB"
                     /locus_tag="WIE35_001824"
     CDS             complement(702..1418)
                     /gene="trmB"
                     /locus_tag="WIE35_001824"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ4154705.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDANFGSNHPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIEENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1540..2943)
                     /locus_tag="WIE35_001825"
     CDS             complement(1540..2943)
                     /locus_tag="WIE35_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ4154706.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLISLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYREAAAQGLAAAQWQLGLQYRFGQGTPTDTTQAINHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDKAVYRFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDICRYGLGIAPDTEKARHYYRQAAEAGSLTAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
BASE COUNT          627 a          621 c          902 g          845 t
ORIGIN      
        1 atacgattaa agtaatgtcg gattcaagaa tccgacctac ggaaaactaa agattttata
       61 cgggctacgg cttgctggct tatgcgttgg cttaatttga aaaacagcac atcggttcat
      121 aatacacaag gtattagaac aaaagaagca gttgaacagg gattgcgtgg taataataga
      181 cctgaaaaag gaataggtaa tagatggaat tatgatggat ttataaagga attagcgaag
      241 taacaggtat tccaaatatt tctgataaat ataaagaaca aggagtaaaa aaatgaaagt
      301 aatcactatt acattgttta ctagctgcat actatctgga tgctatgggc ttgaatatgg
      361 aatgagtggc ggcattcctc atgatgactg ggcaataggt agccatgtta gtcaagccaa
      421 agcagatgct gcgtggaaag aatgttctga caaatatatg agaaatggtg gttcaggatt
      481 agtagaacaa tgtatgaatg ataaaggatt aatacgttta tctgaaatac gtcgtaggca
      541 aaagagagca gaaacacaaa gagcagtaat taagtaacaa taatgaaata ctactgttcc
      601 cgaacaaagc ggcatttacg caggtgatga cggctatatt ttgaaaaatg ccgtctgtaa
      661 gcaaacgtgc ttcagacggc atttttttat ccggcggtct gttatccgat ccgtttgaat
      721 accaagtccc aaacgccgtg tccgaggcgt ttgccgcgcg cttcgaattt ggtttcgggg
      781 cggtagtccg gggtgggggc gtagtctgcc gccgtatttt gcaggctgtc gaagctgctg
      841 aggacttcaa gcatctgctg tgcatattct tcccagtctg tcgccaggtg gatatagccg
      901 ccggttttga gtttgggcag cagtttggcg atgaaggggg cttgtatcag acggcgtttg
      961 tggtggcgtt ttttgtgcca cgggtcgggg aagaatatgt ggatgccgtc gagcgagccg
     1021 tcttgcagca tattttcgac aacttctacg gcgtcgtgcc gcatcacgcg gatgttttcc
     1081 aaatggtttt cttctatgag tttgagcagg ttgcctacgc cgggaccgtg tacgtcgatg
     1141 gcgagaaagt cggtttcggg cagcctgcgg gcgatttctg cggttgctgt ccccataccg
     1201 aagcctattt cgaggatttt ggggtggttg cttccgaaat tggcatcaag atcggccggt
     1261 gttgcttggt aatcgatgcc gaagctgtcc cataaggtat cgatggcgcg ttgctgcgcg
     1321 gcggtcatat ggctttggcg gaggacgaaa ctgcggatgc tgcggggatg tccggcttga
     1381 cggtcgtttg gtgtttggtt ttcggcggtg tctgtcataa tgcttccgtg cgcttgtgca
     1441 atcaggatgc cgtctgaatg tgaaaggcct gttcggacgg cgggaaggga aaggggcgat
     1501 tatacctgtt tcgtttcggc agggtaggtg taacgctgtt cagacggcat tttgccattg
     1561 ttgcagcagt tcttgcagga cgcttttttg cccgtatccg ttgtttatgg cttcttgcag
     1621 gtagcggcag gctttttctt tgtccggccc gacaccgtgt ccgctgtaat ggatgcaggc
     1681 gaggttgtag aacgccatac tgtctttttt tgcggcggct ttttcaaacc atttgcgtgc
     1741 ttcattgtag tcggcggtca taccctgtcc gaagtaatac atgctgccca gattggtttg
     1801 ggctttgctg tgtccgagtt ctgcggcttc tgtgtagagt ttgagcgcgg ctgcgtattc
     1861 gggcgcgcat tgtaatccgt aatgcagggc ttgggctttt tgataaagct gttctgccct
     1921 ttgccgccta atggcggaat ctttaatgcc gccgtattgg tcaggatggt ttaacgcgct
     1981 gtcggatatg agtttctgat aggcggtaag gctgccggct tcggctgcct gccggtaata
     2041 atgccgggct ttttccgtat cgggggctat gcccaaaccg tagcggcaga tgtcgcccag
     2101 tatccgcaaa ccttcgggat ggcgttcggc ggcggctgct tcggcgtgat gcagggcaag
     2161 tttgtagttt ctttccggat gcttgccttg caggtagatg tcggcaagtg cggcatgggc
     2221 atcggggtca ttttcctgtg cggcctgttg aaaccggtaa acggctttat cgggagccgt
     2281 aggtaggatg agctcggcaa gcggggtgta ggcgggaatg tatccttgtt gtgcggcggc
     2341 gcgcaaatga ttgatagctt gggtcgtgtc ggttggtgta ccttgtccga aacggtattg
     2401 aaggccgagc tgccattgtg ccgccgccaa gccttgtgct gcagcctcgc ggtagagcag
     2461 gtgtgccgtt tcaaaatccg gcggcgtgcc gtaccggcag tattgggcaa gcaggtattg
     2521 cccgtggggg ttgttgtttt ttgcgcttgt ttttgcccat ttcagggctt cttcaaaatg
     2581 tcgggacgca tacagcaggt acatcaggcg gacttgtgct tcaggcagcc cttcctctgc
     2641 taatggtctg taaagcgata tcagggtttc cgggggtgcg ccttccaatt cttggatata
     2701 tgccagattg taacgcgcgt agggatgtcc ctgttcggcg gcgatgcgga gcatttccat
     2761 accttgttcg cggtcttggt aggggctgtc cgcgtccatc aggtgttttg ccaactcgaa
     2821 tgcggcggcg ggatgacctg ccgaggccgc ttctgtcagc agtcggcgga cttcggtaaa
     2881 gttatattgt ttttgagaca ggcgttctaa ggcctgccga tagggtaagt cgggattgtt
     2941 catatcagat cgttttccgt tttgtcgaat catcgttatt gtgttatgaa gttag
//
LOCUS       DAWHXZ010000208         2989 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000208 DAWHXZ010000000
VERSION     DAWHXZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.208"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..602
                     /locus_tag="WIE35_001826"
     CDS             <1..602
                     /locus_tag="WIE35_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ4154707.1"
                     /translation="NAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLAVVRDLSER
                     GISSPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLSAGSSWTDE
                     YGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALKFYAKLRET
                     SAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(716..1672)
                     /locus_tag="WIE35_001827"
     CDS             complement(716..1672)
                     /locus_tag="WIE35_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4154708.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     AFIESGSTVQAAMHFCGYRHAGRLNEAFRRHYGFLPSDAKKC"
     gene            1908..2873
                     /locus_tag="WIE35_001828"
     CDS             1908..2873
                     /locus_tag="WIE35_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4154709.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKE"
BASE COUNT          634 a          831 c          926 g          598 t
ORIGIN      
        1 gcaatgcctt cgtgttggcg aacatacgcg gcggcggcga gttcggcccg cgctggcatc
       61 aggcggcgca gggcatcagc aaacacaaaa gcgtcgatga tttgcttgcc gtcgtgcgcg
      121 atttgtccga acgcggcatc agttcgcccg aacacatcgg tttgcagggc ggcagcaacg
      181 gcggactgat taccgccgcc gccttcgtgc gcgaaccgca aagcatcggc gcgctggtgt
      241 gcgaagtgcc gctgaccgat atgatccgtt atccgctgct ctccgccggt tcaagctgga
      301 cagacgaata cggcaatccg caaaaatacg aagtctgcaa acgccggctg ggcgaattgt
      361 cgccgtatca caatctttca gacggcatcg attatccgcc tgcgctcatc accaccagcc
      421 tcagcgacga ccgcgtccat cccgcccacg cgctcaagtt ctacgccaaa ctgcgtgaaa
      481 cctccgcgca atcttggctc tactctccgg acggcggcgg ccataccggc aacggcaccc
      541 aacgcgaatc cgccgacgaa ctcgcctgcg tgttgctgtt tttgaaagag tttttgggct
      601 aagggcgggg agcggcactg ccgccgcgaa tgaaaaaagg tcgtctgaaa ctgctttttc
      661 agacgacctt ttttaatggt tgtttcaaat caaaatatct atgccgccgg ccccatcagc
      721 acttcttcgc atccgaaggc aaaaatccgt aatgccgcct gaacgcttca ttgagccgtc
      781 ccgcgtggcg gtagccgcaa aagtgcatgg cggcttggac ggtgctgccg gattcgatga
      841 aggcgagcgc gtgttccagc cgcaggcggc gcaggcatcc ggcgacggtt tcgccggttt
      901 gcgctttgaa atagcgtttc aggtagcatt cgttcagtcc gacgcggcgg gcgatttcgg
      961 cgatggtcag cggacgggcg aattcgtgtt gcaggatgtc ggcggcttcg tctatgcgcc
     1021 gacggcggta accgttgtcg tggcggcgga aggtgaagcg caataatcgg gcggagagtt
     1081 ccagcgcggc ggcttcgtcg gcaagcaggc cgaagccgtc cgattcgaac gggcgttgca
     1141 gcagtgggca ggccgccgcc gtcagtgccg ctgcgttttg cgccagccgt tgcagggcga
     1201 atcggcctat cgtttgcggc gaaaacaggc gttcgtccag caagccttcg tcgtgccagc
     1261 ggcgcagttt ttccagcgaa aaatccaaat gcagcgcgca catgccgctg ttgtcgggca
     1321 gcagggtttc ggatacgtcc gccaactcgc cgcgtaccag ccagatttcg ccggcagacg
     1381 ggcggtattc cctgccgccc atttgtaacc ggttctgccc cgacaccatg acgaacaagg
     1441 cgcagttgtg gctgaaattg tggatttcgg tgggaaacgc gcccgttccg ccgccgcgca
     1501 tccgcgacaa ggtgatgccc gagtcgaagc ggttgatgca catttccaga tgcaaaccgg
     1561 gctgttttgc ctgcgcaatg agcgcgctgt cggaacagcc gtccaacgcc cagccggatt
     1621 tatcggagcg gacataggtt tggtactggc ggtagatggc ggcggtgttc atgattggat
     1681 aggaacgagt tgtctaacaa atgaattaaa taggaattat taccaataat caagcgcagg
     1741 gattggttga aacggaaaag gtcgtctgaa agggtgtttc agacgacctt ttccgtatcg
     1801 ggaatttgtt ttgccgtatc gggaattttg cgttttgcgg cgtggtttct gcaggttgtt
     1861 tgcttaataa taaacattct tattcgtatg caaaggaacc gcacaccgtg aaaccgcgtt
     1921 tttattgggc agcctgcgcc gtcctgctga ccgcctgttc gcccgaacct gccgccgaaa
     1981 aaactgtatc cgccgcatcc gcatctgccg ccacactgac cgtgccgacc gcgcggggcg
     2041 atgccgttgt gccgaagaat cccgaacgcg tcgccgtgta cgactgggcg gcgttggata
     2101 cgctgaccga attgggcgtg aatgtgggcg caaccaccgc gccggtgcgc gtggattatt
     2161 tgcagcctgc atttgacaag gcggcaacgg tggggacgct gttcgagccc gattacgaag
     2221 ccctgcaccg ctacaatcct cagcttgtca ttaccggcgg gccgggcgcg gaagcatatg
     2281 aacagttggc gaaaaacgcg accaccatag atctgacggt ggacaacggc aatatccgca
     2341 ccagcggcga aaagcagatg gagaccttgg cgcggatttt cggcaaggaa gcgcgcgcgg
     2401 cggaattgaa ggcgcagatt gacgcgctgt tcgcccaaac gcgcgaagcc gccaaaggca
     2461 aaggacgcgg gctggtgctg tcggttacgg gtaacaaggt gtccgccttc ggcacgcagt
     2521 cgcggttggc aagttggata cacggcgaca tcggcctacc gcctgtagac gaatctttac
     2581 gcaacgaggg gcacgggcag cctgtttcct tcgaatacat caaagagaaa aaccccgatt
     2641 ggattttcat catcgaccgt accgccgcca tcgggcagga agggccggcg gctgtcgaag
     2701 tattggataa cgcgctggta cgcggcacga acgcttggaa gcgcaagcaa atcatcgtca
     2761 tgcctgccgc gaactacatt gtcgcgggcg gctcgcggca gttgattcag gcggcggagc
     2821 agttgaagga ggcgtttgaa aaggcagaac ccgttgcggc ggggaaagag tagggagtcg
     2881 tctgaaaacg gagtttccga aggaagcggg gggtttctgc gaagctaaag tgcggtttca
     2941 acgaattgaa aagcagcctg tatgttgaaa ataccgctca agcaaacct
//
LOCUS       DAWHXZ010000209         2955 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000209 DAWHXZ010000000
VERSION     DAWHXZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.209"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..1221)
                     /gene="tyrS"
                     /locus_tag="WIE35_001829"
     CDS             complement(<1..1221)
                     /gene="tyrS"
                     /locus_tag="WIE35_001829"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ4154710.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLHQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEVLVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTD"
     gene            complement(1284..2942)
                     /locus_tag="WIE35_001830"
     CDS             complement(1284..2942)
                     /locus_tag="WIE35_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ4154711.1"
                     /translation="MRRIKRILPAFFAVLAATLAGGFFLFTKDDFFLLWKSALTALGF
                     ASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVARKSLRVQFGFLA
                     ALCALSLAASFIPSALDKYYLPHLRACELLIGSLTAVWMRCRQPAVGRRCAAVGALFA
                     VCILSTCLFSYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKKFFQSKITVAAGLI
                     SYSLYLWHWPILAFMRYIGPDNLPPYSPAAAVVLILLLSLFSYHCIEKPFKKWQGSFA
                     QSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNTGKQCLWGDTEKQ
                     PELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYASRVFQNWAACRAVY
                     RYAEEHLPRYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLHKLSSEKQAVYLMA
                     DNLASSYNVQRAYILSSRIPGYRQALRPDDESTLKANARIRELAAKYPNVYIIDAAAY
                     IPADFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH"
BASE COUNT          692 a          707 c          883 g          673 t
ORIGIN      
        1 gtcggtcagc aggcaggcat cgtcggggcg ttcggcggcg tggttggggt tgttggcttc
       61 ggcaggtttg ccgttgagca aaaccgcttt gctgttcaca aagccgcgcg cttctttatt
      121 ggaggatgcc aaaccggttt ttaccaagac ttcgacgaca ttgatgccgt ctgaaacttc
      181 aaatgcaggc agaccgtcga gggcgagctg ctcgaagtcg ctttcggtca ggctgctttg
      241 gtcttcggca aacaggcttt cggaaatgcg ttgcgcggcg gcaagggctt cttcgccgtg
      301 aatcaggcgg gtcatttctt cggcgaggat gcgttgcgct tcgggcttgc tgccgcttgc
      361 cttgtctttg gcttcgatgg catcgatttc ttcgatggac aggaaggtaa agtatttcag
      421 gaatttatac acatcggcat cggcgacttt cagccagaat tggtagaact gatagggcga
      481 ggtttttttc gcgttcagcc ataccgcgcc gccttcggtt ttgccgaatt tggtaccgtc
      541 tgatttggtt accaaaggca gggtcagacc gaatacttgt ttttggtgca ggcggcgggt
      601 caggtcgata ccggcggtga tattgcccca ttggtcggag ccgccgattt ccaaaaccgc
      661 gccgtggcgt ttgttcaact cggcgaagtc gtaaccttgc agcagggaat aggcgaactc
      721 ggtgaaggaa atgcctgcgc cgtcgcggtc gatgcgctgt ttgacggatt ctttgttcag
      781 catggcgttg acggagaaat gcttgccgat gtcgcgcagg aagtcaaggc agttcatgct
      841 gccgaaccag tcggcattgt tcgccataat ggcggcattt ccgccttcaa agctcaagaa
      901 aggggttaat tggttgcgga tactttccac ccagccggca acagtttcgg cggaattcaa
      961 gctgcgttcg gcggctttga agctggggtc gccgatcata ccggtcgcgc cgcccaccaa
     1021 agcaatcggc gtatgccccg cctgttggaa gcggcgcaat gccaatacgg gcagcaggtg
     1081 tccgatgtgc aggctgtcgg cggtcgggtc gaagccgcaa taaagggcaa ttttttgttc
     1141 gttcaacaaa gcgtctaagg cttcgatgtc ggtggtttgc gcgataaggc cgcgcgattg
     1201 caggtcttgg atgacgctca tcggtctctt tcaaaaaaaa ttagcgtttt tgcaaaccgc
     1261 cgattgtaac aaatttaagc gaatcaatgg ttatggcgcg tatcgagaaa ccgttgtttt
     1321 tcggaaaaac gctttgccaa ttccgtgccg ccgtaagggt tgatgtggtc tttgtccgag
     1381 taaaccggca atccgccgat ttgaaaatct gcggggatat aggcggcggc atcaataata
     1441 tagacgttgg ggtatttggc tgccaattcc ctgatgcgtg cattggcttt cagggtgctt
     1501 tcgtcgtccg ggcgcagggc ttggcggtaa cccggtatgc gtgaagacaa gatataggcg
     1561 cgctggacgt tgtaagacga ggcaaggttg tccgccatca ggtaaacggc ttgtttttcg
     1621 gacgagagtt tatgcagcat acggtcgaat ttttggaaaa aaccggcatc ataggcaagg
     1681 gagcggctgt tttcgggcat ttggctgccc cagcgcatcg ccaaaaccac ttttgaatac
     1741 cggggcaggt gttcttcggc atagcgataa acggcgcggc aggctgccca gttttggaac
     1801 acacgggacg cgtagccttc cacataggcg caagcgtcgg cggaaaccat agtggcggac
     1861 catttttctt ttttgcccac ggcatcgaag aatgttttgt aatggtcggc gtgggagtcg
     1921 cccaaaacca gcagttccgg ctgtttttcc gtatcccccc ataggcattg tttgccggta
     1981 ttgttgtggc aggaggtgtt ggaacgcgtc agccccaagc ggtcgtattg cgccataaac
     2041 ggcagtctca tcgcaaaaaa cgagcccgcc cccaaaatga gcataggcaa ggcataaatc
     2101 cataaaacgg attgtgcgaa cgaaccttgc cattttttaa acggtttttc gatgcagtgg
     2161 taagaaaaca gggaaagcag caatatcagg acgaccgccg ccgccggcga ataaggcggc
     2221 aggttgtccg ggccgatata gcgcataaag gccaatatcg gccaatgcca cagataaagc
     2281 gaataggaaa tcaaaccggc ggcaacagtg attttcgatt ggaaaaattt tttaagcggg
     2341 tgttcgtaat gattgaaata aatcagcgcg gcaacagcca gacagggaat caaagcggcg
     2401 gggcccggga aataggcggt ttgttccgaa taggaaaaca ggcaggttga caatatgcac
     2461 acggcaaaca atgcgccgac ggcggcacag cgtctgccga cggcaggttg ccggcagcgc
     2521 atccacacgg cggtcagcga tcctatcagt aattcgcagg cgcgcaggtg gggcaggtaa
     2581 tatttatcga gcgcggaagg tataaaggag gcggcaaggc ttaaggcaca cagtgcggca
     2641 aggaagccga actgtacgcg caggcttttg cgggcgacaa gcagcagcag tatcggaaag
     2701 acaaagtaaa attgttcttc gaccgacaaa gaccagatgt gcagcagggg cttttcttcc
     2761 tgcgcgggat cgaaataatc cttccccctt gcaaaataca ggttagaggc gaaacccaag
     2821 gcggtcagcg cggatttcca caaaagaaag aaatcatctt tggtgaataa aaagaagccg
     2881 cctgccagcg ttgccgccaa tacggcgaaa aatgcgggca gaatccgctt gatgcggcgg
     2941 atataaaatg ccttc
//
LOCUS       DAWHXZ010000210         2954 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000210 DAWHXZ010000000
VERSION     DAWHXZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.210"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..363
                     /gene="dut"
                     /locus_tag="WIE35_001831"
     CDS             <1..363
                     /gene="dut"
                     /locus_tag="WIE35_001831"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ4154712.1"
                     /translation="LRACLDEEVVLQPGETFLVPTGLAIYLANPSYAAVLLPRSGLGH
                     KHGIVLGNLVGLIDSDYQGELKVSLWNRSSEPFAVKPFERIAQMVIVPIVQARFKRVE
                     EFVGSSRGEGGFGSTGLH"
     gene            440..1627
                     /gene="dapC"
                     /locus_tag="WIE35_001832"
     CDS             440..1627
                     /gene="dapC"
                     /locus_tag="WIE35_001832"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ4154713.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            1790..2569
                     /gene="yaaA"
                     /locus_tag="WIE35_001833"
     CDS             1790..2569
                     /gene="yaaA"
                     /locus_tag="WIE35_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ4154714.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESEILMQQLRELALQ
                     QIAELMHVSDKIALLNAQRNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRAQLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            2652..2742
                     /locus_tag="WIE35_001834"
     tRNA            2652..2742
                     /locus_tag="WIE35_001834"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2686..2688,aa:Ser,seq:tga)
     gene            2820..2909
                     /locus_tag="WIE35_001835"
     tRNA            2820..2909
                     /locus_tag="WIE35_001835"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2854..2856,aa:Ser,seq:cga)
BASE COUNT          757 a          706 c          710 g          781 t
ORIGIN      
        1 ttgcgcgcct gtttggatga ggaagtcgtc ttgcagccgg gggaaacgtt tcttgtgccg
       61 acaggtttgg caatttattt ggccaatcct tcatatgccg ccgttttgct gccccgttcc
      121 ggcttggggc ataaacacgg cattgttttg ggcaatttgg tcggtttgat tgactccgat
      181 tatcaagggg aattgaaggt gtcgttatgg aacagaagca gcgaaccgtt tgccgtcaag
      241 ccgtttgagc gtatcgcgca gatggttatc gttccaatcg tgcaggcgcg tttcaaacgt
      301 gtcgaagaat ttgtcggaag cagccgtgga gaaggcggtt tcggcagtac gggtttgcat
      361 tgaatataaa atgccgtctg atgggtgccg tcaggttcag acggcatatc ttccgatata
      421 ccgacaaccg gagaacatta tgaatacctt actcaaccaa ctcaaaccct atccctttgc
      481 ccgactgcgt gaagcgatgc agggcatttc cgcccccgaa ggtctggaag ccgtcccact
      541 gcatatcggc gaaccgaaac atccaacacc gaaagtcatt acagatgcgc tgaccgcctc
      601 attgcacgag ttggaaaaat atccgctgac ggccggtttg cctgaactgc gtcaggcgtg
      661 tgcgaactgg ttaaaacgcc gttatgatgg gttgacgctg aattccgaca ccgaaattct
      721 gccggttttg ggcagtaggg aggcgttgtt ttcttttgtc caaaccgtgt tgaaccctgt
      781 ttcagacggc atcaaacccg taatcgtcag cccgaatccc ttttatcaga tttatgaagg
      841 tgcgacactt ttgggcggcg gtgaaatcca ttttgccaat tgccccgcgc cgtctttcaa
      901 ccccgattgg cgtagtattt ccgaagaggt ttggaaacgc accaaactgg tgttcgtctg
      961 ctcgcccaac aaccccagcg gcagcgtgct ggatttggat ggatggaaag aagtttttga
     1021 tttacaggat aaatatggtt tcattattgc ctcggatgaa tgctattccg aaatctattt
     1081 cgacggcaac aaacctttag gctgcctgca ggccgctgca cagttgggtc gaagcaggca
     1141 aaaactgctt atgttcacca gtttgtccaa gcgttccaac gttccgggcc tgcgttccgg
     1201 ttttgtcgcc ggcgatgccg aactgcttaa aaactttctg ctttacagaa cctatcacgg
     1261 cagtgcaatg agcattcccg tgcagcgcgc aagcattgcc gcttgggatg acgaacagca
     1321 cgttatcgac aaccgccgcc tgtatcagga aaaatttgag cgcgttattc ccattttgca
     1381 acaggtattt gatgttaaat taccagatgc ctcgttttac atttggttga aagtccccga
     1441 tggcgacgat ttggcatttg cacgcaattt atggcaaaaa gccgctatcc aagtattgcc
     1501 tggacgtttt ttggcacggg ataccgaaca gggcaatccc ggggaaggtt atgtgcgtat
     1561 tgctttggtt gccgatgtcg caacttgtgt caaagctgcg gaaaccattg tttccctata
     1621 tcggtaaaga ataaaaaata ccgtctgaac ttttgttcag acggcatttt tcaatatttt
     1681 acggttgaat ttgctataac ggtatttact tcacttttaa tctaatcggc ttgcccgcaa
     1741 acacgtttaa acttaaaatc cccgtgtttg acacaatacc gagcagatta tgttttttgt
     1801 cctttcccct gcgaaaaacc ttaacgaaaa agaccctgcc cctgtcagcg agtttaccca
     1861 acccgacctg ctggcagagt ccgaaattct aatgcagcag ctgcgcgagc ttgcgttgca
     1921 acagattgcc gaactgatgc acgtttccga caaaattgcc ctcttaaacg cgcagcgcaa
     1981 tgcagaatgg cacacgccgt ttacgccgga aaacgccaaa caggcggtct ttatgttcaa
     2041 cggcgatgtt tacgaaggta tggatgcaaa cacattggat attggacaga tacgctatct
     2101 gcaaaaccat gtccgcctgc tgtccggtct atacggtctt cttcgcccgt tagacctgat
     2161 acagccctat cgtttggaaa tggggacggc atttgccaat ttgcgcggca agaatttgta
     2221 tgagttttgg ggcgacatca ttaccaacct tttaaatgac acgcttgccc aagcaggcag
     2281 caatacgctt gtcaaccttg cctcacagga atatttcaag tccgtcaaca cgaaaacact
     2341 tcgggcgcag ctgattaccc cgatatttaa agacgaaaaa aacggtaaat ataaaatcat
     2401 cagtttctat gccaagcgtg cgcgcggatt aatggtgcgc tatgcggcag aacatcatat
     2461 taccgaccct gaaatgctga aaaattttaa ttacgaaggc tacgcattca atgacgcggc
     2521 ttcaaatgaa agcgaatggg tttttatgcg ttcggaacaa ataaagtgaa aacaataaat
     2581 taagtatttt ccgaaaaaag tgcttggcaa aatgtataaa cttcattatt attcttcttc
     2641 ttcaagaaga cggaagcgtg gcagagcggt ttaatgcaac ggtcttgaaa accgtcgagg
     2701 gttgatagcc ctccgtgagt tcgaatctca ccgcttccgc caatccttgg ggtcaaaaac
     2761 caaataaaaa tgaagtagca taaagcatcg gcatttttat ttggttttac ttcattatcg
     2821 gaaacgtggc agagaggctg aatgcagcgg actcgaaatc cgctgagggt gcaaatcctc
     2881 cgtgggttcg aatcccaccg tttccgccac aaaacaaaac cgccctgatt cggggcggtt
     2941 cttttttgtt caag
//
LOCUS       DAWHXZ010000211         2921 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000211 DAWHXZ010000000
VERSION     DAWHXZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.211"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(51..227)
                     /locus_tag="WIE35_001836"
     CDS             complement(51..227)
                     /locus_tag="WIE35_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154715.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(230..1588)
                     /locus_tag="WIE35_001837"
     CDS             complement(230..1588)
                     /locus_tag="WIE35_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ4154716.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMMAKAWRENVDPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1713..2876)
                     /locus_tag="WIE35_001838"
     CDS             complement(1713..2876)
                     /locus_tag="WIE35_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ4154717.1"
                     /translation="MTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQY
                     VAKRAHILEAAKKASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQT
                     KGLLINGYQSTAHATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFAN
                     EISTYALRSDYPWFTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSH
                     SDFCTRLDKDARRFQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHG
                     LMHVGKGGERTLTHGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGI
                     ETDELPDDGYDFWGNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
BASE COUNT          696 a          831 c          671 g          723 t
ORIGIN      
        1 cgtttgaggt tggcttgccg cccggcaagg tttcggacgg cttttgcaga ttaacgtgca
       61 gggactttct caatcacgca aattgggtaa ctccccggat ttttaccgcc gcccaagtct
      121 aaacataaat cttcataaag atacgcctgc gtccgcattc cggcataaaa cgcgccgacc
      181 agcgcggcaa tcagcaataa ggctttaaaa aaacgtcggc ttttcatttt cacttatcct
      241 ccaatgcctc gaccacttct tccatctgca taaagcgcga acctttcacc aacacggtgg
      301 cgcgttcggg caaatcatgg cgcaacactt gaatcaacgg gtctttggcg gcgaaccaca
      361 aaccgtccgc gccaaatttt tccgccgctt cgacgctgtt gtcgccgaca aaataagccg
      421 cttcgatgcc ttggtcgcgg gcatacgcgc cgacttcggc gtgcatagcg gcggcttcgt
      481 cctcgcccag ttcgcccata tcgcccatta cgaaaatacg cggcgcaggc atacgcgcca
      541 acacgtcaat cgcggctttc atgctgtccg ggttcgcatt ataagtatcg tcaatcaggg
      601 ttgcgccctt gattccggat ttgacgttca gacggccttt gatattgctg aagcctttca
      661 aaccttccgc cacatcgttc aaactcaaac ctgcagccaa agacagcgcg gcggcagcgg
      721 cggcgttgtg gacattgtgg cggccgggaa caggcagcac cacggcggcg cgttcgtcgc
      781 cgcacaccaa atcaaattcg cacgacaacg gtttcagcac gatattttcc gcgtggacat
      841 cgccgctatc gatgccgaaa gtgcgcgtat tcaaattaag cgttgccgtt ttgaagacag
      901 ccatattggc atcttcttga ggaatcagtg ccatgccgtc tgaacataag ccttgataaa
      961 tctcgctttt cgctttggca atatcgccca ctccgtcgaa accgcagccg acatgggcgc
     1021 gcatggcgtt gttgaccaat gcggcatcgg gtttggcgat ttgtgtcaaa accgccagtt
     1081 cgccaaaatg gttcataccc atttcaatca cggcatagcg gtgtttttcg tttaatttca
     1141 acaaagtcaa cggcaatccg atgtggttgt tgaagttgcc tgccgtcgcc aaaacggcat
     1201 catcaccgaa acggcggcgc aataccgcag ccagcatttc cttcaccgtc gtcttgccgc
     1261 ccgaaccggt aatgccgaac acaaacggat ccacgttttc acgccacgcc ttcgccatca
     1321 tttgcaacgc ggtaagcgtg tcatcgactt tcaacgcgcc atccattgca acgcaatctt
     1381 cgcgcgaaac cacaaccgcc gccgcaccag cagccaatac gtcttcaaca aaatcatgcg
     1441 catcaaaccg cccgcccgcc aatgcgaaaa acacatcgcc cgcgcggatg tcgcggctgt
     1501 cggttacgat gcgcgacacg ggtttgcttt cagacggcat cggaagcttg agggcttggc
     1561 agatgaaatt taggtccagt ggtttcatat ttactttcgt taatattcgg gcggcggaca
     1621 catcggtagc ggctgatttt tttatcgcct gttttgctgt ggtaaaacac agattatttt
     1681 cccattctca ttcggcattt tttctgtacg tatcattttt tagacgtatt tttagtcgat
     1741 ttgccttttc ccgcatacca cggcgcgggg tcgtcgggca gtccgtcgat aaaggcaagg
     1801 ttattgcctt cgccctgcac atcgggaaca ttcccccaaa aatcatagcc gtcatcgggc
     1861 aactcgtcgg tttcgatacc cgtccaactg ccgaatccgc gtaaaaaatt aaacgcgctg
     1921 cgcctcactt taatcatttc gcgccgcgtg tcatcggacg aaattttaac cagcggcacg
     1981 ccgtagcttt gacgcttcca cgcaccatgt gtcaacgttc gctcgccgcc tttaccgaca
     2041 tgcatcaaac cgtggtcgga aaagtaaacc aaagaccagc tttctttatt ttcattcaat
     2101 atcttaactg tatcttctaa aaatttatcg gtttgcgcga tggtggaaac atagcaggat
     2161 attttttcag tttgatactg aaaccgccgc gcatccttat ccaaacgtgt gcaaaaatca
     2221 ctgtgcgaac ccatcaggtg catcacaatc agccgaggct tcgtgcctgc atttcctatc
     2281 aaaacccgtt tgaacgccgg caacaaaagg cggtcgctca accccgcgct tttgccataa
     2341 tcaccccttt gggtaaacca cggataatcg ctgcgtaggg cataggtgga aatttcgttg
     2401 gcaaaatgcc ccaacattcc ttgattagac agccacgccg tccgaaaacc cgcctgcttc
     2461 gccaagctga cgatgttatt gttcggttct cccggcagcc ccaaagtctg cggcagcgaa
     2521 agattcgtcg cgtgggcggt cgattggtaa ccgtttatca acagcccttt ggtctgactc
     2581 aaaaaaggcg tatcgggcaa tgggaaaccg taaacattca tataatccga acgcgcgctc
     2641 tcaccgataa ccacaacata atttttatac ttgggcgcaa catgacggat atgccatgtt
     2701 gacgcttttt ttgctgcttc caaaatgtgg gcgcgcttgg cgacatattg tgccggcgcg
     2761 gaagccaaat cgtaatacag gtcgaaaata ttcaacaaca ggccggcatc aggttctcgc
     2821 aaatctttat cgctggcgat tttatccatc accgcgcagg acaaaatcaa agtcaataat
     2881 atagtcagcc atattttgct gcggcgttta tagtttttta c
//
LOCUS       DAWHXZ010000212         2907 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000212 DAWHXZ010000000
VERSION     DAWHXZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.212"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            78..>2907
                     /gene="recB"
                     /locus_tag="WIE35_001839"
     CDS             78..>2907
                     /gene="recB"
                     /locus_tag="WIE35_001839"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ4154718.1"
                     /translation="MSAPIQAFDSLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDGALSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLIPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAECRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPHRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVTDNAPSGTNFAFTEDAPPPAAYRGNAGQTAE
                     FAANSIPERRFRFVRHTSFTALSRHTQTP"
BASE COUNT          703 a          967 c          718 g          519 t
ORIGIN      
        1 actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatac cgtccaaccg
       61 ttttccgagc gcgcactatg tctgcaccca tccaagcatt cgactcgcta accgtcccca
      121 ttgccggcac caacctgatt gaggcctctg ccggcaccgg caaaacctac ggcattgccg
      181 ccctgtttac gcgcctgatc gtattagaac aaaaaaacgt cgagcgcgta ttggtcgtta
      241 ccttcaccaa agccgccacc gccgagttga aaacacgcct gcgcgcacgt ttggacgatg
      301 tgttgcaggt tttagaaagc aaagaaattg ccgaacttgg agacggcgcg ctttcagacg
      361 gcatcgccgc ctattgtgcg gaacaccaca aaggcgacac cttcctgccc gcactcttaa
      421 aacaggcttt gcaaaaagag ggccggacgc gtctgattgt ccgcctcaag gccgccatcg
      481 ggcaattcga caacgccgcc atctacacca tccacggctt ctgccagcgc atcctgcgcg
      541 actacgcctt cttgtgccaa gcaccgttcg atgtcgaact gaccgaagaa gacggcgacc
      601 gcctgcttat cccggcgcaa gatttttggc gggaacgcgt cagcaatgat ccggtgcttg
      661 ccgcattggc gtttaaacgc aaagctgtgc cgcaaaccgt ccttgcccaa atccgcgcct
      721 acctgtcccg cccgtacctg aatttccgcc gtccgcaggc ggatttgaaa caggcgcagc
      781 gcaacgccga aacctcatgg caaaccgtct gccgcctgct gcccgagctg gaagccggct
      841 tttggcgcat ccatcccgac ctcaacggca acagttaccg caaaaacagc ttcggcaacc
      901 tttttaaaga actggcacaa aaatccgccg ccggacaact gccctttctg gacaaagaca
      961 cacacgacag actcttgaaa cttgcatccg acaaactcga agccggactg aaaaaaggca
     1021 aaacgcccga tgcggcagta tttgccgaat tgcagaaact ggcagacttc gggcgcgatt
     1081 tgaacgcact cgaagaagcg gaagaagcaa caatgatccg gctgcaactg gatttaatcg
     1141 aatacctcaa ccgcagcctt gccgagatga aaaaatcgcg ccgcgaacgc ggtttcgacg
     1201 acctgctgct cgatgtccac accgcgctga ccgacaatcc gcacgccgaa accctcgccc
     1261 gcgccgttgc cgaaaactgg gaaaccgcgc tgatcgacga gttccaagac accgacccgc
     1321 tgcaatacga aatcttccaa aaaatcttca tcgcccaaaa ccgaccgctg ttcctcgtcg
     1381 gcgatcccaa acaggcgatt tacagctttc gcggagcgga catttacgcc taccttcagg
     1441 cggcggaaga cgcgcggcac cgctacacgc tcgccaccaa ctaccgcagc cacgccgcgc
     1501 ttatcggcag cataggcgcg ctgttccgcc tcaaagaacg cccgttcgtt ttggaaaaca
     1561 tcggctattc ggaagtcggt gcggcgcgtg ccgaaagcag attgtcgccc aaacgtcctg
     1621 ccgtacaggt tcgttggctg cacgaaaacg acaatgaaaa agccaacaaa gacgttttgc
     1681 gccgccgtgc cgccgactat tgcgccgacg aaatcgccca cgcgctcaac gaagccgccg
     1741 aatgccgtct gaatttcaaa ggctgcccgt tgcagtcggg cgatattgcc gtgctggtac
     1801 gcacgcacaa cgaggcggtg atggtttccg ccgccctgaa aaaacggcag gtgcaaagcg
     1861 tcctgctttc gcgcgaatcc gtgttcgcct cgcccgaagc cgccgccctg tccgcactca
     1921 tcggcttctg gctcgaaccg caccgcgccg gaacgctgcg ctttgtccta acaagcggca
     1981 tattcggtta tgacgcgcag caattgcacg acttcaacca aaacgaaagc gagattttgc
     2041 attgggcgga atctgcccga accgcgctcg acaactggaa tcaatacggc attttcgccg
     2101 ccatgcagca attttcccaa acacacggca tcgaaacacg cctcttaagc cggaacaacg
     2161 ggcgcagcct gaccaactat ttccaactgc tcgaactgct tgccgccgaa gacgcgcaaa
     2221 accgcaaccc cgccgcgctg cacaaatggc tgcgcgacca aatcagcctg gccggcaaca
     2281 acggcggcga caaccgcgcc atccgtctgg aaagcgacga agatttggtc aaaatcgtta
     2341 ccatgcacgc ctcgaaaggt ttgcagtatc cgctggtgta ctgcccgttt gcgtgggacg
     2401 cgcaagatac cgggccgtcc gactggcaaa tcctccacca aagcgcaaac cgaaccgaac
     2461 tgttggcaaa ggcgcaactg tccgaagacg aacagaaaca atacgccgat gaagaaatgg
     2521 cggaacgcct gcgcctgctt tatgtcgcgc tgacgcgtgc cgaggaacag ctcaacatct
     2581 acgccgcgta ctccaccaat actgccgaca accccctcgc ctacctgatt gaaggctcgc
     2641 cccaagacag ccgtgaaacc gtccgccgtg cctatgcgtg tgaaaaagac ggcatcgcga
     2701 tgctcaaacg caactggcgg cgcgtgacgg acaacgcccc ttccggcaca aatttcgcct
     2761 tcacagaaga tgcgccgccc cctgccgcct atcgcggcaa cgccggtcaa accgccgaat
     2821 ttgccgcaaa cagcattccc gaacgcagat tccgatttgt ccgccacacc agctttaccg
     2881 ccttaagccg ccatacccaa acgcccg
//
LOCUS       DAWHXZ010000213         2904 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000213 DAWHXZ010000000
VERSION     DAWHXZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.213"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(389..1189)
                     /locus_tag="WIE35_001840"
     CDS             complement(389..1189)
                     /locus_tag="WIE35_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ4154719.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTMAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(1325..1828)
                     /locus_tag="WIE35_001841"
     CDS             complement(1325..1828)
                     /locus_tag="WIE35_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154720.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(2165..2806)
                     /locus_tag="WIE35_001842"
     CDS             complement(2165..2806)
                     /locus_tag="WIE35_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ4154721.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMTMSEKSGVPLYFDRAL"
BASE COUNT          930 a          622 c          504 g          848 t
ORIGIN      
        1 ctcttttggg cttattcaaa ttagggacac gccctagaac caagttgcat gataatacct
       61 ttcattaact gaaacactga ttaagaaact ccagtctgtc taatgatgag gttttcacat
      121 cgccaaaact tgccaatcaa atgctggatt tattgccgtc tgagatttgg tcaaatccaa
      181 aggcgacatt cttagaccct gtgtgtaaat caggggtatt tttgcgtgaa atcgtcaaac
      241 gcttggatga aggcttgacc aatcaaatac cagataaaca aactcgcatt aaccacattt
      301 taaaaaatca agtttttgga agtactgcca cgtatgtagg tagctttgac cgatatttgc
      361 ataaaaactc ctttgctggt gaaaggaatt attttgccaa ttttaaaata tttctggcac
      421 caaatagtac aatgacaaag acaatcatgc caatgattaa atcaggatag ctagaatgag
      481 tcaataacgt caatgctccc gccgctatca caccgatatt gatgataatg tcattggatg
      541 taaaaatcat gctggctttg atatggattt ctttattttg atttttgctc agtagatata
      601 agcacagcca gtttgcaatc aatgccaaaa atgccgtgcc aatcatcagt tgataattgg
      661 gcagctgctc agcaccgata aaacgcctaa tcacttctat caccccaaat aacgccaata
      721 ttatctgcgt tatccccgcc aaaaatgcca cacgtttttt atacgccatc gtcataccaa
      781 tggctgatag cgccaatata tagacaaagc tgtccgccag catatctaga ctatcagcaa
      841 tcagccccat agaattagca aaaataccaa ccgaacactc tatgataaaa aacacaaagt
      901 taatcatgag cacttgatat aataatcttt tttctaagtg ctcatcaggc ttgttaaaca
      961 ctatcttatc aacaatcact tcggtggaaa tgatatgact atcaaaatta agcggttcaa
     1021 gtacttgtaa aatcgttgta tcttgattat cgtgatagac ggttaagcac cgcccagcaa
     1081 tatcaaactg taattcataa atatcagaca catcttttaa acgcatgcga atgagctgtt
     1141 cttcggacgg gcagtccatt ttggtaatgt taaaaatggt ctttttcatc tatttagttc
     1201 cttgttttga tcaggttggc tcaaataaat ctgtgtttat attgctgctt ggtaattttt
     1261 ggatggtttg agtaaattga ttaggttaaa atttaccttt ggaagtaccg ccacgcataa
     1321 tagtttagat atgtttataa tctctggata aaaaaacgta ataagtgctt actggataac
     1381 aaagtccaaa ccaatagcag gcaaaataag gcatccaccc cccttcttca ttaaggatat
     1441 atattgagaa acaaatcgca actaaacaga aaaaacttgg gagataaagc catttcattc
     1501 ccctattcaa gaatctagcc aagataggta ttttgtattc tacaaaaaag aaaggcattt
     1561 ccaagggaaa catgtcagat aaaaactttt gtttattttt tactatagat agaaccttgc
     1621 ttctcaagag aaagccatta ataataccga tgacagctat taatatatag agaatagtat
     1681 aagtatgaat aatcttcatt agacaaaaag aagaaatggc agataaatta catacgatat
     1741 attggaatat aaaatattta cggtctaaac cttgttcagt tgcaattttt ttaaaattgc
     1801 cttgcataaa aaaatcaaag gcgtccatta aactatcttt cacattagaa atttaaaagc
     1861 taaataatac gacaaacaat gtgaagtact attcatggtt tatttaaaaa ataatactat
     1921 tctgaacatt atttagatac agaaattaac aaattagaac taaacaagct tttaaatact
     1981 ttaattttat tggaaagcta taaaaggaac tataacttta cacactagtc acttcttttt
     2041 aagaggcaaa agggattggg aaggtcgtct tggagataag cactggtatt tcggccaatg
     2101 gtaaatagag tttacctcaa atagggtaga acctccttca tctgtcagtt aataacagcc
     2161 acttttacaa tgccctgtca aaataaagcg gcacgcccga tttttcactc atcgtcatca
     2221 aataacccat caccttttgg ggccattcga tgccgcgcac cacggtcaga ttcctcaaaa
     2281 cggggaaaac caaaatatcc tccataccga ttccgccgtt gatgccgtct gaagcaccgt
     2341 ccatcaaatt ttccaactct tgcaaatctg cgtttatgcg ttcgagatat tgggcggttt
     2401 tattcaaatt ggcggaaaag ctgccgatgc ttttctcttt tttgtctgta aaatatttta
     2461 ccgcttccgg cgttgcaaat tcgggcagcc cgattttgat cacgcgcggc tgcaccagtt
     2521 tgtcgttgta tccgcccacc ttgtccagcc acgcccgtat ctcggggcgg acttcgtctt
     2581 tcagacggtc ttcgcggtcg aaatgccgca caatgtccaa actctcgccc ataaacgaac
     2641 cgtcttcttt ttgcaggacg ggcacttgtt tcgcaccgat cataccgatc ggcgttgcct
     2701 cgtcgtcgtt tgccagcacg gcttcttcaa cgtccgcgcc aaacagcccg gcagccatcc
     2761 gcgcacgcac acaaaacggg caatggtcgt aaatatacag tttcatcaaa atattcctcg
     2821 tcaacctgtc ggtaccggct accttaacac cccgcgccac ccgaaacaag tttatcttcc
     2881 cgcctatgca ccgtaaataa ataa
//
LOCUS       DAWHXZ010000214         2844 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000214 DAWHXZ010000000
VERSION     DAWHXZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.214"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(53..529)
                     /gene="greA"
                     /locus_tag="WIE35_001843"
     CDS             complement(53..529)
                     /gene="greA"
                     /locus_tag="WIE35_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ4154722.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            921..2222
                     /gene="aroA"
                     /locus_tag="WIE35_001844"
     CDS             921..2222
                     /gene="aroA"
                     /locus_tag="WIE35_001844"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ4154723.1"
                     /translation="MTESLRLPAASLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYRLHGVARMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIATTPVRVTGIGANSIQGDVA
                     FACELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAVVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVAEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLVSLLDVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
     gene            complement(2265..2798)
                     /locus_tag="WIE35_001845"
     CDS             complement(2265..2798)
                     /locus_tag="WIE35_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ4154724.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGTHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          601 a          783 c          766 g          694 t
ORIGIN      
        1 ttaatccact ataatttcct gcgtgtgtcg ggtgtatcga aatcaagccg aatcaaatat
       61 atcggacttc gataatgtcg tattcgcgca cgccgcccgg ggcttggact tccgccgtat
      121 cgccctcttc cttgccgatt aaggcgcggg caatcggtga gccgacatag attttgccct
      181 gtttgatatc cgcttcgtct tcgccgacaa tttggtaggt aacgtgttct tccgtttcca
      241 aatcttccag cgtaaccgtc gtaccgaaca cgattttgcc ttcggcgtgg atttcggtcg
      301 gattgatgat gtgggcaacg gaaagtttgt gttccagctc ggaaatgcgg ccttcgataa
      361 agccttggcg ttctttggcg gcttcatatt cggcgttttc ggacaaatcg ccgtgcgatc
      421 tggcttcagc aatcgcttcg atcacttcgg ggcgcgccac gcttttaagc tgctgcaatt
      481 cctgtttcag caattccgca ccgcgtacgg tcagggggat tttttgcatc ggtctttttt
      541 ctccatattc cggcacaccg gtttgcggca gcaaacatac cgcataccgt cttgttttgt
      601 gcgtccggat attaaaataa aaacacaagc cgcccggaaa atcggcggct tgtctgtcgt
      661 tgaacagcgg ctattctacc aaattctatg aaattggcaa tcgtgccgtg ccgtccgcaa
      721 acacgctatg tccgcaacaa aagccgaaaa tatgccggca aaaaaatttt agaaacaaaa
      781 aatttaaaaa taatcaattt tcggcacaaa aaaccatatt cacggacttt aaaaccgaaa
      841 atgccaaacc tgagattttt cagacggcat ttgcaccagt ataatgcagg ctgtttttat
      901 ctttatattt acgttttgcc atgaccgaat ccctccgcct ccctgccgcc tcgctcaaac
      961 cttccactgt cgccctgccc ggctccaaaa gcatcagcaa ccgcaccctg ctgcttgccg
     1021 ccttgtccga caatgcttgc gaaatccatt ccctgctcaa atccgacgat accgaccgta
     1081 tgctcgaagc actcgataaa ctcggcgttg aaatcgaata tcttgccgaa gaccgtctga
     1141 aagtgcacgg cacaggcgga cgcttcccca accgcactgc cgatttgttt ttgggcaacg
     1201 cgggcacggc gttccgcccg ctgaccgccg cgcttgccgt tttgggcggc gattatcgcc
     1261 tgcacggcgt tgcgcgtatg cacgaacgcc ccatcggcga tttggccgat gcactgcgga
     1321 ttgccggcgc cgatgtcgaa tatctcggca aggaacacta tccgccgctt catatcggcg
     1381 aacgccaaga caacggcgag cgcgtgattc cgattaaagg caatgtatcc agccagtttc
     1441 tgaccgccct tttaatggcg ttgccgctga ccgggcaggc gtttgaaatc cgtatggtcg
     1501 gcgagttaat ttccaagccc tatatcgaca ttactttaaa actgatggcg caattcggcg
     1561 tacaggttat caatgaaggc taccgcgtct tcaaaattcc cgccgatgcg cactatcacg
     1621 cgcccgaaca cttgcacgtc gaaggcgatg cctccagcgc gtcctacttc ctcgcagccg
     1681 gtttgattgc caccacgccc gtccgcgtta ccggtatcgg cgcaaacagc atacagggcg
     1741 atgtcgcctt tgcctgcgag ctggaaaaaa tcggtgcgga cgtggtttgg ggcgaaaact
     1801 tcgtcgaagt ctcgcgcccg aaagaacgtg ccgtccaagc ctttgatttg gatgcaaacc
     1861 atatccccga tgccgccatg accctcgccg tcgtcgcgct tgctacaggg caaacctgca
     1921 cgcttcgcaa catcggttcg tggcgcgtca aagagaccga ccgcattgcc gcaatggcga
     1981 acgagttgcg caaactcggt gcaaaagtcg ccgaagaagc cgaagcaatt cacatcaccc
     2041 cgcccgaaac gccgacaccc gacgccgtca tcgacactta cgacgaccac cgcatggcaa
     2101 tgtgtttctc gctggtttcg ctgttggacg tacccgtcgt catcaacgat ccgaaatgca
     2161 cccacaaaac cttcccgact tattttgaag tgttctcatc gctgaccgaa acagcggaat
     2221 aaggcggcat tttgccgtga ttccggcgca gcgcggcggg cggctcattc tgtaaaaaaa
     2281 gtatgtgcgc cgaggtagtt tttggcgtaa aacggtgtgg agagtttttc ggttttgatg
     2341 gttttgccgc tgctgggggc atggatgaat tcgccgttgc cgatatagag tccgacgtgt
     2401 gagtagcggt gtgtgccgcc ggtgttgaag aatacgaggt cgccggcctt aaggcggctg
     2461 tcggggattt tgcggcttgc cgccgccatg tcgcgggcgg tgcgcggcag cttgacgttg
     2521 agggcgtttt tgtaaacgaa ttgaatcatg ccgctgcaat cgaagccggt tgcggtgctg
     2581 ctgccgcccc atttgtaggg cgtgccgatg agtccgaggc tgtggagcat gagttcctgc
     2641 gagccttgtg tgcggtcgat gtggctgatg cggacggctt ggatttgccg gactgtctgt
     2701 ttgggtttcg gttggcggtg tttgccggag gtcgtgccgc atgaggcgag gagcagtgcg
     2761 ctgagacaga ggaaaagggt tttgtcgggg ggaaacatgg tttttccttt gcgggttcgg
     2821 atatccgtct gaaggcgttt caga
//
LOCUS       DAWHXZ010000215         2783 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000215 DAWHXZ010000000
VERSION     DAWHXZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.215"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(52..1221)
                     /locus_tag="WIE35_001846"
     CDS             complement(52..1221)
                     /locus_tag="WIE35_001846"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ4154725.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGLL
                     PWLRPDAKAQLTVVYDSESGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1404..1514)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1567..2463
                     /locus_tag="WIE35_001847"
     CDS             1567..2463
                     /locus_tag="WIE35_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ4154726.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2507..>2783)
                     /locus_tag="WIE35_001848"
     CDS             complement(2507..>2783)
                     /locus_tag="WIE35_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ4154727.1"
                     /translation="QTGFRTVVVAGGVGANRKLRETFGSMTVQIPTPTGKPKHPSEKV
                     SVFFPPTAYCTDNGAMIAFAGAMHLGKGREVGAFNVRPRWPLSEIVK"
BASE COUNT          627 a          752 c          750 g          654 t
ORIGIN      
        1 aaaaatgccg tctgaactgt tcagacggca tttttgattt tcaaaccgga attacagccc
       61 cgctgccgcc ctcaatgcag cagctttgtc ggtgcgctcc caagtgaact caggttcttc
      121 gcggccgaaa tgtccgtaag cggcggattt actgtaaatc gggcgcaaga gatcgagcat
      181 ttggacgatg cctttggggc gcaggtcgaa atgttcgcga actaaggtaa tcagtttttc
      241 ctcgctgatt ttgcctgtgc cgaaagtgtc gatggaaatc gaagtcggtt cggcaacgcc
      301 gatggcgtag gaaacttgga tttggcattg ggttgccaaa ccggcggcga cgatgttttt
      361 cgcaacgtag cggcaggcgt aagcggcgga acgatccact ttggacgggt ctttgccgga
      421 gaacgcaccg ccgccgtgcg gagccgcgcc gccgtaggta tcaacgatga ttttacggcc
      481 ggtcaaaccg cagtcgcctt gcgggccgcc gatgacgaag cggccggtcg ggttgatcag
      541 gtatttggtt tcgtcggtca gcagttcaga cggcaggacg ggcttgataa tctgctcaat
      601 gacggcgttt ttcaactctt cataaccgat ttccgggtcg tgttgcgtag agaggacgac
      661 ggtatcaatg cgttttactt tgccggattc gctatcgtaa accacggtca gttgggcttt
      721 tgcgtcggga cgcagccaag gcaggaggcc gtctttgcgt aattcgcttt gacgctgcat
      781 caggcggtgg ctgtaataga tggcaaacgg catcagggtc ggggtttcgt cgcaggcata
      841 gccgaacatc aaaccttggt cgccagcgcc ttggttcaag tcaatgcctt cgccttcgtt
      901 cacgccttgg gcgatgtccg gagattgctg gtcgtagtac acgccgaccg cgcagccgtt
      961 ggcatcaaag cccagctcgg aggagttgta gccgatgcgt ttgatggttt cgcgtgcgac
     1021 tttgatgtag tccacttggg cggtggtggt gatttcgcct gccaatacgc acaagcctgt
     1081 gttgaccaaa gtttctgcgg cgacacgcgc tttggggtct tgcgccaaga tggcatccaa
     1141 aatcgcgtcg gatacttggt cggcaacttt gtccggatgg ccttcggata ccgattcgga
     1201 agtaaacaga tattcgctca ttgagatttt ccttgaaaaa caaaccatct tcttcagacg
     1261 gcatgttgta tgaacataat gtcgacagcg ggaaatatag caaaatttcc ctattcatac
     1321 cattcagttg agaaatattc ccatttgaat ggcactttgg aatctctgcc cgtacgtttc
     1381 ttacaggcaa aaaaattccc gcatcaagcg ggtttggatt gctctggtga gccacatcgg
     1441 cttttcaacc gtccacctta cttttccttt tgaaaagcag gttggcatgg aattcccaac
     1501 tcttaatgca gcgcggatgg tagcagaaaa gcctttccgc cgcaatactt ccctttttca
     1561 gacggcatgt ttcgtttaca attcgggctg tttccccctt tgcgaaccgc catgcacatc
     1621 ctgttgaccg ccctgctcaa atgcctctcc ctgctgccac tttcctgtct gcacacgctg
     1681 ggaaaccggc tcggacatct ggcgttttac cttttaaagg aagaccgcgc gcgcatcgtc
     1741 gccaatatgc gtcaggcagg catgaatccc gaccccaaaa cagtcaaagc cgtttttgcg
     1801 gaaacggcaa aaggcggttt ggaacttgcc cccgcgtttt tcagaaaacc ggaagacata
     1861 gaaacaatgt tcaaagcggt acacggctgg gaacatgtgc agcaggcttt ggacaaacac
     1921 gaagggctgc tattcatcac gccgcacatc ggcagctacg atttgggcgg acgctacatc
     1981 agccagcagc ttccgttccc gctgaccgcc atgtacaaac cgccgaaaat caaagcgata
     2041 gacaaaatca tgcaggcggg cagggttcgc ggcaaaggaa aaaccgcgcc taccagcata
     2101 caaggggtca aacaaatcat caaagccctg cgttcgggcg aagcaaccat cgtcctgccc
     2161 gaccacgtcc cctcccctca agaaggcggg gaaggcgtat gggtggattt cttcggcaaa
     2221 cctgcctata ccatgacgct ggcggcaaaa ttggcacacg tcaaaggcgt gaaaaccctg
     2281 tttttctgct gcgaacgcct gcctggcgga caaggtttcg atttgcacat ccgccccgtc
     2341 caaggggaat tgaacggcga caaagcccat gatgccgccg tgttcaaccg caatgccgaa
     2401 tattggatac gccgttttcc gacgcagtat ctgtttatgt acaaccgcta caaaatgccg
     2461 taacgaaaat aaaaatgccg tctgaacaat ttcagacggc attttgtcat ttgacgattt
     2521 ccgacagcgg ccagcgcgga cggacattga acgcaccgac ctccctgccc ttgcccaggt
     2581 gcatcgcacc ggcaaaggca atcatggcac cgttgtccgt gcagtatgcc gtcggcggga
     2641 aaaacacgct gactttttcg gacggatgtt tcggcttgcc tgttggggtc gggatttgca
     2701 ccgtcatgct gccgaaagtt tcacggagtt tgcggtttgc accgaccccg ccggcgacca
     2761 ctacggttct gaaccctgtc tgc
//
LOCUS       DAWHXZ010000216         2778 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000216 DAWHXZ010000000
VERSION     DAWHXZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.216"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..180
                     /locus_tag="WIE35_001849"
     CDS             <1..180
                     /locus_tag="WIE35_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154728.1"
                     /translation="RRLVLIFVNPLYNVGIGSGALLGHWVTQYVGISRIGIAGMLAAA
                     AGLWLCLNLNRHIRT"
     gene            308..1744
                     /locus_tag="WIE35_001850"
     CDS             308..1744
                     /locus_tag="WIE35_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ4154729.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFSWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RALNIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRDALCRLKYLAPLSWFVTFHFVCLSFVVFNTANPD
                     DAGAVFSALFANASGWNAPQQADMLLLASFASVMLLYPYLQRAFDGVVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            1755..2738
                     /locus_tag="WIE35_001851"
     CDS             1755..2738
                     /locus_tag="WIE35_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ4154730.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGVALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGET
                     EWKQDTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
BASE COUNT          563 a          874 c          691 g          650 t
ORIGIN      
        1 cgtcgccttg tcctgatttt tgttaatcca ctatacaacg tcggtatcgg cagcggcgca
       61 ctgttgggac attgggtaac gcaatatgtc ggtatttccc gtatcggcat cgccggtatg
      121 cttgcggcgg cggccgggct gtggctgtgc ctgaacttga accgccatat ccgaacctga
      181 cccgcgatgc cgtctgaagc ctctcccgcc cttcagacgg cattttgatt gaagaaatat
      241 ccacccctgc ttaaaataac gggctttgcc gtgttttaac gcctattttt tgttccaagg
      301 atggtttatg ccgctgctgt ctgtcgagtt cgcactgttc tttctcgcct tcctgccgat
      361 ttactggggc ttggcgaaat acccgtccgt ccaaaacctg ctgcttttgg ctgccggtat
      421 gggctggctc taccatatcg gccccgtatt tgcggcaatc gtcgtccttt attcctcctg
      481 cgtgtacctt ttgggcgaac tgctccgttc cgatcgcgaa aatacgcgcc gcttttggct
      541 ggggtgcggc attgccgtct cgctgaccgt cttgggcttt ttcaaatatt tcgacttttt
      601 ccgcccgctg attgcccaat atgccggaaa aggcggcgca atcgacatcc tgatgccgct
      661 ggggctttcg tattacacct tccagtcgct cgcctatctg gtttactgct tccgcgcccc
      721 gcacgctgcg cgtttcagct ggcacgagct gctactacac ctgagtttct ttcccaccgt
      781 tacctctggc ccgattatcc gcgccgccgc attcaaaagc gcggacggcg agcaggcggg
      841 cgcattggcg caaatccgta cccgccaacc gcgctcaccc gtccgccccg cactcgccgt
      901 ttccctgatt ctgctgggta ttgccaaaaa atggtggctg gcggggatgc tggcggaaaa
      961 ctgggtgtcg cccgtatttg aaaatcccgc ccaattcgac ggctggggcg tattgggcgg
     1021 cgtgtacggc tataccttcc aactcttttt agacttttcc ggatattccg atttggttat
     1081 cggcatggcg atgctgctgg gtttccggct gcccaaaaac ttctccgcac cgcttcgtgc
     1141 tttaaacatc cgcgcatttt gggacaaatg gcacatcagc ctttccacct ggatacgcga
     1201 ctacatctac atccccttgg gcggcagcaa aaaaggcttt ttacggacac agctcaacct
     1261 gatggcggca atggtactct ccggcatatg gcacggctac ggctggaact tcctcatttg
     1321 gggcgcgctg cacggcacgg cgctggtgct gctcaacacg ggcgaccgtt atttcggacg
     1381 cgacgcgcta tgccgtctga aataccttgc gccgctctcg tggtttgtta ccttccattt
     1441 tgtctgcctt agctttgtcg tcttcaatac cgcaaacccc gacgatgccg gcgcggtttt
     1501 cagcgcgctc tttgccaatg ccagcggctg gaatgcgccg caacaggcag acatgctgct
     1561 gcttgcctcg tttgcatccg tgatgctgct ctacccttac ctgcaacgcg ctttcgacgg
     1621 cgtggtcaaa ggtttggaaa aaatcccgat gtggctgtgg tttatccccg tttccgccgt
     1681 cctgctgctg attatcgtcc tcgccccctc ggggataccc ggctttattt atgccaattt
     1741 ttaagggttt ggacatgaaa aactttcttt cccttttcgc ctccatattg atgtctgccc
     1801 tgattgccgt gtggttcagc caaaacccca tcaacgccta ctggcagcag acctaccacc
     1861 gcaacagccc gctcgaaccg ctcgccgcct acggatggtg gcggagcggt gtggcgttgc
     1921 aagaaaacgc ctacgccctt tcagacggca tcaaagcctt cctgtccggc gaaacgccgc
     1981 cgacggctca agacggcggt tcggcagata tgccgtctga agccgccgca tcagaagccg
     2041 tccctcaaac cggtgaaaca gaatggaaac aagacaccga agccgccgcc gtccgcagcg
     2101 gcgacaaagt cttttttgcc ggcgactcgc tgatgcaggg cgttgcaccc ttcgtgcaaa
     2161 aaagcctgaa acagcaatac ggcatcgaat ccgtcaacct cagcaaacaa agcacggggc
     2221 tgtcctaccc ctcattcttc gactggccga aaacgattga agaaaccctg aaaaaacatc
     2281 ccgaaatcag cgtgctcgcc gtcttcctcg gcccgaacga cccgtgggat ttccccgtcg
     2341 gcaaacgata cctcaaattc gcttccgacg aatgggcgca agaatacctg aaacgcgtcg
     2401 accgcatcct tgaagccgca cacacgcacc gcgtccaagt cgtctggctc ggcatcccct
     2461 atatgaaaaa agccaagctc gacgggcaga tgcgctacct cgacaaactg ctttcggaac
     2521 acctggaagg caaaatcatc ctgattccca ccgcgcacac cctgagcggc ggggaagacc
     2581 gctacaccga ctccgtcaac gtcaacggca aacccgtccg ctaccgcagc aaggacggca
     2641 tacactttac cgccgaagga caaaaactgc tggcggcaaa aataatggaa aaaatcgttt
     2701 ttgaaccaag tacgcaacca tcaagtacac agccatgaac cccaaacacc tcatcgcatt
     2761 ttccgccctg ttcgccgc
//
LOCUS       DAWHXZ010000217         2769 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000217 DAWHXZ010000000
VERSION     DAWHXZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.217"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            344..1453
                     /gene="proB"
                     /locus_tag="WIE35_001852"
     CDS             344..1453
                     /gene="proB"
                     /locus_tag="WIE35_001852"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ4154731.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGSALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            1466..2728
                     /locus_tag="WIE35_001853"
     CDS             1466..2728
                     /locus_tag="WIE35_001853"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ4154732.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALFAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYVDKDADLEKAIRIVRNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIETHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT          700 a          760 c          768 g          541 t
ORIGIN      
        1 gtcgtcgcgg cattttcggg atcgtgctgt ttcaacgctt cggcaagcgc gttcacgccg
       61 ccgaaataag caaccaagcg gtctatatct aattgcatgg tagtccttgt ctaaaataaa
      121 tcagcctaca gcagaaatat ggacaggatt atacgcattt tggacaaaac ggcaatataa
      181 atttgaaaaa tctcaataag ccgccataga ttttattaat tttatacatt ttgtctattt
      241 tatcgcagac gcggcgtgat tgttgacaat tgcggcagaa cagggcaaat tcggcggcac
      301 aaggcggcaa acgagacagg acaaaccaac aaggacggaa acaatgaaat acaaaagaat
      361 cgtatttaaa gtcggcacat cttcgattac ccattcggac ggcagtctct cgcgcggcaa
      421 aatccaaacc atcacccgcc agcttgccgc attgcatcat gcgggacacg agctggtctt
      481 ggtgtcttcc ggcgcggttg cggcagggtt cggtgcgctg ggtttcaaaa aacgtccggt
      541 caaaatcgcc gacaaacagg cttccgccgc cgtcgggcag gggctgctga tggaagaata
      601 tacggcaaac ctgtcttcag acggcatcgt gtccgcacaa atcctgctca gccgcgccga
      661 ctttgccgac aaacgccgct accaaaatgc cggcagcgca ctttccgtgc tgctgcaacg
      721 ccgcgccgtc cccatcatca atgaaaacga tacggtttcg gttgaggaat tgaaaatcgg
      781 cgacaacgac acattgagtg cgcaagtggc ggcgatgata caggcagacc tcttggtgct
      841 gctgaccgac atagacggtc tttacacggg caacccgaac agcaatcccg atgccgtacg
      901 gctggacaaa atcgaacaca tcaaccatga aatcatcgaa atggcgggcg gctcgggttc
      961 ggcaaacggc acaggcggta tgctgactaa aatcaaagcg gcgacgattg cgaccgagtc
     1021 cggcgtaccg gtctatatct gttcctcgct caaacccgat gcacttgccg aagcggcaga
     1081 taatcaggcg gacggctcgt ttttcgtccc ccgtgccaaa ggtttgcgga cgcagaagca
     1141 atggctggcg ttctattccg aaagccgggg cggcgtttat gtggacgaag gtgcggaaca
     1201 cgctttgtcc gaacagggaa aaagcctgct gatgtcgggc attgccggaa tcgaagggca
     1261 tttttcccgt atggacaccg taaccgtgta cagcaaggca accaaacagc ccctgggcaa
     1321 agggcgcgtc ctgttcggtt ctgccgccgc cgaagacctg ctcaaatcgc gtaaggcgaa
     1381 aggcgtgttc atccatcggg acgactggat ttccatcacg cccgaaatac gcctgcttct
     1441 gaccgaattt tagaaaagga aacccatgtc aaacacacaa aaacagcttg cccttgccaa
     1501 agcggcaaaa aaatccgtca acacggcgga tacggaagaa aaaaaccgcg cgctgtttgc
     1561 tatggcggac agcttggaag cggcggcggc agatattttg gcggcaaacc gtcaagactt
     1621 ggaagccgcg gcaggcaaca ttcccgaaag catgaccgac cgccttttgt tggacggcaa
     1681 acgcatttgc gcgatggcgg acggcatcag ggcggttgcc gcgctgcccg accccgtggg
     1741 cgaaatactg gaaacctcga ctttgcccaa cggcttggaa atcgtcaaaa agcgcgtggc
     1801 gatgggcgtt atcggcatta tttacgaaag ccgcccgaac gttacttccg atgcggcggc
     1861 tttggcactg aaaagcggca gcgcggtcgt actccgcagc ggcaaagatg cattccaatc
     1921 cgcacgcgcc atcgttgccg ccctgaaaac ggggttggca caaacccgca tcgaccccga
     1981 cgcgctccag ttgattgaag acaccgggcg cgagggcagt tacgaaatga tgagggcgaa
     2041 agattatcta gacctgctga ttccgcgcgg cggggcgggg ctgatacggg cggtggttga
     2101 aaatgccgtc gtgccggtca ttgaaacggg aacgggcatc gtccacatct acgtcgataa
     2161 ggatgcggac ttagaaaagg caatccgcat tgtccgcaac gccaaaacca gccgtccgtc
     2221 cgtgtgcaac tcgatggaag tgctgctggt acatgaagac attgctgccg acttcctgcc
     2281 caagctcgaa cggctgttgg ttcgcgaccg tatagaagcc ggactgccgc ccgtccgctt
     2341 ccgtttggat ccgcaggcgg cgcggcatat cggcggcgaa gcggcgggtg cagacgattt
     2401 cgataccgag tttttagact acatcctcgc cgtgaaaacc gtcgcttcgg tcgaagaggc
     2461 ggtcgggcac atcgaaaccc acggcacgca ccattcagac ggcatcgtta ccgaaaaccg
     2521 ccacgctgcg gactatttca cgacccatat cgattctgcc gccgtgtatg tcaacgcgtc
     2581 cacgcgcttt accgacggtg gcgaattcgg cttgggttgc gaaatgggca tctccacgca
     2641 aaaactccac gcccgaggcc cgatgggctt gaaagagcta acgagttata aatacatcgt
     2701 acaaggcacg ggacaggtca gggaataatc cgaattcaaa cgtctgatat agtggattaa
     2761 ctttaaacc
//
LOCUS       DAWHXZ010000218         2766 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000218 DAWHXZ010000000
VERSION     DAWHXZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.218"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            126..449
                     /locus_tag="WIE35_001854"
     CDS             126..449
                     /locus_tag="WIE35_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ4154733.1"
                     /translation="MQNETINPKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSVVLIELSD
                     PSEAV"
     gene            563..2766
                     /locus_tag="WIE35_001855"
                     /pseudo
     CDS             563..2766
                     /locus_tag="WIE35_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
BASE COUNT          668 a         1001 c          692 g          405 t
ORIGIN      
        1 gtaagccgca ggcttacatc aaagccgata acgcttccgt tacaactccg cccactgaaa
       61 gcagcctgca acgaagccaa aacgacaaac cgcatcgtaa accacccaac ccataggaga
      121 accccatgca aaacgaaacc atcaacccga aacagcacct tgccgccatc aaagaatact
      181 ggcagcccga aatcatcaac cgccacgggt tccaattccg cttggtcaaa cttttgggcg
      241 attacggctg gcatacgcac ggatacagcg acaaagtgct gtttgccgtg gagggcgaca
      301 tggcggtgga cttcgccgac ggcggcagca tgacgatacg cgagggcgag atggcggtcg
      361 tgccgaagtc ggtgtcgcac cgcccgcgtt cggaaaacgg ctgctcggtg gtgctgattg
      421 agttgtccga cccgtccgag gccgtctgaa aacgaagttt ccgaaggaag ctgagtttct
      481 gcgaagctaa aagcagcctg caccttcaat caatatgccg aaaatacaac cccaccgcac
      541 accaacacac aaaggaaatc ccatgacacg cttcaaatat tccctgctgt ttgccgccct
      601 gttgcccgtg tacgcgcagg ccgatgtttc tgtttcagac gaccccaaac cgcaggaaag
      661 cactgaattg ccgaccatca ccgttaccgc cgaccacacc gcgagttcca acgacggcta
      721 cactgtttcc ggcacgcaca ccccgctcgg gctgcccatg accctgcgcg aaatcccgca
      781 gagcgtcagc gtcatcacat cgcaacaaat gcgcgaccaa aacatcaaaa cgctcgaccg
      841 cgccctgttg caggcgaccg gcaccagccg ccagatttac ggctccgacc gcgcgggcta
      901 caactacctg ttcgcgcgcg gcagccgcat cgccaactac caaatcaacg gcatccccgt
      961 tgccgacgcg ctggccgata cgggcaatgc caacaccgcc gcctatgagc gcgtagaagt
     1021 cgtgcgcggc gtggcggggc tgctggacgg cacgggcgag ccttccgcca ccgtcaatct
     1081 ggtgcgcaaa cgcccgaccc gcaagccatt gtttgaagtc cgcgccgaag cgggcaaccg
     1141 caaacatttc gggctgggcg cggacgtatc gggcagcctg aacaccgaag gcacgctgcg
     1201 cggccgcctg gtttccacct tcggacgcgg cgactcgtgg cggcagcgcg aacgtagccg
     1261 cgatgccgaa ctctacggca ttttggaata cgacatcgca ccgcaaaccc gcgtccacgc
     1321 aggcatggac taccagcagg cgaaagaaac cgccgacgcg ccgctcagct acgccgtgta
     1381 cgacagccaa ggttatgcca ccgccttcgg cccgaaagac aaccccgcca caaattgggc
     1441 gaacagccgc caccgtgcgc tcaacctgtt cgccggcatc gaacaccgct tcaaccaaga
     1501 ctggaaactc aaagccgaat acgactacac ccgcagccgc ttccgccagc cctacggcgt
     1561 agcaggcgtg ctttccatcg accacaacac cgccgccacc gacctgattc ccggttattg
     1621 gcacgccgac ccgcgcaccc acagcgccag cgtgtcattg atcggcaaat accgcctgtt
     1681 cggccgcgaa cacgatttaa tcgcgggtat caacggttac aaatacgcca gcaacaaata
     1741 cggcgaacgc agcatcatcc ccaacgccat tcccaacgcc tacgaatttt cccgcacggg
     1801 tgtctacccg cagcctgcat cgtttgccca aaccatcccg caatacggca ccaggcggca
     1861 aatcggcggc tatctcgcca cccgtttccg cgccgccgac aacctttcgc tgatactcgg
     1921 cggcagatac agccgttacc gcaccggcag ctacgacagc cgcacacaag gcatgaccta
     1981 tgtgtccgcc aaccgtttca ccccctacac aggcatcgtg ttcgacctga ccggcaacct
     2041 gtctctttac ggctcgtaca gcagcctgtt cgtcccgcaa tcgcaaaaag acgaacacgg
     2101 cagctacctg aaacccgtaa ccggcaacaa tctggaagcc ggcatcaaag gcgaatggct
     2161 tgcaggccgt ctgaacgcat ccgccgccgt gtaccgcgcc cgtaaaaaca acctcgccac
     2221 cgcagcagga cgcgacccga gcggcaacac ctactaccgc gccgccaacc aagccaaaac
     2281 ccacggctgg gaaatcgaag tcggcggccg catcacgccc gaatggcaga tacaggcagg
     2341 ttacagccaa agcaaaaccc gcgaccaaga cggcagccgc ctgaaccccg acagcgtacc
     2401 cgaacgcagc ttcaaactct tcactgccta ccactttgcc cccgaagccc ccagcggctg
     2461 gaccatcggc gcaggcgtgc gctggcagag cgaaacccac accgaccctg ccacgctccg
     2521 catccccaac cccgccgcca aagcccgcgc cgccgacaac agccgccaaa gcccgcgccg
     2581 ccgacaacag ccgccaaaaa gcctacgccg tcgccgacat catggcgcgt taccgcttca
     2641 atccgcgcgc cgaactgtcg ctgaacgtgg acaatctgtt caacaaacac taccgcaccc
     2701 agcccgaccg ccacagctac ggcgcactgc ggacagtgaa cgcggcgttt acctatcggt
     2761 ttaaat
//
LOCUS       DAWHXZ010000219         2753 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000219 DAWHXZ010000000
VERSION     DAWHXZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.219"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(69..650)
                     /gene="petA"
                     /locus_tag="WIE35_001856"
     CDS             complement(69..650)
                     /gene="petA"
                     /locus_tag="WIE35_001856"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ4154734.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(773..1522)
                     /locus_tag="WIE35_001857"
     CDS             complement(773..1522)
                     /locus_tag="WIE35_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ4154735.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            1653..2582
                     /locus_tag="WIE35_001858"
     CDS             1653..2582
                     /locus_tag="WIE35_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ4154736.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAEWDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNDIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
BASE COUNT          627 a          774 c          639 g          713 t
ORIGIN      
        1 tccatccagc ctaacaatgc ttttgctttg ctattggttt ggtttgccat aattatcgtt
       61 ccttattctt agtcttcgcc caccaagata gttgtgtcgc tcaagtattt atatggcggg
      121 acaaccaggt tggtcggggc aggaacacct ttatatacgc ggccggccaa gtcgaatttc
      181 gaaccgtggc aggggcagaa gaagccgcct ttccagtctg cacccaaatc ggcaggggca
      241 atgtcgggac ggaaggtggg cgagcagccc aaatgggtgc agataccgat ggcgacaagg
      301 atgttcggct taatcgaacg ggtctcgttt ttagcatact ccggctgctg ttccgcatcg
      361 gaattgggat cggtaagttc gccgttcagg cctttcaggt ctttaagctg ctgatctgta
      421 cggttgagca cccaaatcgg tttgccttgc cactcggcgg tcagcagctg acccgcttcg
      481 attttactga catccacctc gacggcagca ccggcggcct tggctttttc cgaagggaaa
      541 aaactggcca caaacggcgt tgccacaccc aatgctgcca ctccgcccgc gccgcaggtc
      601 gcgagtgtca ggaaacggcg gcggccgttg ttgatttctt gattatccat tattcagtcg
      661 tcctaatatt ttgggaatac cgagccatta aacgttgcaa ttttacccag tttgcagtga
      721 tactcaaagc attatttaaa ataaggtaaa gttttatgat atttctcaag actcaagccg
      781 gattgttttc gtcaaaatgg cacacttcca acccgaaaac ctctgccgcc gattctgcca
      841 gtgcgcgtac gccgtaacgt tccgtcgcgt gatgccctgc cgaaatgaaa gccgtacccg
      901 tttcattggc gagatggtat tgggcttcag agatttcccc cgtcagatac agatcgacac
      961 cttcgtctat tgccgtctga aaaaacccct gcgccccgcc gctgcaccat gcaacccgtc
     1021 ggatttcgcg ttcgggtttg ccgatgacga caggcttacg ttgcaaaact gtttcaatat
     1081 gcgccgccaa tgcgccgagt gtcttggctt gtttcaggct gcccgagttg agcaggtttt
     1141 gttcgccgaa ccgtttttct gtcgcaaaac ccaatctgtc ggcgagttgg gcattgttgc
     1201 ccagtgtggg atgtgcatcc aggggcagat ggtagcctgc catattgatg tcgtgccgta
     1261 acagtgcggc aatccgttct tttttccaac cagtaacggt cggcaactcg tttttccaaa
     1321 acataccgtg atgtaccaaa agcaaatctg ccttctgctc cacagcaaaa tcaatcgctg
     1381 ccctgcttgc cgttaccgac gtaacgattt tccctatata ttccctccct tcaacctgca
     1441 aaccgttagg ggcgtaatct ttaaacaacg ctgtctgcaa tgtttcatta caccaagtca
     1501 gaaaatccct gcgcaatacc atcttttttc ctaatcgctt taaacaagcg ggcattctaa
     1561 tcgcaaaatg tccggaattc acatttttcc gatttgcacc cgcatatgaa ttattttaat
     1621 atgcgccggt tcaatatgcc gtctgaagcc ccatggattc cattatcgaa ctgcgccacc
     1681 tcaagaccct gctggcactt gaagaaaccg gcagcgtctc ccttgccgcc aaacgggttt
     1741 tccttaccca atccgctctt tcccaccaga tccgtatgct cgaaaaccac tacggcacgc
     1801 cgctgttcga acgcaaatcc acgcccttgc gctttacccc ggtgggcgaa aggctgctgc
     1861 gcctcgccca cgaacttata cctcaagttg ctgttgcaga atgggatttg gcgcgaatca
     1921 cggaaggaga ggcgggagag ctgcggattg ccgtcgaatg ccatacctgt ttcgactggc
     1981 tgatgcccgc catgggcgaa ttccgcccga tgtggcccca agtcgaattg gatatcgtat
     2041 cgggattcca agcggatccc gtcggactgc tgctgcaaca ccgtgccgac cttgccattg
     2101 tttccgaagc ggaaaaacaa aacgatattt ctttccaacc gctgtttgcc tacgaaatgg
     2161 tcggcatttg cgcaccagac catccgcttg ccgccaaaaa cgtttggacg gcggaagact
     2221 ttatcgggga aaccctgatt acttatcccg ttcccgacga gatgctggat ttgcccaaaa
     2281 aaatcctgat tccgaaaaac atcaacccgc cgcgccgaca cagcgaactg accatcgcca
     2341 ttatccaact ggttgccagc agacgtggca ttgccgccct tccctattgg acagtcatgc
     2401 cctaccttga aaaaggctat gtcgtccacc gccaaattac tgccgacgga ttgcaaagca
     2461 aactgtatgc cgccatccgt accgaagata cggacaagag ctatctgaac aatttttgcc
     2521 aaatcatacg cgaacgcggt tttgcagatt tgcccggact gagcgaattg gaaccggtct
     2581 gaccccttat tcaaccatac ccggcagttt ttctattttt tcatgtatag tggattaaca
     2641 aaaaccagta cagcgttgcc tcgccttgcc gtactatttg tactgtctgc ggcttcgtcg
     2701 ccttgtcctg atttttgtta atccactata ctgtttttga tttttgccca atc
//
LOCUS       DAWHXZ010000220         2738 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000220 DAWHXZ010000000
VERSION     DAWHXZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.220"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..2073
                     /locus_tag="WIE35_001859"
     CDS             <1..2073
                     /locus_tag="WIE35_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ4154737.1"
                     /translation="AGQDGKAEMYSASVSTYPDSSSSRIYLQNLKRKTDSNRPGRYSL
                     ADLSASDIQSKEPTFTSRQTIIRLDGGVRHIQLDRNNEVTSFNGDNGTFGIVNEGSVV
                     PESNEWKKVLLPWTVRGFADDSKFKAFNKEKNNDNKPKYSQRYRIRENGNNGKRDLGD
                     IVNSPIVAVGEYLATSANDGMVHIFKKGNGGDARDYSLKLSYIPGTMPRKDIQSQDST
                     LAKELRAFAEKGYVGDRYGVDGGFVLRQVNNLNEQDRVFMFGAMGFGGRGAYALDLTK
                     ADGNDPTKASLFDVKDNGNNGNNGNNRVELGYTVGTPQIGKTHNGKYAAFLASGYATK
                     EITSGENKTALYVYDLENNNGTPIAKIEVPNGKGGLSSPTLVDKDLDGTIDIAYAGDR
                     GGKMYRFDLSSQSPDQWTVRPIFEGTKPITSAPAISQLKDKRVVIFGTGSDLSEDDVD
                     KMDEQYIYGIFDDDTETTGNVKVDLKGLGGGLLEQHLTQEDKTLFLTDYKRSDGSGNK
                     GWVVKLKDGQRVTVKPTVVLRTAFVTIHKYTGNDKCGAKTAILGINTADGGKLTKKSA
                     RPIVPDANTKVAQYSGDKKTSSGKSIPIGCMQKGNEIVCPNGYVYDKPVNVRYLDEKK
                     TDGFSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTLLMNDLDSLDITGPTCGMKR
                     ISWREVFF"
     gene            2448..2732
                     /locus_tag="WIE35_001860"
     CDS             2448..2732
                     /locus_tag="WIE35_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ4154738.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          785 a          630 c          720 g          603 t
ORIGIN      
        1 gctgggcaag atggtaaggc ggagatgtat tccgcttcgg tttccaccta ccccgacagt
       61 tcgagcagcc gcatctacct tcaaaatctg aaaagaaaaa ccgactccaa cagacccggc
      121 cgctattccc tcgcagactt gagtgcgtcg gatattcaaa gtaaagagcc gactttcaca
      181 agtcggcaaa caatcatccg cttggatggt ggcgtacggc atatccaact ggatagaaac
      241 aatgaggtca ctagttttaa tggcgacaac ggaactttcg gcattgttaa tgaagggagc
      301 gtcgttcctg aaagcaacga gtggaaaaaa gtgctgctgc cttggacggt tcggggtttt
      361 gctgatgaca gtaaatttaa agcattcaac aaagaaaaaa acaacgacaa caagccaaaa
      421 tacagccaaa gataccgcat ccgcgaaaac ggcaacaacg gcaagcgcga tttgggcgac
      481 atcgtcaaca gccccatcgt ggcggtcggc gagtatttgg ctacttccgc caacgacggg
      541 atggtgcata tcttcaaaaa aggcaacggc ggggacgcgc gcgactatag tctgaagctc
      601 agctacatcc cgggcacgat gccgcgcaag gatattcaaa gtcaagactc caccctcgcc
      661 aaagagctgc gcgcctttgc cgaaaaaggc tatgtgggcg accgctacgg cgtggacggc
      721 ggctttgtct tgcgccaagt taacaactta aatgagcaag accgcgtgtt tatgttcggc
      781 gcgatgggct ttggcggcag aggtgcgtat gccttggatt tgaccaaagc cgacggcaat
      841 gacccgacaa aagcctcttt gtttgatgta aaagataacg gcaataatgg caataacggc
      901 aataatcgcg tggaattagg ctacaccgtc ggcacgccgc aaatcggcaa aacccacaac
      961 ggcaaatacg ccgctttcct cgcctccggt tatgcgacta aagaaattac cagcggcgag
     1021 aataaaaccg cgctgtatgt gtatgatttg gaaaacaaca acggtacgcc gattgcaaaa
     1081 atcgaagtac ccaacggcaa gggcgggctt tcgtccccca cgctggtgga taaagatttg
     1141 gacggcacaa tcgatatcgc ctatgccggc gatcgcggcg ggaaaatgta ccgctttgat
     1201 ttgagcagtc aaagtcctga tcaatggact gtacgcccta tttttgaagg cacaaaaccg
     1261 attacttccg cgcccgccat ttcccaactg aaagacaaac gcgtggtcat cttcggcacg
     1321 ggcagcgatt tgagtgagga tgatgttgat aaaatggacg aacaatatat ttacggtatc
     1381 ttcgacgacg atacggagac gacgggtaat gtaaaggtag accttaaagg tttgggaggc
     1441 gggctgctcg agcaacacct tactcaggaa gataaaacct tattcctgac cgattataag
     1501 cgatccgacg gctcgggcaa taagggctgg gtagtgaaat tgaaggacgg acagcgcgtt
     1561 accgtcaaac cgaccgtggt attgcgtacc gcctttgtaa ccatccataa atatacgggt
     1621 aatgacaaat gcggcgcgaa aaccgccatt ttgggcatca ataccgccga cggcggcaag
     1681 ctgaccaaga aaagcgcgcg cccgattgtg ccggacgcca atacgaaggt cgcgcaatat
     1741 tccggcgata agaaaacttc cagcggcaaa tccatcccta taggttgtat gcaaaaaggc
     1801 aatgaaatcg tctgcccgaa cggatatgtt tacgacaaac cggttaatgt gcgttatctg
     1861 gatgaaaaga aaacagacgg attttcaaca acggcagacg gcgatgcggg cggcagcggt
     1921 atagaccccg ccggcaagcg ttccggcaaa aacaaccgct gcttctccca aaaaggggtg
     1981 cgcaccctgc tgatgaacga tttggacagc ttggacatta ccggcccgac gtgcggtatg
     2041 aaacgaatca gctggcgtga agtcttcttc tgatttgcac gcgaaaatgc cgtccgaaag
     2101 gttttcggac ggcatttttt gcgtttttcg ggaggggcgg gttcgtaaaa ggcgggctat
     2161 agggtaggct tcatctcgcc aatctcactg aatccatcaa tttccacaat tcaattaaat
     2221 accgtcaaac cgatgccgtc attcccgcgc aggcgggaat ctagaccttg ggataacagt
     2281 aatattcaaa ggttagctga agctttagag attctggatt cccactttcg tgggaatgac
     2341 gaagagttgc gggaatgatg gaaagctatg ggaataacga agggttaaag taatcacggg
     2401 atggtgttcg cgggaatata aattgaaata attcaaaagg gtattatatg cagcctgcgg
     2461 tttatatttt agcaagccaa cgtaatggca cgttatacat tggcgttaca tctgatttgg
     2521 tgcaacgtat ttaccaacat agggagcatt tgattgaggg atttacatca cggtacaacg
     2581 ttactatgct ggtttggtat gaactgcatc ctacgatgga gagtgcaatt actcgggaaa
     2641 aacagttgaa gaaatggaac agggcttgga aattgcaact gattgaagaa aataatgttt
     2701 cttggcagga tttatggttt gatattattt agcccgtc
//
LOCUS       DAWHXZ010000221         2729 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000221 DAWHXZ010000000
VERSION     DAWHXZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.221"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..1126)
                     /locus_tag="WIE35_001861"
     CDS             complement(<1..1126)
                     /locus_tag="WIE35_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEZ4154739.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     DLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKG"
     gene            complement(1119..2579)
                     /locus_tag="WIE35_001862"
     CDS             complement(1119..2579)
                     /locus_tag="WIE35_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ4154740.1"
                     /translation="MGSAVGGQPLEKIFLPDDKGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPRLDSGEAAFCPR
                     CGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKRVYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDGAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPAGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGMAFNETEKHD"
BASE COUNT          697 a          723 c          727 g          582 t
ORIGIN      
        1 ggcctttgtt taaggccgtc tgaaattgtt gtttccaatg ttctttgctt tgttcgtcgg
       61 cattgatttc caaacgggaa ggttcaatgc ggatgcgtac gggtatccag ccgttttcaa
      121 acaggtgcag gctgtcgttg cggtcgaaat aaggaacgtc ggaaaccacg ccgacattca
      181 gccctttgta ctcgacgggc gaaccgacgg tcaggccgcg cacggattgt ttgaaaaacg
      241 cggtgtagta cagcgagcgg tcgtcaggca ggttggcgac ttcgctgcgg ctgtcgtaaa
      301 gcgtgaagct gtcttcgctt ttgacgtttt tactgttttt ggttttcggc gaatcaaatg
      361 aaatcgcgcc cgacagcagg gcaggcagag gggcggaatt gagtttgatg ccgctgcctg
      421 tggtttcgat attgatgccg ctttccagcc aaaaacggct ggcggaatga atcagtttgt
      481 cgttggggct ttggatgaag atggtgtaat gcacgctttg gtcggacggg tcgaaatgcg
      541 cgctttcgac ttgcccgacc ataaagtttt catacaaaac agggctgttg acgttgagga
      601 tgcggtcgtt tttaccaatc aaattcaagc gcagcccgct ttgcccgatg gcggtaacgg
      661 gcggaatgtc ctgcacctgg aacacgtctt ttgcctcgtc gcttttgccg ggtgtaaagg
      721 cgatgtacga acccgaaagc agcgtaccca aaccggttac gccgctttgg tcgatacgcg
      781 gcttgaccac ccaaaactgg gtatcgctgc ggatgaggtc ggatacgtcc gcattgagtt
      841 gggcggttac ttccacgcct ttttggtcgt cgcgcagttt gattcgggta acgcgtccga
      901 catcgatgct caatactttg atgaccgtat tgttgacctc aatgccttcc gcgctgtcca
      961 tcaagagcgt aaccacaggc cccctgttgc ggatttcctt aacccaaagc cagccgccgg
     1021 caatcagcgc gatcagcgga accagccaga cggcggagag caaggtgttg tttttgcgga
     1081 cgcgtgcttg ggcgtgtccg tttggaggag ggctgttgtc agtcatgttt ttccgtttcg
     1141 ttaaaagcca tgccgtctga agcgcgtttg tcccaaagca ggcgcgggtc gaaataatag
     1201 gcggacagca tcgtcagaat cacgaccagg cagaaataga ctgccgcact gcccggaatg
     1261 acgcgcgcgg cataagtgtg gaacgaacac atcaaaataa taatcacaaa aatatcaatc
     1321 atcgaccagc gaccgactgc ttcggtgatg cggtagaggt gcgacaattt ctttgcgccc
     1381 gccggcaaag cgaagcgggc ggacgcaatc aaaaccgaca ttgccgcaat cttcagcacc
     1441 ggcaccaaaa tactcgcgct gaaaataacc gccgcaatca gcctgtcgcc ctcgtcccac
     1501 atataagcga tgccgttaag gatggtattg acctccgtgg cggcaggatt ggatgaaatc
     1561 ataatcggca ggatattggc agggaaatac aaaataaccg ccgccgtcag aaacgccgac
     1621 gaaatactca gacttttcgg ccgtcggcgg tacagttccg caccgcacac gccgcagggg
     1681 gattcggcac cgtctcggaa atacaggcag cggctgcaac aggttttgcc ttctgatgcc
     1741 gtctgaaccg cattatcccc cgtcagccgc ccgatttgaa aatacaccca atgctgggga
     1801 accgataccg aagtccgaat cagcataacc gacagcgcga acatcagata aaacgccgac
     1861 ccgaagcgaa cctctgccac agacgagagc ttgatatacg ccaccagcgt ggaaacaaaa
     1921 aacacatcca ccatcatcgc ctgcctcaag cgcaccatca cacgcgttgc caaacgcagc
     1981 gcaggataca cccgtttccg tatcagcgcg gcatagacat acaggcacag cagcagaaac
     2041 agaaccggcg cgccgaaggt cagcacaaac atcacttcgg ccaaaaaacc ataatcctga
     2101 aacaccatca ggcgcatcat ctcgggcagc gaaaggacgg atgccgcacc cggtatcccg
     2161 acctcgatat acgtcatacc gtaagcaaac gccatcaaaa tcagcgaagc cgccgcataa
     2221 gcgggcgggg cggaaaaagg atgcctgccc accctgaaga gcttatagcc gcagcgcggg
     2281 cagaacgccg cttctccact gtccaaccgg ggtacatccg cgcggcagcc gcattccgga
     2341 caatctaccg tatgcggcgg tagggcttcg tcgcggcagt tgccgcgaag gctgtggggg
     2401 atattttcgg caaacggctt catcgggaaa gtggcaaata taagtggcgg cattataaag
     2461 gaaaaaaggc gatacaccca atcaggatgc aaaaccggca ggttcggcag tatgaacaaa
     2521 cccttgtcgt ccggcaaaaa tattttttcg agcggctgtc cgccgactgc cgaacccatt
     2581 tgataaaatc ccgattctgt aaagaatagt aaaatacctg cttaaaaaca tcacgaaaat
     2641 gatatttttt ccaaaaatat gtgataataa atttcgttta tgattcttta ttatttttat
     2701 agtggattaa caaaaatcag gacaaggcg
//
LOCUS       DAWHXZ010000222         2689 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000222 DAWHXZ010000000
VERSION     DAWHXZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.222"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1232
                     /locus_tag="WIE35_001863"
     CDS             <1..1232
                     /locus_tag="WIE35_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ4154741.1"
                     /translation="FWYYFGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTA
                     VEYIMRDVSGGWIIRYMHSTGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFL
                     ALMAEAFMGYLLPWGQMSFWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFF
                     ALHVIAVPLVLLGLVVAHIIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVK
                     DILGVVVFLIVFCAVLFFAPEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILR
                     AIPSFLGTQVWGVIGMGAAVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGL
                     GILGAMVATDTRTLVARILSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFF
                     VYVGITAVGAYLFATNI"
     gene            1247..2035
                     /locus_tag="WIE35_001864"
     CDS             1247..2035
                     /locus_tag="WIE35_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ4154742.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            2374..>2689
                     /locus_tag="WIE35_001865"
     CDS             2374..>2689
                     /locus_tag="WIE35_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ4154743.1"
                     /translation="MELMTVLLPLAALLSGVLFTWLLMKSRFQGVLAVLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYEHLQDENYALGNRFSAAEKQIAYLQEKEAESVRLK
                     QSY"
BASE COUNT          621 a          627 c          692 g          749 t
ORIGIN      
        1 atttttggta ctacttcggc tctttggccc tgcttgtcct cgtgattcaa atcgtcagcg
       61 gcattttcct gaccatgaac tacaaacccg acggcaacct gaacgcctac catctgcctg
      121 ctgcctttac tgcagtcgag tacatcatgc gcgacgtgtc cggcggttgg attatccgct
      181 acatgcattc caccggcgca tctttcttct tcattgttgt ttatctgcac atgttccgtg
      241 gtttgattta cggttcgtac aaaaaacccc gtgagttggt gtggattttc ggttctctga
      301 ttttcttggc attgatggcg gaagccttta tgggctacct gctgccttgg ggtcagatgt
      361 ccttttgggg tgcgcaggta attattaacc tgttctccgc catccctgtt atcggtccgg
      421 atttgtccac ttggattcgc ggcgacttca acgtttccga cgttaccctg aaccgcttct
      481 ttgccctgca cgttatcgcg gtacctttgg tattgctcgg cttggttgtg gcgcacatca
      541 tcgccctgca cgaagtgggt tctaacaacc ctgacggcgt tgaaatcaag aaaaacaaag
      601 acgaaaacgg tatcccgcgc gacggtatcc cattccatcc ttactatacc gttaaagaca
      661 tcttgggtgt ggttgtattc ctgattgtct tctgtgccgt attgttcttc gcaccggaag
      721 gcggcggcta cttcctcgaa gcgccgaact tcgatgcggc aaatgcgctg aaaacacctc
      781 cgcacattgc gccggtatgg tatttcacac cgttctacgc cattttgcgt gcgattccct
      841 cgttcttggg aacgcaggtt tggggtgtaa tcggtatggg cgcggcggtc gtgttgattg
      901 ccctgctgcc ttggctggat aggggcgagg tgaaatccgt ccgttaccgc ggcccgattt
      961 tcaaaaccgc attggtcttg ttcatcattt cctttatcgg tttgggtatt ttgggcgcga
     1021 tggtggcaac cgatacgcgt actttggtcg cgcgcatcct gtccttcgtc tatttcgcat
     1081 tcttcctggg tatgccgttc tataccaagc tggataaaaa caaaccggtt cccgaacgcg
     1141 taaccatgag caccgccaaa caaaaaatca tgttctttgt ttacgtcggc attaccgccg
     1201 tgggtgctta cctgtttgca accaacatct gatgagggca gtcaaaatga aacaagctat
     1261 gaaaaattgg tttgctgcct tgctgttggc agtacccatg ggtgcggcat ttgcttcggg
     1321 cggtcatgca cactatgaga aagtcgatat cgatttgcgc gaccaagtca gcctgcagcg
     1381 cggcgcacaa atctttacca actattgcct gtcctgccac tcggcaagcg gtatgcgttt
     1441 caaccgtttg aaagacatcg gtttgactga cgaagaaatc aagaaaaacc tgatgtttac
     1501 caccgataat gtcggcgatg tcatgcattc ggcgatgaac ccgaaagatg cggcaaaatg
     1561 gtttggtgct gctccgcccg atttgacatt gattgcgcgt tccaaaggtg cagactacct
     1621 ttacgcttat atgcgcggct tctataaaga tccgacccgt ccgagcggct ggaacaatac
     1681 tgtattcgat aaagtcggta tgccccaccc gttgtgggaa cagcaaggcg ttcaagccgt
     1741 tgagttggat gccaaaggtc agccggttat ggtaaaagac gaacacggcg agatgaagcc
     1801 taagctgtat tgggaatcta ccggtttgca cagccgccgt ctgcctaacg gcaaagtgat
     1861 ccaaaaagag tacgacgcat atgtacgcga tttggtcaat taccttgtgt acatgggcga
     1921 acctgcacaa ctgcaacgca aacgtatagg ctatgtcgtg atgattttcc tatttgcggt
     1981 tatgctgcct ttggcgtatt tcctgaaaaa agaatattgg aaagacgtac actaagcgtt
     2041 tggaacaaaa gggcaaatcc tttagggttt gccctttttt cattttgcct gccgtttgaa
     2101 aagcctgaat ccgtatgccg tctgaacata gctgcaacat ttcagacggc atcattctaa
     2161 aaatgtcaga catcgggaac ttaatcaggg tttgtggcag attgatattg aattaggaaa
     2221 aggtctgaaa ccgcaagcag catggtaagg cgatgtcgaa caggtttaaa gccaatccgc
     2281 tatatttttt ggagctatgt cgaacaaaca ggtagaatcg gaataagcgg cggttttgtc
     2341 cggtgtgaaa atgtttgaat attaggataa ttgatggaac tgatgactgt tttgctgcct
     2401 ttggcggcgt tgctgtcagg cgtgttgttt acatggttac tgatgaagag ccggtttcaa
     2461 ggtgtattag ccgtattgaa cgcgcagttg gcggaaaagg cggcaagatg tgattttgtt
     2521 gaacaggcac acgcagaaat tgcatcggaa ttggccgttt tggatgggaa atacgagcat
     2581 ttgcaggacg aaaattatgc tttgggcaac cgtttttccg cagccgaaaa gcagattgct
     2641 tatttgcagg aaaaagaggc ggaatcggtt cggctgaagc agtcgtata
//
LOCUS       DAWHXZ010000223         2683 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000223 DAWHXZ010000000
VERSION     DAWHXZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.223"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..958)
                     /locus_tag="WIE35_001866"
     CDS             complement(<1..958)
                     /locus_tag="WIE35_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ4154744.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARAD
                     AKIILEGIKTTIHNETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTEIEKLAKQPEYAYLKQLQTVKDVNWNQVQLAYD"
     gene            complement(1103..1534)
                     /locus_tag="WIE35_001867"
     CDS             complement(1103..1534)
                     /locus_tag="WIE35_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154745.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTNCSLHET"
     gene            complement(1550..>2683)
                     /locus_tag="WIE35_001868"
     CDS             complement(1550..>2683)
                     /locus_tag="WIE35_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HEZ4154746.1"
                     /translation="EIVGEALTNGKNPDTLTAKEREQILAYSKLVAGTVSGVVGGDVN
                     AAANAAEVAVKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKYQNVADKRLAAS
                     IAICTDISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFSKSYTQADLALQ
                     SYHLNTAAKSWLQSGNTKPLSEWMSDQGYTLISGVNPRFIPIPRGFVKQNTPITNVKY
                     PEGISFDTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVKSETQTDIEGIT
                     RIKYEIPTLDRTGKPDGGFKEISSIKTVYNPKKFSDDKILQMAQNAASQGYSKASKIA
                     QNERTKSISERKNVIQFSETFDGIKFRSYFDVNTGRITNIHPE"
BASE COUNT          636 a          530 c          575 g          942 t
ORIGIN      
        1 tgtcgtaagc gagctgtact tggttccagt tcacgtcctt gaccgtctga agctgtttca
       61 ggtaggcgta ttcgggctgt ttggccagct tttcgatttc ggttttcaga ttgcctttgg
      121 gaatatcgac aatgtaaccg ccgggtgcgg acagtacggg cgcaacggga ccggtgaaac
      181 tcggcaattg caaggtttcg atgttactgc cccgtcctgc ctgtttttgc catagagcag
      241 atttgctgct gctcacggtt tcgttgtgga tcgtggtttt gatgccttcg aggataatct
      301 tggcatctgc ccgtgcctgc tcacctacgc ctgcgcgtat ggttgcgcca cccagtgtgg
      361 tttcaaactg tgtgccttgc agtttggtat cccagcccga ttggaggttg gctgattctg
      421 caactaccct tgagggcagc gcggttttca tgacttgggt ggtggtgtcg tgtgctttgc
      481 tgtagctgat gccgagaaat ctgcgccttt tttggctgtc tagtttgtcg tagttgagct
      541 cttctacggc atagagtgtc aatttccgcc cggcttcgat gttaatgctg cctttggggg
      601 catcgaatgc ggtggcgtag gcgtcgatgc tgccaccaga tttgatgtcg atgccttggg
      661 atgcgctgag gtttactgcg tcgggcttgg cgtttttgtg ttctttgact tcggtaatgt
      721 gtttgcggtt gtaccatttg ccagttttgt agctgcggcg ttcaaaggta tagagttcgc
      781 tctgtctcgc atagtaatat tggtcaccgt aagattgaat tttgattttg ccgttttccg
      841 aactgatatc cgtggtgctg agcaggatgc gactgttctc attggcatac ggtgcgctaa
      901 tgctcacacc ggttttaccc gaaagttctg cagcaagcgt agggggtgtc ttcagcacgc
      961 acgcgtctgg gcagtatata gggaatctga atatttactt gcataacaaa tgccgtctga
     1021 aaaattgtga gcttttcaga cggcattgag ccgtaaatca tggaacgcgt gcgtgctgaa
     1081 gcacacacct tacgcatgga ttttaggttt catgcaggct acagtttgtt tgagaatatg
     1141 ttttttgtat ttctttacac tttttttgat attcagggtg agctattaag aaatcagata
     1201 ttgcaatatt aaagtaatga tatgctaaat taataggtat aattaattct tcaggattga
     1261 aatcatctcc aactgaaaaa taaatagaag gagctttatc aatatactca aatccattta
     1321 cctcttcata atctccaatc catctggtag catctatttt ttcatattga gcacctatta
     1381 ctccaaaatg tccggtagtt gtaatatttt taaaacactc taaaagcgta tctttagttt
     1441 caaattggta aattgtttca aaaaaaatcg aaataacaaa atggttgtta tttatagatt
     1501 ttttatttaa gtttagaaaa atattatttt tcataatttt tcctttaaat tattctgggt
     1561 gaatgtttgt aattcttcct gtatttacat caaaatatga tctaaatttg attccgtcaa
     1621 aggtttctga gaattgaatg acattttttc tttccgatat tgatttagtt ctttcatttt
     1681 gagcaatttt agaggctttt gaatatcctt gtgaagcagc attttgagcc atttgaagta
     1741 ttttatcatc agaaaatttt ttaggattat aaacagtttt tatacttgaa atttccttaa
     1801 atccaccatc aggtttacct gtcctgtcta gtgtaggaat ctcatattta attcgggtaa
     1861 tgccttcaat atcagtttgg gtttcagatt ttacgcgtcc tcctcgtgaa tttagttctg
     1921 ccataaaatt ggtgcggtta tgggctcctt taatgccctg tttttgacta aaaccatcag
     1981 catttgccag atgtcttttt aggtttgtat cgaaactgat gccttccggg tatttgacat
     2041 tagtaatagg tgtattttgt tttacaaacc ctcttggtat tggaatgaat ctaggattaa
     2101 ctcctgaaat aagtgtataa ccttggtcgg acatccattc ggataaaggc tttgtattgc
     2161 ccgattgaag ccaagattta gcagcagtat tcaaatgata agactgtaaa gccaaatctg
     2221 cttgggtgta agatttgctg aataatttat accattcatc atctttacca attagacctg
     2281 cttcccaaga tgaatgaagg cttctactat cgatcaaatg ttgttttctg attgttctac
     2341 attcagtact acgggatata tccgtacata ttgcaatcga agcagcaagt cttttatcag
     2401 caacattttg atactttttt acagtatttt ttctgcacag ttgaggatta ttctgtttgg
     2461 cgcatgcagt catttcgtta tcaaattctc taccctcttt gtcgctaagc tgattatttt
     2521 tcaccgctac ctcagccgca ttcgccgccg catttacatc gccgccgacc acaccgctta
     2581 ccgtaccggc aaccagtttg ctgtatgcca aaatctgttc gcgttcttta gctgtcaaag
     2641 tgtcaggatt tttgccgttt gtcaaagcct ccccgactat ctc
//
LOCUS       DAWHXZ010000224         2633 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000224 DAWHXZ010000000
VERSION     DAWHXZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.224"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            86..1315
                     /gene="sstT"
                     /locus_tag="WIE35_001869"
     CDS             86..1315
                     /gene="sstT"
                     /locus_tag="WIE35_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ4154747.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            1427..>2633
                     /locus_tag="WIE35_001870"
     CDS             1427..>2633
                     /locus_tag="WIE35_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ4154748.1"
                     /translation="MFERSVIAMACIFALSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     PQNAGNTAAQGTNQAENNQVGGSQNPAPSSNPNATNGGNFGRVDLANGVLIDGPSQNI
                     TLTHCKSDSCNGDNLLSEEAPSKSEFEQLSDEDKIKKYKKDGEKFTGLVADRLQMKGT
                     NQYIIFYKPKTTSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGEVLHFHTENGRSYP
                     TKGRFAAKVDFGSKSVDGIIDSGDDLHMGKQKFKAAIDGNGFKGTWTENSGGDVSGKF
                     YGPAGEEVAG"
BASE COUNT          617 a          635 c          763 g          618 t
ORIGIN      
        1 acagccgaaa aataaaaaaa cacagagaat gagttttctt ttaaaataca acatttttta
       61 acatatcaca acataaggaa gaattatggc gttcggcaaa tccttatttc atgcgatcgg
      121 ccgcgtcagc ctggtcaggc agattgccgc cggtttggcg ttgggcatcg taatcggttc
      181 ggtttccccg caactgggct tggcggcagg cttgttcggc agcctgtttg tcggcgcgct
      241 caaagcggtc gcgccggttt tggtatttat tttggtggcg gccacaatcg cgcagcacca
      301 aaaaggcaac aaggcgcata tcaggccgat tatcgtcctt tacctcatcg gtacgttttc
      361 cgcagccctg accgccgtca tcgccggtat ggttttcccg acgcacattg ttttggcggg
      421 cgcgggcgat gtgtccgccg cgccgccttc cggcattgtg gaagtgttga aatcgctgct
      481 gatgaacctg gtcgccaacc cgattaacgc gattgccaat gccaactata tcggcattct
      541 ggcttgggct ttggttttgg gcgcggcgtt gcggaatcac ggttcggacg ttacgcggca
      601 ggtcgttgcc gatttggcgg aagcggtttc caccgtcgtg aaatggatta tccgttttgc
      661 ccctttgggc attttcgggc tggtgtcgtc cacaatcgcg gagacgggtt tcggcgcgct
      721 ggcgggttat gcgaagctgc ttgcggtgct gttgggctgt atggcgttta tcgcgctagt
      781 ggtcaatccc gccatcgtgt ggtggaaaat ccgccgcaac ccttatccgc tggtgtttac
      841 ctgcctgcgc gaaagcggcg tgtatgcctt ctttacccgt tcttccgccg ccaatatccc
      901 cgtgaatatg gctttggcga aaaaactggg gctgcacgaa gacacttatt cgatttccat
      961 cccgttgggg gcgaccgtca atatgggcgg cgcggcgatt acgattaccg ttttggctat
     1021 ggcggcggcg cacacccaag gcatacaggt tgattttgcc accgcgctgc tgttgagcct
     1081 ggtggcgacg gtcagcgcgt gcggcgcgtc cggcgtggcg ggcggttcgc tgctgctgat
     1141 tccgctggcg tgcagcctgt tcggcatcag caacgatgtc gccatgcagg tggttgccgt
     1201 cggctttatc atcggcgtga ttcaggattc ggcggaaacg gcgttgaatt cttcaaccga
     1261 cgttttgttt accgctgctg ccgatttggg ccgacagaga aaccgggcgg aataaaccaa
     1321 gctatgccgt ctgaagccca tttggcgttc agacggcata tttttacaat tttatgggca
     1381 tatggcggaa acaataccgg ctgccttgaa tatgaaggag atgatgatgt ttgaacgcag
     1441 tgtgattgca atggcttgta tttttgccct ttcagcctgt gggggcggcg gtggcggatc
     1501 gcccgatgtt aagtcggcgg acacaccgtc aaaaccggcc gctcctgttg ttgctgaaaa
     1561 agagacagag gtaaaagaag atgcgccaca ggcaggttct caaggacagg gcgcgccatc
     1621 cacacaaggc agccaagata tggcggcagt ttcggcagaa aatacaggca atggcggtgc
     1681 ggcaacaacg gacaaaccca aaaatgaaga cgagggaccg caaaatgata tgccgcaaaa
     1741 tgccggcaat acggctgccc aaggtacaaa tcaagccgaa aacaatcaag tcggcggctc
     1801 tcaaaatcct gccccttcaa gcaatcctaa tgccacgaat ggtggcaatt ttggaagggt
     1861 tgatttggct aacggtgttt tgattgacgg accgtcgcaa aatataacgt tgacccattg
     1921 taaaagcgat tcttgtaatg gtgataatct attaagcgaa gaagcaccgt caaaatcaga
     1981 atttgaacaa ttaagtgatg aagacaaaat taagaaatat aaaaaagatg gagaaaagtt
     2041 taccggtttg gttgctgata ggttacagat gaaaggaacc aatcaatata ttatttttta
     2101 caaacctaaa accacttcat ctgcgcgatt caggcgttct gcacggtcta ggaggtcgct
     2161 tccggctgag atgccgctga tccccgtcaa tcaggcggat acgctgattg tcgatgggga
     2221 agcggtcagc ctgacggggc attccggcaa tatcttcgcg cccgaaggga attaccggta
     2281 tctgacttac ggggcagaaa aattgtccgg cggatcgtat gccctcagtg tgcaaggcga
     2341 accggcaaaa ggcgaaatgc ttgctggcac ggccgtgtac aacggcgaag tgctgcattt
     2401 ccatacggaa aacggacgtt cgtacccgac caaaggcagg tttgccgcaa aagtcgattt
     2461 cggcagcaaa tctgtggacg gcattatcga cagcggcgat gatttgcata tgggtaagca
     2521 aaaattcaaa gccgccatcg atggaaacgg ctttaagggg acttggacgg aaaatagcgg
     2581 cggggatgtt tccggaaagt tttacggccc ggccggcgaa gaagtggcgg gaa
//
LOCUS       DAWHXZ010000225         2613 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000225 DAWHXZ010000000
VERSION     DAWHXZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.225"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(169..924)
                     /locus_tag="WIE35_001871"
     CDS             complement(169..924)
                     /locus_tag="WIE35_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ4154749.1"
                     /translation="MKFARLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(1104..2263)
                     /gene="nirK"
                     /locus_tag="WIE35_001872"
                     /pseudo
     CDS             complement(1104..2263)
                     /gene="nirK"
                     /locus_tag="WIE35_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
BASE COUNT          606 a          583 c          725 g          699 t
ORIGIN      
        1 gcgggaatga cggaaagtgg cgggaatgac ggttcgggca ttccttaaat tacccgtgta
       61 tcgctgtaaa tcttagagat ggcggaatat agtggattaa caaaaaccag tacggcaagg
      121 cgaggcaacg ccgtactggt ttttgttaat ccactataat tgaaggggtt atcggcttgt
      181 gcaacggaag cccaagttgt gcaagacata tttggattgc aggctggtac ggatgccgta
      241 gcggaggaag gcggcatagt tggacgagtc gctcgacccg atagacgcgc cgctgcaaaa
      301 catttgcgcg ttggcattgc cggaagaaag caggctgctg ttgaaatctt ccgtccattc
      361 ccaaatcagc ccgtgcatat cataaacgcc ccagtagttc gggcggcctt tgccgacatc
      421 gtgcaggcct ttccgtccgc cgtcggcata ccaatcgaga atagtgcggt tgtagccggg
      481 ttcgtttgag ccgtttttct gcgtggcgga agcaagtccg gcaaattccc attcgtcaat
      541 ggtcggcagg cgtttgcctt gtgcggcgca ataggcgttg gcggcaaacc aggaaacatt
      601 ggttaccggt tgttttaatt cgcccgcctt cggcgcatag ctgcggctgc cgtttttcat
      661 ccaatgcttc aggtaagcgg gttctgcctg tttggaaccg atcctgcctt tttgccattg
      721 ggggtggctg ttgacaaatt cggcaaactc ggcattggta acgggatatt tatccagttt
      781 gaacggtttg actttaatca ggccggtatc ttttttcaga taaagcgggc ggtagctgcc
      841 gccttcgatt tgaaccattt cggcagccgc cgcttgagtg ccggcgagtg ccgcacagag
      901 aaagagtaac cgggcaaact tcatatcatg cctcctgaca ggcagttaaa attgattttt
      961 cagacggcat tttaaacaaa aagccgcgaa tgccgtctga agaatcaatc ttccgaaagg
     1021 aaagattggt tgttaaaaaa ccactgccgt gcgtaattaa gtacggcggc agtggttcgt
     1081 ttgcctaaaa atgggtgttc aatttagtaa tcgctttttg caggagcaga ggctgcagga
     1141 gcagaagctg cgggagcgga agcagcagga gctgcaccgt taccggcgta agcggtatca
     1201 ctcaattttt gagtcatgat ttcagggttt tctgcacctt ctactttcaa ttgacccagt
     1261 gcgcctttgt tgaatgcgcg gaagatagag tggtcaacca aagtgtagct gcccgggatg
     1321 tcgactttga attcgacgat agcagagccg ccggcaggaa cgatggtact ttgtacgttt
     1381 tcgttaatca gtttgccgcc ttcaacataa actttgtcga agatttcacc gattacgtgg
     1441 aaggaagaca ccaagttcgg gccgccgtta ccaacgtaca tacgtacagt ttcgcctgct
     1501 ttggctttca gggcgttgtc gccggcgata gcacctacgt gaccgttgaa tacgacgtat
     1561 tcaggctgtt cggcaacagc tttgtccata tcgaacggtt gcaggccttg cgcacctttt
     1621 ttgcctttgg tgtagaagtc gccttggacg atgtagaact ctttatccac tttcggcagg
     1681 ccttctttag gctcgaccaa aatcaggccg tacataccgt tggcgatgtg cataccgacc
     1741 ggcgcgacgg cgcagtggta gatgtacaga cccggttgca gggctttgaa gctgaatgtg
     1801 gaagtgcggc ccgggcggta aaggtcgcgg ctgcaccgcc gccctgaccg gttgcggcgt
     1861 ggaagtcgac gttgtgcgga acggtagaag aaggattgtt ggaaaattcc acttcaaccg
     1921 tatcgccttc gcgtacgcgg atcatgcgac ccggaacgtc gccgtcaaat gtccagtagc
     1981 ggtattccac accgtcttcc atggtcatgg ttttttcgac ggtttccatt ttcacgcgta
     2041 ctttggcagg gtagtcgcgg tcgattgcag gaggtacttc tggagcgtgt gtggtaaccg
     2101 catcgataac gggcagttcg cctgtcggcg tttcggcggc ggtttgtgcg gcggaacttg
     2161 cggcttctgc ggaagcggca ggggcttgcg cggcaggttc gcctccgcag gcggctaatg
     2221 cgaataagga agcaatcatt gcagctaagg cttggcgttt cataatgttt tccttttgta
     2281 agaaaagtag aggaattggt taagaacttg gtgtgattat gcgtttgccc cattggtggc
     2341 attttgattt aagtcaaata attcataaaa tatgaactta aaatgatact tctaatactt
     2401 tatagctact aaaaattaca atgaataggc tgatttgctt aagggcttcg gcaaatcagg
     2461 caaaggcagg gtagttgccg actaaagaat aatgatagtt attatcatat atgtatttgt
     2521 tttatatgtg agtttctttg aacaaatgtt tatttgtgcg gtaaaccgtg ctacaatctt
     2581 taacattcat attctgtgaa ttttaaacag ctg
//
LOCUS       DAWHXZ010000226         2592 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000226 DAWHXZ010000000
VERSION     DAWHXZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.226"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            41..139
                     /locus_tag="WIE35_001873"
     CDS             41..139
                     /locus_tag="WIE35_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ4154750.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(210..1595)
                     /locus_tag="WIE35_001874"
     CDS             complement(210..1595)
                     /locus_tag="WIE35_001874"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ4154751.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNSLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRVAGVADVMEGCIK
                     RGLAADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTALTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKSTYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(1692..>2592)
                     /gene="gyrB"
                     /locus_tag="WIE35_001875"
     CDS             complement(1692..>2592)
                     /gene="gyrB"
                     /locus_tag="WIE35_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEZ4154752.1"
                     /translation="IMTDADVDGAHIRTLLLTFFYRQMPELVERGYIYIAQPPLYKAK
                     YGKQERYLKDELEKDQWLLGLALEKAKIISDGRTIEGAELADTAKQFLLAKTVIEQES
                     RFVDELVLRAMLHASPIDLTSSENADKAVAELSGLLDEKEVALERIEGHEGHRFIKIT
                     RKLHGNVMVSYIEPKFLNSKAYQTLTQTAAALKGMVGEGAKLYKGENGYDADSFETAL
                     DILMSVAQKGMSIQRYKGLGEMNPEQLWETTMDPAVRRLLKVRIEDAIAADEVFVTLM
                     GDEVEPRRAFIENNALIAQNIDA"
BASE COUNT          489 a          686 c          794 g          623 t
ORIGIN      
        1 tcttgccgga tgctgcaaac aacgccggtt tgcattcctg atggcggtgg ttttcttaga
       61 cgaacgcccg aacacgcagg aatggatagg cttggggctg gttacggcgg gcgtgttgac
      121 gctggcactg aaacggtaaa gccgcaagaa ataaatgaaa tgccgtctaa aaaactgttt
      181 tcagacggca ttttcgtttc tgtccatcct cagcactcga ccacgcgcac ggatacgggg
      241 acggcttttt tccggagcgt ggcggcgagt ccggcaagcg aggtttcttt gtaggtggac
      301 ttcatgtcgc gccctgtctg taacatggtt cggatgactt cgtcgagcga gacttttttg
      361 tccgtgccgt cttccaaaag cgcgagcgtg ccgagtttga gggctttttc ggcggcgatg
      421 ccgttgcgct cgatgcaggg gatttgcacc agtccgccga cggggtcgca agtcagcccc
      481 aaatggtgtt ccatcgccat ttcggcggcg ttttccactt gtttgggcgt gccgccgatg
      541 acttcggcgt atgcgcccgc cgccatcgaa cacgctacgc cgacttcgcc ctgacagccg
      601 acatccgcac cggaaatgga ggcgttggtc ttgtagagga tgccgattgc gcctgcggtg
      661 agcaggaagt tttcgacgcg ttcctgtgtg gcgtgcggat tgaacttgcg gaaatagtgc
      721 aatacggcgg gaatgatgcc tgccgcgccg ttggtcagtg cggtaacgac gcgtccgccg
      781 gcggcgtttt cttcgttgac cgccatggcg tacaccatcg gccagagctg ggtgttgacg
      841 atttcggttt cgcgcaggac tttgagcttg gcggcaagct gcggggcgcg gcggcggacg
      901 ttcaatccgc cgggcagctc gccgtctgcc gccaaaccgc gtttgatgca gccttccatc
      961 acatcggcaa cgccggcaac gcggcggcgg atttcggctt cgccgcatcc ggcaagcgcg
     1021 gcttcgtttg ccaacacgac ttcggagatg tcgagccggt tcagacggca tcgggcaagc
     1081 agttcggcgc aactggtata gggataggga acggcttttt ccgtttccgc ctgccggtca
     1141 aaatcttctt cggtaacgac aaagccgccg ccgaccgaat aataaacctg ttcattcaat
     1201 accgtgccgt ctgaagcata ggcggtaaaa cgcaggctgt tggggtgttt gggcagcact
     1261 tgattgccga gtatgttcag gtcgcggtcg gggatgaagc ggatttcttg cccgttgagc
     1321 cggaggatgt gctgcgtgcg gatgcgttcg aggcgttcgg gaatgccggc aagcgggatg
     1381 tcgtgcggca ggctgccttc caaaccgagc atcagcgcgt caaatgtacc gtgtccgtat
     1441 ccggtcagtg cgagcgagcc gtaaatgtcg atgacgatgc gaacagcctg tgcatccaaa
     1501 cctgccgcaa aggcggcggc tgccttcatc gggccgaccg tatgcgaact ggaaggcccg
     1561 ataccgattt tgaaaatatc gaaaatgctg atcatatttt gctccgacgg tttttcagac
     1621 ggcacaggtt ccgtttgacc aaccaaaaag gagacgcggc acgatgcccg tctccttttt
     1681 taaaacggca cttatgcgtc gatattttgg gcaatcagcg cgttgttttc gataaaggca
     1741 cggcgcggct cgacctcgtc gcccatcagc gtaacgaaca cttcgtcggc ggcaatggca
     1801 tcttcgatgc gcactttcaa caggcggcgc acggcgggat ccatcgtggt ttcccacagc
     1861 tgctcggggt tcatctcgcc caagcctttg tatcgttgga tggacatacc tttttgggca
     1921 acgctcatca agatgtccaa agcggtttca aagctgtccg cgtcgtaccc gttttcgcct
     1981 ttgtaaagct tggcaccctc gccgaccatg cctttgagcg cggcggcggt ttgggtgagg
     2041 gtttggtagg ctttgctgtt gaggaacttg ggttcgatgt agctgaccat gacgttgccg
     2101 tgcagcttgc gcgtgatttt gatgaaccgg tgtccttcat gaccttcgat gcgttcgagg
     2161 gcgacttctt tttcgtcaag cagaccggaa agttcggcaa cggctttatc ggcgttttca
     2221 gacgacgtca aatcaatggg cgacgcgtgt agcatggcgc gcaggacgag ttcgtctacg
     2281 aagcggcttt cctgttcgat gacggttttt gccaacagga attgtttggc ggtgtcggca
     2341 agttctgcgc cttcgatggt gcggccgtct gaaatgattt tggctttttc caaggcaaga
     2401 ccgagcagcc attggtcttt ttccaactcg tccttgaggt aacgttcctg tttgccgtat
     2461 ttcgctttat acaaaggcgg ctgggcgata tagatgtagc cgcgctcgac cagttcgggc
     2521 atttggcggt agaagaaggt caaaagcaag gtgcggatat gcgcgccgtc cacatcggca
     2581 tcggtcatga ta
//
LOCUS       DAWHXZ010000227         2557 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000227 DAWHXZ010000000
VERSION     DAWHXZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.227"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            108..671
                     /gene="gmhB"
                     /locus_tag="WIE35_001876"
     CDS             108..671
                     /gene="gmhB"
                     /locus_tag="WIE35_001876"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ4154753.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENTAPQAD"
     gene            700..1443
                     /locus_tag="WIE35_001877"
     CDS             700..1443
                     /locus_tag="WIE35_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ4154754.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLVRKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPYASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            1440..2132
                     /locus_tag="WIE35_001878"
     CDS             1440..2132
                     /locus_tag="WIE35_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ4154755.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHSRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CRKTTGIRLNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPKAKQW
                     LKIHGRELFALG"
BASE COUNT          611 a          853 c          614 g          479 t
ORIGIN      
        1 accgtactgg tttttgttaa tccactatat cacgcggcac aggcgcatca ctgccgatat
       61 ttcagtataa tgccaccccc gacctgcccc aacccaaagg aaacgcgatg aaactcatca
      121 ttctcgaccg cgacggcgtc atcaatcagg accgcgatga cttcgtcaaa tccgttgacg
      181 agtggatacc tgtcgaaggc agcatggatg cggtggcatt tctgacgcag gcaggctgca
      241 ccgtcgccgt tgccaccaac caatccggca tcgggcgcaa atattttacc gttcaaaacc
      301 ttaccgaaat gcacgccaaa atgcaccgcc tcgtccgtca ggcaggcggc gaaatcaacg
      361 gcatctggtt ctgcccgcac accgatgccg acaactgcaa ctgccgcaag cccaaaccgg
      421 gtatgattga agacatcatc ggacgcttca acgcccaagc ctcggaaacc tggctggtcg
      481 gcgacagcct gcgcgatttg caggcaatcg atgccgtcgg cggcaaaccc gcgctggttc
      541 tgaccggaaa aggcaaaaaa acgctctccc aacacggaca cgaattgccc gaacacacac
      601 aggttttcga taccctgctc gatttctcac aatacatcat gcaggaaaac accgcaccgc
      661 aagccgactg aacataccgc attccgacaa ggcaaaacca tgctcatcat ccgcaacctg
      721 atttactggc tgatactctg ttccaccctg attttcctct ttccctttat gctgctcgcc
      781 tcgcctttcc gagacggggc gcacaagatg gcgcgggtct gggtcaaaat cctcaacctc
      841 tcgctcaaac acatcgtcgg gctcaaatac cgcatcatcg gcgcggaaaa catccccgac
      901 cgccccgccg tcatctgcgc caaacaccaa agcggctggg aaacgctcgc ccttcaggac
      961 atttttccgc cgcaggttta cgttgccaaa cgcgagttgt tcaaaatccc ctttttcggc
     1021 tggggcttga aactggtcaa aaccataggc atagaccgca acaaccgccg cgaagccaac
     1081 gagcagctca taaaacaggg gttggtgcgc aaaaacgaag gctattggat taccattttc
     1141 cccgaaggca cgcgccttgc gcccggaaaa cgcggcaaat acaaactcgg cggcgcgcgc
     1201 atggcgaaaa tgtttgagat ggacatcgtc cccgtcgccc tcaacagcgg cgaattttgg
     1261 ccgaaaaact cctttctgaa atatccgggg gaaatcaccg tcgtcatctg tccgaccatc
     1321 ccgtacgcaa gcggcagcga agccgaattg atgggaaaat gcgaacacct catcgaaacg
     1381 cagcagccgc tcatttccgg cgcaggcccg tttgccgcca aaatgccgtc tgaaaccgca
     1441 tgaccgcctt tgtccacacc ctttcagacg gcatggaact gaccgtcgaa atcaagcgcc
     1501 gtgccaagaa aaacctgatt atccgccccg ccggcacaca taccgtccgc atcagcgtcc
     1561 caccctgctt ctccgtctcc gctctaaacc gctggctgta tgaaaacgaa gccgtcctgc
     1621 ggcaaacact ggcgaaaaca ccgccgccgc aaactgccga aaaccggctg cccgaatcca
     1681 tcctcttcca cagcagacag cttgccctca ccgcccatca agacacgcaa atcctgctga
     1741 tgccgtctga aatccgtgtt cccgaaggcg cacccgaaaa acagcttgcg ctgctgcggg
     1801 actttttgga acggcaggcg cacagttacc tgattccccg cctcgaacgc cacgcccgca
     1861 ccacacaact gttccccgcc tcctcctcgc tgacctctgc caaaaccttc tggggcgtgt
     1921 gccgcaaaac cacaggcata cgcctcaact ggcggctggt cggcgcaccg gaatacgttg
     1981 ccgactatgt ctgcatacac gaactctgcc acctcgccca tcccgaccac agccccgcct
     2041 tttgggaact gacccgccgc ttcgccccct acacgcccaa agcgaaacag tggctcaaaa
     2101 tccacggcag ggaacttttc gccttaggct gacgcggacc ggaccgaccc gccgctttca
     2161 gacggcatcc gtgccggaac aggcacgcgc ccgcccgatt caaaccgcga tgacgctttg
     2221 ccgccggttc ggggcaggat ggcggcacac acgccgtctg ccgcgtttca tttcacacca
     2281 ctcttccgaa acccgaaacc cgcccggtcc gacgtgcggt atgaaacgct taagctgacg
     2341 cgaagtcttt tactgatttg cccgcgaaaa tgccgtctga aaggttttcg gacggcattt
     2401 ttttgcgttt cccaggatgg cggcggattc gtaaaaggcg gtcagggtgg attgtaggat
     2461 gggttgagac ctgccgaatc cgccgcatct gccaaatcta ccgccgtcat tcctacgaaa
     2521 gtgggaatct agaacgcggg gttaagaaaa cctgcat
//
LOCUS       DAWHXZ010000228         2556 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000228 DAWHXZ010000000
VERSION     DAWHXZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.228"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..507
                     /locus_tag="WIE35_001879"
     CDS             <1..507
                     /locus_tag="WIE35_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ4154756.1"
                     /translation="IESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLF
                     EVSRRFNVEIQPQLVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKAL
                     WRNLKNEAPDWAQIIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVV
                     LLLILLLK"
     gene            complement(864..1682)
                     /gene="cysE"
                     /locus_tag="WIE35_001880"
     CDS             complement(864..1682)
                     /gene="cysE"
                     /locus_tag="WIE35_001880"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ4154757.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            1866..2444
                     /gene="grpE"
                     /locus_tag="WIE35_001881"
     CDS             1866..2444
                     /gene="grpE"
                     /locus_tag="WIE35_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ4154758.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
BASE COUNT          591 a          604 c          753 g          608 t
ORIGIN      
        1 atcgaatcgg gctgggtgcc tgccgacacg cgtgcggaag aattggaagc cgccgtccgc
       61 gccgtgtgcg aaccggtgtt caacaaaccg atttcgcaga tttccttcgg cttggtgctg
      121 atgcgcctgt ttgaagtcag ccgccgcttc aatgtcgaaa tccaaccgca gcttgtgctg
      181 ctgcaaaaaa cgctgctcaa catcgaaggc ttgggccgcc agctcgatcc cgatttggac
      241 ttgtggaaaa ccgccaaacc gtttttggtg aaatggatga acgggcaggt cggccctaaa
      301 gccctttggc gcaacctcaa aaacgaagcc cccgactggg cgcaaatcat cccttcattg
      361 ccgcgcaaaa tcagtgcgtt gattgatgaa aaccgccagc aggaaatgcg tgatgcctat
      421 atccatttgg tcaaagtgca gcagcggcaa agcctgtggc tggctgtgat tgcggttgtt
      481 ttgctgctga ttttgctttt gaaataggct ttgtccgaat catcgcccga ctccgcccgt
      541 ttataaggaa atcggttata gtggattaac aaaaaccagt acggcgttgt ctcgccttag
      601 ctcaaagaga acgattctct aaggtgctga agcaccaagt gaatcgattc cgtactatcc
      661 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat atttccggtt
      721 gcgtgggaat cgggtgtatt gaataaaagg cattttgtcc gactggcaag tgccgacatc
      781 ggcggcatat caaggcgcag gcttgaagcg ggcaatgtcg tctgaagccc gtttggcgtt
      841 tcagacggca ttggtgcgga tattcaaatc ataaagtcga tttcggtaaa ctggatattt
      901 tgatccatat ccgccgacgg tgttttgagc gatcgcgcca cgggtttggc gggtacgccg
      961 acaaccgtga tggacggcgg cacgtctgaa accacgacgc tgcccgcccc gattttggca
     1021 ttgctgccga tgcggatatt gcccaatatc gaggcgtttg cgccgatcat cacgccgtcg
     1081 ccgattttag ggtggcggtc gccgccttct ttgcccgaac cgccgagcgt tacgccgtgc
     1141 aaaatcgaaa tattgttgcc caacacggcg gtttcgccgg caacaaagcc ggtggcgtgg
     1201 tcgagcatca gcccgtatcc gaaacgggcg gcgggatgga tgtccacgcc gaatacttcg
     1261 gacatacggt tttgcaggaa atacgccagc gttttgcgcc cgtcgagata cagccgatgg
     1321 ttgatgcggt gtgcctgaat cgcgtggaag cctttgaaat ataaaagcgg cagcgaatat
     1381 tcgtcgcagg cgggatcgcg ttcgtagatg gcttttaagt ctgcttcgac gcatttgccg
     1441 atttgggtgt cgctgcccaa cgcctgctgg tagatttcaa acagcgcgcg cacgtccata
     1501 atcgggctgc cgagtttgct ggaaaggtgg taggcaagga cggagccgag ggactcgtgg
     1561 cgcaacacgg tttggtgcaa aaaacttgcc agcatcggtt cggcggagac cgcggccgcg
     1621 gtttcttcgc ggatggtgtg ccagaggtcg aaaccggttg tgtttaaatg gtcttttttc
     1681 atgagtgatg acgtttgaaa atcgatatgg tcggcagtat cttaccgtct atattatttt
     1741 ttcggtaggg gatttgaaaa tgaatttgaa attctctgct tttgcttgaa gtttcttgaa
     1801 aatgtcctta tcttgcgcgg gtaataactg gattttgatt tccaatttgt tttaagggat
     1861 acgatatgag cgaacagaca cagcagcaaa acagtgaaga agcggttgaa aatgtggagg
     1921 cggtggaaac cgtcgagaca gtaggaaatg cggacggtgt gcaggaacag gctgccgcag
     1981 agccggctta tgaggatttg caggcgcgga ttgccgagct ggaagcgcag ttgaaagacg
     2041 agcagctgcg cgctttggca aacgagcaaa acctgcgccg ccgccaccag caggaaattg
     2101 cggatacgca caagttcgcc ggacagaagt ttgccgtgga aatgctgccg gtcaaggatt
     2161 atctggaaat ggcgcttttg gatcagagcg gcaatttcga tgcgctgaaa atgggcgtgc
     2221 agatgacttt gaacgagttg cagaaagcat ttgatgctac gcaaatcaag gaaatcaacc
     2281 ctaaagcggg cgataagctc gatccgaata tccatcaggc gatgcaggcg gtggcaagcg
     2341 aacaggagcc gaataccgtg gtgggtgtga tgaagaaggg ttatacgctg tccgaccgcg
     2401 tgttgcgccc ggctatggtt acggtggcgc agaaggaagc ctgaaggcgt ctggggaata
     2461 atctgattta tttcctgaag cgcgttttgc gtataaaccg atcgaagtaa agcggcaatg
     2521 ccgtctgaac ccgcctgtcg ggcttcagac ggcatt
//
LOCUS       DAWHXZ010000229         2519 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000229 DAWHXZ010000000
VERSION     DAWHXZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.229"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1..1354)
                     /locus_tag="WIE35_001882"
                     /pseudo
     CDS             complement(1..1354)
                     /locus_tag="WIE35_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
     gene            complement(1369..1623)
                     /locus_tag="WIE35_001883"
     CDS             complement(1369..1623)
                     /locus_tag="WIE35_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154759.1"
                     /translation="MLPIVAYFTAGVKSGGSGMHGAAWRRMRRVCIFVFEIRLLRFEN
                     DIPAATVRNLFAGRARMPSEIEIEAVWRIRYNPRFGFFNI"
     gene            1661..>2519
                     /locus_tag="WIE35_001884"
     CDS             1661..>2519
                     /locus_tag="WIE35_001884"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ4154760.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGIYRHFKAVAEAAAIPMIIYNVPGRTVVSMTNDTILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALK"
BASE COUNT          659 a          872 c          573 g          415 t
ORIGIN      
        1 ctcggtttta tcttcgggca agaccaaatt cagcaagact gccgtaatgc cgccggcgga
       61 aatagagttt tggaacaaga caggcaggtt tttaaacact tccggctcaa acgccacgcc
      121 caagcccaag ccgaccgaag tcgccgcaat caccgcctcg cgcctgcgga tgccgtggct
      181 gaccaaaatc cgcacacccg caatcgcaat caagccgaac atcaaaacca tcgcgccgcc
      241 taaaaccgga ctcggaatcg tcgtaaacgc gcgtccgaca acgggggaac agacccaaca
      301 gcaccaaaat cacggcaata tatttgccca catggcgcga agccacgccg gtcatctgaa
      361 tcacgccgtt gttttgcgca aacgttgtca gcggcagcga acccaaagcc gtcgcaatca
      421 ccgacaccaa gccgtctgcc aacacgccgc cgcgcaggcg tttggtgtat tcctcgcctt
      481 caatcggctg atcggacacc attgccgttg cggtcaaatc gccgaccgcc tcaaacacgc
      541 tcaacaggaa aatcgcaccc gccacaataa atgcgtgcca gtcaaaagca aaaccatatt
      601 taaacggtac gggcagcgta accagcggca ggttttgcag tgccgaaaaa tccaccttgc
      661 ccaaaaacag cgcgacgata tagccggcaa tcagaccgac cgcaatgccg ctcatccgca
      721 gcagcgggtt tttcatgcag ttgaacacca gcacaatcag cagcaccagc gatgccagcc
      781 ccaagttttc catcgagccg aacgtgccgt ccgcctttgc gccgaagccg ccgccgaaat
      841 cggtaatacc gacgtgtacc aaactcaagc cgatcagcat caccaccaca ccgctgaccg
      901 tcggcgtaat cacttttttc aaatacggca gaagccacgc cgagaaacac accaaaaacg
      961 cgccgacaaa cgatacgccc aagagcgtcg aaatcatcgc atccttagtc aaaccgccct
     1021 ctttcatccc cgcgcccagc gcaatcatga cggtaacgaa cgagaaattc accgactgga
     1081 tagacagcat ccccgaaccg accggcccga agcggttgac ctgcaaataa gtgccgacac
     1141 ccgacgcaac catcgccatc gacacgagat acgccgtcat ctccaccggc agttccagcg
     1201 cgccgcccac aatcagcgcg ggcgtaatca tcggcacgaa aatcgccaaa agatgggtaa
     1261 ccgcgctcaa cagcgcatta ccgaacggcg gcctatcctc caaaccgtac accaaatcag
     1321 gcgcatccgc cccttttccc atatgaccgc tcataatgtt tccttgcttc aaatgttgaa
     1381 aaaaccaaag cgcggattgt agcgaatccg ccataccgct tcaatttcga tttcggacgg
     1441 catacgcgcc cgtccggcaa acagattccg aaccgtcgcc gccggaatat cgttttcaaa
     1501 acgcaataac ctgatttcaa acacaaaaat acaaacccga cgcatccgcc gccaagccgc
     1561 cccgtgcata ccggacccgc cggatttcac acctgcggta aagtaggcta caatcggaag
     1621 cattaccgcc ccctacacca caactgataa aaggttctgt atgttacaag gtagcctggt
     1681 tgccctgatt accccgatga atcaagacgg cagcatccat tacgaacaac tccgcgactt
     1741 aatcgactgg cacattgaaa acggcacgga cggcatcgtc gccgtcggca cgacaggcga
     1801 atccgccacc ctctccgtcg aagaacacac cgccgttatc gaagccgtcg tcaaacacgt
     1861 tgccaaacgc gtccccgtca tcgccggcac aggggcgaac aacaccgtcg aagccatcgc
     1921 cctttcccaa gccgccgaaa aagccggcgc ggactacacc ttatccgtcg tcccctatta
     1981 caataagccc tcccaagaag gcatttaccg ccatttcaaa gccgttgccg aagccgccgc
     2041 gattccgatg attatctaca acgtgcccgg ccgtaccgtc gtcagcatga ctaacgacac
     2101 tatcctgcgc cttgccgaaa tccccaacat cgtcggcgtg aaagaagcca gcggcaacat
     2161 cggcagcaac atcgaactca tcaaccgcgc gcccgaaggc ttcgtcgtcc tgtccggcga
     2221 cgaccacacc gccctaccgt tcatgctctg cggcggacac ggcgttatta ccgtagccgc
     2281 caacgccgcg cccaagctct ttgccgatat gtgccgcgcc gcgcttcaag gcgatatcgc
     2341 ccttgcacgc gaactcaacg accggctgat tccgatttac gacaccatgt tctgcgaacc
     2401 cagccccgcc gcaccgaaat gggcggtatc cgccctgggc agatgcgaac cgcacgtccg
     2461 cctgccgctc gttccgctga cggaaggcgg acaggcaaaa gtccgcgccg ccctgaaag
//
LOCUS       DAWHXZ010000230         2518 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000230 DAWHXZ010000000
VERSION     DAWHXZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.230"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            53..532
                     /locus_tag="WIE35_001885"
     CDS             53..532
                     /locus_tag="WIE35_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ4154761.1"
                     /translation="MPSETAIADITRTYSAHYLNPNNRNMSLFPQALPCLDKLKAQGY
                     WLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGICGKLGLDPKEA
                     LVVGDTAHDLHMAANAGAAAVGVATGAHSREQLRTAPHLAVLDGLSELPGFLAQHYA"
     gene            complement(619..1587)
                     /gene="ispH"
                     /locus_tag="WIE35_001886"
                     /gene_synonym="lytB"
     CDS             complement(619..1587)
                     /gene="ispH"
                     /locus_tag="WIE35_001886"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ4154762.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAAGIGTDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1616..2113)
                     /locus_tag="WIE35_001887"
     CDS             complement(1616..2113)
                     /locus_tag="WIE35_001887"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ4154763.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
BASE COUNT          577 a          772 c          617 g          552 t
ORIGIN      
        1 ggaattttgc aaaggtctca gcctgcccga aatcatccgc gacctgctcg aaatgccgtc
       61 tgaaaccgcc attgccgaca tcacacgcac ttattccgca cattacctca atcccaacaa
      121 ccgcaatatg tccttatttc ctcaggcact gccctgtctg gacaagctca aagcacaagg
      181 atactggctt gccgtcgcca cgggcaaagg gcgggcgggt ttggacaacg ccatcagcca
      241 aaccgccacc ggcggctatt ggctcgccac cgcctgcgcg ggggaatatc cctccaaacc
      301 ctcgcccgaa atggtattcg gaatctgcgg caaactggga cttgacccga aagaggcatt
      361 ggtcgtcggc gatacggcgc acgacctgca tatggcggca aacgcaggcg cggcggcagt
      421 cggcgtggcc accggcgcac attcgcgcga acagctccgc accgcaccgc atctcgccgt
      481 attggacggt ttgtccgaac tgcccggttt tcttgcacag cattacgcct gattggtttc
      541 cgcatcagac actcaaaaca aatgccgtct gaagcctgtt cagacggcat ttgtgttgcc
      601 caaacattca acgcttgctc aacgtttgca caaatcgggt ttggtttcgc cctcgcggcg
      661 cagctctttg ggcaggacga acacaatgct ttcttccgca ccctcgcctt cgcgtacggt
      721 ttcgtgcccc catccgcgta tggttgccag tacttcccgc accaacactt cgggcgcgga
      781 cgcgcctgcc gttacgccga ctttgttctt gccctcaaac cacgcgcgtt gcaggtagcc
      841 tgcattgtcc accatatacg catcggttcc gatgcctgcc gccacttcgc gcaggcggtt
      901 gctgttggac gaattgggcg aaccgaccac aatcacgatg tcgcactgtt ctgccaactc
      961 tttgacggcg gtttgccggt tggtcgtcgc atagcagata tcttccttgt gcggattgcg
     1021 gatattgggg aaacgcgcgt tcagcgcggc gatgatgtct ttggtttcat cgaccgagag
     1081 cgtggtttgg ctgacatagg cgagtttgtc ggggtttctg acttcgagtt ttgccacatc
     1141 tccgaccgtt tcgaccaaaa gcattttgcc cggcgcaagc tgccccatcg ttccttcaac
     1201 ctcgacgtgc cccttatgcc cgatcatgat gatttcacag tcttgggcat ccagtcgggc
     1261 gacttcctta tgcactttcg tcaccagcgg gcaagtcgca tcaaatacgc ggaaaccgcg
     1321 ctccgccgct tcttgccgca ccgccttcga tacgccgtgt gccgaataaa ccagtgtcgc
     1381 gcccggcggc acttccgcca agtcttcaat aaacaccgca cctttttcac gcaggttgtc
     1441 cacgacgaat ttgttgtgaa cgacttcgtg gcgcacataa atcggcgcgc cgaattcttc
     1501 caaagcacgt tcgacaatac tgattgcccg atccacgccg gcgcagaagc cgcgcggatt
     1561 ggcaagaatg atggttttct cgttcataag cccggtattt cgttttcaga cggcatcaat
     1621 atttttcttc ttgggtttta cggtggacga tgttgtccaa cactgccaac accgcaccga
     1681 cgcagataaa gctgtcggca atattaaagg cgggataaaa ccaattttgc caataaaaca
     1741 ataagaaatc gacgacatga ccgtgtatca ggcggtcgat gacattgcct aacgcaccgc
     1801 cgataatcat cgccgcaccc gttttgccga gggttgcaaa ctcatcgcgc aagatggcgc
     1861 gtaccaaata cgcgctcacc gccaccgcca gcaccaaaaa aaagtatttt tgccagccgc
     1921 cctgatcggc aaggaagctg aacgccgcac ccgggttgta caccaaggtc aaatcgaaaa
     1981 aggaaggaat gacattgacg cgttcccgat actgaaacga cgacagcacc gcccacttcg
     2041 accactggtc cagcacgatg gcggcaagtg ccaataccca atagcgcgtt ttacttgaaa
     2101 cagatgaaga catatttttc aacagccggt aaaagagtac cattttaccc gaaaaccccc
     2161 tgcctgtacc cgaaacggca aatgccgtaa tcttgaaacc cgtcattccc gcgcaggcgg
     2221 gaatctagac ctgtccgcac agaaacttat cggataaaaa cagttgctca aaccccgcgt
     2281 tctagattcc cactttcgtg ggaatgacgg ttcagttgcg ttccaacaac accgcaatct
     2341 cgaaatccgt cattcccgcg caggcgggaa tccagacctc cgacgcggcg ggaatctatc
     2401 ggaaatgact gaaaccccga gattctagat tcccactttc gtgggaatga cggttcagtt
     2461 gcgttccgac aacaccgtaa tctcgaaatc cgtcattccc gcacaggcgg gaatccag
//
LOCUS       DAWHXZ010000231         2502 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.231, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000231 DAWHXZ010000000
VERSION     DAWHXZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.231"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..329
                     /locus_tag="WIE35_001888"
     CDS             <1..329
                     /locus_tag="WIE35_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ4154764.1"
                     /translation="IATTVPAQQTFLDAAELMQWSIETEWLGLNVIAHCNFGKQHAQV
                     EFEACFQDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQPCICGSGKKFKACCGK
                     YLKPVA"
     gene            complement(548..1342)
                     /locus_tag="WIE35_001889"
     CDS             complement(548..1342)
                     /locus_tag="WIE35_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ4154765.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVEWIRENQ"
     gene            1386..1490
                     /locus_tag="WIE35_001890"
                     /pseudo
     CDS             1386..1490
                     /locus_tag="WIE35_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1551..2276
                     /locus_tag="WIE35_001891"
     CDS             1551..2276
                     /locus_tag="WIE35_001891"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ4154766.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          681 a          776 c          557 g          488 t
ORIGIN      
        1 tcatcgctac taccgttccg gcgcaacaaa cattcctcga tgccgccgaa ctgatgcaat
       61 ggagtataga aaccgaatgg ctgggcttga atgtcatcgc acactgcaac ttcggcaaac
      121 agcacgccca agtcgaattt gaagcctgct tccaagacgg acaacaccga tccgcacatc
      181 acgaactgtc cggcttcgtc aacatcggcg gacaatggta ttttatcgat cccaccgttc
      241 cgcatcctgc gatgaaacaa ccctgcattt gcggatcggg caaaaaattc aaagcctgct
      301 gcggcaaata tctgaaacct gtcgcataaa aatgccgtct gaacgttcag acggcatttt
      361 caacgtgcaa aaaaaaacca ttcataccaa gggtaagtat gaatggtcaa tacattgcgg
      421 gaaaacgtct tacttgctgc actgccgaaa agggagaaac ggcagcggta atcagcggaa
      481 aggattgtac ccgaattaat gttaagaaac gttaatcgtg aaaatatatt aacaaacctg
      541 ttgaaaccta ttggttttcc cgtatccact cgacccagcg ttcaaacagc ttcggttcga
      601 gcgcggcgac gaccgagcgt ttgaacacgt gttcaccact ccaaaacccg tcgccttcca
      661 aagtcgtcag cctgccgccc gcctcctcga aaatcaacgc gccggcggca taatcccaca
      721 gcttctgccc gccgtggaca taaacatcat aacgcccgca cgccagataa caccagtcca
      781 acgtactgct gcccatactc cgtatcgttc caaaaggcgc gagcgtactc atgcggctgg
      841 acagtttgcc cgaacgcaga tatttgattt ccacgcccgc gattgcctca ttcaacttct
      901 tatccacgag acgcaaaggc aggcgtgtcc cgtttaaaaa cgcaccctgc ccgcgttcag
      961 cataaaaaca ttcgccgctg accggattgt agattacgcc caattcggcg cgcccgttgc
     1021 ggacaaacgc caccgacacg gcaaaatgcg gcagcccgtt gacaaaattg tttgtcccgt
     1081 ctatcggatc gacaatccac agcccctttt cccccgaata ttgttcccac agagccgact
     1141 gttcctgtcg cgacatttcc tcgcccaaca tcgggcaatc gatcagaagc ggcaacgcgg
     1201 cggcaaaagc cgtctgcgcg gcaatgtccg cctcgctcaa catcgaaccg tcttccttgc
     1261 ggtgggacgg cgtattcaaa aaacgcggca taatttcggt ttgcgcgata tggcgcacga
     1321 ctttctgcaa acggtgtaac acttcctact gtcctcatat tttgaacttg cggcacgcga
     1381 acgtatatag tggattaaat ttaaatcagg acaaggcgac gaagccgcag acagtacaaa
     1441 tagtacggca aggcgaggca acgctgtact ggtttaaatt taatccacta taatgtccgt
     1501 ttccatcacg ccgctgcgac agattataac cgtccgaacc gccaaaaact atgccccgat
     1561 tccacctgcc cgaaaacctt tccgtcggac aaaccgtcgc cctgcccgac aacatcgtcc
     1621 gccacctcaa cgtcctgcgc gtccgcccca acgaaaacat caccctcttc gacggcaaag
     1681 gcaaggcaca cgccgcacgg ctgaccgttt tggaaaaacg ccgcgccgaa gccgaaatcc
     1741 tgcacgaaga cacaaccgac aacgagtccc cgctcaacat cacactgata caatccatct
     1801 cctccggcga tcgcatggat ttcaccctgc aaaaaagcgt cgaactcggc gtaaccgcca
     1861 tacagcccgt catcagcgaa cgctgcatcg tccgcctcga tggggaacgc gccgccaaac
     1921 gcctcgcacg ctggcaggaa atcgtcatct ccgcgtgcga acaaagcggc aggaacaccg
     1981 ttccccccgt gctgcccatc atcggctatc gtgaagcact cgacaaaatg ccgtctgaaa
     2041 gcaccaagct gattatgagc atcaaccgcg cccgcaaact cggcgacatc cgccacccgt
     2101 ccggcgcaat cgtctttatg gtcgggcccg aaggcggctg gacagaacag gaagaacaac
     2161 aggcatttga agctggcttt caggcggtta cactcggcaa acggatttta cgcacagaaa
     2221 ccgccccact cgccgccctc gccgccatgc agacgctttg gggcgatttc gcataaacag
     2281 aaatgccgtc tgaaacccgt tcagacggca ttttgcagcc gattaagata gtaggttcaa
     2341 ataagatttc ccgtgtcgtc attcccgcga aagcgggaat ctagaaacga aaaactacag
     2401 ggatttatcc gaaacaacaa accctctccg ccatcattcc cgcaaaagcg ggaatctaga
     2461 aacgaaaaac tacagggatt tatccgaaac aacaaaccct ct
//
LOCUS       DAWHXZ010000232         2474 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.232, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000232 DAWHXZ010000000
VERSION     DAWHXZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.232"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(43..951)
                     /locus_tag="WIE35_001892"
     CDS             complement(43..951)
                     /locus_tag="WIE35_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ4154767.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLQHLSAAVSGGYYYAPSVERLQNL"
     gene            1236..2063
                     /locus_tag="WIE35_001893"
     CDS             1236..2063
                     /locus_tag="WIE35_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4154768.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            2053..>2474
                     /locus_tag="WIE35_001894"
     CDS             2053..>2474
                     /locus_tag="WIE35_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ4154769.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAG"
BASE COUNT          527 a          628 c          723 g          596 t
ORIGIN      
        1 aaaatgccgt ctgaaaaatg gtttcagacg gcatcgtccg gcttagaggt tttgcaggcg
       61 ttcgacagac ggcgcgtagt agtatccgcc cgaaacggcg gcggagagat gttggagcag
      121 caggtcggtt ttgccgtccg tatcgccgaa catactcaat aattgcgctt cgatattgtg
      181 cagcgtgcgg cagtatgcgg taaacatcaa accgtgttcg ccgctgattt tgccgaaggg
      241 caggctgcgg cggacgattt tcaggccgac tccgttttct ttcaggttga cgcggccgag
      301 gtgcgaatcg ggcaggcgga catcgcggcc gaattcgtcg tcggtttcct tgccgcgtcc
      361 gaccgaggcc tcctgttcgg cgacggggac ggcatcccat tttttcaaat cgtgcaggta
      421 tttttgcagc aggacatagc tgccgcccgc atcgggtttt ccttcgggga tgatggcgac
      481 ttcgcggaca ttttcatcgc cctgcgggtt ttccgtgccg tcgacgaaac cgtccagccc
      541 gcgatcctga tacaggcgca aaccgtgttc ttcggacgcg acgcatatgc tgtcgccgaa
      601 cgcgcccaaa acggattggg caagcgcgta ggcggcgttt tggcggaaag attggatgtg
      661 gatggacata tcgtgctgcg tggacggcgc aagcccgttg cccatttcgg agaagggttt
      721 gatttcactg ccttcgtccg tatgtccgaa tgttgcccag gctttgctgc cgaaggcgat
      781 ggtcagaccc aaaatatcgt ccggaaagcg ggttttcaag gcggataacg cgtcgagcga
      841 agcgcggcag gcggctttaa tatcgttgag gcggttggcg gcgaagtcgg cttcgataaa
      901 gatgccggct tgggcgtggt cgggaatgat ggcggattgg ggcgtgttca tgagatgttc
      961 ctttttggtg tcatctgttt cggatggatt atagcaccga atcggcaggc ggatttttgc
     1021 cggaacggcg tgcgtgaatc cgccgtttgc atacctgatg ccgcttttcg gtttcgtgcc
     1081 gcccgtcgcc tttcccgccc cctttatttc cgcttccggc ggcttcggca tatcttttcc
     1141 attccgattt ggaataacca tataaaaaaa gtattctttg tgtttgccgc aatttcactt
     1201 agaatgccgc acttgcacac tttttacagg agagaatgat gttgaaaaaa ttcgtactcg
     1261 gcggcattgc cgcattggtt ttggcggcct gcggcggttc ggaaagcggc agcggagcat
     1321 cttccgcgcc tgcacaatcg gcagtttccg gttctttaat cgagcgcatc aacaataaag
     1381 gcacggttac cgtcggcacg gaaggcactt acgcaccgtt tacctaccac gacaaggacg
     1441 gcaaactgac cggttacgat gtggaagtaa cccgcgccgt ggcggaaaaa ctgggcgtga
     1501 aagtcgagtt taaagaaacg caatgggatt cgatgatggc gggtttgaag gcggggcgtt
     1561 tcgacgtggt ggcaaaccaa gtcggtctga ccagccccga acgccaagcg acattcgaca
     1621 aatccgatcc ttacagctgg agcggtgccg tattggttgc ccgtaacgac agcaacatca
     1681 aatctatcga cgacatcaaa ggcgtgaaaa ccgcacaatc cctgaccagc aactacggcg
     1741 aaaaagccaa agctgcaggc gcagatttgg tggcagtgga cggtttggcg caatcgctga
     1801 ccctgattga acaaaaacgt gccgatgcaa ccctgaacga cgaattggcg gttttggact
     1861 atctgaagaa aaacccgaat gcgggcgtga aaatcgtttg gtccgcacct gccgatgaaa
     1921 aagtcggttc cggcctgatt gtcaacaagg gcaatgacga agccgtggcg aaattcagta
     1981 cggcaatcaa cgagctgaaa gccgacggta cgctgaaaaa actgggcgaa caattcttcg
     2041 gaaaagacat cagtgttcaa taatttcctt gcttcgctgc cgtttatgac ggaaacacgc
     2101 gccgatatga ttgtcagcgc gtttttgcct atggtcaaag ccggcttcgc ggtctctctg
     2161 cctttggcgg cagcttcttt cgttatcggt atgatgattg cggtagccgt ggctttggtg
     2221 cggattatgc ccgccggcgg catcgtgcgg aaaatcctgc tgaaattggt ggaattttat
     2281 atttccgtca ttcgcggtac gccgctgttg gttcagcttg tgattgtgtt ttacgggctg
     2341 ccttccgtcg gcatctatat cgacccgatt cctgccgcca tcatcggctt ttcgctcaat
     2401 gtcggcgcat atgcttccga aaccatacgc gcggcaattt tgtccgtacc gaaaggccaa
     2461 tgggaagcag gttt
//
LOCUS       DAWHXZ010000233         2458 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.233, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000233 DAWHXZ010000000
VERSION     DAWHXZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.233"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            35..1390
                     /locus_tag="WIE35_001895"
     CDS             35..1390
                     /locus_tag="WIE35_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ4154770.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="WIE35_001896"
     CDS             1394..2404
                     /locus_tag="WIE35_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ4154771.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     TYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          453 a          664 c          740 g          601 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggtt
      781 cgaactctta aaaaccgcgc ctaaagcgtt ttggacggtt actctggtac agtttttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta tcaggaggcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtaacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgccgcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttccgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat ttcggcctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgac gatgcggctt cctatgcgga caatccgttt cagattaaca agcagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttacctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaacggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgaat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ctagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgcagacg gcctttttat agtggattaa caaaaacc
//
LOCUS       DAWHXZ010000234         2452 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.234, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000234 DAWHXZ010000000
VERSION     DAWHXZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.234"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(515..>2452)
                     /locus_tag="WIE35_001897"
     CDS             complement(515..>2452)
                     /locus_tag="WIE35_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ4154772.1"
                     /translation="ISPEIFGTLFQSVMDAQERREAGAHYTETANIDKVINGLFLENL
                     RAEFEAVKALKRDKDKKLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAE
                     ALKDKTGGLFDSPSVQCRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDGEV
                     ACHTLPLEDAAEIIHANSLRTPWQAADYIFGNPPFIGSTYQTKEQKNDLESICGHIKG
                     YGLLDYVCNWYVKAAGIMAQHPQVQTAFVSTNSICQGQQVEILWGSLLKQDIEIHFAH
                     RTFQWTSQAAGKAAVHCIIVGFRQKPQMPSEKTLYDYPDIKGEPVKHAAANINPYLID
                     APDVIIAKRSRPVHCEPDMVNGSKPTEGGNLILSTDEKEALIAAEPLAEQYIRPFVGA
                     EEFINGKTRWCLWFHGVSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATP
                     WLFQKIRQPSDGNFLIIPRVSSESRRFVPIGYLSYETVVSDLVFTLPNASLYHFGILS
                     STMHNAFMRTVAGRLESRYRYSNTVVYNNFPFPESCRMPSENDRQDPLRAAVETAAQA
                     VLDARGQYRREAREAGLPEPTLAELYAPDAGYTALDKAHAALDKAVDKAYGYKTGKNT
                     DDEAERTAFLFELYRKAAAIA"
BASE COUNT          480 a          544 c          801 g          627 t
ORIGIN      
        1 aaacgcccga aaaaaccgaa atccgacagg caggattttc gctttcgtgg ggatgacggg
       61 atttttgtaa ccgtagcaac tgcttttgcg gtaatgacgg gattttaagt tacggtaagt
      121 tatcggaaag acagaaactt ctccgccgtc attcccgcga aggcgggaat ctgaacacgt
      181 ccgtagggaa acctatatcc cgtcattccc atgaaagtgg gaatccagga tgcaggggaa
      241 atcgttttat ccgataagtt tccgccccga aaggtctgga ttcccgcctg cgcgggaatg
      301 acgggatttt tgtaaccgca gggtgggctt cagcctgcca atcaacaacc gaaaatgaag
      361 aaaaacggtg ggctgaagcc caccctacgc aattgctttt gcggagatga cggggtttta
      421 gggtgaagat ggtgggagtt ggtgggagtt ggtgggctga agcccaccct acgcaactat
      481 cctacgcaac tactacgcaa ctactacgca actactacgc aattgccgcc gccttgcggt
      541 acagctcgaa caggaaggcg gtgcgttcgg cttcgtcgtc ggtgtttttg cctgttttgt
      601 agccgtaggc tttatcgact gccttgtcga gggcggcgtg ggctttgtct agggcggtat
      661 agcctgcgtc gggcgcatag agttcggcga gggtcggctc gggcaaaccg gcttcccgcg
      721 cttctcggcg gtattgtccg cgcgcgtcga ggacggcttg ggcggcggtt tcgacggcgg
      781 cgcggagcgg gtcttggcgg tcgttttcag acggcatccg gcagctttcg gggaagggga
      841 agttgttgta cacaacggta ttggaatagc ggtaacgact ttccagacgg ccggcaacgg
      901 tacgcataaa ggcgttgtgc atggtggagc tgaggatgcc gaagtggtag aggctggcgt
      961 ttggaagggt aaataccaaa tcgctgacaa ctgtttcata tgatagataa ccgatgggga
     1021 cgaaacggcg gctttcggat gagacgcgtg ggataatcag aaaattgccg tctgaaggct
     1081 ggcggatttt ttgaaaaagc cacggggttg ctgcatcttt ttgagtttgt ttgtcgctgc
     1141 tggcttcgcg catggttttg actgcctgaa tacgggcttg aacttggggc atctgtttca
     1201 ggtcgtggtt gcgtttaacg tcggatacgc cgtgaaacca caggcaccaa cgggttttgc
     1261 cgttgataaa ttcctctgcg ccgacaaagg gacggatgta ttgttccgcc aagggttcgg
     1321 cggcaatcag ggcttctttt tcatcggttg aaaggataag gttgccgcct tcggtcggtt
     1381 tgcttccgtt gaccatgtca ggttcgcaat gtacgggacg gctgcgcttg gcaataatca
     1441 catcgggcgc gtcaatcaga tacggattga tgttggccgc ggcgtgtttt acgggttcgc
     1501 ctttgatgtc gggatagtcg tagagggttt tttcagacgg catttgcggc ttttggcgga
     1561 agccgacgat gatgcagtgg acggcggctt tgcctgcggc ttggctcgtc cattgaaagg
     1621 tgcggtgggc aaagtggatt tcgatgtctt gcttcaaaag gctgccccaa aggatttcga
     1681 cctgctggcc ttggcagatg gaattggtgg aaacaaatgc cgtctgaact tggggatgct
     1741 gcgccatgat accggcggct ttgacatacc agttgcagac gtaatccaac aggccgtagc
     1801 ctttgatatg gccgcagatg ctttcgaggt cgtttttctg ctctttggtt tggtaggtcg
     1861 agccgataaa gggcggattg ccgaagatgt agtccgccgc ctgccaaggt gtgcggaggc
     1921 tgttggcgtg gatgatttcg gcggcgtctt cgagcggcag cgtatggcag gcgacttcgc
     1981 cgtcgaagcg gatttgcgtg cggatgttgc attggtggtt tttgagccac atggcggtgc
     2041 gggcgatgag gacggcaaat tcgtctattt cgatgccgtg aaactgtttc agacggcatt
     2101 ggacggacgg gctgtcgaat aagccgcctg ttttgtcttt gagcgcttcg gcgatgatgt
     2161 cgtcttcgag ggcgcggata cggtcgtagg cgacgataag gaagttgccg cagccgcagg
     2221 ccgggtcgag gaattgcagg ttttggattt tttggtagaa ggcggcgagt tttttgtctt
     2281 tgtcgcgttt gagggctttg acggcttcaa attcggcgcg caggttttct aaaaaaaggc
     2341 cgttgatgac tttgtcgatg ttggcggttt cggtgtagtg tgcgcccgct tcgcgccgct
     2401 cttgtgcgtc catgactgat tggaagagcg tgccgaagat ttcgggactg at
//
LOCUS       DAWHXZ010000235         2439 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.235, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000235 DAWHXZ010000000
VERSION     DAWHXZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.235"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(135..1586)
                     /locus_tag="WIE35_001898"
     CDS             complement(135..1586)
                     /locus_tag="WIE35_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ4154773.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(1609..2229)
                     /locus_tag="WIE35_001899"
     CDS             complement(1609..2229)
                     /locus_tag="WIE35_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ4154774.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGILYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
BASE COUNT          765 a          580 c          568 g          526 t
ORIGIN      
        1 taatccacta tatataggaa taagaattcg ggacaaaatg tttaaaccat tttgtcccga
       61 ctgctgtgat gcggtttttt tgaactagca ggacgttacc taaaacctgc gttgcctgaa
      121 accctttaag acggttaaat ccctctgccg tatttgtatt ctacaatcat attatcgtcg
      181 gtaatgactt ccgccccagc cgaaggttcc gtcatctgaa tcagcatacg agagacaacc
      241 ttttgtgctg cagcatccac ggtgctgctg tcaaatacgt gcctgccgct ttccggccaa
      301 atcaaccggg agagacgttg cttgagcagt tctttattag ggaaaactac cggggttgcc
      361 gagccgacta ccatatgccc atagcggtat gcatagggaa tactgtgtac ggcggtagca
      421 aaagcatgcg ggctgtgcgt ggtattaaac attacaatac catccggggt aaggtggctt
      481 tgcacctgtt ttaaaaattc cgcactcaac aggttggtgg aataggcacg ccagtaccaa
      541 gtcgtattca tcaaaatcag gtcgaatttt tcatcaggat ggcgacgcag ccatttccta
      601 ccgtcatcca atacaatttc aacacgtttg tcctgcaaaa gcggggcgat ttgcggctcg
      661 tctgcgataa ggctacggta tgccggattg atttccgcaa cgatcatcga ctgcatttcc
      721 ggaatggcag acaagacgcg cgcccacgaa cctgtactca atccaacgac gaaaatgcgg
      781 cgtatgccag acttcaggga gggtagcaga taggcacgtt cgatgccgtt gacactattg
      841 aatacatcgg tattgtatgc gccgtcgtat acattcgccc cataaacaac cttatcacca
      901 tctctatggt aaaccgcaac aatgccgtgt ttgttttcaa tcagcctatc cggacggtca
      961 gcaatatttt gaaagacaga atccggcagt aggaacatga ggatgccgaa cattagggaa
     1021 actgctaccg acactgcatt cagtcggaga cttttttgga acagtgtaca aaacaaaggg
     1081 acagcagcag aaatcaaaca gatgagcagg taaatctgtt gggtggacaa gaaatcaagt
     1141 atcacaaagc cgataaggac cggacccaat gcactgccgg caacgttggc gaaataaaca
     1201 ttggaaacct gtcgtccgga tttgttgcca tccgtaccca catggtgtac gagcgggaaa
     1261 atcaaccctc tgacgacggc agacagggta atgaagatac cggcgtggtg gacgaagccg
     1321 gaaaaacccg tcaacaacca cgcagcaccc aaaatcaaaa agtcggcaat acccgcccac
     1381 aagaagcact gcccgataaa gggaatatca acaaagcggc tgcggcaaat ccgtttgcca
     1441 aaatacgcgc cgacggcgat accggtcaga aaacaggcaa gagtaaatga aaatgcctga
     1501 ggcacggact gtgctgcgaa cgaaaacatc cttacccaca agacttctat acccaagctc
     1561 aataagccgc taaggaaaga aagcatataa atcaaactag tattcggttt agtgttcact
     1621 atatccatcc tttctgtaac gcagccatac tgaagcagca atcagaaggt taaagcaggc
     1681 tgtcagcgca atggtttggg agagggtaaa aaagacgtag aaaaattcgg cggcggcaag
     1741 cgatccgagt gccgcaccca aagtgttgaa aaaatataag ataccgatag actcgccaac
     1801 attatgtatt ttccggttaa aaaaacaggt cagcaagggc aaggtcgcgc ccatcataaa
     1861 ggtaggaagc agcaataaga ggaaattggc agcagcgatg atgggcaaat cagcctcaac
     1921 taaaagatgc cccaagccgg aaatcagacc cctgcttacc aaaccgaaca gaccgatgga
     1981 tacttcagcg atgcaaaaca gggggatgat acttgaagga aaacggtcag caatgcgtcc
     2041 accgaaatac gcacccacac ccaagccgac cataaataca gaaataatga cagtaatcga
     2101 acttaaatcg atacctatgt gactgaatag aagcctctgc cagctgacct ggtaaatcag
     2161 ggcgcagaat ccagaggcga aaaacaccag cgacaatcct ttcagggttt tacttgcggt
     2221 cgaattcata ttttctattt tcaaataggc ggaactataa atgaaatgta tcaatgtgta
     2281 aacatttgta ttaaccgtag gaaatcaccg tattgctcaa actgcaggcc gggaaaataa
     2341 actcgtaagc ccgcaaacgg cgtatcactg ttcaaactgc tgtgcggctt cacggtgtta
     2401 taaaaataat ggggctgtcc tggacaacta agataaact
//
LOCUS       DAWHXZ010000236         2436 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.236, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000236 DAWHXZ010000000
VERSION     DAWHXZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.236"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            662..1174
                     /locus_tag="WIE35_001900"
     CDS             662..1174
                     /locus_tag="WIE35_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ4154775.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAVAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            1254..2333
                     /gene="aroB"
                     /locus_tag="WIE35_001901"
     CDS             1254..2333
                     /gene="aroB"
                     /locus_tag="WIE35_001901"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ4154776.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          599 a          744 c          601 g          492 t
ORIGIN      
        1 aacccaaaac ggcaacttaa aaccccgtca ttcccgcgca ggcgggaatc taggtctgtc
       61 ggttcaggac cttatcggat aaaacggttt ctccaaccct gcgttctaga ttcccacttt
      121 cgtgggaatg acgggatatg ggtttccgtg cggacgtgtt cggatttccg cctgcgcggg
      181 aatgacggcg acagatgccc aacggtcttt atagtggatt aacaaaaatc aggacaaggc
      241 gacgaagccg cagacagtac aaatagtacg gaaccgattc acttggtgtt tcagcacctt
      301 agagaatcgt tctctttgag ctaaggcgag gcaacgctgt actggttttt gttaatccac
      361 tatagtattg ataaacatat tatcttcaat atattcaatt ggataattgt ttacctaagc
      421 aaagataatt gccttttcct gacaaataag tgaaatcaac ggattgtcaa aacacagcct
      481 gaaataaaaa acctccctga tttcttttat ttttccttaa aatcagaaag gttcgggatg
      541 gtcgggttat ttttccaaac gtaccgctgc cctgccgatt tcgtataaaa ttccgccgta
      601 acccgacaag cccgaaccct gtcgccccga aaggcggggt gtcaaacatt aaggaattgt
      661 gatgaaaaac tttaacggca aactcatcct catcggactg atgggcgcgg gcaaaaccac
      721 gctgggccgg caaatggcgc agcggctgga ttaccgtttt tacgacagcg atcacgaaat
      781 cgccgtagcc gccggcgttc ccatccccac catatttgaa atggaaggcg aacagggatt
      841 ccgttcgcgc gaaaccgcca tactcaaaaa gctggttatc ctgccccata tcgtcctgtc
      901 caccggcggc ggcgcggtgt taaaagaaga aaaccgcgcc cttatccgca aaagcggcac
      961 ggtcgtctat ctgcacgccc cgcccgaaac cctgctcgaa cgcacgcgct gcgacaacag
     1021 ccgtcctttg ctgcaagttg ccgatccttt ggcgaaatta cgtgaactct acgccgcacg
     1081 cgaccccgtt taccgccaaa ccgccgactt taccgtagaa tcggcaaact gccgggaaac
     1141 cgtgcaaacc ctgctcaaac gcttatcccg ataaaccggc atatgcgccg cgcccagaaa
     1201 accaagccgc gccccgcccg gcgggccggc ggttcaaact ttaaggaaca acaatgaaaa
     1261 cactgaccgt acacaccccg tcccacagct accccatctt tatcggcaac gggctgctgc
     1321 cgcaggcagg aagcctgctc aaaccgcatt tgggcaaacg cgccgccatc atcgccaacg
     1381 aaaccgtcgc cccgctctac ctcggcacgc ttcagacggc attggatgcg gcaggcgtat
     1441 cccatttcag catcatcctg cccgacggcg aggcgcacaa aaactggcag acgctcaacc
     1501 tcatctttga cgggctgatg caaaaccgcg ccgaacgcaa aaccacatta atcgcactgg
     1561 gcggcggcgt gatcggcgac atggtcggct ttgccgctgc cacctaccag cgcggcgcac
     1621 cgttcgttca aataccgacc acactgttga gtcaagtcga ctcatcggtg ggcggcaaaa
     1681 ccgccatcaa ccacccgctc ggcaaaaata tgatcggcgc attctaccag ccgcaggcgg
     1741 tgcttgccga cttggacacg ctgcacaccc tgcccgtccg cgaattgtcc gccggtatgg
     1801 cggaagtcat caaatacggc gcgctcggcg acatcggttt tttcgaatgg ctcgaacaaa
     1861 acatatccgg actgatgacg ctcgatcggg aaaaactcgc ccaagccgtg taccgctgct
     1921 gccaaatgaa ggcagacatc gtcgcccaag acgaaaccga acagggcata cgcgcatggc
     1981 tcaacctcgg acacaccttc ggacacgcca ttgaaaccga gatgggttac ggcacttggc
     2041 tgcatggaga agccatcgcc gccggctgcg tgttggcggc gcgtttgtcc gaacaactgg
     2101 gcaaaacctc cgccgcagat accgcgcggc tcgccgccct gctcgaagcc gccggactgc
     2161 cgtccgcacc gcccgtgttt gcctttgaaa aatggctcga acatatgagc cacgataaaa
     2221 aagtcagcgg cggcatcatg cgctttatcg gtctgaaccg gctgggcgaa gccaacatca
     2281 ccgaaattac cgacacggac atcctccgcc gcaccctgca accgtatctc tgatttcctc
     2341 tgccgatgtg ctgccgcgcg ggtttgacgc acgatgatga ttttccatca tctttctccg
     2401 taaaagcggg aatccggtcc gttcggtttc ggtcgt
//
LOCUS       DAWHXZ010000237         2419 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.237, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000237 DAWHXZ010000000
VERSION     DAWHXZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.237"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            9..665
                     /locus_tag="WIE35_001902"
     CDS             9..665
                     /locus_tag="WIE35_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEZ4154777.1"
                     /translation="MILHPSSLNYHVIPKGADFSDNDFVRHFETLVEGRYYIANAWTK
                     IVRREIIIKNNLFFPKGYIHEDFPYSLQLARFIKTFAFYDNPFYQYRVLGGSISHNIK
                     YKNFSDVLTHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSKNIILIY
                     RKYFSFKEKCRKIFGAKTIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKFFSE
                     "
     gene            887..2224
                     /gene="ribB"
                     /locus_tag="WIE35_001903"
     CDS             887..2224
                     /gene="ribB"
                     /locus_tag="WIE35_001903"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ4154778.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRTEDGAALLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
BASE COUNT          636 a          712 c          540 g          531 t
ORIGIN      
        1 aggttgattt gatcctgcat ccctcgtccc tcaattacca cgtcatcccc aaaggggcgg
       61 acttttcgga taatgatttt gtccgccatt ttgaaacgct ggtggagggg cggtactata
      121 tcgccaacgc gtggacaaaa atcgtcaggc gggaaataat catcaaaaac aatctgtttt
      181 tcccaaaagg atacattcac gaggatttcc cgtacagttt gcaattggcg cgttttatca
      241 agacttttgc cttttacgat aacccttttt accagtaccg cgttctcggc ggctctatca
      301 gccacaacat caaatacaaa aatttcagcg atgtgctgac gcatctcgac cggggtgtgg
      361 attttttagt cgaaaacaaa aattccccca tctacggcgg tttgcaaaaa tttgtcttcg
      421 acaatatcgg ctatctgagg tctatattgg taaggcttta tttttccaaa aacattatcc
      481 tcatctaccg gaaatatttt tcatttaaag aaaaatgcag aaagatattc ggcgcgaaga
      541 caatccgtcc ggtttttatc gggaagaccg cattcatcat aggattgccg atattgcgcc
      601 tgctcgtacc gcctatgctg tacccggcaa tcaaggccgt ttatcagaaa tttttttcgg
      661 aataagcttc cggcaaaccc caaatcagaa gtgcgcggga agttgggaca aacagccccc
      721 ggccggcaag gattgcggca atgccgtctg aagccacgaa tccggcttca gacggcatct
      781 gtttaccaaa aagcaaataa ttcggtttgg cgaaaaaaac agatttgctt tttggtaaat
      841 acgcgattac aatccgctac atccgatttc tacaaaggat gaaacgatga ccgacacagc
      901 cggtctgcgc cgccgcaacc tgcggcaatg gatagcagaa cactacggcg gtttgcaaac
      961 ccgttttgcc gaagccgttg ccctcaacac aggcgaactc tccgcccttt tgaaaaacaa
     1021 atccttcggc gagaaaaaag cccgtaaaat cgaacaggcg gcaaaaatgc ccgccttctg
     1081 gctcgatacc gaacacaccg cccgcccgcc cgaacacaca ggaaaacaca ccatgtccca
     1141 tatttccccc atccccgaaa tcctagccga catcaaagcc ggcaaaatgg tcatcatcac
     1201 cgatgccgaa gaccgagaaa acgaaggcga cctgctgatg gcggcgcaat tcgtcacgcc
     1261 cgaagccatc aacttcatga tcaaacacgc gcgcggcttg gtctgcctgc cgatggacgg
     1321 cgaaatggtc gaaaaactcg ggctgccgat gatgacccaa aaaaacggcg cgcaatacgg
     1381 caccaacttt accgtctcca tcgaagccgc acacggcatt accaccggca tttccgccgc
     1441 cgaccgcgcc ctgactattc aaaccgccgt ttccccgacc gctaaacccg aagacatcgt
     1501 ccaacccggt catatctttc cgcttcgcgc ccaaaaaggc ggcgtactcg tccgcgccgg
     1561 acacaccgaa gccggcgtcg acctggcgca aatgaacggg ctgattcctg cctccgttat
     1621 ttgcgaaatc atcaacgacg acggcacgat ggcgcgtatg cccgaactga tgaaattcgc
     1681 cgaagaacac aagctcaaaa tcggcacgat tgccgacctc atcgaatacc gcagccgtac
     1741 cgaaagcctg cttgaagaca tgggcaatgc gcctgtacaa accccgtggg gcgagttcca
     1801 acaacacgtt tacgtcgaca aactctccgg cgaaacccac ctcgccctcg tcaaaggcac
     1861 gccctccgcc gacaccgaaa ccctcgtccg cgtccacgaa cccttcagcg tcatggactt
     1921 catccaagcc aacccgcgcc attcatggtc gctgcccaaa gcactcgagc gcatccaaca
     1981 ggccgaaagc ggcgtagtca tccttctgca ccgcaccgaa gacggcgccg ccctgctcga
     2041 ccgcaccctg cccaaaggtg caaaccaagc ctacaaatgg gacagcaaaa gctacggcat
     2101 cggcgcacaa atcctcgtcg gcctcaacgt caaaaaactg cgcgtcctcg ggcagccctc
     2161 atctttcacc ggcctgaccg gcttcggttt ggaagtcgtc ggctttgaag aagcggaaaa
     2221 ataatatagt aaattcaaat actttatatt tgctttattt attgcattat ttccgtgcaa
     2281 acgaaaaccc ggtctgttgg gttggatttt gtttttgcaa atttcgggta acttctaatt
     2341 cgtcattccc gcgcaggcgg gaatccggtt cgtcgggttt ttgtcatttc cgataaattc
     2401 ctgtggcttt ggttttttg
//
LOCUS       DAWHXZ010000238         2380 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.238, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000238 DAWHXZ010000000
VERSION     DAWHXZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.238"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            52..1179
                     /gene="bamC"
                     /locus_tag="WIE35_001904"
     CDS             52..1179
                     /gene="bamC"
                     /locus_tag="WIE35_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ4154779.1"
                     /translation="MTHIKPVIAALALIGLAACSGSKTEQPKLDYQSRSHRLIKLEVP
                     PDLNNPDQGSLYRLPAGSGAVRASDLEKRRTPAVQQPADAEVLKSVKGVRLERDGSQR
                     WLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIGQMETEWAENRAKIPQDSLRRLFD
                     TVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHKAMKEVYGGKDKDTTVWQPSPSDP
                     NLEAAFLTRFMQYLGVDGQQAENASAKNPTLPAANEMARIEGKSLIVFGDYGRNWRRT
                     ALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTEQKPGLFKRLLGKGKAEKPAEQPE
                     LIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGKLHSELR"
     gene            complement(1236..2168)
                     /gene="pip"
                     /locus_tag="WIE35_001905"
     CDS             complement(1236..2168)
                     /gene="pip"
                     /locus_tag="WIE35_001905"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ4154780.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKDWADWESYLICFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIQHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT          643 a          766 c          529 g          442 t
ORIGIN      
        1 ggacggcata acgcaacaaa cactttaata tccatcacac aggatgacac gatgacccat
       61 atcaaacccg tcattgccgc gctcgcactc atcgggcttg ccgcctgctc cggcagcaaa
      121 accgaacagc ccaagctcga ctaccaaagc cggtcgcacc gcctgatcaa acttgaagtc
      181 ccacccgatt tgaacaaccc cgaccaaggc agcctctacc gcctgcctgc cggttcgggc
      241 gccgtccgcg ccagcgattt ggaaaaacgc cgcacacccg ccgtccaaca gcctgccgat
      301 gccgaagtat tgaaaagcgt caaaggtgtc cgcctcgagc gcgacggcag ccaacgttgg
      361 cttgtcgttg acggcaaatc ccctgccgaa atctggccgc ttctgaaagc cttttggcag
      421 gaaaacggct tcgacatcaa atccgaagaa cccgccatcg gacaaatgga aaccgagtgg
      481 gcggaaaacc gtgccaaaat cccccaagac agcttgcgcc gcctattcga cacagtcggt
      541 ttgggcggca tctactccac cggcgagcgc gacaaattca tcgtccgtat cgaacagggc
      601 aaaaacggcg tttccgacat cttcttcgcc cacaaagcca tgaaagaagt gtacggcggc
      661 aaagacaaag acacgaccgt atggcagccc tccccgtccg atcccaacct cgaagccgct
      721 ttcctgacgc gctttatgca atatttgggc gttgacggac agcaggcgga aaacgcatcg
      781 gcaaaaaacc cgaccctgcc cgccgccaac gaaatggcgc gtatcgaagg caaaagcctg
      841 attgtctttg gcgactacgg cagaaactgg cggcgcaccg cgctcgccct cgaccgcatc
      901 gggctgaccg tcgtcggtca aaacaccgaa cgccacgctt tcctggttca aaaagccccg
      961 aacgaaagca atgcagttac cgaacaaaaa cccggcctgt tcaaacgcct gctgggcaaa
     1021 ggcaaagcgg agaaacctgc cgaacagccg gaactgattg tctatgccga gcctgtcgcc
     1081 aacggctcgc gcatcgtcct gctcaacaaa gacggcagcg catatgccgg caaagacgca
     1141 tccgcattat tgggcaaact ccattccgaa ctgcgttaaa ccattcaaaa caaaagcagc
     1201 ttccaaacag aagctgcttt tttatgcgga actttttaca acaaatgggg caaaatatcc
     1261 tcaactgcct gaaccaacgc atccaccaaa ggcggatcga acgcacgatg ccctgcctga
     1321 accaccctca attccgcttc gggaaaggct ttcgacagcg cccacgcact ctgcatcggc
     1381 gtacacaaat cataccgccc ctgcacgata atggtcggga tatgctgtat tttgccgata
     1441 ttgttcaaaa tcgccctatc gccctgcaac cagccgccgt tgacaaaata atggttttcc
     1501 aaacgcgcga ttgccagcga ggcataagca tcttcatcca cttcctccgg ctcgaaacag
     1561 atcagatagc tttcccaatc cgcccaatcc ttcgcggcag acaggcacac ttcttcatct
     1621 tgatgaaaca gcaacccgtg atacgcctca atcagccggt tccgccgatt ttcagcaatc
     1681 ggcgcgacaa atttttgcca ttgttccgga taaatccggc tcacaccgcc cgcctcgttc
     1741 agccacgccg tttcagacgg cctgcacaaa aatatcccgc gcaacaccaa tccctttacc
     1801 cgttccggat gggtttgggc ataagccagc gacaaagtgc tgccccacga accgccgaac
     1861 accagccatt tcccgatacc cagcatttca cggacttttt caatatccgc caccaaatcc
     1921 caagtcgtat tgtcttccgc acaagcatac gggcgcgaac gtccgcaacc gcgctggtcg
     1981 atgatgacga tgcggaacac atcgggatta aaaaaacccc gacattcagg cgaagccccc
     2041 gcgcccggcc cgccatgtaa aaaaataacc ggcacaccgt cgggattgcc cgattcctcc
     2101 caataaattt gatgaatttc agacacctgc aagtatccgc tatgaaaagg ctgttttatc
     2161 tcatacatca tcttctccca tattgcttct gataatccga acattcccac acgcaacatc
     2221 acgcacgaga cacaagccgc cccgaaaggc aaatgccgaa aaatcgcgat atagtgaatt
     2281 aacaaaaatc aggacaaggc ggcgaagcct cagacagtac agatagtacg gaaccgattc
     2341 acttggtgct tcagcacctt agagaatcgt tctctttgag
//
LOCUS       DAWHXZ010000239         2369 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.239, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000239 DAWHXZ010000000
VERSION     DAWHXZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.239"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            268..2070
                     /locus_tag="WIE35_001906"
     CDS             268..2070
                     /locus_tag="WIE35_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ4154781.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVATAVLATLLS
                     ATVQANATDEEDDEELEPVVRTAPVLSFHSDKEGTGEKEEVGASSNLTVYFDKKGVLK
                     AGTITLKAGDNLKIKQNTNENTNENTNASSFTYSLKKDLTDLTSVGTEKLSFSANGNK
                     VNITSDTKGLNFAKETAGTNGDTTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKK
                     RAASVKDVLNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGK
                     KTEVKIGAKTSVIKEKDGKLVTGKDKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKT
                     TTANGQTGQADKFETVTSGTKVTFASGNGTTATVSKDDQGNITVKYDVNVGDALNVNQ
                     LQNSGWNLDSKAVAGSSGKVISGNVSPSKGKMDETININAGNNIEITRNGKNIDIATS
                     MTPQFSSVSLGAGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQL
                     KGVAQNLNNRIDNVDGNARAGIAQAIATAGLAQAYLPGKSMMAIGGGTYRGEAGYAIG
                     YSSISDTGNWVIKGTASGNSRGHFGTSASVGYQW"
     gene            2203..>2369
                     /locus_tag="WIE35_001907"
     CDS             2203..>2369
                     /locus_tag="WIE35_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ4154782.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYP"
BASE COUNT          734 a          594 c          575 g          466 t
ORIGIN      
        1 ccacaggaat ctatcagcaa aaacagaaac cccaccgccg tcattcccgc aaaagcggga
       61 atccagaccc gtcggcacgg aaacttaccg aataaaacag tttccttaga ttccacgtcc
      121 cagattcccg ccttcgcggg aatgacgaga ttttaagttg ggggaattta tcagaaaacc
      181 cccaacccca aaaaaccggg cggatgccgc accatccgcc cccaaacccc gatttaacca
      241 ttcaaacaaa ccaaaagaaa aaacaaaatg aacaaaatat accgcatcat ttggaatagt
      301 gccctcaatg cctgggtcgt cgtatccgag ctcacacgca accacaccaa acgcgcctcc
      361 gcaaccgtgg cgaccgccgt attggcgaca ctgctgtccg caacggttca ggcgaatgct
      421 accgatgaag aagacgatga agagttagaa cccgtagtac gcactgctcc cgtgttgagc
      481 ttccattccg ataaagaagg cacgggagaa aaagaagaag taggagcaag ttcaaatttg
      541 acagtatatt tcgacaagaa aggagtacta aaagccggaa caatcaccct caaagcaggc
      601 gacaacctga aaatcaaaca aaacaccaat gaaaacacca atgaaaacac caatgccagt
      661 agcttcacct actcgctgaa aaaagacctc acagatctga ccagtgttgg aactgaaaaa
      721 ttatcgttta gcgcaaacgg caataaagtc aacatcacaa gcgacaccaa aggcttgaat
      781 tttgcgaaag aaacggctgg gacgaacggc gacaccacgg ttcatctgaa cggtattggt
      841 tcgactttga ccgatacgct gctgaatacc ggagcgacca caaacgtaac caacgacaac
      901 gttaccgatg acgagaaaaa acgtgcggca agcgttaaag acgtattaaa cgcaggctgg
      961 aacattaaag gcgttaaacc cggtacaaca gcttccgata acgttgattt cgtccgcact
     1021 tacgacacag tcgagttctt gagcgcagat acgaaaacaa cgactgttaa tgtggaaagc
     1081 aaagacaacg gcaagaaaac cgaagttaaa atcggtgcga agacttctgt tattaaagaa
     1141 aaagacggta agttggttac tggtaaagac aaaggcgaga atggttcttc tacagacgaa
     1201 ggcgaaggct tagtgactgc aaaagaagtg attgatgcag taaacaaggc tggttggaga
     1261 atgaaaacaa caaccgctaa tggtcaaaca ggtcaagctg acaagtttga aaccgttaca
     1321 tcaggcacaa aagtaacctt tgctagtggt aatggtacaa ctgcgactgt aagtaaagat
     1381 gatcaaggca acatcactgt taagtatgat gtaaatgtcg gcgatgccct aaacgtcaat
     1441 cagctgcaaa acagcggttg gaatttggat tccaaagcgg ttgcaggttc ttcgggcaaa
     1501 gtcatcagcg gcaatgtttc gccgagcaag ggaaagatgg atgaaaccat caacattaat
     1561 gccggcaaca acattgagat tacccgcaac ggtaaaaata tcgacatcgc cacttcgatg
     1621 accccgcagt tttccagcgt ttcgctcggt gcgggggcgg atgcgcccac tttaagcgtg
     1681 gatgacgagg gcgcgttgaa tgtcggcagc aaggatgcca acaaacccgt ccgcattacc
     1741 aatgtcgccc cgggcgttaa agagggggat gttacaaacg tcgcacaact taaaggtgtg
     1801 gcgcaaaact tgaacaaccg catcgacaat gtggacggca acgcgcgtgc gggcatcgcc
     1861 caagcgattg caaccgcagg tttggctcag gcgtatttgc ccggcaagag tatgatggcg
     1921 atcggcggcg gtacttatcg cggcgaagcc ggttacgcca tcggctactc gagcatttct
     1981 gacactggga attgggttat caagggcacg gcttccggca attcgcgcgg tcatttcggt
     2041 acttccgcat ctgtcggtta tcagtggtaa gggctttttc gcctgtctgc tgttggggca
     2101 ggcggaaggt ttgaagggaa gggtggcgat ttgccgcctg agacctttgc aaaattcccc
     2161 aaaatcccct aaattcccac caagacattt aggggatttt ccatgagcac cttcttccaa
     2221 caaaccgccc aagccatgat tgccaaacac atcgaccgtt tcccgctatt gaagttggat
     2281 caggtaattg attggcagcc gatcgagcag tacctgaacc gtcaaaaaaa ccgttacctc
     2341 cgagaccacc gcggccgtcc tgcctatcc
//
LOCUS       DAWHXZ010000240         2320 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.240, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000240 DAWHXZ010000000
VERSION     DAWHXZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.240"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            26..2320
                     /gene="rnr"
                     /locus_tag="WIE35_001908"
     CDS             26..2320
                     /gene="rnr"
                     /locus_tag="WIE35_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ4154783.1"
                     /translation="MPSREWIIELLERKGVPSKIESLARELSITEDEYVFFERRLKAM
                     ARDGQVLINRRGAVCAADKLDLVKCRVEAHKDGFGFAVPLTPAKDGDFVLYERQMRGI
                     MHGDIVTVRPAGIDRRGRREGTVLDIVERAQSKVVGRFYMDRGVAILEPEDKRLNQSI
                     VLEPDGVARFKPESGQVIVGEIEVYPEQNRQAVAKIIEVLGDYADSGMEIEIAVRKHH
                     LPHQFSEACAKAVKKIPDHVRKSDLKGRVDLRDLPLVTIDGETARDFDDAVFAEKIGR
                     NYRLVVAIADVSHYVRPDDAIDTDAQERSTSVYFPRRVIPMLPENLSNGICSLNPNVE
                     RLCMVCDMVITYAGNIKEYRFYPAVMRSHARLTYNQVWKWLSGGIGHPFKTQIDTLYK
                     LFKILQKKRFERGAVEFDSIETQMLFDDNGKIEKIVPVVRNDAHKLIEECMLAANVCA
                     ADFLLKNKHTALFRNHLGPTPEKLATLREQLGLLGLQLGGGDNPSPKDYAALVEQFKG
                     RPDAELLQVMMLRSMQQAVYEPHCDGHFGLAYEAYAHFTSPIRRYPDLTVHRAIKAVL
                     NQQTYTPKKSWQALGVHTSFCERRADDASRDVENWLKTYYMRDKVGEVFEGKISGMTS
                     FGIFVTLDGIHIDGLVHISDLGEDYFNFRPEIMAIEGERSGIRFNMGDRVAVRVARAD
                     LDDGKIDFVLIAGGSGRGRKVKSSASAKPAGTAGKGKPKTAAEKKTARGGKVRGRGAS
                     AAAESGKKAKKPVPIKVKKRKGKS"
BASE COUNT          577 a          599 c          657 g          487 t
ORIGIN      
        1 tgaaaaacag cgttatgaac atcctttgcc cagtcgggaa tggataatcg aattgttgga
       61 gcgcaaaggt gtgccttcaa aaatcgaatc gcttgcgcgc gagctgtcga ttacggaaga
      121 cgagtatgtc tttttcgaac gccgtctgaa ggcgatggcg cgggacggtc aggttttaat
      181 caaccgtcgg ggcgcggttt gcgcggcgga caaattggat ttggtcaaat gccgcgtcga
      241 ggcgcataag gacggtttcg gttttgccgt gccgctcacg cccgccaaag acggtgattt
      301 tgttttgtat gaacgccaga tgcgcggcat tatgcacggc gatattgtca ctgttcgtcc
      361 tgccggcatc gaccgtaggg gccgccgcga agggacagtt ctggatattg tcgaacgcgc
      421 gcaaagcaaa gtggtcggcc gtttctatat ggataggggc gtggcgattt tggagccgga
      481 agacaagcgt ctgaaccaaa gcatcgtatt ggaaccggac ggcgtggcgc gtttcaaacc
      541 tgaatccggt caggtcatcg tcggcgaaat tgaggtttat cctgagcaaa accggcaggc
      601 agtggcaaaa atcatcgaag ttttgggcga ttatgccgac agcggcatgg agattgaaat
      661 tgccgtgcgc aagcatcatt tgccgcacca attcagtgaa gcgtgtgcca aagccgtgaa
      721 aaaaattccc gaccatgtac gcaaaagcga tttgaaaggc cgcgtcgatt tgcgcgacct
      781 gcctttggta acgattgacg gcgaaacggc gcgcgatttt gacgatgcgg tgtttgccga
      841 aaaaatcgga cgcaattacc gtctggtcgt ggcgattgcc gatgtcagcc attatgtccg
      901 ccccgatgac gctatcgaca cggacgctca ggaacgcagc accagtgttt acttcccgcg
      961 ccgcgtgatt cccatgttgc cggaaaacct gtccaacggc atctgctcgc tcaatcctaa
     1021 tgtcgagcgt ttgtgtatgg tgtgcgatat ggtcatcact tacgcgggca atattaaaga
     1081 ataccgcttc taccccgccg tgatgcgctc tcatgcccgc ctgacctaca accaagtttg
     1141 gaaatggctt tcaggcggca tcgggcatcc gttcaaaaca caaatcgaca cgctttacaa
     1201 actcttcaaa atccttcaga aaaagcgttt cgaacgcggg gcggtggagt ttgacagcat
     1261 cgaaacccaa atgcttttcg acgacaacgg taaaattgaa aaaatcgtcc ccgttgtccg
     1321 caacgatgcc cacaagctga ttgaagaatg tatgctggcg gcgaatgttt gcgcggcgga
     1381 ttttctgttg aaaaacaagc atacggcttt gttccgcaac catttgggcc ccacgcccga
     1441 aaaactcgcc accctgcgcg agcagctcgg tctgttgggg cttcaacttg gcggcggcga
     1501 caacccgtcg ccgaaagact atgccgcgct tgtcgaacaa ttcaaaggca gacctgatgc
     1561 cgaattgctg caagtcatga tgttgcgctc catgcaacag gcggtttacg aaccgcattg
     1621 cgacggacac tttggtcttg cctacgaagc atacgcccac ttcacctcgc ccatccgccg
     1681 ctatcccgac ctgaccgtac accgcgccat caaagccgtg ttgaatcagc aaacctacac
     1741 gccaaaaaaa agctggcagg ctttgggcgt gcatacctcg ttctgtgagc gccgtgccga
     1801 cgacgccagc cgcgacgtgg aaaactggct gaaaacctat tatatgcgcg ataaggtcgg
     1861 cgaagtattc gaaggtaaaa tctccggcat gaccagtttt ggtatctttg taacactgga
     1921 cggcatccac attgacggct tggtgcatat cagcgatttg ggcgaagact atttcaactt
     1981 ccgccccgaa atcatggcaa tcgaaggcga acgcagcggc atccgtttca acatggggga
     2041 cagggttgcc gtccgggtcg cccgtgccga tttggatgac ggaaaaatcg attttgtcct
     2101 gattgccggg gggagcggca gggggcggaa agttaaatca tccgcgtctg ccaaaccggc
     2161 agggacggcg gggaaaggga agccgaaaac cgccgccgag aaaaaaacag cccgaggcgg
     2221 caaagtaagg ggaaggggcg cgtctgccgc cgcagaatcg gggaaaaagg caaagaaacc
     2281 ggttccgatt aaggtaaaaa aacggaaagg caaatcataa
//
LOCUS       DAWHXZ010000241         2301 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.241, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000241 DAWHXZ010000000
VERSION     DAWHXZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.241"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1383
                     /gene="guaA"
                     /locus_tag="WIE35_001909"
     CDS             <1..1383
                     /gene="guaA"
                     /locus_tag="WIE35_001909"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ4154784.1"
                     /translation="SDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQREFGY
                     AQVKTIDSELTRDIFDDAPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMENAEK
                     QFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSDEVI
                     LGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVIHVD
                     AEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAKEKKLTNAKWLAQGTIYPDVIESAGA
                     KTQKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHPFPG
                     PGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPVKSV
                     GVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVYDVS
                     GKPPATIEWE"
     gene            1445..2191
                     /gene="fabG"
                     /locus_tag="WIE35_001910"
     CDS             1445..2191
                     /gene="fabG"
                     /locus_tag="WIE35_001910"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ4154785.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT          575 a          687 c          608 g          431 t
ORIGIN      
        1 tccgactatc aggccgatac cggtattttt gatttgggca ttccggtttt gggcatctgc
       61 tacggaatgc agtttatggc gcaccacttg ggcggcgaag tgcagcccgg caaccagcgc
      121 gaattcggtt acgcgcaagt caaaaccatc gacagcgaac tgacccgcga tattttcgac
      181 gacgcgccca acacactcga cgtatggatg agccacggcg acaaagtgtc caaactgccc
      241 gacggcttcg ccgtcatcgg caacaccccg tcctgcccga ttgccatgat ggaaaacgcc
      301 gaaaaacaat tctacggcat ccagttccac cccgaagtta cccacaccaa acaaggccgc
      361 gccctgttga accgctttgt cttggatatt tgcggcgcac aaccgggctg gacgatgccg
      421 aactacatcg aagaagccgt tgccaaaatc cgcgaacagg tcggcagcga cgaagtgatt
      481 ttaggtttgt caggcggcgt agactcttcc gtagccgccg cgctgattca ccgcgccatc
      541 ggcgaccaac tgacctgcgt gttcgtcgat cacggtttgt tgcgcctgaa cgaaggcaaa
      601 atggtgatgg atatgttcgc ccgcaacttg ggtgtgaaag tgattcacgt cgatgccgaa
      661 gggcagttta tggcgaaact cgccggcgtt accgaccctg agaaaaaacg caaaatcatc
      721 ggcgcggaat ttatcgaagt atttgatgcc aaagagaaaa aactcaccaa cgccaaatgg
      781 ctggcgcaag gcacgattta ccccgacgta atcgaatccg caggcgcgaa aacccaaaaa
      841 gcccacgcca tcaaatcgca ccacaacgtc ggcggtctgc ccgaaaatat gaagctcaag
      901 ctgcttgagc cgttgcgcga cttgtttaaa gacgaagtgc gcgaactggg cgttgctttg
      961 ggtctgccgc gcgaaatggt gtaccgccat ccgttccccg gccccggttt gggcgtgcgc
     1021 atcttgggcg aagtgaaaaa agaatacgcc gacttattgc gtcaagcaga cgatattttt
     1081 attcaagaat tacgcaacac caccgacgaa aacggcacgt cttggtacga cctgaccagc
     1141 caagccttcg ccgtgttcct gcctgtgaaa tccgtcggcg taatgggcga cggacgcact
     1201 tacgactacg tcgtcgcact gcgcgcagtc atcaccagcg actttatgac cgcacattgg
     1261 gctgagttgc cttactcact gctcggacgc gtgtccaacc gcatcatcaa cgaagtcaaa
     1321 ggcatcaacc gcgtggtgta cgatgtcagc ggcaaaccgc ctgccaccat cgagtgggaa
     1381 taaccagcaa acagggctgc accgtccggc gcagtctttc gattatcgga aaaaaggaaa
     1441 aaatatgagc acacaagatt taagcggcaa aatcgctttg gtaacaggcg cctcgcgcgg
     1501 tatcggtgca gcaattgccg acacactggc ggcagccggt gccaaagtca tcggtacggc
     1561 gaccagtgag agcggtgcgg cggcgattag cgagcggttg gcgcaatggg gcggcgaagg
     1621 ccgcgtatta aattccgccg aacccgaaac cgtcgaaaac ctgattgccg acatcgaaaa
     1681 aacgttcggc aaactcgata ttctggtcaa caacgccggc atcacccgcg acaacctcct
     1741 gatgcgtatg aaagaagaag agtgggacga catcatgcag gtcaacctca aatccgtctt
     1801 tcgcgcctcc aaagccgtct tgcgcggcat gatgaagcag cgcgccggcc gtatcatcaa
     1861 catcacatcc gtcgtcggcg tgatgggcaa tgccggtcaa accaactatg ccgcggcaaa
     1921 agcaggctta atcggtttct ccaaatccat ggcgcgcgaa gtcggcagcc ggggcattac
     1981 cgtcaactgc gtcgcccccg gctttatcga taccgacatg acccgcgccc tgccggaaga
     2041 aacccgccaa acctttaccg cccaaaccgc cttgggcaga ttcggcgacg cgcaagacat
     2101 tgccgatgca gtcttgttct tggcttccga tcaggcaaaa tacattaccg gccagacgct
     2161 gcacgtcaac ggcggcatgc tgatgccctg acggacagcc gtgccgacaa tgccgtctga
     2221 agccctttca gacggcattt gcattcccaa acaaaatgaa cacacgccac accccgccct
     2281 gcccctgcgg atcaggcaca a
//
LOCUS       DAWHXZ010000242         2182 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.242, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000242 DAWHXZ010000000
VERSION     DAWHXZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.242"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            126..1235
                     /locus_tag="WIE35_001911"
     CDS             126..1235
                     /locus_tag="WIE35_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ4154786.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
     gene            1244..2071
                     /gene="fghA"
                     /locus_tag="WIE35_001912"
     CDS             1244..2071
                     /gene="fghA"
                     /locus_tag="WIE35_001912"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ4154787.1"
                     /translation="MKLIEQHQIFGGLQQVWAHDAQTLQCEMKFAVYLPNNSENQPLG
                     VIYWLSGLTCTEQNFITKSGFQLYAAEHQVIVVAPDTSPRGEQVPNDAAYDLGQGAGF
                     YLNATEQPWATNYQMYDYILNELPDLVEANFSTNGKRSIMGHSMGGHGALVLALRNRK
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGEDREKWQQYDASSLIQQGYKVQGIRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
BASE COUNT          598 a          459 c          557 g          568 t
ORIGIN      
        1 cagtaaaaaa cacttgcctt agagttaact ctaaagtgta aactgttgct atgttgctca
       61 ggtaaggcaa ctttgtcaat gaattaagag gaaagacaat ggaaatgaaa caaaccgatt
      121 caagcatcaa atctcgtgcg gcggtggcat tcgcccctaa ccaaccctta caaattgtgg
      181 aaatcgacgt agaaatgccg cgtaaaggcg aggtgttaat ccgcaatacc cacactggcg
      241 tgtgccatac tgatgcgttt acattatcag gaagcgatcc tgaaggcgta ttccctgtgg
      301 tgcttggaca cgaaggtgcg ggtgtggtcg ttgctgtggg cgaaggtgtg tcaagcgtaa
      361 aaccgggtga tcacgtgatt ccgctttaca ccgccgaatg tggcgaatgt gagttttgtt
      421 gttcaggtaa aaccaacttg tgcgtctcag tgcgtgatac acaaggtaaa ggcttaatgc
      481 cggacggcac gacgcgtttt tcttatcaag gtcagccgat ttatcactat atgggctgtt
      541 cgactttcag tgaatactcc gttgttgccg aagtttcact ggcgaaaatc aaccctgaag
      601 ccaaccatga acaagtatgt ttgctcggct gcggcgttac cacaggtatt ggtgcggtac
      661 ataatacggc aaaagtgcaa gaaggcgact ctgttgccgt gtttggctta ggcgcgattg
      721 gtttggcggt ggtgcaaggt gcgcgtcaag ccaaagccgg ccgcattatc gccattgata
      781 ccaatccatc aaaatttgag ttggcaaaac agttcggtgc aacggattgt ttgaacccga
      841 acgattacga taaaccaatt aaagatgtgt tgttagacat caacaaatgg ggcattgacc
      901 atacctttga atgtatcggc aatgtaaacg taatgcgtca agcattagaa agtgcacatc
      961 gtggttgggg tcaatccatt atcatcggcg tagcaggtgc aggacaagaa atttcaacgc
     1021 gtccgttcca gttggtaaca ggccgtgtgt ggaaaggttc agcatttggc ggtgtgaaag
     1081 gtcgctctga actgccgaaa atggtggaag attcaatgaa aggcgacatt gagttagaac
     1141 cgtttgtaac ccacacaatg acactcgatc aaatcaataa agcctttgac ttaatgcacg
     1201 aaggtaaatc gatccgcgcc gttattcact actaaggtat gcgatgaaac tgattgaaca
     1261 acatcaaatt tttggcggtt tgcagcaagt ttgggctcat gatgcccaaa cgcttcaatg
     1321 cgagatgaaa tttgccgtct atttgccaaa taattcggaa aatcaaccgc ttggtgtgat
     1381 ttattggctt tccggcttga cgtgtacgga acaaaatttc attactaaat caggctttca
     1441 gctttatgca gcagaacatc aagtgattgt ggttgcccct gataccagcc ctcgcggaga
     1501 gcaagtgccg aacgatgcgg cttacgattt aggacaaggt gcgggctttt atcttaatgc
     1561 aactgagcag ccttgggcga cgaattatca aatgtatgat tatatcttga acgaattgcc
     1621 tgatttggtt gaagcaaatt tctctactaa cggcaaacgt tccattatgg ggcattcaat
     1681 gggcggacac ggtgcattgg tattggcact gcgaaatcgg aaacgttatc aaagcgtttc
     1741 tgctttttcg cctattttat cgccaagcct cgtgccttgg ggagaaaagg cctttactgc
     1801 ttatttaggg gaagatcgtg aaaaatggca gcaatatgat gccagctcgc tcattcaaca
     1861 aggctataaa gtgcaaggta tacgcattga tcagggcttg gaagatgagt ttttgccgac
     1921 acaattgcgt accgaagatt ttatcgaaac ctgtcgtgcg gcaaaccagc cggtcgatgt
     1981 gcgcttccac aaaggctacg accatagtta ttacttcatc gccagtttta ttggcgaaca
     2041 tattgcttat cacgccgcgt ttttgaagta aaccaaagag cgttcagtgt tcaaagcagt
     2101 tttgggatag ccggcacgag ggcggtaaga agtgccggca taaacgtatg ccgtctgagc
     2161 cggaaggagc cgactctacg ga
//
LOCUS       DAWHXZ010000243         2172 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.243, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000243 DAWHXZ010000000
VERSION     DAWHXZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.243"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            104..1915
                     /gene="typA"
                     /locus_tag="WIE35_001913"
     CDS             104..1915
                     /gene="typA"
                     /locus_tag="WIE35_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ4154788.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQVVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          544 a          622 c          564 g          442 t
ORIGIN      
        1 gaaaggcaag ggcttcagac ggcatcttca tttcccaaat actgtccggt aaagcgtggt
       61 ataatgcgca gttatctctt acagtttttg aaaaacatta attatgaaac aaatccgcaa
      121 catcgccatc atcgcccacg tcgaccacgg caaaaccaca ttggtcgacc aactgctgcg
      181 ccaatccggc acattccgcg ccaaccagca ggttgacgag cgcgtgatgg acagcaacga
      241 cctcgaaaaa gaacgcggca tcaccatcct cgccaaaaac accgccatcg attacgaagg
      301 ctaccacatc aatatcgtcg acacgccggg acacgccgac ttcggcggcg aagtggagcg
      361 cgttttgggg atggtggact gcgtcgtctt gttggtggac gcgcaggaag gcccgatgcc
      421 gcaaacccgt ttcgtgacca aaaaagcctt ggctttgggg ctgaaaccga ttgtcgtcat
      481 caataaaatc gacaaaccgt ccgcccgtcc gagctgggtc atcgaccaaa ctttcgagct
      541 gttcgacaac ttgggcgcga ccgacgagca gttggatttc ccgattgttt atgcttccgg
      601 tctgtccggt ttcgccaaat tggaagaaac cgacgagagc aacgacatgc gtccgctgtt
      661 cgatactatc ttaaaatata cgcctgcacc gagcggcagc gcggacgaaa cgctgcaact
      721 gcaaatttcc caactcgact acgacaacta caccggccgc ctcggtatcg gtcgtatctt
      781 gaacggacgt atcaagcccg gtcaagttgt tgccgtcatg aaccacgatc aacaaatcgc
      841 ccaaggccgc atcaaccagc ttttgggttt caaaggttta gaacgcgtgc cgcttgaaga
      901 agccgaagcc ggcgacatcg tgattatttc cggtattgaa gacatcggca tcggcgtaac
      961 catcaccgac aaagacaacc ccaaaggcct gccgatgttg agcgtggacg aaccgacgct
     1021 gaccatggac tttatggtta acaccagccc gttggcgggt acggaaggca aattcgtaac
     1081 cagccgccaa atccgcgacc gcctgcaaaa agaattgctg accaacgtcg ccctgcgcgt
     1141 ggaagacacc gccgatgccg acgtgttccg cgtatccgga cgcggcgagc tgcacctgac
     1201 cattttgttg gaaaatatgc gccgcgaagg ctacgaactc gccgtcggca aaccgcgcgt
     1261 cgtgtaccgc gacatcgacg gtcaaaaatg cgaaccttat gaaaacctga ctgtggacgt
     1321 acccgacgac aaccaaggcg cggtaatgga agaactcggc cgccgccgtg gcgaactgac
     1381 taatatggaa agcgatggca acggacgcac ccgcctcgaa taccatattc cagcgcgcgg
     1441 cttgatcggt ttccaaggcg aattcatgac cctgacgcgc ggtgtcgggc tgatgagcca
     1501 cgtgttcgac gattacgcgc ccgtcaaacc cgatatgccc ggccgccaca acggcgtgct
     1561 ggtgtcccaa gagcagggcg aggcggtcgc ttacgccttg tggaatcttg aagaccgcgg
     1621 ccgtatgttc gtatcaccca acgacaaaat ctacgaaggt atgattatcg gcatccacag
     1681 ccgcgacaac gatttggtgg tcaacccgct caaaggcaaa aaacttacca acatccgcgc
     1741 cagcggcacc gacgaagccg tgcgcctgac cacgccgatc aagctgacgc tggaaggtgc
     1801 ggttgagttt atcgacgatg acgaactcgt tgaaatcacg ccgcaatcca tccgtctgcg
     1861 caagcgttac ttgagcgaat tggaacgccg ccgccacttt aaaaagctgg attgatgttt
     1921 actggattaa tgtttaaatg atacgcgatg ccgtctgaaa aatttcagac ggcatttttt
     1981 attcggacgg gctttgcggc ttcttgaagc tgtttcagac ggcgtttttc ctacccaatc
     2041 aagaaactgc cgccattttt ccagcggtat atcgccccgt cgcgtttcgg tatcgggttc
     2101 ggcttcccgg cagcatgtcg aacaatgccg tttgaaggaa tacgcctttt gagtccttac
     2161 aagcttagga ag
//
LOCUS       DAWHXZ010000244         2164 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.244, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000244 DAWHXZ010000000
VERSION     DAWHXZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.244"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..689)
                     /gene="cysT"
                     /locus_tag="WIE35_001914"
     CDS             complement(<1..689)
                     /gene="cysT"
                     /locus_tag="WIE35_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ4154789.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILP"
     gene            979..1311
                     /locus_tag="WIE35_001915"
     CDS             979..1311
                     /locus_tag="WIE35_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ4154790.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGTESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GEACACGH"
     gene            1643..2152
                     /locus_tag="WIE35_001916"
     CDS             1643..2152
                     /locus_tag="WIE35_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ4154791.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          599 a          500 c          533 g          532 t
ORIGIN      
        1 ggcaggattt cagaaaccat cggaatattg cccgcgataa aaatcaccga gccgtattcc
       61 cccgttgccc gcgcgaacat cattcccgcg ccagtcaaga gtgccggcgt gatttccggc
      121 agcagcacgc ggcggaacgt cgtaaagcgg tttgcgccca aagtcgccgc cgcttcctca
      181 tattcgcccg ataattcctc caacaccggc tgcacggcgc ggacgataaa gggcaggctg
      241 acgacgacca gcgcaatcca aatgccgacg ggcgtaaacg cgattttgat gcccaaaggc
      301 tcgaaaaaac ggcctatcca accgttgggc gcatacaggg tcgccaacgc gatacccgta
      361 accgccgtcg gcagcgcaaa cggcaaatcg accagcgcgt tcgccagacc cttgcccggg
      421 aattcataac gcaccaatac ccacgccacc agcgtgccga acacgacatt ggtcagcatc
      481 gcataaaacg acatccgcaa actcagccag acggccgcca agacattggg ttcggcaatc
      541 gtgttccaaa agccgcccca gccgatttcc gccgccttcg ccgccatcat cgcaaacggc
      601 aagaccacaa gcagcgacag gcacaatacg gtcagaccaa ggctgagttt gaagccgggc
      661 agtacgccgg gcgttttgag cggtaacata aaacaatgct gaaaataagg aaaaggaagg
      721 actactttaa cgatgccgtc cgaaaaacgg aaagaatgga aagtttggtg caaagacgaa
      781 tttgttataa agcggttggc agtttctcaa gcgggcgcga tgttttaaaa tatagtggat
      841 taacaaaaat caggacaagg cgacgaagcc gcagacagta caaatagtac ggcaaggcga
      901 ggcaacgccg tactggttta aatttaatcc actataacac cttgttttga cggaaaacca
      961 tcatataaag gaacacttat gcagatttta tcttttcaac cggacattgc ggaacgtatg
     1021 ctggaaggta cggaaggcga gtcggtcaac gaaaacgcac aattcgtccg tacggacaac
     1081 ggctattgga ttgcgtggca tgaaggcgta gcggcactgc ttgcgcccga tacgccgccg
     1141 ggcattccct gtttttgggt ggaaggcacg gaaagccttg aagagttgtg cgtcatggtg
     1201 gaacgcggcg agtttgacga agtggaagag tttgacggcg atgacgacga atggctcgaa
     1261 acggcacagg gttgcgggca ccacggcgaa gcttgcgcct gcggacatta aaggcattgc
     1321 aggcttgccg caaggggtgc aaggctttgc cgttttttaa ataatcggtg gattgcatct
     1381 gtttgcaatg ggcggaacgc ttccctttgt attttattga ataaaaaaac ttatcttggt
     1441 attataatta aggcatcatc aattattttg ggatggcaat aaacgcaaag cattgatttg
     1501 cgccgattgc agacttatta tagcaggttg cggcgcggac ttaatgggaa ttgtttatag
     1561 agcatgatgg tctgaaacat cattaattaa aaataccatt aatccgattt atatttattt
     1621 caatttcaat ggaaaaacat caatgacaat gattttaagc attttaagcc tgttttttat
     1681 catcagactg ttatttttag ccgtctctat taaacatgaa aaagccttga ttgccaaagg
     1741 ggcgaaacaa tacggaaaaa ccaattccac gctgcttgcg gcagttcata cgctttatta
     1801 tttggcgtgt tttgtttggg tatggctttc tgacactgct tttaatggca tatccttgat
     1861 tggtacgctg acggtgatgg cttcgtttgt gatattgtca ttgattatta agcagttggg
     1921 ggagatttgg acggttaaaa tctatatttt accaaatcat caaattaatc gttcgtggtt
     1981 gtttaaaaca ttccgccacc ccaattattt tttaaacatc atacccgaac tgattggcat
     2041 cgccttatta tgtcaagcgt ggtatgtttt attgattggc ctgccgattt atttgctggt
     2101 cttatttaag cgtatccgac aagaagaaca ggcgatggca acactttttt aacccgtttc
     2161 atca
//
LOCUS       DAWHXZ010000245         2156 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.245, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000245 DAWHXZ010000000
VERSION     DAWHXZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.245"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            127..1512
                     /locus_tag="WIE35_001917"
     CDS             127..1512
                     /locus_tag="WIE35_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ4154792.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            1532..2050
                     /locus_tag="WIE35_001918"
     CDS             1532..2050
                     /locus_tag="WIE35_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ4154793.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT          459 a          652 c          578 g          467 t
ORIGIN      
        1 atcgttcaaa taaacaggaa tataacttca gacaaacaac ttaccgcccg atttgtgcta
       61 tcgttttcgc acaacttaaa aaaacctgac aattttgtac ttttattaca gagaaaggct
      121 ttacaaatgg acggctggac acagacgctg tccgcgcaaa ccctgttggg catttcggcg
      181 gcggcaatca tcctcattct gattttaatc gtcaaattcc gcatccacgc gctgctgaca
      241 ctggtcatcg tcagcctgct gacggctttg gcaaccggtt tgcccacagg cagcattgtc
      301 aacgacgtac tggtcaaaaa cttcggcggc acgctcggcg gcgtggcgct tctggtcggc
      361 ctgggcgcga tgctcggacg tttggtcgaa acatccggcg gcgcacagtc gctggcggac
      421 gcgctgatcc ggatgttcgg cgaaaaacgc gcaccgttcg cgctgggcgt tgcctcgctg
      481 attttcggct tcccgatttt cttcgatgcc ggactaatcg tcatgctgcc catcgtgttc
      541 gccaccgcac ggcgcatgaa acaggacgta ctgcccttcg cgcttgcctc catcggcgca
      601 ttttccgtca tgcacgtctt cctgccgccc catccgggcc cgattgccgc ttccgaattt
      661 tacggcgcga acatcggcca agttttgatt ttgggtctgc cgaccgcctt catcacatgg
      721 tatttcagcg gctatatgct cggcaaagtg ttggggcgca ccatccatgt tcccgttccc
      781 gaactgctca gcggcggcac gcaagacaac gacctgccga aagaacctgc caaagcagga
      841 acggtcgtcg ccatcatgct gattcccatg ctgctgattt tcctgaatac cggcgtatcg
      901 gccctcatca gcgaaaaact cgtaagtgcg gacgaaacct gggttcagac ggcaaaaata
      961 atcggttcga caccgatcgc ccttctgatt tccgtattgg tcgcactgtt tgtcttggga
     1021 cgcaaacgcg gcgaaagcgg cagcgcgttg gaaaaaaccg tggacggcgc actcgccccc
     1081 gtctgttccg tgattctgat taccggcgcg ggcggtatgt tcggcggcgt tttgcgcgct
     1141 tccggcatcg gcaaggcact cgccgacagc atggcggatt tgggcattcc cgtccttttg
     1201 ggctgtttcc ttgtcgcctt ggcactgcgt atcgcgcaag gttcggcaac cgtcgccctg
     1261 accaccgccg ccgcgctgat ggctcctgcc gttgccgccg ccggctttac cgactggcag
     1321 ctcgcctgta tcgtattggc aacggcggca ggttcggtcg gttgcagcca cttcaacgac
     1381 tccggcttct ggctggtcgg ccgcctcttg gacatggacg taccgaccac gctgaaaacc
     1441 tggacggtca accaaaccct catcgcactc atcggctttg ccttgtccgc actgctgttc
     1501 gccatcgtct gacagacgga aaggatagta aatgactacg cattttgtcg ttatgggcgt
     1561 atgcggctgc ggcaagacca ccgccgcgct gtccctgcag aaacacctcg gtcaatgtcc
     1621 ctatgccgaa ggcgacgagt tccacaccca agccaaccgc gacaagatgg gcgcgggtat
     1681 tccgctgacc gatgaagacc gctatccgtg gttgggcaat ctgcgcgact ggatgacgca
     1741 acaggcgcaa aacggtgcga accacaccat cgtaacctgt tccgccctca aacgcggcta
     1801 ccgcgacatt ctgcgcggag ccgaaggcaa agctgccttc atccacctca gtccgccgca
     1861 agacatcaac ctcgagcgca tgatgtcgcg caaaggacat tacatgaaag cagggatgct
     1921 cgattcgcaa ctggaaatcc tcgaggaact gggcgaaggc gaatacgggg tcaaaatcgc
     1981 caaccccggc acgcccgaag cggtcgaagc cgatattctg aactgggttg cctcggaaaa
     2041 cctgctttga agcaatatgc cgtctgaagc ccgacacagg atgggtttca gacggcataa
     2101 acatcgggaa cagaatggat tacattgatt tatagtggat taacaaaaac cagtac
//
LOCUS       DAWHXZ010000246         2124 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.246, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000246 DAWHXZ010000000
VERSION     DAWHXZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.246"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            301..678
                     /locus_tag="WIE35_001919"
     CDS             301..678
                     /locus_tag="WIE35_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4154794.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            767..1336
                     /gene="rsmD"
                     /locus_tag="WIE35_001920"
     CDS             767..1336
                     /gene="rsmD"
                     /locus_tag="WIE35_001920"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ4154795.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVFSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PNIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            1459..1710
                     /locus_tag="WIE35_001921"
     CDS             1459..1710
                     /locus_tag="WIE35_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ4154796.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            1807..1890
                     /locus_tag="WIE35_001922"
     tRNA            1807..1890
                     /locus_tag="WIE35_001922"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1841..1843,aa:Tyr,seq:gta)
     gene            1921..1994
                     /locus_tag="WIE35_001923"
     tRNA            1921..1994
                     /locus_tag="WIE35_001923"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1953..1955,aa:Gly,seq:tcc)
     gene            2004..2078
                     /locus_tag="WIE35_001924"
     tRNA            2004..2078
                     /locus_tag="WIE35_001924"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2036..2038,aa:Thr,seq:ggt)
BASE COUNT          634 a          448 c          521 g          521 t
ORIGIN      
        1 attaaataac ttagggcgat gaggatgaca ggtgtcaaga aaacaatagt tacatcccga
       61 tagctataaa agaaaactgc cccattttga atgtggaaat gtgcacagaa tccaatatag
      121 ctaaggataa tagttaatat aataaaaaaa gaccaccaag ggtgaagaga taggaattcc
      181 atgttttccc tttttttgta aaaaggaaaa aatcttatct aatagttaat tgcctaacca
      241 gccagaagaa gtttaaaatc tatcccaata attcaaccat ctatacagaa agttcagctt
      301 atggaaaccc acgaaaaaat ccgcctgatg cgcgaattga ataaatggtc ccaggaggat
      361 atggcggaaa agctggcgat gtcggcaggc gggtatgcca aaatcgaacg aggcgaaacg
      421 cagttgaata tcccgcgttt ggagcagttg gctcagattt tcaaaatcga tatgtgggac
      481 ttgctcaaat cgggcggtgg tgggatggtg tttcagatta atgaaggtga tagtggtggc
      541 gatattgcgt tgtatgcgtc gggtgatgtt tcgatgaaaa tagaattttt aaaaatggag
      601 ttgaaacact gcaaagaaat gttggaacaa aaagacaaag aaatcgagct gctccgcaag
      661 ctgaccgaaa ccgtttaaac agatatgccg tctgaaaaaa gttttcagac ggcatattct
      721 ttgacaggtc ttgtataata ccgtttgaac ttacaggttt ttgattatgg cggcaggcaa
      781 acataccaaa cacagcaacc gggtacgcat tatcggcggg caatgccggg gcaggaaatt
      841 gagtttcaca tccgccgacg gactgcgtcc gacacccgac agcgtgcgtg aaaagctgtt
      901 taactggctg ggacaggatt tgacgggtaa aacggttttg gatctcttcg gaggcagcgg
      961 cgcactcggt atagaagccg cttcgcgcaa cgccaaacgc gtgctgattt cggataacaa
     1021 ccgccaaacc gtgcagacct tgcagaaaaa cagtcgcgaa ctgggtttgg ggcaggtgca
     1081 aatcgtcttt tcagacggca tcgcatattt gaagaccgta tccgaacagt ttgatgttgt
     1141 ctttctcgac ccgccgtttg catggcagga ctggcaaatc ctgttcgatg ccttgaagcc
     1201 gtgcctgaac ccccgggcat tcgtctatct cgaggcgggt acgctgccga atattcccga
     1261 ttggctgacg gaatatagag aagggaaatc ggggcagagt acattcgaat taagggtttt
     1321 ccaagtggct gaataatatg cgctttgata atcatttccg agttgtaaac attcgtttgc
     1381 aaccgtccgg ttcaaaaaaa ccttgtgcta taatccgcgc ccgcccggtt ttgataattt
     1441 agtggaaaag gaaaagaaat gtcgcttttt attaccgacg agtgcatcaa ctgcgacgta
     1501 tgcgaacccg aatgccccaa tgatgccatt tcccaaggcg aggaaattta cgaaatcaac
     1561 cccaacctct gcacgcagtg cgtcggacac tacgatgagc cgcagtgcca gcaggtttgc
     1621 ccggtggact gcatcctgat tgacgaagaa catcccgaaa cccatgacga gttgatggcg
     1681 aaatacgaaa agattatcca gtttaaataa attcttttta aaacatcaaa ttatgtctgt
     1741 tttgaaataa aatcaaaaaa aaacttgacg gaaaagcaag ccgctaataa actaacgttc
     1801 tcttttggag ggattcccga gcggtcaaag ggggcagact gtaaatctgt tgcgaaagct
     1861 tcgaaggttc gaatccttct ccctccacca aaattcttac ttggggcagt agcgagtaat
     1921 gcgggtgtag ctcaatggta gagcagaagc cttccaagct tacggtgagg gttcgattcc
     1981 cttcacccgc tccaaacaat taggcccatg tagctcaggg gtagagcact cccttggtaa
     2041 gggagaggtc ggcagttcaa atctgcccat gggcaccatc cctcgattat tcatttcttt
     2101 aaggcttaga tatataggat attg
//
LOCUS       DAWHXZ010000247         2117 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.247, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000247 DAWHXZ010000000
VERSION     DAWHXZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.247"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..346
                     /locus_tag="WIE35_001925"
     CDS             <1..346
                     /locus_tag="WIE35_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ4154797.1"
                     /translation="VNYISNQQLTNFTIKDEFKDALDINFAFYYFFIIAEQSKKLINT
                     SSLPIISMKELKKLKIPIPPLPEQEKIAAILDKFDILTHSISEGLPHEIALRRKQYEY
                     YREQLLAFPKAT"
     gene            425..>2117
                     /locus_tag="WIE35_001926"
     CDS             425..>2117
                     /locus_tag="WIE35_001926"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HEZ4154798.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSKPESGGYVWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSTDSVNGSESTAGLKRNLEKDDNKIIVTTIQKLNNLMKS
                     EDNLPVYHQRVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALL
                     HPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGS"
BASE COUNT          634 a          526 c          507 g          450 t
ORIGIN      
        1 agttaattat attagtaacc aacaactgac caattttaca attaaagatg aatttaagga
       61 tgctttagat ataaatttcg ctttctatta ttttttcatt attgcggagc agagcaaaaa
      121 acttattaat acttcaagtt tacctattat tagcatgaaa gaattaaaga aattaaaaat
      181 ccccatccca ccactccccg aacaggaaaa aattgccgcc atcctagaca aattcgatat
      241 cctgacccac tctatcagcg aaggcctgcc gcacgaaatc gccctgcgcc gcaaacaata
      301 cgaatattac cgcgaacagc tgcttgcctt ccctaaggct acctgaaaaa gcagcctgca
      361 cattttcagg cagcccaatc cgaacatcaa accgtttaaa acccgatact gcaaaggacg
      421 acccatgaac ctcgaaacca aacccatcgc cgaaacgccg aatttcatcg tgctcgacca
      481 atatgaaaaa atcgaacagt cgggcagcta ccaatcggaa aaccggttgg aagcggagtt
      541 aatcgccgat ttgcagaatc agggttacga ataccgcaag gatttgaaca gccaaagcag
      601 gctgctggaa aacctgcgcg cgcagttgca gcggctgaac gatgtggcgt tttcagacgg
      661 cgaatgggcg cggtttttga cggaatatct ggacagaccg tctgaaaata ttaccgataa
      721 aacccgcaaa atccacgacg accatattta cgatttcgct tttgatgacg gccgtctgaa
      781 aaacatttat ctgctggaca agaaaaacct tgcccgcaac catgtgcagc ttatcaacca
      841 gtttgagcag acgggcacgc atgcaaaccg ttatgacgtt accgtgttgg taaacggtct
      901 gccgctggtg cagattgaat tgaaaaagcg cggtgtggcg gtgcgcgaag cattcaatca
      961 ggtgcaccgt tacagcaaag agagcttcaa cagcgaaaat tcgctgttca aattcctgca
     1021 aatcttcgtg atttccaacg gcacggacac gcgctatttc gccaacacca ccaagcgcga
     1081 caaaaacagc ttcgatttca cgatgaattg ggcgcggtcg gacaatcatc cgattaagga
     1141 tttgaaagac tttaccgcca cgttcctgca aaaaagcgta ttgctgagcg ttttgctgca
     1201 ttacagcgtg ttcgatgcga atgatacgct gctgattatg cggccgtacc agattgccgc
     1261 cgccgaacgc attctgtgga aaatcaacag ctcggcgcag gcgaagaatt ggagcaagcc
     1321 ggagagcggc ggctatgttt ggcacaccac gggcagcggc aaaacgctga ccagctttaa
     1381 ggcggcgcgt ctggcgacgg aatcggcatt tatcgacaag gttttcttcg tggtggacag
     1441 gaaggatttg gactatcaga cgatgaagga ataccaacgt ttttcgaccg acagcgtgaa
     1501 cggttcggaa agcacggcgg gcttgaaacg caatttggaa aaagacgaca acaaaatcat
     1561 cgttaccacc atccaaaagc tgaacaacct gatgaaaagt gaagataatc tgccggttta
     1621 ccatcagcgg gttgtcttta ttttcgacga atgccaccgc tcgcaattcg gcgaagcgca
     1681 aaaaaacctg aaaaagaaat ttaaaaaatt ctgccagttc ggttttaccg gcacgccgat
     1741 ttttcccgaa aacgctttgg gcgcggaaac cacggcgggc gtgttcgggc gggagctgca
     1801 ttcttatgtg attaccgatg ccatccgcga tgaaaaagta ttgaaattca aagtggatta
     1861 caacgatgtg cgcccgcagt tcaaagccgt ggaagcggaa caggacgaga agaaactgag
     1921 tgccgccgaa aaccgccaag ccctgctgca ccccgaacgc atccgcgaaa tcacgcaata
     1981 tatcctgaac cagttcaggc agaaaacgca ccggctgaat gcgggcggca aaggctttaa
     2041 cgcgatgttt gccgtcagca gcgtggatgc ggcgaagtgc tattacgaag cgttcaaaac
     2101 gcaacaggca ggcagcc
//
LOCUS       DAWHXZ010000248         2095 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.248, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000248 DAWHXZ010000000
VERSION     DAWHXZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.248"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            136..2016
                     /locus_tag="WIE35_001927"
     CDS             136..2016
                     /locus_tag="WIE35_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEZ4154799.1"
                     /translation="MNIFYEESGQFKIAAIVQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLALPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAYRPAVSIYFDVDSG
                     FNAGEPTCKIEAVNIAKNLRIQAIEPHFNAETGLDEAGEMMFVHHQDLIWFHQFAVAL
                     QKARGKYEPNRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVRHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          569 a          636 c          503 g          387 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagacgcgcc aaccctctcc
       61 caaccttttt tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac
      121 agaaaaatcc gaaaaatgaa tattttttac gaagagtccg gccaattcaa aatcgccgcc
      181 atcgtccaaa aaaacgacgc cacctaccaa gtcgataccc cacacggcaa acgcaccaaa
      241 gtgaaggcga acaacgtctt tgccgagttt gacggcgata tggcggcgtt tttggaaaac
      301 gcgcaggcac aggcggcgga catcgacacc gatttattgt gggaagtatg cggcgaagag
      361 gaatttaccg ccgaagccat cgccgaagaa tattacggcc atgcgccgac caaaaccgag
      421 ctggcggcaa ctttgattgc gctttacgcc gcgccgatgt atttctacaa aaaagccaaa
      481 ggcgtgttca aagccgcgcc cgaagaaact ttaaaacaag cacttgccgc catcgaacgc
      541 aaaaaacagc aagacgcgca aatcgacgct tgggcggaag ccttgaaacg cggcgagatg
      601 ccgtctgaaa tcgcggcgga tttgaaaacc atcctgcacg cgcccgacaa gcagtcgctg
      661 acctacaaag cctttaccaa agccgccgac gcgctgaaaa cctctgccta cgaattggcg
      721 aaaaaaacgg gcggcattac gtccattccc caatacctgc aagacggatt tgaaatcaaa
      781 tacttcccca aaggaacagg cttccccgac cttgcccttc ctgaaatgcc cgacctgccc
      841 aaggctgacg ttaccgcctt ttccattgac gacgaatcga ccaccgaagt ggacgacgct
      901 ttaagcctga ccgacttgga caacggcacg aagcgcgtcg gcatccacat cgccgcgccg
      961 tcgcttgcca ttaaacaagg cgacaaaatg gaaaaaaaca tcatggaacg cttgagcacg
     1021 gtttatttcc ccggcggcaa aatcacgatg ctgcccgaaa actggatagc cgcgttcagc
     1081 cttgatgcag gcgcgtaccg tcctgccgtc agcatttatt ttgatgtgga cagcgggttc
     1141 aacgccggcg aaccgacctg caaaatcgaa gcggtcaaca tcgccaaaaa cctgcgcatc
     1201 caagccatcg agccgcattt caacgccgaa accggcttgg acgaagcagg cgaaatgatg
     1261 ttcgtccacc atcaagacct gatttggttc caccaatttg ccgtcgccct gcaaaaagca
     1321 cgcggcaaat acgagcccaa ccgtgcgccg caatacgatt acagcatcga attggataaa
     1381 gaagacaacg tatccgtcgt ccgccgcgaa cgcggttcgc ccatcgatac gctggtcagc
     1441 gagatgatga ttcttgccaa cagcacttgg gcgcaaatgc tccatgacaa cgacctgccc
     1501 ggcctcttcc gcgtccaacc caccggaaaa gtacgcatga gcaccaaatc cgagccgcat
     1561 atcggcatgg gcgtgcggca ttacggctgg tttacctcgc cgctgcgccg cgccgccgac
     1621 tacatcaacc aaaagcagct gatcagcctg atagacgaca ctgccgagcc gctgtatcaa
     1681 aacagcgatg ccgagctttt cgccgcactg cgcgactttg atgccgccta taccgcctac
     1741 gccgacttcc aacgccaaat ggaagcctac tggagcctgg tttacctgca acagcaaggc
     1801 acgagcgagc tgacggcgac gattctgaaa gaagacctcg tccgcatcga aggtctgccg
     1861 ctagtaacac gcgcgaccgg tattccgttt gacgcgctgc ccaaatcgca ggcattgttc
     1921 aaaatcaccg aattagatgc cgagaagcag tttgtctcgc tcaactacat caaggcagtc
     1981 gcacccgcgg gtacaacggc aggcaatgcc gtctgaagcc cgatacggca aactccggca
     2041 aaaacagaaa cctgaatctc atcattccca cagtcgggaa tccggttttt tgggt
//
LOCUS       DAWHXZ010000249         2053 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.249, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000249 DAWHXZ010000000
VERSION     DAWHXZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.249"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..751
                     /locus_tag="WIE35_001928"
     CDS             <1..751
                     /locus_tag="WIE35_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ4154800.1"
                     /translation="ARPDSVIDGVSVYQSRLDMGVSLAEKIKRELPVDDIDVVMPIPD
                     TSRPSAMELAVHLDKPYREGLIKNRYIGRTFIMPGQATRKKSVRQKLSPMETEFAGKS
                     VLLVDDSIVRGTTSREIVEMVRAAGARKVYIASAAPEVRYPNVYGIDMPTREELIANG
                     RSAAEIAAEIGADGIVFQNLSDLEAVVKALNPKIESFDSSCFNGIYQTGDIDDAYLDR
                     LSAEKSGCGGLKIHPSRMEHSISISDAGDEE"
     gene            851..1342
                     /gene="greB"
                     /locus_tag="WIE35_001929"
     CDS             851..1342
                     /gene="greB"
                     /locus_tag="WIE35_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ4154801.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            1401..2027
                     /locus_tag="WIE35_001930"
     CDS             1401..2027
                     /locus_tag="WIE35_001930"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ4154802.1"
                     /translation="MRKIRTKICGITTLEDAAAAAAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT          504 a          631 c          549 g          369 t
ORIGIN      
        1 tgcccgcccc gactccgtaa tcgacggcgt atcggtttac caatcgcgct tggatatggg
       61 cgtgtccttg gcggaaaaaa tcaaacgcga gctgcccgtg gacgacatcg atgtcgtgat
      121 gcccattccc gacaccagcc gccccagcgc gatggagctt gccgtccacc tcgacaaacc
      181 ctaccgcgag ggcttgatta aaaaccgcta tatcggccgc acctttatta tgcccggcca
      241 ggcgacgcgc aaaaaatccg tgcgccaaaa actcagcccg atggaaaccg agtttgcagg
      301 caaaagcgtg ttgctggtgg acgactccat cgtgcgcggg acgaccagcc gcgaaatcgt
      361 cgaaatggta cgcgcggcgg gcgcgcgcaa agtctatatc gcctccgccg cccccgaagt
      421 gcgctatccc aacgtatacg gcatcgatat gcccacgcgc gaagagttga ttgccaacgg
      481 gcgcagcgcg gcggaaatcg ccgccgaaat cggcgcggac ggcatcgtgt tccaaaattt
      541 gagcgatttg gaagccgtcg tcaaagcact caatccgaaa atcgaatcct tcgattcgtc
      601 ctgtttcaac ggcatttatc agaccggcga catcgatgat gcctacctcg accgcctgtc
      661 tgccgagaaa tccggctgcg gcggcctgaa aatccacccg agtaggatgg aacacagcat
      721 cagcatcagc gatgcgggcg acgaagaata aggcttccca aaccgcgatg ccgtctgaaa
      781 tccgaacgcc gggttttcag acggcatttc ccatatcggc atcacatttc ccatttcatc
      841 cgcacgcaat atgagtaccg aaaccaaaaa ctacattacg cccgccggat ggcaggcatt
      901 gaaagacgag ctttatcaac tggttaataa agaacgcccc gaaatcgtcc aaatcgtcaa
      961 ctgggcggca ggcaacggcg accgcagcga aaacggcgac tatctttacg gcaaacgccg
     1021 tatgcgcgaa atcgaccgcc gcatccgttt cctgaccaag cgtttggagt ccgccgtcgt
     1081 cgtcgatccc gaattgcgcg aagcgaccga ccaagtattt ttcggcgcga cggtcgggct
     1141 gttgcgcggc gacgggcgcg agcaaaccgt caaaattgtc ggcatcgatg aaatcgatac
     1201 cgcgcaaaac aaaatttctt ggatttcgcc gttggcgcgt tgcctgatta aagcgcgcga
     1261 aggcgacgaa gtggttttga acacgccgga agggcgtgag gaaattgaaa tcctttctgt
     1321 cgaatacatc aaaatcgact gatcgggaat gccgtctgaa acggcacaaa cgcatcgttt
     1381 cccaaataaa ggaataccga atgaggaaaa tccgcaccaa aatctgcggc atcaccacac
     1441 tggaagacgc agcggccgcc gcagcggcag gtgcggatgc cgtcgggctg gtctttttcc
     1501 aaggcagcag ccgggccgtc gatattgccc gcgccaaaaa aatcaccgcc gcactgccgc
     1561 cgtttgtcag cgttgtcgcc cttttcgtca acgaaagcgc gcaaaacatc cgccgcatcc
     1621 ttgccgaagt gccgatacac atcatccaat tccacggcga cgaagacgac gcattctgcc
     1681 gccagttcga ccgcccctat atcaaggcca ttcgtgttca gacggcatca gacatccgaa
     1741 acgccgccga ccgcttcccc gacgctcagg cactgctgtt cgatgcctac cacccttcgg
     1801 aatacggcgg caccggacac cgcttcgact ggacgctgct ggcggaatat tcgggcaaac
     1861 cgtgggtgct tgccggcggg ctgacccctg aaaacgtcgg cgaagccgtc cgcatcaccg
     1921 gagcggaagc ggtcgatgta tccggcggcg tggaagcgtc taaaggcaaa aaagacccag
     1981 ccaaagttgc cgcctttatc gcaaccgcca accgcctatc ccgttaaagc aacaaaaatt
     2041 gccgccggaa tga
//
LOCUS       DAWHXZ010000250         2003 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.250, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000250 DAWHXZ010000000
VERSION     DAWHXZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.250"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(144..941)
                     /locus_tag="WIE35_001931"
     CDS             complement(144..941)
                     /locus_tag="WIE35_001931"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ4154803.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            1096..>2003
                     /locus_tag="WIE35_001932"
     CDS             1096..>2003
                     /locus_tag="WIE35_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ4154804.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKANIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDSEKV"
BASE COUNT          500 a          567 c          493 g          443 t
ORIGIN      
        1 tctgataaat caccgtaacg ttaaatttcc agattcccat tttcgtgaac ataacggtgt
       61 gaacattagc aaaaattcaa aaaaccaaac ttcctcattc gtgtgaatga tggtactgat
      121 attttctatt tcagttcgca aaattaatct ttcaacacat cttgcacagc cgcctgaatc
      181 ttagccgccg ctgcctcgat ttgccgttgt tcggcagatg ggtttgccgc tgtttcaacc
      241 ggagcagttt gtaaagcagg ctcagactca ggctcggatt cgatttgcgg cgcagtcagc
      301 ggcaaacgcg ccaatacagt gctaacgcca gttactttat cgccaatcgc cacttgcgcc
      361 tgcgcatcga caggcagata catatccacg cgcgaaccga agcggataaa gccataacgt
      421 tcgccgcggg acagtttcgc acctgcttgg gtgtagcaca aaatacggcg cgccaccaaa
      481 ccggccactt gaacaaaagt aatttcacga ccggaagccg tagtcgccaa caccgcatta
      541 cgttcgtttt ccgtgctggc tttgtccaaa tccgcattca cgaatttgcc tttgttatag
      601 accactttcg ttaccgtaca atcggcaggc gatttttgcg aatgcacgtt gaacacgttc
      661 ataaaaatac tgattttcaa agcatcgaca tcacgatacg gatcgcgtgc gcgctcgacc
      721 accacgatac ggccgtcaac cggactcaac actgcttcag gattttgcgg aatctcacgc
      781 gcagggtcgc ggaaaaactg cagggcaaat acggtaaaca cccaaaacgg caaagaccac
      841 cagccgcaac acatcgacac cagcaggctc aaagccaaac cgccgccaat aatcggccag
      901 ccctcacggg cgataatcgg gtgggggtaa agacggttca tttgtcatcc ttttgttcaa
      961 gcgggttaaa attgccgaca ttatagcgga atctgccgta ctccgccgac aaatgccgtc
     1021 tgaacgcggc ttcagacggc atccgagaaa gtgctacaat accagttttc ctttattttt
     1081 caggcggcat ccattatgtg caaccatcat ccccaacatt cgcacgacaa cgacaccatc
     1141 cgcatacgcg gcgcgcgcac gcataatttg aaaaacgtcg atttagacat tccgcgccac
     1201 aagctggtgg tggtaacagg attgtcaggc agcggcaaat catcgctggc gttcgacacg
     1261 ctgtatgccg aaggacagcg gcgttatgtc gagagcctgt ccgcctatgc gcggcagttt
     1321 ttgcagatga tggacaaacc cgatgtcgat ttgatcgagg gtctgtcgcc cgcgatttcc
     1381 atcgagcaga aatccaccag ccacaatccg cgttccaccg tcggcacggt tacggaaatc
     1441 cacgattacc tgcgcctttt gtacgcacgc gtcggcacgc cgtattgccc cgaacacaat
     1501 ctgccgctat ccagccaaac cgtatctcag atggtcgatg ccgtattaaa actgccggaa
     1561 gacacgcgcg tgatgattct tgcgccggca gttcgtgagc gtaagggcga gtttgtcgac
     1621 ttctttgctg acttgcaggc gcaaggcttt gcccgtgtgc gcgtggacgg cgaggtctat
     1681 cagcttgacg aagtgccgaa gctggaaaaa aacatcaaac acaatatcga cgtggtcatc
     1741 gaccgcgtga aagtgaaggc gaacatcaag cagcggctgg cggaaagttt tgaaaccgcg
     1801 ctgcgccacg gcaacgagcg cgcgctggcg atggaaatgg acagcggcga agagcattgg
     1861 ttttccgcgc gtttcgcctg ccccgtatgt tcgtacagcc tgcccgaatt ggagccgcgc
     1921 ctcttttcgt tcaacaaccc gatgggctcc tgcccgactt gcgacggctt gggcaacacc
     1981 aatttcttcg actccgaaaa agt
//
LOCUS       DAWHXZ010000251         2000 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.251, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000251 DAWHXZ010000000
VERSION     DAWHXZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.251"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            300..1757
                     /locus_tag="WIE35_001933"
     CDS             300..1757
                     /locus_tag="WIE35_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ4154805.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPIVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKCYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          456 a          590 c          458 g          496 t
ORIGIN      
        1 agtacggcaa ggcgaggcaa cgccgtactg gtttaaattt aatccactat aaaaaaccgc
       61 cctatcggat aagcccttaa cagaaaaggc tttacccgcg ccgtatcgga acacatcctc
      121 taaaatacaa tccgttgaat tgaaaaaaat ataaaaacat ccgcccgcga aaaacggcag
      181 cgcgtcgttt gacaaagaat gaaaatatcg gttaaaaacc gattttcata caaaaaacac
      241 cgctgccgtc cgcatccgtt tcagacggta ttgagagaaa atcttttagg agaaccttta
      301 tgtcccggca tcccgccccc accggagaaa aaacattctt cggccacccc ttccagcttt
      361 ccaccctctt ccatatcgaa ttgtgggaac gtttttcatt ttacggaatg cagggcatcc
      421 tgctgattta cctctactac accgccgaca aaggcggctt gggcatagac aaaaccctcg
      481 ccggcggcat tgtcggcgca tacagcggca gcgtgtacct gtccaccatt ttgggggcgt
      541 ggtttgccga ccgagtatgg ggtgcggaaa aaaccctctt cctctcgggc atcgtcgtga
      601 tgctcggaca catcgtcctt gccgccgccc cgggcctgta cggcctttta atcgggctga
      661 tattcatcgc attgggcagc ggcggcgtga aatctacggc cagttctatg gtgggcgcat
      721 tatacgaaca ggacgaaatg cggccgctgc gcgatgcggg attttccatt ttctacatcg
      781 ccatcaatat cggcggcttc ctaggcccgc tgctgaccgg cctgctgcag gaaaacatcg
      841 gtttccatta tggtttcggc gcggcggcgg tcggtatggc attcggcttg tggcgttatt
      901 ccttgggacg taaaaacctg ccccatccca tcgtccccca tccgctttca aaaggacagg
      961 gcaaaactgc ggccgccgtc ggcattgctc tcatcgccgc acttgcaacc gccatcaaaa
     1021 ccgggcttgt caacctcgac aatttctccg gcatcctctt atctaccgtc atccttgccg
     1081 tcattgccta ttttgcccgc ctgctgacta atccccgcgt cagttccgac aacaaacggc
     1141 acatcatcgc ctacatcccg cttttcctga ccatctgtat gttttgggcc gtctggtttc
     1201 agatttacac cgtagcaacc gtctatttcg acgaaaccgt caaccgcacc atcggttcgt
     1261 ttaccgtgcc cgtcgcctgg aaagattcca tgcaaagcct gtgggtcatc ctgttttccg
     1321 gactgatggc ggcaatgtgg acaaaaatgg ggcgcaaaca gcccaaaacc ccgctgaaat
     1381 tcgctatggc ggtatttgtt accggcgcgt cgtttttggg attcgtcccc tttatttccg
     1441 ccggcacgcc gatgcctatt gcggttttcg cactgattgt ccttgccatc acgataggcg
     1501 aactgatgat ttccccgatt gcgctgtcca tctccaccaa aatcgcaccg cctttattca
     1561 aaacccaaat ggtcgccctt aatttccttg ccttttcatt aggcttcact ttgggcggcg
     1621 tattgtttga aaaatgctat caggcgggcg acgaaatcgg cttctatcgg ctgctgttct
     1681 acatcggcgc agccacaggc ttcctgctgc tcctgctcgt ccccaaattg aacaaaatgc
     1741 tcgaaggcac agactaagtc ccgccccgat gccgtctgaa cccttcagac ggcatttttc
     1801 cgcataatga aaccaaaccg tttccacccg acaggacagg ctccccccaa ccggaaggca
     1861 gcctgccgat tgtcatttaa atatctgact gattccgtct gtttgcaagg gaaatagccg
     1921 tttatttccg cttgggcgga aaccggttct attgagtaaa aggcatttta tccgactaaa
     1981 ttagtgctgc atcaacggaa
//
LOCUS       DAWHXZ010000252         1995 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.252, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000252 DAWHXZ010000000
VERSION     DAWHXZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.252"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            43..1260
                     /locus_tag="WIE35_001934"
     CDS             43..1260
                     /locus_tag="WIE35_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ4154806.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTSIAIIGLANIAGCIF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLTMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            complement(1463..1603)
                     /locus_tag="WIE35_001935"
     CDS             complement(1463..1603)
                     /locus_tag="WIE35_001935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154807.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPARAGAASDGI
                     LS"
     gene            1616..>1995
                     /locus_tag="WIE35_001936"
     CDS             1616..>1995
                     /locus_tag="WIE35_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEZ4154808.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEG"
BASE COUNT          437 a          658 c          459 g          441 t
ORIGIN      
        1 cactataaaa tccgcaaact taaccgactc aaaatacaca ttatgacgca caccgcatcg
       61 aaaacaccca aattttggct gaccatcacc gccgccgcat tcatcctgct gattaccatc
      121 ggcatgagga tgacgctcgg actgttcgtc caacccgtcg tcaacaccac cgaattgagc
      181 attgcccaat tcagcctcat catcgccgtt ttccaactga tgtggggcat atcgcaacca
      241 ttgtccggcg cgcttgccga ccgtttcggc gcgttcaggg tattaagcgg cggcgccgtc
      301 ctgctcgtct gcgcctgcct gattgccccc aacatcccga cttattgggg gctgatgatt
      361 gccgtcgggc tgctgctcgc attcggcacc ggttccggcg gtttctccat cattatgggg
      421 caggtcgccg cccaagtccc cgcacacaaa cgcggcttgg catccggact ggtcaacgcc
      481 ggcggttcgg caggacaatt cctgttcgca ccgctggttc aaggactcgt cgtcctgccc
      541 gaagtcggct ggacgggcac attttacgtt tggggcgcaa tcgccctgct gattctgcct
      601 gtctcatggt ggcttgccgg cggcaacaac gccgcccaca cccaacacgc ccaagccaca
      661 cacggacaaa gcctcggaga agcagtcaaa accgccttca aaaccccaag ctacatcctg
      721 ctgcacctaa gcttttttgc ctgcggcttc cacatcgcct ttctcgtaac ccacctaccc
      781 acggaagtcg ccctgtgcgg actgcccgcc accgtcgcct cgacatccat cgccatcatc
      841 ggactggcaa acatcgccgg ctgcattttt tccggctggt gcacaagccg cgttcggggc
      901 aaatacgtcc tgttcggact gtacgcctcg cgcgccgcga tgatactcat ctacattttt
      961 tcgcccaaga ccgacctcaa cttctacatt ttcgccgccg cactcggatt tacctggctc
     1021 gccaccgtca cgccgaccgc ctccattacc ggcaaactct tcggcacacg ctacctcgcc
     1081 accctgttcg gactgacgat gctcagccac caaatcggcg gattcctcgg ctcgtacatc
     1141 ggcggcatcg tgattacaca attcggcgac tacggctgga tgtggtacgc cgacgcactg
     1201 ctggcgggaa ccgccgccct cctcaacctc cccatccgcg aaccccgaac cgccgcatga
     1261 aagaatgccg tctgaaaacc ctcagacggc attttccaaa caccccgccc gtttccggac
     1321 tgcacgacac gcaaatcagc cttatcaaac cgcaaacaac cgccgcgatt aaaccgccat
     1381 tcaaataagt ccgttttccc ttcgggtgta aaatcatcac aaaacgcgat actgtatatc
     1441 gccgggcgtt atgcagcgga tttcaggata aaatgccgtc tgaagccgcc cctgcccggg
     1501 cgggcgaagc gtcggatgcg gtgcgcttgg caaaggtttg cccgccgcgc cccgtatccg
     1561 gcatttcatc aatgcagacc gcatttccaa tcctataatt caaggtgtcc gccttatgaa
     1621 aatcgttttt atcacaacag tcgcatccag catttacggt ttccgcgccc ccgtcattaa
     1681 aaaattaatc ggcaaaaacc atcaggtgta tgcctttgta tcggagtttt ccgataatga
     1741 attggatatt atcagggaaa tgggggtaac acccgttacc taccgttcaa accgcagcgg
     1801 gctgaacccg ttttccgata taaaatccac cttcctcatc tttaaagaac tcaaaaaaat
     1861 atcgccggat ttggttttcc cttatttcgc aaaacccgtg attttcggca cttttgccgc
     1921 aaaactggca ggcgtgccca gaatcgtcgg gatgctggaa ggtttgggat tcgcatttac
     1981 cccgcagccg gaagg
//
LOCUS       DAWHXZ010000253         1963 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.253, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000253 DAWHXZ010000000
VERSION     DAWHXZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.253"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            8..1861
                     /gene="uvrC"
                     /locus_tag="WIE35_001937"
     CDS             8..1861
                     /gene="uvrC"
                     /locus_tag="WIE35_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ4154809.1"
                     /translation="MNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEGGKVLYVGKAVN
                     LKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNFIKALSPKYNILF
                     RDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQVLQKVFMLRTC
                     EDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYHDSVREAATFLNGKTDELTRTLQHK
                     MQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLALAVSDGLVCVH
                     WVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDIIISNFSVPDAL
                     KEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQQHRIDELAKIL
                     GMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITTAKPGDDYAAMR
                     EVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGLHIPLVGIAKGP
                     ERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRKKRDKARVTSSL
                     SDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIYNHLH"
BASE COUNT          550 a          577 c          459 g          377 t
ORIGIN      
        1 gattgatatg aatacagaaa accgttctcc ggaacaattc gacatcccgc tcttcctcaa
       61 aaacctgccc aagctgccgg gcgtgtaccg tttttttgac gaaggcggca aagtcttata
      121 cgtcggcaaa gcggtcaacc tcaagcggcg cgtgtccggc tatttccaaa aaaacgacca
      181 ttcgccgcgc atcgcattga tggtaaaaca ggtgcatcat atcgaaacca ccatcacgcg
      241 ttccgaagcc gaagcactga ttctcgaaaa caacttcatt aaagctctgt cgccgaaata
      301 caatattctt ttccgcgatg acaaaagcta tccttatttg atgctcagtg gccatcaata
      361 tccgcaaatg gcgtattacc gcggcacact gaaaaagcca aatcaatatt tcggcccgta
      421 tcccaacagc aacgccgtgc gcgacagtat tcaagtgttg caaaaagtct ttatgctgcg
      481 tacctgcgaa gacagtgtat tcgagcaccg cgaccgtcct tgtctgcttt accaaatcaa
      541 acgctgtacc gcaccttgtg tcggtcatat cagcgaagaa gattatcacg acagcgtgcg
      601 tgaagcagca actttcctca atggcaaaac cgacgaactg acgcgtaccc tgcaacacaa
      661 aatgcaaacc gccgccgcta atctgcaatt tgaagaagcc gcccgctacc gcgaccaaat
      721 ccaagcactc ggcattatgc agagcaacca gttcatcgac agcaaaaacc cgaacaaccc
      781 caacgacatc gacctgctcg cgctcgccgt ttcagacggc ctcgtctgcg tacactgggt
      841 cagcatacgc ggcggacggc atgtaggcga taaaagcttt ttccccgaca ccaaaaacga
      901 tcccgagcca aacggacaag attacgccga agccttcgtc gcccaacatt atttgggcaa
      961 aagcaaaccc gacatcatta ttagcaactt ttccgttccc gatgcgctga aagaggcttt
     1021 ggaaggcgaa cacggcaagc agatgcaatt tgtcaccaaa accataggcg aacgcaaagt
     1081 ctggttgaaa atggcggaac aaaacgcaca aatggcgatt gcacaacgcc gcctgcaaca
     1141 aagcagccag caacaccgca ttgatgaact ggcaaaaatc ctcggcatgg attcagacgg
     1201 cctcaaccgc cttgaatgtt tcgacatcag ccacacacaa ggcgaagcca ctattgcgtc
     1261 ctgcgttgtg tacgatgagc aaaacatcca gccttcgcaa taccgccgct acaacatcac
     1321 gaccgccaaa cccggcgacg actacgccgc catgcgcgaa gtgttgacgc gccgttacgg
     1381 caaaatgcag gaggccgaag ccaacggcga gaccgtcaaa tggccggatg ccgtgttgat
     1441 tgacggcggc aaagggcaaa tcggcgtagc cgtatcggta tgggaagaac tcgggctgca
     1501 catccctttg gtcggcattg ccaaaggccc ggagcgcaaa gccggtatgg aggagctcat
     1561 actgcctttt accggcgaag tcttccgcct gccgcccaac agcccggcct tgcatctatt
     1621 gcaaaccgta cgcgatgaat cgcaccgttt cgccattacc ggtcaccgca aaaaacgcga
     1681 caaagcccgc gttacctcct ccttaagcga catccccggc gtaggcagca aacgccgcca
     1741 agccctgctc acccgcttcg gcggtctgcg cggcgtgatt gccgccagcc gcgaggactt
     1801 ggaaaaagtg gaaggcatca gcaaggcatt ggcggaaacg atttacaatc atctgcatta
     1861 gcatgctgtc aaagacaaaa tccgtctgta aaaaatatga tacagcaggt cggtataccg
     1921 atatatagtg gattaaattt aaaccagtac ggcgttgcct cgc
//
LOCUS       DAWHXZ010000254         1949 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.254, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000254 DAWHXZ010000000
VERSION     DAWHXZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.254"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1842
                     /gene="pepN"
                     /locus_tag="WIE35_001938"
     CDS             <1..1842
                     /gene="pepN"
                     /locus_tag="WIE35_001938"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ4154810.1"
                     /translation="NMGAMENKGLNIFNTKFVLADSRTATDTDFEGIESVVGHEYFHN
                     WTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRAVRRIENIRLLRQHQFPEDAGPT
                     AHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLGEEGFQKGMKLYFQRHDGQAVTC
                     DDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRLKNNSFELTIKQTVPPTPDMADK
                     QPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLLTEAEQIFLLEGVTEAVVPSLLRG
                     FSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTLYRRAVAANLAALSDGIELPKHE
                     KLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGAENIDPLRYHQAREALLDTLAVR
                     FLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVCRAFVLRADPAHIETVAEKYGEM
                     AQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDDALVMDKYFALIGSSRRSDTLQQ
                     VQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAEDGSGYRFIADKVIEIDRFNPQVA
                     ARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSKDVGEIVGKILD"
BASE COUNT          470 a          612 c          518 g          349 t
ORIGIN      
        1 aatatgggcg cgatggaaaa caaggggctg aacattttta ataccaagtt cgtccttgcc
       61 gacagccgta ccgccaccga taccgatttt gaaggcatcg aatccgtggt cggacacgag
      121 tatttccaca actggacagg caaccgcgtt acctgccgcg attggttcca gctttcgctg
      181 aaagaagggt tgaccgtgtt ccgcgaccaa gaattttccg gcgaccgcgc cagccgcgcc
      241 gtgcgccgca tcgagaacat ccgcctgctg cgccagcacc agttccccga agacgcaggc
      301 ccgaccgccc atccggtgcg ccccgccagc tatgaggaga tgaacaattt ctacaccatg
      361 accgtttatg aaaaaggcgc ggaagtggtg cggatgtatc acaccttgct cggcgaagag
      421 ggcttccaaa aaggcatgaa gctctatttc caacgacacg acgggcaggc cgtgacctgc
      481 gacgatttcc gcgcggcgat ggcggacgcg aacggcatca atctcgacca gttcgccttg
      541 tggtacagcc aggcgggtac gcccgtcttg gaagcggaag gtcgtctgaa aaacaatagt
      601 ttcgagttaa ccatcaaaca aaccgtgccg cccacgcccg atatggcgga caagcagccg
      661 atgatgattc ccgtcaaagt cgggctgctg aaccgcaacg gcgaagcggt ggcattcgat
      721 tatcagggca aacgcgcgac cgaagccgta ttgctgctga ccgaagccga acagatcttc
      781 ctgctcgaag gcgtaaccga agccgtcgtt ccctcgctgc tgcgcgggtt cagcgcgccg
      841 gtgcatctga actatccgta cagcgacgac gacctgctgc ttctgctcgc ccatgacagc
      901 gacgccttca cgcgctggga agccgcacaa acgctctacc gccgtgccgt cgccgccaac
      961 cttgccgcac tttcagacgg tattgaactg ccgaaacacg aaaaactgct tgccgccgtc
     1021 gaaaaagtca tttcagacga cctcttagac aacgccttca aagccctgct tttgggcgtg
     1081 ccgtctgaag ccgagctgtg ggacggcgcg gaaaacatcg acccgctgcg ctaccatcag
     1141 gcgcgcgaag ccttgttgga cacgcttgcc gtccgcttcc tgccgaaatg gcacgaattg
     1201 aaccgtcagg cggcgaagca ggaaaaccaa agctacgagt acagccccga agctgccggc
     1261 tggcgcacgc tgcgcaacgt ctgccgcgcc ttcgtcctgc gcgccgaccc cgcgcacatc
     1321 gaaaccgttg ccgaaaaata cggcgaaatg gcgcaaaaca tgacccacga atggggcatc
     1381 ctgtccgccg tcaacggcaa cgaaagcgat acgcgcaacc gcctgctggc gcagtttgcc
     1441 gacaagtttt cagacgacgc gctggtgatg gacaaatatt tcgcccttat cggctcaagc
     1501 cgccgcagcg acaccctgca gcaggttcaa accgccttgc agcatccgaa attcagcctc
     1561 gaaaacccca acaaagcccg ttcgctcatc ggcagcttca gccgcaacgt cccgcatttc
     1621 cacgcagaag acggcagcgg ctaccgcttc atcgccgaca aagtcatcga aatcgaccgc
     1681 ttcaacccgc aggtcgccgc ccgtttggtg caggcgttca acctctgcaa caagctcgag
     1741 ccgcaccgca aaaacttggt gaaacaagca ttgcagcgca ttcgggcgca ggaaggattg
     1801 tcgaaagacg tgggcgaaat cgtcggcaaa attttggatt gaggccgtca aacagaaaaa
     1861 caatgccgtc tgatatagtt acacacacgt tttatcatca cttccccatc gttttattac
     1921 gcaatggcaa aacggccgca aagtcatat
//
LOCUS       DAWHXZ010000255         1924 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.255, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000255 DAWHXZ010000000
VERSION     DAWHXZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.255"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            54..1223
                     /gene="metZ"
                     /locus_tag="WIE35_001939"
     CDS             54..1223
                     /gene="metZ"
                     /locus_tag="WIE35_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ4154811.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            1318..1524
                     /locus_tag="WIE35_001940"
     CDS             1318..1524
                     /locus_tag="WIE35_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ4154812.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(1623..1735)
                     /locus_tag="WIE35_001941"
                     /pseudo
     CDS             complement(1623..1735)
                     /locus_tag="WIE35_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          470 a          494 c          497 g          463 t
ORIGIN      
        1 taaacaacgg gcgcacgctc cgttgccgaa cggataaaac cgaggaatca ccgatgagca
       61 aaaaactcca tccgcaaacg ctcgccatac gcggcggcaa agaacaaacc ggataccgcg
      121 aacacaatca ggcattgttc ctgaccagca gtttcatgtg ggacaacgcc caacacgctg
      181 ccgatttgtt ttcaaaaaaa atcaaaggct tcacttatac ccgtaccgcc aacccgacca
      241 cagccgcctt tgaaaaacgc atcgccgctt tggaaggtgc ggaacgcgcg gtcgccactt
      301 cgaccggcat gtccgcgatt caggcggcgt ttttcacctt tttgcaggcg ggcgaccatg
      361 tgatttccag ccgcagcctg ttcggcacga ccgtcggctt tatcaataac atcgttacca
      421 aattcggcat cgaagtgagt cacgtgtcgc cgactgatat aaacgaatgg aaagccgccg
      481 tcaaagccaa caccaaactg ctgtttttgg aaacgccgtc caatcccttg ggagaagtgg
      541 ccgacttgga agccttggcg gaattggcgc acggcatcgg cgcgctcttg gtggtggaca
      601 acagcctgct gtcgcctgtc ggctctcagc ctttgaaaca cggcgcggat atttccgttt
      661 cctccgcgac caaagccata gacggacacg ggcgcgtgat gggcggcgtg ttggcgggtt
      721 cggaagaact gatggcgcag gtcgccgttt actgcaactc ttgcgggctg gcaatgtcgc
      781 cgtttaacgc atggcagttg ttgagcggcg tggaaaccct gtctctgcgt atggaaaaac
      841 agttcgacaa cgccttgaaa atcgcgcaat ggctgcaagc gcagccgcag gttcaagccg
      901 tgtattacac cggattaccc gaccatcctc aggcggagct tatccgcaaa cagcaaaacg
      961 gcggcggcat cgtcatcggc tttgaagtcg ccgaccaagc agccgcgtgg aaagtcgtgg
     1021 acggcgtcga acttttctcc cgtaccgcca acctcggcga cgtgcgctcg accattaccc
     1081 atccgtggac aaccacgcac ggcaggatgc agcccgaaga aaaactcgcc gccaacatcc
     1141 gccccggatt ggtgcgcctg tcggtcggtt tggaatatgt tggcgatttg attgacgatt
     1201 tgaaacaggc actcgcgcgc tgattgcgtg ttcagacggc atcggcggaa aaacaccgca
     1261 aatgccgtct gaaaccctta tcttatatga catacacgtc aacaatagga aaaaaatatg
     1321 gatgaagcgg tttttgccga ttgggcgttg aaaatctgcc tgaccggctt gattattttt
     1381 ttgggtttta tcgtttggaa tttgggcaag gagtctaaag cgggcaaatt cggcatcgcc
     1441 attttgtttt tggttttggg cttgggcgtg tttggttttg tgttcaaaga gttattgatt
     1501 aagtttttgg tattgccgaa ataggtttgc ggtctgtcaa aatgccgtct gaaatcataa
     1561 aaagtaatct gcaccctaaa agtttgattt aaccgccaac tgattaaggt gcggattttt
     1621 atgacaaaat atagtggatt aacaaaaacc agtacggcgt tgcctcgcct tgccgtacta
     1681 tttgtactgt ctgcggcttc gtcgccttgt cttgatttaa atttaatcca ctataaaaat
     1741 catctttccg cagtcattcc cacggatgcg aaagcctgtc tttttgaatc ccggataatt
     1801 ttacatcgtc attcccgcgc aggtgggaat ccagatcatt gggtagcggc aatgttcaaa
     1861 agtcgtctga aaaatcagaa gttctagatt cccgttttta cgggaatgac ggaatttcag
     1921 acgg
//
LOCUS       DAWHXZ010000256         1921 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.256, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000256 DAWHXZ010000000
VERSION     DAWHXZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.256"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..700
                     /locus_tag="WIE35_001942"
     CDS             <1..700
                     /locus_tag="WIE35_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA ligase (NAD(+)) LigA"
                     /protein_id="HEZ4154813.1"
                     /translation="VGERTAKTLAQAFGTLERVRRAPEPVLACLPDIGTVVARSIAHF
                     FAQAEQQAMIDELLAAGVAPQAQAVSLPAAQYAEPQRWIARLPGFKISENKAQALWEL
                     AGKSIEGLQTDKSLPADWQAWRSEPQNAALLENLKTFFAQMPSEDEAAQGSDGINKAV
                     AGKTFVLTGTLPTLKRDQAQSLIEAAGGKVSGSVSKKTDYVVAGEAAGSKLEKANALG
                     VSVLSEAELLTLLG"
     gene            757..>1921
                     /locus_tag="WIE35_001943"
     CDS             757..>1921
                     /locus_tag="WIE35_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ4154814.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALEGAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQ"
BASE COUNT          466 a          615 c          489 g          351 t
ORIGIN      
        1 cgtcggcgaa cgcaccgcca aaacgctggc acaggcattc ggcacattgg aacgcgtccg
       61 ccgcgccccc gaacccgtcc tcgcctgcct gcccgacatc ggtacggtag tggcgcgttc
      121 cattgcccac ttcttcgccc aagccgaaca gcaggcgatg atagacgagc tgctcgccgc
      181 aggcgttgcc ccgcaagccc aagccgtcag cctgcctgcc gcacaatatg ccgaaccgca
      241 acgctggatt gcccgcctgc ccggtttcaa aattagcgaa aacaaagccc aagccttatg
      301 ggaactcgcc ggcaaaagta tagagggtct gcaaaccgac aaatccctcc ccgccgactg
      361 gcaagcatgg cgcagcgaac cacaaaacgc cgccctactc gaaaacctga aaaccttctt
      421 cgcgcaaatg ccgtctgaag acgaggcggc gcaaggttca gacggcatca ataaagccgt
      481 agcgggcaaa accttcgtgt taactggcac actgcccacc ctcaaacgcg accaagccca
      541 atccctaatc gaagccgcag gcggcaaagt ttccggcagc gtgtccaaaa aaaccgatta
      601 cgtcgtcgcc ggagaagccg cgggcagcaa gctggaaaaa gccaatgcct tgggcgtttc
      661 cgtcctcagc gaagccgaac tgcttaccct gctcggctga acccatgccg tctgaaccgc
      721 cttcagacgg cattgcccac ttttccgaac cacaccatgc acaccatttc ctttccaaac
      781 cgcacccgac tcaccgccct gccgcccctg tcgctttaca tccacatccc gtggtgcatc
      841 aaaaaatgcc cgtattgcga cttcaattcc cacagcctga aaaacggatt gcccgaagcc
      901 gcctatatcg acgcgctgct gaccgatttg cagcttgaat tgcccaatat ttggggcagg
      961 ccggtggaaa cgatcttttt cggcggcggt acgcccagcc tgtttcaggc ggaatcgatt
     1021 gaccgtttgt taagcggcgt gcgttcgctg ttgcgcttgc agccggaagc ggaaattacc
     1081 ttggaagcca atccggggac atttgaaatc gagaagtttc aaggatttaa agacgcgggt
     1141 attacgcggc tttctatcgg cgtacagagt ttcaacgacg atatgctctc aaggctgggg
     1201 cgcgtccaca acggtaagga agccttaacc gctatcgata ccgccttgaa attatttgat
     1261 aaagtcaata tcgacttgat gtatgccctg ccgaaccaaa ccgttcagac ggcattggac
     1321 gacgtacaaa ccgctatcgc tacgggcgcg acccacatca gcgcgtatca tctgacgatg
     1381 gaaccgaaca cgccgttcgg tcatacgccg ccgaaaggtt tgccgcaaga cgaagccgcc
     1441 ctcgacatcg aagacgcggt acacggcgcg ctggaaggcg cgggttttgt ccactacgaa
     1501 acatcggctt ttgcgaaacc agccatgcag tgccgccaca atttgaacta ctggcagttc
     1561 ggcgattatt taggcatagg cgcgggcgcg cacggcaaaa tttcctatcc cgaccgcatc
     1621 gagcgcaccg tccgccgccg ccaccccaac gactacctcg ccttaatgca aagccaaccg
     1681 agtgaagccg tcgaacgcaa aaccgtcgct gccgaagatt tgccgttcga attcatgatg
     1741 aacgccctgc gcctgaccga cggcgtaccc gccgcgatgt tgcaggagcg cacgggcgta
     1801 ccgagtgcca aaatcatggc gcaaatcgaa acggcaaggc aaaaaggcct gctggaaacc
     1861 gaccctgccg tattccgccc gaccgaaaaa ggacgcttgt ttttaaacga tttgctgcag
     1921 t
//
LOCUS       DAWHXZ010000257         1897 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.257, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000257 DAWHXZ010000000
VERSION     DAWHXZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.257"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1600
                     /locus_tag="WIE35_001944"
     CDS             <1..1600
                     /locus_tag="WIE35_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ4154815.1"
                     /translation="GKQAVGEIKRHSDGTPAENLIIKGNNLIALHSLAKQFKGKVKLI
                     YIDPPYYFVKKKQQDSFGYNTNFKLSTWLTFMKNRLSIAKELLTDDGIIVISIDDDGN
                     AYLKILLDEIFGFENFIGNLPTIMNLKGNNDEYAFAGTHEYTLVFAKNKDKSTFYEFP
                     IDEDNFLEKWEEDEIGFYKKGAPMRATGTEDKREDRPEMFYPFLVKNNTVSTITDEEF
                     SQIYNKDLEVFNDDFIQKLKEKYENLGYNFILPIADKQWGRWRWGYSIKNKARLQTDI
                     IVSQSKNGISLYKKQRPELDDLPTKKPKTIFYKPEYSSGNGTEQMKNLFGEKAFKNPK
                     PEELIQDFITITTNESDIVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERLK
                     KVIDGEQGGISKAVNWQGGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERY
                     FLKYNVSVNEFSQIIQEPEFQSLALDEQKQMVLEMLDLNQMYVSLSEMDDEQFAGCLN
                     DDDKALSREFYQSAKNQAEKKDGE"
     gene            1590..>1897
                     /locus_tag="WIE35_001945"
     CDS             1590..>1897
                     /locus_tag="WIE35_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ4154816.1"
                     /translation="MANNKTLFDWVEDRKSTLEEIEQTDFFALPEFVERNLKYPFFEW
                     QKSALENFVIFDRASKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     "
BASE COUNT          684 a          299 c          360 g          554 t
ORIGIN      
        1 cggcaaacaa gcggttggcg aaatcaaacg tcattcagac ggcacacccg ccgaaaatct
       61 cattatcaaa ggcaataatc tgatagccct acattcgctt gccaaacagt ttaaaggcaa
      121 agtgaagttg atttatattg atccgcctta ttattttgtt aaaaagaaac aacaagatag
      181 ttttggttat aacactaact ttaaattatc tacttggctt acatttatga aaaatcgttt
      241 atcaattgcc aaagaattat taactgatga tgggattatt gttatttcca ttgatgatga
      301 cggaaatgct tatttaaaga ttttgttaga tgaaattttt ggatttgaaa attttatcgg
      361 aaatttaccc actattatga atttaaaagg taataatgat gaatatgctt ttgcaggcac
      421 tcacgaatac acactagtct ttgctaaaaa taaagataaa tctacttttt atgaatttcc
      481 aatagatgaa gataattttt tagagaaatg ggaagaagat gaaattgggt tttataaaaa
      541 aggagctcca atgcgagcaa caggaaccga agataaaaga gaagatagac ctgaaatgtt
      601 ctatcctttt ttggtaaaaa ataacacagt tagtacaatt acagatgaag aattttctca
      661 aatctataat aaagatttag aagtattcaa tgatgatttt attcaaaaac taaaagagaa
      721 atacgaaaat cttggataca attttatttt acctattgct gataaacaat gggggcgttg
      781 gcgttggggg tattcaataa agaataaggc aaggctgcag acggatatta ttgttagcca
      841 atctaaaaat ggaatttcct tgtataaaaa acaaagacct gaattagatg atttacctac
      901 aaaaaaacct aaaactattt tttataaacc tgaatacagt agtggtaatg gaactgaaca
      961 gatgaaaaat ctttttggtg aaaaggcatt taaaaatcct aaaccagaag aacttatcca
     1021 agattttatt accatcacaa ccaacgaatc cgacatcgtc ttagactacc atcttggtag
     1081 tggcacaacc gccgccgttg cccacaaaat gaaccgacaa tatatcggta ttgaacaaat
     1141 ggattatatt gaaacgcttg ccgttgaacg cttgaaaaaa gtgattgatg gcgagcaagg
     1201 cggtatttcc aaagccgtga attggcaagg tggcggcgag tttgtctatg ccgaacttgc
     1261 tccatttaac gaaaccgcaa aacaacaaat tttggcttgc gaagattcag acggcatcaa
     1321 aacgctgttt gaaggtttat gcgaacgcta tttcttgaaa tacaacgtca gcgtaaatga
     1381 atttagtcaa atcattcaag agcctgaatt tcaatccttg gcattagacg aacaaaaaca
     1441 aatggtgctt gaaatgttgg atttaaatca aatgtatgtt tcattatccg aaatggatga
     1501 tgagcaattt gcaggttgct taaatgatga cgataaagcg ttaagccgtg aattctatca
     1561 atcagcaaaa aatcaagcgg agaaaaaaga tggcgaataa taaaacgtta tttgattggg
     1621 tggaagaccg caaatcaacg ctggaagaaa tagaacagac agattttttc gcgctgcctg
     1681 aatttgtaga acgcaattta aaatatccgt tttttgaatg gcaaaaatcg gctttagaaa
     1741 actttgtgat ttttgaccgc gcttcaaaat taaaggattt ccctgacatc aaaaaccgcc
     1801 ctacccattt gctgttcaat atggcaacag gtgctggcaa aacgatgatg atggcggcgt
     1861 tgattttgta ttattttgaa aaaggttatc ggcattt
//
LOCUS       DAWHXZ010000258         1892 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.258, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000258 DAWHXZ010000000
VERSION     DAWHXZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.258"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            235..1194
                     /locus_tag="WIE35_001946"
     CDS             235..1194
                     /locus_tag="WIE35_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154817.1"
                     /translation="MYQNYDIVILSLGNGLLTRFFRNSSKLNLTLPIVITLFPGVIFG
                     DSESIITRLGANILLFNNYYDYQIGNKFKERYNLSCTNILYGYPILRKKHHCSDGNKV
                     YFIDQVKIPDGKKERLLILKKLIALANRYPHKDLVLLLRVHQNDITVHEDKYSYAELA
                     KNMKLPDNFKIERKRTQDALQEMGYCLSFSSTMLFEAESCGIPIGVISDLGVDKKYAN
                     THFKDSGVLVNLDTVDLDSPNTINPTWAELYTNPDSLSSKEFNQLLEVTSTQPPSFDN
                     YFIAVNDVQPVSMVLLRKFKKLIYHPKQFFVDSKLYKLIKFTK"
     gene            1242..>1892
                     /locus_tag="WIE35_001947"
     CDS             1242..>1892
                     /locus_tag="WIE35_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEZ4154818.1"
                     /translation="MQKIRKAVFPVAGMGTRFLPATKASAKEMLPIVDKPLIQYAAEE
                     AVAAGCTELIFITGRNKRNIEDHFDKAYELETELEMREKHKLLGYVQKILPDHVSCIF
                     IRQAEALGLGHAVLCARPAVGNEPFAVILADDLIDAPQGALKQMVDIYNQSGNSILGV
                     ETVDPSQTGSYGIVEVEQLKSYQRVTNIVEKPKPEDAPSNLAVVGRYILTPRIFDLL"
BASE COUNT          635 a          309 c          335 g          613 t
ORIGIN      
        1 ttaatatcta atatttaaaa tacaatgaat aaaaaacgag tcctattaat tgctagttat
       61 gattcttttt tgaatactgg atatgctgtt gcaaaagaaa ttaactattc aacaatcgat
      121 atttatatcc accaaacaaa aaaaaatatt ctttcggaat cacaattaag tttaaatggg
      181 ctttctttaa aagaaatcaa tttttttcat attgatgatt atttcgacaa attaatgtac
      241 cagaattatg atattgttat tctatcatta ggtaatggtt tactgacgcg tttttttcgg
      301 aatagtagca aacttaattt aacattacct atagtaatta ctctatttcc aggggtaatt
      361 tttggtgatt cagaaagtat tataactagg ttaggtgcta atattttatt gttcaataat
      421 tattatgatt accaaatagg caataagttc aaagaacgtt acaacttatc gtgtacaaat
      481 attttatatg gttatcctat cctacgaaaa aaacaccatt gctctgatgg caataaagtt
      541 tatttcattg atcaagtaaa aattccagat ggaaaaaaag aaagattatt aattctaaaa
      601 aaattaatag cactggctaa tcgctatcca cataaagatt tagtactatt attacgtgtc
      661 catcaaaatg atatcacagt acatgaagat aaatactctt atgctgaatt agcaaaaaat
      721 atgaaattgc ctgataattt taaaatagag agaaaacgga cacaagatgc cttacaagaa
      781 atgggatatt gtttatcatt ctcttcaaca atgttatttg aagctgaaag ctgcggaatt
      841 cctattggtg tcattagtga tttaggggtt gacaagaaat atgccaatac acatttcaaa
      901 gatagtggtg ttttggttaa tttagatacg gtagacttgg actctccaaa taccattaat
      961 ccaacttggg ctgaactata tactaatcca gatagcctct cttcaaaaga gtttaaccaa
     1021 ttattagagg ttacttctac tcaaccacct agttttgata attattttat tgcagttaat
     1081 gacgttcagc ctgtatctat ggttctgtta cgtaaattta aaaagcttat atatcatcct
     1141 aagcaattct ttgttgattc aaaactatat aaattaatta aatttactaa ataattttta
     1201 ctatgggttg ccttatttat ttcaaaggag tgacataaaa aatgcaaaaa attcgtaaag
     1261 ctgtattccc tgtagctggc atgggaaccc gattcttacc tgccacgaaa gccagcgcta
     1321 aagaaatgct gccaatcgtg gacaaaccgt taattcaata tgctgcggaa gaagccgttg
     1381 ccgcgggttg tactgaattg atttttatca ccggccgtaa taagcgtaat attgaagacc
     1441 acttcgataa agcctatgag ttggaaactg aactggaaat gcgtgaaaag cataaattgc
     1501 ttggctatgt gcaaaaaatt ctgcctgatc atgtcagctg tattttcatt cgtcaggcag
     1561 aagctttggg cttgggtcat gcggtattat gcgcccgtcc tgctgtcggt aatgagcctt
     1621 ttgccgtgat tttggccgat gacttgattg atgcaccaca aggcgcactc aagcagatgg
     1681 tagatattta caatcaaagc ggcaacagca ttttaggggt agaaacagtc gatccttcgc
     1741 aaaccggttc atacggcatt gtcgaagtgg aacagctgaa aagctatcag cgtgtaacca
     1801 atattgtgga aaaaccaaag cctgaagatg cgccgtcaaa tttagcggta gtcggccgtt
     1861 atattttgac accgcgtatt tttgatttgc tg
//
LOCUS       DAWHXZ010000259         1801 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.259, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000259 DAWHXZ010000000
VERSION     DAWHXZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.259"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            4..303
                     /locus_tag="WIE35_001948"
     CDS             4..303
                     /locus_tag="WIE35_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ4154819.1"
                     /translation="MEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREGGR
                     VADSEKIVIMATLNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            309..488
                     /gene="ssrS"
                     /locus_tag="WIE35_001949"
     ncRNA           309..488
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WIE35_001949"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            666..1097
                     /gene="rplM"
                     /locus_tag="WIE35_001950"
     CDS             666..1097
                     /gene="rplM"
                     /locus_tag="WIE35_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ4154820.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            1107..1499
                     /gene="rpsI"
                     /locus_tag="WIE35_001951"
     CDS             1107..1499
                     /gene="rpsI"
                     /locus_tag="WIE35_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ4154821.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            1650..>1801
                     /locus_tag="WIE35_001952"
     CDS             1650..>1801
                     /locus_tag="WIE35_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ4154822.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRG"
BASE COUNT          492 a          506 c          419 g          384 t
ORIGIN      
        1 aacatcgaac aagtccacat cgaagtcatg cacgcccggc tgaccgtcaa cacgccggca
       61 gaagaaaaag acacactgtt gcaggcagtc ggaatgctca acggcaaagc cgaagccatc
      121 cgcgaaggcg gacgcgtcgc ggacagcgaa aaaatcgtca ttatggccac gctcaacgtc
      181 gtccacgacc tgttgaaaac ctccctgaac ggcggcgatt tggcaatcgg cgattttgcg
      241 cgtaaaatag ccgatatgga caacgcctgc caaaaagcac tatcccgctt ggcgcaggaa
      301 taaccccttt ttccctgcgg tgtccgcgaa ggcatatatt cctttgaacc aataagttcg
      361 cttaaggttg ccgggagcag tagtgggcgc gagcgtcctt ttgcggacgc acccgaaact
      421 acccgaggca gccgccttgt cagcaaggtt caagcggatt cggccgaaac ggctctcgcg
      481 gggattccca ttcaaaaccg catcgcaacg atgcggtttt tcacattggc cgccgccccg
      541 cccgaaaaag cagtatgcag tctgccgaaa tccggaaaat ccttccgtaa cgcggtaatg
      601 cgattgacaa aatcgaatcc tgtctttaaa atttacaact ttctatatct atctggattt
      661 ccactatgaa aaccttttca gcgaaacccc acgaggtgaa gcgcgaatgg ttcgtcatcg
      721 atgcccaaga caaagtcttg ggtcgcgttg cggccgaagt cgccagccgt ctgcgtggca
      781 aacacaaacc tgaatacacc ccccacgtcg ataccggcga ttacatcatt gttatcaatg
      841 cggacaaact gcgtgtaacc ggtgccaaat tcgaagataa aaaatacttc cgccattccg
      901 gtttcccagg cggtatctac gaacgcactt tccgcgaaat gcaagagcaa ttcccgggcc
      961 gcgctttgga acaagctgta aaaggtatgc tgcccaaagg tcctttgggt tacgccatga
     1021 ttaaaaaact gaaagtgtat gcgggtgcgg aacacgccca tgctgcgcaa caacccaaag
     1081 ttttggaact gaaataagga cgcgacatga acggtaaata ctactacggc acaggccgcc
     1141 gcaaaagttc agtggctcgt gtattcctga ttaaaggtac aggtcaaatc atcgtaaacg
     1201 gccgtcccgt tgacgaattc ttcgcacgcg aaaccagccg aatggttgtt cgccaaccct
     1261 tggttctgac tgaaaacgcc gaatctttcg acatcaaagt caatgttgtt ggcggcggcg
     1321 aaaccggcca gtccggcgca atccgccacg gcattacccg tgccctgatc gacttcgatg
     1381 ccgcgttgaa acccgccttg tctcaagctg gttttgttac ccgcgatgcc cgcgaagttg
     1441 aacgtaaaaa accgggtctg cgcaaagcac gccgtgcaaa acaattctcc aaacgttaat
     1501 gttggaaatt caaaaaaccc tgcttatcgc agggtttttt atttgtagta gacggtttcc
     1561 cattggcaat ctaaagatta cagattgaga cctttgcaaa attccccaaa atcccctaaa
     1621 ttcccaccaa gccatttagg ggattttcca tgagcacctt ctttcagcaa accgcacaag
     1681 ccatgattgc caaacacatc gaccgcttcc cactattgaa gctagatcaa gtgattgatt
     1741 ggcaaccgat cgaacaatac ctgaaccgtc aaagaacccg ttacctccga gaccaccgcg
     1801 g
//
LOCUS       DAWHXZ010000260         1791 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.260, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000260 DAWHXZ010000000
VERSION     DAWHXZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.260"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(148..804)
                     /locus_tag="WIE35_001953"
     CDS             complement(148..804)
                     /locus_tag="WIE35_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ4154823.1"
                     /translation="MKNKSKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIRDYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQIGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFNLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALAAAAILLLS
                     "
     gene            869..1759
                     /locus_tag="WIE35_001954"
     CDS             869..1759
                     /locus_tag="WIE35_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ4154824.1"
                     /translation="MFPVFHLSGESRRRMLQAALRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLTKRQG
                     INAWVVGCGLGTDKAAVGTLAGILTEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIRAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
BASE COUNT          406 a          425 c          583 g          377 t
ORIGIN      
        1 ccttgtcctg atttttgtta atccactata aaacggtttg ggacggaaaa acggcctgaa
       61 gccgtttcgg gcattcagac cgtttgcgcg gagaggggat gccgtccgaa gggcgaaaag
      121 ggcttcagac ggcattgatg tcgggtttca ggacaggagc aggatggcgg ctgcggcaag
      181 cgcggcaacc gataatgcgg cggcaagcgc ggttttaccg gcaaagcgga ttgaggtttt
      241 gccttcgatg tatttgaagc cggttatcat cgggaggacg aggtttttct ttttgaacac
      301 gcggtatgcg gcgacggcgg cgatatgaat ggcggaaaaa acggcgagca ggttgaaaaa
      361 gttgaggtgg attttccgca taaggctgcc tgtatgttcg gaaaccaaat ggttgaggta
      421 gccgttggtg ctgaaggtgt tttcatcggc ggcaaaaagc cccgtgccga tttgaaatga
      481 gacggcggcc aaaagcgcga cgaccatcag tgcgcccaag gggttgtgtc cgggctggat
      541 atgttcggga ataccgtttt tcagataatc cctgataccc gaccatccgc ggacgaaacg
      601 ggagaaacgg gcggtatcgc tgccccaaat gccccagcag aggcggaata cgagcaggaa
      661 aaggataaac agcccgacgc gcgtgtgcca ttgaagcata tcgccgccgg cttttgcgct
      721 ataccacata aagggcaagg acgcggcaag cagccagtgg aaaaggcggg tggggaggtc
      781 ccagactttg cttttgtttt tcataatcgg tttccggcgg tagaatcggt ttgttttcga
      841 gccttaattt taaccgattg gaggggaaat gtttcccgtt ttccatcttt caggcgaaag
      901 ccgccgccgg atgcttcagg cggcattgcg ttttccctat gttttcaaag cccgtgcgga
      961 agattcgcac aaagggactt tcggcacgct cgccgtagtc ggcggatcgg cagggatgag
     1021 cggtgcgccc gtattggcgg catcggcggc gatgtatctc ggctgcggca aggtgtgggc
     1081 gggtttcaat caggatgcgc ttccgtttgc cgtcattgcc ggtttccccg aaattatgtt
     1141 ggacacggca gacggtttga ccaagcgtca aggcataaac gcctgggttg tcggttgcgg
     1201 cttgggtacg gataaggcgg cggtcggaac gcttgccgga attttgacgg aacacacgga
     1261 caagcccgtc cttttggatg cggacgcgct gaacatatta tcaaccgatg ccgaaacccg
     1321 aaatctggca cgcgggtgta aaaacctgat tttaacgcca caccccgccg aagccgcgcg
     1381 cctgcttgga acgacggttg cacaggttca ggcggatcgg acggcggcag tgaggaagat
     1441 aagggcaatt ttcggcgcaa ccgtggtttt aaaggggcac aaaacattgg ttgcctcacc
     1501 cgatacggaa atctatgtca acgaaagcgg caacgcggga ttggcaacgg cgggcagtgg
     1561 cgacgtattg ggcggcatca tcggcagtct gctcgcacag ggcgtgccgg tttttgaagc
     1621 cgcctgcgcg ggcgcgtggc tgcacggcgc ggcggcggat gtcataaaag aatcggcagg
     1681 cattgcggca gggctgttgg ctggcgaaat cgccccggcg gcaaggtggc tgcgcaaccg
     1741 gataactgaa agtatgtaag aagatatagt ggattaacaa aaaccagtac a
//
LOCUS       DAWHXZ010000261         1773 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.261, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000261 DAWHXZ010000000
VERSION     DAWHXZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.261"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(150..665)
                     /locus_tag="WIE35_001955"
     CDS             complement(150..665)
                     /locus_tag="WIE35_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154825.1"
                     /translation="MSAFRKILLIISCLLIASCSFVETIFYMAISPEPVVVDFPLGKK
                     TKRSIELKQKIGKPYAISLGTNFIHYDPKQGERWIDDKLNYPYNISVKIFKVEEDGKK
                     LIIDELLTERSRKLGGGVFGAGGKYSMYIYDFYLPEGEYLFEISDNSEYIPLYDEINN
                     SIRIVVNVRIQ"
     gene            complement(959..1483)
                     /locus_tag="WIE35_001956"
     CDS             complement(959..1483)
                     /locus_tag="WIE35_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpA"
                     /protein_id="HEZ4154826.1"
                     /translation="MENNKAKSDDNSVSIEFIQDFITASTDVINLSYENFRKNFYTQM
                     STDSTNYAAKHESLGKSVQRELQKTQSQLRQVVRKMQSKYNINNKARAAEISLLRQMQ
                     SQFSRKDVNKNLGNGNTLAQQGSYTKKDGTTAQAGDLLLAADNLHSRLTDKMLSISHV
                     RENTISPFVLGCLK"
BASE COUNT          597 a          313 c          225 g          638 t
ORIGIN      
        1 atttttttat ctctagccga atacagttca acccaaacgt acccgtcttg gatacacccc
       61 ctcttgtttc agtggcgtac cctgcttcgc aaattttaca gttatcgcat aataatcagc
      121 cataagtaac cccacatttc tagaaaaatt tactgaattc gtacattaac tactattctt
      181 atagaattat ttatttcatc gtaaagtgga atatattcac tattatcaga aatctcaaat
      241 aaatattccc cttccggcaa ataaaaatca taaatataca tactgtattt tcccccagct
      301 ccaaatactc cgcctcctaa ttttctactt ctctctgtaa gcaactcatc tataataagt
      361 tttttaccat cttcttccac tttaaatatt ttaaccgata tattatatgg atagtttaac
      421 ttatcatcaa tccacctctc cccctgtttt ggatcataat gtataaaatt agttcctaac
      481 gatattgcat aaggtttacc aattttctgt ttgagttcaa tagatctttt tgttttttta
      541 ccaagaggaa agtctaccac aacaggttct gggctaatag ccatataaaa aatagtttca
      601 acaaaactgc agctagcaat caataggcaa gatattatta ataatatttt tctaaatgca
      661 gacataataa aaatttccaa aaaatccata tcagaatata ctaattattt gtataattaa
      721 taaggaagag aaaaattcaa aacggctatg agatttccgc ctgaaaataa tgaaataaaa
      781 gtccatagta atgaataata gctttatcag acccccttta atgcaaacat agagaatact
      841 tcccttgaag cactatcaat ttctacttaa ctaaaactct agctctattt ctaaaatttt
      901 gtcaaaaata gttaaatact tttatttatt ttaaataatg atatgcattt atggttattt
      961 attttagaca gcctaaaaca aacggggaaa tcgtgttttc ccgaacatgg ctaatggata
     1021 gcattttgtc cgtgaggcgg ctgtgcaggt tgtcagcagc caacagcaaa tcgcctgctt
     1081 gcgcggttgt gccgtctttt ttggtgtagc tgccttgttg agccaaagtg ttgccgttac
     1141 caagattttt gtttacatct tttcgagaaa attggctttg catttgcctt aacaaagata
     1201 tttctgctgc tcgtgcttta ttatttatat tatatttact ctgcattttt cttacaactt
     1261 gtctcaactg actttgtgtt ttttgtaatt cacgttgtac cgattttcct aaactttcat
     1321 gtttggctgc ataattggta gaatcagttg acatttgtgt ataaaaattt ttacgaaaat
     1381 tttcgtaact cagattaatt acatccgtac ttgcagttat aaaatcttgt ataaattcaa
     1441 tagaaactga attatcatca gatttggctt tgttattttc catattaagc ctctcttaat
     1501 ttgaaaaaat taacataaac atttaaaaat aaaacaaaat tatataataa aaaaaataat
     1561 gagaatataa aggataaaaa gagagttaag ttatttaatc ggacttgttc tctataaatc
     1621 ataaggcacc aaataagcca taataaattt agtaaataaa aactgcttat acccaacttt
     1681 cttttcttgg aacctattat tatttcaact ctggatgaaa aaatagaatt taaaaatttt
     1741 tttctttgat atatcgttaa tatctcccta gtt
//
LOCUS       DAWHXZ010000262         1763 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.262, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000262 DAWHXZ010000000
VERSION     DAWHXZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.262"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..246
                     /locus_tag="WIE35_001957"
     CDS             <1..246
                     /locus_tag="WIE35_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ4154827.1"
                     /translation="PRVIIEGSKMDALYVSAPFYFDDDFQVCYGGHYNTVFPLLVPLY
                     KQEAELVEKKGWNAFEQFLLDNEVDNLSDMNRKPFVW"
     gene            382..1050
                     /locus_tag="WIE35_001958"
     CDS             382..1050
                     /locus_tag="WIE35_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ4154828.1"
                     /translation="MVKTADGYKAIAHIQAGDRVFAKDETSGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNSCPPKRAPEYHAGTVSESNFLNVA
                     EKWLGENYKSYPNGRYVSQDGMRQVRYGHHETNSSTHHGHFESYDKPNGRVIENSVVT
                     IIQD"
     gene            1057..1326
                     /locus_tag="WIE35_001959"
     CDS             1057..1326
                     /locus_tag="WIE35_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154829.1"
                     /translation="MSVKELFKVILDKNKDFPIRTIHRTPMGVLPKPVALSIVQYEDD
                     QGFYLFYLDETGREQTDTYHDTLDSAFKQAEFEFGIRKEEWIQSS"
     gene            1486..>1763
                     /locus_tag="WIE35_001960"
     CDS             1486..>1763
                     /locus_tag="WIE35_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ4154830.1"
                     /translation="MIIVEHLEHYLGEIENGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRKPI"
BASE COUNT          582 a          321 c          355 g          505 t
ORIGIN      
        1 cctagggtaa ttatcgaagg tagcaaaatg gacgctctgt atgtttccgc cccattttat
       61 ttcgatgatg attttcaggt ctgttatggc ggacactaca atactgtttt tcctttgctt
      121 gtcccgttgt ataaacagga agcagaattg gtggaaaaaa agggttggaa tgcttttgag
      181 cagttcttgc tggataatga agttgacaac ctttcggata tgaataggaa accgtttgtt
      241 tggtaaagga ttttcttaaa tttgattaaa ggtcgtctga aattaagctt gtttttcaga
      301 cgacctttcc ttcatttgaa acagaatatt gagaactgag ttcttcaaaa atcctacacc
      361 tgctccttcc acggcagcac cttggtcaaa acggcagacg gctacaaagc cattgcccat
      421 attcaagccg gcgaccgcgt cttcgccaaa gatgaaacca gtggaaaaac tggatacaaa
      481 cctgttaccg cccgatacgg caatccgtac caggaaaccg tttacattga aatttcagac
      541 ggcatcggta acagccaaac cctaatttcc aaccgcatcc atccgtttta cagtcaagga
      601 aaatggatac aggcaggtcg tctgaaaaaa ggcgacaccc tgctttccga aagcggtgca
      661 aaacagacag ttcaaaacat taccctcaaa cagcagccgc tcaaagccta caatctgacc
      721 gttgccgatt ggcatactta cttcgttaag ggtaatcagg cggaaacgga aggggtttgg
      781 gttcataatt cttgtcctcc caaaagagct cccgaatacc atgcaggaac tgtatccgaa
      841 tctaattttt taaatgtagc tgagaaatgg ctaggagaaa actataagtc atatccaaat
      901 ggtcgatatg tatcacaaga tggaatgcga caggtacgat atggtcatca tgaaacaaat
      961 tcatctacac atcatggtca ttttgaatca tatgacaaac ccaatggacg tgtaatagaa
     1021 aatagtgtag taacaattat tcaggattaa aaaattatga gtgtaaaaga attattcaaa
     1081 gttatcttag ataaaaataa agattttcct attagaacga ttcatcgcac tccaatgggg
     1141 gtacttccca agcctgttgc tttaagtatt gttcaatatg aagatgacca aggattttat
     1201 ttattttatt tggatgagac cggtcgggaa caaacggata cttatcatga cacattagat
     1261 tcggcattta aacaggctga atttgaattt ggaattagaa aagaagaatg gatacaaagt
     1321 tcttaggtat caaacgtaag atgggttgaa aaaatcaaca atcaaaaggt cgtctgaaac
     1381 catgtttggt ttttcagacg acttttccgt taataaaaaa tagcgatatt ggaacccaaa
     1441 ccagcctacg gcttgttaga atagggatga aaagtttaag aaaaaatgat tatagttgaa
     1501 catttagagc attatttggg cgaaatcgaa aacggtataa aatgcttgga tcgacgttat
     1561 catctcagcg tttccgtatt cccatcacaa ccatataaag gggtaactac tttttctact
     1621 ttaggtttga atcgatatga cttgaactat aagagtaggt ttgaattaat ttttacctgt
     1681 tctgaagaat ggaataaaga aaatattgca gcttttctat caggagtagc tgaatatttg
     1741 attgacaata gaaaacctat ttt
//
LOCUS       DAWHXZ010000263         1756 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.263, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000263 DAWHXZ010000000
VERSION     DAWHXZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.263"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(42..365)
                     /locus_tag="WIE35_001961"
     CDS             complement(42..365)
                     /locus_tag="WIE35_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ4154831.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IIPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(353..592)
                     /locus_tag="WIE35_001962"
     CDS             complement(353..592)
                     /locus_tag="WIE35_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ4154832.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(715..1512)
                     /gene="truA"
                     /locus_tag="WIE35_001963"
     CDS             complement(715..1512)
                     /gene="truA"
                     /locus_tag="WIE35_001963"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ4154833.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGPMRRAAALLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          387 a          415 c          513 g          441 t
ORIGIN      
        1 ttaatccact ataatgaaca caatccattc agactattca atcaggcaaa catctcctgc
       61 aatactgcaa acagtttttc agccgtactg ttgtctaaat ttccaagatg tttgaccaat
      121 ccggctttat ccacagccct aatctgttcg ggcaaaagca aaccgtcttt atcctgaaag
      181 cggacattga cgcggaacgg ggcaggacgg cttccgctcg tcatgggaat gatcagcaca
      241 gtcttgagat agttgtgtat ttcaggagga gagactacga cacaaggacg tgtctttttg
      301 atttcgcttc ctacggtcgg gtctaaggag accagataga ttccgccgcg tactaccata
      361 tccattcttt atccgcttcg ttttcaattt cggaaaaaaa atgctcctgc tcggtttcga
      421 caagcattgc ggcagcttct gcccatcccc tgcgaacggt aggacagctt aaaatgatat
      481 tgcccttttc aactgtaaca gccaagctgt ctactgcccc tatttgaccc aataatgatt
      541 tgggcagaat cacgccttgc gagtttccca ttttgcgtat gttgagaatc atatacgtac
      601 cctatttacc agtttatgta ataacaatgt tattaccttg atgaggtagt gtcaatattg
      661 aaaaagattg cctgacagtt tgtccgattt caaaatctcc gcgacaagaa tgttttaaag
      721 ccattcgggg atttgggggc ggatgatgcc gtatgcctcg ggatagtcga cgccggtcag
      781 gtaaagtccg tcgggcatga aggtcggcgg ggctttgagg cggctgcgtt cttgaatcag
      841 tgcggcgaag ccttcgacgc tgagtctgcc gctgccgaca tagacgagcg cgcccatgat
      901 gttgcgtacc atgtggtgca aaaaggcgtt gccgtgcaaa tcgaggcgga cgagtcctgc
      961 gctttgggta aggtcggcgc ggtagatggt tttgacgggg gattttgcct ggcattcggc
     1021 ggcgcggaag ctggagaagt cttgttcgcc gatcaataag gcggcagccc ggcgcatcgg
     1081 cccgatgtcg agtttgaggt gtgtccagcc tgccctgttt ttgagcaggg gggaacggac
     1141 gggggcggat tcgagcaggt agcggtaatg ccgtccgtat gcgtcgaagc gggcgtgaaa
     1201 ttcgggggcg acctgtcggg cgtgcaaaac ggcaatgcct tcgggcaggt gggcatttac
     1261 gccgcgcacc catgcctgtt ggggacgggc ggcagttgtg tcgaagtgga cgacttgggc
     1321 ggtggcatgc acgccggtgt cggtcctgcc ggcaacggtg gtggaaaccg cttcccctgc
     1381 tatttgggcg agcgcggttt ccaatgccgc ctgaacggtc ggtacgccgt cagcctgttt
     1441 ctgccagccg taaaagcggc tgccgtcata ggatagggtt attgcccagc gttgtttttg
     1501 tgcggtatcc atcggatttg ggattcggat aaatgttcag actgcattgt atcgcagatt
     1561 ttgcagggaa acggcaaatg cccggggcgc gcggcgttgt ttggggagtt gggggggggt
     1621 ggatgcagtt gctacgaatc gctatcctgt gaattcaccc tgtcaggagt gcccgaatcg
     1681 tcattcccgc gcaggcggga atctaggacg taaaatctaa agaaaccatt ttatagtgga
     1741 ttaacaaaaa ccagta
//
LOCUS       DAWHXZ010000264         1755 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.264, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000264 DAWHXZ010000000
VERSION     DAWHXZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.264"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            210..881
                     /locus_tag="WIE35_001964"
     CDS             210..881
                     /locus_tag="WIE35_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ4154834.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
     gene            943..>1755
                     /locus_tag="WIE35_001965"
     CDS             943..>1755
                     /locus_tag="WIE35_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ4154835.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLELINRQLTEKGLKIEKASAAVVDATIIQTAGSKQRQAIE
                     VDEEGQINGQTTPSKDKDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECK
                     HLSPLLEGLPKGTTVYADKGYDSAENRQHLEEHQLQDGIMRKACRNRPLSEAQ"
BASE COUNT          503 a          465 c          388 g          399 t
ORIGIN      
        1 tcaaccgata ttcaaacaca gtcattttta gcgcattttc agcgtatcgt taatgcggaa
       61 aatttcgtga acaggttttt tgcacaggtc tccgcctcgt ttcgcgggat gggaaaccgt
      121 attagaaaac ggacgcacgc aacataaacc ccgaaagtga tgataagatg atgatttaac
      181 gtactgcttt aattatttaa ggaattatcg tgtttcccga caaatacaag ttgagtttgg
      241 aagaaaatat ttttctggca aagaaagtat tggttgccca aattcacaac ctcagccgtt
      301 ttgagaattg tcagacgacc ttgttgcaga ccgaacaaat tatccatggc aaaaatgtag
      361 cctccgcgtc actggaagac atccaaacca tcttgaacct gaaacgtgcc tatcaatatg
      421 tgatttcgca tatttcaaac ggcgaaccgg tcgatatttc actccttaaa aaaatcaaca
      481 acattgttgc caaggacgat tctttggcac ccggtgattt ccgtaccggt tcggtcggcg
      541 taacgctatt ggacggttcc cgtcatgccc caaatccggt gaaggaaatt gaagtggctc
      601 gcgtgttaca aaatatcgga ctgcaaagcg gttcgacgac ggaggcagcc gtccgtttta
      661 tgctttattg tatgcggcag caggtttttt gggacggcaa caaacgaacg gcaaccttat
      721 ttgccaacgg tctgatgatg gcggggggct gcggcatctt ggaaatctcc gaaatgcaga
      781 tgccgcaatt caatgaaaaa ctgtccgcat tctatcgctc cggcgacgat accgatattt
      841 ccaagtttgt gtatcaaaat tgtatatcgg gcatagactg agacctttgc aaaattcccc
      901 aaaatcccct aaattcccac caagacattt aggggatttc tcatgagcac cttcttccag
      961 caaaccgccc aagccatgat tgccaaacac atcgaccgtt tcccgctatt gaagttggat
     1021 caggtaattg attggcagcc gatcgagcag tacctgaacc gtcaaaaaaa ccgttacctc
     1081 cgagaccacc gcggccgtcc tgcctatccc ctgctgtcca tgttcaaagc cgtcctgctc
     1141 ggacaatggc acagcctctc cgatcccgaa ctcgaacaca gcctcattac ccgcatcgac
     1201 ttcaacctgt tttgccgttt tgacgaactg agcatccccg attacagcac cttatgccgc
     1261 taccgcaact ggctggcgca agacgacacc ctgtccgaac tgttggaact gattaaccgc
     1321 caactgaccg aaaaaggttt aaaaatagag aaagcatccg ccgctgtcgt tgacgccacc
     1381 attattcaga ccgccggcag caaacagcgc caggccatag aagtcgatga agaaggacaa
     1441 atcaacggcc aaaccacacc gagtaaagac aaagatgccc gctggataaa gaaaaacggc
     1501 ctctacaaac tcggttacaa acaacatacc cgtaccgatg cggaaggcta tatcgagaaa
     1561 ctgcacatta ctcccgccaa tgcccatgag tgcaaacacc tgtcgccttt gctggaagga
     1621 ctgcccaaag gtacgaccgt ctatgccgac aaaggctatg acagtgcgga aaaccggcaa
     1681 catctggaag aacatcagtt gcaggacggc attatgcgca aagcctgccg caaccgtccg
     1741 ctgtcggaag cgcaa
//
LOCUS       DAWHXZ010000265         1739 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.265, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000265 DAWHXZ010000000
VERSION     DAWHXZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.265"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            177..1151
                     /locus_tag="WIE35_001966"
     CDS             177..1151
                     /locus_tag="WIE35_001966"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ4154836.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALCGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPAKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(1310..1528)
                     /locus_tag="WIE35_001967"
     CDS             complement(1310..1528)
                     /locus_tag="WIE35_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ4154837.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT          478 a          483 c          372 g          406 t
ORIGIN      
        1 aatttatcca ctataagaat aaatatcccc tcatccgcct gccgcgttcc gacttgcccc
       61 gcgccgtttt tttccactac aatcccacgc gatatctggc ggaacgcacc aaaggaacgc
      121 cccgcagcaa tctgccgcct ttcagacggc acaaacattt ccgaaacgag caaactatgg
      181 acacactgac ccgattcctc cccgaacacc tgcaacaaaa ccagctgccc gaagcactct
      241 gcggcgtttt actgtccgtt gtttccgcct gcaccgaaat caacgccaaa gtccgcctcg
      301 gcgcactggc cggcgtattg ggtatggcgg gcacgggcaa tattcagggc gaagaccaaa
      361 aaaaactgga tgttatcgcc aacaacatca tgattgacac actcaaagcc aaccctgccg
      421 ttgccggttt ggcaagcgag gaagaagact ctttcgtaag tgccggtgaa aacggacgct
      481 atctcgtcct attcgatcct ttagacggat cgtccaatat tgatgtcaac atttccgtcg
      541 gtacgatttt ctccatcctt gaaaaacccg aaggcgcatt ggcaaccgaa tcattccttc
      601 aaacgggcag acagcagctt gccgctggtt acgttcttta cgggcctcaa acccagctcg
      661 tattcacatt cggacacggc gtatatatgt tcacgctcaa tgccgaaaac gaatttgtgc
      721 tgacaaaaga aaaaccgaaa gtacccgaaa gtaccaaaga atttgccatc aatatgtcca
      781 accgccgcca ctggctgccc cccgtccaac aatacatcga cgagctcttg gcgggcgaaa
      841 ccggcacgcg cggcaaaaac tacaatatgc gctgggtagc cagcatggtt gccgaaatcc
      901 accgcatcct gatgcgcggc ggcgtgttca tgtatccgca agacaaacgc gaccccgcca
      961 agcccggcaa actgcgcctg atgtacgaag ccaaccctat gagcctgata ctcgaacagg
     1021 cgggcgcatc ggcaagcaac gcatatcaag ccatgctcga catacagccc gaaaacctgc
     1081 accaacgcgt cgccgtcatt atgggcagca gcgaagaagt ggattacctc aaccgcctgc
     1141 attcaaaata aaaggcagcc tgaccgataa agaaaggcgg cagcgcgcat ccgacgctgc
     1201 cgccgatatt tcgtttatag ccttaaaaag atagaaaacc acctttccga acggcaaaaa
     1261 tgccgtctga aaagttttca gacggcattt taaaaattaa agcccgaatt tatttgcccg
     1321 ctgctttttg caggatggcg tacaactcgt ctttcagttt caatttggct tttttcagct
     1381 catcgatggt ttccgcaccg ctggtaaccg gattgttgac cagaccggta attttatcgt
     1441 ccagctcgtt gtgttcgtcg aacagacggg cgaagcggga attttcctgt ttcaatttgg
     1501 aaatcaaatc acggtattct gggaacatat cgctttcctc tcttggttta cggttaaaaa
     1561 gtcaaattga atctttacat taattataac aaagataata tttttatcca gtgttttatc
     1621 ggatacggca aaattttctt ttaacaccgc cgtccggttg ataaaaacgt tgacaatatc
     1681 acgctttacg cataaatttt cagcctttcc tactgtatag tggattaaca aaaatcagg
//
LOCUS       DAWHXZ010000266         1710 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.266, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000266 DAWHXZ010000000
VERSION     DAWHXZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.266"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            173..952
                     /gene="pgeF"
                     /locus_tag="WIE35_001968"
     CDS             173..952
                     /gene="pgeF"
                     /locus_tag="WIE35_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ4154838.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDDPEAVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALGGTPDADAS
                     VDDTGKAACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            1004..1483
                     /locus_tag="WIE35_001969"
     CDS             1004..1483
                     /locus_tag="WIE35_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154839.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1485..>1710
                     /locus_tag="WIE35_001970"
     CDS             1485..>1710
                     /locus_tag="WIE35_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ4154840.1"
                     /translation="MAAHIGHIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLK"
BASE COUNT          391 a          487 c          489 g          343 t
ORIGIN      
        1 taagggcatc attcccgcac ctgcaagaat ccgaatccga acgtttgaac aattgaaaac
       61 aatcccgaat ctccgaattc ccgcctgtgc gggaatgacg aaaaaacaaa catccatttg
      121 ccccgaaggc agttaatcaa ccctttccgc cacacaccta ttccaatatc caatgaaaac
      181 catcacagaa accctaaatc tcgccccgaa aggcaaaaac ttcctgaccg ccgattggcc
      241 cgcgcccgcc aatgtgaaaa ccctgattac cacgcgcaac ggcggcgtga gccaaggtgc
      301 gtatcagagt ttgaacctcg gtacgcacgt cggcgacgac cctgaagccg tgcgccgtaa
      361 ccgcgaaatc gtgcaacagc aggtcggatt gcccgttgcc tacctcaatc aaatccacag
      421 caccatcgtc gtcaacgctg ccgaagcgtt aggcggcacg cccgatgccg acgcttccgt
      481 ggacgacacg ggcaaggccg cctgtgccgt gatgaccgca gactgtctgc ccgttctatt
      541 ttgcgacagg gcgggtacgg cggttgccgc cgcacacgcg ggctggcgcg gtttggcggg
      601 cggcgtattg caaaacacca ttgctgcgat gaaggttccg cctgtcgaaa tgatggcgta
      661 tctcggcccc gccatcagtg cggatgcgtt tgaagtcgga caggatgtgt ttgatgcgtt
      721 ctgcacgccc atgcccgaag ccgccgccgc atttgaaggc ataggcggcg gcaaattcct
      781 tgccgacctt tacgcgctcg cccgcctgat tctgaagcgt gaaggcgtgg gcggcgtata
      841 tggcggcacg cattgtacgg ttttggaacg ggacactttc ttttcctacc gccgcgacgg
      901 agcgacaggg cgtatggcga gcctgatttg gctggacggc aatgccgtct gaacacgccg
      961 ctgatataat ctaccgactt tgtgcttttg agaaaggcaa gccatgaaca aactgtttct
     1021 tactgccgca gtgctgatgc tgggcgcgtg cggtttccac ctgaaaggtg cagacggcat
     1081 ttctccgccg ctgacctacc ggagctggca catcgaaggc ggacaggcat tgcagtttcc
     1141 tttggaaacc gcgctgtatc aggcttcggg cagggtggac gatgctgccg gcgcgcagat
     1201 gaccctgcgt atagacagcg tttcccaaaa caaggaaacc tacaccgtta cccgtgcggc
     1261 agttatcaac gaatatcttt taatcttgac ggttgaagcg caggtattga aacgcggcga
     1321 gccggtcggc aaaccgatga ccgtgtccgt ccgccgcgtc cttgcttatg ccgacaacga
     1381 gatcttgggc aaacaggaag aggaagcggc attgtgggcg gaaatgcggc aggatgccgc
     1441 cgaacagatt gtccgccgcc tgacctttct gaaggcggaa tgacgtggcg gcacatatcg
     1501 gacacattga tacggacgcg cctttgaaac ccctgtacgt catccacggc gaggaagaac
     1561 tgttgcgtat cgaggcattg gacgcattga gggcggcggc gaagaaacaa ggttacctta
     1621 atcgggaagt ttatacggca gacaatgcct tcgattggaa cgaactgttg caaaccgcag
     1681 gcagtgcggg tctgtttgcc gatttgaagc
//
LOCUS       DAWHXZ010000267         1707 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.267, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000267 DAWHXZ010000000
VERSION     DAWHXZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.267"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..1142)
                     /locus_tag="WIE35_001971"
     CDS             complement(<1..1142)
                     /locus_tag="WIE35_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ4154841.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARYADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKGKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNPLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRA"
     gene            complement(1540..>1707)
                     /locus_tag="WIE35_001972"
     CDS             complement(1540..>1707)
                     /locus_tag="WIE35_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnD"
                     /protein_id="HEZ4154842.1"
                     /translation="VMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTWLFG
                     FKPRPGTPERA"
BASE COUNT          329 a          465 c          560 g          353 t
ORIGIN      
        1 gcgcgttttt cggtgatgac gcgcacggct tcgggttcgc ccaaatgcgg aaggatgcgt
       61 ttgaggtcgg cgtgaacttt gtccgcccac gctcggtttg taaacgcgcc gacgcggtcg
      121 gaaacgctga tgacggcgga cacttcgttt tcaggcagtc cgagcctgcc ccggcaaagc
      181 aaccattgtg ccgtgccgtc ggcaaggccg gtcagcgggg cgggcaggcg gacgggttcg
      241 gcgtagcgca gatagacggt ggtgatggcg tgatagcgaa ggttttgata tgccgtctga
      301 acgtgttcgg gcgtgccttc gggcaggagc gcggcggcgt ggtagggcgc ggtggcgggg
      361 acggcggcat cgaaagcttc gccgttgacg agcactttcc cgtccgggag ggggttcaga
      421 cggcatacgc gcgtttcgag gcggatgtcc gcgccgagcc gttgaagctc cgccaaggca
      481 ggttcggcga cgattgcgcc caaatcctgc ttgggtagga gatagtcgct gccggatttt
      541 ttcgtcagca cgccgtcgga caaaacgttg cacaacacgc gcaggcttgc ggtttccaaa
      601 ggcgtgttga gcgcacccca caccagcggc tgccaaaact gcatcacggc ggcacgcggc
      661 acgttccgct gtttcagcca ttgtgcaact gtcgtgtcgg gctgtccgag gcgtgcggac
      721 ttctgcaaat cggacatatc ggcaagcagt ttgcctttga atgcggacgg tacgcgccgg
      781 gcaagcagca cgccgcccaa aatatgcagc ggtgcgggca gggggagggc gcggaactgt
      841 aaaccgccgt gcatatgcca gtgcagcggt acgcgcaaaa aggcggcacg ggggtctgaa
      901 ccgatggttt tcatcaggcg caacacgccc cggtatgcgc cgagcaaaat gtgctgcccg
      961 ttgtccaaaa aaccgaaacc gtcggtattt ccggccagtg cgcgcgccct gccgcccgcc
     1021 tgccggccgg cttcaaacag ggtaacgtcg gcgtaccgcg ccaaggtgac ggcggcggac
     1081 agtcctgccc agcctgcgcc gatgacggcg attttcgggc gcggatgcgg cgtgttcatc
     1141 atttattcct ccaatggttt tgcagccgta tctatttccg tttctgaaaa tgacggtaga
     1201 aaaggataca ggctaaaaat aaaggcagca gaaagcgcgc atagggatat cacggatggt
     1261 ctgcatgcca gtatccgtac cgtccctgtg gaacggtatc atagcggtaa ttgtcgggga
     1321 aaaagttgat ccaaaccgtt actgccggcc ataaggctat atccctgact gctttcattt
     1381 gcttctgctc ctgtttaaat tcccggcaat tcgatttcaa agcgcgaacg ccaacgggat
     1441 tgcgcggtta cgatgcagac tttcagacga tggttgaaac cccgtccggc ggggctgtgg
     1501 gaatcgggtt tgcctgacgg gcggcgggcg gtatgcgcct tatgcccgtt ccggcgtgcc
     1561 ggggcgcggt ttgaatccga ataaccaggt tttcagggca atgcgttttt tgcgcggcga
     1621 agggagggcg attttgtatt tgaggacgtt ttgtgcgccg tcccggtcga tttcgttcaa
     1681 tagctcgtaa taaaccgccg ccataac
//
LOCUS       DAWHXZ010000268         1705 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.268, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000268 DAWHXZ010000000
VERSION     DAWHXZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.268"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..663
                     /gene="holA"
                     /locus_tag="WIE35_001973"
     CDS             <1..663
                     /gene="holA"
                     /locus_tag="WIE35_001973"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ4154843.1"
                     /translation="KLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIG
                     LGIEADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIDEAQTAVANVARFDVFQ
                     LAGAWMKGDVPRVCRLLDGLQEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVR
                     NGLRLWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEEGDAWTVFKRLVVSL
                     AE"
     gene            784..1203
                     /locus_tag="WIE35_001974"
     CDS             784..1203
                     /locus_tag="WIE35_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154844.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            1279..>1705
                     /locus_tag="WIE35_001975"
     CDS             1279..>1705
                     /locus_tag="WIE35_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ4154845.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLYLLRLRF
                     IWACIFIVFIPMLAYAGLRHLLGISPKDAFGAALIWLILP"
BASE COUNT          396 a          462 c          467 g          380 t
ORIGIN      
        1 aaactggaga aaacccagct ccagtccaaa tggtttgccg cattggcggc aaagggggaa
       61 gtgtgggaag ccaaaccggt cggcgcggcg gccttgcccc aatggatacg cggacggctg
      121 gacaaaatcg gtttgggtat cgaggcagac gcattggcac tgtttgccga gcgcgtggaa
      181 ggcaatttgt tggcggcgcg tcaggaaatc gacaagctcg cgctgctgta tccgaaaggg
      241 cataccgtca atatcgatga ggcgcaaacc gccgttgcca atgttgcccg ctttgacgtt
      301 tttcagcttg ccggcgcatg gatgaagggc gatgtcccgc gcgtatgcag gcttttggac
      361 ggattgcagg aggaaggcga agaaccggtg ctgcttctgt gggcggttgc cgaagacgtg
      421 cggacgctga tccggcttgc cgccgccttg aagcaggggc agagcatcca atccgtccgc
      481 aacggcctca ggctttgggg cgacaagcag acgctcgcac cgcttgcagt caagcggatt
      541 tccgtcgtcc gcctgcttga cgcgctcaaa acctgtgccc aaatcgaccg aatcatcaaa
      601 ggcgcggaag agggcgacgc atggacggta ttcaaacggc ttgtcgtgtc gctggcggaa
      661 taaagcggta atccccaaaa tccgaaaata ctgtaaaata ccgttaatcc tgaaaagtat
      721 tcaccaatcc gtccgaaaac atttcagacg gcacgaccac ctcaataaag gaacattaac
      781 cctatggaca ataagaccaa actgcgcttg ggcggcctga ttttactgac caccgccgtt
      841 ttaagcctca ttatcgtatt gattgtcgac tcctggccgc ttgccatcct gcttgccgcc
      901 gtcatcgtcg ccgccgctgc gggcggtttt gtttggacat cccgccgaca gcaacgccag
      961 tttatcgaac gcctgaaaaa attcgacatc gatcccgaaa aaggcagaat caacgaggca
     1021 aacctgcgcc gtatgtacca cagcggcgga caacaccaga aagatgcgat taccctgatc
     1081 tgcctgtcgc aaaaatgttc ggtggacgag gcgcacgcta tgttcaaaaa acgcccgaca
     1141 cgtcaggaaa tcaatcaaat ggcggcaaaa cagtcgcgcg gtcagaaacg tccgcaccgt
     1201 taaccgccgc aaggcatctt tgcataaatg ccgtctgaag cctgttggcg tttcagacgg
     1261 catattctga ttgaaaagat gatgacactg aaaaccgccc cgctcaaacg ccgctttgcc
     1321 gccatgctgt acgaaatgct gctggtcggt gcggcaacct gtttggcagc attgtttgcc
     1381 ggtattgccg ccatttttct gaatcccgtt tctatcgcgg tttctgcatt ggtaacaagt
     1441 atcctgataa tgggatcatg gtggctttat tttcgcgcca actggcacgg tcaggggcag
     1501 accttggcga tgaggacatg gaaaatcggc ttgtgcgacc ttaacggcat acagccgtct
     1561 ttgtacctgc tgcgcctgcg ctttatttgg gcgtgcatat tcatcgtctt cattcctatg
     1621 ttagcctatg ccggattacg ccacctgctc ggtatttcac ccaaggatgc attcggtgcg
     1681 gcattgattt ggctgatttt accgt
//
LOCUS       DAWHXZ010000269         1650 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.269, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000269 DAWHXZ010000000
VERSION     DAWHXZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.269"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            272..1576
                     /locus_tag="WIE35_001976"
     CDS             272..1576
                     /locus_tag="WIE35_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ4154846.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          319 a          423 c          507 g          401 t
ORIGIN      
        1 acctctgtat attttcctac agtaaattgt tgacaatcca tacgcccaca tatgcgccgc
       61 ctaaggataa atcctcccgc cggacaacgg gtgcaaggga ttggatgcga tatttccata
      121 ttcaaacaag ggatttgttt cacgcacagg gcggcacatc ggcaaaatcc ccgcgccgcc
      181 ccggtccggc agggcttgcg tccctcccgg acaagccccg accccgcctt tccgaaagac
      241 gggctcaacc attaaggaaa ctttaatcaa aatgaaaaaa cacatatggg cggcatcttt
      301 gctgccggca tccctatcgg cagaaccttt aaactggtgg aagccttatt ccgccgtcaa
      361 ttcgggcgat accgcctggg tgatgactgc ggctgccttg gtactgttga tgacgcttcc
      421 cgggctggct ttattctacg gcggtatggt gcggaaaaaa aacctgctct cgacgatgat
      481 gcacagcttt tccatcgcga cattggtggg aatcctttgg gtcgccgtcg gctattcttt
      541 agcgttcacg ccgggaaatg cctttatcgg cggtttgggg cgcgtatttt taagcgggat
      601 gcagatagac gctgccgcac agatgctgac cgtgtcgccc aatgcgccga ctgttcccga
      661 accggtattt atgttttttc agatgacgtt tgccattatt tcgaccgcca ttattaccgg
      721 cgcgtttgcc gaacggatga aatattcggc aatgatgctg ttttcgggca tatggttttt
      781 attggtttat gtgccgggcg cgcattgggt ctggggcggc ggctttatga gcaagggcgg
      841 cgtattggat tatgccggcg gtacggtggt gcacatcaat gccggtatcg cgggactcgt
      901 cgccgccttg gttttgggca ggcgcatagg ctacgggcgc gaggcgatgc ctccgcacaa
      961 tatggcgatg acactgatcg gcgcggcaat gttgtggttc ggctggttcg gctttaacgc
     1021 cggatcggcg cttgcggcag acgcggcggc gggtatggcg atggcggtaa cgcaggtgtc
     1081 ggccgtattc ggcgcggcag gctggcttgc ctgcgaaaaa atagcgggac acaaaccttc
     1141 cgctttgggg ctggcttccg gcgcggtttc cggtctggtc ggcatcaccc ctgccgccgg
     1201 ctttaccggc ccgtctggag cggccgccat cggtatattg actgccgccg cgtgctttgt
     1261 gtccgtcacc gtcgtcaaac acaaattgcg ttacgatgat tctttggacg ctttcggcat
     1321 acacggattc ggcgggctgg tgggcggaat attgaccggc atctttttcg acaaccgcat
     1381 tttcggcggg gatgcggcag tttggcagca gttgtggata caggtaaaag acgggttcgt
     1441 tatggcggca tacagcgggc tgatgagttg ggcgattttg aaggtcgtgg ggaaaatctg
     1501 cggcggcctg cgcgtcggca aggatgtcga acgcgaaggt ttggatctga atatccacgg
     1561 agaacgcgtg gaataagggc ggctatgccg tctgaagcct gaaaatcggg tttcagacgg
     1621 catttttcac gtttgccgcc gatggataaa
//
LOCUS       DAWHXZ010000270         1650 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.270, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000270 DAWHXZ010000000
VERSION     DAWHXZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.270"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..795)
                     /gene="dacB"
                     /locus_tag="WIE35_001977"
     CDS             complement(<1..795)
                     /gene="dacB"
                     /locus_tag="WIE35_001977"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ4154847.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRIS"
     gene            960..1526
                     /locus_tag="WIE35_001978"
     CDS             960..1526
                     /locus_tag="WIE35_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ4154848.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          391 a          376 c          490 g          393 t
ORIGIN      
        1 tgaaatccgt ccgccgccga gcagccagtg gttggtaaaa ctttgccgga tcagttcgtc
       61 aagcgcgaac atccggacac cgacaggctt gcccaaacag ctctcgggaa tattgccgcg
      121 caatttcagc gtattgtccg aaaaagatgc acgcatcagt tttttgatcg aagggcaggc
      181 agcttgggag gcggtaattt tcaagttgtt ttgggcgaaa atatgcggca aaggcggatc
      241 ggtgaggatg tcggtactgt cggcggcatt gcgttcggcg cgcaccataa ccataccggc
      301 agacagcata gttggattgg ggggcgtcat aaacggcgaa ccgctgtcgg cttcgaaatc
      361 gtcggggctg ccgacttcgc cccacaggct gtggtcgagc atcaggtgtc ccgtgatatt
      421 gcgtatgcct tgttcgcgca actggcgttg gacggcaagc aggttttcct gattgaaaac
      481 ggggtcgccg ctgcccgccc aatacaggtt tccgtcaagc gtgccgtcgt ttaccgtacc
      541 gttgctttta aactcggtcg cccagcggta attgctgccg aaggttttga aggcggcaaa
      601 cgcggtaacg agtttcattg tggaggcggg gttgacgggg acatccgagc ggtggtcaat
      661 gatgactttt ccgctgtcaa gctcttggac atatacggcg atttcgtttt gcggaatgcg
      721 gccggtatcg agcgcgtgtg cggcgaggga ggcgagaagc agcagcaggg aggccgctgt
      781 tttggggaaa ttcataggta aatctgttac ataaaaaaag caattataag gcaaagccgg
      841 ataagtggga acgctgtggc ggcgggccgg cttgtttgca ggggaaatca catatataat
      901 aatcgctatc attatgaaat gattgagacg cacaaactta atattcagga ggaatgatta
      961 tggctaagaa aatcagtatt ttggtgggca gcctgcgccg tgcttctttt gcgcgcaaag
     1021 tggcattgaa tgcggcggag atgttccccg aaggctggca ggcggaaatc gtcgaaatcg
     1081 gacatctgcc gctttacaat ttcgattatg acgaccctgc ggtggaagat gtgccgctgc
     1141 ccgaaagcta cacggctttc cgcgaaacga ttaaggcttc ggacggcatt ttgttcgtta
     1201 cgtccgaaaa taaccgcacc attcccgcct gtttgaaaaa tgcggtggac atcggctcga
     1261 aaccgaatgc cgacgtggcg tggaaaaaca aaccggccgg tatcatcagc cattccgtcg
     1321 gcaagatggg cggttacagt tcgcaaaaaa acctgcgcct tgcgctgtct tatttcgata
     1381 tgcccgtaac cggacagccg gaagtgtttt tgggcaattc gccgacgctg tttgatgaaa
     1441 acggcaaatt gatcgactcg gcaagggatt ttgttcagtc atatattaat cagtttgttg
     1501 gtttgattga aagaaatgcc aaataaacaa accaagtcga aaggccgcaa aaccggttcg
     1561 ggttttgcgg cttttttata tgggctgggc agcgtcaagg tttgccgttt gccggataat
     1621 gcgcggcaaa tgaccggcgt gtttatagcg
//
LOCUS       DAWHXZ010000271         1641 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.271, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000271 DAWHXZ010000000
VERSION     DAWHXZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.271"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            171..1622
                     /gene="cobA"
                     /locus_tag="WIE35_001979"
     CDS             171..1622
                     /gene="cobA"
                     /locus_tag="WIE35_001979"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ4154849.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGD
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENGEGENRVGQTYPALGGLNAGQRAA"
BASE COUNT          362 a          510 c          477 g          292 t
ORIGIN      
        1 aaaccgccgt atccgcagcc gccctcatat agctttcagc ttctaacaaa tgccttttta
       61 ttatttaacc gggaaaatcc ttttgtctaa aatgacccgc attccgacaa aaatgccgcg
      121 acgcaccgta tcggaaacaa ggtgcgggca aacagcaaag gagtcagcca gtgaattatt
      181 tccctatttt cgccaacctc gccggccgcc ccgtattggt cgtaggcggc ggcgcggtcg
      241 ccgcacgcaa aatcagcctg ctgctgaagg cgggcgcaga ggtcagggtt gccgcaaaac
      301 acctgaatgc cgaactctcc gcgttggcgg cggaaaacaa aatcctgtgg cttgccgaag
      361 aatttcgtgc cgaacacatc cgcacggttt tcctcatcat tgcggcaagc agcgaccaag
      421 ccctcaaccg gcgcgttttc catcttgccg aaagctgcca aaagccggtc aacgtggtgg
      481 acgaccgcga ccattgcagc ttcatcttcc cgtcggttat cgaccgcaac ccggttcaga
      541 ttgccgtttc cagctccggc agcgcgcccg tcctcgcccg actgctgcgt gaaaggctgg
      601 aagccctgct gccgccgtct ttgggcgata tggcggaaat ttcaggaagg tggcgcgatg
      661 ccgtcaaggg caaactgaaa tccgttaccg aacgccgccg cttttgggaa aagcagttta
      721 acggacgttt cgccgccctc gtcaaaaacc ggcaaaacac ccttgccgaa cgggaattgg
      781 caggccaatt ggaacaaagt cgtcaaaatg atcaaggcgg ctccgtctcg ctggtcggcg
      841 cgggtcccgg cgatgcgggg ctgctgacgc tcaaagggct gcaggaaatc cagcaggcag
      901 acgtggtgct ttacgacgcg ctggtttcag acggcatact gtccctcgtc cgccgcgatg
      961 cggaacggat ttttgtcggc aagcgcgccc ggggcgaccg caccccgcaa gaggacacca
     1021 atgcgcttat ggtcaggctg gcccgcgaag gcaggcgcgt ggtgcgtttg aaaggcggag
     1081 accctttcgt gttcggacgc ggcggcgaag aattggaaac cttggcacgc catcagatcc
     1141 cgttttcggt cgtccccggc attaccgccg ccgtcggcgc gaccgcctat gccggcatcc
     1201 cgctcacgca ccgcgattat gcccaaagtg cggttttcgt aaccggccac cgcaaggcgg
     1261 atgcgcccga catcgaatgg cagaccctcg cccgcagccg ccagaccttg gtgatttata
     1321 tgggtgcgct caaagccgcc ctgattgccg aacggctgca acagcacgga cgctcgcccg
     1381 acacgccagc agccgtcatc agccaaggca cgctgccggc tcaaaaaacc gcaaccggca
     1441 cgctcgccaa ccttgccgaa ctcgccgaaa ccgcgccgaa tccggcattg attgtcatag
     1501 gcgaagtggt ggggctgcac gaaaaactcg cctggttcgg tgaaaacggc gaaggagaaa
     1561 accgggtcgg gcagacgtat cccgcattgg gcggactgaa tgccggacaa cgggcggcat
     1621 aaggcgggaa aaatggaaac g
//
LOCUS       DAWHXZ010000272         1545 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.272, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000272 DAWHXZ010000000
VERSION     DAWHXZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.272"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            37..1410
                     /locus_tag="WIE35_001980"
     CDS             37..1410
                     /locus_tag="WIE35_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ4154850.1"
                     /translation="MSNPFSSLGLGTELVSALALQGYETPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNIHLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGNLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT          392 a          509 c          388 g          256 t
ORIGIN      
        1 tcagacggca taaggtctga aagaaagact acaattatga gcaacccatt ttcctcttta
       61 ggtttgggta cggaactggt ttccgcactc gcgcttcaag gctatgaaac cccgaccccc
      121 attcaggccg cagccattcc gaaggcgctg gccggacacg acctgcttgc cgccgcgcaa
      181 accggcacgg gcaaaaccgc cgcctttatg ctgccctcgc tcgaacgcct caaacgttac
      241 gccaccgcca gcacctcgcc cgcgatgcac cccgtgcgta tgctcgtcct cacccccacg
      301 cgcgaacttg ccgaccaaat cgaccaaaac gtgcagggct acattaaaaa tctgccgctg
      361 cggcataccg tcttgttcgg cggtatgaat atggacaaac agaccgccga tttgcgcgcc
      421 ggctgcgaaa tcgtcgtcgc caccgtcgga cggctgctcg accacgtgaa acagaaaaac
      481 atccatttga acaaagtcga aatcgtcgtt ttggacgaag ccgaccgtat gctggatatg
      541 ggttttatcg acgacatccg caaaatcatg cagatgctgc cccgccaacg ccaaaccctg
      601 ctcttttccg ccaccttctc cgccccgata cgcaaactgg cgcaagactt catgaacgcg
      661 cccgaaaccg tcgaagtcgc cgcgcaaaac accaccaacg ccaacgtcga gcagcacatc
      721 atcgccgtcg ataccattca gaagcgcaac ctgctcgaac ggctgattgt cgatttgcat
      781 atgaaccagg tcatcgtgtt ctgcaaaacc aaacaaagcg tcgaccgcgt aacgcgcgaa
      841 ctggtgcgcc gcaacctgtc cgcacaggcg atacacggcg accgttccca acaaagccgg
      901 ctcgaaacac tcaacgcctt caaagacggc aacctgcgcg tcctcgtcgc caccgatatc
      961 gccgcgcggg ggctggatat tgccgaactg cccttcgtca tcaattacga aatgcccgcc
     1021 cagcccgaag actacgtcca ccgcatcggg cgcacggggc gcgcgggcgc ggacggcgtg
     1081 gcgatttccc tgatggacga atccgaacag aaaatgtttg aatccattaa agagctgacc
     1141 ggcaacaagc tgctcatcga gcgcatcgag ggcttcgagc cgcaatggtg ggaacagggc
     1201 ggcgcaaaac cggaaaaacc cgaaatgcgc gaaccaagac aacgcaaccg ctacgaatcc
     1261 gccaaagcgc aacgcgaaaa aaacacccgg ccggaaaatg cggcaaacga tgcgggcgcg
     1321 gcttgcggaa aaattgccgg acgcagccgc cgaagccgcc gggaacaccg gacgtgcgcc
     1381 ctgctccaac cgcgttacgg cgtaaaataa ccctgaaaat caaatgccgt ctgaacattt
     1441 cccgtttcag acggcatttt tcaaaccgga ctgacgcatt gggcgcaacc gcccgcaccg
     1501 gataaatttc tgccgcaaac agtttcagac ggcatttgcc gcctg
//
LOCUS       DAWHXZ010000273         1529 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.273, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000273 DAWHXZ010000000
VERSION     DAWHXZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.273"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..707
                     /locus_tag="WIE35_001981"
     CDS             <1..707
                     /locus_tag="WIE35_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEZ4154851.1"
                     /translation="ASDGISIHNFPKGTQAGLCLHEILEDFKFGQAAAGQETLITDKL
                     KKYGFEEIWLPAVAEMAEACRKTPLTGAYGLSDIPPECRCPEMGFTLHTEDFGLKRLR
                     DWFARDDIRLPEVCRAAAETLDFHTVNGFLNGFIDMVCQDPEGNICVIDYKSNHLGAD
                     ASAYTRQAMDEAVAHHHYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGG
                     GVWRWDIDAAALEQIK"
     gene            746..1105
                     /locus_tag="WIE35_001982"
     CDS             746..1105
                     /locus_tag="WIE35_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ4154852.1"
                     /translation="MNIKHLITTALIASAAFAAQAAPQKPLSAAQTAQHPVVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            1230..>1529
                     /locus_tag="WIE35_001983"
     CDS             1230..>1529
                     /locus_tag="WIE35_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ4154853.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPQTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVK"
BASE COUNT          394 a          498 c          374 g          263 t
ORIGIN      
        1 cggcttcaga tggcatatcg atacacaact ttccgaaagg cacgcaggcg gggctgtgcc
       61 tgcacgaaat tcttgaagat ttcaaattcg gacaagcagc cgccggacag gaaaccctca
      121 tcaccgacaa gctgaaaaaa tacggatttg aagaaatatg gctgcccgcc gttgccgaaa
      181 tggcggaagc ctgccgcaaa acgccgctga cgggggcata cggcctgtcc gacatcccgc
      241 ccgagtgccg ctgtcccgaa atgggcttta ccctccacac cgaagacttc ggcctcaaac
      301 ggctacgcga ttggtttgcc cgcgacgaca tcaggctgcc cgaagtctgc cgtgccgctg
      361 ccgaaacgct agacttccac accgtcaacg gctttttaaa cggctttatc gacatggtct
      421 gccaagaccc cgagggaaat atctgcgtca tcgactacaa atcaaaccat ctcggcgcgg
      481 acgcatccgc ctacacgcgg caggcgatgg acgaagccgt cgcgcaccac cactattacc
      541 ttcaggcgct gatttacgcc gttgccgccg cacgctactt caaactgcgc ggacaaccgc
      601 ccgccgccgt ttccgtccgc tacctgtttt tgcgcggatt ggatggcaaa ggcggcggcg
      661 tttggcgttg ggacatagat gccgccgctt tggaacagat aaaataactt cccgtccttt
      721 cggacaacaa ccgcaggaga accctatgaa tatcaaacac ctgattacca ccgcactcat
      781 tgcctcagcc gcctttgccg cgcaggcagc cccgcaaaaa cccctatccg ccgcccaaac
      841 cgcgcaacat ccggtcgttt ggatcgatgt ccgttccgaa caggaattta gcgaagggca
      901 tttgcacaac gcggtcaaca tccccgtcga ccaaatcgtc cgccgcatac acgaagccgc
      961 gcccgacaaa gacacgccgg tcaacctcta ctgccgcagc ggacggcgtg ccgaagccgc
     1021 ccttcaagaa ctgaaaaaag caggttatac aaatgttgcc aatcacggcg gttatgaaga
     1081 cctgctcaaa aaagggatga aatgacgcaa tgccgtctga aaggacaggc tttcagacgg
     1141 catacggcgt tccggcacgc ggcgcaacat cgggcgcggc gcgcgccgcc ccgtccgaag
     1201 cctgataaac aaaccgaaaa ggaaatacga tgaaacacat actccccctg attgccgcat
     1261 ccgcactctg catttcaacc gcttcggcac atcctgccag cgaaccgcaa acccaaaacg
     1321 aaaccgctat gaccacgcat accctcacct caaaatacag ttttgacgaa accgtcagcc
     1381 gccttgaaac cgccataaaa agcaaaggga tggacatttt tgccgtcatc gaccatcagg
     1441 aagccgcccg ccgaaacggc ttaacgatgc agccggcaaa agtcatcgtc ttcggcacgc
     1501 ccaaagccgg cacgccgctg atggtcaaa
//
LOCUS       DAWHXZ010000274         1518 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.274, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000274 DAWHXZ010000000
VERSION     DAWHXZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.274"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(270..>1518)
                     /locus_tag="WIE35_001984"
     CDS             complement(270..>1518)
                     /locus_tag="WIE35_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ4154854.1"
                     /translation="PKGNGYKSLHTVIVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRY
                     KEGGKGDSAYEQKIAWLRQLLDWRENMAESGKEDLAAAFKTELFNDTIYVLTPHGKVL
                     SLPTGATPIDFAYALHSSIGDRCRGAKVEGQIVPLSTPLENGQRVEIITAKEGHPSVN
                     WLYEGWVKSNKAIGKIRAYIRQQNADTVREEGRVQLDKQLAKLTPKPNLQELAENLGY
                     KKPEDLYTAVGQGEISNRAIQKACGTLNEPPPVPVSETTIVKQSKIKKGGKNGVLIDG
                     EDGLMTTLAKCCKPAPPDDIVGFVTRDRGISVHRKTCPSFRHLAEHAPEKVLDASWAA
                     LQEGQVFAVDIEIRAQDRSGLLRDVSDALARHKLNVTAVQTQSRDLEASMRFTLEVKQ
                     VNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          262 a          364 c          472 g          420 t
ORIGIN      
        1 gtggattaaa tttaaaccag tacggtgttg cctcgccttg ccgtactatt tgtactgtct
       61 gcggcttcgt cgccttgtcc tgatttttgt taatccacta tagtttaaag ctttgtttct
      121 taagtccgca gagtatgcca tggttagacc ttcaaagttg agtattgtac tattttgttt
      181 ttaattgact atgcaacaaa aaatagcaaa ccccgtcaat caaaatgccg tctgaagcgt
      241 tatacggctt tcagacggca tttttgtatt taaagccggg taacgctcaa tacgcctttg
      301 acgtcgccga ggctggcgag gacgcgcggg aggtcgttga cctgtttgac ttcgagcgtg
      361 aaccgcatac tggcttccaa gtcgcgggac tgggtttgca cggcggtaac gttgagtttg
      421 tggcgggcga gcgcgtcgga tacgtcgcgc aaaagcccgg agcggtcttg ggcgcggatt
      481 tcgatatcga cggcgaacac ttgtccttcc tgcaacgccg cccaacttgc gtccagtact
      541 ttttcgggcg cgtgttcggc gaggtgtcgg aaagaggggc aggttttgcg gtgtaccgaa
      601 atgccgcgat cgcgggtaac gaagccgaca atgtcgtcgg gcggcgcggg tttgcagcat
      661 ttggcaagcg tggtcatcag accgtcttcg ccgtcgatga gcacgccgtt tttgccgcct
      721 tttttgattt tggactgttt gacgatggtg gtttcgctga cgggtacggg cggcggttcg
      781 ttcagcgtgc cgcaggcttt ttggatggcg cggttggaaa tttcgccttg tccgacggcg
      841 gtgtagaggt cttctggctt tttgtagccg agattttcgg caagctcttg caggttgggt
      901 ttgggcgtga gtttggcaag ctgtttgtcg agttggacgc ggccttcttc gcgcacggtg
      961 tcggcgtttt gctggcggat gtaggcgcgg attttgccga ttgccttgtt ggatttgacc
     1021 cagccttcgt aaagccagtt gacggaagga tgcccttctt tggcggtaat gatttcgacg
     1081 cgctgtccgt tttcgagcgg ggtggacagc ggcacaatct gcccttcgac tttcgcaccg
     1141 cggcaacggt cgccgatgct gctgtgcagg gcgtaggcga agtcgatggg ggtcgcgccc
     1201 gtgggcaggg agaggacttt gccgtgcggg gtcaggacat aaatcgtgtc gttgaaaagc
     1261 tcggttttga aggcggcggc gaggtcttcc ttgccgcttt ccgccatgtt ttcgcgccag
     1321 tccaagagtt ggcgcaacca agcgattttc tgttcgtagg cggaatcgcc tttgccgccc
     1381 tctttataac gccagtgggc ggcaacacca aattcgttga attggtgcat atcgaaggtg
     1441 cggatttgca cttccacgcc tttgtcttcc gggccgacga taacggtgtg caaactttta
     1501 tagccgttgc ctttcggg
//
LOCUS       DAWHXZ010000275         1466 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.275, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000275 DAWHXZ010000000
VERSION     DAWHXZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.275"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..661)
                     /locus_tag="WIE35_001985"
     CDS             complement(<1..661)
                     /locus_tag="WIE35_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ4154855.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDNEGRKSYAVASESVA
                     FN"
     gene            complement(869..1366)
                     /locus_tag="WIE35_001986"
     CDS             complement(869..1366)
                     /locus_tag="WIE35_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ4154856.1"
                     /translation="MNNLPVADLLVFAIIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLVMLASKTDLPDTEEWQQSYTIPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            complement(1359..>1466)
                     /locus_tag="WIE35_001987"
     CDS             complement(1359..>1466)
                     /locus_tag="WIE35_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ4154857.1"
                     /translation="KVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
BASE COUNT          358 a          364 c          432 g          312 t
ORIGIN      
        1 cgttgaaggc gacggattcg gaggcgacgg cataggattt cctgccttcg ttgtcggttt
       61 gcgaacccaa taccagcggg cggatgccgt aagggtcgcg gaaggcaagc ataccgtagc
      121 ccgcaatcat ggcaaccacg ccgtatgcgc cgcgcactag gcggtggact tcggcaacgg
      181 cgttgaaaat attgtcggca ttgagccggt gcggatcggc gtttttggag acttcgcggc
      241 gtaattcgtg cgcgaatacg ttgagcaaga cttcggaatc ggagctggtg ttgacgtggc
      301 gcaggtgttt gttgcacacg ttttcataca gttcggccgt gttggtgaga ttgccgttat
      361 gcgccaaaac gatgccgaaa ggcgagctga cgtagaaagg ctgcgcctcc gcgctgctgc
      421 cggcgttgcc cgccgtagga taacggacgt gggcgatgcc ggcgttgccg accaaatcgc
      481 gcatattgcg cgtgcggaac acttcgcgca ccatcccttt gcctttgtgc atatggaagg
      541 tgccgccttc cgccgttgca atgcccgccg catcctgccc cctgtgctgc aacatctgca
      601 aaccgtcgta cagaagctgg ttcacgggtt catgactgac caaacctaat acgccgcaca
      661 tatcgtcttc tccgattcga ggtttaaggg taaaacggaa ttataaagca aacggtggtt
      721 ttttgcctga attgttgaca atatttgagc gaaggacaga taggtgggtt tatggaggat
      781 aagatttaat ctgtgatatg gctgagaaaa ggaaaaaaca tttcagacgg cataaaagag
      841 aatgccgtct gaaatatccg tatggcaatc aatcgtcttc cggagtttcc gccgtgccgc
      901 cgctatggtt caacacggct tcggaaagcg atacgaaaaa cggtatggta taggactgct
      961 gccactcttc ggtatcgggc aggtcggttt ttgaagcaag catgaccagc agggtaacaa
     1021 tcaaaacgcc tttcaatgca ccgaatacgc cgcccaaaat gcggttggca aagcccaaac
     1081 cgaccgccga aactgcgccg gtcagcagcg aacggagcat tttctggatc agacaggcaa
     1141 tgacgaacag ggaaatgaac gacagagcca atgcaaacag gcggggttgg aacgaggcaa
     1201 aggcgaggtc ggcgaagggt gcggcaaaga gtttggcaaa aaagaaggaa accacccatg
     1261 ccgccatcga acctgcctcc gcaatcacgc cgcgcatcgc ggatagcacg atgcaggcgg
     1321 cgatgatggc gaagacgagg aggtcggcaa cggggaggtt attcattcgt tacctgaccg
     1381 gcgataccgt gtacacgcaa tttgttcaaa tcgcgttcgg catcccttgc gtttttatag
     1441 ttgcttgatt tgacgcggta aacttt
//
LOCUS       DAWHXZ010000276         1444 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.276, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000276 DAWHXZ010000000
VERSION     DAWHXZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.276"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            27..1364
                     /locus_tag="WIE35_001988"
     CDS             27..1364
                     /locus_tag="WIE35_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ4154858.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYLISAVIMPVGALSVA
                     VFTAWIQDRQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT          263 a          340 c          389 g          452 t
ORIGIN      
        1 tatcttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
       61 gtttcgtgct tgctgcggca ggttcggcca tcggtttggg cgcgatttgg aaatttcctt
      121 atacggcagg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttaccgtct
      181 tggtcgccct gcccgtccag cttgccgagt tttatatcgg gcgcacaggc ggtaaaaatg
      241 ccgtcgattc cttcagggct ctgcgtccga gtacgcaatg gctttgggtc gggcgcatgg
      301 gcgtttccgc ctgctttatt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattaa
      361 attatgtcgt ccacagtttc acgggggcgg ttcataccgg cgcggacttt gaagccttgt
      421 tcggcgcgac gatttccaat ccggcaggtt cgctgtccta tcaggcactg tttatgctga
      481 ttacggtttg ggtggtcaaa ggcggcattt cagacggcat tgaaaaggca aaccgttatc
      541 tgatgccggg gctgtttatc ctctttattg cgctggcaat ccgttcgctg acgctgccgg
      601 gtgcaatgga gggcgtgtct ttcctgctca aaccgaattg gtcgtacttt aaagccgata
      661 cgatgattac ggctttaggt caggcgtttt ttgccttgag catcggcgtt tccgctatga
      721 ttacctatgc ttcgtatttg ggaaaagatc aggatatgtt ccgctcgggg catacgatta
      781 tgtggatgaa cctcttggtt tcgctgttgg caggtttggt gatttttccg gcggtgttcg
      841 ccttcggttt tgaaccgagc caggggccgg gattgatttt tatcgtattg ccggcggtgt
      901 ttatgaaaat gccgttcggt acggttttgt ttgcggtatt tatgctgctg gtcgttttcg
      961 ccacgctgac ttcggcattt tcgatgttgg aaacggtcat cgccgcaacc atccgccaag
     1021 acgagcgcaa acgcaaaaaa catacttggc ttatcggcac ggctattttt attgtcggca
     1081 tcccgtccgc gctgtctttc ggcgtatggg gtgagtttaa ggttttcggc aaaaccattt
     1141 ttgatttgtg ggactacctt atttctgcag tcatcatgcc tgtcggcgca ttgagcgttg
     1201 ccgtatttac cgcttggatt caggacaggc agtctgtgtt aaaagatgcc ggcgcgggca
     1261 gcaccgtacc acgggcagtg ttgctgctgt ggctgaatac cttgcgctac ctcgccccga
     1321 ttgccattat tattgttttc atcaattctt tggacatcct ttaaaagcca tccaaacagc
     1381 aaaaatgccg tctgaaagcc tttcagacgg catttttgct tcaggttcag cctatttcgt
     1441 tcaa
//
LOCUS       DAWHXZ010000277         1436 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.277, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000277 DAWHXZ010000000
VERSION     DAWHXZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.277"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(183..1172)
                     /gene="porB"
                     /locus_tag="WIE35_001989"
     CDS             complement(183..1172)
                     /gene="porB"
                     /locus_tag="WIE35_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ4154859.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVAHNGAQA
                     ASVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFMQYGGAYKRHQNVDNVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVKDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFDD
                     ADYTNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGESKFVSTAGGVGLRHKF"
BASE COUNT          353 a          326 c          359 g          398 t
ORIGIN      
        1 tcttaaccct gagtcctaga ttcccgcctg cgcgggaatg acggcatatt ttgacattga
       61 ataaaaaaga ctaaaaacag gaaaaagcca aacagaaaac agcgaaacag aaaacaggaa
      121 aaagccaaac agaaaaaagc ctgtctggcg acaggctttt tgttgatacc aatctttgca
      181 gattagaatt tgtggcgcag accgacaccg ccggcagtcg atacgaattt gctttcgcct
      241 ttgccttctt gcaaccaacc ggcagaaacc aaggcagaag tgcgtttgga gaagtcgtat
      301 tccgcaccga caaccacttg gtcgtaatcg ttggtgtagt ctgcatcatc aaacgagcct
      361 ttgaagccgt gggcgtaaga aacgcggggc gttacgttgc cgaagcggta tgccaaggta
      421 gcggcaactt cggtttgaga gttgtgcgaa ttgtctttaa ccagtttcgc gtcttgttgc
      481 tgtacggcta cggaagcgta cagggcatca ttgtcgtaac cgctgaccaa acggtgaatc
      541 tggtatttct caatcttcac gttatccaca ttctgatgtc ttttataggc accgccatat
      601 tgcatgaaga agccgccgtt tttgtagttg aagccggcgt ggtaagattc gctgttatgt
      661 ctgcctgcat tgtcgttaag cgcgtattgt acgctgccgc tgaggccggc aaattcggga
      721 gaatcgtagc gtacggaaat gaggcgtgct tcgggttcgg caattttgtt tacacccaaa
      781 tagtcgcttt tgctatccca aggattgatg tcgccggtgt ctttcaggac gctgttcaaa
      841 cggccgacgc gcaatttacc gaagccacct ttcaaaccga tgaaggattg gcggttgccc
      901 caaccggagt cagtaccggc gatagatgcc ttttgctcaa cctgccaaat ggctttcaga
      961 ccgttaccga ggtcttcttg gcctttgaag ccgattttcg aacccaaatc aacgatgccg
     1021 gtaccggttt caacgctagc cgcctgagct ccattgtgag ctacggagcg ggaagtttct
     1081 acgccggctt tgatggtgcc gtacagggta acgtcagcca ttgctgcaac aggaagggct
     1141 gccaaagtca gggcaatcag ggattttttc attgctgtat tccttttttg gttaagaaat
     1201 ttaagccggc cgggctttcc aagccgctta gctttgcatt taccgccgac gttttcggcg
     1261 attccatgtc tgaaactata gatgttcctg tctgaaaaaa caaacttctg cctgtttttg
     1321 tgtggaattc gatgtgtatt ttgaagggcg gatagagata ttatgggtat tttttgtttt
     1381 ataacatatg gttatttaaa ttttttaaga tttgcatttt tacaacactt actcgg
//
LOCUS       DAWHXZ010000278         1430 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.278, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000278 DAWHXZ010000000
VERSION     DAWHXZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.278"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(34..492)
                     /locus_tag="WIE35_001990"
     CDS             complement(34..492)
                     /locus_tag="WIE35_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154860.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(1056..1259)
                     /locus_tag="WIE35_001991"
     CDS             complement(1056..1259)
                     /locus_tag="WIE35_001991"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154861.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
BASE COUNT          490 a          263 c          176 g          501 t
ORIGIN      
        1 cgatcgattc cgttgtattt tcgcaagacg cgtttagtct agagtctgta tattacattt
       61 atttttaggg tctgctagcc aatttcttgt tcccttcatt attttatctt ctgaaagaaa
      121 attatttttt tccatgctat taatattaat gatatgattt ttatttaaaa taatttttcc
      181 atataaaatt tccggtttgt aatagcaaga gtctgaagca aatccttgcc cgtcaatata
      241 agaaaaataa atgttatcat cctccttata atattctaaa accaatccgg atattatttt
      301 atcaaaattc ccttcatttc catctttcat gactaaaata taattagcag gccaaccttt
      361 atcgtaaaca tattcaaact gatagttccc gaaactctcg atatccgaac taaaaaagaa
      421 gaaaagcaaa ttaatagcca atacaaaaac aaataagaac aaaaatagca aaattttcaa
      481 cttagttaac aatatttacc tctcctttaa attcaatcct gaaaggtacc ccttaccctg
      541 ggctaccaat tatagtttcc atatttctaa atattgtttt tacattactt ttttctcccc
      601 ccaaaggaat gcattttaaa atcatgcttt tcaggtgcta atcgatactt accattaaca
      661 tctttaatca cagatatatt tccatgtata gcccaacgtg aaaaatctga gtattatata
      721 cagttatacc ttactgaaac aaagctagct gtacatatct gtaccatatt tttactacaa
      781 acaaaaaata tttataatta ttaaggataa gataaaaacc cccaacgcta gattaattct
      841 taataatttc aaataaaaaa tatttgagat tttttctttt ccaaataata tattgaaata
      901 tttaaagtta aggtagcttg atttaagtag aaaatcaaaa aaaccattaa ttaatttttc
      961 ttctttaaat attttatatg atattataaa attaaataac attgcgaaaa catatgaaat
     1021 catcaaaata ccaaatatat agtgggaatg cattattatt tagctccttc ttttggctct
     1081 gacaaattga cagtccattg atatccaatg cttgcatttg caattgctga cgcacctgcc
     1141 cctgttttcc ctgttagtat caatggttct tttcttgcaa gatgtgcacc tgcacaacca
     1201 cctaatacta aaccaccacc tattgtaaat ccttcctgtt gtatctctaa tgataacatt
     1261 ttcttcttgt gccgccctta atacagattg tggaattgga ttgacctgag caggaacttc
     1321 taaaataagt tttccttcaa gctgtcttcc tcttagcgaa gcaggtgtac ttggaacatt
     1381 ggaaatgaca aagtgagtgc gcaaagccac ctgaaggcga tgtgtttgaa
//
LOCUS       DAWHXZ010000279         1404 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.279, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000279 DAWHXZ010000000
VERSION     DAWHXZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.279"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..203
                     /locus_tag="WIE35_001992"
     CDS             <1..203
                     /locus_tag="WIE35_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HEZ4154862.1"
                     /translation="RDGYWHLPQTPARKDLTDIESKKRSMYRKRYEMLDNMVAEMKDG
                     LKTEARGISDGIQTEKPLRRTA"
     gene            246..>1404
                     /gene="gatB"
                     /locus_tag="WIE35_001993"
     CDS             246..>1404
                     /gene="gatB"
                     /locus_tag="WIE35_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ4154863.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSK"
BASE COUNT          402 a          397 c          363 g          242 t
ORIGIN      
        1 aacgggacgg ctactggcat ctcccccaaa cccccgcccg caaagacctt accgacatcg
       61 aaagcaaaaa acgctcgatg taccgcaagc gttatgaaat gctggacaac atggttgcag
      121 agatgaagga cggtctgaaa acagaagcac gcggcatttc agacggcatc caaacggaaa
      181 aaccgctccg ccggacagcc tgacgcgaag actatcgaat tgatatttta gagaaagaag
      241 ctcttatgac ctgggaaacc gtaatcggct tggaaatcca cgtccaattg aacaccaaat
      301 ccaaaatctt cagcggcgca tcgaccgcat tcggcgcaga acccaacgcg cacgccggcg
      361 tagtggaatg tgctttgccg ggcgtactgc cggtaatgaa ccgagaagtc gttgaaaaag
      421 ccatcaaatt aggcttggct ttggatgcga aaatcaaccg gaaaaacgtg ttcgaccgta
      481 aaaactactt ctatcccgac ttaccaaaag gctatcaaat cagccagttg gacttaccga
      541 ttgtcgaaca cggcaaattg gaaatcgtag tcggcgacga tgtgaaaacc atcaacgtaa
      601 cccgcgccca tatggaagaa gacgcgggca agtccgtaca tgaaggcttg aacggcgcga
      661 ccggtatcga tttgaaccgc gccggcacgc ctttattgga agtcgtatcc gaacccgaaa
      721 tgcgctccgc cgccgaagcc gttgcctacg ccaaagcctt gcacagcttg gtaacctggc
      781 tgaacatttg cgacggcaat atggcggaag gttcgttccg cgtcgatgcc aacgtatccg
      841 ttcgcccgaa aggtcaagcg gaattcggca cgcgccgcga gattaaaaac ctcaattcct
      901 tccgtttctt ggagcaggcg attaattacg aagcagaagc gcaaatcgag attttggaag
      961 acggcggcaa agtacagcag gcaacgatgc tgtttgatcc cgaaaaaggc gaaacccgcg
     1021 taatgcgcct gaaagaagac gcgcaagact accgctactt ccccgacccc gatttgctgc
     1081 ccgtcatcat ttcagatgcc caaatgcaaa aagccaaagc agaaatgccc gagctgccga
     1141 aagaaatggc agcgcgtttc gtggcggact acggcgtgtc cgaatacgac gcgcgcctgc
     1201 tcaccgcaag ccgcgcacag gccgcctatt ttgaagaagc cgccaaagag agcggacaag
     1261 gcaagccgac tgccaactgg atgaacggcg aactcgccgc cgcactgaac aaagaaggca
     1321 tggaacttgc cgacagcccg attaccgctc cgcgcctcgc cgcgctggta ggcaaaatcg
     1381 ccgacggcac attaagcagc aaat
//
LOCUS       DAWHXZ010000280         1403 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.280, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000280 DAWHXZ010000000
VERSION     DAWHXZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.280"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1110
                     /locus_tag="WIE35_001994"
     CDS             <1..1110
                     /locus_tag="WIE35_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4154864.1"
                     /translation="LSLALTACGGGSDTAAQAPSAKPEAEQSGKLNIYNWSDYVDPET
                     VAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQIKAGAYQKIDK
                     AQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKALGTDKLPENEW
                     DLVFNPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKAAVDMMKAVRG
                     DVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVLTPKTGVGVWV
                     DSFMIPRDAQNVANAHRYIDYTLLPEVAAKNGSFVTYAPASRPARELMDEKYTSDASI
                     FPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            1242..>1403
                     /locus_tag="WIE35_001995"
     CDS             1242..>1403
                     /locus_tag="WIE35_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ4154865.1"
                     /translation="MDLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSKDG
                     SEDICYKYID"
BASE COUNT          397 a          380 c          353 g          273 t
ORIGIN      
        1 ctgagcctcg ccttgactgc gtgcggcggc ggaagcgata ccgccgccca agccccctcc
       61 gccaagcccg aagccgaaca atcgggcaaa ctcaatatct acaactggtc ggattatgtc
      121 gatcccgaaa ccgtcgccgc ctttgaaaaa gaaaccggca tcaagacgcg ttccgattat
      181 tacgacagca acgaaacact ggaggcaaaa gtcctgaccg gcaaatccgg ctacgacctg
      241 accgcgccgt ccatcgccaa cgtcggccgg caaatcaaag cgggcgcgta tcagaaaatc
      301 gacaaggcgc aaatccccca ttacggcaac atcgataaag atttgctgaa aatgatggaa
      361 gccgtcgatc cgggcaacga atacgccgtc ccctatttct ggggcatcaa caccttggca
      421 atcaacacgc ggcaggtgca aaaggcattg ggtacggaca agctgcccga aaacgaatgg
      481 gatttggtgt tcaaccccga atacaccgcc aaactcaaat cctgcggcat cagctatttc
      541 gacagcgcaa tcgaacagat tcccttggcg ttgcactatt tgggcaaaga ccccaacagt
      601 gaaaatcccg aagacatcaa agccgccgtc gatatgatga aagccgtccg aggcgacgtg
      661 aaacgcttca gctcttccgg ctatatcgac gatatggcgg cgggcaacct gtgtgccgcc
      721 atcggttacg gcggcgattt gaacattgcc aaaacccgtg ccgaagaagc cgcaaacggc
      781 gtggaaatca aagtattgac cccgaaaacc ggcgtgggcg tgtgggtgga ttcctttatg
      841 attccgcgcg acgcgcaaaa cgttgccaac gcccaccgct atatcgacta cacgctcctg
      901 cccgaggtgg cggcgaaaaa cggcagcttc gttacctacg cacccgccag ccgccccgcg
      961 cgcgagctga tggatgaaaa atacacctcc gatgcatcga ttttcccgaa caaagaactg
     1021 atggaaaaaa gtttcatcgt atcgcccaaa tccgcagaat ccgccaaact gggcgtgaag
     1081 ctgtggcaag ggctcaaagc gggcaaataa ccggaatccc tgccgtctga aacctttcgg
     1141 gcggcaggaa acggcgcgtc cgttatcaaa cagggggcgt ttccccgccc gcctgttatg
     1201 attgggttaa gattaaaatg atttagtaaa atgagaaaga tatggattta agtatcgtag
     1261 ttcccattta taatgtcgag agttatttgg aagcgtgtct aaattccata gaacctatat
     1321 taagtaatga aaatgtcgaa cttatccttg tgaatgacgg gtcaaaagac ggaagtgaag
     1381 atatatgtta taaatatata gat
//
LOCUS       DAWHXZ010000281         1385 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.281, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000281 DAWHXZ010000000
VERSION     DAWHXZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.281"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..335
                     /locus_tag="WIE35_001996"
     CDS             <1..335
                     /locus_tag="WIE35_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ4154866.1"
                     /translation="ALDGDFACLANLAETIKNHPSVPATAQVLEHMRGSPYEETINRI
                     FRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQKSLQSGLNESEKKLLL
                     SLLTAKQN"
     gene            522..>1385
                     /locus_tag="WIE35_001997"
     CDS             522..>1385
                     /locus_tag="WIE35_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ4154867.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATR"
BASE COUNT          433 a          403 c          309 g          240 t
ORIGIN      
        1 tggcgttgga cggcgatttc gcctgccttg ccaacctcgc cgaaaccatt aaaaaccatc
       61 cttccgtgcc ggcaaccgca caggttttgg aacatatgcg cggttcgccc tacgaagaaa
      121 caatcaaccg catcttccgg tcggcccttc aatcggaaga aatggaaggc ggcggcgaag
      181 aagattgcga aaacttccaa atcggcatca aaaaactgct caatgagtta aaatacagcc
      241 aaatcgaaac attaaaacaa aaaagcctgc aatccggctt aaatgaaagc gagaaaaaac
      301 ttttgctgtc gctgctgacc gcaaaacaaa attgaccggc ggattccgcc atccgtaaac
      361 cgttatgccg tctgaaaagc attcaccccg gctgcaacaa cgacacctgc agaacaccca
      421 tccccaaaag ccttcagacg gcatcagagt accctactct gccacgcctt caggtgcgtc
      481 caaacgcaaa ccgtcggcat cttgccaaca gaaagcagac aatgtccaaa aaccaaaatc
      541 acgaagaata ccaagacgat gcccgccccc tgacgattga ggaacaacgc gcgcgcctgc
      601 gccaactcat cattatgggc aaagaacgcg gctacatcac ctactccgaa atcaatgacg
      661 cactgcccga cgatatgtcc gatgccgatc aaatcgacaa catcgtcagt atgatttccg
      721 gtttgggcat ccaagttacc gaacacgccc ccgatgcgga agacatattg ttaagcgaca
      781 atgccgccgt taccgacgat gatgccgtcg aagaagccga ggccgccctt tccagcgcag
      841 attccgagtt cggcaggacg accgaccccg tccgtatgta tatgcgcgaa atggggcagg
      901 tcgacctgct gacccgcgaa gacgaaatca tcatcgccaa aaaaatcgaa aacgccctga
      961 aaaatatggt tcaggccatc tccgcctgcc cgggttccat tgcggaaatc ttagaactca
     1021 tcgaaaaaat ccgcaaagac gaaatccgcg tcgacgaagt cgtagaagcc attatcgacc
     1081 cgaatgaagt attgctcaac gaattgggct tggggcactt ggaaaccaca gcacccgaga
     1141 aaccttccaa cgacaattcg gacgaaaacg aagacgacga agaatcggaa gaagatgcgg
     1201 atgaaatctc ggcagccaat ctcgccgaat tgaaacaaaa agtcatcggc cactttgccc
     1261 aaatcgaaaa agactacaaa aaaatgattg gccgtttgga aaaacaccac agccggcaca
     1321 aagactatct cgcctaccgc gacgcgattg ccaacaaact gctggaagtc cgtttcgcca
     1381 cccgg
//
LOCUS       DAWHXZ010000282         1376 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.282, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000282 DAWHXZ010000000
VERSION     DAWHXZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.282"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(318..581)
                     /locus_tag="WIE35_001998"
     CDS             complement(318..581)
                     /locus_tag="WIE35_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154868.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            complement(600..>1376)
                     /locus_tag="WIE35_001999"
     CDS             complement(600..>1376)
                     /locus_tag="WIE35_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HEZ4154869.1"
                     /translation="EIVGEALVKNTDFSGMTASEIEKAKANITAYAKLVAGATVGVTG
                     GNVDVAANASETAVKNNALDIIWDIGNLVWDGGKWIYAKSIGDKQMAREAAIDFGVDA
                     AAAAVPFVPAGATKISRGGAYVLKAGDEAVDTAKAIQEIQKQTGIKLTYDKVNKVWTT
                     PAGLDYGLDAKHGNRIKHVLAHTIPNPNKPVHSVFNVSRKEVLPLVDEAWRMKGNPLP
                     NDSSVYLVDMKKPIGTKGETKVRIVVQKGTNKIISAYPQK"
BASE COUNT          378 a          318 c          250 g          430 t
ORIGIN      
        1 taagtgcgcc cgctgccgcc taaaaggcga ccggatgcct gattatcggg tatccgggca
       61 ggattaaggg ggtgtttggg taaaattagg cggtatttgg ggcgaaaaca gccgaaaacc
      121 tgtgttggga tttcggttgt cgtgagggaa aggaattttg caaaggtctc aattggtgtt
      181 ggtgaatgcc ttcggctgcc agcagttgca ttgaggccgt ctgaaaaacg gatattcaga
      241 cggcctattg ctactttaaa taatatttgg gtttaaaata tcatcgtatt tcccaaattc
      301 ggggaacaga aaatcaatta tttaaatttt tcatcctcat aatatgggtg atcaaaaagc
      361 tctccttccc acatatttat ttcttctatg cctttatctt tcataaactc atgataatca
      421 taaaaatctt ttccatattg tccgtaaaaa attttcatat ccccaagcca catagcataa
      481 cattcatcgc ataaaatgac ctccccagag aaatatttag gctttgcatg ataaaggtag
      541 ccttgctcac atcttgggca aattttaatt tctttatcca taagagattc ctttcttaat
      601 tatttctgag gatatgcaga aatgatttta tttgttcctt tttgcacaac aatccgcact
      661 tttgtttctc cttttgttcc aataggtttc ttcatatcta caagatatac ggatgaatca
      721 tttggcaaag gatttccttt cattctccaa gcttcatcaa ccaaaggcaa aacttcttta
      781 cgggacacat taaaaacaga atgaacaggt ttgtttggat ttggaattgt atgggctaaa
      841 acatgtttaa tcctattacc atgcttagca tctaacccat aatctaaccc cgccggtgtt
      901 gtccaaacct tattaacctt atcataagta agcttgattc cggtctgctt ctgaatttcc
      961 tgtatggctt tagccgtatc aactgcttcg tctcccgcct tcagaacata agccccgcct
     1021 cggctgattt tagtcgcacc tgccggaaca aagggaacgg cagctgcggc ggcatccaca
     1081 ccaaaatcaa tcgccgcttc tcgagccatc tgcttatcgc caatagattt ggcgtaaatc
     1141 catttaccgc cgtcccatac gaggttgccg atatcccaaa taatatctaa tgcattattt
     1201 ttaacagctg tttcggaagc atttgccgcc acatcaacat tgcctcctgt aacacctaca
     1261 gtcgctccgg ctaccaattt tgcatacgca gtaatattcg ctttagcttt ttcaatttca
     1321 gaagcagtca taccgctgaa atcggtattc ttaaccaaag cctccccgac aatctc
//
LOCUS       DAWHXZ010000283         1341 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.283, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000283 DAWHXZ010000000
VERSION     DAWHXZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.283"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1140..1340)
                     /locus_tag="WIE35_002000"
     CDS             complement(1140..1340)
                     /locus_tag="WIE35_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154870.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT          449 a          293 c          184 g          415 t
ORIGIN      
        1 cgccgcgctt tccaactctg cactttgatt tttccgtaaa caacaggatt atcgttaaac
       61 atcggtgcag cattcagacg ataagataaa gaaacgacaa cacacgccaa cagaaaaaca
      121 tatttgaact tcatcatatt gtccacataa agggcagcct gaaaatcttt caggctgccc
      181 ttgtcaaatt attcctagct ttcggctttt ttggcaaacc aaacaatccg attacccgca
      241 taatactttc catttattga aatccgacaa gccgcgccca aaaatgccat gcactgtcga
      301 tttccgcagc aatctttgta ccgttttctt caaattccaa atattcaccc aataataaac
      361 ttgaaacaga acgcgtgggc agcaagtgcc caccctacgc ttattcaaat aatttgatta
      421 aataaagaat aaagaaaatg catcaggaac aaaattataa tctgccacct gactcacacc
      481 gcttttaaag taaggggcat caaaatcaaa accgcaaaaa aaataatttt tgcattgatt
      541 tttaatagat ttaaaattcg aatatagtgt ttctccagat ttaaaaagtt tatctttatc
      601 tatatatttg atgctttccc tatccaaaat aaatattcaa acattaaaaa atcattacat
      661 gaccaagcca aatcataaat ttgattcaaa tgggtatcat aaagataaaa ataataaggt
      721 ttgggaacag gtaaaatatt taatggaaaa ggaggactaa ttttcttaat atctgatgac
      781 tgatatccat atctttctat attcttaaaa atttcatctt tcttaagata acaagacatt
      841 ctgaagataa caagacattc tgacaatcac aacctgttca tcggatatat tatctatttg
      901 catggcgcgt ataacacgcc atgcctgatt aaaattagtc tcccttacct tagaatctat
      961 tatcttttcc aaagatgaaa atgcccctta tcttgaactc tccagttaga acctggaagt
     1021 ttcgtacctc ttaaatgtgt attaatattt cttatagttt cattaaaatg ccacgcgctg
     1081 ccgatttcaa cggtaatttt cgtaccgctt tcttcaaatt ccaggtattc ccccattagc
     1141 taacgcaaag aagcagacgc catttcggct tcgttatgat aaacccgcct tccgttgata
     1201 tagacttccg cccctgtccg ctccaaattc caaaaattcc gtactgaaat ttccatatcc
     1261 cgatattgtg cagaccatgt tttttcgaag gttttcataa aatttcctat acctgtccaa
     1321 tcggcacata tcaattgcat t
//
LOCUS       DAWHXZ010000284         1340 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.284, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000284 DAWHXZ010000000
VERSION     DAWHXZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.284"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(205..489)
                     /locus_tag="WIE35_002001"
     CDS             complement(205..489)
                     /locus_tag="WIE35_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154871.1"
                     /translation="MKLALKRSGKYNVYDPLALDLDGDGIETVATKGFSGSLFDHTNN
                     GIRTATGWIAAYDGFSVRKLNSNGGIISTTDTIFQSLHTWLDHQPNDTCH"
     gene            complement(486..779)
                     /locus_tag="WIE35_002002"
     CDS             complement(486..779)
                     /locus_tag="WIE35_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HEZ4154872.1"
                     /translation="MNIDDFTPSKIANFFADPDTYSNVLEEVSRFIYSLVPDDANPWK
                     GGEDYIGRGISEWGELLEKWYKQDFLPYLEKNGTNFRNLKIGCLNSLNGQESG"
     gene            complement(955..1176)
                     /locus_tag="WIE35_002003"
     CDS             complement(955..1176)
                     /locus_tag="WIE35_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HEZ4154873.1"
                     /translation="MTYANFNINGFGDVKLTPYSPLLGYKAWDSFIGSIQSLSDLIYN
                     VDNNRNKMEITVNNAIQAADSFLSSNWKR"
BASE COUNT          457 a          231 c          202 g          450 t
ORIGIN      
        1 gtaataaata aattaaaagc gaaaggtaat taaattgata attgtaaatt atataataaa
       61 caacatacga agaaaaaatt attatagatg atttactata cgaataaaaa aatatcctac
      121 ctagcccgaa ccttcttata atttgtacta tgtgatcatt catttcaata tccatttaag
      181 ctttcaatta attttctata tttttcaatg gcatgtatca tttggttgat gatcaagcca
      241 tgtatgcaaa gattggaata tggtatctgt cgtgctaata atgcccccgt tactgtttaa
      301 tttgcgcaca gaaaaaccgt catatgcagc aatccagccc gtggcggtgc ggatgccgtt
      361 gttggtgtga tcaaataagc tgcctgaaaa gcctttggtg gcaacggttt ctataccgtc
      421 gccgtccaaa tctagggcga ggggatcgta aacgttatat ttgcctgaac gtttgagagc
      481 taatttcaac cactctcttg cccattcagg gaattcaggc agccaatctt caaatttcgg
      541 aaattggtcc cattcttttc aagataaggg agaaaatctt gtttatacca tttttccagt
      601 aactctcccc attcacttat ccctcgtcca atataatctt cgcccccttt ccaagggttt
      661 gcatcatcag gaactaagga atatataaac ctagatactt cttctaatac attgctgtat
      721 gtatcaggat ccgcaaaaaa gtttgctatc ttacttggtg taaaatcatc tatattcatt
      781 atagattcgc caagcctctc gtttccataa tccttattta attcaattca tgctgtaaaa
      841 cagtagagga ttttagcatt tttctcgtat atctcgagat acatttctta aatttaaaaa
      901 aatgttatcg agggatgcaa gtaaagaagc agtatttgtt attttgttat tttgttatct
      961 cttccaatta ctgcttaaaa agctatctgc agcttgaata gcattattaa cagtaatttc
     1021 cattttattt ctattgttat ccacattata gattaaatca gataaggatt gaatagaacc
     1081 aataaatgaa tcccaagctt tatatcccaa gagtggtgaa tagggtgtta atttcacatc
     1141 tccaaaaccg ttaatattaa aatttgcata tgtcatatct gctttccttt caagtaaatt
     1201 aaaaaaataa cgtgaggaat taaaattaaa aataaattaa ttatcgactc aatataatat
     1261 gaatcaatta gctctcttat ggtaaatata agaggtaaaa atactaaaaa taaactaata
     1321 aagttcagaa aaatagtaaa
//
LOCUS       DAWHXZ010000285         1332 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.285, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000285 DAWHXZ010000000
VERSION     DAWHXZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.285"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>1332
                     /locus_tag="WIE35_002004"
     CDS             <1..>1332
                     /locus_tag="WIE35_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ4154874.1"
                     /translation="AANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTN
                     FGTDSKAFQNYYRDLAKRVKNQEIDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQ
                     AFSRTNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYID
                     SQTGEARRGYLDVAKELRERFPDPDEIETEKDKKDFAKLFGEYLRAENVLQNYDEFAA
                     LRELQNVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLS
                     RQKAGEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRR
                     IIRASIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEDL
                     NETAAKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAA"
BASE COUNT          395 a          324 c          352 g          261 t
ORIGIN      
        1 gctgccaacg aagagcagaa cgccgtcggt gaaattgtcg atgaaacctt tgaaccggaa
       61 gcgatggaca gcagcgcgaa agaatttttg caggctgcca tcaacgatta caacgcctgt
      121 ttcaaaacca atttcggcac ggacagcaaa gcctttcaga actactaccg cgatttggca
      181 aaacgggtga aaaatcagga aatagatttg ctgattgtgg tcggcatgtt tttgacgggt
      241 tttgacgcgc cgacgctgaa cacgctgttt gtcgataaaa acctgcgcta tcacggcctg
      301 atgcaggcgt tttctcgcac caaccgcatt tacgatgcca ccaaaacctt cggcaatatt
      361 gtctgcttcc gcgatttgga gcaggcgacc attgatgcga ttaccctgtt cggcgacaaa
      421 aacaccaaaa acgtggtgct ggaaaaaagc tacgaagaat acatgaacgg ctatatcgac
      481 agccagaccg gcgaagcgcg gcgcggctat ttggatgtgg caaaagaatt gcgcgaacgt
      541 ttccccgatc ccgacgaaat cgaaacggaa aaagacaaaa aagattttgc caaactcttc
      601 ggcgaatacc tgcgggcgga aaacgtattg cagaactacg atgaatttgc cgcgctgcgc
      661 gagttgcaga atgtggatgc ggcggacgaa gatgcgatga aggcgtttca ggaaaaatac
      721 tacctgagcg atgaagacgt gcaggaaatg cggcaagtgc cgatgccgtc tgaaagggcg
      781 gtgcaggact accgttccgc ctacaacgac atccgagact ggctgagccg ccaaaaagca
      841 ggcgaacaga aagaacagtc aaaaatcgat tgggacgatg tggtttttga ggtggatttg
      901 ctcaaatcgc aggaaatcaa tttggattac atcttgcaac tggttttcga gcatcacaaa
      961 aaaatcaaag gaaaagcgga gctggtggaa gaaatccgcc gcatcatccg cgccagcatc
     1021 ggccaccgcg ccaaagaggg tctgattgtg gattttatca acgatacgga tttggataaa
     1081 gtccccgacg ttcccgccat actggaaacc ttttacacct acgcgcaaga ggtaatgcgg
     1141 cacgaagcgg caggattgat tgccgccgaa gacctgaacg aaaccgccgc caaacgctat
     1201 ttgaccggct cgctcaaacg cggctatgcc agcgaaaacg gtacggaact gaccgaaacc
     1261 ctgccgaaaa tgagtccgct caacccgcaa tatctgacga agaaacaaag tgtttttcaa
     1321 aagattgcgg cg
//
LOCUS       DAWHXZ010000286         1331 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.286, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000286 DAWHXZ010000000
VERSION     DAWHXZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.286"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..1073)
                     /locus_tag="WIE35_002005"
     CDS             complement(<1..1073)
                     /locus_tag="WIE35_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ4154875.1"
                     /translation="MNKTWKRQVFRHTALYTAILMFSHTGGGGGQAQAQTHKYAIVMN
                     NQKLPEVKWGNDYSSLTDKSNEREVTHTSKFVVAKKHISFSFNNTDEVVAEKKDAVVF
                     GAATYLPPYGKVSGFDTAKLAERKNALDQIGTTHPGLIGYNYQDVTCASGNCPELSYR
                     TQFTFNNGLKRNGNGGRLDIYEDKSRDSSPIYKLKDYPWLGVSFNLGGESSFKPKRQG
                     SLVSSFSEDVTQQNGTQDQYKSKNLVYTTDDYNSKGNKNHQDKHHAVAFYLNAKLHLL
                     DKKQIKNIAQGVTVDLGTLKTRIEPTDAWKNRQGSYFNNGTWTYEEKGSVSVKLKLPE
                     VKAGRCITTPNPNPKSKAPSPALT"
BASE COUNT          294 a          329 c          311 g          397 t
ORIGIN      
        1 gtcagtgccg gcgaaggggc tttggatttg ggattggggt tcggtgtggt gatgcagcgg
       61 cctgctttga cttccggcaa tttgagtttg acgctgactg atcctttctc ttcatacgtc
      121 caagtaccat tattaaaata gctcccctgc ctgtttttcc acgcgtccgt cggctcgatg
      181 cgtgttttca aggtacccaa atcaactgtt acgccttgcg cgatattttt gatttgtttt
      241 ttatccagca aatgcagctt ggcgttcaga taaaaggcga cggcgtggtg tttgtcctga
      301 tggtttttat tacccttact attgtaatcg tctgtcgtat aaacgaggtt tttgcttttg
      361 tattggtctt gtgtaccatt ttgctgcgtc acatcctcgc taaaagaaga taccaaagaa
      421 ccttgtctct ttggtttgaa ggagctctcg ccgcccaaat tgaaagacac gcccaaccaa
      481 ggataatcct tcaatttgta aatgggcgaa ctgtcgcggc ttttgtcttc gtatatatcc
      541 agcctgccgc cgtttccatt tcttttcaac ccgttattga aggtaaattg ggttctatag
      601 ctaagttcag gacagtttcc gctggcgcaa gtgacatctt ggtagttgta gcctatcaac
      661 ccagggtggg tcgtaccaat ctgatcaagg gcatttttgc gctcggccag cttagcggta
      721 tcaaaaccgg aaaccttgcc gtagggcggc agataagtcg ccgcgccgaa aacgacagca
      781 tctttttttt cagcaacaac ttcatcggta ttattgaatg agaaactaat gtgctttttt
      841 gctacaacaa atttactcgt atgggtaact tcgcgttcat tgcttttgtc cgtcaatgag
      901 ctatagtcgt tcccccactt tacctcaggc agcttttgat tgttcattac aatagcgtat
      961 ttatgcgttt gcgcttgcgc ctgccccccc cccccgccgg tatgggaaaa catcaatatg
     1021 gcggtataaa gcgcggtatg gcggaaaacc tgccgtttcc aagttttatt catcttttat
     1081 tccttgagtt tgccttcacg ggacggggcg gcgcgccgcg aacgccgggg ttcggtaaac
     1141 cgcccgattc cgcgcccgcc gaattgctga ttgaaaagct tacttcccca ttttaacttt
     1201 gcacactgat cacggtaaag cgttttttag caaaccgctg cagatgccca acggtctgga
     1261 ttcccgcctg cgcgggaatg acggtttaga agttgcccga aacctcaaaa aaaaccgaaa
     1321 ccgaacaagc c
//
LOCUS       DAWHXZ010000287         1328 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.287, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000287 DAWHXZ010000000
VERSION     DAWHXZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.287"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1107
                     /locus_tag="WIE35_002006"
     CDS             <1..1107
                     /locus_tag="WIE35_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ4154876.1"
                     /translation="TVTVSPSAPYTDTNGLLTDYGNAAASPWMKKIRSVAQGSGETFR
                     ILQIGDSHTAGDFFTDSLRKRLQKTWGDGGIGWVYPANVKGQRMAAVRHNGNWQSLTS
                     RNNSGDFPLGGILAHTGSGGSMTLTASDGKTGKQRISLFAKPLLAEQTLTVNGNTVSA
                     NGGGWQVLDTRAALPLTIQTEMPWDIGFINIENPAGGITVSAMGINGAQLTHWSKWRA
                     DRMNDLAQTGADLVILSYGTNEAFGDNIDIADTEQKWLDTVRQIRDSLPAAGILIIGA
                     PESLKNTLGVCGTRPVRLTEVQQIQRRVARQGQTMFWSWQNAMGGICSMKNWLNQGWA
                     AKDGVHFSAKGYRRAAEMLADSLEELVRAAAIRQ"
BASE COUNT          314 a          458 c          354 g          202 t
ORIGIN      
        1 accgttaccg tttccccgtc cgccccctac accgatacaa acgggctctt gaccgactac
       61 ggcaacgccg ccgcctcgcc ttggatgaaa aaaatccgat ccgtcgcaca aggcagcggc
      121 gagaccttcc gcatcctgca aatcggcgac tcgcataccg ccggcgactt ctttaccgac
      181 agcctgcgca aacgcctgca aaaaacttgg ggcgacggcg gcataggctg ggtttacccc
      241 gccaacgtca aagggcagcg catggcggca gtccggcaca acggcaactg gcaaagcctc
      301 accagcagga acaatagcgg agacttcccg ctcggcggca tcctcgccca caccggcagc
      361 ggcggcagca tgaccctgac cgcatcggac ggcaagaccg gcaaacagcg catttccctg
      421 tttgccaaac ccctgctcgc cgaacaaacc ctgaccgtca acggcaacac cgtctccgcc
      481 aacggcggcg gctggcaggt actggatacc cgcgcggcac tgcccttgac catacagacc
      541 gaaatgccgt gggacatcgg cttcatcaac atcgaaaatc ccgccggcgg cattaccgtt
      601 tccgccatgg gcatcaacgg cgcacaattg acccattggt cgaaatggcg tgccgaccgt
      661 atgaacgacc ttgcccaaac cggcgccgat ttggttatcc tttcctacgg caccaacgaa
      721 gccttcggcg acaacatcga cattgccgac accgaacaaa aatggctgga taccgtccgc
      781 caaatccgcg acagcctgcc tgccgccggc atcctcatca tcggcgcacc cgaatccctg
      841 aaaaacacgc tcggcgtatg cggcacacgc cccgtccgcc tgaccgaagt ccaacagata
      901 cagcggcgcg tcgcccggca gggacaaacc atgttctggt cttggcaaaa cgccatgggc
      961 ggcatatgca gcatgaaaaa ctggctcaac caaggatggg ccgccaaaga cggcgtacac
     1021 ttctccgcca aaggctaccg gcgcgcggcg gaaatgctcg ccgacagcct cgaagaactc
     1081 gtccgcgccg ctgcaatcag gcaataatcg gacaggaggc ggacggtatt tccgcaacag
     1141 ggggatgccg tctgaaacgc ataccttcat attgcttcag acggcatagc caccccgcgc
     1201 acggtttgcc ggacgcaacc ggcattcgcc tcaagcatcg gaaggacggg ggcgaacctc
     1261 cggcatacgg cgcaaaggcg gcgtttgata tgccgtctga aggcaaagat gataaactgc
     1321 cgccttcc
//
LOCUS       DAWHXZ010000288         1320 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.288, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000288 DAWHXZ010000000
VERSION     DAWHXZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.288"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(147..1250)
                     /locus_tag="WIE35_002007"
     CDS             complement(147..1250)
                     /locus_tag="WIE35_002007"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ4154877.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERASILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
BASE COUNT          298 a          365 c          354 g          303 t
ORIGIN      
        1 aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg gtttttgttc atccactata
       61 cctttccgac agccgaacaa aaccccgaat ccgtctgcac ggttcggggt atatctccaa
      121 tacgggcatc gtgttccgga aaaccgtcaa atccgcatcg gcatcacaat atatttgaaa
      181 ttcggattgt tcggcacggt aaacagcgtc gagcggttgg catcgccgaa ggcaagctgc
      241 atatcgtcgg aatggatgtt gcgcaacacg tccatcagat agccgatatt gaaaccgact
      301 tcgagttcgc cgccctgata ggcgatttcg atttcttcgc gcgcttcttc ctgctcgttg
      361 ttgctgcaca caacgctcaa caggccgggt tgcaaaaaca atcgcgcacc gcggaatttt
      421 tcattggcaa gaatcgatgc acgttccaac gcgcccaaca attctgccct cgacaacacg
      481 aaaatcttgt cgttgtccaa aggaatcacg cggttgaaat cggggaattt gccgtcgatg
      541 accttgctga cgatggtcgt gccgttgcat tggaaacgca cctgtttgtc cagcagctcg
      601 atttgaatcg gatcgtcggg gttgttcaac agtttgaaca gttccagcac cgttttgcgc
      661 ggcaaaatca cttcggcgcg cggcaaatcc gcatcaatcg cgcaggctgc ataggcaagg
      721 cggtgtccgt cggtcgccac aaggcgcaac tggctgccct caacctgcat cagcagaccg
      781 ttgagataat agcggatgtc ctgcaccgcc atgctgtact gcacttgcga cagcatggtt
      841 ttgaaacgct cctgctccag cgagaaagtc gcgctgatgt cctcgccgac attcatcatc
      901 ggaaaatcgg cggcaggcag ggtctgcagg gcaaaacgcg atttgcccgc cttcagcgtc
      961 agacggctgt cgtcccaatc cagcgacacc agcgcaccgg caggcagcgc gcgcaaaatg
     1021 tcctgaaatt tcttggcatt ggtggtgatg cggaagtcgc ccgcgccgcc ctcgggaccc
     1081 gcagtgtcga tttggatttc caaatcggtt gccaagagtt tggtctgacc gccttttccc
     1141 tcaatcagga cgttggacag gatgggcagg gtgtggcggc gttcgacaat gccggtaacg
     1201 gcttgcaacg gcttgagcag gctgtcgcgc tcggcttgta aaatcaacat gttcgctcct
     1261 ttaaatcggt ttgtatagtg gattaaattt aaatcaggac aaggcgacga agccgcagac
//
LOCUS       DAWHXZ010000289         1305 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.289, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000289 DAWHXZ010000000
VERSION     DAWHXZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.289"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(383..793)
                     /locus_tag="WIE35_002008"
     CDS             complement(383..793)
                     /locus_tag="WIE35_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154878.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIKGTDFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(797..1264)
                     /locus_tag="WIE35_002009"
     CDS             complement(797..1264)
                     /locus_tag="WIE35_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154879.1"
                     /translation="MQTLDGEMAGGNKPAKQATPNGKAGVSTQQSLQNSLVPQKNYTH
                     TTYNGGSAITDGKYITDPLSMVRHKPDASSLDKIDSKSLFKSDVDAEKATLDAARYAD
                     KHNLWDSQNKAKVPTNDVVGYTDGKPTHIINIYRRKPNKDGFRPVHGTPGKER"
BASE COUNT          381 a          243 c          220 g          461 t
ORIGIN      
        1 ctctacggat ggggcggagt tacccgtatg attgccgtat ggtcggatac ttgtatccga
       61 caaaaatatt tgacgcgtct atcgtttccg aaatgccgct gttgaaaaac gtcggattcg
      121 agaatccgac ctacggttgc tgaacaatgt tttgtagaac ttgctcaaaa tccatgtttc
      181 gccattttcc tttatatata cctaatgcag cacgggttac gatttcgttt aagtcataaa
      241 tagccattta cgtcctctgc taattttcag cgagtagatt gggttaccaa acccaacaaa
      301 aaagcagcct gaaaaatttc aggatgcttg ttttatgttt tttatcaatg aattaggttt
      361 taatccagcc tgcgcttgct agttaaacag tatttacata aaaatcttgc actgaacaaa
      421 caagcgtatc atgttcatca tgaaatgatg ggcataacgt taatctacaa atgtttacat
      481 ttaaagatat tttttcagtt tcaatatttt taatttgtaa ggttaataca tgaggatatt
      541 tgtgatatag ctgatttttt aattgttcgt tgtctaatat agcaaaaggt aattttgcac
      601 acaaataacc aataactttg tttttatgaa aaaaaactat ccaattttca attccttcat
      661 cccaatcaaa agtaaaatcc gttcctttta tgataaattt agcacaagat attaaatcta
      721 acttaaccca agtggattta tacagactca atgctgatga agttataata ttatctaaat
      781 tataaatttt cataaatcaa cgttccttac ctggtgttcc atgcacaggt ctaaacccat
      841 ctttattcgg ctttctacga taaatgttaa ttatatgtgt cggttttccg tctgtatagc
      901 caactacgtc gttcgttgga acttttgcct tattttgact atcccataaa ttatgtttat
      961 ctgcatatct agcagcatct aatgtagctt tttccgcatc aacatctgat ttaaataaac
     1021 ttttgctatc tatcttgtct agactagatg catcaggttt gtgtcgtacc atactgagag
     1081 ggtcagtaat atatttacca tctgtaatag ctgatccacc attataggta gtatgcgtat
     1141 aatttttttg tggaacgaga ctattttgca aagattgttg tgtagaaacg ccagctttgc
     1201 cattaggagt agcttgcttc gctggcttat tcccaccggc catttcccca tctaaagtct
     1261 gcataggtcg ggcagcaggt gttttatagc cggtattttt aaccc
//
LOCUS       DAWHXZ010000290         1290 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.290, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000290 DAWHXZ010000000
VERSION     DAWHXZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.290"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            166..1278
                     /gene="nadA"
                     /locus_tag="WIE35_002010"
     CDS             166..1278
                     /gene="nadA"
                     /locus_tag="WIE35_002010"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ4154880.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEASTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPEAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSHPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGHNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
BASE COUNT          308 a          343 c          377 g          262 t
ORIGIN      
        1 accaaagcgg tttgtcggaa cgaagcgggt cggttttacg gtatcgcaaa cctgttttca
       61 gacgaccttg cttattatac tcaatttgag cataatataa aaatatttca aaatataaac
      121 ggcaaatctt tatgccgtct gaaaaaccag ccataaggaa gtctcatgca aaccgccgcc
      181 cgccgctcgt tcgattacga tatgcccctc atccaaaccc cgacttccgc ctgccaaatc
      241 cgtcaggcgt gggcgaaggt tgccgacacg cccgaccgcg agacggcagg ccgtctgaaa
      301 gacgaaatca aggctttgct gaaggagaaa aacgcggtct tggtggcgca ttattacgtc
      361 gatccgctga ttcaggattt ggctttagaa acgggcggat gcgtgggcga ttcgctggaa
      421 atggcgcgct tcggcgcgga acacgaagct agtacattgg tggtggcggg tgtgcgcttc
      481 atgggcgaga gcgcgaaaat cctctgccct gaaaaaacag tgctgatgcc tgatttggag
      541 gcggaatgtt ctttggattt gggctgtccg gaagaagcgt tttcagcgtt ttgcgaccaa
      601 caccccgacc gcacggtcgt agtgtacgcc aacacttccg ccgccgtgaa agcgcgtgcc
      661 gattgggtgg taacgtcttc ggtggcgttg gaaatcgtat cgtatttgaa atcgcgcggc
      721 gagaagctga tttggggacc tgaccgccac ctcggcgact acatccgccg cgaaacgggc
      781 gcggatatgc tgctgtggca gggttcgtgc atcgtccaca acgaattcaa agggcaggag
      841 ctggcggcgt tgaaggcgga acaccccgaa gcggtggtgc tggttcatcc cgaatcgcca
      901 caaagcgtca tcgaactggg cgacgtggtc ggctcgacca gcaaactgct caaagccgcc
      961 gtatcgcacc ctgaaaaaaa attcatcgta gcgaccgact tgggcatcct gcacgaaatg
     1021 caaaagcagg cacccgacaa agaatttatc gccgcaccga cggcgggcaa cggtggaagc
     1081 tgtaaaagct gcgcgttctg cccttggatg gcgatgaatt cgctgggcgg catcaaatac
     1141 gccctgacaa gcggacacaa cgaaatcctg ttggacagga agctgggcga agccgccaaa
     1201 ctgcctttgc agcgtatgct cgacttcgcg gcaggactca agcgcgggga tgtgttcaac
     1261 ggcatgggac ccgcctgatt tgccgtccat
//
LOCUS       DAWHXZ010000291         1281 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.291, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000291 DAWHXZ010000000
VERSION     DAWHXZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.291"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(199..1011)
                     /locus_tag="WIE35_002011"
     CDS             complement(199..1011)
                     /locus_tag="WIE35_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ4154881.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGEVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVLDAV"
     gene            1096..>1281
                     /locus_tag="WIE35_002012"
     CDS             1096..>1281
                     /locus_tag="WIE35_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEZ4154882.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADS"
BASE COUNT          259 a          409 c          351 g          262 t
ORIGIN      
        1 ggaaatgact gaaacccccg agattctaga ttcccacttc cgtgggaatg acggttcagt
       61 tgcgtaggac tggggcgtcg aaaggggcgg attcgatgga ttcgatgaaa acggtagaaa
      121 tgtgggattg atggaaatgg caggctgaag cccatcctac agcccgctga ttccccggca
      181 ccgatgccgt ctgaaccttc agacggcatc caaaacccgg tatttcaagg cgggcaggcg
      241 gttgcggacg ctgttcaggc ggttggcatc gatgtctgcc gtaacaacgc cttcgccttc
      301 gggcaatacg tccaacacgt cgccccacgg atcgacaatc atgctgtgtc cgaacgtgcg
      361 ccgtccgttt tcgtgcaaac cgccctgtgc cgccgccacg acgtaacatt ggttttcgac
      421 ggcacgcgcg cgcagcagca gctcccaatg cgccttgccc gtcgtgtgcg taaacgcagc
      481 gggcagcatc aatacgtcaa acggcagctg gcggcggaaa aattcgggaa agcggacatc
      541 gtaacaaata cctgccgcca ccggcacgcc ttctgccgac aagtgcggca cttccccgcc
      601 cgcgcggatg gtatcggctt cggcatagcg ttcgcccaaa ccggaaaagc cgaagaggtg
      661 cattttgtgg tacagccccg tccgtttgcc gtcgcgcccg tacaccaata aagtattcat
      721 gaccttgccc gcctcggggc tttgcagcgg cacggttccg ccaaacagca ccacgccgca
      781 ttctttcgcc gtttcgctca atgccgtctg aaagcgtccg ccgcccaaag gctcggcaag
      841 cgcgagtttg tcggtatcgt ttgcgcccat cagcacccaa tattcgggca gcagcaccca
      901 atccgcaccc tgctccgccg cccgtgcgac caggcgtttc atggcggcga cgttggtttc
      961 cggcgacacg cccgacacca tctgcacggc ggcaactctg attttgtcca tttcttctcc
     1021 tttttcctgc cgaccttgcg gcatattccg ccgataggct accatgacgg tactttgcga
     1081 aaaccgttta cacaaatgcc cattttgaac catattgccc tcatcggtgt agggctgatc
     1141 ggcggttcgt tcgttctcga cctcaaaagg cagggactcg tccgcaccgt taccggtatc
     1201 gacaccgacc gcgacaacct cgaacgtgca ttggaacgcg gcgtgattga ccgggcttcc
     1261 gttgccatcg acgcggacag c
//
LOCUS       DAWHXZ010000292         1253 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.292, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000292 DAWHXZ010000000
VERSION     DAWHXZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.292"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            236..1189
                     /locus_tag="WIE35_002013"
     CDS             236..1189
                     /locus_tag="WIE35_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ4154883.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPASFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAAQYTVMPLLAIGIAKLFDLSPGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFPGNPESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
BASE COUNT          200 a          272 c          432 g          349 t
ORIGIN      
        1 aaaccggatg attcgggcta aataattcct gattctttgg atatagccaa cggtatgcct
       61 gccgatgccg tcagaaaggg cttgatgggc gtttgcggtt cgggttaata tttgtgctgt
      121 atctgttaat tccggttagt aaaatggggc ggggacgatg agtggccgtc ggtcggtttt
      181 gcacgggcgg taataatgtc cggcgcaagg tgcgccgttt cgggaggggt gtgtgatggg
      241 ttttctgatt ggtttgagcc gcaacctgac gcggtttacg gctttggtca ttttgcttgc
      301 gtcggcggtt gcgtttatcg gaccggcttc gtttgcgtgg gttaagggcg atgttcaggt
      361 cttggtgctg ggcatcatta tgctgggtat ggggatgact ttgggcaggg aggactaccg
      421 gattttggcg cagcgtcctt tggatgtgtt tatcggtgcg gcggcgcaat ataccgtgat
      481 gccgttgctc gccatcggta ttgccaagct gttcgacctt tcgccggggc tgactttggg
      541 gctggtgttg gtggggacgt gtccgggcgg cgtgtcgtcc aacattatga gtttccttgc
      601 caaaggcgat gtggcgtttt cagtcggtat gacgacggtg tctacggtgc tggctccggc
      661 cgttacgcct ttgtggatga cctgcctggt cggtcagacg gtggatatgg acggttgggg
      721 gatgttccgg tttatgctgc tggtcacgct tttgccggtg gtgctgggtt cggcggcaaa
      781 tatcctgctg cacggaaaac attggtttga aaatgtccgt gccgttatgc cgggcgtggc
      841 ggtattggca tttgcctgca ttgtcggcgg cgtggcttcg gttcacggac accgttttgc
      901 cgaatcggcg ttggtaatgg tgttggcgat tgcggtacac aatatcgccg gctatgtgtc
      961 aggctattac gccgccgcgc tgtttggtat ggatacggca aaaaaacgca ccttggcaat
     1021 cgaagtcggc gtgcagaatg ccggtttggc aacaggattg agcgcgaaat ttttcccagg
     1081 caaccccgaa tcggcggttg cggcggcggt cgcctgcgtg tggcattcgg tttcggggac
     1141 ggtattgggc aacttgttcg ctctgtggga caagcgtcgg gacgcttagt cgggcgtgtc
     1201 cgaaccgtcc cacgatatat agtggattaa ctttaaaccg gtacggcgtt gcc
//
LOCUS       DAWHXZ010000293         1205 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.293, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000293 DAWHXZ010000000
VERSION     DAWHXZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.293"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(308..1162)
                     /locus_tag="WIE35_002014"
     CDS             complement(308..1162)
                     /locus_tag="WIE35_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ4154884.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          326 a          353 c          312 g          214 t
ORIGIN      
        1 agtgaatcgg ttccgtacta tctgtactgt ctgcggcttc gtcgtcttgt cctgattttt
       61 gttaatccgc tataaaatcc cgtcattcct gcgcaggcgg gaatccggac atccaacgct
      121 gcagcaattt gtcggaaata accgaaactc aaaaaaccgg atgcccgcct gcgcgggaat
      181 gacgatgcaa agatggtcgt tatttcggat aaatatccgt cttttcgttc cggatacccg
      241 ctttcgcagg aatggagatg gaaagatttc agcctattcg gcaatcatca aataccgctt
      301 cccaacttta tttttgatgc gacagccggc cggcaaccaa ggtggcggcg atgacgacaa
      361 acacgcccaa ggcggacacg ggcgacaggt gttcgcccaa aatcaaaacc gccagcagca
      421 cgccgacgac gggttcgagc gaaatcaaca gtcccgaaac gttggcagga acacggctca
      481 tccccttgtt ccacagccaa taggcgtacc agccgcaccc caaacccaaa tacagcagcg
      541 acaataccat cccgacgctc cagtccacgg tataactttg cgccaaagca agcgaaaacg
      601 gcaggcacat caacgatgcg gcggcaatgg aaacagatgt gaatgccggt gcgccgatgc
      661 gtgcaatcag cctttgcgtc ggacgcatag cggcacaaaa gcccgcgccc gccaacaaca
      721 ccagcaggca gccgaaccag ccgacttcgc cgccctcttc cgcaccgccc gccatcagca
      781 gcgcgacacc ggcaaatgcc gccgcgccgc atatccagtg gtaggcacgc gctttgtcgt
      841 tgaagaaaaa gtgtccgaca aacaccatca gcagtggctc gagtccgaca atgaccgatg
      901 cgctggcggc ggaagtgtat ttcaacccga caaactgaag cagcagggtc agcacatagt
      961 tgacgaacga cacaatcagc aacggcttcc attcctcacg cggaatcttg ccgacatgac
     1021 ggcggcaggc gggcagtgca ggcagcgcgg caattagcag gcgcacgccg accatcaatg
     1081 cgggatcgat gccgccatag acatatttgg cggcaataaa cgagctgctc cagataatca
     1141 gggcaaggat ttggtaaaac atagtgagtg ataaagtcag atggtcatac aaaagtatag
     1201 cggat
//
LOCUS       DAWHXZ010000294         1192 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.294, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000294 DAWHXZ010000000
VERSION     DAWHXZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.294"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>1192
                     /locus_tag="WIE35_002015"
     CDS             <1..>1192
                     /locus_tag="WIE35_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ4154885.1"
                     /translation="VSDSLKAAAVSTESSLPPGKSNLHGQIQQQPAPSPVGEGWGEGK
                     AVAAQTEFPASATNPLPSPLPQEREQSAAAVSDDLKAKSSLHLVASNLHGQIRQQPTP
                     SPVGEGWGEGKAVSAQTEFSAAAISPLPSPLPQEREQSAAAVSDDLKTKSSLHPVASN
                     LHGQIRQQPTPSPVGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVS
                     DGLLRDLAEINIGDPVVHEEHGIGRYMGLITMDLGGETNEMMLLEYAGEAQLYVPVSQ
                     LHLISRYSGQAHENVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYARRAAQSGHKF
                     EINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRA
                     AFVAV"
BASE COUNT          291 a          376 c          323 g          202 t
ORIGIN      
        1 cgtttcagac agtctgaaag cagccgccgt ttcaaccgaa agcagcctgc cccccggtaa
       61 aagtaatctg cacgggcaaa tccaacagca acctgccccc tcccccgtgg gggagggttg
      121 gggagagggc aaagcagttg ccgctcaaac cgaatttccc gcatccgcaa caaaccctct
      181 ccctagccct ctcccacagg agagggaaca aagtgcagcc gccgtttcag acgacctgaa
      241 agccaaaagc agcctgcatc tcgtcgcaag taatctgcac gggcaaatcc gacagcaacc
      301 tactccctcc cccgtggggg agggttgggg agagggcaaa gcagtttccg ctcaaactga
      361 attttccgca gccgcaataa gccctctccc tagccctctc ccgcaggaga gggaacaaag
      421 tgccgccgcc gtttcagacg acctgaaaac caaaagcagc ctgcatcccg tcgcaagtaa
      481 tctgcacggg caaatccgac agcaacctac tccctccccc gtgggggagg gttggggaga
      541 gggcaaagca gttgccgctc aaagtgccat cgccgtcatc accgaatccg atctctacca
      601 atacgtcgcc cgttcgcgcg tccacaaccg ccgcaagaaa cacgccgccg tttcagacgg
      661 tctgttgcgc gaccttgccg aaatcaacat cggcgacccc gtcgttcacg aagaacacgg
      721 catcgggcgg tatatgggct tgatcacaat ggatttgggc ggcgaaacca acgaaatgat
      781 gttgcttgaa tatgcaggcg aagcgcagct ttatgtgcct gtttcgcaac tgcatttaat
      841 cagccgctac tccggtcagg cgcatgaaaa cgtcgccctg cacaagctcg gcagcggcgc
      901 gtggaacaaa gcgaaacgca aagccgccga aaaagcgcgc gataccgccg ccgagttgct
      961 taacctttac gcccgacgcg ctgcccaatc gggacacaag tttgaaatca acgagttgga
     1021 ttatcaggcg tttgccgacg gcttcggcta cgaagaaacc gaagaccagg cagccgccat
     1081 cgccgccgtg attaaagatt tgacgcaggc gaagccgatg gaccgcctcg tgtgcggcga
     1141 tgtcggtttc ggcaaaaccg aagtcgccct gcgcgccgcg tttgtggcgg ta
//
LOCUS       DAWHXZ010000295         1155 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.295, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000295 DAWHXZ010000000
VERSION     DAWHXZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.295"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..66)
                     /locus_tag="WIE35_002016"
     CDS             complement(<1..66)
                     /locus_tag="WIE35_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ4154886.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAV"
     gene            complement(202..726)
                     /gene="nspA"
                     /locus_tag="WIE35_002017"
     CDS             complement(202..726)
                     /gene="nspA"
                     /locus_tag="WIE35_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ4154887.1"
                     /translation="MKKALAALIALALPAAALAEGASSFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGMRVKF"
     gene            complement(896..1090)
                     /locus_tag="WIE35_002018"
     CDS             complement(896..1090)
                     /locus_tag="WIE35_002018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154888.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKPYACTCNNPTDTA
                     VFSKPFASFTHPPRDAAATI"
BASE COUNT          232 a          256 c          381 g          286 t
ORIGIN      
        1 gacggcttgg ctgtacgcgc cgatggcggc gggggctttg tcggtgtgga tgatggtttt
       61 ggacatttcg gattcctcaa aaaatagggc ggcagaagcc gcagcattcg ggattatcgt
      121 acaaaaccgc cggcttgtgt agttgcggtg gcagaaaaca aaaccgccga aggctcggcg
      181 gtttgcagaa taaggcgcat atcagaattt gacgcgcata ccggcggaca gttcgccgga
      241 acggacgttt ttgacagtgt tgactttgcc gatgtagttg tagcggtagc cggcatccaa
      301 atcgacattc ggggtaacgg catagcttac gcccgccaat acgccgaggc cggtggaggt
      361 ttggctgaag ctgtcgctgc cgcccaagtc gacggaggcg cggttgaggc tcaagcgcgc
      421 gccgagatac ggtttgacgg gcgattgggt gtcgaagtcg taaatggcgg acgcgccgat
      481 gctgtaaagt ttgaaatcgg tggatggggc tttatagttt ttgtagcgcg tgtaatcgac
      541 ggcgaagcgg aggtcgttga tgcggtagcc tgcggagatg cgcgggctga agcctttggc
      601 agaacctaaa gagcttgagg cttttgcgtg tgcggcatcg gcttggacgt aaaagctgga
      661 tgcgccttcc gccagtgcgg cggccgggag agcgagggca atcagtgcgg caagtgcttt
      721 tttcatattt tggttccttt atggtcagtt agaaaaattg ttaagaatcc gttaaagaat
      781 cctgctgtat tatacttaaa ttttcttttg catcgtaata ttttcaatac ttcaagatac
      841 gtagcggtat ccggctgctt tgccgacggc aaagccgtta acccgcgcgt tgcctttaaa
      901 tggtggcggc ggcatcacgc ggcggatggg tgaaacttgc aaacggtttg gaaaaaacag
      961 cggtatctgt cggattgttg caggtgcagg catacggttt tgtgtgcgtc tgtgccttaa
     1021 gcgtcggaca tttccggcgg cggctgtgcc gtctgaaacg cccggcgggg gatgcggctg
     1081 cgttttccat cgataagcat attttccgga cgcgttcggg gcgggttttc ccgggcggcc
     1141 gccgatttgt ttgcg
//
LOCUS       DAWHXZ010000296         1146 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.296, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000296 DAWHXZ010000000
VERSION     DAWHXZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.296"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(129..>1146)
                     /gene="rpoD"
                     /locus_tag="WIE35_002019"
     CDS             complement(129..>1146)
                     /gene="rpoD"
                     /locus_tag="WIE35_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ4154889.1"
                     /translation="RKLEREIRDICLDRVHMERDYFIQNFLPEITNLEWIEEEIAKGR
                     VWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSSEKETAAAKQEMIQA
                     NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
                     RSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLAELMQMPEDKIRKIM
                     KIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHEVTKEILESLTPREA
                     KVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRHPTRSDRLRSFLDSE
                     DSKL"
BASE COUNT          204 a          249 c          331 g          362 t
ORIGIN      
        1 cttggtggga atttagggga ttttggggaa ttttgcaaag gtctcgggat aggagtgtgg
       61 gatatgtgga agcgttgttt attgtgtaag aaaaaaccgc aggtatttga aacctgcggt
      121 tttttggttt acagcttgct gtcttcgctg tccaagaaac ttctcaaacg gtcgcttctt
      181 gtcggatgcc gcagcttgcg gagtgctttt gcctcgattt gtcggatgcg ttcgcgcgtt
      241 acgtcaaact gtctgccgac ttcttccagc gtgtggtcgg tgttcatatc gatgccgaaa
      301 cgcatacgca ggacttttgc ctcacgcggg gtcaggcttt cgaggatttc tttggttact
      361 tcgtgcaggc tggtgtacat tgccgcatcg gccggcgcaa cattgttggc atcttcgatg
      421 aagtcgccca agtgcgaatc gtcgtcgtcg ccgatggggg tttccatcga aatcggctct
      481 ttggcgattt tcatgatttt gcggattttg tcttcgggca tctgcatcag ttcggcaagt
      541 ttggcggaat cgggttcttc gccggtttct tgaaggtgtt ggcgcgagat gcggttcatc
      601 ttgttgatgg tttcaatcat atgtaccgga atgcggatgg tacgcgcctg atcggcaatc
      661 gagcgtgtaa ttgcctggcg gatccaccag gttgcgtagg tggagaattt atagcctctg
      721 cggtattcga acttatcgac cgctttcatc aaaccgatgt tgccttcctg aatcagatca
      781 aggaattgta agccccggtt ggtgtatttt ttggcgatgg aaatcacgag gcgcaagttt
      841 gcctgaatca tttcctgttt ggcggctgcg gtttcttttt cgctcgacac catatttttg
      901 ttgatttctt tcaactcttc gatggaaatg cgggtttctt tttccatatc cgccaactcg
      961 gtttgttttt cgaggatggc gtggcggaag cggtcgagcg cgtcgctcca aaccctgcct
     1021 ttggcgattt cttcttcaat ccattccaga ttggtgattt cgggcaggaa gttttggatg
     1081 aagtagtcgc gctccatatg gacgcggtcg aggcagatgt cgcggatttc gcgttcgagt
     1141 ttgcgg
//
LOCUS       DAWHXZ010000297         1144 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.297, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000297 DAWHXZ010000000
VERSION     DAWHXZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.297"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            120..848
                     /gene="rpe"
                     /locus_tag="WIE35_002020"
     CDS             120..848
                     /gene="rpe"
                     /locus_tag="WIE35_002020"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ4154890.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPYTLEKIRQV
                     RAMLDRYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(871..>1144)
                     /locus_tag="WIE35_002021"
     CDS             complement(871..>1144)
                     /locus_tag="WIE35_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ4154891.1"
                     /translation="GIADAALSMLDVDAQGLDVMDRKFLEAVLHKFGGGPVGLDNVAA
                     AIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAYLHFGLPVEK"
BASE COUNT          262 a          282 c          325 g          275 t
ORIGIN      
        1 tctctaaggt gctgaagcac caagtgaatc ggttccgtac tatctgtact gtctgcggct
       61 tcgtcgcctt gtcctgattt ttgttaatcc actataaaag gatattgaag gaaaaaaaca
      121 tgactactta ccgtatcgca cccagcattt tatcggcaga ttttgcacgg cttggcgaag
      181 aggtggaaag cgtcattgcg gcaggtgcag atctgatcca ttttgacgtg atggacaacc
      241 attatgtgcc gaacctgacc ttcgggccta tggtttgcgc ggcgttgaag ccttatgcaa
      301 gcgtgccgat cgatgtgcat ctgatggtcg aacccgtgga tgacctgatt cagtcgtttg
      361 ccaaagcagg agcatcaatc atcacgttcc atcccgaagc gagccgccat attgaccgca
      421 gcttgagcct gattcgggac atgggctgtc aggcggggct ggtgttgaat ccggcaacgc
      481 ccgtatatct gttggaaaac gtattggaca ggctggatat ggttttgctg atgtcggtaa
      541 accccgggtt cggcggtcag agcttcattc cgtatacttt ggaaaaaatc agacaggttc
      601 gtgccatgct tgaccgctat gaagagaaaa gcggcagacg tattgccatt gaagtggacg
      661 gcggcatcaa aaccgacaat atcgccgccg ccgcccaagc cggcgcggat acctttgttg
      721 ccggttctgc gattttcggc aaaccagact ataaggcggt gattgatgcg atgagggcgg
      781 aactggaaaa aagcggcagg ctgaaccttg cgccaatgat tgaaatagaa atgccgtctg
      841 aaaattagct ctgtttcaga cggcattgcg ttatttttcg acgggcagcc cgaaatgcag
      901 gtaggcgcgt tcggtcgcca tcctgccgcg cggggtgcgt tgcaggaagc cttgttggat
      961 aaggtagggt tcgataacgt cttcgatggt gtctgtagat tcgccgatgg cggcggcaac
     1021 attgtccaaa ccgaccgggc cgccgccgaa tttgtgcaaa acggcttcga gaaatttcct
     1081 gtccatcacg tccagcccct gcgcgtccac gtccagcata cttaaagcgg catcggcgat
     1141 gccg
//
LOCUS       DAWHXZ010000298         1097 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.298, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000298 DAWHXZ010000000
VERSION     DAWHXZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.298"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..734)
                     /locus_tag="WIE35_002022"
     CDS             complement(<1..734)
                     /locus_tag="WIE35_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ4154892.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMAYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAA"
     gene            complement(811..1002)
                     /locus_tag="WIE35_002023"
     CDS             complement(811..1002)
                     /locus_tag="WIE35_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154893.1"
                     /translation="MFQQRTAHQAPHCVLPERDYPPIRFYRYKQMGFNRKGMGIKSIS
                     DTSRAMPSENQSPLSDGIV"
BASE COUNT          299 a          269 c          292 g          237 t
ORIGIN      
        1 ttggcggcaa agaagccgag caggtagccg atgccgttgt ggagtacgac aaccgcaaaa
       61 atcagcaggc cgctttccat aatcttgcct ttgcttgccc caacaaccgc gccgataatc
      121 agcacgatgg cggcaacgga aaccagcggc agcgcatcgg tcagcttttc ggttttactg
      181 cccaaaacct tatggacaat caaacccaaa acaatgggga gcaaaaccat tttgacgatg
      241 gacatcaaca taccggccgc ttggatttcc agcatttcac cggcaagcat caggaagatg
      301 gcgggagtca gcaatgggga aatcagggtg gaaacagacg taacggcaac cgacaaagcc
      361 acattgccgc gcgccagata ggccatcaca ttggaagccg taccgcccgg gcagcagccg
      421 accaaaatca cgccgaccgc gatttcggca ggcaggttca acagtttgga cagcagccag
      481 gcggttgccg gcataatggc gaattgtgcg attacgccga tgatgacggc tttgggatgt
      541 ttgaacaaaa tatcgaagtc ggaaggtttg agcgtcaaac ccataccgaa cataataatg
      601 cccaacagcc aaggaatata aggccccgcc catttgaagg tgtcgggcgc gaaaaaagcg
      661 gcggcggcaa agagcgcggc ccagagggaa aatgtttttc cgataaagct gctgatttta
      721 ctgaggatat tcataataat gcgttgcgtg tttgttttta agggaaggca agcatacacg
      781 ccttaacctt aatttgcaaa atgaccgtgc ctaaacaatg ccgtctgaaa gtggagattg
      841 gttttcagac ggcatcgccc gagaggtgtc ggaaatggac tttatcccca ttccttttcg
      901 gttgaaaccc atctgtttat agcgatagaa tctaatcgga gggtagtctc gttcgggcaa
      961 cacgcagtgc ggtgcttgat gtgccgtcct ctgttgaaac atataaagct cggagaaagt
     1021 atagtggatt aaatttaaac cagtacggcg ttgcctcgcc ttgccgtact atttgtactg
     1081 tctgcggctt cgtcgcc
//
LOCUS       DAWHXZ010000299         1073 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.299, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000299 DAWHXZ010000000
VERSION     DAWHXZ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.299"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            43..507
                     /locus_tag="WIE35_002024"
     CDS             43..507
                     /locus_tag="WIE35_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ4154894.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT          358 a          214 c          214 g          287 t
ORIGIN      
        1 actctatttg ttaaaatcaa taataaatac aaggtaatcc aaatgaaaga actagataaa
       61 atcgatttcc gcattcttaa gatcctccaa cagaatgccc gcatcccgat gacggagctt
      121 gccgagaagg taggcttgtc caccacgccc gttacagaga gggtgcgccg tttggagcgg
      181 gaacattata tttccggcta tcacgcccat ctcaatcccc atctgttggg caaaccacta
      241 ttggtttttg tcgagctgaa gctgcaatcc aaatcgggca atattttcga agatttcaaa
      301 aaagaagtgc tgaaaattcc gcaaattatg gaatgccact tggtgtcggg cgaatacgac
      361 tatctgatta aagtacgttt gcccgatatg tctgcctatc gggatatgct aggcaatatc
      421 ctattgcaac tgcctgccgc ttcggagagt aggagttatg ttgtgatgga ggaggtcaaa
      481 gaaaatccgg ttttggattt ggattgaata tttcaggctg gctatgcggt ttgacaccta
      541 aatgaaacat aacaacatca cgcacagagg cttgttgctt cagataaaaa aatgcacaca
      601 ttcaatagaa tgtgtgccaa gtctacaaag gagaaataga ggagaaacta ttaactgact
      661 actcgaacca gctaacggaa cgaattatgc agcttttccg gtcaaaaagc aagcgttttt
      721 cttgttaaat ttcttaaaga atggaatttc aaacaataaa agtaattaaa tttcaaaaaa
      781 agcggagata aaaaaatgca cacatccggt tgaatgtgtg ccaagtctac aaaggagaaa
      841 tagaggagaa aaatcaagct gcatcaagca actcgacggc gggaattatc cgccgtttct
      901 tatccgctgt caattatttt ttgaaaattt acccgaccga ttcaaatttt gcaaaaaact
      961 tgcccgatgg ctatccggta acaaattcct tcaaaaaccc atcaatccgc ttattcaaaa
     1021 aaatcatatg atttcattga aataaacttt tgattcgcta aatttgctga aat
//
LOCUS       DAWHXZ010000300         1058 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.300, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000300 DAWHXZ010000000
VERSION     DAWHXZ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.300"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            168..791
                     /gene="lolA"
                     /locus_tag="WIE35_002025"
     CDS             168..791
                     /gene="lolA"
                     /locus_tag="WIE35_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ4154895.1"
                     /translation="MMKPHNLFKFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
BASE COUNT          266 a          344 c          254 g          194 t
ORIGIN      
        1 ggcacacatt aaagggcagc gtggcgcgcc tgccttttcc gagcggtcaa aaaaatcagc
       61 cctcggaaaa cgcggtttgt aaaatgcaaa ccgcccgtaa cgccgcccgt atgattgttt
      121 tgccgcgccg atactttacg ccacacccat cccaacaagg aaaaataatg atgaaaccgc
      181 acaacctgtt caaattcctc gccgtttgct ccctgaccgt ctccgtcgct tccgcacagg
      241 cgggcgcggt ggacgcgctc aagcaattca acaacgatgc cgacggtatc agcggcagct
      301 tcacccaaac cgtccaaagc aaaaagaaaa cccaaaccgc gcacggcacg tttaaaatcc
      361 tgcgcccggg cctcttcaaa tgggaataca cttcgcccta caaacagact attgtcggcg
      421 acggtcaaac cgtttggctc tacgatgtcg atttggcaca agtgaccaag tcgtcccaag
      481 accaggccat aggcggcagc cccgccgcca tcctgtcgaa caaaaccgcc ctcgaaagca
      541 gctacacgct gaaagaggac ggttcgtcca acggcatcga ttatgtgctg gcaacgccca
      601 aacgcaacaa cgccggctac caatacatcc gcatcggctt caaaggcggc aacctcgccg
      661 ccatgcagct taaagacagc ttcggcaacc aaacctccat cagtttcggc ggtttgaata
      721 ccaatcccca actctcgcgc ggcgcgttca agtttacccc gcccaaaggc gtggacgtgt
      781 tgagcaactg atgccgtccg ccccgatgcc gtctgaaagc cgccgaggct tcagacggca
      841 ttttcacgca ggcggaacaa tgtcccgcat taccgcccga tcgggcatcg gaacggcaaa
      901 cccgtgaaaa ttaacggttg cgccccggtt gtttttgccg tttaatgcaa accttgctgc
      961 accaagggcc aagaaagccg accggccgcc cccacagctt ccgatgcagg cggccaggcc
     1021 gtccctgcaa tgttttttat ttttgaacga aaggtcga
//
LOCUS       DAWHXZ010000301         1048 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.301, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000301 DAWHXZ010000000
VERSION     DAWHXZ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.301"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..893
                     /locus_tag="WIE35_002026"
     CDS             <1..893
                     /locus_tag="WIE35_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ4154896.1"
                     /translation="ATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAK
                     ANLPKDAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAQNPALKNLS
                     PAQRRELADNIIAEKVRQQAVMQNSRVGEAEVDRFIEQAQKQGITLPEGAPLRQYRAQ
                     HILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGV
                     MVPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKA
                     EQATVNLLRDLHSGAYVDIR"
BASE COUNT          275 a          334 c          277 g          162 t
ORIGIN      
        1 ccgccacggc gcaaaaagaa gccgcacccg cacaacagca gggcggtatc cgcttttcag
       61 acggcattgc cgccgttgcc gacaacgaag tcatcacgcg ccgccggctt gccgaagccg
      121 ttgccgaagc caaagccaac ctgcccaaag acgcgcagat aagcgaatcc gagctgtccc
      181 gacaggtgct gatgcagctt gtcaaccaat ccctgatcgt acaggcgggc aaacgccgca
      241 acattcaggc aagcgaagcg gaaatcgatg ccgtcgtcgc acaaaatccc gccctcaaaa
      301 acctcagccc cgcccaacgc cgcgagcttg ccgacaacat cattgccgaa aaagtccgcc
      361 agcaggcagt gatgcaaaac agccgggtcg gcgaggcaga ggtcgaccgc tttatcgagc
      421 aggcgcaaaa acaaggcatc accctgcccg aaggcgcacc gttgcgccaa taccgcgccc
      481 aacacatcct gattaaggcc gacagcgaaa acgccgccgt cggcgcggaa agcaccatcc
      541 gcaaaatcta cggagaggcc cgcagcggca cagacttttc cagcctagcg cgccaatatt
      601 cgcaagacgc gagcgcgggc aacggcggag atttgggctg gtttgccgac ggcgtgatgg
      661 ttcccgcctt tgaagaagcc gtccacgcgc tcaaacccgg acaggtcggc gcgcccgtcc
      721 gcacccaatt cggctggcat atcatcaaat tgaacgaagt gcgcgatgcc ggcacacctc
      781 aggaacgtat ccgcaattcc gtgcggcaat acatcttcca acaaaaagcc gaacaggcaa
      841 ccgtcaacct gttgcgtgac ctgcattccg gcgcgtatgt cgacatccgc taaggcggtt
      901 tgaagcaaaa agccataccg atcggcaaaa atccgggcgg tatggctttt tggatttcga
      961 gttactttta caccgtcatt catcattccc gcgtaggcgg gaatctagca acgaaaagta
     1021 acaggaattt atcgggaaaa cagaaacc
//
LOCUS       DAWHXZ010000302         1037 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.302, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000302 DAWHXZ010000000
VERSION     DAWHXZ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.302"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(21..212)
                     /locus_tag="WIE35_002027"
     CDS             complement(21..212)
                     /locus_tag="WIE35_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154897.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            341..901
                     /locus_tag="WIE35_002028"
     CDS             341..901
                     /locus_tag="WIE35_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ4154898.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINFPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          298 a          286 c          208 g          245 t
ORIGIN      
        1 caacgacgta ccggtttttg ttaatccgct ataccacgat gaatccttcg caatatctgt
       61 ttatcgacct caattttgac aaaataccgg atacgcgcct ttgttgcttt tccatcttcc
      121 aaccaactgt aaatctcaaa cagccggtac acgccatgct tcagtttctt ttcctgtcgg
      181 cggattgttt cgacaaagaa ttgaaaatcc atttcatgca ccttaaaatt taatctgcat
      241 tcaaaccttt tcactttgga agcaccattt atcggatgtc ccttcgcaat aaacaaattt
      301 tcccgatacc gccgcccatt tcaacccaaa cccaaaagct atgaaaaacc tcatcgcctt
      361 caacaaaccc tatggcgtta tctgccaatt ttcaccgcac gaaaaacaca aaagcctcaa
      421 agactttatc aattttcccg gcttctaccc cgccggacgg ctcgacaccg acagcgaggg
      481 gctgctgctg ctgaccgacg acggcaggct tcaggcacaa attaccgacc ccaaattcaa
      541 acaccctaaa acctactggg cgcaactgga gggcgtaccc gacgaaagcc gattggaaag
      601 cctaagaaaa gggatagact taggcggttt cgttacccgt ccggcaagca tccgcatctt
      661 gaaacacgga gaagcagatt cgttatggga gcgcatcccg ccgatacgcg tccgcaaaac
      721 cgttcccgat ttttggattg aaattaccat ttctgagggc aaaaaccgcc aagtcaggcg
      781 aatgaccgcc aaggcgggct atccctgcct gcgtctgatc agagtggcaa gcggcagact
      841 gaaactgttt gatttggatt taaaacccgg ggaatgggca tacgccccgt ttaaaccata
      901 atcacgttta tctcatcatt tccacaaaag tgggaatccg gaattttata gtggattaac
      961 aaaaatcagg acaaggcgac gaagccgcag acagtacaaa tagtacgaaa ccgattcact
     1021 cggtgcttca gcacctt
//
LOCUS       DAWHXZ010000303         1034 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.303, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000303 DAWHXZ010000000
VERSION     DAWHXZ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.303"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(128..877)
                     /locus_tag="WIE35_002029"
     CDS             complement(128..877)
                     /locus_tag="WIE35_002029"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ4154899.1"
                     /translation="MPSVELLLEIVGLPRGTFYYQLAVQSAEDKYADLKRHIHDIYRR
                     HKGRYGYRRIAAAIRHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNI
                     LRRCFHAEKPNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEI
                     LKGAPEKPGSSEKPILHSDQGWQYQMFFYSGLNLNQDKAMKPQTVQIVRQGEATPYWF
                     KFNPLYNNDRIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(882..>1034)
                     /locus_tag="WIE35_002030"
     CDS             complement(882..>1034)
                     /locus_tag="WIE35_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ4154900.1"
                     /translation="QTKKAGYLKTKEELLAELACLTAEMAALKKPDALIHGKEVRQKE
                     RNSSQG"
BASE COUNT          243 a          266 c          217 g          308 t
ORIGIN      
        1 taaaaaggtt acccaaaact taaatgtaat cctttattta taagcttgca gctttccaaa
       61 caagttaccc ccaaacggat aaaaaaatgc cgaagccgaa ctgaccccaa aaagtcggac
      121 agtttaatca agcggctttc agggactgaa ttctgtactg aacaaggctc agccctttta
      181 atttcaactt gattctatcg ttgttgtata gtggattaaa tttaaaccag tacggcgttg
      241 cctcgccttg ccgtactatt tgtactgtct gcggcttcat cgccttgtcc tgatttaaat
      301 ttaatccact ataaaaaaac atctgatatt gccaaccttg atccgaatgc agtatcggct
      361 tttcagacga tcccggtttc tccggcgcac ctttcagtat ctcgccggcc aaatcgaaag
      421 tcgggcggat ttgaatacgg taactgacga tttccccgtt aaacaaatcc ataatcggag
      481 aaaggtatat cttttctccg cctacattga actcggtaac gtccgttacc catttctcat
      541 tcggcttttc tgcatggaaa cagcgtcgca ggatattcgg cgcaattttg ccgacttctc
      601 ctttgaacga gcggtatttg cgccgccgta tcactgcctt cagccccgtc ttcgccatca
      661 gacggctgac tttcttgtga ttgaccggtg ttcctgcgtg acggatggct gccgcaatcc
      721 tccggtagcc gtatcttccc ttatgtcgtc gataaatatc atggatatgc cgtttcaaat
      781 cggcatattt gtcttctgcc gattggacgg ccaattggta atagaaggtg ccgcgtggca
      841 gtccgacaat ctccaacagc agttcaacgg atggcattgc cttaaccctg cgacgagttg
      901 cgttctttct gccgcacttc tttcccatgg attaaggcat cgggcttttt tagggcagcc
      961 atttccgctg taaggcaagc caattccgca agcagttctt ccttggtttt cagatagccg
     1021 gcttttttcg tttg
//
LOCUS       DAWHXZ010000304         1029 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.304, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000304 DAWHXZ010000000
VERSION     DAWHXZ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.304"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(88..720)
                     /gene="mtrR"
                     /locus_tag="WIE35_002031"
     CDS             complement(88..720)
                     /gene="mtrR"
                     /locus_tag="WIE35_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ4154901.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          245 a          228 c          265 g          291 t
ORIGIN      
        1 atgtattttt agaagaaggc tttgtgtcat tttgatgccg tctgaaaggg tttgttcgtt
       61 tcagacggca ttgctaccaa ggcttgatta tttccggcgc aggtcgggat ggttttccaa
      121 gttgtccatc attatcccga tgatgcgcgg ggcggttttg cccaaatcga aacgttcgca
      181 ggaagagaac caccgccaaa tcagcccgtc caaggttgat ttgatgaaga taactgccgt
      241 ttccttgtcc aaatcgtcag ccaaatcctg attttccacc gcttcggtca aaacggcggt
      301 aattttctcg cgccagattg cctgatgctt gcgggcaatg gcgataacgg cggcgttttg
      361 ctccgtgtgt tcgcatttta aaaacaggat gttgtggaat ttgtagtaga tgtcgttgct
      421 ttgcagccgc tcgaaaaagt gcagcagcgt gtggcggaat accgcccaag accctccttc
      481 ggcatcttcg gcatcttgcg cgatgcagtt ttcgatgtcg tcgcagatac gttggaacag
      541 cgcgtcgaac aagtcttcct tatttttgaa atgccaatag agcgcgccgc gcgttacgcc
      601 ggcggcttgg gcgatttcgt tgagcgaggt gcgcgcaatc cctttgcggt aaaaggtttc
      661 caaggcggca agcatcaggt gttctttggt tttcaaggct tcggttttgg tttttctcat
      721 aatggcgttt tcgtttcggg tcggtttgat gagggcggat tataaaaaag actttgtaac
      781 catgcaatcg tgtatgtata atgaaaccca tgaaattgag actacatctc aactttgaaa
      841 acaggcataa cctgctttgc aacccgttcg aacattcggg tatcaaagca ggcggttttt
      901 atatcctgaa atatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt
      961 acaaatagta cggaaccgat tcacttagtg cttcagcacc ttagagaatc gttctctttg
     1021 agctaaggc
//
LOCUS       DAWHXZ010000305         1007 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.305, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000305 DAWHXZ010000000
VERSION     DAWHXZ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.305"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..823
                     /locus_tag="WIE35_002032"
     CDS             <1..823
                     /locus_tag="WIE35_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ4154902.1"
                     /translation="EFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMS
                     VSVRYDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQNKQNQPPTLVPAVVGKPGSDVY
                     YAGLNYKNGGFAGNYAFKYAKHANVGRDAFELFLLGSGSDEAKGTDPLKNHQVHRLTG
                     GYEEGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERG
                     KKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          249 a          263 c          245 g          250 t
ORIGIN      
        1 cgaattcggc acgctgcgcg ccggtcgcgt tgcgaatcag tttgacgatg ccagccaagc
       61 cattgatcct tgggacagca acaatgatgt ggcttcgcaa ttgggtattt tcaaacgcca
      121 cgacgatatg tcggtttccg tacgctacga ttcccccgaa ttttccggtt ttagcggcag
      181 cgtccaattc gttccggccc aaaacagcaa gtccgcctat acgccggctc attttgttca
      241 gaataagcaa aatcagccgc ctactctcgt tccggctgtt gtcggcaagc cggggtcgga
      301 tgtgtattat gccggtctga attacaaaaa tggcggtttt gccgggaact atgcctttaa
      361 atacgcgaaa cacgccaatg tgggccgtga tgcttttgag ttgttcttgc tcggcagcgg
      421 gagtgatgaa gccaaaggta ccgatccctt gaaaaaccat caggtacacc gcctgacggg
      481 cggctatgag gaaggcggct tgaatctcgc cttggcggct cagttggatt tgtctgaaaa
      541 tgccgacaaa accaaaaaca gtacgaccga aattgccgcc actgcttcct accgcttcgg
      601 taatgcagtt ccacgcatca gctatgccca tggtttcgac tttatcgaac gcggtaaaaa
      661 aggcgaaaat accagctacg atcaaatcat cgccggcgtt gattatgatt tttccaaacg
      721 cacttccgcc atcgtgtctg gcgcttggct gaaacgcaat accggcatcg gcaactacac
      781 tcaaattaat gccgcctccg tcggtttgcg ccacaaattc taaatatcgg ggcggtgaag
      841 cggatagctt tgtttttgac ggctcgcctt cattctttga ttgcaatctg actgccaatc
      901 tgcttcagcc ccaaacaaaa atccggatac ggaagaaaaa cggcaataaa gacagcaaat
      961 accgtctgaa agattttcag acggtatttc gcattttcgg cttggtt
//
LOCUS       DAWHXZ010000306          978 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.306, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000306 DAWHXZ010000000
VERSION     DAWHXZ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.306"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            9..>978
                     /locus_tag="WIE35_002033"
     CDS             9..>978
                     /locus_tag="WIE35_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEZ4154903.1"
                     /translation="MLEKQHFPRFVIGESLLPHCMEMLEEAGFADAVRAEPGFQLKNG
                     AAFSWGSRYTEFDFTDKFSDGPGTTYQVRRAVFDKILIEEAAKQGVEVRFGHGVTAFD
                     NSGDFVRLNIETDTGKRYELTAKFVLDASGYGRVLPRLLNLETPSHLPPRQAHFTHID
                     DNITNPKFDRNKILITTYPQHRDVWIWLIPFGDNRCSVGVVGTPDKLAGESETVLKKF
                     VYECPMLNEILDKAVWENDFPFRSIQGYSANVKSLHGRHFALLGNAAEFLDPVFSSGV
                     TIALHSAKLAADLLAKQLKGEAADWQTEFAEPLMIGVDAFRTYSGLN"
BASE COUNT          230 a          290 c          253 g          205 t
ORIGIN      
        1 aagtctgcgt gttggaaaaa cagcactttc cgcgcttcgt catcggcgaa agcctgctgc
       61 cgcactgtat ggaaatgctg gaagaagccg gatttgccga tgccgtgcgc gccgagcccg
      121 gctttcagtt gaaaaacggc gcggcgtttt cgtggggcag ccgttatacc gaattcgatt
      181 tcaccgataa attttcagac ggccccggca cgacttatca agtgcgccgc gccgtgttcg
      241 ataaaatcct gattgaagaa gccgccaaac aaggcgttga agtacgtttc gggcacggcg
      301 taaccgcgtt cgacaacagc ggcgattttg tccgcctgaa catcgaaacc gacaccggca
      361 agcgctatga actgaccgcg aaattcgtct tggacgcaag cggttacgga cgcgtgctgc
      421 cgcgcctgct aaacttggaa acgccctcgc acctgccgcc gcgccaagcg catttcacgc
      481 acatcgacga caacatcacc aacccgaaat tcgaccgcaa caaaatcctg attaccacct
      541 atccgcaaca ccgcgacgtg tggatttggc tgattccctt cggcgacaac cgctgttccg
      601 tcggcgtggt cggcacgccc gacaaacttg ccggcgaatc ggaaacagtg ttgaaaaaat
      661 ttgtttacga atgcccgatg ctgaacgaaa ttttggacaa agccgtttgg gaaaacgatt
      721 ttccgttccg ctccatccaa ggctattccg ccaatgtgaa atcgctgcac ggcaggcatt
      781 tcgcgctatt gggcaatgcc gccgagttcc tcgatcccgt gttctcgtcg ggcgtaacca
      841 tcgcgctgca ctccgccaaa cttgccgccg atctgttggc aaaacaactc aaaggcgaag
      901 ccgccgattg gcaaaccgaa tttgccgaac ccttgatgat cggcgtggac gcgttccgca
      961 cctatagtgg attaaatt
//
LOCUS       DAWHXZ010000307          975 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.307, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000307 DAWHXZ010000000
VERSION     DAWHXZ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.307"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            43..>975
                     /locus_tag="WIE35_002034"
     CDS             43..>975
                     /locus_tag="WIE35_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ4154904.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKKLVLAARSLA
                     EAHYPADAVTPYGEPLSDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LTFDGLFDIRAVRILVDTIPECYTTLGIVHSLWQP"
BASE COUNT          247 a          305 c          235 g          188 t
ORIGIN      
        1 ctgttacaat aaactcgttt ttatcggaac gaaagaccca tcatgaccgc catcagcccg
       61 attcaagaca cgcaaagcgc gactctgcaa gaattgcgcg aatggttcga cagctactgc
      121 gccgcgctgc cggacaacga taaaaaactt gtcttagccg cccgttcgct ggcggaagca
      181 cattaccccg ccgatgccgt cacgccgtat ggcgagccgt tgtccgacca cttcctcggc
      241 gcggcacaaa tggtcgacga actcgacctg ctgcccgatg ccgtcgccgc caccctgctt
      301 gccgacatcg gacgctacgt ccccgattgg aatctattgg tttccgaacg ctgcaacagc
      361 accgtcgccg agctggtcaa aggtgtggac gaagtgcaga aactcaccca cttcgcccgg
      421 gtggacagcc tcgccacgcc ggaagaacgc gcccagcaag cggaaaccat gcggaaaatg
      481 ctgctggcga tggttaccga catccgcgtc gtgttaatca aactggcgat gcgtacgcgc
      541 accctgcaat ttttaagcaa cgcccccgac agccccgaaa aacgcgccgt tgccaaagaa
      601 accctcgaca tcttcgcccc gctcgccaac cgtttgggcg tgtggcagct caaatggcag
      661 ctcgaagatt tgggcttccg ccatcaagaa cccgaaaaat accgcgaaat tgccctgctt
      721 ttggacgaaa aacgcaccga acgcctcgaa tacatcgaaa acttcctcaa tatcctgcgt
      781 acggaactga aaaaatacaa tattcatttt gaagttgccg gaagaccgaa gcacatctac
      841 tccatttaca aaaaaatggt gaagaaaaag ctcactttcg acggcttgtt cgacatccgc
      901 gccgtacgga ttttggtgga caccattccc gagtgttata ccacgctggg cattgtccac
      961 agcctctggc agccg
//
LOCUS       DAWHXZ010000308          957 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.308, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000308 DAWHXZ010000000
VERSION     DAWHXZ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.308"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..561)
                     /locus_tag="WIE35_002035"
     CDS             complement(<1..561)
                     /locus_tag="WIE35_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154905.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRLVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGL"
BASE COUNT          271 a          312 c          188 g          186 t
ORIGIN      
        1 cagcccctcc gtttttttcc gaaaacgccc cacattttcc aaaaccggtg ccggcaaatc
       61 caattttgcc gcctccgcaa aatgcctttc tgccgacttc agtcggaaat cgtcaaactc
      121 cgccgccgcc aaatccagca aaatccgctc gtctgccgca ttttccccgt gcagttcccg
      181 ataccgcgcc accgcctccg ccggccttcc cgccaatttc gccagcaaag cccgcgccct
      241 gccgtacaaa accgcgtcat aatccggcag cttggcatac aaatccgcca acgaagcgat
      301 taaatccgcc tgattgccgt tgagcgcgtc gcgcaaacta tgttccaaca ttttcggatg
      361 cgccaacaaa aaatccccgt caaccacgcg cggggcatca ttttcaactt tccaatccga
      421 ttccgcccac ttatccgaca ccaaccgttg cacctgcaac aatgccttgt catccaaaat
      481 cgcgggcgca tccgccccat aggcggcaga aacacctgcc gcacaccaaa caaccaaaaa
      541 gccgtatctg aaatacaaca taccctgtca tttacctttc tggcaaacac gccgccgaag
      601 cacgtcaaac catccgaaaa acaggcagaa acccgtgaaa accggctttg ccgtctgaaa
      661 gcgggcaggc aaaaaaccgc cgcccgattt tcaaagggcg gatttcacat ttatagcgga
      721 ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acaaatagta cgaaaccgat
      781 tcactcggtg cttcagcacc ttagagaatt gttctctttg agctaaggcg aggcaacgcc
      841 gtaccggttt ttgttaatcc actataacag caaccctgtc gccgtcattc ccacgaaagt
      901 gggaatctag aaatgaaaag caacaagcat ttatcggaaa taactgaaac cgaacag
//
LOCUS       DAWHXZ010000309          955 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.309, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000309 DAWHXZ010000000
VERSION     DAWHXZ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.309"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>955
                     /locus_tag="WIE35_002036"
     CDS             <1..>955
                     /locus_tag="WIE35_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ4154906.1"
                     /translation="RGGGRAQAETRNYAIVMNKQNQPEVKREGSYSTLREKDRKRKFD
                     FNANNGGGGSVSFNNSDELVSRQNGTAVFGTATYLPPYGKVSGFDDKRLKERGNAVNW
                     IHTTHPGLIGYSYAGVVCRNSTGCPQLVYKTRFSFDNTGLAKNAGMLDSHPDPSRENS
                     PIYKLKDYPWLGVSFNLGSENTVKNSQSSNRLISSFSENNNNQTIVSTTESHPISLGD
                     GQREHTAVVYYLNAKLHLLNKKQIQNITDKTVQLGVLKPSIDVKTQRTGFGGILSFWA
                     SWDIKDTGQIPVKLGLQQVKAGRCVNANNPNKSTKAPSPALT"
BASE COUNT          279 a          241 c          232 g          203 t
ORIGIN      
        1 ggcggggggg ggggcgcgcg caggcggaaa cccgtaacta cgctattgtc atgaacaaac
       61 aaaatcagcc ggaggtaaag cgggagggtt catattcaac cttaagggaa aaagacagga
      121 aacgcaaatt tgactttaat gcgaacaacg ggggtggagg ctctgtctca ttcaacaata
      181 gcgatgagct tgtttctcga caaaacggta ctgccgtttt tggcacagcc acctacctgc
      241 cgccctacgg caaggtttcc ggttttgatg acaaaaggct gaaagagcgc ggcaatgccg
      301 ttaattggat tcatacgacc cacccagggt tgataggcta cagctacgcc ggtgtcgtat
      361 gcagaaacag cacaggctgt ccccaacttg tctataaaac ccgattttcc ttcgacaaca
      421 ccggtttggc aaaaaatgcg ggcatgctgg atagtcaccc ggacccaagc cgcgaaaatt
      481 cgcccattta caaattgaaa gattatccat ggttgggcgt atctttcaat ttgggcagcg
      541 agaataccgt caaaaatagc caatcatcca acagattgat atcttctttt agtgaaaaca
      601 ataataatca aaccatcgtc tctacgacag aaagccaccc tatttccctt ggcgacgggc
      661 agcgcgaaca taccgccgtg gtctattatc tgaacgccaa gctgcacctg ctgaataaaa
      721 aacagattca aaatatcacc gacaaaacag tgcagttggg tgtcttgaag ccgagcatcg
      781 atgtgaagac acaaaggacg gggtttggcg gcattctatc tttttgggct agttgggaca
      841 ttaaagatac cgggcagatt ccagtcaagc tcggcctgca gcaagtcaaa gcaggccgct
      901 gcgtcaacgc aaataacccc aataagagta ccaaagcccc ttcccccgcc ctgac
//
LOCUS       DAWHXZ010000310          951 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.310, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000310 DAWHXZ010000000
VERSION     DAWHXZ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.310"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            111..842
                     /locus_tag="WIE35_002037"
     CDS             111..842
                     /locus_tag="WIE35_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ4154907.1"
                     /translation="MSREINAGRHHFLYGGISGGGVHYWDNKDFSEQSLRLSFGYKNR
                     SVTRSFGIVPFVEQNLLGGSRYNFVGGFNADFSQRLSERWRLTLNAGNMWKHYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHNITKEAEQASIRKGLRVGAVKTFDG
                     GLGLRANLRYTRRTFDAPGTIVYRFPRKDHEYQANLSLWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNMQIFMSVEKDFK"
BASE COUNT          247 a          257 c          254 g          193 t
ORIGIN      
        1 ctataaacaa cgcttcgtcc aaaaaaacga ttgaatttgc gggcagaagc tggacgaaaa
       61 ccgccgacag cctgccgcaa aaggcacacg gtttgcgcta gggcttaggc gtgtcgcgcg
      121 aaatcaatgc gggcaggcat catttcctct acggcggcat cagcggcggc ggcgtgcatt
      181 attgggataa caaagatttc agcgaacaga gcctgcgcct gtcgttcggc tataaaaacc
      241 gttcggtaac gcgctcgttc ggcatcgtgc cgtttgtcga gcaaaacctc ttaggcggca
      301 gccgatacaa tttcgtcggc ggcttcaatg ccgatttctc ccaacgcttg agcgaacgct
      361 ggcggttgac actaaacgcg ggcaatatgt ggaagcatta tcaggaagac cgcaccgccg
      421 cccgatacga cagccatatg ccgctggcgg gcgcgacgct gatgtattcc gcgccgaaag
      481 actggctgct ttacggcggt gcggactggt cgcacaacat aacaaaagag gcggaacagg
      541 cttccatccg caagggtttg cgtgtcggcg cggtcaaaac gttcgacggc gggctgggtc
      601 tgcgggcaaa cctgcgctat acccgcagga cgtttgatgc acccgggacc attgtgtacc
      661 gcttcccgcg taaagaccac gaatatcagg caaacctgtc gttgtggcac gacaaaatct
      721 cttggaaggg atttacgccg caactcaatt tccgctatct gaaaatcgac agcaatatga
      781 aaagttttta cacacgcaaa aacatgcaga ttttcatgag cgtggaaaag gatttcaaat
      841 aagcgcaaaa aatgccgtcg gcaacatccg tgagcagaat caaaaacagc cgcatcattt
      901 attgtcaacg cctgcgccgt cagagtaaca ttgcgttttt cccccaccga t
//
LOCUS       DAWHXZ010000311          949 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.311, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000311 DAWHXZ010000000
VERSION     DAWHXZ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.311"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..549)
                     /gene="earP"
                     /locus_tag="WIE35_002038"
     CDS             complement(<1..549)
                     /gene="earP"
                     /locus_tag="WIE35_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ4154908.1"
                     /translation="MNTYAFPVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWT
                     DDVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLH
                     IIRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCE
                     AVRFDSEALRQRLMLPEKNAPEW"
     gene            complement(568..846)
                     /locus_tag="WIE35_002039"
     CDS             complement(568..846)
                     /locus_tag="WIE35_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154909.1"
                     /translation="MFRNLLVRWLAVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKHQTMFLFIPIVLLVVYLFHYFGAF"
BASE COUNT          277 a          242 c          242 g          188 t
ORIGIN      
        1 ccattcgggg gcgttttttt cgggaagcat cagccgctga cgcagggctt cgctatcgaa
       61 acggacggct tcgcagtaat cgcgttcgcg tatcagtccg ccgctttttt cgctgaaacc
      121 cataaaccaa aaatattttt gaacaccttc ctgcggcgaa ggcatcaggt gcagcctttc
      181 attgctttcc tccgcgctca aatattccca attcagccaa agcggcttgt gtcggcggat
      241 aatgtgcagc acattttcgg gcaggtcgca ggcaaaagtt tcgatgacgg catcgggaac
      301 aggcgcggta tcaatatctg ccgcatcgga atgccaagtg cggacatgaa tatcctgatg
      361 aacgcaggga acatcgggca aatcagggca aagcgcgcgc aaggcggaca catcgtccgt
      421 ccacaaatgc acctgccaac cgagttcgcg gtgcaaaaca cgggcaagcc gccacgaaac
      481 gccgatgtcg ccgaaattgt cgatgacctt gcaaaaaatc caacagacag gaaaagcgta
      541 tgtattcata accatctaga aaacaagcta aaatgcgcca aaataatgga acaaatacac
      601 aaccagcaaa acaatcggaa taaacagaaa catagtttga tgtttcaaat caaactcttg
      661 tttcaaatga tgcttgatgg tgtcgaaaag gacgaatttg aacagaaacg tcgcttcgca
      721 ggcaaggatg atgccgttaa tcagatgctg gggtttgtct tcgggcggat tggcggcgaa
      781 aacggcaagc gtcgccaagg gtatcaggca gacggcaagc cagcggacaa gcaggtttcg
      841 gaacatcgca aggctttcaa acaaaaaaag caaaacgcca ttatcgcccc aagcacgcgc
      901 cttgtctaac ggatgcgccg acgatgccgt ctgaagcctt gccgcagga
//
LOCUS       DAWHXZ010000312          939 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.312, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000312 DAWHXZ010000000
VERSION     DAWHXZ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.312"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            670..921
                     /locus_tag="WIE35_002040"
     CDS             670..921
                     /locus_tag="WIE35_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin B"
                     /protein_id="HEZ4154910.1"
                     /translation="MYIPLPTVIAIQSNIMAIVSTPHPALSALIILAHYHGIAAKSLG
                     LKAKVVRQPVKRLAMVTLPALVWCDDGNHFKEKWLFSAF"
BASE COUNT          269 a          204 c          151 g          315 t
ORIGIN      
        1 gtatataaaa tatatttttt ggcatctaaa aaataaactt ttagcaatca tatatgaata
       61 gcttgtagaa tagaaaacga aaattattaa ttatcgtagg tgtgcattta ttccaacacg
      121 gtttcatgca gcttaaaaac cagaaattct ttctcccttt atcctttctt aaaaatagcg
      181 tttccaaatt ctgcaacgac aggtttcatt caagtaggac tgccgtgatt gcatcaattg
      241 cttcgcggca tggcatccat atcacacaac aggacattta ttctcgggta agtccacctt
      301 catcctcttc ggccagcagg aacagtaaaa tatgctctgt actcggatag taaatatttc
      361 ctttctcact attgtttgtc atttaatcga cagtctactc tttctctaaa atttcgtcaa
      421 taatagttaa atatatttaa atacatttaa aataataata tacacctgtt tcagtctgtc
      481 taaaacacgg gaaaatatgt tatttgatca caacggtgac tttgggcagc ctgtaccttc
      541 aataacgcgt ggaaaataga tatatactta tatcgtattc gaggctgtct tgtttccctc
      601 aaacaaccat tcttccacac ttttgcttta caattttcgt gtgtatcctt acaattaccc
      661 ctattgtttt tgtatattcc tttgccaacc gttattgcta tccaatctaa tattatggct
      721 atcgtatcta caccacaccc cgctctttcc gccctcatca tcctcgccca ttatcacggc
      781 attgccgcca aatctttggg attgaaggca aaggtagtac gccagcctgt caaacgtttg
      841 gctatggtga ccttacctgc attggtatgg tgtgatgacg gcaaccattt taaggaaaag
      901 tggttgtttt cggcattctg aaacgcaacg gacgggtct
//
LOCUS       DAWHXZ010000313          937 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.313, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000313 DAWHXZ010000000
VERSION     DAWHXZ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.313"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..848)
                     /gene="mafB"
                     /locus_tag="WIE35_002041"
     CDS             complement(<1..848)
                     /gene="mafB"
                     /locus_tag="WIE35_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ4154911.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGDGRGSVKNRVYAVQTFDATAVGPILPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WGNRMDDIRGIIQGAANPFLTGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAA"
BASE COUNT          218 a          233 c          213 g          273 t
ORIGIN      
        1 gccgcaagtt gtgcttccgg acttaaattt cctaaattgt gaataccctg taaagttttc
       61 cgtgctgccg cataggttac cgggtttacc gcactgtctg taattgcccc aactcccaat
      121 ccctgaaaac ccgttagaaa aggattggct gcaccttgga tgatgccgcg aatatcatcc
      181 atacggttac cccaccaatt tttattgggg tcgctttccc atatcagttt tcctgcttca
      241 tccgcacggc tgaaaaaacc agaggcggca ccggcatttt cttttagcca acggtctgaa
      301 aatggggctt gagggacaat attagtcttt gtttttgttg aagttccttt gacatagtag
      361 ctgtatatat cccttgctcc tccggggggc ggataatcgc tgccttgcgg tccgtcatat
      421 ccatcctccg gatggatttc cgaccctgtc cgatgaagtt ggtaaacagt aaaaccgccg
      481 tctacaccgc cgctgaaatc agaagtgctt tttgaatcat gatgatcgaa cggactgtgt
      541 acttcatgtc cgtgccctga aaaatgggtt tcatagccga taacaccttc aaaccctgtc
      601 cgttcgtgtg taataggcag tatggggccg accgcagttg catcaaatgt ttggacggcg
      661 taaacccgat ttttaacact gccgcgacca tcaccgaaca gatggtattt gccacccggt
      721 tcgtagtgtt ttgcctgcat atcatcgcgc aaacgggcat ccaaaccgtt cgcatgacta
      781 atggggattt gcagcaacga tattgctgct gcaaacagca tcatgaattt ttgaataggc
      841 aaattcattt tgttttcctt ggtagcaagc ggttatggta gtgtgaatca aggttgccct
      901 tgtctatgtt gtcgcactac ttcatcgctg tatccat
//
LOCUS       DAWHXZ010000314          924 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.314, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000314 DAWHXZ010000000
VERSION     DAWHXZ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.314"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..236
                     /locus_tag="WIE35_002042"
     CDS             <1..236
                     /locus_tag="WIE35_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein-releasing system transmembrane
                     subunit LolC"
                     /protein_id="HEZ4154912.1"
                     /translation="GVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDIGDVA
                     LIACISLGLSFVATLYPSRRASKTQPAEALRYE"
     gene            229..924
                     /gene="lolD"
                     /locus_tag="WIE35_002043"
     CDS             229..924
                     /gene="lolD"
                     /locus_tag="WIE35_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ4154913.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVLVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLSQRQLGILRNHHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
BASE COUNT          194 a          242 c          288 g          200 t
ORIGIN      
        1 gcggcgtgct tttgggttgg aacgtcggca gggtcgtggc gttttttgaa aacctgctcg
       61 gtgtccacct catcaattcg caggtttatt ttatcgacta cctgcccagc gatgtcgata
      121 taggcgacgt tgccctgatt gcctgcattt ccttggggct gtctttcgtg gcgacgctct
      181 acccgagccg gcgcgcgtca aaaacccaac cggcggaggc tttgcgttat gagtgagttg
      241 attttgaaat gcgaaggcgt gggcaaacgc tatcgggacg gcggtttgga cgttctggtg
      301 ctgcacggct tggatttgga aatccgcgcg ggggaaagca ccggcatcat cggttcttcg
      361 ggcagcggca aatcgacgct gctgcatatt ttgggcgggc tggatatgcc gtctgaaggc
      421 agggtgctgc tgatgggcga ggatttacgt acattaagcc agcgacaact gggcatcctg
      481 cgcaaccacc atctcggttt cgtgtaccag ttccaccatc ttctgcctga attttcggca
      541 ctggaaaacg taatgatgcc gcttctgatc ggcaaaaaaa gccgtgaaga ggctgcggag
      601 gcggcaatgg cgatgctgga aaaggtcgga ctgaaacacc gttcgacgca ccgcgcgggc
      661 gaactttcag gcggtgagcg gcagcgtgcc gccattgcac gcgccttggt tacccaaccc
      721 aaatgcctgc ttgccgacga accgaccggc aatctcgatc gtgcgaacgc caggaatgtt
      781 ttggatatga tgctggaact gaaaacagaa ttgggaacgg ggctggtggt cgtaacgcac
      841 gacgacgaac tcgccggccg cttcgagcgc gtgatggtca tgaaagacgg cagcctgcac
      901 cccaaacagg gcgcaaacgc ctaa
//
LOCUS       DAWHXZ010000315          911 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.315, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000315 DAWHXZ010000000
VERSION     DAWHXZ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.315"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..844)
                     /locus_tag="WIE35_002044"
     CDS             complement(<1..844)
                     /locus_tag="WIE35_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1655 family transposase"
                     /protein_id="HEZ4154914.1"
                     /translation="MSYTQLTQGERYHIQYLSRHCTVTEIAKQLNRHKSTISREIRRH
                     RTQGQQYSAEKAQRQSRTIKQRKRQPYKLDSQLIQHIDTLIRRKLSPEQVCAYLCKHH
                     QITLHHSTIYRYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVGIENRPA
                     IVDQKSRIGDWEADTIVGKGQKSALLTLVERVTRYTIICRLDSLKAEDTARAAVRALK
                     AHKDRVHTITMDNGKEFYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPK
                     QTDFR"
BASE COUNT          144 a          189 c          287 g          291 t
ORIGIN      
        1 tacggaaatc ggtttgtttg gggaagtatt gccggatgag tccgttggtg ttctcattca
       61 gccctttctc ccaagaatgg taagggcgac aaaaataagt ctccgctttc aatgctttgg
      121 ttattttggt gtgttggtag aactctttgc cgttatccat ggtaatggtg tgcaccctgt
      181 ctttatgtgc ctttaatgcc ctaacagctg cccgggcagt gtcttcggct ttgaggctat
      241 ccaatctgca gatgatggtg tagcgggtaa cgcgttcgac caaggtcaat aatgcgcttt
      301 tctgtccttt gccgacaatg gtgtcggctt cccaatcgcc gatacgggat ttctggtcga
      361 cgatagcggg tcggttttct atgccgacac ggttggggac tttgcctctg gtccatgtgc
      421 tgccgtagcg tttgcggtag ggtttgctgc atattctgag atgttgccac aacgtgctgc
      481 cgttgctttt gtcttggcga aggtagcggt aaatggtgct gtggtggagc gtgatctggt
      541 ggtgtttgca caggtaggcg catacttgtt cgggactgag tttgcggcgg ataagggtgt
      601 cgatgtgctg aatcagctgc gaatcgagct tatagggttg tcgcttacgc tgtttgatag
      661 tccggctttg ccgctgggct ttttcggcgc tgtattgctg cccttgggtg cggtgccgtc
      721 tgatttcgcg gctgatggtg cttttgtggc ggttcagctg tttggcgatt tcggtgacgg
      781 tgcagtggcg ggacaggtat tggatgtggt atcgttcgcc ttgggtcagt tgcgtgtagc
      841 tcatggcaat ctttcttgca ggaaaggccg tatgctaccg catactggcc tttttctgtt
      901 agggaaagtt g
//
LOCUS       DAWHXZ010000316          889 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.316, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000316 DAWHXZ010000000
VERSION     DAWHXZ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.316"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..720
                     /locus_tag="WIE35_002045"
     CDS             <1..720
                     /locus_tag="WIE35_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ4154915.1"
                     /translation="KGILIALYHIALPMLESLIVLNPDDKDELTDKYGIKIKNIHILG
                     GIGLDSRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDDFIRAAEQVKDKYPDTVFTA
                     LGAIDKSRGGGDLERLAARDIIRFPGFVNNVSEVIKAHHIFVLPSYREGVPRSTQEAM
                     AVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEKMIYFIENREAVRLMGNASYA
                     IAKDKFDAEKVDLKLLDILKA"
BASE COUNT          233 a          206 c          222 g          228 t
ORIGIN      
        1 aaggggattt tgattgcctt gtaccacatt gccctgccga tgttggaaag cctgattgta
       61 ttaaaccccg acgacaaaga cgaactgacg gacaaatacg gcatcaaaat aaaaaacatc
      121 catattttgg gcggaatcgg tctggattcg cggcaatatc cttattccga ggcggatatt
      181 cccgatgaaa aagaacccgt aaaattcctc tttatcggca gatttctgaa agaaaagggg
      241 attgatgatt ttattcgggc ggcggaacag gttaaggaca aataccccga tacggttttt
      301 accgctttgg gcgcaatcga caaatcacgc ggggggggcg atttggaacg gcttgccgcc
      361 cgcgatatta tccgtttccc cggttttgtg aacaatgttt ccgaagtgat aaaggcgcat
      421 catatattcg tattgccgtc ttatagggaa ggcgttcccc gaagcactca ggaggcaatg
      481 gccgtcggca gggcagtgat tacgacggat gtccccggat gcagggaaac ggtcgccgac
      541 aaggtcaacg gcttcctgat cgagccttgg aatccccgca tcttagccga aaaaatgatt
      601 tattttatcg aaaacaggga agccgtccgc ctgatgggga atgcaagtta tgcgattgcc
      661 aaagataaat tcgatgccga aaaagtcgat ttgaaattgc tcgatatttt gaaggcgtaa
      721 acaaggctgc ccgcttttga gtttcggtca tttctgataa acccccgtca ttcccgccac
      781 acccccgtca ttccctcgaa agcgggaatt caggttcgtt tagtttcggt catttccgat
      841 aaattcttgc aactttgcgt ttctagattc ccactttcgt gggaatgac
//
LOCUS       DAWHXZ010000317          889 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.317, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000317 DAWHXZ010000000
VERSION     DAWHXZ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.317"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(38..817)
                     /locus_tag="WIE35_002046"
     CDS             complement(38..817)
                     /locus_tag="WIE35_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ4154916.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          215 a          286 c          178 g          210 t
ORIGIN      
        1 cgatacatcc gaatatgcca gaccgtctaa acggtattca tacctcccaa tcctgcacgc
       61 gcttcaaatc gttttgcgcc aaagtatccg cgtggcggtt acggctctga tattccctgt
      121 ctttcatgat gctgcttgcc acataattca aatgtgcctt tgccgcctcc gaagcctcgc
      181 ccggccgacg gttcgatatc gcctcataca gcacgcggtg ctgcgccatc agtttcggac
      241 gcggatcttc ttcctgattc agataaataa ggctgctgcg cgtctgccgg tacagcattt
      301 tcaacaaacc gcccgacaaa tggctgaaca acaaattgtg cgccgcatcc gcaatcgtct
      361 gatggaagct gacatcggct tcgctctgat gttccagatt gccgctttcg cacgcctcct
      421 caaacttttc aagccaaaac ccaatccgct tcaaatcggc atccgtccgg cgttctgccg
      481 ccaatgccgc catacagccc tcgatgtggc agctgaaatc aaaaacatcc tgttcccaat
      541 tggaatgctt gcccaaaagc tcctgccaac tttgcaaaaa atcctgctgt ggcttgaccg
      601 aaacataata accgtctccc tgcctcgctt ccaaaacctg acgggcaacc aaaacattca
      661 atgccgacct gaccgacggg cgggacacgc cgaactcttc cgccaaaacg cgttcgggcg
      721 gaatcttgcc cccttccgcg taaacccctt ccgcaatgcg ctcctccaat accgacaata
      781 cctgatcgct gattttctga ggccttacca gtttcattac tcctccttta taaagattcc
      841 ctgcagaacc cttccgaaat atagtggatt aacaaaaatc aggacaagg
//
LOCUS       DAWHXZ010000318          864 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.318, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000318 DAWHXZ010000000
VERSION     DAWHXZ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.318"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(152..>864)
                     /locus_tag="WIE35_002047"
     CDS             complement(152..>864)
                     /locus_tag="WIE35_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154917.1"
                     /translation="LVQQSTDGSDRLYAYQSGNTLNGGLGDDYLYGADGDDLLNGDAG
                     NDSIYSGNGNDTLNGGEGNDALYGYNGNDVLNGGEGNDHLNGEDGNDTLIGGAGNDYL
                     EGGSGSDTYVFGKGFGQDTVYNYHVDKNSDTMHFKGFKAADVHFIRSGSDLVLSASEQ
                     DNVRISGFFYGENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGGN
                     VDANTQSVQQPLLVTPSA"
BASE COUNT          238 a          211 c          168 g          247 t
ORIGIN      
        1 gtactggttt ttgtgaatcc actatattgc tagaagtgca ccgattaagg ttaattaaaa
       61 tacgaccctt atcccaatat taagaaaaac agcaatcaaa ataaacttga ttgctgtttt
      121 tcagtttaag ccatgaatgt gattaggctc cttatgcaga tggcgttacc aataacggct
      181 gctgtacgga ttgtgtattg gcatccacat ttcctcctgt cgcagcagta ttagaaccga
      241 acacagacat agactgtacc aaattattgc cagcattaat atacttggca aaatctggat
      301 tactgatagc tgcatcatca aagacaaatg tatctacacg atggttttca ccatagaaga
      361 atccggaaat acgtacgttg tcttgttcag aagcgctaag caccaaatca cttccggaac
      421 ggataaaatg aacatctgct gctttaaatc ctttaaagtg catagtgtca gagtttttat
      481 ccacatggta attatagacc gtatcctgac cgaagccttt gccgaagaca taagtatccg
      541 aaccgctgcc gccctccaag taatcattgc ctgcaccgcc gattagagtg tcgttaccgt
      601 cttcgccgtt caaatgatca ttgccttcgc caccattcag tacatcgtta ccattatagc
      661 cgtacagggc gtcgttgcct tctcctccat tgagcgtatc attgccattg ccactgtaga
      721 tactgtcgtt gcctgcatca ccattcagca ggtcatcccc gtcggcaccg tacagatagt
      781 catcgcccaa tccgccattt aaggtatttc cggattggta ggcatacaat ctgtccgaac
      841 cgtcggtgga ttgctgtacc agtt
//
LOCUS       DAWHXZ010000319          859 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.319, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000319 DAWHXZ010000000
VERSION     DAWHXZ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.319"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            33..>859
                     /gene="mafB"
                     /locus_tag="WIE35_002048"
     CDS             33..>859
                     /gene="mafB"
                     /locus_tag="WIE35_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ4154918.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISESSNIAVMHGLGLLSTENKMARI"
BASE COUNT          221 a          252 c          214 g          172 t
ORIGIN      
        1 tttcattccg taccatctta aggaacatca aattgggcat ttcccgcaaa atatccctta
       61 ttctgtccat actggcagtg tgcctgccga tgcatgcaca cgcctcagat ttggcaaacg
      121 attcttttat ccggcaggtt ctcgaccgtc agcatttcga acccgacggg aaataccacc
      181 tattcggcag caggggggaa cttgccgagc gcagcggtca tatcggattg ggaaacatac
      241 aaagccatca gttgggcaac ctgatgattc aacaggcggc cattaaagga aatatcggct
      301 acattgtccg cttttccgat cacgggcacg aagtccattc ccccttcgac aaccatgcct
      361 cacattccga ttctgatgaa gccggtagtc ccgttgacgg atttagcctt taccgcatcc
      421 attgggacgg atacgaacac catcccgctg acggctatga cgggccacag ggcggcggct
      481 atcccgctcc caaaggcgcg agggatatat acagctacga cataaaaggc gttgcccaaa
      541 atatccgcct caacctgacc gacaaccgca gcaccggaca acggcttgcc gaccgtttcc
      601 acaatgccgg cgctatgctg acgcaaggag taggcgacgg attcaaacgc gccacccgat
      661 acagccccga gctggacaga tcaggcaatg ccgccgaagc cttcaacggc actgcagata
      721 tcgtcaaaaa catcatcggc gcggcaggag aaattgtcgg cgcaggcgat gccgtgcagg
      781 gtataagcga aagctcaaac attgctgtta tgcacggctt gggtctgctt tccaccgaaa
      841 acaagatggc gcgcatcaa
//
LOCUS       DAWHXZ010000320          810 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.320, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000320 DAWHXZ010000000
VERSION     DAWHXZ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.320"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..419
                     /locus_tag="WIE35_002049"
     CDS             <1..419
                     /locus_tag="WIE35_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ4154919.1"
                     /translation="DGKAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIAGLVGIAP
                     HPRESGETKFKSRCFGGRSAVRKALYMATVAATRFEPLIRDFYQRLPSEGKPYKVAVT
                     ACMRKLLTISNARMRDYFAENDTAENDTAENGIRTA"
BASE COUNT          185 a          182 c          230 g          213 t
ORIGIN      
        1 ttgacggcaa agcccaagtg gcagaacaaa tcaaaggcat cggttcgata acgacggcta
       61 cgctgatggc aatgctaccc gaattggggc ggctgtcgca caaacggata gcgggtttgg
      121 tcggcattgc cccgcacccg agggagagcg gggaaaccaa attcaaaagc cgctgctttg
      181 gcggaaggtc tgcggtgcgt aaggcactgt atatggctac cgtggcagcg acacgttttg
      241 aaccgcttat tcgggatttc taccaacgcc tgccgtccga gggtaagccg tataaggttg
      301 ccgttacggc atgtatgcgc aaactgctga cgatatcgaa tgcccggatg cgtgattatt
      361 ttgccgaaaa cgataccgcc gaaaacgata ccgccgaaaa cggtatccgg acggcttgat
      421 ttgagttttg gtatttttgc ccgacggggt gaaaaataca gttgctacgg ctcgccgaat
      481 catcagaaat acccgaaccg tcattcccgc gcaggcggga atccagaccg gtcggtgcgg
      541 aaacttatca ggtaaaacgg tttcttgaga tttttcgtct tggattccca ctttcgtgtg
      601 aatgacgtgg tgcaggtttc cgtatggatg gattcgtcat tcccgcgcag gcgggaatct
      661 agtctgttcg gtttcagtta ttttcgataa atgcttgttg cttttcattt ctagattcac
      721 actttcgcgg gaatgacgaa ttttaggttt ctgattttgg ttttctgtcc ttgtgggaat
      781 gacgggatgt agattcgtag gaatgacgtg
//
LOCUS       DAWHXZ010000321          788 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.321, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000321 DAWHXZ010000000
VERSION     DAWHXZ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.321"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            187..663
                     /locus_tag="WIE35_002050"
     CDS             187..663
                     /locus_tag="WIE35_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ4154920.1"
                     /translation="MTGFNDAAGVASSPSDIKGKYVQSVTVANGVITAQMASSNVNKE
                     IKDKKLSLWAKRQDGSVKWFCGQPVQRANANGANNDEVTAATGKGTDKIDTKHLPSTC
                     RDASSAVCIETPRADFKHFQKISRHRVLPESRHMAEKLRHSRAGGNLGLSVRKLIG"
BASE COUNT          216 a          229 c          189 g          154 t
ORIGIN      
        1 caccacgcgc cgacttcaaa cacttccaaa aaatcagccg ttaccgagta ttgcccgaat
       61 cacggcacat ggccgaaaaa cttcgtcatt cccgcgcagg cgggaatcca ggtctgtcgg
      121 tacggaaact tatcgggtaa aacggtttct tgagattttg cgttctggat tcccactttc
      181 gtgggaatga cgggatttaa tgatgccgcc ggcgtggcat cctccccctc cgacatcaaa
      241 ggcaaatatg ttcaaagcgt tacagtcgca aacggcgtca ttaccgccca aatggcttca
      301 agcaacgtaa acaaagaaat caaagacaaa aaactctccc tgtgggccaa gcgtcaagac
      361 ggttcggtaa aatggttctg cggacagccg gttcagcgcg ccaacgccaa cggcgccaac
      421 aacgacgaag tcaccgccgc caccggcaag ggcaccgaca aaatcgacac caagcacctg
      481 ccgtcaacct gccgcgacgc atcatctgcc gtttgcatag aaacaccacg cgccgacttc
      541 aaacacttcc aaaaaatcag ccgtcaccga gtattgcccg aatcacggca catggccgaa
      601 aaacttcgtc attcccgcgc aggcgggaat ctaggtttgt cggtgcggaa acttatcggg
      661 taaaaaggtt tctccggccc tgagttctag attcccactt tcgtgggaat gacgggattt
      721 aatgatgccg ccggcgtggc atccgcctcc gacatcaaag gcaaatatgt tgagaaagtt
      781 gaagtcaa
//
LOCUS       DAWHXZ010000322          780 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.322, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000322 DAWHXZ010000000
VERSION     DAWHXZ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.322"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..115
                     /locus_tag="WIE35_002051"
     CDS             <1..115
                     /locus_tag="WIE35_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ4154921.1"
                     /translation="GQWGANQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            180..734
                     /locus_tag="WIE35_002052"
     CDS             180..734
                     /locus_tag="WIE35_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ4154922.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TVGTIAMARTTDPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVE"
BASE COUNT          214 a          201 c          196 g          169 t
ORIGIN      
        1 cgggcaatgg ggtgcgaacc aagtctcgtg gcggcgcgat tcgcgttttg aattgattgc
       61 caaatcgttt gatgcggatt taatcgaaca aaagctcaaa gacgcattgg aatagcgttc
      121 acgcgccttc agacggcata tgaaatgaaa atgccgtctg aaggtctgag gaggacggga
      181 tgaaacccaa attcaaaacc gttttaaccg cgctgctttt ggcggtttcc ctgccgtcta
      241 tggcggcaac ccatgttttg atggaaaccg atatgggcaa tatccgtttg gttttggacg
      301 aatccaaagc acccaaaacc gttgccaatt tcgtgcgcta tgcccgaaaa ggcttttacg
      361 acaatacgat ttttcaccgc gtcatcggcg gcttcgttat ccaaggcggc ggattgaccg
      421 aggacttggc acaaaaggca agcgataagg ccgttgccaa cgaatccggc aacggcttga
      481 aaaacactgt cggcaccatc gccatggcgc ggacgacaga ccccgattcc gccaccagcc
      541 aattctttat caatctggcg gacaatgatt cactcaacta caaaaacgga caatacggct
      601 ataccgtttt cggcagggtc gaaagcggaa tggacaccgt ttccaaaatc gcccgcgtca
      661 aaaccgccac gcgcggcttt tatcaaaacg tacccgtaca gcccgtcaaa atccgtcgcg
      721 ttgttgtcga gtaatataac gggaaacctt cagaaagctg aaacggaaaa agcaggtgtt
//
LOCUS       DAWHXZ010000323          779 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.323, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000323 DAWHXZ010000000
VERSION     DAWHXZ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.323"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            247..>779
                     /locus_tag="WIE35_002053"
     CDS             247..>779
                     /locus_tag="WIE35_002053"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEZ4154923.1"
                     /translation="MASKPFLKWAGGKHKLVPFIEHNLPTPTRKRLIEPFCGSAALSL
                     ALDFEQYLLNDINADLIGLFRILKEEKSGFIDYARSFFISENNSDSRFYELREQFNFS
                     KNLHERSALFIYLNRHAFNGLCRYNSKGVFNVPFGRYKSPYFPQQEMEGFIQKSDRVE
                     LMCGDFQMILSQTDNTDT"
BASE COUNT          230 a          177 c          148 g          224 t
ORIGIN      
        1 gtgggaattt aggggatttt ggggaatttt gcaaaggtct caggccgtct gaaacttgga
       61 aacagggttt cagacggcct ttttaaggca gatggagttg ataagtccaa taacatattg
      121 gaatggaaag gttctattgg atttagactc actctacaca cgcccccaaa tataaatcca
      181 ttgtattcct cacgatgttt tggcacaatt ccaagccaat caaccacgct tgtactcggc
      241 atcctaatgg cttcaaaacc ttttctaaaa tgggcaggcg gcaaacataa acttgtcccc
      301 tttatcgaac acaatctacc tactcccaca cgaaaacgcc tgattgaacc cttttgcggt
      361 tctgccgctt tgtctcttgc attggatttc gaacaatatc tgctcaatga tattaacgct
      421 gacttaatcg gcttattccg tatcctaaaa gaagaaaaat ccggattcat cgactacgcc
      481 cgctctttct ttatttccga aaacaatagc gattcaagat tctatgaatt gcgggaacaa
      541 ttcaacttca gcaaaaatct gcacgaacgc tcggcactat ttatttattt gaaccgtcat
      601 gcttttaacg gtttatgccg ttacaacagc aaaggggtat tcaatgtgcc gttcggacgc
      661 tacaaatcgc cgtactttcc ccaacaagaa atggaaggtt tcattcagaa atctgacaga
      721 gttgaattaa tgtgcggaga ttttcaaatg attctatctc agacagacaa taccgatac
//
LOCUS       DAWHXZ010000324          775 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.324, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000324 DAWHXZ010000000
VERSION     DAWHXZ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.324"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            504..>775
                     /locus_tag="WIE35_002054"
     CDS             504..>775
                     /locus_tag="WIE35_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ4154924.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLAEGQKSAVTEYYLNHGEWPSDNSSAGVASSPSDIKGKYVQKVEV"
BASE COUNT          266 a          211 c          123 g          175 t
ORIGIN      
        1 ccatccgcac ggaaacctgc cgcgatgatt caactgccgt ttgcacagga acaccacgcg
       61 ccgatttcaa acactttcca agaaaacgga aatttttaaa aattaaattt aaaatttccc
      121 acccaaccca ccctatgcta ccgcgaaaat tcaaaaatct caaattccga cccaatcaac
      181 acacccgatg ccccatgcca atagagatac cccacgccaa taaaaaagtg ccgaaaatcg
      241 gcactaaaac tgacaatttt caacactgct gacaaaaaac ggtaatcccc tgcttcctca
      301 aaccacaccc aaacgaaaga aaatgtaaaa taaaaacaac catataaaga taaacgcata
      361 aattttcacc tcaaaacaca aacttggtac caatcttgct ttataataca caggtatcgc
      421 aaaaaaatac cgatggtcaa atacattgca taatgccgat ggcgtaagct tgaagcattt
      481 cctttccaat taggagtaat tttatgaaca cccttcaaaa aggttttacc cttatcgagc
      541 tgatgattgt gattgccatc gtcggcattt tggcggcagt cgcccttcct gcttatcaag
      601 actacacagc ccgcgcacaa gtttccgaag ccatcctttt ggctgaaggt caaaaatcag
      661 ccgtcacaga gtattacctg aatcacggcg aatggccatc cgacaatagt tctgccggcg
      721 tggcatcctc cccctccgac atcaaaggca aatatgttca gaaagttgaa gtcaa
//
LOCUS       DAWHXZ010000325          753 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.325, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000325 DAWHXZ010000000
VERSION     DAWHXZ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.325"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            44..>753
                     /locus_tag="WIE35_002055"
     CDS             44..>753
                     /locus_tag="WIE35_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ4154925.1"
                     /translation="MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMAT"
BASE COUNT          199 a          196 c          204 g          154 t
ORIGIN      
        1 tagaatggga aggtacagat tgtttgaaga ttaggggacg aggatgttta ccgatgaaaa
       61 tatgaccgca aaggaagaac tgttcgcatg gctgcgccat atgaacaaaa acaaaggttc
      121 cgacctgttt gtgacgaccc atttcccgcc cgcaatgaag ctggacggca aaatcacccg
      181 catcacggac gaaccgctga cggcggaaaa atgtatggaa atcgcctttt cgattatgag
      241 tgcgaagcag gcggaagaat tttcatcgac caacgagtgc aacttcgcca tcagcctgcc
      301 ggacaccagc cgcttccgcg tcaatgcgat gatacagcgc ggcgcgacgg cgttggtatt
      361 ccgtgcgatt accagcaaga ttcccaagtt tgaaagcctg aacctgccgc cggtcttgaa
      421 ggatgtcgcg ctgaaaaaac gcgggctggt tatttttgtc ggcggcaccg gctcgggcaa
      481 atcgacttcg cttgcctcgc ttatcgatta ccgcaatgaa aattcgttcg gacacatcat
      541 caccatcgaa gacccgatcg agtttgtcca cgaacacaaa aactgcatca tcacccagcg
      601 cgaggtcggc gtggatacgg aaaactggat ggcggcgttg aaaaacacgc tgcgtcaggc
      661 gccggatgtc atccttatcg gcgaaatccg cgaccgcgaa acaatggact acgccattgc
      721 ctttgccgaa acggggcatt tgtgtatggc gac
//
LOCUS       DAWHXZ010000326          747 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.326, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000326 DAWHXZ010000000
VERSION     DAWHXZ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.326"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            13..693
                     /locus_tag="WIE35_002056"
     CDS             13..693
                     /locus_tag="WIE35_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ4154926.1"
                     /translation="MQADLAYAAERITHNYPEPTGADKDKISTVSDYFRNIRAHSIHP
                     RVSVGYDFGDWRIAADYASYRKWKESNSSTNAENRDNAKNYVKIETKHQGNGSFHAAS
                     SLGLSAIYDFKLNDKFKPYIGARVAYGHVKHQVHSVETKTTTVTSKPTATSPQGGPII
                     QTDPSKPPYHESHSISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEV
                     SLGMRYHF"
BASE COUNT          221 a          213 c          153 g          160 t
ORIGIN      
        1 agcccgtatt atgtgcaggc ggatttagcc tatgccgccg aacgcattac ccacaattat
       61 ccggaaccaa ccggtgcaga caaagacaaa ataagcacag taagcgatta tttcagaaac
      121 atccgtgcgc attccatcca ccctcgggtg tcggtcggct acgacttcgg cgactggaga
      181 atagcggcag attatgccag ttacagaaaa tggaaagaaa gtaattcttc tactaatgca
      241 gaaaatagag ataatgcaaa aaactacgta aagattgaaa caaaacatca aggaaacggc
      301 agcttccacg ccgcttcttc tctcggctta tccgccattt acgatttcaa actcaacgat
      361 aaattcaaac cctatatcgg cgcgcgcgtc gcctacggac acgttaaaca tcaggttcat
      421 tcagtggaaa caaaaaccac gactgttacc tctaaaccga cggcaacctc tccacaggga
      481 ggccctatta tacaaactga tcccagcaaa cctccctatc acgaaagcca cagcatcagc
      541 agcttgggtc ttggtgtcat cgccggtgtc ggtttcgaca tcacgcccaa gctgaccttg
      601 gacaccggat accgctacca caactgggga cgcttggaaa acacccgctt caaaacccac
      661 gaagtctcat tgggcatgcg ctaccacttc tgattccccg ataccgatgc cgtctgaacc
      721 ttcagacggc atttttgatt tacctgc
//
LOCUS       DAWHXZ010000327          747 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.327, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000327 DAWHXZ010000000
VERSION     DAWHXZ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.327"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          142 a          219 c          211 g          175 t
ORIGIN      
        1 agcgcggtat ggcggaaaac ctgccttttt aaagttttat tcatcgtatt tccttttcgg
       61 tcaaaccccc cgcccttcgg gcatccgtcc ttcggggcgg caggatcagg ctttgtccgg
      121 gaggggtgca agcccttccg aatcagggcg gcgccttatg tgccgccctg cgtgttggaa
      181 catattttat cccttaaatc ttgcttcgcg ggataccgtc ggcggcaggc gcgccgcaaa
      241 cggcgggatt cggcaaaccg cccgattccg cgcccgccga attgctggtt ggaaaacccc
      301 tttggcccgg ctgccaaagg gggatgttcg gaaaagtaac acgccccttt ggccggatgc
      361 aatgtatagg gttaccgcat tattctaccc ttgcgcgctg atcatggtaa agcggttttc
      421 ggcaatccgc aaccgcctcc ttggcccgcc attgccgcga aagcgggaat ccggaacgta
      481 aaatctcaaa aaaaccgttt tatccggcaa gtttgcccgc cggcaggccc gccattgccg
      541 cctgcgcggc aatggcgggc cggggaggcg gcattttcca agttgcggca atctttccca
      601 acaactcaac cgccccaata caagcctttc ggcttgttgc cctctctcta gctctctccc
      661 acagggagag aggacgggtc ggctgttggg gttgaggttt gctgcaagga aaacaggttg
      721 tgcaggctgc tttttatttt tcagacg
//
LOCUS       DAWHXZ010000328          719 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.328, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000328 DAWHXZ010000000
VERSION     DAWHXZ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.328"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(172..690)
                     /locus_tag="WIE35_002057"
     CDS             complement(172..690)
                     /locus_tag="WIE35_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154927.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVFTGKEESMLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARNALPSNQTYQQHQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
BASE COUNT          161 a          210 c          142 g          206 t
ORIGIN      
        1 tgttaatcca ctataatttc cgcccgaaca aaaataccct ttgcaaacgg acggaatcaa
       61 acgggtattc aaacgcgtcc gaacaaaaaa agcggacatt cctgcccgcc cgtaccggta
      121 cagaaaccgc acgtccgatt cggacggcgg catcctcccg gttgtcctac gctaaaacaa
      181 caatgtcggt ttgccgttgc atttgatttc tttttccaat tcgtcaaact cggcttcaaa
      241 ccgccgtttc aactgctcga tcgccgcctg atgctgctga tacgtctggt ttgacggcaa
      301 cgcatttcgc gcgtcgagat atgcctgtgc cgtttgtccg cactttttct gatgggcgat
      361 gattttgtcc ttcatcgccg catcggtttt gacatactgc ctgcgttcga catacagctc
      421 tttcccgtca tcggaaagtt tgatcgggat ttcccctatc cccgtgttta tcgaaagcgc
      481 gccgtctttt tcagacaaaa gcatagattc ttccttacct gtaaacacgt tgattttatt
      541 aaggaagtaa ttgccttttt cttttttgac ggcaatcata ccctcttttt tgtccgattt
      601 ttcgcgccaa taaccctcgt aaccgccggc atccttgccg cagcccgcca acaccgccac
      661 cacgccgacg gctaaaagca tttgtttcat ttccatactc cgttaacatt aagaccgag
//
LOCUS       DAWHXZ010000329          710 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.329, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000329 DAWHXZ010000000
VERSION     DAWHXZ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.329"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(38..604)
                     /locus_tag="WIE35_002058"
     CDS             complement(38..604)
                     /locus_tag="WIE35_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ4154928.1"
                     /translation="MGFYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWVAFVLLGGLGLKMMREGLSGEAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTAGRALGVLF
                     GRCAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          205 a          244 c          136 g          125 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactatattt tccgacatca ttgaatcaaa cccaaatgcg
       61 acaagagcgt ccatgtgccg atggcaatca acaccaaacc tccggcaaat tccgcacacc
      121 tgccgaacaa tacgcccaaa gcccttcccg ccgtcagccc gaccgccacc atcaccgtcg
      181 tcgccatacc gatgattgcg gcggcaaagg cgatgtttac ctccataaac gccaagccca
      241 ccccgactat catggaatca atactggttc caaaagcagt caaaaccgtc atccataggc
      301 tttcccgttt gctttcgcgc acatcttccg cctcgccgga cagcccttcg cgcatcattt
      361 tcagacccag cccgcccagc aggacgaaag ccacccaatg gtcccattcg ctgataaacg
      421 gcttggcata aaaaccgcct acccagcctg ccagcggcgt gagcgcttca accgtgccga
      481 acaccaaagc cgttgccgca attttgcgcg gaggcattct gaccgccgcg ccctttgcca
      541 atgcgacggc aaacgcatcc atcgacatcc ccagagcaat caagagcaaa gcataaaaac
      601 ccataccgca cccgtcctca aaaagggcgg attatagcaa aagcaaaaaa atgcaaaaat
      661 gccgcacgaa aacccgcatc ccgtcattcc cgcaaaaaca aaaaatcaaa
//
LOCUS       DAWHXZ010000330          701 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.330, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000330 DAWHXZ010000000
VERSION     DAWHXZ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.330"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          207 a          193 c          148 g          153 t
ORIGIN      
        1 tgtactggtt tttgttaatc cactatatcc gtttgcgccc gccaagccgt gttgcacaga
       61 tgcgacacgg cttttttctt tctgttgtca gaatgtaaat tttcgtcaaa aaacatcaaa
      121 aatcaccccc cccccgaatt tgcgtaaaat aaagtgtaat ccctttgatc ataagggaat
      181 gttgacaatt agatcgattc tcctaggctg actttcgcaa taaatggggt tgtcaataat
      241 tgtgtatggt cgtttgccgt ccggcggccg tcattcccgc gcaggcggga atccagaaac
      301 gcaacgcagc aggactttat cggaaaaaac cgaaatccca ccgccgtcat tcccgcgcag
      361 gcgggaatcc agcaacacaa cgcagcagga ctttatcgga aaaaaccgaa accccaccga
      421 ccgtcattcc cgcaaaagcg ggaatccaga cccgtcggca cggaaactta ccggataaaa
      481 cagtttcctt agattccacg tcctagattc ccgccttcgc gggaatgacg agattttaga
      541 ttatgggaat ttatcaggaa tgattcaatc catagaaaaa ccacaggaat ctatcagcaa
      601 aaacagaaac ccccaccgcc gtcattcccg cgcaggcggg aatccagcaa cacaacgcgg
      661 caggacttta tcggaaaaaa cagaaacccc accgaccgtc a
//
LOCUS       DAWHXZ010000331          698 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.331, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000331 DAWHXZ010000000
VERSION     DAWHXZ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.331"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..698)
                     /locus_tag="WIE35_002059"
     CDS             complement(join(<1..479,479..698))
                     /locus_tag="WIE35_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242698.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ4154929.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQKFFFDLRKEIDKEWVFIDGSYVRCHQHAS
                     GARRGFDRAIGQSRGGNTTKIHLCVDSHGNPLDFKVTGGNVHDSQVANDLIEVIQEAQ
                     YFIADKGYDSQEIRDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLK
                     QFRSIATRYEKLARN"
BASE COUNT          161 a          161 c          136 g          240 t
ORIGIN      
        1 attacgagct aatttctcat agcgagtagc aatactacga aactgcttta atcgtgcaaa
       61 tgcattctcg actaagtggc gtaatttata aaggtagcta tcaaaatcag ggttaggttt
      121 cttggcattt ttacgctttg gtataatagc tttcataccg tgttctatcg ctttatctct
      181 gatttcttgc gagtcatacc ctttgtcagc gataaaatac tgggcttctt gtatgacttc
      241 tatcaagtcg tttgcaactt gactgtcgtg cacgttaccc ccagtgactt taaaatcgag
      301 cggatttcca tgcgagtcca cacataggtg tatttttgtc gtgtttccgc cacggctttg
      361 tccaattgct ctatcgaaac cacgccgagc tccacttgca tgttgatgac accgtacata
      421 acttccgtcg atgaataccc attctttgtc aatttctttt cgtagatcaa aaaaaaattc
      481 tgccacaagc cttttttaga ccatcggtta aagcggttat aagcggtttt ccatgaccct
      541 agctcaatag gtatgtctcg ccatggtgca cctgttctta gcttccatag tatggcttcc
      601 atcacggtac ggtcgttctt ccattgatga cagccgtgcg ctttcatggt tgtttgtaat
      661 tgttcccata tgttgtcagt tattgcggtt ctcgccat
//
LOCUS       DAWHXZ010000332          676 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.332, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000332 DAWHXZ010000000
VERSION     DAWHXZ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.332"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(90..641)
                     /locus_tag="WIE35_002060"
     CDS             complement(90..641)
                     /locus_tag="WIE35_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154930.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDLEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
BASE COUNT          189 a          270 c          125 g           92 t
ORIGIN      
        1 aaagaaaccg ccattagcac tacacccccc caagctgcgg ctgccaaaca catcggctgc
       61 ttctcgacgc acccgctgaa aaatgcgtcc tatccgtccg ccttgcccgc accccctgac
      121 tttgccgccc tgcccgatgc cgtctgaagc ctgaaccgcc ccaccagccc gatgacgcac
      181 gacaccgccg gaaagggcat acacaaagcc gtcagcacat aacgcgcatt ccccgtattg
      241 accacgtccg cgcccttcag cagcagcggt agcgcgttcg cccactccca caccgacggc
      301 gcgctgaacg ccaataccgc caccagcccc aacatccgga acaccccgat ttccaacacc
      361 tgccgccgcc gcaccgaacg gttgagcaaa aacacaaccg ccaacccgaa acagaccacc
      421 gccgccatac cgccgttggc gacaatctgc aaactgttga atgcaatatc gggcgtaacc
      481 gccaacacgc cttccaaatc cggcacggca cgctgcaaat ccaaaccctg caacacattc
      541 aacacaaaac cgtagatcaa atcaaaaaac accacgccta tcggcaaagc actcaaaacc
      601 cgccacatct tcctgtccga ccaaccgttc aaaaccttca ttttacccga ccacgcaagc
      661 cgccgaacaa aaaata
//
LOCUS       DAWHXZ010000333          651 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.333, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000333 DAWHXZ010000000
VERSION     DAWHXZ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.333"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WIE35_002061"
     CDS             complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WIE35_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ4154931.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          194 a          212 c          116 g          129 t
ORIGIN      
        1 tcaaaccgga aaaatgccgt ctgaatcttc agacggcatt ccattcatat ttattcgtct
       61 tttttgtgtt cgatggcgtt tcgggaacga ttgccgccgt aaggctcttc aaccgtatat
      121 tccccctcga taatatcgtc atcgcgggaa aagccctctt ttctgcccga ttggttcatg
      181 ttgaaaaaat tttccgcacc tcctgcctgc aacactgcac ctcccttaaa cggcagcagc
      241 agcaacaccg ccaacaccga ggatacgaat cccggactca tcagacacac cgccgccacc
      301 gtataacgga taggccacaa catctgataa acggataccc tcccgccgct tctcattgcc
      361 gcgcccgcca ataaaagacc ggacagcccc gtatgcctga gcatcagcac accggcggca
      421 aaacctgccg ccatcaaaaa caacgtccag ccgccgccca accaatcggc aacccacaca
      481 atcgacataa tctccaaaaa cagcagcacc aaaaaaccga taccgaaaaa tctcattgac
      541 cgtcatcctt atatttaagt aaacagcaaa ccgcccgaac aggactccaa gcgacctgcc
      601 tgtaaatgat tacaaaacca tgtgcttcaa gccgaaacaa tgtgaaatca c
//
LOCUS       DAWHXZ010000334          650 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.334, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000334 DAWHXZ010000000
VERSION     DAWHXZ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.334"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            281..487
                     /locus_tag="WIE35_002062"
     CDS             281..487
                     /locus_tag="WIE35_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154932.1"
                     /translation="MFDGAKVVRRMEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSV
                     YLKQTGKGSNILQSSYSHKRFIVN"
BASE COUNT          201 a          107 c          175 g          167 t
ORIGIN      
        1 aataggggga agtctgcagc cgcatcaaat gccgtctgaa catgtgttcg ggcggcgttt
       61 tgttgggggg tatcggagtg gaacgtctga aaaagggttt caggcggtct ttgggcgtgt
      121 ggtgacagtc gaaaacgtga taaggctacc tgaaaagttt gggagatttt caggtagcct
      181 ttggtattgg gcgcaacaga cgcaggtaca gattaggcgg tgtgccgtaa tcgtacgaat
      241 gccgattcaa cctaagcaga catcagtatt taggaagtgg atgtttgatg gagcaaaggt
      301 tgtacgaagg atggaaggca acctgtgggt gtttggtatg gtcgcgcttg aaaaaacgtg
      361 ttttaaggga caaatgccgt ctgaaaatcg gtttcagacg gcattttctg tttatttaaa
      421 gcaaacagga aaaggcagca atattctgca gtcttcctat tcacacaagc gttttatagt
      481 taattaaaaa caaaatagta caatactcaa ctttgaaggt ctaaccatgg catactctgc
      541 ggacttaaga aacaaagctt taaactaggg gctgtactag attagcagat atgttaccct
      601 cgaaatatga agataacgca ctgcaaatta aagaaaaaag tacagaaaga
//
LOCUS       DAWHXZ010000335          649 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.335, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000335 DAWHXZ010000000
VERSION     DAWHXZ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.335"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            22..>649
                     /locus_tag="WIE35_002063"
     CDS             22..>649
                     /locus_tag="WIE35_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ4154933.1"
                     /translation="MFGFRLSGGKGILQRSQVALVVTESTGGLEIPAAKAIRRAGIAV
                     IIANPRQTHQFAQSQPLTKTDAKDAKMPAFFTQMMTQKEDWQTMPYQPPTEAEEVLEA
                     LVNRRNQSADMRTAEKNRLHQVHETQVGSVKQLIAHFDRLIDELDKQIDDHTHTHFDG
                     KAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIASLAGIAPHPRESEE"
BASE COUNT          182 a          183 c          176 g          108 t
ORIGIN      
        1 gctgaaaaca gctgaaaacc tgtgtttggg tttcggctgt cgggagggaa aggaattttg
       61 caaaggtctc aggtcgcact cgtcgtgacg gaaagtaccg gcggtctcga aatccccgcc
      121 gccaaagcca tccgccgagc aggcatagcc gtgattatcg ccaacccgcg tcagacgcat
      181 cagtttgccc aatcgcagcc gctgaccaaa accgacgcca aagatgccaa aatgcccgcc
      241 ttcttcacac agatgatgac gcagaaagaa gattggcaaa ccatgcccta ccaaccgccc
      301 accgaagcgg aagaagtgtt ggaagccttg gttaaccgcc gcaaccaatc ggcggatatg
      361 cggactgccg agaaaaaccg tctgcatcag gttcatgaaa cgcaagtcgg aagcgtcaaa
      421 caactgattg cccattttga ccggctgatt gacgaattgg acaaacaaat cgacgaccac
      481 acccacacgc attttgacgg caaagcccaa gtggcagaac aaatcaaagg catcggttcg
      541 ataacgacgg ctacgctgat ggcgatgctg cccgaattgg ggcggctgtc gcacaaacgg
      601 atagcgagtt tggccggcat tgccccgcac ccgagggaga gcgaggaaa
//
LOCUS       DAWHXZ010000336          631 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.336, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000336 DAWHXZ010000000
VERSION     DAWHXZ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.336"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(145..235)
                     /locus_tag="WIE35_002064"
     tRNA            complement(145..235)
                     /locus_tag="WIE35_002064"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Ser,seq:gga)
     gene            complement(274..360)
                     /locus_tag="WIE35_002065"
     tRNA            complement(274..360)
                     /locus_tag="WIE35_002065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(324..326),aa:Leu,seq:cag)
     gene            complement(396..482)
                     /locus_tag="WIE35_002066"
     tRNA            complement(396..482)
                     /locus_tag="WIE35_002066"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(446..448),aa:Leu,seq:cag)
BASE COUNT          202 a          140 c          129 g          160 t
ORIGIN      
        1 ataccgcaaa acataccata aaaaaaacgg taacggatag ttgtaagcgg ttgatgacga
       61 ttatagacaa taacgggttt tcccaatgaa attattgttt gaataataaa aaatcccaaa
      121 ccgtaaaagt ttgggatttg tatttggcag agaggaaggg attcgaaccc tcgatacgct
      181 attcacgtat acacgctttc caggcgtgcg acttaaacca ctcatccacc tctctaatgg
      241 cggaaattat cccaatcggg ataatttatt atttggtgcc cgggagaaga ctcgaacttc
      301 cacacccatg aggataccag cacctgaagc tggcgcgtct accaattccg ccacccgggc
      361 aatctaaata ttgaaataaa gcaaagcatt tgatttggtg cccgggagaa gactcgaact
      421 tccacacccg tgaggatact agcacctgaa gctagcgcgt ctaccaattc cgccacccgg
      481 gctttgcttt atttgctgtt ttgcggtaca gtgtcctgcc gcaaagaagt cgctattata
      541 tagttcataa agagaatgtc aacagtccaa atgaataaaa atattaaatc cttaaattta
      601 cgggaaaaag acccgttttt aagtcgtgaa a
//
LOCUS       DAWHXZ010000337          629 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.337, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000337 DAWHXZ010000000
VERSION     DAWHXZ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.337"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..416)
                     /locus_tag="WIE35_002067"
     CDS             complement(<1..416)
                     /locus_tag="WIE35_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEZ4154934.1"
                     /translation="MMHTPSPHNEFIRAIKESSPILIGLLPWALILGMQGGQKGMSWL
                     EMLLMTGMNFAGGSEFATVNLWAEPLPILLIATVTFMINSRHILMGAALALHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKATGLPAFNMP"
     gene            558..>629
                     /locus_tag="WIE35_002068"
     CDS             558..>629
                     /locus_tag="WIE35_002068"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="catalyzes the formation of dUMP from dUTP; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ4154935.1"
                     /translation="MNIEVEMKVLDERMADFIPTYATA"
BASE COUNT          165 a          143 c          164 g          157 t
ORIGIN      
        1 aaaggcatat tgaatgcggg taaaccggtt gctttccgtt tttggatttc ggagaatgcc
       61 atcgcccagc tttcatcaca cataaaaaac agtgcgggca cggctttttt cagcggtatt
      121 tctttcaggt gcagggcaag tgccgccccc atcaggatat gccgcgaatt aatcataaag
      181 gttacggtgg cgataagcag tatcggcaga ggttccgccc acaggttgac cgtggcaaac
      241 tcggagccgc cggcgaagtt cataccggtc atcaacaaca tttccagcca gctcatgcct
      301 ttttgtccac cctgcatacc gagtattaat gcccaaggca gaagtccgat aagtatgggc
      361 gaactttctt tgatggcgcg tataaattcg ttatgcgggg aaggtgtgtg cataatgttc
      421 gtcttcataa cgggaagggc gggcattata cactggcaac ggatttcaaa acaaaaccga
      481 tttgccgtgt ttcagcgtaa acacggcttg tgtataatct cccatctttg aaaccggccg
      541 tatgcaggag caagacgatg aatattgaag tagaaatgaa agtgttggac gagaggatgg
      601 cggattttat ccctacctat gcgacagcg
//
LOCUS       DAWHXZ010000338          629 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.338, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000338 DAWHXZ010000000
VERSION     DAWHXZ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.338"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(294..>629)
                     /locus_tag="WIE35_002069"
     CDS             complement(294..>629)
                     /locus_tag="WIE35_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEZ4154936.1"
                     /translation="TDKSARFLAMEIVREKLFRYLGEELPYAMNVEVEQFEEEDGLNR
                     IYIAVLVDKESQKAILIGKGGERLKKISTEARLDMEKLFDTKVFLKVWVKVKSGWADD
                     IRFLRELGL"
BASE COUNT          178 a          165 c          125 g          161 t
ORIGIN      
        1 aatgacggct gcagatgccc gacggtcttt atagtggatt aacaaaaatc aggacaaggc
       61 ggcaaagccg cagacagtac agatagtacg gaatcgattc acttggtgct tcagcacctt
      121 agagaatcgt tctctttgag ctaaggcgag ccaacgccgt actggttttt gttaatccac
      181 tataatatgc acagataata tcaacccgtt tttaacaaag atattcccga ttgaaacaaa
      241 atgccgtctg aaacctgttc ggacggcatt tgcgtaaagt tcagcaagaa aaactacaaa
      301 cccagctcgc gcaggaagcg gatgtcgtcc gcccaaccgg atttgacttt gacccagacc
      361 ttcaaaaata ctttggtatc aaacagtttt tccatatcca accgcgcttc ggtggaaatc
      421 ttcttcaaac gctccccgcc tttgccgatt aaaatcgcct tttggctttc cttatcgacc
      481 aaaacggcga tgtagatgcg gttcaaaccg tcttcctctt caaactgctc cacttcgaca
      541 ttcatcgcat aaggcaattc ctcgcccaaa tagcggaaga gtttttcacg cacgatttcc
      601 atcgctaaaa aacgcgccga tttgtccgt
//
LOCUS       DAWHXZ010000339          620 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.339, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000339 DAWHXZ010000000
VERSION     DAWHXZ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.339"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..275)
                     /locus_tag="WIE35_002070"
     CDS             complement(<1..275)
                     /locus_tag="WIE35_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ4154937.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGGTSAPDFNAG
                     GTGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDA"
BASE COUNT          156 a          128 c          143 g          193 t
ORIGIN      
        1 gcatccctgt ctgtaaccgc aacgtcatcc cgaccggcac agagcatgct tctgtctttg
       61 cacatttcgt tcttgatacc ggcgtaagat actgctgctg atttcgctgt tgttgccctg
      121 ctgttgctgc cgataccggt accgcctgca ttgaagtcgg gcgcagaagt gccgcctccg
      181 ccgccgccta agcaggcaga aagtgttgtt gcaacagcta acgccatggc agccggtttg
      241 aaagtttttg tagggaaggt tggggtcgtt cgcatgatga ggtctccttg aatctgacaa
      301 tagaattcag gcgagggaat aaaaattccg taatgtttag atgtaacccg tttttatatt
      361 ttgaattata ttattatagg ggttgtcttg gacaactagg ataaactcga ttttactaat
      421 tgttttaaaa tggaaatttg aactttcgac aaatagctca atgagtttat tttgtttata
      481 ccggcttaga cggctttttc tcatagggat aattctaact taatttgaat ttccctagtt
      541 atagtggatg aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag agaacgattc
      601 tttaacaagt gaatcggttc
//
LOCUS       DAWHXZ010000340          598 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.340, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000340 DAWHXZ010000000
VERSION     DAWHXZ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.340"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..150
                     /locus_tag="WIE35_002071"
     CDS             <1..150
                     /locus_tag="WIE35_002071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154938.1"
                     /translation="SIRQGKPEEEFSRKMAGCLTDLENCLEKYLEQFGPVSESIEACT
                     VKLQE"
     gene            172..465
                     /locus_tag="WIE35_002072"
     CDS             172..465
                     /locus_tag="WIE35_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154939.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKERIALFPRGLNGITKLPAAVLLPERPDCFDMKAVWQMFNRIPR"
BASE COUNT          158 a          150 c          160 g          130 t
ORIGIN      
        1 tccattcggc agggaaagcc tgaagaagaa ttttcccgta agatggccgg ctgcctgacc
       61 gacttggaaa actgtttgga aaaatacctc gaacagttcg ggccggtatc ggaaagtatc
      121 gaagcctgta ccgtcaaact gcaggagtaa ccgtcgttct tcaacaggct gatgaaaacc
      181 aacgacaagc tcaataggca gattgacgtg ttgcagaaac agtcggcagc catccacaac
      241 gaagcctata tcgaaatgaa cacgctgctt taccgccacc gcgaagtggt ttccgtccac
      301 aaccgcaagg cggattatgc ggaaaagggc aaagagcgga ttgcgctgtt cccgcgcggc
      361 ttgaacggca tcaccaagct gcctgccgcc gtcctgttgc ccgagcgtcc cgattgtttt
      421 gatatgaagg cagtttggca aatgtttaat agaataccga ggtaggcgta tcggctgccg
      481 gataaaaatg ccgtctgaaa cggaagcgtt cagacggcat tgttgcagtg gaacaaatca
      541 aaatgcaccc cgtcattcct gtctgtacgg tgatagcggc agtgttttcc tgcttcgt
//
LOCUS       DAWHXZ010000341          594 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.341, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000341 DAWHXZ010000000
VERSION     DAWHXZ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.341"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..554
                     /locus_tag="WIE35_002073"
     CDS             <1..554
                     /locus_tag="WIE35_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ4154940.1"
                     /translation="ERFFPQPRETAALRAKYGFADHETVFLFPSTGHTRKGLELLADF
                     FEHTDLPVKLAVVGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVE
                     SVLCGTRVVLSENMACTEVMNEEAGFFFSRQNPETLAQAVAQAVSLKKQGGHRLSDPM
                     RALNYNPSLSHHIDRLTDILASV"
BASE COUNT          133 a          204 c          133 g          124 t
ORIGIN      
        1 cggaacgctt ctttccacaa cctagagaaa ctgccgctct gcgcgccaaa tacggttttg
       61 ccgaccatga aaccgttttc ctatttccat cgaccggcca cacgcgcaaa ggcctggaac
      121 tgcttgccga ctttttcgaa cataccgacc tgcccgtcaa gcttgccgtt gtcggctccc
      181 cgctcccccg ccctatgaaa aacgtcgtcg gactgggctt ctgcaccgat atgcccgaac
      241 tctaccgcgc cgccgacttt accattatgg cctccctgta tgaacccttc gggctggtcg
      301 gtgtcgaatc cgtcctatgc ggcacacgcg tcgtcctctc cgaaaacatg gcatgtacag
      361 aggtcatgaa cgaagaagcc ggattctttt tctcacgcca aaacccggaa accctagcgc
      421 aagccgttgc ccaagccgtc agccttaaaa aacagggcgg acaccgcctg tccgacccga
      481 tgcgtgcact gaactacaac ccgtccctgt cgcaccacat cgaccgactg accgacatac
      541 tggcatccgt ataacaaaat gccgtctgaa cgagttttca gacggcattg ctgc
//
LOCUS       DAWHXZ010000342          588 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.342, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000342 DAWHXZ010000000
VERSION     DAWHXZ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.342"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..272)
                     /locus_tag="WIE35_002074"
     CDS             complement(<1..272)
                     /locus_tag="WIE35_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ4154941.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQLQLTEPL
                     PNIQPQVTKRKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDV"
BASE COUNT          166 a          152 c          115 g          155 t
ORIGIN      
        1 acgtcttgct caagctgcca aacagccttc agcccttcgc ccaaatcctc actcccctta
       61 aagccgataa acgagccgaa atcgctgatt ttcgtcctga tgcggctttt gcgcttagta
      121 acctgaggtt gaatatttgg gagcggttcg gtcaactgca gctggatgtt cctgccttcc
      181 acgccggctt tgatttcgcc gtacaggctg acatcggcaa cggccgcaag cggcagtgcg
      241 gacaatacga gggcggtaag tttttttcgc atatcggctt ccttttgtaa atttgataaa
      301 aacctaaaaa catcgggcaa acacccgata tgtcatcaat tatacccccc ccccgcaaaa
      361 aaccattttt cagaacaaat atctgataaa tgccgcaacc tttattttaa aaatgattat
      421 attttgatat aaaacaatag cttatttttc aaaaaacgtt gtgtttctac aacacaattc
      481 aagcgcagac ctcgtgcggg ccgatgcgct gctgcccgga tgcagtctcg gctttttaaa
      541 acgccataaa aaaacacacg cggcacttta tagtggatta acaaaaac
//
LOCUS       DAWHXZ010000343          582 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.343, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000343 DAWHXZ010000000
VERSION     DAWHXZ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.343"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            32..394
                     /locus_tag="WIE35_002075"
     CDS             32..394
                     /locus_tag="WIE35_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ4154942.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSDKETFAKFPKIP"
     gene            425..>582
                     /locus_tag="WIE35_002076"
     CDS             425..>582
                     /locus_tag="WIE35_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ4154943.1"
                     /translation="MSTFFRQTAQAMIAKHINRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRP"
BASE COUNT          175 a          166 c          128 g          113 t
ORIGIN      
        1 cgcacaaatt tagtcaatat caagaccaat tatgaaccaa ctcgaccaac ttggcacccg
       61 tatcaacctg atttgcaatg tcttcgacaa atggatcggg cagcaggatc tgaattacaa
      121 cctctttgcc gtactttata ccctggcaac cgaaggcagc cgcacgcaaa agcatatcgg
      181 cgaaaagtgg agcctgccca aacagaccgt ttcaggcgta tgcaaaaccc ttgccggaca
      241 agggttgatt gaatggcagg aaagcgaaca ggaccggcgc gaacggttgc tgtcgctgac
      301 cgaaaaaggc aaagcccatg ccgcaccttt aacagaaagc gcgcaggaat tcagcgacaa
      361 agagaccttt gcaaaattcc ccaaaatccc ctaaattccc accaagacat ttaggggatt
      421 ttccatgagc accttcttcc ggcaaaccgc ccaagccatg attgccaaac acatcaaccg
      481 cttcccgcta ttgaagttgg accaggtgat tgattggcag ccgatcgaac agtatctgaa
      541 tcgtcaaaga acccgttacc ttagagacca ccgcggccgt cc
//
LOCUS       DAWHXZ010000344          571 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.344, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000344 DAWHXZ010000000
VERSION     DAWHXZ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.344"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(146..502)
                     /locus_tag="WIE35_002077"
                     /pseudo
     CDS             complement(146..502)
                     /locus_tag="WIE35_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            501..>571
                     /locus_tag="WIE35_002078"
     CDS             501..>571
                     /locus_tag="WIE35_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ4154944.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAP"
BASE COUNT          210 a          118 c           97 g          146 t
ORIGIN      
        1 gcctctaaaa aataatcgct tgttttgaca ccgatacact catatagtgg attaacaaaa
       61 atcaggacaa ggcgacgaag ccgcagacag tacagatagt acggcaaggc gaggcaacgc
      121 tgtactggtt taaatttaat ccactataca aatacagaaa ctcaagaaaa taaccttgtg
      181 tattaaccat ctcaagcaat tcagaaaaat caagaaattt tctgaccgta aacaaacgtt
      241 tccctaaaaa aacgatgtct tcaaaaatat cgaccaaata atcatcttcg ccgttttcat
      301 ttttatgggt taaaacaaca cgggaaaaat ctgttttcag atgcttgccc gcttgattgt
      361 tcggattatt gtccggaacg acaaaaccgt cctcaaaatc aaagtagacg ttgcgtcctt
      421 ctacctttat ctctgtgcaa taacaatcat gcagagaaat accatccgaa aaatttaggg
      481 gctgtactag attagcagat atgttaccct cgaaatatga agataacgca ctgcaaatta
      541 aagaaaaaag tacagaaaga actgctccgt t
//
LOCUS       DAWHXZ010000345          567 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.345, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000345 DAWHXZ010000000
VERSION     DAWHXZ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.345"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..136
                     /locus_tag="WIE35_002079"
     CDS             <1..136
                     /locus_tag="WIE35_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154945.1"
                     /translation="KTDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE
                     "
     gene            complement(412..>567)
                     /locus_tag="WIE35_002080"
     CDS             complement(412..>567)
                     /locus_tag="WIE35_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Sms; stabilizes the strand-invasion intermediate
                     during the DNA repair; involved in recombination of donor
                     DNA and plays an important role in DNA damage repair after
                     exposure to mutagenic agents; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ4154946.1"
                     /translation="ERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEAID
                     ICRDSRE"
BASE COUNT          137 a          162 c          135 g          133 t
ORIGIN      
        1 caaaacggac ggcaaagtac gcgccgccta taccgatacg cgcgccctca tcgccggcag
       61 ccgcatcggt tttgacgaag tggcaaacac tttggcaaac gccgaaaaac tgatacaaaa
      121 aaccgtaggc gaataagccc gtcgccgaca tatggcggat ggaaatcaaa tgcacacgcc
      181 cctcattccc acgaagtcgg gaatccaatc ggttgggtta ccgtgatttt caatcattgc
      241 agaaacggac gactccagat tcccaccctt gcggggatga cgggcgtgtg tgtttttgcc
      301 acatcacagg caactttgcc gcaaatctta tctgtcgccg gtatgccttc ttgtttcaat
      361 ccgccacaaa ccaaatgccg tctgaaaccc gatttcagac ggcatttccg tttattccct
      421 gctgtcgcgg caaatatcaa tggcttcctg caaactcgaa acgccgtaga ttttcaggtt
      481 cggaaactct ttggcattgc gcggcatatt ggctttggga acgatggcgc gtttaaagcc
      541 gagcttttcc gcttccttga gccgctc
//
LOCUS       DAWHXZ010000346          564 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.346, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000346 DAWHXZ010000000
VERSION     DAWHXZ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.346"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..454)
                     /locus_tag="WIE35_002081"
     CDS             complement(<1..454)
                     /locus_tag="WIE35_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ4154947.1"
                     /translation="MPSFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRERY
                     PFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSKYLIGGNLRLWQKPFWEHTI
                     RDEADFACHFDYLHFNPVKHGYVGQISDWRFSTFHRYVKQGIYPHNWGG"
BASE COUNT          157 a          131 c          130 g          146 t
ORIGIN      
        1 cgccacccca attatgcgga taaataccct gtttgacata acggtgaaac gtagaaaacc
       61 gccaatcgga aatttgtcct acatagccat gtttgaccgg attgaaatgc agataatcaa
      121 aatggcaggc aaaatcggcc tcatcgcgga tagtatgttc ccaaaagggt ttttgccaaa
      181 gcctgagatt gccgccgatt aaatatttgc tgtgccgctt gatctgccgc cagcgttccg
      241 aataagcaga atcattgtcc ggcagccgcc atatggtatg cagatggtcg ggcatcaaca
      301 cccatgccaa aatttcaaac ggataccgtt cgcgcaccgc cattaccgcc tgccgtaaag
      361 ccaaacgcac cgcatcatcg gtcaaaatct tctgccgttt attggttaca accgtaaaaa
      421 agtaagtgcc gccattgcgg taaaacgacg gtatttcata gtattatgct cggaatgatt
      481 ttgtaggtcg gattcttgaa ttcgacattt tgggcattgc tgcaatggat tgcaatgatg
      541 ggaatgttaa aggttttgtc ggat
//
LOCUS       DAWHXZ010000347          546 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.347, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000347 DAWHXZ010000000
VERSION     DAWHXZ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.347"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>546
                     /locus_tag="WIE35_002082"
     CDS             <1..>546
                     /locus_tag="WIE35_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ4154948.1"
                     /translation="LTGAGAAIIALAVTVVTSGAGTGAVLGLNGAAAAATDAAFASLA
                     SQASVSFINNKGDVGKTLKELGRSSTVKNLVVAAATAGVADKIGASALNNVSDKQWIN
                     NLTVNLANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIVH
                     KIAHAIAGCAAAAANKGKCQDG"
BASE COUNT          143 a          152 c          158 g           93 t
ORIGIN      
        1 cctaaccgga gccggagccg caattatcgc actggccgtt accgtggtca cctcaggcgc
       61 aggaaccgga gccgtattgg gattaaacgg tgcggccgcc gccgcaaccg atgcagcatt
      121 cgcctctttg gccagccagg cttccgtatc gttcatcaac aacaaaggcg atgtcggcaa
      181 aaccctgaaa gagctgggca gaagcagcac ggtgaaaaat ctggtggttg ccgccgctac
      241 cgcaggcgta gccgacaaaa tcggcgcttc ggcactgaac aatgtcagcg ataagcagtg
      301 gatcaacaac ctgaccgtca acctagccaa tgcgggcagt gccgcactga ttaataccgc
      361 tgtcaacggc ggcagcctga aagacaatct ggaagcgaat atccttgcgg ctttggtgaa
      421 tactgcgcat ggagaagcag ccagtaaaat caaacagttg gatcagcact acatagtcca
      481 caagattgcc catgccatag cgggctgtgc ggcagcggcg gcgaataagg gcaagtgtca
      541 ggacgg
//
LOCUS       DAWHXZ010000348          513 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.348, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000348 DAWHXZ010000000
VERSION     DAWHXZ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.348"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(128..>513)
                     /locus_tag="WIE35_002083"
     CDS             complement(128..>513)
                     /locus_tag="WIE35_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ4154949.1"
                     /translation="EAILLVEGQKSAVTEYYLNHGIWPSDNSAAGVASSADIKGKYVQ
                     KVEVNNGVITATMASSNVNNEIKGKKLSLWAKRQAGSVKWFCGQPVTRAAPNASADAV
                     NKVTGNEIDTKHLPSTAPTRKSTPN"
BASE COUNT           99 a          112 c          149 g          153 t
ORIGIN      
        1 gtgggaatga tgaaatttta agttttaaga atttatcgga aaaaacagaa accgctccgc
       61 cgtcattccc gcgcaggcgg gaatctagcc tcgtcggtgc ggaaacttat cgggtaaaaa
      121 ggtttctcta gtttggtgtc gattttcttg tcggtgctgt tgacggcagg tgcttggtgt
      181 cgatttcgtt gccggtgact ttgttaacgg cgtcggcgct ggcgttgggg gcggcgcgcg
      241 taaccggctg tccgcagaac cattttaccg aaccggcttg acgcttggcc cacagggaga
      301 gttttttgcc tttgatttca ttgtttacgt tgcttgaagc cattgtggcg gtaatgacgc
      361 cgttgttgac ttcaactttc tgaacatatt tgcctttgat gtcggcggag gatgccacgc
      421 cggcggcact attgtcggat ggccatatgc cgtgattcag gtaatactct gtgacggctg
      481 atttttgacc ttcgaccaaa aggatagctt cgt
//
LOCUS       DAWHXZ010000349          510 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.349, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000349 DAWHXZ010000000
VERSION     DAWHXZ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.349"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            24..431
                     /locus_tag="WIE35_002084"
     CDS             24..431
                     /locus_tag="WIE35_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ4154950.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
BASE COUNT          166 a           89 c          110 g          145 t
ORIGIN      
        1 gttgttgttt agaaggaggg aaaatgactt ataccactgc caaagccgcc gaaaaaatag
       61 gcatctccgc ccacacccta cgtttttacg acaaagaagg tttgttgcct aatatcggac
      121 gtgatgaata cggtaaccgc tgttttaccg ataacgattt gcaatggttg ggcttattgc
      181 aatgcttgaa aaatacggga atgagcttaa aagacatcaa acgctttgcg gaatgtaccg
      241 tcattggcga cgataccatt gaagaacgcc tttccttgtt tgaaaatcaa atagaaaatg
      301 tgaagtgtca aattgccgaa ttaaaacgct atttagattt gcttgaatac aaattggcgt
      361 tttaccaaaa agcgaaagca ttaggctcgg taaaagctgt aaatttgccg caaattcctg
      421 aaacggctta gttttagtgc aataagcagg atttttgtcc tgttcgtaat agaaatcaaa
      481 aatttgttgt ttaaaaagcg gattgtaagc
//
LOCUS       DAWHXZ010000350          496 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.350, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000350 DAWHXZ010000000
VERSION     DAWHXZ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.350"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            20..403
                     /locus_tag="WIE35_002085"
     CDS             20..403
                     /locus_tag="WIE35_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ4154951.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
BASE COUNT          109 a          125 c          127 g          135 t
ORIGIN      
        1 acattttgaa aggataccga tggaaccttc ctcctacgcg gcagaaaaaa aaggcaaagg
       61 cggcatcagg cgcgtcatta acgcattcgg ctattcgata gacggcatcg ccgccgccta
      121 ccgttacgaa gcggcattcc gtcaggtttt gtggctgaac gtactgctgg tgtgcgcggc
      181 atttttttgg gtttccgaaa ccgccgtccg cctgccgttg atcatcgcgt cttttgtgtc
      241 ggtcattgtc gaactgttca acaccgccgt cgaagctgcc gtcgatcata cttcgaccga
      301 aaaacacgag ctggccaaac gcgccaaaga cgcaggttct gccgcacaat tgttcgcgat
      361 gctgatgttg gcggcggttt ggctgtctgc cctgttcggg taaaacgctt gcagcaggat
      421 ttgtaatcct ttaggattag tatgtggtat ttattattca cttaatctac atcaaatttc
      481 caggcagtat ttgtat
//
LOCUS       DAWHXZ010000351          492 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.351, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000351 DAWHXZ010000000
VERSION     DAWHXZ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.351"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..305
                     /locus_tag="WIE35_002086"
     CDS             <1..305
                     /locus_tag="WIE35_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ4154952.1"
                     /translation="KQAQQMQENMKKAQAKLAETEIEGEAGNGLVKITMTCAHEVRKI
                     DISPDLIQEAADDKEMLEDLILAALKSARDKAEETANKTMGAFTQGLPPGVGDFFR"
BASE COUNT          164 a          134 c          117 g           77 t
ORIGIN      
        1 tgaaacaggc gcagcaaatg caggaaaata tgaaaaaagc acaagccaaa cttgccgaaa
       61 ccgaaatcga aggcgaagca ggcaacggct tggtcaaaat cacaatgacc tgcgcgcacg
      121 aagtacgcaa aatcgacatc agccccgatt tgattcaaga agccgccgac gacaaagaaa
      181 tgctcgaaga cctcatcctc gccgccctca aatccgcccg tgacaaagcc gaagaaaccg
      241 caaacaaaac aatgggcgca ttcacgcaag gtctaccccc cggagtgggc gacttcttcc
      301 gctgataccc gaccgtcatt cccacgcagg cgggaatcta gaacgtagaa tctaagaaac
      361 cgttttactc gataaatttc cgtgccgacg ggtctagatt cccgccttcg cgggaataac
      421 ggcatcagtt tgcaggattc ggcgtgaacg gtaaaaacag tgagaatgat aagaacgcaa
      481 aaacggcaag aa
//
LOCUS       DAWHXZ010000352          491 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.352, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000352 DAWHXZ010000000
VERSION     DAWHXZ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.352"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..439
                     /locus_tag="WIE35_002087"
     CDS             <1..439
                     /locus_tag="WIE35_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ4154953.1"
                     /translation="ASAVRRELAVSGIDIADLVLENGSGLSRKERVTARMMAQMLETA
                     YFSPFAQDFIDTLPIAGTDGTLRNRFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAV
                     VVIINSGRAVSLLPDLDNFVANNIISGGDGWLDAKLMCKERRA"
BASE COUNT          122 a          126 c          139 g          104 t
ORIGIN      
        1 ggcatctgcc gtccggcgcg aacttgccgt gtcgggcatc gatattgcgg atttggtttt
       61 ggaaaacggt tcaggcctgt ccagaaaaga aagggtaacg gcgagaatga tggcgcaaat
      121 gttggaaacg gcttatttca gcccgtttgc acaagatttc atcgatacgc tacccatcgc
      181 cggcacagac gggactttac gcaaccgctt caaacaaagc ggcgggctgt tgcgcttaaa
      241 aaccggcacg ctcaacaatg tccgcgccct tgcaggttat tggctgggcg acaaaccgat
      301 ggcggtggtc gtcatcatca acagcggccg cgccgtttcc ctgctgcccg acttggacaa
      361 cttcgttgcc aacaacatca tctccggcgg cgatggctgg ctggatgcga aactgatgtg
      421 caaagaacgc cgagcctgaa acaggaaaat atagtggatt aaatttaagg ggctgtccta
      481 gataactagg a
//
LOCUS       DAWHXZ010000353          490 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.353, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000353 DAWHXZ010000000
VERSION     DAWHXZ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.353"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1..>490)
                     /locus_tag="WIE35_002088"
     CDS             complement(1..>490)
                     /locus_tag="WIE35_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595-like element ISNme3 family transposase"
                     /protein_id="HEZ4154954.1"
                     /translation="SPLSGVVELDESYFGARRIRGKRGRGASGKTIVFGILKRNGVVY
                     TEIVPDASKASLIKVIRGHISADSEINTDGWKAYDGLVDMGYEKHYRVHHGSNEFARG
                     KQHINGIESFWSYTKGRLAKFHGISKEMFYLHLKETEFRFNHRHEDLGKILMKMLRNN
                     PI"
BASE COUNT          137 a          119 c           93 g          141 t
ORIGIN      
        1 ttaaattggg ttatttcgaa gcatcttcat taatatttta cctaaatctt catgccggtg
       61 gttaaaccta aactccgttt ctttgagatg cagataaaac atctctttgg aaataccatg
      121 aaatttagcc aaacgcccct tagtataact ccaaaaagat tcaataccat tgatatgttg
      181 ctttcctcga gcaaactcat tggaaccatg atggacacgg taatgcttct catagcccat
      241 atcgacaaga ccgtcatatg ctttccagcc gtcagtattg atttcactgt cagcagatat
      301 gtgaccacgt atgaccttga ttagtgaggc tttcgaagcg tcgggaacga tttccgtata
      361 gaccacgcca ttgcgtttca gtatgccaaa tacgatggtt ttacctgacg ctccgcgacc
      421 gcgcttaccc ctgatacgtc gcgcaccaaa gtaagattca tctaattcga ccacaccgga
      481 cagtggtgag
//
LOCUS       DAWHXZ010000354          480 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.354, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000354 DAWHXZ010000000
VERSION     DAWHXZ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.354"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(297..372)
                     /locus_tag="WIE35_002089"
     tRNA            complement(297..372)
                     /locus_tag="WIE35_002089"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(336..338),aa:Glu,seq:ttc)
BASE COUNT          129 a          114 c          121 g          116 t
ORIGIN      
        1 gtctgaaaat tcagaagttc tagattcccg ttttcacggg aatgacgaag ggttgtggga
       61 atgacgaagt ttcagacggc atcaaatgct ttgtcaggct tcagacagca tcgtccaccc
      121 tctgccgtca ttcccgcgca ggcgggaatc cagccttgtc ggcacggaaa cttatcggat
      181 aaaacggttt cttcaaccct acgctctaga ttcccgtttt cacgggaatg acgaagagtg
      241 tgagaatgac ggttcataaa taaaaaaacc tgcttaaaac aagcaggttt ttgaattggc
      301 acgcccacgg ggattcgaac cccggttgcc gccgtgaaag ggcagtgtcc taggcctcta
      361 gacgatgggc gcgtgggaat gggcattata catacttcta gaaatttagc aatacgatgc
      421 cgtctgaaac ttcgtcattc ccgcgaaagc gggaatctag aatctccaag ttttcagaca
//
LOCUS       DAWHXZ010000355          474 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.355, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000355 DAWHXZ010000000
VERSION     DAWHXZ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.355"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(162..474)
                     /locus_tag="WIE35_002090"
                     /pseudo
     CDS             complement(162..474)
                     /locus_tag="WIE35_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT           83 a          101 c          134 g          156 t
ORIGIN      
        1 tttaggttgc tgtttttggt tttctgtttt tgtgggaatg atgaaatttt aagttttagg
       61 aatttatcga aaaaacagaa accgctccgc cgtcattccc gcgcaggcgg gaatccagcc
      121 tcgtcggtgc ggaaacttat cgggtaaaaa ggtttctcta gtttggtgtc gattttcttg
      181 tcggtgccgt tgtcggcggt aacgtcgtcg ttggtggctt tggcgtcgtc gcgcgtaacc
      241 ggctgtccgc agaaccattt taccgaaccg tcttgacgct tggcccacag ggagagtttt
      301 ttgcctttga tttcgttgtt tacgccgctt gaaagcattg tggcggtaac gacgccgttt
      361 ttgacttcaa cgctttgaac atatttgcct ttgatgttgg cggaggttgc cacgccggca
      421 gaactgttgt tggcgggcca ttcgccgtga ttcaggtaat actcgggtgt tttt
//
LOCUS       DAWHXZ010000356          425 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.356, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000356 DAWHXZ010000000
VERSION     DAWHXZ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.356"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(55..366)
                     /gene="rpsJ"
                     /locus_tag="WIE35_002091"
     CDS             complement(55..366)
                     /gene="rpsJ"
                     /locus_tag="WIE35_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ4154955.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHMNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
BASE COUNT          113 a           95 c           98 g          119 t
ORIGIN      
        1 ataacataaa aaatccccaa gcattgattg cttggggatt ttcaatagtc cgaattattg
       61 gactttgatt tctacatcaa caccggccgg caaatccagc ttcatcagcg catcggtagt
      121 tttatcggtc caatccacga tgtccatcag gcgcaagtgg gtgcggattt ccaattgttc
      181 acgggaagtt ttgttcatgt gcggagaacg cagaatgttg aaacgttcga ttttggtcgg
      241 caacggaatc ggacctttta caacggcacc ggtacgtttt gcagtttcaa cgatttcttg
      301 tgcagaacgg tcaatcaggg cgtaatcata agctttcagg cggatacgga ttttttggtt
      361 tgccatttat caatatcctt caattaagcg ataacagaag aaaccacgcc ggcacccacg
      421 gtacg
//
LOCUS       DAWHXZ010000357          418 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.357, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000357 DAWHXZ010000000
VERSION     DAWHXZ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.357"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          108 a          100 c          106 g          104 t
ORIGIN      
        1 acctggattc ccactttcgt gggaatgacg gtggaaaagt tgccgtgatt tcggataaat
       61 tttcgtaacg cataatttcc gttttacccg ataaatgccc gcaatctcaa atcccgtcat
      121 tccccaaaaa caaaaaatca aaaacagaaa tatcgtcatt cccgcgcagg cgggaatcta
      181 ggtctgtcgg tgcggaaacg tatcggataa aacggtttct tcagatttta cgttctggat
      241 tcccactttc gtgggaatga cgggatgtag gttcgtagga atgacgtggt gcaggtttcc
      301 gtgcggatgg attcgtcatt cccgcgcagg cgggaatcca gacccctgac gcggcgggaa
      361 tctatcggaa atgactgaaa ccccgagatt ccagattccc acttccgtgg gaatgacg
//
LOCUS       DAWHXZ010000358          407 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.359, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000358 DAWHXZ010000000
VERSION     DAWHXZ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.359"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(111..326)
                     /locus_tag="WIE35_002092"
     CDS             complement(111..326)
                     /locus_tag="WIE35_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154956.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(319..>407)
                     /locus_tag="WIE35_002093"
     CDS             complement(319..>407)
                     /locus_tag="WIE35_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ4154957.1"
                     /translation="VTMEKDLRRMLLFFKREAYVVILERDRV"
BASE COUNT          116 a          100 c          106 g           85 t
ORIGIN      
        1 ttcggcgtgt ttgccggcaa aaaagagcag gattgatgct gatgccgcct gaagcggggg
       61 cgttcagacg gcattgtgat gtttggggtg gggtccggtt caagtccgca tcagttgata
      121 aacccgtcgt ctttcatttt cttttcggaa aaatactggc cgaataccag cagggcagcg
      181 aggatgatga caaacagtcc cgccatagtc agagtgattt tgaaaaacaa ttccgcttcg
      241 agaaaagaaa accaaagttg cacaatcagc agcagcaccc agccggcaaa cagtccgaga
      301 caggcataaa cgccgagctt aaacacggtc ccgctccaaa atgacgacgt aggcctcgcg
      361 tttgaaaaac agcagcatac ggcgcaaatc tttttccatc gttacga
//
LOCUS       DAWHXZ010000359          406 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.360, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000359 DAWHXZ010000000
VERSION     DAWHXZ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.360"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            105..320
                     /gene="rpmE"
                     /locus_tag="WIE35_002094"
     CDS             105..320
                     /gene="rpmE"
                     /locus_tag="WIE35_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ4154958.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT          126 a          106 c           75 g           99 t
ORIGIN      
        1 ttatcgaata tcctcttgac gaaacaacag aaaatagcga gaatgccgag cttgtctgaa
       61 gcataggtac atcccctatg cccgcttttt tgaaaggaaa tcaaatgaaa caaggtattc
      121 acccgaacta ccacgaagtc aacgttacct gctcttgcgg caacaaattc gcaaccaaat
      181 ccgcgatgga aaaagaaaac ttcaatatcg aggtttgctc cctgtgccac ccgttctata
      241 ccggcaccca aaaaatcgtc gataccaccg gccgcgtgga caaattcaac aacaaattcg
      301 gcaacctgtt caaacgctga ttgccgcttt gcaagaatta agccctgcca tcatttcgat
      361 ggcagggctt tttaatattt ggcacactga cttcttatct atatag
//
LOCUS       DAWHXZ010000360          398 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.361, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000360 DAWHXZ010000000
VERSION     DAWHXZ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.361"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           78 a           87 c          117 g          116 t
ORIGIN      
        1 tagatttaat tcactatacc tgcgtaatgg tatcgggtac tcatcatcaa cctgccttgc
       61 attcggttgt gttgtccggc aactgtccgg ttgtgtcgat gggttgccct gccgctccct
      121 gtgctacgca acggttgtgt gccttgggtg ggcgcgggtg gcggccgggc ggtttgttgc
      181 tatgatacgg gagttccgat atacaagggt gggcttcagc ccaccaatca acgaccgaaa
      241 aagaaaaaac gttgggctga agcccactct acgcaattac gcaactgtta tcgtggggat
      301 gacgggattt taggtttttg tttttctgtt tttgcgggaa ggatgggttt taggttgcgg
      361 tcatttatcg gaaaagcaga aatcgctccg ccgtcatt
//
LOCUS       DAWHXZ010000361          397 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.362, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000361 DAWHXZ010000000
VERSION     DAWHXZ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.362"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          124 a           82 c           89 g          102 t
ORIGIN      
        1 cagtgtcatg agaaattttc ctttgtcaag tgttaaaatt ataatgatta tatactacat
       61 tatactacat cgcaatgaaa aatcaggaaa agcaaaaaac cctccgccgt cattcccgcg
      121 aaagcgggaa tctagaattt taatgccgca agaatttatc aaggatggtc ggggtttaaa
      181 ggtctggatt cccgcctgcg cgggaatgac ggtttagaaa tcacccgaaa caagaaaaag
      241 aaccgaaacc gaacaagccg gattcccgcc tgcgcgggaa tgacgggatt tcaggttact
      301 gtttttgatt ttctgttttt gtgggaataa cgggactttg gatgatgtgc atttatcgag
      361 aaaactaaaa accctccgcc gtcattcccg cgaaagc
//
LOCUS       DAWHXZ010000362          395 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.363, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000362 DAWHXZ010000000
VERSION     DAWHXZ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.363"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          105 a           86 c          105 g           99 t
ORIGIN      
        1 ctttcgtggg aatgacgaaa agtggcggga atgacgaaaa gtagcgggaa tgacgaaaag
       61 tggtgggaat gatggttcgg gcattcctta aatcccccgt gtatcgctgt aaatcttaga
      121 gatggcggaa tatagcggat taacaaaaac cagtacagcg ttgcctcgac ttagcttaaa
      181 gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact gtttgtattg
      241 tctgcagctt cgccgccttg tcctgatttt tgttaatcca ctataaagac cgtcgggcat
      301 ctgcggccgt cattcccgcg caggcgggaa tccagacctt aaggcagcgg caatattcaa
      361 agattatctg aaagtccgag attctggatt cccgc
//
LOCUS       DAWHXZ010000363          383 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.364, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000363 DAWHXZ010000000
VERSION     DAWHXZ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.364"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          108 a           85 c           95 g           95 t
ORIGIN      
        1 ggaaacgacc gaaaccgaac ggactggatt cccgcttttg cgggaatgac ggcgacaggg
       61 ttgctgttat agtggattaa caaaaaccgg tacggcgttg tctcgcctta gctcaaagag
      121 aacgattctc taaggtgctg aagcacgagt gaatcggttc cgtaccatct gtattgtctg
      181 cggcttcgtc gccttgtcct gatttttgtg aatccactat agaatttcaa tgcctcaaga
      241 atttatcgga aaaaaccaaa acccttccgc cgtcattctc acgaaagtgg gaatctagaa
      301 atgaaaagca gcaggaattt atcggaaacg accgaaactg aatggaccgg attcccgctt
      361 ttgcgggaat gacgaggttt taa
//
LOCUS       DAWHXZ010000364          381 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.365, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000364 DAWHXZ010000000
VERSION     DAWHXZ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.365"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..352
                     /locus_tag="WIE35_002095"
     CDS             <1..352
                     /locus_tag="WIE35_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ4154959.1"
                     /translation="EMKSSGVNKEIQGKKLSLWAKRQNGSVKWFCGQPVTRAANNTAK
                     DAVTAANDNSNNGINTKHLPSTCRDAASAICTRTPRADFKHFQKISRYRVLPESRHMA
                     EKLRHSRKSGNLVR"
BASE COUNT          124 a          110 c           79 g           68 t
ORIGIN      
        1 cgaaatgaaa tcaagcggcg taaacaaaga aatccaaggc aaaaaactct ccctgtgggc
       61 caagcgtcaa aacggttcgg taaaatggtt ctgcggacag ccggttacgc gcgccgccaa
      121 caacaccgcc aaagacgccg tcaccgccgc caacgacaac agcaacaacg gaatcaacac
      181 caaacacctg ccgtcaacct gccgcgatgc ggcatctgcc atttgcacaa gaacaccacg
      241 cgccgatttc aaacattttc aaaaaatcag ccgttaccga gtattgcccg aatcacggca
      301 catggccgaa aaacttcgtc attcccgcaa aagcgggaat ctagttcgtt gagtttcagt
      361 tatttagaat aaattttgaa a
//
LOCUS       DAWHXZ010000365          375 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.366, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000365 DAWHXZ010000000
VERSION     DAWHXZ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.366"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..313
                     /locus_tag="WIE35_002096"
     CDS             <1..313
                     /locus_tag="WIE35_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ4154960.1"
                     /translation="GARVAYGHVRYSIDSTKKTTDVVTVATNTPDGAPTIYNEPKTQN
                     AYHESHSIRRLGLGVVAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYR
                     F"
BASE COUNT           95 a          118 c           83 g           79 t
ORIGIN      
        1 cggtgcgcgc gtcgcctacg gacacgtcag atacagcatc gattcgacca aaaaaacaac
       61 agatgttgtt accgtcgcca ccaatactcc tgacggagca cctacgattt ataatgagcc
      121 aaagacgcaa aacgcctatc acgaaagcca cagcatccgc cgcttgggtc ttggtgtcgt
      181 cgccggtgtc ggtttcgaca tcacgcccaa gctgacttta gacaccggat accgttacca
      241 caactgggga cgcttggaaa acacccgctt caaaacccac gaagtctcat tgggcatgcg
      301 ctaccgcttc tgattccccg acaccgatgc cgtctgaacc ttcagacggc atttttgatt
      361 cacctgccgt ttaca
//
LOCUS       DAWHXZ010000366          368 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.367, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000366 DAWHXZ010000000
VERSION     DAWHXZ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.367"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..>368)
                     /locus_tag="WIE35_002097"
     CDS             complement(<1..>368)
                     /locus_tag="WIE35_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ4154961.1"
                     /translation="DLDFSCGAASVATLLNNFYGQTLTEEEVLKKLDKEQMRASFEDM
                     RRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSL
                     GHVSMSRAQFLDAWQTREGN"
BASE COUNT          101 a          113 c           73 g           81 t
ORIGIN      
        1 aaatttccct cacgggtttg ccaagcatcc aaaaactgcg ccctgctcat cgaaacatga
       61 cccagcgacg ggtcggcaag caaaaccgta ttgccgtcta taccgcgcaa taccgagaaa
      121 tgatcatcct tacggtattt cagatacacg atgacgggga ttttcaactg cgcgagctgc
      181 tcgaaagaca gggcatagcc tttcgcttca aaacccaaat ctggcataat gcgccgcata
      241 tcctcaaacg acgcgcgcat ctgctcctta tccagctttt tcaacacttc ttcttccgtc
      301 agcgtttgcc cgtaaaaatt gttcaaaagc gtcgccaccg aagccgcccc gcaggaaaaa
      361 tccaaatc
//
LOCUS       DAWHXZ010000367          365 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.368, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000367 DAWHXZ010000000
VERSION     DAWHXZ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.368"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          122 a           87 c           59 g           97 t
ORIGIN      
        1 tcttttctaa aatatgcaga tattcgaata tcgaatttgc cttatgatta cggttttctg
       61 tcttatctgc cttaaaacgc cggtattccg tttgaaccaa attatatttg ccgaaacact
      121 caaaaatctc ccttacctgc ccgacagaca tcaagccttc gttgttgtag ctgagaaaaa
      181 tatattgaaa gtccgcatcc ctaatcaaag cctcgaatac cggaaccaca tccgattttg
      241 agcaagtccg cgctaaaaca catcgtacag cgcaaaaccg gatgggaatg gcaaaatccg
      301 gaaacaaaca tttttcaaag catacaaaca aaaaaatacc aaccggaggt tggtattcct
      361 taact
//
LOCUS       DAWHXZ010000368          363 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.369, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000368 DAWHXZ010000000
VERSION     DAWHXZ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.369"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          113 a           97 c           67 g           86 t
ORIGIN      
        1 cagaacgtaa aatctcaaga aaccgtttta cccgataagt ttctgtgccg acagacctag
       61 attcccgcct gcgcgggaat gacgggattt gagattgcgg catttatcgg gagcaacaga
      121 atccgctctg ccgtcattcc cacgaaagtg ggaatccagg acgcaaaatc tcaagaaacc
      181 gttttgcccg ataagtttcc gcaccgacag acctagattc ccgcctgcgt gggaatgacg
      241 gcggaaagat tctatttttc ccgataatca cccacaatct caaattcctt cattctctca
      301 aaaacaaaat cagaacccta aatcccatca tccccatcta tgtgaatata aaaattttaa
      361 aaa
//
LOCUS       DAWHXZ010000369          360 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.370, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000369 DAWHXZ010000000
VERSION     DAWHXZ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.370"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            19..213
                     /gene="thiS"
                     /locus_tag="WIE35_002098"
     CDS             19..213
                     /gene="thiS"
                     /locus_tag="WIE35_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ4154962.1"
                     /translation="MNIILNGEPAELHGTTVADLIAQTAPQKPFAVAVNTGFVAKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT          101 a           99 c           88 g           72 t
ORIGIN      
        1 cagaaacatc gagaaaccat gaacatcatc ttaaacggcg aacccgccga acttcacggc
       61 acgaccgttg ccgacctcat cgcccaaacc gcgccgcaaa agccttttgc cgtagcggtg
      121 aataccggat ttgtcgccaa aggcgcgtat gcggaaaccg ttttgaacga aaacgacaaa
      181 atcgatatcg tgcggccggt ggtcggcggc taggcggttt tgccttttca gacgacccct
      241 gtccccaaaa caacgttatg gtggattaac tttaaatcag gacaaggcga cgaagccgca
      301 gacagtacaa atagtacgga accgattcac ttggtgcttc agcaccttag agaatcgttt
//
LOCUS       DAWHXZ010000370          357 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.371, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000370 DAWHXZ010000000
VERSION     DAWHXZ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.371"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          125 a           34 c           75 g          123 t
ORIGIN      
        1 tgatttttgt taatccacta tacgtcctag attcccactt tcgtgggaat gacgggatgt
       61 gggtttttgt gcggatttga accggtaagg gtggtgtggg attggtggtt tgcttaggat
      121 cttttggatt gtagtttgta tatacattta cttgttgata aaagataaaa taaaattaga
      181 aactaaaagt gagaaaaaat taataataat agggatgtat aaatgtaaag gctccgtttc
      241 atagctaagg ttatctgaat atatggaaaa aaagtaaaag tccataaaac taaaatatat
      301 agataatgct aatgataata gaattatcac tttcctagag taaggataat atttttt
//
LOCUS       DAWHXZ010000371          352 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.372, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000371 DAWHXZ010000000
VERSION     DAWHXZ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.372"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           96 a           61 c           76 g          119 t
ORIGIN      
        1 acgatgtgtt gatatttaat tttagaaagg tagctattta atagttacct tttcttattt
       61 aaaaatagct ttctcaaatt ccatgaacgc ctcaatacga tatgcagatg ctctatcgaa
      121 attaagtttc aacattttgt ttattaaaca ttttatttta gccatttttc aatatacccc
      181 caaatatacc cccaatttgc acaagtcaaa attttaggca ggggttttgt tgctcccatg
      241 atacgcgggc ggatgccggc ttttcgcggg ggaaatacaa ggggtctcgg ttcgggtgtc
      301 aaaatccctg tttcgtgtta gtcatgtggg ggggaagagg gggttagaat ga
//
LOCUS       DAWHXZ010000372          352 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.373, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000372 DAWHXZ010000000
VERSION     DAWHXZ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.373"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          120 a           85 c           72 g           75 t
ORIGIN      
        1 gaattagatt cccgcctgcg cgggaatgac aactcattag ttacctaaca cttaaaaaac
       61 agaaaccttt ccgcgtcatt cccacgaaag tgggaatccg ggaacttaac gttacagtga
      121 tttatcggaa acgactgaaa ccgaagagac cggattcccg cctgcgtggg aatgacaact
      181 cattagttac ctaaaactta aaaaacagaa acctttacgc cgtcattccc acgaaagtgg
      241 gaatccggga acttaacgtt acgacgattt atcggaaacg actgaaaccg aagagaccgg
      301 attcccgcct gcgtgggaac gacaactcat tagttactta aaacttaaaa aa
//
LOCUS       DAWHXZ010000373          347 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.374, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000373 DAWHXZ010000000
VERSION     DAWHXZ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.374"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           73 a           75 c           85 g          114 t
ORIGIN      
        1 gatatttata gtggattaac aaaaaccggt acggcgttgt ctcgccttag ctcaaagaga
       61 acgattctct aaggtgctga agcaccaagt gaatcggttc cgtactgttt gtactgtctg
      121 tggctcgccg ccttgtcctg atttttgtta atctgctata aagaccgtcg ggcatctgca
      181 gccgtcattc ccgcgcaggc gggaatctag gtctgttcgg ttccagttat ttccgataaa
      241 tgcctgttgc ttttcatttc tagattccca ctttcgtggg aatgacggtt cagttactac
      301 ggttactgtc aggtttcggt tatgttggaa tttcgggaaa cttatga
//
LOCUS       DAWHXZ010000374          345 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.375, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000374 DAWHXZ010000000
VERSION     DAWHXZ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.375"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..306
                     /locus_tag="WIE35_002099"
     CDS             <1..306
                     /locus_tag="WIE35_002099"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ4154963.1"
                     /translation="EQCKNISQTAATFNLSRNTLYLWIRLKKQTGSLKHQVTGLNAVK
                     SDRQKPAQYVGQHPDAYLHEIAKHFDCTAATICYALKQMGITRKKRPPLTKNKTRPK"
BASE COUNT          123 a           92 c           64 g           66 t
ORIGIN      
        1 gaacaatgca aaaacatcag ccaaaccgca gcaacgttta acttgtcaag aaacacgctt
       61 tacctgtgga ttcgccttaa aaaacaaaca ggcagcctaa aacatcaagt taccggtcta
      121 aatgccgtca aatcggatag gcaaaaaccg gctcaatatg ttgggcaaca cccggatgcc
      181 tatctgcatg aaatcgccaa acattttgat tgtacggcag ccaccatttg ctatgcactc
      241 aaacagatgg ggataacgcg caaaaaaaga ccaccactta caaagaacaa gacccggcca
      301 aagtaacgca ttatttgaca cagccggccg aattttccga ctacc
//
LOCUS       DAWHXZ010000375          332 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.376, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000375 DAWHXZ010000000
VERSION     DAWHXZ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.376"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           96 a           73 c           71 g           92 t
ORIGIN      
        1 tttttgaatt tcgggcaacg cttgaatctt cattccgcgc aggcggaaat tatcggtgcg
       61 gtacggcaac ttttttcgat atgaaaagac cgtcattcct gtaaaaacaa aaaatcaaaa
      121 acagaaaatt gaaattcgtc attcccgcgc aggcgggaat ctaggacgta aaatctatag
      181 tggattaaca aaaaccagta cagcgttgcc ttgacttagc tcgaagagaa cgattctcta
      241 aggtgctgaa gcaccgagtg agtcggttcc gcactatccg tactgtctgc ggcttcgtcg
      301 ctttctcctg atttttgtta atccactata aa
//
LOCUS       DAWHXZ010000376          330 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.377, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000376 DAWHXZ010000000
VERSION     DAWHXZ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.377"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           85 a           57 c           94 g           94 t
ORIGIN      
        1 cgggatttca ggttgctgtt tttgattttc tgtttttgtg ggaataacgg gactttggat
       61 gatgtgcatt tatcgggaaa acaaaaaacc ctccgccgtc attcccgcga aagcgggaat
      121 ctagaatttt aatgctgcaa gaatttatcg gggatgaccg aggtttgaag gtctggattc
      181 ccgcctgcgc gggaatgacg gtttagaaat cacctgaaac gagaaaaaga accgaaaccg
      241 gacaagtcgg attcccgcac gggcgggaag gacggaattt cagattgttg tttttgattt
      301 tctgttttgt gggaataacg ggattttgga
//
LOCUS       DAWHXZ010000377          312 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.378, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000377 DAWHXZ010000000
VERSION     DAWHXZ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.378"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          102 a           69 c           52 g           89 t
ORIGIN      
        1 cgcaaaagtc aatttgcatg gggttaattc aaaatccgcc cttcatctgc ccgcttccct
       61 aaaaaaaacc gtatatatat aactgtctgc attctatcgc tccggcgacg atacccatat
      121 ttccaagttt gtgtatcaaa attgtatatc gggcatagac tatttcggcg aggacgaaga
      181 tatagatttc cacgattgaa tacatggaag ccaagtacgt ctatcaacac tatattaaaa
      241 cacagccttt tttttgaggt ttcgggtaac ttttaaaccg tcattcctac gaaaacagaa
      301 aatcaaaaac ag
//
LOCUS       DAWHXZ010000378          310 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.379, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000378 DAWHXZ010000000
VERSION     DAWHXZ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.379"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          116 a           75 c           62 g           57 t
ORIGIN      
        1 aatccaaaaa tccaaaatca caggaagtta tcaaaaaaac agaaaccatc cgccgtcgtt
       61 cccgcgaaag cgggaatccg gaaccggaaa ccacaaaaat ttatcgggaa aaccgaaact
      121 caaaaacaac ggaaaccgaa cggacaggat tcccgcctgc gcgggaatga cggctcatac
      181 atcacccaaa aaattgaaac caaacagatg ggattccctc ttgcgtgagg ctgacagatg
      241 ccgtctgaaa gactttcaga cggcatagct ttttctcttt gaatttatag tggattaaca
      301 aaaatcagaa
//
LOCUS       DAWHXZ010000379          303 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.380, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000379 DAWHXZ010000000
VERSION     DAWHXZ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.380"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>303
                     /locus_tag="WIE35_002100"
     CDS             <1..>303
                     /locus_tag="WIE35_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Opacity protein opA54"
                     /protein_id="HEZ4154964.1"
                     /translation="LAYAAERITHDYPKPTGTDKDKISTVSDYFRNIRAHSIHPRVSV
                     GYDFGGWRIAADYASYRKWNNNKYSVNTKEVLRNNNGNRKELKTENQENGTFHAVSS"
BASE COUNT          108 a           74 c           66 g           55 t
ORIGIN      
        1 tttagcctac gccgccgaac gtattaccca cgattatccg aaaccaaccg gtacagacaa
       61 agacaaaata agcacagtaa gcgattattt cagaaacatc cgtgcgcatt ccatccaccc
      121 ccgggtgtcg gtcggctacg atttcggcgg ctggaggata gcggcagatt atgccagtta
      181 cagaaaatgg aacaacaata aatattccgt caacacaaaa gaggtgctaa gaaacaataa
      241 tggcaacagg aaagaactga agacggaaaa tcaggaaaac ggtacattcc acgcagtctc
      301 atc
//
LOCUS       DAWHXZ010000380          302 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.381, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000380 DAWHXZ010000000
VERSION     DAWHXZ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.381"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            82..>302
                     /locus_tag="WIE35_002101"
     CDS             82..>302
                     /locus_tag="WIE35_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEZ4154965.1"
                     /translation="MLRLTALTLCTVLALGACSPKDSDSAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTL"
BASE COUNT           65 a           98 c           65 g           74 t
ORIGIN      
        1 tatcatttat ccttttctaa agccgttccg gtttgtccga ccggcggctt tgccccaata
       61 tccccatttt ggagacaccc tatgttgcgt ttgaccgcat tgaccctctg caccgtcctc
      121 gccttgggcg cgtgttcgcc gaaagattcc gactctgccc cacaagccaa agaacaggca
      181 gtttccgccg cacaatccga aagcgcgtcc gttaccgtca aaaccgcgcg cggcgatgtt
      241 caaataccgc aaaaccccga acgtatcgcc gtttacgatt tgggtatgct cgacacttta
      301 aa
//
LOCUS       DAWHXZ010000381          301 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.382, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000381 DAWHXZ010000000
VERSION     DAWHXZ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.382"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>301
                     /locus_tag="WIE35_002102"
     CDS             <1..>301
                     /locus_tag="WIE35_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ4154966.1"
                     /translation="TGFNDAAGVASSSTIKGKYVKEVEVKNGVVTATMLSTGVNNEIK
                     GKKLSLWAKRQAGSVKWFCGQPVTRAANAKDDVAAATGTDKIDTKLKKPFYPISFR"
BASE COUNT           92 a           85 c           69 g           55 t
ORIGIN      
        1 acgggattta atgatgccgc cggcgtggca tcttcttcaa caatcaaagg caaatatgtt
       61 aaggaagttg aagtcaaaaa cggcgtcgtt accgccacaa tgctttcaac cggcgtaaac
      121 aacgaaatca aaggcaaaaa actctccctg tgggccaagc gtcaagccgg ttcggtaaaa
      181 tggttctgcg gacagccggt tacgcgcgcc gccaacgcca aagacgacgt tgccgccgcc
      241 accggcaccg acaaaatcga caccaaacta aagaaacctt tttacccgat aagtttccgc
      301 a
//
LOCUS       DAWHXZ010000382          299 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.383, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000382 DAWHXZ010000000
VERSION     DAWHXZ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.383"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            121..299
                     /locus_tag="WIE35_002103"
                     /pseudo
     CDS             121..299
                     /locus_tag="WIE35_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           80 a           81 c           66 g           72 t
ORIGIN      
        1 cgcgccttag ctcaaagaga acgattctct aaggtgctga agcaccaagt gaatcggttc
       61 cgtactattc gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
      121 actttatgcc gctaccgcaa ctggctggcg caagacaata ccctgtctga actgttggaa
      181 ctgattaacc gccaactgac cgaaaaaggt ttaaaaatag agaaagcatc cgccgccgtc
      241 attgacgcca ccattattca gaccgccggc agcaaacagc gtcaggccat agaagttga
//
LOCUS       DAWHXZ010000383          292 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.384, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000383 DAWHXZ010000000
VERSION     DAWHXZ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.384"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            31..201
                     /locus_tag="WIE35_002104"
     CDS             31..201
                     /locus_tag="WIE35_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ4154967.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT           84 a           79 c           76 g           53 t
ORIGIN      
        1 atccgctata caaaaaacaa ggaaacacaa atggcgcaat atatgtgcgg cccctgcggc
       61 tggatttacg atgaagaaca cggcgaccca gaacacggca tcccccccgg gacaaagttt
      121 gaagacattc ccgacgactg gaaatgcccc gaatgcggcg tgggcaaaga agatttctac
      181 ctgttggatt tcgtgatata gcggcagccg cacccttagg gaaaaccccg tcatccggcg
      241 ttccgcctgc ggcggcaggg aacgccgttt tatagtggat taacaaaaat ca
//
LOCUS       DAWHXZ010000384          287 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.385, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000384 DAWHXZ010000000
VERSION     DAWHXZ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.385"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>287
                     /locus_tag="WIE35_002105"
     CDS             <1..>287
                     /locus_tag="WIE35_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ4154968.1"
                     /translation="NTLSELLKLINRQLTEKGLKVEKASAAVVDATIIQTAGSKQRQA
                     IEVDNKGQVSGQTTPSKDKDARWTKKNSLYKLGYKQHTRTDAEGYIEKLYIT"
BASE COUNT          104 a           81 c           59 g           43 t
ORIGIN      
        1 aataccctgt ccgaattgct caaactgatt aaccgccaac tgaccgaaaa aggcctaaaa
       61 gtagagaaag catccgccgc cgtcgttgac gccaccatta ttcagaccgc cggcagcaaa
      121 cagcgtcagg ccatagaagt cgataacaaa ggacaagtca gcggccaaac cacaccgagt
      181 aaggacaaag atgcccgttg gacaaagaaa aacagcctct acaaactcgg ttacaaacaa
      241 catacccgta ccgatgcaga aggctatatc gagaaactgt acatcac
//
LOCUS       DAWHXZ010000385          275 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.386, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000385 DAWHXZ010000000
VERSION     DAWHXZ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.386"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..147
                     /locus_tag="WIE35_002106"
     CDS             <1..147
                     /locus_tag="WIE35_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ4154969.1"
                     /translation="SFGTLHRKFRYARSTYFGLIKVSAQSHLKAMCLNLLKAANRLSA
                     PAAA"
BASE COUNT           65 a           58 c           89 g           63 t
ORIGIN      
        1 agcttcggta cgctgcaccg taaattccgc tatgcgcggt caacctattt cggactgatt
       61 aaagtgagtg cgcaaagcca tctgaaggcg atgtgtttga acctgttgaa agccgccaac
      121 aggctaagtg cgcccgctgc cgcctaaaag gcagcacgga tgcctgatta tcgggtatcc
      181 ggggaggatt aagggggtat ttgggtagaa ttaggcggta tttggggcga aaacagccga
      241 aaacctgtgt ttgggtttcg gctgtcggga gggaa
//
LOCUS       DAWHXZ010000386          267 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.387, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000386 DAWHXZ010000000
VERSION     DAWHXZ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.387"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..222)
                     /locus_tag="WIE35_002107"
     CDS             complement(<1..222)
                     /locus_tag="WIE35_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ4154970.1"
                     /translation="MRPFFVGAAVLAILGALVFFINPGAIVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPVATLMAALLLAA"
BASE COUNT           83 a           79 c           66 g           39 t
ORIGIN      
        1 tgcggcgagc aataatgccg ccatcaaagt cgcgacaggt ttcaggttac ccgaaaaacc
       61 cgtccagtcc aacaaagccg cagtcaaaaa accgccgtat gccgccggca gcataagttc
      121 caagaaaatt tggcggtgca ggacgatggc accggggttg atgaaaaaca ccagcgcacc
      181 gagtatggca agcaccgccg cgccgacgaa aaacggccgc atagcaactg tatttttcac
      241 cccgtcgggc aaaaatacca aaactca
//
LOCUS       DAWHXZ010000387          264 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.388, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000387 DAWHXZ010000000
VERSION     DAWHXZ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.388"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            15..>264
                     /locus_tag="WIE35_002108"
     CDS             15..>264
                     /locus_tag="WIE35_002108"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154971.1"
                     /translation="MKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGFNGDI
                     GEKLHRCAVMEWAENETETFAKFLSLPTAETQTQVFSCF"
BASE COUNT           92 a           42 c           58 g           72 t
ORIGIN      
        1 taaaaaatat ccctttgaaa gattttgatg aagtttgtga aaagtgggag aaatccttta
       61 aagaaaatgg aaaagaaatt gaatataatc aaaaatattc aagaatttat ggtgaagttc
      121 gtggagctgg ctttaatggc gatatcggag aaaagctaca tcgttgtgcc gtgatggaat
      181 gggctgaaaa tgagaccgag acctttgcaa aattcctttc cctcccgaca gccgaaaccc
      241 aaacacaggt tttcagctgt tttc
//
LOCUS       DAWHXZ010000388          260 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.389, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000388 DAWHXZ010000000
VERSION     DAWHXZ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.389"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>260
                     /locus_tag="WIE35_002109"
     CDS             <1..>260
                     /locus_tag="WIE35_002109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4154972.1"
                     /translation="FGAKTAENSRKPVFGFRLSGGKGILQRSRALIAAKIDQAEISDS
                     EKSWLKKELGKIKDTALSTLTENAINAIPAATLITLLKKFVGL"
BASE COUNT           85 a           47 c           63 g           65 t
ORIGIN      
        1 tttggggcga aaacagctga aaacagccga aaacctgtgt ttgggtttcg gctgtcggga
       61 gggaaaggaa ttttgcaaag gtctcgggct ttaattgcgg caaaaataga ccaagcggag
      121 atttctgatt cagaaaaaag ctggttgaaa aaagaattag ggaaaatcaa ggacactgca
      181 ttaagtacgc ttacggaaaa tgcaattaat gctatccctg ccgccaccct cattactctt
      241 ttaaaaaaat ttgttggcct
//
LOCUS       DAWHXZ010000389          258 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.390, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000389 DAWHXZ010000000
VERSION     DAWHXZ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.390"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           72 a           58 c           66 g           62 t
ORIGIN      
        1 aaaacccttc cgtcatcatt cccgcaaaag cgggaatcta gaaatgaaaa gcaacaggaa
       61 tttatcggaa atgaccgaaa ctgaacggac tggattccct cttttgcggg aatgacggcg
      121 acagggttgc tgttatagtg gatgaacaaa aaccggtacg gcgttgtctc gccttagttc
      181 gaagagaacg attctctaag gtgctgaagc accaagtgaa tcggttccgt actatctgta
      241 ctgtctgcgg cttcgtcg
//
LOCUS       DAWHXZ010000390          258 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.391, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000390 DAWHXZ010000000
VERSION     DAWHXZ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.391"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            184..>258
                     /locus_tag="WIE35_002110"
     CDS             184..>258
                     /locus_tag="WIE35_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ4154973.1"
                     /translation="MLPSKYEDNTLQIKEKSTERTAPFF"
BASE COUNT           84 a           49 c           42 g           83 t
ORIGIN      
        1 actttgacaa cacacaagat atacgggaag gacagggcat cagcatatat ttcagcccga
       61 agccgtttca ttcgtttaaa aaactgcttt tttcacaatt tacctttaca aagcctatac
      121 ctatccttac aatttgtttt gctgtttttg gtatattttt ggggctgtac tagattagca
      181 gatatgttac cctcgaaata tgaagataac acactgcaaa ttaaagaaaa aagtacagaa
      241 agaactgctc cgtttttt
//
LOCUS       DAWHXZ010000391          257 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.392, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000391 DAWHXZ010000000
VERSION     DAWHXZ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.392"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           87 a           54 c           52 g           64 t
ORIGIN      
        1 tttttgttaa tccactataa tttattgaag atggctgaaa ttcaaagtta cagaaattta
       61 taaacaaaaa acagcaaccc gccgccgtca ttcccgcgaa agcgggaatc cagtcccttg
      121 accttaagat aatctttaat tcgtcattcc cgcaaaagcg agaatccgga agcgaaaagt
      181 ggtgggaatg tattagaaat gactgaaact caaaaaaccg gattcccgcc tgcgcgggaa
      241 tgacgattga tacgttt
//
LOCUS       DAWHXZ010000392          251 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02638
            SAMN17286480-rid13892603.denovo.393, whole genome shotgun sequence.
ACCESSION   DAWHXZ010000392 DAWHXZ010000000
VERSION     DAWHXZ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286480
            Sequence Read Archive: SRR13407196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242975.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:35:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,111
            CDSs (total)                      :: 2,058
            Genes (coding)                    :: 1,979
            CDSs (with protein)               :: 1,979
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 7 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286480-rid13892603.denovo.393"
                     /strain="NM02638"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(121..>251)
                     /locus_tag="WIE35_002111"
     CDS             complement(121..>251)
                     /locus_tag="WIE35_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEZ4154974.1"
                     /translation="LEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGAKG"
BASE COUNT           78 a           85 c           56 g           32 t
ORIGIN      
        1 ccgcgcaggc gggaatccac cacagggcaa caggaaaaca gaaaataaat aaggcagccg
       61 aaattcacca atggattccc gcctgcgcgg gaatgacggt aacaggtatt tcagacgacc
      121 tcaacccttc gcgcccgaac gcctgcccaa cgcccactgc atcacgttca gcgcaaacag
      181 aatcacaaac gaaaccagca gcataaacaa cgccaccgcc gacgcgccct gtacgtcgaa
      241 ctgttccagc t
//
